LOCUS       DAVGJJ010000001        72537 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000001 DAVGJJ010000000
VERSION     DAVGJJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.001"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            87..1112
                     /gene="trpS"
                     /locus_tag="VVE35_000001"
     CDS             87..1112
                     /gene="trpS"
                     /locus_tag="VVE35_000001"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU3060287.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1264..2742
                     /gene="guaB"
                     /locus_tag="VVE35_000002"
     CDS             1264..2742
                     /gene="guaB"
                     /locus_tag="VVE35_000002"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU3060288.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTNLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2793..3890)
                     /gene="recF"
                     /locus_tag="VVE35_000003"
     CDS             complement(2793..3890)
                     /gene="recF"
                     /locus_tag="VVE35_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU3060289.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3893..4261)
                     /gene="yaaA"
                     /locus_tag="VVE35_000004"
     CDS             complement(3893..4261)
                     /gene="yaaA"
                     /locus_tag="VVE35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU3060290.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4420..5670
                     /locus_tag="VVE35_000005"
     CDS             4420..5670
                     /locus_tag="VVE35_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU3060291.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5667..6950
                     /locus_tag="VVE35_000006"
     CDS             5667..6950
                     /locus_tag="VVE35_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU3060292.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSIMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            6992..7813
                     /locus_tag="VVE35_000007"
     CDS             6992..7813
                     /locus_tag="VVE35_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3060293.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7824..8369
                     /gene="pgsA"
                     /locus_tag="VVE35_000008"
     CDS             7824..8369
                     /gene="pgsA"
                     /locus_tag="VVE35_000008"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU3060294.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8366..9193
                     /locus_tag="VVE35_000009"
     CDS             8366..9193
                     /locus_tag="VVE35_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU3060295.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9178..10017
                     /locus_tag="VVE35_000010"
     CDS             9178..10017
                     /locus_tag="VVE35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU3060296.1"
                     /translation="MGIALKNVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10010..10804
                     /locus_tag="VVE35_000011"
     CDS             10010..10804
                     /locus_tag="VVE35_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3060297.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10863..11681
                     /gene="mreC"
                     /locus_tag="VVE35_000012"
     CDS             10863..11681
                     /gene="mreC"
                     /locus_tag="VVE35_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU3060298.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11681..12175
                     /gene="mreD"
                     /locus_tag="VVE35_000013"
     CDS             11681..12175
                     /gene="mreD"
                     /locus_tag="VVE35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU3060299.1"
                     /translation="MRQLKRVGVFLLLLFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12269..13447
                     /locus_tag="VVE35_000014"
     CDS             12269..13447
                     /locus_tag="VVE35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU3060300.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13671..14450
                     /gene="rpsB"
                     /locus_tag="VVE35_000015"
     CDS             13671..14450
                     /gene="rpsB"
                     /locus_tag="VVE35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU3060301.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            14529..15569
                     /locus_tag="VVE35_000016"
     CDS             14529..15569
                     /locus_tag="VVE35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU3060302.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15815..16102
                     /gene="tnpB"
                     /locus_tag="VVE35_000017"
     CDS             15815..16102
                     /gene="tnpB"
                     /locus_tag="VVE35_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU3060303.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(16293..16580)
                     /locus_tag="VVE35_000018"
                     /pseudo
     CDS             complement(16293..16580)
                     /locus_tag="VVE35_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16571..16969
                     /locus_tag="VVE35_000019"
     CDS             16571..16969
                     /locus_tag="VVE35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3060304.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEGWARM
                     GLPITRKEIVNWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17166..18086)
                     /gene="cysK"
                     /locus_tag="VVE35_000020"
     CDS             complement(17166..18086)
                     /gene="cysK"
                     /locus_tag="VVE35_000020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU3060305.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKSGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18371..19006)
                     /locus_tag="VVE35_000021"
     CDS             complement(18371..19006)
                     /locus_tag="VVE35_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU3060306.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19062..20360
                     /locus_tag="VVE35_000022"
     CDS             19062..20360
                     /locus_tag="VVE35_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3060307.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20357..21019
                     /locus_tag="VVE35_000023"
     CDS             20357..21019
                     /locus_tag="VVE35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU3060308.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21099..21647
                     /gene="raiA"
                     /locus_tag="VVE35_000024"
     CDS             21099..21647
                     /gene="raiA"
                     /locus_tag="VVE35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU3060309.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21783..23756
                     /locus_tag="VVE35_000025"
     CDS             21783..23756
                     /locus_tag="VVE35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU3060310.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23753..24205
                     /gene="rplI"
                     /locus_tag="VVE35_000026"
     CDS             23753..24205
                     /gene="rplI"
                     /locus_tag="VVE35_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU3060311.1"
                     /translation="MKVIFLANVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24249..25601
                     /gene="dnaB"
                     /locus_tag="VVE35_000027"
     CDS             24249..25601
                     /gene="dnaB"
                     /locus_tag="VVE35_000027"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU3060312.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25603..25869
                     /locus_tag="VVE35_000028"
     CDS             25603..25869
                     /locus_tag="VVE35_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060313.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25958..27304
                     /gene="cbpD"
                     /locus_tag="VVE35_000029"
     CDS             25958..27304
                     /gene="cbpD"
                     /locus_tag="VVE35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU3060314.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27387..27489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27560..27850
                     /locus_tag="VVE35_000030"
     CDS             27560..27850
                     /locus_tag="VVE35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU3060315.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27813..28571
                     /locus_tag="VVE35_000031"
     CDS             27813..28571
                     /locus_tag="VVE35_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060316.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            28609..29616
                     /locus_tag="VVE35_000032"
     CDS             28609..29616
                     /locus_tag="VVE35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3060317.1"
                     /translation="MKKTWKVFLTLVIALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29616..30344
                     /locus_tag="VVE35_000033"
     CDS             29616..30344
                     /locus_tag="VVE35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060318.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30463..30921
                     /locus_tag="VVE35_000034"
     CDS             30463..30921
                     /locus_tag="VVE35_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU3060319.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30923..33355
                     /locus_tag="VVE35_000035"
     CDS             30923..33355
                     /locus_tag="VVE35_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3060320.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTILSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            33676..34329
                     /gene="rr06"
                     /locus_tag="VVE35_000036"
     CDS             33676..34329
                     /gene="rr06"
                     /locus_tag="VVE35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU3060321.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34326..35666
                     /gene="hk06"
                     /locus_tag="VVE35_000037"
     CDS             34326..35666
                     /gene="hk06"
                     /locus_tag="VVE35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU3060322.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35749..36276
                     /locus_tag="VVE35_000038"
     CDS             35749..36276
                     /locus_tag="VVE35_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU3060323.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36352..38529
                     /gene="pspC"
                     /locus_tag="VVE35_000039"
     CDS             36352..38529
                     /gene="pspC"
                     /locus_tag="VVE35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU3060324.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFMGSVVHATEKEVTT
                     QVATSSNKANKSQTEHMKAAEQGKQHREIDLDRNKAKKAIDEYIEKMLSEIQLDKRKH
                     TQNFALNIKLSRIKTEYLYGLSVLKEKSEAELPSKAELPSKIKEKLTAAFEQFKKDTL
                     KPGEKVAEAKKKVEEAEKKAKDQKEEDRRNYPTITYKTLELEIAESDVEVKKAELELV
                     KEEANKPRNEQKVKQAKAKVESKQAEATRLKKIKTDREEAEEEAKRRAEAKLKEAVEK
                     NTATSEQGKPKRRGKRGALGEQATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVAEA
                     QKKVAEAEKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKEAELELVKEEAKESRN
                     EEKIKQVKAKVESKKAEATRLENIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPA
                     PQPEKPTEEPENPAPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRLTQQQPPK
                     IEKPAPAPQPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMA
                     TGWLQNNDSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWFQNNGSWYYLNANGD
                     MATGWLQYNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGSMATDWVKDGDTWYYLEAS
                     GAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(38759..39739)
                     /gene="dusB"
                     /locus_tag="VVE35_000040"
     CDS             complement(38759..39739)
                     /gene="dusB"
                     /locus_tag="VVE35_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU3060325.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(39726..40598)
                     /gene="hslO"
                     /locus_tag="VVE35_000041"
     CDS             complement(39726..40598)
                     /gene="hslO"
                     /locus_tag="VVE35_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU3060326.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            40763..42190
                     /locus_tag="VVE35_000042"
     CDS             40763..42190
                     /locus_tag="VVE35_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3060327.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            42349..43857
                     /gene="glpK"
                     /locus_tag="VVE35_000043"
     CDS             42349..43857
                     /gene="glpK"
                     /locus_tag="VVE35_000043"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU3060328.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEDAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            43899..45725
                     /gene="glpO"
                     /locus_tag="VVE35_000044"
     CDS             43899..45725
                     /gene="glpO"
                     /locus_tag="VVE35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU3060329.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKATLANNDLAELKN"
     gene            45793..46497
                     /locus_tag="VVE35_000045"
     CDS             45793..46497
                     /locus_tag="VVE35_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU3060330.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            46745..46891
                     /locus_tag="VVE35_000046"
     CDS             46745..46891
                     /locus_tag="VVE35_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060331.1"
                     /translation="MLVEFHKSQGTLETPEAQAEIAQKREEIEQRRAELEDKKQELLN
                     RLNK"
     gene            complement(46978..47238)
                     /locus_tag="VVE35_000047"
     CDS             complement(46978..47238)
                     /locus_tag="VVE35_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060332.1"
                     /translation="MTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(47293..47637)
                     /locus_tag="VVE35_000048"
     CDS             complement(47293..47637)
                     /locus_tag="VVE35_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060333.1"
                     /translation="MSKMFKCMCLLVMSVFCMSSISPSLVVFADDSLSANSKVVSGEA
                     QFENGSSVRFGDTQVNILSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEID
                     LNISFRSYDPNV"
     gene            48670..49947
                     /gene="ltrA"
                     /locus_tag="VVE35_000049"
     CDS             48670..49947
                     /gene="ltrA"
                     /locus_tag="VVE35_000049"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU3060334.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEITKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKFLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRHKTLVGTPQGGNLSPLLSNVMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSASRFIEKRLGLKVNMTKAKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     RIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            50404..51582
                     /locus_tag="VVE35_000050"
     CDS             50404..51582
                     /locus_tag="VVE35_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3060335.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            51896..52027
                     /locus_tag="VVE35_000051"
     CDS             51896..52027
                     /locus_tag="VVE35_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU3060336.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            52048..53598
                     /gene="dltA"
                     /locus_tag="VVE35_000052"
     CDS             52048..53598
                     /gene="dltA"
                     /locus_tag="VVE35_000052"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU3060337.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMSGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            53595..54839
                     /gene="dltB"
                     /locus_tag="VVE35_000053"
     CDS             53595..54839
                     /gene="dltB"
                     /locus_tag="VVE35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU3060338.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            54853..55092
                     /gene="dltC"
                     /locus_tag="VVE35_000054"
     CDS             54853..55092
                     /gene="dltC"
                     /locus_tag="VVE35_000054"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU3060339.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            55085..56353
                     /gene="dltD"
                     /locus_tag="VVE35_000055"
     CDS             55085..56353
                     /gene="dltD"
                     /locus_tag="VVE35_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU3060340.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLIQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            56508..56948
                     /gene="adcR"
                     /locus_tag="VVE35_000056"
     CDS             56508..56948
                     /gene="adcR"
                     /locus_tag="VVE35_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU3060341.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELACRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            56948..57652
                     /locus_tag="VVE35_000057"
     CDS             56948..57652
                     /locus_tag="VVE35_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU3060342.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            57645..58451
                     /locus_tag="VVE35_000058"
     CDS             57645..58451
                     /locus_tag="VVE35_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU3060343.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            58461..59966
                     /gene="adcA"
                     /locus_tag="VVE35_000059"
     CDS             58461..59966
                     /gene="adcA"
                     /locus_tag="VVE35_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU3060344.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(61135..61908)
                     /locus_tag="VVE35_000060"
     CDS             complement(61135..61908)
                     /locus_tag="VVE35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3060345.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            62084..63487
                     /locus_tag="VVE35_000061"
     CDS             62084..63487
                     /locus_tag="VVE35_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU3060346.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            63602..64240
                     /locus_tag="VVE35_000062"
     CDS             63602..64240
                     /locus_tag="VVE35_000062"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU3060347.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            64252..64692
                     /locus_tag="VVE35_000063"
     CDS             64252..64692
                     /locus_tag="VVE35_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU3060348.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVEKENV"
     gene            64685..65116
                     /locus_tag="VVE35_000064"
     CDS             64685..65116
                     /locus_tag="VVE35_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3060349.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            65107..65577
                     /locus_tag="VVE35_000065"
     CDS             65107..65577
                     /locus_tag="VVE35_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3060350.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            65602..66375
                     /locus_tag="VVE35_000066"
     CDS             65602..66375
                     /locus_tag="VVE35_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3060351.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            66372..67172
                     /locus_tag="VVE35_000067"
     CDS             66372..67172
                     /locus_tag="VVE35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3060352.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            67231..69525
                     /locus_tag="VVE35_000068"
     CDS             67231..69525
                     /locus_tag="VVE35_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU3060353.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTNFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            69535..72537
                     /locus_tag="VVE35_000069"
                     /pseudo
     CDS             69535..72537
                     /locus_tag="VVE35_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
BASE COUNT        22597 a        12159 c        15897 g        21884 t
ORIGIN      
        1 aagcaaggct gatttatttt gataaattac ggttatttca ttaaaaaaat gctataattg
       61 aaaggactat atcgaaggag aacaaaatga ctaaacccat tattttaaca ggagaccgtc
      121 caacaggaaa attgcatatt ggacattatg ttggaagtct caaaaatcga gtattattac
      181 aggaagagga taagtatgat atgtttgtgt tcttggctga ccaacaagcc ttgacagatc
      241 atgccaaaga tcctcaaacc attgtagagt ctatcggaaa tgtggctttg gattatcttg
      301 cagttggatt ggatccaaat aagtcaacta tttttattca aagccagatt ccagagttgg
      361 ctgagttgtc tatgtattat atgaatctag tttcgttagc acgtttggag cgaaatccaa
      421 cagtcaagac agagatttct cagaaaggat ttggagaaag cattccgaca ggattcttgg
      481 tctatccaat cgctcaagca gctgatatca cagctttcaa ggctaattat gttcctgttg
      541 ggacagatca gaaaccaatg attgagcaaa ctcgtgaaat tgttcgttct tttaacaatg
      601 catataactg tgatgtcttg gtagagccgg aaggtattta tccagaaaat gagagagcag
      661 ggcgtttgcc tggtttagat ggaaatgcta aaatgtctaa atcactaaat aatggtattt
      721 atttagctga tgatgcggat actttgcgta aaaaagtaat gagtatgtat acagatccag
      781 atcatatccg cgttgaggat ccaggtaaga ttgagggaaa tatggttttc cattatctag
      841 atgtttttgg tcgtccagaa gatgctcaag aaattgctga tatgaaagaa cgttatcaac
      901 gaggtggtct tggtgatgtg aagaccaagc gttatctact tgaaatatta gaacgtgaac
      961 tgggtcctat tcgtgagcgc cgtattgaat ttgctaagga tatgggagaa gtttataata
     1021 tgattcaaaa aggtagtgaa agagcgcgtg aagttgcggg tcaaacccta tctgaggtaa
     1081 aaggggcaat gggacttcat tactttaact aagtttattt tacaagaaac tatcatttct
     1141 atctcgaaga aaagatagtt ttttatttac ccctgtaaca tttgacaaat ttgtatagaa
     1201 tggtatgata gatacaatat aaaaagagtc aagctcaaaa agaaagaaaa gaggaaactt
     1261 cgaatgtcta attgggacac taaatttttg aaaaaaggtt ttacctttga tgatgtattg
     1321 cttattccag ctgaaagtca tgtgttgcct aacgatgcag atttaacaac taaattggca
     1381 gataatttga ctttaaatat cccaattatt accgctgcca tggacacagt tacagagagt
     1441 caaatggcca ttgctattgc tcgtgcaggt ggtctcggag ttatccataa aaacatgtca
     1501 attgctcaac aagcagacga ggttcgtaag gtaaaacgtt ctgaaaatgg agttattatt
     1561 gatccgttct tcttgacgcc tgaacataca attgctgaag cagatgagct tatgggtcgt
     1621 taccgcatca gtggtgttcc agttgttgaa acacttgaaa atcgtaaatt ggttggtatt
     1681 ttgacaaacc gagatcttcg ttttatttca gattataatc aaccaatttc aaaccatatg
     1741 actagtgaaa atcttgttac tgctcctgtg ggtacgaatc ttgcaacggc tgagagtatt
     1801 cttcaagagc atcgtattga aaaacttccg ttggtcgatg aagaaggcag tctttctggt
     1861 ttgatcacta tcaaagatat tgaaaaagtt attgagtttc caaatgcggc taaagatgag
     1921 tttggtcgtc ttctagttgc aggtgcagta ggtgttactt cagatacatt tgaacgtgca
     1981 gaggctcttt ttgaggcagg agcggatgcg attgttattg atactgcaca tggtcattct
     2041 gcaggtgtct tgcgtaaaat tgccgagatt cgtgctcatt tcccagatcg gactttgatt
     2101 gctggaaata ttgctactgc tgaaggtgca cgtgcccttt atgaagcggg tgtagacgtt
     2161 gttaaggttg gtattggacc aggttctatc tgtactactc gtgtgattgc tggtgttggt
     2221 gttccgcaag taacagctat ctacgatgct gcagctgttg cgcgcgaata tggtaaaacg
     2281 attattgctg acggtgggat caagtattct ggagatattg taaaagcact tgctgcaggt
     2341 ggaaatgctg ttatgcttgg atctatgttt gctggaactg atgaagctcc aggcgaaact
     2401 gaaatcttcc aaggacgtaa attcaagact taccgtggta tgggatcaat tgctgctatg
     2461 aagaaaggtt caagcgaccg ttatttccaa ggttctgtca atgaagcaaa caagcttgtt
     2521 ccagaaggaa ttgaaggtcg tgttgcttat aaaggagcgg cagctgatat tgttttccaa
     2581 atgattggtg gtattcgctc tggtatgggt tactgtggtg cagctaacct taaagaacta
     2641 cacgataatg ctcaatttat tgaaatgtct ggtgctggtt tgaaagaaag ccatcctcat
     2701 gatgtgcaaa ttactaatga ggcaccaaat tattctatgt aaaaaacaat gaaaagaact
     2761 ccagtgaaaa caggagttct tttacaatgt tgtcaatttc catttacagc agctttacca
     2821 tcctgaatag tgaagatact tagattttct ggcagatttt gaagatggtc taagcttgtt
     2881 gttgtgataa aggtttggat tgattgagaa atcgtttcta ataattttaa ctgtctagtg
     2941 ttgtcaagtt cactcatcac atcgtcaagc agtaatatcg gagattctgt ggtaatgctt
     3001 tccattaatt cgatttctgc taattttatc gagaggacga gactacgatg ttgaccttgg
     3061 cttccgaaac tagcatccat cccatttata taaaaagaaa tgtcatctcg atgaggaccg
     3121 acaccagtat tctttttaaa taaatctctg gatctacttt tttctaaagc aattttgaaa
     3181 gattcggata agttttgttt gtcagttata ttgacagaag attgatagga tattgacaac
     3241 tcttcgatct gattagagag ttcaaaatgt ttcttacgcc caaatgattc tagttttttt
     3301 atgaaatcta agcggtgatt cattacacga catccataat caactagctg atcatctaac
     3361 acagaaagga atgtttcatc tattttttga tctgatttta ggtaagtgtt tctttgcttt
     3421 aggatgtggt tataattggt taagtcagat aagtagattg gcttaatttg cccaagttcc
     3481 atatcaatga attttcgtcg aatcgaaggt gctcctttaa ttagttgtaa atcttcagga
     3541 gcaaataaga caacattcat gtgtcctaca taatctgaaa ggcgtgcctg ttttaagtga
     3601 ttaacttttg ttacacggcc tttttgtgtt agttcgattt ctaggggaat ggatccagtt
     3661 tttttctgaa cgagacctga aagatgaagt tgttcttcat caaaatgaat gagattttta
     3721 tctgttcgag ttcgatgact acgcgttaag gctaaaaaat agatagcctc taacatgttt
     3781 gtttttcctt gtgcattacg tcccaaaaag acattcaatt taggattaaa gtctattttc
     3841 gtttctttgt aattacgaaa agtcttgaga gatagatgtt gtagccacat gattatctac
     3901 cagggaatcg tggagcttgt tgacttttag gtgatgaagc aggtttcgat ttgtcttttt
     3961 taactccctt attcatcttt ttgacaagtt tagcgatccg ttctttttca actttatcag
     4021 cttggtattc atcttgttct tcagaagtag gttgtgtcaa caagatgtca atgttcatgt
     4081 cagggatgtc aactttatcg ccaatacgaa gtttttttcc acgacgactc tctaattccc
     4141 tattaaagta aacagaatgt tcagagagaa atgatttaat agctcctccg ctatgtgtaa
     4201 ttccaagttc tttgagtagt gcttggaggg taataaattc ttcaaataat ttgtattcca
     4261 taattcacct caatctttgg tattatacca tattttccta aaaatagtga gagagacaga
     4321 gagaaatgta agcgattttt atttcaaaag tgttacaggg aacaagaaaa tttcaggttt
     4381 tcgtgatata atagaagtct gtatataagg aggtaaatca tggagttagt gcatggaatt
     4441 tcaacacatt ttatccaatc aaaaaagttt aaaacaaaca aaattaccgt gcgttttacc
     4501 gctccattat cccttgatac gattgcaggt cacatgttga gtgcaagtat gctagagact
     4561 gctaatcaga tgtaccccac ttctcaagat ttgaggagac acttggccag tctatacggt
     4621 acagatatgt caaccaattg tttcagaaga gggcaaagcc acattataga attgacattt
     4681 acctatgttc gtgatgagtt tttaagtagg aaaaacgtgc taacctctca gattttggaa
     4741 cttgtaaaag aaactctttt ttcacccgta gtagttgata atgggtttga tccggcctta
     4801 tttgaaattg agaaaaaaca attgctagca agtttagcag ctgatatgga tgattctttt
     4861 tattttgcac ataaagaatt ggataaattg ttttttcatg atgaacgtct tcaattggaa
     4921 tatagtgatt tacgaaatcg tattttagct gaaactccac aaagttctta ttcttgtttc
     4981 caagaatttt tagccaatga tcgaatagat ttctttttcc taggtgattt taatgaggtt
     5041 gaaattcaaa atgtattaga atcatttggc tttaaaggtc gaaaaggaga tgtgaaggtt
     5101 cagtattgtc aaccttattc taatatcctt caggaaggta tggttcggaa aaatgtggga
     5161 caatccattt tggaattagg ttatcattac cgttctaaat atggtgatga gcaacattta
     5221 cccatgattg taatgaatgg tttacttggt ggatttgctc actctaagct ctttacaaat
     5281 gtccgtgaaa atgctggatt agcttatacc atttcaagtg agcttgattt atttagtgga
     5341 ttcttgagga tgtatgctgg tatcaatcga gaaaatcgta accaggctcg taaaatgatg
     5401 aataatcaac tgcttgattt aaaaaaaggt tattttacag agtttgagtt aaatcagacc
     5461 aaggaaatga ttcgttggtc gttgttactt tctcaagata atcaatcttc attgattgaa
     5521 cgtgcttatc aaaatgcctt atttggaaaa tcttcagcag actttaaaag ttggattgca
     5581 aagcttgaac aaattgacaa agatgctatt tgtagagtag ctaataatgt gaaactacaa
     5641 gcgatttact ttatggaagg aatagaatga caaaggttgt ttttgaagaa aaatactatc
     5701 cagctgtaaa agaaatggtt tatcgaactc gtttggccaa cggattgaca gttgctcttt
     5761 tgcctaaaaa ggaatttaaa gaggtttacg ggagtgtcac tgtacagttt ggttcggtag
     5821 atacgtttgt cacagaagtt gacggagatg taaaacaata tcctggagga attgctcatt
     5881 ttcttgaaca taaattattt gagagagaag attctagtga tttgatgtcg gcttttacga
     5941 gtctaggtgc agatagtaat gcctttacaa gctttacaaa aacaaactat cttttttcag
     6001 caacggatta ttttttagaa aatttagatt tacttgatga attggtaaca tcagcacact
     6061 ttactgaagc ttccattctg acagagcagg atattattca gcaagaacga gaaatgtacc
     6121 aagatgatcc agattcgtgt ttattctttt caactttagc gaatttgtat cctggtacac
     6181 ctttagcaac tgatatagtt ggaagtgagg agtccatttc ccaaatcaat ctaactaatt
     6241 tgcaagaaaa ttttacaaag ttttacaaac ctgtaaacat gtctctgttt ttagttggta
     6301 attttgatgt ggagcgagta caggactatt ttgaaagcaa agaactgaaa gattcagatt
     6361 ttcaggaagt agcaagagaa aagttgtttt tacagcctgt aaagccaaca gatagtatga
     6421 gaatggaagt atcttctccc aaactagcga ttggagttag aggtaagcga gaagtttctg
     6481 aagcggattg ctatcgacat catattttat taaaattatt gtttgcaatg atgtttggtt
     6541 ggacttcgga tcgttttcaa aaatgttatg aatcaggtaa aattgatgcg tccttatctc
     6601 tggaagttga aataacaagt cgctttcatt ttgtcatgtt gacaatggat acgaaagagc
     6661 cagttgcttt gtctcatcaa tttaggaagg ctattcgtaa ttttacaaag gatttagata
     6721 ttacagagga acatttagat attatcaaaa gagagatgtt tggcgaattt ttcagtatca
     6781 tgaactctct tgaatttatt gcaacgcaat atgatgcttt tgaaaacggt gagacaattt
     6841 ttgatttgcc gaaaatttta caggaaatta ctttagagga tgtccttgat gctggacatc
     6901 atttaataga tgatggtgac atagttgatt ttacaatatt cccatcgtag taacctatta
     6961 taatagacac tagaaagaag ggatgacaag tatgagaaaa aaaacaattg gagaggtttt
     7021 acgattagct agaatcaatc agggattgag tttagatgaa ttgcagaaaa agacagaaat
     7081 ccagttagat atgttggaag caatggaagc agacgatttc gatcaacttc caagtccttt
     7141 ttacacgcgt tctttcttga aaaaatatgc atgggctgtt gagttagatg accaaattgt
     7201 tttggatgct tatgattctg ggagtatgat tacttatgag gaagtagatg ttgatgaaga
     7261 tgagttgaca ggtcgtagac gttcaagtaa gaaaaagaag aaaaaaacat catttttacc
     7321 tttattttat tttatccttt ttgctttatc gattttaatt tttgtgactt attatgtttg
     7381 gaactatatt caaactcaac cagaggagcc ttctctttct aattacagtg tggttcaatc
     7441 aacaagttca actagctctg ttccccactc ctcaagtagt agttcttcta gtatagaatc
     7501 agctataagt gtatcaggcg aaggaaatca tgtagaaatc gcttataaga caagtaagga
     7561 aacagttaaa ttgcaattgg cagtttcaga tgttacaagt tgggtcagtg tttcagaaag
     7621 cgaacttgag ggcggtgtaa ccttatcgcc aaagaagaaa agtgcagaag caacagttgc
     7681 aactaaaagt cctgtaacaa ttacgttagg tgttgtaaaa ggtgttgatt tgacagtaga
     7741 taatcagact gttgatttat cgaaattaac agctcagact ggacaaatca ctgtaacctt
     7801 tactaaaaat taaggaaaaa cgaatgaaaa aagaacaaat tcccaatctc ttaacaatag
     7861 gtcgaattct ctttatacct atttttatct ttattttaac gataggaaat tcgatagaga
     7921 gtcatatagt tgcagctatt atctttgctg ttgccagtat taccgactat ttagatggat
     7981 atttagctcg taaatggaat gtggtcagta attttggtaa atttgcagat cctatggcgg
     8041 ataagttact agttatgtcg gcttttatta tgttgattga gttaggtatg gctccggctt
     8101 ggattgttgc agtgattatc tgtcgtgagt tagctgtgac aggtttaagg cttttattgg
     8161 ttgaaactgg tggaacaatt ttagcagcag caatgcctgg aaaaattaaa acttttagtc
     8221 agatgtttgc tattattttc ttgctattac attggacttt gcttggtcaa gttctacttt
     8281 atgtagcctt atttttcact atctactctg gctatgacta tttcaagggt agtgcctatg
     8341 tatttaaagg gacatttggt tcgaaatgaa atcaataatt gatgtaaaaa atctttcttt
     8401 tcgctataaa gaaaatcaga actactacga tgtgaaggat attacgtttc acgtgaaacg
     8461 tggagaatgg ctttcgattg tagggcataa tggtagtggt aaatcaacga cggttcgatt
     8521 aattgatggc ttactggaag cagaatccgg agagattgta attgatggcc aacgtttgac
     8581 tgaggaaaat gtttggaata tacgtcgtca aatcggtatg gtttttcaaa atccagacaa
     8641 tcaatttgtt ggagcgactg ttgaagatga tgttgctttt ggtttggaaa atcagggact
     8701 ttctcgtcaa gaaatgaaaa agagagtgga agaagctctg gctttagttg gcatgttgga
     8761 ctttaaaaag agagagccag cgcgtctatc aggtggccaa aagcaacgtg tggccattgc
     8821 aggtgttgta gccctaagac cagctatttt aatcttagat gaagcaacga gtatgttgga
     8881 tcctgagggg cgtagagaac ttattgggac agtaaaagga attcgaaaag actatgatat
     8941 gacagtcatt tctattaccc atgatttgga agaagtcgcc atgagtgatc gcgtattggt
     9001 catgaaaaaa ggggaaattg aatcaactag tagtccaagg gagcttttct ctcgaaatga
     9061 tttagatcaa attggattag acgatccttt tgctaatcaa ttaaaaaaat ctttgagcca
     9121 gaatggctat gatttacctg aaaattattt gacagaaagt gagctagagg ataagctatg
     9181 ggaattgctc taaaaaatgt gaattttaca tatcaagaag gtactccctt agcttcagca
     9241 gctttgtcgg atgtttcttt gacgattgaa gatggctctt atacagcttt aattgggcac
     9301 acaggtagtg gtaaatcaac tattttacaa ctcttaaatg gtttattggt gccaagtcaa
     9361 gggagtgtga gggtttttga taccttaatc acctcgactt ctaaaaataa agatattcgt
     9421 caaattagaa aacaggttgg cttggtattt cagtttgctg aaaatcagat ttttgaagaa
     9481 acggttttga aggacgttgc ttttggaccg caaaattttg gagtttctga agaagatgct
     9541 gtgaagactg cgcgtgagaa actggctctg gttggaattg atgaatcact ttttgatcgt
     9601 agtccgtttg agctgtcagg gggacaaatg agacgtgttg ccattgcagg catacttgcc
     9661 atggagccag ctatattagt cttagatgag ccaacagctg gtctagatcc tctagggaga
     9721 aaagagttga tgaccctgtt caaaaaactc caccagtcag ggatgaccat cgtcttggta
     9781 acgcatttga tggatgatgt tgctgaatat gcgaatcaag tctatgtaat ggaaaaggga
     9841 cgtttagtaa aggggggcaa accaagtgat gtctttcaag acgttgtttt tatggaagaa
     9901 gttcagttgg gagtacctaa aattacggcc ttttgtaaac gattggctga tagaggcgtg
     9961 tcatttaaac gattaccgat taagatagag gagttcaagg agtcgctaaa tggatagtat
    10021 gattttgggg cgttatatcc caggggattc gattgttcac cgattggatc cacgtagcaa
    10081 attgcttgct atgatgctac tgattttgat tgtgttttgg gctaataacc ccttgacgaa
    10141 tctgattctt tttatagcga cagggatatt tattgccttg tcaggagtat ctctttcatt
    10201 ttttattcag ggcttgaagt ctatgttttt cttgattgcc ttcacaacta tttttcaact
    10261 atttttcatt tctaatggga atgttttatt tgagttttcg tttgtgagaa tcacggatta
    10321 tgctttgcaa caagctggga ttattttttg tcgctttgta ttgattattt tcttttcaac
    10381 tttgttaacc ttaacaacca tgcctttaag tttggcatca gctgtcgaag ctttattagc
    10441 acctttaaag cgtgtgaaag ttccagttca tgaaattgga ttgatgctgt ctatgagttt
    10501 gcgttttgtc ccaaccttga tggatgatac gactcggatt atgaatgcac agaaagctcg
    10561 tggagtggat tttggagaag gaagcatcgt tcaaaaagta aaggcgatga ttcccatttt
    10621 gattcctctt tttgcgacaa gtttaaaacg tgcagattcc ttggctatcg ctatggaagc
    10681 gcgtggctat cagggtggaa aaggcagaag tcaataccga caattgaaat ggactctgaa
    10741 ggatacgctg accattcttg ttattctcgt acttggttgt tgtttatttt tcttaaaatc
    10801 ttagtagctt tcaggaattg ataaaaagtt actgtaacag tttttgatat aaaggtaggg
    10861 atatgaaccg ttttaaaaaa tcaaaatatg tcattattgt ttttgtcact gttctgcttg
    10921 tgtcagctct cttagcgacg acttattcaa gtacaattgt gacaaaatta ggagatggaa
    10981 tctcattggt tgatagagtt gtacaaaaac cttttcagtg gtttgattct gtcaaatcag
    11041 atttggctca tttgacacga acatataatg aaaatgaaag tttgaagaaa cagctttacc
    11101 aattagaagt taaatcaaat gaggtggaaa gtttaaagac agaaaatgaa caactgcgcc
    11161 aattgcttga tatgaagtct aaattgcaag ccacaaagac tttagcagca gatgttatta
    11221 tgcgttctcc ggtatcttgg aagcaggagt tgaccttaga tgcaggtaga tcaaaaggtg
    11281 cttctgagaa catgttagct attgcaaatg gtggcttgat tgggagtgtt tcaaaagtag
    11341 aggagaactc tactatagtc aaccttctga caaatacgga aaatgctgat aagatttctg
    11401 ttaaaattca acatggctct actacaattt atggaattat tattggctat gacaaggaaa
    11461 atgacgttct taaaattagc caattaaata gtaatagcga tattagtgcg ggagataagg
    11521 tgactactgg tggattagga aactttaacg ttgctgatat tcctgttggt gaagtggttg
    11581 ccacaacgca tagtacagac tatttgacac gagaagtaac tgttaaattg agtgcagata
    11641 ctcataatgt agatgtgata gaattagtgg ggaattcata atgagacagt tgaagcgagt
    11701 tggagtattt ttattgcttc ttttctttgt tctaattgac gcccatatta gccagcttct
    11761 gggctcattt ttcccccatg tacatttggc tagtcatttt ctttttctat ttctcttatt
    11821 tgagacgata gaagtatcag agtatctcta cctagtctat tgttttgtta taggcttggt
    11881 ttatgatgtt tactttttcc atctaatagg gattacaact ctcttattta tcttattggg
    11941 agccttcctt cataaattga atagtgttat tttgttgaat cgttggacaa gaatgctagc
    12001 tatgattgtg ctgacattcc tgtttgaaat gggtagttat cttttggctt ttatggtagg
    12061 gttgacagta gatagcatgt cgatttttat agtctatagc ttggtaccga cgatgatttt
    12121 aaatttttta tggattactg tttttcaatt tatttttgaa aaatattatc tataagaacg
    12181 acatataaat gtaacaaagg cgtaatattt attaggcctt tttttggtat actagtattg
    12241 tctttaaaag aaggagtatc tacgtaatat gaagaaaaaa atcttagcgt cacttttatt
    12301 aagtacagta atggtttctc aagtagctgt tttaacaact gcgcatgcag aaacgactga
    12361 tgacaaaatt gctgctcaag ataataaaat tagtaactta acagcacaac aacaagaagc
    12421 ccaaaaacaa gttgaccaaa ttcaggagca agtatcagct attcaagctg agcagtctaa
    12481 cttgcaagct gaaaatgata gattacaagc agaatctaag aaactcgagg gtgagattac
    12541 agaactttct aaaaacattg tttctcgtaa ccaatcgttg gaaaaacaag ctcgtagtgc
    12601 tcaaacaaat ggagccgtaa ctagctatat caataccatt gtaaactcaa aatcaattac
    12661 agaagctatt tcacgtgttg ctgcaatgag tgaaatcgta tctgcaaaca acaaaatgtt
    12721 agaacaacaa aaggcagata aaaaagctat ttctgaaaaa caagtagcaa ataatgatgc
    12781 tatcaatact gtaattgcta atcaacaaaa attggctgat gatgctcaag cattgactac
    12841 gaaacaggca gaactaaaag ctgctgaatt aagtcttgct gctgagaaag cgacagctga
    12901 aggggaaaaa gcaagtctat tagagcaaaa agcagcagct gaggcagagg ctcgtgcagc
    12961 tgcggtagca gaagcagctt ataaagaaaa acgagctagc caacaacaat cagtacttgc
    13021 ttcagcaaac actaacttaa cagctcaagt gcaagcagta tctgaatctg cagcagcacc
    13081 tgtccgtgca aaagttcgtc caacatacag tacaaacgct tcaagttatc caattggaga
    13141 atgtacatgg ggagtaaaaa cattggcacc ttgggctgga gactactggg gtaatggagc
    13201 acagtgggct acaagtgcag cagcagcagg tttccgtaca ggttcaacac ctcaagttgg
    13261 agcaattgca tgttggaatg atggtggata tggtcacgta gcggttgtta cagctgttga
    13321 atcaacaaca cgtatccaag tatcagaatc aaattatgca ggtaatcgta caattggaaa
    13381 tcaccgtgga tggttcaatc caacaacaac ttctgaaggt tttgttacat atatttatgc
    13441 agattaattt acagagggac tcgaatagag ccctcttttc aggttttacc gtgacaatcc
    13501 ctattaaaaa ttatatcaaa atagcttgaa aatattggaa aagtatggta gaatgaaaat
    13561 tgtcgtgtga acgataatac tcattcttga tgaattgtga agcagttgcc cttgggtcgt
    13621 tttgcgagtt gaagtcaaga agaggaaaaa aacaaaaagg agaaatactc atggcagtaa
    13681 tttcaatgaa acaacttctt gaggctggtg tacactttgg tcaccaaact cgtcgctgga
    13741 atcctaagat ggctaagtac atctttactg aacgtaacgg aatccacgtt atcgacttgc
    13801 aacaaactgt aaaatacgct gaccaagcat acgacttcat gcgtgatgca gcagctaacg
    13861 atgcagttgt attgttcgtt ggtactaaga aacaagcagc tgatgcagtt gctgaagaag
    13921 cagtacgttc aggtcaatac ttcatcaacc accgttggtt gggtggaact cttacaaact
    13981 ggggaacaat ccaaaaacgt atcgctcgtt tgaaagaaat taaacgtatg gaagaagatg
    14041 gaactttcga agttcttcct aagaaagaag ttgcacttct taacaaacaa cgtgcgcgtc
    14101 ttgaaaaatt cttgggcggt atcgaagata tgcctcgtat cccagatgtg atgtacgtag
    14161 ttgacccaca taaagagcaa atcgctgtta aagaagctaa aaaattggga atcccagttg
    14221 tagcgatggt tgacaccaat actgatccag atgatatcga tgtaatcatc ccagctaacg
    14281 atgacgctat ccgtgctgtt aaattgatca cagctaaatt ggctgacgct attatcgaag
    14341 gacgtcaagg tgaggatgca gtagcagttg aagcagaatt tgcagcttta gaaactcaag
    14401 cagattcaat tgaagaaatc gttgaagttg tagaaggcga caacgcttaa tttatacaaa
    14461 tagtaattac ctaggagggc ggggcttagc ccggctctcc tattttcaaa aaatatagga
    14521 gaattaaaat ggcagaaatt acagctaaac ttgtaaaaga gttgcgtgaa aaatctggtg
    14581 ccggtgttat ggacgctaaa aaagcgcttg tagaaacaga cggtgacatc gaaaaagcga
    14641 ttgaattgct tcgtgaaaaa ggtatggcta aggcagctaa gaaagctgac cgtgttgctg
    14701 cagaaggttt gactggtgtt tatgttaacg gtaatgttgc agcagttatt gaagtaaacg
    14761 ctgaaactga cttcgttgca aaaaacgctc aattcgttga attggtaaat actacagcta
    14821 aagtcattgc tgaaggaaaa cctgctaaca acgaagaagc tcttgctttg ataatgcctt
    14881 caggtgaaac tcttgaagct gcatacgtat ctgcaacagc aactatcgga gagaaaatct
    14941 cattccgtcg ctttgcattg attgaaaaaa cagacgcaca acactttgga gcataccaac
    15001 ataacggtgg acgtatcggt gttatttcag ttgttgaagg tggagacgaa gcacttgcta
    15061 aacaattgtc aatgcacatc gcagcgatga aaccaacagt tctttcttac aaagaattgg
    15121 atgagcaatt cgttaaagat gagttggcac aattgaatca cgttatcgac caagacaacg
    15181 aaagccgtgc aatggttaat aaaccagctc ttccacactt gaagtatgga tcaaaagctc
    15241 aattaactga tgatgttatt gctcaagctg aagctgacat caaagctgaa ttggctgcag
    15301 aaggcaaacc agaaaaaatc tgggacaaaa ttattccagg taaaatggat cgcttcatgc
    15361 ttgataacac taaagttgac caagcttaca cacttcttgc acaagtttac atcatggatg
    15421 acagcaagac agttgaagca taccttgaat cagttaacgc ttcggtagtt gagtttgctc
    15481 gctttgaagt tggtgaaggt atcgagaaag ctgcaaacga cttcgaagct gaagttgcag
    15541 ctacaatggc agcagccttg aataactaat aatagaaagg aagcaaattg cttccttttt
    15601 tgtatagggc ttagattttt cctgaaattg ttttttcccc agtctcactc gattagaata
    15661 attaaaacaa aaagccctat aataagggct aagtgcattt aggagactat acttcaaatt
    15721 catagtaagc gcgtcataac aaggtatcta tcctaattcc ccaacttcca gtatactaga
    15781 taaaaaataa ttatcggagg aaaggtattg tactatgata attcaactaa gtgatttagg
    15841 tcaagttcac cttgtttgtg gcaagacaga tatgaggcag ggaatagact cattagccta
    15901 tgtagttaaa acccactttg aattggattc tttctccggt caagtctttc tcttttgtgg
    15961 tggacgtaaa gaccgcttta aagcccttta ctgggatggt caaggatttt ggctactata
    16021 taaacgcttt gagaacggaa aactgacttg gctaagtaca gaaaaggatg tcaaagctct
    16081 cgcacctgaa caagtagact agcttatgaa gggcttttct atcactccaa aaatatagta
    16141 gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaatc gatttgactg
    16201 tcctgatcga tttgtcctgt tcttatttca ttttactata aatccatcag aaagtcgtga
    16261 tttctattga aatgaggact ttctttttac actcatctgc tttcaaaaag cattctagtc
    16321 cgtctccgat taacgatgga ctttatcacc tccttctcca gtccttgtat gacatcttcc
    16381 aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttgttca
    16441 atggggttca tctctggtgt gtatggagga ataaatgaaa agccaatatt agtcggaatc
    16501 tttaaggtac ttgatttatg ccatatagca ttgtccataa cgagtaaaag ataatcatct
    16561 ggataagctt gtgaaagctc ctattcctaa agccccttta gcgcataact ttggctcagc
    16621 ttctattatc gctcacacca tccatcagaa gtttaatctg aaggtaccca attatcgcca
    16681 agaagaaggt tgggctagga tgggtttacc aatcacacgt aaggaaattg ttaattggca
    16741 tatcaaggcg agtcaatact atttggagcc cctttataac ctcttgcgag agagactatt
    16801 gacgcagtcc ttacttcatg cggatgaaac ttcttatagg gtgctagaga gtgatagtca
    16861 gctgacttac tattggactt ttttgtctgg gaaagctgag aaacaaggga ttacgcttta
    16921 ccatcatgtc ctaatcgatt tgttcatatc ttatttcaat ccattataaa tagtgagaaa
    16981 tatctatcct atcttctaga atgtcttcca aacgtggaaa ctctcgtaaa caaagaggtt
    17041 ttagaggcct atttaccatg gactaaagtt gtacaagaaa agtgcaaata agaaatctcc
    17101 agattaggaa ctatccgtga gttctctagt ctggagattt ttcaatagac ttcgttattg
    17161 gatggttaca attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg
    17221 gcaaggactt ttttacctgt acctaatttt ttggcaacct caatggctcc gtagatagct
    17281 gcagctgagg aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca
    17341 agagcgtcat ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca
    17401 ggaataaatc cagctgagat accttgaatt ttgtgaggac cagatttttc accagataga
    17461 atagcagatt catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt
    17521 gcatgagaaa caccagaaat cgttccaccg gtacctactc cagcaacaaa ggcatctaat
    17581 ccatctttac cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga
    17641 ttagctggat tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga
    17701 gccttagcaa tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca
    17761 taagcttgga taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact
    17821 ttataccctt tagcagcacc tacccatgaa agtccaatac cagtgtttcc acttgttgct
    17881 tcaacaatag tagaaccagg tttcagaata ccatcttgtt cagctttttc aatcatgcta
    17941 agggcaatac ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg
    18001 tctgcagcac cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat
    18061 tcagtaatgt tgttataaat agacatatga atacctcttt gtataagata tctttagtat
    18121 aacgctatcc ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata
    18181 tatcatagat caaagtcatt aattttcaag tgattgcgtt cagctaattc tagtaaatag
    18241 gtgtgaagga aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg
    18301 tacgcatctt tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat
    18361 tattcattgt ttacactaag tttacaggaa cttcaacctc tcgtaaacct tggtcagtta
    18421 aagtgacttt tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt
    18481 tatcaacata aatcatcgta tcgacttgat ctgtaaagtt tgtatccagc tccatgagat
    18541 catgttcttt aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa
    18601 tgccagcctg ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc
    18661 cggcgtaagc acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca
    18721 cgaccacaca gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag
    18781 cagtaccact aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa
    18841 taaaggcaga gcagttatgt gtcgctttgt agtgttcttt tttgatggca gtaatgaagt
    18901 cacgagcctc ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga
    18961 tttcttcttg gacttgaccg tcctctttaa ttgttctaaa ttccatgatt taattgtact
    19021 aaaaaatcat ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt
    19081 atctcggtcg tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga
    19141 aacttccagc aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta
    19201 ttctagaaga atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga
    19261 agcgagtcag aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag
    19321 atgttctcaa atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc
    19381 ttcaagcagt agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga
    19441 cagaaatgat ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg
    19501 ctagtcctcg catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt
    19561 gcgggatagc tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg
    19621 caacaaccca tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag
    19681 tagatgcttt tccttatgtt gataatccaa tgctttacca cgctgtcaag aatagtgtaa
    19741 aggagaatgg attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg
    19801 tccgtttagg agaactaaaa agactgaatt taccgagacg gtttcatgga aatccgttga
    19861 ttattccaaa accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt
    19921 caccaaagtt aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt
    19981 ttgcttcaga aattaagaaa ggggagcagt tagaagaaat cttacaggag caatttccaa
    20041 atgagaaaat tggctttgta tcttctgtaa cagaggatcg attagagcaa gtacaagctt
    20101 ttcgagatgg agaactgaca atacttatca gtacgacaat cttggagcgt ggagttacct
    20161 tcccttgtgt ggatgttttc gtagtagagg ccaatcatcg tttgtttacc aagtctagtt
    20221 tgattcagat tggtggacga gttggacgaa gcatggatag accgacagga gatttgcttt
    20281 tcttccatga tgggttaaat gcttcaatca agaaggcgat taaggaaatt cagatgatga
    20341 ataaggaggc tggtctatga agtgcttgtt atgtgggcag actatgaaga ctgttttaac
    20401 ttttagtagt ctcttacttc tgaggaatga tgactcttgt ctttgttcag actgtgattc
    20461 tacttttgaa agaattgggg aagagaactg tccaaattgt atgaaaacag agttgtcaac
    20521 aaagtgtcaa gactgtcaac tttggtgtaa agaaggagtt ggagtcagtc atagagcgat
    20581 ttttacttac aatcaagcta tgaaggattt tttcagtcgg tataagtttg atggagactt
    20641 cctgttaaga aaagttttcg cttcattttt aagtgaggag ttgaaaaagt acaaagagta
    20701 tcaatttgtt gtaattcccc taagtcctga tagatatgct aatagaggat ttaatcaggt
    20761 tgagggcttg gtagaggcag caggctttga gtatctggat ttattagaga aaagagaaga
    20821 gagagccagt tcttctaaaa atcgttcaga gcgcttgggg acagaacttc ctttctttat
    20881 taaaagtgga gtcactattc ctaaaaaaat cctacttata gatgatatct atactacagg
    20941 agcaactata aatcgtgtta agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac
    21001 attttccctt gtaagatgag gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa
    21061 atcataaaaa caaaaatgtt tagaaagaag gtactcatat gattaaatat agtatccgtg
    21121 gtgaaaacct agaagtaaca gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga
    21181 tcgaaaagta cttccaacca gaacaagagt tggatgcccg aattaactta aaagtttatc
    21241 gtgaaaaaac ggctaaagtg gaagtaacga ttccgcttgg atctattact ctccgcgcag
    21301 aagatgtatc tcaagatatg tatggttcaa ttgaccttgt aactgataaa attgaacgtc
    21361 agattcgtaa aaataaaaca aaaatcgagc gtaaaaataa aaataaggta gcaactggtc
    21421 aattatttac agatgctttg gtggaagatt caaatattgt ccagtctaaa gttgttcgtt
    21481 caaaacaaat tgatttaaaa ccaatggatt tggaagaagc aattctacaa atggatttat
    21541 tggggcatga tttctttatc tatgtggatg ttgaagatca gacaaccaat gtgatttatc
    21601 gtcgtgagga tggcgaaatt ggtttgttag aggttaaaga atcttaattt caatatgtgt
    21661 aaaggtaggt ttactgaatt gtaaactacc tttttcttat aattgataga attactgatt
    21721 acacttttaa aaagtcgctt tttgaggggt attatggtat aatgggtgcc aagaggtttt
    21781 gaatgaaaaa attttatgta agtccaattt ttcctatcct agtaggattg attgcgtttg
    21841 gagtcttatc cactttcatt atttttgtta ataataatct gttgacggtt ttaattttgt
    21901 ttctttttgt aggaggctat gtttttttat ttaaaaaact gagagtgcat tatacaagga
    21961 gtgatgtaga acagatacag tatgtaaacc accaagcgga agaaagtttg acagctctat
    22021 tggaacagat gcctgtaggt gttatgaaat tgaatttatc ttctggagag gttgagtggt
    22081 ttaatcccta tgctgaattg attttgacca aggaagatgg tgattttgat ttagaagctg
    22141 ttcaaacgat tatcaaggct tcagtaggaa atccgtctac ttatgccaag cttggtgaga
    22201 agcgttatgc tgttcatatg gatgcttctt ccggtgtttt gtattttgta gatgtatcca
    22261 gggaacaagc cataacagat gaattggtaa caagtagacc agtgattggg attgtctctg
    22321 tggataatta tgatgatttg gaggatgcaa cttctgagtc agatattagt caaatcaata
    22381 gttttgtagc taattttata tcagagtttt cagaaaaata catgatgttt tctcgtcggg
    22441 taagtatgga tcgattttat ctatttactg actacacggt gcttgagggc ttgatgaatg
    22501 ataaattttc tgttattgat gctttcagag aagagtcgaa acagagacag ttgcccttga
    22561 ccttaagtat gggattttct tatggcgatg gaaatcatga tgagataggg aaagttgctt
    22621 tgctcaattt gaacttggct gaagtacgtg gtggcgacca ggtggttgtt aaggaaaacg
    22681 acgaaacgaa aaatccagtt tattttggtg gtgggtctgc tgcttcaatc aagcgtacac
    22741 ggactcgtac gcgcgctatg atgacagcta tttcagataa gattcggagt gtagatcagg
    22801 tttttgtagt cggtcacaaa aatttagaca tggatgcttt gggctctgct gtaggtatgc
    22861 agttgttcgc cagcaatgtg attgaaaata gctatgctct ttatgatgaa gaacaaatgt
    22921 ctccagatat tgaacgagct gtttcattca tagaaaaaga aggagttacg aagttgttgt
    22981 ctgttaagga tgcaatgggg atggtgacca atcgttcttt gttgattctt gtagaccatt
    23041 caaagacagc cttaacatta tcaaaagaat tttatgattt atttacccaa accattgtta
    23101 ttgaccacca tagaagggat caggattttc cagataatgc agtcattact tatatcgaaa
    23161 gtggtgcaag tagtgccagt gagttggtaa cggaattgat tcagttccag aattctaaga
    23221 aaaatcgttt gagtcgtatg caagcaagtg tcttgatggc tggtatgatg ttggatacta
    23281 aaaatttcac ctcgcgagta actagtcgga catttgatgt tgctagctat ctcagaacgc
    23341 gcggaagtga tagtattgct atccaggaaa tcgctgcgac agattttgaa gaatatcgtg
    23401 aggtcaatga acttatttta caggggcgta aattaggttc agatgtacta atagcagagg
    23461 ctaaggacac gaaatgctat gatacagttg ttattagtaa ggcagcagat gccatgttag
    23521 ccatgtcagg tattgaagcg agttttgttc ttgcgaagaa tacacaagga tttatctcta
    23581 tctcagctcg aagtcgtagt aaactgaatg tacaacggat tatggaagag ttaggcggtg
    23641 gaggccactt taatttggca gcagctcaaa ttaaagatgt aaccttgtca gaagcaggtg
    23701 aaaaactgac agaaattgta ttaaatgaaa taaaggaaaa ggagaaagaa gaatgaaagt
    23761 aatcttttta gcaaatgtta aaggaaaagg taaaaaaggc gaaattaagg aagtaccaac
    23821 agggtatgcg caaaactttc ttatcaaaaa gaatctagcc aaagaagcga ctgctcaagc
    23881 tgtaggtgaa cttcgtggta aacaaaaatc ggaagaaaaa gctcacgctg agatgattgc
    23941 agaaggaaaa gcaattaaag cacaacttga agcagaagaa actgttgtag aatttgttga
    24001 aaaagttggt ccagatggtc gtacctttgg ttctattacc aataagaaga ttgcagaaga
    24061 attgcaaaag caatttggaa ttaagattga taaacgtcat attcaagtac aagctccgat
    24121 tcgagcggtt ggtttgattg atgtgccagt gaaaatctat caagatatca caagtgtaat
    24181 caatcttcgt gtgaaagaag gataagttta caccttcttg acaagattgt aaaaggaagg
    24241 gaagtctgat ggcagaagta gaagagttac gagtacaacc tcaagatatc ttagctgagc
    24301 aatccgtttt aggggctatc tttattgatg agagtaaact tgtttttgtg cgagaataca
    24361 ttgagtctcg ggactttttt aagtatgccc atcgtttgat tttccaagcc atggtcgatt
    24421 tatccgatcg tggtgatgcc atagatgcaa caacggttcg tactatcctt gataatcaag
    24481 gtgatttaca gaatattggt ggcttgtctt acttggttga gattgttaat tctgtgccaa
    24541 cttctgctaa tgcggagtat tatgctaaga ttgttgcaga aaaagcaatg ctacgtcgtt
    24601 taattgccaa gttgacagag tctgtcaacc aagcttacga agcgtcacaa ccagctgatg
    24661 aaattattgc tcaggcagaa aaagggctga ttgatgtcag tgaaaatgca aatcgaagcg
    24721 ggtttaagaa cattcgagat gtgttgaatc tcaactttgg aaatctggaa gctcgctcgc
    24781 aacaaacgac cgatattaca ggtattgcga caggttatcg tgatttggat catatgacaa
    24841 caggacttca tgaggaggag ttgattatct tagcagctcg tccagcagtt ggtaagacag
    24901 catttgcctt gaatatcgct cagaacattg ggactaagtt ggacaaaacg gttgctattt
    24961 tttcactcga aatgggtgcg gaaagcttgg tagatcgtat gttagctgca gaaggcttag
    25021 tggagtcaca ttctatccgt acagggcaat tgacagatga ggagtggcaa aaatatacta
    25081 ttgctcaggg taatctagct aacgccagta tctatatcga tgatacgcca ggtattcgga
    25141 ttacagagat tcgttctcgt tctcgtaaat tggctcaaga aactggaaat cttggtttga
    25201 ttttgataga ctatttgcaa cttatcacgg gaactggtcg agaaaatcgt caacaagaag
    25261 tttctgaaat ttctcgtcag ttgaaaatac tagccaagga attgaaggtt ccagtaatcg
    25321 ctctgagtca gctttctcgt ggtgtagaac aacgtcagga caagagaccg gtcttgtctg
    25381 atattcgtga atctgggtct attgagcagg acgctgatat cgtagctttt ctctatcgcg
    25441 atgactacta tgaacgtggt ggtgaagaag aggagggtat cccaaataat aaggtggaag
    25501 ttattatcga gaaaaaccgt agtggagctc gtggaacagt ggaattgatt ttccaaaaag
    25561 aatacaataa attttcaagt atctcaaaga gggaggcata agatgtcaga tgcatttaca
    25621 gatgtagcca agatgaaaaa aatcaaagaa gaaatcaagg cacatgaggg acaagtcgta
    25681 gaaatgactt tggagaatgg tcgtaagcgc caaaaaaata gattgggtaa gctaattgaa
    25741 gtttatccat ctctatttat tgtggagttt ggggatgtgg aaggagataa acaagttaat
    25801 gtttacgttg aatcctttac ttactcagat attcttacag aaaagaattt gattcattat
    25861 cttgactaaa gtgagaaatt ttctcacttt ttcttttttc tccgaataat ttaggtgaag
    25921 gcaatcatcg ctttatatta tttttcaagg aggaagaatg aaaattttac cgtttatagc
    25981 aagaggaaca agttattact tgaagatgtc agttaaaaag cttgttcctt ttttagtagt
    26041 aggattgatg ctagcagctg gtgatagtgt ctatgcctat tccagaggaa atggatcgat
    26101 tgcgcgtggg gatgattatc ctgcttatta taaaaatggg agccaggaga ttgatcagtg
    26161 gcgcatgtat tctcgtcagt gtacttcttt tgtagccttt cgtttgagta atgtcaatgg
    26221 ttttgaaatt ccggcagctt atggaaatgc gaatgaatgg ggacatcgtg ctcgtcggga
    26281 aggttatcgt gtagataata caccgacgat tggttccatt acttggtcta ctgcaggaac
    26341 ttatggtcat gttgcctggg tgtcaaatgt aatgggagat cagattgaga ttgaggaata
    26401 taactatggt tatacagaat cctataataa acgagttata aaagcaaaca cgatgacagg
    26461 atttattcat tttaaagatt tggatggcgg cagcgttggg aatagtcaat cctcagcttc
    26521 aacaggcgga actcattatt ttaagaccaa gtctgctatt aaaactgaac ccctagctag
    26581 cggaactgtg attgattact attatcctgg ggagaaggtt cattatgatc agatacttga
    26641 aaaagacggc tataagtggt tgagttatac tgcctataat ggaagctatc gttatgttca
    26701 attggaggct gtgaataaaa atcctctagg tcattctgtt ctttcttcaa caggcggaac
    26761 tcattatttt aagaccaagt ctgctatcaa aactgaaccc ctagttagtg caactgtgat
    26821 tgattactat tatcctggag agaaggttca ttatgatcaa attctcgaaa aagacggcta
    26881 caagtggttg agttatacgg cttataacgg aagtcgtcgc tatatacagc tagagggagt
    26941 gacttcttca caaaattatc agaatcaatc aggaaacatc tctagctatg gatcccatag
    27001 tagttcaact gtcggttgga agaaaataaa tggtagttgg tatcatttca aatcaaatgg
    27061 ttctaaatca acaggatggc tgaaagacgg ttctagctgg tattatttga aattatctgg
    27121 tgaaatgcag acaggatggt taaaggaaaa tggtttgtgg tattatctgg gtagttcagg
    27181 ggcaatgaaa acaggctggt accaggtctc tggtaagtgg tattattctt actcttcagg
    27241 cgccttagct gttaatacga cggtggatgg ctacagagta aacagtgatg gagaacgagt
    27301 atagaaaatt ggagtaggag aaatttcctg ctcttttctg taagcagttt ccttgacatt
    27361 tttcttattt tgcgctatca tatatacata taatatatgg gagtctgtgt acagtctgag
    27421 aggaagtgtt aaacttcgac cgcacctgat ctgggtaatg ccagcggagg gaacgatact
    27481 tagtctaatt ttgcaccttt tccatgtatg gtaaaggttt ttcttttttt aaaaaggaaa
    27541 acgagaagag gaggttctta tgaaagcaag cattgccttg caagttttac ccctagtaca
    27601 ggggattgat cggatagctg ttattgatca ggtcattgct tatctgcaaa ctcaagaagt
    27661 gacgatggta gtgacaccat ttgaaacggt cttggaaggg gagtttgatg agcttatgcg
    27721 cattctaaaa gaagcgctgg aagtggcagg gcaggaggca gacaatgtct ttgccaatgt
    27781 caaaataaat gtaggagaga ttttaagtat tgatgagaaa cttgagaagt atactgagac
    27841 gacacattag tctattgggc tttctcggag tattgtcaat ctggcagtta gcaggttttc
    27901 ttaaacttct ccccaagttt atcctgccga cacctcttga aattctccag ccctttgttc
    27961 gtgacagaga atttctctgg caccatagct gggcgacctt gagagtggct ttactggggc
    28021 tgattttggg agttttgatt gcctgtctta tggctgtgct catggatagt ttgacttggc
    28081 tcaatgacct gatttaccct atgatggtgg tcattcagac cattccgacc attgccatag
    28141 ctcctatcct ggtcttgtgg ctaggttatg ggattttgcc caagattgtc ttgattatct
    28201 taacgacaac ctttcccatc atcgttagta ttttggacgg ttttaggcat tgtgacaagg
    28261 atatgctgac cttgtttagt ctgatgcggg ctaagccttg gcaaatcctg tggcatttta
    28321 aaatcccggt tagcctgcct tacttttatg caggtctgag ggtcagtgtc tcctacgcct
    28381 ttatcacaac tgtggtatct gagtggttgg gaggttttga aggtcttggt gtttatatga
    28441 ttcagtctaa aaaactgttt cagtatgata ccatgtttgc cattattatt ctggtgtcga
    28501 ttatcagtct tttgggtatg aagctggtcg atatcagtga aaaatatgtg attaaatgga
    28561 aacgtactta aaatagagcg tttttgaaaa agaaaagagg aaatcaaaat gaagaaaaca
    28621 tggaaagtgt ttttaacgct tgtaatagct cttgtagctg ttgtgcttgt ggcctgtggt
    28681 caaggaactg cttctaaaga caacaaagag gcagaactta agaaggttga ctttatccta
    28741 gactggacac caaataccaa ccacacaggg ctttatgttg ccaaggaaaa aggttatttc
    28801 aaagaagctg gagtggatgt tgatttgaag ttgccaccag aagaaagttc ttctgacttg
    28861 gttatcaacg gaaaggcacc atttgcagtg tatttccaag actacatggc taagaaattg
    28921 gaaaaaggag caggaatcac tgccgttgca gctattgttg aacacaatac atcaggaatc
    28981 atctctcgta aatctgataa tgtaagcagt ccaaaagact tggttggtaa gaaatatggg
    29041 acatggaatg acccaactga acttgctatg ttgaaaacct tggtagaatc tcaaggtgga
    29101 gactttgaga aggttgaaaa agtaccaaat aacgactcaa actcaatcac accgattgcc
    29161 aatggcgtct ttgatactgc ttggatttac tacggttggg atggtatcct tgctaaatct
    29221 caaggtgtag atgctaactt catgtacttg aaagactatg tcaaggagtt tgactactat
    29281 tcaccagtta tcatcgcaaa caacgactat ctgaaagata acaaagaaga agctcgcaaa
    29341 gtcatccaag ccatcaaaaa aggctaccaa tatgccatgg aacatccaga agaagctgca
    29401 gatattctca tcaagaatgc acctgaactc aaggaaaaac gtgactttgt catcgaatct
    29461 caaaaatact tgtcaaaaga atacgcaagc gacaaggaaa aatggggtca atttgacgca
    29521 gctcgctgga atgctttcta caaatgggat aaagaaaatg gtatccttaa agaagacttg
    29581 acagacaaag gcttcaccaa cgaatttgtg aaataatgac agaaattaga ctagagcacg
    29641 tcagttatgc ctatggtcag gagaggattt tagaggatat caacctacag gtgacttcag
    29701 gcgaagtggt ttccatccta ggcccaagtg gtgttggaaa gaccaccctc tttaatctaa
    29761 tcgctgggat tttagaagtt cagtcaggga gaattgtcct tgatggtgaa gaaaatccca
    29821 aggggcgcgt gagttatatg ttgcaaaagg atctgctctt ggagcacaag acggtgcttg
    29881 gaaatatcat tctgcccctc ttgattcaaa aggtggataa ggcagaagct atttcccgag
    29941 cggataaaat tcttgcgacc ttccagctga cagctgtaag agacaagtat cctcatgaac
    30001 ttagcggtgg gatgcgccag cgtgtagcct tactccggac ctaccttttt gggcacaagc
    30061 tctttctctt agatgaggcc tttagcgcct tggatgagat gacaaagatg gaactccacg
    30121 cttggtatct tgagattcac aagcagttgc agctaacaac cctgatcatc acgcatagta
    30181 ttgaggaggc cctcaatctc agcgaccgca tctatatctt gaaaaatcgc cctgggcaga
    30241 ttgtttcaga aattaaacta gattggtctg aagatgagga caaggaagtc caaaagattg
    30301 cctacaaacg tcaaatcttg gcagaattag gcttagataa gtagaaaaat agggagttgg
    30361 tgaagattat cctttaccag cgcccttttt cttttaaaaa tgagaaaatt tcggtataat
    30421 agtcaaacaa ggtcaaggtt taaagagaga ggtgggtttg ttatgagatt taaaaataca
    30481 tcggatcata ttgaggccta catcaaggcg attttagatc aatctggtat cgtggagttg
    30541 caacggagtc agttggcaga tacctttcag gttgttccta gtcagattaa ctacgtgatc
    30601 aagacacgct ttacggaaag tagaggctac ttggttgaaa gtaagcgtgg tggtggaggc
    30661 tacattcgta taggacgaat tgagttttct agtcatcatg aaatgctccg ggagctgctt
    30721 tactcgattg gtgagcgagt cagtcaagaa atttatgagg atattctcca gcttttggtt
    30781 gagcaggaat tgatgaccaa gcaggagatg aatttgctag aatcagtagc tttggatcgc
    30841 gttttaggag aagaagctcc agttgttcga gcaaacatgc tacgtcagat catacaagag
    30901 gtagatagaa aagggaagta agatgaacta ttcaaaagca ttgaatgaat gtatcgaaag
    30961 tgcctacatg gttgctggac attttggagc tcgttatcta gagtcttggc acttgttgat
    31021 tgccatgtct aatcacagtt atagtgtagc aggggcaact ttaaatgatt atccgtatga
    31081 gatggaccgt ttagaagagg tggctttgga actgactgaa acggactata gccaggatga
    31141 aacttttacg gaattgccgt tctcccgtcg tttgcagatt ctttttgacg aagcagagta
    31201 tgtagcgtca gtggtccatg ctaaggtgct aggtacagag cacgtcctct atgcgatttt
    31261 gcatgatggc aatgccttgg cgactcgtat cttggagagg gctggttttt cttatgaaga
    31321 caagaaagat caggtcaaga ttgctgctct tcgtcgaaat ttagaagaac gggcaggctg
    31381 gactcgtgaa gatctcaagg ctttacgcca acgccatcgt acagtagctg acaagcaaaa
    31441 ttctatggcc aatatgatgg gcatgccgca gactccgagt ggtggtctcg aggattatac
    31501 gcatgatttg acagagcaag cgcgttctgg caagttagaa ccagtcatcg gtcgggacaa
    31561 ggaaatctca cgtatgattc aaatcttgag ccgtaagact aagaacaacc ctgtcttggt
    31621 tggggatgct ggtgtcggga aaacagctct ggcccttggt cttgcccagc gtattgctag
    31681 tggtgacgtg cctgcggaaa tggctaagat gcgcgtgtta gaacttgatt tgatgaatgt
    31741 cgttgcaggg acacgcttcc gtggtgactt tgaagaacgc atgaataata tcatcaagga
    31801 tattgaagaa gatggccaag tcatcctctt tatcgatgaa ctccacacca tcatgggttc
    31861 tggtagcggg attgattcga ctctggatgc ggccaatatc ttgaaaccag ccttggcgcg
    31921 tggaactttg agaacggttg gtgccaccac tcaggaagaa tatcaaaaac atatcgaaaa
    31981 agatgcggca ctttctcgtc gtttcgctaa agtgacgatt gaagaaccaa gtgtggcaga
    32041 tagtatgact attttacaag gtttgaaggc gacttatgag aaacatcacc gtgtacaaat
    32101 cacagatgaa gcggttgaaa cagcggttaa gatggctcat cgttatttaa ccagtcgtca
    32161 cttgccagac tctgctatcg atctcttgga tgaggcggca gcaacagtgc aaaataaatc
    32221 aaagcatgta aaagcagacg attctgactt gagtccagct gacaaggcct tgatggatgg
    32281 caagtggaaa caggcagccc agctaatcgc aaaagaagag gaagtgcctg tctataaaga
    32341 cttggtgaca gagtctgata ttttgaccat cttgagtcgc ttgtcaggta tccccgttca
    32401 aaaactgact cagactgatg ccaagaaata cttaaacttg gaagctgaac tgcacaaacg
    32461 tgtcatcggt caagaccaag ctgtttcaag tattagccgt gccattcgcc gcaatcagtc
    32521 aggaattcgc agtcacaagc gtccgatcgg ttcctttatg ttcctaggac ccacaggtgt
    32581 cggtaagacc gagttggcca aggctttggc agaagttctc tttgatgacg aatcagccct
    32641 tatccgcttt gatatgagtg agtatatgga gaaattcgca gccagccgtc taaatggagc
    32701 tcctccaggc tatgtgggtt acgaagaagg tggggagttg acagagaagg ttcgcaataa
    32761 accctattcc gttctcctct ttgatgaggt agagaaggcc catccagata tctttaatgt
    32821 tctcttgcag gttctggatg acggtgtctt gacagatagc aagggacgca aggtcgattt
    32881 ttcaaatacc attatcatta tgacatcgaa cttaggtgcg actgcccttc gtgatgataa
    32941 gactgttggt tttggggcta aggatattcg ttttgaccag gaaaatatgg aaaaacgcat
    33001 gtttgaagaa ctgaaaaaag cttatagacc ggaattcatc aaccgtattg atgagaaggt
    33061 ggtcttccat agcctatcta gtgaccatat gcaggaagtg gtgaagatta tggtcaagcc
    33121 tttagtggca agtttggctg aaaaaggcat tgacttgaaa ttacaagctt cagctctgaa
    33181 attgttagca aatcaaggat atgacccaga gatgggagct cgcccacttc gcagaaccct
    33241 gcaaacagaa gtggaggaca agttggcaga acttcttctc aagggagatt tagtggcagg
    33301 cagcacactt aagattggtg tcaaagcagg ccagttaaaa tttgatattg cataaaagaa
    33361 taaaagtacc agcatctgac cataagtcac agtggagtga aactcaatga aaatcaaaga
    33421 gcaaactagg cagctagccg caggttgctc aaaacactgg tttgaggttg tagatagagc
    33481 tgacgtggtt tgaagagatt ttcgaagagt ataagctgca agatgaatga ttttcttgta
    33541 ttgacgttgt tgttgacaaa aagtagcgga taaatgaaat ccattccatt atcatagatg
    33601 ataggctggt aggaaatttt caaatagcat acagaaaata gatgtatgga gttctggtag
    33661 tagaaaggga gagagatgaa cattttagtt gcagatgacg aggaaatgat tagagaagga
    33721 attgcagcat ttctgacaga agagggttat catgtcatta tggctaagga tggacaagag
    33781 gtcttggaaa aatttcaaga tctccctatc catctcatgg tactggattt aatgatgcct
    33841 aggaagagtg gttttgaagt gttaaaagaa atcaatcaaa agcacgatat tcctgtcatc
    33901 gtcttgagtg ctctgggaga tgaaactact cagtcacagg tatttgatct ctatgctgat
    33961 gatcatgtga caaaaccttt ttctttggta ctgcttgtca agcgtattaa ggcgcttatc
    34021 agacgttact acgtcataga ggatctttgg cgatatcagg atgtaacagt ggattttacc
    34081 tcttacaaag cacattataa aaatgaagaa attgatctca aaccaaagga attactggta
    34141 ctaaagtgtt tgattcagca taaaaatcaa gttttaagta gagagcagat attggaagaa
    34201 atttcaaaag atgtagctga tttaccttgt gatagggtcg ttgatgtcta tattcgtact
    34261 cttcgcaaaa aattagcttt agattgtatc gtgactgtga aaaatgttgg gtataagatt
    34321 agcttatgat aaaaaatcct aaattattaa ccaagtcttt tttaagaagt tttgcaattc
    34381 taggtggtgt tggtctagtc attcatatag ctatttattt gacctttcct ttttattata
    34441 ttcaactgga gggggaaaag tttaatgaga gcgcaagagt gtttacggag tatttaaaga
    34501 ctaagacatc tgatgaaatt ccaagcttac tccagtctta ttcaaagtcc ttgaccatat
    34561 ctgctcacct taaaagagat attgtagata agcggctccc tcttgtgcat gacttggata
    34621 ttaaagatgg aaagctatca aattatatcg tgatgttaga tatgtctgtt agtacagcag
    34681 atggtaaaca ggtaaccgtg caatttgttc acggggtaga tgtctacaaa gaagcaaaga
    34741 atattttgct tttgtatctc ccatatacat ttttggttac aattgctttt tcctttgttt
    34801 tttcttattt ttatactaaa cgcttgctca atcctctttt ttacatttca gaagtgacta
    34861 gtaaaatgca agatttggat gacaatattc gttttgatga aagtaggaaa gatgaagttg
    34921 gtgaagttgg tgaagttgga aaacagatta atggtatgta tgagcacttg ttgaaggtta
    34981 ttcatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt gcaaaatcaa aaggtttcct
    35041 ttgtccgcgg agcatcacat gagttgaaaa cccctttagc cagtcttaga attatcctag
    35101 agaatatgca gcataatatt ggagattaca aagatcatcc aaaatatatt gcaaagagta
    35161 taaataagat tgaccagatg agccacttat tggaagaagt actggagtct tctaaattcc
    35221 aagagtggac agagtgtcgt gagaccttga ctgttaagcc agttttagta gatattttat
    35281 cacgttatca agaattagct cattcaatag gtgttacaat tgaaaatcaa ttgacagatg
    35341 ctaccagggt cgtcatgagt cttagggcat tggataaggt tttgacaaac ctgattagta
    35401 atgcaattaa atattcagat aaaaatgggc gcgtaatcat atccgagcaa gatggctatc
    35461 tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga actagaacat ttatttgata
    35521 tattctatca ttctcaaatc gtgacagata aggatgaaag ttccggtttg ggtctttaca
    35581 ttgtgaataa tattttagaa agctatcaaa tggactatag ttttctccct tatgaacacg
    35641 gtatggaatt taagattagc ttgtagacag attagttttt tattaaagtt catatagggt
    35701 taacataagt gtgttattct ttgtgtagat aaaagaaagg atactaatat ggtattagcg
    35761 attattttag taacattcct tattcgattg atttttttaa agcgttcgat agagaatgag
    35821 aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa acgattaact
    35881 atggctcata ttgtttttta tctctcttgc ttggttgagg caatggtgca caagacaatt
    35941 tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct gatgttgatg
    36001 ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt caataatcac
    36061 aaatatgtag atcatatctt gtttaggaca gtaaaacatc ctaattactt tttaaatatt
    36121 cttcctgagt tgattggctt gaccttgttg agtcatgctt atgtgacttt tgttttagtt
    36181 tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa gctattacat
    36241 gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgat ttatatacag
    36301 ttcatattga agtgatatag taaggttaaa gaaaaaatat aggagataat tatgtttgca
    36361 tcaaaaagcg aaagaaaagt acattattca attcgtaaat ttagtattgg agtagctagt
    36421 gtagctgttg ccagtctttt tatgggaagt gtggttcatg cgacagagaa ggaggtaact
    36481 acccaagtag ccacttcttc taataaggca aataaaagtc agacagaaca tatgaaagct
    36541 gctgaacaag gaaaacaaca tagagaaatc gatttagacc gaaataaggc aaagaaagcg
    36601 atcgatgaat atatagaaaa aatgttgagt gagatccaat tagataaaag aaaacatacc
    36661 caaaatttcg ccttaaacat aaagttgagc agaattaaaa cggagtattt gtatggatta
    36721 agtgttttaa aagagaagtc ggaagctgag ttgccgtcaa aagctgagtt gccgtcaaaa
    36781 ataaaagaaa agttaaccgc agcttttgag cagtttaaaa aagatacatt gaaaccagga
    36841 gaaaaggtag cagaagctaa gaagaaggtt gaagaagctg agaaaaaagc caaggatcaa
    36901 aaagaagaag atcgtcgtaa ctacccaacc attacttaca aaacgcttga acttgaaatt
    36961 gctgagtccg atgtggaagt taaaaaagcg gagcttgaac tagtaaaaga ggaagctaac
    37021 aaacctcgaa acgagcaaaa agttaagcaa gcaaaagcga aagttgagag taaacaagct
    37081 gaggctacaa ggttaaaaaa aatcaagaca gatcgtgaag aagcagaaga agaagctaaa
    37141 cgaagagcag aagctaagtt gaaggaagct gttgaaaaga atacagcgac ttcagagcaa
    37201 ggtaaaccaa agaggcgggg aaaacgagga gctcttggag agcaagcaac acctgataaa
    37261 aaagaaaatg atgcgaagtc ttcagattct agcgtaggtg aagaaactct tccaagccca
    37321 tccctgaaac cagaaaaaaa ggtagcagaa gctcagaaga aggttgcaga agctgagaaa
    37381 aaagccaagg atcaaaaaga agaagatcgc cgtaactacc caaccaatac ttacaaaacg
    37441 cttgaacttg aaattgctga gtccgatgtg aaagttaaag aagcggagct tgaactagta
    37501 aaagaggaag ctaaggaatc tcgaaacgag gaaaaaatta agcaagtaaa agcgaaagtt
    37561 gagagtaaaa aagctgaggc tacaaggcta gaaaacatca agacagatcg taaaaaagca
    37621 gaagaagaag ctaaacgaaa agcagcagaa gaagataaag ttaaagaaaa accagctgaa
    37681 caaccacaac cagcgccggc tcctcaacca gaaaaaccaa ctgaagagcc tgagaatcca
    37741 gctccagctc caaaaccaga gaagccagct gaacaaccaa aagcagaaaa aacagatgat
    37801 caacaagctg aagaagacta tgctcgtaga tcagaagaag aatataatcg cttgactcaa
    37861 cagcaaccgc caaaaattga aaaaccagct ccagcaccac aaccagagca accagctcca
    37921 gcaccaaaaa caggctggaa acaagaaaac ggtatgtggt acttctacaa tactgatggt
    37981 tcaatggcaa caggatggct ccaaaacaat ggttcatggt actacctcaa cagcaatggc
    38041 gctatggcga caggatggct ccaaaacaat gattcatggt actacctcaa cgctaatggt
    38101 gatatggcga caggatggct ccaatacaat ggttcatggt actacctcaa cgctaatggt
    38161 gatatggcga caggatggtt ccaaaacaat ggttcatggt actacctcaa cgctaatggt
    38221 gatatggcga caggatggct ccaatacaat ggttcatggt actacctaaa cagcaatggt
    38281 gctatggtaa caggatggct ccaaaacaat ggctcatggt actacctcaa cgctaacggt
    38341 tcaatggcaa cagattgggt gaaagatgga gatacctggt actatcttga agcatcaggt
    38401 gctatgaaag caagccaatg gttcaaagta tcagataaat ggtactatgt caatggctca
    38461 ggtgcccttg cagtcaacac aactgtagat agctatagag tcaatgccaa tggtgaatgg
    38521 gtaaactaaa cctaatataa ctagttaata ctgacttcct gtaagaactc tttaaagtat
    38581 tccctacaaa taccatatcc tttcagtaga taatataccc ttgtaggaag tttagattaa
    38641 aaaataactc tgtaatctct agccggattt atagcgctag agactacgga gtttttttga
    38701 tgaggaaaga atggcggcat tcaagaggct ctttaagaga gttacgggtt ttaaactatt
    38761 aagccttctc caattgcaag agggcttcaa tctctgctag ggtgctagct tgcgaaatgg
    38821 ctccacggag tttggcagcg ccagatgttc cacggagata gtgaggagcg aggccgcgga
    38881 attcacgaac tgcgacgttt tctcctttga ggttaatcaa tcgtttcaag tgttcgtagg
    38941 cgatcttcat cttgtcttca aaggtcaaat caggtaggat ttctcctgtt tcaaagtaat
    39001 ggttgatttg gttgaagagg taaggatttc ccatggcagc tcggccaatc atgactgcgt
    39061 cagcaccaac ttcttcgatg cgttgcttgg cttcttggac agtacggata tcaccgttgg
    39121 cgatgaatgg aatcttggtt agagcttggg caaccttgta aagggtctca aggtctgcgt
    39181 ggccagtata catttgttca cgggtacggc catgcatggc gagggcagaa acacctgcag
    39241 cctcagcagc gagggcattt tctactgcca gagatgggtc cgcccagcca gtacgcattt
    39301 tgacagtaag tgggatatca aggacagact ggaccttgtt gatgatggag taaatcttgt
    39361 ctggatcctt gagccacata gcaccagctt cgttcttcac gattttgttg acagggcagc
    39421 ccatgttgat atcgacgata tcggtcttgg tgttttcttg gatgaattct gctgcgcgtg
    39481 ctaggctgtc ttcatcgcta ccaaaaagtt ggatagagac agggttttcg ccctcatcga
    39541 tatgaagcat gtgcagggtt ttttcgttgt tgtattggat tcccttgtca gagaccattt
    39601 ccattacaac gagtccagct ccgagctcct ttgcgatggt acgaaaggct gagttggtca
    39661 cgccagccat aggcgctaaa acggtacgat tgggaatctc aacattgcca atcataaaag
    39721 gtgtattaag atttgtcacg aatgagttcc tccaggtcct tttcatcaaa gttgtaagta
    39781 gtttggcaga attgacaagt gatttctgcc ccgtggtctt cctctttcat ttcctgtaag
    39841 tctgagcttg gaaggctggc aagagcgttc ataaagcgtt catggctaca gtcacattgg
    39901 aaacggattt cttcttcaga aagacgcttg taggcttcgt ctccgtagat agccttgagg
    39961 agggcttcga tatggtcgtc gctttcgaga agagtagaga tagctggcat ttcttggatg
    40021 cgtttttcaa agcgagcaat ctcttctttc ttggctcctg gcaagacttg aactaggaaa
    40081 ccacctgcaa ccttgacctt gtcttcctcg tccaaaagga cattgaggcc gaccgctgaa
    40141 ggcgtttgtt ggctttcagt aaggtaaaag gcaaggtctt caccgatttc tccagagatg
    40201 aggggagtta tagaattgta aggatttcca gtaccgtagt ctgtgataac gaggaattga
    40261 ccatttccaa caaaaggtcc gactaggact tcaccagtcg cagtcttttt gatgtcaaca
    40321 ccaggatttt gaacgtagcc tttgacgttc cccttggtat cagcgacggt gataatagca
    40381 cctagagagc tagatcccaa caccttaact gtaagtttgg tatttccttt ttcattggct
    40441 gcgagaatct ggctagcgat aagagttcga ccaagcgcta cagttgagct agcttgggtt
    40501 tgatgttttt cttgagcagt gcggacggtt tccgtgctat caaggacaaa agcacgaaag
    40561 gctccgcttt ctgatatagt tttaataatt ttatccatag ctactatttt agcataaaaa
    40621 tgcccaaagg gggagccgtg tgtttactga ttttcaggat aatggaccag gaaatcagca
    40681 tgaaaataaa aagagaaaca gattatttca gcatttgtaa gatttatgct atgcttaagg
    40741 tagaaaatga aaggggtaac aaatgtattt aggagatttg atggagaaag ccgagtgtgg
    40801 tcaattttca atcctttcct ttctattaca agagtctcag acgaccgtca aggctgtaat
    40861 ggaagaaaca ggattttcaa aagcaaccct aaccaaatat gtcaccctgc tcaatgacaa
    40921 ggctttggat agtggcttag agctgactat tcactcagaa gatgaaaatc tgcgtctgtc
    40981 tatcggtgca gctaccaagg ggagagatat tcggagcttg tttttggata gtgctgttaa
    41041 ataccagatt ttggtttatc ttctctacca ccaacagttt ttagcccatc agctggctca
    41101 agaattggtg attagcgagg ctacgcttgg tcgtcacttg gctggtttaa atcagatttt
    41161 gtcagaattt gatttatcca tccaaaatgg acgttggcga ggtccagagc atcagattcg
    41221 ctatttctat ttctgtcttt tccgaaaggt ctggtcgagt caggaatggg aaggtcacat
    41281 gcagaaacca gagagaaaac aggagattgc caatttagag gaaatctgcg gtgcaagttt
    41341 gtctgcgggg cagaaattgg acttggttct ctgggctcac atcagtcaac aacgtcttcg
    41401 ggtcaatgct tgtcagtttc aagttataga agagaaaatg cgagggtatt ttgacaatat
    41461 cttctatctt cgtttgctga gaaaggttcc gtcctttttt gctgggcaac atattccact
    41521 aggagttgag gatggtgaga tgatgatatt cttctctttt ctcctatctc atcgcattct
    41581 tcctcttcat actatggagt atattcttgg ttttggaggg cagttggcag atttactgac
    41641 gcaattgatt caagaaatga agaaggagga actattgggg gattatacag aggaccatgt
    41701 cacctatgaa ctcagtcagc tttgtgctca agtctatctc tataagggct atattttaca
    41761 ggatcgctac aagtaccagt tagagaatcg tcatccatat ttactgatgg aacatgattt
    41821 taaagagaca gcagaggaga tttttcatgc tctacctgct tttcaacagg ggacagattt
    41881 agataagaag attctctggg aatggctcca gttaatcgaa tatatggctg aaaacggtgg
    41941 ccagcatatg cggattggtc tggatttgac atctggtttt cttgtctttt caaggatggc
    42001 agccattttg aaacggtatt tggaatacaa tcgttttatt accattgaag cttatgaccc
    42061 tagtcggcat tatgatttgc tggttaccaa taacccgatt cataagaagg aacagacacc
    42121 agtctattat ttaaaaaatg acttggatat ggaggatttg gcagggattc gccagttatt
    42181 attcacttaa aaggcttggt taatccaggt cttttttgtg aaattcacac aatctcctca
    42241 cattttttta aaaattaaaa aaagttgaca aacaagaaag cgctttattt tgtatactag
    42301 taagtgtaaa gaggaaacac ctcaagatct ttatcaggag gacagtacat gtcacaagaa
    42361 aaatacatca tggccattga ccagggaact acaagttctc gtgccatcat tttcaacaaa
    42421 aaaggggaaa aggttagctc gagtcaaaaa gagtttaccc agattttccc tcaggcaggt
    42481 tgggttgagc acaatgccaa tgaaatttgg aactctgttc agtcagttat tgcgggtgct
    42541 ttcatcgaaa gtggtgtcaa gccaaatcaa atcgaggcaa tcgggattac caaccaacgt
    42601 gaaacaacgg ttgtctggga taagaaaaca ggacttccta tctacaatgc tatcgtttgg
    42661 cagtcacgcc agacagcacc tttggctgag caactaaaaa gccaaggtta tgtggaaaaa
    42721 ttccatgaaa agactggttt gattattgat gcttacttct ctgctaccaa ggttcgttgg
    42781 attttggatc atgtagaaga tgctcaagag cgagcagaaa aaggggaatt gctctttggt
    42841 actatcgata cttggttggt ttggaaattg actgacggtg cggctcacgt gactgactac
    42901 tcaaatgcag ctcgtaccat gctttataac attaaagaac tcaaatggga tgatgagatt
    42961 ttggaaatcc ttaacattcc gaaggctata cttccagaag ttcgttctaa ctccgaaatc
    43021 tacggcaaga cagctccatt ccatttctac ggtggagagg tgccaatctc aggtatggct
    43081 ggggaccaac aagcagccct ctttggacag ttggcttttg agccaggtat ggttaagaat
    43141 acttatggaa caggctcttt catcatcatg aatactgggg aagagatgca gttgtctgaa
    43201 aacaacctct tgacaaccat tggttacgga atcaacggta aggtttatta tgccttggaa
    43261 ggttctatct tcatcgcagg aagtgctatt cagtggcttc gtgacggtct tcgcatggtt
    43321 gaaaattcac cagaatctga aaaatacgct cgtgattctc acaacaacga tgaagtttat
    43381 gtcgttccag cctttacagg tctaggcgct ccatactgga accaaaatgc tcgtggttcc
    43441 gtctttggtt tgactcgtgg aacaagcaaa gaagacttta tcaaggcgac tttgcaatct
    43501 attgcttatc aagtgcgtga catcatcgac actatgcaag tggatactca gactgccatt
    43561 caagtactga aggtggatgg tggtgcagcc atgaacaact tcctcatgca gttccaggcg
    43621 gatattttag gcattgacat tgcacgtgct aaaaacttgg aaacaacagc tctaggagcg
    43681 gccttcctag caggtttgtc agtagggtac tggaaagact tggacgagtt gaaactcttg
    43741 aacgagacag gagaactctt tgagccatct atgaatgaat ctcgcaagga acaactctac
    43801 aagggctgga agaaggctgt gaaagcaact caagtctttg cggaagtaga cgactaatac
    43861 tggcagaata aagcgattta tttagaaagt gtgtaaatat ggaattttca aagaaaacac
    43921 gtgaattgtc aattaaaaaa atgcaggaac gcaccctgga cctcttgatt atcggtggag
    43981 gaatcacagg agctggtgta gccttgcagg cggcagctag cggtcttgag actggtttga
    44041 ttgaaatgca agactttgca gaaggaacat ctagtcgttc aacaaaattg gttcacggag
    44101 gacttcgtta cctcaaacaa tttgacgtag aagtggtctc agatacggtt tctgaacgtg
    44161 cagtggttca acaaatcgct ccacacattc caaaaccaga tccaatgctc ttaccagttt
    44221 acgatgaaga tggagcaacc tttagcctct tccgtcttaa agtagccatg gacttgtacg
    44281 accttttagc aggtgtgagc aacacgtcag ctgcgaacaa ggttttgagc aaggatcaag
    44341 tcttggaacg ccagccaaac ttgaagaagg aaggcttggt aggaggtgga gtgtatcttg
    44401 acttccgtaa caacgatgcg cgtctcgtga ttgaaaacat caaacgtgcc aaccaagacg
    44461 gtgccctcat tgccaaccac gtgaaggcag aaggcttcct ctttgacgaa agtggcaaga
    44521 ttacaggtgt tgtagctcgt gatctcttga cagaccaagt atttgaaatc aaggcccgtc
    44581 tggttattaa tacaacaggt ccttggagtg ataaagtacg taatttgtct aataagggaa
    44641 cgcaattctc acaaatgcgc ccaactaagg gagttcactt ggtagtggat tcaagcaaaa
    44701 tcaaggtttc acagccagtt tactttgaca caggtttggg tgacggtcgt atggtctttg
    44761 ttctcccacg tgaaaacaag acttactttg gtacaactga tacagactac acaggtgatt
    44821 tggagcatcc aaaagtaact caagaagatg tagattatct acttggcatt gtcaacaacc
    44881 gctttccaga atccaacatc accattgatg atatcgaaag cagctgggca ggtcttcgtc
    44941 cattgattgc agggaacagt gcctctgact ataatggtgg aaataacggt accatcagtg
    45001 atgaaagctt tgacaacttg attgcgactg ttgaatctta tctctccaaa gaaaaaacac
    45061 gtgaagatgt tgagtctgct gtcagcaagc ttgaaagtag cacatctgag aaacatttgg
    45121 atccatctgc agtttctcgt gggtctagct tggaccgtga tgacaatggt cttttgactc
    45181 ttgctggcgg taaaatcaca gactaccgta agatggctga aggagctatg gagcgcgtgg
    45241 ttgacatcct caaagcagaa tttgaccgta gctttaaatt gatcaattct aaaacttacc
    45301 ctgtttcagg tggagaattg aacccagcaa atgtggattc agaaatcgaa gcctttgcgc
    45361 aacttggagt ttcacgtggt ttggatagca aggaagctca ttacctagca aatctttacg
    45421 gttcaaatgc accgaaggtc tttgcacttg ctcacagctt ggaacaagcg ccaggtctta
    45481 gcttggcaga caccttgtcc cttcactatg caatgcgcaa tgagttgact cttagcccag
    45541 ttgacttcct tcttcgtcgt accaatcaca tgctctttat gcgtgatagc ttggatagta
    45601 tcgttgagcc agttttggat gaaatgggac gattctatga ctggacagaa gaagaaaaag
    45661 caacttaccg tgctgatgta aaagcaactc ttgctaataa tgatttagca gaattaaaaa
    45721 attaagaaaa agtaaaagag gtggagggta gcaatccttg tcgcccgccc cttttttaat
    45781 ggagacagaa aaatgatgag tgaattattt ggagaatttt tagggacttt aatcctgatt
    45841 cttctaggaa atggtgttgt tgcaggtgtg gttcttccaa aaaccaagag caacagtgca
    45901 ggttggattg tgattactat gggttggggg attgcagttg cggttgcagt atttgtatct
    45961 ggaaaactca gcccagctca tttaaatcca gctgtgacag ttggtgtagc tttaaaaggt
    46021 gacttgcctt gggcttccgt tttgccttat atcttagccc agttcgcagg ggctatgctg
    46081 ggtcagattt tggtttggtt gcaattcaaa ccgcattatg aggtagaaga aaatgcaggc
    46141 aatatcctgg caaccttcag tactggacca gccatcaagg atactgtatc aaacttgatt
    46201 agcgaaatcc ttggaacttt tgttttggtg ttgacaatct ttgctttggg tctttacgat
    46261 tttcaggcag gtatcggaac ctttgcagtg ggaactttga ttgtcggtat cggtctatca
    46321 ctaggtggga caacaggtta tgccttgaac ccagctcgtg accttggacc tcgtatcatg
    46381 cacagcatct tgccaattcc aaacaaggga gacggagact ggtcttacgc ttggattcct
    46441 gttgtaggcc ctgttatcgg agcagccttg gccgtgcttg tattctcact tttctaatct
    46501 agaaaacaat tatgttgata gagcttgggc aagagcccaa tttcagcaaa aaatgaagta
    46561 aatcttctca taataaaacg catcatatca agcacgaaaa ttccacgagg tcaactacag
    46621 tcagaaagct gaacaacaag ccaaaacgcc caaaaaaggc ggcaaaaagc aagcacctgc
    46681 aagcaacgtg ccgaaatagt caaattctga ttatgtcaac gaattagacc caaaaatcgt
    46741 tgatatgcta gtagaatttc acaagtcaca aggcactttg gaaactcccg aggcgcaagc
    46801 agaaatcgcc caaaaacgtg aagaaatcga gcaaaggaga gctgagcttg aggataaaaa
    46861 acaagagctt ttgaaccgct tgaacaaata gagtttcgca agtattatgc ttacaaatta
    46921 cttgagcaat taactaaaat ataaaccctg cctttatatc taggcagggt ttatatttta
    46981 gaaattcacg taggttgtta cggtttttac atacccagta tagtttgagt ttctatagta
    47041 ttcagtgata aacttccatt ttctttgagc aacatggata taagtacttg ttatgtagta
    47101 tggatatggg ctttgtgaat ccaagtaaga ctgataagct tgtataccaa aatatgctcc
    47161 accaattatt gcaccccatg gaccccccaa taaagcacct atcctaccaa tcatataact
    47221 gattccagca ccagtcatga agttagcgaa tgtgttagct tgtttattcc catgtattgt
    47281 gttgacgtaa tttcaaacat taggatcgta tgatctaaaa gatatattta ggtcgatttc
    47341 attcttttga taagccatat aaaatgcccc attgatatag acgccgtcag cacgtcgttc
    47401 aatagtgtct acacttccat ctggattgac aacctcaaga acttcatcgc ttaaaatatt
    47461 tacttgcgta tctccgaacc gcactgatga gccattctca aactgagcct caccagatac
    47521 aactttagag tttgccgata agctatcatc agcaaaaaca acaagcgacg gggaaatgct
    47581 agacatacag aaaacagaca taactagcaa acacatgcat ttaaacatct tagacataac
    47641 ggaaactcct ttgtattttt gatttttttc aacttttatt atacaataaa accaaataaa
    47701 aagaaagcgg taacaatatg cttaatgcga aaatttttta tatattttta tgtttgatcg
    47761 ttatcgaaac tacaggcttg ttgttgttga aaagaggtct cgaaatgggt tatttagaca
    47821 cagaagctat tatcctcgca gttttttcat ttgcttttta caacctatgt tcattcgctt
    47881 gggtctgctc tacaataaaa aacaataaaa aataaataga cgtattttca aaaaaaatgc
    47941 atatttatct tagtaaaacg acgatttaaa tcgtcgtttt tttgtagtac gacgggcatg
    48001 tcgtatatct gaggtgcaag tcctccgtgg gcacttgcta ccggtgaacc caaaagcgat
    48061 tcccaagcct gactatcgtg aggtagcggg gagaggaaga gatagcgaaa tcgtggctct
    48121 acgaacagga acgtgatagc aaggcgtata tagcggataa ggaggcttca aactctaaag
    48181 tccaaaaagg tagtcgtaac ctatatgtgt aaatcacgag agtaattgaa ttcggactaa
    48241 ggtttgtgtg aaaaagataa atctttctag agtctaaaga ctctgcgtca gatttcctat
    48301 tttcactgta accttttaac gtcctcatat cttgtataaa cgaggaaaga tgtacgactt
    48361 atcccgtgag gtttcatgag cgctgaaagc gtagtaacaa cgaatcatga gaagtcagcc
    48421 gagcccatag tagtgaggaa acttccgtaa tggaagtgga gcgaaggggt gaatactcaa
    48481 acagtctggg gagagactgt ttgaggtctg tcgctagaaa gagaaaacga cagatcgaag
    48541 taatcctact tcacttgtgt ctgtaaaatg agtggtctga tagaactgga ctttgacacg
    48601 taatgactag atgaaggttc accaatataa gatgtccctc aggcaccaaa acaagaaagg
    48661 aatacgcaca tgtcaaaatt gctagataag atattatcac gcgaaaatat gctggaagcc
    48721 tacaatcaag taaaatccaa taaaggctca gctgggattg atggaatgac tatcgaagag
    48781 atggataatt atctcagaca aaactggcgc ttgactaagg aactgataaa acagagaaaa
    48841 tataagcctc aaccagttct tagagttgag attactaaac caaacggagg tatccgtcaa
    48901 ctaggaattc caacagttat ggatagaatg attcaacagg ccattgtcca agttattagc
    48961 cccatttgtg aaccccattt ctcagatacg agttatggct tcagaccaaa taggtcatgt
    49021 gaaaaagcca tcatgaagtt cttagagtac ttaaatgacg gctatgagtg gatagtggat
    49081 atagacctag agaaattttt cgatacggtt cctcaagata gattgatgtc cttggtacat
    49141 aacattatcg aagacggaga tacggaatcc ctgattcgta agtatcttca ttcaggtgtt
    49201 atcattaatg gtcagcgtca taaaacgcta gttggcacgc cacagggagg aaatttatct
    49261 cctctcttat ccaatgtcat gcttaatgaa ttggacaagg aattagaaaa gaggggactt
    49321 cgatttgtgc gctacgcaga tgattgtgtg attacggtcg gaagcgaggc agccgctaag
    49381 cgtgtgatgt attcagccag tcgttttatt gagaagcgac taggtttgaa agtaaatatg
    49441 accaaggcta agattaccag accaagagag ctgaaatact taggttttgg gttctggaaa
    49501 tcatcagatg gttggaaaag tcgtccccat caagatagtg ttcggagatt taagcttaaa
    49561 ttgaagaaac taacacagag gaaatggagt atagacttaa caagacgtat tgagcaactg
    49621 aatttgtcta ttcgaggatg gataaactat ttctcgctgg gaaatatgaa gcgcatagtc
    49681 gctagcatag atgagcgctt gcgtactcgc ctacgagtga ttatctggaa gcaatggaag
    49741 aagaaatcga gacgactatg gggattgctt aagttaggag ttcctaaatg gatagcagat
    49801 aaggtatctg gctggggcga ccattatcaa ttagtagctc agaagtcggt acttaaacgt
    49861 gctatatcaa aaccagttct tgaaaaacgt ggactggttt cgtgtttgga ttattacctt
    49921 gaacgacatg cgttaaaagt tagttgaacc gccgtatgcc gaacggcatg tacggtggtg
    49981 tgagaggggc tagagattat cccctactcg attaactccc ctgaaattta ttttaattat
    50041 gcaaatttca cgtatttttg atgctgagac gacgatctgg gaacttttca gatatttttt
    50101 tgactatcta aaatctatca ttagaaaagc ttagagcgcc aaaggatttg agcgtttttc
    50161 tgatttttaa gactttttcc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
    50221 taactaactt cttagtacta gccaacaacg ataatcataa ttcctcctaa aattaggaat
    50281 aataaaggca atagtttttg ttttttcatg taaaaaacct cacttttgtt ttctgctatt
    50341 ttatgctaaa atattaaaaa tcaaatttaa ttccaaagtt tgtaactaaa gggggagcgc
    50401 tacatgtcta attcatttgt caagttgtta gtctctcaat tatttgcaaa tttagcagat
    50461 attttcttta gagtaacaat cattgctaac atatacatta tttcaaaatc agtaattgcc
    50521 acatcactag ttcctatctt aataggaata tcctcttttg ttgcgagtct tttagttccg
    50581 ttggttacta aaaggttagc gctaaatagg gttttatctt tatctcaatt tggaaagact
    50641 atattattgg cgatactggt aggaatgttt accgtaatgc aatccgtagc gcctttggtg
    50701 acctatctat ttgttgttgc aatttccata ctagatggtt ttgcagcacc cgtttcctat
    50761 gctattgtgc cacgctatgc gaccgatttg ggtaaggcta attcagcctt atcaatgact
    50821 ggtgaagctg ttcaattgat aggttgggga ttaggtggac tcttatttgc aacaattggt
    50881 ctgttaccta ccacgtttat cattttagtc ttgtatatca tttctagctt tctgatgtta
    50941 tttcttccta acgctgaagt ggaggtgtta gagtcagaaa ctaatcttga aattttgctc
    51001 aaaggttgga agttagttgc tagaaatcct agattaagac tttttgtatc agcaaattta
    51061 ttggaaattt tttcaaatac gatttgggtt tcttccatta tacttgtttt tgtaacggag
    51121 ttattaaata aaacggaaag ttactgggga tattctaata cagcatactc tattggtatt
    51181 ataattagtg gcttaattgc ttttaggcta tctgaaaagt tccttgctgc taaatgggaa
    51241 agtattcttt ttcctcttgt agctatggca atagtgacac tgactattct atattttcca
    51301 aacgcacaga tgtttttatt attttcagct ttagttggta tgttatcgca actaaaagaa
    51361 gttcctgaaa gtgtatttct tcaggaaaca gtagaggaaa atcatttagt taatgtctat
    51421 tccgttcttg aagtgatttc tacattagca ttttcagtat ttgttttgct aatgagctat
    51481 attactgaga gttttggtat tagcatcagt ttttggctat cagccatttg tctgatgata
    51541 gaagctattt taatttatat cagacgagat tattttaaat gagagagtgg ttaaaaagtc
    51601 tttaaaaatc agaaaagcgc atagtatcag gtgttgaaaa aacttgatat tgtgtgtttt
    51661 attatgggaa gatttacttc attgtctcct gaaatggagt tttgaagcag gatcactcta
    51721 tttgaaaata tacatagaaa aactgtataa tccttaaaat aagctgaaat ctattggaaa
    51781 catacggtaa acaattgaaa agatactata taaagtggta caatggtaga aaaataagct
    51841 agtaagaatc actcttattt tagcgaaaaa ttacagaaat gaatggtttt tcttgatgaa
    51901 acagagaaaa gaattgtacc tctttcttgg tcggacagcc ttgtattttc ttatctttct
    51961 agggctgctt tacttcttta gctatcttgg tcagggtcaa ggaagcttta tctataatga
    52021 attttaactt gaaggagaat ccctattgtg tcaaataaac caatagcaga tatgattgaa
    52081 accattgagc attttgctca gacacagcct agctatcctg tctataatgt tttggggcag
    52141 gaacacactt atggcgattt aaaggctgat tcggatagtt tggctgcagt cattgaccaa
    52201 ctaggcttgc ctgagaagtc tcctgtggtt gtttttggtg gccaagaata tgaaatgttg
    52261 gcaacctttg tagcgctgac taagtcaggt catgcctaca ttccaattga tagccattcg
    52321 gccttggagc gagtttcagc tattttagaa gtagcagagc caagcttgat tattgccatt
    52381 tcagcctttc ccttggagca ggtttctaca ccaatgataa atctagctca ggttcaagaa
    52441 gcctttgccc aagggaacaa ctatgaaatc acgcatccag tcaagggaga tgataattac
    52501 tacattatct ttacttctgg tacgactggt aagcctaagg gagtgcagat ttcacatgat
    52561 aatctcctca gctttacaaa ctggatgatt acggataagg aatttgcgac accgagtcgt
    52621 ccgcaaatgc tggcacagcc accttattct tttgacttgt ctgtcatgta ttgggcaccg
    52681 accttggcac ttggtggtac gcttttcact cttccttcag tcatcactca ggactttaag
    52741 caactctttg cggctatctt ttcattgcca atcgctatct ggacatcaac accatccttt
    52801 gcagatatgg ccatgttgtc tgaatacttc aacagtgaga aaatgtctgg aatcacgcat
    52861 ttctactttg atggtgaaga attgacggtc aaaacagctc aaaaactgcg cgagcgtttc
    52921 ccaaatgccc gtatcatcaa tgcttacggc ccaacagaag cgacagtagc tctgtcagca
    52981 gttgccgtga cagacgagat gttagcgact ctcaaacgcc taccaatcgg ctataccaag
    53041 gctgattctc caacctttat cattgacgag gaaggaaata aactgccaaa tggtgagcag
    53101 ggagaaatca ttgtttctgg gccagctgtt tcaaaaggtt atatgaacaa tcctgaaaaa
    53161 acagcagaag ccttctttga gtttgaagat ctgccagcct atcacacagg cgatgtggga
    53221 accatgacag atgagggctt gcttctctac ggcggacgca tggacttcca gattaagttt
    53281 aacggttacc gcattgagtt agaagatgtc tctcaaaacc tcaacaagtc tcgctttatc
    53341 gaatctgctg tcgcagtacc gcgctataac aaggaccaca aggtacaaaa tctattggct
    53401 tatgtcatct taaaagacgg tgttcgtgag cagtttgagc gagatatcga tattaccaag
    53461 gccatcaagg aagacctgac agacatcatg atgtcctata tgatgccatc taaattcctt
    53521 taccgagaca gtttgccact gactccaaat ggaaagattg acatcaaagg attgattaac
    53581 gaggtgaata agagatgatg gagttttttc aacagcttcc tcatttagag ccatatggca
    53641 atcctcagta ttttgtttat gtgattgctg caaccttgcc catcttcata ggcctctttt
    53701 tcaagaaacg ctttgcctgg tatgaagtgt tggtaagtct cttctttatt gtcaccatgt
    53761 tggtgggtgg aaagaccaat caactagctg ccttgggtat ttacctttgc tgggaaatat
    53821 tgctcctgct tttctacaag cattatcgaa aaagcaagga tggcaagtgg gtcttctact
    53881 tagttagttt tctgtcccta cttccgatta tctttgtcaa ggtgcaacca gctatcaatg
    53941 gaacgcagtc tttgcttggg ttcttgggaa tttcttatct gacctttcgt tcggttggga
    54001 ttatcatcga gctgagagat ggagtgatta aggattttac cctctgggaa ttcctccgtt
    54061 tccttctctt catgccaact ttctcgagtg gtccaatcga tcgttttaag cgatttaatg
    54121 aaaattatca ggccattcct gagcgagatg agttgatgga tatgctggat gaatctgtcc
    54181 gctatatcat gcggggcttt ttgtataagt ttatcctagc tcatgtttta ggagagacct
    54241 tactacctcc tctgaagaat ttagccttgc agtcaggtgg cttctttaat ctctatgcct
    54301 tggcagttat gtatactttt ggtctggagc tcttctttga ctttgcaggt tattctatgt
    54361 ttgctttggc catctcaaac ttgatgggaa tccgtagccc tatcaacttt aacaagccct
    54421 ttttatcaag ggatttaaag gagttttgga atcgctggca tatgagtctg tccttctggt
    54481 tccgtgactt tgtctttatg cgaatggtga tggtgttaac cagaaagaaa gtctttaaaa
    54541 atcgtaatgt aacctcaagc atggcctaca ttgtaaatat gctgattatg ggattttggc
    54601 atggcgtgac ctggtactat atcgcctatg gactctttca tggactaggc ttggtcatca
    54661 atgatgcctg ggttcgcaag aaaaaaacgc tcaataagga acggaaaaaa gcagggaagg
    54721 ctgccctacc tgagaatcgc tggattcagt tgcttggcat ggttgtcact ttccatgttg
    54781 tcatgttgtc attcttaatc ttttctggat tcttgaataa tctatggttt aaaaaataaa
    54841 aggaaataaa atatggatat caaatcagaa gttattgaaa ttattgatga gttgtttatg
    54901 gaagatgttt ctgacatgat ggatgaagat ctttttgatg caggtgtctt ggatagtatg
    54961 ggaacggttg agttgattgt ggagattgag aaccgttttg acattcgtgt ccctgtaaca
    55021 gagtttggtc gtgacgactg gaatacagct aataaaatca tagctggtat tgtggagcta
    55081 caaaatgctt aaacgcttat ggatgatctt cggaccggtc ttgatcgctg gtttgttggt
    55141 ttttctgctc attttctttt atcctactga gatgcatcat aatctaggag ctgaaaagcg
    55201 ttcagcagtg gctactacta tcgatagttt taaggagcga agtcaaaaag tcagagcact
    55261 atctgatcca aatgtgcgtt ttgttccctt ctttggctct agtgaatggc ttcgttttga
    55321 cggtgctcat cctgcggtat tagctgagaa atacaatcgt tcctaccgtc cttatctttt
    55381 aggacagggg ggagctgcat cgcttaacca atattttgga atgcaacaga tgttaccaca
    55441 gctggagaat aaacaagttg tgtatgttat ctcacctcag tggttcagta aaaatggcta
    55501 tgatccagca gccttccagc agtattttaa tggagaccag ttgactagtt ttctgaaaca
    55561 tcaatctggg gatcaggcta gtcaatatgc agcgactcgc ttactgcaac agttcccaaa
    55621 cgtagctatg aaggacctga ttcagaagtt ggcaagtaaa ggagaattgt cgacagcaga
    55681 caatgaaatg attgaattat tggctcgttt taatgaacgc caagcctcct tttttggtca
    55741 gttttcggtt agaggctatg ttaactacga taagcatgta gctaagtatt taaaaatctt
    55801 gccagaccag ttttcttatc aagcaataga agatgttgtc aaagcagatg ctgaaaaaaa
    55861 tacttccaat aatgagatgg gaatggaaaa ttatttctat aatgagcaga tcaagaagga
    55921 tttgaagaaa ttaaaggatt ctcagaaaag ctttacctat ctcaagtcgc cagagtataa
    55981 tgacttgcag ttggttttaa cacagttttc taaatctaag gtaaacccga tttttatcat
    56041 tccacctgtt aataaaaaat ggatggacta tgctggtcta cgagaggata tgtaccaaca
    56101 aacggtgcag aagattcgct accagttaga aagtcaaggt tttaccaata tagcagattt
    56161 ttctaaggac ggcggggagc ctttctttat gaaggacacc attcaccttg gttggttggg
    56221 ttggttggct tttgacaagg cagttgatcc tttcctatcc aatcccacac cagctccgac
    56281 ttaccatctg aatgagcgct ttttcagcaa agattgggcg acttatgatg gagatgtcaa
    56341 agaatttcaa tagataatat aatatagaag agttcaagaa tgaaactagt tttcacgttt
    56401 tttcttgact ctttttttgc ttgtgatata attaactgat aaacaaaatg tcaaagaata
    56461 gtatttttct cttaaaaaag agaagtctaa aaggaaagga gtcagatatg agacagctag
    56521 caaaggatat caatgctttt ttgaatgagg tgattttgca ggcggaaaat cagcatgaaa
    56581 tcctaatagg tcattgcact agcgaggtgg ccctgaccaa tactcaggag catatcctta
    56641 tgctcttgtc agaggaatct ttaacaaatt cagaattggc ctgtcgtctc aatgtcagtc
    56701 aggcggcagt taccaaggcc attaagtctt tggtcaagga agggatgttg gaaacatcta
    56761 aagattctaa agatgcgcgt gtgatttttt atcagttgac tgacttggct cgtccaatcg
    56821 ctgaggagca ccaccatcac catgagcata cacttttaac ctatgaacaa gtggcgactc
    56881 agtttactcc aaatgaacaa aaagtgattc agcggttttt gactgcttta gtaggagaaa
    56941 tcaaataatg agatatatta cggtagagga tttgtccttc tattatgata aggagcctgt
    57001 tcttgaacat atcaattatt gtgttgatag tggggaattt gttaccttga ctggggaaaa
    57061 tggagcggct aagacgacgc tcatcaaggc tagtcttgga attctgcaac cacgcattgg
    57121 aaaggtggct atttcaaaga caaatacgca aggtaagaaa ttgagaatag cctatcttcc
    57181 tcaacaaatt gccagtttta atgctggttt tccaagtacg gtctatgaat ttgtcaagtc
    57241 gggtcgctat ccgagaaaag gctggttccg tcgtttgaat gctcatgatg aggagcatat
    57301 caaggccagt ctggactcag ttggcatgtg ggaacatcga gacaaacgct tggggtctct
    57361 atctggggga caaaagcagc gagcggtaat tgcgcgtatg tttgcttctg accctgatgt
    57421 gtttatccta gacgagccga caacggggat ggatgcagga agtaaaaatg aattttacga
    57481 actcatgcac cacagcgccc atcatcatgg caaggctgtt ttgatgatta cccatgaccc
    57541 tgaagaagtt aaggattatg cggatcgcaa tattcatcta gtccgtaacc aagactcgcc
    57601 atggcgttgt ttcaatgttc atgagaatgg ccaggaggtg ggccatgctt agtttattat
    57661 cttacgactt tatacagcgc gcctttttgg cggttattgc tatgagtctt ttctcaccgg
    57721 tattgggaac cttcctcatc ttgcgtcgtc agagtttgat gagtgatacc cttagccacg
    57781 tctcactttc aggtgtagcc tttggtctgg ttttggggat ttctccaact gtttctacta
    57841 ttgccattgt cttgattgcg gcggtctttc tggagtatct ccgtacggtt tacaagagct
    57901 ttatggaaat cgggacagct atcctcatgt caacaggtct ggctgtttct ctgattgtca
    57961 tgagcaaggg taaaagctcg agttcaatga gtttggacca atatctcttt ggttcgatcg
    58021 tgactatcag tgaagaacag gtcatttccc tctttgtcat tgcggcggtt gttttgattt
    58081 tgacctttct ctttcttcgt cctatgtata tcttaacttt tgacgaagat acggcctttg
    58141 tggatggctt gccagttcgt accatgtcca ttctttttaa catggtgaca ggggtggcta
    58201 ttgcccttat gattcctgca gcaggagctc ttctggtatc gaccattatg gtcttgccag
    58261 ctagtattgc cctgcgtctg gggaaaaact ttaaatcggt tatgctgctt gccagtgcga
    58321 ttggcttttt gggaatggta gcaggacttt acatttccta ctatgcagaa acacctgcaa
    58381 gtgcaagtat taccattatt tttgtaactg tctttatact aatcagttta gtaagacgtt
    58441 ttatcaaata ggagacgaac gtgaaaaaaa ttagcttatt actagccagt ctatgtgcct
    58501 tgtttttagt ggcttgttcc aatcaaaaac aggcagatgg taaactcaat atcgtgacaa
    58561 ccttttaccc tgtctatgaa tttaccaagc aagtcgcagg agatacggct aatgtagaac
    58621 tcctaatcgg tgctgggaca gaacctcatg aatacgaacc atctgccaag gcagttgcca
    58681 aaatccaaga tgcagatacc ttcgtttatg aaaatgaaaa catggaaaca tgggtaccta
    58741 aattgctaga taccttggat aagaaaaaag tgaaaaccat caaggcgaca ggcgatatgt
    58801 tgctcttgcc aggtggcgag gaagaagagg gagaccatga ccatggagaa gaaggtcatc
    58861 accatgagtt tgacccccat gtttggttat caccagttcg tgccattaaa ctagtagagc
    58921 acatccgcga cagcttgtca gcagattatc ctgataaaaa agagaccttt gagaagaatg
    58981 cagctgccta tatcgaaaaa ttgcaagcct tggataaggc ttacgcagaa ggcttgtctc
    59041 aagcaaaaca aaagagcttt gtgactcaac acgcagcctt taactatctt gccttggact
    59101 atggactcaa acaagtcgca atctcaggac tttctccaga tgcagagcca tcagctgctc
    59161 gcttggcaga attgacagag tatgtcaaga aaaataaaat cgcctatatc tattttgaag
    59221 aaaatgcctc acaagccctt gctaacacac tttcaaaaga agcaggtgtc aaaactgatg
    59281 tcctcaatcc tttagaaagt ctgacagaag aggacaccaa ggctggagaa aactacattt
    59341 ccgtgatgga gaaaaacctc aaggctttga aacaaacaac agaccaagaa ggtccagcaa
    59401 tcgaacctga aaaggcagag gataccaaga cagtccaaaa tggttacttc gaggatgcag
    59461 ctgtcaagga ccgcaccttg agtgactatg caggtaactg gcaatcagtt tatcctttcc
    59521 ttgaagacgg cacgtttgac caagtctttg actacaaggc taagttgact ggtaagatga
    59581 cccaggctga gtacaaggct tactatacaa aaggctatca gacagatgtg actaagatta
    59641 acattactga taatactatg gaatttgttc aaggtggaca aagcaagaaa tacacttaca
    59701 agtatgtcgg taagaaaatt ttgacttaca agaaaggcaa tcgtggcgtg cgtttcctct
    59761 ttgaagccac agatgctgac gctggacaat tcaagtatgt tcagtttagt gaccacaata
    59821 tcgccccagt taaggcagaa catttccata tcttctttgg aggcacaagc caagaagccc
    59881 tctttgaaga aatggacaac tggccaacct actacccaga taacctatct ggccaagaaa
    59941 tcgctcaaga aatgttggcg cattgatgag aaatcgacta gttcataaga gctagtcggt
    60001 tttttgatac tagatggcta acttcattat agacctttca gaactaaggg aacctgaaaa
    60061 aataaataaa actcttgaaa tatctgggct tatatgctag aatgtagtta gtgtataagg
    60121 ttcattagaa caccaaagcc cctaactagt gccatagtta agggcttttt tgacgtatct
    60181 tagcgattta acgggtgttt ataggctagt cagtcggtct acttcttacc atctagccat
    60241 ttgcagacga aatacaagat aattccagct ataacatctg ctataatagt aagagcaagc
    60301 tctggtataa ggctcattag tttcacctcc tctcacgaac cgtaaggaac gtaatcggta
    60361 accgatgaca aaaatagtat atcacattaa atttagatca tcaacattgc ttaatgcttg
    60421 aaatattagg acttatatgc taaaatgtag ttaattgata aggttcatta gaacaccaaa
    60481 gcccctaact atggccgtag ttaagggctt ttttgacgta tcttagcgat ttaacgggtg
    60541 tttataggct agtcagtcgg tctactcctt actatctagc catctgcaga cgaaatacaa
    60601 gataattcca gctataacat ccgctataat agtaagagcg agctctatga taaggctcat
    60661 tagtttcacc tcctctcacg aacccatagg aacgtaatcg gtaaccgatg acaaaaatag
    60721 tataccacaa tacatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc
    60781 gacaaagtct ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat
    60841 gcgttttatt gtgggaatat ttgccccatt tctcctgaaa ttgagttttt gtccagcctc
    60901 tgtttttagg gttgctaaga aaataatgtc atgtggtgaa tatttgtaaa tcagtcagca
    60961 gacagaacga tactcttcga aaatctcttc acatcatgtc agcttcgtct ttccgtatat
    61021 atgtgactga cttcatcagt tctatctaca acctcaaaac agtgtttcga gctgacttga
    61081 tcaattttca aatctgtact ttgagcaagc tgagactagc ttcctatttg attttcattg
    61141 aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt tttggtctgg
    61201 cacggtgtca gaaataaagg ttgtgtattt ggagagggga ttaattttaa aaaatccagt
    61261 cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt caataatatt
    61321 cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat caatacctct
    61381 tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt ccttatcata
    61441 gttgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga ttttggagct
    61501 atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa aaataatcaa
    61561 attatcaagt tcagaaagat agggacaaag ttcgtagaca gtagtactag aatctagata
    61621 gatacacata ccagaccgaa taaagtcttt agcgagacta gcgattagtc ttttttgcct
    61681 agtactttct ccttcacgta tttgatgaga aagttcaatt gtgttcatag aggacagggt
    61741 cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta aatcacgacg
    61801 taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa ttcttttttt
    61861 ctttttgata atttcaatca attcaagtac acgttcatct tttatcataa gctcctccta
    61921 atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat ttttatgaat
    61981 attgggttaa catttgaaca ttattcaagt aagcgttcac atattgaaaa aataaaacgt
    62041 ggggattata ataaagttaa tcaaggacga agagagaaga aaaatggaag cggttttagc
    62101 aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg aaaataaact
    62161 agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag ggcatttatc
    62221 ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg ctaatactag
    62281 ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac tgattgataa
    62341 tgaaggtaag ctgttattac aacctgttca ttatcgtgat gaaagaacaa agggagtgtt
    62401 aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag gaaatcagat
    62461 tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc ctgactcttt
    62521 ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct tgacaggtaa
    62581 gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta ggagtcaaaa
    62641 ttggaatcag aatatcttaa aactatttga attggattca tctttacttc ctgaaattgt
    62701 ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg atattcctgt
    62761 tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta agacagaagg
    62821 tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta cttcaccgat
    62881 tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag atggagagat
    62941 tacatttctg aagaattgta cagggttgtg gattatagag gaactaagac gttcatttga
    63001 acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga aagaaaaaga
    63061 aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg atatgcacaa
    63121 gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag atggacaact
    63181 atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag agttactaga
    63241 agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg ctagagccag
    63301 ttattttaac caaatgattg ctgatagaac tggtaaagag gttcttacag gttcgactga
    63361 gggtacagct gtggggaata ttgttgtgca gctcatagct atgggacaat taaaagggat
    63421 ggaagaagct caccatgtta ttgaggagtt cctacaatta gagagttatt actcccaaaa
    63481 gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttag cttttgtgca
    63541 ggaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga ggaggacaag
    63601 gatgtcagat gtaaaacaag aattaattaa atatggtaag aagctagtag aaacagattt
    63661 gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat tgatggcaat
    63721 taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag tgatggatat
    63781 taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata tgcatttgat
    63841 tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa cttatgcaac
    63901 agtattagct tgtctcagag aaccacttcc agcgagtcat tatatgattg cagtggcagg
    63961 gaaagatgtt cgggtagctg agtatgcaac atatggcacg aaagaattgg ctgtgaatgc
    64021 agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa ttttagcagg
    64081 tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt gtgcaaaaat
    64141 ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg agatggaatt
    64201 gatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt aatgttaaaa
    64261 catataccga aaaatatttc tccagattta ttgaagactt taatggaaat gggacatgga
    64321 gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa taagctaatt
    64381 cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt aatgccatta
    64441 gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga tgatattcct
    64501 aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct taaaacgatt
    64561 acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc tattgttgct
    64621 acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt tgttgaaaaa
    64681 gaaaatgttt aatagaggaa ttttagttgc cagtcatggt gattttgcta gcggagctct
    64741 catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga cattaggttt
    64801 gatgcctgga gagaatattg tagagtttga gcattatttt aaaaatcaag tggatgaact
    64861 gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa gtcctaataa
    64921 tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag ggtttaatat
    64981 tcctctccta gtggaattaa tatcaaatta tgattcaaaa atcaatttag aagaaattgt
    65041 tcacaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag aggaggaaga
    65101 agatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg tcaagttgtc
    65161 actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga tcgcatctca
    65221 gaagataaaa cacgacaatc tattttaaag atttctgcac cagtaggttt aaaaattgtt
    65281 ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa aaagagaaca
    65341 atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa tttaaaattg
    65401 gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt aacgggaggt
    65461 gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa gggaacgaga
    65521 gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt tttataaaaa
    65581 taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac attagttggg
    65641 attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc gttagttaca
    65701 agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat tggttcagct
    65761 cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc agatactatt
    65821 tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga aactgctggt
    65881 atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct tgcaaaaact
    65941 ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga ttattcaaag
    66001 atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat tgtaccaatt
    66061 ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa ggttccacca
    66121 atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg ttttggtatg
    66181 cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg attcatttgt
    66241 tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc ggtagcatac
    66301 ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga tgttgaggag
    66361 gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa gttttcaaac
    66421 gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta ggttttctat
    66481 atacaattct tccagtattg aaaaaactat acccagacaa agattcagct tctcctgcaa
    66541 tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt attcttggag
    66601 ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt actggtatta
    66661 aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg ctgacactag
    66721 ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct ttaggtatct
    66781 ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc ggtttgaaat
    66841 atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga acattgaatc
    66901 gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg attccatcaa
    66961 tggttggtat taattttgga ttagaattta agcaggggga acttgttatt tctgttcaag
    67021 aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta ttaatgtgta
    67081 aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg gctattggag
    67141 ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg tatttgggat
    67201 atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa agctgcctca
    67261 aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat ttatggttca
    67321 gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg aaagtcagat
    67381 agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct tttagatgga
    67441 gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc aagagatcaa
    67501 agccactatg aattacttgg ggaactttac attgagcata tagatattca gtcttgtgct
    67561 ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt tgtgtttgag
    67621 cctaatagtt gtaatttaca aataaaaaga gaatatttta cgaattttaa taagaatatt
    67681 ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat taatttgggt
    67741 agaaataaac ggtttaatga tgaagtatct aaactggatt caagtacaat tttaatgtcg
    67801 gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc taaggttacg
    67861 gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac agaggtattt
    67921 ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc tcttcaggga
    67981 gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa atatcaggag
    68041 caatttaata gagttgattt taaactagac tatagtaaag attgtcttag cattccaacg
    68101 aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt gttatttcat
    68161 tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgc caatcttcaa
    68221 ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat taatattaat
    68281 actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga atatccatta
    68341 tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa aatgtatgga
    68401 gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc tccccaatct
    68461 catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac tcatatttgg
    68521 gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga aatgataaaa
    68581 gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt gatgacaggt
    68641 ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg aaatgcttgt
    68701 ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg cattggaatt
    68761 gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt aaaaaagaaa
    68821 ttacctagaa caaaaatagg tagtaatggg caaatacaag aatggctaga ggattatgaa
    68881 gaagtagagc ctggtcatag acacatttca cctctatttg ggctttatcc ttataatgag
    68941 attgatattc ataaaactcc ggaattagca gaagcagcta aaatcacgat caataggaga
    69001 ttatcaaacg ctaacttttt atcttcacag gagagggagc aagcgattaa taattggtta
    69061 gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct gattcatttt
    69121 tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt gttaaataat
    69181 gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg taatttaggt
    69241 ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt atcactaatt
    69301 ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt aagaggagga
    69361 tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt ggaaggagga
    69421 aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt acaaaatatt
    69481 gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata agtcatgaat
    69541 aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg gatgttatgt
    69601 tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac gacgattaat
    69661 aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa cggattatgg
    69721 tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa accatatgca
    69781 gcgattgaac ttcatcctgc aaaagtctgt aaaccaacaa gttgtattcc acgagatacg
    69841 aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct aaacattcca
    69901 gttttcttgg ttattatgtc ggctggagag cgtaatacag ttcctccaga gtggttagat
    69961 gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa ttattggatt
    70021 tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc caaatatgga
    70081 gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat gaatgatccg
    70141 acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac taaaaatacg
    70201 ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt gagtggctta
    70261 tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca ttatacaaac
    70321 acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc agaatcaatg
    70381 attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa tttcgaatgt
    70441 gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa aggtattatt
    70501 cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt tgtaaataga
    70561 acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg attttatcaa
    70621 ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata tcatattctt
    70681 cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc taatgcaaaa
    70741 attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa ctcgctttat
    70801 ccaaaacttt atgaaggaga tggatatgct cagcgtgtag gtaattcctg gtatatttat
    70861 aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta tactaataat
    70921 acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa agaaaatcca
    70981 aataatttac atattttatt gaataattac aggacagata agacagctat gtgggcatta
    71041 tcaggaaatt ttgatgcatc aaaaagttgg aagaaagaag aattagagtt agcgaactgg
    71101 ataagcaaaa attattccat caatcctgta gataatgact ttaggacaac aacacttaca
    71161 ttaaaagggc atactggtca taaacctcag ataaatataa gtggcgataa aaatcattat
    71221 acttatacag aaaattggga tgagaatacc catgtttata ccattacggt taatcataat
    71281 ggaatggtag agatgtctat aaatactgag gggacaggtc cagtctcttt cccaacacca
    71341 gataaattta atgatggtaa tttgaatata gcatatgcaa aaccaacaac acaaagttct
    71401 gtagattaca atggagaccc taatagagct gtggatggta acagaaatgg taattttaac
    71461 tctggttcgg taacacacta gggcagataa tccctcttgg tgggaagtcg atttgaaaaa
    71521 aatggataaa gttgggcttg ttaaaattta taatcgcaca gatgctgaga ctcaacgtct
    71581 atctaatttt gatgtgattc tatatgacaa taatagaaac gaagttgcta agaaacatgt
    71641 taataatttg tcgggtgaat ctgttagtct agatttcaaa gaaaaaggag caaggtatat
    71701 taaagttaaa ttactaacga gtggagtgcc tttgagttta gcagaagtag aggtttttag
    71761 agaatcagat ggtaagcaat ctgaagagga tatagataaa ataacagaag ataaagtagt
    71821 ctctacaaat aaggtagcta ctcaaagttc aaccaattat gagggtgtag ctgctttagc
    71881 agttgatggt aataaagatg gagattacgg acatcattcg gtgactcata ctaaggcaga
    71941 ttctaacgct tggtggcagg tcgatctggg agaagagttt acggtttcta aagttgatat
    72001 ttataataga acagatgccg aacctcagcg tttatctaat tttgatgtta tttttctatc
    72061 ttcatcagga gaagaagttt ttagaagaca ttttgataaa gtagttgatg gtttgttatc
    72121 tttaaaagta ccttctgtag gggctaagct agtcaaaata gaattaaaat cagcagctat
    72181 tccgttaagt ttagcggaag ttgaagtcta tggttcaaag agaactccga agaaactttc
    72241 taatattgca ttaaccaaag aaactcgaca gagttcaacg gattacaatg gtttttctcg
    72301 tctagcagtt gatggaaata aaaacggaga ttatggtcat cattcagtga ctcataccaa
    72361 aggagattct ccttcatggt gggagataga tttagcacaa accgaagaat tagaaaagtt
    72421 aattatttat aatagaacag atactgaaat tcagagatta tcaaattttg atattattat
    72481 atatgattca aataatcatg aagtttttaa acaacatatt gacagtttag aaagcaa
//
LOCUS       DAVGJJ010000002        53832 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000002 DAVGJJ010000000
VERSION     DAVGJJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.002"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..74
                     /locus_tag="VVE35_000070"
     tRNA            2..74
                     /locus_tag="VVE35_000070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VVE35_000071"
     tRNA            99..171
                     /locus_tag="VVE35_000071"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VVE35_000072"
     tRNA            177..258
                     /locus_tag="VVE35_000072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VVE35_000073"
     tRNA            277..349
                     /locus_tag="VVE35_000073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VVE35_000074"
     tRNA            372..443
                     /locus_tag="VVE35_000074"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VVE35_000075"
     tRNA            451..536
                     /locus_tag="VVE35_000075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VVE35_000076"
     tRNA            546..619
                     /locus_tag="VVE35_000076"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VVE35_000077"
     tRNA            635..708
                     /locus_tag="VVE35_000077"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VVE35_000078"
     tRNA            714..787
                     /locus_tag="VVE35_000078"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VVE35_000079"
     tRNA            801..874
                     /locus_tag="VVE35_000079"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VVE35_000080"
     tRNA            885..974
                     /locus_tag="VVE35_000080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VVE35_000081"
     tRNA            985..1058
                     /locus_tag="VVE35_000081"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VVE35_000082"
     tRNA            1062..1134
                     /locus_tag="VVE35_000082"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VVE35_000083"
     tRNA            1154..1224
                     /locus_tag="VVE35_000083"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VVE35_000084"
     tRNA            1260..1333
                     /locus_tag="VVE35_000084"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VVE35_000085"
     tRNA            1340..1427
                     /locus_tag="VVE35_000085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1553..2488)
                     /locus_tag="VVE35_000086"
     CDS             complement(1553..2488)
                     /locus_tag="VVE35_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU3060354.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2485..3345)
                     /locus_tag="VVE35_000087"
     CDS             complement(2485..3345)
                     /locus_tag="VVE35_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3060355.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3453..5615
                     /locus_tag="VVE35_000088"
     CDS             3453..5615
                     /locus_tag="VVE35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU3060356.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRTTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYKNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGITVILSQGDFLSRQYIFRRL"
     gene            5650..6909
                     /locus_tag="VVE35_000089"
     CDS             5650..6909
                     /locus_tag="VVE35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3060357.1"
                     /translation="MEWYKKIGLLATTALALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            6923..7789
                     /locus_tag="VVE35_000090"
     CDS             6923..7789
                     /locus_tag="VVE35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3060358.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7803..8636
                     /locus_tag="VVE35_000091"
     CDS             7803..8636
                     /locus_tag="VVE35_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3060359.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            8643..8783
                     /locus_tag="VVE35_000092"
     CDS             8643..8783
                     /locus_tag="VVE35_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060360.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKATLTWFEEI
                     FE"
     gene            8994..10436
                     /gene="gtfA"
                     /locus_tag="VVE35_000093"
     CDS             8994..10436
                     /gene="gtfA"
                     /locus_tag="VVE35_000093"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU3060361.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            10574..11767
                     /locus_tag="VVE35_000094"
     CDS             10574..11767
                     /locus_tag="VVE35_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU3060362.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12453..14432
                     /locus_tag="VVE35_000095"
     CDS             12453..14432
                     /locus_tag="VVE35_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3060363.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVGDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGTAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14499..15995
                     /locus_tag="VVE35_000096"
     CDS             14499..15995
                     /locus_tag="VVE35_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060364.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15995..16921
                     /locus_tag="VVE35_000097"
     CDS             15995..16921
                     /locus_tag="VVE35_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060365.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVTIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16930..17997
                     /locus_tag="VVE35_000098"
     CDS             16930..17997
                     /locus_tag="VVE35_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060366.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18008..18934
                     /locus_tag="VVE35_000099"
     CDS             18008..18934
                     /locus_tag="VVE35_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060367.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(19009..20335)
                     /locus_tag="VVE35_000100"
                     /pseudo
     CDS             complement(19009..20335)
                     /locus_tag="VVE35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20490..21200)
                     /gene="treR"
                     /locus_tag="VVE35_000101"
     CDS             complement(20490..21200)
                     /gene="treR"
                     /locus_tag="VVE35_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU3060368.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21385..23352
                     /gene="treP"
                     /locus_tag="VVE35_000102"
     CDS             21385..23352
                     /gene="treP"
                     /locus_tag="VVE35_000102"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU3060369.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETLVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23530..25155
                     /gene="treC"
                     /locus_tag="VVE35_000103"
     CDS             23530..25155
                     /gene="treC"
                     /locus_tag="VVE35_000103"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU3060370.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRNKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25411..25659
                     /locus_tag="VVE35_000104"
     CDS             25411..25659
                     /locus_tag="VVE35_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU3060371.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            25866..26660
                     /gene="racE"
                     /locus_tag="VVE35_000105"
     CDS             25866..26660
                     /gene="racE"
                     /locus_tag="VVE35_000105"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU3060372.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKFSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26657..27628
                     /locus_tag="VVE35_000106"
     CDS             26657..27628
                     /locus_tag="VVE35_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU3060373.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27604..28125
                     /locus_tag="VVE35_000107"
     CDS             27604..28125
                     /locus_tag="VVE35_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU3060374.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28122..28583
                     /locus_tag="VVE35_000108"
     CDS             28122..28583
                     /locus_tag="VVE35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3060375.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28574..29314
                     /gene="xerD"
                     /locus_tag="VVE35_000109"
     CDS             28574..29314
                     /gene="xerD"
                     /locus_tag="VVE35_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU3060376.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFLQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRINKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29314..30042
                     /locus_tag="VVE35_000110"
     CDS             29314..30042
                     /locus_tag="VVE35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU3060377.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKVPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30061..30630
                     /locus_tag="VVE35_000111"
     CDS             30061..30630
                     /locus_tag="VVE35_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU3060378.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30617..31339
                     /locus_tag="VVE35_000112"
     CDS             30617..31339
                     /locus_tag="VVE35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU3060379.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31339..31581
                     /gene="yidD"
                     /locus_tag="VVE35_000113"
     CDS             31339..31581
                     /gene="yidD"
                     /locus_tag="VVE35_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU3060380.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(31806..32771)
                     /locus_tag="VVE35_000114"
     CDS             complement(31806..32771)
                     /locus_tag="VVE35_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3060381.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPKKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(32867..33619)
                     /locus_tag="VVE35_000115"
     CDS             complement(32867..33619)
                     /locus_tag="VVE35_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3060382.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELARELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33616..34572)
                     /locus_tag="VVE35_000116"
     CDS             complement(33616..34572)
                     /locus_tag="VVE35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU3060383.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(34562..35521)
                     /locus_tag="VVE35_000117"
     CDS             complement(34562..35521)
                     /locus_tag="VVE35_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060384.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKCYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(35925..36644)
                     /gene="galE"
                     /locus_tag="VVE35_000118"
     CDS             complement(35925..36644)
                     /gene="galE"
                     /locus_tag="VVE35_000118"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU3060385.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGRNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTH
                     DGSCIRDYIYVLDLHRLMLKE"
     gene            37045..38109
                     /gene="pepA"
                     /locus_tag="VVE35_000119"
     CDS             37045..38109
                     /gene="pepA"
                     /locus_tag="VVE35_000119"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU3060386.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38131..38427
                     /locus_tag="VVE35_000120"
     CDS             38131..38427
                     /locus_tag="VVE35_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU3060387.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            39109..39552
                     /locus_tag="VVE35_000121"
     CDS             39109..39552
                     /locus_tag="VVE35_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3060388.1"
                     /translation="MKYCHLVAHHIRLLNGRIFQKLLRQDPDSLYRSEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKNLEEQNLVTVSPCGEDKRKKYLVLTELGKSQ
                     KEVGQRVSQRLDTICYKGFSEEEIRQFEGFQERILANLKEKENEV"
     gene            39556..40320
                     /locus_tag="VVE35_000122"
     CDS             39556..40320
                     /locus_tag="VVE35_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU3060389.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVSEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40335..41063
                     /locus_tag="VVE35_000123"
     CDS             40335..41063
                     /locus_tag="VVE35_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060390.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSVHD"
     gene            41056..42576
                     /locus_tag="VVE35_000124"
     CDS             41056..42576
                     /locus_tag="VVE35_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU3060391.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            42921..43727
                     /locus_tag="VVE35_000125"
     CDS             42921..43727
                     /locus_tag="VVE35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU3060392.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(43893..44429)
                     /locus_tag="VVE35_000126"
     CDS             complement(43893..44429)
                     /locus_tag="VVE35_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU3060393.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            44562..45452
                     /locus_tag="VVE35_000127"
     CDS             44562..45452
                     /locus_tag="VVE35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU3060394.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            45696..46049
                     /locus_tag="VVE35_000128"
     CDS             45696..46049
                     /locus_tag="VVE35_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU3060395.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46050..47087
                     /locus_tag="VVE35_000129"
     CDS             46050..47087
                     /locus_tag="VVE35_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU3060396.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47133..48140)
                     /gene="galR"
                     /locus_tag="VVE35_000130"
     CDS             complement(47133..48140)
                     /gene="galR"
                     /locus_tag="VVE35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU3060397.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            48304..49482
                     /locus_tag="VVE35_000131"
     CDS             48304..49482
                     /locus_tag="VVE35_000131"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU3060398.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDTLVHTAWAQEE
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49501..50982
                     /locus_tag="VVE35_000132"
     CDS             49501..50982
                     /locus_tag="VVE35_000132"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU3060399.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            51052..51456
                     /locus_tag="VVE35_000133"
     CDS             51052..51456
                     /locus_tag="VVE35_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU3060400.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(51788..53335)
                     /locus_tag="VVE35_000134"
     CDS             complement(51788..53335)
                     /locus_tag="VVE35_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU3060401.1"
                     /translation="MPYAIDLFCGAGGFSEGILQAGFDIIFSSDKSPMVEKTYTNRHQ
                     QLGLVDGVDTHFELADIKELTSDKIFQSINSLKYGNIFERGSVDVIFGGPPCQGFSRL
                     GKRDSKDPRNMLFHEYLRIIKDVMPRYVVMENVTGILDMQMLDFPSVLDDRIYKGQNL
                     VQSILRNELDELGYTLLDVQVLNAANFGVPQQRNRVVFLAYRNDVTPIEYPESDNDIP
                     SVTVYDALGDLYDGEFDYETDYSVQSRLGRTPNTDGQPIINANPLNMEFSRHDDIVTQ
                     RFSLYQQGENRAKAANRIQLEGLELQRDCPNLFTESLFQLNGKTNQPIILKQLKKQDL
                     LQENFQSEKWLQFTNRQLGLLSMNDNNNLRKHHILKSLALRLKTSFEDAELFWNEIKN
                     RLNTEITENQFSNMLRNGELTPAAIEAIFTKKSIRVRLNQDTVSPTMVTLPDDYIHPF
                     FNRVLTVREMARLQSFDDSFIFLGKRTTGGDKRAKETPQFTQVGNAVPPLLAKAIAEQ
                     VMIAINK"
     gene            53524..>53832
                     /locus_tag="VVE35_000135"
     CDS             53524..>53832
                     /locus_tag="VVE35_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3060402.1"
                     /translation="MERYIKENIERLLYSSKLSQSELSKKTGLSRQTINKLLSPNTNY
                     SPKLSTLVVIANALSINFPELLIRTDKISASKEFFSNSVEEYQNILIQNIKLYLRRWN
                     Q"
BASE COUNT        15458 a         9956 c        12119 g        16299 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttgtt tttagagaca ataaaacttc agatccgtaa aacttaattg
     1501 aattactaat ctgaagttat ttttatatcg tttatattat aattgtgtta ttttacttcc
     1561 aactattgag agaaatttcg ccactattta gccagatttc ttttccgtta tcacattgaa
     1621 ttaaaagttt tccattttct gagatgtctt tagcaagtcc cttgtagtct ttttgctcta
     1681 gtgtgaaagt gacttctttt cctagaatga atgactgttt tttgtatagg tataatagct
     1741 cttctgctgg tgtttcgaag aaagcacgcc agatttctat gatcaattca ttccttgtta
     1801 taggagctgt agctttaaat aagctggcag ctttttcttt taattcctga gggaagtctt
     1861 taatagtgaa attgatacct actccaataa tgatatctgt gactaagcca gtttctacag
     1921 aggtcattgc ttcagtaagg attcctccaa ttttatgatt gtttagatag atatcattga
     1981 cccattttat gtcgacatct attaaagtta ggttcttaat ggctttgtag acagctccag
     2041 ctacaagtag tgtgtaggat ggtaatttgt cataggggag atttggttta agatggagtg
     2101 tcatataaat tccaccttgt ggtgagtaga aggaacgttg aaaacggcct cggcctgctg
     2161 tttgatagga agctagatag agggtatttg cttcatggcc taaatcaatt gcttcttttg
     2221 catctagttg tgttgatttt gtttcgggtt taaagctgac tttaattgga agattttctt
     2281 ctagaatctc tggaagaata aggtcaccat tcatcagttt gtatcctcta tttttgatac
     2341 tatcaatttc aatgccttct tgttctagtc gcttgatggc tttccaaatt gatgttcggc
     2401 ttagggatag tttttctgcg attttttctc cgctgatata gtcggtttct ttagatagga
     2461 tttggtagac agcttggtag gatttcataa tgttgccttt ctcactagtt ggtattgaga
     2521 gtattctttt cttgtatgac ttggagtctg attaaagtat tgtttataag ctttcgaaaa
     2581 atggagtgga tctgaaaaac ctaccgagta tgcaattacc ttgatggact cttgggtatt
     2641 ttcgagaagt tgtctagctc ggtgcattcg aacgtagagt aggtattctt tgggtgataa
     2701 ggtattaaat tctttgaata cgcttgataa gtagcttctg tgaacggata gttcttttgc
     2761 taaatcttga attgtaagtg attgaggata gtggctatca attaatcgtt tgcattcaag
     2821 atagagttgg tgggttgatg aaatattctt ttttttctga ttgggagcaa tagttcccag
     2881 atgaaacatc agttcatgaa gttgtcccat gatatggagt tgagctaatt cacttgattt
     2941 tgtaatctga gcgaagcgga caatgtctga gatgagtttt gcagtagtct gggtatgaca
     3001 agtttcagat tggatgagat aggattgatc agaaatttga gaaagagcaa aataatcagg
     3061 ggatttccct ccagtgattc ctaaccagta gtaggcccaa ggttctttac tatctgcttg
     3121 ataaaaggtt agttcctctg gttttaatag aaagaaatct ccttctttta aatcaacaat
     3181 tttacccttg taataaaatt ttccttgtcc tttagtaatg taatgtagaa cgtatgtatc
     3241 acgaatggct ggaccaaaag agtaattagg tgtgcattcc tcatatccat aaaagcttag
     3301 ggcaaggtcg attgttccag tctggtattc tgaaaaaact aacatgtgct acctcctacc
     3361 taacatttta ccatattctt gagacatttt tctattttgg aaagcgattt caggtgataa
     3421 aatataatca ataaagtgat gaggtgaagt aaatgggagt taggatagag aataatctat
     3481 tttatgttga gagtaaaaat ctaagtttga ttattgaaaa tcgaaatggc tacttacttt
     3541 tgaaacattt aggaaagact attaagaact ataaaggttc caatagtgtt tatgaacgag
     3601 accatgcctt ttcaggaaat ccaacggcta ctaatcgaac ctttagttta gatactcagc
     3661 gacagatttt tggacaacat ggcttaggag attttaggaa accaaccata caggttcagc
     3721 atagtgtaac tgaagtaaca gactttcgat ttgtagaagc aaagatttta aaaggtcaga
     3781 atggtccaca gggcttacct tctccacata gcatggacga tacagagact cttgtcttaa
     3841 tgttagaaga ttctaaggct caacttagtc tgactttgta ttatactact tttaataatg
     3901 atgcgactat tgctagctac agtaaattag ataataatag taatcaggaa gttgtcatcc
     3961 ataaagattt ttcttttatg gctgattttc cagctgcaga ttacgaaata gtaactctgc
     4021 agggtgctta tgctcgtgaa aagactgtta gacgtcaaca ggtagaacaa ggaatctttt
     4081 cgattagctc aaaccgaggt gcttctggtc atgctcaaac accagctctt ctactatgcg
     4141 aacaaggagt cacagaggat gctgggaatg tgtttgctat tcaactaatg tatagtggca
     4201 actttgaagc ttttgttcaa aaaaatcaat tgaatgaagt tcgggtggct attggcatta
     4261 atccagaaaa cttttcttgg aagttagctc ctgaggaata ctttgaaaca ccggtagctt
     4321 tagtgaccca ttcagatcag ggattaactg gtattagtca tgaaagtcag aattttgtac
     4381 tgaagcacat tatgctaagt gaattttcta aaaaagaacg tccaattcta atcaataact
     4441 gggaagctac ttactttgac tttcagagag aaaaactgtt agagttagca gatgaagcta
     4501 agaaagttgg cattgaactt tttgtattag atgatggttg gtttggcaat cgttttgatg
     4561 ataatcgtgc tttaggtgat tgggttgtta atgaggaaaa actgggtgga agtctagaaa
     4621 gtctgatttc agctatccat gaaagaggtt tgcagtttgg actttggtta gaacccgaaa
     4681 tgatttctgt agatagtgat ttgtatcgtc aacatcctga ctgggctatt caggttcctg
     4741 gctatgagca tacttattct cggaatcaat tagtacttaa tcttgccaat cctcaggtag
     4801 tagaatactt gaaaagtgtc ttagatcaac tcctatctta tcatgatatt gattacatta
     4861 aatgggatat gaaccgcaat atcactaagc tagggaatgg attaacttat ctagagacac
     4921 agatgcaatc tcatcagtac atgctggggc tttacgaact cgtttcttat ctgacagaga
     4981 agcacagcca tattctcttt gagtcctgct ctggtggtgg tggacgaaat gatcttggta
     5041 tgatgcgcta tttcccacaa gtctgggcta gtgataatac agatgctatt gcacgtttac
     5101 caattcaata cggttcatcc tatctctatc caaccatttc tatgggggct catgtgtcag
     5161 cagtaccgaa tcatcagatg gggcgaacga caccattaga aacacgtggc cttgtagcaa
     5221 tgatgggaaa tttgggctat gaacttgatt tgacaaattt atcagatgaa gagaaagcta
     5281 cgattgctaa tcaggtgaac ttgtataaag aattacgacc agtagttcag ttaggacaac
     5341 agtatagact aattaatcct gatactgcat ccaatgaagc tgctgtacaa tttaattaca
     5401 aaaatcaaac gattgtaacc tacgttcgcg ttttatctgt tgtagagacc atggaaacaa
     5461 ctttaaaatt aaaagatttg gatgaagagg gactatataa attacaggaa aatggcgaag
     5521 tttactcagg tgcagaacta atgtatgcgg gcataactgt tatcttatcc caaggagatt
     5581 ttttgagtag acagtatatt tttagaagac tatgataagg gtgtataaat ttataaagga
     5641 ggctttctaa tggaatggta taaaaaaatc ggacttcttg caactacagc tttagctttg
     5701 tttgggctcg gcgcttgctc caactatggt aaatctgcgg atgacacagt gaccatcgag
     5761 tatttcaacc agaaaaaaga aatgaccaaa accttggaag aaatcactcg tgattttgag
     5821 aaggaaaacc ctaagatcaa ggtcaaagtc gtcaatgtac caaatgctgg tgaagtattg
     5881 aagacacgcg ttctcgcagg agatgtgcct gatgtggtca atatttaccc acagtccatc
     5941 gaactgcaag aatgggcaaa agcaggtgtc tttgaagatt tgagcaacaa agactacctg
     6001 aaacgcgtga aaaatggcta cgctgaaaaa tatgctgtaa acgaaaaagt ttacaacgtt
     6061 ccttttacag ctaatgctta tggaatttac tacaacaaag ataaattcga agaactgggc
     6121 ttgaaggttc ctgaaacctg ggatgaattt gaacagttag tcaaagatat cgttgctaaa
     6181 ggacaaacac catttggaat tgcaggtgca gatgcttgga cactcaatgg ttacaatcaa
     6241 ttagcctttg cgacagcaac aggtggagga aaagaagcaa atcaatacct tcgttattct
     6301 caaccaaatg ccattaaatt gtcggatccg attatgaaag atgatatcaa ggtcatggac
     6361 atccttcgca tcaatggctc taagcaaaag aactgggaag gtgctggcta taccgatgtt
     6421 atcggagcct tcgcacgtgg ggatgtcctc atgacaccaa atgggtcttg ggcgatcaca
     6481 gcgattaatg aacaaaaacc gaactttaag attgggacct tcatgattcc aggaaaagaa
     6541 aaaggacaaa gcttaaccgt tggtgcggga gacttggcat ggtctatctc agccaccacc
     6601 aaacatccaa aagaagccaa tgcctttgtg gaatatatga cccgtccaga agtcatgcaa
     6661 aaatactacg atgtggacgg atctccaaca gcgatcgaag gggtcaaaca agcaggagaa
     6721 gattcaccgc ttgctggtat gaccgaatat gcctttacgg atcgtcactt ggtctggttg
     6781 caacaatact ggaccagtga agcagacttc cataccttga ccatgaacta tgtcttgacc
     6841 ggtgataaac aaggcatggt caatgatttg aatgccttct ttaacccgat gaaagcggat
     6901 gtggattagg agtagagaga ctatgaaaaa agtattacaa aaatattggg catgggcttt
     6961 tgtggtcatc cccctcttgt tacaagcaat tttcttctat gtgccgatgt ttcaaggagc
     7021 cttttacagt tttaccaact ggacaggatt gacttataac tacaaatttg tcggcttaaa
     7081 caactttaag ctcctcttca tggatccaaa attcatgaat gcgattggct ttaccgcaat
     7141 cattacgatt gccatggtgg ttggtgagat tgcactcggg atcttcattg cgcgtgtctt
     7201 gaattctaaa atcaaaggcc aaaccttctt ccgtgcttgg ttcttcttcc cagctgtttt
     7261 atctggtttg acagtggctt tgatcttcaa gcaagtcttc aactacggtc ttccagcgat
     7321 tgggaatgcc cttcatattg aatttctcca aaccagtctt ttagggacta agtggggagc
     7381 aatctttgcg gctgtctttg tccttctttg gcaaggggtg gctatgccca tcatcatctt
     7441 cctagctggt ttgcaatcta ttccaactga gattacagag gcagcaagga ttgatggtgc
     7501 gacgagcaag caagttttct ggaacattga attgccttac ttgctaccaa gtgtctctat
     7561 ggtctttatc ctagccctaa aaggtgggct gactgccttt gaccaagtct ttgccatgac
     7621 cggtggtggt ccaaacaatg ccacaacctc acttgggctc ttggtttata actatgcctt
     7681 taaaaacaac caattcggtt atgccaatgc cattgccgta atcttgttct tcttaattgt
     7741 agtgatttcg atcatccaat tgagagtatc taagaaattt gaaatttaag aggagaagca
     7801 tgatgaaaca agatgaaaga aaagccctga ttggcaaata cattctattg attctaggat
     7861 cggttctgat tttagtgccg ctccttgcta ccctctttag ttccttcaaa cccactaagg
     7921 atattgtaga taatttcttt ggctttccaa ccaacttcac atgggacaac tttagccgtc
     7981 tcttagctga tgggattgga ggctattatt ggaactctgt cgtcatcact gtcttgtctt
     8041 tacttgcagt aatgatcttt atacctatgg cagcctactc catcgctcgc aatatgagta
     8101 aaagaaaagc ctttaccatc atgtataccc tcttaatcct cggaatcttc gtacctttcc
     8161 aagtcatcat gattccgatt acggttatga tgagtaaact cggtttggct aatacctttg
     8221 gtttgatctt actctacttg acctatgcga ttccacagac cctctttctc tatgttggct
     8281 atatcaaaat ctcgattcca gaaagtctgg atgaagcagc agagatcgat ggggctaatc
     8341 aatttacaac ctatttccgc atcatcttcc caatgatgaa accgatgcat gcgacaacca
     8401 tgatcatcaa tgccctttgg ttctggaatg acttcatgtt gccactcctt gtcttgaacc
     8461 gggattccaa aatgtggact ctgcctttgt tccaatacaa ctacgcaggc caatatttca
     8521 acgactacgg accaagcttt gcctcttacg tggtcggcat tatcagtatc accattgtct
     8581 atctcttctt ccaacgccat atcatttcag gaatgagcaa cggggcagtg aagtaatact
     8641 caatgaaaat taaagagcaa actaggaagc tagccgcagg ttgctcaaaa cactgttttg
     8701 aggttgtgga tgaaactgac gaagtcagta accataccta cggtaaggca acgctgacgt
     8761 ggtttgaaga gattttcgaa tagtataaga agagctcatg gaatctatag tctttgacac
     8821 agaggaagag ttcagcttgt tgataattct atgtagaaaa caagaaagta gtactttgtt
     8881 tatcacagtc tatattagta cgagtttgat gataaataga gatttaaaat gaattttatt
     8941 tatgaagcca gatcatatga gacttggctt caattagaaa aaggagagta atgatgccaa
     9001 ttcaaaataa aaccatgttg attacctatt ctgatagcct tggaaataat cttaaagact
     9061 tatatgataa tttggaagag cattttggag atgctattgg aggagttcac cttttaccat
     9121 ttttcccatc aacaggtgat cgtggatttg cgccagttga ctacgacgaa gtggactcag
     9181 cttttggtga ttgggaggat gttaagcgtt taggtgagag atattatctt atgtttgatt
     9241 ttatgattaa tcatatttct cgtcaatcca agtattataa ggactatcaa gaaaaacatg
     9301 aagccagtga atttaaagat ctctttttaa actgggataa gttttggcca gaaaaccgtc
     9361 cgacacagtc tgatgtagat ttaatttaca agcgtaagga tcgtgcacca aagcaagaga
     9421 ttgtgtttga agatggttca gtggaacatt tgtggaatac ctttggtgag gagcagattg
     9481 atcttgatgt gaccaaagaa gtaactatgg aatttatccg taagaccatt cagcacttgg
     9541 caagtaatgg gtgtgatttg attcgtctag atgcctttgc ttatgcagtg aagaaattgg
     9601 atactaatga tttctttgtg gaaccagata tttgggattt attggacaaa gttcgagata
     9661 tcgctgctga gtatgggaca gagcttttac ctgagattca tgaacactat tcgattcagt
     9721 ttaaaatagc agaccatgat tactatgttt atgattttgc tcttccaatg gtgacacttt
     9781 atactcttta cagttccaga acagagcgtt tggctaagtg gttaaagatg agcccgatga
     9841 agcaatttac gacgctagat acccatgatg ggattggagt agtagatgtc aaggatatcc
     9901 tgaccgatga ggagattgac tatgcttcaa atgaactcta taaggttgga gccaatgtca
     9961 aacgtaagta ctctagtgcc gagtataaca acttagatat ctaccaaatc aattcaacct
    10021 actattcagc gcttggagat gatgatgtca agtattttct cgctcgtcta attcaagctt
    10081 ttgccccagg tattcctcag gtttactatg tgggtctatt agcaggcaag aatgacttga
    10141 aattattaga agaaactaaa gaaggtcgaa atattaatcg tcattactat agcaacgagg
    10201 aaatagcaaa agaagtgcaa cgacctgttg tgaaggccct tctcaatcta ttttctttcc
    10261 gtaaccgttc agaagccttt gatctagaag ggactactga gatagagaca ccaacagctc
    10321 atagcattgt aatcaaacgt caaaataaag ataagtccgt aacagcagta gtagaaattg
    10381 atttgcaaaa tcagacttat cgggtaattg agaatggagt tgaagtatca ttttgaagcc
    10441 ttgatatggt ggataaaata gggtttttat tttggaaaac gtttcctttg ttttcaaatt
    10501 gctaaaaaag tggtacaata taggatagct tactattatc tgaatcagct gatttggaga
    10561 gaaaggattc attttgaaat caataggctt tattgaaaag ctgaaggggt tgtctagtaa
    10621 agagctgatt ttattgggaa ttatcctaag tatcttttta cccttttatc tttttgtagt
    10681 tgtactctgt ttatatatta tcagtttgat ttttacagga gacatgaaaa gtattcttca
    10741 gaaaatgggg gagcatccga tgctgcttct ttttcttagc tatagtactg ttatatccat
    10801 tcttgcacaa aattggatgg gtcttgtggc ttcagtagga atgtttctat ttactatttt
    10861 ctttttgcac tatcagtcga ttttatccca taaattcttt cgattgattt tgcagttcgt
    10921 cttgtttggt agtgtcttgt cagctgcttt tgccagttta gaacatttcc aaattgtgaa
    10981 gaaatttaac tatgcttttc tttcacccaa tatgcaggtg tggcatcaga accgggcaga
    11041 agtgaccttc tttaatccta attattatgg aattatttgt tgtttctgta ttatgattgc
    11101 tttctatctg tttacaacga ccaagttgaa ttggttgaaa gtattctgtg tgattgcagg
    11161 ctttgttaat ctctttggtt tgaactttac tcaaaatcga actgcctttc ctgctattat
    11221 cgctggagca attatctatc tctttacgac tattaaaaac tggaaggcct tttggcttag
    11281 tattggggtc ttcgcgattg gtttgagttt cctcttttct agtgatttgg gagttcgaat
    11341 gggtacttta gactcttcta tggaagaacg catttctatc tgggatgctg ggatggcctt
    11401 gtttaagcaa aatccttttt ggggtgaagg gccattgacc tatatgcact cttatcctcg
    11461 gatacatgct ccttatcatg aacatgccca cagtctttat attgatacga ttctgagtta
    11521 cggaattgtg ggtaccattt tattagtttt gtcttctgtt gctcctgttc gcttgatgat
    11581 ggatatgagt caggagtcgg ggaaacgtcc gattatcggc ctttatctat ctttccttac
    11641 agtggttgct gtgcacggaa tttttgactt ggctctcttc tggattcagt caggctttat
    11701 tttcttgcta gttatgtgca gtattccatt ggagcatcga atgttggtat cggacatgac
    11761 ggattaagtt ttataagttt gaaatcttct accttgggta ggagattttt ttatacgatg
    11821 ggcatgtcgt gcatctgagg tgtaagtcct ctgtagggat ccactaccgg tgaatacaat
    11881 agcgctcccc aagcctgact gtcgtgaggt agcggattaa aatggtctgg ggatagatcg
    11941 ttttaagtct gacgcaggaa ataagaattg tcagagaaag caatagtgaa atcgtaggct
    12001 atgaatagaa acgtgatagc gaggagcata tagtggataa ggtagcgaga aattttaaag
    12061 tataaaagat agtcttaatc tatatatgta aatcacaaaa gtaattgaat tcggaataag
    12121 gttggtgtga aaagacatag taaactgaat cttgaatact agaatgatac ttctacaata
    12181 ttgttataat ttaatttgaa ttccaaaatc acattgttcc gtccgtattt cattttactg
    12241 tatgtcttcc taaactccaa agtttctgcg tcaaatttcc tattttcact gtagtctttg
    12301 acacttcgaa cgacaatttg aatcatatat tcaaaaaaaa tgaaaaattt gtgccaacaa
    12361 gcctaaatat tctgaaaatt tgtttgattt ttaatggata atgtgatata atggttgtca
    12421 atctataatt cacataaaca gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag
    12481 caggtcttgt tttattagca gcaggtgtac ttgcagcatg cagttcttca aaatcatctg
    12541 attcatcagc ccctaaagct tatggctatg tttatacagc agacccagaa accttggact
    12601 acctgatttc aagtaaaaat agtacaacag tagtgacttc aaatgggatt gatggtttat
    12661 tcactaacga taattacggt aatcttgctc ctgcagttgc agaggattgg gaagtctcta
    12721 aggatggttt gacctacact tataagattc gtaaaggggt taaatggttt acctctgatg
    12781 gagaagaata tgcagaggtg acggctaaag atttcgtgaa cggtttaaaa cacgcagcag
    12841 ataaaaaatc agaagctatg tatttagctg aaaattcggt taaaggcttg gcagattatc
    12901 tatcaggaac ttcaacagat ttttcaacag ttggtgtcaa ggcggttggt gattatacgt
    12961 tacaatacac tttgaaccag cctgaaccgt tctggaactc taagttgacc tattctattt
    13021 tctggcctct gaatgaagaa ttcgaaacat caaaaggaag cgattttgct aaaccaacag
    13081 atccgacatc cttgctttat aatggtccat tcttgttgaa agggttgact gcaaaatctt
    13141 ctgtagagtt tgtaaaaaat gagcaatatt gggataaaga aaatgtccac ctagatacta
    13201 tcaatctagc ttactatgat ggatcagatc aggagtcgct agagcgtaac ttcactagtg
    13261 gagcttatag ttatgcccgt ctttacccta ccagctccaa ctattctaag gttgcagaag
    13321 aatacaagga caatatctat tacacacaat caggctctgg gattgctggt ctgggtgtga
    13381 atattgatcg ccaaagttac aactatactt ctaaaactac agattcagag aaagtagcta
    13441 ctaagaaggc attgcttaac aaagatttcc gtcaagcctt gaattttgct cttgatcgct
    13501 cagcttactc agctcaaatc aatggtaaag atggagcagc tttagcagtt cgtaatttat
    13561 ttgtaaaacc agactttgtt tcagctggtg agaagacctt tggtgattta gtcgctgctc
    13621 aacttcctgc ttatggtgat gagtggaaag gtgtgaattt agctgatggg caggatggtt
    13681 tattcaatgc tgacaaggcc aaggcagagt ttgcgaaagc taagaaagct ttagaagcag
    13741 acggcgttca gtttcctatt catctggacg ttccagtaga ccaagcatca aaaaactaca
    13801 tatctcgtat tcagtccttt aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg
    13861 ttgttgatat tcaacaaatg acaagtgatg aattccttaa tattacttac tatgctgcca
    13921 atgcttcatc tgaggattgg gatgtatcgg gaggagtttc atgggggcca gactatcaag
    13981 acccatctac ttacctggat attttaaaaa caactagcag tgaaactaca aaaacatatt
    14041 taggatttga taatccaaat agcccttcag tagttcaagt tggtttgaaa gaatacgata
    14101 aattagttga tgaagctgcc aaagagacaa gcgacttgaa tgtccgttat gaaaaatatg
    14161 cagcggctca agcatggttg acagatagtt cactctttat tcctgctatg gcttcttctg
    14221 gtacagcacc agtgctttca cgaattgttc catttactgg agcttctgcg caaacaggct
    14281 ctaaggggtc agatgtttac ttcaaatatt tgaaattaca agataaagtg gtgactaagg
    14341 aagagtatga aaaagctcgt gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag
    14401 ctcaaaaaga attggcaagt catgtgaagt aaataatttc taatagaact ttctctccat
    14461 gaggagaagg ttcttttggg atttttaaag gaaataatat gaaaaaatat atttttatgc
    14521 gtgttttgcg gtcattggtt tcgattttct tagtaacgac tttgacctac acgattatct
    14581 ataccttggt tcctcgaaaa ttgattttca agcaggatcc taactataat aaaattgcga
    14641 caacggctga taaacgtgat aactatgaaa atactgtgtt tgagcgtatg ggctacattg
    14701 agtattacga tactaaagag ttgcaagaaa aggcaagtag catggattct tctgtaacag
    14761 tagaagcaaa tgcgaccaat aaagctattt atgaaaagta catcaatcaa ttaggtcatg
    14821 gttggacttt gggagaattt actgaaagtg gtcaattcta tgctactcgt gaaattccaa
    14881 tttttgaacg tgtttttcac ttctatgcta acttgattga tattgatcat acaaataaaa
    14941 tccaagaccc tgaaaatcca gacttgaaac gctaccttcg ttttgaaaat gatccagcta
    15001 tcggatggtc attggtcggt tcaggaacta aacataaata tctcttgtac tttaacagtc
    15061 agttcccatt tgttcatcaa aactttgtga acttgaattt aggtgactct tacccaacct
    15121 atgctaatac accagttctt caggttatta ctcaaggtca aggacaaacc aaaactgccc
    15181 aagttcagtt cccaacaggt aagaaaacgt cttctgtaaa tatttactca agaacctaca
    15241 agtcacctag tcaggctgac tctcgtgaag tagctagcta tgggaaagat gatccttata
    15301 cagcgactga aagtaattac caatatccat ctatgattgt cagctctgct attactggtt
    15361 tgattggttt ggttcttgcc tatgctcttg ccgtgccact tggttcagcc atggctcgtt
    15421 tcaagaacac ttggattgat agcctctcaa cagggacttt gaccttcttg cttgctcttc
    15481 caacgattgc cttggtttac atcgttcgat tgattggatc atctattgcc cttccagatt
    15541 cattccctat cttgggagct ggagattggc gttcttacgt tttaccagca gtcatccttg
    15601 gtttgttggg tgctcctgtt acagccattt ggattcgtcg ttacatgatt gacttgcaat
    15661 ctcaagactt tgttcgtttc gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca
    15721 aacacatctt taaaaatgcc atggttccgc tggtttcagg aattcctggt gccattattg
    15781 gggttatcgg tggtgcaacc cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa
    15841 tgttgattga ctctgtaaaa gcatctaata actctatggt cgttggtctt gtcttcatct
    15901 ttacatgtat ttctatcttc tcacgtcttt tgggagatat ttggatgact attattgacc
    15961 cacgtattaa attgactgag aaaggaggca aataatgtct acaatcgata aagaaaaatt
    16021 tcagtttgta aaacgtgacg attttgcctc tgaaactatt gatgcgccag catattctta
    16081 ctggaaatca gtgtttaaac aatttatgaa gaaaaaatca actgtagtca tgttgggaat
    16141 cttggtaacc atcattttga taagtttcat ctacccaatg ttttctaagt ttgatttcaa
    16201 tgatgtcagc aaggtaaacg actttagtgt tcgttatatc aagccaaatg cggagcattg
    16261 gttcggtact gacagtaacg gtaaatcgct ctttgacggt gtctggttcg gagctcgtaa
    16321 ctccatcctc atttctgtga ttgcgacagt gattaacttg gttatcggtg tttttgtcgg
    16381 tggtatttgg ggtatttcaa aatcagttga ccgtgtcatg atggaagttt acaacgtcat
    16441 ctcaaacatc ccacctcttt tgattgttat tgtcttgact tactcaatcg gagctggatt
    16501 ctggaatctg atttttgcca tgagcgtaac aacatggatt ggtattgcct tcatgatccg
    16561 tgtgcaaatc ttgcgctatc gtgacttgga atacaacttg gcgtcacgta ctttgggaac
    16621 accaaccttg aagattgttg ccaaaaatat catgcctcaa ttggtatctg ttattgtgac
    16681 aaccatgact caaatgcttc caagctttat ctcatacgaa gccttcttgt ctttcttcgg
    16741 tcttggatta ccgattacag tgccaagttt gggtcgtttg atttcggatt attcacaaaa
    16801 cgtaacaacc aatgcttact tgttctggat tccattgaca acccttgtct tggtatcctt
    16861 gtcccttttc gtagttggtc aaaacttagc ggatgctagt gatccacgta cacatagata
    16921 ggagtagaaa tgacaaaaga aaaaaatgta attttgactg ctcgcgatat tgtcgtggaa
    16981 tttgacgttc gtgacaaagt attgacagcc attcgcggcg tttcccttga actagttgaa
    17041 ggagaagtat tagccttggt aggtgagtca ggatcaggta aatctgtttt gacaaagacc
    17101 ttcacaggta tgctcgaaga aaatggtcgc attgcccaag gtagtattga ctaccgtggt
    17161 caggatttga cagctttatc ttctcacaag gattgggaac aaattcgtgg tgctaagatt
    17221 gcgactatct tccaggaccc aatgactagt ttggacccca ttaaaacaat tggtagtcag
    17281 attacagaag ttattgtaaa acaccaagga aaaacagcta aagaagcgaa agaattggcc
    17341 attgactaca tgaataaggt tggcattcca gacgcagata gacgttttaa tgaataccca
    17401 ttccaatatt ctggaggaat gcgtcaacgt atcgttattg cgattgccct tgcctgccga
    17461 cctgatgtct tgatctgtga tgagccaaca actgccttgg atgtaactat tcaagctcag
    17521 attattgatt tgctaaaatc tttacaaaac gagtatcatt tcacaacaat ctttattacc
    17581 cacgaccttg gtgtggtggc aagtattgcg gataaggtag cggttatgta tgcaggagaa
    17641 atcgttgagt atggaacggt tgaggaagtc ttctatgacc ctcgccatcc atatacatgg
    17701 agtctcttgt ctagcttgcc tcagcttgct gatgataaag gggatcttta ctcaatccca
    17761 ggaacacctc cgtcacttta tactgacctg aaaggggatg cttttgcctt gcgttctgac
    17821 tacgcaatgc agattgactt cgaacaaaaa gctcctcaat tctcagtatc agagacacat
    17881 tgggctaaaa cttggcttct tcatgaggat gctccaaaag tagaaaaacc agctgtgatt
    17941 gcaaatctcc atgataagat ccgtgaaaaa atgggatttg cccatctggc tgactaggag
    18001 gaaggaaatg tctgaaaaat tagtagaaat caaagattta gaaatttcct tcggtgaagg
    18061 aagtaagaag tttgtcgcgg ttaaaaatgc taacttcttt atcaacaagg gagaaacttt
    18121 ctcgcttgta ggtgagtccg gtagtgggaa aacaactatt ggtcgtgcta tcatcggtct
    18181 aaatgataca agtaatggag atatcatttt tgatggtcaa aagattaatg gtaagaaatc
    18241 gcgtgaacaa gctgcggaat tgattcgtcg aatccagatg attttccaag accctgccgc
    18301 aagtttgaat gaacgtgcga ctgttgatta tattatttct gaaggtcttt acaatcaccg
    18361 tttatttaag gatgaagaag aacgtaaaga gaaagttcaa aatattatcc gtgaagtagg
    18421 tcttcttgct gagcacttga ctcgttaccc tcatgaattc tcaggcggtc aacgtcaacg
    18481 tatcggtatt gcccgtgcct tggtcatgca accagacttt gttattgcag atgagccaat
    18541 ttcagccttg gacgtttctg tacgtgccca agtcttgaac ttgctcaaaa aattccaaaa
    18601 agagctcggc ctgacctatc tcttcatcgc ccatgacttg tcggttgttc gctttatttc
    18661 agatcgtatc gcagttattt acaagggtgt tattgtagag gttgcagaaa cagaagaatt
    18721 gtttaacaat ccaattcacc catatactca agccttgctt tcagcggtac caatcccaga
    18781 tccaatcttg gaacgtaaga aggtcttgaa ggtttacgac ccaagtcaac acgactatga
    18841 gactgataag ccgtctatgg tagaaatccg tccaggtcac tatgtttggg cgaaccaaac
    18901 cgaattggca cgttatcaaa aaggactaaa ctagtaatgg ttttataatt tccatgtcaa
    18961 tttttatgga aattaggctc tttgtcaact gtagtgggtt gaagtcagct aagctcgaga
    19021 aaggacaaat ttcgtccttt cttttttgat gttcaaagcg ataaaaatcc gttttttgaa
    19081 gttttcaaag tttcgaaaac caaaggcatt gcgcttgata agtttgatga gattattggt
    19141 cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttgataatct tttctttatc
    19201 tttgaggaag gttttaaaga cagtctgaaa aataggatga acctgcttaa gattgtcctc
    19261 gataagttcg aaaaatttct ctggttcctt attctggaag tgaaaaagca agagctgata
    19321 gagctgatag tggtgtttca agtcttgtga ataactcaaa agcttgttta aaatctcttt
    19381 attggttaaa tgcatacgaa aagtaggacg ataaaatcgc ttatcactca gtttacggct
    19441 atcctgttgt atgagcttcc agtagcgctt gatagccttg tattcatggg attttcgatg
    19501 aaactgattc atgatttgaa cacgcacacg actcatagca cggctaagat gttgcacaat
    19561 gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa acagtctggg agactgtttc
    19621 agcctgagcc tagaaatttg aaagcgaagc tgtttagcca agccatagta agggctaaac
    19681 atatccatag taatgatttt gacgcgacat cggacggctc tatcgtagcg aagaaagtga
    19741 tttcgaatga tagcttgtgt tctgccttca agaacagtga tgatatcgag cttttcaaaa
    19801 tcttgcgcaa taaagctcat ctccatctcc cgattgaaac agtcactccc cggactgttt
    19861 caacgtccca agacataatc ttaggcagat ttctaaaatt acactcaaag tggaagtcat
    19921 tgagctttcg aatgacagtt gaagttgaga tggaaagctg atgggcaata tcagtcatag
    19981 aaatcttttc aattaacttt tgagcaatct tttggttgat gatacgaggg atttgatgat
    20041 tcttcttgac gatagaagtt tcagcaacca tcattttaga acaatgatag cacttgaaac
    20101 gacgctttct aaggagaatt ctagtaggca taccagttgt ttcaagataa ggaattttag
    20161 aaggtttttg aaagtaatat ttcttcattt gacttccgca ctcaggacaa gatggagctt
    20221 catagtccag tttggcgatg atttccttgt gtgtatcctt attgatgatg tctaaaatct
    20281 ggatattagg gtctttaatg tctagtaatt ttgtgataaa atgtaattgt tccatatgat
    20341 tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac
    20401 acaaaatagg ctccataata tccatagggg ggttacccac tacaaatatt atagagccga
    20461 aaaaggctga gatctggtgt ctcagtcttt tatttcttgc gttttgcaaa atctacaaat
    20521 ctaaatttct ctaacttatg gcgactttcg gtaaactgaa attggcgtcc gtcttggaga
    20581 tagacttttg atttaatcga aacgacataa gggtctttgc caatgtccat gagaatcttg
    20641 tctcggtcgc ttgagtggtc aatggtgatt tccttctgag cataatcaat aaggagtttg
    20701 aggccatttt ctatataaga atagatagac tgctcagcaa tttggcgagt gagatttgga
    20761 attagttcca tatccagata gtccgtatcc agaacggata ccagatcatc caccacacgc
    20821 tggcggacca ccttccaaac catccgaaac tctgggaaac cggttatcag tgaggatttt
    20881 ttatcaataa taatcttgtc cagactgacc acgttggttt tagagcgcag tccaagttct
    20941 ttaactagtt cttggtagct ggttaggttg gatacaggga aattgacggt ttcttctttg
    21001 acgacttgag aaccttgccc tcttatcttt ttgatcaatc cttcctcttg gaggagagac
    21061 agggcctttc ggacggtatc acgactcact tgatattgct ccataaggtc gtgctcgcta
    21121 ggaaggaaat ctccgacagc gtaagtctcg ttttgaatgg tttcttggat ttgcttaaat
    21181 aattgttggt atttcttcat tttgcctgct taaataggtt tttagtctag tttaatgaac
    21241 acctgtatct attttaccat acaagtgcta gaaaatttac aaaaaaacac cctttttata
    21301 ccaaacaaat tgcagacaag tttggtatcg tttacaatat acttatcaaa tcaaacttgc
    21361 tttgacaagt ataaggagaa tcaaatggga aaatttgaac aagaagccaa agatctgctt
    21421 caggcaattg gaggcaaaga aaatgtgact gccgtaactc actgtgcgac acggatgcgc
    21481 tttgttttag gagatgataa gaaggctaat gttaaagcta tcgagtcaat tccagctgtt
    21541 aaaggaactt ttacaaatgc aggtcaattt caggtaatca ttggaaatga cgtgcccatc
    21601 ttttataatg attttacagc cgtttcaggt attgagggtg tttccaaaga agcaaccaag
    21661 tctgcagcta agagtaatca aaacgtggtt caaggtgtta tgaccactct ggcggaaatc
    21721 tttactccga ttattccagc cttgattgtt ggtggattga tcctcggttt ccgtaatgtc
    21781 ttggaaggtg tccattggtc gatgttggat ggcaagacca tcacagaatc ctctcagttt
    21841 tgggcagggg ttaatcactt cctctggttg cctggtgaag ctatcttcca gttcttacca
    21901 gtagggatta cttggtctgt ttctcgtaag atgggaacca gccaaatttt gggaattgtt
    21961 ctcggaatct gtttggtatc gcctcagttg ctcaatgcct atgcggttgc ttcaacgcca
    22021 gcagctgata tcgcggcaaa ctgggtttgg aattttggtt attttactgt taatcgtatt
    22081 ggttaccaag cccaagttat cccagccttg cttgcaggtt tgagtctgtc ttatcttgaa
    22141 attttctggc gcaaacatat cccagaagtc atttccatga tttttgtacc ttttttgtca
    22201 ttgattccag ccttgatttt ggctcatact gttttgggac caatcggttg gacaattgga
    22261 caaggacttt catcagttgt cttggcaggt ttaactggtc cagttaaatg gctcttcggt
    22321 gcaatttttg gtgccctcta cgctccattt gtcatcacag gtctgcacca tatgaccaat
    22381 gccattgata cacaattgat tgcggatgct ggtggcactg ccctatggcc aatgattgct
    22441 ctttctaata ttgctcaagg ctcagccgtg tttgcctatt atttcatgca tcgccatgat
    22501 gagcgtgagg ctcaggtttc acttcctgca accatttcag cctatctcgg tgttacagaa
    22561 ccagctcttt ttggggttaa cgtaaaatat atttatccat ttgttgctgg gatgactggt
    22621 tcagcccttg caggcatgtt atccgttact tttaatgtaa ctgcggcttc tattggtatc
    22681 ggtggtttgc caggtattct ctctattcaa cctcaataca tgctgccatt tgcaggaact
    22741 atgctagttg cgattgttgt tccaatgctc ttgactttct tctttcgcaa ggctggtctc
    22801 ttcacaaaaa tagagggcga tacgaacttg caggcagaat tcgttgctca agaagaagca
    22861 gaatttgtga gccatgaacc agtagaactt acttcggtag aaattatcag cccactaact
    22921 ggccaagtga aagaattgag tcaagcgacg gatcctgttt ttgcatcagg tgtcatgggg
    22981 caaggtctgg tcattgaacc aagccaaggt gagttgacct ctccagttaa tggaacagtg
    23041 acggttcttt tccctaccaa gcatgccatc ggcattgtct ctgacgaggg agttgaattg
    23101 ctcatccaca tcggtatgga tacagtaggt cttgatggca aaggttttga aagtcttgta
    23161 gtccaaggag atcacgttat agttggccag caactgattc gttttgatat ggatgtcatt
    23221 aaggctgcag gtctggtgac agaaactctt gttatcatca ccaaccaaga tgcttataca
    23281 gcgactattc ccggaactta tccgacaacg atccaagctg gagcatctct catggtcgct
    23341 acacgaatct aatactcttc gaaaatctct tcaaaccacg tcagctctat ctgcaacctc
    23401 aaaatagtgt tttgagcaac ctgtggctag cttcctagtt tgttctttga ttttcattga
    23461 gtataattga aatagcacaa atagggacgg aagactgctt cctccctttt atcagaaaag
    23521 gagaaacaga tgacacttga taaaggaaaa gtagtctatc aaatctatcc aaaatcttac
    23581 aaagacacca ctgaaaacgg tattggggat ttccgtggga ttatcgaaaa aattccctat
    23641 ttagccaaac taggtgtgga tatggtctgg ctcaatccat tctatccaag ccctcaacgg
    23701 gataatggtt acgatatttc agattatatg gcagtggatc ctctttttgg tgatatggct
    23761 gattttgagg aaatggtgtg tgtcggtaaa gagcacaaga ttgactttat gctggacatg
    23821 gtacttaatc attgttcgac agagcatgaa tggtttcaga aagccctagc tggtgataag
    23881 tattatcaag actttttctt catccaagac caaccaacag attggcagtc taagtttggt
    23941 ggttctgcat gggcgccttt cggggatact gggaaatact atcttcacct atttgatgag
    24001 acccaggctg accttaactg gcgcaattcc aatgtccgta aggagctttt caaggttgtt
    24061 aatttctggc gcaacaaggg tatcaaaggt ttccgatttg atgtgatcaa tttgatcggt
    24121 aaggacgagg tttcagtgga ctgtcctgaa aatgaaggga agccagctta tacagacaaa
    24181 cccatcgttc ataactattt gcgtatgatg aaccaagcca cttttggatc tgacgatagc
    24241 tttatgacag ttggggaaat gtcttctacc accatggaaa actgtgtcct ctattcgtcg
    24301 cctgatcgtc aggaattatc catgaccttt aattttcatc acctcaaggt ggactataag
    24361 gatggacaaa agtggacctt ggctcccttt gattttgaag agttgaagag tctttaccat
    24421 agttggggta aggaaatgag tgataaagac ggttggagtg ccctattttg gaacaatcac
    24481 gatcaaccac gtgccttgaa ccgttttgtc gatattcaaa acttccgcaa ggaaggagcc
    24541 accatgctag cagccagcat tcacctgtca cgtgggacac cttatatcta catgggtgag
    24601 gaaatcggga tgattgaccc agactatgat tctatggctg attatgtgga tgtcgaatcg
    24661 cttaatgcct atcagatgct cttagaagaa ggaaagagtc agcaagaagc cttccagatt
    24721 attcaagcta agtcgcgtga taattcacgc attcccatgc agtgggatgc ttcggaaaat
    24781 gcaggatttt caacaggcac tccttggttg aaagctggta aatcctataa atacatcaat
    24841 gtagaaaatg aaatccaggg tccgattttt accttctatc aagacttgat tcgactgcgt
    24901 aaggaaatgc caatcatttc agaaggaagt tacaaaccag cctttgaaga tagcaaacaa
    24961 gtctacgctt ttgaacgcca gtttgaggat caaaagttac tagtgctcaa taacttttat
    25021 gctaaagaag tggaaatcga cttgccagca gtctaccaaa atggacagat tttgatatca
    25081 aactatgaag atgcagaagt gtctgagaaa attctactca aaccctatca aacactggct
    25141 atttatgtaa attaatactc ttcgaaaatc tcttcaaacc gtgtcagttt tatctgcaac
    25201 ctcaaagcgg tgctttgagc aacctgtggc tagcttccta gtttgctctt tgattttcat
    25261 tgagtataaa cttagcaagg agacctcttt ttcagaaaag cagggttctt tttgctgtct
    25321 attccatccc tgaaagtgta gaacaaactg gtcagggtgg ctagtttgtg gtataatgaa
    25381 agggactcaa aaagaaacag gagaaaaatg atggatttac ttttagcaat tgtattgatt
    25441 gtgctagctt ttctaggagg agctcttgga ggaatgtact tggttcgtaa gcaaatcgaa
    25501 aaagaatttg ctgacaaccc acgtttgaat gctgaagcag ttcgtactct tttgagtgca
    25561 aatggtcaaa aaccaagcga agctaaggta caacaagttt accaccaaat catccgccaa
    25621 caaaaggcag cccttgctaa caataaaaag aaaaaataaa taaggaaaag tctgggatga
    25681 aagttccaga cttctcacta tgttggttat ggtttaggga tgagactttt tccttgcatt
    25741 ctattgatat ttgattatga ttaagagaga ggtagttgac attctatcaa gctgtcggtc
    25801 agttcgttca gtacaaggaa tcataatgaa cgatcaatca gaaaaaagac tagaaagaag
    25861 actgtatgga taatcgacca attggttttt tggattcggg tgtcgggggc ttgaccgttg
    25921 tgcgcgagct catgcgccag cttccccatg aagaaatcgt ctatattgga gattcggcgc
    25981 gggcgcccta tggcccccgt cctgctgagc aaattcgtga atatacttgg cagctggtca
    26041 actttctctt gaccaaggat gtcaaaatga ttgtcattgc ttgtaacact gcgactgcgg
    26101 tcgtctggga agaaatcaag gctcaactag atattcctgt cttgggtgta attttgccag
    26161 gagcttcggc agccatcaag ttcagtcaag gtgggaaaat cggagtgatt ggaacgccca
    26221 tgacggtaca atcagacata taccgtcaga aaatccatga tctggatccc gacttacagg
    26281 tggagagctt ggcctgtccc aagtttgctc ccttggttga gtcaggtgcc ctgtcaacca
    26341 gtgttaccaa gaaggtggtc tatgaaaccc tgcgtccctt ggttggaaag gtggatagcc
    26401 tgattttggg ctgtactcat tatccactcc ttcgccctat tatccaaaat gtgatggggc
    26461 caaaggttca gctcatcgat agtggggcag agtgcgtacg ggatatctca gtcttactca
    26521 attattttga aatcaatcgt ggtcgcgatg ctggaccact ccatcaccgt ttttacacaa
    26581 cagccagtag ccaaagtttt gcacaaattg gtgaagaatg gctggaaaaa gagattcatg
    26641 tggagcatgt agaattatga caaataaaat ttatgaatat aaggatgacc aggactggta
    26701 tgttgggtct tatagtattt ttggtggcgt taacagtttg agcgactatc agacagattt
    26761 tcctctgttt gaattctcca aaatatttgg agatgaagag tatggtttcc cgctttcagt
    26821 tactgtttta cgctatggtt ctacctaccg tttgttctcc tttgtggtag atatgcttaa
    26881 tcaagaaatg ggacgaaact tggaagttat tcaacgtcat ggggccctgc tcttggttga
    26941 aaatgggcaa ctcttgtatg tagaattgcc taaagaaggg gtcaatgttc atgatttctt
    27001 tgagacaagc aaggtcagag aaaccttgtt gattgcgact cgtaacgaag gcaaaaccaa
    27061 ggaattccga gctatctttg ataagttagg ctacgatgtg gaaaatctta atgactaccc
    27121 tgacctgcct gaagtagcag aaacaggtat gacctttgaa gaaaatgccc gccttaaggc
    27181 agaaaccatt tctcaattaa cgggcaagat ggttttggca gatgattctg gtctcaaagt
    27241 cgatgtcctt ggtggcttac caggcgtctg gtcagctcgt ttcgcaggtg tgggagcaac
    27301 tgaccgtgaa aataatgcca aactcttgca cgaattggcc atggtctttg aactcaagga
    27361 ccgctcggct cagttccaca caaccctagt cgtagccagc ccaaataagg aaagtttagt
    27421 tgttgaagca gactggtcag gttatattaa ctttgaacct aagggtgaaa atggctttgg
    27481 ctatgatccc ctcttccttg taggagagac aggtaagtca tcagctgaat taaccctgga
    27541 agaaaaaaat agtcaatctc accgtgcctt ggccgttaag aaacttttgg aggtatttcc
    27601 atcatggcaa agcaaaccat cattgtaatg agcgattccc atggcgatag cttgattgtg
    27661 gaagaagtcc gtgatcgcta tgtgggcaaa gtcgatgccg tttttcataa cggcgattct
    27721 gaactacgtc cggattctcc actttgggag ggcatccgcg ttgttaaagg gaacatggac
    27781 ttctacgccg gctacccaga acgtctggtg actgagcttg gttcgaccaa gattatccaa
    27841 actcatggtc acttgtttga catcaatttc aactttcaaa agttggacta ctgggctcag
    27901 gaggaagagg ccgctatctg cctctatggt cacttgcatg tgccaagtgc ttggatggaa
    27961 ggcaagatcc tctttctaaa tccaggttct atcagccaac cacgaggtac catcagagaa
    28021 tgtctctatg ctcgtgtgga gattgatgat agttacttca aagtggactt tttgacacga
    28081 gatcacgagg tgtatccagg tttgtccaag gagtttagcc gatgattgcc aaggagtttg
    28141 agactttctt gttggggcag gaggaaactt ttttgacccc tgctaaaaat ctagctgtgt
    28201 tgattgatac ccacaatgcg gatcatgcga ccctcttgct cagtcagatg acctataccc
    28261 gtgttcccgt tgtgacagat gaaaaacagt ttgttgggac gattggactc agagatatta
    28321 tggcttatca gatggagcat gacttgagcc aagaaatcat ggcggatacg gatatcgttc
    28381 atatgacaaa aacggacgta gcggttgttt cgcctgattt caccattacg gaggtcttgc
    28441 acaagctagt agatgagtcc ttcttaccgg ttgtggatgc agagggtatt ttccaaggga
    28501 ttattacgcg caagtccatc ctcaaggccg ttaatgccct cttgcatgac tttagtaagg
    28561 aatatgagat tcgatgccaa tgagagacag gatttcagcc tttttagagg aaaagcaggg
    28621 cttgtctgtc aattccaagc agtcctataa gtatgatttg gagcaatttt tagacatggt
    28681 aggtgagcgg atttctgaga ccagtctcaa gatttaccaa gcccagctag ccaatctaaa
    28741 aatcagcgcc cagaagcgaa agatttcggc ctgtaaccaa tttctatact ttctctatca
    28801 aaaaggagag gtggacagct tttaccgctt ggaattagcc aaacaagctg aaaagaagac
    28861 ggaaaagcca gagattctat acctagactc ttttttgcag gaaagcgacc atccagaggg
    28921 ccgcttgcta gcgctcttaa tcctagaaat ggggctcttg cccagtgaga ttttagccat
    28981 caaggttgcg gacatcaatc tggattttca ggtgctgcga atcaacaagg cttctcaaca
    29041 gaggattgtc accattccca cggccttgct ttcagaattg gaacccttga tggggcagac
    29101 ctatcttttt gaaagaggag ggaaacccta ttctcgtcag tgggcctttc gtcagttaga
    29161 atcttttgtc aaggagaaag gttttccatc cttatcagct caagtcttac gtgaacagtt
    29221 tattctaaga caaatagaaa acaaggtcga tttgtacgaa attgcaaaaa aattaggatt
    29281 aaaaacagtc ctgaccttag aaaaatatag ataatggata ttaaattaaa agattttgaa
    29341 ggacccctgg acttactctt gcatctggtt tctaagtacc agatggatat ctacgatgtg
    29401 cccattacgg aagtcatcga acagtatcta gcctatgtct caaccctgca ggccatgcgt
    29461 ctggaagtga cgggtgagta catggtcatg gctagtcagc tcatgctgat taagagtcgt
    29521 aaactccttc cgaaggtagc agaagtgaca gacttggggg atgacctgga gcaggacctc
    29581 ctctctcaaa tcgaagaata tcgcaagttc aagctcttgg gtgagcactt ggaagccaag
    29641 caccaagaac gggcccagta ttattccaaa gtgccgacag agttgattta cgaagatgcg
    29701 gagcttgtgc atgacaagac gaccattgac ctctttttgg ctttttcaaa tatcctagcc
    29761 aagaaaaaag aggagtttgc acaaaatcac acgactatct tgcgggatga gtataagatt
    29821 gaggacatga tgattatcgt gaaagagtcc ttgattggac gagatcaatt gcgcttgcag
    29881 gatttgttca aggaagccca gaatgtccaa gaggtcatca ccctcttttt ggcaacccta
    29941 gagttaatca aaacccagga gctgatcctc gtgcaagagg agagtttcgg agatatctat
    30001 ctcatggaaa agaaggaaga aagtcaagtg cctcaaagct agacttgata gagaggaaag
    30061 atgagtactt tagcaaaaat agaagcgctc ttgtttgtag cgggtgaaga tgggattcgg
    30121 gtccgccagt tagctgaact cctctctctg ccaccgacag gcatccagca aagtttagga
    30181 aaattagccc agaagtatga aaaggaccca gattccagtt tggctttgat tgagacaagt
    30241 ggtgcttata gattggtgac caagcctcaa tttgcagaga ttttgaagga atactctaag
    30301 gcgcctatca accagagctt gtctcgggct gcccttgaga ccttgtccat tattgcctac
    30361 aaacagccga ttacgcggat agaaattgat gccatccgtg gagttaactc gagtggagcc
    30421 ttggcaaagt tgcaggcttt tgacctgata aaggaagacg ggaaaaagga agtattgggg
    30481 cgccccaacc tctatgtgac tacggattat ttcctagatt acatggggat aaaccattta
    30541 gaagaattac cagtgattga tgagcttgag attcaagccc aagaaagcca attatttggt
    30601 gaaaggatag aagaagatga gaatcaataa gtatattgcc cacgcaggtg tggccagtag
    30661 gagaaaagca gaagagctga ttaagcaagg cttggtgacg gttaacggcc aagtggtgcg
    30721 tgaactagca accactatca agtcaggcga caaggtcgaa gttgaaggtc aacctatcta
    30781 caacgaagaa aaggtctact atctgcttaa caaaccacgc ggtgtcattt ccagtgtgac
    30841 agatgataag ggtcgcaaga cggttgtcga cctcttgccc aatgtcaaag agcgtattta
    30901 ccctgtgggt cgtttggact gggatacatc aggagtcttg attttgacca atgatgggga
    30961 ctttacagac gagatgattc accctcgtaa tgagattgac aaggtttatg tcgcgcgtgt
    31021 taaaggtgtg gccaataagg acaatctccg ccccttgacc cgtggtcttg agattgatgg
    31081 taagaaaacc aagccagctg tttatgaaat tctcaaagtg gacccagtca aaaatcgctc
    31141 tgtggtgcag ttgaccatcc atgaagggcg taaccatcag gttaaaaaga tgtttgaagc
    31201 tgttggtctc caagtagata agttgtctcg gactcgtttc ggacacctag acttgacagg
    31261 actccgtcca ggagaatccc gtcgtcttaa taaaaaagaa atcagccaac tacacaccat
    31321 ggctgtaact aagaaataat gaaacgaatt ttaatagcgc ttgtgcgctt ttaccaacgt
    31381 tttatctcac cagtctttcc accctcttgt cgctttgagc tgacttgttc caactacatg
    31441 attcaggcta ttgaaaaaca tggttttaag ggggtattga tgggcttggc tcggatttta
    31501 cgttgtcatc cctggtcgaa aacaggtaag gaccccgttc cagaccgctt ttcccttaaa
    31561 cgaaatcaag aaggggagtg aggtggggta aatagatttc aaaatgataa aaacgcatcc
    31621 tatcaggttt gagtgaactt gataggatgc gttttagaat gtcaaaattt tatactcttc
    31681 gaaaatctct tcaaaccgcg tcagctttca tctgcaacct caaaacagtg ttttgagcaa
    31741 cctgcggcta gttttctagt ttgctctttg attttcattg agtattaaat tgagtttgaa
    31801 gtggcttatt tcaaagcttt ttgtatgtct tcaatcatga gttttgttga ttcaagtccg
    31861 cctccgctta gataccagag gtctggtgtt agttggataa tcttaccatt tttagcagca
    31921 ggtgtttcag cgataagggc attttctagg acaccgtcgt tgctagagtt gtccccaccg
    31981 atggcaaggg tacggttgat gacaaagagg atgtcagggt tgatttcttt gacactttca
    32041 aagctgactt cttgtccgtg gcgtgagtct tcaaattttg tatcagttgg tttgaatttc
    32101 aaggtttggt acaagaaaga gaaacgagat ttggcaccaa aggctgccat ttttccttca
    32161 ttaaggagga tcgcaagggc ttttttgtca gagctttcat ttttagtagc gacttcttgg
    32221 atgctcttgt ctagcttggc caattcttcc ttggctttct gtgtaccagt ttcgccgaag
    32281 gcacttgcta aggattcgat attagccttg gtagaagtcc agtagtcgtc cttgcttgct
    32341 tggaagagaa cggttggggc gatttctttg aatttgtcta cgaatttttg tgtacgtggt
    32401 gaagcgataa tcaaatcagg ctcaagggcg gcgatagctt ctaaatcagg ttctttcata
    32461 gaaccaacat ttttgacagt tcccactagg tcttttagat aagtcggaac agtttttgta
    32521 ggcattccga cgatattttt ttcaaatcct aaagcgcgaa tagtatccgc agcgccgagg
    32581 tcaaaggtca caatcttttt aggaactttg gaaagtttga cctcgtccag tgaactttta
    32641 atggttacct ctgttggagc agagctactg gtctctgtct gactagtgct tgagtttgta
    32701 ctacatgcac caagtaggag caagaagctg gccactaggg cagtgaaata aagtttaagg
    32761 gatgttttca taatttctcc tttttaaaat gtgataacga tttagggagt ctcttaatct
    32821 tattgactaa gagactgaag gttctctaac ttgagctttt atgttactag ctatagatac
    32881 agatcttttt gtcattgata tcagctagcg tgatgggaat ctcataaagt tgactgagca
    32941 ggtcagcctg catgatttga tcggttcttc ccttgctaaa gacctggccg tccttgaagg
    33001 cgacaatttc atctgcatac tgactggcca tgttgatatc gtggaggacg atgataatgg
    33061 tcttgccgag ttcctccacc agtcgtcgaa gaatctgcat catgctgacg ctttgcttga
    33121 tatcgagatt gttgagtggt tcgtccagca agataaagtc cgtatcctgg gccagtacca
    33181 tagcgataaa gacgcgctgg agttgccccc ctgacaggct attgatgtag cggtctttta
    33241 agttggtcag ttctaaatag ttcagagttt ctcggatttt ttcccagtct tctgatctaa
    33301 gtcgacctcg gctgtaggga aaacgtccaa aactgaccag ttcttcaaca gtcaatttgg
    33361 cttggtaatt gattttctgt tttaggatgg ttagttctcg ggccagttct tgcgaattcc
    33421 agctctcgat ttcacgtcct ttgatactga gaactccctg atctttcttg gttagcctgc
    33481 tcatgatgga gaggagagtc gattttccag caccatttgg accaataaag gctgtcagtt
    33541 tttgaggact gacttcaagc gaaatgcctt gcaaaatatc ctgtttttga atggatttgt
    33601 caatgttttc cagtttcact gacgagacct cctatatagt aagataaaga ataagaagcc
    33661 acccacactc tcaatgatca tactgatacg aatttccagt gcaaagactc gttcaatcaa
    33721 ggcttgcccc aaggttaagc taataaatcc aaccagaatg gccactataa agagtaactt
    33781 gtgctgatag tctttgacaa tcaggtaggt gaggttggcc agtataaagc cgaagaaggc
    33841 cataggtcct accaaggcag tggccgttga ggtcaaaagc acgattcccc agaggagctc
    33901 tttctgttct ttttcaacat cgagtcccaa tatctgagcc gtttctcttt gcaggtgcaa
    33961 gacatctaga acgactgctt ttcgaaagaa aaagattgtc aaagcgagga tgatcagaga
    34021 accgatggct aggatggaag tgttgagatg ttgaaaggag gcaaaaagac tattttgcag
    34081 tttatcgtat tcgtttggat ccattaggac ttgaaggaag gtgctgatat ttcgaaagag
    34141 acttctgagc gctagacaga tcagcaggat gaagaccagg tcttgcttca tcagtgtctt
    34201 caagtaacct tgtaaggcga gaaagaagag ggactggaca agaagtaaga ctaggaattc
    34261 taagataggg gatttgccaa gttgaagaaa cttgctttca aaaaccagta gtagggtttg
    34321 tagtaggacg tagaaggatt caattcccaa aatactaggc gtcaggaagc gattttccgt
    34381 cagggtttga aaactaatgg tcgaaatccc agtcgcgatg gctaccaaga gataaacgat
    34441 gatcttttgg gaacgcaact tccaagcaaa ggctgacaag tgagtgatgg gccaaaagta
    34501 gagaagacaa gctccgatgg caagaataat gagaatccag aagagcttgg tatgtttgct
    34561 tttagtctgc atcttttcgt ccccctctcc agagaagtag gataaagacg agactaccga
    34621 tgattcctag caagagactg acagacaact catagggcct aatcagaact cgggatagga
    34681 tatcgcaagc cagaactaga ttggcaccaa ccagtgcgac catgagtttg gtttgactta
    34741 gattatctcc atagcacttg cgaacaagat tgggaacgat aactccgaga aatggtaggc
    34801 cacccacggt aatcatggtg acgcttgtcg ttagcgccac cagaaagagg gccagttttt
    34861 caagtaggga gtaggaaatc cccaaactct cgctggtttc tttccctaga ttcatgatgg
    34921 tgaaggtttg ggataatttc caaacggtta tcaggatgat gaggcctaag aagagccact
    34981 catactgatg ggtctgaatc atggagaagg agccctgggt ccaggcagtc atactctgaa
    35041 ccagattgaa acgataggcg ataacttctg tgactgagcc gataatcccg ctatagatga
    35101 tcccaatcag aggcaacatc cacctttcct ttacagtaaa aatggtcata aaggctagga
    35161 agaagagggt gaatacgatg gatgaaacaa aagcgaagag catcttttgg gtcagactag
    35221 ccgatggaaa gacaaaaagg ctcagcacca ttcccagttt ggcggcttca gtcgttccaa
    35281 ctgtactcgg tgcagcaaac tgattttggg taatagtctg catgagaagg cctgccatac
    35341 tcatactaga ggcagtcagg agaatactga tagttcttgg gagacgggac tcttgaaaga
    35401 ggagccaggt ctgctggtcg aaatcaaata gctttcccca tgaaaaatca ctggtcccaa
    35461 tgctaataga gagaaagact aggagtagaa gtaagccaat taaataatga gaaagtttca
    35521 taccccgtcc tttcatgtag atttggtatc gaaagatatc tgcggatata aatgtaacat
    35581 tatttttcta aatctgtcaa taaaatttct gacaatttaa taaatacaac aaggagagag
    35641 caacaagact ttctcctttg ttatcctatt ctaaaatgtt ttaccttgat ctgataaaat
    35701 aatatcttcg agggagtagc tagccgtcca atcaagatat tgtttagctt ttgaagcatc
    35761 tgctaggaca ctggctgggt cactagcacg tcgagcaaca atctcgtgtg ggatttttta
    35821 atttagtaat tcttcagcag ttttaaagat ttctttgata gtatagcctt ttttagttcc
    35881 taagttaaag atttgagaag aactgtcttc ttgaaatagg tagttcattc ctttaacatg
    35941 agcctatgaa ggtccaagac ataaatgtaa tctcgaatac atgaaccgtc atgtgtatcg
    36001 tagtcatctc caaatatttt taagctatca ttttgtccca atgcggtctt gttgatattt
    36061 ggaatgatgt gagttggatt tttcacacgc agaccgtttg aagcatccat ttcagcccca
    36121 gcaacattaa agtaacggaa aataacatat ttccagtcgt agcgattggc catccagtaa
    36181 atcattcgtt cgcccatcag ttttgtctct gcataagggt tgacagggtc gagcagggta
    36241 tcttcagtca ccggcttgtc aatacagtta tttccataga gagaagcagt cgaagagaac
    36301 atgatttttt gaatgccaac ttcagataag actttgagaa cttggttcct accagcaacg
    36361 ttggcagtga agtatttact tggattttca atactttcgc tcacaacaat ttcacctgca
    36421 caatggagga cagcatcaat ctgattttct tctaagtaag cctttaaggc gctagcatca
    36481 taaacatcca gttctttaaa gctagcacga ctatccacag ccgctcgatt tcctgtggag
    36541 agattatcga ggacatgcac cttatagcca gcatttaaaa gagctttaac ggtatgggag
    36601 ccaatgtagc tagtcccacc tgtaacaaga attgttttgg tcatgatttt ttccttttct
    36661 agttttgtac tatagtaaat tgaaataaga tataaacaac tctattagga aagtcaaatt
    36721 aatttctaga aatgttttag cagctacagc gtactattcc aaactcaacc aactatatat
    36781 ttaaagaaag aagaaaggga agtcaactat tttctgtaaa tttcataaaa aatctaaaca
    36841 actttgatgg tgtattgttt ttttgcttgg aaataaatat tcgagttaat tttggaatgt
    36901 ttactagatt ttgcactaca tgcaagagaa tactatcctt ttctaagata gagcaggact
    36961 aagatactaa cttagaaatc caccttccca ctatccttct cctataaaaa gtggtaaaat
    37021 ggatgaaaga aagaaggaac tgaaatgaca acattatttt caaaaattaa agaagtaaca
    37081 gaacttgctg cagtctcagg tcatgaagcg cctgtccgtg cttatcttcg tgaaaagttg
    37141 acaccgcatg tggatgaagt ggtgacagat ggcttgggtg gtatttttgg tatcaaacat
    37201 tcagaagctg tggatgcacc gcgcgtcttg gtcgcttctc atatggacga agttggtttt
    37261 atggtcagcg aaatcaagcc agatggtacc ttccgtgtcg tagaaatcgg tggctggaac
    37321 cccatggtgg ttagcagcca acgtttcaaa ctcttgactc gtgatggtca tgaaattcct
    37381 gtgatttcag gttctgttcc tccgcatttg actcgtggaa aggggggacc aaccatgcca
    37441 gccattgccg atatcgtttt tgatggtggt tttgcggaca aggctgaggc agaaagtttt
    37501 ggcatccgtc ctggtgatac catcgtacca gatagttctg caattttgac agccaatgaa
    37561 aaaaatatca tctcaaaagc ttgggataac cgctacggtg tcctcatggt aagcgagcta
    37621 gctgaagctt tatcaggtca aaaactcggc aatgaactct atctgggttc taacgtccaa
    37681 gaagaagttg gtctgcgtgg cgctcatacc tctacaacca agtttgaccc agaagtcttc
    37741 ctcgcagttg attgctcacc agcaggtgat gtctacggtg gtcaaggcaa gattggagat
    37801 ggaaccttga ttcgtttcta tgatccaggt cacttgcttc ttccagggat gaaggatttc
    37861 cttttgacaa cggctgaaga agctggtatc aagtaccaat actactgtgg taaaggcgga
    37921 acagatgcag gtgcagctca tctgaaaaat ggtggtgtcc catcaacaac tatcggtgtc
    37981 tgcgctcgtt atatccattc tcaccaaacc ctctatgcaa tggatgactt cctagaagcg
    38041 caagctttct tacaagcctt ggtgaagaaa ttggatcgtt caacggttga tttgattaaa
    38101 cattattaaa ccataaggga ggtggtaggg atggtagaac caaacctaga aagccttata
    38161 aaagatcttt acaatcatgc tcgacatgat ttgagtgaag atttagttgc tgctctccta
    38221 gagactacta aaaaattgcc tactacaaat gagcaattgc aggcagttcg tctctcaggc
    38281 ctggtcaatc gtgaattgct cctaaatccc aaacatccag cacctgagtt gctcaacttg
    38341 gctcgcttta tcaaaagaga agaagccaag tacagaggaa ctgcgacttc tgcgcttatg
    38401 tatgaggaac tttttaaaat gctttgattc cattgtggat caaagcattt tttataagac
    38461 ctatctcgtg agcgttcaaa tagtagtaac aaggaatcat gagaagtcag ccgagaccat
    38521 agtagtgaag aaacttctgt aatggaagtg gagtgaagtg aggaatcgtc aaacggtagg
    38581 ggataggctg tttgaagggt gctacgaaaa agatagggta aggtgaaatt atagcggaat
    38641 gaaataagat atgaacaaat tgattaggga aatcaaatga atttatagaa atcttttagc
    38701 agtcatggtg tcctattcca gtttcgaaat gctataaata cgaacaaatt gattgggaaa
    38761 ttcaagtcgc tttttatcaa tgttttagaa gatacagtgt cctagttcaa cttcaatata
    38821 ctataaaaga acagatggaa gcaattatga tttacttcta tctttaaaat aaatggtctg
    38881 ataggaaaaa tgtgtcccct cataaaatat aggatagtac ggatgaaagg cttttgtaga
    38941 ctggaggacg aaggagttta gaaactctcc ttaaaagtta gttgatccgg agtatggtga
    39001 tgtaaataat tatcaaaaat tatttctaaa tcgaaatagt tgacatatgg aatgatgagt
    39061 gttataatca ctttatctat ttcgatatag aaataaattg gaggtttcat gaaatattgt
    39121 catttggtag cccatcatat tcgtttgttg aatgggcgga tttttcaaaa gttactaaga
    39181 caagaccctg actctcttta tcggagtgag cagggaaaga ttttagcggt tttatggaat
    39241 agtgagacag gctgtgctac ggcgacagac attgcgcttg cgactgggct tgctaataat
    39301 acgctgacga ctatgataaa aaatctagag gaacaaaatc ttgtaactgt cagtccgtgt
    39361 ggagaagata agcgtaagaa gtatttagtt ttaacagagt tgggcaagtc ccagaaagaa
    39421 gttgggcaac gtgtcagtca gagattggat actatctgtt acaaaggatt ttcagaggaa
    39481 gaaattcgtc agtttgaagg ttttcaagaa agaattttgg caaatctgaa agagaaggaa
    39541 aatgaggttt agaatatgtc acaacaagtg aaaaatgctc acaacctata catccatgcc
    39601 attcaagacg gacgagtttc tgaggctcaa gcccagtctg taggggatac ctatatccaa
    39661 cattcgacag gtgttcctga cgggaaagaa ggatttgcag ccttctttgc agatttcttt
    39721 gagcgtcatc cagagcgtca gattaagatt gtccgcacca ttgaggatgg caatctggtc
    39781 tttgttcatg ttcatcaata tctgaatggt ggagaagctc aatgggtgac gacggatact
    39841 ttccgtgcgg atgagaatgg tcgtattgtt gagcattggg atgtcattga ctactatcga
    39901 acacctgaaa atgaccaact agatcaaatt tttggagatt ttgaaatcaa ggatttggac
    39961 aagaaagcag aaaataaaaa gctggttcgc cgtttcttga cagaaatttt ccaaaatggg
    40021 gagctagagc agtggagtga ttatgtggca gacgatttga ttcagcataa tcatgagatt
    40081 ggacaaggaa gtgctgctta taaaaactat gtggctgaat atagtgtcac ttttgacttt
    40141 gttttccaac tcttaggaca aggaaactat gtggttagct atggtcagac tcagattgat
    40201 ggcgttgctt atgcccagta cgatatcttc cgtttagaga acgggaaaat tgtggagcat
    40261 tgggataata aggaagtcat gcctaaggta gaagacttga ctaatcgagg gaagttttaa
    40321 attgaggaca aagaatgatt gaatacaaaa atgtagcact gcgctacaca gaaaaagatg
    40381 tcttgagaga tgtcaattta cagattgaga atggggagtt catggtttta gttgggcctt
    40441 ctgggtcagg taagacgacc atgattaaga tgattaaccg tctcttggaa ccaactgatg
    40501 gaaatattta tatggatggc aagcgcatca aagactatga tgagcgtgaa cttcgtctta
    40561 ctactggata tgttttacag gctattgctc tttttccaaa tctaacagtt gcggaaaata
    40621 ttgctctcat tcctgaaatg aaggggtgga gcaaggaaga aattacgaag aaaacagaag
    40681 agcttttggc taaggttggt ttaccagtag ccgagtatgg gcatcgctta cctagtgaat
    40741 tatctggtgg agaacagcaa cgggtcggta ttgtccgagc tatgattggt cagcccaaga
    40801 ttctcctcat ggatgaaccc ttttcagcct tggatgctat ttctcgcaag cagttgcagg
    40861 ttctgacaaa agaattacat aaagagtttg ggatgacaac gatttttgtg actcatgata
    40921 cggatgaagc cttaaagttg gcagatcgta ttgctgtttt gcaggatgga gagattcgtc
    40981 aggtgtcgaa tcctgagact attttaaaag ctcctgcaac agactttgtt gcagacttgt
    41041 ttggaggtag tgttcatgac taatttaatt gcaacttttc aggatcgttt tggtgattgg
    41101 ttgacagccc tatctcaaca tttgcagttg tcacttttga ccttgttact agctattttg
    41161 cttgcgattc ccttggctgt ttatcttcgc tatcatgaga agttggcgga ttgggtctta
    41221 cagattgcag ggattttcca gaccatcccg tctctggcct tgttgggact ctttattccc
    41281 ttgatgggaa ttgggacttt accggcattg acagctctag tgatttatgc gatttttccg
    41341 attttacaaa atactattac tgggctgaag ggaattgatc cgagtcttca agaggctggg
    41401 attgcctttg ggatgaccag atgggagcgt ctcaagaagt ttgaaattcc acttgccatg
    41461 cctgttatca tgtctggaat tcggacggca gcggtcttga ttatcggtac ggctaccttg
    41521 gcgaccttga ttggggcagg gggactgggt tcctttatcc tgttgggaat tgaccgtaat
    41581 aatgccagtt tgattttgat tggggccctt tcttctgcag tgctagctat tgcctttaac
    41641 ttcctactaa aagtaatgga aaaagcgaaa ttgcggacga ttttctctgg ttttgccttg
    41701 gtggctttat tactgggtct gtcttatagt ccagcccttt tggctcaaaa agagaaggaa
    41761 aacttgatta ttgctgggaa aataggccca gaaccagaaa ttttggctaa tatgtataaa
    41821 ttgctgattg aagaaaatac cagcatgact gcgactgtta aaccgaattt tgggacgaca
    41881 agtttccttt atgaagctct gaaaaaaggt gatattgaca tttatcctga atttaccggt
    41941 acggtgactg aaagtttgct tcaaccatca cccaaggtga gtcatgagcc agagcaggtt
    42001 tatcaagtgg cgcgtgatgg cattgccaag caggatcatc tagcctatct caaacctatg
    42061 tcttatcaaa atacctatgc tgtagctgtt ccgaaaaaga ttgctcaaga atatggcttg
    42121 aagaccattt cggatttgaa aaaagtggaa gggcagctga aggcaggctt tacgcttgag
    42181 tttaacgacc gtgaagatgg aaataagggc ttgcaatcaa tgtatggtct caatctcaat
    42241 gtggcgacta tgcagccagc tcttcgttat caggcaattc agtcagggga tattcaaatc
    42301 acggatgcct attcgactga tgcggaattg gagcgttatg atttacaggt cttggaagat
    42361 gacaagcaac tcttcccacc ttatcaaggg gctccactca tgaaagaagc tcttctcaag
    42421 aaacacccag agttggaaag agttcttaat acattggctg gtaagattac agaaagccag
    42481 atgagccagc tcaactacca agtcggtgtt gaaggcaagt cagcaaagca agtagccaag
    42541 gagtttctcc aagaacaagg tttgttgaag aaatgatggg acagagactt taaacatact
    42601 gaactcaact tccttaaacg actagataaa agaatgctca gacaatttcg tctgggcttt
    42661 tttgatgtga gaatgactga atgtcactta aaatggaaag aaagagaaaa ttttcatgat
    42721 tttctaaaat tttactgtaa agacagttga ctattcataa aataggtgta taatattttg
    42781 tgaactactt aacaaataag aggtttcagc tcatgtcgac taaggttaga aatcttgtgt
    42841 ataggcagtt cattatctaa tagagcgttg cgtccgaaag tctatccaga cacggctctt
    42901 taaaaacaaa aggagaaatg atgcatactt atttgcaaaa gaaaattgaa aatatcaaaa
    42961 caaccctagg tgaaatgtca ggtggttacc gtcgtatggt tgcggctatg gctgatttag
    43021 gattttcagg aactatgaag gctatctggg atgacctctt tgcccatcgt agttttgccc
    43081 agtggattta tttgctggtt ttaggaagtt ttcctctctg gctggagttg gtttacgaac
    43141 atcgtattgt tgactggatt gggatgattt gtagcttgac agggattatc tgtgtaatct
    43201 ttgtatcgga aggtcgagca agtaattatc tttttggctt gattaactct gttatttacc
    43261 ttattttggc cctacagaaa ggcttttatg gtgaggtgct gacgacactt tacttcacag
    43321 tcatgcagcc aattggactt ctagtttgga tttatcaggc acagtttaag aaggaaaagc
    43381 aggagtttgt cgcgcgtaaa ctggacggca agggctggac aaagtatctt tccattagtg
    43441 tgctttggtg gttggccttt ggcttcattt atcagtctat tggtgccaat cgtccctatc
    43501 gtgattcaat cacagatgca accaatgggg tagggcaaat cctcatgaca gctgtttacc
    43561 gtgaacagtg gatattctgg gcggctacca atgttttttc aatctatctc tggtggggag
    43621 aaagcctgca aattcaaggg aaatatctaa tttatctcat taacagtcta gttggttggt
    43681 atcaatggag caaggcagct aagcagaata ctgatttact taactaggaa aagatgtttg
    43741 aaagtgctgt tttgagattt cgattaaaac agatatagtt gataatcaag gatttatagt
    43801 atgaaaaaga ggatcggcgg gtcctctttt gttgttgaaa agataaaaaa ctcagtaacc
    43861 tagaaataag acaactgagc tttactctat attcaatttt taggaatgag aaggtctaga
    43921 taaaattgga caacttcctg gtctgtgaaa tcttgacctt ttttgagcca ccaggtcaat
    43981 gtctcgataa agttggacac gaccaagtgt tggaggtaag aagtaggcat attagggtgg
    44041 gcttctttta aattatcagc tagcacggaa tagacatggt gttctagctc tttatggagt
    44101 tgacggagga agtagtcatt tttggaaaat agcagactgg tgatatggtc ttggttttta
    44161 tgaaaatgga gaaagaggtg ggcgaggtag tcctcggttg aaatggcttg ctctctttca
    44221 aaaagatgat ggaagaggta gcggcagagc tggtccagaa gaagctcctt actttcatag
    44281 tgacagtaaa aggtggatct tcccacatct gcgagatcaa tgatatcctg aacagtagtg
    44341 gcctcgtagc ccttagcatt caaaagttgt ataaaagctt gatagatggc ttttttggtt
    44401 ttgctgatac ggcggtcaat gttagtcata tggacactta aggcaaattg ttcagaactg
    44461 aataaagctg acgttttgct tctatccttt ctttgagttt tagtggataa tgataatgaa
    44521 caaggtgttc ataaacctat tataacaaag gaatgagaaa tatgaaggca aaatatgctg
    44581 tttgggtggc ttttttctta aatttgactt atgccattgt tgagtttatt gcaggtggag
    44641 tatttggttc tagcgctgtt cttgctgact ctgtgcatga cttgggagat gtgattgcaa
    44701 ttggaatatc agcttttcta gaaacaatct ccaatcgtga agaagacaat cagtacacct
    44761 tgggctataa gcggtttagc ctactaggag ccttggtaac agctgtgatt ctcgtaacag
    44821 gctctgttct agtcattttg gaaaatgtca cgaagatttt gcatccgcaa ccagtcaatg
    44881 atgaggggat tctctggtta ggaattattg cgattactat caatctgtta gcgagtctgg
    44941 tggttggtaa gggaaagaca aagaatgagt ctattctgag tctgcatttt ctggaagata
    45001 cgctagggtg ggtagctgtt atcctgatgg cgattgttct tcgatttacg gactggtata
    45061 tcctagatcc tcttttgtcc cttgtcattt ctttctttat tctttcaaaa gccctttcac
    45121 gtttttggtc tacactcaag attttcttgg atgctgtgcc agaaggtctt gatatcaagc
    45181 aagtaaagag tggcctggaa cgattggaca atgtggccag ccttaatcag cttaatctct
    45241 ggactatgga tgctttggaa aaaaatgcca ttgtccatgt ttgtctaaaa gaaatggaac
    45301 atatggaaac ttgtaaagag tctattcgaa ttttcctaaa agattgtggt tttcaaaata
    45361 ttaccattga aattgatgct gacctagaaa ctcatcaaac ccataagcga aaggtgtgtg
    45421 acttggaacg gagttatgag catcaacatt agaaaaaagt gaaaaatact tgggtactat
    45481 cttatttgga atagagtaat ttctttatta tttaaatatt tcaaaaattg gtaagagaag
    45541 agcattgtat aaactccaga tatatgattg ttaatgataa aaatttttcg attagataca
    45601 aaatgcttga cttggagtca actcaaagtt atataataag ataagtgagt tagaatagcg
    45661 tgaactcagt gaatgaaatg agaggaggtt agcgtgtgaa tattaaatct gccagtgatt
    45721 tgttgggaat ttcagcggat acgattcggt attatgaacg ggttggtctt gtgccaccga
    45781 ttactcgtac tgctactggg attcgtgatt ttcaagatca ggatatcgaa gcgctggaat
    45841 ttattaagtg ttttcgttcg gcgggtgtct ctgtagatag tttagttgac tatatgtcgc
    45901 tctaccaaaa gggagatgaa acgagagagg agaggcttgg tattttagaa gaggaaaagc
    45961 aaaaattaga ggagcgcttg tctcagctac agacagcttt aaatcgttta aatctcaaaa
    46021 ttaaacttta taaggaagga aaattttaaa tgaaatcagc agtatataca aaggcaggtc
    46081 aggttggact tgctagcatt gaacgtccgc aaataataga agcggatgat gtgattattc
    46141 gtgtggttcg tgcgtgcgtt tgtggttcag atttatggag gtaccgtaat ccagaaacga
    46201 aagctggaca caaaaatagt ggacacgaag cgattgggat tgttgaagaa gctggggaag
    46261 ccattacgac ggtgaaacca ggtgattttg tgattgtccc ttttacacat ggatgtggtg
    46321 agtgtgatgc ctgtcttgct ggatttgacg gttcttgcga caatcatatt ggcaataatt
    46381 tggggggtga ttttcaggca gaatatattc gcttccacta tgcaaactgg gcgctggtta
    46441 aaatccctgg tcaaccttct gactatacag aagggatgct caagtccctt ttgactcttg
    46501 cagatgtcat gccgacaggc tatcatgcgg cgcgtgttgc aaatgttcaa aaaggggaca
    46561 aggttgttgt tatcggtgat ggggctgttg gtcaatgtgc tgtcatcgcg gctaagatgc
    46621 gtggagcatc acaaattatc cttatgagcc gtcatgaaga ccgtcaaaag atggctatgg
    46681 agtcaggtgc gacagctgtt gttgcagaac gtggtcaaga aggaattacc aaggtgcgtg
    46741 aaatcctcgg tggaggagca gatgcagcac ttgaatgtgt tggtacggag gctgctatag
    46801 aacaggcgct aggtgttctt cataatggag ggcgtatggg ctttgtagga gtcccacact
    46861 ataataatcg tgctcttggt tcgacattta tgcaaaatat ctctgtagca ggtggggcag
    46921 cttctgctac aacatacgat aagcaatttt tactaaaagc cgtccttgat ggtgatatca
    46981 atccaggtcg cgtctttact tcaagttata aactggaaga tatcgaccaa gcctataaag
    47041 atatggatga acgtaagaca attaagtcta tgattgtaat cgaataaaaa acgaatagga
    47101 gttttagaac tctattcgtt ttttatgtta tcctattctt gatttagggt actttctctt
    47161 aatgtcagtc tggttcccag catggtcagg ctagggattt tccgaccgtg gaggacttcc
    47221 ttgttaagaa tatccatacc tgctcgcccc atttcttcag tataaactgt gatgctggag
    47281 aggggaggat aaacctgctt ggtcaggctg gtgtcgttaa aggaaatgag gctgacacgg
    47341 tctggtaggc tgattccagc ttcttggagc gcacgaaggg caccgatggc taaactatcg
    47401 ctggctgcga aaaatgctgg tgggagttgg tctcccaagt tctgaatggc ttccttcatc
    47461 aagtcatagc cagactgggc agtaaagttt ccttgaaaga tcagttcatc atgatagatt
    47521 ccttttgctt gactgtagtt tctgaaattt tccagccgct tatcctcaat gatttcttct
    47581 tggtcagttg tttcttcaag gcctgttaga atcccgatac ggtccattcc ttgactgagg
    47641 aaataatcga caacctgttt catagcagtg taaaaatccg tgataataca ggtatgtccc
    47701 agggaaagtg tatcgctgtc tagaaataca agaggctttt ggtattcttc aaaggcagaa
    47761 atctgagctc gactaaactt tccgatgcag agaatcccaa tcacttcctc gcttagggta
    47821 aaagggtggt cattaaaata gcgcaagata tcgtagtcca actcttgggc tcttttttct
    47881 attcctaggc gaatctggta gtagtagagg tcgtctagct ccccttgttc gctgacccat
    47941 tggataatgg caatcttttg cttgggttta tgggactcgc ctgtcttgag gtgcttggtg
    48001 tagcccagct cttcagcaac ggttaaaata cggtgtctgg tttcttctgt aacagatagg
    48061 ctctggtcgc ggttgaggac acgggatacg gtcgcgatag agacagaggc tagctgtgca
    48121 atgtctttta aggtagccat aaatcctcct tgattaggtt agtatatcat atttttcttc
    48181 tttttactga tagtttagta aaattttagt aaaaaggatt gaccttggga aatcccttgg
    48241 atacaataga agaaaacgat tacacgttaa ggtggcttaa cggacagtca aaggagaaat
    48301 catatgacac aacatcttac tgctgaaact cttcgcaaag actttcttgc tgtttttggt
    48361 caagaagcag accaaacttt cttttcacca ggtcgtatca atttgattgg tgaacacaca
    48421 gactacaacg gtgggcacgt ttttcctgct gctatttcct tgggaactta cggtgcagct
    48481 cgtaagcgtg acgaccaagt tttgcgtttc tattcagcta actttgagga caagggcatt
    48541 atcgaagtgc ctctcgctga cctcaagttt gaaaaagagc acaactggac caactatcca
    48601 aaaggggttc ttcatttctt gcaagaagct gggcacgtga ttgacaaagg ttttgatttt
    48661 tatgtttatg ggaatatccc aaatggtgct ggtttgtctt cttcagcatc cttggagctc
    48721 ttgacagggg tagtggcaga gcatctcttt gatttaaaat tagagcgtct cgatttggtt
    48781 aaaatcggaa aacaaacaga aaataacttt atcggagtca actctggtat catggaccag
    48841 tttgctattg gtatgggggc tgatcaacgt gctatttacc ttgataccaa caccttagag
    48901 tatgatttgg tgccacttga tttgaaggac aatgtcgttg ttatcatgaa caccaacaaa
    48961 cgccgtgaat tggcggactc taaatacaat gaacgtcgtg ctgagtgtga aaaagcagtg
    49021 gaagaattgc aagttgcctt ggatattcag accctgggtg aattggacga gtgggccttt
    49081 gaccaatata gctatctgat taaagatgaa aatcgtttga aacgtgctcg ccatgctgtg
    49141 cttgaaaacc aacgtaccct taaagctcaa gcagcccttc aagcaggtga tttggaaaca
    49201 tttggtcgtt tgatgaatgc gtcacacgtt tctctggagc gtgattatga agtaactggt
    49261 ttggaattgg atactcttgt tcacacagct tgggcacaag aagaagttct cggtgctcgt
    49321 atgacagggg ctggttttgg tggctgtgcc attgccttgg ttcaaaaaga tactgttgag
    49381 gcctttaagg aagctgtagg caaacactac gaggaagtag ttggatacgc tccaagcttc
    49441 tatatcgctg aagttgcagg tggcactcgc gtccttgact agtcaaaagg aggctctata
    49501 gtgatcttag tagataaatt tgtaacacat gtcatttctg aaagttcatt tgaggaaatg
    49561 gatcgaatct acctgaccaa tcgtgttttg gcacgagtgg gagaaggtgt tttggaagtt
    49621 gagaccaatc tggataaatt gattgacctc aaggaccagc tagttgagga agccgttcga
    49681 ttagagacga ttgaggatag tcagactgcg cgtgaaatcc ttggtactga actgatggac
    49741 ttggtgactc cttgtccgag tcaggtcaat cgtgacttct gggaagccta tgcctactct
    49801 cctgagcaag cgatagagga tttttatcaa ctcagtcgga aaaatgacta catcaaactc
    49861 aaggccattg ctaaaaatat cgcttatcgt gttccatctg attatggaga acttgaaatt
    49921 accatcaatc tctctaagcc tgaaaaggat cccaaagaga ttgcggtagc caagttggtg
    49981 caagctagta attatcctca gtgtcaactt tgtttagaga atgagggtta ccatggtcgg
    50041 gtcaaccacc cagctcgcag caatcaccgg attatccgtt ttgaaatggt tggtcaggaa
    50101 tgggggttcc agtattcgcc ctatgcttac tttaatgagc attgtatctt tttagatggc
    50161 cagcatcgtc ccatggccat tagtcgtcag agttttgaac gtctgttggc tatcgtagag
    50221 cagtttccag gatattttgc tggatctaat gccgacctgc cgattgtggg gggctctatt
    50281 ctaactcatg atcattatca gggaggccgt cacgtatttc ctatggaatt ggctcccttg
    50341 caaaagacct tccgatttgc tggttttgag caggtcaagg ctggaattat caagtggcca
    50401 atgtctgtcc tacgcttgac ttcggattcc aaagaggatt tgattaacct agctgataag
    50461 attttccagg aatggcgtca gtattcagat tctagtgtgc agattttagc agagacagac
    50521 gggacaccgc atcacaccat cacaccaata gcgcgtaagc gtgatggaca gtttgagttg
    50581 gacttggtct tgcgagacaa tcagacttca gcagagtatc ctgatggtat ctatcatccc
    50641 cacaaggatg tccaacatat caagaaggaa aatatcggct tgattgaggt catgggcttg
    50701 gcaatcttgc caccacgtct aaaagaagaa gtggagcaag tcgctagcta tcttgtagga
    50761 gaagctgtta cagttgccga ttatcatcag gagtgggcag accaactcaa atcccaacat
    50821 ccagacctaa cggataaaga aaaagccctt gcaatcgtca aggactctgt gggtgctatc
    50881 tttgcgcgtg tacttgagga tgcaggagtc tacaagcaga cagaacaagg gcagacagcc
    50941 tttatgcgct ttgtggaaca tgtcggaatt ttactagact aggagctttc tccttgcaca
    51001 gtcctataaa aagtagtatc atagtgtcgt ttgaaatatc aggaggaaac gatgaaagac
    51061 tttcattttg acgctatatc tgcctttgaa aattacgaaa ttgaaaaaat gagagatggt
    51121 cacgttgtgg tgacgaccaa agtagtgaac tcgtcgctca actactatgg caatgcccat
    51181 ggtggctatc tattcacgct atgtgaccaa atcagtggtt tggtggttat ctcgctggga
    51241 cttgatgggg tgacactcca atcctctatc aactacctta aggcaggaaa actcgacgat
    51301 gtattgacca ttaaaggaga atgtgtccat caaggtcgta caacctgtgt gatggatgtg
    51361 gatatcacca atcaagaggg cagaaatgtc tgtaaggcaa cctttactat gtttgtcaca
    51421 ggccagcggt cagaagaaag acaggtaagg atatagccaa acaaaaaaga ggctcacacc
    51481 tctttttctt attatgtgac tttcagtccg tttcgctctg ttaggtgcga gacaaaaaaa
    51541 ccacccgcta gacgggtggt catgattgtg ttcttccgat gaaatggtgg tctgacaatg
    51601 tcagccaccg tgtctagcac gggaaattta gaattgacct aaaaatatga tttcaggtct
    51661 ttgcgatctc agataatgta tacacactat cgtaaaaata gaactatcag taaaggtcag
    51721 aatgatactt ttggactgat ggttctattt tattttgggg agaatcccca agtctctatc
    51781 tatattgcta tttatttatt gctatcatga cttgttcagc aattgcttta gctagcaacg
    51841 gtgggacagc gttacctacc tgagtaaatt gaggtgtttc ttttgctcgc ttatcgcccc
    51901 cagtagtacg ttttcctaga aatataaaac tatcgtcaaa tgattgtaga cgagccattt
    51961 ctcttacagt caacacacgg ttaaaaaatg ggtggatata gtcatctggt aatgttacca
    52021 tggttggaga aacggtatct tggttaagac gaactcgaat actcttttta gtaaagattg
    52081 cttcaattgc tgcaggagtt aattctccat ttctcaacat gtttgaaaat tgattttcag
    52141 tgatttctgt attcaaccga tttttgattt cattccaaaa gagttcagca tcttcaaatg
    52201 atgtttttaa tctaagagca agtgatttta aaatatggtg ttttcttaag ttgttattat
    52261 cattcataga caataaacct agttggcgat ttgtaaattg tagccatttt tcagattgaa
    52321 aattttcttg aagcaaatct tgctttttta gttgctttag aatgataggt tggtttgttt
    52381 taccattaag ttgaaataaa ctttctgtga atagatttgg gcaatctctt tgtaattcca
    52441 atccttccaa ttggattcta ttagcagctt tggctctgtt ctcaccttgc tgatatagtg
    52501 aaaatcgttg tgtgacaata tcatcatgtc ttgagaattc catatttaac ggattagcat
    52561 ttataatagg ttgtccatca gtgttaggag ttcttcctaa tcggctttgt acggaataat
    52621 ctgtttcgta atcaaattcc ccgtcataaa gatcacctaa agcatcataa acagtaacgc
    52681 ttggaatatc gttgtcactt tctggatatt cgataggagt tacatcgtta cggtatgcta
    52741 aaaatacaac tcgatttctt tgttgtggaa caccgaagtt tgcagcgttt agaacctgaa
    52801 catcaagtag agtatatcct aactcgtcta actcatttct aagaatggat tgaactaaat
    52861 tctgtccttt gtaaattcta tcatcaagta cgcttggaaa gtcaagcatc tgcatatcta
    52921 aaatacctgt tacgttttcc ataacgacat aacgcggcat aacgtcttta atgatacgca
    52981 aatattcatg gaagagcatg tttctagggt ctttagaatc tcttttccct agacggctaa
    53041 agccttgaca aggaggacca ccaaaaataa catctacaga acctctttca aagatatttc
    53101 catatttcaa tgagtttatt gattgaaata ttttgtcaga agtaagttct tttatatcag
    53161 caagttcaaa gtgagtgtca accccgtcaa ctaagcctaa ttgttgatgt cggtttgtat
    53221 atgttttttc taccataggg cttttatcgc tggagaagat aatatcgaat ccagcttgta
    53281 ggattccttc gctaaatcct ccagcgccac agaataagtc tattgcatag ggcataattt
    53341 acctcgaatt ttgtatttga taccttagta gtatatcaga aaattattga aaagtccagg
    53401 agttactccc ctataataga tattcgttaa atatttgaga aagccagtaa aaatgtaaac
    53461 gaaaatgaat tttatttatg tttaaagtta taaaataaag aaaatgataa ttggagaatt
    53521 gttatggagc gttatatcaa agaaaatatt gagcgattac tttattcgag taaattatcg
    53581 caaagtgagt tatcgaaaaa gacaggactt agcaggcaga cgattaacaa attattatct
    53641 ccaaatacta attatagtcc taaactttca accttggtag tgattgctaa tgctttatca
    53701 atcaattttc cagaactttt gattagaacc gacaagataa gtgcttccaa agaatttttt
    53761 tctaattctg tggaggaata tcaaaatatt cttatacaaa atatcaagct atatttgaga
    53821 agatggaatc aa
//
LOCUS       DAVGJJ010000003        53442 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000003 DAVGJJ010000000
VERSION     DAVGJJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.003"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(208..570)
                     /locus_tag="VVE35_000136"
     CDS             complement(208..570)
                     /locus_tag="VVE35_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU3060403.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            578..658
                     /locus_tag="VVE35_000137"
                     /pseudo
     CDS             578..658
                     /locus_tag="VVE35_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(885..1043)
                     /locus_tag="VVE35_000138"
                     /pseudo
     CDS             complement(885..1043)
                     /locus_tag="VVE35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1316..2005)
                     /gene="rplA"
                     /locus_tag="VVE35_000139"
     CDS             complement(1316..2005)
                     /gene="rplA"
                     /locus_tag="VVE35_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU3060404.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRSGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2212..2637)
                     /gene="rplK"
                     /locus_tag="VVE35_000140"
     CDS             complement(2212..2637)
                     /gene="rplK"
                     /locus_tag="VVE35_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU3060405.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2769..2906
                     /locus_tag="VVE35_000141"
     CDS             2769..2906
                     /locus_tag="VVE35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060406.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDVVS
                     R"
     gene            complement(2946..3662)
                     /locus_tag="VVE35_000142"
     CDS             complement(2946..3662)
                     /locus_tag="VVE35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU3060407.1"
                     /translation="MKKRYLILTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGVAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VISTNGELVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(3665..4135)
                     /locus_tag="VVE35_000143"
     CDS             complement(3665..4135)
                     /locus_tag="VVE35_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU3060408.1"
                     /translation="MCLICQRIELIKAGENPYFVKELETGYVVIGDHQYFAGYSLFLA
                     KEHVTELHHLEKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRQ
                     RGDMNGYGLNGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSLEVDKLLEIKE"
     gene            complement(4137..5174)
                     /locus_tag="VVE35_000144"
     CDS             complement(4137..5174)
                     /locus_tag="VVE35_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU3060409.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDIKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLYDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSELVD"
     gene            complement(5272..6597)
                     /gene="brnQ"
                     /locus_tag="VVE35_000145"
     CDS             complement(5272..6597)
                     /gene="brnQ"
                     /locus_tag="VVE35_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU3060410.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDTNKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6768..6860)
                     /locus_tag="VVE35_000146"
     CDS             complement(6768..6860)
                     /locus_tag="VVE35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3060411.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6903..7055)
                     /locus_tag="VVE35_000147"
     CDS             complement(6903..7055)
                     /locus_tag="VVE35_000147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3060412.1"
                     /translation="MKEIAFDAFYQLYQNDQFSLVDVREVDEFAALHLEGAYNLPLSQ
                     LADSYD"
     gene            complement(7215..7793)
                     /locus_tag="VVE35_000148"
     CDS             complement(7215..7793)
                     /locus_tag="VVE35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU3060413.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     DFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7847..9247)
                     /gene="pepV"
                     /locus_tag="VVE35_000149"
     CDS             complement(7847..9247)
                     /gene="pepV"
                     /locus_tag="VVE35_000149"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU3060414.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9260..9865)
                     /locus_tag="VVE35_000150"
     CDS             complement(9260..9865)
                     /locus_tag="VVE35_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU3060415.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10036..10836
                     /locus_tag="VVE35_000151"
     CDS             10036..10836
                     /locus_tag="VVE35_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3060416.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11014..11847)
                     /locus_tag="VVE35_000152"
     CDS             complement(11014..11847)
                     /locus_tag="VVE35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU3060417.1"
                     /translation="MNHKIAILSDIHGNVTALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11837..13681)
                     /gene="uvrC"
                     /locus_tag="VVE35_000153"
     CDS             complement(11837..13681)
                     /gene="uvrC"
                     /locus_tag="VVE35_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU3060418.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNKP"
     gene            complement(13720..14421)
                     /locus_tag="VVE35_000154"
     CDS             complement(13720..14421)
                     /locus_tag="VVE35_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU3060419.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(14500..15732)
                     /gene="murN"
                     /locus_tag="VVE35_000155"
     CDS             complement(14500..15732)
                     /gene="murN"
                     /locus_tag="VVE35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU3060420.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15737..16957)
                     /gene="murM"
                     /locus_tag="VVE35_000156"
     CDS             complement(15737..16957)
                     /gene="murM"
                     /locus_tag="VVE35_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU3060421.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17069..17848)
                     /locus_tag="VVE35_000157"
     CDS             complement(17069..17848)
                     /locus_tag="VVE35_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU3060422.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRETNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQDFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17917..19578)
                     /locus_tag="VVE35_000158"
     CDS             complement(17917..19578)
                     /locus_tag="VVE35_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU3060423.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQVFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19753..21975)
                     /gene="recJ"
                     /locus_tag="VVE35_000159"
     CDS             complement(19753..21975)
                     /gene="recJ"
                     /locus_tag="VVE35_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU3060424.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIGANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEAWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEKLSDLSQVLEDYVREKGADAVGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22252..23010
                     /locus_tag="VVE35_000160"
     CDS             22252..23010
                     /locus_tag="VVE35_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3060425.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYTHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDMTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23050..23814
                     /locus_tag="VVE35_000161"
     CDS             23050..23814
                     /locus_tag="VVE35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU3060426.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23827..24503
                     /locus_tag="VVE35_000162"
                     /pseudo
     CDS             23827..24503
                     /locus_tag="VVE35_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            24513..25172
                     /locus_tag="VVE35_000163"
     CDS             24513..25172
                     /locus_tag="VVE35_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3060427.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKIKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25430..26619
                     /locus_tag="VVE35_000164"
                     /pseudo
     CDS             25430..26619
                     /locus_tag="VVE35_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
     gene            27022..28328
                     /locus_tag="VVE35_000165"
                     /pseudo
     CDS             27022..28328
                     /locus_tag="VVE35_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(28365..29246)
                     /locus_tag="VVE35_000166"
     CDS             complement(28365..29246)
                     /locus_tag="VVE35_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU3060428.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(29387..30715)
                     /gene="vncS"
                     /locus_tag="VVE35_000167"
     CDS             complement(29387..30715)
                     /gene="vncS"
                     /locus_tag="VVE35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU3060429.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(30712..31368)
                     /gene="vncR"
                     /locus_tag="VVE35_000168"
     CDS             complement(30712..31368)
                     /gene="vncR"
                     /locus_tag="VVE35_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU3060430.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(31417..31500)
                     /gene="pep27"
                     /locus_tag="VVE35_000169"
     CDS             complement(31417..31500)
                     /gene="pep27"
                     /locus_tag="VVE35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU3060431.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(31680..33059)
                     /gene="vex3"
                     /locus_tag="VVE35_000170"
     CDS             complement(31680..33059)
                     /gene="vex3"
                     /locus_tag="VVE35_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU3060432.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(33111..33758)
                     /gene="vex2"
                     /locus_tag="VVE35_000171"
     CDS             complement(33111..33758)
                     /gene="vex2"
                     /locus_tag="VVE35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU3060433.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(33771..35048)
                     /locus_tag="VVE35_000172"
     CDS             complement(33771..35048)
                     /locus_tag="VVE35_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060434.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(35405..35575)
                     /locus_tag="VVE35_000173"
                     /pseudo
     CDS             complement(35405..35575)
                     /locus_tag="VVE35_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(35718..36317)
                     /locus_tag="VVE35_000174"
     CDS             complement(35718..36317)
                     /locus_tag="VVE35_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060435.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(36454..37338)
                     /locus_tag="VVE35_000175"
     CDS             complement(36454..37338)
                     /locus_tag="VVE35_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU3060436.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(37342..37728)
                     /locus_tag="VVE35_000176"
     CDS             complement(37342..37728)
                     /locus_tag="VVE35_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU3060437.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLYMTENLERLESLVSWCIQKVEG"
     gene            complement(37721..39064)
                     /locus_tag="VVE35_000177"
     CDS             complement(37721..39064)
                     /locus_tag="VVE35_000177"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU3060438.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(39151..40035)
                     /locus_tag="VVE35_000178"
     CDS             complement(39151..40035)
                     /locus_tag="VVE35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060439.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEILLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(40047..40664)
                     /gene="cysE"
                     /locus_tag="VVE35_000179"
     CDS             complement(40047..40664)
                     /gene="cysE"
                     /locus_tag="VVE35_000179"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU3060440.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(40680..42893)
                     /gene="pnp"
                     /locus_tag="VVE35_000180"
     CDS             complement(40680..42893)
                     /gene="pnp"
                     /locus_tag="VVE35_000180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU3060441.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLTREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(43578..44444)
                     /gene="metF"
                     /locus_tag="VVE35_000181"
     CDS             complement(43578..44444)
                     /gene="metF"
                     /locus_tag="VVE35_000181"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU3060442.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNSISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIVDLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(44508..46757)
                     /gene="metE"
                     /locus_tag="VVE35_000182"
     CDS             complement(44508..46757)
                     /gene="metE"
                     /locus_tag="VVE35_000182"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU3060443.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLNLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSFVAAYQEVFAKLADLGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLFQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAD
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEVAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(47018..47284)
                     /locus_tag="VVE35_000183"
     CDS             complement(47018..47284)
                     /locus_tag="VVE35_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU3060444.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARHEQGINQRKLEEL
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(47368..47856)
                     /locus_tag="VVE35_000184"
                     /pseudo
     CDS             complement(47368..47856)
                     /locus_tag="VVE35_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            48012..49013
                     /locus_tag="VVE35_000185"
     CDS             48012..49013
                     /locus_tag="VVE35_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU3060445.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(49081..51486)
                     /locus_tag="VVE35_000186"
     CDS             complement(49081..51486)
                     /locus_tag="VVE35_000186"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU3060446.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKTIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYA
                     KGILEALFTRLGLAVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(51563..52072)
                     /locus_tag="VVE35_000187"
     CDS             complement(51563..52072)
                     /locus_tag="VVE35_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060447.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(52072..53118)
                     /gene="pheS"
                     /locus_tag="VVE35_000188"
     CDS             complement(52072..53118)
                     /gene="pheS"
                     /locus_tag="VVE35_000188"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU3060448.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT        15168 a        12138 c        10017 g        16119 t
ORIGIN      
        1 attattagca aggcttctac tgattataac ctaattctta gactacttca agactaactg
       61 tccaataata atctacagat aaatcatcca ctcttaatca aattttattc aatccatcta
      121 ctcattccca aaaggttgct gtataatgca agacattttt cttactgctt ttacttttcc
      181 ttaccctcaa tttagtgagc taataaatta ttcttcttca aaaggttgag atttatctag
      241 taactctaaa acattaatgg taggtagcaa cattgcttca ctgctatcaa ttgccgaaac
      301 tattgaaaca atagaacgta aataaggata aagaatagca gtaccattta cgtatagaat
      361 atcggtgata ttttcatcta tatttatttc aaaatatcct attacagtta cagatatttt
      421 tttcatggca tctttgtcaa ctaaagaaac actaagtttt acggctccat ggctataatc
      481 atctgtatga ccaaccttaa ctatgggata taatatatcg aaggtaaatg tgaacaaact
      541 gattagggaa tttaaaccaa cttctaacaa tgtgttatac tcaatgaaaa ccaaagagca
      601 gactaggaaa ctagccgcag gttgctcaaa acactgtttt gaggttgtag atggaactga
      661 cgtagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagtaacca
      721 tacgcacggc aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt attagaaagc
      781 tcgatgaact attccagatt cattccacta aatttcgaaa tcgagtaggg gataatctct
      841 agcccctctc acaccaccgt acgtgccgtt cggcatacgg cggttcaact aacttttaac
      901 gcatgtcgtt caaggtaata atccaaacac gaaaccagtc cacgtttttc caggactggt
      961 tttgatatag cacgtttaag taccgacttc tgagctacta attgatagtg gtcgccccag
     1021 ccagatacct tatccgctat ataggcctta ttatcacgtt cctgttcgta gagccacgat
     1081 ttcgctatcc cttcctctga cgattcttat ttccagcgtc agacttaaaa cggtctatcc
     1141 ccagaccgtt ttaatccgct acctcacgat agtcagactt gggaatcgct attgggttca
     1201 ccggtagtag gtgcccaccg aggacttaca cctcagatat acgacatgcc cgtcgtacat
     1261 caaaaaaagt tgagacatag tgtcttcaac ttttttacat tactttaact actgattaaa
     1321 gtgagtttac gtcaactttg ataccaacac cttgagtagt tgtgatagtc aagtttgtta
     1381 cgtaagttcc tttagctgta gctggttttg ctttttggat tgtttcgttg aaagctttaa
     1441 agttttcaac caatttttca gcttcaaatg atactttacc gatgattgct tgaacgttac
     1501 ctgaacggtc agcacggtaa gtgattttac cacctttaga ctcttcaact gctttagcaa
     1561 catccattgt tacagtacca gttttagggt ttggcatcaa gttacgtggt ccaaggacac
     1621 gtccaagacg tccaacaaga gccatcatat caggtgtagc gataactacg tcgaagtcca
     1681 accaaccgtc gttgatttta gcaacaaggt catcttcacc aacaaagtct gccccagcag
     1741 cttttgcttc ttcagctttt gcaccacgtg cgaaaacaag aacgcgtgaa gttttaccag
     1801 taccgtttgg caataccatt gctccacgga tttgttggtc agctttttta acgtcgatgt
     1861 tcaagttgta agcaacttct acagttgcgt caaattttgc aaagttagtt tcttttgcaa
     1921 gtgctacagc ttcttctacg ctgtatgctt ttgtgctgtc gattttctca agagcagcac
     1981 gaagttgttt gcttttttta gccattttct attctccttg taagtggttc aatcgatttt
     2041 catctcccac gtcactcctc gaaatgatga agtcttgcgg gttttcagtc tattagattc
     2101 ttttcctacg tcgttaagtt cccttgctgt acccaagtac aagcatcagt cacttgccta
     2161 gtatgaaaag caactaatag actgctgtga gattttctac tgtgttattg attagtcaac
     2221 aacagtgaat cccatagaac gagcagtacc ttcgatcata cgcattgcag actctacgtt
     2281 tgctgcgttc aaatctggca tcttagtttc tgcaatttct tgtacttgtg cacgagtaac
     2341 tgtagcaact ttagttttgt taggtgtacc tgatcctttt tcaacacctg cagctttttt
     2401 caaaagaaca gcagctggtg gtgttttagt gatgaaagta aatgatttat cttcgtaaac
     2461 tgagataaca actggaataa tcataccagc ttggtcagct gtacgagcgt tgaactcttt
     2521 tgtgaatccc atgatgttga taccagcttg accaagagca ggtccaaccg gtggagctgg
     2581 tgtagcttta ccagcaggga tttgcaattt tacaagtttt tcgacttttt tagccatttt
     2641 taaatcctcc tttgtggttt tggcggtaat tagagatttt tacctcccac aagtatgctt
     2701 tacacatacc catccattat acactattta tctagaattg caagagaaaa gtttattttt
     2761 tatactcaat gaaaatcaaa gaacaaacta ggaagctagc cgcaggctgc tcaaagtaca
     2821 gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtga
     2881 atagaaccga cgtagtcagt aggtaaggcg acgctgacgt ggtttgaaga gattttcgaa
     2941 gagtattaat cgacgtaatc tccgccttca aatccatagt attcttccaa actgagacca
     3001 ctctcagcaa tttctttagc ttcttttcca acataacgaa ggtgccattc ctcagccata
     3061 tagcctgttt ccttttcctt acctttaaga taacgaacaa caaagccata atcagccgca
     3121 tgatccaaga gccattgggc tgctttttct tcagttacca attctccatt tgtgctaatc
     3181 acatcaaagg ccaaacctgt ttggtgttcg ctgtaaccag gacgggcaga gtaacggtca
     3241 gccgctgcct tcccatcttg attgacataa tcttgataga gcttggtctg agtttcataa
     3301 cttctaaaac cactgtaatg gtcgctaatt gggaaacctg cctcttgcat cgctttgatg
     3361 agtttgacca actcagcctt ggctgttgga ttttcccctg gattgtagtc ttttgacaat
     3421 gggtagtgtt tattggctac gatgatttca tcgtatttcc cttgaatgct gtagtagtct
     3481 cctttgttaa ccacttctgc ttttttctgg gcaactcctt gagacttact gccgactgtc
     3541 ccatcagctt tggcagtttg ttctgtctta gctgcgccat cttcattttt tgctttttct
     3601 tgtgaacaag ctgctagact caaggctagc aaagctgtca agataaggta tctttttttc
     3661 atttctactc ctttatttct aatagtttat ctacttctaa cgataaacgt ttgactaatc
     3721 ttttaatctc atccggtttt gcttggcagg tttctgctgt catttcttca aagggaaccc
     3781 accatactgg accacgtcca ttgagaccat aaccattcat atcacctctt tgtctaggaa
     3841 acagatgcca atggagatga gcatcgccat ttcctagcaa ttcgatattc attttctcag
     3901 cagcaaaggc cttggcaact gcctcttgga ctagactcat ttcttcgaga aaacgtagtc
     3961 ttgtttcctt ttctaaatgg tgcaattccg tgacgtgttc cttggctaga aagagactat
     4021 agcctgcaaa atactggtga tctccaatga ctacatatcc tgtttctaat tctttgacaa
     4081 aatagggatt ttccccagcc ttgatgagct caattctctg gcaaataagg cacatatcaa
     4141 tccaccaact cactcttaag ataagcatca accatacgtt ctgttgcaac caccgagtca
     4201 atatgagtac gctcataaga atgactagac tcgatacccg caccaagaag ggcatgcttg
     4261 acctctgccc ctgcagacat agccgctgaa gcatccgaac cataaaatgg atagatgtcc
     4321 agcttaaaag gaatatcttg ctctttagcc aaggcaacca agtgttgacg gaagtcatag
     4381 tgataaggac ctgaagcatc cttgacacag atagatactg tgtactcatc tgtctgctgg
     4441 tcatctccca tagctcccat atccacagcc agatattcta ctacctgagc aggaatgtta
     4501 gaattagcac catgtcctac ttcttcaaaa actgaaaaag caaaatgagt tgttactggc
     4561 aattcaatct tctctttctt ataaatacga agcaggttga gcaaaatcgc tgcgctgacc
     4621 ttatcataca aatgacgaga cttgataaaa cctgtatcag tcacaacagt tcgtggatcg
     4681 aagctgataa aatcaccgac ctcaatccca agagcacgag tttctttttc attgcttact
     4741 tttatatcca aacgcacttc catattgtcc tgcgtgcgtt ctgcagttcc tgcatccttg
     4801 tagacatggc aagaagtttg gtggatgagg atggttcctg ataccttttg acctgtgcta
     4861 gccacatgaa cggtacagtt ttcaccttca atcatgttcc aaggaaaacc accaatacgg
     4921 tccaatttaa gacgaccatc tggtttgaca gcacgaacaa tagcacccag cgtatccaca
     4981 tgagcagtca catagcgatg ttgctcatca ttttgacctt tgatggtcac attgacacca
     5041 cccttagctg tgcgaactgg ctggtaacca aaaccttcta gagtcttgac taaataattc
     5101 gaaatttcac gagtaaagcc cgttggcgat gcgatggctg tcagttcttt gatatattct
     5161 actgtttgat tcatttcttc acctctttcg ctacctatta taacacattt atcatcggat
     5221 aaaaaaagaa aatcactcct gtagacttgg ctacaaaagt gatttaagtg attattccat
     5281 ttcaaaaaca tcgctttctt gcttgtttgg tagaaccaat gagagcaaga ttccaacaat
     5341 ggctggcacc aaccatggga gagatgcctt ggcaaaagga agaacgctaa caagatttgc
     5401 aagaaactca accttaaacg agcttcctag tacgcttgca atggcaataa ctgtaaccac
     5461 agcaattgtc aactgcatac ctggttttga aagggcaaca aatttgttga caatgacaat
     5521 cataacgata gcaatcgtga ttgggtacaa gataaccagt actggaattg agtacttgat
     5581 aatcgcatca agacccaaat tggcaatagc aaatccaatc aaggtaaagg ctgtcgcata
     5641 aaccttgtag ctgatttgtg ggaagcgctc attaaagaac tcagctgttg acacaatcaa
     5701 accaacagtc gttgtgaagc aggttacggt aaccatagct gcaaggaaga gttgagctgt
     5761 tgagccaaag atttcttgag tggcttgtga caagatgtaa acacctggtg ttccaccctt
     5821 catcgcttca gctggtactg ggaaatgatt tccaagaaaa cctaaaccga tgtaaagagc
     5881 gctgaaggca agggcaacaa cgataccaac aacccaaata gttgaaatgt attctttctt
     5941 acttgaaaat ccaagttgtt tcaaggtttg aactgcgatt acgctaaagg ccactgaggc
     6001 aagggcgtcc aaggtattgt aaccttctag gaaacctgta ccaaaggcag aagcttgata
     6061 agcagctgaa gcagcttgag gacttgttcc accatatttg atagctccca gaacgaccaa
     6121 gataacaatc aaaattgcaa agactggcgt taaaatacgt ccaatgcggt ctaagatttt
     6181 tgatggatta agcgaaatca aataggctgc cgcaaaatac agaaccgtaa atacaatcaa
     6241 gccaagtcct ttatttgtat ccgacaaaag ggggctaatc cctacttcgt aagctgttgt
     6301 agcagtacgt ggggtagcaa agaatggacc gattgacaag taaagaactg agaggtaaag
     6361 agtcgcaaac caaggcgcta tcttcgttga aatctcgtag atatatcctt taggatttag
     6421 cgttccaata ataagggtca agacggcgat accaacgcct gaaaagacaa aacctgcgat
     6481 ggcaggaaga aaatgttctc cagatagagc acctagagaa ggcggaaaaa tcaagttccc
     6541 cgcaccaaaa aatattccaa acaggagcaa acctgttagg gtaccttttt tagccataaa
     6601 aatctccttc atatttataa aatactgacc tagtatacca cgattaggag tctgaaaaaa
     6661 gcatcacgag gcaaatatta aatgttttca attttcttaa aaataagaat tatctaaaaa
     6721 taaaactctc ctacccagtc cacaccaagt aggagaaatg caaaatttta aagttcttca
     6781 aaggctaaca tgccaccctg gacattgata acattataac cttgttctaa taggaattga
     6841 caagcacgcg ccgatctcat tccagatttg caaataatat aatgcaagcg atctttgtcc
     6901 aattaatcat aactatcagc caattgacta agcggtaggt tgtaggcacc ttctaaatga
     6961 agagctgcaa actcatccac ttctctcaca tccactagag aaaactgatc gttttggtaa
     7021 agctggtaaa atgcgtcaaa ggctatttct ttcattcttt ttcttctaat actcttcgaa
     7081 aatctcttca aaccacatca gcgtcgcctt accgtgcgta tgttaccgac ttcgtcagtt
     7141 ttatctgcaa cttcaaagct gtactttgag cagcctacga ctagcttcct agtttgctct
     7201 ttgattttca ttgactataa aatggtttta attctttttt tcaaatctgg cactacttct
     7261 gcctcaaacc aaggattttt ggccatccag atttgatttc gtggtgatgg gtgaactagc
     7321 ggaaaatagg ctggcagata gtctttatag tgtttcaccc tctccgttac cttcccactg
     7381 attttctcct gtaaatagta ggcttgggca tattgcccaa tcaagagggt taactgaata
     7441 tcaggcaatt cctgtaagag ctgcggatgc catttttctg caaaatctgt acgaggcgga
     7501 agatcacccg acttgccatg tcctggaaag tagaaatcca taggcaaaac agcaaaataa
     7561 cctgaattgt aaaaggtatc ttcatccaca cctagccagt cccgcaagcg gtcaccactt
     7621 ttatctttcc agtaaaggcc tgcttcttga gttttaagtc ccggagcctg accgatgata
     7681 ttgatgcgag cagtttttgg cgctgcaaag agaggctcaa tgccacgctc tgtatagctg
     7741 gcattctgcg aatccgccat gattgcctgt ttgattcttt cgatttgaga catctacttt
     7801 ccctccaaaa agaagtccaa gcataagatc tcagacttct atcgctttat ttgatcaatt
     7861 cgtaaatagc ttcggcataa attgctgctg ctcggaagag atcgtccaag gcaataaatt
     7921 cattggcttg gtgcatggta tcaattgagt ctgggaacat agcaccatag gcaactccgc
     7981 gctctaacaa gcgaccaaag gttccaccac cgatgacttg ttcatgacct ttaaggcctg
     8041 tttgtttttc atagacattc aacaaggttt gcacaagtgg atcttccatt ggcacatagt
     8101 gaggggtatg accatgttct gatagactga cagaagcaac tggcaagttt tctaggattg
     8161 acttgatttg ttctggactt gttccttttg gatagcggat gttaagggca atggtattat
     8221 cagcacttgt ttcatcgaag cggaagacgc cggcattcat agaaagagca cccatctttt
     8281 catccacatg agcaatcttg agattttcac cctcatgatc gttcaagaga attttacctg
     8341 cgatgtcaag gtagtctttg gctggaccag caaagccaaa ctggctgagg aagagggcaa
     8401 ggtaagttgc gccattgaca cctgaagcag gcatagcacc gtgggctgat ttaccaatga
     8461 tcgtcacctt gtattggcca gcttcttctt ggagttctcc tctaagtttg tgttctgcaa
     8521 caaaggcatc tagtttagct tgcaagtctg tcaagtcacc tgaaacgact gctgttgctg
     8581 attctggtac catattttca cgtaaaccac ctgtaaagct gtgaagacgg gctgcacctg
     8641 tattttcacc tgcaaagtgg aggtattccg tgatgtttcc tttttcacca ttgatgattg
     8701 gaaattcagc atctggtgag aaaccaaagt ctggcttggc gagtcctacg tgctcgaagt
     8761 agtagtccat atctgcccag cctgattctt cgtctgttcc aacgatgaag cgaactttct
     8821 tagaagttgg aaggcccaat tctttgatga ttttcaaacc atagtagcaa gctgttgtag
     8881 gtcccttatc atctgaagca ccacgcgcat aaaggcgacc atctttgata gttggtgtgt
     8941 aagggtctgt gtcccaaccg ctaccagcag gcaccacatc catatgggca aagattccga
     9001 gaacttcttc tccatcacca aactcaaaat gtcctgcgta gttatcgaca ttcttagttg
     9061 ggtagccatc gcggtctgcg atttcaagga atttctccaa ggcttttact ggaccaggtc
     9121 caaatggatg ctgggcatca gccttgctat catcacgttc tgagttgatt tccaaaaggc
     9181 taaacaagtc agccaagagg tcttctttgc gtttttctac ttctgctgta aaatcaattg
     9241 ctgtcatttt ttcttctttc tatcttttct cgatgatttc atctactggc aagcggtagc
     9301 ttggttccaa tttgtcgtct gtgtaaccca ctgtgatcaa gagttctggg cggaaacggt
     9361 cttcgatttc caaaacttca ttaacttttg atttgtcaaa accaaggata atattggaac
     9421 caattccttg gtctgtaaga gcgagaacca agttcatagc aaccaaacct gcattgagag
     9481 ctaggtagtc gctgacttgt tgctcattgt aacgggcaaa ctcagctggc aaatttttca
     9541 tgaagtattg aagttgttct tctgagaaat tgttagcacc accaactcgg gcaatcttac
     9601 gagcgcgttt ggcaaggtct gtatctgtaa atagggcaat ggttacaggt gctgatgata
     9661 cctgctcaaa gtttgaacca taagccaact ttgccagttc agcatttttc tcacgaacaa
     9721 ctacaaattt ccaaggctgg ctgttgtggg cgcttggcgc caaggttgca atttcgatag
     9781 ccgtacgcac atcttttgga tcaacaggct tatcagtaaa atgcttagtc gcatgacgtt
     9841 ttttatttaa ttcaagaaat ttcataatcg atttcctttt ctaattctac tgcttccatt
     9901 ctaccataat ttgaaagagc ttgcagggat ttttcatgat taagtttttc tatcacagac
     9961 ataaaagata tatattggtt tatcaggaaa gtttctgata aactagcaag tactttacat
    10021 ttgaatggag acagtatgaa aaaatttagc ctattactag ctatcctacc atttttggtt
    10081 gcctgtggga atcaagctac acccaaagag actagcgctc aaaagacaat cgtccttgct
    10141 acagctggcg acgtgccacc atttgactac gaagataaag gcaatctgac aggcttcgat
    10201 atcgaagttc taaaggcagt agatgaaaaa ctcagcgact acgagattca atttcaaaga
    10261 accgcctggg aaagtatctt cccaggactt gattctggtc actatcaggc tgcggccaat
    10321 aacttgagtt acacaaaaga gcgcgctgaa aaatacctct actcacttcc gatttccaac
    10381 aatcccctcg ttctcgtcag caacaagaaa aatcctttga cttctcttga ccagatcgct
    10441 ggtaaaacaa cacaagagga taccggaact tctaacgctc aattcatcaa taactggaat
    10501 cagaaacaca ctgataatcc cgctacaatt gatttttctg gtgaggatat tggtaaacga
    10561 atcctagacc ttgctaacgg agagtttgat ttcctagttt ttgacaaggt atccgttcaa
    10621 aagattatca aggaccgtgg tttagacctc tcagtcgttg atttaccttc tgcagatagc
    10681 cccagcaatt atatcatttt ctcaagcgac caaaaagagt ttaaagagaa atttgataaa
    10741 gcgctcaaag aactctatca agacggaacc cttgaaaaac tcagcaatac ctatctaggt
    10801 ggttcttacc tcccagatca atctcagtta caataatact cttcgaaaat ctcttcaaac
    10861 cacgtcagct ttatctgcaa cctcaaagct gtgctttgag caacctacgg ctagcttcct
    10921 agtttgctct ttgattttca ttgagtataa gatatataaa agcgattgga ctaccaatcg
    10981 tttttagttt gcattggtta ttttaggaaa ctcttacaaa aaatcaaaaa aattcttcac
    11041 atcctctaca tacccatgct tttctattaa gcttgctaat aattccagat tgtgcccctg
    11101 atagttatcg tcacgacgca gttcttcata catttcgata aagggaagtc ccttggactt
    11161 ggccaattct aactcagcct cgtaatcata agcaacttta cgaaatagga tatttaccaa
    11221 ttccccatct tcaacctcta tcacggcata ctgggcacgg tgatttttta acgcctccca
    11281 attaaaatag ggcatgccaa tcgaccctgg attgatgatt tgttgccctt gactaccata
    11341 acgaagcaac tgcttgtgaa catgaccata aactgccacg tccgtttccg catctagcag
    11401 ttggtcaaat ttctctgtat cattctcaac tagcaagtca ccaccatagt ttttgtcagg
    11461 taaattatga gagatagaaa agcgcaatcc gtcaatttct ttcttttcca gcaaaggcaa
    11521 gcttcgtagc cagacaatcg ttgcaggatc cattcgctcc atcaaaaact gggtcatgcg
    11581 cattgactgg atttctttcg gatattccaa accatattcg ccatctaaag cctcaaggac
    11641 acgatcatcc caattgcctc gaacacttgc tgtgatagga aggtccttta gcagggcgac
    11701 taagtcattt gcgcctggac caggaagaaa aatatctccc agaagccagt attcactgac
    11761 tccttgattt ttagcatctg caatcactgc ttctagcgcc gtcacattgc catgaatatc
    11821 tgataaaatt gcgattttat ggtttattgt ggtttccttc cgtttggtaa tctactcttt
    11881 cttctgctac ttgaggcaag gcttctccct gcgggttcaa ctttctttgc acagcctctg
    11941 caacgactct aggtacccca acttcgacaa tctcatccac actggcttcc ttgattttgg
    12001 tcaaagactt gaaatgcttc ataagatttt gcttgcgttt aggtcccaga ccatcaatcc
    12061 catccaattg agatgagaaa gaatttttgg agcgcagttg gcggtggaag gtgatggcaa
    12121 agcggtgcac ctcatcttgg atgcgttgga ggaggaaaaa ttcctgagaa ttgcgagaca
    12181 agtccaccac ttccagcgga tctccaaaga gcaattcatg ggtttggtgc ttatcattct
    12241 tttgcagacc tgcaattgga atatccaagc ccagttcttc ttggatgact tgcttagcaa
    12301 tattgacttg accttgcccc ccatcaatca caatcaaatc tggaggagtc aaagcctcac
    12361 gctgtactcg accatagcgt ctgcgaatga cctctcgcat actggcatag tcgtctggcc
    12421 cgactacggt cttgatttta tacttacggt aatccttctt actaggcttg ccgttgacaa
    12481 agaccaccat ggctgaaaca ggactagttc ccatgatgtt ggagttatcg aaggactcga
    12541 tgcgaactgg ggttgggatt tgaagcaagc gtcctagatt ttcaatagct ccttgggtct
    12601 tctcgacaga tttctctagc aggttgaact tctgctctag gctaacacgg gcatttttta
    12661 tagctaaatt gaccagctgc tttttctctc cacgttgagg cttaagaatc ttggaatcca
    12721 ccaaggcctt gacagcctct tcgtcaatat cctgcggaat cagtacctca ttgggaacta
    12781 ggtgagattt ttcttgatag aattgtccca cataggtcaa gaaatcctca tctggatcat
    12841 tgaagtaggg gaagagattg acatcgcgct cgatgagctt gccctgacga acaaagaaaa
    12901 cctgcacaca catccagccc ttatccacat agtagccaaa gacatcgcga ttttggagat
    12961 ccttagccat gacccgttgc ttggttcgaa gcgttccaat agcctgaatc aggtcacggt
    13021 attccgccgc acgttcaaac tccatagtct gggctgccgc tgccattttg ccctttagct
    13081 catcaatgat tttgttatcc tgccctttca gaaaatcaga aacctcctgg gccatagact
    13141 tgaaataagc ctcatccttc ttacagatgg tgtgagccat acactggccg atatggtaat
    13201 aaaaacagac cttagagggc gggttggtac acttacgaaa agggaatatc cgatccagca
    13261 accgcttgat ttcattggct gcccccacat cgggataggg tccaaaataa agacctccgt
    13321 cctttttgac ctgacgagtg ataatcaagc gtggatagcg ctcattggtg attttgatga
    13381 aaggatagga cttgtcatcc ttgagcatga tattgtactt gggcttgttt tccttgatca
    13441 ggttgatttc taggagaagt gcctcaatat tagactccgt aacaataaat tcaaaatcca
    13501 caatttcaga caccaaggcc tctgttttgg tatcatgact tccacggaaa taggaccgta
    13561 ctcgattacg cagattttta gcctttccta catagataat ggtgccgttt ttatccttgt
    13621 aaatgtagca accagggctg gtcggcaaga gctctagttt tggtttaatc aagttgttca
    13681 tagttccatt atagcaaaaa agtagggaaa gatagtaccc tactcattag tctcatatat
    13741 ttttcggagg tttgcaacat cctcttcctg gataccataa aaggaacctg ctggttgcat
    13801 gacagacttc tcatctgtat ggtccaagcc aatcgcatga cctaactcat gttctgccgt
    13861 atggacaagg cgttcatagg aatagccata gtatggattg gacaaataat aatgattcaa
    13921 ccgcaccgtt acggacaaga attgccctgt taagagatta gtttgacttt ccgcctctcc
    13981 tgccacagga gtgcctccgt cgttcatctc cgtagccgta atatccgcct tactagactc
    14041 agtcacgagt tcaaagttaa aagcaccagt ttgattccag ttctggattg cttctaaata
    14101 agcctcttga aagcgtgaat ccatctgcgg atccaggtaa atacgagcag aggcctgtgg
    14161 ccaccttcct tcagaatgct ggtgactatc tgtctggctt aacttaggca gttgccctgt
    14221 tttctcccat tggtcaatac tttgttgacc aatttttact gaccgttctg cttgctttag
    14281 cgctccttga aaatccccgt tcagatacca gagaagtccg aaagcaagca cacataagag
    14341 cacaactatc caaaccagac gccaaaacaa acgccacaca aaagaaaaga aagcccctat
    14401 caaacgaaaa agccagcgca tgtctctcca cctttctagc aaataaaaat tattttacta
    14461 aaagaagcta aaagaaatct aaatactgac tttttcatct taacgtccta ctattttttt
    14521 gagtaactgg atagctttgt attttaaagg agatggatgg taacggaaag tacctgcttt
    14581 gcgtacaata tagccattaa aattctgttt aaaacgcaaa acaccatcac ttccatcaaa
    14641 aatcccttga atgcctagga aattgtattt aggtattcca cgttttatgc tttccaacat
    14701 aacatatttt tgaagcagtg caggggcata gaacttatta aactcagtgt aggaaccact
    14761 aaagagataa gtcgtttcct gaggcatata aacaaataaa ctcccagcta aaacaatatc
    14821 ttcttctcca tatttttcaa tcaagtctcg cgcttctgct tttcgaactt caaacgtttc
    14881 aaattgacta gaatattctc tcagttgatt ttgttttttc tcagaatgag gatttttact
    14941 caaatcaagt cgcaacttgt ccaagttttc ttctagttta ttttgttcac cttgcaattt
    15001 gctcatatag tccgaaaaat ttaagcttgc tatgagaaac tccgcttgtt ctccaaaagt
    15061 atcataaaaa tgctcataat attctaaact tttatcacta tattctctac gttcagaggt
    15121 ttcttttgtt atattcttaa aaatcgatag ttcttcacgt tttaactttt tcaatcgaat
    15181 gccaaaggtt tcagcctttt tcaccaaggg tttacccttt ttgctaaaac ttttaagcaa
    15241 actcttttca gttaattcag ttaaatcttt atagtataac caatctggtt ctccacctgg
    15301 gtaacctgtt gttaagccat caaattgata acctaaatca gtcaaacctt gaataatact
    15361 ttttttctca gcatctattg gattaccttg gctatcaaaa gtttgataag tttcataagg
    15421 ttttacaagc aactctaata caccattttg cttggcatat tcttttaact ctgcataaaa
    15481 aactggaaga gcatcttgtt gggtataaat cggccccgaa ttgagttcca tatgcagacc
    15541 acccagcatg ggcaagctat aaaccagagc tgcaacttga atttctcctt cttgtttcaa
    15601 agcaagataa acaattcgag cccctctttt ttctagcaaa tcccccatct ggacagattg
    15661 cataaaggaa cgagaagaaa cctgatcaga ataagtctga aactcttctt tcgtgagtgt
    15721 tgttagtgcc atatacttac tttctatgtt tttttcttaa tgttttacgg aaatcaagag
    15781 caagtcttaa cagaggatag agaggatgag tgggcattgt aaattcaccc aagtattctt
    15841 caatcgttgg attaaatttt tccttaaaat gataaagtcc accattgaga gagttttcaa
    15901 caccacctaa attttgccag accatacctc gctcaaaggc atagcgagcc gtttcatacc
    15961 atgttaaaat tggtgcattg taacgtttaa aatcatcatc cataccagca tatatattga
    16021 cagaggtagt accaaattcc aaactcaaag tagccgctaa aggaactctc gcttgaccta
    16081 catctatata ttcctgcaag aaggtcaatt cctctaacaa acgttctttt tccttcttct
    16141 gcgcttctac ttttgaagtt cgagtcgact cagtaaaggt ctcttccaag gctctatttt
    16201 tcgctaactg ttcttctaat tcttgcgaac gtttagaaac atccaaggtg gccaaggtga
    16261 tataggcctt gtccttaaaa ttatctaaca attttttata ataggcttca ttcctcaaat
    16321 gaatctcttt tcgcttctca gtttttttca tcaactccga aaatgaatct aatagttcca
    16381 gtccaccata ttgaatctca agccctttgt ttcgtgctgt tcgaatagcc tgttttgttg
    16441 acttggaaag tttatcttct tcaaaatttt ccttgtatat tttcgcctga atacgaggtt
    16501 gaatggtgtc tcccatttcc tccgtttttc ctgaccacct tactcccatt tgttgcaaac
    16561 tatcaataat agccagattt tcaggaaatt ctgtcttttc ctgattgatt aaactttgag
    16621 ataggcaaat acttgggtca aaagtcacaa aaaccgctct cttactgcga gcataggact
    16681 taatagactg aatggcaaaa ttcaagagtt ctttatcccc ataatccaat ataggtcctc
    16741 ttggaatgta aaacatttta tagcctagcg gaagagttct aatcaaaata ctagctgtcg
    16801 ccagtaattt ttcttccctg taaacaccaa acttctcatg ttgccaatta gacttaactt
    16861 cctcccaagc actactttgt aatacattgg ctaattcatg ttctttgaca aactgatcat
    16921 attctaatgt gggaatgcca atttgataac ggtacatttc ctactctctt tcctccagta
    16981 ttttattact attctactac tttcgtttga aaagtctagt gaaaacatat tttattttag
    17041 ttctactccc cctatgctga agttcaaact aagtcagtct ctcttctaat ttgaaatcaa
    17101 ttggtagggt tgttaaaaaa acttccaatt gtttttccca gtagtaccac tcgtgagttc
    17161 cagcactatg gctataggtc acatcaaaac ctagtttttt gagatttttc actgcgagat
    17221 tattggcttc gtacaagaaa tcctgttcgc cacaccacgc ccaaagtttg gtctttttat
    17281 ccgatttttt agccagactt tcaagagaat agggactagt tgtccagtct ttaatctctc
    17341 caaaaacacc tctccagtag gctggacttc ccagattttg actttcagga gaaaaatctt
    17401 gaaagctcaa tgcacctgaa aagctagctg catgagaaaa acgatttgtc gcaagagcca
    17461 gtttgaagca gccgtagcct cccatagaaa gaccagcgat aaaggtcttt tcacgcttgc
    17521 tagtcatatt agggaagaag cgtttcagaa cctgtggcaa ttcctctgct agagccgtgt
    17581 agtagtcaaa accatactgg gtatcggtgt accaaccatt gctggtattg ggcataacaa
    17641 cgatgagatt agtttctcga agcaagcgtt ctacattggt ccgcttaagc caactattat
    17701 gatttccaga catcccgtgt aaaaggtaca agacgggaat atcttcacac tctggttctt
    17761 ccactcgatt ggcatcaggg tagaggacat tcacccccca ctccatatcc aatacttgtg
    17821 agtaatactc gattttcatt actgccatga ttttctcctt ctatttttct ataagaaaaa
    17881 gccgaaactc gactttctct ctgtttattt caacgattat tttgcttcca tgactactgg
    17941 taaaatagct ggacgacgct tggtttggtc aaagagatac ttggtcaaat tatctcgaac
    18001 cttcccttta agatctgccc agtcaaagtc atctccttga agatactctt ctaccgtttg
    18061 gttaatcaat tctgaacttt cacggagaat atcgcgactc ttcttgagat aaacaaatcc
    18121 acgcgtgtga acacgagccc tagccacaat tttcttctca cgacggttga ctgtaatagc
    18181 cacgatgaaa attccatcct ctgacaagac cttacggtca cgaagaacaa catttccaac
    18241 atcaccaatg gcattcccat caatcaagat atctcctgct gaaaccgatc cagctggaac
    18301 aaagtctcca ttctcgtaag ccatggtcgt ccccttttta ggaatgaaga tgcgttctgg
    18361 caacatccca actgccatgg cagccttagc gtgagcatcc aactcacgat actccccttg
    18421 gacagggaag aggtacttag gttgcaaaag attgatcatc agctgcaaat cacgcacatt
    18481 tccgtgccct gatacatgta aactttgggt aatcaatttg acaaccccac ctgcctgata
    18541 aatcatattt tctacacgcg caacaaaggc ttctttagca atagacggag ccgtagcaat
    18601 atagaccagg tccccatcct tgatttctac ataacgatgg cgaccaatcg acatcttacg
    18661 aagtccattg ataggttcac ccatacgacc tgtctcaaga ataatcaact catggtcttc
    18721 aaagcgagac atatctttag gcttaatcaa gagaatttcg ttggctaaag acaacttctt
    18781 aagacgaatc gctgtgcgga cgatattttc aatatcaaat cctgtcaaga cgatacgtcg
    18841 acctgtttta tccgcagcgt caaaaacctg ctgaatacga gaaagattac tggaaacagc
    18901 tgcaacgatg atacgacctt cccagtcagc aatagtttgg gtaatttcat ccctaacttc
    18961 actttcacta gccacctgaa tattgctgtc tgcattggcc gaatcactga ggagagccag
    19021 gacgccgtca cgaccaatct ctgccaaacg agcaaaatca gttgcataag attcactagc
    19081 cgtttggtca aatttgaagt cacctgtata aacgatgctt ccttccgatg tcttcaagac
    19141 aattcccaga ctctctggaa cggagtaagt cgtagggaag aaggaaacca ctgtcccacc
    19201 aaaatcaatc tccgtattct catcaatgac atggaaatca ttaaatttct taacggcatc
    19261 atttcctttg acaaagagct ttgccaactc aatggtcaac tcagacccaa atacaggaac
    19321 tttagcctct gccaagagat aaggtagagc accaatggca tccgcatgcc cgtgggtcaa
    19381 gaaaacccca gcaatacggt cgctattttc aaaaaggtaa tccatgtttg gaatcaccac
    19441 atcgacccct aattgttcat tttcaggata ttttaaccct acattcaaaa caaaaatgga
    19501 ctctccaatt tcagcaatgt acatattttt tccattctca cgcacaccac caagtgttgt
    19561 taaactgata ttactcattt ttcctccaaa atcttagttt atcttgatta tagggcaact
    19621 ctaccctctt attctaaaag ttctaatact ttcagccgaa tcttttccta ccattatacc
    19681 atttttttga tgattttcaa aatgaaggtc tgttttcaaa aaagagttga tttcccaact
    19741 ctttcctaac ttctactctt tttccatcaa aaaatcataa atttcttgca ccgtacccag
    19801 cgccatcatt tcttggtctt taacggtttg tttgagattt tggtaaattt gactttctcc
    19861 tatctcccgc tttggcgcct ctttattgac tgtcatcaca ccatctttta tcgtcacaaa
    19921 gcctagttct tcaaatactt gaatcatctt gaccagcaag atttgttgaa tattaagata
    19981 tgcagccaaa tctttcagct tgtagcgaat atcaaactct gggaactggt aaatagtctt
    20041 gtacaatttg gcaaactgat ctctagtccc ataacctgtc agataatagg ccttgtcaat
    20101 atcatttttg aaatagacag cagagaaatg ctgttcctga aaaatggtct tcagctgagt
    20161 aatatcctct ggaatgtttt ttacgacaac agcttcacta gccgcaagat ttggcagttc
    20221 tccaggaaaa tccaagactg gaacaccttc tggcaagact gcattttttc cacgaatgtt
    20281 aaaaagttga acaccttcca ctcgcgcatc catcatcatc aactggaggg cagtttggcc
    20341 gttccattgg ttgacagaca atttaaccgc taactctaga ttcttggttt gagaaaactc
    20401 tgtcgcccat ctgccttgac caaaggctac cacttcaaaa ctcgcctcac ccttggaaat
    20461 tttcagcttt agatgggcat tacctgcccc catagtacga gcactttcga cctgaaaatt
    20521 cttgatataa aaaataggtt tctgattatc cattccaaaa ggagctaaac gttcaaaact
    20581 tttgaccgtt tccaagctaa gtgcctccaa atccagttct tcatctagat ttaacttgtt
    20641 cttgccaaca gcatctgcac ctttttcacg gacataatct tccaagacct gagataaatc
    20701 tgaaagtttc tcaacttcca gcgtcatgcc agctgcacca gcatggccac caaaggctat
    20761 gaagaggtct cgatgaggat ccaaggcttc aaaaatatcg accgcttcca cactacgagc
    20821 actgcccttg gcacgaccgt cttctatatt aagcacaatg actgtctgcc ctagttcttc
    20881 caacaagcgc cctgccacga ttcctagaac cccaggattc caggcttcct tagccaagac
    20941 ttggaccttc ttctcaggat ccaccatagt cttggcttct tcatagatag attgcacgat
    21001 ttccttgcgc tcttcgtttt tctgatgaat catgagggca atctcgtgcg cttcctcgtc
    21061 gtcaaatcca gtcaacaaat caatagcagg attaggatca tccaagcgac ccaaggcatt
    21121 caaacgaggg gcaatctgga aaccaaccgt ttcttctgtc acttcgttgg caccaatccc
    21181 agccatgtcc agcatttctt gcagaccaat gcgctgggta tgacccaaca tttccagacc
    21241 atattgaact aagatacgat tttcatccgt cagactcacc atatctgcaa tagttccaat
    21301 agcgaccaaa tcaagcaatt ccacttgcac ttcttctaac agggcacaag ccaacttgaa
    21361 agcaactcca caaccagcca aatatttaaa aggataatcc gcatctggat gttcaggatg
    21421 gacaatagca taagcatctg gcagggtttc aggcatggaa tgatggtctg tgacaatgac
    21481 atctactccc atagactgag ccaatgcaat agcctcatga ccagcaaccc cattgtccac
    21541 cgtcacaatc aaggaaatcc cttcttgctc gataaagtat ttataaacac tagcattagg
    21601 gccatagcca tcggtaaaac gatttggcag gtaaactcgg cactcagcac caagttgttc
    21661 caaactttcc ttcacaatag aagccgaagt catgccatcc gcatcatagt ctccataaac
    21721 gagaatattt tccccttctt caatagcctg acgaatccgc tccactgcct tgtccatatc
    21781 atggagcaga taagcatcat gtaagtcctc caaggaaggt tctaaaaact tcttcagact
    21841 ttcttggtcc tgaatccctc tctcaaacaa taaccgagcc acctcaggac ccagtccagc
    21901 cttcttggct atctttgtaa aatccgcatc ttctacctgc agggcaaact gccattcata
    21961 agtaggtgtt atcaaaaaga catccactct ttctaagaat tctattgctt cattataaca
    22021 tgatttagac gttttctcta tccagattgc ttttttataa cattttatag tatattgaaa
    22081 ctagaatagt acacctctac ttctaaaata ttgttagaaa tcgatttgac tgtcccgatc
    22141 gatttgtcct gttcttattt cactatagtg aattttttca gatatggttt gacaagaaca
    22201 ttcttttatt gtagaatcgt gttataaaat ctaacataaa ggagatctct catggcttta
    22261 gtagaattta aaaacgtcga aaaatattac ggagactacc acgcactccg caacatcaat
    22321 ctccgttttg aaaaaggaca agttgttgtc ctgcttggac cttctggctc tgggaagtcc
    22381 actcttatcc gtacgatcaa tggtttagag gctgttgaca aaggaagtct cctagtcaat
    22441 gggcaccaag ttgctggtgc cagccagaaa gatttggtac ctcttcgcaa ggaagtcggc
    22501 atggtttttc aacattttaa cctttataca cacaaaactg tgttagaaaa cgtgacactt
    22561 gcgcccatta aagttctagg aattgataaa aaagaagctg aaaaaaccgc ccaaaaatat
    22621 ctggaatttg taaatatgtg ggacaagaaa gattcctatc ccgccatgct atctggtgga
    22681 caaaaacagc ggatcgccat cgctcgtggt cttgctatgc atccggaact cctcctcttt
    22741 gatgaaccaa catctgctct tgatcctgag actatcggag atgttctagc agttatgcag
    22801 aaactggcgc atgatgggat gaacatgatc atcgttaccc acgaaatggg ctttgctcga
    22861 gaggttgcgg accgcattat ctttatggcc gacggagaag ttttagtaga tatgacagat
    22921 gtcgataact tttttgacaa tccaagcgaa cctcgtgccc aacaattcct cagcaaaatt
    22981 atcaaccacg aaagtgacaa agtcaaataa ggaggcgcct atgaaaaaaa attcttttta
    23041 tcagcattat tgattagcct tttcggcctt gctgctgcca aaccagtcca ggctgatact
    23101 agtatcgcag acattcaaaa aagaggcgaa ctggttgtcg gtgtcaaaca agacgttccc
    23161 aattttggtt acaaagatcc caagaccggt acttattctg gtatcgaaac cgacttggcc
    23221 aagatggtag ctgatgaact caaggtcaag attcgctatg tgccggttac agcacaaacc
    23281 cgcggccccc ttctagacaa tgaacaggtc gatatggata tcgcgacctt taccatcacg
    23341 gacgaacgca aaaaactcta caactttacc agtccctact acacagacgc ttctggattt
    23401 ttggtcaata aatctgccaa aatcaaaaag attgaggacc taaacggcaa aaccatcgga
    23461 gtcgcccaag gttctatcac ccaacgccta attactgaac tgggtaaaaa gaaaggtctg
    23521 aagtttaaat tcgtcgaact tggttcctac ccagaattga ttacttccct gcacgctcat
    23581 cgtatcgatg ccttttccgt tgaccgctct attctatctg gctacactaa taaacggaca
    23641 gcactactag atgatagttt caagccatct gactacggta ttgttaccaa gaaatcaaat
    23701 acagagctca acgactatct tgataacttg gttactaaat ggagcaagga tggtagtttg
    23761 cagaaacttt atgaccgtta caagctcaaa ccatctagtc ataccgcaga ttaaggagga
    23821 taccctatga cagatttatc atcttggaca gcctattttc aggattttgg acaatttttc
    23881 aatggtttcc tcttcaccct tgccctagcg attggatcct ttatcctcgc catggtctta
    23941 ggaatcttct ttggtgccat gtcaaccagt aaacgtccaa ttttacgcat tttagctcgc
    24001 atcttcgtgg aattttacca aaacactccc ctcttggtgc agtttgttat tgttttttat
    24061 ggcctacctc ttatcagtga ccatatcatc atgattccaa tttattggac agccgttctc
    24121 tgcgtgggac tctatcacgg cgcttatatc gctgaggtta ttcgttcagg gattcagtct
    24181 attcctagtg gtcagatgga ggccgccttg tcgcaaggtt ttacctatat cagtgccatg
    24241 cgcttgatta tcttgcctca ggccttccgc atcattctcc ctccattgac caaccaaatt
    24301 gttaacctca tcaagaacac atctactgta gctatcatct ctggagtaga cttgatgttt
    24361 gtgactaagt cttggtcggc tctcaacgga actatatccc agccttttta ggtgctgctc
    24421 ttctctactt tgccctatgc ttccctgttg cccagtttgg tcgcaagatg gagcaagtca
    24481 ataaaaaagc ctattcactt taggaggtta ctatggaatc tattttagaa attttaaccc
    24541 cagataacct agtctttatc tttaaaggat ttggcttgac cctctatatt tctctgattg
    24601 ccatcatcct ctctactatc atcggtacgg tgctagctgt catgagaaat ggcaaaaatc
    24661 ctatcttacg cattatttcc agtatttata tcgagtttgt gcgcaacgtt cccaaccttc
    24721 tctggatttt tactatcttt ttggtgttca aaataaagtc cacaccagca ggtattacag
    24781 cctttactct ctttacatca gcagccttgg ctgagattat tcgaggcggt ctcaatgccg
    24841 tagacaaggg acaatacgaa gcaggaatgt cacaaggctt cacctcagcc caaatcctct
    24901 actacatcat tctcccacaa gccatccgca aaatgctacc agccatcatt tctcagtttg
    24961 ttaccgtgat taaggatacc agtctcctct actctgttat cgccctacaa gaactctttg
    25021 gagccagcca aattctcatg ggccgttatt tcgaaccaga gcaggtcttc agtctttaca
    25081 tcctgattgc cctcatctac ttcagcttta acctagcaat ttctagcctg tctcatatgc
    25141 tagccaaacg ttggcaacaa gctgcagaat aatactcttc gaaaatctct tcaaaccacg
    25201 ttagctctat ctgcaacctc aaaacagtgt tttgagcaac ttgcggctag cttcctagtt
    25261 tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg agagtttttt
    25321 gatgatagta gatgtttata gacctcaatt tgacaccata cattttctat gataaaatgt
    25381 aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta tgaaatcagt
    25441 caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt ggatgaacac
    25501 ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga cttttatcct
    25561 agccactcgt ctcccactac tagaaagctg gtttctcggt ttggagaagg tctacaccgt
    25621 ccacaaattc acagcctttc tctccatcat cctactaatc tttcataact ttagtatggg
    25681 cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct atatctttgc
    25741 cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt ggcgatggat
    25801 tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca tgataatggg
    25861 caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg cccttttagg
    25921 cttactagct ggtttttata tcattttcta tatcaaaaga tttccttccc ctatctaggg
    25981 aaaattaccc atctcaaacg cttaaatcat gatactagag aaattcaaat ccatcttagc
    26041 agacctttca actatcaatc aggacaattt gcctttctaa agattttcca agaaggcttt
    26101 gaaagtgctc cgcatccctt ttctatctca ggaggtcatg gtcaaactct ttactttact
    26161 gttaaaaatt caggcgacca taccaagaat atctatgata atcttcaagc cggcagcaaa
    26221 gtaaccctag acagagctta cggacacatg atcatagaag aaggacgaga aaatcaggtt
    26281 tggattgctg gaggtattgg gatcaccccc ttcatctctt acatccgtga acatcctatt
    26341 ttagataaac aggttcactt ctactatagc ttccgtggag atgaaaatgc agtctaccta
    26401 gatttactcc gtaactatgc tcagaaaaat cctaattttg aactccatct aatcgacagt
    26461 acgaaagacg gctatcttaa ttttgaacaa aaagaagtgc ccgaacatgc aaccgtctat
    26521 atgtgtggtc ctatttctat gatgaaggca cttgccaaac agattaagaa acaaaatcca
    26581 aaaacggagc ttatttacga aggattcaag ttcaaataag tactcatact ctcctgggat
    26641 ttcccaggag agttttttat agccaaatga aataagaccg gaacgatgga attttggcat
    26701 tcaaattgat taggaaaatc aaatgaattt atagaaatct tttagcagtt atattgtcct
    26761 attctagttt caaaacgcta tagaagcagc attgtgctag tctagattca gtttactata
    26821 ctaaaacgag tagcttgaaa tcaaaaaacc caccctcaca caggcaggtt ttatctgtat
    26881 tattcagcta gattatgcgg ctctataata tttgtagtgg gtaaatccac tatagatatt
    26941 atggagccta ttttatgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa
    27001 caactcatta gaaagattca tatgggacaa ttacatttta tcacaaaact actcgatatc
    27061 aaagacacaa atacccaaat tatagatgtc gtcaataggg attcacacaa agaaatcatc
    27121 gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaagtcaaat gaagaaatat
    27181 gactttcaaa aaccgtctaa gattccttat cttgaaacga ctggtatgcc tactagaatt
    27241 ctccttagaa agcgttgatt caagtgctat cactgttcaa aaatgatggt cgctgaaact
    27301 tctatcgtca agaagaatca ccaaatccct cgtattatca accaaaagat tgctcaaaag
    27361 ttaattgaaa agatttctat gactgatatt gcccatcagc tggccatttc aacttcaact
    27421 gtcattcgca agctcaatga ttttcacttt aaacatgatt tttcttgtct tcctgagatt
    27481 atgtcttggg atgagtatgc ttttacaaag ggaaagatga gcttcattgc gcaagatttt
    27541 aacaatctca atatcatcac tgttcttgag ggtagaacac aagctatcat tcgaaatcac
    27601 tttcttcgct acgatagagt tgtccgatgt cgcgtcaaaa ttattactat ggatatgttt
    27661 agcccttact atgacttagc taaacagctt cgctttcaaa tttctaggct caggctgaaa
    27721 cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc tttcacattg
    27781 tacaacatct tagccgtgct atgagtcgtg tgcgtgttca aatcatgaat cagtttcatc
    27841 gaaaattcca tgaatacaag gctatcaagc gctactggaa gctcatacaa caggatagcc
    27901 gtaaactgag tgataagcga ttttatcgtc ctacttttcg tatgcattta accaataaag
    27961 agattttaga caagcttttg agctattcac aagacttgaa acaccactat cagctctatc
    28021 aactcttgct gtttcacttt cagaataaga aaccggagaa atttttcgga ctcattgagg
    28081 acaatctaaa gcaggttcat cctctttttc agactgcctt taaaaccttt ctaaaggaca
    28141 aagagaaaat cgtcaacgcc cttcaactac cctattcaaa cgccaaattg gaagcgacca
    28201 ataatctcat caaacttatc aagcgcaatg cctttggttt tcgaaacttt gaaaacttca
    28261 aaaaacggat ttttatcgct ctgaatatca aaaaagaaag gacgaaattt gtcctttctc
    28321 aagcttagct ttttttcaac ccactacagt tgacaaagag ccgattatgc tttaccttct
    28381 gaaccgaata cgtcgatacg ttcttcaact gatgcttgga tagcttttac accgtcagcc
    28441 aagaatttac gtgggtcgaa gagttttttc ttgtcgtatt ctgcttcgtt tgcttcgtag
    28501 tcacgagcaa atttacgagt tgcgttagcg aatgcgattt ggcattctgt gttaacgtta
    28561 actttggcaa caccaagttt gatagctgct tggatttgct catcaggaat acctgatcca
    28621 ccgtgcaata cgattgggaa tcctggaaga gcttctgtca atttttgcaa gtggtcaagg
    28681 tcaagacctt cccagtttac tgggtaagga ccgtggatgt taccgatacc agctgccaag
    28741 aagtcgatac cagtttcaac cattgcttta gcgtcttcga ttggagccaa ttcaccttta
    28801 ccgatgattc cgtcttcttc accaccgata gtaccaactt cagcttctac tgagatacct
    28861 ttagcgtgtg ctttttcaac aacttcttta gccaatttaa ggttttcttc aactggaagg
    28921 tgtgaaccgt caaacatgat tgaagtataa ccaacttcga tacactcaag tgcatcttcg
    28981 tagtgaccgt ggtcaaggtg gatagctact ggtacagtga tacccattga ttcaacaagg
    29041 ttagcgatca agttgcgagc aactttgtaa ccacccatgt atttagcagc acccattgaa
    29101 gtttggatca aaactggagc ttttttagct tctgctgcgc gcaagatagc ttgagtccac
    29161 tcaaggttgt ttgtgttaaa tccaccaact gcataaccgt tgtcacgggc tgcttggaca
    29221 aatttttctg ctgaaacgat tgccatttta tcaggcctcc tgtatatttt tatgggtcat
    29281 cccatttaca ttgttcattt tatcactttt tgccaaaaaa atctagtttt tcccgcagtt
    29341 tcaattgatt ttcttctaac tccacctatg taaacccttt ctctccctag tcttggacga
    29401 cttttggaaa atctataaag aaggttaaac gattctcctc catctcgaaa cgataagcta
    29461 atttttcatg ttctaataga ctcttaacca caaagagccc cataccagac cccttgacct
    29521 tgcgactagc attgtcagaa aaagactggg ctagtttttc ttgttcctct gagctacagc
    29581 tattttcgat aaaaagttct ccttctcttt ctccaattcg aactaagcca cctggaacag
    29641 agtgcttaat ggcattgctg atgagattag aaagaatcaa cttcataact gatgggttta
    29701 gataagcctg ctgatgggtc aaactattgt ctatctggag ctctctttcc ttggctagca
    29761 aggcataatc tttgaccaga ttttgcgtca tctggaggag gtcaattgtt tccctatcat
    29821 ctcgcaattc ctgcacagaa gagagggaaa gtatctgcag aacatggtga ttgagttcat
    29881 ccacaatccc caaggcaact cccagatact ggtctctatc cttataacga ccgatattct
    29941 ctctcatatt ttcgattagg attttcaaac tagccagcgg tgttttcaat tcatgagaag
    30001 ctcctcgtag gaattcgacc ttcatcttct ccagctggag aatggcttca ttcttttcat
    30061 gcaagtccgc aataacagtc aagagatgct ggtagaggct attgatttgt tccttgagat
    30121 tacctatctc atccttagaa tccacgcgca atcgcacttg ggaatccagg tccatcatcc
    30181 gacgggtcac ccgcttgatt tccaaaatcg gtgcaacaat agtccgagcg tagatgtagg
    30241 ccaccaagag ggaaatcaga aaggaggcca gcaaggtata gggaagaaac tggagactga
    30301 tttgctccgc ttccttttgt aaatccatgg aagctagaaa ctggagaatc atagtaccac
    30361 cgtcttgcgt tttcacctcg cgctcctcaa taaagagaga ggttgtctgg cggtctgtgt
    30421 ccagaggaag actgtccttg acttctaact tgtcctcggt catctcacct ttgacggtcc
    30481 ccttgatatc actagtctgg gaatacaagt ctaacacttg ctcgatactc tgcctatctt
    30541 tcccttctag ggactgggca atggctgttg ccttttgacc aatggtttcc tgacgatgac
    30601 tcagataagt cgaaggaaaa agaaaataaa tagctaaatg aaggcagata accagaacac
    30661 taaatatcga gaaggtatag ataaatatct ttgcaaataa acctgttcgt ttcattttcg
    30721 ctccaattta taaccaacat tgcgcacagt gaggatacaa tccaagtcta gctttttccg
    30781 caattccttg atataaacat caataacacg gtcaaaggga acctcatctg tcgctttcca
    30841 gacggcatcg ataatctgag accgagtcaa ggcccggcct tcatttttca ctagatagtc
    30901 cagaatttcc aactcttttg cattgatagg cacttcttga cctgcgaggc ttgcactgta
    30961 gctttcaaag tccaccttgg tatccttgta agaaaagatt cgtcctgtat cgtagtagcg
    31021 cttgaaaatc gcgtccaccc ttacttttaa gagggagaga gagaaaggtt tttccagata
    31081 gccatctgcc aaagaggcaa aggcactcat cttgtattcc tcatcctgaa aagctgtcaa
    31141 catcaagaca ggaacctgac tggttttacg aatctcagct aggacttcta agccgttaag
    31201 cttgggcatc tggatatcca gtaaaaccag ggccacctca tagctagaaa attgctccag
    31261 agcttcctga ccgtccgctg cctcaatagt ttcatagcca caatccgtca aataatcact
    31321 gaccccctca cggatcatct cttcatcttc tacaattaaa attttcatac tttaactgct
    31381 ctctattttt tatttttctt agaataaata cctactctat tttctattat agtctcttgc
    31441 tggccttttg tctgtaagca actgatcact agataaaacg ttgtgaaatt cctttctcat
    31501 aaattccata gctttagtat attatattta agcaataaag tacaaagaaa acaactgaaa
    31561 gcaatgattt tcaccactgc tttcggattt attttgaatt gttaaatagc cattcctatc
    31621 cactattctt gaatagaaac acaagatgca atctttattc cagactcatt ttttcaaatt
    31681 tattcaccat ccagcaagag ctcttttggt tgttttctaa ggagattgct tgaagcaagc
    31741 gccataacga gaaccactag aaccaaggca aggacaaaaa tgatgataaa gtctgatgtc
    31801 tgaatagaaa tgtctaggct cgacaaggtc ttgctaaagc catctacttc tgcaccacca
    31861 ccaaggttag aggcttgagc cgccttacta gcctgtttgg caacacctga agtcacattg
    31921 gcaaggacag tgtttccaat tgcacgggca gtgtaattag ctaggaagta agcagaaact
    31981 agagcaggga tagcaatcaa gatagattcg gtgatgaatt gacccaagat acttgcctgc
    32041 ttgaggccga tagagaggag aattcccact tccttgcgac gggcgttgat ccaaaggctg
    32101 agcaagaggg caaggaggag aactgagaag ctcaagctac cccagaagag gaggttggcc
    32161 atcttgtaca taccagagat agattgctca agagctgggt agttagagga gctcttgacg
    32221 agtgtgtagc tcttccagtt gataccactg atgccattca actctttcat aacatcatcc
    32281 aagttcttgt ctgctgttac aaagaaggtt gcgtccccat aaatggctgt gtcttctgtg
    32341 tatccataaa gttttgcagc agtgtgaatg tctgtaatag ctgtgttttc gtaaagttct
    32401 tgtgagtagg ttactgctga cttattatga ccatcaaaga gtcccttgat tgtcacttca
    32461 actgtttcct tggctccttt ttcattatct gcatcgtaga tattagagtc cagtttaacc
    32521 ttgtccccta ctttccagcc gtgtttggct gccaagtcct tgtgcaagag gattttatcc
    32581 ttgtcgtcgt tggttaagtg ctctccttcg actagtttat aagaaccaga gacaaacttg
    32641 tcttctttag aggagtcatt gacacctgta atcatcaagc tacttccaaa acgtttggca
    32701 cgatcagcag tgagattctt cttggtttct ggcgtttcaa tcaggtcata tccagtcaaa
    32761 tctccgatag cgttgatacg tttgacataa gactcaatgg ccttgttttc ggtgattttt
    32821 ttgatgtctt cacccttgat attcccagca ccgcgaggcg ttccttggtt gacgcgacga
    32881 ttgatttgca tggagaagct attggtgata tttttaaagg tctcctgaga agccttggca
    32941 gtagctccct tgattgacaa gccgaccaaa ctcaagctcg ccatgaggag aataatcagg
    33001 aagatgacaa tcgatttgaa aaacttcctt gtaacatagg caaatgcgtt gtgtaacata
    33061 gattcccttt ctagattttg ttttaatcat tctattaaag taagctcaaa ttatttacta
    33121 gtattgcgcg tttcagtcag tttcttatcc tttaattcaa gtgtaatatc tgacgcttgt
    33181 gccacttctt tactgtgagt tacgacaatc acacatttac ctgttttctg ggcaagtgat
    33241 ttgagtagtt cgacaatatc tccagcagtt ttaggatcca gatttcctgt tggctcatca
    33301 gctagaataa ctggagcttc tgagaccaaa ctgcgagcaa tggcaacacg ttgctgttga
    33361 ccacctgata actggagaac attccgcttg atctggcttt catccaaacc aagctcaaga
    33421 agtgtattct tgcttgcctt tttgttgacc aatcggatat tttccagcgg agaaagataa
    33481 tctatcaagt tataattttg aaagaccagg gaaatatggt gcatgcgatg gtaagaatag
    33541 cccttcttac gaatatcctc tccttgaaaa aggatagaac cttcaacagg actatctaga
    33601 ccagcaagta gggacaagag tgtggatttt cctgctcctg actccccaat aatactgtaa
    33661 aattttccgg gttcaaaatt ataatcgatc tgatatagga ctgcttcagc agtattctta
    33721 taacggtagg taacatcttg taattgtaat aaagtcatga tttctccttc ttaactaata
    33781 gatgataaaa tttctttcgg tgattttcta aataagaata ggaaacaaag ggctacagat
    33841 aagcaactaa gcagaactag aaaaacatag gattctgcaa aagataagat gctagttgat
    33901 aaactgcttg ctttggctag tgtatcttgt aagcttgcct gatctccact tgctagtaga
    33961 gtttggagta ggtaagttgt gattccgttt cctgcaacaa atgctggaag caaagctcca
    34021 agagatacca aaactacctc taaacagaat tgtaggaaga tcgagctctt gccttttcca
    34081 agtgcaagta aaatccccac ttcatagacc cgttctctca accagagaga caaaaccaga
    34141 attaaggctc cagctcctgc tatcaacatc ccataaagga agatggtcag gaaggtttgg
    34201 aaagttgcaa ctgagtcttt gatttgttca aaagccttgt tttccttttc gacttggtag
    34261 ccttgatttt ccaaggccaa gttttctacc tgcttcatga gtccgtccat ttccttagga
    34321 ttttctacat agaagcgtgc tgcactgact tgagcttcac tattgcccaa aagggtttgg
    34381 ctactttcat agtctgtaaa gacttgattt tcactgaagt cagaagacaa gcctgtgaat
    34441 ttctcttgtt ttttaccaga aaagatgcca ataatctcaa actctactgt ttgtcctttt
    34501 ccagattcag actgaccagc atccaagcca atcttgtcat gaagtgaaag accgttcttc
    34561 ttagccaatt cttcgtggat aaggattttc ttggaatccc ctttttgaag gtgtcgccct
    34621 tcttttagat tgaaagccga actggtaaag gttacatcct tggatgaatc ctcaagagcc
    34681 gttaagctaa ccaagttatt gtctgcagct gataaatcat cacgctccac gctctgctcg
    34741 ccagtcactg cttccttgtc ttttagtttt gcgaccgtct caagttcagg agagacattt
    34801 tccagcccct taatcttgct tacagatgct aggtctgaca acttgaatgt ctgaccattc
    34861 tctatcttct taatagaaaa agatgtattg agtgatttat aaagattgct ttctactgtt
    34921 ttgttggact tcatcagagt caaacaggct gaaattccgg ccaataagac caataaaatc
    34981 agaaataaaa taaaacttct cagtcgcttt ctgctgacat aagcccaaga tctttggatt
    35041 ggattcattt gtcacctcca tatttgtaag actattataa aacccaaata tgaaatgttt
    35101 atgaaatacg aaaaaaaaat atcgagtagg ggataatctc tagcccctct cacaccaccg
    35161 tacgtgccgt tcggcatacg gcggttcaac taacttttaa cgcatgtcgt tcaaggtaat
    35221 aatccaaaca cgaaacctgt tcacgttttt caaggactgg ttttgatata gcacgtttaa
    35281 gtaccgactt ctgagctact atagtagatt gaaactagaa tagtacatct cttcttctaa
    35341 aacattgtta gaaatcgatt tggctgccct gatcgattcg tcctactctt atttcatttt
    35401 actataattg atagtggtcg ctccagccag ataccttatc tgctatccat ttaggaaccc
    35461 ctaacttaag caatccccat aatcgtctcg atttcttctt ccattgcttc cagataatca
    35521 ctcgtaggcg agtacgcaag cgctcatcta tgctagtgac tatacttttc atatttataa
    35581 ttcattcctt tcgtttcact caaggcacaa cacagaatga aaaagtgttg tgctctttat
    35641 tttgttttat aataatagtg agaaagccta tcactactac aaatcacggg gaggtgaata
    35701 agtgagtggt acagccacta cctcgcatat tttgtcacat catttaacgg tacataataa
    35761 gttgtaccat ctgaataagt tgctacaata tcatttgcat gctctccttc acctttagca
    35821 aaggttggag ctcctgctgg ataattttta tttgcctctt tcaatttttc aataatggca
    35881 tttttttctg tatcttttat attatcagga tttttcacta agattttgtc tggatatgtc
    35941 ggtttagcag aaacaatttt tactgttatt tcttttttat tcgaagcact tgtccagttt
    36001 ccagcattat ctttagcatt taattttaca gtaattcctg aactaggaac ttcagtagca
    36061 ggttgattat caacattatt caactttaat ttcaaaagag ctgttgcatc agacgtttta
    36121 tcaatcgttg tatataatga tgaattgtta ttataaccag tttcttcata tttagctgtt
    36181 tgtgagctac ttgaaacaga actgaaatta tgcccactac ctccctgatt atcttcaatg
    36241 cttacgtcta aatgaacttc cccactatta tttggcttag caacaactgt tatagtaaaa
    36301 taacataaaa tttgcataaa tagattaggg aaatcaaagc agcttctagg aatgttttag
    36361 cagtcacagt gtactttccc agcatcaagc cactataact ctgcacataa aaatggagaa
    36421 gatggcaatc ctcttctcca aatattaact tctttacaaa ccaactatag ttgacaaaga
    36481 acctaaaatc aattgataac acaaggtcag gtcggtcaac tctttcaact gaagccctgt
    36541 caactcttcc catttatcaa tcttgtattg gagagaattg cggtgcagat agagttgctg
    36601 ggctgtttta gtgagaacag cactattttc ccaaagagag agaatgattt cctgaatctg
    36661 atcttgatcc aaaatcatct ggtgtagaca ttctttaatt ggcttcaagt ccacgagtct
    36721 ttctcccata ctccaaagat agagctgaga aaaagtatga acaccttggt gaccctgacg
    36781 ccaccatgtc ttgaacaaat cccgctcagc tttgattaag tctgataggg cttgatgtcc
    36841 tgtctgagac caaacctgac ccaacatgat agaaagacga agtccaaagt catactcaac
    36901 cgcttcaatc gtatcactta aaatatctct tacagaagtg tatttgtctt gttgaagcac
    36961 gaaaacataa tcctgagctc cgacctgtag cactgtctga caattcggaa aaagagtccg
    37021 catcatatct agccaagaag ccagattttc ctgctgaaaa taagaaagat ggcaataaac
    37081 caactgaatc tttttaaaaa cttgcggtgc ctgtcccttg ccttcaacca gataggaata
    37141 ccaagggttt agcgaacgag cctgctcctg ctgggtcaaa agggcaacca actgcttttc
    37201 acgctcgctg agcccagctt cctccagcaa aatccactgc tgagaagcta aagggagcgt
    37261 gagatagccc tctttctcta ctggttggtc tgaaatccga gcctcaggaa accagtcttg
    37321 tagttctttt tccctcatgt tctagccctc cactttttgg atgcaccatg aaaccaaact
    37381 ctcaagacgt tccagattct cagtcatata gagatagccc ataaccgctt caaatcccgt
    37441 ggacatacga taagtcacga catctgcatt tttggccttt gtgtggctat tggtattgcg
    37501 gccacgtttg tagatttctt cttctttttc cgttaggacc tgctcctcca acatgagagc
    37561 aatcaggcga gcctgagcct tggctgacac atacttggtt gcttcttgat ggagtttatt
    37621 gggtttggtc atacctttga ggatgaggtg acggcgaata tacatagaat acaccgcatc
    37681 cccctcaaag gctagcgcaa tcccgttaat gagattgaca tcaatcacgt gtccacctca
    37741 ctccatcctt ggtatcaagg agcttaattc cttgagtaac caattggtca cggatttggt
    37801 ctgctgtcgc aaagtcacga ttggcacgcg cctcttggcg tttttgaatc aagtcttcaa
    37861 tctctgcatc caaaacttcc tcaacaaaga caattccaaa aatttctaac atatctgcaa
    37921 gagcttgctt gacacttgca tcatagttcc ctgagttgat ccatttggcc atttcaaaga
    37981 caactgtgat accgttggca gcattaaaat cttcatccat agctgctaca aacttatctt
    38041 taaagttttg taactcttgg gcatccacgt ttcctgtaaa tggttgttcg taagtattct
    38101 tcagatactt gagattggtc tcggcatcgc gaactgcctt ttccgtgaag ttgataggct
    38161 tacggtagtg ctgggtcgca aagaagaaac gaagtacttg cccatcaaga gttttaaggg
    38221 catcgtgtac cgtaatgaag ttacccaagg acttagacat tttgacattg tcgatattga
    38281 caaagccatt gtgcatccag tagttagcaa aagccttgcc tgttttagct tcagattggg
    38341 caatttcatt ggtgtggtgt ggaaactcta ggtcagctcc accaccgtgg atatcaatgg
    38401 tatcacctaa aatctctgtc gacatgactg aacactcaat atgccaaccc ggacgtccag
    38461 gtccccaagg actatcccaa gaaatctcac ctggtttgga agatttccat agagcaaagt
    38521 ctacaggatt ttccttacga gccgtttctt catcggtacg acctgaagca cctagctcca
    38581 aatcttccaa ggttttatta gccaatttag catagttgtg ggatttttct acacggaaat
    38641 agacatcccc ttgactctca taggcaaagc ctttctcgat caagtcttcc acaaaacgga
    38701 tgatgtctgc cataaactcc actacacgcg gatggcgagt cgcaggtttc acgcccaagg
    38761 ccgtcacatc ctcacgaaag gcagcgatgt acttatccgc aacctcctga ggcgtgatgc
    38821 cttcttccct ggcacggttg ataatcttat catccacatc tgtaaaattg gaaatatagg
    38881 caaccttata cccacggtac tcaaaatagc gacgaatcgt atcaaaagct accgtcgaac
    38941 gggcgtttcc cacgtggata tagttgtaca ccgttggccc acaaacatac atcttgatct
    39001 tgccgtcctc aatcgggaca aattctcgca aatcacgaga catggtgtca tagattttaa
    39061 tcataaatca taatcaggaa agctaaaatc caagaactgt tagtttcatc actaaactaa
    39121 gagctcaagt aaatttcagt ccgaatgtct ctacacttcg gaatcccttg ctcctttctc
    39181 attcagataa accacctgtg tctgtttgac aaagccaatt ttttcataca aacgtttggc
    39241 acctacattg ctatcttcca ctgcaatctg aaattcctta tcattttgct caattagttg
    39301 gttgacaagg gattttgcta agtagcttcc atagcctttc ccacgttcag gttctgatat
    39361 tgctaaaccg tagaagtaat tcgtatcgct cgataaatca accgtgcaag tcccaataac
    39421 ctgaccagct tttaataaaa tatatagtcg gctttctgga tctttcagag cttcagcgac
    39481 atatctatcc acaacttctc tcgattcatg ttcctctgaa aatgcctgaa attttaattg
    39541 actaatttga tcctgatacg aactatctgc taacaaaatt tcaagatggg aaacatttgc
    39601 taacggataa ggtcttctat ccttacctaa ccaagtttct gtctcttcat cctcgattag
    39661 tccccagtta ctggcaaagt caggatgatt ctctaaaaaa atacgttctg tctgaaaagt
    39721 gactgaccga atggggaaag aagctgtttc tctctcaaaa ctagtaaaca atgcacgcgc
    39781 aatcccctga cggcgatgac ctggatgaac cagtatcgtt acttctacat cttggtcatc
    39841 tgcatagaca gttaataaac caacaagttc gcctttttca taataaagga aaaaggcggg
    39901 catgtttggg tcaaaattaa gcatgttaga gagataggga tcgcgatagg taccgtcata
    39961 gttttggcaa cagttaatta cttttttcgc ctcagatagc tcctcttggc ttaacttgtt
    40021 tcttgcttga atcatatagg tatcctctac aaaccagacg atctgtgact ggcatcttta
    40081 gcctgctcga gtttattgac ataatactct cgtttttctt cgacttcgtg aatgacaggc
    40141 tcatctttct taccatgaag acggacaatc ttggccggaa taccgacaac cgtcacgtca
    40201 ctaggtacat ctgctacgac aactgctgca gcaccgacct tggcattttc accaatttcc
    40261 acaggcccga taacttgggc atgggctgat atgagggctc cctttcgtac agtcggatgg
    40321 cgtttgccac agtctttccc tgttcccccg agagtcactc cgtgatagag aagaacgcct
    40381 ttttcaacaa tcgctgtctc tccaatcacc agaccagaac catggtcaat aaaaacacct
    40441 gaatcaatct gggctcctgg atgaatctca atctgagtcc aaaagcgcca aaactgactg
    40501 tacatacgag ctaatagttt gaagccatgc ttccagagaa aatgcgagag acggtgggcc
    40561 gccaaggcct tgacacctgg ataagtcagc aaaacctcca aagtggtgcg ggccgctgga
    40621 tcattttctt ttacgatatc aatggtttcg cgccaccacc ccatacattt ctccttttct
    40681 tattctgaat cttttggtgt ttctgtaaat tctttcttag gtttgtgatc cttgtgatga
    40741 cgtgggcggt gagggcgctc agacttttca cctttttcat catgctcagg ttttggcgga
    40801 cgaggtagaa gagccttcat agaggcatcg atacggcctt tttcatcaat cttgataacc
    40861 ttaacatcaa cttcatcccc gatttctacc aaatcctcta cacgattggt acgagtccaa
    40921 gccatctcag agatatgaac aagggcatct gtcttatcaa agaggttaac aaaggcacca
    40981 aatttctcga tacgaacgac tttagcacgg taaacttcat ccactttggc ttcacgaacc
    41041 aaaccagcaa taatttcttt ggcacggtta atagcatctt ggtcactaga gtagatagac
    41101 acatttcctt cttcgtctat atcaatctta acacctgttt cagcgataat cttgtcgatg
    41161 gtttctccac ccttaccgat gacaatctta atcttgtcca catcaatctt gatcgtatca
    41221 attttcggag cagttggagc caattctgga cgaacttctg gaatggttgc ttcaatgaca
    41281 tcaaggattt caaaacgcgc tttcttggct tgagcaagag cctccgtcaa gatttctgca
    41341 gtaatccctt gaatcttgat atccatttga agggctgtaa tcccatcacg agtacctgca
    41401 accttgaagt ccatatctcc aaagtgatct tccaaacctt ggatatctgt caatactgtg
    41461 tagttatttc catctgagat aagtcccata gcaataccag ctactggcgc cttgattggc
    41521 acaccaccag ccataagggc aagagttccc gcacagatag aagcttgaga tgaagaaccg
    41581 tttgattcca aaacttctgc tactagacgg atagcgtatg ggaattcttc caagcttggc
    41641 aagacttgag caagagcacg ctcaccaagg gcaccgtgac cgatttcacg acgacctggc
    41701 gcaccgtaac gacctgtttc ccctacagaa tattgaggga agttatagtg gtgcataaag
    41761 cgtttcttgt actctggatc caaaccatca atgatttgag tttctcccat cggagccaag
    41821 gtcaagactg aaagagcttg agtttgccca cgagtaaaga gacctgaacc atgtacacga
    41881 ggaaggaagt cgacaaccgc atccaaagga cggatttcat cgaccttacg accatcagga
    41941 cgcaccttgt cttctgtaat taaacgtcgc acttctgcgt gttccatttg ttccaagatt
    42001 tcagccacat cacgcataat acggtcaaat tcttcgtggt ccgcatattt ttcttcgtaa
    42061 acggcagtca cttggtcttt cactacttga gtcgcagctt cacgggtcaa tttctcttct
    42121 acttgaactg ccttttggag gtcactgttg taggctgcaa tgatttcagc ttgcaattca
    42181 gcatccacgt gaagcaattc cacttctgct ttttctttac cgacagcagc aacgatttct
    42241 tcttggaagg caatcaattc tttgacagct tcgtgccctt taaggagcgc ttccaacatg
    42301 atttcttctg acaattcttt ggcaccagac tctaccatgt tgatagcgtg cttggttcca
    42361 gctactgtca attcaagaag agattgctct gcttgttctt gacttgggtt gatgatgatt
    42421 tggccatcta catatcccac ttgtacccca gcaattggtc cgtcaaatgg aatatctgaa
    42481 atagacagtg ccaaagatga accaaacata gcagccattg gtgcagatgc attttcatca
    42541 taagaaagca ctgtattgat gacttgaact tcattacgga aaccttccgc aaacatagga
    42601 cgaatcggac ggtcaatcaa acgcgctgtc aaggtcgcat ctgttgaagg acgtccttca
    42661 cgtttcataa agccaccagg aaacttccca gccgcataca ttttttcttc gtagttgact
    42721 tggagtggga agaaatcccc agttgccatt ttcttagaca taacggcagc agtcaagaca
    42781 gttgactcac cgtaacgtac gacaacagat ccatttgctt gcttagcaac ctgaccagtc
    42841 tctacaatta actcacgacc cgcaaaagtc gtttgaaaca cttgttttgc cattttaatc
    42901 ccctttggat tgatgaaatt atacgccttg cctacaaaga tcaagatacc aaggacgtca
    42961 aaagcaaagt aaaaatagga aactgacgaa gtcttcgatg aagacaagac agtttatctt
    43021 ttttacacag cttttcggcc gtgttcaatt acacaagata ttttggacgg ttcggcttgc
    43081 cgaacatttc tgtagaaaaa taggaaggtg acgtcgcact cgacgagtgc taggaagctt
    43141 atcttttttc ctaagaaatg agaccaaaat tcaagtcatc aagataccaa gccgtcaagc
    43201 aactcaaagg aagataggaa atcgaacgac ggagcgactg ctcctaggga gatttatctt
    43261 tttccacaga gttgtaggca agttcagttt tcaagataca tcattagaaa ggtttaatac
    43321 taaagtatct aaagctttca cgctaatcgc tatcgggcga ttagctaaat gctttactaa
    43381 ctctctcgtc aaataacatc gatttgactc actcgtgtcg ttaaatctta cagtttaaat
    43441 gcattgtatt atttaatacc ttcatctttg tatcaagtac gtacagaatt tattttatca
    43501 tatttttctt aaaaagtgag gtctttacca ttaaaaagga accattcccc tcacctgaga
    43561 agaatggttt gcttttatta tcctagagac tggtgattaa acaaggcatg ggttgcttga
    43621 tggatgtatt ttgctgtatc agcattattc atcgtataga gatgcacacc ggcaacatcc
    43681 tgagttacca agtccacgat ttggtccact gcataggcaa gtcctgctgc tctgagcgac
    43741 tcagggtcat gctcatactt gtctaagatg gctttaaatt tgcgtggaag atggatattc
    43801 tcacaagtct tcaagagtcg gagagcctga tttcgattca gaattggcat aattcctgca
    43861 tgaatgggaa catcaatccc agccaagata cacttgtcct gaaaatcata gaagcgctca
    43921 ttgtcaaaga agagctgagt tacgaggctc gaacagcctg catccacttt cttcttaaga
    43981 ttttgaatat ctgaaatctg atttggcgaa tctggatgcc cttctggata gcaagctcca
    44041 ataatatcaa agtgaggggc ttgttccttg ataaactcaa tcaagtcggt tgcatagcgg
    44101 aaatcctttt gtggttctac gtctggaata atatccccac gaagagccaa gattttctga
    44161 accccaactt tgtccaagtc aacaatagtt tcagcaacct tgtccttagt tagataaata
    44221 gctggcaagt gggcaatggt cggaatcgcc aagtcatttt ggataaagtc agccaaacga
    44281 accgtcgttt ccttgatatt aaatttatta ttgctggcag ttacactgat aaaatgggga
    44341 gccaactcct gcatatcctg caaggctgaa atactgttac cattacccac ggctgggttt
    44401 ggagggaaca cttcaaatga gagtgacggt gtttggcgtg acatatgtaa taaccttttc
    44461 tagttgattt ctttttgaac aaccactgta tggagagaaa tccaatctta caatttctca
    44521 cgcgcagctt tagctgcttc aacaaggcgg atcaagcttt cttttgtttc tgggatacca
    44581 cgtgttttca aaccacagtc agggttgatc caaactttct tgcttggcac tttagcaagg
    44641 atggcttcga ttgtgttgtc gatttcgcct tcatttggta cacgaggtga gtggatatcg
    44701 taaaccccag gtcccacttc tgtttggaag tttttcgctt tgagttcgtc caagatttca
    44761 aggtttgaac ggctagcttc aaaggaaata acgtctgcat ccatgttatc gatagctggg
    44821 atgatatctg taaattctga gtaacacatg tgagtgtgga tttgtgtgtc tggcgctact
    44881 gttgagtgta ccaagcggaa ggcaggaatt gcccagtcaa ggtagtcttc gtaccagtcg
    44941 ctacgacgga gtggcaattt ttcacgaaga gcagcctcat cgatttggat gatcttcaca
    45001 ccagcaactt caaggtcaag tacttcatcc ttgatagcaa gggcgatttg aagagttgaa
    45061 tccttgatag agatgtcttc acgtgggaat gaccagttaa gaatggtaac aggtccagtc
    45121 aacatacctt taacaggttt gtttgtacga ctttgtgcat agctagacca tttaacagtg
    45181 atagggttaa gacgagtgac atcaccccag atgattggtg gttttacccc acgcataccg
    45241 tatgattgta cccatccatt tttagagaag aggtatcctg acaagttttg accgaagtac
    45301 tcaaccatgt cattacgctc aaattcaccg tgaacaagga catcaaagtc aatatcttct
    45361 tgccacttga tccattcgtc aatcgtttca gcaaggaaag catcgtactc tttttgagac
    45421 aattcacctt tacggtaagc caaacgtttg gcacgaactt cttttgtttg agggaatgaa
    45481 ccaatcgttg ttgttggaag agctggaagt ttgaaagctt cttcttggat agcttcacgt
    45541 tctgcaaagg ctggcaaacg agtgtagtct gcgtctgtca agtcagcgat acgcgcacga
    45601 agttcagcat tttcaccaac acgctcagtc gcaaagagtt ctttgttggc tgcaagagct
    45661 tctgaacctt gaccattgcg gatagcatcc aagtcacgga tttcatccaa tttttcaact
    45721 gcaaaggcaa agtggttcaa gagtgctggt tcaaattctt cattagcagt tgtaaatggc
    45781 acatgaagaa gtgagcaaga gcttgtcaaa acgatgtttt cagctgggat ttgctcaaga
    45841 acagccaagc tcttttcgta gttgttgcgc cagatgtttt taccattgac aatacctgca
    45901 tagagagtct tgtcagctgg gaagccacct ttaacgagtt caagagtttt cttaccttca
    45961 acaaagtcaa gaccgatagc atctactggc aagtttacaa ggtcagcgta tacgtcacga
    46021 acatcaccga aataagtttg aaacaagact tcaagacctt ttttgtcagc caagagtttg
    46081 ttgtaaaggt tcaagaagag agctttttct tcagctgtca agtcttttac aagagccgct
    46141 tcatccaatt ggatgcgagt cgcaccaagg tcagccaatt tagcaaaaac ttcttggtaa
    46201 gcagccacaa agctatctac gaagtcgtct gctttcacgc cttcttcaaa gtctgacaat
    46261 tgaaggaaag tgaagggacc tacaagaaca ggacgagtgt tcaatccaag ttctttggct
    46321 tcttgaaact catcaaaaat cttgtgacct gcaagtttga cttgagtgtc tttttcaaat
    46381 ttaggaacga tgtagtggta gttagtatta aaccatttct tcattgggag ggcgcgaacg
    46441 tccccttttt ctccttggta accacgtccc aaagcgaagt agcgctcaag gtcagacaag
    46501 ttcaagtttt gaacggatgc aggtactacg ttgaaaagga aagctgcatc taggaagtta
    46561 tcatagtgag aaaagtcatt tgatggaatt tcagtgatgc ctttttcttt gacaatgttc
    46621 cagtgtttag cacgcaagtc ttttgctgct gctaaaagtt cttcttctga gatttctttt
    46681 ctaaagtatt tttcagttgt aaattttaat tcacggaatt cgcccaaacg agggaaaccg
    46741 atgattgtag ttgacatgat gtgtcctcca aaatttgttg ttgaaactat cttaacagaa
    46801 aagaaagcgt ctgtataatt gtaaaaaatt agggtttgat atagtttgaa actatatatc
    46861 tgtttcggac aaaagaaaaa gacttgaagc aaacgtctca aatcctttgt aattcttact
    46921 ttacagctat attccaatta gaatactaaa acatgttatt agtaattctt ataagtgact
    46981 atgacctgtt attagaaaag actataactg attctagtca acttttcccc tgttcaagtg
    47041 gaacgactgc tagtgtcttt cctagactgg ttagaacttt taagaccgta tccaactgaa
    47101 gactgctctc tcctgtttcc atcctagcta tgacaggttg actcactcca ctgagttctt
    47161 ctagctttct ctgattgatt ccttgttcat gtctagcttc aatcaactcg ctcataatag
    47221 ccactcgcat atcactttca aggatttcct ccttgctaaa gagctcagat ggacatcctt
    47281 ccaattactc ccaatagcac tattcttcat cacttaaccc tctttctttt acgtctatgt
    47341 atttttgaaa aaatgagcga attatgattc gatagactga ccagcaggtt taaaattggg
    47401 actagcttgt ttcttaagcg cttttccttt tctaggataa agcaaatcct gtttgcttaa
    47461 ccccaatttt ccatgatgaa tccaatagta aatggttgaa attcccacgt taaccccttt
    47521 agccatcacc atcatttcag gcgaaaattt ttggttatga tagtggagaa tcttttcctt
    47581 tagttccttt gtcaagcttg atttcttgac cgagtgcttg cgattatttt cataagactg
    47641 ttgagcatag tcggcagaat aaacctcttt gaagcgccct tttccaagac attttcggac
    47701 tgtcccatac ttgatttcag tatggatagt ttgaggagct tttccaagta gagaggcaat
    47761 ttctctattt gattttcctt cttttttcca tcgttcgatt aagcgacggc tatcgattgt
    47821 caaatgtttg ccttttgtag tataattgtc ttgcatttct gtgcctttta atcatttcaa
    47881 tcttaaattg gacttttttt acttgggttg tacttaatct atgaggaaga caagaaaaag
    47941 aatatcaatc aagtaaagtc acaaagtcac attagctccg agcaaccatt gcaaattgag
    48001 gtactcacac aatgattaaa acatttctct ctgccctttc ggtcattctc ttttctatcc
    48061 ctatcataac ttattctttt ttcccatctt ctaatcttaa catttggcta tctacccaac
    48121 ctatcttggc acagatttat gccttcccct tagctactgc aactatggct gctattttaa
    48181 gtttcttatt ttttttccta tctttttaca agaaaaataa acaaatacgg ttttactctg
    48241 gcattttgct cttactatcg ctcatattac tattattcgg aacagataaa accctttctt
    48301 ctgcatcaaa taagactaaa accttaaaat tagtaacttg gaacgtcgct aatcaaatag
    48361 aagcacaaca tattgagcga atttttagcc attttgacgc cgatatggct atattccctg
    48421 aactagctac caatatcaga ggtgagcaag aaaaccagag aatcaaacta ttgtttcatc
    48481 aagttggact ttctatggcc aactatgata ttttcacttc tccacctacc aatagtggaa
    48541 tagctcctgt gactgtgatt gtcaagaaaa gttatggttt ctatacagaa gctaaaactt
    48601 ttcatacaac acggttcggg acaattgtat tacattcgag aaaacaaaat ataccagata
    48661 tcattgcctt gcatactgct cctcctctgc caggtttaat ggaaatctgg aagcaagact
    48721 taaacatcat tcataatcaa ttggcttcaa aatatccaaa ggctattatt gcaggtgatt
    48781 ttaatgcaac tatgcgtcat ggagcacttg caaaaataag ctctcatagg gacgcattaa
    48841 atgtactgcc accttttgaa agaggaactt ggaatagcca aagtccaaaa ctttttaatg
    48901 caacaataga tcatatttta ttgcctaaaa accactacta tgttaaagat ttagacattg
    48961 taagttttca aaactctgat catagatgta tttttacaga aatcacattt taattatttt
    49021 atataaaatc acccctctag tgttcataaa ctagaggggg aatttgtatc ctactatcgt
    49081 ttaacgcact tctgcattca ctttttcttc gagcgatgct tggatttttt ccatatagcg
    49141 tgcgacttct tcgtccgtta agctatcttc tggattttgg aaggtcaagc tataagccat
    49201 tgacttcata ccaagtccca atttctcacc tgagaagacg tcaaagagtt tgatatctgt
    49261 caaacgtttc acgccggcag cttggatagc atctacaact tcttgatgag tcacttctgc
    49321 cttgaggaga agggcaacgt cacggctgac tgccgggaat ttggtgattt ctacaaatgg
    49381 agtcgctggc tgaagcgcag cttcgatagc tgaaaggtta agctcagcta catacgtttc
    49441 tggaatatca taagccttag cagtgactgg atgcacttgg ccaaggaaac caagaacttg
    49501 gtcaccgagt gaaatcacgg ctgttcgacc tggatgaagg ctagcaattt cagctgttgc
    49561 tgtataagtc actgcaagtc ccaagcgagt aaagagcgct tctaggattc ctttagcata
    49621 gaagaaatca actggaactg ctactgtttg gaaatctttt tcagcaacca agcctgtcaa
    49681 ggcaaaggca aaactgttga tttcatttgg aagttcttct tttggattac ctgtttgttc
    49741 aaagactttt ccaatctcat aaagggccaa gtttttattc ttacgagcca cgttgtaggc
    49801 aacggtatca aggatccctg aaatcatatt ttgacggagg actgaacgat ccacagtcat
    49861 tggccacatg agttccgtaa ggttacttgg ttgagctgtg aactcaactg ctttttcagg
    49921 agttgtcaga gcataggtga tgatttctgt caaacctgct ccttcagcaa tggtacgaac
    49981 ttgacggcgg agtttttgtg tcacagtcaa ttcaccagct gtaccatcgt cttttggaag
    50041 gctggttggc aagcggtcat atccatagat acgagcgatt tcttcaaaga ggtcagcttc
    50101 gattgtgata tcccaacgac gacgtggtac gctgactgta aagctgtctg catttccaga
    50161 aagaccaaag ccaagacgac ggaagacgtc ttcgacatca gcataagaca gctcagttcc
    50221 gaggacacgg ttaacatcag caagggttga agaaacttct acatctgaag tatcaagctc
    50281 acccgctgaa acgataccct tacgcaccgt cgcacctgca agttccgcaa tcaggctagc
    50341 tgccgcatca agggcttcat taactgttgc cacattaatt cctttttcaa agcgagaaga
    50401 tgactcagaa cgaaggttca ggcgaccact tgtcttacgg atagttttgc cattgaaaac
    50461 agcagcttca aggacaacac gactagattt ttcagagatt tctgttgctt gaccacccat
    50521 gacacctgca agggctactg gcttgtctgc aacagtgatg actaggtcat tcacgtccaa
    50581 gtcacgttct tcaccgtcca aggtcaccaa tttttcacca gcacgcgctt cacgcacacg
    50641 gatgtcagtc ccttcaaatg tgtccaagtc aaaagcatgc ataggttgac caaagtagag
    50701 caggatgtag tttgtcacgt ctacaacgtt attgatggga cggatgcctt cgttcatgag
    50761 aaggttttgc aaccattgtg gacttggtgc gatagtcaca ttgtccaaga tacgagctgc
    50821 atagtaaggc gccttgtctg tcccaatgct gacagaaagg gcatctgccg cagcttcatt
    50881 agtttctgtt agagtaaatt ctttaaagtt gactgccttg tcatagatgg ctgccacttc
    50941 gtgagccact ccacacatag aaagggcatc tgcacggttt ggtgtgatgg aaagttcgat
    51001 gatttcatca tccaagtcta ggtaagaaaa gacttcctca cctggcacgg cattttctgg
    51061 caagatttgg atgccatctg cgaattcctt aggcacaact gagtcagaaa ttcccaattc
    51121 accaagtgaa cagatcattc caagtgactc caaaccacgg atttttcctt ttttgatttt
    51181 gtagttatca gcgatacgag ctcctggaag agccaccatg accttgatcc cagcacgcac
    51241 atttggggca ccacaaacga tctgacgctc ttcttcttcg ccaacgttaa tctgacaaac
    51301 atggaggtga gtctctggca catcttcgca agacaagacc tcaccgacga caatttttga
    51361 gagaccagca gctggtgatt cgacaccctc tacctcgatc cctgtagttg acattttttc
    51421 agccaactct tgtgatggca catcaatgtc caccaattct tttaaccatt tataagatac
    51481 aagcataatt tagttctcca gaatgaccat tgccacactg gttcttttcc tttcctatca
    51541 tttcaataga agaattctct tcttacctta atttctttct cagtaaccaa tccgtatcta
    51601 ctttttgacc aaccataaaa tgatgttggc taaatttttc aaaaccatat cggttataaa
    51661 acgcttgagc ttttgtatta tgctcccaaa cacctagcca agcccaagaa aaactatttt
    51721 ttgtagcaag ttcaagtgcg aattcaaaca gttgcttacc tagtccaaat ccttggaatt
    51781 tttgtagcac atagaggcgt tgaatttcaa aagcgtcctc taattctctc tcagtttgag
    51841 cacttcccca gttgactttg agaaaaccag ctatctcctc ctcatgcata atgaaatagg
    51901 tttcagagtc aggatttccc aactcagttg acaaagtttt cagactataa gcctcttcaa
    51961 agtattcctg taactgctct tccgtattat catacgcaaa ggtttcacga aaggcttgtt
    52021 tggcaatttt agccaacacc tcaacatctg ccatttctac ttttctaatc attatttaaa
    52081 ctgttctgag aagcggacat ctccttggta gaatccacgg atatcgttga ttccataacg
    52141 gagcatagct acacgctctt gtccaagacc aaaggcaaag ccagagtata cagtcgcatc
    52201 gataccactc atttcaagga cacgtgggtg aaccataccg gcccccataa tttcgatcca
    52261 acctgttttc ttacatacat tacagccttc tccaccacac ttgaagcaag aaacatccac
    52321 ctcaacagat ggctctgtga atgggaagta agatggacgc aaacgaattt gacgctcttc
    52381 accaaacatt ttttggacaa tcaactgaag cgttccttga agatcagcca tagagatatt
    52441 tttcccaact accaagcctt cgatttggtg gaattggtga ctgtgggtcg catcgtccgt
    52501 atcgcgacgg aagacacgcc ctggcgagat catcttcaaa ggacctttag aaaaatcatg
    52561 ggcatccata gcacgcgcct gaactggaga cgtgtgggta cggagcaaga tttcttcagt
    52621 gatatagaaa gtatcctgca tatcacgagc tgggtggtct tttggaaggt tcatacgttc
    52681 aaagttatag tagtcttgct ccacttcaaa accatccacg acttgataac ccataccgat
    52741 gaagatatct tcgatttctt cactggtttg tgtcaaaacg tgacggtgac cagtcgcaac
    52801 tggacgacct ggaagcgtca catcgatact ctcgctagcc agttgagccg cgactttctt
    52861 ttcttccaag agcttagctg tttcttcaaa agcagctgtc aagacatcac gagcttcatt
    52921 gacgtgtttc ccgatgattg gacgcatctc agcagaaaca tctttcatcc ctttgaggat
    52981 ttcagtgagc gaaccctttt taccaaggac agagacacgc aaatcttgca tctctttttc
    53041 atttccagca gtaatctgct tcaagctagt cagcgtttct tcgcgaagcg cttttaattg
    53101 ttcttcaata gttgacatat ttcctccatc agtctctcgt agataaaaag aaaaccacat
    53161 gccaaaaact ccactcggag cgttgacacg cggtaccatc cgttttcatc tgacaagtca
    53221 gaccttcatt tctaaatcca tgcgcaagtg aattcaccca gctttcatat agagaacttg
    53281 cagtcacggc tctcctccct gatatacttc ccttgggcta ctagtctttc agattcctat
    53341 tcaattacta cttagtttat cagattttta ccattcttgc aagacctatc ttacttctgc
    53401 ttgttagctt attcttatct aactttatag cggattgaaa ta
//
LOCUS       DAVGJJ010000004        51100 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000004 DAVGJJ010000000
VERSION     DAVGJJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.004"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(197..655)
                     /locus_tag="VVE35_000189"
     CDS             complement(197..655)
                     /locus_tag="VVE35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU3060449.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWSAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(652..1092)
                     /locus_tag="VVE35_000190"
     CDS             complement(652..1092)
                     /locus_tag="VVE35_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3060450.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIELAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1448..3397
                     /gene="mutL"
                     /locus_tag="VVE35_000191"
     CDS             1448..3397
                     /gene="mutL"
                     /locus_tag="VVE35_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU3060451.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAQETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDNHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3723..4190)
                     /locus_tag="VVE35_000192"
     CDS             complement(3723..4190)
                     /locus_tag="VVE35_000192"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU3060452.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4191..5396)
                     /locus_tag="VVE35_000193"
     CDS             complement(4191..5396)
                     /locus_tag="VVE35_000193"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU3060453.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLASQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5416..6051)
                     /gene="ribE"
                     /locus_tag="VVE35_000194"
     CDS             complement(5416..6051)
                     /gene="ribE"
                     /locus_tag="VVE35_000194"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU3060454.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6036..7136)
                     /gene="ribD"
                     /locus_tag="VVE35_000195"
     CDS             complement(6036..7136)
                     /gene="ribD"
                     /locus_tag="VVE35_000195"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU3060455.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LIQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7271..7385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7542..8135
                     /gene="ruvA"
                     /locus_tag="VVE35_000196"
     CDS             7542..8135
                     /gene="ruvA"
                     /locus_tag="VVE35_000196"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU3060456.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8145..8708
                     /locus_tag="VVE35_000197"
     CDS             8145..8708
                     /locus_tag="VVE35_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU3060457.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHLVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8705..9382
                     /locus_tag="VVE35_000198"
     CDS             8705..9382
                     /locus_tag="VVE35_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3060458.1"
                     /translation="MMSNKNKGILIFVILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9537..10568)
                     /locus_tag="VVE35_000199"
     CDS             complement(9537..10568)
                     /locus_tag="VVE35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU3060459.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIETFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10823..11506)
                     /locus_tag="VVE35_000200"
     CDS             complement(10823..11506)
                     /locus_tag="VVE35_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU3060460.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11518..12462)
                     /locus_tag="VVE35_000201"
     CDS             complement(11518..12462)
                     /locus_tag="VVE35_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU3060461.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12594..15425
                     /gene="uvrA"
                     /locus_tag="VVE35_000202"
     CDS             12594..15425
                     /gene="uvrA"
                     /locus_tag="VVE35_000202"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU3060462.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKIQKDGYVRVRVDGEVYDVTEVLELSKSRQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15418..16479
                     /locus_tag="VVE35_000203"
     CDS             15418..16479
                     /locus_tag="VVE35_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU3060463.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16611..17009
                     /gene="spx"
                     /locus_tag="VVE35_000204"
     CDS             16611..17009
                     /gene="spx"
                     /locus_tag="VVE35_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU3060464.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17075..17644
                     /locus_tag="VVE35_000205"
     CDS             17075..17644
                     /locus_tag="VVE35_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3060465.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17731..17997
                     /locus_tag="VVE35_000206"
     CDS             17731..17997
                     /locus_tag="VVE35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU3060466.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18001..18420
                     /gene="ruvX"
                     /locus_tag="VVE35_000207"
     CDS             18001..18420
                     /gene="ruvX"
                     /locus_tag="VVE35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU3060467.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18436..18741
                     /locus_tag="VVE35_000208"
     CDS             18436..18741
                     /locus_tag="VVE35_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU3060468.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18948..20198
                     /locus_tag="VVE35_000209"
     CDS             18948..20198
                     /locus_tag="VVE35_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU3060469.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20195..20740
                     /locus_tag="VVE35_000210"
     CDS             20195..20740
                     /locus_tag="VVE35_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU3060470.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKSIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            20886..22418
                     /gene="cls"
                     /locus_tag="VVE35_000211"
     CDS             20886..22418
                     /gene="cls"
                     /locus_tag="VVE35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU3060471.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22496..22639
                     /locus_tag="VVE35_000212"
     CDS             22496..22639
                     /locus_tag="VVE35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060472.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22676..24214
                     /locus_tag="VVE35_000213"
     CDS             22676..24214
                     /locus_tag="VVE35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU3060473.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     FVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24334..26541
                     /gene="nrdD"
                     /locus_tag="VVE35_000214"
     CDS             24334..26541
                     /gene="nrdD"
                     /locus_tag="VVE35_000214"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU3060474.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26553..26693
                     /locus_tag="VVE35_000215"
     CDS             26553..26693
                     /locus_tag="VVE35_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060475.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26737..27243
                     /locus_tag="VVE35_000216"
     CDS             26737..27243
                     /locus_tag="VVE35_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060476.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27236..27826
                     /gene="nrdG"
                     /locus_tag="VVE35_000217"
     CDS             27236..27826
                     /gene="nrdG"
                     /locus_tag="VVE35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU3060477.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27823..28440
                     /locus_tag="VVE35_000218"
     CDS             27823..28440
                     /locus_tag="VVE35_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU3060478.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28706..29014
                     /gene="rpsJ"
                     /locus_tag="VVE35_000219"
     CDS             28706..29014
                     /gene="rpsJ"
                     /locus_tag="VVE35_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU3060479.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29231..29857
                     /gene="rplC"
                     /locus_tag="VVE35_000220"
     CDS             29231..29857
                     /gene="rplC"
                     /locus_tag="VVE35_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU3060480.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29882..30505
                     /gene="rplD"
                     /locus_tag="VVE35_000221"
     CDS             29882..30505
                     /gene="rplD"
                     /locus_tag="VVE35_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU3060481.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30505..30801
                     /locus_tag="VVE35_000222"
     CDS             30505..30801
                     /locus_tag="VVE35_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU3060482.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30819..31652
                     /gene="rplB"
                     /locus_tag="VVE35_000223"
     CDS             30819..31652
                     /gene="rplB"
                     /locus_tag="VVE35_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU3060483.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31756..32037
                     /gene="rpsS"
                     /locus_tag="VVE35_000224"
     CDS             31756..32037
                     /gene="rpsS"
                     /locus_tag="VVE35_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU3060484.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32049..32393
                     /gene="rplV"
                     /locus_tag="VVE35_000225"
     CDS             32049..32393
                     /gene="rplV"
                     /locus_tag="VVE35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU3060485.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32406..33059
                     /gene="rpsC"
                     /locus_tag="VVE35_000226"
     CDS             32406..33059
                     /gene="rpsC"
                     /locus_tag="VVE35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU3060486.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33063..33476
                     /gene="rplP"
                     /locus_tag="VVE35_000227"
     CDS             33063..33476
                     /gene="rplP"
                     /locus_tag="VVE35_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU3060487.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33486..33692
                     /locus_tag="VVE35_000228"
     CDS             33486..33692
                     /locus_tag="VVE35_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU3060488.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33717..33977
                     /gene="rpsQ"
                     /locus_tag="VVE35_000229"
     CDS             33717..33977
                     /gene="rpsQ"
                     /locus_tag="VVE35_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU3060489.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34003..34371
                     /gene="rplN"
                     /locus_tag="VVE35_000230"
     CDS             34003..34371
                     /gene="rplN"
                     /locus_tag="VVE35_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU3060490.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34449..34754
                     /gene="rplX"
                     /locus_tag="VVE35_000231"
     CDS             34449..34754
                     /gene="rplX"
                     /locus_tag="VVE35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU3060491.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34778..35320
                     /gene="rplE"
                     /locus_tag="VVE35_000232"
     CDS             34778..35320
                     /gene="rplE"
                     /locus_tag="VVE35_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU3060492.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35338..35607
                     /gene="rpsN"
                     /locus_tag="VVE35_000233"
     CDS             35338..35607
                     /gene="rpsN"
                     /locus_tag="VVE35_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU3060493.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35892..36290
                     /gene="rpsH"
                     /locus_tag="VVE35_000234"
     CDS             35892..36290
                     /gene="rpsH"
                     /locus_tag="VVE35_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU3060494.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36482..37018
                     /gene="rplF"
                     /locus_tag="VVE35_000235"
     CDS             36482..37018
                     /gene="rplF"
                     /locus_tag="VVE35_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU3060495.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37102..37458
                     /gene="rplR"
                     /locus_tag="VVE35_000236"
     CDS             37102..37458
                     /gene="rplR"
                     /locus_tag="VVE35_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU3060496.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37476..37970
                     /gene="rpsE"
                     /locus_tag="VVE35_000237"
     CDS             37476..37970
                     /gene="rpsE"
                     /locus_tag="VVE35_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU3060497.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37942..38166
                     /gene="rpmD"
                     /locus_tag="VVE35_000238"
     CDS             37942..38166
                     /gene="rpmD"
                     /locus_tag="VVE35_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU3060498.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38311..38751
                     /gene="rplO"
                     /locus_tag="VVE35_000239"
     CDS             38311..38751
                     /gene="rplO"
                     /locus_tag="VVE35_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU3060499.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38764..40074
                     /gene="secY"
                     /locus_tag="VVE35_000240"
     CDS             38764..40074
                     /gene="secY"
                     /locus_tag="VVE35_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU3060500.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40225..40863
                     /locus_tag="VVE35_000241"
     CDS             40225..40863
                     /locus_tag="VVE35_000241"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU3060501.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40980..41198
                     /gene="infA"
                     /locus_tag="VVE35_000242"
     CDS             40980..41198
                     /gene="infA"
                     /locus_tag="VVE35_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU3060502.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41223..41339
                     /gene="rpmJ"
                     /locus_tag="VVE35_000243"
     CDS             41223..41339
                     /gene="rpmJ"
                     /locus_tag="VVE35_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU3060503.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41357..41722
                     /gene="rpsM"
                     /locus_tag="VVE35_000244"
     CDS             41357..41722
                     /gene="rpsM"
                     /locus_tag="VVE35_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU3060504.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41740..42123
                     /gene="rpsK"
                     /locus_tag="VVE35_000245"
     CDS             41740..42123
                     /gene="rpsK"
                     /locus_tag="VVE35_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU3060505.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42166..43101
                     /locus_tag="VVE35_000246"
     CDS             42166..43101
                     /locus_tag="VVE35_000246"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU3060506.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43113..43499
                     /gene="rplQ"
                     /locus_tag="VVE35_000247"
     CDS             43113..43499
                     /gene="rplQ"
                     /locus_tag="VVE35_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU3060507.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43764..44030
                     /locus_tag="VVE35_000248"
     CDS             43764..44030
                     /locus_tag="VVE35_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU3060508.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44040..45377
                     /locus_tag="VVE35_000249"
     CDS             44040..45377
                     /locus_tag="VVE35_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU3060509.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQIGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTSAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45621..46313
                     /locus_tag="VVE35_000250"
     CDS             45621..46313
                     /locus_tag="VVE35_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3060510.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46416..48061)
                     /locus_tag="VVE35_000251"
                     /pseudo
     CDS             complement(46416..48061)
                     /locus_tag="VVE35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(48051..49142)
                     /locus_tag="VVE35_000252"
     CDS             complement(48051..49142)
                     /locus_tag="VVE35_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060511.1"
                     /translation="MSEIEIINAKKIYHDVSVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESRDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(49155..50276)
                     /locus_tag="VVE35_000253"
                     /pseudo
     CDS             complement(49155..50276)
                     /locus_tag="VVE35_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(50493..50690)
                     /locus_tag="VVE35_000254"
     CDS             complement(50493..50690)
                     /locus_tag="VVE35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3060512.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKHLHISPKELKLLGRIVK"
     gene            complement(50765..>51100)
                     /locus_tag="VVE35_000255"
     CDS             complement(50765..>51100)
                     /locus_tag="VVE35_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU3060513.1"
                     /translation="NSIKHKKVTGVFNQMIIKNIHYAFSQNKTIVLRIPVIPNFNNSL
                     EDAEKFATLFNSLNIDQVQLLPFHQFGENKYRLLNRKYEMDGVNALHPEDLIDYQKVF
                     LNHHINCYF"
BASE COUNT        15400 a         9599 c        11155 g        14946 t
ORIGIN      
        1 aagatctgaa ataactgtta tgatatagca gattgaaact agaatagtac acctttgctt
       61 ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttatcctgt tcttatttca
      121 tttcactata gtaaggtgca gcaggataaa gaaaaaccga gaagtttctt tctcggattt
      181 gcttattatg aggttcctat tttctatgct ccaagagttg attgatgtga tctacttttt
      241 tagcctctct tttatagaga atagcccaga tgatgagata gacaatcgca aactcgataa
      301 tgagctggag gtagaaaatc cagtggaaag ggaaccaacc agcaagagtt gctagtggga
      361 caaagccagc cagcataagg aagaaatgag tcagagtggc acgaagcatg ctccagtcac
      421 ggctgaataa gcggttgcca aagttgaaga gcataccgat ggctgaccag ataagtgtgc
      481 agtagagcaa gaccagggca ccgtgaacct gatgctgatc catcacttgg ccgatgagag
      541 agtagggatt tagtggtgcg taggtatttg gtgcataaat gagtgaaaag aggatggaga
      601 ggatgaggcc gataagaaca ccggcagctg catcgtgaaa tacttgtttt ttcatagttc
      661 taatttctcc ttgatggttt ttagataacg gcgtgaagag taggtgaagc tttcgttttt
      721 caagaaaatt tctaccagac cgtttggcgt gagcttgagg tgagagatgg aatcgatatt
      781 gatgatttct gattgggaaa tttggataaa attgcttggc aagagtttaa gaacctgata
      841 gagtcgcaaa tcaatgctgt aggtctgact cgcggtttct gctagaacct tccgattctc
      901 gatatagaag cgctgaatct tgccaatctc aactagatag acctgatcat cgattttccc
      961 tttgattttt tctctttggt ccagattttc tgctaactcg atgactttct ggactttatc
     1021 tgtcggctga ggtgcttgga caatcagctt ttcctcctcg taagtctcac taatctgtag
     1081 ttctactttc ataaatttct ctccttgttt ttcatacaaa attaatctca cttcttgtac
     1141 acctttatta atactcttca aaaatctctt caaaccacgt cagcgtcgtc ttgccgtagg
     1201 atagttgttg actttgtcag ttttatctac aacctcaaaa cagtgttttg aactgtctgc
     1261 gcctagcttt ctagtttgct ctttgatttt tattgagtat accactattt tactccctct
     1321 ggcaagggac tttgtctatg tggagggatt gggctcctat gtggtggagc ttttctgttc
     1381 tttctgaaat atggtataat agcactaatt aatttctagg aaaatagata cagaaagggg
     1441 ctgaaagatg tctcatatta ttgaattgcc agagatgctg gcaaaccaaa tcgcagctgg
     1501 agaggtcatt gaacgtcctg ccagtgtggt caaagagttg gtagaaaatg ccattgacgc
     1561 gggctctagt cagattatca ttgagattga ggaagctggt ctcaagaagg ttcaaatcac
     1621 ggataacggt catggaattg cccacgatga ggtggagttg gccctgcgtc gccatgcgac
     1681 cagtaagata aaaaatcaag cagatctctt tcggattcgg acgcttggtt ttcgtggtga
     1741 agccttgcct tctattgcgt ctgttagtgt cttgactctg ttaacggcgg tggatggtgc
     1801 tagtcatgga accaagttag tcgcgcgtgg gggtgaagtt gaggaagtca tcccagcgac
     1861 tagtcctgtg ggaactaagg tttgtgtgga ggatctcttt ttcaacacgc ctgcccgtct
     1921 caagtatatg aagagccagc aagcggagtt gtctcatatc attgatattg tcaaccgtct
     1981 gggcttggcc catcctgaga tttcttttag cttgattagt gatggcaagg aaatgacgcg
     2041 gacagcaggg actggtcaat tgcgccaagc aatcgcaggg atttacggtt tggtcagtgc
     2101 caagaagatg attgaaattg agaactctga cctagatttc gaaatttcag gttttgtgtc
     2161 cttgcctgag ttgactcggg ctaaccgcaa ttatatcagc ctcttcatca atggtcgtta
     2221 tatcaagaac ttcctgctca atcgtgctat tttggatggt ttcggaagca agcttatggt
     2281 tggacgtttt ccactggctg tcattcacat ccatatcgac ccttatctag cggatgtcaa
     2341 tgtgcatcca actaagcaag aggtgcggat ttccaaggaa aaagaactga tgactctggt
     2401 ttcagaagct attgcaaata gtctcaagga acaaaccttg attccagatg ccttggaaaa
     2461 tcttgccaaa tcgaccgtgc gcaatcgtga gaaggtggag caaactattc tcccactcaa
     2521 agaaaatacg ctctactatg agaaaactga gccgtcaaga cctagtcaaa ctgaagtagc
     2581 tgattatcag gtagaattga ctgatgaagg gcaggatttg accctgtttg cccaggaaac
     2641 cttggaccga ttgaccaagc cagcaaaact gcattttgca gagagaaagc ctgctaacta
     2701 cgaccagcta gaccatccag agttagatct tgctagcatc gataaggctt atgacaaact
     2761 ggagcgagaa gaagcatcca gcttcccaga gttggagttt ttcggacaaa tgcacgggac
     2821 ttatctcttt gcccaagggc gagatggact ttacatcata gatcagcacg ctgctcagga
     2881 acgggtcaag tacgaggagt accgtgaaag cattggcaat gttgaccaga gccagcagca
     2941 actcctagtg ccctatatct ttgaatttcc tgcggatgat gccctgcgtc tcaaggaaag
     3001 aatgcctctc ttagaggaag tgggcgtctt tctagcagag tacggagaaa atcaatttat
     3061 tctacgtgaa catcctattt ggatggcaga agaagagatt gaatcaggca tctatgagat
     3121 gtgcgacatg ctccttttga ccaaggaagt ttctatcaag aaataccgag cagagctggc
     3181 tatcatgatg tcttgcaagc gatctatcaa ggccaatcat cgtattgata atcattcagc
     3241 tagacaactc ctctatcagc tttctcaatg tgacaatccc tataactgtc ctcacggacg
     3301 tcctgttttg gtgcatttta ccaagtcgga tatggaaaag atgttccgac gtattcagga
     3361 aaatcacacc agtctccgtg agttggggaa atattaaaag tataaaaaag tctgggaaaa
     3421 attttcaaaa tcagaaaaac gcataaaatc aggtgttcaa aaaccttgat ttttatgcgt
     3481 tttatcatgg aaatagttac ttcatttttt cctaattctt ttcgaaactc tttttaaacg
     3541 acgtcagttt tatcagtaat ctcaaaacag tgttttgagc taattttgcc agttttgtct
     3601 gtaacatcga agttgtgttt taccactctg cgactggttt cctagtttgc tctatgattt
     3661 tcacagagta ttaaattgcg attttgccaa gtttctttat tcgtctaaaa gtagagtctg
     3721 ttctatgcgt ctaatgtacg aattaggttg accatttcaa tagctccttg tgcacactca
     3781 gaacccttat ttcctgcttt ggtaccagct cgttctatgg cttgttcaat tgtatctgtc
     3841 gttagcacac caaacataac aggaatttcg ctatttaaac tgatttgggc gattccctta
     3901 gatacctcgc tacatacata atcataatga cttgtattcc ctctaatgac agctcccaag
     3961 cagataattg catcatattt tttacttttt gccatttttg atgcaatcag tggtatttca
     4021 aaagctcctg gaacccaggc tacctctata tctttctcgt ttacattctc tcttttgaga
     4081 ttatctagtg ctccagataa taattttgaa gttataaatt cattaaatct cgctacaaca
     4141 atacctattt taatattgtt tgctactaaa ttaccttcat aagtgttcat ttatttttcc
     4201 tccatattta aaatgtgacc cattcgattt ttctttgttt ctaaataaaa actatcgtaa
     4261 ggattggctt ctatttcgat tgatattcta ctggaaatgg taattccata tttttctaac
     4321 tgttcaacct tgtcaggatt atttgtcagt aaatgaagtg actgaagtcc cagatcttta
     4381 agcatttgtg ctccaatatg atattctctt aaatcacctt caaagcctaa tgcaagattg
     4441 gcatcaagcg tatccatgcc ttgatcttgt aaatgatagg cttttaattt attgataagt
     4501 ccaattcctc gtccctcctg tcgcaagtaa agtaagacac ccgaaccatt ctcaacaatc
     4561 attttcatag ctttatcgaa ttgctgtcca caatcgcaac gtaaagagcc taaaacatct
     4621 cctgttaaac attcggagtg gacccgacat aatacattgg cttcatcctc tatatttccc
     4681 ataataagag caagatgatg ttccccattt agtttatcta tatagctaat tgctttgaaa
     4741 ttaccgtatc tagtaggcat attgacagtt gaaactcgtt ctatcagctg atcatatact
     4801 tttctatatt cttgtaattc tttgatggta attagtggaa tgttgtgttt cttcgagaac
     4861 tgaattaaat catctgttct catcattttg ccatcatgat tcattatttc acaacatagg
     4921 ccacactctt ttagtccagc taattttaat aaatcaacag ttgcttctgt gtgtccattt
     4981 ctttctagga caccaccttt ttttgcaatt aaaggaaaca tgtgtcctgg cctgcgaaaa
     5041 tcagagggtg ttatatcttc agctacacac atacgtgcgg tcagtcctct ttcctcggca
     5101 gaaatacctg tggtcgtttc tttataatca attgaaactg taaaagcagt cttatgatta
     5161 tctgtattgt tttcaaccat aggtgaaagc attaattgac tagctaaact ttcgctcata
     5221 ggcatacaaa ttaatccttt ggcataagta gccataaaat taacattttc tgttgtagct
     5281 gcttgtgcag aacaaattaa gtctccttca ttttctctat ccttgtcgtc tataacaaga
     5341 acaagtcgtc ccttctgcaa tgcttctaat gcttcttgta tttttcgata ttccattgac
     5401 tgattatcct ttctgctaaa atccattttg atataatagt tccttagata tttctgattt
     5461 tggagagtta tccatcagtt tttgcacata tttacctaag atatcatttt caagatttac
     5521 tgtactcccg acttgtttac tcttaagaat ggtttgttcc aaggtatgag ggataacaga
     5581 tactgaaaag tttactttgg agactttagc gacagtcaga ctaatgccgt caattgtaat
     5641 agatcctttt tcaactatta aatctaaaat ttctttttgt gtgttgattt gataccatac
     5701 agcattatca tcttttttta ttgacgagat ttttcctgta ccatcaatgt gtcctgtaac
     5761 gacgtgaccc ccaagtcgac cgttgacaga taaggctctt tctagattca cctcacttcc
     5821 atgttttaat agagtaagag ctgttcgact ccatgtttca ttcattacat caactgtaaa
     5881 ggattgatga ttgaaatgag taactgtaag acagatacca tttactgcta tactatcgcc
     5941 taaatggata tccgttaata tttttgaggc tttaattgat agtttacaat tacgagagtc
     6001 tttctgtatt ctttcaactt ttccgatttc ttcaattatt cctgtgaaca tggataaatc
     6061 acttcacttt ctatgagata gtcatttcct atttgagaaa aagcataagg tttcaatcta
     6121 atagcgtcat ttggcaaaga aatgccttca cctccgacag gaaacttggc actgcctcca
     6181 aaaatttttg gtgcaatata tattttcagc tcatcaacaa tttgttgttc caaagcactc
     6241 caattcatta gactgccccc ttctagaact aggctatcaa tctgcatgtt tcctagatgt
     6301 tgtattaaac tcgataagtc tatatgattg ccttttttct ttatggaaag tatttcacag
     6361 ccatgatttt gatatagctt cattttattt ttgtcttcag aggaagtggc aatgtaagtt
     6421 ttaatatcat ttgctgtttt tacgatttta gaggtaagag gagttcgtaa atgtgtatcg
     6481 catatgatac ggataggatt tttcccttcc tccaatctac atgtcagcaa aggatcgtct
     6541 tgaataacag tattgactcc caccataatt gcactaacat ggtgtcgtaa ctgatgcaca
     6601 tgctttcttg cttcttcttc agtaatccat ttggattgat ttgttttagt ggctattttt
     6661 ccatccattg acattgcata tttcataaaa acatagggta catgctgggt aatatacttt
     6721 ctaaaacttt ttattaagtt aagacactca ttttctaaaa ttccaacagt aacttgaaga
     6781 ttattttcct caagtatctt tactcctttt ccagatacaa taggattaca gtctaggctt
     6841 ccaatgacta ctcttgtaat accactatcg attatagcat ctatacaggg aggtgttttc
     6901 ccgaagtgac aacagggttc aagtgttaca taaagcgtcg ctccgacagg ggattctcta
     6961 cagtttttaa gagcatttct ctcagcatgt gggccaccaa aaaactcatg ataaccttgt
     7021 ccgatgatgt gattatcttt tacaataact gcgccgacca taggattggg attgacgtaa
     7081 ccagcccctt tttgtgccag ttttattgct aatttcatat attttgaatc gctcatctca
     7141 ctacctccaa aaaaaatata ccttgaatag gggactactc aaggcataca aaagaaaact
     7201 tatgcgatta acaaaaatac tctgaaatga caagtaatca tttcagagca cgcaaaaagc
     7261 acaaatatac ttttatcttc tttcatccag actatactgt cggctttgga atttcaccaa
     7321 atcatgcctt tcggctcgtg ggctatacca ccggtaggga attgcaccct gccctgaaga
     7381 tagttattca attacagatg attatagtac ttaattttga atatgtcaac agataaatac
     7441 cgattgtttt tgatatactg tatttgtgat aatcgattct cgctcctcgg ataaagaaaa
     7501 tatgatatac tagataaacg aaataagaga gaaggaatac tatgtacgca tatttaaaag
     7561 gaatcattac caaaattact gccaaataca ttgttcttga aaccaatggt attggttata
     7621 tcctgcatgt ggccaatcct tatgcctatt caggtcaggt taatcaggag gctcagattt
     7681 atgtgcatca ggttgtgcgt gaggacgccc atttgcttta tggatttcgc tcagaggatg
     7741 agaaaaagct ctttcttagt ctaatttcgg tctctgggat tggtcctgta tcagctcttg
     7801 ctattatcgc tgctgatgac aatgctggct tggttcaagc cattgaaacc aagaacatca
     7861 cctacttgac caagttccct aaaattggca agaaaacagc ccagcagatg gtgctggact
     7921 tggaaggcaa ggtagtagtt gcaggagatg accttcctgc caaggtcgca gtgcaagcaa
     7981 gtgctgaaaa ccaagaattg gaagaagcta tggaagccat gttggctctg ggctacaagg
     8041 caacagagct caagaaaatc aagaaattct ttgaaggaac gacagataca gctgagaact
     8101 atatcaagtc ggcccttaaa atgttggtca aataggagca gagaatgaca aaacgttgtt
     8161 cgtgggtcaa gatgaccaac ccgctctaca tcgcctatca tgatgaggag tggggtcagc
     8221 ccctccatga tgaccaagcg ttgtttgagt tgttgtgtat ggaaacctat caggcaggcc
     8281 tgtcttggga aacggtactc aacaaacgcc aagctttccg agaagccttt catagctatc
     8341 aaattcactt agtcgcagag atgactgaca ctgaattgga agccatgctg gagaatccag
     8401 ctatcattcg aaatagagcc aagatttttg ctacacgcgc taacgcccaa gcttttctac
     8461 agttacaggc agagtacggc tcttttgatg cctatctttg gtcttttgtt gaggggaaaa
     8521 ctgtcgttaa cgatgttcct gattatcgcc aagccccagc taaaacaccc ttatctgaga
     8581 aattagccaa agatctcaaa aaacgaggct tcaagttcac aggcccagtc gccgtattgt
     8641 cttttctaca ggctgcaggg ctagttgatg accacgagaa tgattgtgag tggaaaggtc
     8701 ttaaatgatg tctaacaaaa ataagggaat tctgattttt gtgattctct atacagtcct
     8761 ctttgtgttt gatggcgtta aattgctggc ttctttaatg ccatctgcca ttgcaaatta
     8821 tcttgtttat gtagttttag ctttatatgg ctccttcttg ttcaaggata gattgatcca
     8881 acaatggaag gagattagaa agactaaaag aaaattcttc tttggagtct taacaggatg
     8941 gctctttctc attctgatga ctgttgtctt tgaatttgta tcagagatgt tgaagcagtt
     9001 tgtgggacta gatggacaag gtctaaatca gtctaatatt caaagtacct ttcaagaaca
     9061 accactactg atagctgttt ttgcttgtgt cattggacct ctggtagaag aattattttt
     9121 ccgtcaggtc ttattgcatt acttgcagga acggttgtca ggtttactaa gcattattct
     9181 ggtaggactt gtttttgctc tgactcatat gcacagtttg gctctatcag agtggattgg
     9241 tgcagttggt tacttaggtg gaggccttgc cttttctatt atttatgtga aagaaaaaga
     9301 gaatatctac tatcccctac ttgttcacat gttaagcaac agcctctcct taatcatttt
     9361 agctatcagt atagtaaaat gaaataagaa caggacaaat cgatcaggac agtcaaatcg
     9421 atttctaaca atgttttaga agtagaggtg tactattcta gtttcaattt gctgtaatat
     9481 gtgatgaaaa tgccagtaat aataccgaga aaaaagctga gaaacttttc ccagctttat
     9541 ttgttatagt caaagcgaat gacttgttcc tgtgcatcta catgagcatg aaccccaaag
     9601 ggtacaattg ctcttggagt tgcgtggccg acattcagat tatagacaat cgggatattg
     9661 ctgtcaatga tatccaatag tgcctcttta tagtcgtcat ggaaagtttc atccataggt
     9721 tttccgacca agagtccatt gatgaccgcg aatatgccag tgtcctttaa agttagcaac
     9781 atctttttga agtcttctgg cttaggcttt tcttcgcttg tttcgagcaa gaggattttc
     9841 ccttcccagt ctgacaagtc agggaaaagt ttgtattttt ggcagagttc cgtgctatct
     9901 gcgtatcgag agttgtcaaa gatatcgtag agggattcga ggcaaccacc gaggattttc
     9961 ccctcgaact gagcacttcc ttgcaacaag tcaaaacctg tatttgtatg actgacacga
    10021 ggtgttccca gggccgtggg actaaaatca gttcgttcct cataccaaac gtcactaggg
    10081 cggatttctg aaattcttcc cgtctcaatc aattctttaa agtagtgaag gctataggct
    10141 agcatttctt tgtctaattc acaaatgtct gctaaaaagg attgaccata aaaagtctcg
    10201 attcctaatt tatgcaacat gaggtggttc atggttgtat ccgagaagcc aagaaaaatt
    10261 ttttgtttga taaccttttg gagttggtca ttttcaaaaa gataaggtag caagcgatag
    10321 gtatcgtctc caccgatggc acataggatc atgtcgatgc tatcatcaga aaaggcatga
    10381 atcaaatcct ctgcacgagc ttcaggatgg tccttgataa agtctaatcc ttttaacgaa
    10441 tggggcaaaa agatgggatt gagtcccaga tccttgagac gttggatacc caagtccact
    10501 tcgtgtttga caaaatcctc tccgataatg ccactagata aactaacaat accaatagta
    10561 gaaaccatat ctcatcctcc tagaaataga ttgagcctat tttatcacaa aatataagag
    10621 aaagctatgt gggatttcag aagaatactt ttaatactct tcgaaaatct cttcaaacca
    10681 cgtcagtttt atctgcaacc tcaaagctgt gctttgagca acctgcagct agcttcctag
    10741 tttgctcttt gattttcatt gagtataact caaaaaagta agtgaaaaag caaccgcacc
    10801 tgcagttgct tttatcgttt tcttattctt gatagcgtgt tggtcctgca cttgcactac
    10861 ggatattcaa gcgtttcttg agatagaagc gaagggctag gagggctgct ccaataatag
    10921 ctagtggcaa tggatccagt actgggttaa ggctagctgg taggaagctt gttgcaaaga
    10981 agacaagcaa ccaaaggaac atagaagcta ggataactag tacagatttc cagaaaggtg
    11041 gacgttgact gcgatccata tctggtccat agtattggta aacaaagtag tacatcaagt
    11101 agaaggcaaa tccaccaacc agtccaacta atagaagagt aatcaatcca tagccgaaag
    11161 cttgatctgc cgcaaagaag gttgtgaggg cgctgacaag ggcaaagagg ctagtgatga
    11221 aaagagctga gtccataatc atcagttttg ggtcatcatt ttcttttgga tgctcttttt
    11281 cgtactgctc tttgacagtg aagctatgag cccaatgagt tggtgcgccg tatagggaac
    11341 gggcagttgt acctttagat tgctcctcaa ggatttgggg aatgacttcc tcaaaaatag
    11401 tctggatttc agcgtctgtt ttcccatctt tgatgaattg ttgggtagca atgtggacaa
    11461 actcttggtt tttcttagtt aatttttgta gatcaatttg agacatagga actcctctta
    11521 gaaccatttt ttatggatga gatagagagt gagcgagaca ctcatagcaa aggcgataaa
    11581 gacgattaac cagaaggcat ttggctctcc gtttagggga atttcattat ccttaaagtt
    11641 catcccgtag gcagaaaaga ccatggttgg gatggacatg acgatggtca caagggccaa
    11701 ggttttcatg atgttgttct ggttgttaga aatgatagag gcaaaggtct ctgtcataga
    11761 atgcaagacg tttccataaa tatctgccat ctcgatggcc tgttgggttt caatcagggt
    11821 gtcttcaagc aggtcttcgt cctcaaggta tttcttgata ttgctggttg aactggtcaa
    11881 tttcttaatc acgcgctcat ttgttttgag ggaggccttg aaatagacga tagttttttc
    11941 caattccatg agctcaatca attcttcatt acgagttgat tgatgcagtt gactttcgat
    12001 ttgttcactc ttgcggtcga ttgaacgaag ggctgttagg taaagctctg cattgcgata
    12061 aagaatttga aagataaaac gtgaacgcat gaaggtatag aaattacgca atcgacggtt
    12121 gataaagaca tcaaggacag gtagtggttc caaacacgta gtgataatgg tttcctcagt
    12181 gatgataata ccaagcggga tggttacgta gtaggtgcgg ttatttcttt cctccgtgac
    12241 cggcacgtct acgataatca gggtatactc gtcttcaatg gtaatacgag acatttcttc
    12301 cgcatcgagc ggtgctcgaa ggtcggcaat atcaatatcg aaggtgttgg cgatttcgag
    12361 tgattcattt tgagtcggat tgacgagatt gatccaagta cccggttcaa gcgtatcgat
    12421 ctctttaaat tcagttgttg tagagagaaa aacttgtttc atatccctta tcctttctca
    12481 tttttcagat tttttcacac tgtactatta tactacaaaa tcggcctttt gggtaataga
    12541 atctcacttt ttttggtata atggtaagca ataatggact agaaagaaca aagatgcaag
    12601 ataaaattgt cattcatggg gcgcgtgccc ataatttaaa aaatattgat gtggagattc
    12661 cgcgagacaa gttggttgtc gtgactggct tgtcaggttc agggaagtcc agtctggctt
    12721 ttgataccct ctatgcggag ggacaacgtc gctatgtaga gagtttgtcc gcctacgctc
    12781 gtcagttctt gggaaatatg gaaaaaccag atgtagatgc cattgatggt ctcagcccag
    12841 ctatttctat cgaccagaaa acgactagca aaaaccctcg ctcgacggtg ggaacaacga
    12901 ctgaaatcaa tgactatctg cgtctcctct acgcgcgtgt ggggacgcct tactgtatca
    12961 acgggcatgg ggctatcaag gcttcttctg tggagcaaat cgttgataag gttttggagt
    13021 tacctgaacg ccagcgcctg cagattttgg cccctgtcat ccgtaagaaa aaaggacagc
    13081 ataagagcgt tatcgagaag attcagaaag atgggtatgt tcgtgtccgt gtggatgggg
    13141 aagtctatga tgtgaccgaa gtgctagagt tgtccaagag cagacaacac aatattgatg
    13201 tcgtagttga ccgtattgtc atcaaggagg gtattcgtag ccgtctcttt gattccattg
    13261 aagctgccct tcgtattgca gaaggttatg tcattatcga caccatggac gattcggagt
    13321 tgctcttctc tgagcattat gcctgtccag tttgtggttt tactgtccca gagttagagc
    13381 ctcgtctctt ctctttcaat gctccttttg gctcttgtag tgagtgtgac ggcttgggca
    13441 tcaagctgga ggtggatact gatttggtag tgccagatgc cagcaaaacc ttacgtgagg
    13501 gagcgctagc accttggaat cctatctcat ccaactacta tccaaacatg ttagagcagg
    13561 ccatgaaagt ctttggagtg gctatggata agccttttga ggacctgtca gaagaagata
    13621 agaacttgat tctctatggg tcagatggta aggaattcca tttccactat gagaatgaat
    13681 ttggtggcgt gcgcgatatc gacattccgt ttgagggagt tatcaataat atcaaacgtc
    13741 gttaccatga aaccaatagc gattacactc gcactcagat gcgtctctac atgaatgagc
    13801 tgacctgcgg aacctgtcaa ggctatcgtc tcaatgacca ggccttgtct gtccgtgtgg
    13861 gcggccagca agggccacat attggagaaa tctcagacct gtctatcgct gaccacttgg
    13921 acttggtgag ccagttaact ttgtctgaaa atgaagccat cattgctcgt cccattctca
    13981 aggaaatcaa ggatcgtttg accttcctta ataatgtggg tcttaactat ctaaccctgt
    14041 cacgttcagc aggaaccctt tcaggtgggg aaagtcagcg cattcgtttg gcaacccaga
    14101 ttggttccaa cctatcaggc gtcctctata tcctagacga gccgtcaatc ggtcttcacc
    14161 agagggacaa tgaccgcctg attgccagtc tgaaaaagat gcgtgacttg ggcaatactc
    14221 ttatcgtggt ggaacacgac gaagatacca tgcgtgaagc tgattatctg attgacgttg
    14281 gtcctggtgc cggtgttttt ggtggtgaga ttgttgcagc aggtacacct aagcaagtgg
    14341 ctcgcaacag caagtctatc acaggtcagt acttgtcagg caaacgtgtc atcccagtac
    14401 cagaagagcg ccgtgtcgga aatggtcgtt ttattgaagt gataggagcg cgtgagaaca
    14461 acttgcaaaa tgtcacggct cgctttccac taggaaaatt tatcgcagtg acaggtgtgt
    14521 cgggttcagg gaaatcgact ctaatcaaca gcattctcaa aaaagccatt gcccagaagc
    14581 tcaaccgtaa ttcagacaaa cctggtaagt ttaagactat tacagggatt gagcatgtcg
    14641 accgtttgat tgatattgac cagagcccta tcggacggac gccgaggtct aacccagcta
    14701 cctatacagg agtttttgac gatatacgtg acctttttgc tcagacaaat gaagccaaga
    14761 ttcgaggcta caagaagggg cgcttcagtt tcaacgtcaa gggaggtcgc tgtgaagctt
    14821 gctcaggtga cgggattatc aagattgaga tgcacttctt gccagatgtt tatgtggctt
    14881 gtgaagtttg ccacggaacc cgctataaca gtgaaaccct agaagttcac tacaaggaaa
    14941 agaatatttc gcaggtcttg gatatgacgg tcaacgatgc ggtagaattt ttccaacaca
    15001 ttccgaaaat tcaacgcaaa ctccagacca tcaaggatgt aggtctaggc tatgtgacct
    15061 tgggacagcc agctaccacc ctttctgggg gagaagccca acgtatgaag ttagctagcg
    15121 aactccacaa acgctcgaca ggaaaatctt tctacattct ggatgagccg acgacagggc
    15181 ttcatacaga ggacattgct cgcctgctca aggttttagc tcgctttgta gacgatggca
    15241 atacagtcct cgtcatcgag cacaatctgg atgttatcaa gacggcagac catatcattg
    15301 acttgggacc tgagggcggt gtcggtggtg gaaccatcat cgtaacagga actccagaag
    15361 aagtagctgc caacgaagcc agctatacag gacactattt gaaaggaaag ttacatcatg
    15421 aataaacgcg tacaagcatt tctagctaaa atgcaagaaa aagaactaga tggtatcatc
    15481 atcaacaatc ttaaaaacgt ctattatttg actggttttt ggggctcaaa cggaacagtc
    15541 tttatcagcc gtgaccgtca ggtcttagtg acagactctc gctatatcat cgcagctaag
    15601 caagaaacca gtggttttga gattgtggct gatcgtgatg aattggctgt cattgcagga
    15661 attgttaagg acatgggctt gactcgtatc ggttttgaag atgagatttc agtgtcttat
    15721 taccaccgta tgcaggcagc ttttgcaggt ttggacttgc ttccacaaac tcagtttgtg
    15781 gaaggtcttc gtatgattaa ggatgaggca gagattgcag cgattcgcaa ggcttgttct
    15841 atctcagacc aagctttccg cgatgcgctt gactttatca aaccaggaaa aactgaaatt
    15901 gagattgcca acttccttga tttccgcatg cgtgagttgg gagcatctgg cttatctttt
    15961 gatacgatcc tagctagcgg tatcaattct tctaaacccc atgcccatcc aatgcacaaa
    16021 ccagtggagt tgggagaagc cattaccatg gacttcggct gtctctatga ccactatgtc
    16081 agtgatatga cccggactat ctatctaggg catgttagcg atgagcaggc agagatttac
    16141 aatacggttc taaaagctaa ccaagccttg attgaccaag ctaaggcagg cttaggtttc
    16201 cgtgactttg acaaaatccc tcgtgatatt atcattgagg caggttatgg tgactacttt
    16261 actcacggca ttggccacgg tattggtctg gatatccatg aggaacccta ctttagtcag
    16321 acttctacag aaactattaa gacaggtatg gccttgaccg atgaaccagg tatctatatc
    16381 gaaggcaaat atggcgttcg tatcgaggat gatatcctga ttacagagac aggttgtgaa
    16441 ttattgaccc tagctccaaa agagttgata gtcatttagt tgtttgtcaa gaaaaaagta
    16501 cgaaaatgac gaaaaaagtc aaaaaaaatt aaaaataggt cgcaagtcgg atgtttttta
    16561 tggtataata gactaaactg atagtaacat gtagcgaaag gggtaggtac atgattaaaa
    16621 tttatacagt ctcaagttgt actagctgta aaaaagcaaa aacctggctc aatgcccacc
    16681 agttaagtta taaagaacaa aaccttggta aagaaggaat tacgagagaa gaattactgg
    16741 atattctaac caaaacagat aacggaatag ccagcatcgt ttcgtctaaa aatcgctatg
    16801 ccaaagccct tggagtggat attgaagatt tgagtgtcaa tgaagttctc aatctgatta
    16861 tggaaacacc gagaatttta aagagcccaa tccttgtaga tgaaaaacgc ctgcaagttg
    16921 gctacaagga agacgatatt cgtgccttcc taccacgctc tgtccgtaat gtagaaaatg
    16981 cagaagcacg tttgcgtgca gctctataaa catcaaggct gggagcactt cccagtctta
    17041 ttctatttta atttcaaaaa gaaagaagaa agaaatgaaa aaaatagttc ttgttagtct
    17101 agctttcctt tttgtcctgg ttggttgcgg acagaaaaaa gaaactggac cagctacaaa
    17161 aacagaaaaa gatacgcttc agtcggcatt gccagttatt gaaaatgctg agaagaatac
    17221 agttgtaact aagactttgg tcttgcccaa gtcagatgat ggtagccagc agacacaaac
    17281 aattacttac aaagacaaga cttttttgag tctagctatc caacaaaaac gtccagtctc
    17341 tgatgagttg aagacttata ttgaccaaca tggagtggag gaaactcaaa aagctcttct
    17401 tgaagcggag gagaaggata agtctatcat tgaagctcgt aaattggcag gtttcaaact
    17461 tgaaacaaaa ctattgagcg caacggaact tcaaacaacg actagttttg attttcaagt
    17521 tctggatgtc aagaaggctt cccagttgga acatctgaag aatattggtt tggaaaatct
    17581 tttgaaaaat gaaccaagca aatatatttc agatagattg gcaaatggcg cgacagaaca
    17641 atagaaaatc caaataaata gactgcctga attgtagaag gtaaggaatt atgctataat
    17701 ggtactattc taaggaaaga gggtgttaga atgggattta ctgaagaaac agtacgtttt
    17761 aaattggacg attccaataa aaaagaaatt agcgaaactt tgacagatgt ttatgcttcg
    17821 ttgaacgata agggttacaa cccaattaac caaatcgtag gttacgtatt gagtggagac
    17881 cctgcctacg ttcctcgtta taataatgca cgaaatcaaa tccgtaagta tgagcgtgat
    17941 gaaatcgttg aggaattggt tcgctactac ctcaaaggac aaggagtcga tctataacct
    18001 atgagaatta tgggattgga cgtcggttca aaaacggtag gggtggcgat tagcgatccg
    18061 cttggtttta cagctcaagg gcttgaaatc atccagataa atgaagaaca aggccaattt
    18121 ggttttgacc gcgttaagga attggttgat acttacaagg tggaacgatt tgtagtgggc
    18181 ttgcctaaaa acatgaacaa tacaagtgga ccgcgcgtag aagctagtca agcctacgga
    18241 gcaaagctag aagagttttt tggtttacca gtagactatc aggatgaacg cttgacaaca
    18301 gtggctgctg agcgcatgtt gattgaacaa gcagatatca gtcgcaataa gcgcaagaaa
    18361 gtcattgata agttagcagc tcagctgatt ttacaaaatt atttagatag aaaattttaa
    18421 tataaaggag aggctatgtc acacgatcat aaccacgacc acgaagaacg tgaactaatc
    18481 acactagtag atgagcaagg aaatgaaacc ttgtttgaaa tccttttgac aattgatgga
    18541 aaagaagaat ttggtaaaaa ctatgttctt ctagtaccag ttaacgcaga agaagacgaa
    18601 gacggacaag ttgaaatcca agcttactca ttcattgaaa atgaagatgg aacagaaggc
    18661 gaattgcaac caatcccaga agactcagaa gacgaatgga acatgattga agaagtcttc
    18721 aacagcttta tggaggagtg atacagtctg ggagactgta tcagcccaac tccagaaatg
    18781 gggaagagtt ggaacatcta gtggatgttt ttagcccata ttcacattca tagtagctag
    18841 ttattagcta accaggtaag aggtcgggac gaaaatcctg gccttgtttt tattagtaac
    18901 catactttaa tgcgataatt gattggactt ttgtcaagga gatgtgtatg tttgaagtag
    18961 aagaatggct ccatagtcgg attggtttga attttcgatc aggtttgggc cgaatgcagc
    19021 aagcggtgga tttgttagga aatcctgagc agtcttaccc tattatccac gtaacaggga
    19081 ctaatgggaa aggatctacc attgctttta tgagggaatt atttatgggg catggcaaaa
    19141 aagttgcgac ctttacctcc cctcatatcg tctctatcaa tgaccgaatc tgcattaatg
    19201 ggcaacctat agcagacgca gactttatcc gtttgactga tcaggtcaag gagatggaga
    19261 aaacgcttct gcaaactcct gcccagttgt ccttttttga attgctgacc ttagttgctt
    19321 ttctttattt tagggagcaa gaggtggatt tggttttatt agaagtggga attggtggct
    19381 tacttgacac gaccaatgtg gtaactggag agcttgctgt catcacctcc attgggcttg
    19441 accatcaaga aaccttgggt gatagtctag aagcaattgc agagcagaaa gctggtattt
    19501 tcaaggctgg taagaaggca gtgattgcga aattgcctcc agaagctagg cttgtctgtc
    19561 agaaaaaagc cgaatcttta gctgttaacc tttatcaggc aggtcaagat tttttaatgc
    19621 tgaatggtga tttttcaagc tctttactaa atctttcaca gctgaacata ggcttagaag
    19681 gagtctatca gcaggagaat gcagccttgg cgttgcaaac ttttcttctt tttatgggag
    19741 aaggaaagga agctgttgat gaacaggctg taagaaaggc cttggaacag acccattggg
    19801 ctggtcgctt ggagcgtatt cgcccacaga tttacttgga tggtgctcat aacctccctg
    19861 ccttgactcg cttggttgag tttatcaaag aaaaagagca ggaaggttat cgacctcaaa
    19921 tcctctttgg atccttgaaa cgtaaggatt atcaagggat gttgggttat ctgactgaaa
    19981 atttacctca agtggaactc aagatgactg gctttgacta tcaagggtct ttggacgaga
    20041 cagatgtaac aggttaccat gtaattccct cttaccgaga atttatcagc agtttcgaag
    20101 aaagggcaga cgctcaagac ttgctgttcg taacagggtc tctctatttt atctcagaag
    20161 tacggggcta cctgctggac cgtgagcaga taaattgacc cccttttttg aacttgttat
    20221 actaggggaa ttgctggtta gagaaaattt ctctaaattg gtagcagaaa ggaaatccat
    20281 catgaaatta aaaagattca cactttctct tgcttctcta gcaagtttta gtctcttagt
    20341 agcttgttca caaagagctc aacaggttca acagcctgtt gctcagcagc aggtccaaca
    20401 gcctgctcaa cagaatacca atactgcaaa tgcagggggt aaccaaaatc aagcggctcc
    20461 agtacaaaac caacctgttg ctcagccgac cgatattgat gggacttata ctggtcagga
    20521 tgacggagac cgtatcactt tagtggtaac tggaacgact ggtacatgga ctgagctcga
    20581 atctgacggg gatcagaaag tcaaacaggt tacatttgat tcagcaaatc aacgcatgat
    20641 tattggcgat gatgtcaaaa tttacactgt aaacggtaat caaatcgtcg tagatgatat
    20701 ggatagagac ccatcggacc aaatcgtttt aactaaataa gactatgcaa gtagaaaggg
    20761 ctggattttt cagccttttt acttttacct agaaaataat cataaatact tgccttctac
    20821 cgaatacctg cgttatacta gtatcaatta cttgttttta gcattcaaac tatcaaggag
    20881 gggatatgaa atataggaaa tttcaattat tgatgtccaa gtatggcttt agtctttcga
    20941 ttatgttact tgaactttgt cttgtttttg gtctctttct ttatttagga cgcatggctc
    21001 ccattttatg gattactgtc ctcattctac tgagtatcat cacaatcatt tcgatagtca
    21061 accgtaatac gactcctgag aataaggtaa cctggttgtt agtagccttt gtgccagtat
    21121 ttggtccctt gctctatctg atgtttggtg aaaggcgatt gtccaaaaaa gaaatcaaac
    21181 aactgaagaa gctaggctct atgcatttcc aagaagcaaa tagccagcta ctaaaagaga
    21241 aattaaaaga aagtgacaag gcagcttatg gagtcatcaa gtccttattg agtatggata
    21301 ccaatgctga catctatgat caaactgcct ctacattttt tcctaacgga gaagctatgt
    21361 ggaaaaagat ggtagaagat cttaaaaagg ctgagaaatt tattttcttg gaatattaca
    21421 ttatagaaga aggtttgatg tggaatcgca tactagatat actagagcaa aaggtagctc
    21481 agggtgtaga ggttaagatg ctctatgatg atatcggctg tatggctact ttaacaggag
    21541 attatgcaca tcgacttcgt cagctgggca tcgaggccca taaattcaat aaagttattc
    21601 ctcgtttgac agtggcttat aataacagag atcatagaaa aatattgatt gttgatggtc
    21661 agatagccta tactggtggg gtcaatctgg cagatgagta cattaaccac gtcgagagat
    21721 ttggttattg gaaggatagt ggaattcgct tagacggact agcagtaaaa gccctgacac
    21781 gcttattttt gaccacttgg tacattaatc gaggagaaat tagtgatttt gatcaatatc
    21841 atttagaaaa tcattctatc ccgagtgacg gtttaaccat tccatacgga agtggaccca
    21901 agccaatttt tcgagcgcag gtagggaaaa aagtttatca gagtttaatc aatcaagcaa
    21961 cggaatcggt ctatattacg acaccttatt tgattataga ttatgattta acagagacaa
    22021 tcaaaaatgc agctatgaga ggggtcgatg ttcgaattat caccccttac ataccagata
    22081 agaagttcat tcagttagtc acgagaggag cttatcccga ccttctttct gctggtgttc
    22141 ggatttatga gtatagtcca ggttttattc atagtaagca gatgttggta gacgaagatt
    22201 ttgcggtggt ggggacaatc aatctcgact accggagctt ggtacaccat tatgaaaatg
    22261 cagtcttact ctataaaact ccttctataa gggaaatcgc ccgagatttt cgaaatatat
    22321 ttgcagattc tcaggaagtc tatcctcatt ctatcaaaac gagctggtat caaaagcttg
    22381 taaaagaaat cgcccagcta ttcgccccta tcttataaga aatctagaac ttaggataca
    22441 aaccagacct atttttcttg tcttttacga atagaaagaa agaggagggg aattcatgct
    22501 aactaaggaa gaagtgaatg atatgattga gttcaaacag acacatttac accgctttcg
    22561 ggaaattgaa acctttgtga aactatgcaa aaaatcactc aaacagccat cgcaagctga
    22621 caatcctagg actttttgat atactaagac agataaattg aacaggagaa acgatatgag
    22681 tgtgtatggt agagtagaag aagttcataa ggagaatcgt gaacctctag aataccaaat
    22741 cgaacaagaa tcgcatcatc gtgaatcaag tcgtcttcct ttggtgaaaa ttttactatg
    22801 gagtacgctt gtaacgggga taactctagg agtaccgcta ttactcgact taatgagtgc
    22861 acaagaagtg caggattttt atgcaggttg ggcgcttcat cagacaggga agatttacag
    22921 cgactattat ggaagtcaag gtttgcttta ttatttgctg acttacgtga gtcagggcgg
    22981 atttttcttt gccatctttg agtggttagc cttggtagca ggaggatttt tcctttttag
    23041 atcagcggac accttgacag agcaaggaga ccaagctgga catctggtga ctatttttta
    23101 catgctagtt acaggtcttg cttttggtgg aggttatgcg actcttttag cgcttccttt
    23161 cttattcgca gcctttagtt tagttgcggc ttacctaagc aatccaagcc atgataaggg
    23221 atttgtacgg attgggctag ctttggcagg cggatttttc tttgctccct tatcatcgct
    23281 cctgtttatt gctgtagtga gtttaggctt gttggtcttt aaccttgggc atagacgctt
    23341 tgcgcatggg ttttatcagt ttcttgcagt ggctttaggt ttttcacttg tcttttatcc
    23401 aactgcctac tatagtgctg caacaggaag ttttggggat gcgattagtg gtattcgtta
    23461 tcctattgac agtattcgct ttgattttac ttctaaaatt ttagagaata tgttttttta
    23521 tggcttgttg tcccttggtt tgggatttgt ggtttgtatc tttttaggtc tctttcaatc
    23581 caaacccttt aaactatacg tcatttcggt tcctgcaagt cttgtggtaa ttttaggttt
    23641 gattttactt ttcttttcac aagagcctct gcacgcttct tatttgatgg tcgtcttccc
    23701 tgttttccta cttttattgg taaccaatat taagagtcaa cagagggggc gtagtgctag
    23761 aagaagccga agagaaacgc cagttagcct atggagtcgt ttcttcaaag gaaatctata
    23821 tctgctagtt tttgggtttg tctatctttt gtttgttcct tttttgatga agtttgtcct
    23881 ttatccagta ccttatcaag aacgtaatcg tcttgctgat ttggtaaaag aggagacaaa
    23941 tacggaagat gctatctatg catgggatga tactgcgact ctttatcgta agagtgagcg
    24001 cttgtcgcca tcggcgattt tgtccccgtt gcactataca gcaactgagg aaaatcgtaa
    24061 taagttactt aatgacttga aagaaaaaca acctaaggtg attgtggtaa atgataaggt
    24121 ggtagtctgg tctgaagtgg aaacactctt aaaagaaaat taccaacaag taaagactga
    24181 ttactcagag tttaaagtct ataaaattaa ataaccaaat caatatcttg tgtattttta
    24241 aaaaatttag gatttttaac acaagatatt gatttttctt tttagagtgg tataatactt
    24301 tttagaaaga acattttaga aaagagcatg catatgattg cactagaaga aaaaattaca
    24361 attttgccaa ctctcttcgt cgagaaacga gatgggagac gtgttgtatt tgatgtggac
    24421 aagattgaca aggctctcca caaggcggct gacaaggtta tggatgtgac acccctggtt
    24481 gaaaaacgcc tcaatgatct gactgagcga attattacag aaattcatag tcgctttcca
    24541 cagggaatta agatttacga aattcaaaat atcgtagaac atgaactcct tgaagccaaa
    24601 gaatatgcgc tggctgagga gtatattact tatcggacac agagggattt tgagcgctca
    24661 aaagcgacgg atatcaactt tagtattcat aaacttctca acaaagacca gacagttgtc
    24721 aatgaaaacg ctaataaaga cagtgatgtc tttaacactc agcgtgattt gacagcaggg
    24781 attgttggga aatcaatcgg actgcaaatg cttcctaagc acgtagccaa tgcccaccaa
    24841 aagggggata tccactatca cgatttggac tacagtccct atacccctat gaccaactgc
    24901 tgtttgattg attttaaggg tatgttggaa aatggtttta agattggaaa tgcagaggta
    24961 gagagtccca agtctatcca gactgcgaca gcacagattt ctcaaatcat tgccaacgtt
    25021 gcttctagcc agtacggtgg ctgttcagct gaccgtatcg atgaaatttt ggcgccttat
    25081 gcagagaaga attatcaaaa acatctcaaa gatgcagaag agtgggtatt gcctgaaaaa
    25141 caggaagatt acgcttggaa gaaagcgcaa aaggacatct acgatgccat gcaatctctt
    25201 gagtatgaaa tcaatactct cttcacttca aatggacaaa caccttttac ttcgttaggt
    25261 tttggtctgg gaaccagtcg ttttgaacga gaaattcaaa aagctatttt aaacattcgc
    25321 atcaagggtc ttggttcaga acaccgtacg gctatctttc ctaaacttat ctttacgctt
    25381 aaaagaggcc tcaacttaga ggaaggaact cccaactatg acatcaagca gttggctcta
    25441 gagtgtgcaa ccaagcggat gtatccagac gtcttgtctt atgataagat tgttgatttg
    25501 acaggttctt tcaaggtgcc tatgggctgc cgttctttcc ttcaagggtg gaaggatgaa
    25561 aatggtgtag aagtcaattc aggtcgcatg aatctgggtg ttgtgacggt taatctgcct
    25621 cgtattgctc ttgagtctga aggtgatatg aataagttct gggaaatctt caacgagcga
    25681 atgaatatcg cagaagatgc tcttgtttac cgtgtcgaac gcactaaaga ggcgacacca
    25741 gcgaatgctc ctattcttta tcagtacggt gcttttggcc atcgtctagg taaagaagaa
    25801 agtgttgacc agctctttaa gaatcgtcgt gcgaccgttt cgctgggcta tatcggcttg
    25861 tatgaagtag cgacagtttt ctttggtaac agctgggaaa gtaatccaga tgctaaggaa
    25921 ttcacgctag acatcattca cgatatgaaa cgccgtgtag aagagtggtc agaccaatat
    25981 ggctaccatt tctctatcta ctcaacacca tccgaaagtc tgacagaccg tttctgccga
    26041 ctagatatag acaagtttgg ctctattcct gatatcacag acaaggaata ctacaccaac
    26101 tctttccact acgatgttcg taaaaatcca acaccgtttg aaaaattgga ctttgagaaa
    26161 gtctatccag aagcaggtgc gtcaggtggt ttcatccatt attgtgagta tccagtcctt
    26221 cagcaaaatc caaaggcctt ggaagctgtc tgggattatg cttatgaccg tgtaggctat
    26281 ctaggcacca atactccgat tgaccgttgc tacaagtgtg actttgaagg ggattttgaa
    26341 ccaactgaga gagggtttgc ttgtccaaac tgtggcaata gcgaccctaa aacagtagat
    26401 gtggtgaagc gaacttgtgg ctacctaggt aatcctcaag caagaccgat ggtcaacggg
    26461 cgtcacaagg aaatcgctgc gcgtgtcaaa catatgaatg gttcaacgat taaaatagct
    26521 gggcatcaag taacaaatta gaaagaaatg aaatgggaaa atatcaacta gacgataagg
    26581 ggcgcgcaca agtgacccgt tatcacgaga aacactctaa aggtggagct ggtaagaaag
    26641 aacgcttgct tagcttcaga gaacaatttt taaacaagaa caagaaaaaa taaaagtgag
    26701 agccagctct cgcttttctc atagtgggag gtaaggatgg aattacgcag accaagatta
    26761 gcggataaga aagctgtttt agatatgatg acagagtttg aaaaatttca gtcgcctcac
    26821 gacggcggtt tctgggatac agagaacttt gtgtatgaag actggttaga aagcaatcag
    26881 gaacaggaaa tggggattaa tctgcctgaa ggatgggttc ctgcaattca gttagtggct
    26941 ttttctgaga aaggtcaagc agttggattt cttaatctcc ggttgcgcct cagtaacttt
    27001 ttactagaag aaggtgggca cattggctac tccatccgtc catctgaaag aggcaagggt
    27061 tatgcaaaag agactctccg tcagggcttg caagttgcta aggaaaagaa catcaagaaa
    27121 gctctggtga cctgtagcgt gaataatcct gctagcagag cagtcattct agcaaatggt
    27181 ggaatatttg aggatgctcg caatggagtc gagcgttatt ggatagaggt agcgaatgaa
    27241 taatccaaaa ccacaagaat ggaaaagcga ggaacttagt caaggtcgta tcattgacta
    27301 caaggccttt aactttgttg atggcgaagg cgtgcgtaac tctctctatg tatcaggctg
    27361 tatgtttcac tgcgagggat gttataatgt tgcgacttgg tcttttaatg ctggcattcc
    27421 ctatacagca gaattagaag agcagatcat ggcagacctt gcccaaccct atgttcaagg
    27481 cttgactttg ctgggagggg agccttttct caatactggc attctcttgc ctctagttaa
    27541 acgcatccga aaggaattgc cagacaagga catttggtcc tggacgggct acacttggga
    27601 agaaatgatg ttggaaactc cagataaact ggaactctta tcattgattg acattcttgt
    27661 cgatggacga tacgatcgaa ctaagagaaa tctcatgctc cagtttcgag gttcgtccaa
    27721 ccaacgaatt atcgatgtgc aaaaatcgct caaaagtggg caagtagtga tttgggacaa
    27781 gctcaatgac ggaaaagaaa gctatgaaca ggtgaagaga gaatgaagaa aaaagactta
    27841 gtagaccaac tagtctcaga gatcgagacg gggaaagtca ggacactggg aatatacggt
    27901 catggagctt caggtaaatc aacctttgca caggaattgt accaagcttt agattctact
    27961 acagtaaatt tgctagagac agatccttat atcacctcag gacgccatct ggtagtaccc
    28021 aaggacgcgc cgaatcaaaa ggtgacagcc agtctgccag tggcgcatga actggagagt
    28081 ttgcagagag atatccttgc cttgcaggcg ggtatggatg tcttgacaat tgaagaacct
    28141 tggaaggcta gtgaggtctt gtctggagcc aaaccaattt tgattgtcga agggatgtct
    28201 gttggctttc tacccaagga actctttgaa aaaaccatct gtttctacac ggatgaggag
    28261 accgaattaa agcgacgcct tgctagagat acgactgtga gaaatcgcga tgcatccttt
    28321 atattggcta gccatcagat gagacgggag cagtatctgc gatactataa agaaactgag
    28381 tctaaggcgg atatcttagt ggaccaatca gaagataaat ttgatgtcaa gaggacttaa
    28441 cttatgtata gaagaaatat agaagaaaat ccctaatttt agaaagaaaa agtaggaaaa
    28501 cagtttcatc ccaaaaaaac gaaaaaacct aacaaatccc ttgcaatcgc aggggatttg
    28561 tgttattcta ttatggtgct gtaaattaca gccttagttt tgatgcaaga ggttgcgaca
    28621 cgctcggttg cattgccacg caacaccgcg tcggttttct tgtggagcta gcctattatc
    28681 ttaaatagac gaaaaggaga aaaagatggc aaacaaaaaa atccgtatcc gtttgaaagc
    28741 ttacgaacac cgtacgcttg acacagcggc tgcaaaaatc gtagaatcag ctactcgtac
    28801 aggtgcacaa gttgcgggtc caatcccact tccaactgaa cgtagcctct acacaatcat
    28861 tcgtgcgact cacaaataca aagactctcg cgaacaattt gaaatgcgta cacacaaacg
    28921 tttgatcgat atcgttaacc caactcaaaa aacagttgat gccttgatga aattggatct
    28981 tccaagtggt gtaaacgtag aaatcaaact ttaatctaaa atataaaaga gcagaggctg
    29041 gtgtttcaat ctaattgaac acgggctaaa ctcggtgtga aaaagataaa cttcctagtg
    29101 tctgctagac actgcgtcag tttcctattt tcactttgag tttgacgccc tttgtatctt
    29161 agacttgagc ataaaaaacg ctcgttaaaa actttttgaa taaaaaatat agaaaaggaa
    29221 ctattttctc atgacaaaag gaatcttagg gaaaaaagtg ggaatgactc aaatcttcac
    29281 tgaagctggc gaattgatcc ctgtaacagt tattgaagca actccaaacg ttgttcttca
    29341 agttaaaact gttgaaacag acggatacaa cgctatccaa gttggtttcg atgacaaacg
    29401 cgaagtattg agcaacaaac ctgctaaagg acatgtagcg aaagctaaca cggctcctaa
    29461 gcgcttcatt cgtgaattca aaaacgttga aggcttggaa gttggtgctg aaattacagt
    29521 tgaaacattc gcagctggag acgttgttga cgtaactggt acttctaaag gtaaaggttt
    29581 ccaaggtgtt atcaaacgcc acggacaatc acgtggacca atggctcacg gttctcgtta
    29641 ccaccgtcgt ccaggttcta tgggacctgt tgcacctaac cgcgtattca aaggtaaaaa
    29701 ccttgcaggg cgtatgggtg gcgaccgcgt aacaattcaa aaccttgaag ttgtacaagt
    29761 tgttccagaa aagaacgtta tccttatcaa aggtaacgta ccaggtgcta agaaatctct
    29821 tatcactatc aaatcagcag ttaaagctgg taaataataa agaaagggga aatcagtcac
    29881 aatggcaaac gtaacattat ttgaccaaac tggtaaagaa gctggccaag ttgttcttag
    29941 cgatgcagta tttggtatcg aaccaaatga atcagttgtg tttgatgtaa tcatcagcca
    30001 acgcgcaagc cttcgtcaag gaacacacgc tgttaaaaac cgctctgcag tatcaggtgg
    30061 tggacgcaaa ccatggcgtc aaaaaggaac tggacgtgct cgtcaaggtt ctatccgctc
    30121 accacaatgg cgtggtggtg gtgttgtctt cggaccaact ccacgttcat acggctacaa
    30181 acttccacaa aaagttcgtc gcctagctct taaatcagtt tactctgaaa aagttgctga
    30241 aaacaaattc gtagctgtag acgctctttc atttacagct ccaaaaactg ctgaatttgc
    30301 aaaagttctt gcagcattga gcatcgattc taaagttctt gttatccttg aagaaggaaa
    30361 tgaattcgca gctctttcag ctcgtaacct tccaaacgtg aaagttgcaa ctgctacaac
    30421 tgcaagtgtt cttgacatcg caaatagcga caaacttctt gtcacacaag cagctatctc
    30481 taaaatcgag gaggttcttg cataatgaat ttgtatgatg ttatcaaaaa acctgtcatc
    30541 actgaaagct caatggctca acttgaagca gggaaatatg tatttgaagt tgacactcgt
    30601 gcacacaaac ttttgatcaa gcaagctgtt gaagctgctt tcgaaggtgt taaagttgcc
    30661 aatgttaaca caatcaacgt aaaaccaaaa gctaaacgtg ttggacgtta cactggtttt
    30721 actaacaaaa ctaaaaaagc tatcatcaca cttacagctg attctaaagc aatcgagttg
    30781 tttgctgctg aagctgaata atctaaggag gaaatatcgt gggaattcgt gtttataaac
    30841 caacaacaaa cggtcgccgt aatatgactt ctttggattt cgctgaaatc acaacaagca
    30901 ctcctgaaaa atcattgctt gttgcattga agagcaaggc tggtcgtaac aacaacggtc
    30961 gtatcacagt tcgtcaccaa ggtggtggac acaaacgttt ctaccgtttg gttgacttca
    31021 aacgtaataa agacaacgtt gaagcagttg ttaaaacaat cgagtacgat ccaaaccgtt
    31081 ctgcaaacat cgctcttgta cactacactg acggtgtgaa agcatacatc atcgctccaa
    31141 aaggtcttga agtaggtcaa cgtatcgttt caggtccaga agcagatatc aaagtcggaa
    31201 acgctcttcc acttgctaac atcccagttg gtactttgat tcacaacatc gagttgaaac
    31261 caggtcgtgg tggtgaattg gtacgtgctg ctggtgcatc tgctcaagta ttgggttctg
    31321 aaggtaaata tgttcttgtt cgtcttcaat caggtgaagt tcgtatgatt cttggaactt
    31381 gccgtgctac agttggtgtt gtcggaaacg aacaacatgg acttgtaaac cttggtaaag
    31441 caggacgtag ccgttggaaa ggtatccgcc caacagttcg tggttctgta atgaacccta
    31501 acgatcaccc acacggtggt ggtgaaggta aagcaccagt tggtcgtaaa gcaccatcta
    31561 ctccatgggg caaacctgct cttggtctta aaactcgtaa caagaaagcg aaatctgaca
    31621 aacttatcgt tcgtcgtcgc aacgagaaat aatattaaac tagtcgctta agcaactagt
    31681 aaatccgcca gctcggtagc gctccatagg agtgcaagcc gctgtggtac aacatttaaa
    31741 ggagaaaata taaaaatggg acgcagtctt aaaaaaggac ctttcgtcga tgagcatttg
    31801 atgaaaaaag ttgaagctca agctaacgac gaaaagaaaa aagttattaa aacttggtca
    31861 cgtcgttcaa cgatcttccc aagtttcatt ggttacacta ttgcagttta tgacggacgt
    31921 aaacacgtac ctgtttacat ccaagaagac atggtaggcc acaaacttgg tgaatttgca
    31981 ccaactcgta cttacaaagg tcacgctgca gacgacaaga aaacacgtag aaaataagga
    32041 gaacataaat ggcagaaatt acttcagcta aagcaatggc tcgtacagta cgtgtttcac
    32101 ctcgtaaatc acgtcttgtt cttgataaca tccgtggtaa aagcgtagcc gatgcaatcg
    32161 caatcttgac attcactcca aacaaagctg ctgaaatcat cttgaaagtt ttgaactcag
    32221 ctgtagctaa cgctgaaaac aactttggtt tggataaagc taacttggta gtatctgaag
    32281 cattcgcaaa cgaaggacca actatgaaac gtttccgtcc acgtgcgaaa ggttcagctt
    32341 caccaatcaa caaacgtaca gctcacatca ctgtagctgt tgcagaaaaa taaggaggta
    32401 aaatcgtggg tcaaaaagta catccaattg gtatgcgtgt cggcatcatc cgtgattggg
    32461 atgccaaatg gtatgctgaa aaagaatacg cggattacct tcatgaagat cttgcaatcc
    32521 gtaaattcgt tcaaaaagaa cttgctgacg cagcagtttc aactattgaa atcgaacgcg
    32581 cagtaaacaa agttaacgtt tcacttcaca ctgctaaacc aggtatggtt atcggtaaag
    32641 gtggtgctaa cgttgatgca ctccgtgcaa aacttaacaa attgactgga aaacaagtac
    32701 acatcaacat catcgaaatc aaacaacctg atttggatgc tcaccttgta ggtgaaggaa
    32761 ttgctcgtca attggagcaa cgtgttgctt tccgtcgtgc acaaaaacaa gcaatccaac
    32821 gtgcaatgcg tgctggagct aaaggaatca aaactcaagt atcaggtcgt ttgaacggtg
    32881 cagatatcgc ccgtgctgaa ggatactctg aaggaactgt tccgcttcac acacttcgtg
    32941 cagatatcga ttacgcttgg gaagaagcag atactacata cggtaaactt ggtgttaaag
    33001 tatggatcta ccgtggtgaa gttcttccag ctcgtaaaaa cactaaagga ggtaaataac
    33061 caatgttagt acctaaacgt gttaaacacc gtcgtgaatt ccgtggaaaa atgcgcggtg
    33121 aagcaaaagg tggaaaagaa gtagcattcg gtgaatacgg tcttcaagct acaactagcc
    33181 actggatcac taaccgccaa atcgaagctg ctcgtatcgc catgactcgt tacatgaaac
    33241 gtggtggtaa agtttggatt aaaatcttcc cacacaaatc atacactgct aaagctatcg
    33301 gtgtgcgtat gggatctggt aaaggggcac ctgaaggttg ggtagcacca gttaaacgtg
    33361 gtaaagtgat gttcgaaatc gctggtgtat ctgaagagat tgcacgtgaa gcgcttcgac
    33421 ttgctagcca caaattgcca gttaaatgta aattcgtaaa acgtgaagca gaataaggag
    33481 aaggcatgaa acttaatgaa gtaaaagaat ttgttaaaga acttcgtggt ctttctcaag
    33541 aagaactcgc gaagcgcgaa aacgaattga aaaaagaatt gtttgaactt cgtttccaag
    33601 ctgctactgg tcaattggaa caaacagctc gcttgaaaga agttaaaaaa caaatcgctc
    33661 gtatcaaaac agttcaatct gaagcgaaat aatagactag ggaaggagaa atttcaatgg
    33721 aacgcaataa tcgtaaagtt cttgttggac gtgttgtatc tgacaaaatg gacaagacaa
    33781 tcacagttgt agttgaaaca aaacgtaacc acccagtcta tggtaaacgt attaactact
    33841 ctaaaaaata caaagctcat gatgaaaaca atgttgccaa agaaggtgat atcgtacgta
    33901 tcatggaaac tcgcccgctt tcagctacaa aacgtttccg tcttgtagaa gttgttgaag
    33961 aagcggtcat catctaatca aacctgaaag gagaaaactg aaatgattca aacagaaact
    34021 cgtttgaaag tcgcagacaa cagcggtgct cgcgaaatct tgactatcaa agttcttggt
    34081 ggttcaggac gtaaatttgc aaacatcggt gatgttatcg tggcgtctgt aaaacaagct
    34141 actcctggtg gtgcggttaa aaaaggtgac gttgttaaag cagttatcgt tcgtactaaa
    34201 tcaggtgctc gtcgtgctga tggttcatac atcaaatttg acgaaaacgc agcagttatc
    34261 atccgtgaag acaaaactcc tcgcggaaca cgtatctttg gcccagttgc acgtgaattg
    34321 cgtgaaggtg gtttcatgaa gatcgtgtca cttgctccag aagtacttta atttttagga
    34381 acaaactagt cccctagctt caagctaggg tgcccttatg ggcgtaagaa aaatcaagga
    34441 gaaacctaat gtttgtaaaa aaaggcgaca aagttcgcgt aatcgctggt aaagataagg
    34501 gaacagaagc tgttgtcctt actgcccttc caaaagtaaa caaagttatc gttgaaggtg
    34561 ttaacattgt taagaaacac caacgtccaa ctaacgagct tcctcaaggt ggtatcatcg
    34621 agaaagaagc agctatccac gtatcaaatg ttcaagtttt ggacaaaaat ggtgtagctg
    34681 gtcgtgttgg ttacaaattt gtagacggta aaaaagttcg ctacaacaaa aaatcaggcg
    34741 aagtgcttga ttaatcacga aggaaaggag aagtataatg gcaaatcgtt taaaagaaaa
    34801 atatcttaat gaagtagttc ctgctttgac agaacaattc aactactcat cagtgatggc
    34861 tgtgcctaaa gtagataaga ttgttttgaa catgggtgtt ggtgaagctg tatcaaacgc
    34921 taaaagcctt gaaaaagctg ctgaagaatt ggcacttatc tcaggtcaaa aaccacttat
    34981 cactaaagct aaaaaatcaa tcgccggctt ccgtcttcgt gaaggtgttg cgatcggtgc
    35041 aaaagttacc cttcgtggtg aacgtatgta cgaattcttg gataaattgg tatcagtttc
    35101 acttccacgt gtacgtgact tccacggtgt cccaacaaaa tcatttgatg gacgcgggaa
    35161 ctacacactt ggtgtgaaag aacaattaat cttcccagaa atcaacttcg atgacgttga
    35221 caaaactcgt ggtcttgaca tcgttatcgt aacaactgct aacactgacg aagagtcacg
    35281 tgcattgctt acaggccttg gaatgccttt tgcaaaataa tataggaggt aaatctaatg
    35341 gctaaaaaat caatggtagc tagagaggct aaacgccaaa aaattgttga ccgttatgct
    35401 gaaaaacgtg ctgcattaaa ggcggcaggg gactacgaag gtttatctaa attacctcgc
    35461 aacgcctcac cgactcgttt acataatcgt tgtagggtta cggggcgccc acattcagtt
    35521 taccgcaaat ttggtctgag tcgtatcgct tttcgcgaac ttgcgcataa aggtcaaatt
    35581 cctggtgtaa caaaagcatc ttggtaattt aagatatcaa gagcgtcaaa actccaagta
    35641 aaaataggaa acttgacgaa gaaactaaag tttctaggaa agtttatctt tttcacacag
    35701 agtttagccc gtgttcaatt gggcttgcca atttgaacac gagctacagc tttggcaaaa
    35761 aagaccaatt tgctttggag cattgcttct gcattaaatt gtctattttt gctcgtgctg
    35821 ttacgctctt tgtatcatgt attaactagc aagtgcaact tgcaaactac tagtaagagg
    35881 agaaaaacaa aatggttatg actgacccaa tcgcagactt cctaactcgt attcgtaatg
    35941 ctaaccaagc taaacacgaa gtacttgaag tacctgcatc aaacatcaaa aaagggattg
    36001 ctgaaatcct taaacgcgaa ggttttgtaa aaaacgttga aatcattgaa gatgacaaac
    36061 aaggcgtcat ccgtgtattt cttaaatacg gaccaaatgg tgagaaagtt atcactaact
    36121 tgaaacgtgt ttctaaacca ggacttcgtg tctacaaaaa acgtgaagac cttccaaaag
    36181 ttcttaacgg acttggaatt gccatccttt caacttctga aggtttgctt actgataaag
    36241 aagcacgcca aaagaatgtt ggtggtgagg ttatcgctta cgtttggtaa aatcaagata
    36301 caaagctcgt aaagaacaaa gcaaaattag gaagttggag aagtttgttt acaaacaagc
    36361 caacttatct attttgcaca gttcttagag cgtgttcagt tcagctcttg aactaaataa
    36421 gtatctgaac cccgtgaaaa ctggccgttc tggcctgaca atctaacagg agaaaataaa
    36481 catgtcacgt attggtaata aagttatcgt gttgcctgct ggtgttgaac tcgctaacaa
    36541 tgacaacgtt gtaactgtaa aaggacctaa aggagaactt actcgtgagt tctcaaaaga
    36601 tattgaaatc cgtgtggaag gtactgaaat aactcttcac cgtccaaacg attcaaaaga
    36661 aatgaaaact atccacggaa ctactcgtgc ccttttgaac aacatggttg ttggtgtatc
    36721 agaaggattc aagaaagaac ttgaaatgcg tggggttggt taccgtgcac agcttcaagg
    36781 atctaaactt gttttggctg ttggtaaatc tcatccagac gaagttgaag ctccagaagg
    36841 aattactttt gaacttccaa acccaacaac aatcgttgtt agcggaattt caaaagaagt
    36901 agttggtcaa acagctgctt acgtacgtag ccttcgttca ccagaaccat ataaaggtaa
    36961 aggtatccgt tacgttggtg aattcgttcg tcgtaaagaa ggtaaaacag gtaaataatg
    37021 ttgagtggtt gatcatcaac caccaaccta ttttccaact ttgtgcatag cacacgattt
    37081 aaaactaaag aggtgaaaac tgtgatttca aaaccagata aaaacaaact ccgccaaaaa
    37141 cgccaccgtc gcgttcgcgg aaaactctct ggaactgctg atcgcccacg tttgaacgta
    37201 ttccgttcta atacaggcat ctacgctcaa gtgattgatg acgtagcggg tgtaacgctc
    37261 gcaagtgctt caactcttga taaagaagtt tcaaaaggaa ctaaaactga acaagccgtt
    37321 gctgtcggta aactcgttgc agaacgtgca aacgctaaag gtatttcaga agtggtgttc
    37381 gaccgcggtg gatatctata tcacggacgt gtgaaagctt tggctgatgc agctcgtgaa
    37441 aacggattga aattctaata ggaggacact agaaaatggc atttaaagac aatgcagttg
    37501 aattagaaga acgcgtagtt gctgtcaacc gtgttacaaa agttgttaaa ggtggacgtc
    37561 gtcttcgttt cgcagctctt gttgttgttg gtgaccacaa tggtcgcgta ggatttggta
    37621 ctggtaaagc tcaagaagtt ccagaagcaa tccgtaaagc agtagatgat gctaagaaaa
    37681 acttgatcga agttcctatg gttggaacaa caatcccaca cgaagttctt tcagaattcg
    37741 gtggagctaa agtattgttg aaacctgctg tagaaggttc tggagttgcc gctggtggtg
    37801 cagttcgtgc cgttgtggaa ttggcaggtg tggcagatat tacatctaaa tcacttggtt
    37861 ctaacactcc aatcaacatt gttcgtgcaa ctgttgaagg tttgaaacaa ttgaaacgcg
    37921 ctgaagaaat tgctgccctt cgtggtattt cagtttctga tttggcataa gaaaggggat
    37981 aaaatggctc aaattaaaat tactttgact aagtctccaa tcggacgcat tccatcacaa
    38041 cgtaaaactg ttgtagcact tggacttggc aaattgaaca gctctgttat taaagaagat
    38101 aacgctgcta tccgtggtat gatcacagca gtatctcact tagtaacagt tgaagaagta
    38161 aactaatgaa gttttagggg atgtgcactg taccatcccc taaaactaga tatagtcatc
    38221 tatgatgaca tcgtataggc gagttgatgg gggagacaac cttttctccc ttatcggcgc
    38281 tagcatttta caaaagagga gaaaataaaa atgaaacttc atgaattgaa acctgcagaa
    38341 ggttctcgta aagtacgtaa ccgcgttggt cgtggtactt catcaggtaa cggtaaaaca
    38401 tctggtcgtg gtcaaaaagg tcaaaaagct cgtagcggtg gcggagttcg ccttggtttt
    38461 gaaggtggac aaactccatt gttccgtcgt cttccaaaac gtggattcac taacatcaac
    38521 gctaaagaat acgcaattgt gaaccttgac caattgaacg tctttgaaga tggtgctgaa
    38581 gtaactccag ttgttcttat cgaagcagga attgttaaag ctgaaaagtc aggtattaaa
    38641 attcttggta acggtgagtt gactaagaaa ttgactgtga aagcagctaa attctctaaa
    38701 tcagctgaag aagctatcac tgctaaaggt ggttcagtag aagtcatcta agagaggtga
    38761 cctatgtttt ttaaattatt aagagaagct cttaaagtca agcaggttcg atcaaaaatt
    38821 ttatttacaa tttttatcgt tttggtcttt cgtatcggaa ctagcattac agttcctggt
    38881 gtgaatgcca atagcttgaa tgctttaagt ggattatcct tcttaaacat gttgagcttg
    38941 gtgtcgggga atgccctaaa aaacttttcg atttttgccc taggagttag tccctatatc
    39001 accgcttcta ttgttgtcca actcttgcaa atggatattt tacccaagtt tgtagagtgg
    39061 ggtaaacaag gggaagtagg tcgaagaaaa ttgaatcaag ctactcgtta tattgctcta
    39121 gttctcgctt ttgtgcaatc tatcgggatt acagctggtt ttaatacctt ggctggagct
    39181 caattgatta aaactgcttt aactccacaa gtttttctga caattggtat catcttaaca
    39241 gctggtagta tgattgtcac ttggttgggt gagcaaatta cagataaggg atacggaaac
    39301 ggtgtttcca tgattatctt tgccgggatt gtttcctcaa ttccagagat gattcagggc
    39361 atctatgtgg actactttgt gaacgtccca agtagccgta tcacttcatc tatcattttc
    39421 gtaatcattt tgattattac tgtattgttg attatttact ttacaactta tgttcaacaa
    39481 gcagaataca aaattccaat ccaatatact aaggttgcac aaggtgctcc atctagctct
    39541 taccttccgt taaaggtaaa ccctgctgga gttatccctg ttatctttgc cagttcgatt
    39601 actgcagcgc ctgcggctat tcttcagttt ttgagtgcca caggtcatga ttgggcttgg
    39661 gtaagggtag cacaagagat gttggcaact acttctccaa ctggtattgc catgtatgct
    39721 ttgttgatta ttctctttac attcttctat acgtttgtac agattaatcc tgaaaaagca
    39781 gcagagagcc tacaaaagag tggtgcctat atccatggag ttcgtcctgg taaaggtaca
    39841 gaagaatata tgtctaaact tcttcgtcgt cttgcaactg ttggttccct cttccttggt
    39901 gtgatttcca ttttaccgat tgcagctaaa gatgtatttg gtctttctga tgttgttgcc
    39961 tttggtggaa caagtctctt gatcattatc tctacaggta tcgaaggaat caagcaattg
    40021 gaaggttacc tattgaaacg taagtatgtt ggtttcatgg acagaacaga ataaaagtat
    40081 ttactgaatc agtaaatgct gagggagtgg aggtttaaac tctgacattt gtgagagttt
    40141 ggtctcccct cttctatttt gtttttaaat aggggtgaaa agactttttg cttctattta
    40201 aaaataaaat aaggagatca aatcatgaat cttttgatta tgggcttacc tggtgcaggt
    40261 aagggaactc aagcagcaaa aatcgtagaa caattccatg ttgcacatat ctcaacaggt
    40321 gatatgttcc gcgctgcaat ggcaaatcaa actgaaatgg gtgttcttgc taagtcatat
    40381 attgacaagg gtgaattggt tcctgacgaa gttacaaatg gaatcgtaaa agaacgtctt
    40441 tcacaagatg atattaaaga aacaggattc ttattggatg gttacccacg tacaattgaa
    40501 caagctcagg ccttggacaa aacattggct gaacttggca ttgaactaga aggtgttatc
    40561 aatattgaag tgaaccctaa cagccttttg gaacgtttga gtggccgtat catccaccgc
    40621 gtaactggag aaactttcca caaggtcttt aacccaccag ttgactataa agaagaagat
    40681 tactaccaac gtgaagatga taagcctgag acagtaaaac gtcgtttgga tgttaatatt
    40741 gctcaaggag aaccaatcat tgctcactac cgtgccaaag gtttggttca tgacatcgaa
    40801 ggtaatcaag atatcaatga tgtcttctca gatattgaaa aagtattgac aaatttgaaa
    40861 taaagcgttt ttcacacttg ccaaaatccg ctacaaatgt tatactgaga tagtctgact
    40921 tataattgtt gtctctgtgt ctagaggcat cgaatcgaaa tttatggagg tgcttttgcg
    40981 tggcaaaaga cgatgtgatt gaagttgaag gcaaagtagt tgatacaatg ccgaatgcaa
    41041 tgtttacggt tgaacttgaa aatggacatc agattttagc aacagtttct ggtaaaattc
    41101 gtaaaaacta tattcgtatt ttagcgggag atcgtgttac tgtcgaaatg agtccatatg
    41161 acttgacacg tggacgtatc acttaccgct ttaaataatc gaaaaacttg gagggataag
    41221 aaatgaaagt aagaccatcg gtcaaaccaa tttgcgaata ctgtaaagtt attcgtcgta
    41281 atggtcgtgt tatggtaatt tgcccagcaa atccaaaaca caaacaacgt caaggataag
    41341 atagaaagga gaaaacatgg ctcgtattgc tggagttgat attccaaatg acaaacgcgt
    41401 agtaatctca ttgacttatg tttatggtat cggacttgca acatctaaga aaattttggc
    41461 tgctgctgga atctcagaag atgttcgtgt acgtgatctt acatcagatc aagaagatgc
    41521 tatccgtcgt gaagtggatg caatcaaagt tgaaggtgac cttcgtcgtg aagtaaactt
    41581 gaacatcaaa cgtttgatgg aaatcggttc ataccgtggt atccgtcacc gtcgtggact
    41641 tcctgtccgt ggacaaaaca ctaaaaacaa cgctcgcact cgtaaaggta aagctgttgc
    41701 gattgctggt aagaaaaaat aatataggag gtaaaagtct tggctaaacc aacacgtaaa
    41761 cgtcgtgtga aaaagaatat cgaatctggt attgctcata ttcacgctac atttaataac
    41821 actattgtta tgattactga tgtgcatggt aatgcaattg cttggtcatc agctggtgct
    41881 cttggtttca aaggttctcg taaatctaca ccattcgctg ctcaaatggc ttctgaagct
    41941 gctgctaaat ctgcacaaga acacggtctt aaatcagttg aagttactgt aaaaggtcca
    42001 ggttctggtc gtgagtcagc tattcgtgcg cttgctgccg ctggtcttga agtaacagca
    42061 attcgtgatg tgactccagt gccacacaat ggtgctcgtc ctccaaaacg tcgccgtgta
    42121 taatcatcgc attacactgc ttttcgttta agagggagta actaaatgat cgagtttgaa
    42181 aaaccaaata taacaaaaat tgatgaaaat aaagattatg gcaagtttgt aatcgaacca
    42241 cttgaacgtg gctacggtac aactcttggt aactctcttc gtcgtgtact tctagcttct
    42301 ctaccaggag cagctgtgac atctatcaac attgatggtg tgttacatga gtttgacaca
    42361 gttccaggtg ttcgtgaaga cgtgatgcaa atcattctga acattaaagg aattgcagtg
    42421 aaatcgtacg ttgaagacga aaaaatcatc gaactggatg ttgaaggtcc tgctgaaata
    42481 acagctggtg acattttgac agatagcgat attgaaattg taaatccaga tcattatctc
    42541 tttacaattg gtgaaggttc ttctctaaaa gcgactatga ctgttaacag tggtcgtgga
    42601 tatgtacctg ctgatgaaaa taaaaaggat aatgcaccag ttggaacact tgctgtagat
    42661 tctatttata caccagttac aaaagtcaac tatcaagtgg aacctgctcg tgtaggtagc
    42721 aatgatggtt tcgacaaatt aacccttgaa atcttgacaa atggaacaat tattccagaa
    42781 gatgctttag ggctttcagc acgtattttg acagaacatc ttgatttgtt tacaaatctt
    42841 actgagattg ctaagtcaac tgaagtgatg aaagaagctg atactgaatc tgacgaccgt
    42901 attttagatc gtacgattga ggaactggac ttgtctgtgc gttcatacaa ctgtttaaaa
    42961 cgtgccggta tcaatactgt gcatgatttg acagaaaaat ctgaagcaga gatgatgaaa
    43021 gtacgaaatc ttggacgcaa gagtttggaa gaagtgaaac tcaaactcat tgatttgggt
    43081 cttggattaa aagataaata aaggaggaat acatggctta ccgtaaacta ggacgcacta
    43141 gctcacaacg taaagcaatg cttcgcgatt tgacaactga ccttttgatc aacgaatcaa
    43201 tcgtgacaac tgaagctcgt gctaaagaaa tccgtaaaac tgttgaaaaa atgattactc
    43261 taggtaaacg tggtgatttg catgcacgtc gtcaagcagc tgctttcgta cgtaatgaaa
    43321 tcgcatctga aaactatgat gaagcaactg ataagtacac ttctactaca gcacttcaaa
    43381 aattgttctc agaaatcgca cctcgttatg ctgaacgtaa cggtggatac actcgtatcc
    43441 ttaaaactga accacgtcgt ggtgatgcag cgccaatggc gatcatcgaa ttagtataaa
    43501 atcatcaatt ttgttgagtg ttatgatgat ggagtcttgt gctcttagtc tagctctggt
    43561 ctaccgctag gatttcggtc ctagcgggaa cactcatcat aagttgggat agtagacgct
    43621 tgtttacgaa attgtttttt tcttaagaac aacttcgtaa gcaggcgttt ttgagtattt
    43681 tcgttagaat tatgctatac tatttgaaaa gaatcctgtt taatgttaag gtttcttatt
    43741 ttaagaagaa ttggagtttg cttatgaaag cgattataac tgttgttggt aaagataaat
    43801 ctggaattgt tgcaggtgtt tctggtaaaa ttgcagaatt aggattgaat attgacgata
    43861 tctctcaaac tgtcttggat gaatatttta cgatgatggc tattgtatct agtgatgaaa
    43921 agcaagattt tacctatctt cgtaatgaat ttgaagcttt tgggcaaact ttgaatgtaa
    43981 aaatcaatat tcagagtgca gcgattttcg aagctatgta taatatctag gaggttatca
    44041 tggatattag acaagttact gaaaccatcg ccatgattga ggagcaaaat ttcgatatca
    44101 gaaccattac catggggatt tctcttttgg actgtatcga tccagatatc aatcgtgctg
    44161 cggagaaaat ctatcaaaaa attacgacaa aggcggctaa tttagtagct gttggtgatg
    44221 aaattgcggc tgagttggga attcctatcg ttaataagcg tgtatcggtg acacctattt
    44281 ctctgattgg ggcagcgaca gatgcgacgg actacgtggt tctggcaaaa gcgcttgata
    44341 aggctgcgaa agagattggt gtggacttta ttggtggttt ttctgcctta gtacaaaaag
    44401 gttatcaaaa gggagatgag attctcatca attccattcc tcgcgctttg gctgagacgg
    44461 ataaggtctg ctcgtcagtc aatatcggct caaccaagtc tggtattaat atgacggctg
    44521 tggcagatat gggacgaatt atcaaggaaa cggcaaatct ttcagatatg ggagcggcca
    44581 agttggttgt attcgctaat gctgttgagg acaatccatt tatggcgggt gcctttcatg
    44641 gtgttgggga agcagatgtt atcatcaatg tcggagtttc tggtcctggt gtggtgaaac
    44701 gtgctttgga aaaagttcgt ggacagagct ttgatgtagt agccgaaaca gttaagaaaa
    44761 ctgcctttaa aatcactcgt atcggtcaat tggttggtca aatggccagt gagagactgg
    44821 gtgtggagtt tggtattgtg gacttgagtt tggcaccaac ccctgcggtt ggagactctg
    44881 tggcacgtgt ccttgaggaa atggggctag aaacagttgg cacgcatgga acgacagctg
    44941 ccttggccct cttgaacgac caagttaaaa agggtggagt gatggcctgt aaccagatcg
    45001 gtggtctatc tggtgccttt atccctgttt ctgaggatga aggaatgatt gctgcagtgc
    45061 aaaatggctc tcttaattta gaaaaactag aagctatgac ggctatctgt tctgttggat
    45121 tggatatgat tgccatccca gaagatacgt ctgctgaaac tattgcggct atgattgcgg
    45181 atgaagcagc aatcggtgtt atcaacatga aaacaacagc tgttcgtatc attcccaaag
    45241 gaagagaagg cgatatgatt gagtttggtg gtctattagg aactgcaccc gttatgaagg
    45301 ttaatggggc ttcgtctgtc gacttcatct ctcgcggtgg acaaatccca gcaccaattc
    45361 atagttttaa aaattaagaa aataggagaa attttaagtt ctatttaaga ttagatgtgt
    45421 atactataat cattaaataa agacctccta atattatttg aaacagataa cactgaatta
    45481 gtttgaattt gattttcatc taatatcttt atttaatgaa ctcctaaact ttttcataat
    45541 aatctccttc aaaagtcgcc tgtatgggtg gcttttattt tatcattcat gatataatag
    45601 aagcaaatgg aggacggaaa atggtaaaag tacgattgta tttggtacgt catggcaaga
    45661 ccatgtttaa cacgattggt cgcgcgcaag gttggagcga tactccctta actgctgaag
    45721 gtgaacgagg gattcaagag ttaggaatcg gtttgcgaga atctgatcta cagtttgagc
    45781 gcgcttattc gagtgattct ggtcgtacca ttcagaccat gggaattatc cttgaagaac
    45841 ttggcttgca ggggaaaatc ccttatcgca tggacaagcg tatcagagaa tggtgtttcg
    45901 gtagttttga tggagcctat gatggcgatc ttttcatggg cattattcct cgtatcttta
    45961 atgtggacca cgttcaccaa ttgtcttatg ctgaactggc tgagggcttg gtagaggtcg
    46021 atacagctgg ttgggctgaa ggctgggaaa aactcagtgg ccgaatcaag gaaggctttg
    46081 aaatgattgc aaaagaaatg gaagatcaag gtggaggtaa cgcccttgtt gtcagccatg
    46141 gaatgactat tggaaccatt gtttatctga ttaatggcat gcatccgcat ggtctagata
    46201 atggtagcgt gacgattctt gaatatgagg acggtcagtt tagggttgaa gttgtcggtg
    46261 accgtagtta ccgagagcta ggacgtgaga agatggaaga aggctctatt taatcagtct
    46321 agacttgctt gccatgagct agggatttga taagaatatc aagataagaa aaaacagccg
    46381 agggtactcc tttcggctgt ttttgatgtg gaaaactaaa gtgtaatgct attgctttta
    46441 gagattttca taaacaagag caaggaacct actgttagaa cagtcaggat agttgacaag
    46501 gctgcggcta caccgtaatt tcctctgaga acctctgtat aaatagctac agtcattgtt
    46561 cttgttttga cattgtagag gaggatagaa gtagagagtt ttgaaatcat tgtgacccaa
    46621 gataagatgg ctccagaaat gataccagat agcatcattg gagttgtaat cttagcaaag
    46681 gtattgagac gactacttcc taagctttca gcagcttctt caatacttgg tgctatttgt
    46741 tgtaagctag caacagatga gcgaatagta taaggtaatc ttctgacaga tagagacata
    46801 atcaagatga aagcagtccc tgtaatcata agaaatccac ttccaaatag accagtattg
    46861 aaggaagaaa tgaaggcaat ccctagaacg gttcctggta caatataagg taccatactg
    46921 aggctgtcaa ttaagtttgt aaacaaattc cgttttctaa cggctaggta ggagataaat
    46981 gtcgcaaata gaacaactag aactaaggca atcaaaggga tacgaatgat attgaaaata
    47041 gcagatccca tacgatggaa agctagcttg taactgtttg gagaataacc tttaacagat
    47101 accatacctg atgtttttag gaaagaggta taaattaagt agatttgagg taaaacagag
    47161 ataaagataa ttccgtagac tgttgcataa atggcagcta tttttccttt tgtagttttt
    47221 ttaggctcaa ttggatggag cagattcatg ctgaaactgt agcggtttgc aatgtgtttt
    47281 tggataagga aaattgccaa ggcaatgata atcgccataa ttgcaaaagc agaatttcct
    47341 ccaacctcgc taataaattg ggtataaatc aggacaggga aagtccgata tccttcgcca
    47401 atcaacatag gcgttccaaa gtctgagaat gctctcataa atacaagcaa ggagctgcta
    47461 gtaaggttgg aactaggaga ggtaaaacaa ccgttacgat aggtttaaat ccgaaggacc
    47521 ccatgctttc agctgcttca agtagagaat tgtcaatact gttcattgtt ccagcaacat
    47581 atagaaatac cagtgggaat agttgcagtg taaagacaag tacaattcct ttgaatccat
    47641 aaatatcgat agctggaaga tgaagggcat ttgtcaaaaa tttagtgatg accccatttc
    47701 gtcctagcaa gagaacccag gagtaggctc ctacgaaagg agctgacatg gaagcaatga
    47761 taatcaattt ttgtagaaat ttctccccct tgaagtcata catagagaag agataagcta
    47821 atagggttcc tacaactaag gaagtgacag tagcggtaat ggaaaccttg aaactgttga
    47881 ctagtgtctc agagtagtag actttactaa agaaagtgac aaaattagct agtgagaatt
    47941 gtccttcatg tataagtgct tacttgagca cggtaacgat aggataaacg agaaagacaa
    48001 gataggtaag aaagatgaag aaagaggagg ctgtccaaat atttagtttt ttacgttcca
    48061 tggttgactc cttttatcag gttttgggaa ccatctgcag aaaagatgtt taatttttgc
    48121 gtattgattc gtagacgaat acgattgcct ttttgtagat cttcttcaaa agttgattct
    48181 tcactaactt gaatttttga ggcaaaacct gtctcaatga aatactccgt atttagtcca
    48241 agatagacgc tatcgctaat agttccttca atatctctag attcatcttt gataaactct
    48301 tcgggacgaa tgcttacatg aatagcttgc tcctcaacct gatcaagagc tagcattcga
    48361 agggcatagc catctgaaaa gacgatataa gcgccgtcgc tccgtttttc aagattggca
    48421 gggataatat ttgtgcgtcc gataaaggtt gccacaaact cattagctgg tttatgatag
    48481 agttcttttg gtcggccgat ttgttggatc accccatctt tcataacagc aatttggtct
    48541 gaaatagcca tggcttcttc ttggtcgtgg gttacataaa cagttgtaat tcccacttcg
    48601 tgttggattt ctcggatggc ttgacgcata tccaagcgaa gtttggcctc cagattacta
    48661 agtggctcgt ccatgaggag aacacttaga ttaaccgcta aggcgcatgc caaggtgaca
    48721 cgttgttgtt gtccaccact gagtttatcg ggctttcgat ccgcatattg agcaatttgc
    48781 atgagttcaa gatacttgtt ggtctgttga atcaattctt cttttggaac cttcttttgc
    48841 ataagaccaa aagcaacgtt gtctcggaca gtcaaatgtg ggaaaatagc gtagttttgg
    48901 aaaaccatcc cgatattgcg tttgctgggt tccatattat tgatttttgt atcatcgaag
    48961 taaaattctc catctttgat actgttgaaa cctgcaatca tacgaagaag ggtcgttttc
    49021 ccacatcctg aagctccaag aagggtaaag agacttcctt ttggaattgt aatgttcaaa
    49081 ttctcaataa cagagacatc gtggtagatt tttttggcgt taataatttc gatctcactc
    49141 atagtgaacc tcttttactg tttagattgg atatctgtaa agacttcgtt gtatttctta
    49201 acgatatctg atttattctt gatgacataa tcataatctt cagtgagtgt tttgattttg
    49261 tcaattggtt tcatgttttc gcttgtttta gcatttttac gaacaggacg gttagtagtg
    49321 gttgtaccaa gtgtatcttg tacttcttga gagataataa aatcgataaa tttcttggca
    49381 ttttccatat ttttagattt tttaacgata gcagcactag caggtaggaa gacggttcct
    49441 tcttttggat agactacctt aatgttagct ccgtcattta agagtttaac tgctggatct
    49501 tcataagaga gaccaacagt catttctcca tcagcgactg ctttatagac accagatgaa
    49561 cttaaaccga ttttatagta tattgaaact agaataatac acatctactt ctaaaacatt
    49621 gttagaaatc gatttgactg tcctgattaa tttgtcctgt tcttatttca ttttactata
    49681 ccatcaataa gtgtgaaaag atcttttaca taagaccaag ccttatcatc tttgtaacca
    49741 ccttgagctt gtagcatatt tgttaattga gcaaaggcgc tagaagagtt tgctgggtca
    49801 gcagttgcga tttttccttt tagttcaggt ttgagaaggt cgctatatcc ttcgatgttc
    49861 atgcctttag ttaaatcagg gtggacgatt aaaacactac catctagtgt ataaggagta
    49921 gagaagccag ttgtgttttg atattctttg ataacattat cattttcttt tgaaatatag
    49981 ttttcaaaga gttctccgtg ggtagcatat tgtgtataag aaccaccaaa gataacatca
    50041 gctacaggaa cttctttttc tgactctagt tttttgaaaa gttctccagt accagcttga
    50101 atcagttcta ttttgatacc atatttttct tcaaaggcag gaatagttgc tccaattaag
    50161 ccttctgagt ttggtcaata aacgactagc gaaccaccgt ctcctttatc agatgaactg
    50221 tcatcggcag attcattaga agaacaagca gcataataca tccatttctt tttcatgatg
    50281 gatacctccg ttgtgttatt taagtttatt ttaaaacaat gtaagcgttt ttaaaaccta
    50341 caattctatt ctatagtgta ttgaatctat aacagtacac tttgactgct aaaatatttc
    50401 tataaattaa tttgactttc ctgatagaga tgttcacatc ttatttcaat tcactatatt
    50461 agagtaaaat tctctacaaa aagaagaata gcctatttta ctattcttcc gagtaatttc
    50521 aattcctttg gggaaatatg gagatgcttt ttaaatactt tggcaaaata tttatagtct
    50581 gaaaatccaa cttggtcaga gatagcgctt aaaggggagt tgggagattc agccatctta
    50641 tctatagctt gtttcaggcg atattgtaag atatattcat ttagagtcat ggggaagtct
    50701 tctttgataa ccttatatag atgcttgtta tctgcaaata gctagagcgt ttcaaggaaa
    50761 tgaactagaa ataacaatta atatggtggt tcagaaatac cttttgataa tcaataagat
    50821 cttcaggatg aagtgcgttg actccatcca tttcatattt ccgatttaat aaacgatatt
    50881 tgttttcacc aaattgatga aaagggagta gttgaacttg gtcgatattt aatgagttaa
    50941 atagagtagc gaatttttct gcatcctcta aactattgtt aaaattagga ataactggga
    51001 ttcttaaaac gatagtttta ttttgagaaa aagcataatg aatgttttta ataatcattt
    51061 gattaaaaac cccagtcact tttttatgtt ttatagaatt
//
LOCUS       DAVGJJ010000005        49571 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000005 DAVGJJ010000000
VERSION     DAVGJJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.005"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..908)
                     /locus_tag="VVE35_000256"
     CDS             complement(<1..908)
                     /locus_tag="VVE35_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEU3060514.1"
                     /translation="METKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSD
                     PSSLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKD
                     AKWFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTV
                     GVKALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGP
                     FLMKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARL
                     FPNSSSYEGIKEKYKNNIIYSMQNSTS"
     gene            complement(1105..2727)
                     /locus_tag="VVE35_000257"
     CDS             complement(1105..2727)
                     /locus_tag="VVE35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU3060515.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVVLVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            2833..4278
                     /locus_tag="VVE35_000258"
     CDS             2833..4278
                     /locus_tag="VVE35_000258"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU3060516.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLTGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(4324..4516)
                     /locus_tag="VVE35_000259"
                     /pseudo
     CDS             complement(4324..4516)
                     /locus_tag="VVE35_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            complement(4617..5225)
                     /locus_tag="VVE35_000260"
     CDS             complement(4617..5225)
                     /locus_tag="VVE35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU3060517.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(5250..5762)
                     /locus_tag="VVE35_000261"
     CDS             complement(5250..5762)
                     /locus_tag="VVE35_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU3060518.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(5848..6783)
                     /locus_tag="VVE35_000262"
     CDS             complement(5848..6783)
                     /locus_tag="VVE35_000262"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU3060519.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(6854..7153)
                     /locus_tag="VVE35_000263"
     CDS             complement(6854..7153)
                     /locus_tag="VVE35_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU3060520.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(7143..7892)
                     /locus_tag="VVE35_000264"
     CDS             complement(7143..7892)
                     /locus_tag="VVE35_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU3060521.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(7946..8305)
                     /locus_tag="VVE35_000265"
     CDS             complement(7946..8305)
                     /locus_tag="VVE35_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU3060522.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            complement(8307..9707)
                     /locus_tag="VVE35_000266"
     CDS             complement(8307..9707)
                     /locus_tag="VVE35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU3060523.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(10002..10241)
                     /locus_tag="VVE35_000267"
     CDS             complement(10002..10241)
                     /locus_tag="VVE35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU3060524.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(10273..10743)
                     /gene="ssbA"
                     /locus_tag="VVE35_000268"
     CDS             complement(10273..10743)
                     /gene="ssbA"
                     /locus_tag="VVE35_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU3060525.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(10755..11045)
                     /locus_tag="VVE35_000269"
     CDS             complement(10755..11045)
                     /locus_tag="VVE35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU3060526.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(11198..12544)
                     /gene="asnS"
                     /locus_tag="VVE35_000270"
     CDS             complement(11198..12544)
                     /gene="asnS"
                     /locus_tag="VVE35_000270"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU3060527.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(12560..12913)
                     /locus_tag="VVE35_000271"
     CDS             complement(12560..12913)
                     /locus_tag="VVE35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3060528.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLSPNSPHP
                     GGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYYAAFM
                     IDLDGNNIEVVCHKE"
     gene            complement(12906..14093)
                     /locus_tag="VVE35_000272"
     CDS             complement(12906..14093)
                     /locus_tag="VVE35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3060529.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(14090..14578)
                     /locus_tag="VVE35_000273"
     CDS             complement(14090..14578)
                     /locus_tag="VVE35_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU3060530.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            14899..15447
                     /locus_tag="VVE35_000274"
     CDS             14899..15447
                     /locus_tag="VVE35_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3060531.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(15724..16452)
                     /gene="yaaA"
                     /locus_tag="VVE35_000275"
     CDS             complement(15724..16452)
                     /gene="yaaA"
                     /locus_tag="VVE35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU3060532.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTLKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(16531..16695)
                     /locus_tag="VVE35_000276"
     CDS             complement(16531..16695)
                     /locus_tag="VVE35_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU3060533.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(16762..18357)
                     /locus_tag="VVE35_000277"
     CDS             complement(16762..18357)
                     /locus_tag="VVE35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060534.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            complement(18369..18779)
                     /locus_tag="VVE35_000278"
     CDS             complement(18369..18779)
                     /locus_tag="VVE35_000278"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU3060535.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEEEEGCLSLVGVRSTKRYETTRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(18895..19686)
                     /gene="yghU"
                     /locus_tag="VVE35_000279"
     CDS             complement(18895..19686)
                     /gene="yghU"
                     /locus_tag="VVE35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU3060536.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(19699..22395)
                     /locus_tag="VVE35_000280"
     CDS             complement(19699..22395)
                     /locus_tag="VVE35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU3060537.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMTGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            22699..23883
                     /gene="mntE"
                     /locus_tag="VVE35_000281"
     CDS             22699..23883
                     /gene="mntE"
                     /locus_tag="VVE35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU3060538.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(24026..25897)
                     /locus_tag="VVE35_000282"
     CDS             complement(24026..25897)
                     /locus_tag="VVE35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3060539.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25894..27078)
                     /locus_tag="VVE35_000283"
     CDS             complement(25894..27078)
                     /locus_tag="VVE35_000283"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU3060540.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(27090..27857)
                     /locus_tag="VVE35_000284"
     CDS             complement(27090..27857)
                     /locus_tag="VVE35_000284"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU3060541.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGM
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(28134..28982)
                     /locus_tag="VVE35_000285"
     CDS             complement(28134..28982)
                     /locus_tag="VVE35_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU3060542.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLGEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(28984..29358)
                     /locus_tag="VVE35_000286"
     CDS             complement(28984..29358)
                     /locus_tag="VVE35_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU3060543.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(29432..30784)
                     /locus_tag="VVE35_000287"
     CDS             complement(29432..30784)
                     /locus_tag="VVE35_000287"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU3060544.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(30808..31587)
                     /locus_tag="VVE35_000288"
     CDS             complement(30808..31587)
                     /locus_tag="VVE35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU3060545.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(31574..32431)
                     /locus_tag="VVE35_000289"
     CDS             complement(31574..32431)
                     /locus_tag="VVE35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU3060546.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            32567..33535
                     /locus_tag="VVE35_000290"
     CDS             32567..33535
                     /locus_tag="VVE35_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU3060547.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(33569..34480)
                     /gene="whiA"
                     /locus_tag="VVE35_000291"
     CDS             complement(33569..34480)
                     /gene="whiA"
                     /locus_tag="VVE35_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU3060548.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(34477..35454)
                     /locus_tag="VVE35_000292"
     CDS             complement(34477..35454)
                     /locus_tag="VVE35_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU3060549.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGIEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(35451..36341)
                     /gene="rapZ"
                     /locus_tag="VVE35_000293"
     CDS             complement(35451..36341)
                     /gene="rapZ"
                     /locus_tag="VVE35_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU3060550.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLLELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(36393..36773)
                     /locus_tag="VVE35_000294"
     CDS             complement(36393..36773)
                     /locus_tag="VVE35_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU3060551.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(36784..37371)
                     /locus_tag="VVE35_000295"
     CDS             complement(36784..37371)
                     /locus_tag="VVE35_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU3060552.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(37380..38612)
                     /gene="clpX"
                     /locus_tag="VVE35_000296"
     CDS             complement(37380..38612)
                     /gene="clpX"
                     /locus_tag="VVE35_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU3060553.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(38814..39320)
                     /locus_tag="VVE35_000297"
     CDS             complement(38814..39320)
                     /locus_tag="VVE35_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU3060554.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQVQEKNLYIIGG
                     KQIFQAFGPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(39450..39968)
                     /locus_tag="VVE35_000298"
     CDS             complement(39450..39968)
                     /locus_tag="VVE35_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU3060555.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(40465..41970)
                     /gene="lytC"
                     /locus_tag="VVE35_000299"
     CDS             complement(40465..41970)
                     /gene="lytC"
                     /locus_tag="VVE35_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU3060556.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTLQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(42008..42766)
                     /locus_tag="VVE35_000300"
     CDS             complement(42008..42766)
                     /locus_tag="VVE35_000300"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU3060557.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(42864..43541)
                     /locus_tag="VVE35_000301"
     CDS             complement(42864..43541)
                     /locus_tag="VVE35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU3060558.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            complement(43550..44494)
                     /locus_tag="VVE35_000302"
     CDS             complement(43550..44494)
                     /locus_tag="VVE35_000302"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU3060559.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            complement(44676..45188)
                     /locus_tag="VVE35_000303"
     CDS             complement(44676..45188)
                     /locus_tag="VVE35_000303"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU3060560.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(45275..46033)
                     /locus_tag="VVE35_000304"
     CDS             complement(45275..46033)
                     /locus_tag="VVE35_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU3060561.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            46238..46384
                     /locus_tag="VVE35_000305"
     CDS             46238..46384
                     /locus_tag="VVE35_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060562.1"
                     /translation="MEIKEQTRKLAVSCSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(46531..47661)
                     /gene="ugpC"
                     /locus_tag="VVE35_000306"
     CDS             complement(46531..47661)
                     /gene="ugpC"
                     /locus_tag="VVE35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU3060563.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(47944..48029)
                     /locus_tag="VVE35_000307"
     tRNA            complement(47944..48029)
                     /locus_tag="VVE35_000307"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47993..47995),aa:Leu,seq:aag)
     gene            complement(48111..48686)
                     /locus_tag="VVE35_000308"
     CDS             complement(48111..48686)
                     /locus_tag="VVE35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU3060564.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(48686..49474)
                     /gene="codY"
                     /locus_tag="VVE35_000309"
     CDS             complement(48686..49474)
                     /gene="codY"
                     /locus_tag="VVE35_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU3060565.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEALVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT        14390 a        11338 c         9257 g        14586 t
ORIGIN      
        1 gaagttgaat tttgcatact atagatgata ttgtttttgt atttttcttt aatcccttca
       61 tagctggagc tgttaggaaa aagacgagcc gtagtataag caccagctgt aaaattacgt
      121 tccagtgatt cttggtcgct accatcatag taggtcaatt tcacatcgtc tacaaagaca
      181 ttcttagcat cccagtaatt agggtttttc ttatattcaa tagcagattt tgagacaagt
      241 gctttcatca agaaaggtcc attgtacaaa atactagatg gatccgcctt cccaaaatca
      301 tccccttttg atttcaggaa atctgcatta acaggaaaaa gtatcgttgc aagtgttttt
      361 gaattccagt aaagttctgg tttaaccaaa gtatattgaa ccgtttggtc atcaagtgcc
      421 ttgacaccga cagttgaaaa gtcgcttgtt ttaccagtga tatagtcatc caaaccagca
      481 acagagtcct gcactagata caaagcttct gattttttat cagctgcata ttgcaaacct
      541 gtcacaaaat cctgggcagt tacaggcgca tattcttctc cctcagaagt aaaccacttg
      601 gcatccttac gaagtttgta ggtataggtc aaaccgtcct gagaaacagt ccaatcctct
      661 gctaatgatg gaataatatt cccatattgg tcattttcta ataacccgtc taccaaattt
      721 gcaacaatat cggatgttgc tgcgcggttt tctgctagat agttcaaact agatggatca
      781 cttgaataaa catagttata ggtttttgat gcagtgctag aatttccaca cgcgctcaat
      841 aaaactcctg tacccagaac aagacctgcc aaggttagat atttgctctt agactttttc
      901 gtttccagaa cctcctgctt taaatatttg ttttattata caactcttgg tttgtttagt
      961 caattgtttt ttccaaaaaa tcaaaaaaaa ctcattttct atttttgaaa gataatttta
     1021 gctattttga cgctaaaaaa ggaataagtt tcaagttctc ctaaaagatt gttcaactta
     1081 ttccttttat ttataaatgc taccttacga aagcttaaat tttgctcgca ggcgatctgc
     1141 ttgggctttt cctattacct tatctaataa ataggtacgc agactcataa ccatataaag
     1201 tccacccccc atggcaccga caagaactac ataaaagaag ctccacaaac gtccacttgg
     1261 ttggaagaaa aatcctaaca gccactggat ggttcctatt aacagaaaca tgactagggt
     1321 cagcaaactg attaaaatgg ttcgcttcaa aatcaccttg cgcttgacac cagttacttt
     1381 acaaatatcc cgatacatca agacgttagg aatgatgaga gcaatggttg ttgaaatcaa
     1441 aggaccataa ctgtggaaga gggcgatggt aggtagttgc aagactagct tggcaataga
     1501 accatagata aaatagagaa cggccttgcg gttgcggaac atggcctgaa gcattggaga
     1561 caagaccatg tacaaaccta aaataataga ctgcaaaact gcaaagacaa ataagcccag
     1621 agccaaacta tctggcttac catagaagac tgtatataga ggttctccta ccataaccac
     1681 tccaaccgtt gctggtagca agaacataaa gagtagggtg agactgtcct gaaccagacg
     1741 agaagctgct ttcaagtccc ccttgacata gttttccgtc aaaagtggca aaccaacact
     1801 cccaatcgaa acccctacag aaatcaaaat catcgtgatt ttattaggat tggctgagaa
     1861 ataagaaaac atgacaacca agtcctcatt gctgtagttg gtaaaccagc tcatactatt
     1921 gataaaggtc agctgatcca aaatctggaa gagctggatg gcagaccctg tcaggataaa
     1981 aggaatggct tccttaatgg tatcaaccaa gagacgctta ctgttaatct tatctcctgt
     2041 ttcaaagact cttttgagtg aaccttcttg ggcaaggaaa taaatcaaga ctgcaaaact
     2101 ggctaccata ccgacaaagg cagcaaaggt tgattgggta acggctgcta gataatctcc
     2161 tgaaccgagc ttcataatga taaaggttgc taggagcatc cagataacac gaatgacctg
     2221 ctcagcaatt tggctcatgg cataaggttt gaggttattc atcccttgga aaaatcctcg
     2281 gataacactc atagacggga aaatcaagac tccccaagcc aagctttgca tgattgggat
     2341 caagtctttg cccacgccag acaagtctgc tagccaagga gcaaagacat acaagactaa
     2401 agcaaaaacc aggcctagtc ctgtcataaa gcctaagaag ctccgaatca gggcaaagct
     2461 atgctcttct tctcgcatgg tattatactt ggcaacttgc ttggccaccg caactggaat
     2521 ccccgctgtt gaaaccaaca agaaccaagc atagatattg taacccatgg taaagagacc
     2581 atttgcctta gctgcataag cccccatcca gatgtaccaa gggataatgt aaacagcccc
     2641 gagtaggcga ctgataaagt tactagccgt tagccaagca gtcccccgta acatctgggc
     2701 ctgctggtga ttgttttcgt gcgacataga ttcctcattc aattttgata actaggaaaa
     2761 actccttatc ttccattata aacttttcct tgttacttgt aaatttacga ctttcggttt
     2821 acaatagaaa gtatgattaa gattgaaacc gtattagata ttttaaagaa agatggcctt
     2881 tttcgcgaaa ttattgacca aggtcattac cactacaact acagcaaagt tgtttttgat
     2941 agcatcagct acgacagccg aaaagtaaca gaagacactc ttttttttgc aaaaggcgct
     3001 gcctttaaaa aagaatacct tctttctgct ataacacaag gtttagcttg gtatgtagct
     3061 gaaaaggact acgaagtcga tatccctgtc atcattgtga acgatataaa gaaagccatg
     3121 agtttgattg ccatggagtt ctatggtaat ccacaagaga aactcaaact ccttgccttt
     3181 actggtacta agggtaagac aacagcaacc tatttcgcct ataacatctt atctcaaggg
     3241 catagacctg ctatgttgtc gaccatgaac acaactcttg atggcgagac tttctttaag
     3301 tcagcgttga caacccctga gagtattgac ctctttgaca tgatgaatca ggctgtgcaa
     3361 aatgaccgta cccacctcat catggaagtc tccagtcaat cctatctggt caaacgtgtc
     3421 tatggtctaa cctttgatgt gggagttttc ctcaatatca gcccagacca tatcggcccg
     3481 attgaacacc ctagctttga agactatttc taccacaagc gtctcttgat ggaaaatagc
     3541 cgagcagtca tcattaacag tgacatggac cacttctcag tcttgaaaga acaggttgaa
     3601 gatcaagacc atgatttcta tggtagccaa tttgataacc aaatcgagaa ttccaaagcc
     3661 tttagctttt cagctacggg taaactcact ggagattatg atatccaact cattggcaac
     3721 ttcaaccaag aaaatgcagt tgctgctgga cttgcttgtc tccgtctcgg agcaagtctt
     3781 gaggacatca aaaaaggcat cgctgcaacc cgcgttcctg gtcgtatgga agtcctcact
     3841 cagaaaaatg gagccaaggt cttcatcgac tatgcccaca atggggatag tctgaaaaaa
     3901 ctcatcaatg tggttgaaac tcatcaaacc ggaaagattg ctctggttct gggatcaaca
     3961 ggaaacaagg gagaaagtcg tcgtaaggac tttggcctcc tcctcaatca acaccctgag
     4021 attcaagtct ttctgactgc tgatgaccct aactatgaag acccaatggc cattgcagat
     4081 gaaattagta gctacatcaa tcatcctgtt gagaagattg cggatcgcca agaagccatc
     4141 aaggcggcaa tggctatcac aaatcacgaa ttagatgcag ttattattgc gggtaaggga
     4201 gccgattgtt accaaatcat ccagggcaag aaagaatcct acccaggaga tacagccgtc
     4261 gcagaaaatt atttataaga atagaaaaaa tcaagggaaa atgaaaccct tgattttttc
     4321 ctattattta gtaaaaacgt ttttcaaacc ttcaacggca ccttctacag caccttttgc
     4381 gtcttctaca acgtcttttg ccttagcaac tgttttttac agctccttca agttctgtct
     4441 tgctgtctcc agtaacttta ccaaaacctt ctttgacagc gcctgttgct tgttccaatt
     4501 tgttttcaag tgacatatga ttgtctcctt tagtttattc cgatatgtgt taccggttac
     4561 atatagttta taccgaatac taaggaaagt caaacaatgt actcagaaac aagaaatcac
     4621 gctaagtaga aagttaatct ctccttatca aaatcgatag ccaactcata ctttccatct
     4681 tcattttgca caatatagcc taagacaacc aaactgtcca caaagatatc acggcatttc
     4741 tgcataagct ggtctttttt gagaaatttc aacaaaaaag ccgtcatata cttgagggca
     4801 tactcaggat taacatctcc taaaatgtca tagagctcct gctgtttttc tgtcaaagga
     4861 tactgctgtt tgaccttgta aaaataattg gacagggtca ttttaatgcg cgcaaagtcc
     4921 gtcttttcaa ctaaaatagc tgcattgatt tgattgcgca attccgtctc aaaactctgc
     4981 tccaacaagg cttgatagac cggactagct tctctgacaa aaatctcttg atccagttca
     5041 agactatcaa gtgattcaag cgtaggaaaa ttgaggtaat agcgcttatt ttcacgtaga
     5101 atcaagcctg cctttatata ctcttccatg agtttatcaa ctgcaacatc tggaaattga
     5161 gccttaattt cccgtagaat cacatcctca tactggtcca gatagcggat caattctcca
     5221 aaaatggctg tctcgtcaaa cgagatggat taaaaatctg aatcatgaag attcctttct
     5281 ttacattgta acagaggcga tgctgccaac tgactaggat tggtaccagc ttggttcaga
     5341 ggaacaggca gacctagttc tatgtaatac tccctccaaa aatcactaat ctcctgctcc
     5401 ccatctccaa atttcatagg aatagtttca aaaataggta ggatttctgc tatatactgg
     5461 gagcagtaga aaccagctgc atctggatag aaagaagcat tgtagggtgc tccaagatgt
     5521 ttgcaagctc tttccttcac cgactggata tccatttctg ggtaaacata gaggtcgtat
     5581 aaatgattgg actcaaagaa gtctgccggt tcttgacaga caacaccagc ctgtccacta
     5641 gcatgataaa tcatcccatc caaataaatg gcaacatggc tatagttacc tgtggaagtc
     5701 tggatggcct gtcccatgtc tgacccatct ctcacaaaaa tcaaatcgcc attttctaac
     5761 atacttcact cctagaaaaa aggagccgaa actcctttcg atataaggca aactagactt
     5821 tccgattttg agctgacacc caaaatctta cgcattaaag ctttcagtca attgaggtac
     5881 cacttgtttc ttacgtgaaa cggcaccagc aaggaaggca tgattgtttt caagtttgaa
     5941 gttgaaagcc gcttcgacct tgtccatatt ggcaccaaga gccaagattt ctgagtttga
     6001 gttgacgata tctgtaatca tcaagacaaa gtcagagtag ccgtttgatt cgttggcagc
     6061 ttgcattgca gcttcaattt ctgcttggcg ttccaaaact tcagcgatgt caactgtgtt
     6121 cacttgggca acacggacat tatttccgtt gagttcaaaa gtcttagcat cgatgtcaat
     6181 caattcttca gcagatttgc tagccaagtt ggtaccagct ttcaacattg ccaaaccata
     6241 ttcttccaag ttcacaccag ccaattcagc caattcagga gcaatgattt tatctgttgg
     6301 gtgtgttgtt ggtgatttca aaagaagggt atctgaaatc aaacctgaaa gcatcaaacc
     6361 tgcaatctct ttaggcacag ctacaccatg ttctttgaac atacggtaaa cgattgaaga
     6421 cgctgatcca actggctcca aacgcatgta aagtgggctt gcagtttcaa agttagccac
     6481 acggtggtgg tctacaacac cgtaaacttc tacttcagcg atatctgata cagattgttg
     6541 gaattcattg tggtcagtca agataacttg ctctgccccc tctgctttgg cagaagtgat
     6601 aacacgtggt gcttccacac caaaatagtt caagacaaag gctgtttctt catttggagt
     6661 tccaagggca acagcttccg tatccaaacc gtaagcttct tttgcaaggt aggcaaaagc
     6721 tacagatgat ccgatggcat ctgagtctgg attttggtga ccaaatacta gaatcttgga
     6781 catgataata cctcattttg tttattttct ttattgtagc atttttttcg ctttttgaca
     6841 aaggtaagag gagtcaaagg actccccacg attcttcaaa ataactgagt aaatttctat
     6901 cttgattttc agataaaaat tcttccttct gtggcctctt cttacgcttg agaagagctt
     6961 cagccgacat ggcttcttcc ttactggcaa aaccttgagc atagataagt ttgactggca
     7021 agcgtgctcg tgtatatttg gctcccttcc cactattgtg gatagcgagg cgtcttctca
     7081 catcagtcgt atagcctata tagtaggatc catcacgaca ctccagaacg tacatataag
     7141 ccttatgatc cataataaat ctcttcgatt tcgggcgtat aagagccatc atcattgtgg
     7201 acaatcaaag gaggtaagac cttaaagcca cttgttgagc catccttgat cgcctcaatc
     7261 aaaagcatat tggcttcctt ttctcttttt ggataaacaa actgcaggcg cttaggggct
     7321 agattatgtc gttttaacgt atccaaaata tccagaagtc gatcaggacg atgaaccatg
     7381 gccaaacgcc cattagactt gagaatactc tgggcactat gacagatttc ttccaaatta
     7441 gtcgtgattt cgtgtcgagc caagagataa tgttcactct cgttcagatt agaatgagga
     7501 tccaccttga aatagggtgg attacacaaa atcatatcca ccttactccc ctgaatgtga
     7561 gcaggcatat ttttcaaatc atcgcagatg acctgcattt gctcctctaa tccattcaaa
     7621 cggacagagc gttcagccat atccgccaaa cgctcctgaa tctcaacaga caatatctgt
     7681 gcttgagtac gagtgctagc aaaaagcccc actgctccat taccagcaca gaaatctaca
     7741 atcaacccct tcttaggaaa acgtggaaat cgtgataaga gaacactatc caccgaatag
     7801 ctaaaaacct ctctattttg aatgattttg atatctgtcg aaaagagctg gttaatgcgc
     7861 tctcctgatt ttaataattg ttcttcttcc atggtcctat tatagcaaat ttatattaac
     7921 attacaaaaa atataaaact ctaaactact tcttcttttt taaatagtgc agggcttctc
     7981 cagtccagat tggtagcatt cgtcgaaagg gagcaaaacc gtagttaaag cggtcgcttg
     8041 aaaagcgtct ccgtctagga aactggtact tttcttcctc caaagtgcgg atagaaagac
     8101 tggctttccc cgtaaattca tctaaatcca ctacctgaac ttgaacctct tcatcgactt
     8161 tcaaggtttc atgaatattt tcaataaatc ctgtccgaat ttctgaaatg tgaatcagcc
     8221 ccgtatcacc cgtctctaac tcaacaaagg caccgtaggg ctgaatccct gtaatacgcc
     8281 cctttagctt atcaccgatt ttcatcttag tcctcgattt caatagtttc aattacaaca
     8341 tcttcaactg gcttgtccat agctcctgtc tcaacagcag caatggcatc caagacagcg
     8401 taagatgctt catcagctaa ctgaccaaaa accgtgtgac ggcggtctag gtgaggtgtc
     8461 ccaccttgat tggcatagat ttctgcaatc ggttctggcc aaccaccacg agtaatttct
     8521 ttcttagaat aaggtaggtg ttggttttgc acgataaaga actggctgcc gttggtattt
     8581 ggaccagcat ttgccatgga aagagcacca cggatattgt aaagctcttc tgagaattca
     8641 tcctcaaaag attcgccgta gattgactcg ccacccatac cagttccagt tgggtctcca
     8701 ccttggatca taaagtcctt gataatacgg tggaaaatga caccatcata gtagccatct
     8761 tttgaaagag atacaaagtt agccactgtt ttaggagcat gttcagggaa aagcttgata
     8821 cgtaagtctc cgtgattggt cttaatagtc gcaagaggac cttctactgt ttcaatgtct
     8881 acttgtggaa aatgcaattc tttttctacc ataccaaata cttctaaggc atcaaaaatg
     8941 ccatcttctt ctaatgtttt tgtaatataa tctgcttttt ctttgatttt atcatgagaa
     9001 attcccatgg caacgctgat tccagcataa tcaaagagtt ccaagtcgtt gagaccatct
     9061 ccaaaaacca tgaccttctc tggtttcaag ccaaggtgtt ccacaacctt ttccaccccc
     9121 gtcgctttgg agcctgaaat cggcacaata tcagaggaat gttgatgcca acgaaccatg
     9181 cgaagtttgt ctgagagact gtcaggcaag tgcaagtcat ctcccttatc ttcaaaagtc
     9241 cacatctgat agatatcttc tttttcatgg aaatcgggat ctacatctaa gtcgggataa
     9301 attggattga tagcttcact catcatatcg gtgcgagtcg acaacttggc atcatgactc
     9361 ccaaccaagc catactcaat tccttcttgc ttagcccaag agatatactc ctcaacatct
     9421 gacttttcaa tctgatgctg ataaatgacc tgaccttttt tatcttcgat ataagcccca
     9481 ttcaaagtta caaaaaagtc aggcttgaga tcacgaatct ctggaacaac accaaaaatg
     9541 ccacgtccag aggcgattcc tgttaaaatt cctttttcac gcaactgttt aaaaacagtg
     9601 ggaattgtag ttggaataaa ccctgtcttt gaattccgca atgtatcatc aatatcaaaa
     9661 aagacaatct tgatcttctt tgccttgtat cttaatttcg cgtccatctc actacctctt
     9721 tcaatctaac tctttccatt atatcataaa gtaggcaaat cccctatttt caaaaagttt
     9781 atcattttta ttttaatttc ttggatgaga aaagagacat atttatgaaa aagctccatc
     9841 gtgcttttaa tgtgttctct tgttttcaaa ctcgtaaaaa gggagccact gagcctaact
     9901 cgctctctca tttcaaagct tgtgaaaaaa gacccgttgg ggtcttaatt cgctttcttg
     9961 ttttcaagct catgaaaaag agacccaact gggtcttttc tttaatcttc gtttacgaaa
    10021 ggcatcaaag ccattacgcg agcgcgtttg atagctgttg ttactttacg ttggttttta
    10081 gctgaagttc ctgttacacg acgaggaagg attttcccac gttctgaaac gaaacggcta
    10141 agaagctcag tatctttgta atcaacatat tcaattttgt ttgctgcgat gtaatcaact
    10201 tttttacggc gtttgaatcc gccacgacgt tgttgagcca tgttttttct cctttataag
    10261 tttagttgtc cattagaatg gtaaatcatc atctgaaata tccaatgggt ttgttgctcc
    10321 aaatggattt tcattacgtg aaaagtctgg tactgaattt gtaggtgctg aatagtttgc
    10381 agttggtgca gagtaagctc cacctgtgtg accctcacgc acactacggc tttccaacat
    10441 ttggaaattc tcagccacga cctctgtcac gtagacacgt tgtccttgct ggttatcata
    10501 actacgagtc tggatacgac ctgtcacccc gataagtgag ccttttttag cccagttagc
    10561 aagattttca gcctgttggc gccacataac gacattgata aaatcagcct cacgttcacc
    10621 attttgactc ttaaatgtac ggtttactgc aagagtaaaa gtcgcaactg ctacatttga
    10681 tggggtataa cgcaactcag cgtcacgtgt catacgccct acaagtacaa cattgttaat
    10741 catagtttac cttcttacgc gtcaattttg acgatcatgt gacgaagaat gtcagcgttg
    10801 atttttgaaa gacggtcaaa ctctttaaga gctgcatcgt catttgcttc aacgttaacg
    10861 atgtggtaaa gtccttcacg gaaatcttgg atttcgtatg caagacgacg tttttcccaa
    10921 gtttttgatt caacaacagt tgcaccgttg tcagtcaaaa tagagtcaaa acgtgctacc
    10981 aaagcgtttt tagcttcttc ttcaatgttt ggacgaatga tataaagaat ttcgtattta
    11041 gccattgata tgttcctcct tttggtctaa tgaccccaag actttgcaag gggtaagtga
    11101 ggttcgctca caataaacta ttatactaga aaaaattttt ttacgcaagt aaaaacacta
    11161 gaattcgaaa aaacgccaca tgggcgtttt cctgttctta tggtttgata cggtgcaaca
    11221 tacgtgggaa tggaatagct tcacggatat gttttgttcc tgctgcgaag gttaccatac
    11281 gttcgatacc gataccaaat cctccgtgtg gaactgtacc gtatttacga aggtcaaggt
    11341 agaattcata ttctgtacga tccatgccaa gttcatccat cttagcgaca agggcatcgt
    11401 aatcttcctc acgcatagac ccaccgataa tttctccata gccttctgga gcaagcaagt
    11461 ctgcacaaag cacgcgctct ggatttccag gaactggttt catgtagaag gccttgatgg
    11521 ctgctggata gttcatgaca aatgttggca caccaaagtg gtttgaaatc caagtttcgt
    11581 gtggtgaccc aaagtcatca ccatgctcaa gatgctcgta gtcagcatct tcatcatttt
    11641 catgctcttg caagaggtca atggcttgat cgtaagtgat acgtttgaat ggctctgcaa
    11701 tgtagcgttt caagagttct gtatcacgtt ccaaggtttc caaggcttga ggcgcgcggt
    11761 caagaacacc ttgtagaaga gctttcacat aagcttcttg caagtcaagc gactcatcat
    11821 gtgtcaagta tgagtactca gcatccatca tccagaactc agtcaagtga cggcgtgttt
    11881 ttgatttttc agcacggaaa actggaccaa agtcaaagac acgaccaaga gccatagccc
    11941 ctgcttctag gtaaagctga cctgattggc tcaagtaggc tggcgttccg aagtagtcag
    12001 tttcaaagag ttctgtagaa tcttctgccg catttcctga aagaattggg ctgtcaaact
    12061 tcataaaacc gttcttgtca aagaactcat aagttgcata gataatagcg ttacggattt
    12121 gcaacacagc tacttgctta cgagagcgta gccacaagtg acggttatcc atcaaaaagt
    12181 ctgttccgtg ttcttttggt gtgattgggt agtcttgaga ttcaccgatc acttcgatgt
    12241 ctgtgatgtc caactcatag ccaaatttag aacgttcgtc ctctttgaca atacctgtca
    12301 cataaacaga cgtttcttgg ctcaagcgtt tgataacatc aaacttctca agtcccactt
    12361 cttcaccaaa tttttcgaca aagtttggtt taaaagccac accttgaaag aaagctgttc
    12421 catcacgcaa ttgtaagaaa gcgatttttc cttttcctga tttgttggca acccaagcgc
    12481 caatcgtcac ttcctgacca acatagtctt ttacgtcaat aatcgttaca cgttttgtca
    12541 ttatttttcc ttttcttttt tattctttat ggcaaaccac ctctatattg ttcccatcca
    12601 ggtcaatcat aaaagcagca tagtaaatcg gatgctcact tcgataacca ggagccccat
    12661 tgtctcgccc acctgcctct aagccagcct cataacaagc ctgaacttct tccttatttt
    12721 ctgctaaaaa agcaaaatga acaggatctt gtgttccctg agtcagccaa aaatcaccac
    12781 caggatgagg gctgttcggg gatagagaac tagtcttaaa agccaattta tagtccaaag
    12841 gagcgagaaa actcctataa aatccttctg aaatttgtaa atcctttacc ttaatctcaa
    12901 aatgatcaat cattctcact acccataaat gctttcaagc gttcgactgc ttctttaagc
    12961 gtgtctaggt ctgtcgcata gctgaggcgg acattttctg gtgctccaaa tccagctcct
    13021 gttaccaagg ccacttcggc ttcttctaag ataacagttg taaagtctgt cacatccgtg
    13081 tagcctttca tctccatggc ctttttgaca tttgggaaga gatagaaggc cccttgcggt
    13141 ttgaccactt caaatcctgg cacttctgca aggaggggat agatggtatt aagacgttcc
    13201 tcaaaggcct gacgcatgct ttctacagta tcttgctcac ctgatagagc ctcaactgct
    13261 gcatattggg ctactgctga cggattcgaa gttgtttgac ctgcaatctt ggacatggca
    13321 gcgataatgt ctgcttctcc aacggcataa ccaatccgcc aaccagtcat ggcataggtt
    13381 ttagacacac cattgatgac cactgtttgc ttgcgaatcg cttccgatag gctagaaatc
    13441 ggtgtgaact catgaccatt ataaaccaag cggccataga tatcatctgc taggatgaga
    13501 atatcatttt ctacagccca gtttccaatt gccaagagtt cctcacgggt gtaaatcata
    13561 cctgtgggat tagatggcga attcagcacc aaaaccttgg tcttgtcagt gcgagctgct
    13621 tctaactgat ctacggtcac cttaaagtga ttgtcttcct tagtagaaac aaagacggga
    13681 acgccttctg ccatcttgac ctgatctcca tagctaaccc agtatggggt tgggatgatg
    13741 acttcatcac ctggattgac cacagccata aagaaggtat agagagaata tttggctccc
    13801 gcagcgactg tcacttgatt tgacgctaca gaatagccgt aaaagcgctc aaagtagcta
    13861 ttgaccaccg ccttaagctc tggcagacct gaggttactg tataaaaaga agcacgccca
    13921 tctcgaatcg atgcaatggc ggcatcttgg atatttttgg gagtagtgaa atctggctca
    13981 cccaaggtta gagacaaaat atctctgccc tcagccttca gtgctttggc acgggctcca
    14041 gcagccaaag tcacactttc ttccatttct aaaacacggt tggataattt cataggccct
    14101 ccttgttgac caatgctcct gtttcaaaat ctactagata aaaatcagat cctgacttga
    14161 cttcccagat tggcttatct tgataacgac caaagataat cttgtcaatt tcgccagctc
    14221 ccttttcctt agaaaccgtt tctgcttttt cttgtgaaac accctgattt agctgataaa
    14281 cgtaaatctt atgatcatct tttccaatca gaacagcaag tgcttcttgc tgtttattac
    14341 gaccaagaac gctgtaataa gattccaagc cattgtataa atcaacctga tcggcctgct
    14401 ctaatcctgc atactgctga gctaattttt ctccttcact tttagctgtt tgatagggtt
    14461 tcatgctaag agaaaccata tacagaaagg aagcactgat aaccacaaac aaaatcgtca
    14521 tccctagacc atactgccac agtagattat tttttgcttt gttttgtctt tttttcactc
    14581 gtctatttta ccatctatta agctttatta caagtgaata taagaatact cttcgaaaat
    14641 ctcttcaaac cacgtcagct ttatctgcaa cctcaaagct gtgctttgag caaccaattc
    14701 tatttctccc ttcaaacaaa accgattttg aaagtgaaac agttcttact ttttcagtca
    14761 caaatgacta gagtttccga aattttacaa ttttcaaata aactctgagc aaatttcttg
    14821 caagttcttt tgttttgttg taatatattt tataacaacg agagagttct cgaaatttta
    14881 agaaaaagga gacacatcat gtctaaaaaa gtattattta tcgtcggatc actacgtcaa
    14941 ggttctttca accaccaaat ggcgctcgaa gctgagaaag cacttgctgg taaagcggaa
    15001 gttagctacc ttgattattc tgcccttcct ctcttcagcc aagatttgga agttccaaca
    15061 catccagctg tagctgctgc tcgtgaagca gttctcgttg cggatgctat ctggattttc
    15121 tctccagtct acaacttctc tatccctggt acagtgaaaa acttgcttga ctggctatct
    15181 cgtgcccttg acttgtctga tacacgtggc gtttctgccc ttcaagacaa gtttgtcaca
    15241 gtatcatctg tagccaatgc agggcacgat caacttttcg ctatctacaa agacctcttg
    15301 ccatttatcc gtacacaagg cgttggtgat ttcactgctg cacgtgttaa tgactctgcc
    15361 tgggcagacg gaaaattggt tcttgaagaa acagtcctaa actcacttga aaaacaagct
    15421 caagacttgg tcgaagctat caagtaacta acactcaata aaaatcaaaa agcaaactag
    15481 gaagctagcc gcaagctact caaagcactg ctttgaggtt gtagatagaa ctgacgaagt
    15541 cagtaacata tatacggtaa ggcgacactg acgtggcttg aagagatttt cgaagagtat
    15601 aacataataa aagcgaacaa gactactttt ctgacactca gaagactagc ttgttcgctt
    15661 ttttctcatt tataaactaa aatagctgat gatacatatt gccaaataca aaatatctga
    15721 tttctatact tcctttacaa aaaccaatcc ctgtggttgt gacaaatctt ctcggtaaac
    15781 aaatccagca aagttcaagc gacgtgcttc ccccacagtt tgtacttgat tttctattaa
    15841 agctgttaga aaggccccgc gcgctttctt ggagatagtt gagtgaatct tcagctgacc
    15901 gcctctatcc tccatgaatt ttaaggtcac catctttgct ctgatttcct tagaaaatac
    15961 agtctcaaac tctgatgaca agagagagaa aatcacttct tccttcttca gagtttcatc
    16021 ataggctgcc ttccaatggc tcttcaaagt cttaccagcg acttttaatt tcatcaaaaa
    16081 atccaaacgg tgaggagcca tgggtgacaa gactggaaca acaccgtaca aagccgaggt
    16141 aatgaaaaca tgattttcaa gataatcttg ttccgcctcg gtcagcttat ctctcttaat
    16201 gttgcggtac ataagcccat caaaaagttt caaggctgga tagtgttgag cagtttgcct
    16261 tttcaaagct tggatatttt gaaattcttc cgccgctttc tcagctgata ccttgtagaa
    16321 actctccaat tgactggcag aatagagagc caaggcatca agcacggcct gactttctgg
    16381 ttttaaagga attgcctcga tacttggcaa gtctgtgttc atttcttttg ctgttgggat
    16441 taaaattttc atattgatag tgtagcatat tttttagcca caaccaagaa attcatttga
    16501 aaaacctcgg tttgaccgag gtttttctat ttatttgggc catcctaacc agacagccac
    16561 gagaacaagg gctgtgccag ctaaacttgt taagatagat aagaatttat cttttttctc
    16621 cttgaactga gaaaaaccta tcttcaaagc gagcagtccg agtagcattg aagcaaccat
    16681 caaataaaaa cccatttctt tgtcctccat taggcttaat ttaccttatt ataacatata
    16741 ctttctgaaa aaaacctatt tttaccgtag cttaaaggtc ttgagataat tgtcttttcc
    16801 tacttgacct tcgaaggttt taccattttc aaggtaagga aggtcatcgg atactgaagc
    16861 cttgaccttg tatatcttgc catcaacttt aaagaagtag acagtgtctc ccttgataac
    16921 agctgatttg aggtctgcta ctactccatt gatgctttct gtcgttgcat tgtcaatttc
    16981 aaggtcgttt ttattggcat acttgctgag catctcttcc actgtagtag caacgataac
    17041 attttggtac tcgactgcgt ctaccagggc gtactctttg accaagccag cattgtcctt
    17101 caagcccatg atgtagagag gcttgtcatt gaggttgatg aggattggga aggttgcttt
    17161 gtaggatttc tcctgaacag caccttctgc tgattcacgg gctgattctt ctgtcgcaga
    17221 agccaagcta tacttagtga tttctcctgt tcgcatattt tcaaggatga aaccaagatt
    17281 actctcatcc gcattagccg acgtcacacc tgtgtagaga tagatgtcat taccgataga
    17341 caagtaatta tagccattgg tagtctgggt cacgtttttc ttggaaatca tggcattcaa
    17401 gaaaccgtcc ttgtacttgc cgttgtagtt gatttgctca atggtttcct ctgctggata
    17461 gatcctgtcc acccattctg gaacatctga taagctgtat tccttggttt ctccatttgt
    17521 agcatccaag ataatgactg aagcaggacg aggaacagca agtccaaatt gcttttggta
    17581 aaccgttgct acatagaaag gattgccctc atcgtccacc tcaaaagatg gagttttaaa
    17641 gattttggtc gggtacttca agcgcaggtg acgtttgaca tcacggttaa aatactccga
    17701 gtctgaatac ttgattggtg tcttcaagtc caccaaatcc gcatttccag ttaccatgtc
    17761 caccttaata tactcaccga ttcccttggc ttgattgtta aaccatttga tagggtctgc
    17821 atattctagt ggtgtgaccc gataaggttt cccatcaatt gtcaattggg tataggtatc
    17881 tgccgctacg tattgcgaca ccttatcggt tagggaaccc aagtagcggt ctccaatttt
    17941 ttcagcagta cttctatcta ggataggaac cttactggtg tcactcttag gaaattcagt
    18001 aaagtctttt tccgtaaccg tgactacatt ggcataattt ttagcctgaa acatgctgga
    18061 agttaccaag gaaaccaaag ctgccaagag aagaattcct ccaatcgaag ctaacagaat
    18121 tttccctaac cgattgattt tgaaaccctc aagatttaag gcagcttccg ccttaccgtg
    18181 gcgcacatga accgttttga caaggtttat ccccttgcca aagccaaata agattgccac
    18241 aactaacaaa tgcccacaga ggaagaagag aaattcccag ctggtcaggt taaggggcgg
    18301 taaaaagata taccaggtcg ttgcgataaa aataagttca aagactattc gtttcatttg
    18361 ctttcctcct aaatgattcg tccttccaag tgatccagct catgctggca aatctgagct
    18421 gggaagcctg tcaaggtaat ggtctgttcc tgccacttgc tgtcacgata ggcaaccctt
    18481 gtggtttcat aacgcttagt tgatctcaca cctaccaagg acaaacagcc ttcttcttcc
    18541 tcataggatc cttcaaagga cagaagcact gggttaaaca tgaccacggg aaccaagcca
    18601 agattaaaga taatcacgcg cttctgcacc ccaatcatat tggcagctag accaacacag
    18661 gtatcacgat ttgctaagag tgtatcctgc aaatctctgg caagataaag gtcctcctga
    18721 cttgccggct gagacacttg agataaaaat aagatatcct tcacaatttt cttttccact
    18781 tcctcacact cctcttgctt tttactatat tatagcaatt atagcacaaa agccgataac
    18841 tgcaaaccct ttccctcaac tgtagcaaaa agccatccga agatgacttt tttgctattt
    18901 aatttctgta taagttactt ccaagccacg cttaacagct ggacgattgg caattttttc
    18961 tgcccatttt actagatttt gataacttga ggcatccaag aattttgcag aaccttggta
    19021 aagatttcct tgaactaact gtccatacca agaccagata gcaatatctg caatcgtata
    19081 gtcattgcct gcaatataag gtttctgagc caattcctta tccaataaat ccaactggcg
    19141 tttcacttcc attgtaaaac ggttaatagg atattccaat ttttcaggag cataattgaa
    19201 gaaatgtcca aatcccccac ctagaaaagg tgctgcacct gcttgccaga atagccaatt
    19261 caaaacttct accttttcca caggattact tggtaaaaag gctccaaatt tctcagcaag
    19321 gtaaagaaga atatgagcag actcaaagac tcttacatct tcagtacctg actggtccaa
    19381 taaggctgga atcttggaat ttggattgag cttcacaaag tctgagccga attgatcccc
    19441 atccatgata gcaatcttat acaagtcgta agccgcttcc ttaaaaccag cttctagtaa
    19501 ttcttccaat aagatagtaa ccttcacacc atttggtgtt cccagtgaat aaagctgaaa
    19561 agcttgttct ccttttggca agttttgttc gaaacgggca cctgctgttg gtctgtttag
    19621 ccccgtaaaa gctccttgat tactagcttc atcctgccat acggtcggta attgatatgc
    19681 tgacatccga aacctccctt aaatcgcatt cttgtcaaaa ccgagtttgc gttgaataaa
    19741 cttaacgatt tcgacgatga taatcattga gaagcttcca gtcataacaa ttccccattg
    19801 tgacaagtct agtttggtta cgtggaagat tccttcaagc ggttctacaa cgattgttgc
    19861 catgagaagg ataaaggata ccaagatgga ccagttaaag gtcttagact tgaatgggcc
    19921 aactgtcaag atggattggt agacagactt gacattgtag gcatggaaga gctgaatcaa
    19981 accaagggtt gcaaaggcca tcgttagggc atctgcatga atagcatgat tgtcacccac
    20041 atgaactggg taagcaatcg caaggccata aacactcata acaagagctg cttggagtac
    20101 accttgataa atgatagaac tcaaaacacc acctgagaag aagcttgcct tgcgtccacg
    20161 tggtttatga ttcatgacac caggctccgc aggttcaaca ccaagagcga tagctgggaa
    20221 ggtatccgtt accaagttga tccacaaaag atgaaccggc tgcaagacat cccaaccaaa
    20281 caaggttgat aggaagatgg ttaatacttc agcagtatta gcagaaagta ggtactgaat
    20341 agtcttttga atgtttgaga agaccttacg tccttcttcc actgcgacga taatagtcgc
    20401 aaagttatca tctgcaagaa tcatatcaga agccccctta gaaacctctg taccagtgat
    20461 tcccataccg ataccgatat cggctgtttt cagagctggc gcgtcattga caccgtcacc
    20521 tgtcatggca acgactttac cttgtttttg ccaagccttg acgatacgaa ccttgtgttc
    20581 tggagacaca cgggcataaa cagagtattg accaacgact ttttcaaatt cttcatctga
    20641 cagttcattg agttcagcac cagttaaaac gtgaccttct gtatcgtttg cgtcaatgat
    20701 tcccaaacgt ttggcaatgg cttccgctgt gtcttggtgg tcacctgtaa tcataattgg
    20761 acggattccc gcttccttag ccacacgaac agcctcagcg gcttcaggac gttcagggtc
    20821 aatcatccca atcaaaccag taaaaattaa atcattttca agctcttcag aagtgagatt
    20881 ttctggaata ctatcgataa tcttataagc acctgcaagg acacgcaagg cttgatgagc
    20941 catttcagaa ttgtttgtat gaatgagatt tgtaaccttc tcatcaatcg gagcaatatc
    21001 cccagcctta tcacgaagaa gacaacgttt taagagttgg tctggcgcac ccttgactgc
    21061 tacaaggaaa cgaccatctg gcaatgggtg aactgttgac atgagcttac ggtcagagtc
    21121 aaatggcaat tcagctacac gaggatattt ctctaagaaa cctttgacat catagccctt
    21181 gtccaaggca tattggataa aggctgtttc ggttgggtca ccaatcaagt taccttccac
    21241 atcgattttc gtatcattgg ccaagacaac tgaacgaagt agtggcattt caagacctag
    21301 ttcaatatca tcagctgagt catgtagaac cgcatcgtag aagacttttt cgactgtcat
    21361 cttgttcata gtcagcgtac cagtcttatc agaagcgatg atttcagttg aaccaagtgt
    21421 ttcaactgct ggcaacttac gaacgatgga atgtcgtttg gccaaaactt gagtaccaag
    21481 agaaagaacg atggtaacga tagcaggaag tccttctgga atggctgcaa cggcaagggc
    21541 aacagaagtc aacaactcac caagtggatt tttcccttga atgaagacac ccactacaaa
    21601 agtaacaagg gcaatgacca agatagcata ggtcaagacc ttagaaaggt tgttcaagtt
    21661 ttgtttgagt ggtgtatcag tctcatccgc atcttgaagc ataccagcaa tatgaccaac
    21721 ttcagtgtac atacctgtat tgacaacaac acccatccca cgaccatagg ttacgtttga
    21781 gttttggaag gccatgttga cacggtcacc aataccagca tctgtcgcaa gctcgactga
    21841 caagtctttt tcgactggta cagattcacc tgtcaaggct gcttcttcaa ttttaagaga
    21901 gttggcttct atcaaacgta ggtccgctgg taccacgtca cctgcttcaa gggcaacgat
    21961 atcgcctggt accaattctt tagagtcaat ctctgccatg tgtccatcac gaagaacgcg
    22021 ggcaactgga ctagacatgg atttgagggc ttcaatagct tcttcagctt ttccttcttg
    22081 gtaaacacca aaggcagcgt tgatgataac cacagctagg atgataatgg catctgcgat
    22141 atcttcccca ccagaagtca cgactgacaa gattgctgcc gcaactagga tgataatcat
    22201 caaatcctta aattgctcga tgaatttgac caagattgat cgtttctcgc cttcttcgag
    22261 ttcattgtgc ccaaattcgg caaggcgctt ttccgcctca cttgatgaca aaccttgctc
    22321 ggtcgcatcc acagcctgca agacctcttc agggctctga gtataaaacg cttggcgttt
    22381 ttgttctttt gacatgtgtc tcctccttga cattgtgtgc aaaacagact ctctttctgt
    22441 catatctttt cacgacaaac aaaaagagac ctgttaatca taacaagtct cgctgtttaa
    22501 gatagggccg gaaagcatac ttttcagcat aaaattcgga atgacgacac tatcacaggt
    22561 ttctgccagc tactcccttg agtagtacca ttataccaaa ttttggggag ttttcaaaga
    22621 gtaaaaactg ccttatttga atttttcctt gaaaaccagt ataatggtag aatgctatgt
    22681 gactagaaag gaagttgaat gaagcaatct atctcaaatc tcaagttagc tgagcgtgga
    22741 gccattatca gtatttcgac ctatttgatc ttgtctgcag ccaaattagc agctggtcat
    22801 ctccttcatt catccagttt ggtggccgat ggttttaata acgtatcgga catcattgga
    22861 aatgtggccc tcttaatcgg gattcggatg gcgcgccagc ctgcagaccg tgaccaccgt
    22921 tttggtcatt ggaagattga agatttggca agcttgatca cttctatcat catgttctat
    22981 gtcggtttcg atgttctaag agataccatt caaaagattc tcagtcggga agaaacggtc
    23041 attgatcctc ttggtgcaac tctaggaatc atttctgcag cgattatgtt tgtggtctat
    23101 ctctacaata ctcgcctcag taagaaatcc aactccaagg cgctgaaggc agctgctaag
    23161 gacaatcttt ctgacgctgt tacctcactt ggaaccgcca ttgccatcct agctagtagt
    23221 ttcaattatc cgattgtgga taaactggtt gctatcatca tcactttctt tatcttgaag
    23281 actgcctatg atatcttcat cgagtcttcc tttagtcttt cagatggctt tgacgaccgc
    23341 ctgctcgagg actaccaaaa ggctatcatg gaaattccca aaatcagcaa ggtcaaatcg
    23401 caaagaggtc gcacctacgg tagcaacatc tacctggata ttacactaga gatgaatcct
    23461 gacttgtctg tttttgaaag ccatgaaatc gcggatcagg tcgagtctat gctggaggag
    23521 cgttttggcg tctttgatac cgatgtccat atcgaaccag cacctatccc tgaggatgaa
    23581 attttagaca atgtctataa aaaattgctt atgcgtgaac aattgattga ccaaggaaac
    23641 caactagaag aactcttgac tgatgatttt gtctatattc gccaagatgg agagcagatg
    23701 gataaagagg cttataagac caaaaaagag ttaaattctg ctataaagga cattcaaatt
    23761 acttccatca gtcaaaaaac caaactcatc tgctatgagt tagatggtat catccatacc
    23821 agtatctggc gtcgccacga aacctggcaa aatatctttc atcaagaaac caaaaaagaa
    23881 tagagaaatc ctttcatgag acgggatttt tctattcttt tatactcaat aaaaatcaaa
    23941 gtgcaaatta ggaagccggt cacaggctgt acttgagtcg gcaatgtgaa gccgacatag
    24001 tttgcacttt gattttcgaa tagtcttaac tatcaaattc actgagatac tcatagcgtt
    24061 cgtatttttc aaggagtgct tcatttttct catccaattc tttttggaga gtagccagct
    24121 taccaaagtc agagccgtta gcctgcattt cctcttcaat agcagcgata cgtttttcca
    24181 aggtttcaat atcaccttca atacttgccc actcctgctt ttcttggtag gtcatgcgtt
    24241 tcttgtcttc tcgaaccttg accacttttt ccttttcgac cttttgcact tgattggcca
    24301 tatctgtttc aaaagctttt tcatcaagat agtcggtgta atgaccaaag aaaggacgaa
    24361 tcttgccatc ctcaaaagcg agaatcttgg tcgctacctt atccaagaaa tagcggtcgt
    24421 gactgactgt taaaacggga cctgcaaaac cttgcaagaa attctctaag actgtcaaag
    24481 ttgcaatatc taggtcattg gttggctcgt ctaaaagaag aacatttggt ttttccaaaa
    24541 gcagtttgag gagataaaga cgttttttct caccccctga caatttctca atcaaggttc
    24601 catgcgtcga acgtgggaaa aggaactgct ccagcaactc tgcaatcgaa gtcgtagaac
    24661 cgccactggt cttgacctct tctgccactt cctgcaggta attgataact ctcttgcttt
    24721 catccaaacc ctcaatttgc tgagagaaat aggcgatgcg aacagtttcc ccaatcacaa
    24781 cttgtccttt agttggctca agacttcctg caatcaagtt gagcagggtt gattttccaa
    24841 caccattgtc cccaacgatt ccgatacggt ctttggcctg caccaagaga ttaaaatctt
    24901 gcaaaatagg cttgttttca taggcaaagg aaacagcctg aaactcgatg actttcttcc
    24961 caatccgact ggtttcaaag ttcatggtca agtctgtctc agcactactg cctgaaactt
    25021 cctttttcag atcatggaat cgattgatac gggcctgctg cttggtcgca cgcgcctgcg
    25081 gttgtctgcg catccaggcc aattcttgtt tgtagagttg ttcttttttg tgaagaagag
    25141 ccgcgtcgcg ctcatcctgt tccgccttta ggcgaacata gtcctggtaa tttccctggt
    25201 actcggtcaa gcctgcacga tccaactcga aaatccgtgt tgacaaagcg tctaagaaat
    25261 aacgatcgtg agtgataaaa aggacggtct tcttagaatt tttcaaaaag agggtcagcc
    25321 actcaataat cgcaatatcc agatggttgg tcggctcatc caaaagcaag aggtcgtggt
    25381 tgccaagtaa gacttgtgcc aactgtaccc gtcttctcag accacctgac aattccccaa
    25441 caggagtaga taagtcttga atgcccaatt tgctaagaac ggtcttgacc tgactctcga
    25501 tttcccaagc ttggagagag tccatctctg ccatgacacg ttccaaacgc gcctgcttgt
    25561 cctcactata gtcgagcata atcaattcat actcacgaat gagctggatt tccttgagtt
    25621 cactagatag aaccgtatcc aaaactgtct ttctatcatc aaaatcagga tcctgagtca
    25681 agtaaccaat ctggtaatca tttttagctg aaaaaggact gacatcccca tcaaatccag
    25741 aaacaccaga aaggacgtcc aaaagggtgg tcttgccagt cccattgaca ccgattaaac
    25801 caattctgtc taagtcatgg ataataaagg aaatatccct aaaaacggtc ttgtcaccaa
    25861 cggatttact tagtttttca acgataaaat cactcatttt ttctccctca ggtaagcatg
    25921 gatggcttca cgattattct ccaattctcc atcgacaatg gcaaactcaa tctctgttaa
    25981 aatctctccc aagtctgggc ctggctgata gccatattcc ttgatcaaaa tatcgccatt
    26041 aatctgaatc tctttcttgt catggatagt caagctttgg tatttttctg tgatggcttg
    26101 tgggttgact tcttttcctt gagcttgacg aagattttca gcctgtaaaa gcaaatctat
    26161 gtcaaagcga taacaatctc gcttgctcaa ttctccattt tcacgcagag ccaaaataat
    26221 cagcaaatcc tgaacttgct tggcaaactg gcgtgaggtc ttccaagatt tcaaaaatga
    26281 ctgcgcattt tcaatctcca aagcccatag taaagccgcc caggcttgtt cagaggattc
    26341 aaaagtaaaa tcagtctcca aatcaaacag tctgttgagc ttgtcctggc tagatgccat
    26401 atcagggaga tagtcataag cttgactctc aatcatggaa gccaagcccc ttctccaaaa
    26461 tggagccagc aagagtttat caaactcgac gaaggtacgc tctacagaaa ttttctccaa
    26521 aagcggcgtc aaggtcttca tagctttaaa tgtttctggc tcaagtgcaa aaccaagact
    26581 agcctgaaaa cggaaaccac gcataatccg taaagcatct tcgttgaaac gctcactagc
    26641 cactccaact gctcgcaaga cttgcttttc caaatcttct aaaccatgga acaagtcaac
    26701 gatttctcct gtctcatcca aggcaaaggc gttgactgtg aaatcacggc gtttgaggtc
    26761 ttcttctagc gatcgtacaa aggaaaccgc actgggtctg cgatagtcca catagacatc
    26821 ctctgtccga aaggttgtta cctcatactc ctcatcccca tctaagacca agacggttcc
    26881 atgctcgatt ccgatatcgg ctgttcgcgg aaaaatctgc ttggtctctt ctggataaga
    26941 agacgtcgca atatccacat cgtggatagg gttatggaga agggcatctc gaacagagcc
    27001 cccaacaaaa taagcctcaa agcctgcttc tttaattttt tctaatactg gtaaagcctt
    27061 ctgaaattca gaaggcattt gcgttaatct cataataagt gttctaatcc atagacaagc
    27121 tcatgacgct tgacaacttc tttaattccc aaattgactc ctgtcatgaa ggagatgcga
    27181 tcataggagt catgacggag ggtcatccct tctccctgat tgccaaagat gacttcctga
    27241 tgagctacca agcctggcaa acgaactgag tggatgcgca taccatcaaa gtcagcacca
    27301 cgagcaccag caatcagctc ttcctcatct gctgcgcctt gctgaatgga ctctcgaacc
    27361 tctgccatca actcagctgt tttaatggct gttccactcg gagcatcctt tttcttgtca
    27421 tgatggagct caataatctc cacatttggg aaatatttgg cagcctgcgt cgcaaattgc
    27481 atgagtaaga cagcacccaa ggcaaagtta ggggcaatca ggccacccaa gtcttgggca
    27541 cgagaaaatt cttttagctc tgcaatttct tcactcgtga aaccagtcgt tccaactact
    27601 ggagcaaagc cattttcaag agcaaaacgt gtattttcgt aggcaacagc tggagtagta
    27661 aaatctaccc agacatccgc ttcaaaacca gctaaatcag ccttatcctt gaaaacagga
    27721 ataccctgcc attctgactc agactcaaaa ggatccaaaa ctgctaccaa gtccaagtct
    27781 ggatcagtca ataccatctg acaagcagcc tggcccatct ttcccttaaa accggcaata
    27841 attactcgaa tactcatctc tactcctgtc taagatacaa agtccgtaag aacacaaagt
    27901 gaaaatagga attccaatca agaagtgtct acttcttgga agaactatct ttttcacaca
    27961 gggttccagg cgtgttcaat tatcaagata caaaggacct tagctgcctc tgaaaaatag
    28021 ggaatggcac tgactttcca cgaaaggcaa gacaggcatc ttttttcaag aggcaggtag
    28081 tccgtgttca atttctaaga tacaaggcat cttaactagc ctagaagcgc caactaaatc
    28141 actggaatat aacccagagc aatacttcct gctcctaggt gcgttccaat gacactacca
    28201 aatgtagcaa gtgaaacatc cgaacccaag ccaaaatcaa gcaagtgctg acgcaattct
    28261 tcagcctttt caggagcatt cccatgaatg acaatgaccc ggtattgacc tgaagccgtt
    28321 gtttccttga taatttcaat taagcgcttg gtggccttct tttcagtacg aactttttcg
    28381 taaacttcaa tcacaccttg atcgttaaaa taaaggattg gcttaatgct aagcaaattg
    28441 cccaaaatgg cagccccatt tgaaaggcgt ccacctttta ccaaatgatc caagtcatct
    28501 accatgataa aggctgacgt acggctgatt tgaatggcta gcttatcctg aatgctggca
    28561 aaatcatcgc cctgatcacg ccaattaaag acgctttcaa ccatgatgcc taggggagca
    28621 cttgtaatca aagtgtctgg gaaagcaatg gttaagccct catagtcatc gaccatatac
    28681 tggatatttt ggtaaaaacc tgaaattcca gaagatagga aaagccccaa ggcatgtgta
    28741 tagccttgtt ctttgagcga agttaagatc tcacctaact tggcaatact tggttgactg
    28801 gtcttaggca attcagaagc ctgagccatt ttttggtaaa attcctcagc agacagattg
    28861 atgccttcga catattcctc accatcaata ttgacaggaa tatccaagac aaacaagtct
    28921 tctctttgca aggtctcttc actgagataa gcagaggaat ctgtgaaaac agctaatttc
    28981 atattagaac tccaaattaa ttcctggtaa gtctaatgca atttcagtca cttcgtaagt
    29041 caaacgattg agcatgttca aacatggacg agccaaggtt tccacctctt cttggttcaa
    29101 ttcacttggt tcattgacaa tacggccatc gatatggttt acttgtgaga ttgttccact
    29161 aatgacaaac ttatcaaata caatcataaa gctcaagatg acaatcaagg aagtcacttg
    29221 attttcttgg tcatgttgga gcaattggaa attcacatcc accttggttt caggagctcc
    29281 attttcattt tcccattcaa aattacgcgc atcaaaatga tactgactaa caaattcttg
    29341 ttcacgttta agattcatgt ctttctccat cggctacaat attataagct attgtaccat
    29401 aattttttat tttcatctag ttttctagga tttagtcaat cccaatttca gcacgaacta
    29461 catctgtgat agtatcaaca tagtagtcca cttcttctgt tgtaggcgct tctgccataa
    29521 cacgcaagag gggctctgtt ccacttggac gaacaaggat acggccgttc cccgccattt
    29581 cttcttccat cttctcgatg atggccttga tagctggcac ttccatggcc ttttccttca
    29641 tgacgttttc cactcggata ttaactaatt tttgtggata aatcgttact tctgccgcca
    29701 actctgataa gctcttacca gtttccttca taattttagt caattgaact gctgataatt
    29761 gaccatcacc tgtggtattg taatccatca agataacgtg accagactgt tcaccaccaa
    29821 ggttgtagcc tgattttctc atttcttcaa caacgtagcg gtcaccaact gcagtaactg
    29881 ccttgttaat accttcgcga ttcaaggcct tgtggaaacc aaggttagac ataacagttg
    29941 tcacaattgt attttgagcc aattgtcctt tttcagaaag gtattttccg atgatgtaca
    30001 taatcttgtc accatcgacg atgtcaccat tctcatcaac agcaatcaag cggtcactgt
    30061 ctccatcaaa ggccaaacca atagctgacc cactttcttt gaccacttct tgaagggctt
    30121 ctggatgtgt tgaaccaaca ttaaggttga tgttaagacc gtctggtgtt tccccgataa
    30181 ccgtcaattg ggcaccaagg tctgcaaaga tttgacgggc actggtagaa gctgctccat
    30241 tagctgtatc caaggcaacc ttcattccat caagaggagt tccagttgaa acaaggtatc
    30301 cttcatactt acgcaagcct tctggataat ctaccaaaat tcctaagcct tctgcacttg
    30361 gacgaggaag agtgtcttcc tcagcatcta gcaaggcttc aatttctgct tctttttcat
    30421 catctagttt gaagccatca ccgccaaaga acttgattcc gttatcaagg gctgggttgt
    30481 ggctagcaga aatcatgaca ccggcacttg ctccttcagt ttcaaccaag taagctactg
    30541 ctggtgttgc aaggacacca agtttgtata cgtgaatccc tactgaaagg agacctgcca
    30601 ccaaggccga ttccagcatt tcccctgaaa tacgtgtgtc acgtcctaca aagactttcg
    30661 gcgcttccgt ttcatgttga ctaagaacat agcctccaaa acgtcctagt ttaaaggcta
    30721 attctggtgt tagttctagg ttagcttctc cacggactcc atcagtccca aaatatttac
    30781 ccattgttat aaaatccttt tctattttta attcgttttt gaactagttg ctttcgttga
    30841 cgaagatgtc tccgatgaac tgcttgtact tgaatttgat gtgcttgaac ttggtgctac
    30901 tggttttgta gtcaccttca ttattgtatc aaacggagtg ataactgccg gtaagacaac
    30961 accattgcgg tcgattgcct gcaaaggtac tgaaccactg taattacctg ttatacgttc
    31021 gctagttggc aagacagcga taatcttatc aattctatcc aatgtctctt ggtcactcgt
    31081 aatcgacact tctttatctg acaccatgac attttcaatt tgtacccgac tatcaatttg
    31141 actagggtca atctctggta caatctttac cttatccttc tgagccttct taccaatctt
    31201 gactgtaatt ttttgcggag tcgccacagc ggtcagccca ttgggtaaat cttcaatgct
    31261 caaaggaact tcaatcgttc caacaccggc atctgttagg tcagcagtaa ccttgaattt
    31321 acgtgtactt tcttgcattt cactagctag cgataggcga tttgcaccag tcaagaccac
    31381 tgatacttct gaagcaaaac cgctaataaa atacttatca ctattatagc gtatgtcaat
    31441 agggacattt gttactgtat tagtataggt ttccgttttt acctgcctag cactggtact
    31501 gttttgaaaa ttcgtcgccg tagcatagac aaataagaca caagcaaaaa agagtgagga
    31561 tatgatatat aaactatttt ttttcatgtt tccatcctcc tagccatcgt tctttaaaac
    31621 taagacccac ttcctctttt ggaagtaaga tttcacgtaa ttctgtttca aattcatcaa
    31681 gtgttaggtt gtgcttaaac cttccattat aggttatcga aattcctccc gtttcctctg
    31741 atacgacaaa agtcaaggca tctgagactt ctgataaacc gatagccgcc cggtgtctgg
    31801 tcccaaattc cttggaaata cctgtgtttt ttgtcaaggg cagataggca gacgtcacag
    31861 cgatacgttc ttctttgata atcaccgcac cgtcatgtag gggagtgttg ggaataaaaa
    31921 tgttaatgag aagttctgca gaaatcttag catccaaggg gattcctgtc gaaatatact
    31981 cctgcaaggt acgtacacgc tgaatagcaa ccaaggcccc gattttacga ggactcatgt
    32041 attcaacaga cttaacaaag gcacgaatca tctgttcctc agcactaata ggggcattgg
    32101 aaaagaaatc tgtcgctctt cccaaacgtt ccaaaccagt ccgaatctct ggagagaaga
    32161 taacaaccgc cgcaataacc ccataagtaa taatttgatt gattaaccaa gaaatcgtag
    32221 tcaaaccaat catatttgca aggatttgag ctaaaataaa taccaaaact ccacgtacca
    32281 aaatcataat cttggttcct gcaatagctt ttgtaaaatg gtataaaata taagcaacaa
    32341 tcaaaatatc aatcagattg atagctatcg tccatggact tgcaaacaaa ctggtccaat
    32401 attgcagatt ggataattgt tgaaaattca tccctgatat cctccctatc aaaacacttt
    32461 cgtcctatta taccattttc gggcattttt ttccctatcc tagtccattt tacattgaac
    32521 aaaaatatga taaaataaac tgactagaaa aaacaaagga gaaactatgt ctcaactcta
    32581 tgatattacc attgtgggtg gtggtcctgt cgggcttttt gcagcctttt atgcccacct
    32641 acgccaagcc aaggttcaaa tcatcgactc tcttccccag ctaggtggac aacctgctat
    32701 tctctaccct gaaaaggaaa tcctagacgt accaggcttc ccaaacctga ctggagaaga
    32761 gttgactaac cgcttgattg aacagctaaa tggatttgat acccctattc atctcaatga
    32821 aacggttctt gagattgaca aacaagaaga agaatttgcc atcacaactt ctaaaggaag
    32881 tcacctgact aaaacagtta tcatcgctat gggtggcggt gccttcaaac cacgtccgct
    32941 ggaacttgaa ggggttgagg gctatgaaaa tatccactac cacgtttcta acattcagca
    33001 atacgctggt aagaaagtga cgattcttgg tgggggagac tcggctgtgg attgggcttt
    33061 ggcttttgaa aaaatcgcac caactaccct tgttcaccgc agagataatt tccgtgcctt
    33121 ggaacacagt gttcaagcct tgcaagaatc atctgtaacc atcaagacac cattcgcccc
    33181 tggccaactc cttggaaatg gaaaaacact tgataaactt gaaatcacaa aagtcaaatc
    33241 tgatgaaact gaaaccattg acctagacca cctctttgtc aactatggtt tcaaatcttc
    33301 tgtcggtaac cttaaaaact gggggctcga cctcaaccgt cacaagatta tcgtcaacag
    33361 caaacaagaa tccagccaag caggtatcta tgctatcggt gactgctgct actatgacgg
    33421 aaaaattgat ctgattgcga caggcctcgg agaagctcca actgctgtca acaacgctat
    33481 caactacatt gaccctgaac aaaaagtaca accaaaacac tctaccagtt tataaaaaag
    33541 aaccacgagt cacataggat tcgtggtttt ataattcatc cgctatctta ttgatttttc
    33601 tgagtctgtg attgacacca cttttggtca gaggggtgct gagactatct gctaactgct
    33661 ggatagagta gtctgggtgc tgaatcctca gttgcgccac ttcctgcaaa tctactggca
    33721 aattttctaa gcccatgata tctttgattt tactgatatt gttaatggtc ttcatgctgg
    33781 cagaaactgt ccgagcgata ttagctgtct cggcattatt agcccgattg aggtcgttac
    33841 gggtttctcg caaaatctta acccgctcaa aatcatcacg tgcctgcatg gctccgatta
    33901 ctatcaagaa gtccataatg tcttctgctc gctggagata ggtcacagcc cccttcttgc
    33961 gctcaagcac cttggcatcc agtaaaaact gttggagaag ggaggcaatt ccttgcgcgt
    34021 ggtccagata aacagaactg atttccaact ggtacttgcc tgactcaggg tcacgaatgc
    34081 tcccatttgc caagaaagcg ccacagagat aggcacgacc tgcttcctca tccgataaaa
    34141 tcgcctcatc aatacctgtt tccaggccaa agaaagagtc tgccaagtgc aaatcactta
    34201 acaaatcctg caccttttca tctgtaaaaa cggtatagac gcgattcttg cgaagattgc
    34261 tacgttggtg gtgacgaatt tcagatttga tttcatagaa atgaagaaag gactcataga
    34321 ggtgacgggc cagtttggca ttttctgtca caactgacaa agtcaagccc gaagtcgaga
    34381 gaccgatgct accagacatt ttgataatgg cagataattc atgccagctc agatggtgtt
    34441 ggcccaggat ttcttctttt actgctactg tgaaactcat tttttcacct gtataatgcg
    34501 catcaactcg tccacaatca aatctccatc gtggaaggca ccgccatttt ccagacgaag
    34561 gaagttagat gaaatcacgc gcgaaacttg cttacaaaga cctacaaaat cgtgttccac
    34621 ttgcactaag tattcatcaa aacggttgga attcatgtat tcctgaggca ctttttcaat
    34681 attcaccaag acagtgtcga taaaagggcg accaaggtga cgatgcaaga cttccacgtg
    34741 gtcgctatct gtaaagtgtt ccgtctcccc acgttgggtc atgatattgc agacataggc
    34801 aatttctgcc ttggtttcca aaagagcccg cccaatttcc ttaatcacga tattgggcaa
    34861 aatagaggta aagagggaac ctggccctag gacaatcatg tcactttcaa ggatggtctg
    34921 cactactcga cggctggcca gaggcgtatc atcgtttagg gcattggtca catagacatt
    34981 gtcaattatg cctcgatggt ctacaatatg actctctcca gccacttcta tcccatcctg
    35041 aaagactgca tgaagggtca aaggatggtc actggaagga taaattttcc ctgttgtatg
    35101 gaaaaatttg ctcaataact gcatggcatt ataggttgaa ccctgcattt ctgacaagcc
    35161 agcaatgatg agatttccca atggatggcc agcaaaggct ccggcatcct cagagaaccg
    35221 atactgaaag accttctcat aaaacttagg catatccgac atggccacaa ggacattacg
    35281 aagatcacct ggcggtgtca actgttgcat attttttcgg agttcacctg aagaaccacc
    35341 atcatctgcc accgtcacga tagctgcgat ttccacatct ttttcccgca gactttttag
    35401 aatgacggga attccagtcc ctccaccaat caccgttatc tttggttttc tcatgaacgg
    35461 tttaccgttt cctttctgcg gtctttgtcg cgatgccctt cattaacaga ccaattcttg
    35521 gataagtcct gcaccaagcg tttagcaaat gccacactac ggtgttgtcc acccgtacat
    35581 cccatggcaa tggtcaaaac ggacttacct tccttttggt aacttggcag aatcggctca
    35641 atcaaggcca ataaatgttg ataaaagtct tctgactcag gatggttcat gacataatca
    35701 taaacaggtt catccacacc cgtttggttt ctcagttcta gtaaataata gggatttggc
    35761 aagaaacgga catcaaagac caagtccgca tcaatcggga ttccatactt aaatccgaaa
    35821 gacatgactt cgatacggaa agactgggct tgttcttggt ctgaaaactg ctctgcaagg
    35881 gttttgcgca gctcacgtgg agtgagttca gtcgtatcca ccacattttg gctcatattt
    35941 ttcaaaggtg ccaagagttc acgttccaac ttgattccat ctaaaatacg accgtctgct
    36001 gctagtgggt gactccgtct ggtttccttg taacgagcga ccaattcctt atcagccgca
    36061 tccaaaaaga ggattttgaa atccaaacca tcttgatttt ccaactcatc caaaacagct
    36121 tgaatctctg aaaagaaaga acggctacgc atatccacta ccaaggccaa cttaggattg
    36181 tcttccttaa tttcaaccag ctgcaaaaac ttaggcaaga gagctggcgg catattatca
    36241 atggtgaaat aacctagatc ctcgaaggac tgaatggcta cagttttccc tgcgccactc
    36301 atccctgtca caatcaccaa gtgaagttgt ttctttgtca tctttttctc cttatatcaa
    36361 aagaagtttg gcaacaccaa acttcaacta gcttatccaa tctctgcgat gacttcaatt
    36421 tcgactttta catcacgagg aagacgagct acctccacag ctgaacgagc tgggaattcc
    36481 tctttgaagg ccgtttggta aacctcatta aaaggaacaa agtcgttcat atcgctcaag
    36541 aagcaagttg ttttgacaac atggtcaaag tctgttcctg cttctgccaa aatagcaccg
    36601 atgtttttca agacttgctc tgtctgttct tggatattct ctcctacaat ttccccagtt
    36661 tcaggggata ggggaacttg accgctagca aacaaaaggt tgccaacgat ttttccttga
    36721 acatagggtc cgatagcctt tggggcctta tctgtatgaa ttgtttttgc cattttcttt
    36781 tcctcacaat ttttctaaga ttgcatccca agcctcatcc atccctgcct tactgacaga
    36841 tgaaaagagg atgaaatcgt cactcgggtc aaagtttaat ttctttttga ttgctgattc
    36901 atgcttgttc catttaccac gaggaatctt gtccgccttg gtcgccacaa tgatgactgg
    36961 aatctcataa tacttgagaa attcgtacat ctgcacatca tctgctgacg gggcatgacg
    37021 aaggtcaact agactgacaa ccgcacggag attttcccga gtcgttaagt actcctcaat
    37081 catgcacccc cacttttcac gttccttttt agaaacacga gcatagccat aaccaggcac
    37141 atccacaaag cgcatcttgt catcaatgtt aaaaaagttc aggagctggg ttttaccagg
    37201 ttttcctgat gtacgggcga gattcttacg gttcaacata gtgttgataa agctggattt
    37261 accaacattt gaacgccctg ctagggcaat ctctggcagt tcatcctgcg gatagtggga
    37321 cttattagct gcactgagca agatttcagc attgtgtgta ttaagttcca tagtcacctc
    37381 taggctgttt ctaggatcgg tttatccgtt ccatcgacag tttctttagt gatgcgaacc
    37441 aatttcacat tttcctgact cggcacctca aacatgacat ctagcatggt ttcttcgatg
    37501 atggagcgaa gtccacgcgc ccctgtcttc cgttcgattg ctttattagc aatctcttga
    37561 agggcttcgt cgtcaaattc caactcaaca tcatcataag aaagcaaggt ttggtattgt
    37621 ttcaccaagg catttcttgg ctctttcaag atgcgaacca agtcatcaac ggtcaattgc
    37681 tcaagagccg caaaaacagg caagcgtcca atcaactcag ggataatacc aaatttttga
    37741 atgtcttcag cgatgatttc ttgcatatat gagctgtttt catcaatcgc cttattgttt
    37801 tgaccaaatc cgatgacttt ttcacccaga cgttgtttga caatttcttc aataccatca
    37861 aaagcaccac ccacgatgaa gaggatattt tttgtatcca cttgaatcat ctcttgttgt
    37921 ggatgtttgc gtccaccttg aggcggcacg ctagcaacag ttccctcgat aatcttgaga
    37981 agggcttgtt gcaccccttc accagaaaca tcacgtgtga tagacacatt ctcactcttc
    38041 ttggcaatct tgtcaatttc atccacatag ataatgccac gctctgcacg ttcgatgtta
    38101 aagtcagcaa cctgcaagag tttgaggagg atattttcca catcctcacc cacataacca
    38161 gcctccgtca gagctgtcgc atccgcaata gcaaaaggca cattcaagct cttagccaag
    38221 gtctgggcaa ggaaagtttt ccctgaacca gttgggccaa tcatcaaaat gtttgacttc
    38281 tgcaaatcta catcttctga ctcttcgcgt gtatcgtgga aattgatgcg tttgtagtgg
    38341 ttataaaccg ccactgccaa ggcacgcttg gcacgatctt gaccaattac atagtggttc
    38401 aagatatgga ggagttcaat tggttttggc acctcagaca agtctgccaa gacttcctca
    38461 accaattctt ctcgaatgat ttcctgagct aactccacgc attcattaca aataaaagca
    38521 ttgttgccag caattatttt ttgtacttct tcttggtttt tgccacaaaa tgagcaataa
    38581 accatcatat cattttttct atttgtagac atgatttcct tccattctat actgtcattc
    38641 tatctaaaat aaggtcatgt aaaaagcatg aatactattg accagattgg taaaggcatt
    38701 taaccaaagg aggatagaaa gcccgtaacg ctttttacga aaagcctgtg ctcctgccag
    38761 aaagcagatg aaacacagaa aagccgtgaa tagaccaaat aaactccgtt ccattagact
    38821 tcctttctct tgcggtattg gatggtaaaa tcataaggat tcttctcatc tttggcgtaa
    38881 aatttgcttg aaactgtctc aaaaagagac aagtcaagct cttcagggaa ataggtatct
    38941 ccttccaccc gagcatgaat gtgagtgaca atcacttcat caaggtaagg tccaaaagcc
    39001 tgaaaaattt gcttcccacc gataatgtag agattctttt cttgaacctg ataccagtca
    39061 agaacagact ggacgtcctg aaaagtagca accccatcta tcttttcttc cggattacgc
    39121 gtcaaaatca aggtttcccg ttttggaagc aagcgacgcc ccatcccatc aaaggtcaca
    39181 cgccccatca agatagcatg attcagagtt gtttctttaa agtgctgcaa ttctgctggc
    39241 aaatgccaag gcagacgatt ttccttacca atcacaccct cttcatcctg ggcccaaata
    39301 gctacgattt tcttagtcat gcttccatcc ttttcactga tagtactatt ttatcaaaaa
    39361 actcaaaaaa agactggttt ggaatagctt acaaaataga aaaaatctgt aagaaatttc
    39421 ctacagattt atctatgttt ccttatttct tacaaaccag gtgcttgtcc aagttcggct
    39481 gcaagcatcc aaattgtttt atctgtttca gttttagcac ctgcaaagat accgtttgtc
    39541 acatcgtcac cttcttcatc agtgacatcc aaaccttttt ggaaaagttc tgacaagtaa
    39601 cggtagataa caagaacacg ttccaagctt tcttcaacat tacggtattc accagcttct
    39661 tcttcgattt cactattttg aaggaactct gtcaatgtag agaatgggct tccaccgagt
    39721 gtaatcaagc gttcgctgat ttcatccaat tgaccgtcaa gagcttccat gtactcatcc
    39781 atttttggat gccatacaag gaaaccacga ccatgcatat accagtgcac ttggtgcaaa
    39841 gcaacgtgag ctacatacaa atcagcaaca gcttggttca agacttcctt tgtttttgcc
    39901 aatgctactg gcgctgcttt tgtcaatgat gttacgtctt ttactgcttc ttttttcaat
    39961 tctaccattt tctttacctc attcattatt ttttgtaacc acttcttaat gattactatc
    40021 ttagtatact actaaggaaa accaatagca agacaaagaa cttatatgag aaaagttgca
    40081 atagactgat aatttaagaa ttctttagaa gacaaaaaag aaggttggat gcttatagtc
    40141 aatggaaatc catcagcaaa ctaggaaact aaacccagct tgaccaaagc acaagttttg
    40201 aggtcgcagg tagaactgac caagctgatg tggtttgaag aaattcccca atactataat
    40261 agattgaaat aagatgaaac caagtcgact aggaaaggta aatgaatttc taaaatgttt
    40321 taccaatcac aaggtatttg tctatattca atctactata taagcttcat ttccgagcaa
    40381 gatcttgaat ttactcgagg atgaactatc ataaaacaaa gaaaaagagg gtgctactat
    40441 cacatccctc tttcaaatca tcgcttaata ccaaacgctg acatctacgc gcccttggat
    40501 tcctttcatg tattcagaag aggtatattg ccaacctttt tttcctgaat aatgagggtt
    40561 ttcccattct aaagcattcg tataggccgc tacccagttt acatgtttta aaatatctgg
    40621 gtgttttaaa cgcgtctgta ataaactacg atagctatag acatatacat tttgataacc
    40681 cgcctgcttc atcgtgtcca tgtacttgtt gatgatttta acccaagtgt ctgtatcact
    40741 tggagctctc ttgctcttat ttacatattc ccagttctca acatcataat agatagggta
    40801 agacaggttc atattgtatt tctttataag ttcaatggtc tgtttagcgt cattctcagc
    40861 atcggtctca ttttcagcat aggtatagag atagacacca taaggaattc ccagacggtt
    40921 taactcctta atgttatgcg ccaattcctt gtcttcttta ccgctataac ctagacgaac
    40981 aatgacacca tctacctcgt tctcatcaat aacctttttc caatcattga tacgaccatt
    41041 gtgctcacta atatcaatga ttttcttagc atgttcggtt cccacttgtt cttctctatt
    41101 attaaagaaa tagcgtttac catctctgtg aacccagccg acattcaaat ctttcttttc
    41161 tttgagttca ccagaggccg caaagatata gcgagcacca tccataatca cttcgtcagt
    41221 cgccatggca ccacttccag tcaaatagta gttgccttgc cactcattcc gagccatata
    41281 gccccacttc ttgaaatagt accatttgcc gcccactttt tgccactctt ggttagcata
    41341 agttccatcg gattttagat aaaaataagc agaataagct ggatcataga gccattcatt
    41401 ttgtatcata gctccttgac cattcaagaa ataacttcct tgccattggc ttttagccat
    41461 gtaaccccac ttcttgaagt agtaccactt atttccaatc aattgccatt cttgatgagc
    41521 ataagcccca tctgacttga gataaaacca actcttgtaa ttactgtcat agatccactc
    41581 gttttgggcc atatatccac ctgatttcag gtagtaattt ccatgccatt cattctgcga
    41641 gtaacgacca tctgaattaa tataaaacca agccttatag tagttgtcaa aaatccattc
    41701 actttgagct ttagaaccat catcctttac ataataatcc ccatcccagt gggcagcagc
    41761 attagtctgt acttctgcgc tcgtttgcgt tttattagaa gactgatttt gctctgaact
    41821 acttgaagct gtcgttgtat cctgcaaagt ttttgctact tcagtttcat ttgcagcgac
    41881 atgactagtt gccaagccta gtaaacagat acttgctaat ccaattttta ttttcaatct
    41941 ttcctctcct ataaaaaatg taacagacat ctgaatgctg ttccacctag cttttgctac
    42001 ttactgatta ttttacaaag tcaagcaaag ccaagaagct ttcagcttca agtgacgcac
    42061 cacctacaag ggcaccgtca acgtctgggc aagccatgta tgaagcaaca ttttcaggtt
    42121 taacagaacc accgtattga acacgaactt tgtctgcgac ttcttgacca aagtcagcag
    42181 ctacaacgtc acgaacaact ttacacattt tttgtgcatc gtcttgtgaa gctgatttac
    42241 cagtaccgat agcccagatt ggctcataag cgataactga ggcagcaact tgttcagcag
    42301 tcaatccagc caatgcagca gatacttgag cacctacgaa ttcagcagct ttaccagctt
    42361 cgtaagtttc aagtgattca ccacaacaga tgattggaag cataccgttc gcaaagattg
    42421 cttttgcttt tttgttgata tcttcatcag tttcatggaa gtagtcacgg cgttctgagt
    42481 gaccgataac aacgtagtca gtaccgattt ctttcaaaac ttgtgggcta gtttcaccag
    42541 tgaaagcacc tgcattttca aagtagcagt tttgagcagc aactttaagg tttgagcctt
    42601 ttgcaacagc aagaacagtt gtcaaatcaa gagctggagc agcgatacct gcttcaacaa
    42661 gatctgatga aggaagtttt gatgcaactg cttcaacgaa tgctttagct tcttctggat
    42721 ttttgttcat tttccagtta ccagcgataa atggtttacg tgacatttca catacctctt
    42781 ttttcaattt attttctatt tattctatca caattataca cagcttgcaa atcttactcg
    42841 gatttcaagc ctacttgcat ggattaatcc ttccagagat ccatggcatt tatgaaatct
    42901 gcagatacct gtgtcaactg aggattgctt gcttctcttt ctgctctctt ggcctcaatt
    42961 tgagccacac tcttgattcc ttcatggcgc cagtttctca aaatcgcctg aatgtacttc
    43021 cagtttggtt ttccattcaa aacagcttca cgaagagcct ccttaatcaa gtcagcactg
    43081 gttccatctt cctttagtgt cttggtcaaa tcctcaatct caaaaggcgt caacaatcgt
    43141 cccaactcct gctggaaggt ttccaccaaa tccttgagct ggttttgtgg tgttagctgg
    43201 tctgaacttg aatgaactgc tccaaacagg tcatccaaac gttccaaggc caaactagca
    43261 tcaaagagca attcaatttc gccatttaat tcgatagtac gatactggag cagtcccctt
    43321 tccgtcagat tagaaatgga ctggttgaca tccgaaattt ccttgccaat cctttcagca
    43381 atctggcttg gcgatatttc ttctaagcct gtcgtatttt gcaaatagaa aaattgccaa
    43441 accagaaaat cgtcgctaga aggaaagagt tccttaaaat gcaagagcag ggcactcggt
    43501 aaaaccaagg tacctgattt aaaagcgtct aaatatgtca taattcctct tataaatacc
    43561 tatatgcaga tgcatcatct tctatctttc tccagtcaaa aggcgtctcc tgatagaccg
    43621 catggtttac ccaattactg aaaaagaggg ctgcagatga agaccaacaa agacaaggta
    43681 cctgattaac atcatcatct ttaaagtaat tttctggaat atgtgggtcg aaacctgcat
    43741 cacgatctcg aaaatactct tttgccaagg tatcacggtc atactccaaa tgaccaaaac
    43801 tataaatttc tcgtaaatca cgactggcca aaatagaaac cccaacctga ggtccttctg
    43861 ataaaatctc gagattggtc ttgtttaaga cctcttcctt agaaatctcc gtgtgccgtg
    43921 aatgagggga tacatagcta tcatcaaagc ctctaaatag aaggtgaccc tcttttaggg
    43981 tgtcctgagg ataaatacct gatagcttac tgtccatctg gtatttttct acaccatagc
    44041 gcagataaag cccagcctga gccccccaac agatatgaag ggtcgaatag acatgagtct
    44101 tagaccactc aagcatctgt ctaaattcct cccaatagtc cacttcctca aatggtaaat
    44161 gctcaactgg agcacccgtg atgatcatcc catcaaaata ctcatccttg acttcaggaa
    44221 aagttttata gaaggtctcc atgtgctctg aacgagttgt tttagaacgg tggctctcca
    44281 tatagagaaa atcaatatcc agttgtaggg gtgtattagc caagtggcgc aacaactggg
    44341 tctctgtgac cattttctgt ggcatgagat ttaaaattaa aatcttcaaa ggacggatat
    44401 cttggtgggc agcacgttga tcatccatga caaagatatt ctctgtccgt aaaatctcaa
    44461 cagctggcaa ttttttatca attcgaatcg gcataaccct ctcctaactg tactctttca
    44521 ggaataattg catatgtttg aagacaaatc tcaaacactt agtcctttta ttatactgca
    44581 agaagatata gttttcaatt atagtttttc tctaactagt tatagtctat ttttatatcc
    44641 tagtgtagag aaaacagccc tagggactgt tttcattaat aatgcataag aactttgtag
    44701 tcgtagtcac caattttttc acggccgttc aattcatcca attcaacaag gaaggcacaa
    44761 cctgccataa caccaccaag tttttcaatc atctcgatag ttgccttaac agttccacct
    44821 gtcgccaaaa ggtcatctac aataagaaca cgttgacctg gcttaatggc atccgcgtgc
    44881 atagtcaaag tatcgacacc gtactctttt tcatagtcag cagaaataac ttcgcgtggc
    44941 aatttacctg gcttacgaac aggcgcaaaa ccaattccca actcaaaggc aactggacaa
    45001 cccacgataa atccacgagc ttcaggtccc acgatcatgt cgactttctt gtcagtagca
    45061 tactgaacga tttcacgaac agcgtagcta taagcatttc catcagccat caaaggacta
    45121 atatcacgga aggtaatgcc ttcctttgga taattttcaa ttgttgcaat gtaatctttt
    45181 aaattcatct ttttctttct ttcaaagttt tttactctct attatagcat attttttaag
    45241 aaagaaaaaa ggaaaagtta acttcaataa ttatctaacg ttttgacgat ttataactag
    45301 ccatcgcaat aaagcccaat ttctgtttat tcttagcaaa cattttatac atagttaaaa
    45361 actgctttct attctccttt ttacaagcat ttacacaaat tttcaaagtt cctagcaaac
    45421 cttcgtcata aatcataccc gataatttca ttaatgtcat ttcaccagtc aatgctttca
    45481 catcacaata acctgattct atcatcacct gttcccaacc atcttgagtt aaaggaccta
    45541 catttacatg aattgcttgt gataattcct gtctgataga ctctttagct tccttaagaa
    45601 gcacatcatg tgtcaagaga agacctccag gttttaatac ccttagatat tccattacac
    45661 attttttctt agcttgatcg gcttgcatag tcagcatagc ttcatttata acaatatcaa
    45721 aactagcatc ttgataagga agtttcattg catttgctct ttcaaaactg attaaatgag
    45781 caacacctgc cgttccagca gattttttag ccacttctaa agcttgagca tccatatcaa
    45841 cagcagttat cttgcaacca aaacgctgtg ccaactcaat tgctgtagtt cccctattac
    45901 acgcaacctc tagtattctc ttttcttttg aaaatcctcc ttctgcaatt aaccaatctg
    45961 tggcacgctt tccacctgga cgtaagcgtt ttttccccaa ttttgctaaa aacttatgac
    46021 ctgcttctga catttgaaca cctctgtttt ttcttcatta taacacaaaa tgatattatc
    46081 tgacaattct aatctattat ttgataaact agaatcaaaa gcatacaagt caccataact
    46141 atagcataaa tttattacct ctctagttcg aattcaattg ctcacgtagt ttacgcatat
    46201 agcttcactt gtagatggta cccacaaata atactcaatg gaaatcaaag agcaaactag
    46261 gaaactagcc gtaagctgct caaagtacag ctttgaggtt gcagataaaa ctgacgaagt
    46321 cagtaatcat acgtacggca aggcgacgtt gacgtggttt gaagagattt tcgaagagta
    46381 ttaaagagca tcacaatata gatgcataat atgacttccc tagcaaaaag gagagaagca
    46441 ttttgtttct ctccctagat taactgatat ctctccacta caattgataa agagatatct
    46501 tttcttgtag tgctttgaat tttatttatt ttagtagatt gttttttcag tttctacatc
    46561 gaagaagtgt gctttgttca agtcaaatcc aagctcaact gttgcacctg tttgcaagta
    46621 gtcacgagca tcaacttttg caacaaactc gtctttacca acttggcagt aaaggtgaga
    46681 ttctgaacca agcagttctg atacagagat agtcgctttt acaacacagt ctgggaatgt
    46741 ttcaaggaaa gcaggttctg cattcacgtc ttctggacgg ataccaaaga tcaattcttt
    46801 tccttcgtag cctttttcac gaagaacttt caatgctcct tctggcactt tcaaacggaa
    46861 accgtcagaa acaatttcgc taccaaccaa tttcacgttg atgaagttca tagctgggct
    46921 tccgatgaat cctgcaacga atttgttaac tggatttttg taaacttctt gaggagtacc
    46981 gatttgttct acacgtccga tagtacctgt accagcaggg ttcttagtag ctgacataat
    47041 aacgatacgg tctgcaagtg tcatcgcttc tgtttggtcg tgagttacat agatagttgt
    47101 agctccgata cgacggtgaa ttttagcgat ttcagcacgc attgatacac gaagtttggc
    47161 atccaagttt gacaaaggtt cgtccatcaa gaatactttc gcatcacgga caatcgcacg
    47221 ccccatggca acacgttgac gttgaccacc tgaaaggtca gctggtttac gttccaagaa
    47281 ttctttcaat ccaagtattt cagctgcttc ttgaacacgt ttgttaatgt cttctttgct
    47341 gtatttacgc aatttcaaac cgaaagccat gttgtcataa acagtcatgt gtgggtaaag
    47401 agcgtagttt tggaatacca tggcgatatc acggtctttt ggagctacgt cgttgacaac
    47461 cacgccatcg atagatgcag taccttctgt aatgtcttca agaccagcaa tcatacggag
    47521 tgtagttgat ttaccacatc ctgaaggtcc tacgaaaacg ataaattctt tatctttgat
    47581 gttcaagttg aaatcttcaa ctgaatagtg ttcgctgttt ggatattttt tgtaaatatt
    47641 tttaagattc aattctacca tgaggtgaac tccttttgtc ttttgataat tatattatag
    47701 atgaaaacgc tttatatttc tatggcaaga tgaccaaaaa aataaaaaag ttctgtgcaa
    47761 cttgcacaaa actttagaga atatcggaga atttgaagaa gataatcttt attattcttc
    47821 tatgacaaaa gcagaaacaa ggatcagttt tcccatattt tcgctgattc tccactacat
    47881 ttgactggat tctaattttt tagagaaata caaaagagct agctttagct aactcttttc
    47941 ctatgcggag agagggactt gaaccctcac gacctaaagc ggtcacagga tccttagtcc
    48001 tgcgcgtctg ccaattccgc catccccgca tcgattactt tactagtata tcaacttttg
    48061 ggatgcttgt caacactttt tttcaaattt tttcattttc accaaccagg ttactcagaa
    48121 agttcattta gattttcatc tactaactta gctccgagtg tatttttgaa atgacctagg
    48181 gcaaattgat gattttcagg ccagatggaa gcaacagctg gtttaacaat ctcgatgtca
    48241 tatcctagat tataggcatc tatagctgta tgtaggacac agatatccgt caagacacct
    48301 gttaagataa cggtagacac tctacgctct ctcaaacgaa tatctaggtc agtccctgaa
    48361 aaagctgagt aatggcgttt atccatccaa aagacacgac tgtctgaacc atgctcttga
    48421 taaaagatcc ccaaatctcc atataaattc cgtccactcg tcccaatcag attatgagga
    48481 ggaaataact tactttccgg atggaaacaa tcgttttctt catgagcatc aatagtaaag
    48541 aagatataat ctcctcgttc aaaagctaat cgagttacct tgctgatggc atccgaaatc
    48601 gcctgagctg gagcacctgc tgtcagcttc ccactatcag caacaaaatc ttctgtataa
    48661 tcaatcgaaa ttaaagcctt tgtcattagt aatctctttt cttcacttct tcaaaaatat
    48721 ctgaaatcaa gaccttaaga taggttccct tcattccaag tgagcgactt tcaataatcc
    48781 ccgcagactc aagtttacga agagcattga caatcacaga gcgagtgatt ccgatacgat
    48841 ctgcaatcac tgacgcagtc aacttccctt catttccatt taattcccct aaaattgctg
    48901 aaacagcacg gagttcggag taagaaaggg tattgaccgc catggtgaca gcagtacgac
    48961 gacgaatatt tttctcatct tcttcacgtt ggaagttaag aagctgaatc ccaacaacgg
    49021 tactggcaat ctcaacaaga accaaggcct catcttcgaa ttttttatca ttacgccaaa
    49081 taatcaaaga accaaggcga atccccgata catgaatcgg tgcaatagtc gtcaagccat
    49141 ctggaaaatc atctctactc tcaacaggga aaatactcat atcatgctca acaggtaagt
    49201 ttgcttctgt ttcgtaaatc atattagccc cttgaacgta gtcatctggg aaaatcttag
    49261 tttggaagaa ttgctctacg cgatctgtat ttgttttata acgcataaaa tagccaagca
    49321 gacgtccctt actattgata atgcaggcat tgcaatgaat aatatccgct aactgacgcg
    49381 taatagcgtt gtaaggaagc tcatcctgca actgctcctc tgagcgtttc aaaattgatg
    49441 taatttttct agttttttct aataaatgtg ccatttttca cctcgaattt aatcgctatc
    49501 attataacat aaaaacgtct ctttttcaat aattatctga aaattcctta ttgacttgca
    49561 ttgacttaca a
//
LOCUS       DAVGJJ010000006        43603 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000006 DAVGJJ010000000
VERSION     DAVGJJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.006"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(32..1699)
                     /locus_tag="VVE35_000310"
     CDS             complement(32..1699)
                     /locus_tag="VVE35_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU3060566.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1702..2067)
                     /locus_tag="VVE35_000311"
     CDS             complement(1702..2067)
                     /locus_tag="VVE35_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3060567.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2224..2412)
                     /locus_tag="VVE35_000312"
     CDS             complement(2224..2412)
                     /locus_tag="VVE35_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU3060568.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2528..3013)
                     /locus_tag="VVE35_000313"
     CDS             complement(2528..3013)
                     /locus_tag="VVE35_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU3060569.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAVFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(3282..4826)
                     /locus_tag="VVE35_000314"
     CDS             complement(3282..4826)
                     /locus_tag="VVE35_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU3060570.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5039..5341
                     /gene="gatC"
                     /locus_tag="VVE35_000315"
     CDS             5039..5341
                     /gene="gatC"
                     /locus_tag="VVE35_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU3060571.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5341..6807
                     /gene="gatA"
                     /locus_tag="VVE35_000316"
     CDS             5341..6807
                     /gene="gatA"
                     /locus_tag="VVE35_000316"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU3060572.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQTTLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGDN"
     gene            6807..8249
                     /gene="gatB"
                     /locus_tag="VVE35_000317"
     CDS             6807..8249
                     /gene="gatB"
                     /locus_tag="VVE35_000317"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU3060573.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8378..8938
                     /gene="efp"
                     /locus_tag="VVE35_000318"
     CDS             8378..8938
                     /gene="efp"
                     /locus_tag="VVE35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU3060574.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            8960..9349
                     /locus_tag="VVE35_000319"
     CDS             8960..9349
                     /locus_tag="VVE35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3060575.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9342..9764
                     /gene="nusB"
                     /locus_tag="VVE35_000320"
     CDS             9342..9764
                     /gene="nusB"
                     /locus_tag="VVE35_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU3060576.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(9814..10041)
                     /locus_tag="VVE35_000321"
     CDS             complement(9814..10041)
                     /locus_tag="VVE35_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3060577.1"
                     /translation="MLSGLIFGISHLILSYRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(10246..10536)
                     /locus_tag="VVE35_000322"
     CDS             complement(10246..10536)
                     /locus_tag="VVE35_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060578.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNF"
     gene            complement(10554..10739)
                     /gene="briC"
                     /locus_tag="VVE35_000323"
     CDS             complement(10554..10739)
                     /gene="briC"
                     /locus_tag="VVE35_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU3060579.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(10898..11665)
                     /locus_tag="VVE35_000324"
     CDS             complement(10898..11665)
                     /locus_tag="VVE35_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU3060580.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(11662..12528)
                     /locus_tag="VVE35_000325"
     CDS             complement(11662..12528)
                     /locus_tag="VVE35_000325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU3060581.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(12565..13932)
                     /gene="accC"
                     /locus_tag="VVE35_000326"
     CDS             complement(12565..13932)
                     /gene="accC"
                     /locus_tag="VVE35_000326"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU3060582.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(13944..14366)
                     /gene="fabZ"
                     /locus_tag="VVE35_000327"
     CDS             complement(13944..14366)
                     /gene="fabZ"
                     /locus_tag="VVE35_000327"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU3060583.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRSTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(14363..14848)
                     /locus_tag="VVE35_000328"
     CDS             complement(14363..14848)
                     /locus_tag="VVE35_000328"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU3060584.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(14851..16086)
                     /gene="fabF"
                     /locus_tag="VVE35_000329"
     CDS             complement(14851..16086)
                     /gene="fabF"
                     /locus_tag="VVE35_000329"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU3060585.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISTEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(16108..16839)
                     /gene="fabG"
                     /locus_tag="VVE35_000330"
     CDS             complement(16108..16839)
                     /gene="fabG"
                     /locus_tag="VVE35_000330"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU3060586.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(16873..17793)
                     /gene="fabD"
                     /locus_tag="VVE35_000331"
     CDS             complement(16873..17793)
                     /gene="fabD"
                     /locus_tag="VVE35_000331"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU3060587.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(17786..18760)
                     /gene="fabK"
                     /locus_tag="VVE35_000332"
     CDS             complement(17786..18760)
                     /gene="fabK"
                     /locus_tag="VVE35_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU3060588.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(18879..19103)
                     /locus_tag="VVE35_000333"
     CDS             complement(18879..19103)
                     /locus_tag="VVE35_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU3060589.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(19163..20137)
                     /locus_tag="VVE35_000334"
     CDS             complement(19163..20137)
                     /locus_tag="VVE35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU3060590.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(20137..20571)
                     /locus_tag="VVE35_000335"
     CDS             complement(20137..20571)
                     /locus_tag="VVE35_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3060591.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(20665..21450)
                     /locus_tag="VVE35_000336"
     CDS             complement(20665..21450)
                     /locus_tag="VVE35_000336"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU3060592.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            21775..23139
                     /locus_tag="VVE35_000337"
     CDS             21775..23139
                     /locus_tag="VVE35_000337"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU3060593.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTNEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDVTGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            23142..23513
                     /locus_tag="VVE35_000338"
     CDS             23142..23513
                     /locus_tag="VVE35_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU3060594.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            23727..25001
                     /gene="serS"
                     /locus_tag="VVE35_000339"
     CDS             23727..25001
                     /gene="serS"
                     /locus_tag="VVE35_000339"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU3060595.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEIKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(25026..25925)
                     /locus_tag="VVE35_000340"
     CDS             complement(25026..25925)
                     /locus_tag="VVE35_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU3060596.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPNWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(26035..26583)
                     /locus_tag="VVE35_000341"
     CDS             complement(26035..26583)
                     /locus_tag="VVE35_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU3060597.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(26801..28123)
                     /locus_tag="VVE35_000342"
     CDS             complement(26801..28123)
                     /locus_tag="VVE35_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU3060598.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFTALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSQVVIAETKKYFDK"
     regulatory      complement(28214..28302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(28466..30802)
                     /locus_tag="VVE35_000343"
     CDS             complement(28466..30802)
                     /locus_tag="VVE35_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU3060599.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(30906..31454)
                     /locus_tag="VVE35_000344"
     CDS             complement(30906..31454)
                     /locus_tag="VVE35_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU3060600.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(31451..31753)
                     /gene="zapA"
                     /locus_tag="VVE35_000345"
     CDS             complement(31451..31753)
                     /gene="zapA"
                     /locus_tag="VVE35_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU3060601.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            31840..32721
                     /locus_tag="VVE35_000346"
     CDS             31840..32721
                     /locus_tag="VVE35_000346"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU3060602.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGNRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            32725..33339
                     /gene="lepB"
                     /locus_tag="VVE35_000347"
     CDS             32725..33339
                     /gene="lepB"
                     /locus_tag="VVE35_000347"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU3060603.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            33419..33541
                     /locus_tag="VVE35_000348"
     CDS             33419..33541
                     /locus_tag="VVE35_000348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEU3060604.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            33618..35984
                     /locus_tag="VVE35_000349"
     CDS             33618..35984
                     /locus_tag="VVE35_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU3060605.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDS
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAVTRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(36032..37315)
                     /locus_tag="VVE35_000350"
     CDS             complement(36032..37315)
                     /locus_tag="VVE35_000350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU3060606.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(38101..39237)
                     /locus_tag="VVE35_000351"
     CDS             complement(38101..39237)
                     /locus_tag="VVE35_000351"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU3060607.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(39299..39736)
                     /locus_tag="VVE35_000352"
     CDS             complement(39299..39736)
                     /locus_tag="VVE35_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3060608.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(39738..41693)
                     /locus_tag="VVE35_000353"
     CDS             complement(39738..41693)
                     /locus_tag="VVE35_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU3060609.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVRELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(41717..43486)
                     /locus_tag="VVE35_000354"
     CDS             complement(41717..43486)
                     /locus_tag="VVE35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU3060610.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGSIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        12849 a         9871 c         8272 g        12611 t
ORIGIN      
        1 aaagaactga tttctcagtc cttcattaat cttattccac actaaatagg tatgggtaaa
       61 caggttgttg accttggtga atctcgactt caacgtcttc gaattcttct acgatttctt
      121 gagcgatttc attggcaagt tcttcgcttc cgtcttcacc gacatagaag gttacgattt
      181 cactgtcttc atccaacata tgtttcaagg tttcagtcaa tgtttggtgc atatcagggt
      241 ttgacacaag aatttttcca tccaccatac ctagattatc gttttcatgg atttctaagc
      301 catcgatcgt tgtatcacgc acggctgttg tgacgcttcc gctaacgaca tctctaagag
      361 cagctgtcat acgctcttgg ttttcttcaa tggacttgct tggatcaaag gcaagaagac
      421 ttgtcatacc ttgaggaaga gtgcgagcct ctaccactac tgctggttgc tccaaaactt
      481 ctgccgcaga ttgagctgcc atgaagatgt tcttgttgtt tggcaagaag atgatgttac
      541 gggcattgac ctgttcaaca gccttgataa agtcttctgt tgaagggttc atggtttgac
      601 cgccttcgat aacataatcc acgccttgag aacagaagat atctgctaga cctttaccag
      661 ccaccacagc aatcaaagca tactcttttt cttcagccga cttgataact tgagtagctt
      721 ctttctcaac ctgtgcttcg tgttggttac gcatattgtc aacttttacc ttgaccaagc
      781 taccatattt gagaccttct tgcataacaa gtcctggatc ttctgtatga acatggactt
      841 tgacaatttc atcatcgtta acaacgagga gagaatcccc aagctcatcc aagtagttac
      901 ggaattcatc gtagtcaaaa tctttagcat aggttggacc ttgcttaaga gctaccatga
      961 tttcagtaca gtaaccaaac gtgatgtcct cagttgctac gtgaccagct acagacttat
     1021 gatgctctac attgatcatc tcactcatgt tggcaggagt cgctacaaag tcctcagatg
     1081 caatatattc gccagtaagg gctgaaagga aaccttcgta gatgaagacc aatccttgac
     1141 cacctgagtc cacaacgcca acttctttca atactggaag catgtctggt gttttagcta
     1201 gagctgtttt agcaccttcc aaggctgcgc gcatgacttc aacagtgtca tctgtttgct
     1261 cagctttttt cttagcaccg atagcagctc cacgagaaac tgtcaaaatc gttccttcaa
     1321 caggtttcat cactgcctta taggcaactt ccacacctga ttggaaggcc agagccaagt
     1381 cttgacctgt taactcgtct ttatccttga tagcttggga aaatccacgg aaaagctgag
     1441 acgtaatcac tcctgagttc ccacgcgcac ccatcaaaag ccctttggca agaatgctcg
     1501 ctacttctcc aactgtagaa gctggcttgt ctgcaacttc tttagcacca ttttcaatgg
     1561 tcattcccat atttgtccca gtatctccat ctggaactgg aaagacgttt aatgaattga
     1621 catattcagc ttgcttattc aagcgagttg atgcagcctg caccatttct tgaaataagc
     1681 tagtagtaat ttttgacacg gttattctcc tacaactttg atattttgaa tgtagacatt
     1741 tacagtctga gcagtaattc caagctggtt ttccaagcta aaggcaacac gctcttgaat
     1801 gttttttgac acttcactaa tctttgttcc gtagcttaac acggtatata catcaactgc
     1861 aatactgcca tcttcggctg cctttacgac gacaccttta gaataatttt ccttacctag
     1921 cagggcttgg aaattatctt tgagggcatt tttactagcc ataccgacca caccaaaaat
     1981 ctcagttgct gctccaccta cgacggttgc aatcacttca tctgttagtt cgatttgacc
     2041 atcttttgta ttaattttta cagtcatcct ttttacctca actagttgat actctatttt
     2101 atcatatttc agcccaagtg taaaagcaga atgatgtatc agcggatatt tactctattt
     2161 ttcaaacgat gttataccca caataaaaga aaaaagaccc taaggtctcc tttgctttta
     2221 ttattaaacg cgttcaactt tacctgattt caaagcacga gctgaagccc aaactttttt
     2281 aggtttacca tcgataagaa cagtaacttt ttgaaggttt ggttttacgg cacgttttgt
     2341 ttggttcatc gcgtgtgaac ggttgtttcc tgatacagtc ttacgacctg taaagtaaca
     2401 tactttagcc attgtgtttt cctcctatta gatctaatat agcggatgtg ctagcaccac
     2461 ataccgtact atgttatcac actttcttgt tttttgcaag ggaattggaa gattttttat
     2521 ttgtgtctta aatcaggtct tgcgtgacat tttctgctct ccacatgcca ttgttgatta
     2581 acagaacacc agaattaaaa ttatgtgtat aaaaatcatc tctaactgca gctaagggta
     2641 tagccgtcaa gtccaaatcc cacagctcat ctatcgattt tcttacaaca atatctgaat
     2701 ccaaatacag tacacgagac tcgcttacat actttggaat aaaataccta aaaaaaaccg
     2761 catatgaaag tccctcaaag gggagacgat aacctttcag aatactactg tcaatctgaa
     2821 cattcacaat ctcactattc aaagtctcta gtcttttttc catcaattgg aaccattctc
     2881 gcggaaggtc atcattaaaa acataaaact taagattata atgatgaaca caaagagatt
     2941 ttattgttgt ttcaacttta tccatataag cattatctgc acctaagaca atcgcttttt
     3001 tctcttcttt cactctttat cttatttctt tttattccca tcatattatt cccatcatat
     3061 gtttctacgt aaccattatt ttcgcctatt cgttcgtaaa accataccag tggagatttt
     3121 agatgaagtc ccattacggt ttacaatttt tacattacga cacggagttt tacaaatcga
     3181 tttcatttgc caaacgtagt tagtgaggca gttagctagt tcgccaaata gcgactagcg
     3241 tccaacaatt tggaacttta gttccaattg ttggtactga gtcacatctt ctcctctaac
     3301 tctacgtctg gatacttgtc cgcaaaccag cggagggcaa agtcattttc aaagagaaag
     3361 actggttggt caaaacggtc tttggctaag atattgcgac ttgacgacat ccgttcatcc
     3421 aagtcctcag gcttgatcca acgaacggtc tttttaccca ttgggttcat aactacttcc
     3481 gcattgtact cgccttccat gcggtgttta aagacttcaa actggagttg acctacagcg
     3541 cctagcatgt actcacctgt ttggtaattc ttataaagct gaacggctcc ttcttgcacc
     3601 aattgctcaa tccccttgtg gaaggatttt tgcttcataa cattcttagc agaaactttc
     3661 atgaaaatct caggtgtaaa ggttggcagg ggttcaaatt caaacttgtt ttttccaacc
     3721 gtcaaggtat ccccaacctg ataagtacca gtatcgtaaa ccccgataat atcacctgcc
     3781 acggcattgg tcacattctc acgactctcc gccataaact gggtaacatt agatagtttg
     3841 gcacccttac cagtacgagg gagattgaca ctcatgccac gctcaaattc gcctgatacg
     3901 atacggacaa aggcaatacg gtcacggtga cgaggatcca tgttggcttg gattttaaag
     3961 acaaagcctg agaaatcctt gtcataagga tccacaattt caccgtctgt tttcttgtga
     4021 ccatgtggtt ctggagcaaa cttgaggaag gtctcaagga aggtctgcac accaaagttt
     4081 gtcagggctg aaccgaaaaa gacaggcgtc aattctccag ccagaatagc ttcctctgaa
     4141 aactcattcc cagcttcatt taaaagctca atgtcatcct tgacttgctc gtagaaagga
     4201 ttgctaccaa aaagtttgtc cccatcttct aggctagcaa aacgctcatc ccctttgtaa
     4261 agctctaaac gttggttata gaggtcatac aagccctcaa aggctttccc catcccgata
     4321 ggccagttca tagggtagct agcaatgccc aagatttctt ccaattcttg caagagatcc
     4381 aaaggctcac gaccgtcacg atccaacttg ttcataaagg taaagactgg aatgccacga
     4441 tgtttcacaa cctcaaacaa tttcttggtt tgagcctcga tccccttggc agagtccacg
     4501 accatgaccg cagcatccac cgccatcaag gtacgatagg tatcttctga gaagtcctcg
     4561 tgccctggcg tgtctaagat attcacgcgc ttgccgtcgt agtcaaattg cataacagat
     4621 gaagtaacag aaatcccacg ttgcttctcg atatccatcc agtcagattt agcaaaagtc
     4681 cctgttttct tcccttttac cgtaccagcc tcacgaatct cacccccaaa gtagagtaac
     4741 tgctcagtga tggttgtttt ccccgcgtcc gggtgggaga taatggcaaa ggtacgacgt
     4801 ttcttaattt cttcttgaat attcataagt tctctttctt tgattctcta tttttcttgt
     4861 ttcaatagct gagaatgatt tttacattgg attttaccat tcctttcaac actccattat
     4921 atcggatttt agcatttttt tcaatttcta tttcttttca cttccccctc ccttatttat
     4981 aggaaaatat ggtaaaatag aacagactaa aaatcatcat ttcacgaaag gatgcaagat
     5041 gaaaattacg caagaagagg taacacacgt tgccaatctt tcaaaattaa gattctctga
     5101 aaaagaaact gctgcctttg cgaccacctt gtctaagatt gttgacatgg ttgaattgct
     5161 gggcgaagtt gacacaactg gtgtcgcacc tactacgact atggctgacc gcaagactgt
     5221 actccgccct gatgtggccg aagaaggaac agaccgtgat cgcttgttta aaaacgtacc
     5281 tgaaaaagac aactactata tcaaggtgcc agctatccta gacgatggag gagatgccta
     5341 atgactttta acaataaaac tattgaagag ttgcacaatc tccttgtctc taaggaaatt
     5401 tctgcaacag aattgaccca aacaacactt gaaaatatca agtctcgtga ggaagctctc
     5461 aattcatttg tcaccatcgc tgaggagcaa gctcttgttc aagctaaagc cattgatgaa
     5521 gctggaattg atgctgacaa tgtcctttca ggaattccac ttgctgttaa ggataacatc
     5581 tctacagacg gtattctcac aactgctgcc tcaaaaatgc tctacaacta tgagccaatc
     5641 tttgatgcga cagctgttgc caatgcaaaa accaagggca tgattgtcgt tggaaagacc
     5701 aacatggacg aatttgctat gggtggttca ggtgaaactt cacactacgg agcaactaaa
     5761 aacgcttggg accacagcaa ggttcctggt gggtcatcaa gtggttctgc cgcagctgta
     5821 gcctcaggac aagttcgctt gtcacttggt tctgatactg gtggttccat ccgccaacct
     5881 gctgccttca acggaatcgt tggtctcaaa ccaacctacg gaacagtttc acgtttcggt
     5941 ctcattgcct ttggcagctc attagaccag attggacctt ttgctcctac tgttaaggaa
     6001 aatgccctct tgctcaacgc tattgccagc gaagatgcta aagactctac ttctgctcct
     6061 gtccgcatcg ccgactttac ttcaaaaatc ggccaagaca tcaagggtat gaaaatcgct
     6121 ttgcctaagg aatacctcgg tgaaggaatt aacccagagg ttaaggaaac aatcttaaac
     6181 gcggccaaac actttgaaaa attgggtgct attgtcgaag aagtcagcct tcctcactct
     6241 aaatacggag ttgccgtata ctacatcatc gcttcatcag aagcttcatc aaacttgcaa
     6301 cgctttgacg gtatccgtta cggctatcgc gcagaagatg caaccaacct tgatgaaatc
     6361 tatgtaaaca gccgaagcca aggctttggt gaagaggtaa aacgtcgtat catgctgggt
     6421 actttcagtc tttcatcagg ttactatgat gcctactaca aaaaggctgg tcaagtccgt
     6481 accctcatca ttcaagattt cgaaaaagtc ttcgcggatt acgatttgat tttgggtcca
     6541 actgctccaa gtgttgccta tgacttggac tcactcaacc atgacccagt tgccatgtac
     6601 ttagccgacc tattgaccat acctgtaaac ttggcaggac tgcctggaat ttcgattcct
     6661 gctggattct ctcaaggtct acctgtcgga ctccaattga ttggtcccaa gtactctgag
     6721 gaaaccattt accaagttgc tgctgctttt gaagcaacaa cagactacca caaacaacaa
     6781 cccgtgattt ttggaggtga caactaatga actttgaaac agtcatcgga cttgaagtcc
     6841 acgtagagct caacaccaat tcaaaaatct tctcacctac ttctgcccac ttcggaaatg
     6901 atcaaaatgc caacactaac gtgattgact ggtctttccc aggagttctg ccagttctca
     6961 ataaaggggt tgtcgatgcc ggaatcaagg ctgctcttgc cctcaacatg gacatccaca
     7021 aaaagatgca ctttgaccgc aagaactact tctatcctga taaccccaaa gcctaccaaa
     7081 tttctcaatt tgatgaacca atcggatata atggctggat tgaagtcgaa ctagaagacg
     7141 gtacgaccaa gaaaatcggt atcgaacgtg cccacctaga ggaagacgct ggtaaaaaca
     7201 cccatggtac agatggctac tcttatgttg acctcaaccg ccaaggggtt cccttgattg
     7261 agattgtatc tgaagcagat atgcgttcgc cagaggaggc ctatgcttat ctgacagcac
     7321 tcaaggaagt tatccagtac gctggcattt ctgacgttaa gatggaggaa ggttcgatgc
     7381 gtgtggatgc aaacatctcc cttcgtcctt atggtcaaga gaaattcggt accaagactg
     7441 aattgaagaa cctcaactcc ttctcaaacg ttcgtaaagg tcttgaatac gaagtccaac
     7501 gccaggctga aattcttcgc tcaggtggtc aaatccgcca agaaacacgc cgttacgatg
     7561 aagcgaataa agcaaccatc ctcatgcgtg tcaaggaagg ggctgctgac taccgctact
     7621 tcccagaacc agacctaccc ctctttgaaa tttctgacga gtggattgag gaaatgcgga
     7681 ctgagttgcc agagtttcca aaagaacgtc gtgcgcgtta tgtatctgac cttggtttat
     7741 cagactacga tgctagtcag ttgactgcta ataaagtcac ttctgacttc tttgaaaaag
     7801 ctgttgccct aggtggtgat gccaaacaag tctctaactg gctccaaggg gaagtcgctc
     7861 agttcttgaa tgctgaaggt aaaacactgg aacaaatcga attgacacca gaaaacttgg
     7921 ttgaaatgat tgccatcatc gaagacggta ctatttcatc taagattgcc aagaaagtct
     7981 ttgtccatct agctaaaaat ggcggtggcg cgcgtgaata cgtggaaaaa gcaggtatgg
     8041 ttcaaatttc agatccagct gttttgattc caatcatcca ccaagtcttt gccgataacg
     8101 aagctgctgt tgccgacttc aagtcaggca aacgtaacgc cgacaaggcc tttacaggat
     8161 tccttatgaa ggcaaccaaa ggccaagcca acccacaagt tgcccttaaa ctacttgcac
     8221 aggaattggc gaagttgaaa gaaaactaga cagaacaaaa ccagccctaa ggttggtttt
     8281 ttcttctcta ccaactccca ataactattt tggctttatt tccagagtat tttatggtaa
     8341 aatgaagagt aataatattt attaaagagg taaaaacatg attgaagcaa gtaaattaaa
     8401 agctggtatg acctttgaaa cagctgacgg caaattgatt cgcgttttgg aagctagtca
     8461 ccacaaacca ggtaaaggaa acacgatcat gcgtatgaaa ttacgtgatg tccgtactgg
     8521 ttctacattt gacacaagct accgtccaga ggaaaaattt gaacaagcta ttatcgagac
     8581 tgtcccagct caatacttgt acaaaatgga tgacacagca tacttcatga atacagaaac
     8641 ttacgaccag tacgaaatcc ctgtagtcaa tgttgaaaac gaattgcttt acatccttga
     8701 aaactctgat gtgaaaatcc aattctacgg aactgaagtg atcggtgtca ccgttcctac
     8761 tactgttgag ttgacagttg ctgaaactca accatctatc aaaggtgcta ctgttacagg
     8821 ttctggcaaa ccagcaacga tggaaactgg acttgtcgta aacgttccag acttcatcga
     8881 agcaggacaa aaactcgtta tcaacactgc agaaggaact tacgtttctc gtgcctaatc
     8941 tctagaaaga ggtcattcta tgggaattga agaacaactt ggcgaaatcg ttatcgcccc
     9001 acgtgtactt gaaaaaatca ttgctattgc tactgcaaag gtagagggtg ttcactcttt
     9061 ttcaaacaga tcagtgtctg ataccctttc aaaactttca ctcggccgtg gcatttatct
     9121 taaaaacgtg gacgaagaac tcacagcaga tatctatctc taccttgagt acggagtaaa
     9181 agttcctaag gtagcggttg ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc
     9241 tgatgtagaa ctcgctgcta tcaatattca cgttgcaggt atcgtcccag ataaaacacc
     9301 aaaaccagaa ttgaaagatc tatttgacga ggacttcctc aatgactagt ccactattag
     9361 aatctagacg ccaactccgt aaatgcgctt ttcaagctct catgagcctt gagttcggta
     9421 cggatgtcga aactgcttgt cgtttcgcct atactcatga tcgtgaagat acggatgtac
     9481 aacttccagc ctttttgata gacctcgttt ctggtgttca agctaaaaag gaagaactag
     9541 ataagcaaat cactcagcat ttaaaagcag gttggaccat tgaacgctta acgctcgtgg
     9601 agagaaacct ccttcgcttg ggagtctttg aaatcacttc atttgacact cctcagctgg
     9661 ttgctgttaa tgaagctatc gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt
     9721 ttatcaatgg actgctcagc cagtttgtaa cagaagaaca ataaaaagaa aaagtgtttg
     9781 atagatatca aacacttttt tctttgcctc ccactatcta aaaaaatgat aatagatata
     9841 attgtaaaca aaaatccaga taggttttgc atgattgaga aagttaaaaa aactatggca
     9901 gagaatcgtt aatctcagat tgtcggtaga acgataaaca agggcaaaaa agaaaccaat
     9961 cagactataa tataataaac taattggatc tctgtaagat agtatcaaat ggctaatccc
    10021 aaagataagg cctgataaga tgacatctaa atagtacttg gactagggaa agaaggtatt
    10081 cataaaatac cctctatcaa gagtctcctc aaaaacagga ccgattatta caagcaggac
    10141 aaaagataag atagtcgata aaaaggttgg ttgtccattt gaaagaacca cggtaaaata
    10201 ctcatcatga atattcctat ggttaatcaa atgagcatag cgtgcctaaa aattaccgag
    10261 aatctgataa accacataag ttgcaaataa gtagaagaca aatgaccagt tccagctctt
    10321 tttttcaaag ataaagagca tctttttctt ttttaacctc caaattaata gaaggaaact
    10381 tcccactaat accattgtta aaataagaga atagacatca gctcctaacc ctaaaatgat
    10441 cgtcacatac aatccaattg tttgtggtaa ataggtagat agtaaaataa taagcaaaaa
    10501 tattccaaat tgtcttagtt tttttgtgtt tctcatcgta cttttttgaa agattaccct
    10561 gctcggaagc cgtacttcca agcatctata taagaattaa gtgccccttg cctcatatag
    10621 ggagcaaatt ctctataata taaccatcta ctatatccat cttcccaaac agcaagacca
    10681 cctgaagttt gctccaagtc ctcagttgaa agaactgtaa atgtatttgt acctgtcatt
    10741 gcaagtacct tcttaaaata gattgttgta gactcacatt tatagtatat ttcttttttt
    10801 gtctatttta tagcccatct cctcaactgt caatttttca acctgaattg catttttcca
    10861 taaaaaatga gacctttcta gtctcattta gtcattctta gtattttcta aatcgttgat
    10921 agcgttcttc cagcaactct tctagcggtt tttgtgaaag tctagccagc tccgtttgga
    10981 gttctttttt gacactctta atcagttctt tactagaaag tcctatttca gaaatcacct
    11041 tatccaccac atccatttct aacagttcat gcgaagtgat tttcatcagt tctgctgctt
    11101 ccatggcgcg agtaccgtcc ttccataaaa tggaagcaaa gccttctgga ctgagaatgg
    11161 catagataga attttccagc atccagacac ggtccgcgac agctagagcc agagccccgc
    11221 ctgaaccacc ttcaccgata ataatggcga taataggaac tttcaggtca ctcatttcca
    11281 tgagattgcg agcaatagct tccccttgac cacgttcttc cgctccgaca ccaggataag
    11341 cacctgctgt attgataaag gtcacaactg gacggccaaa tttctcagcc tgtttcatca
    11401 accgcagtgc ctttcggtag ccttctggat gtggttggcc aaaatttcgt ttgaggttgt
    11461 cttgtaaact cttgcctttt tggataccaa ccactgttac agcttggtct ccaagccaac
    11521 caataccacc aacaactgca ccatcatcac gaaaagaacg gtcaccatgt aattggataa
    11581 attcatcaaa aatgcctgtc gcaaagtcca aggttgtcaa gcgactctgc tcacgcgctt
    11641 ctctgactat ttttgcaata ttcatctagg actccctcca tgcaatctga ctaggctagc
    11701 aatcgtatct ggtaagtctc ttcttttgac aatagcatcc acaaagccat gttctaatag
    11761 gaattctgcc ttttggaaat cttcaggcaa gctttcacga accgtatttt caatcacacg
    11821 acgcccagca aaaccaacca agctctgtgg ttcagccaga atgatatcgc cttccatagc
    11881 gaaagaagct gtcacaccac cagtcgttgg atctgtcaaa atggtcaggt aaaagagacc
    11941 agcatttgaa tggcgtttaa ccgccgcaga gatcttagcc atctgcatga gactcatgat
    12001 tccttcctgc atacgggctc caccagaggc tgtgaatagg acaactggca atttttcgac
    12061 agtcgcatac tcaaacaaac gagtgatttt ttcacctaca accgtaccca tagaagccat
    12121 gataaagtta gaatccataa tcccaagagc cacagtctga cctttaataa gagcagttcc
    12181 tgtcacaacg gcttcatgca gacctgtttt ttcacgcata gatgccagtt tcttttggta
    12241 accagggaaa tgcaagggat ccttgctttc aatccctgta aacaattctt tgaaggttcc
    12301 catatcaatc gtcaaagcca agcgttcttg ggcagaaata cgaaaggtat agctacagtg
    12361 cggacagata cgttcacttc ccagatcctt ctgatagatg gtatgcttac agcctggaca
    12421 ctgggaaaat aattcatctg gaacctctgg cttagcttga ggtttttccc taaccgaacg
    12481 attgggattg attcgaatat acttatcttt tttactaaat agagccattg attccccttt
    12541 tcggtttaaa ctcttaaagt cattttattc tttttcttga tatttaggta agaaggtttc
    12601 catcaagaag gaagtatcat aatccccagc aatgacattg cgatctgaaa tgaggtcaag
    12661 ctggaaatct gcattggtct gcactccttc aatctctaat tcatagaggg cacgttgcat
    12721 tttcatcaag gcgtcaaaac gattttcgcc gtgtactatg attttggcaa tcatactatc
    12781 ataataaggc ggaatggtat aacctggata aactgctgaa tccacgcgca agccaactcc
    12841 accacttggc agatagagat tagtaatctt acctggactt ggagcaaagt taaaggctgg
    12901 gttttctgca ttgatacgac actcgatggc atgaccgcgt aggacaatat cttcttgctt
    12961 aacagacaaa ggctgacctg ccgcaatgca aatctgttcc ttaacgatat caacacctga
    13021 aacaaactct gttactggat gttctacctg aacacgagta ttcatctcca tgaaatagaa
    13081 attgctactt gcttcatcaa gaagaaattc aatggttcct gcattctcat agccaacaaa
    13141 ctctgccgct cgaacagcag cagcacctat ttcatgacgc agcgtttttc cgattgcaat
    13201 cgagggactt tcttccaaaa ccttttggtt attcctttga agagaacaat cccgctcacc
    13261 caagtgaatc acatgtccat gctcatcacc taggatttga acctcaatgt gccgagctgg
    13321 atagataacc cgttctatgt acatggcacc attgccataa ttggccttgg cctcactaga
    13381 ggcagtttca aaggcagaaa cgaggtcatc tggtttttca accttacgaa tccctttacc
    13441 acctccacct gctgaagcct tgagcataac aggatagcca attttttcag caacaatcaa
    13501 agcttcttca gagttatgca cttctccatc tgaacctggt ataacaggca cacctgcttt
    13561 aatcatctga gcacgcgcat tgatcttatc ccccatcata tccataacat gaccagatgg
    13621 accgataaac ttgataccta cttcttcaca catggtcgca aatttggaat tttcactgag
    13681 aaatccaaaa ccagggtgaa tagcttctgc ctcagtcaag actgcagctg atagaactgc
    13741 attaatattg agataagact ctgttgcctt gccaggacca atacaaactg cttcatctgc
    13801 caaaagcgta tgaagagctt ccttatcagc agttgaataa accgctaccg tcgcaatccc
    13861 caattcacgt gccgcacgga taatacgaac cgcaatttca ccacgattgg caattaaaat
    13921 ttttcgaaac atggagaacc tccttagttc ccaattgcaa aagtaaggat accactggct
    13981 gcaagcttgc catccacttc agcctttgct tcaaccacag ctatggtgct acgacgtttt
    14041 acaaaagtcg ctgtcataac caattggtcg cctggtacaa cttgcttctt gaacttaacc
    14101 ttgtccatac cagcgtaaaa gaccagtttt cctttatttt caggttttga taactccaac
    14161 acaccagcag tttgcgccaa ggcttccata atcagaacac ctggcataac tgggtattga
    14221 ggaaagtggc cgttaaagaa aggctcgtta atggtcacat ttttgatagc aacaatggta
    14281 tcctcgctca cttccaagac acggtccact agaagcatag gataacggtg gggaagggct
    14341 tctttgattc cttgaatatc gatcatttga tacgtaccaa tcctttacca aactcaacca
    14401 tttcctcgtt agagacgaga atttccgtta ccacaccatc cttaggagct gggatttcat
    14461 tcatgacttt catggcttcg ataattacca atgtttgacc ttttttgaca ctatcaccaa
    14521 ctgtaacgaa ggcaggttta tctggtccag cagccaagta aaccactcca acaagtggac
    14581 tctctacaag atttccctca gtagccacac ttgcttcagc tggagctgga acttcttctg
    14641 ctacagtctc tgctggagca gatgtaggag ctactggact cggtgttgct agaacgggtg
    14701 ctggagcgac ttgagttgca acttcaggca caggtctcgc ttcattcttg ctaaactgca
    14761 actcatccgt cccattttta taagaaaatt ctctcaaact tgactggtca aattgagtca
    14821 tcaagtcttt aatatcattt aaattcatac ttatctattc tcccaacgtt tgaaagcaag
    14881 aactgcattg tggcctccaa aaccaaaagt atttgaaata gcgtatggaa tttctttctc
    14941 caagccttgt ccataaacga cattagcttc gatataatct gatacttcac ttgtcccagc
    15001 tgtcattggt acaaagttat gacgcatagc ttcgatggtg acgatagctt ctactgcacc
    15061 cgcagccccc agcaaatgtc ctgtaaaaga cttggttgat gatacaggta cttccttacc
    15121 aagaacagct acgatagctc cactttctcc tttttcattg gcaggagttg acgttccgtg
    15181 agcattgaca taggctactt gctctgtaga aatctcagct tcttccaagg ctagtttgat
    15241 ggccttgata gctccctgac cttctggatg tggagaagtc atgtggtagg catcacaagt
    15301 atttccgtaa ccaaccactt cagccaggat agtagctcca cgtttttcag cgtgttcaag
    15361 gctttctaga accaacatcc ctgaaccttc acccataaca aacccattgc gatccttatc
    15421 aaatgggatc gaagcacgag ttggatcctc tgtagtagag agagctgtta aggcttggaa
    15481 accagcgatg gcaaaaggtg tgatagaagc ttctgttcct cccaccaaca tcacatcttg
    15541 gaaaccaaac ttaatggagc ggaaggcatc cccaatcgca tcatttgatg aagagcaggc
    15601 agtattgata gatttacaaa caccgtttgc accaaaacgc atggctacat tcccagaagc
    15661 catatttggt aaagcttttg gaagagtcat tggtttgaca cgtttgggtc ctttttcatg
    15721 aaggcgaagt acctgatctt caatttcctt gattccacca ataccagatg caacgataac
    15781 accaaaacga tccctattaa gagcctctac atcaagattg gcatgattta cagcctcttg
    15841 ggctgcatac aaggcatata aagaatagtt atcaaaacgg ttggtatctt tttttacaaa
    15901 gtatttatcg aacggaaaat cttggatttc tgccgcatta tgcacatcaa agtcactatg
    15961 atcaaatttt gtaatgccac caatgccgat tttcccagtt gctaaactat tccaaaattc
    16021 ttctggtgta tttccgattg gagatgttac tccataacct gttaccacta ctcgatttag
    16081 tttcattctt ttcacctcta gctttcgcta catacttaag ccaccatcaa tggcaaccac
    16141 ttgaccagtt agataatctt ggcctgctaa aaatactgtc aaatctgcaa cctgctctgc
    16201 ctgcccaaat tctttcatcg gaatctgagc tagtgtagct tccttaatct tatctgataa
    16261 gatagctgtc atatcagact caatcattcc tggagcaatc acattgactc gtatattccg
    16321 actagcgacc tcgcgtgcca cagacttggt aaagccaatc aagccagcct tagaagcagc
    16381 atagttagct tgaccaatat tccccatcaa accaacaaca ctagacatat taatgatagc
    16441 accttctctg gctttcatca tcggtttcaa gactgattgt gtcatattaa aggcaccagt
    16501 cagattgacc ttgagcactt tttcaaaatc tgcttctgtc atcttgagca taagagtatc
    16561 ttgggtaatc cctgcattgt tgaccaaaac atctactgaa cccagttctg caatagcttg
    16621 atcaatcata cgcttagcgt ctgcaaaatc tgatacatct cctgaaatgg gaaccacctt
    16681 gataccatag tttgaaaact cagcgagcaa ttcttctgag attgccccac gactgtttaa
    16741 gacaatgttg gctcctgctt gagcaaactt gtgggcgatg gcaagaccaa ttccacgact
    16801 cgaacctgta ataaagatat ttttatgttc tagtttcatt tttttccttt caaaacttct
    16861 acttatttta gtctattttt ctaaaagtgc tactaaactc gcttgatctt ccacatgagc
    16921 taagtgagca gtttgatcaa tttttttaac aaaacctgac aagactttcc ccggtccaat
    16981 ctcgataaag ttgcttatgc ctgcttcttg catgacccca atactttcat agaaacgaac
    17041 gggttccttg acctgacgcg tcaagagctg agcaatgtcc tctttttgca tcacagcagc
    17101 ttctgtattg ccgactaggg gacaagtaaa atctgaaaaa cttacctgag ctagagtttc
    17161 agctagtttc tggctagcag gctcaaggag agcggtgtga aagggacctg acaccttaag
    17221 aggaatcaag cgtttggcac ctgcttcttg caaaagttca accgctcgat caactgcaac
    17281 cacttctcca gcaatgacga tttgtgcagg tgtgttatag ttggctggag taaccactcc
    17341 aagttcagaa gctttttgac aggcttcttc aatgacctct actggcgtat tgagaactgc
    17401 taccatcttg ccagagtcag caggagccgc ttcttccata taggctccac gcttagctac
    17461 caaggcaacc gcatcttcaa aatccaaggc gccgcttgcc accaaggcag agtattctcc
    17521 aagagacaaa ccagcaacca tatcaggctg atagcccttt tcttgcaata aacggtagat
    17581 agcaaccgaa gtcgctagaa tggctggttg cgtatagcgg gtctgattga gtttgtcttc
    17641 ttccgtatcg atgagataac gtaaatcata accgagcacc tgactcgctc gatcaatcgt
    17701 ttctttgaca atcggatact gatcatagaa atcccgtccc atccctagat actgggcacc
    17761 ttgaccagca aataaaaagg ctgttttagt catttcttac aactcctgcc cagcgagagg
    17821 cttcttcttg aattttctta gcggctccgt aatacaaatc ttttaggatt tcttcagctg
    17881 tttcttcttt agaaacaagc cctgcgattt gacctgccat gacagagcca ccatccacat
    17941 caccgtgaac aactgctttg gctagggcac ctgctcccat ttgttcaaag atttctaaat
    18001 caggatcttc ctgcttaaag gcatcttttt cagccagttc aaaatctcta gtcaactgat
    18061 ttttaatagc acgaacagca tgaccaaagt gctgagctga aatcgtagta tcaatatccc
    18121 ttgcttttaa aattttctcc ttgtagtttg gatgggcatt cgactctttt gcaactacaa
    18181 accgtgtccc cacctgtaca gcctctgcac ctagcataaa gccagccgca gcaccttcac
    18241 catccgcaat tcctcctgca gcaataacag gaatagatat agctgtggct acctgtcgca
    18301 ccaaggtcat ggttgttaat ttaccgatat gccccccagc ttccattcct tctgcaataa
    18361 cagcgtctgc accgattttt tccatgcgtt tagctaaagc gacactagga acgacaggaa
    18421 taacgattat cccagcttca tggaaacgtt ccatatactt gcttggattt cctgctcctg
    18481 ttgtgacaac tttaacacct tcttcaataa cgagatccac gatatcttcc acaaagggag
    18541 ataagagcat gatgttgacc ccaaagggtt tatcagtcaa tgatttgatt ttatcaatat
    18601 tggccttgac aacttctttc ggggcatttc ccccaccgat aattcctaat cctccagcct
    18661 tggaaacagc ccctgccaaa tcaccatcag caacccaggc catccctcct tggaaaatag
    18721 gatagtcaat cttcaataat tctgtaatac gcgttttcat agtgcctcca accttccttg
    18781 cttacgtaat agttcgattt aaccataatt tgacagtcaa actattacct aaacaagagg
    18841 gagtgggttt ctccctactc cttctactaa tattctgctt attttgcttg ctcttcaacg
    18901 taagcaacca agtcaccaac tgttttcaag tcattttctg cttcgatttg gatatcaaaa
    18961 gcatcttcga tttctgagat tacttggaac aagtccaatg aatctgcgtc caaatcatca
    19021 aaagttgatt caagtgttac ttctgatgcg tcttttccaa gttcttcaac gataatttct
    19081 tgtacttttt caaatactgc catgatagga ctcctttaaa ataaatagtt tttttataac
    19141 aatgtgttca ccacatgatt acctaaattg taagaatgag cgtgccccag gtcaagcctc
    19201 caccgaagcc tgatagaaga acagtctggc taccatctaa agggatgaga ccttgttcta
    19261 cacactctga aagtaaaatc gggatactgg ctgcactggt attgccatat tccatcatat
    19321 tggctggaag tttggctcgg tcaacaccaa tttttctagc catcttatcc aaaatacggt
    19381 cattggcttg atgaagtagc agataatcca agtctgtcac ctctatagga gattcatcaa
    19441 tagtctgctt gatagacttg gctacatctc gaatggcaaa atcaaagact gtgcgtccat
    19501 ccatcttcaa aaatgaatct gcactttctt gatctgaaaa tggagaatgc aaacctgaat
    19561 gcccataagt taaacactcg ctgcgacttc catcgctatt gagactctca gctaagaaat
    19621 gctcttgctc gctagcttct aacaagacac caccagcacc atctccaaac aacacagctg
    19681 ttgatcgatc cgaccaatcg actgccttag agagggtttc actaccaatc accaagcctt
    19741 tttgaaagcg accagaagcg ataaactttt cagcagttga aagagcaaat acaaatccac
    19801 tgcaagccgc ggttaagtca aaagcaaagg ctttattagc gccaatatta gcttgaacac
    19861 gagcagctgt agagggcatc atcgaatctg gagtaatggt agctaggatg ataaaatcca
    19921 gttcttctcc tgttattcca gcttttgcca tcagtttctt agcaacctct gtagccaaat
    19981 cactggtaga ttctgttctt gaaatatgcc tttgtcgtat tcccgttcga cttgaaatcc
    20041 actcatcatt ggtatccata atctgagcca agtcgtgatt tgtaaccact tgctctggca
    20101 cataatgagc aacctgactt atttttgcaa aagccattat ttcaaatcct ccaaaaattg
    20161 gtaaagatta gtcaagcctt tacccatgac agcaatttct tcctcgctca tgccatcaat
    20221 aattttttct accatggcct tgtggaagcg tttatgcagt ctatgaatca agcgaccctt
    20281 ctttgtcaaa tgcagatgca ccacacgacg atcctgttct gaccgaactc gctcaatgta
    20341 gcccttacgc tctaaattgt tcaaacttgt cgtaacagtt ccaagagtta ccatcaactc
    20401 ttttgacact tgacttggag tcacatctgg agccttaccg atgacatcaa tcgtatgcat
    20461 ttctttgatg gagatatcct taaaacgact acctctcaag ttcacttcct caattacaag
    20521 gacattgtta aatatagatg ttaaatattc attgattcgt tggtagtcca tttttcatat
    20581 ccctccttct tcaaaaaact tttacaatca aaacatttga cagaaagaat tatatcaaaa
    20641 ttcaaagaat gatgcaagta ttttttattt tcctgtaaat ttaggtcttc ttctctccga
    20701 atgagctcgc actccttctt tgaaatcctc tgtttgagct aaggatttct gtaggttcag
    20761 ttctaaagta gcgtaaccct gccagtcttt aaattggctc tcccaaacca acttcttaat
    20821 ggcagcatag gaattacttg aagcacgtct taatttttta agaagctgtt ctctcgtctt
    20881 ttcaagtttt tcagcttcag aaacgcggta aaccaacccc cattctaggg ctttttctgc
    20941 cgttaaagcc tctcctgtca tagctaattg agcagcacgc gtcacaccga tactgcggct
    21001 caagagatga attccccctg catctggagc caaaccaaca ccaacaaagg cttggataaa
    21061 cttagcctta tccgtcgcca aacagaaatc tgccgcaaca gccatattcg ctgcggcacc
    21121 tgcaacagca ccgtcaactt ccattaaaac aggtttggct atttgcttga ttttataaga
    21181 aatcgtattg accaactctg cgatttttgt caatgatgga atatcatcct catccactgc
    21241 ccgcttcatc tctaccaaat ctcccccaac tgagaagacc tttccattgg cattgattaa
    21301 gataaaatgc acagctggat tttcttctgc caaagtcaga gcttctaaaa tctcctcaca
    21361 catgggaata tgaaaaccat ttgcgacctc aggacggttc aaggtaagga ttgccaagtc
    21421 ctcttcaagt tgataaataa tgtgttccat cagggctcct ttttatttta taagcagttt
    21481 atactcttcg aaaatctctt taaaccgcgt cagcttccat ctgcaacctc aaaacagtat
    21541 tttgagctga cttcgtctag cttcttagtt tgctctttga ttttcattga gtgttaaatt
    21601 atttcactgt caaagtatat cttttttagg agcagaaagc aagaaaaaat cgactgaaag
    21661 tgtctcagcc gataagaaag gctcgcttta ggctagaaag catcttattt tcttcattga
    21721 aaaaagcctt tctttctgct ataatcgtat aaaatactta ctttaggagt tcttatgaaa
    21781 gttgttaaat ttggaggtag ttctcttgcc tctgctagtc aattagaaaa agttttaaac
    21841 atcgtcaaaa gcgattcaga gcgtcgtttt gtagtcgttt ctgcgcctgg taaacgcaat
    21901 gctgaagata ctaaggttac ggatgccctg attaaatact accgcgacta tgttgcgggt
    21961 aacgatatta gcaagagcca aagctggatt atcgaccgct atgctgctat ggttagtgaa
    22021 ttgggactaa aaccagctgt gctagaaaaa atttctaaaa gcattcacgc cttggccact
    22081 cttcctattg aagaaaatga atttctctac gatactttcc tagcagccgg tgaaaataac
    22141 aatgccaaat tgattgctgc ctactttaac caaaatggta tcgatgcacg ctatatgcac
    22201 cctagagaag ctgggattgt ggtcacaaat gaacctggtc acgctcgcat cattccatca
    22261 agttatgaca agattgaaga attgacaaac accaatgaag tccttgtcat tcctggtttc
    22321 tttggtgtca ctaaggaaaa tcaaatctgt actttctcac gtggaggttc tgatgttaca
    22381 ggttctatca ttgctgctgg tgtcaaagcc gacctctatg aaaactttac ggacgttgat
    22441 ggtatctttg cagcccaccc tggtattatc caccaaccac actcgattcc tgagttgacc
    22501 taccgtgaaa tgcgcgagtt ggcctatgca ggcttctcag tccttcatga cgaggctctt
    22561 cttcctgcct accgtggaaa aattcctctg gttatcaaga ataccaacaa ccctgaccat
    22621 ccaggtactc gtatcgttct aaaacacagt aatgatgaat ttccagttgt gggaattgct
    22681 ggtgactcag gctttgtcag cattaacatg tcgaaatacc tcatgaaccg tgaggttgga
    22741 tttggccgca aggttctgca aatcctggaa gaacttaaca tcggttggga acatatgcca
    22801 acaggtatcg acgatctttc tatcattctc cgttctcgcc aactaactcc catcaaggaa
    22861 gaagaaatcc tgcgtcagtt ggttcaaaag gctaaagtag atcatgcaga aatcgaacac
    22921 gacctttcta tcattatgat tgttggtgaa aaaatgaaga gccacatcgg agtgactgct
    22981 actgcgacac gcgctctatc tgaaaacaaa atcaacatcc agatgatgtc tcaaggttct
    23041 agtgaagttt ctattatgtt tgttgtcaat aaagaccaag agaaagcagc aattaaagcc
    23101 ctctacaatg ccttttttgg agaaagtaag gaagactaag catggcccaa tcacttaaca
    23161 aaacagtact cctcagtacg acaggcactt cctacctctc tatcgctggg aaagttggga
    23221 aattccttgt cggagatcag gctttggaat tttacccaga tgtcaatgtc gaacaattta
    23281 tccagattcc ttggagccat atcaatcaga ttggagccaa tgtcactgga cgcaaaatca
    23341 gccgccactt cgaagtcttt acagacaaag gaaaattcct ctttgcttca aaagactcag
    23401 gtgccattct caaaattgca cgcgaaaaac taggcaatga caaggtcgtc aaacttccga
    23461 ccctgattca gacaattagc caaaaattta aaaatctatt tgcaaaaaag tagaaacttt
    23521 agtataatca tagcaacgga taagtaggtt atcttcttct ttcagaaagt ctgcgggtac
    23581 tgcgagcaga taggagggat aggtgaaatt ctaccgttag taacaattac atacacaatc
    23641 aagtgcacac ctgtgaagtt ggatggaacc gtggccctgc cactccaacg ttttgtcagg
    23701 cgtgcttttt tcataaagga gttcttatgt tagatatcaa acgtattcgt acagattttg
    23761 aagctgtcgc agaaaaatta gctacacgtg gtgtagatgc tgctgtcttg aatgaaatga
    23821 aagaaatcga tgctaaacgt cgtaacatct tgatcaaggt tgaaactctc aaagcagaac
    23881 gtaacacagt ttctgctgag attgcccaag ctaagcgcaa caaggaaaat acagatgaca
    23941 agattgctgc catgcaaaat ctatctgctg agattaaagc cttggatgct gaattggcag
    24001 aaatcgatgc taaattgaca gaatttacaa cgactcttcc aaatatccca gctgacagcg
    24061 ttcctgttgg ggctgacgaa gacgacaatg tggaagttcg ccgttggggt actccacgcg
    24121 agtttgactt cgaacctaaa gctcactggg atctcggtga agaccttggt atccttgact
    24181 gggaacgcgg tggtaaggta acaggcgctc gcttcctctt ctataaaggc ctcggtgctc
    24241 gtttggaacg tgctatctac aactttatgt tggatgaaca tggaaaagag ggctatactg
    24301 aagtcatcac accttacata gtcaaccatg attctatgtt tggtactggt cagtatccaa
    24361 aatttaagga agatactttt gaactcagcg ataccaactt tgtcttgatt ccaactgctg
    24421 aagttcctct gacaaactac taccgtgatg aaatcttaga cggcaaagat cttccaatct
    24481 acttcactgc tatgagtccg tcattccgtt ctgaggctgg ttctgccggt cgtgatacgc
    24541 gtggcttgat ccgtttgcac caattccaca aggttgaaat ggtcaaattt gccaaaccag
    24601 aagaatctta cgaagaattg gaaaaaatga cagccaacgc tgaaaacatt cttcaaaaac
    24661 tcaaccttcc ataccgtgtc gttgctctct ctactggaga tatgggcttc tcagctacga
    24721 agacttacga cttggaagtg tggattccag cacaaaacaa ttaccgtgaa atctcaagct
    24781 gttcaaacac agaagatttc caagcccgtc gtgcccaaat ccgttaccgt gatgaagcag
    24841 atggcaaggt gaaactcctt cataccttga acggttctgg acttgcagtt ggacgtacag
    24901 tggctgcaat tcttgaaaat taccaaaatg aagatggttc tgtgaccatc ccagaagcac
    24961 ttcgtccata catgggtgga gctgaagtca tcaaaccata aaaaataagg tttagctatt
    25021 tctagctaga ccttttttcg taaccaaatc agataagcac ctagtacaaa gaataaaata
    25081 gttaggcata taatggtttc agccaatacc aggtaatcca gaaatggaag tttcaaaatt
    25141 ccctgagcca tcttgagcga ggtcgctgtg ataatggttg ggaaggtgag ggctgagaag
    25201 gctggttgaa aaccttgttt taaaatgttg ggcagacgag ttaaaacaaa gaaaaagaag
    25261 gattgagaag ccaaaatcat gacaatcaag acccaattcg gcaggctggt tcctcctact
    25321 cgaactagag aagccaagag tagagagaaa ggagcacagt agattccttc ttgtccaagc
    25381 aaggctagtg ggagtggatg tttctttaaa tcgctataaa taaggggata gagatagaag
    25441 gtcaagagaa aaccaaaact caaggtcgca taggcaattt cgataatacc taccagagga
    25501 taggtcaagg cagccactgc tatccccaca tagagaaccg tccaacttgg agtggcatga
    25561 accctccgcc ctggacaagc aaacttgatg gtaaaaccag caatcaaggt caaatccaag
    25621 agaaatgaaa accaccaaat cccttgtgct accaaaggaa gataagagaa tacgcgaaag
    25681 acataggtcg ataaaatcat cccagccata ggaaaggttg ccattcctga caaaagaggg
    25741 ggcttggtca attcttgctt ggtttctttc caattaaaga gatgcagaat tagaaagtaa
    25801 atccataaaa ccaaaccagt cagactaaaa agatgggata gaaccggcaa cgtatctaaa
    25861 ataagatttc cagctcctgc caaacctagc aaacaacctg aaaatactaa ggggagtttt
    25921 ttcatcctaa cctccaataa tcatgttagt ttcagtataa cataaaagcg cttaaatgag
    25981 gatttaaaaa aacgaagtcc gattatttca gacttcattt tactcagata tgaattaggc
    26041 ataaggttgc aattctggat taattggtgt attagctaag ttgttggcat agttacagag
    26101 gattgctagg ctgacaccaa aaaccacatc caaggcattt tgttgagtgt agccagcttc
    26161 taaaaactca gacaaggctt catctcctac acgacccttg gtattgataa ctgccaaggt
    26221 aaacttagct agggtatcca atttaggatc tgtttcaatt ggagtacgat tgcgaagagc
    26281 ttgaatcaag tcatcattca tctggatttg tttgatggaa aaggctgtgt gacctgcgac
    26341 acagaaggca caaccattgg tcacggctgc cgtgatttgc accacttcac gctcaacggg
    26401 tgtcaggctg ttgcgacggt ggatagatga gacaatttgg taggcttcta aaacagtcgg
    26461 ggcattggcc aagagaccga ttaggttggg aatatagcca ttgttgtctt tttctactgt
    26521 ttcaagaatt tctttcactt ctgctggtgc tgactctact gtatggatag taaatgttgt
    26581 cataagatac ctcttttctt attattgaca ctaatattat tggaaaatct tataaaatcc
    26641 tgattcctaa gtttatctaa gataaagctt tattctctca taagattttc gttgttatat
    26701 tagtttatca cacttccaat cacttgcata atatatatta tatatcaggc tgataaaaat
    26761 tatttatagg caaaaaaatc acacgagctg tgtgattcca ttatttgtca aaatactttt
    26821 tagtttcagc aataacgact tgcgacaaga ccaagagggc aatcaagttt ggcagagcca
    26881 tcaaggcgtt aacgatatct gcgataatcc agaccatatc caactcgata aatcctccta
    26941 acaagaccat gagcacaaaa accacacggt agagccagat aaagcgaacc ccaaagagga
    27001 actcaaaaca gcgttctccg taatagttcc aacctagaat cgttgtaaag gcaaaaagca
    27061 caaggaagat ggtcaagagg gcaggcccaa agtgtgaaaa gactgttgag aaagctgact
    27121 gagtcaaggc aaccccattc aagtcaccac tccaaactcc agttaccaag atggtcaaac
    27181 cagttagagt acaaatgatg agggtatcaa taaaggttcc tgtcatggaa atcaaacctt
    27241 gctctactgg ttcatttgtc ttggcagctg cagctgcaat aggagcagaa cccagaccag
    27301 attcgtttga aaacacacca cgcgccacac cattttgaat agccatccga acgctagcac
    27361 cagcaaatcc acctaccgca gcaaggggac taaaagctga ggtaaagact aaagcgattg
    27421 tgccagggat ttttccgata ttaaagaaaa taactgtaag agttcctaag atataaatga
    27481 tggccataaa aggaacaaca gtagttgaaa ccttagaaat agacttgagc ccaccaaaga
    27541 ctgcaatcgc tacaaagaca gacaagacga gagctgtgat ggctggcgaa atcgtcgttg
    27601 tattttggat agattctgta atcgagttga cttgggtgaa ggttccgatt cccaagagag
    27661 caaccaatac tcctgctact gcaaataaaa cagcaagtgg tcgccacttt tctcccatcc
    27721 ctagaaggat ataatgcatg ggacctcccg ctactgcacc atggtcgtcc ttggtgcggt
    27781 atttgatggc caagagtcct tccgcatact tggtagccat tccaaagaaa gccgccatcc
    27841 acatccaaaa tagagctcct ggtccaccaa ccttgatagc cgtcgcaact cctatgatat
    27901 ttcctgttcc aacagttgat gccaaggctg tacacagagc tgtaaaactg gatacatcac
    27961 catgtccctt atcctggata aaaataagct gaaaggcctt gggcagacgc aaaacctgca
    28021 agagtcctag ccgcatggtt aggtaaatcc ctgttccgac caataaaatc aagaggggcg
    28081 gtccccaagc aaaagcatcg attgatttaa gcaattctaa catttccttc tcctatcgtt
    28141 tcaaccccaa aagaaagagc acatgcaaga tacatgtact ctggaatgct tagataaatg
    28201 ctaaaaagcg gtctatccta gctctgtcct tttacctgag agtttgagca gttgcctgcc
    28261 ttgccccttc ggtgccttta cggtctctcc agagttccgt ccatttacag tcatggaaaa
    28321 tcaaacgatt ccccacttct attaaacttc attcggtgtt ggtatttaat tgattctaat
    28381 tttacaaaaa atgttggctt ttgtcaatgt gtttattagt aaaaattagt tcaacagttt
    28441 ttactttata aagtccagaa tactgctatc ctttaaaagt gacaatagtc gcaccactgc
    28501 ctccagcatt ttgtggggca tagccgaaac tcttgacatg tttgtttctt tgcaagtatt
    28561 tggtaactcc ttcacggatg actcctgttc cgataccatg gatgatatca acttgagcca
    28621 tattgttaag caaggcttgg tcgatgaagg catctagctc attcatggct tcttcatagc
    28681 gcttgcctcg aagatccagt ctagcttgag gtcctcgccc agaagttcgt ttcacaacat
    28741 tgacctgttt cttcttgact ggtttttctt gctgggcttg aacaagatca aactctttct
    28801 cttccaaggt catcttaatc aagccaactt gggcttccca gcgaccgtcc ttgagttgac
    28861 tggtcaaggt accacgctga ccataactga gaaccacgat atcatctccc acctttggag
    28921 ctcgtttttt cttggccttt tgaaggacct tatttttaga caagtccact ttttcaggag
    28981 ccaatttttt caacttggcc ttggcttcaa tgatttcgtg gggcttgagt tgggatttac
    29041 tgtggagatt tttgagaatc tggtcacttt cacttagggc catatccaca atctcagcag
    29101 cctgttcacg cgccttgtta agctcggttt ccttttcacg attaagctcg ttgtagagtt
    29161 tttttagcgc acggttcatc ttgagatttt cttgctccac ctcacggata ttgtccaagc
    29221 gtttgcggct ttccagcgtc tgctcttcta attgctcaat gatacgattg acgtcattgt
    29281 cctgatcgat ctgctgactg gcatctccta cgataacttc agataggcct agacgtttgg
    29341 caatttcaaa ggcattactt cggccaggaa caccctgcat aaagcgatag gtcgggcgaa
    29401 gagttgcagt atcaaactcc atactggcat tttgcacaaa ggctgtctca ataccgtagg
    29461 ccttgagttc tggatagtgg gtcgtcgcca tggtcttgat ttgacgcagg cgaaggtcct
    29521 ccagaatagc catggcaagg gctgctccct cttggggatc agtaccagcc cccaattcat
    29581 ccaaaagtaa gagtgaatgt tggttgacct tgccaagaat atccacgata ttggtcatat
    29641 ggctagagaa ggtagacaag ctctgctcaa tagactgctc atctccaata tcagcaaaaa
    29701 tttcttcaaa aataccaaca cgacttccct tgtctgctaa aatcggcaat cctgactggg
    29761 ccatgacctg tgtcaagccc agagttttga gcatgatggt cttcccacct gtattgggac
    29821 ctgtaatgac aatagctgtt aaatcttgac caaaatagac atcatttgcg acggcatttt
    29881 tgaccaaagg atggcagaca tggagcagtt gaatctcttg attttctgac agctgaggca
    29941 cgactgcttg tctttcttgg ataaatcgaa ccttggcacg aatcaagtcc agatgaccga
    30001 taatccaagc atcattagca atctcagccg catgagggcg gacacgctca gaaatttctt
    30061 ggagaatgcg aagcatttca tagcgctcat ctgctcgcag actagcaatt tcttcgctca
    30121 gtttgactac ctcacggggt tcgatataga cggtgtttcc actagcagaa atatcatgaa
    30181 cgacacctgc aatcttattg cggtaggtgt ttttgactgg taaaacctta cggccatttc
    30241 tgctagcaac aattccttcc gtcaacatct gcgctttttg cttgagcaag tcttgtaaaa
    30301 catcgcgtac ctgactctcg ctatcatgta tttttcgacg gattcgcgcc aattcttcac
    30361 tggcaaaatt ttcaatgaaa cccgcatcat taaaggcctg aagatttcct tgtaattgcg
    30421 gaaaatcatg taatttctca aaccaaaggg ctaattcttc caagctgaca ttttccagat
    30481 tggcgtaaaa actttgaagt tctcggctgg caagaagcac gcgtttcaag agtaggaact
    30541 cctcgatatt gagatccgct cccatctcca acctcttgca gactcctgca atttccttag
    30601 ttgagagaat agtaaaatgc ggttgctcga cgaaaagagc ctgcatttcc ttcatctcag
    30661 caaaagcctg tttgatttta tctgctttgg cagtcggagc cagttgtctc aattgctcca
    30721 agccctgctc ggtcaacaaa tgaggctcaa acaaggcctt gaccttatcg aactctaatg
    30781 tttctaatat tttcttattc atctttattt ccttgtcttt gaatgtctag gtgtggtagc
    30841 tttttacatt ctgatagatt caagtcaaat ctgtaaacaa agggctagga aaactcctgc
    30901 cctttttatc cgattaaatt tgtcacccag atttgtttga gccaactggt tgttatcggt
    30961 atgctctgga tgatgtgttt tgcgacgata ctcttttcaa gaggattttg tataactgcc
    31021 atggggatgg tcgccaagat tgtcaaggcc atttgcaaga caaataaggt caccaacatg
    31081 gacaagatac ctgctgaaac ttggaacaac ttaccaccca gttttttgct aggaatcaag
    31141 tgtaagagaa gaccaagcaa acgaccaatg ctatagacaa tcccaaatac aagcaagtag
    31201 ccgatacctg cataaaagac cttatccagc tgaaagagtt gatccgatgg gaaaaagaaa
    31261 gtcccctgac cttcctgcga atttgcataa gggagcaata aatggaattg ctctccaagc
    31321 cccttataaa actggccagc cataaaagcc gatgccatgg ctgaaatcag gtaataaatc
    31381 tgtaagagca ggcctctccg atagccgata taaaatcccc aaaccaagac caatagaaga
    31441 aggaatgaaa tcataaggaa tcctcaatct tgctctgttc ttgcttacaa gtcacaagct
    31501 tgtgacggag ttcttctagc tcttgctcct tatcgtcaaa ttcaatctca cggctgagct
    31561 gagttgataa acagttgact gccaacaaaa gagcgattgt ttcatcatct gcgctaggca
    31621 tttgttcttt aattgcttgg tatttttctg tcgcaacctt agcgatttcc tccataaaaa
    31681 ggttatcatg ttcgcttgtc aaggttaacg attttttccc gaatgtaaat ttgaatcgat
    31741 ttagatttgc cataaaattc acctcacgat attataccaa aattcgctaa ttttgtcagt
    31801 ttttacaaat tttactgctt ttgtggtaca atagaaacta tggcaagtat cacactcaca
    31861 ccaagcgaaa aggatattca ggcttttctt gaacactatc aaaccagtct ggctcctagc
    31921 aagaatccct atatccgcta ctttttgaaa ctacctcaag caacggtttc tatctatact
    31981 tctggaaaaa tcttgcttca gggtgaaggg gctgaaaaat acgccagttt ctttggctat
    32041 caagctgtag agcaaaccag cggacaaaat cttcctttaa ttgggacaga tgaggtggga
    32101 aatggttcct actttggtgg gcttgcagtt gtggctgcct ttgtcacacc tgaccagcac
    32161 gactttttac gaaaactcgg tgtgggggat tctaagactc tgaccgacca aaagatccgt
    32221 cagattgctc ctattctcaa ggaaaaaatc cagcaccagg cactccttct ctcacccagc
    32281 aagtacaacg aggtcatcgg aaaccgctac aatgctgttt cggttaaggt tgccctccat
    32341 aatcaggcta tctatctcct ccttcaaaaa ggtgttcagc ctgagaaaat tgtgattgat
    32401 gcctttacca gtgctaaaaa ttatgacaag tacttggcac aagagaccaa tcgtttcagc
    32461 aatcctatca gcttagaaga aaaggctgag ggcaaatact tggctgtcgc agtttcttct
    32521 gtcattgcgc gtgatctctt tctggaaaat cttgaaaatt tgggacgaga actgggttat
    32581 cagcttccaa gtggagctgg aacggcttct gacaaggtgg ctagccagat tttgcaagcc
    32641 tatggtatgc agggactcaa cttctgcgcc aaattgcact ttaaaaacac tgaaaaagcg
    32701 aaaaaacgct tagaaaggta agttatgaat tcatttaaaa atttcttgaa agagtgggga
    32761 ctgttcctcc taattctgtc attactagct ttaagtcgta tctttttttg gagcaatgtt
    32821 cgcgtagaag gacattccat ggatccgacc ctagcggatg gcgaaattct cttcgttgta
    32881 aaacaccttc ctattgaccg ttttgatatc gtggtggccc atgaggaaga tggcaataag
    32941 gacattgtca aacgtgtcat cggaatgcct ggcgatacca tccgttacga aaatgataaa
    33001 ctttacatca atgacaaaga aacggacgag ccttatctag cagactatat caaacgcttc
    33061 aaggatgaca aactccaaag cacttactca ggcaagggct ttgaaggaaa taaaggaact
    33121 ttctttagaa gtatcgctca aaaagcccaa gccttcacag ttgatgtcaa ctacaacacc
    33181 aactttagct ttactgttcc agaaggagaa taccttctcc tcggagatga ccgcttggtt
    33241 tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag atatcacagg ggaagctaaa
    33301 ttccgcttct ggccaatcac ccgtatcgga acattttaag agacctaaga ggccgagaat
    33361 caccaatctc agcctcttct tctatcgtga gaagtgattg tttattgaca aatactcaat
    33421 gaaaatcaaa gaacaaacta ggaagctagc cgtaggctgc ttaaaacagt gttttgaagt
    33481 tgtggataga actgacgaag tcagctcaaa atactgtttt gaggttgcag atggaagctg
    33541 acgtggtttg aagatatttt cgaagagtat aaaataaaaa caatcccaac tctcacctat
    33601 tcatgcaaag gaattttatg gaagtttatt tttcaggaac cattgaacgg attatttttg
    33661 aaaatcccag caatttttat cgcatcctcc tcctagaaat cgacgatacg gacgcagagg
    33721 attttgatga ttttgaaatc attgtcacag gaaccatggc tgatgtaatt gagggcgaag
    33781 actatacttt ttgggggcaa attgtccagc actccaagta tggagaacaa ctgcaaatca
    33841 gtcgttatga tcgcgcaaaa ccaactagta agggcttggt caagtacttt tcaagtagcc
    33901 atttcaaggg gattggtctc aagacagctc agaaaatcgt ggatacctat ggcgaaaata
    33961 ccattgacga aattttgcaa cacccagaaa agttagaagg catcgcagga ctctctgcca
    34021 aaaatcgcga ggctttcgtc tccactctcc gtctcaacta cggaacggag atggttttgg
    34081 ccaaactagc caactacggc attcccaaca aactagcctt tcagattcaa gacttttaca
    34141 aggaagaaac ccttgatgtg gttgaaaatt atccctacca gctggttgag gatatcaagg
    34201 gtttgggctt taccattgct gaccaactag ctgaggaact aggcatcgaa agtcaggctc
    34261 ctgaacgctt ccgcgccggt ctagttcaca gtctttttca ggcctgtatg gaaacagggg
    34321 acacctatgt tgaagcacgg gatttgctgg aacaaaccct tactctcctt gagtcttccc
    34381 gtcccgtgga actagacccc agccaagttg cccaagagct ctcctacctg atcgaagaag
    34441 acaaggttca gcagattgat accaagatct ttgacaacag cctctttttc gctgaggaag
    34501 gtatccgtag tcacttgatt cgtatccttg aaaaaggaaa acagaagagc caagatttag
    34561 aaactattca aaagcatatc actactgtcg agcaagaact ggggattgag tatgatagca
    34621 ttcaaaaaca agctatttgt gacgctatcc agaacaaggt ctttatcctg acaggtgggc
    34681 ctggtactgg taagacaact gttatcaatg gaatcatcgc tgtttatgcc cttttagaag
    34741 gacttgactt caggaagaaa agcaatctgc ccattcttct tgctgctcca actggtcgag
    34801 ctgctcgtcg catgaatgaa ttgacaggtt tgcctagcgc gaccatacat cgccacttgg
    34861 gaatgacagg tgacgatgat accagtcatc tggaagatta cctagatgct gactttatca
    34921 tcgtggatga attctctatg gtagatactt ggctggccaa tcaactcttc tccaatatct
    34981 cttctaacag taagatcctc atcgtaggtg acagtgatca gctaccttct gtcagtcctg
    35041 gacaggttct agcggatctg cttcatattc ctttgattcc tcagactcgc ttggaaaaaa
    35101 tttatcgaca aagcaaagaa tcaaccatcg tcaccctagc tagtcagatt cgacagggca
    35161 tcttgccagc tgatttcacc caaaaaaaag ctgaccgttc ctactttgaa attgctagtg
    35221 gccatattcc tgccaccatt gaaaaaatct taggtgtcgc cctcagaaat ggtattcctg
    35281 cccgtgatat ccaagttcta gctcctatgt accgagggac ggcagggatt gatgctatca
    35341 atcagctcat gcaagacctg ctcaatcccc cacaaaaaga tcaactcagt tttgaagctc
    35401 ctcagtgcca ctatcgtaag agagacaagg tcattcattt ggttaacgat gctgaaatca
    35461 atgtctttaa tggggattta ggagctatca cagacctgat tcctggtaaa tacaccgaat
    35521 cgaaacaaga cgagattgtc attgattttg atggtaatga agtctcttac ccccgtaacg
    35581 aatggtacaa gattcgcttg gcctatgcca tgagtattca taagtctcag ggaagtgagt
    35641 tcccagttgt catcctaccg attactagtg ctagccgacg tatgctggag cgaaatctca
    35701 tctacacagc cgttacacgt gccaaaagca aactcatctt actaggcgaa ttacaagcct
    35761 tcgactatgc tacccaacac atcggaactg cccgaaaaac ctatctgatt gaacgcttca
    35821 gtgatctact ggagaatgtt gaagaaaagc aacaagctgt ctctgaaaca gtcacatcaa
    35881 gtgcctctga acaatcctac atcctaaccg aagaaaactg ggacagcatt ccagccatga
    35941 ttgggattac agacacagac ctcaaagaga tttttggaaa atagtacatc agaaaaccca
    36001 ccaattcagg tgggttttct gtttattaag attattttac tgttgctgtg cttgtgatca
    36061 attcaacagc ttttttgata gtgatatcat gtttcaacat gtcagctgaa agcaagtttt
    36121 gaacttgtgc aacttccatg ttgtagtctg ctgccaattg ctcaacttct ttttggattt
    36181 cttcttctga agcatcaaat ccttcagctt tggcaactgc ttcgataaca aggttagtct
    36241 tagtacgtga ctcagcttct gcttggtatt ggttgtgaag gtcttcttga gtagttccag
    36301 tgatttggaa gtacatgtca gggttgatcc cttgacgttg caagttccca aggaattcat
    36361 ttactgaacg gtgaacttct tcatggatca tttcttctgg aagttctacg atttcagcgt
    36421 tttctacagc tgtatcaatt gctgcacctt caactgcatc tttgtaagct tcttctttag
    36481 cagcagccaa ttctttgcgg tatttttctt tcaagtcagc aagtgtttca acttcttcat
    36541 caatgtcttt tgcaagttca tcgtcaagag ctggaacttc tttagctttt acttcgtgga
    36601 tagttgtcac gaatttagct tctttacctg caaggtcttc tgcttggtag tcttctggga
    36661 atgttacgat aacatcaacg gtttcgccag ctgagtgacc taccaattgg tcttcgaaac
    36721 cagggatgaa ttgacctgaa ccaagtccaa gtgagaagtt ttcacctttt ccaccgtcaa
    36781 attcaacacc gtcgatagaa ccaacgaagt cgatcacaac agtgtcgccg ttttcagcag
    36841 cagcttcctt gataaccaat tcagccagat tgttgcgttc gcgttcgata cgctcttcaa
    36901 catcagcgtc agttacttct ttttctacat caactgatac ttcaaggttt ttgtagtcac
    36961 ccaattttac ttcaggtttt gtaacgactt cagcagcgat aacccagtct tgaccttttt
    37021 ccattgaagt tacgtcaatt tttggttggg caaccacttc aagaccagct tcttttacag
    37081 ctgcttcata agcgtttggc aaaagagcgt tcataacgtc ttggtaaagt gattcttcac
    37141 caaatttttt gtcgaagata ggacgtggaa ggtgaccttt acggaaacct ggaacattaa
    37201 gagatttctt cactgacttg aagacacggt ccaattctgg tttgatttgg tcttgagaga
    37261 tagtgaaagt caagacacca cggtttgttt ctttgttttc aaatgataca gacattctgt
    37321 catttctcct taaaattttt taaatacagt ctattataac attttctata aataaaatct
    37381 agactaatgc agccaattat agacaacaag cctttttcct aactaaaaca ctcataaagt
    37441 atagacaatt ttagactact ttggatatct tttggttagt cattttccaa aactaattta
    37501 tctaattttt cagttatata gtagattgaa actagaatag tacacctcta cttctaaaac
    37561 atttttagaa atcgatttga ctgtcctgat caatttgtcc tgttcttgtt tcattttact
    37621 atatagatcg cattcgaaag aattcgttcc aaaattgctt ggttcgttct tctcttggac
    37681 gactaaagaa ttgatgggca ttattttttt caacaatttt cccaccgtcc ataaaaataa
    37741 catgatgggc tacatctcta gcaaaaccta tctcataaag ctgatcaaaa actcaatctg
    37801 aggagaaaat ccaataaggc tttccataat aaaggacatc ctatcaagat ttttgaacac
    37861 ccgataggct gcctttttcc gattttgaag actttttgtc cagcttcttt caccttataa
    37921 ataacaacaa aaatcaaaga caagctagaa agattaccgc agataactca aacagtgttt
    37981 tgaggttgca gacaagactg acggagtcag ctcaaagcac tgctttgagg ttgtagatag
    38041 aacaacatat gcctaagaca aagcgatgct aacctgattt gaagagattt tcgaaaaaga
    38101 ttaaatatac tctacaattt gctcaatcaa ttcttggtcg tctaaacctg taacttgtat
    38161 aacaacatct ttgactgatt gtttagccaa caattcacct aatcgaatac tttcttcatc
    38221 atttacatcg cgatagtcaa aggcatagcc aactgtttta agtaggtttt tatatgacaa
    38281 actgagttct ttcaattcac gtatcggccg gatgaatcgt tcattatagc ctaattttcg
    38341 gattggagta cgagctacgc gactaacctc gtccactatg aaagggtttt caaaccgttc
    38401 tataatgact ttgtgataat tctccaattc ttttttatca aagttccatt tggcaatcaa
    38461 gagactccga atttcagcta atacagattc aatccgagat ttaatattag gattttgaag
    38521 agcttccaaa attgtcttgg caccataatg cgcaccaatg taagctgaag ttgcatgtcc
    38581 agaattgact gaaaaaagtt ttcgctcaat aaagggttct aaatcttctt cataatgcac
    38641 atcttttaga cgtaaatctg gatttttaag acgcttggtt tccacgaccc attcattaaa
    38701 gggctcgacc acaacaaaaa gggaatcttc gtgactttgt gctggaacaa tcctgtctac
    38761 tgcagcattt ggaaaaccta tgtagttatc agcaaatgtc aaaccttccg gacttaaata
    38821 tttcttgact tcttgataaa gaaattgaga cccgccaatc atattttcac aggccataac
    38881 atccaatgcc tgtgtatttc ctgcaactcg gcgagcttcg attcctttgg ctagaagttc
    38941 ggcgataaaa gggagtatat taggtccgat tgcagtagta ataatatccg tcttttgaat
    39001 cgcttcaatg acttgctcag gatgttcttt gctattaatg ccagccacat tagttacttc
    39061 tatacgagac tgtcctttct gtgcaatttc aatttcatac ttgccctttt catttagagc
    39121 atgaattatc tgattattga catccacaaa atcaatatgg aaaccatttt taaatagaat
    39181 ttcacctata aaaccacgac cgatattacc ggcaccaaaa tgaacagaat gtttcattca
    39241 atttctcctt ttcctatttg agggacatat atactagatg acaaatgacg aaattcaatt
    39301 attcaacagc atcaaataag cgcaatactt cctcttttga ctgagcatct gctagtttaa
    39361 gaacattatc tacatctgca cagaagatag aaattttctg aataatttct aagtgttcat
    39421 taccaatacc agcaatacca aaaagaaccg ttgccacttg agggttagat acattcccaa
    39481 aatcaacccc tctagggact tgaacgactg taataccaga cttgagtaca tccttttttg
    39541 ctgcatctgt tccatgcggt atggcgataa agttacccat gtaaacagat agctctttat
    39601 ctcgctcaat catggcttca atatagtctt cattaacata tccaccctca taaagaactt
    39661 gcccacaata acaaatagct tcctccttgt tagaaaattg tttattaagc ttaatcaaat
    39721 gtttttcaag tttcatatta gttctccaat ttcttaattt tttcgttaaa aatagtattt
    39781 agcatctgat aaataatgga ttgatttccc gtcttgtaaa tctctgtata aagatgattt
    39841 tcaataatcg actgactaat agctgtcatt aaatctctcg cttcctcgct agcttcttta
    39901 gacattagca ttagcagaca ccttttgacc tcttcttcct ctcgtttcat tgacaatgcg
    39961 gagataggta gtttgagttc aaacatagta aaactatttg ttgttacttt gctagactga
    40021 ctatgtaaaa gcaccagacc agtattagga atagccatag gactattctg gaagtgagat
    40081 aacaattttc ttgttaggta atctctgtca ctcacattct tctgatattg gatgatttgt
    40141 tctaccgtgt gctcaaatga ttgattattc tccaattgaa ccaactcaaa ttgactcaaa
    40201 agctgactac tagatattaa ataatcttgc aagtcataac ttttctctgg agcgatagca
    40261 tcacgcgcga caattgtacg atgtgcctgt acatactgaa gtcgttcctg taattcaagc
    40321 aattctctga cggttggaaa actagaaatt acatcgattt cctgattagt cagcggcttg
    40381 gtagataaaa tataatcata ctgactgaga tcaatactgt ggtaatctac tagagactga
    40441 atatctatca actctacaaa aggagcaaca ctcttaattt tactgactaa taaatctcta
    40501 gtgacccgac gttcatccgt taaaagcaaa acacggactg gataaatctc tggactacgg
    40561 cctaagctag agataaaatg tagggcaatc atgccatact catactctgt atgaagatat
    40621 ttcggaaaaa tatcattcac taaaagactg atgactgtat aaagaaattt attcctttca
    40681 actaaaatct ggacagattc tggcaaattt tcaccctgaa aaaggatagg gacgcctaaa
    40741 ctgagccgaa tatgatggaa aagaaaattg aataaaacct tgtccttaaa aaagtcaatc
    40801 ttggtataca tggaaaccgt atcaatcaga tttgaaatat tgtagaaaaa ttcaccatca
    40861 aatttctccg taaagagcgg attgtcctga cgtttaatga ttaattcatc caagatgctc
    40921 gcaaaataga taatttcctg aatactataa aattgtgcag tctgcttaga gtaagcttga
    40981 aaaatttttt gagaaatttc caaagcctgc ttactagtat taggtgaatt ttcaatgttt
    41041 tgctcctgac ctataagaga taacaagatc gcaaaaaaca tcttcatcct agcatccata
    41101 tctggaaaac ctgacagttg cttattaaca atctgactgg ccagcctagt tctatctgct
    41161 tctaaaatat caaagctccc aaaattaccg gttgaaaaat ctgctaccga gatacagttt
    41221 gtcagtaaaa tagccaaaaa tcttctctta ccaactgaat caccagaaat ccgataacct
    41281 ttttgtcgtt caattttcag atcaaagtct aaaagacgct tatcaatatc tgaaatatcc
    41341 tgaataatag ttacattact gactttcgtg cactcttgca atgcttcatt ggtgacaaaa
    41401 ccactctcag tcagaaggcg ataggtaatc aactcttgtc tttcttgggg actatactca
    41461 actaacactt caagttctgt cggcaaatca tccaactctc cagtcaaaat atagtatttc
    41521 ccatgcttcg ttatttcgat tccatattgc tccatgctat ctgtcaaatc tgataaagtt
    41581 cgataaattg ttctagatga aacctgtaag atttcagtca tatcttgcat tgaaagcttc
    41641 cctacatgta ggaaagcctt caataattgt tcttctcgtt tggttaatag cattcgtaat
    41701 ttagaaacct ctagttctat ttgtacattc tagcagccat tttgtcatat tctggtgttg
    41761 ttactaaact atccacaata agaaattgag aattcggtgc tgcttgctgc acttctgcct
    41821 gtaaagaaat agttgttaca atcattatgt ttttagaatt aaattcacct aattctgaaa
    41881 ttttggcagt agaaactgga atacgaatat tcttgtttct aaagatagcc cgaatcgttt
    41941 cacagcccat agttgcagtt ccctgtgctt taccataagc aactactaca gcatcaatat
    42001 ggttaaggtc aatttcctga ctatctactg gtgctgaagt tggtatatct ccttcaattt
    42061 ctgctattgg agaagctcct gttaatgaag ctacaatttc atcataacga ggggacgcta
    42121 agaaattatc aacagaaaca tgaatagcac ttggactctt gtctttagct cttggtgtca
    42181 gttcttcctg agtaacaatt aatgtttttg gtgtatcaag caaatttgag attgcctgat
    42241 tagatactgg aatctctaga cctgcttttt taaccttatc tcgaagaata ctagctccca
    42301 tagcagagct tcccatacca gcatcgcagg cgaaaatgat tttttccact gagtctgtcg
    42361 aaacaactgc atcaacagaa gttgatacta actgaccttt agactgagcc ttagctgctt
    42421 gggtagccgc ctgagcagct tcgagcgaat cctcagttga cttgtctgca tgaagaataa
    42481 gggctgctac aaggaaagaa acaactgctg ccactaaaac acctaaaaga acatttagat
    42541 gaggccaaac accttttggc gccgtagcca taatcgcaat aatagaacct ggtgaagctg
    42601 gagatttaag accagcgtct aagagttgaa aagtaaaagt tccagagata cctcctgcca
    42661 tagcagctaa aaatagagta ggcttcatca taacataagg aaagtaaatt tcatgaatcc
    42721 ctccgaagaa atgaataacc attgcccccc aagaagaaga tttagcagaa cctttaccga
    42781 atacagcata agctaataga attcccaggc ctggtccagg attagcttcc aataggaaga
    42841 gaattgactt accagcttga gctacctgtt ctactcccag aggagtaaaa atgccatgat
    42901 tgagggcatt attgaggaaa aggactttag ccggttcgat gataatatta gccataggaa
    42961 ggaggcgagc attgacaata gcctcaacac cattcccaac agctccagta agagtcgata
    43021 cgactggacc gattgcgtag aaagccaaaa gcaataatgc aaaaccaacg agaccagctg
    43081 agaagttatt aactaacatt tcaaatccgg gacgaatttt ttcctggaac ttctcatcaa
    43141 atttcttgat agtccatcct cccagtgggc ccattaccat agctccgata aacataggaa
    43201 cactagaacc tgtgattgca ccaacagtag caatagatcc tacaacggca ccacgttggc
    43261 catggatcat atatccacct gtataaccaa tcaggattgg caataaatac gttaacatag
    43321 gaccaacaac agtagctaac tgttcatttg gcagatagcc atcagcgata aaaagggcag
    43381 tcaatactcc ccaagcaata aatgctccaa tattgggcat aaccatattt gaaagcgatg
    43441 tccctagttt ttgaaccctg actttcaatg atactttttc ttccattaga tcatcctcct
    43501 tttcttaaaa ctagtatata caaagttaaa aacctttaaa actaaagttt tgtcacagat
    43561 tttgtgtcaa aacattactt ttgacgaggc tgggcaaaaa ggc
//
LOCUS       DAVGJJ010000007        41244 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000007 DAVGJJ010000000
VERSION     DAVGJJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.007"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            543..2222
                     /locus_tag="VVE35_000355"
     CDS             543..2222
                     /locus_tag="VVE35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU3060611.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGSNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLHEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(2262..4346)
                     /locus_tag="VVE35_000356"
     CDS             complement(2262..4346)
                     /locus_tag="VVE35_000356"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU3060612.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHPQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMTPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            4517..5797
                     /locus_tag="VVE35_000357"
     CDS             4517..5797
                     /locus_tag="VVE35_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU3060613.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            5888..8533
                     /locus_tag="VVE35_000358"
     CDS             5888..8533
                     /locus_tag="VVE35_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU3060614.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEPL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            8617..9486
                     /locus_tag="VVE35_000359"
     CDS             8617..9486
                     /locus_tag="VVE35_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3060615.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHSELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            9480..11360
                     /locus_tag="VVE35_000360"
     CDS             9480..11360
                     /locus_tag="VVE35_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU3060616.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            11540..11823
                     /locus_tag="VVE35_000361"
                     /pseudo
     CDS             11540..11823
                     /locus_tag="VVE35_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902838.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11860..12634)
                     /locus_tag="VVE35_000362"
                     /pseudo
     CDS             complement(11860..12634)
                     /locus_tag="VVE35_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(12579..12761)
                     /locus_tag="VVE35_000363"
     CDS             complement(12579..12761)
                     /locus_tag="VVE35_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU3060617.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            13074..14939
                     /gene="pcpA"
                     /locus_tag="VVE35_000364"
     CDS             13074..14939
                     /gene="pcpA"
                     /locus_tag="VVE35_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU3060618.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNRGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKD
                     KGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWV
                     KDKGLWYYLDESGSMATGWFTVSGQWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
     gene            complement(15418..15567)
                     /gene="rpmG"
                     /locus_tag="VVE35_000365"
     CDS             complement(15418..15567)
                     /gene="rpmG"
                     /locus_tag="VVE35_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3060619.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(15583..15765)
                     /gene="rpmF"
                     /locus_tag="VVE35_000366"
     CDS             complement(15583..15765)
                     /gene="rpmF"
                     /locus_tag="VVE35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU3060620.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            complement(15917..16117)
                     /locus_tag="VVE35_000367"
     CDS             complement(15917..16117)
                     /locus_tag="VVE35_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU3060621.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(16120..17127)
                     /locus_tag="VVE35_000368"
     CDS             complement(16120..17127)
                     /locus_tag="VVE35_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU3060622.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            17318..19348
                     /locus_tag="VVE35_000369"
     CDS             17318..19348
                     /locus_tag="VVE35_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU3060623.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            19352..19636
                     /locus_tag="VVE35_000370"
     CDS             19352..19636
                     /locus_tag="VVE35_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3060624.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            19649..20995
                     /locus_tag="VVE35_000371"
     CDS             19649..20995
                     /locus_tag="VVE35_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU3060625.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            20999..21856
                     /locus_tag="VVE35_000372"
     CDS             20999..21856
                     /locus_tag="VVE35_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU3060626.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRIKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            21853..22785
                     /locus_tag="VVE35_000373"
     CDS             21853..22785
                     /locus_tag="VVE35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU3060627.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            23018..24721
                     /gene="ilvD"
                     /locus_tag="VVE35_000374"
     CDS             23018..24721
                     /gene="ilvD"
                     /locus_tag="VVE35_000374"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU3060628.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(24930..25271)
                     /locus_tag="VVE35_000375"
     CDS             complement(24930..25271)
                     /locus_tag="VVE35_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU3060629.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            25395..26246
                     /gene="rgg"
                     /locus_tag="VVE35_000376"
     CDS             25395..26246
                     /gene="rgg"
                     /locus_tag="VVE35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU3060630.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATRGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEELLFLARGFQYDTDSELRKEITDVLKPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            26439..27668
                     /locus_tag="VVE35_000377"
     CDS             26439..27668
                     /locus_tag="VVE35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU3060631.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFFDNS"
     gene            27765..29054
                     /locus_tag="VVE35_000378"
     CDS             27765..29054
                     /locus_tag="VVE35_000378"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU3060632.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            29347..29661
                     /locus_tag="VVE35_000379"
     CDS             29347..29661
                     /locus_tag="VVE35_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060633.1"
                     /translation="MWVLVFILFIIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            29671..29877
                     /locus_tag="VVE35_000380"
     CDS             29671..29877
                     /locus_tag="VVE35_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3060634.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            29877..30074
                     /locus_tag="VVE35_000381"
     CDS             29877..30074
                     /locus_tag="VVE35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060635.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            30077..30811
                     /locus_tag="VVE35_000382"
     CDS             30077..30811
                     /locus_tag="VVE35_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU3060636.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            30823..31464
                     /locus_tag="VVE35_000383"
     CDS             30823..31464
                     /locus_tag="VVE35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3060637.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            31482..31712
                     /locus_tag="VVE35_000384"
     CDS             31482..31712
                     /locus_tag="VVE35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060638.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            31748..31828
                     /locus_tag="VVE35_000385"
                     /pseudo
     CDS             31748..31828
                     /locus_tag="VVE35_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            32064..33827
                     /gene="aspS"
                     /locus_tag="VVE35_000386"
     CDS             32064..33827
                     /gene="aspS"
                     /locus_tag="VVE35_000386"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU3060639.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGATDNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            33805..34746
                     /locus_tag="VVE35_000387"
     CDS             33805..34746
                     /locus_tag="VVE35_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU3060640.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(34866..34946)
                     /locus_tag="VVE35_000388"
                     /pseudo
     CDS             complement(34866..34946)
                     /locus_tag="VVE35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(35082..36068)
                     /locus_tag="VVE35_000389"
     CDS             complement(35082..36068)
                     /locus_tag="VVE35_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3060641.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(36078..36878)
                     /locus_tag="VVE35_000390"
     CDS             complement(36078..36878)
                     /locus_tag="VVE35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU3060642.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(37021..38187)
                     /locus_tag="VVE35_000391"
     CDS             complement(37021..38187)
                     /locus_tag="VVE35_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU3060643.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKATLTIIKSRLNNYLL
                     PAFGNYRLDKLTPPIIQHQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKAKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVNKTLNCEKEVTTPKTKSSIRVIDLDNKTVLMLRLY
                     KNRQAQVGREIGLTYEKVFSNSFDEYRDARALRSRLEKHLKLSDCPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            complement(38379..39353)
                     /locus_tag="VVE35_000392"
     CDS             complement(38379..39353)
                     /locus_tag="VVE35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEU3060644.1"
                     /translation="MKPFQTLDEQIKLLQSRGLEIDNIEECKRYLLTNNYYNVVNGYS
                     KFFQTSKDKFITGADFREIAATHFYDKEIKSAFLKAIIDAEKHFKSVLAYCFSEAYPT
                     PYAYLDINNFETQKDTKRLAQITNLISILARILNDYNRDKQNNSIKHHYKQHGVVPFW
                     VIINELTLGQAFNFYRNLNTDIKNQIARDLSPFLQENIEYIQNRPSKDLLSGKALESI
                     IKNILEIRNITAHNNKLFNYKCRENLPQLAYFHYYNNNANTSRQSVYYVFLALQCLLA
                     STQYAQLHNTIIKRTKVLNKKAHSIEAGIVLDTLGFPNNWYNITDTLR"
     gene            complement(39553..40221)
                     /locus_tag="VVE35_000393"
     CDS             complement(39553..40221)
                     /locus_tag="VVE35_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEU3060645.1"
                     /translation="MNYAQDYLDDILVRIAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLNSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGVEFQTASPAETPYLMTQWADNTAYRLNHAQNEKEILEILADTHIQIEGIHPFSD
                     GNGRTGRLLLMYLAMKYLNAPIIVSKDSRAHYMELLANQDVTGLADLFKESLDYETTR
                     KGQF"
     gene            complement(40309..40848)
                     /locus_tag="VVE35_000394"
     CDS             complement(40309..40848)
                     /locus_tag="VVE35_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060646.1"
                     /translation="MKKLLSTSAILLSATVLVACSNNQSATKDSSEQPKTEQKNTTST
                     NTKAKVDNSKYDDLISEIKLKLDPESTGAISVKIQNNVIDSDSSEPHDTIMILLTGTA
                     KDSAKETMDAINSNSATTNQQNAITVFRMSISEFAKKLPDDNTTLSLGYEKSANQYDL
                     IAKSSKQKDIIPIGELIVN"
     gene            complement(40892..41161)
                     /locus_tag="VVE35_000395"
     CDS             complement(40892..41161)
                     /locus_tag="VVE35_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEU3060647.1"
                     /translation="MGFFDTVKQEGSFSTASGVNGLHYVVLQVTLKEKFFGTGSGNLT
                     ELEDVINKQASKGYRLHTITTANGGSKGLGGGDRIQATMVFEKIL"
BASE COUNT        11944 a         7474 c         9017 g        12809 t
ORIGIN      
        1 ttttttaaaa aatttttcaa aaaatataca aatgaccaaa aagtgtccaa ttatcatctg
       61 gttatcactt ggatctgtat aaagagaaat gtttgtgtga aaggataaaa agatgcaaca
      121 ttcttttgaa gatagatttt ggagaagcat ttcttgttca attgtacaaa ttatagaatc
      181 ttttctgagt atttaggaag agataattat cttattgacc ctctttttat tttttgttat
      241 agtcgacgag gtgatagttt agaataatca cttattcaca atctgtggaa aagtctggat
      301 agtgaatggt cttcacacaa tgacctgaaa gaagcctgag aataattatg gagagtagca
      361 ttctgagagg tgttagcaga accatatgac agagctgttt gaagagggaa cattgaggag
      421 aaaaatcctg agcctaccag ttggagttgg aaagagctgg ctgttagatc atggtttatt
      481 atccacagcc tgtggataac cttgtgaaca agagaagttg ctaaagaagg agatatataa
      541 cgatgaagaa aatcaaaccg catggaccgt taccaagtca gactcagcta gcttatctgg
      601 gagatgaact agcagctttt atccacttcg gttctaatac cttttatgac caagaatggg
      661 ggactggaca ggaggatcct gagcgcttta acccgagtca gttggatgcg cgtgagtggg
      721 ttcgtgtgct caaggaaacg ggcttcaaaa agttgatttt ggtggtcaag caccacgatg
      781 gctttgtcct ttatccgaca gctcacacag attattcggt taaggtcagt ccttggagga
      841 gaggaaaggg cgacttgctc cttgaagtat cccaagctgc cacagagttt gatatggata
      901 tgggggtcta cctgtcaccg tgggatgccc atagtcccct ctatcatgtg gaccgagaag
      961 cggactacaa tgcctattat ctggctcagt tgaaggaaat cttatcaaat cctaactatg
     1021 ggaatgctgg taagttcgct gaggtttgga tggatggtgc cagaggagag ggcgcgcaaa
     1081 aggttaatta tgaatttgaa aaatggtttg aaaccattcg tgacctgcag ggcgattgct
     1141 tgattttttc aacagaaggc accagtatcc gatggattgg caatgaacga gggtatgcag
     1201 gtgatccact gtggcaaaag gtgaatcctg ataaactagg aacagaagca gagctggact
     1261 atcttcagca cggggatccc tcgggcacga ttttttcaat cggagaggca gatgtttcca
     1321 tccgtccagg ctggttctac catgaggatc aggatcctaa gtctctcgag gagttggtcg
     1381 aaatctactt tcactcagta gggcgaggaa ctccactctt gcttaatatt ccgccgaatc
     1441 aagctgggct ctttgatgca aaggatattg aacgacttca tgaatttgcg acctatcgca
     1501 atgagctcta taaagaagat ttggctctgg gagctgaggt atctggtcca gctctttccg
     1561 cagactttgc ttgtcgccat ttgacagacg gccttgagac cagctcttgg gcaaacgatg
     1621 cagacttgcc catccagtta gaactcgact taggttctcc taaaactttt gatgtaattg
     1681 agttaagaga agatttgaag ctagggcaac gaatcgctgc ttttcatgtg caagtagagg
     1741 tggatggtgt ctggcaggag tttggttcgg gtcatactgt tggttacaaa cgtctcttac
     1801 gaggagcagt tgttgaagca cagaagatac gtgtagtcat tacagaatca caggctttgc
     1861 ctttgttgac caagatttca ctctataaaa ctccgaggtt atcacaaaca gtagctgttc
     1921 aggggctagc atttgcagaa aaaagcctag ctgtggcaaa gggagagact cttcatttta
     1981 ggattgaacg tagtgaaagt agctcctctt tagaggctaa gatttcgatt caaccgggga
     2041 caggtgtcca tggtgtcgcc tatcaggatg agattcaagt ccttcaattc caagcagggg
     2101 aaagcaaaaa agatttgcat ctaccaaccc tgtatttcgc aggagataaa accttggatt
     2161 tctatctgaa cctaacggtg gatggtcagc ttgtggatca acttcaagtc caagtttcat
     2221 aaaagaagaa cctttgcgcg atgcaaaggt tcttttggtt attagtgact tggtaaccag
     2281 ctgagggtga aagttagttg ttcagctttt aggaggtctt ggtgttgaat agttgatacg
     2341 agtgttttgt ccagtcggca ttctttgaca aagttaaaat ggctgtggtt ttgctcagta
     2401 tgaatatcta gccatttatt ttctttagct aggtagactc gtagatggtc aaagagagga
     2461 attccgagat catagcttgg ttttcctgga caggttggat aaaatccgag agctgaccag
     2521 atgtaccaag cagagagact accattgtct tcatctccag gataggcttc ccaacttggg
     2581 tgaaaagctt tctgacggag cgtcttgata agaagggcag tgtagtcagg gtaatcgctg
     2641 taacggaaga gataaggaat gtggaaacta ggctggttgg aaatggctat ttgtccaaaa
     2701 ggagcagtag ccatctcgct catttcgtga atttcgtaac catagcctgt tgtttcaaag
     2761 aggggagcat cttgacaggc tttcaaaaga tagttgctaa aggtttcttt tccacccatc
     2821 agttggatta agccagggat gtcgtggaga acgcctaaag tagcttgaat ggcagagcat
     2881 tcagcgtagt ctcgccccca actataagga gagaagtcag ggtgaaagtt tccttgattg
     2941 tctcgtgctc gcatgtaacc tgtctcagcg tcaaatagct ggcggtaatt ttgtgaagca
     3001 gccttgtagg tttcagcgat ttctatgttc tctagttttt tggcacagct ggcgatacaa
     3061 aagtcactat aggcatagtc tagagtatgg ctaacacttt cgtggtggtc ggtagagagg
     3121 taacctagtt cttggtattg ggctagtccg tggcggccat tgattccgag agggtcggct
     3181 ttgctggctg tttcgagcat ggcttggaag agttctcctt ctaggtcggg ggtcatgtcc
     3241 ttgcaggcgc tatctgcgat aataccgtct aaaagtgtac ctggcatcat accccgttca
     3301 tctggagcca gccattttgg aaggaaacca gtatcgcggt agctattgag gaaaccttct
     3361 aaaaagcgtt gatagtgctc cggtatgata agggcaaaga gggggaaggt ggtgcggaag
     3421 gtatcccaga aaccattgtt gctaaagagg acaccaggct tgacagtacc agtagccaga
     3481 tccatgtgga tggcttgccc tgattcatta atctcataaa aagtctgtgg gaagaggaag
     3541 agtctgtaga ggcagtggtc aaagaaggtt cggtcagcct ctcctgtctc tataatgtca
     3601 aaacgatgga ggagattttc ccaatccact tgggcacttg atttacagct atcaaaatct
     3661 tcttgaggta gattgattag agcttgagaa ggagagatga aagaagtggc tagttgcatc
     3721 tcggtttgac tacttgctaa gtcaattcgc cagtctccag cttcttggct gatggcaaga
     3781 atatccgtgt tcatttgcag ggcagtgaac atcgttagcg aatttttgtt agtttcagtt
     3841 ttaccttctt gtcgcagggc aagagtccgc ttatctactt gctctactgt cagttcatct
     3901 gctgcgtgaa gatagaggga gagggctttg ccttgctttt gattcaaacg aatagaagca
     3961 ccatagcaag tcggtgtgag ctgggtttca atctgataac gcagagaaaa gagcttcaaa
     4021 tagtgaggct ggaagcaagc tttatctata tcataagaag actgggggtg aaagaggctg
     4081 tctcccccca gttgactggt gacaggtgtc agaaggagcc aagagtagtc cccaatccaa
     4141 ggactgggct ggtgagttaa tcgaatcccc tgaaagatag gcagatgcgg atcaaaaaac
     4201 caagatccat cctggtcact ggtctggggc acaaagtaat tcatcccaaa aggcacgcct
     4261 gtgtatggca gggtatttcc ccgagaaaag gcatgcttgt tggtagttcc aaagcgggta
     4321 tcgatggttt caagtagtgg tttcatagtc tttcctttag ctgtttttct acattatatc
     4381 agaaaaagag ggcctttaga aaaggagttt caaaaataac tattttgtag aaaaatgact
     4441 atcatttgtg tatgtatttt ctgtctcaaa agctatatac tgaacatgaa acttgtttga
     4501 aagagggaat tgcacgatga tttattcgaa agaaatcgtc agagagtggc tggatgaagt
     4561 agcagagcgg gccaaggacc atccagagtg ggttgatgtc tttgagcgtt gctacaccga
     4621 taccttggac aatacggttg aaatcttaga agatggttca acttttgtct tgactgggga
     4681 tattcctgct atgtggcttc gggattcgac agcccaactc agaccctacc tgcatgtggc
     4741 taaaagagat tccctcctgc gtcagaccat tgcaggtttg gtcaaacgtc agatgacctt
     4801 ggtactcaag gatccttatg ccaattcctt caacatagag gaaaactgga aggggcacca
     4861 cgagaccgac cacaccgacc ttaatggctg gatttgggaa cgcaagtatg aggtggactc
     4921 gctttgctat cctttgcagt tggcttatct cctctggaaa gagactggcg agactggtca
     4981 gtttgatgag acttttgtcg cagcgactaa ggaaattctc catctatgga cagttgaaca
     5041 agaccacaag aactctcctt atcgttttgt ccgagatacg gaccgtaagg aagacacctt
     5101 ggtaaatgat ggctttggac ctgactttgc agtgacaggt atgacttggt cagcctttcg
     5161 tccgagtgat gactgttgcc agtatagtta cttgattccg tcaaatatgt ttgctgtagt
     5221 agtcttgggt tatgtgcaag aaatcttcgc agcattaaac ctagctgata gccagagtgt
     5281 tattgctgat accaagcgtc ttcaggatga aatccaagaa ggaatcgaaa actacgctta
     5341 caccaccaac agcaagggcg aaaagattta cgcttttgaa gtggatggcc taggaaatgc
     5401 cagcatcatg gatgatccaa atgtaccaag tctactagct gcgccctatc tgggctactg
     5461 ttcggtcgat gatgaagtgt atcaagctac tcgtcgtacc attttgagct ctgaaaatcc
     5521 atacttctac caaggagaat acgctagcgg tctcggaagt tctcatacct tctatcgcta
     5581 tatctggcca atcgcccttt ctatccaagg attgacaaca agagataagg cagagaaaaa
     5641 attcttgctg gatcagctgg ttgcctgcga tggtggtaca ggtgtcatgc acgaaagctt
     5701 ccacgtagat gatccgaccc tctactctcg tgaatggttc tcctgggcta acatgatgtt
     5761 ctgtgagttg gtcttggatt acttggatat tcgctaaggg gctcgcttta gctcaaccga
     5821 ttcttatcag aatcacaagt ttacatttaa aacgttaaaa tttaaattta gaatgaggtt
     5881 ttacttcatg gaaaatgttg ttgtacatat tatctcacat agtcactggg accgtgagtg
     5941 gtacttgcct tttgaaagcc atcgtatgca gttggtggaa ttgtttgaca atctctttga
     6001 tctctttgaa aatgaccctg agttcaagag tttccacttg gatggacaaa ctattgtcct
     6061 tgacgactac ttacaaattc gccctgaaaa tcgtgacaag gtccaacgct acattgacga
     6121 gggcaaactt aaaattggtc ccttttacat cttgcaggat gactacttga tctccagtga
     6181 agccaatgtc cgcaatacct tgattggtca acaagaagct gccaaatggg gtaaatcaac
     6241 ccagattggc tactttccag atacctttgg aaatatggga caagcgcctc aaattcttca
     6301 aaaatcaggc attcacgtgg cggcctttgg tcgtggtgtg aagccgattg gatttgacaa
     6361 ccaagtcctt gaagatgagc agtttacatc ccagttttca gaaatgtact ggcagggtgt
     6421 ggatggtagt cgtgttttag gtattctctt tgccaactgg tacagtaatg ggaatgaaat
     6481 cccagttgac aaagatgagg ccttgacctt ctggaaacaa aaattgtcag atgtgcgtgc
     6541 ctacgcttcg accaaccaat ggttgatgat gaacggctgt gaccaccagc ctgtacagaa
     6601 aaatctgagc gaagccattc gtgtggcaaa tgaactcttc ccggatgtaa tctttgttca
     6661 tagttctttt gatgaatatg ttcaagctgt agaaggtgcg cttcctgaac ccttatcaac
     6721 tgttacaggt gagttgacca gtcaggaaac agatggctgg tacacacttg ccaacacttc
     6781 ttcatcccgc atttacctaa aacaagcctt ccaagaaaat agcaacctcc tagagcaagt
     6841 ggtagaaccc ttgactatta tcactggtgg acacaaccac aaggaccagt tgacctatgc
     6901 ttggaaaaca cttttgcaga atgcgccaca tgatagtatc tgtggctgta gcgtggacga
     6961 agttcaccgc gagatggaaa cgcgttttgc caaggtcaac caagtaggaa actttgttaa
     7021 aagtaatttg ctcaacgagt ggaagggtaa aattgctacg gataaggctc aaagtgacta
     7081 tctctttact gtcattaaca caggcttgca tgataaggtc gatactgtca gcacagtgat
     7141 tgatgtggcg acttgtgatt tcaaggaatt gcacccaaca gaaggctaca aaaagatggc
     7201 tgctcttatc ttgccaagtt accgtgtgga ggacttggat ggtcgtcctg tagaggctac
     7261 aatcgaagac ctcggagcta attttgaata cgatttacca aaagacaagt tccgccaagc
     7321 tcgtattgct cgacaagtgc gcgtgaccat tccagttcac ctagcaccgc tttcttggac
     7381 aaccttccaa ttgctggaag gagaacaaga acaccgtgag ggtatttacc aaaacggagt
     7441 gattgataca ccattcgtaa cggtgagtat ggatgacaac atcacagtct atgataagac
     7501 aactcacgaa gcctatgaag actttatccg ctttgaagac cgtggggaca tcggaaatga
     7561 gtatatctat ttccaaccaa aaggaacaga gccaatcttt gcagagctta aggaccacga
     7621 ggtcttggaa aacacagatc gctatgctaa aatcttgctc aaacatgaat tgaccgtgcc
     7681 tgtcagtgcg gatgaaaagc tagaagaaga gcaacaaggt atcatcgagt ttatgaagcg
     7741 tgaggctgga cggtcagaag aattgacaaa cattcctctt gaaactgagt tgactgtctt
     7801 cgttgacaat ccacaaatcc gcttcaagac tcgctttact aacactgcca aggatcaccg
     7861 tatccgtctc ttggtcaaga ctcataacac gcgtccaagc aatgattctg aaagtatcta
     7921 tgaggtggtg acacgaccaa acaaaccagc cgcttcatgg gaaaaccctg aaaatcctca
     7981 acaccaacaa gcttttgtca gtctgtatga cgatgaaaaa ggggtgactg tatccaacaa
     8041 gggattgaat gaatacgaaa tccttgggga taacaccatt gccgtgacca ttttgcgtgc
     8101 atcaggtgag ctaggtgact ggggctactt cccaacgcca gaagcacaat gcttgcgtga
     8161 gtttgaagtc gagtttgcac ttgaatgcca ccaagcccaa gaacgcttct cagcctatcg
     8221 tcgtgccaaa gccttgcaga caccgtttac cagccttcag cttgctagac aggaaggaag
     8281 cgtggttgcg actggtagcc tcttgagcca ttctgttctc agcataccgc aagtttgtcc
     8341 aacagccttt aaagtggcgg aaaatgaaga aggctatgtc ctccgttact ataatatgag
     8401 tcaagaaaat gtgcgcatat cagaacatca acaaaccatt cttgacttac ttgagcgacc
     8461 atatccagtt cattcaggac tattggctcc acaagagatt cgtacagaat tcatcaaaaa
     8521 agaagaaatt taatttcaaa aagtaaacat caaaagaaag gaggggcgaa aaggtaagaa
     8581 ctaactgctg attcgctcct ttttatggta aaaacaatga ccattgcaac gattgatatc
     8641 ggagggactg ggattaagtt tgccagtctg actcctgatg ggaaaatact ggataagaca
     8701 agtatttcaa cgcctgaaaa cttggaggat ttactagcgt ggctagatca acgcttgtca
     8761 gaacaggatt acagtgggat tgctatgagc gttccaggtg cggtcaatca agagacaggt
     8821 gtgattgatg gcttcagtgc ggtgccctac atccatggct tttcttggta tgaggcgctt
     8881 agctcttatc agctacctgt ccatttagaa aatgatgcca actgcgttgg actcagtgaa
     8941 ctactagctc attcagagct tgaaaatgca gcctgtgtcg tgattgggac agggattggc
     9001 ggagccatga ttatcaatgg tagacttcat cgaggtcgcc acggcttggg tggagaattt
     9061 ggctacatga caacccttgc ccctgctgaa aaacttaata actggtcgca actagcgtca
     9121 actgggaata tggtacgata cgtgattgaa aaatctggtc atactgattg ggacggtcgc
     9181 aagatttacc aagaggccgc agctggtaat gctctttgtc aagaagccat tgagcgcatg
     9241 aaccgtaatc tggcgcaagg cttgctcaat atccagtatc tcatcgatcc agatgtcatc
     9301 agtctgggtg gctctatcag tcaaaatcca gattttatcc aagatgtcaa gaaggctgtt
     9361 gataactttg tcgataccta cgaagaatac acggtcgcac cagttatcca ggcctgcacc
     9421 tatcacgcag atgccaatct ctacggtgct cttgtcaact ggctacagga ggaaaagcaa
     9481 tggtaagatt tacaggactt agtcccaaac aaacgcaagc tattgaggtt ttaaaaggtc
     9541 acatttctct accagatgtg gaagtggctg tcactcagtc tgaccaagcc tctatctcta
     9601 tcgagggtga ggaaggtcac tatcaattga cctaccgcaa acctcaccaa ctctatcgtg
     9661 ccttgtcctt gttggtaaca gttctagcag aagctgataa agtagagatt gaggaacaag
     9721 cagcttacga agatttggct tacatggttg actgttctcg aaatgcggtg ctgaatgtgg
     9781 cttctgccaa gcagatgatt gaggtcttgg ctctcatggg ctactcaacc tttgagcttt
     9841 acatggaaga cacttaccag attgaagggc agccttactt tggctatttc cgtggagctt
     9901 attcagcaga ggagttgcag gaaatcgaag cctatgctca acagtttgac atgacctttg
     9961 taccatgcat ccagaccttg gcccacttgt cggcctttgt caaatggggt gtcaaggaag
    10021 tgcaggagct ccgtgatgta gaggacattc ttctcattgg cgaagaaaag gtttatgacc
    10081 tgattgatgg catgtttgcc acgttgtcta aactgaagac tcgcaaggtc aatatcggga
    10141 tggacgaagc ccacttggtt ggtttgggac gctacctgat tttgaacggt gttgtggatc
    10201 gtagtctcct catgtgccaa cacttggagc gcgtgctgga tattgctgac aaatatggtt
    10261 tccactgcca gatgtggagt gatatgttct tcaaactcat gtcagcggat ggccagtacg
    10321 accgtgatgt ggaaattcca gaggaaactc gtgtctacct agaccgtctc aaagaccgtg
    10381 tgactctggt ttactgggat tattatcagg atagcgagga aaaatacaac cgtaatttcc
    10441 gcaatcatca caagattagc catgaccttg catttgcagg gggagcttgg aagtggattg
    10501 gctttacacc tcacaaccat tttagccgtc tagtggctat cgaggctaat aaagcctgcc
    10561 gtgccaatca gattaaagaa gtcatcgtaa cgggttgggg agacaatggg ggtgaaactg
    10621 cccagttctc tatcctacca agcttgcaaa tctgggcaga actcagctat cgccatgacc
    10681 tagatagttt gtctgcgcac ttcaagacca atactggtct aacggttgag gattttatgc
    10741 agattgacct tgccaacctc ttaccagacc taccaggcaa tctcagtggc atcaatccca
    10801 accgatatgt cttttatcag gatgttctct gtccgatact tgaccgacac atgacacctg
    10861 aacaggacaa accgcacttc gctcaggctg ctgagacgct tgctaacatt aaagaaaaag
    10921 ctggaaacta tgcttatctc tttgaaactc aggcccagtt gaatgctatt ttaagtagca
    10981 aagtagatgt gggacgacgc attcgtcagg cctaccaagc ggatgataaa gaaagtttac
    11041 aacaaatcgc cagacaagaa ttaccagaac ttagaagcca aattgaagac ttccatgccc
    11101 tctttagtca ccaatggctg aaagaaaaca aggtctttgg tttggataca gttgacatcc
    11161 gtatgggcgg actcttgcaa cgcatcaaac gagcagaaag ccgtatcgag gtttatctgg
    11221 ctggccagct tgaccgcatc gacgagctgg aagttgaaat cctaccattt actgacttct
    11281 acgcagacaa ggatttcgca gcaactacag ctaaccagtg gcataccatt gcgacagctt
    11341 cgacgattta tacgacttaa tactcttcga aaatctcttc aaaccacgtc agcttccatc
    11401 tacaacctca aaacagtgtt ttgagcagcc tgcagctagc ttcctagttt gctctttgat
    11461 tttcattgag tataaaaaca agaacacctt gcttggcgca gggtgtttcg cgtgaaacag
    11521 aagaattatc tggtttcaaa tgctacagtt aaaattagta aaacaagaaa tagaagctga
    11581 aaagccagca tctgtagagg cttggatcat ttccgtcaaa ttaaaaaagg ttgctaccga
    11641 catatataga ttccaaaaac aaaaacgtta gcggagctag cagatgtgat tttatggagt
    11701 tttgaatgcc aagtgctgag agaaatcgag acagaggacg aagaagctta tctcgtacgt
    11761 tcggttggaa cgtcgccaga acaatatcca gattatgatg gttttgacta tgaagaatgg
    11821 taaaattgaa atcagtctgt gtgtaggctt agtatttcaa taggttgttg aaaatatttt
    11881 aaacgttttt actttggcaa agatgttctc aaccttgctt ctctccttag atagcgcatg
    11941 gttataggct ttatcttcag ctattagcgg cttgagtttg ctggatttac gtggagtttg
    12001 tgcttgagga tatatcttca tgggcccttg ataaccactg tcagccaaga ttttaccagc
    12061 ttgtccgata tttctgcgac tcattttgaa caacttcata tcatgacaat agttcacagt
    12121 gatatccaaa gaaacaattc tcccttgact tgtgacaatc gcttgagcct tcatagcgtg
    12181 gcatttcttt ttaccagaat cattcgctaa ttcttttttt agggcgattg atttgtactt
    12241 ccgtcgcatc aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaacac
    12301 cactttgaac aagagttact tcaacccatt ggctccgacg gataaagttg ctttcgtgga
    12361 taccaaaaac agccgcaatt tcttcataag tgcggtattc tcgcacatat tgaagagtgg
    12421 ccataagaag gtcttctaga cttaatttag gttttcgtcc accttttgcg tgtttaagtt
    12481 gataagctgt ttttaataca gctaacatct ctttaaaagt ggtacgctga acaccaacaa
    12541 gacgcttaaa tcgtgcatca gttaattgtt tacttgcttc ataatttcgc agggagtcta
    12601 ttgactcttt ggtaggtgtc aatgtttttt tcatctatcc cgagaattat tttcccgcca
    12661 tttgtatttg caaatgctga gtaggtttcc cagaaagact ctggaagatt gtttttagct
    12721 tttttgtatt ctaaatcaac cccttcaaat tttaagtcca tatttttcct ttacatctgt
    12781 tttttgtggt tctggtattt gttcaagttg agtgataata tagcgaattg aatttcgaga
    12841 gtttttactc agttaatttc ttttttaaca cgtgttaaaa aataaattaa agcgggttaa
    12901 tttttcttga cttaaaattt aaaaatgata tactgtgttt atggagagat gacaatttat
    12961 aaaatgtact actctattct actagataat gattgaataa aactttttat aacaacggct
    13021 agcaaagtta taattttata tctattagaa aataaatatg taaggaaatt taaatgaaaa
    13081 aaactacaat attatcatta actacagctg cagttatttt agcagcatat gtccctaatg
    13141 aaccaatcct agcagatact cctagttcgg aagtaatcaa agagactaaa gttggaagta
    13201 ttattcaaca aaataatatc aaatataagg ttctaactgt agaaggtaac ataggaactg
    13261 ttcaagtggg taatggagtt actcctgtag agtttgaagc tggtcaagat ggaaaaccat
    13321 tcacgattcc tacaaaaatc acagtaggtg ataaagtatt taccgttact gaagtagcta
    13381 gtcaagcttt tagttattat ccagatgaaa caggtagaat tgtctactat cctagctcta
    13441 ttactatccc atcaagcata aaaaaaatac aaaaaaaagg cttccatgga agtaaagcta
    13501 aaactattat ttttgacaaa ggtagtcagc tggagaaaat tgaagataga gcttttgatt
    13561 tttctgaatt agaagagatt gaattgcctg catctctaga atatattgga acaagtgcat
    13621 tttcttttag tcaaaaattg aaaaagctaa ccttttcctc aagttcaaaa ttagaattaa
    13681 tatcacatga ggcttttgct aatttatcaa atttagagaa actaacatta ccaaaatcgg
    13741 ttaaaacatt aggaagtaat ctatttagac tcactactag cttaaaacat gttgatgttg
    13801 aagaaggaaa tgaatcgttt gcctcagttg atggtgtttt gttttcaaaa gataaaaccc
    13861 aattaattta ttatccaagt caaaaaaatg acgaaagtta taaaacgcct aaggagacaa
    13921 aagaacttgc atcatattcg tttaataaaa attcttactt gaaaaaactc gaattgaatg
    13981 aaggtttaga aaaaattggt acttttgcat ttgcagatgc gattaaactt gaagaaatta
    14041 gcttaccaaa tagtttagaa actattgaac gtttagcctt ttacggtaat ttagaattaa
    14101 aagaacttat attaccagat aatgttaaaa attttggtaa acacgttatg aacggtttac
    14161 caaaattaaa aagtttaaca attggtaata atatcaactc attgccgtcc ttcttcctaa
    14221 gtggcgtctt agattcatta aaggaaattc atattaagaa taaaagtaca gagttttctg
    14281 tgaaaaaaga tacatttgca attcctgaaa ctgttaagtt ctatgtaaca tcagaacata
    14341 taaaagatgt tcttaaatca aatttatcta ctagtaatga tatcattgtt gaaaaagtag
    14401 ataatataaa acaagaaact gatgtagcta aacctaaaaa gaattctaat cggggagtag
    14461 ttggttgggt taaagacaaa ggtttatggt attacttaaa cgaatcaggt tcaatggcta
    14521 ctggttgggt taaagacaaa ggtttatggt attacttaaa cgaatcaggt tcaatggcta
    14581 ctggttgggt taaagacaaa ggcttatggt attacttaaa tgaatcaggt tcaatggcta
    14641 ctggttgggt taaagacaaa ggtttatggt attacttaaa cgaatcaggt tcaatggcta
    14701 ctggttgggt taaagacaaa ggcttatggt attacttaaa cgaatcaggt tcaatggcta
    14761 ctggttgggt taaagacaaa ggcttatggt attacttaga tgaatcaggt tcaatggcta
    14821 ctggttggtt tacagtttct ggtcaatggt actataccta taattcagga gatttattag
    14881 taaacacgac tacacccgat ggctatcgag tcaatgctaa cggtgagtgg gtaggatagg
    14941 tatccattag ctaaatctaa tcattctgaa ctaaaaaatc ttcttacata tgtaggaaga
    15001 ttttttggat tctcaaaact agtttaaaag actacgctat ttaaaccaat tttaagaatg
    15061 gatatataag gatattcaga aatctagttg gtcaactttt gtattttgaa tataccttag
    15121 atggtgacga tgtcttggta tatctggtac tcacttacat attctaactt attttaacta
    15181 agatacaact gtagttggtg aatttgctac aaatagtgta gaggcgttat ttgaaaaata
    15241 tacaaaaaag gaaccccaac ttacatttga gttcctcttg cttttaattg tccgaatccg
    15301 tattcttatt tcaataatgt agtcattcga atgatttccc ttgcaatata gtgaaattaa
    15361 caaaaagaaa aaacgctgat caagcgtttt tcttctgtat tgattcgtat tgaatgctta
    15421 cttcacttct gtaaacacaa cgtgtttgcg aagttttggt gagtatttct tcaattgaag
    15481 gcggtctgga gtgttacgtt tgtttttaga agtaaggtac aagcgttcac cagattcttt
    15541 gtgttcaagt gtaatattta cgcgcatagt atctcccttc tattattcag ctgatgcagc
    15601 tttagcgatt ttacgtcctt tgtagtatcc tttaagtgat acacggtgag aacgtgagta
    15661 atctccagta gtttcgtcaa agtttacaga tggagctgtt actttgtagt gtgtacgacg
    15721 tttgtttttc ttcgcttttg aagtgcgacg tgcaggtact gccattttga tttctccttt
    15781 aggtatttat attcgattca atctactgat tttcatcaac catactagga taacacattt
    15841 tttttgtaaa gtaaagttac ttgacaaaaa aagatgaaaa aattctccta tccgacacgg
    15901 cagacaggag aaggtgttaa atatcaatct caaatggttc gtcaatggtt tctgatacgt
    15961 attttccgtc ttttttccgt tgcttgacac actctgtgag gagatattct atttgcccat
    16021 tgactgaacg aaagtcgtct tctgcccatg atgcgagtgc agcgtataac tttgttgaga
    16081 gtcgaagggg gatctgcttt tttttagctt cagccatctt tagtaaagac ttcctgtgtt
    16141 gacaattggt tgtgcatcat gattgccaca aagaacgaca aggagatttg aaaccatggc
    16201 agcttttcgt tcttcgtcaa gttctaccaa ttccccttca ttgagccgtt ctagtgccat
    16261 ttcaaccatt cctacagcac catctacaat catcttacgt gcatcaataa tggcagatgc
    16321 ttgttggcgt tgaagcataa cggcagcaat ttctggagca taagctaggt aagtgatacg
    16381 tgcttcaagg atttccaagc cagcatcctc aacacgactt tggatttctt cacgaatacg
    16441 gttagcaaca atttcgctag agccacggag actaccttca tctgcttgcc cgtcacccgt
    16501 agtatccaca ttaggagaca catcgtaagg atagatgcgg acaatattac ggagggcgct
    16561 atcacattgc aatgaaagat attctttgta gttatcaacg ttgaagactg ccttagcggt
    16621 atcgacaact ctccaagtta ccgcgatacc gatttctaca gggtttccta agcaatcatt
    16681 gattttttga cgagaattgc tcaaggtcat gactttgagg gaaatctgtt tcttgccaat
    16741 ttcaagattt acatcattgt catttgatga tttagctcct aaaaaaggag attttgtgct
    16801 aacatcacca ctttgtccaa gtcgagtgtg gtttgcaggg ttgactgcta cgctgaaggg
    16861 attgacaaag taaaaaccag gttctttgat ggtacctgta taattaccaa agagtgtcag
    16921 aaccagagct tcctgaggtt tgacaacttt taaaccagca tgagctagac ctgcaattac
    16981 gattagtaaa ggtccgataa taattccaaa aatgttttcc gaaccaacac ccattataaa
    17041 tataaagata ccaagtacga ttgtcaattc aatgagaatc aatgcgaata caccatttgg
    17101 ttttgaattg atgagttttt cagtcataag aatgacctcc tattatttga tatctaaatg
    17161 atatcacttt gtttttaaaa gtcaatagaa agttgaaaat aatcactaaa aagtttcagc
    17221 ccaataattt cttttaaaag gtgagattat attgtgctca aatactcttt tattcttata
    17281 atttttacac tataattgta gttagaaagg aagagaaatg tttgattata gagcactagt
    17341 tattttgatg actttgatgg atcattgtga gctatcatta tatgaattat ctgttaaggt
    17401 gagcttacct ataaaggaag tcaaagaagg aatagattat ttagtgcctt atctagctaa
    17461 taaagggata gtgctggata aaaaacaagg tcgctatagt ttatcaaatc gtacgaagca
    17521 gtctttgaca gatattatta agtcggatga attggtttta ccaaagtcga ctcgcttagc
    17581 tttaatctat ctttacactt tttgccgatt agattttata tcgaataatc attaccaaga
    17641 ctttttgaaa gtaagtaaga atacaacctt atctgatatt caatcattaa gaaagattat
    17701 gttagataat gatttggagc tagggtacag tagagcaaaa ggttatactt tacacggttc
    17761 agagtggaat aagcaccgct tagcttttca aatggttagt gagttgctgg aatcctccat
    17821 agggatttgg ggtttggatt atgttttatc cagttggggg tattcattaa cttatgattt
    17881 gataaatcag gtagttaaag attattatga gaagctacag ctagtaccta ttgttaatca
    17941 attaaaagtt tgccttttcg gtttagtatt cattctgtgt aggtatcaaa gggatgttga
    18001 aagagtatgt ctttcagaga cattagtatc tcctgttatt caagatataa cgactatttt
    18061 attggataca gtggttgatt tgggaattat agatacggtg ttttctgagg atgattatcg
    18121 ctatatcacc gttttattat caagttgctt tgaaggtgaa gtagatgttg ctccggttta
    18181 ctttaatcaa ttaacagagg ctattataag tagaatggaa gatatttctt tgttacattt
    18241 taaacaaagg gaaggattga gagaaaatct tcgccgccac cttataccgg cttactatag
    18301 attaaagttc ggcctaccta gttcaaatga atatgtattg catgttaaag aacattatcc
    18361 tgatttattt gaattagtca aagattcttt gatgcccttg atggacgcta ttgacaatcc
    18421 catacctgat agtgaaacag catattttgt tatccatttc ggaggttatt taaagaaagc
    18481 agatactttg cctcaaaaat gctataaagc agcaatcatt tgtcctaatg gtgttagttc
    18541 ttcattgatg ttaaaagaga atctattagc attatttcct caaattgagt ttataggaac
    18601 ctcaaagatt gatgatttac aggtgaaagc tagtagtgac tatgatatgg ttttttctac
    18661 cataaaggtg gagacagaaa agccaaatta tctagtttcg gttatgatga ctgaagagca
    18721 agcaatacaa ctagtagaac tagtgttaaa agattttccg aatttagagt atggggattt
    18781 tgagattgaa caaatcctga atattgtcaa aagatatgga attattacac aagaattgga
    18841 attaagattg gcgttaaaga attatcttta ccaaaaaaat gatagaaagg aaattgtacc
    18901 agtgctagaa caactcatta ccaaagaaac ctatcaggtt agttcgcaaa aattaggatg
    18961 gaaggaggcg attcgtttag cagctaaacc gcttttggat caagataaga taactgagaa
    19021 ctaccctgag gcaatgattc aaaaagtaga agagtttggg ccttttatta atttagggaa
    19081 gggagtagca attcctcacg ctcggccaga tgaaggtgtg aatgaaatag gaatgtcaat
    19141 gttagttttg gaagaaccga tttatttatt ggataatcca gaacaagagg taagattgtt
    19201 gatttgtatt gcagccattg ataatgagag tcatttaaag gctttgtcac atttaacaac
    19261 aattttaaga gataaaaatc atgttcaaac tttaatatca tcaaaaaact atgatgacat
    19321 taaaatgata attaaacagg aggattagat aatgttaaaa attggtacag cttgtggttc
    19381 aggattaggt tcaagtttta tggtacagat gaatattgaa tctgtattga gtgatttgaa
    19441 tgtttcggat gtagaagttg aacattatga tttaggtgga gcagatccaa atgcagctga
    19501 tatttggatt gttggtcgtg atctagctga ttcagctagt catcttggag atgttcgtat
    19561 cttaaatagt attattgata tggatgaact acgagaatta attactaaac tttgtgaaga
    19621 aaaaggactt atataggagg ctagcgttat gatgaagttc atattggata ttgttagtac
    19681 accagctatt ttagtagctt taattgcaat tttaggatta gttcttcaga agaagaaatt
    19741 acctgatatt attaaaggtg gaattaagac ctttgttggt ttcttagttg tatctggtgg
    19801 tgcaggaatt gtacaaaatt ctttaaatcc atttggtacc atgtttgagc atgcttttca
    19861 tttatctggc gttgtgccga ataatgaagc aattgtagct gtagctttaa caacatatgg
    19921 ctcagctact gcactgatta tgtttgcagg catggtgttc aatatcttaa tcgctcgttt
    19981 tactcgattt aaatatattt ttttaacagg gcaccacact ctatatatgg catgtatgat
    20041 tgcggtcatt ttatcagttg ctggctttac tagcttgcct cttatcttac taggaggatt
    20101 agcactcggt attattatga gtatttcccc agcatttgtt caaaaatata tggttcaatt
    20161 aactggaaat gacaaggtgg ctttaggtca tttcagttct ttgggatatt ggttgagtgg
    20221 ttttactggt agccttatcg gtgacaaatc aaaatcaaca gaggacatta aatttccaaa
    20281 gagtttagct tttttacgtg atagtactgt tagtatcact ttatccatgg cagttattta
    20341 cattattgta gctatctttg cagggtcaga atatatagaa aaagaaatca gtagtggtac
    20401 aagtggtcta gtttatgctt tacaattagc aggtcaattt gcagcagggg tatttgttat
    20461 tttagcaggt gttcgcctta ttttgggcga aattgttcca gcctttaaag gtatttcaga
    20521 gcgtcttgta cctaattcaa aacctgcttt ggattgtccg attgtttata cttatgcacc
    20581 caatgcagtt ctaattggat ttatctctag ttttgttggt ggtttagtaa gtatggcaat
    20641 tatgattgct tcaggaacgg ttgttatctt accaggtgtt gtgcctcatt tcttctgtgg
    20701 agcgactgca ggtgtcattg ggaatgcatc tggtggtgtt cgtggagcca ctattggagc
    20761 atttttacaa ggtattttaa tcagttttct tccagtcttt ttaatgccag ttttgggagg
    20821 acttggtttc caaggatcaa ctttctcaga tgcagatttt ggtctatcag gaattatttt
    20881 aggaatgtta aatcaatttg gctcacaagc aggcattgtg attggtcttg ttcttattct
    20941 agcagttatg tttggagtat cctttattac aaagccatct gcaaaggagg aataagggat
    21001 gattttaagt aaaaataggg aagatgagtt aagaaaattt gcgactaaca tccgattaaa
    21061 tactcttaga acattgaatc atcttggatt cggacattac ggaggaagtc tgtctatcgt
    21121 agaagtttta gcagtgcttt atggtgaaat aatgccaatg actccagaaa tatttgcagc
    21181 acgagataga gattatttca tattatcaaa aggtcacgga ggaccagctc tatacagtac
    21241 actctatttg aatggtttct ttgacaaaga attcttatat tctttaaata caaatggaac
    21301 caaattaccg tctcatcctg atagaaatct aacgccaggc atagatatga caacgggctc
    21361 tttaggacaa ggaattagtg ttgcaactgg acttgcatat ggtcagagaa taagaaagag
    21421 tcccttttat acttatgcta ttgttggaga tggtgagtta aatgagggac aatgttggga
    21481 ggctatacag tttgcttctc atcaacagtt atccaactta attgtatttg ttgatgataa
    21541 caaaaaacaa ttagatggtt ttacaaagga tatttgtaat ccaggtgatt tcgtagaaaa
    21601 attttcagca tttggatttg aatccattag gatcaagggt tcagatatta gagaaattta
    21661 tgaagggatt gtccaattaa aacagtcaaa taattcatca cctaagtgca ttgtattaga
    21721 tactattaaa ggtcaagggg ttcaagagct ggaagaaatg aaatccaatc atcatcttcg
    21781 ccctactgta gaggagagac aaatgttaac ttcagttgta gaaagattaa gtcaggaatt
    21841 ggaggaaaca gaatgatgag aagtacgaaa gaattacggc atgtatatag agacttcctt
    21901 ttagaggcta atcaaagtta ttctgatata gtagtcttag aagccgatct gtcaagttcg
    21961 atggctactc ataatcttga aaaggacttt ggagaccgtt atgtgaatgt tgggattatg
    22021 gaagcagaaa tggtcggcct tgcagcaggc ttgtctattc aggggtttag accttatctt
    22081 catacatttg gtccttttgc ttcacgaaga gtatttgatc aattatttat ttctcttgga
    22141 tacgcacaat tggatgccac tgtgattgga tcagatgcag gagtaactgc agaaatgaat
    22201 ggtgggacac atatgccatt tgaagaaatt ggattgttac gtttaattcc taaatcgatc
    22261 atttttgaag caactgatga tatccaattt cgtgaaatat tgaaccagac attagactta
    22321 aaaggactaa aatatattcg aacaattaga aaagctccag ttcctgtgta tcaaggtgga
    22381 gaagattttt ctaaaggcta cattgagtta aggcatggtg aagatcttgt aatcgttgct
    22441 tctggtataa tggttgctcc aagtattcga gttgcagatg aactgtctaa attaggttat
    22501 tcagtaggtg tgatagattt atttagaatc aaaccgatac cagaacagat aaaaacaatg
    22561 ttaagtggaa aaactatatt tactgtagaa aaccacaatc agataggtgg aattggcagt
    22621 gctttatgtg aattattctc tgatgatgga ataacaaaaa ttcatcggat gggtgttcaa
    22681 gaaagatttg gtcaagtagg gaaaatggat tatcttctta atgagtatgg tttgagtgaa
    22741 tcgaatataa aagagaaggt tctagaaatt tataatgcaa gatagatgtt aaatacactt
    22801 atctgaaaga atttctgtaa aaattacatt acgttattta aagaaagcgg attaaaaagc
    22861 atattctggt catcagaaag tgcactaatc cgctttgttg tttttaattt taaatatttc
    22921 acatgcgtat agaattttct gaaaattcaa gaattttatt ctgttcattg cctttaaatt
    22981 gtgctataat agtaaaaact gaaatgggag ggataagatg actgaattag ataaacgtca
    23041 ccgcagtagc atctatgaca gcatggttaa atcacctaac cgtgctatgc ttcgtgcgac
    23101 tggtatgaca gataaggact ttgaaacatc gattgtggga gtgatttcga cttgggcgga
    23161 aaatacacca tgtaacattc acttgcatga tttcgggaaa ctggctaaag aaggtgtcaa
    23221 atctgcaggc gcttggcctg tacagtttgg aaccattacc gtagcggacg ggatcgctat
    23281 gggaacgcct ggtatgcgtt tctctctaac atctcgtgac atcatcgcgg actccatcga
    23341 ggcggctatg agtggtcaca acgtggatgc cttcgtcgct atcggtggct gtgacaagaa
    23401 catgcctgga tctatgattg ctattgctaa tatggatatc ccagctattt tcgcctatgg
    23461 tggaactatt gcaccgggaa atcttgatgg taaagatatc gacttggttt ctgtctttga
    23521 aggtatcgga aaatggaacc acggtgacat gacagctgag gacgtgaaac gtcttgaatg
    23581 taatgcctgc cctggccctg gtggttgtgg tggtatgtat actgctaata ccatggcaac
    23641 tgctatcgaa gttctaggga tgagtttgcc agggtcatcc tctcacccag ctgaatcagc
    23701 tgataagaaa gaagatatcg aagcagcagg acgtgctgtt gttaagatgt tggaacttgg
    23761 tctcaaacca tcagatatct tgactcgtga agcctttgaa gatgctatta ctgtaacgat
    23821 ggctctcggt ggttctacaa acgccactct tcacttgctc gccattgccc atgccgcaaa
    23881 tgttgacttg tcacttgagg acttcaatac gattcaagaa cgtgtgcctc acttggccga
    23941 cttgaaacca tctggtcagt atgtcttcca agacctctac gaagtcggtg gtgtccctgc
    24001 ggttatgaag tacttgttgg caaatggttt ccttcacgga gatcgcatca catgtactgg
    24061 taagactgta gctgaaaact tggctgactt tgcagacttg actccaggcc aaaaagttat
    24121 catgccactt gaaaatccaa aacgtgcgga tggtccgctt atcatcttga acgggaacct
    24181 tgctcctgac ggtgcagttg ccaaggtatc aggtgttaaa gtgcgtcgtc acgttgggcc
    24241 agctaaggtc tttgactcag aagaagatgc gattcaggcc gttctgacag atgaaatcgt
    24301 tgatggcgat gtagtcgttg ttcgttttgt tggacctaaa ggtggtcctg gtatgcctga
    24361 gatgctgtca ctttcttcaa tgattgttgg taaaggtcag ggagataagg tggccctctt
    24421 gacggacgga cgtttctctg gtggtactta tggtctggtt gttggacata tcgctcctga
    24481 agctcaggat ggtggaccaa ttgcctatct ccgtaccggc gatatcgtta cggttgacca
    24541 agataccaaa gaaatttcca tggccgtatc cgaagaagaa cttgaaaaac gcaaggcaga
    24601 aacaaccttg ccaccacttt acagccgtgg tgtcctcggt aaatatgccc acatcgtatc
    24661 atctgcttca cgcggagccg tgacagactt ctggaatatg gacaagtcag gtaaaaaata
    24721 aactcatact cgaaaatctc ttcaaatcac gtcaacgtcg ccttgccgta ggtatggtta
    24781 ctgacttcgt cagttctatc cacaacttca aaacagtgtt ttgagcaacc tgcggctagt
    24841 tttctagttt gccctttgat tttcattgag tatcaaaaag caagccattt tcggcttgct
    24901 tttttcatct ttaaaagtga ttggcctgct taacgaggtg gtagtccaag ggcaatacgg
    24961 ccatagcgac tgattcgtgt gattttccaa gcaggcgacc aggtaacttc aaccttgata
    25021 tctttgatac cctcgatttg tttcagacct gccacgattt caataggcag gctttcggcg
    25081 caatcacagg cagtatcggt gaaggtcatg acaatcttgc agagccccgt ttcatccaga
    25141 ttgatttcat aaatcagacc tagattgtaa acatccaatt ccacatctgt atcaaaaact
    25201 ttctctagtt tttcgataat ttggtcttgc aaggccaaag cacggtcatt gattttgata
    25261 tcgtctctca tagcagtccc tccacgtgat aaatatcctc tattttctca taattctgac
    25321 aatttggcaa gtttttgatg aattttctga aaaatatgat aaaatgaaga aaatacggaa
    25381 tcaaggggaa gtctatggag cagattggaa aagtctttag acaattacga gagtcaagaa
    25441 atatctcact gagacaagca accagaggac aattttcgcc gtctatgttg tcccgttttg
    25501 aaacaggtca gagtgagctt tcggtggaaa agtttctatt tgctctggaa aatatatctg
    25561 ccagtgtgga ggagctcctc tttctggcga gaggtttcca gtatgataca gattctgagt
    25621 tgagaaaaga aattacagat gtcttgaaac caaagaatat agcacctctt gaggacttat
    25681 atcgcaggga gtatcaaaag catgcccatt ctcacaacaa acagaaacat attctaaatg
    25741 ccattattat caagtcttat atgaagagca tagatgaaag ggtagactta acagcagagg
    25801 aaggaaaagt ccttcatgat tatttgtttt ctaccgagat ctggggaatc tatgaactca
    25861 atttattttc agtcagttct ccatttttat ctgtttctct ttttactaga tatgtacgag
    25921 aaatggtacg taaatctgat tttctaatgg aaatgtctgg caatcgaaac ctttttcata
    25981 ctatactatt gaacggcttt ttagctagta ttgagtgtga agaatttacc aatgcctatt
    26041 attttaaacg tgttatcgaa gagcatttct acaaggaaaa tgagacctat ttccgaattg
    26101 tttatctgtg ggcagaaggt ctccttgata gcaagcaagg tagagtcaag gaaggccaga
    26161 aaaagatgga ggatgctgtc cgtatttttg agatgcttgg ctgtaataaa tctgccgaat
    26221 actatagaaa tacgaccgaa tgttgaatat ctgcaaacca agaaaaaatt tgaagcgata
    26281 gaactattgc cctctctcag gtcattacaa aagttctatc gtttcttttt gctcatttta
    26341 tttgttgcat atattcaaga gttctccctc tcaaatttct aagtgctata ctatagtcat
    26401 acttcatata gaggttcgaa caagaaggga gattatctat gaaactattg tttagaaatc
    26461 cagcttatag aattttgact ttatcacgct tcttcaatgc ttttggtgct tcgattttca
    26521 acctggtctt tatcgtctat gcatcgacct tgtcacaagc atcttttgct gttgctatgg
    26581 cgaatattgt catgattctt ccgaccctct ttacagtttt tgcagggatt cgggcagatt
    26641 acacgaggga caaggtcaaa tggatggtct atagcggttt gtttcaggcg gttctatttt
    26701 ttctagcagc cctagttgtt cagcaagcta gtctctttgc cttttctagc ctgtgtttga
    26761 tcaatgtcat tagtgatatc atcagtgatt ttgcaggtgg cctgcgtatg cctcttatta
    26821 aggaaaaagt agctgaagat gatctgatgg aggcttattc tttttcacag ttcatcacct
    26881 atatttcagc tattggtggt caagctttcg gagtctggct cttagctcta tcggtcaaca
    26941 atttttctct cgttgcggga atcaatgcct gctttttcct agtatcagcc actatcctct
    27001 ttttaggaaa aagcaaattg agcctgtcaa tgtcatctgc tgatggtgaa aacttaaaaa
    27061 atgagaagct ttctatcaaa gaccagttcc taacaattta ccgaaattta cgtctcgttt
    27121 ttcttaaaag tggacagaaa aactttggtt ttatgctctt tgctgtcttg cttattaatt
    27181 ccttgggtgg cgctttgggt ggaatttata atatcttctt tttgagccat tctctcttga
    27241 atttttctta tacagaggca ctatttatca atcaattctg tgttttagta gcagtcatca
    27301 tcagtagcct tacgggcaat gattattttg ggaagcagtc cttgcctaga ttgatgatgt
    27361 gggcgaccgt agggcttagt ctagttggtt tggctaattt attcaatcaa gtcgtgcttg
    27421 gtttgctatt tctcttcttt actctgtatg tgtctggtaa agttcaacca aagattagtg
    27481 ccatgctcat gaaaaatcta gctccagagg ttctagctcg tactagtaat tttttaggtc
    27541 tattgtttac cttatccata cctgtgggaa cagcttgttt ttcactagta gccgtatgga
    27601 atatacagtt gacttggatg ctatttgttg gtctttcctt gctagctatt ttttttgaca
    27661 attcttaatc tcaaaaatga tatctaaatc ctaatttttt tcttactgtt ttaatccctc
    27721 tatttatggt aaaataagac tattaagttt aaagaggatt ccctatgaaa ttacaaaaac
    27781 caaaaggaac gcaggatatt ttacctgctg aatctgccaa gtggcagtac gttgagggct
    27841 ttgcccgtga gattttcaag cgctataact atgcagaagt gcgcacgcct atttttgagc
    27901 attacgaggt tatcagtcgc tctgtcggag atacaacgga tatcgtaacc aaggaaatgt
    27961 acgattttta tgacaagggt gaccgccata ttaccctccg tccagaagga actgcgcccg
    28021 ttgtccgtgc ctatgtggaa aataaactct tcgccccaga agtgcaaaag ccaagcaagt
    28081 tctactatat gggacctatg ttccgttatg agcgtccaca ggcagggcgc ttgcgccaat
    28141 tccaccagat tggtgttgag tgttttggct ctagcaatcc agctaccgat gtggaaacaa
    28201 tcgttatggc agcccatttt ttgaaggaaa tcggtattca aggtgtcaaa ttgcacctca
    28261 acactcttgg aaatcctgag agccgtgcag cctaccgcca agccttgatt gactatttga
    28321 caccgctcaa ggagaccttg tctaaggata gccaacgtcg cttggaggaa aatcctcttc
    28381 gtgtcttgga ctctaaggaa aaagaagaca aggtggcagt agagaatgcg ccgtctatct
    28441 tggactttct tgatgaagaa agccaagctc attttgatgc tgtgcgtcag atgttggaaa
    28501 atcttggagt agattacatc atcgatacca atatggtgcg tggtctggac tactacaacc
    28561 acaccatttt cgagtttatc acagagattg agggcaatga cttgacagtc tgtgcgggtg
    28621 gtcgctacga tggtttggtt gcttactttg gaggccctga aactgctgga tttggttttg
    28681 ggcttggtgt agagcgcctg cttctcatcc ttgaaaaaca aggcgtggcc ctccctatcg
    28741 aaaatgccct agatgtctat atcgcagtct tgggtgatgg agcaaatgtc aaagccctag
    28801 aactagtcca agctcttcgc caacaaggtt tcaaagcaga gcgtgattac ctcaaccgta
    28861 agctcaaagc tcagttcaag tcagccgatg tctttgcggc taagaccctc atcaccctag
    28921 gagagagcga agtcgaaagc agacaagtga cagtcaagaa caaccaaacc cgagaagaag
    28981 tgcaagtgtc acttgagaca atcagccaaa acttctcaga aatctttgaa aaactaggat
    29041 tttatactca ataaaaatca aagagcaaac taggaagcta gccgcaggtt gctcaaaaca
    29101 tggttttgag gttgtagatg aaactgacga agtcagtaac catacctacg gcaaggtgaa
    29161 gctgacgtgg tttgaagaga ttttcgaaga gtattaatga tagataaagt ggtcaggaac
    29221 ttactcctga cctttttatt ttgcaaaatg acaaaaatca taaacttttt gacaactata
    29281 cttgtcaaaa agaaaaagat gtgttacaat gtaagcaagt taagagaaaa gaagaaagga
    29341 gctatgatgt gggtactagt atttatacta tttattattt tcttttattc taatgattct
    29401 aaaaaaatca agaaactaga gaagaaaatc aaaagacttg agcgaaaaga gaaaggaaac
    29461 gcagaaatgt cgagattatt acaagaaatg attggaaagg aaccaattat aacgggagtg
    29521 tatattgggc cagataactg ggaagttgtg gatgttgatg aggaatgggt caaactacgt
    29581 agtgttgata atacgggaaa agaaaaattc aagttgcaac gtattgaaga tatccaaacc
    29641 gttgaatttg acggagagta ggtgtgtaac gtgcaactaa aaaatcgtct aaaagagctt
    29701 cgagctcgcg atggtctcaa tcaaaccgac ctagccaaac tggcaggggt ttccagacag
    29761 accattagcc tactagaacg ggatgagtac accccatcca ttatcattgc cctgaaaata
    29821 tctcaaattt tcaacgaaac agtcgaatcg gtatttcgtt tggaggagga cgagtgatga
    29881 acaagtataa agtgatttat tatgtagtgg tcatagcttt attagtcagc gtatttctac
    29941 tgattgggat ggacctaagc tggtttaatc cctatcaaag cgaccaattt gtttgggtct
    30001 actttgctct catcccagta actgaatgga ttgaaaagaa atccaaaaat ctagcaagtg
    30061 aaaaaggaga atgaatatga aaaagaaacg tagattgtta tttttgatgt ctattgtctt
    30121 gggggggttc ttgggcatgt ttgtagggat gtttaaggca cgtgtcgaat cccacgaaat
    30181 tattttagat gtaaaagcct tgatgccatg gatatcagct atttgtttac tgataggttt
    30241 cattagtatg tttttgactt tcaatttctt aaagaaaagc agaaaatttc attccttgta
    30301 tcaagaggaa atggatgacg atctgaatga aacctattat gtgcaaatgt atcggaatct
    30361 tgagtttgga accattgctt ttaatattac aggtgtagcg attccattgg ctatttttat
    30421 ttcattaagt gaggtgatta tattgcatac aaaccctcaa acatttttcc tttctttctt
    30481 actctttgtg gtattcttag tcgctcaaaa atctcttttt aaaaccattg cgattgttcg
    30541 tcagtttgat ttggaatttt tcgctacacc aaaggatgtc ttgaactata taaattctta
    30601 tgatgaaggg gagcgtcagg ctaatttgga acagagtttt cgaattttat tccaattaca
    30661 ccaatatgtc ttaccagcct tatatatttt tcttattatc atttctttct tgacaggaga
    30721 gattcagtta ctagctttct tgcttgtagg agccatccat gtttatatca atgtgatgca
    30781 gttacctatg gtaaaacgtt atttcaaata aaggagttat ctatgaaatt acttaaaaac
    30841 cttggctgga ttcttctagc ccttctatcc tttttcttta tctatggctt tattcagggg
    30901 ctcgcgactg cgtcgcttgc tttaggcgct tcaccctatg ctgttactct actctatgtg
    30961 gtcttggctg gagtatatgt gtacggcatt tacaaatggt atcagaaagc tcctgtttat
    31021 attgagaaga gcggctttaa ccgatttatt tggcttcctg ccttggtttg gttcttatct
    31081 ctggtcgttc aattcttctt gccagatgat ccttcagtaa atcaacaaat agcgacagac
    31141 ttgaccttgt ctcaaccact tttctcattc tttgctgtgg ttatttttgc tcctttgacg
    31201 gaagagattg tttttagagg gatgttagca cgctatctct ttcctaagca ggacaatagt
    31261 aaacgaaccc tgatttttct tctggtatct agtcttctat ttgccttgat tcattttcca
    31321 ggtgatgtgc aacaattttt tgtctatttt agccttggtt ttagtttggg tttggcttac
    31381 attagcagaa aaggtctggt ctacagtatt tctcttcacg ctttgaataa tttagtcggc
    31441 tttttgatga ttctcatgct ataatagagt caggaggtca catgaaacga gtaattttat
    31501 tagcagtgat tcaggcagtc gttctatttt tcatcattgg agcgctagct tatgccttca
    31561 aaggcgattt cttctataac tatctagcag ttgtctttgc tcctattgca ggtgtactgc
    31621 gttttgggac ggcttacata acggaaattg tcttgcctcg aaaggcagcc gaaatcgctg
    31681 aaaagcgtaa agcaggcaaa aattcaaaat aaaagagtcc ggtgaagttc atcggatttt
    31741 tgtattatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa
    31801 acactgtttt gaggttgtgg atgaaactga cgaagtcagc tcaaaacatg gttttgaggt
    31861 tgtagatgaa actgacgaag tcagtaacca tacctacggt aaggcgacgc tgacgtggtt
    31921 tgaagagatt ttcgaagagc attagcgcta atttttaggg ctttcacctg acttttaaag
    31981 actggcaaac ttttctgatg attttcatga agagcctgcg cttttatggt aaaatagtaa
    32041 cagaataaaa gaggagagaa ataatgaaac gtagtatgta tgctggtcgt gttcgtgagg
    32101 aacacatcgg acaagaaata accttgaaag gatgggttgg ccgtcgtcgt gaccttggtg
    32161 gtttgatctt tatcgatctt cgtgaccgtg aaggaatcat gcagttggtt atcaaccctg
    32221 aaaaagtatc tgcagaggtt atggcaacag ctgaaagcct tcgtagcgaa tttgttattg
    32281 aggtgactgg tcaggtcgct gcgcgtgagc aagccaatga taagttgcca actggtgcgg
    32341 ttgagttaaa cgtgacagct cttattgtgc ttaacacagc taagacaaca ccatttgaga
    32401 ttaaggatgg cattgaggca aatgacgata cacgtttgcg ttaccgttac cttgaccttc
    32461 gtcgtccaga aatgttggaa aatcttaaac ttcgtgccaa ggtgacccac tctatccgca
    32521 actatttgga tgagttggag tttatcgacg tggagacacc attcctttct aagtcaacgc
    32581 ctgaaggggc gcgtgattat ttagtgccgt ctcgtgttaa taaggggcat ttttacgctc
    32641 ttcctcaaag tccacaaatc acgaaacagc tcttgatgaa tgctggtttt gaccgttact
    32701 accaaatcgt taaatgtttc cgtgacgagg acttgcgtgg agaccgccag cctgaattta
    32761 ctcaggtcga cttggaaacg tccttcctta ctgagaaaga aatccaagat attacagaag
    32821 gcttgatcgc gcgcgtgatg aaggaaacaa aaggcatcga agtaacgcta ccgttccctc
    32881 gtatgaaata cgatgatgct atggctcttt acggttctga caagccagat actcgttttg
    32941 acatgttgct tcaggacttg acagaagtgg tcaaaggtgt agactttaaa gtcttttcag
    33001 aagcacctgc tgtaaaagcg attgtggtca aaggagctac ggacaactat tcacgtaaag
    33061 acatcgacaa gatgacggaa gtagccaaac agtatggtgc caaaggtctt gcttgggtca
    33121 aggtggttga tggagaatta aacggaccag ttgccaagtt cttgactggt atccaagaag
    33181 aattgacaac agcgcttgct cttgaagata aggacttggt tctctttgtg gcggatacgc
    33241 ttgaagtggt taatgcaaca ctgggtgccc ttcgtggacg tattgctaaa gagcttggct
    33301 tgattgataa tgataagttc aacttccttt gggtggttga ctggccgatg tttgaatggt
    33361 ctgaagaaga aggccgctac atgagcgccc accatccatt cacacttcca caggaagaga
    33421 ctgctcatga attagaaggt gatttggcta aggttcgtgc cattgcttac gatatcgtct
    33481 tgaacggtta tgagcttggt ggtggtagcc ttcgtatcaa ccaaaaagac cttcaagaac
    33541 gcatgttcaa ggctcttggt ttctcaactg aagaagccaa tgaccagttt ggtttccttc
    33601 ttgaagccat ggactatggt ttcccaccac acggtggttt ggctatcggg cttgaccgtt
    33661 ttgtcatgtt gcttgctgga gaagaaaata tccgtgaagt cattgccttt cctaagaaca
    33721 acaaggcaac tgacccaatg acacaagctc catcaacagt cgctctcaaa caactagagg
    33781 aactcagctt acaagtagaa gaagatgaaa caaacaaaac gaattaagcg gtggcgctat
    33841 tatctgcgcc gctttgctta tcagataaaa attttacgtg tcttacaaag tatctctcga
    33901 gaaaagtatg atgagaagat ttcggcctct ctggtctatg gttttttatc agcagtagca
    33961 gttaatttct ttttccaacc agggcatgtg tattcgagtg gtgcaacagg tctggcacag
    34021 attatctctg ccttgagtaa tcactggttt ggttttcata ttccgatttc gctaagcttc
    34081 tacgccatta acttcccttt gatggtctta gcttggtatc agattggcca taagttcacc
    34141 gtctttacct ttatcacggt atctatgagt tccttcttta tccagtttgt ccctgtggca
    34201 accttgacgg aggatcccat tatcaattcc ctttttgggg gtgttgttat gggtttgggg
    34261 attggttttg ctcttcgaaa caatatctcc agtggtggga cggatatcgt cagcctgact
    34321 attcgcaaga aaacgggtaa gaatgtcggt agtatttctt tcttggtaaa tggaactatc
    34381 atgctgatag caggtttaac ctttggttgg aaatacgctc tttattctat gattaccatc
    34441 tttgtctcta gccgtgtgac agacgcagtc tttactaagc aaaagcgtat gcaggccatg
    34501 attgtgacaa atcatccaga gaaggtaatt gaaaaaatcc ataaaaaatt gcaccgcgga
    34561 gcaaccatga tccacgatgc agaaggaacc tataatcacg agagaaaggc agttttaatc
    34621 actgtcatta cacgtgcaga gtttaatgaa tttaaacaga ttatgacaca agtggatcca
    34681 agctcctttg tctctgtctc ggaaaatgtt catattctag gaagatttgt tgagatagat
    34741 aattagtgat taatactctt cgaaaatctc ttcaaaccac gtcagcttca tcttgccgta
    34801 tatatgttac tgacttcgtc agttctatct gcaacctcaa aaccatgttt tgagctgact
    34861 tcgtcagttt catctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
    34921 gtttgcactt tgattttcat tgagtataaa aagatcttgg gaaaagactc aatttcagta
    34981 gaaaaggaag taaatcttcc cacaataaaa cgcatagtat caagtttttt caatacctga
    35041 tagtatgcgt ttttgctttt aacgactttt tgcccagtct cttatttttc gataattttg
    35101 tgggcgatca attgacggta acaaatttgt ttattgtttt tattatcatt aataatctgg
    35161 agaatagttt caagggaaac acgaccaagc tctaaactat tgatatcgac ataggctgcc
    35221 aagttgagct tgggattaac cgagtcaaag ctgagaacag ggacatccag ctggtgtttg
    35281 gcaatatagt tacaaacacc ttcagctagg aggctatcgg ttgtgatgat agcatcaatt
    35341 tgtggatcgt gcttgaataa tcgcttgcta aatttatagc ccttttcttc cagaaactcg
    35401 tcggcaaagt agatgcgatt gttgtcagtg gtaagtttgt aatgtttaag cgcctgttca
    35461 tagcctgtta aacggtcttt ggtcacgaag agctttttac ttcctccgat aaaggcaatg
    35521 cgtttgcagc cttttttgat gaaatattca gtcgcatcaa aaccagcttg aacattgtcg
    35581 ttgtcgacaa gtgggatgaa aggagataga gatttaccta agataaggaa ggggaactgt
    35641 tcttctgcga cgagttttac gagagggtct tcttcttggg catagagaaa gattagccca
    35701 tctacacgct tgccgtagac catttgtgaa atagcgttga gacgctcctt ctcatctttc
    35761 cctgttgcta tctgaatggc atagtggttt tcagatgcga cttgagagat gccacgtaga
    35821 accgatggaa agaaaggatt ctggtagaag gcgtctgagt catcaggaag aactaatccg
    35881 ataacctgag tatagctgct taccaagcta cgagcgttga ggtttgggtg gtagttgagt
    35941 tccttcatag ctttgcgaac gcgttttttt gtttcgtcgc taatggttga tttattttga
    36001 ataacacggg ttacggttga aggcgaaaca ccagcagcct tggccacgtc tttaatcgta
    36061 acgggcataa aaatctccta tttatggtaa tttggatcac ggaaatgtgt tttataaaag
    36121 atagtgacca gatacagaac aaaaagaata ttttgtacag taatcagggt tgtcatattt
    36181 tggccaaata atcccaaaat aagtgtaatc agagtcggca atcctaaaca gttcaagata
    36241 aagtgatagc actctttaaa ggttctaaat gaaaagaggc gtgatttctt agtgatataa
    36301 aggagaagac tagctcctag agagacgata aagaaattca aaccaaagag gaagctcgca
    36361 ccgagaacta ggaagaggct gatatagaca cgattttgtt ggtaccagtc tttagaaatt
    36421 gcttgggtca agctgtcttt gcttttgaaa ctctcagtct gaatggctcg gtaagagatg
    36481 cgggtcagct ccttgctttc cttgctgatg actagctcat ttgtatcgaa atgcagttgc
    36541 aagtccttag gtaattcctt gatttgactt ggaccaatca caacagaagg ggtttgacta
    36601 gctgttccag tataagttaa tgtgccatcg acaattgtag catgttcaga gagatcctgg
    36661 acaacctcat ttgtcagagg ttcatagaca ttatcgataa aagtttctag cggataggtc
    36721 tcctgggagc tgttttggat agcaatgggt accatagaca agctgataag gaagatactg
    36781 gtaaagagaa gttgaaacca gttgagctcg aaacgtttgg acaggggctt acgaaacccc
    36841 caaatacttg aaaaatagga aaatggatat ggaagcatag gtatctttct aaaaggtgtt
    36901 ttctatatga gtattattat atagagaaat gcgctttatt tcaagtctag ggggtaaatt
    36961 ccctagtata gcggtatttg tggcttgatt cataaccgat ttataaaatt tgtggacttt
    37021 ttaggaactt tttaattttt caatggcttt ttcataaaat gaagtagcat ttttcttgtt
    37081 gtctttggat aagtggctat aaatatccat ggtcatagaa agttttgcat gaccgagccg
    37141 tgtttgtatc tctttgtaag gtagtccagc attgagcaag atactagcgt gagtatgacg
    37201 gaaagcgtgg aatgttaaac gagggcagtc agaaagtttt aaatgtttct caagtcttga
    37261 cctaagcgcc ctagcatctc tatattcgtc aaaggaatta gaaaatactt tttcataagt
    37321 caagcctatt tctctaccaa cttgcgcttg tctatttttg taaagccgca gcatgagcac
    37381 cgtcttatta tccaagtcaa ttaccctaat acttgattta gtcttagggg tagtgacttc
    37441 tttttcacag tttagagtct tatttacgtc tagtgtaccg ttttgtaagt caatatcaga
    37501 ccatttgagg gctaaacact cacgaatacg caaaccagtc gctaaaagcg tcttatatag
    37561 caccgtatcg tagaaatttt tgtaagtgtt tggcagctgc tccaggtaag tcaagaattt
    37621 ttttaagtta tcatcatcca gatatttgag tttttgccct tcttttgcct tacgccgtgg
    37681 gacgataata tcacgggcag gattaaaagg gataacttgc aaggatacgg catatttaag
    37741 tattcgttta tttagcgagt taagttgcgg atattcttgg taacctttgc ctagttgatt
    37801 gtactctttt gcccactggt ttacctggtg ctgaataatt gggggcgtaa gtttatccag
    37861 cctatagttt ccgaaagctg gaagtagata gttatttagt ctactcttga ttattgtaag
    37921 tgttgccttt ttgactgtat ggcagtacgt ttccagccaa ttctccacca gttcggcgta
    37981 agtctttatc tcaactgctt tatatactgt agaaccgtct ttttcaaagt ctatttgagc
    38041 ctgtagcgct ttggctttga gttctctctt ggttcgccct gatatagtcg tcttgacttt
    38101 cttacctgtt acggtatcga taccaaggta gacgctagag cgatagactg tagaaccgtc
    38161 ttttttggtg tattctgtta ttttcatggt tttactcctt tttccatcag caggcaagca
    38221 attagaaaaa gttttgagtt tataccatgc gaggagctac gagaagcccc ttatttttga
    38281 ttttaattag tcagacggta aataatacca gagtaggaaa caaggcggat atggggctta
    38341 tatgggattt tttaaaagcc catatattgc aagtagttct atcttaatgt gtcagtgata
    38401 ttataccaat tattcggaaa tccaagtgta tccaatacaa tgccggcttc aatggagtgc
    38461 gcttttttat ttagaacttt ggttctttta ataatagtgt tatgtaattg agcgtattgt
    38521 gtggaagcaa gtaggcattg caaggcaaga aaaacataat atacagattg tcttgatgta
    38581 ttggcattgt tattgtaata gtggaaataa gctagttgtg gaagattctc acgacattta
    38641 taattgaaaa gcttattatt atgagcagtg atatttctaa tttctagtat atttttaata
    38701 atactttcta aagcctttcc acttaaaagg tctttactag gtctattttg gatgtattca
    38761 atattttctt gtaagaatgg tgataaatct ctagctattt ggttttttat atcagtgttt
    38821 agatttctat aaaagttaaa tgcttgccct aatgtaagtt cattgatgat aacccagaaa
    38881 ggaacaacgc catgttgttt atagtggtgt tttatagaat tgttttgctt atccctatta
    38941 taatcattga gtattctagc taatatactg attaaattag taatttgtgc tagccttttt
    39001 gtatcttttt gtgtttcaaa attattaata tctagataag cgtatggtgt tggatatgct
    39061 tctgaaaaac aatatgccag tacggattta aagtgttttt ctgcatctat aattgctttt
    39121 aaaaaagctg atttaatttc tttatcgtaa aagtgtgttg cagctatttc tctaaagtct
    39181 gcaccagtta taaatttatc ttttgatgtt tgaaaaaact tactatatcc gttgacgacg
    39241 ttgtaatagt tgttagttag aaggtatctt ttacattctt ctatgttatc tatttctaaa
    39301 cctctagact ggagaagttt tatttgttca tctaatgttt gaaatggttt catatatgtt
    39361 ccttatatac aaaaacacca cctagtagaa ctaggcggtg tttcgcgtac tctgttccct
    39421 tgggaacaga aactcttctc attgcctaat aatactgtaa tttttagttt ttgtcaatct
    39481 ttttggtttg ttttttaaaa atttttgagt atcgaaaaat ctcaacgctg ggaaagttcg
    39541 ctttttttcg tcctaaaact gtcccttccg tgttgtttcg tagtctagtg attctttgaa
    39601 gaggtcagct agtccagtta cgtcttggtt tgcgaggagt tccatataat gcgcccttga
    39661 gtcttttgag acgatgatag gagcgtttag atacttcata gctaggtaca tgagtaaaag
    39721 gcgccctgta cggccgttgc cgtcgctaaa tgggtgaatc ccttcaatct ggatatgggt
    39781 atctgctaaa atctctaaga tttctttttc attctgagca tgatttaagc gataagcggt
    39841 attatcagcc cactgcgtca tcaggtaggg cgtttctgct gggctagccg tttgaaattc
    39901 caccccgata atagcatttt gaacttgctt aaattgtccg cgatcatgtt gcagtctatc
    39961 cactaataag gcgtgaaaat cttgaacgag ccccacagta agggcttgat ggtttgccaa
    40021 tgagttcaag agataagaaa aggcctgttt atggttttca atctcataaa actcacgaat
    40081 acttttgccg ttgcgtggca aggtgctttc taagataatg ctgacggttt cgggtaggga
    40141 aatagtgttc ccttcaatac cgctagaatg gtaggctatg cgtaccagta tgtcgtccag
    40201 gtagtcttga gcgtagttca tgattctcct ttctattctt caatttcctc catttttgca
    40261 caatagacca tgaaacgatg gttgctattg gcgaatatag gggatttttt aatttacgat
    40321 gagttctcca atagggataa tatctttctg ttttgaagat ttagcgatta gatcatattg
    40381 gttagcagat ttttcatacc caagggaaag agtagtattg tcgtctggta attttttagc
    40441 aaactcagat atagacatcc gaaatacggt aattgcattt tgctgattag ttgtagcaga
    40501 atttgaattg attgcgtcca tagtctcttt tgcgctatct ttagccgttc cagttagcaa
    40561 aatcatgatt gtatcatgcg gttcggatga atctgaatcg attacgttat tttgaatttt
    40621 tacgcttatt gcgccagttg attcaggatc taattttaat ttaatttcag agattaaatc
    40681 atcatattta ctgttgtcta ctttggcttt tgtatttgtt gaagtagtgt ttttttgctc
    40741 agtttttggt tgctccgagc tatctttggt agctgattga ttgttagagc aagctactag
    40801 aacggtagca gaaagtaaaa tagctgatgt gcttagtaat tttttcataa gtagcctcct
    40861 atatgctgat gttttaaaag atggggaatt tttatagaat tttctcaaaa accattgtgg
    40921 cttggatacg gtcaccaccg cctagtcctt tgcttccacc attggcggtt gtgattgtat
    40981 gcaggcgata accttttgaa gcttgtttat tgataacatc ttctaattct gtaaggtttc
    41041 ctgatccagt accgaaaaat ttttctttca aagttacctg aaggacaacg tagtgtagtc
    41101 catttactcc agatgcagta gaaaagcttc cttcttgttt tacagtgtca aaaaatccca
    41161 tgagagttac tccttttatt atgttagtaa ttgataaaaa ttagtcaatg atttttttgt
    41221 attgttcgaa ttgttcttct attg
//
LOCUS       DAVGJJ010000008        39280 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000008 DAVGJJ010000000
VERSION     DAVGJJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.008"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            2..1279
                     /locus_tag="VVE35_000396"
     CDS             2..1279
                     /locus_tag="VVE35_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU3060648.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1355..2839
                     /locus_tag="VVE35_000397"
     CDS             1355..2839
                     /locus_tag="VVE35_000397"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU3060649.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQATTGDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            2900..4180
                     /locus_tag="VVE35_000398"
     CDS             2900..4180
                     /locus_tag="VVE35_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU3060650.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIVLVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            4184..4804
                     /locus_tag="VVE35_000399"
     CDS             4184..4804
                     /locus_tag="VVE35_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3060651.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            4970..6112
                     /locus_tag="VVE35_000400"
     CDS             4970..6112
                     /locus_tag="VVE35_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU3060652.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAVVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6540..6965
                     /locus_tag="VVE35_000401"
     CDS             6540..6965
                     /locus_tag="VVE35_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU3060653.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            6976..7335
                     /locus_tag="VVE35_000402"
     CDS             6976..7335
                     /locus_tag="VVE35_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU3060654.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7464..8060
                     /locus_tag="VVE35_000403"
     CDS             7464..8060
                     /locus_tag="VVE35_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU3060655.1"
                     /translation="MSPFEAIKGLPAEIHGAEVRWLEVPTVFHKSAQVLEEEMNRYQP
                     DFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPIRPDGASAYFSSLPIKA
                     MVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFSYVKAGFMHIPYMMEQVVNR
                     PTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(8088..8945)
                     /locus_tag="VVE35_000404"
     CDS             complement(8088..8945)
                     /locus_tag="VVE35_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU3060656.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9075..10217
                     /gene="tgt"
                     /locus_tag="VVE35_000405"
     CDS             9075..10217
                     /gene="tgt"
                     /locus_tag="VVE35_000405"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU3060657.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            10318..12135
                     /locus_tag="VVE35_000406"
     CDS             10318..12135
                     /locus_tag="VVE35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU3060658.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12288..13439
                     /gene="nagA"
                     /locus_tag="VVE35_000407"
     CDS             12288..13439
                     /gene="nagA"
                     /locus_tag="VVE35_000407"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU3060659.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            13603..14661
                     /locus_tag="VVE35_000408"
     CDS             13603..14661
                     /locus_tag="VVE35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU3060660.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            14812..15177
                     /locus_tag="VVE35_000409"
     CDS             14812..15177
                     /locus_tag="VVE35_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU3060661.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15253..16194
                     /locus_tag="VVE35_000410"
     CDS             15253..16194
                     /locus_tag="VVE35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU3060662.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16142..17158
                     /locus_tag="VVE35_000411"
     CDS             16142..17158
                     /locus_tag="VVE35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU3060663.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTIITQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17160..17486
                     /gene="comGC"
                     /locus_tag="VVE35_000412"
     CDS             17160..17486
                     /gene="comGC"
                     /locus_tag="VVE35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU3060664.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGA
                     NRKVND"
     gene            17455..17883
                     /locus_tag="VVE35_000413"
     CDS             17455..17883
                     /locus_tag="VVE35_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU3060665.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQVPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            17888..18148
                     /locus_tag="VVE35_000414"
     CDS             17888..18148
                     /locus_tag="VVE35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060666.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18111..18572
                     /locus_tag="VVE35_000415"
     CDS             18111..18572
                     /locus_tag="VVE35_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU3060667.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18550..18963
                     /locus_tag="VVE35_000416"
     CDS             18550..18963
                     /locus_tag="VVE35_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU3060668.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19100..19694
                     /locus_tag="VVE35_000417"
                     /pseudo
     CDS             19100..19694
                     /locus_tag="VVE35_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            19755..20708
                     /locus_tag="VVE35_000418"
     CDS             19755..20708
                     /locus_tag="VVE35_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3060669.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            20759..21949
                     /locus_tag="VVE35_000419"
     CDS             20759..21949
                     /locus_tag="VVE35_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU3060670.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22098..22469
                     /gene="rnpA"
                     /locus_tag="VVE35_000420"
     CDS             22098..22469
                     /gene="rnpA"
                     /locus_tag="VVE35_000420"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU3060671.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22444..23268
                     /locus_tag="VVE35_000421"
     CDS             22444..23268
                     /locus_tag="VVE35_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU3060672.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23287..24273
                     /locus_tag="VVE35_000422"
     CDS             23287..24273
                     /locus_tag="VVE35_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU3060673.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24324..24947
                     /locus_tag="VVE35_000423"
     CDS             24324..24947
                     /locus_tag="VVE35_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU3060674.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKVFQANHPEWQLQQTSQWMNQAQRLRPHFWVYLQREGKVTEPMIALRLYGNPSDF
                     GISLEVSFIEHKKDEQTLEKQAKILDIPTVKGIYYLAYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25078..26535
                     /locus_tag="VVE35_000424"
     CDS             25078..26535
                     /locus_tag="VVE35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU3060675.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26558..26839
                     /locus_tag="VVE35_000425"
     CDS             26558..26839
                     /locus_tag="VVE35_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3060676.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            26916..27401
                     /locus_tag="VVE35_000426"
     CDS             26916..27401
                     /locus_tag="VVE35_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3060677.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27418..28083
                     /locus_tag="VVE35_000427"
     CDS             27418..28083
                     /locus_tag="VVE35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU3060678.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            28087..28950
                     /locus_tag="VVE35_000428"
     CDS             28087..28950
                     /locus_tag="VVE35_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU3060679.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            28952..29656
                     /locus_tag="VVE35_000429"
     CDS             28952..29656
                     /locus_tag="VVE35_000429"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU3060680.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            29835..31508
                     /locus_tag="VVE35_000430"
     CDS             29835..31508
                     /locus_tag="VVE35_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU3060681.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            31620..32711
                     /gene="ulaG"
                     /locus_tag="VVE35_000431"
     CDS             31620..32711
                     /gene="ulaG"
                     /locus_tag="VVE35_000431"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU3060682.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDIIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            32825..34801
                     /gene="tkt"
                     /locus_tag="VVE35_000432"
     CDS             32825..34801
                     /gene="tkt"
                     /locus_tag="VVE35_000432"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU3060683.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            34918..35217
                     /gene="yajC"
                     /locus_tag="VVE35_000433"
     CDS             34918..35217
                     /gene="yajC"
                     /locus_tag="VVE35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU3060684.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35264..35692
                     /locus_tag="VVE35_000434"
     CDS             35264..35692
                     /locus_tag="VVE35_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU3060685.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            35694..36104
                     /locus_tag="VVE35_000435"
     CDS             35694..36104
                     /locus_tag="VVE35_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060686.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILTVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36404..39055
                     /gene="adhE"
                     /locus_tag="VVE35_000436"
                     /gene_synonym="adhC"
     CDS             36404..39055
                     /gene="adhE"
                     /locus_tag="VVE35_000436"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU3060687.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGSGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMGVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        11977 a         6989 c         8812 g        11502 t
ORIGIN      
        1 tatgtcttgt tctatcgatt tattaaaaca tcggtatttg aaaaatatta aagaaaatcc
       61 tgaattgttt gtcggaattg agttggagta tcctgttgca agtttagaag gggatgctac
      121 agatgttgaa gttataaagg atctatttca ttatttagtt tctactttgg atctcaccgt
      181 agcaaaggta gatgattttg gcaatctgat ccagttagta gatccgataa gtcaggatgc
      241 tattttattt gaagtttcct atacaacgat tgagtttgca tttggtaagg ctgaaacgat
      301 tcaagaggtc gaaaatcgtt tcaataatta tatgaatgta attcagagaa agttagctga
      361 atcaaatcat gctattgttg gctgtggtat ccatcccaac tgggataaaa atgagaattg
      421 tccagtggct tatccacgct atcagatgtt gatggattat ttgaatttga gtagaaatat
      481 tattaaatca gatttacatc atttccctga atatggtact tttatctgtg ggagccaggt
      541 tcagctggat atttcaaaaa ccaactactt gcgggtgatt aatgctttta ctcaaattga
      601 agcggctaag gcttatttat ttgcaaactc tgagttttcg ggtgcggatt gggatacgaa
      661 aatttcaagg gatattttct gggaagaatc tatgcatggt atctatccag agaatgttgg
      721 ggtcaatgct agactcctta atgatgaagc tgattttttt gactatctaa atcattctgc
      781 gatttttact gcggaacgtg atgggcagac ctattatttt tatcctattc aggctgggga
      841 ctatttggct acgcctgaaa tccaagcatt tgctctgaat ggggatgagg ttattattta
      901 cccccaagag aaggattttg aaactcatcg tagttaccag taccaagact taacgactcg
      961 aggaacagtt gagtttcgta gtgtgtgtac acagccactt gataggactt ttgcttctgc
     1021 agcttttcac ttgggattat tggttaattt agacaagtta gaagcttact tagaaacagc
     1081 acctttcttt aaagtatttg gttatgatta caagttttta aggagacaat tttctaagaa
     1141 aaatcttaca gatgaggaag aaactatgat tattgaattt tccaaagact tactcctact
     1201 agctgaggag ggactagtgg tgagaaataa ggaagaaatg acctatttac agcctttgag
     1261 agaagaattg agcctataat ttctcttata aagggagaat tttctgaaaa atcatgatat
     1321 aatggacgag actatagata aaggatagag agtaatgaca ttagtttatc aatcaacgcg
     1381 tgatgccaac aatacagtaa ctgccagcca agcaattttg caaggtttgg cgacggacgg
     1441 cggtttgttt acaccggtta cttatccaaa ggtagatttg aactttaaca aattgaaaga
     1501 tgcttcttac caggaagttg ctaagctagt tttgtcagca tttttagatg actttacagc
     1561 tgaggagttg gactactgta tcaacaatgc ctacgatagc aaatttgata ctccagctat
     1621 tgcaccatta gtgaaattag atgggcaata caatttggaa cttttccatg gttcaacgat
     1681 tgcctttaag gatatggcct tgtctatttt gccatacttt atgacgactg ctgctaagaa
     1741 acatggtttg gagaacaaga ttgttatctt gacagcgaca tctggtgaca cggggaaagc
     1801 tgctatggcg gggtttgcgg atgtgcctgg tactgagatt atcgtctttt atccaaagga
     1861 tggtgtcagc aagattcaag agttacagat gaccactcaa actggcgaca atactcatgt
     1921 tattgctatt gatggtaact ttgacgatgc gcaaacaaat gtgaagcaca tgtttaacga
     1981 cgtggctctt cgtgaaaaat tgactaccaa caagttgcaa ttttcatcag ctaactctat
     2041 gaacattggt cgtctggtgc cacaaattgt ttattatgtt tatgcttacg ctcaattggt
     2101 caagactggt gagatcgtgg ctggtgataa gattaacttc acagtaccaa caggaaactt
     2161 tggaaatatc ttggctgcct tttatgccaa acaaatcggt ctcccagttg gtaaattgat
     2221 ctgtgcttca aatgacaaca atgttttgac agacttcttc aagacacgtg tctatgacaa
     2281 gaaacgtgag tttaaggtaa caaccagccc atctatggat atcttggtat cttcaaactt
     2341 ggagcgtttg attttccatc ttttggggaa tgatgcggtt aagacggctg aactcatgaa
     2401 tgctttgaat agccaaggtc aatatgagtt gactaatttt gatgcagaga ttttggacct
     2461 ctttgcagct gaatatgcga ctgaggaaga aactgcggca gaaatcaagc gtgtttatga
     2521 gtcagattct tatatcgagg atcctcatac agcggttgcc tcagcagtgt atagaaaata
     2581 ccaggcgact actggagatg cgactaagac agtgattgct tcaacagcta gtccctacaa
     2641 gttcccagta gttgcagtag aagcgctaac agggaaagtt ggcttgactg attttgaagc
     2701 tttggctcaa ttacatgaaa tctcaggcgt tgcagtgcca ccagcagttg atgggcttga
     2761 gacagctcct gttcgtcaca agacaatagt ggcagctgct gacatgcaag tagcggttga
     2821 ggcttattta ggactttaag atagagggag taaactcggt tgggaaacca actgagtttc
     2881 ttttcatcag gaggagagat tgtttaagaa aaataaagac attcttaata ttgcattgcc
     2941 agctatgggt gaaaactttt tgcagatgct aatgggaatg gtggacagtt atttggttgc
     3001 tcatttagga ttgatagcta tttcaggggt ttcagtagct ggtaatatta tcaccattta
     3061 tcaggcgatt ttcatcgctc tgggagctgc tatttccagt gttatttcaa aaagcatagg
     3121 gcagaaagac cagtcgaagt tggcctatca tgtgactgag gcgttgaaga ttaccttact
     3181 attaagtttc cttttaggat ttttgtccat cttcgctggg aaagagatga taggactttt
     3241 ggggacggag agggatgtag ctgagagtgg tggactgtat ctatctttgg taggcggatc
     3301 gattgttctc ttaggtttaa tgactagtct gggagccttg attcgtgcaa cgcataatcc
     3361 acgtctgcct ctctatgtta gttttttatc caatgccttg aatattcttt tttcaagtct
     3421 agctattttt gttctggata tggggatagc tggtgttgct tgggggacaa ttctgtctcg
     3481 tttggttggt cttgtgattt tgtggtcaca attaaaactg ccttatggga agccaacttt
     3541 tggtttagat aaggaactgt tgaccttggc tttaccagca gctggagagc gacttatgat
     3601 gagggctgga gatgtagtga tcattgtctt ggtcgtttct tttgggacgg aggcagttgc
     3661 tgggaatgca atcggagaag tcttgaccca gtttaactat atgcctgcct ttggcgtcgc
     3721 tacggcaacg gtcatgctgt tggcccgagc agttggagag gatgattgga aaagagttgc
     3781 tagtttgagt aaacaaacct tttggctttc tctgttcctc atgttgcccc tgtcctttag
     3841 tatatatgtc ttgggtgtac cattaactca tctctatacg actgattctc tagcggtgga
     3901 ggctagtgtt ctagtgacac tgttttcact acttgggacc cctatgacga caggaacagt
     3961 catctatacg gcagtctggc agggattagg aaatgcacgc ctcccttttt atgcgacaag
     4021 tataggcatg tggtgtatcc gcattgggac aggatatctg atggggattg tgcttggttg
     4081 gggcttgcct ggtatttggg cagggtctct cttggataat ggttttcgct ggttatttct
     4141 acgctatcgt taccagcgct atatgaactt gaaaggatag gaaatgcaaa aaacagcttt
     4201 tatttgggat ttagacggga ctttattgga ctcttacgaa gcgattttat cagggattga
     4261 ggagactttt gctcagtttt ctattcctta tgataaggag aaggtgagag agtttatctt
     4321 caagtattcg gtgcaagatt tgcttgtgcg ggtggcagaa gatagaaatc tggatgttga
     4381 ggtgctaaat caggtgcgtg cccagagtct ggctgagaag aatgctcagg tagttttgat
     4441 gccaggtgcg cgtgaggtgc tagcttgggc agacgaatca ggaattcagc agtttatata
     4501 tactcataag gggaacaacg cttttaccat tctcaaggac ttgggggtgg aatcctattt
     4561 tacagagatt ttaaccagtc agagtggctt tgtgcggaag ccaagtccag aagcggctac
     4621 ctatctgcta gataagtatc agttgaattc tgataatact tattatatag gggatcggac
     4681 tctggatgtg gaatttgccc agaatagtgg gattcaaagt atcaactttt tagagtctac
     4741 ttatgaaggg aatcacagga ttcaagcgtt agcagatatt tcccgtattt ttgagactaa
     4801 gtgataaaaa gattgtgtca gttttgtgac agagacctaa caaactattt caagtaacct
     4861 agtttgttac aaggaataga cagttctgtt aaataggccc gagagggctt tttttctaca
     4921 ttttttgtgt tatcatagac aggtactcat ttgaaaggaa tttgaaagaa tgaagaaaag
     4981 aatgttatta gcgtcaacag tagccttgtc atttgcccca gtattggcaa ctcaagcaga
     5041 agaagttctt tggactgcac gtagtgttga gcaaatccaa aacgatttga ctaaaacgga
     5101 caacaaaaca agttataccg tacagtatgg tgatactttg agcaccattg cagaagcctt
     5161 gggtgtagat gtcacagtgc ttgcgaatct gaacaaaatc actaatatgg acttgatttt
     5221 cccagaaact gttttgacaa cgactgtcaa tgaagcagaa gaagtaacag aagttgaaat
     5281 ccaaacacct caagcagact ctagtgaaga agtgacaact gcgacagcag atttgaccac
     5341 taatcaagtg accgttgatg atcaaactgt tcaggttgca gacctttctc aaccaattgc
     5401 agaagctcca aaagaagtag catcaagttc agaagttaca aagacagtga ttgcttctga
     5461 agaagtggca ccatctacgg gcacttctgt cccagaggag caaacggccg aaacaactcg
     5521 cccagttgaa gaagcaactc ctcaggaaac gactccagct gagaagcagg aaacacaagc
     5581 aagccctcaa gctgcatcag cagtggaagt aactacaaca agttcagaag caaaagaagt
     5641 agcatcatca aatggagcta cagcagtagt ttctacttat caaccagaag agacgaaaat
     5701 aatttcaaca acttacgagg ctccagctgc gcccgattat gctggacttg cagtagcaaa
     5761 atctgaaaat gcaggtcttc aaccacaaac agctgccttt aaagaagaaa ttgctaactt
     5821 gtttggcatt acatccttta gtggttatcg tccaggagac agtggagatc acggaaaagg
     5881 tttggctatc gattttatgg taccagaacg ttcagaatta ggggataaga ttgcggaata
     5941 tgctattcaa aatatggcca gccgtggcat tagttacatc atctggaaac aacgtttcta
     6001 tgctccattc gatagcaaat atgggccagc taacacttgg aacccaatgc cagaccgtgg
     6061 tagtgtgaca gaaaatcact atgatcacgt tcacgtttca atgaatggat aaacccgact
     6121 tgataacatc attttgacga atgagatcta gctttcgtga tggaaagcga ttctcgttcg
     6181 ttttttcttt gtcatactct tcgaaaatct cttcaaacca cgtcagtttt atctgaaacc
     6241 tcaaagctgt gctttgagca acctgcgact agcttcctag tttgcttttt gattttcatt
     6301 gagtatcaat ttgaatggaa aatggaaagt tattatcttg taatgagtta agcaacattc
     6361 ttgcaatcta ttttatagta gatggaaact agaatagtac acaactgctt ctaaaacatt
     6421 gttagaaatc gatttgactg tcctgatcga tttgtcctgc tcttatttca tttcactata
     6481 ctttatatta taattgactg gtcaactatt gataaatcaa taaaaagaga ggggaagaaa
     6541 tgatagagat tcaagattta ctgtatcaac tccgcttgtc tgagcaagcg agtacgcaat
     6601 tgtttgaaaa aaggcttggg attagtttga cacggtatca gattttactg tttttgctgg
     6661 agcattctcc ttgtaaccaa atggcggttc aggagcgttt gaaaattgat caggctgctt
     6721 tgacacggca tttcaaaatt ttggaaacgg aaggtttggt ggagcgtcat cgtaatcctg
     6781 aaaatcagcg ggaagtgttg gtagaggctg cgaagtatgc caaggagcag ttagtggtga
     6841 atccccctct gcaacatatc agggttaagg aagagataga aagtatctta acagagtttg
     6901 agagaacaga actcagccgt ttattaaata aattggtttt gggtattgaa aatatagaaa
     6961 tttaaggaga aatagatgtc aattatttta acaacgatcg ttgctttgga gcatttttac
     7021 attttttatt tggaaagtat tgccacgcaa tcagatgcga ctagtcgtgt atttaatatg
     7081 gaaaaggaag aactggctca tccgtcagta agttcattgt tcaaaaatca aggaatttat
     7141 aaggctctgc taggagtctt tctcttgtat gtcatttatt tctcacagaa tttagaaatt
     7201 gtgactattt ttgtcttatt tgtgattggt gctgcgactt acggctcttt aacagcggat
     7261 aaaaaaatta ttttgaaaca aggtggatca gctattttgg ccttgattag tattttactc
     7321 tttaaataca cttgaaggtc gattctaatc tcgctaatcc ttttcaatcc agaataaggg
     7381 aaatatgtta tacttgtttt taagaaaaaa gtttcattga attggttttg aggagttaga
     7441 aatgaaagta ttagtgacag gttttgagcc cttttgaggc cattaaaggt ttaccagctg
     7501 aaatccatgg tgctgaggtc cgttggctag aggtgccgac agtttttcac aaatctgctc
     7561 aagtattgga agaagagatg aatcgttatc aacctgactt tgtcctttgt attgggcaag
     7621 ctggtggaag aactagtttg acacctgaac gagtggccat taatcaagac gatgcacgta
     7681 cttctgataa cgaagataat caaccgattg accgtcccat tcgcccagat ggtgcttcgg
     7741 cctactttag tagtttgccg attaaagcga tggttcaagc tataaaaaaa gagggcttac
     7801 cggcctctgt ttccaatacg gcagggactt ttgtctgcag ccatttgatg tatcaggctc
     7861 tctatttggt agaaaagaaa ttttcatatg ttaaggcagg ttttatgcat attccttata
     7921 tgatggaaca ggtggtgaac agaccgacta ctccagctat gagtttagtg gatattcggc
     7981 gagggataga agcagcaatc ggcgctatga tagaacatgg agatcaggaa ctcaagttgg
     8041 taggcggaga aactcattga tagaaaaaag cttgagggga aaaaccttca agcttttgga
     8101 cgttttcgag ccaatactgc tcggtaaaac ataattttag tgcattggat ataaggtagg
     8161 agtgaaaaac tagcaatgcc aaaggtaatc caattgagga agtaccaagg aagaagctgt
     8221 aaatctagga caaagcgctg gaacttgtag cccttcataa aggaacggct agtttttagg
     8281 attcgtcttg gtgggacctg tcctaggtct agactataac agagaagaaa ttccacctgt
     8341 gaataggcat aatactgtgg aatatagagg atatttccta caatgatcaa gatgagactt
     8401 gcaagaaagt agagtccaaa gaccatgagg aaacgctcgg tttcaactga tgagagatct
     8461 agatttggaa actcaggatg tagggtgacg aattttttgg ctaaaaagct actataaaag
     8521 aggaggtaaa tcccaagtaa attagggata ctccataaaa agagatagaa acgtttgaga
     8581 agtagggtca aaaaggtttg agaaaagcgc tcctcatcaa agagagctag gctgtttttt
     8641 acagatggct ccgttttaga atctttcatg agtgtcagtg ttgcatagac ggaactggtc
     8701 aaaagaatag tcccgataaa ggagactagt agaggaaaga ggtaggcttg aagtatttgg
     8761 ccaagtatgc tgaaaaatgg ctgttctaaa acagtcccgt ggatccgaga taagggatta
     8821 agaaaactag ataagatgac cagcatactg ggaaggatat agaggagaaa gagacggggg
     8881 gtgtcagcct gaaaatgttt tgactcctga cgaattgttt ttaaatcaat ttttggatag
     8941 ttcattctct tattatacca tagttcttat acatagttcg tgacagttcc tacttttttt
     9001 gataaaatca tacagtgtgt ccttgggcac actgtatgaa ctgggactgt ctttcccagc
     9061 ttcggaggta aaaaatgtca gattcaccaa tcaaatatcg tttgattaag aaagaaaaac
     9121 acacaggagc tcgtctagga gaaatcatca ctccccacgg tacctttccg acacctatgt
     9181 ttatgccagt tgggacacaa gccactgtca aaactcagtc acctgaagaa ttgaaggaga
     9241 tgggttcggg aattatccta tcaaacacct atcatctctg gcttcgccct ggagatgaac
     9301 tcattgcacg cgctggtggt ctccacaagt tcatgaattg ggaccagcct atcttgacag
     9361 atagtggtgg ttttcaggtt tattctttag cagatagccg taatatcaca gaagaaggag
     9421 taacctttaa aaatcatcta aatggttcta agatgttcct atccccagaa aaagccatct
     9481 ctattcagaa taatctgggt tcagacatca tgatgtcctt tgatgaatgt cctcagtttt
     9541 atcaacctta tgactacgtt aagaaatcga tcgagcgtac cagccgttgg gctgagcgtg
     9601 gtttgaaggc tcaccgtcgt ccacatgacc aaggtttgtt tggaattgtg caaggtgcag
     9661 gatttgaaga ccttcgccgc caatcagctc atgatcttgt cagcatggat ttctcaggct
     9721 actctatcgg tggtttggca gtgggagaaa cccatgaaga gatgaatgcg gtcttggact
     9781 ttacaactca actgctgcct gaaaataaac ctcgttatct gatgggtgtg ggagcgccag
     9841 atagcttgat cgatggggtc attcgtgggg tggatatgtt tgactgtgtc ttaccgactc
     9901 gaattgctcg taacgggact tgtatgacca gtcaaggacg tttggttgtg aaaaatgccc
     9961 agtttgctga ggactttacg ccactggatc ctgagtgtga ttgctacaca tgtaataact
    10021 atacacgcgc ttaccttcgt cacctgctca aggctgatga aacctttggt atccgcttga
    10081 ctagctacca caatctttac ttcttgctta acctgatgaa gcaagtgcga caagccatca
    10141 tggatgacaa tctcttggaa ttccgtaagt attttgtgga aaaatatggc tataataagt
    10201 caggacgtaa tttctaaaat ggaattgata taagctaaaa atcctaagtt ttctcttagg
    10261 atttttcttc tttttttgat agaataaagt gtacaatgaa aggaagaata aactcgtatg
    10321 cgcattaaat ggttttcctt gattaggatt ataggtttac ttttggtact cttgtatcac
    10381 ttctttcaga cgatctttcc tggaggattt ttcggggtag atgtcttttt cacattttca
    10441 ggtttcctga ttacagctct actcattgaa gaattttcta agaacaatga gattgatttg
    10501 ataggatttt ttagaagacg cttttatcgg attgtgccac ctgtggtttt gatggtcttg
    10561 gtgaccatgc cttttacttt cttggttcgc caagactatg ttgctggaat tggtggccag
    10621 attgcgggcg tcttaggctt tatgaccaac ttctatgaac tcttaacagg tgggagttat
    10681 gaatctcagt tcattcctca tttgtttgtt cataattgga gtttggcagt tgaggttcac
    10741 tactatattc tttggggatt ggcagtttgg ttcttatcca aacaagctaa atcaaatggt
    10801 cagttgaagg ggatggtctt tctcttatct gctgctgcct tcttgatcag tttcttctcc
    10861 atgtttattg gtagttttct agtgacctct tattcctctg tttatttctc cagtttaact
    10921 catgtctatc cattcttttt gggaagtatg ttagcaacta ttgtaggcgt tcgtcagacg
    10981 acttccctcg tcaagcagtt ggataaaatc tgggatttac gaaagacttt ggtagttttt
    11041 ggaggaggct ttggtttcct agttcttttg actttctttg tcaaattcac ttatcttttt
    11101 gcctatctta tcggcttctt gcttgccagt cttgcagctc ttgccatgat tctggcggcg
    11161 cgtgtcttac atgaaaagac acatcatata caggagccga agattatcag ctttttagcg
    11221 gatactagct atgcggttta tcttttccat tggcctttct atatcatttt ctcacagttg
    11281 acatcaaatc ttcttgctgt attactgact ctgatttgtt cttatggctt tgccagtctg
    11341 tcattttatg tattggaacc ttggattgca ggcaagaaca cacctatttt acaaactctt
    11401 cgtcccctgc cttatattca cgcaattctt gcagcaggta caggaatctt gaccttcatt
    11461 gtctgcatgg tgaccttgtt ggcaccacaa gtgggagcgt ttgagacaga cttgactgtc
    11521 aatggcttga agcaagctgc aacaaatatt ggccagacca aggtgatggc agaacgggca
    11581 gatgcaaaca gtttgggaat tgctgatggc actatgttaa ttggtgactc agtggcttta
    11641 agggcaaata cagcactaca gacagctctt cctggagcac agattaacgc gcaggtcagc
    11701 gtaacaacca agaccgcaaa tgaaatcatg ctaaataata gccagaataa atttttacct
    11761 aagacggtgg tcattgcgac tggggtaaat aatcctgaga attacaagga tgactgggac
    11821 agtatcgtga aaaatcttcc taagggacac cgtatgattt tggtgactcc ttatgaggga
    11881 gataagacaa aagagaccta tgccatcgtt gagaaggctg ctgcctatat gagagaattg
    11941 gcagagaaga caccttacat tacgatagca gattggaatc aagttgcgaa agagcatcca
    12001 gaaatttggg caggaacaga ccaggttcat ttcgggagtg agagtagcac tatcgaagca
    12061 ggagcaaaat tgtatgcaga tacgattgcc acagctttgc agacagctca agacaagccg
    12121 gttaaatcaa aataacttgt attaaaaaag agaagagttg gagaaatcca gctcttttaa
    12181 aatatctcca aaaaataggt ctataccatt tacaaataaa aaagaaaggt ttataatgta
    12241 attgacataa taaattgtag aatcaatctt ttaaggaggt taacattatg cctaactata
    12301 ttaaagcgga tcagtttttc tacccacacg gagttcgtcg aggtggttac ttggaacttg
    12361 tggacggcaa gtttgggaaa catgtagagc agattcctga aggggctgag gtgattgact
    12421 atacaggtta tagcattgcc ccaggtcttg tggatactca tattcatgga tatgcaggtg
    12481 tagatgtgat ggacaacaac attgaaggta cattgcatac tatgagtgaa ggacttctta
    12541 gtaccggtgt taccagtttc ttacccacaa ctttaacagc cacttatgag caattgcttg
    12601 cagtcactga aaatcttgga aaccattata aagaagcaac aggtgctaag attcgtggga
    12661 tttattatga aggtccatat ttcacagaaa cttttaaggg ggcacaaaat ccaacttata
    12721 tgagagaccc gggtgttgag gagtttcatt cttggcaaaa agcggcaaat ggcttgctta
    12781 ataaaattgc ccttgcacca gaacgtgatg gggtggaaga ctttgttcgt acagttacgg
    12841 gcgaaggtgt gacggttgct cttggacatt caaacgcgac ttttgatgaa gccaaaaaag
    12901 cagtcgatgc tggagcgagt gtttgggtgc atgcctacaa tggaatgcgt gggttgactc
    12961 accgtgaatt gggtatggtt ggagccatgt accaattgcc acatacctat gcagagttga
    13021 tctgtgatgg tcaccacgta gatccaaagg cttgcgaaat tcttatcaaa caaaaaggaa
    13081 cagaaaacat cgctcttatc acagactgta tgacagctgg gggattggaa gacggagatt
    13141 atatgttggg agaattccca gttgtcgttg caaatggaac tgcacgcctc aaatcgacag
    13201 gtaacttggc aggttctatc ctcaaactca aagatggttt gaagaatgtg gtcgaatggg
    13261 gaattgcgaa tccgcatgaa gcagtcatga tggccagctt caacccagct aaatccgttc
    13321 acatcgatga cgtctgtggc caaatccgtg aaggctacga cgctgacttc atcgtattag
    13381 ataaagattt ggaattggta gcaacctacc tagatggcgt gaaacgttat caagcataag
    13441 agagaaagca gaagttagag actaacttct gcttttttta tacagggtac tttactggta
    13501 aataaaaagt taaaaaaatc acaaaaaaag cttgaaaaaa caaagaaaaa tgcttacaat
    13561 tgaactataa atattacaaa taaaaaaacg gaggagtgct ttatgaaagc ctatacttat
    13621 gttaaaccag gacttgcttc ttttgttgat gtagacaaac cagttattcg caagccaaca
    13681 gatgctattg tgcgtattgt aaaaaccact atttgtggaa cagacctcca tattatcaaa
    13741 ggggatgttc ctacttgcca aagtggtacc attcttggcc acgaagggat tgggattgtt
    13801 gaagaagttg gggaaggagt ttccaacttc aaaaaaggtg acaaggtctt gatttcttgc
    13861 gtctgtgcct gtggtaaatg ctactactgt aaaaaaggaa tttatgccca ctgtgaagac
    13921 gaagggggct ggattttcgg tcacttgatt gatggtatgc aggctgaata tctacgtgtc
    13981 cctcatgcag ataatactct ttaccatact ccagaagact tgtcagatga agctttggtt
    14041 atgctgtcag acattctgcc tactggatat gaaattggtg tcttaaaagg gaaagtagaa
    14101 cctggttgca gcgtagccat tattggttca ggtccagttg gattggctgc tcttttaaca
    14161 gcccaattct attcaccagc taaattgatt atggtagacc tagacgataa ccgcttggaa
    14221 actgccctat cattcggtgc gactcataag gttaattctt cagaccctga aaaagccatt
    14281 aaagaaattt atgatttgac agatggtcgt ggtgtggatg tcgctatcga agctgttggt
    14341 attcctgcaa catttgattt ctgtcaaaag attatcggtg tagacggaac ggttgccaac
    14401 tgtggtgtgc atggtaaacc agttgaattc gatttagata aactttggat tcgcaacatc
    14461 aatgtaacaa ctggtttggt atctacaaat acgactccac aattgttgaa agcacttgaa
    14521 agtcataaga ttgaaccaga aaaattggta actcactatt tcaaactcag tgaaattgaa
    14581 aaagcctacg aagtcttcag taaggcagca gaccaccatg ccattaaggt cattatcgaa
    14641 aacgatatct cagaagccta agtagtaaaa atatttttgt acataagtaa atagaaattc
    14701 agtcatccat cagatggctg gattttttat caaaaaatta agaaatgagc atatttcttt
    14761 ccttgtctgg cggaattggt tataatatac ggtacaaagg aatgaatgaa tatgtatcgt
    14821 gttatagaaa tgtacggaga ttttgaaccg tggtggttct tagaaggttg ggaagaagat
    14881 attgtagcaa gtagaaaatt tgaccagtat tatgatgctc tcaaatacta caaaacttgc
    14941 tggtttagat tggaacaaga atcccctctt tataaaagta gaagcgactt gatgaccatt
    15001 ttttgggacc cggaagacca acgctggtgt gatgaatgtg atgagtattt acaacaatac
    15061 cattctttgg ctcttttgca ggatgagcag gttatcccag acgaaaaact acgctcaggc
    15121 tatgaaaaac aaaccagtca ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga
    15181 aaagtaactt ttttggagtt gcttttttta tttttctaac gctttgcgaa tagtataggt
    15241 gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcagc tcggaaaaaa
    15301 gggacgcagg atatttattt tgtccctaag ttagacgcct atgagcttca tatgagggta
    15361 ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc cgttatcagt
    15421 cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc
    15481 tgtgattatg cctatgacca gaagatggcg tctctacgtt tatctactgt aggcgattat
    15541 cggggacatg agagtttggt tatccgtttg ttgcacgatg aggagcagga cttgcatttt
    15601 tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact ctatcttttt
    15661 gctggcccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa gtcactcttt
    15721 aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga cgacatgctt
    15781 cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa actttccttg
    15841 cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac ggcgcgtgca
    15901 gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc caagagtatc
    15961 cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt
    16021 ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga ctttgcaaac
    16081 agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca gcttcttaaa
    16141 gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa
    16201 aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg tggagactat
    16261 ctccttttta gataggagtg ctttgttgga caagcagtgt gtgatccaga tgcgtgcggg
    16321 cttgtctcaa gggaaatcat tctcagaaat gatggaaagt ttgggatttt caagcaccat
    16381 tatcactcag ttatccctag ctgaagttca tggaaatctc cacctgagtt tgggaaagat
    16441 agaagaatat ctggacaatc tggctaaggt caagaaaaaa ttgattgaag tagcgaccta
    16501 tcctttgatt ttgctgggtt ttcttctctt aattatgctg gggctacgga attacctgct
    16561 cccacaactg gatagtagca atattgccac ccaaattatc ggtaatctgc cccaaatttt
    16621 tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt tttataaaag
    16681 aagttctaag atgagtgtct tttctatctt agcacgcctt ccctttattg gaatctttgt
    16741 gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt cacagggaat
    16801 ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct ttaaagaaat
    16861 cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac
    16921 ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag ttaagtccaa
    16981 gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt ttacccgagt
    17041 caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac tgattatcgt
    17101 tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa atttttaaaa
    17161 tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga
    17221 tgttggtggt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct aatctgacca
    17281 agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc
    17341 aggcagaact ttatagcttg gaaaagaatg aagatgctag cctaagcaag ttaaaagacg
    17401 atgggcgaat cacggaagaa caggttaaag cttataaaga atacaatgat aaaaatggag
    17461 gagcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct cttggttttg
    17521 ggtcttgtga gtatccttgc cttgggcttg tccggctctg ttcagtccac ttttgcggca
    17581 gtagaggaac agattttctt tatggaattt gaagaactct atcgggaaac ccaaaaacgc
    17641 agtgtagcca gtcagcaaaa gactagtctg aacttagatg ggcagacgat tagcaatggc
    17701 agtcaaaagt tgccagtccc taaaggaatt caggtcccat caggccaaag tattacattt
    17761 gaccgtgctg ggggcaattc gtccctggct aaggttgaat ttcagaccag taaaggagcg
    17821 attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga aacaaaaaat
    17881 tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca gcattgcgac
    17941 cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga
    18001 agaagtcttg agggtagcta agatggcctt gcagacgggg caaaatcagg taagcatcaa
    18061 cggagttgag attcaggtat tttctagtga aaaaggattg gaggtctacc atggttcaga
    18121 acagttgttg gcaatcaaag agccataagg tcaaggcttt taccttgtta gaatccctgc
    18181 ttgccctcat tgtcatcagt gggggattac tcctttttca agctatgagt cagctcctca
    18241 tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg tttgtggacc
    18301 aacttgaggt agaattagac cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca
    18361 tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa
    18421 ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca gctcagatta
    18481 cagaggacaa tcaactggtt cattttcgtt tccagtttca aaaaggctta gaaagggagt
    18541 tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta tgcagtcacc
    18601 atggcagcta tctttagtct tttgttgcaa ttttacttga accgacaagt cgcccactat
    18661 caagactatg ctttgaataa agaaaaattg gttgcttttg ccatggctaa acgaaccaag
    18721 gataaagccg agcaagaaag tggggaacag gtctttaatc taggtcaggt aagctatcaa
    18781 aacaagaaaa caagcttggt gacgactgtt cgtacgtcta agagccaata tgagtttctg
    18841 tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt agcgaccgat
    18901 tcaagcgaaa aagtggagaa gaaaaaatca gaagagaagc ctgaaaagaa agagaattcc
    18961 tagtcaattc aactataatg cgttgaatca agaatagtcc actatagttt ctagaaaatt
    19021 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
    19081 tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgattc
    19141 ccctaaaaat atcttcctcg caaacttggt atgtcaagca gccgagaaac agattgatct
    19201 tctatcagac aaagaaattt tagatttcgg tggtggcacg ggtctattag ccttgcccct
    19261 agcccctagc caagcaggct aagtcagtca ctcttgtaga catttctgag aaaatgttgg
    19321 agcaagctcg tttgaaagtg gagcagcaag caatcaagaa tatccagttt ttggagcaag
    19381 atttaccgaa aaatcccttg gagaaagagt ttgattgcct tgctgttagt cgggttcttc
    19441 atcatatgcc tgatttggat gcggctctct cactgtttca tcaacatttg aaggaagatg
    19501 ggaaactcat cattgctgat tttaccaaga cagaagctaa tcatcatgga tttgatttag
    19561 ctgaactgga aaacaagcta attgagcatg gtttttcatc tgtgcatagt cagattctct
    19621 atagcgctga agacctgttt caaggaaatc actcagaatt ctttttaaca gtagcccaaa
    19681 aatcactcgc ctagtcaggg agtgattttt ctataagaat ggaaaaaaga agggaaattt
    19741 gataagatag gaatatggat tttgaaaaaa ttgaacaagc ttatacctat ttactagaga
    19801 atgtccaagt catccaaagt gatttggcga ccaactttta tgacgccttg gtggagcaaa
    19861 acagcatcta tctggatggt gagactgagc tagaacaggt caaagacaac aatcagaccc
    19921 ttaagcgttt agcactacgc aaagaagaat ggctcaagac ctaccagttt ctcttgatga
    19981 aggctgggca aacagaaccc ttgcaggcca atcaccagtt tacaccggat gctattgctt
    20041 tacttttggt gtttattgtg gaagagctgt ttaaagagga ggaaattact atcctcgaaa
    20101 tgggttctgg gatgggaatt ttgggcgcta ctttcttgac ctcgcttact aaaaaggtgg
    20161 attatttggg aatggaagtg gatgatttgc tgattgattt ggcagctagc atggcagatg
    20221 taattggttt gcaggctggc tttgtccaag gagatgccgt tcgtccacaa atgctcaaag
    20281 aaagtgatgt ggtcatcagc gacttgcctg ttggctatta tcctgatgat gccgttgcgt
    20341 cgcgccatca agttgcttct agtcaagaac atacttacgc ccatcacttg ctcatggaac
    20401 aaggacttaa gtacctcaag tcagacggat acgctatttt tctagctccg agtgatttgt
    20461 tgaccagtcc tcaaagtaat ttgttgaaag tctggttgaa agaggaggca agtctggttg
    20521 ctatgattag tttacctgaa aatctctttg ctaatgctaa acaatctaag actattttta
    20581 tcctacagaa gaaaagtgaa atagcagtag agccttttgt ttatccactt gctagcttgc
    20641 aagatgcaag tgttttaatg aaatttaaag aaaattttca aaaatggact caaggtactg
    20701 aaatataaaa tagattttgt tataatagtt gaaaacgctt aaaaaggggt atcatgttat
    20761 gacaaaaaca attgcaatca atgcaggaag ttcaagtttg aaatggcaat tatacttaat
    20821 gccagaagaa aaagtattgg cgaaaggttt gattgaacgt atcggtttga aagattcaat
    20881 ttcaactgta aaatttgacg gccgttctga acaacaaatt ttggatattg aaaatcatac
    20941 acaagctgtt aaaattttat tggatgactt gattcgtttc gatattatca aggcttatga
    21001 cgagattaca ggtgttggac atcgtgttgt tgctggtgga gaatatttca aagaatcaac
    21061 agttgttgag ggagatgttt tagaaaaagt tgaagagttg agtttgttgg ctcctctaca
    21121 caacccggca aatgcagcag gtgttcgtgc cttcaaggaa ttgttgccag acattaccag
    21181 tgtagttgtt tttgatactt ccttccacac aagtatgcca gagaaagctt atcgctaccc
    21241 tctaccaaca aaatattaca cagaaaacaa ggttcgtaaa tacggtgctc atggtacaag
    21301 tcaccagttt gtagcaggag aagctgcaaa actcttggga cgtccattag aagacttgaa
    21361 gttaattacc tgtcatattg gtaacggagg ctcaattaca gctgtgaaag ccggcaaatc
    21421 tgtagacact tctatggggt tcactcctct tggtggtatt atgatgggaa cgcgtacagg
    21481 ggatattgat ccagctatca ttccttattt aatgcaatat acagaggatt ttaacacacc
    21541 agaagatatc agtcgtgttc ttaaccgtga atcaggtctt ttgggagttt ctgctaattc
    21601 tagcgatatg cgcgatatag aagcagctgt agcagaaggg aatcacgagg ctagcttggc
    21661 ttatgaaatg tatgttgacc gtatccaaaa acatatcggt cagtaccttg cagtgctaaa
    21721 tggagcagat gccattgttt tcacagcagg tgtcggtgaa aatgcagaga gtttccgtcg
    21781 tgatgtaatc tcagggattt cgtggtttgg ttgtgatgtt gatgatgaaa agaatgtctt
    21841 tggcgttaca ggagacatct caacagaggc agctaaaatc cgtgtcttgg ttattccaac
    21901 agatgaagaa ttagtcattg cccgtgacgt tgaacgcttg aaaaaataag tgaaactaaa
    21961 aaaatattca atacaaggag ttgggaaagt tatttttcca gcttcttttt ctgatgaaat
    22021 tgtccaaaac cttgctatga ttggcttttt tgaaaaatat ggtataatag tagtaattta
    22081 atagatggag ttgagttttg aagaaaaact ttcgtgtaaa aagagagaaa gattttaagg
    22141 cgattttcaa ggaggggaca agttttgcta atcgcaaatt tgtggtctac caattagaaa
    22201 accagaaaaa ccattttcga gtaggtctat cagttagcaa aaaactgggg aatgccgtca
    22261 ctagaaatca aattaagcga cggattcggc atattatcca gaatgcaaaa gggagtctgg
    22321 tagaagatgt cgactttgtt gtcattgctc gaaaaggagt cgaaaccttg ggatacgcag
    22381 agatggagaa aaatctactc catgtattaa aattatcaaa gatttaccgg gaaggaaatg
    22441 ggagtgaaaa agaaactaaa gttgactagt ttgctaggac tgtctctgtt aatcatgaca
    22501 gcctgtgcga ctaatggggt aactagcgat attacagccg aatcggctga tttttggagt
    22561 aaattggttt acttctttgc ggaaatcatt cgctttttat cgtttgatat tagtatcgga
    22621 gtggggatta ttctctttac ggtcttgatt cgtacagtcc tcttgccagt ctttcaggtg
    22681 caaatggtgg cttctaggaa aatgcaggaa gctcagccac gcattaaggc gcttcgagaa
    22741 caatatccag gtcgagatat ggaaagcaga accaaactag agcaggaaat gcgtaaagta
    22801 tttaaagaaa tgggtgtcag acagtcagac tctctttggc cgattttgat tcagatgccg
    22861 gttattttgg ccttgttcca agccctatca agagttgact ttttaaagac aggtcatttc
    22921 ttatggatta accttggtag tgtggataca acccttgttc ttccgatttt agcagcagta
    22981 ttcacctttt taagtacttg gttgtccaac aaagctttgt ctgagcgaaa tggcgctacg
    23041 actgcgatga tgtatgggat tccagtcttg atttttatct ttgcagttta tgcgccaggt
    23101 ggagtcgccc tatactggac agtgtctaat gcttatcaag tcttgcaaac ctatttcttg
    23161 aataatccat tcaagattat cgcagagcgc gaggccgtag tacaggcaca aaaagatttg
    23221 gaaaatagaa aaagaaaagc caagaaaaag gctcagaaaa cgaaataaat aaggaggaat
    23281 ctggtagtgg tagtatttac aggttcaact gttgaagaag caatccagaa aggattgaaa
    23341 gaattagata ttccaagaat gaaggctcat atcaaagtca tttctaggga gaaaaaaggc
    23401 tttcttggtc tatttggtaa aaaaccagcc caagtggata ttgaagcgat tagtgaaacg
    23461 actgttgtca aagcaaatca acaggtagta aaaggcgttc cgaaaaaaat caatgatttg
    23521 aacgagcctg tgaagacggt tagtgaagaa accgttgacc ttggtcatgt ggttgatgct
    23581 attaaaaaaa tagaggaaga aggtcaaggt atttctgatg aagtcaaggc tgaaatctta
    23641 aaacatgaaa gacatgccag cactatctta gaagaaactg gtcacattga gattttaaat
    23701 gaacttcaaa tcgaggaagc gatgagggaa gaagcaggcg ctgatgacct tgaaactgag
    23761 caagaccaag ctgaaagtca agaactagaa gacttgggct tgaaagttga aacgaacttt
    23821 gatattgaac aagtagctac ggaagtaatg gcttatgttc aaacgattat tgatgacatg
    23881 gatgttgagg ctacactttc aaatgattat aaccgtcgta gcatcaattt gcaaattgat
    23941 actaacgaac caggtcgtat tatcggctac catggtaaag tcttgaaggc cttgcaactg
    24001 ttggctcaaa attatcttta caactgctat tcaagaacct tctatatcac tattaatgtc
    24061 aatgattatg ttgaacaccg tgcagaagtc ttgcagacct atgcgcaaaa attggcgatt
    24121 cgtgttttag aagaaggacg tagtcatcaa acagatccaa tgtcgaatag tgaacgcaag
    24181 attatccacc gtattatttc acgtatggat ggcgtgacca gttactctga aggtgatgag
    24241 ccaaatcgct atgtcgttgt agatacagaa taagtaaaat cagggttgac ctgatttttt
    24301 gctagctaga ggaggttaaa ctgatgttga ataagataag agactatcta gacttttctg
    24361 gtttgcagta ccgtagtcct gataaagcgg gagcagagcg agagaagatg ctggcattcc
    24421 gccacaaagg tcaagaggca cgaaaggctt ttacagaatt ggctaaagtc tttcaggcaa
    24481 atcatccaga atggcaactc caacagacta gccagtggat gaatcaggcc cagcgtttgc
    24541 gaccacattt ttgggtttat ctacaacgtg aggggaaagt gacagaacct atgatagcct
    24601 tgcgtttgta tggaaatcct tctgattttg gtatttcctt agaggtcagt ttcatcgaac
    24661 ataagaagga tgagcaaact ttagagaaac aggccaagat tttagacatt ccaaccgtta
    24721 aaggaattta ttatctagcc tactctgatg gccaaagtca acggtgggag gcgaacgaag
    24781 aaaagcgtcg tactttacgc gagaaggtga gaagtcaaga agttcgaaaa gttttagtga
    24841 aggtagatgt tcctatgaca gaaaattcgt ctgaagaaga aatcgtagaa ggcttattga
    24901 agtcttattc taaaattctt ccctattatc tagctacgag aaaataagat aatttgtaaa
    24961 acatcataaa tcatacagtc caagagtgaa cagtccgctg tgtaattctt ggtctttttg
    25021 tttgcgcttt cgcattatat aataaactta caaaaacaat tcaaaaggag aacaattatg
    25081 gaagtcgttt caagtgttct aaattggttt tctagcaata ttttgcagaa tcccgcattt
    25141 ttcgtaggtt tattggtgtt gataggatat gcacttttga aaaaacctgc ccatgacgtt
    25201 ttttcagggt ttgttaaagc aacagtaggg tatatgttgc ttaacgtggg tgctggtggt
    25261 ttggttacaa cctttcgtcc aatcttagca gctcttaact acaaattcca aattggtgca
    25321 gcggttatcg acccttactt tggacttgct gcagcaaaca acaaaattgc agcagagttt
    25381 ccagattttg ttggaactgc aactacagct ctattgattg gttttggaat aaatatcttg
    25441 ctcgtagctc ttcgaaagat tacgaaggta agaaccctct ttattactgg tcacatcatg
    25501 gtacaacaag ctgcaacagt atctcttatg gttctattct tagtaccaca attgcgcaat
    25561 gcttacggta cagcagcgat tggtatcatc tgtggacttt actgggcagt tagttcaaat
    25621 atgactgttg aggcaactca acgcttgact ggtggtggcg gatttgcgat tggtcaccaa
    25681 cagcaatttg caatctggtt tgtagataaa gtagcaggac gctttggtaa gaaagaagaa
    25741 agtttagaca atcttaaatt acctaagttc ctctcaatct tccacgatac agttgttgca
    25801 tctgctacct tgatgctcgt attcttcgga gccattcttt taatcttggg tccagacatt
    25861 atgtctaata aagaagtcat cacttcagga actctattca atcctgctaa acaagatttc
    25921 tttatgtaca ttatccaaac agcctttacc ttctcagttt acttgttcgt tttgatgcaa
    25981 ggtgtccgaa tgttcgtatc tgagttgaca aacgctttcc aaggtatttc aaacaaattg
    26041 ttgccaggtt cattcccagc ggttgacgtt gcagcttctt atggatttgg ttctccaaat
    26101 gctgtcttgt caggatttac ctttggtttg attggtcaat tgattacaat tgtcttgctc
    26161 atcgtcttta aaaatccgat tcttattatt acaggatttg taccagtgtt ctttgacaat
    26221 gcagccattg cggtctacgc tgataaacgc ggcggatgga aagcggctgt tatcctttcc
    26281 tttatatcag gtgtccttca agttgctcta ggagctcttt gtgtggccct tctcgatttg
    26341 gcatcttatg gtggctacca tggaaatatc gactttgaat tcccatggct tggatttgga
    26401 tatatcttca aataccttgg tattgttggt tatgtacttg tgtgtctctt cttgcttgtt
    26461 attcctcaac ttcaatttgc caaagcaaaa gataaagaga aatattacaa cggtgaagtt
    26521 caagaagaag cttagtatct agaaaaggag aaataaaatg gttaaagtat tagcagcgtg
    26581 cggaaatgga atgggttcat caatggttat caagatgaag gttgaaaatg ctctccgtaa
    26641 gcttaatcaa acagatttta cagtcaattc atgcagtgtc ggtgaagcta aaggtttagc
    26701 agtaggatat gacatcgtaa tcgcttctct tcatttgatt caagaattgg aagggcgaac
    26761 taatgggaag ttaattggac ttgataactt gatggatgat aaagaaatca ccgaaaaact
    26821 cagtcaagca ctacagtaaa aggttggagg gggctggaca gaaactgaga gttatcgttt
    26881 ctgtccttct ccctctttaa ataaaggagg cagatatgaa tttaaaacaa gctttaattg
    26941 acaacgactc gatccgacta ggtttagagg ctaacgattg gaaagaagca gtcaaggtag
    27001 cagtagatcc cttgattgaa agtggggcaa ttttgccaga gtattacgat gctatcattg
    27061 aatcgactga agagtatggg ccttactata tcttgatgcc aggtatggct atgccccacg
    27121 ctagacctga agctggtgtg caaagtgatg ccttttcatt aattacctta caaaatcctg
    27181 ttgtattttc agatgggaaa gaggtatctg ttttgttggc actagcagca acaagttcaa
    27241 aaattcacac aagtgtagcc attccacaaa ttattgccct gtttgaatta gaagattcta
    27301 ttgcacgttt acaggcttgc cagactaaag aagatgtctt ggctatgatt gaagaatcta
    27361 aggatagccc ttatctcgaa ggattggatt tggaaagtta gaaagaggaa taaagaaatg
    27421 acaaaaagaa tacctaattt acaagttgca ttagaccatt cagacttgca aggagcgatt
    27481 aaagcagctg tttctgttgg tcaggaagta gatattatcg aagctggaac tgtttgcttg
    27541 cttcaagttg gaagtgaact ggctgaagtc ttgcgtagcc ttttcccaga taagattatt
    27601 gtggcagaca caaaatgtgc tgatgctggt ggaacagttg ctaaaaataa tgcggttcgt
    27661 ggagcagact ggatgacttg tatctgttgt gcaaccatcc ctactatgga agcagctcta
    27721 aaggctatca agactgaacg aggagaacga ggcgaaatcc agatcgagct ttatggcgat
    27781 tggacttttg aacaagctca gctttggcta gatgcaggta tctcacaagc tatttatcac
    27841 caatctcgtg atgctcttct tgctggtgaa acttggggtg aaaaagacct taataaggtt
    27901 aaaaaactca ttgacatggg cttccgtgta tctgtaacag gtggtctaga tgtagatact
    27961 ctcaaactct ttgaaggtgt tgatgtcttt acctttatcg caggtcgtgg aattacagag
    28021 gctgtggatc cagcaggagc agcgcgtgcc ttcaaggatg aaatcaaacg aatttggggg
    28081 taaatcatgg tacgtccaat tggaatttat gaaaaggcaa ccccaacaca ctttacttgg
    28141 ctagaacgtt taaattttgc caaggagtta ggctttgatt ttatcgagat gtctattgac
    28201 gaacgtgacg agcgtttagc aagacttgac tggagtaagg aagaacgctt ggaagttgtc
    28261 aaagaaatct atgaaactgg tgttcgtatt ccttctatct gtttttcagg ccatcgtcgc
    28321 tacccattgg gatcaaaaga ttcagttcta gaggaaaaat ctctagaact catgaaaaaa
    28381 tgtatcgaat tagctcaaga cttgggagtt cgtacgattc aattagctgg ttacgatgtt
    28441 tactatgagg aaaagtcacc ccagacacgc caacgtttta tcaaaaattt gagaaaagcc
    28501 tgtgactggg ctgaagaagc tcaggtggta cttgctattg aaattatgga tgatcctttc
    28561 atcagtagca tcgaaaaata tttggctata gaaaaagaga ttgactctcc cttcctcttt
    28621 gtatatccag atattggtaa tgtgtctgca tggcataatg atatctatag tgagttttat
    28681 cttggtcatc atgccatcgc agctctccat ctcaaggata cttatgcagt gacagaaagt
    28741 tcaaagggcc agttccgaga tgtacctttc gggcaaggtt gtgtcaaatg ggaagaagct
    28801 ttcgatattt taaaggaaac caattataat ggacctttcc taatcgaaat gtggtctgaa
    28861 aattgtgaaa cagtagaaga aacacgcgca gccattcaag aggcgcaagc ttttctctat
    28921 ccactcatta agaaagcagg tttgatgtaa gatgaatcaa gtaatcaatg ctatgcgtaa
    28981 acgagtctgt gatgccaatc aatcattgcc aaaacatgga cttgtcaaat ttacctgggg
    29041 gaatgtatct gaagtcaatc gcgaactcgg tgtcattgtt atcaaaccat caggcgtgga
    29101 ttatgacgaa ttgacacctg aaaacatggt agtgactgat ctagatggta agatcctaga
    29161 aggggattta agaccatctt ccgacctccc aactcatgtg caattatata gggcttggtc
    29221 agaaattggt agtgtggttc acacccattc gacagaagct gttggttggg ctcaggcagg
    29281 tcgtgatatt cctttctacg gaacaaccca tgcagattat ttctacggtt caatcccttg
    29341 cgcccgtagt ttgaccaagg acgaagtaga agtggcctat gaaaaagata ctggcctggt
    29401 tatcgtagaa gagtttgaac atcgcggact taacccggtt gaagtaccag gaattgttgt
    29461 acgcaatcac ggtccattca cctggggcaa aaatccagag aatgctgttt atcactctgt
    29521 cgtactagag gaagtatcaa agatgaatcg ctttacagaa caaatcaatc caagagttgg
    29581 acctgctccc cagtacatac tagaaaaaca ctaccaacgt aaacatggac caaatgctta
    29641 ttatggtcaa aagtaagaac gatgaaggag gagaaaaaga taaatttagc tcctcttttt
    29701 acatttgatt tttattgaga gtaaagttgg agttgaagta attttaaaag attttttaga
    29761 aatagcgctt gatatatata tggtaaaata aaaagaattg ctgtgatatc aatagatttg
    29821 ggggattttt taatatggta ctggataagg caagttgtga tttgcttcaa tatttgatgg
    29881 atcaagaaac gtccaaaacg attatggcga tttcgaaaga tttgaaagag tcaagaagga
    29941 aaatttatta tcacattgac aaaatcaatg ctgctctggg tgacgaggcg cttcacatca
    30001 ttagtattcc acgaattggt attcacttaa cggaagagca gagagatgct tgttgtaaac
    30061 tattatcgga agtagattcg tacgattata tcatgagtgc gcatgaacgt atgatgataa
    30121 tgttactatg gataggtatt tctaaagaac gtattacgat tgaaaaattg atagagttaa
    30181 cagaggtatc taggaatact gttctcaatg atttgaatag tattcgttat caactaactt
    30241 tggaacaata tcaggtgatc ttgcaagtga gcaagtcaca gggataccac cttcatgccc
    30301 accctcttaa taaaattcag tatcttcaat cgcttctata tcatattttt atggaagaaa
    30361 atgccacttt tgtatctatt ttagaagata agatgaaaga gaggttagat gatgagtgtt
    30421 tgctttctgt tgaaatgaac caatttttta aggaacaggt tcctttagtt gaacaagatt
    30481 tagggaagaa aataaaccat catgaaataa cttttatgtt gcaggttcta ccttatttgc
    30541 tgttaagctg tcataatgtt gaacagtatc aagaaagaca tcaggatata gagaaagaat
    30601 tttctttgat aagaaaaaga atagagtatc aggtgtctaa gaaattagga gaacggttgt
    30661 ttcaaaagtt tgaaatttct ttgtcaggac ttgaagtttc tcttgtagct gttctcctcc
    30721 tctcctatcg taaagatttg gatattcatg cagaaagtga tgattttcgg caattaaaac
    30781 ttgctttaga agaatttatc tggtattttg aatcacaaat ccgaatggag attgagaaca
    30841 aggatgattt gttacgaaat ttgatgatcc actgtaaagc cttgttattt agaaagactt
    30901 acggtatttt ttctaaaaat cctctaacaa aacaaattcg atccaagtat ggagaattat
    30961 ttttagtcac tagaaaatct gcggaaattt tagaaggagc atggtttatt cggctaacag
    31021 acgatgatat tgcctatttg acgattcata ttggaggatt tttaaagtat acaccatcgt
    31081 ctcaaaaaaa tatgaaaaaa gtttatctcg tttgtgatga aggtgttgcg gtttcgagac
    31141 ttttgctgaa acaatgcaaa ctttattttc caaatgagca aattgacact gtatttacaa
    31201 cagaacaatt taagagtgtg gaagatattg cacaagttga tgtagtgatt actactaatg
    31261 atgatttgga tagcagattt ccgattttaa gggttaatcc tatccttgaa gcagaagata
    31321 ttttgaaaat gctagactat cttaaacaca atatatttcg taatgagagc aaaagtttca
    31381 gtgaaaatct ttctagtctt atttcgtctt atattgtaga tagcaagttg gctagtaagt
    31441 tccaagaaga ggttcaaaca cttataaatc aagaaatagt agttcaagct tttttggaag
    31501 atatttgaag gacagtccaa tgatgaacac aaacctgtgt ttttcttggt cttttttagt
    31561 gttttgaagg gtggtatact aatctcaaag ataacaatta tatccaaagg aggcaacata
    31621 tgccaaacgt caaagaaatt acaagagagt catggatttt agccactttc ccagagtggg
    31681 gaacatggtt gaacgaagaa atcgaagaag aagtcgtacc tgaaggcaac tttgccatgt
    31741 ggtggctagg caactgtggt acttggatta agacaccagc tggtgctaac gttgtcatgg
    31801 acctttggtc aaaccgtgga aaatcaacca aaaaagtgaa agatatggtt cgtgggcacc
    31861 aaatggcaaa tatggcaggt gttcgtaagt tgcaaccaaa cttgcgtgtt cagccaatgg
    31921 ttatcgatcc atttgctatc aacgaactag actattactt agtttcacac ttccacagtg
    31981 atcatatcga cccatacaca gctgcagcaa ttctcaacaa tcctaagtta gagcatgtta
    32041 agtttatcgg tccttaccac tgtggacgaa tctgggaagg atggggtgtt ccaaaagaac
    32101 gtatcatcgt tgttaaacca ggtgacatta tcgaattaaa agatatgaag attcatgcag
    32161 tagaatcatt tgaccgtact tgcttggtaa ctctcccagt gaacggtgct gatgagacag
    32221 gcggtgaact tgctggcttg gctgttacag atgaagaaat ggctcaaaag gctgttaact
    32281 atatctttga aacaccaggt ggaaccatct atcatggtgc agattctcac ttctcaaact
    32341 attttgcaaa acatggtaaa gactttaaaa ttgatgttgc tttgaataac tatggtgaaa
    32401 atccggtagg tatccaagac aaaatgacat ctatcgacct tcttcgtatg gcagaaaatc
    32461 tgcgtaccaa agtcattatc ccagttcact atgatatctg gtctaacttc atggcttcta
    32521 ctaatgagat tctagaactt tggaaaatgc gaaaagatcg cttgcaatac gatttccatc
    32581 catttatctg ggaagttggc ggtaagtaca cttatcctca agatcaacac ttagtagaat
    32641 accatcatcc acgtggtttt gatgattgtt ttgaacaaga ctctaacatt caatttaaag
    32701 ctttgctata atataaaaat attctttgga ggttatccgt atgttgcatg atacggataa
    32761 ttttcatata atagtaaaat agagtgtgtg attcaataat cacctcaaat agaaaggaaa
    32821 ttctatgtca aatctatctg ttaatgcaat tcgttttcta ggtattgacg ccattaataa
    32881 agccaactca ggtcatccag gtgtggttat gggagcggct ccgatggctt acagcctctt
    32941 tacaaaacaa cttcatatca atccagctca accaaactgg attaaccgcg accgctttat
    33001 tctttcagca ggtcatggtt caatgctcct ttatgctctt cttcaccttt ctggttttga
    33061 agatgtcagc atggatgaga ttaagagttt ccgtcaatgg ggttcaaaaa caccaggtca
    33121 cccagaattt ggtcatacgg cagggattga tgctacgaca ggtcctctag ggcaaggtat
    33181 ttcaactgct actggttttg cccaagcaga acgtttcttg gcagccaaat ataaccgtga
    33241 aggctacaat atctttgacc actatactta cgttatctgt ggagacggag acttgatgga
    33301 aggtgtctca agcgaggcag cttcatacgc aggtttgcaa aaacttgata agttggttgt
    33361 tctttatgat tcaaatgata tcaacttgga tggtgagaca aaggattcct ttacagaaag
    33421 tgttcgtgac cgttacaatg cctacggttg gcatactgcc ttggttgaaa atggaacaga
    33481 cttggaagcc atccatgctg ctatcgaaac agcaaaagct tcaggcaagc catctttgat
    33541 tgaagtgaag acggttattg gatatggttc tccaaacaaa caaggaacta atgctgtaca
    33601 cggcgcccct cttggagcag atgaaactgc atcaactcgt caagccctcg gttgggacta
    33661 cgaaccattt gaaattccag aacaagtata tgctgatttc aaagaacatg ttgcagaccg
    33721 tggcgcatca gcttatcaag cttggactaa attagttgca gattataaag aagctcatcc
    33781 agaactggct gcagaagtag aagccatcat cgacggacgt gatccagtcg aagtgactcc
    33841 agcagacttc ccagctttag aaaatggttt ttctcaagca actcgtaatt caagtcaaga
    33901 tgccttgaat gttgtggcag ctaaattacc aaccttccta ggtggatcag ctgaccttgc
    33961 tcactcaaac atgacttata tcaagacgga tggacttcaa gacgatgcca atcgcttgaa
    34021 tcgtaacatt cagtttggtg ttcgtgaatt tgcaatggga acaatcttga acgggatggc
    34081 ccttcatggt ggacttcgtg tatacggtgg aactttcttc gtcttctctg actatgtgaa
    34141 ggcagctgtc cgcttgtcag ccttacaagg acttcctgtg acttatgtct ttacccatga
    34201 ttcaatcgca gttggggaag atggtccgac tcacgaacca gttgagcatt tagcaggtct
    34261 tcgtgctatg ccaaatctaa atgttttccg tccagcagat gcgcgtgaaa ctcaagcagc
    34321 ttggtacctt gcagtgacaa gtgagaaaac accaactgcc cttgtcttga cacgtcaaaa
    34381 cttgactgtt gaagatggaa cagacttcga caaggttgct aaaggtgctt atgttgtata
    34441 tgaaaatgca gccgactttg ataccatctt gattgcgaca ggttcagagg ttaatcttgc
    34501 tgtctcagct gctaaagaat tggctagtca aggcgaaaaa atccgcgtag tcagcatgcc
    34561 atctacagat gtctttgata aacaagatgc agcttacaag gaagagattc ttccaaatgc
    34621 agtccgccgt cgtgttgcag tcgaaatggg tgcaagtcaa aactggtaca aatatgttgg
    34681 tctcgatggt gccgttctag gtattgatac cttcggagcc tctgccccag caccaaaagt
    34741 attggcagaa tatggcttta ctgtagaaaa tcttgtaaaa attgttcgaa acttgaaata
    34801 atcctaaaaa tcagggcgta agctctggtt tttcttacca gaaaagtaag gtacaatctt
    34861 gtaaaagtag ctgaaatttg atatagtagt cctatgtaaa agacaaagga gaaaacaatg
    34921 aatccaaata ttactttttt aatcatgctt gtaggtatga tggccttgat gttctttatg
    34981 caacgttctc aaaagaaaca agctcaaaag cgtatggaaa gcttaaataa actacaaaaa
    35041 ggctatgaag tgattacaat cggtggactt tatggaacag tcgatgaagt agatacggag
    35101 aagggaacaa tagttcttga cgtagatggg gtttacttga cttttgaact agctgctatc
    35161 aagacagtat taccgctgaa agaaacagct tcactagaag gcgcaattga aaaataagac
    35221 gggatcacga actcccgttt ttctataaaa gaaaggaaat gggatgaaaa aattagtctt
    35281 tgtctgtctg ggaaatattt gccgtagccc tatggccgag tttgttatga aatcaatgac
    35341 agataactac gaaatccaaa gtcgagcaac ttcctcttgg gaacatggca atccgattca
    35401 taaggggact cagggaattt ttcaagagta tgagattcct tatgacaaga acaagacatc
    35461 gcttcagatt agtaaggaag attttgaagc ctttgattat attatcggaa tggacgcttc
    35521 aaatgtttcc gacttacgtc agatgtgtcc agtagactgt caagataaga tttactcatt
    35581 ttcatctgaa agtgttccag acccttggta tacaggagat tttgaagaaa cctatcgacg
    35641 tgttcaagag ggctgtcaag cttggttaga acgtttagaa aaggagagtt aaggtggaaa
    35701 atcttacgaa tttttacgaa aagtatcgtg tttatctgac tcgtccacgt ttagagattt
    35761 tgacagtagt taccattgtt ttctgtgctg tactcgtctt ttttctaaat attccaggaa
    35821 aaggtgtctt aaaactcgat aatggaacga ttgtttatga tggcagtctt gtccgcggta
    35881 aaatgaatgg ccaaggtacc attaccttcc aaaatggaga ccaatataca ggtggcttca
    35941 acaatggagc cttcaacgga aaaggtacct ttcaatctaa agaaggctgg acctacgaag
    36001 gtgattttgt aaatggtcag gctgaaggaa aagggaaact aacaacagaa caagaagtcg
    36061 tttatgaagg aacttttaaa caaggcgttt ttcaacaaaa ataaagcctc cttatcaaag
    36121 gaggtattat tagaatcgca aggtaagcgc ttacctgcaa atccctttct ttccaaatcc
    36181 ctcttccaac caagtttgtg aaataaaaaa tatttgaaat aaatttcaca aacttcaaag
    36241 ataaaacttg ataagaaaag aaaatgagaa aagtttcaca agagtttaaa aattctttgt
    36301 gaaatgttta tgaaactgtt tacaaagttg aaataatacg ttataataaa cttgtgaaaa
    36361 aattaacaaa ggatatcgat ccttgaaagc tatggaggaa aatatggctg ataaaaaaac
    36421 tgtgacacca gaggaaaaga aactcgttgc tgaaaaacac gtagatgagt tggttcaaaa
    36481 agctctagtt gcccttgaag aaatgcgtaa attggatcaa gagcaagttg actacatcgt
    36541 tgccaaagca tcagtagcag ctttggatgc ccacggagaa ttggctttac atgcctttga
    36601 agaaacagga cgtggtgtat ttgaagataa agcaactaag aacttgtttg cctgtgaaca
    36661 cgtagtaaac aacatgcgtc acactaagac agttggcgtt atcgaagaag acgatgtaac
    36721 aggattgact cttattgctg aaccagttgg tgttgtttgt ggtattactc caacaacaaa
    36781 cccaacatca acagcaatct ttaaatcatt gatttcattg aagacacgta acccaatcgt
    36841 ctttgccttc catccatcag cacaagaatc atctgctcat gcagctcgta tcgtccgcga
    36901 tgcagctatc gcagctggtg ctcctgaaaa ctgtgtgcaa tggattactc aaccatctat
    36961 ggaagcaaca agtgccctta tgaaccacga aggtgttgcg acaatccttg caacaggtgg
    37021 taatgccatg gttaaggcgg cttattcatg tggtaaacca gctcttgggg taggtgccgg
    37081 aaacgttcca gcttatgttg aaaaatcagc aaacattcgt caagcagcac acgatatcgt
    37141 catgtctaaa tcatttgata acggtatggt ctgtgcatct gaacaagcag ttatcattga
    37201 taaagaaatt tacgatgaat ttgtagcaga gttcaaatct taccacactt actttgtaaa
    37261 caaaaaagaa aaagctcttc ttgaagagtt ctgcttcggc gtcaaagcaa acagcaaaaa
    37321 ctgtgctggt gcaaaattga acgctgacat cgttggtaaa ccagcaactt ggattgcaga
    37381 acaagcagga tttacagttc cagaaggaac aaacattctt gctgcagaat gtaaagaagt
    37441 tggcgaaaat gagccattga ctcgtgaaaa attgtcacca gttattgcag ttttgaaatc
    37501 tgaaagccgt gaagatggta ttactaaggc tcgtcaaatg gttgaattta acggtcttgg
    37561 acactcagca gctatccaca cagctgacga agaattgact aaagaatttg gtaaagctgt
    37621 taaagctatt cgtgttatct gtaactcacc ttctactttt ggtggtatcg gggacgttta
    37681 caatgccttc ttgccatcat tgacacttgg atgtggttct tacggacgca actcagttgg
    37741 ggataacgtt agtgccatta acctcttgaa tatcaaaaaa gtcggaagac ggagaaataa
    37801 catgcaatgg atgaaacttc cttcaaaaac atactttgaa cgtgattcaa ttcaatacct
    37861 tcaaaaatgt cgtgacgttg aacgtgtcat gatcgttact gaccatgcca tggtagagct
    37921 tggtttcctt gatcgtatca tcgaacaact ggaccttcgt cgcaataagg ttgtttacca
    37981 aatctttgcg gatgtagaac cggatccaga tatcacaact gtaaaccgtg gtactgagat
    38041 tatgcgtgcc ttcaaaccag ataccatcat cgcactcggt agtgggtctc caatggatgc
    38101 tgccaaagta atgtggctct tctacgagca accagaagtg gacttccgtg accttgtcca
    38161 aaaattcatg gatatccgta aacgtgcctt caagttccca ttgcttggta agaagactaa
    38221 attcatcgcg attccaacta catctggtac aggatctgaa gtaacaccat ttgccgttat
    38281 ctctgataaa gcaaacaacc gtaaataccc aatcgctgac tactcattga caccaactgt
    38341 ggcaatcgta gatcctgctt tggtattgac agttccagga tttgttgctg ctgatactgg
    38401 tatgggcgta ttgactcacg cgacagaagc atacgtatca caaatggcta gtgactacac
    38461 tgatggttta gcacttcaag ccattaaatt ggtctttgaa aatctcgaaa gctcagttaa
    38521 gaatgcagac ttccactcac gtgagaaaat gcataacgct tcaacaatcg ctggtatggc
    38581 ctttgccaat gccttcctag gtatttctca ctcaatggcc cataagattg gtgcgcaatt
    38641 ccacacaatc cacggtcgta caaatgctat cttgcttcca tacgttatcc gttacaacgg
    38701 tacacgtcca gctaagacag caacatggcc taagtacaac tactaccgtg cagatgaaaa
    38761 ataccaagat atcgcacgca tgcttggact tccagcttct actccagaag aaggggttga
    38821 atcttacgca aaagctgtct acgaactcgg tgaacgtatt gggatccaaa tgaattttag
    38881 agaccaagga attgacgaaa aagaatggaa agaacattct cgtgaattag ccttcctggc
    38941 ttatgaagac caatgttcac cagctaaccc acgtcttcca atggtagacc atatgcaaga
    39001 aatcatcgaa gatgcatact atggctacaa agaaagacca ggacgccgta aataattgtt
    39061 tatcagtcta gaagcaagac aaaaactcaa tttgagggaa agatccagta atttttctat
    39121 gataaaaggc atcctatcaa ggtttttgaa cacctgatag gatgcctttt tatgatattg
    39181 aggccttttt gccctttttg aaaaactaga atagaaacaa aatatataat agattgaaac
    39241 tggaatagta cacttctgct tctaaaacat tgttagaatt
//
LOCUS       DAVGJJ010000009        34352 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000009 DAVGJJ010000000
VERSION     DAVGJJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.009"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            510..962
                     /locus_tag="VVE35_000437"
     CDS             510..962
                     /locus_tag="VVE35_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU3060688.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGD
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGEVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            981..2318
                     /locus_tag="VVE35_000438"
     CDS             981..2318
                     /locus_tag="VVE35_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU3060689.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2397..3281
                     /locus_tag="VVE35_000439"
     CDS             2397..3281
                     /locus_tag="VVE35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3060690.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3281..4114
                     /locus_tag="VVE35_000440"
     CDS             3281..4114
                     /locus_tag="VVE35_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3060691.1"
                     /translation="MKKKSGIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4132..6225
                     /gene="nanB"
                     /locus_tag="VVE35_000441"
     CDS             4132..6225
                     /gene="nanB"
                     /locus_tag="VVE35_000441"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU3060692.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLFISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6237..7340
                     /locus_tag="VVE35_000442"
     CDS             6237..7340
                     /locus_tag="VVE35_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU3060693.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7513..8211
                     /locus_tag="VVE35_000443"
     CDS             7513..8211
                     /locus_tag="VVE35_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU3060694.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8230..9747
                     /locus_tag="VVE35_000444"
     CDS             8230..9747
                     /locus_tag="VVE35_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU3060695.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9872..11200
                     /locus_tag="VVE35_000445"
     CDS             9872..11200
                     /locus_tag="VVE35_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3060696.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11453..12319
                     /locus_tag="VVE35_000446"
     CDS             11453..12319
                     /locus_tag="VVE35_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3060697.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12335..13174
                     /locus_tag="VVE35_000447"
     CDS             12335..13174
                     /locus_tag="VVE35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3060698.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13447..13899
                     /locus_tag="VVE35_000448"
     CDS             13447..13899
                     /locus_tag="VVE35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU3060699.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14041..14349
                     /locus_tag="VVE35_000449"
     CDS             14041..14349
                     /locus_tag="VVE35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060700.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKIPNNGRINF
                     "
     gene            14633..15277
                     /locus_tag="VVE35_000450"
     CDS             14633..15277
                     /locus_tag="VVE35_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU3060701.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15446..16363
                     /locus_tag="VVE35_000451"
     CDS             15446..16363
                     /locus_tag="VVE35_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU3060702.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16381..17265
                     /locus_tag="VVE35_000452"
     CDS             16381..17265
                     /locus_tag="VVE35_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3060703.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17281..18132)
                     /locus_tag="VVE35_000453"
     CDS             complement(17281..18132)
                     /locus_tag="VVE35_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU3060704.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18332..20389
                     /gene="pbp2b"
                     /locus_tag="VVE35_000454"
     CDS             18332..20389
                     /gene="pbp2b"
                     /locus_tag="VVE35_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU3060705.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20400..20996
                     /gene="recR"
                     /locus_tag="VVE35_000455"
     CDS             20400..20996
                     /gene="recR"
                     /locus_tag="VVE35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU3060706.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21172..22215
                     /locus_tag="VVE35_000456"
     CDS             21172..22215
                     /locus_tag="VVE35_000456"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU3060707.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISSPELIERLVDL
                     AKESFDKREAHLI"
     gene            22299..23672
                     /locus_tag="VVE35_000457"
     CDS             22299..23672
                     /locus_tag="VVE35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU3060708.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGVHDQILLKGSNSMNLAKLVE
                     SLENETK"
     gene            23659..24270
                     /locus_tag="VVE35_000458"
     CDS             23659..24270
                     /locus_tag="VVE35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3060709.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIAALQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24361..25065
                     /locus_tag="VVE35_000459"
     CDS             24361..25065
                     /locus_tag="VVE35_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU3060710.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25284..26657
                     /gene="ftsA"
                     /locus_tag="VVE35_000460"
     CDS             25284..26657
                     /gene="ftsA"
                     /locus_tag="VVE35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU3060711.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKSGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26674..27933
                     /gene="ftsZ"
                     /locus_tag="VVE35_000461"
     CDS             26674..27933
                     /gene="ftsZ"
                     /locus_tag="VVE35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU3060712.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27938..28609
                     /locus_tag="VVE35_000462"
     CDS             27938..28609
                     /locus_tag="VVE35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU3060713.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28619..29158
                     /locus_tag="VVE35_000463"
     CDS             28619..29158
                     /locus_tag="VVE35_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU3060714.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29158..29421
                     /locus_tag="VVE35_000464"
     CDS             29158..29421
                     /locus_tag="VVE35_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU3060715.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29418..30203
                     /locus_tag="VVE35_000465"
     CDS             29418..30203
                     /locus_tag="VVE35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU3060716.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30212..31000
                     /gene="divIVA"
                     /locus_tag="VVE35_000466"
     CDS             30212..31000
                     /gene="divIVA"
                     /locus_tag="VVE35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU3060717.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31348..34140
                     /locus_tag="VVE35_000467"
     CDS             31348..34140
                     /locus_tag="VVE35_000467"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU3060718.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10558 a         6086 c         7474 g        10234 t
ORIGIN      
        1 ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag tgctagaata
       61 atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa gtccccaaat
      121 agattcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg aatatcaatt
      181 tcatctataa ggaaggtagc taattgaact aacttattta ttctgtttgt cgctagaaaa
      241 atcagacctc cttgtgaaga ttgaggagat acttaatgaa aatcaaagaa gaaactagca
      301 agctagtagc agattgccca aaacaccgct ttgaggttgt agataagact gacctatata
      361 atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtataggc tagagagtag
      421 tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact tggataggaa
      481 aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc cgtttaggaa
      541 cttatgtggg agtaaatcca cattttgcaa cattaataga ttttctagaa aaaacaggac
      601 tagaaaattt aacagaaggt tcgattgcta tcgatggtga tcgattgttt gggaattgct
      661 ttacttatct agcagatggt caagcaggag ccttctttga aacccaccaa aaatatttgg
      721 atattcattt agtgctggaa aacgaagaag ccatggctgt gacgtcgccg gaaaatgtaa
      781 gcgttaccca agagtatgat gaagagaagg atattgaatt atacacaggg gaagtggaac
      841 agttagttca tttgagagct ggcgaatgcc tcatcacttt tccagaagat ttacatcaac
      901 ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt gcgatttctt
      961 aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta gctggaattg
     1021 cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca actagtactg
     1081 aaccaccaac agaattatct ggtgagatta caatgtggca ctcctttact caaggacccc
     1141 gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca aaaacgaaaa
     1201 tcaagattga aacattttct tggaatgact tctatactaa atggactaca ggtttagcaa
     1261 atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa atggtcaact
     1321 cagatgcttt ggttccgcta aatgattcta tcaagcgtat tggacaagat aaatttaacg
     1381 aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct ctttattcac
     1441 atgcacaagt catgtgggtt agaacagatt tgttaaaaga acataatatt gaggttccta
     1501 aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga gtttatggct
     1561 tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac ttctacgtac
     1621 gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca agccaacttg
     1681 ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct caagattctt
     1741 tgaactttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca gcatttgact
     1801 ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg attgattcga
     1861 ttgatgctta tcctattcca aaaatcaaag agtctgataa agaccaagga attgaaacct
     1921 caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct aaagcattct
     1981 tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact ccagtaggta
     2041 tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat gaaactcgta
     2101 agaaatttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt actgctattg
     2161 gttatgaaaa tggaccaagt gtacaagctg gtatgttaac taaccaacac attattgaac
     2221 aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca aaagaagcag
     2281 aaaaacaatt aaacgattta tttgaggctg ttcagtagat gtaaaagact agaaaatagg
     2341 tgggctagtg agctgaaaag ctctagccca atcttgtaaa agaagggaga aggagaatgg
     2401 ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt atcgtaggat
     2461 tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat aagcatttga
     2521 ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta tcagatccca
     2581 acttctttaa tgcgttcttt aattcaatta agtggaccgt tttctcatta gttggtcaag
     2641 ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc aagaaattat
     2701 ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc ttctcttggc
     2761 agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa ttaggtttaa
     2821 tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt ttggtgttta
     2881 tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct ttgcaaacag
     2941 taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg caggtgttca
     3001 agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt ttgagaactg
     3061 tatggatctt taataacttt gacattatct acctcattac tggtggtgga ccagccaatg
     3121 ctacaacgac gcttccaatt tttgcttaca acctgggctg gggaactaaa ttgttgggtc
     3181 gtgcttcagc agttacagta ttgctcttta tcttcttggt ggcgatttgc tttatctact
     3241 ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga aatccggtat
     3301 ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg cagttttccc
     3361 attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc agtatccaac
     3421 acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca acgatttgca
     3481 cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc ttattgcgat
     3541 tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat tgggagcaat
     3601 catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag caattcccta
     3661 ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga tgatggttta
     3721 tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc aaacagttcc
     3781 aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta cgttttataa
     3841 agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata catttatcaa
     3901 tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa agatgacagt
     3961 agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag atatgatggc
     4021 agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc aaaataagat
     4081 tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa aatgaataaa
     4141 agaggtcttt attcaaaact aggaatttct gttgtaggca ttagtctttt aatgggagtc
     4201 cccactttga ttcatgcgaa tgaattaaac tatggtcaac tgttcatatc tcctattttt
     4261 caaggaggtt catatcaact gaacaataag agtatagata tcagcccttt gttattagat
     4321 aaattgtctg gagagagtca gacagtagta atgaaattta aagcagataa accaaactct
     4381 cttcaagctt tgtttggcct atctaatagt aaagcaggct ttaaaaataa ttacttttca
     4441 attttcatga gagattctgg tgagataggt gtagaaataa gagacgccca aaagggaata
     4501 aattatttat tttctagacc agcttcatta tggggaaagc ataaaggaca ggcagttgaa
     4561 aatacactag tatttgtatc tgattctaaa gataaaacat acacaatgta tgttaatgga
     4621 atagaagtgt tctctgaaac agttgataca tttttgccaa tttcaaatat aaatggtata
     4681 gataaggcaa cactaggagc tgttaatcgt gaaggtaagg aacattacct cgcaaaagga
     4741 agtattgatg aaatcagtct atttaacaaa gcaattagtg atcaggaagt ttcaaatatt
     4801 cccttgtcaa atccctttca gttaattttc caatcaggag attctactca agctaactat
     4861 tttagaatac cgacactata tacattaagt agtggaagag ttctatcaag tattgatgca
     4921 cgttatggtg ggactcatga ttctaaaagt aagattaata ttgccacttc ttatagtgat
     4981 gataatggga aaacgtggag tgagccaatt tttgctatga agtttaatga ctatgaggag
     5041 cagttagttt actggccacg agataataaa ttaaagaata gtcaaattag tggaagtgct
     5101 tcattcatag attcatccat tgttgaagat aaaaaatctg ggaaaacgat attactagct
     5161 gatgttatgc ctgcgggtat tggaaataat aatgcaaata aagccgactc aggttttaaa
     5221 gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga tttccgttat
     5281 acagttagag aaaatggtgt cgtttatgat gaaacaacta ataaacctac aaattatact
     5341 ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga acaatattcg
     5401 gttgattttg atagtggctc tttaagagaa aggcataatg gaaaacaggt tcctatgaat
     5461 gttttctaca aagattcgtt atttaaagtg actcctacta attatatagc aatgacaact
     5521 agtcagaata gaggagagag ttgggaacaa tttaagttgt tgcctccgtt cttaggagaa
     5581 aaacataatg gaacttactt gtgtcctgga caaggtttag cattaaaatc aagtaacaga
     5641 ttgatttttg caacatatac tagtggagaa ctaacctatc tcatttctga tgatagtggt
     5701 caaacatgga agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc agaagcacaa
     5761 atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac aggtaagata
     5821 gcttatatga ctagtagaga ttctggagaa acatggtcgg aagtttcgta tattgatgga
     5881 atccaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca attaattgat
     5941 ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa gggaggtcaa
     6001 ttagttgtcg gtttagtcaa taaagaagat gatagtattg attggaaata ccactatgat
     6061 attgatttgc cttcgtatgg ttatgcctat tctgcgatta cagaattgcc aaatcatcac
     6121 ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa atgaattgca tttaagcaat
     6181 gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag aaaaacatgg
     6241 ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc tatatgcaaa
     6301 agaatgatgg agcagagatt actcttctct atgatccaga taatgcagag gcgattgcag
     6361 aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc gatgaagtag
     6421 attgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt aaggctgcac
     6481 agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa gattgtcgcg
     6541 agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat attatgaatt
     6601 tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc ggagacgttc
     6661 tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta tcatggaaaa
     6721 aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg gattgcgttc
     6781 aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat gtggcccatg
     6841 aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa ttttctaata
     6901 agcgttttgc cttgttagaa tggggttcag cttatcgttg gggtgaacat tatgtcttaa
     6961 tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga actcttaagc
     7021 tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat gatgatcgga
     7081 ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa ccaggtaaac
     7141 gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg catgagatta
     7201 tggaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa gctgccctag
     7261 aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc aaagtaaaat
     7321 tgtcagaaat tgtaaaataa attttggtat tctcctatta taggtcgact tgctcctctg
     7381 aaagtacttt tagaggagct gtttgacttt gctagttttt gaaactgaaa tctattatac
     7441 tacaaactat tgaaagcgtt ttaattttaa ggtataataa tctcatagaa ataaagaaaa
     7501 ggaggaaaga ggatgccaca gattagcaaa gaagccttga ttgagcaaat caaagatgga
     7561 atcatcgttt cttgtcaggc tcttcctcat gaaccgcttt atacagaagc gggaggggtg
     7621 attcccttgc tggtcaaagc ggctgagcaa ggtggagcag tcggtatccg agcaaacagt
     7681 gttcgcgata tcaaggaaat taaggaagtc actaaacttc caatcattgg gattatcaaa
     7741 cgtgattatc cacctcagga acccttcatc acggctacta tgaaagaagt tgatgaattg
     7801 gcagaactgg acatcgaggt gattgctctg gattgtacca agcgtgaacg ctacgatggt
     7861 ttggaaattc aagagttcat tcgtcaggtt aaggagaaat atcctaatca gcttttgatg
     7921 gctgatacta gtatcttcga agaagggcta gcagctgtag aagcaggaat tgactttgtc
     7981 ggaacaacct tatcaggcta cacatcctac agtccaaaag tagacggtcc agattttgaa
     8041 ttgattaaga aactctgtga tgctggtgta gatgtcattg cagaaggaaa aattcataca
     8101 ccagaacaag ccaaacaaat ccttgaatat ggagtgcgag gcatcgttgt tggtggcgcc
     8161 attactagac caaaagagat tacagaacgc ttcgttgcta gtcttaaata agatgtgagg
     8221 gggagtttta tgtttaaagt tttacaaaaa gttggaaaag cttttatgtt acctatagct
     8281 atacttcctg cagcaggtct acttttgggg attggtggtg cactttcaaa cccaaccacg
     8341 atagcaactt atccaatact agacaatagt atttttcaat cgatattcca agtaatgagc
     8401 tctgcaggag aggttgtatt cagtaatttg tcactacttc tctgtgtggg attatgtatt
     8461 ggcttagcga aacgagataa aggaaccgct gcgttagcag gagtaactgg ttacttagtt
     8521 atgactgcaa cgatcaaagc tttggtaaaa ctttttatgg cagaaggatc tgcaattgat
     8581 actggagtta ttggagcatt agctgtcgga atagttgccg tatatttgca caaccgatat
     8641 aacaatattc aattaccttc cgctttagga ttctttggag gttcacgctt cgttcctatt
     8701 gttacatcgt tctcttctat cttgattggc tttgtcttct ttgttatttg gccacctttc
     8761 caacaacttc ttgtttctac aggtggatat atttctcagg cgggtccaat tggaactttt
     8821 ctatatggat ttttaatgag actttctgga gcagtaggct tacatcatat aatttaccct
     8881 atgttttggt atactgaact tggtggtgtt gaaactattg caggacaaac agtggttgga
     8941 gctcaaaaaa tattttttgc tcaattagcc gatccggccc attctggatt atttacagaa
     9001 ggaacaaggt tttttgcagg tcgtttctca acaatgatgt tcggtttacc ggctgcctgt
     9061 ttagcgatgt accatagtgt tcctaaaaat cgtcgtaaaa aatacgcggg tttgtttttt
     9121 ggagttgctt taacatcttt tattaccggt attacagaac caattgaatt tatgtttcta
     9181 ttcgtcagtc cggttctata tgttgttcac gcattccttg atggtgttag cttctttatt
     9241 gcagacgtct taaatatttc aataggaaac acattttcag gaggtgtaat cgatttcact
     9301 ttatttggaa ttttgcaggg gaacgctaag acgaattggg ttcttcagat tccaattgga
     9361 cttatttgga gtgttttgta ttatattatt tttagatggt tcattactca attcaacgtt
     9421 ctaacgccag gacgaggaga agaagtagat tctaaagaaa tttctgaatc cgcagattca
     9481 acttcaaata ctgcagatta tttaaaacag gatagcctac aaattatcag agccttgggt
     9541 ggatcaaata atatagaaga tgtagatgct tgtgtgacac gtttacgtgt agctgtaaaa
     9601 gaagttaatc aagttgataa agcactttta aaacaaattg gtgcagttga tgtcttagaa
     9661 gtgaagggtg gcattcaagc aatctatgga gcaaaagcaa tcttatataa aaatagtatt
     9721 aatgaaattt taggtgtaga tgattaagta cttactgact taataaaaaa cagaggagag
     9781 tgatggatga gtaggatgaa atgaaatcgc atacaagaaa taaagaactc attatccaag
     9841 ttggatacgc ttattacata ggagaataca aatgaaattt agaaaattag cttgtacagt
     9901 acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc aattctggcg gaagtaaaga
     9961 tgctgccaaa tcaggtggtg acggtgccaa aacagaaatc acttggtggg cattcccagt
    10021 atttacccaa gaaaaaactg gtgacggtgt tggaacttat gaaaaatcaa tcatcgaagc
    10081 gtttgaaaaa gcaaacccag atataaaagt gaaattggaa accatcgact tcaagtcagg
    10141 tcctgaaaaa atcacaacag ccatcgaagc aggaacagct ccagacgtac tctttgatgc
    10201 accaggacgt atcatccaat acggtaaaaa cggtaaattg gctgagttga atgacctctt
    10261 cacagatgaa tttgttaaag atgtcaacaa tgaaaacatc gtacaagcaa gtaaagctgg
    10321 agacaaggct tatatgtatc cgattagttc tgccccattc tacatggcaa tgaacaagaa
    10381 aatgttagaa gatgctggag tagcaaacct tgtaaaagaa ggttggacaa ctgatgattt
    10441 tgaaaaagta ttgaaagcac ttaaagacaa gggttacaca ccaggttcat tgttcagttc
    10501 tggtcaaggg ggagaccaag gaacacgtgc ctttatctct aacctttata gcggttctgt
    10561 aacagatgaa aaagttagca aatatacaac tgatgatcct aaattcgtca aaggtcttga
    10621 aaaagcaact agctggatta aagacaattt gatcaataat ggttcacaat ttgacggtgg
    10681 ggcagatatc caaaactttg ccaacggtca aacatcttac acaatccttt gggcaccagc
    10741 tcaaaatggt atccaagcta aacttttaga agcaagtaag gtagaagtgg tagaagtacc
    10801 attcccatca gacgaaggta agccagctct tgaatatctt gtaaacgggt ttgcagtatt
    10861 caacaataaa gacgacaaga aagtcgctgc atctaagaaa ttcatccagt ttatcgcaga
    10921 tgacaaggag tggggaccta aagacgtagt tcgtacaggt gctttcccag tccgtacttc
    10981 atttggaaaa ctttatgaag acaaacgcat ggaaacaatc agtggctgga ctcaatacta
    11041 ctcaccatac tacaacacta ttgatggatt tgctgaaatg agaacacttt ggttcccaat
    11101 gttgcaatct gtatcaaatg gtgacgaaaa accagcggat gctttgaaag ccttcactga
    11161 aaaagcaaac gaaacaatca aaaaagctat gaaacaatag tccttagtta ttctatgaaa
    11221 aagtaatttt ttaaagaacc taagagtgta tacccccttt tccctctaca cagatagtgt
    11281 aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa atgaagttct
    11341 tacataagcg aaccataaaa aatttcattt tgattttaaa acagttcaag aaagtcaaaa
    11401 aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc gtatgcggga
    11461 aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca tctttgtgtt
    11521 ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga ctaaatttga
    11581 gtttgtaggc ttggataact atatccgtat gtttaaagat cctgtcttta caaaatctct
    11641 gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat tctcactctt
    11701 tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc gtttcgtctt
    11761 cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat ggatttatga
    11821 cccactatca gggattctaa actttgtcct taagtcaagc cacatcatca gccaaaacat
    11881 ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc tcttgaccac
    11941 ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg acaattcact
    12001 ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga agattaaatg
    12061 gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta actcattcca
    12121 gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa caagtacctt
    12181 gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg ccaacacaat
    12241 tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta aagtacttgg
    12301 aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa aaaaaccatt
    12361 aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc tgttcatctt
    12421 tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa ttgttattcc
    12481 tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca tggtgcagaa
    12541 ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca tgttcttagt
    12601 ttgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct atggtcaacg
    12661 cattctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg tccttgtacc
    12721 attggtacgt atcgtcaact tcatgggaat ccacgatact ctctgggcag ttatcttgcc
    12781 tttgattgga tggccattcg gtgtcttcct catgaaacag ttcagtgaaa atatccctac
    12841 agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct tctggagtgt
    12901 agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct tcatcaatac
    12961 ttggaatgac tacttcatgc agttggtaat gttgacttca cgtaacaatt tgaccatctc
    13021 acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga ttatggcagg
    13081 agctgccctt gctgctgttc caatcgtcac agtcttccta gtcttccaaa aatccttcac
    13141 acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatcg aatgcaaact
    13201 acgtcagctt caccttgcca tacttaagta ttgcctgtgg ttagcttcct agtttgttct
    13261 tcaattttca ttgagtatag gaaaatcaat ctatcaaaat acagaagtat attttataga
    13321 tttagagaat atagaagtta taagtgtcta caaaatggag ggtatgcagt tactttatga
    13381 agttttgtca gacacttatc aacttaagaa tggttttagt taactatcag aaacgaagga
    13441 aagagtatga tttttgacga tttgaaaaac atcacctttt acaaagggat tcatcccaat
    13501 ttagacaagg ctatcgacta tctctaccaa catcgtaaag attcattcga attaggaaag
    13561 tatgagattg atggagataa agtctttcta gttgttcagg aaaatgtcct caatcaagtt
    13621 gagaataatc aatttgaaca ccataagaac tatgcagatt tgcatttgct ggtagaagga
    13681 catgaatatt cgagctacgg ttcacgtatc aaagacgagg cagtagcatt cgacgaagcg
    13741 agtgacattg gctttgttca ttgtcatgaa cactacccac tcttgttggg ttatcacaat
    13801 tttgcgattt tcttcccagg tgagccacat cagccaaatg gttatgcagg catggaagaa
    13861 aaggttcgaa aatatctctt taaaattttg attgattaaa aataggatga attgtttttt
    13921 tgtaaagctt tgacaatact ctaccatgaa attgatcttt gtgaggtagg gaaatgagaa
    13981 taaaatattt aaaaattggt atcttcttgt cagtctgtgt atgctacaag agctagtttc
    14041 ttggatggac agggaattac agttgatgag atggcttgga taattagggg cattgtgaat
    14101 gcattgattg gtagatacat aaaattaggt acttatgcgg ctaagtatgg tattagtatg
    14161 gcgcgttcta tcttaaatag ggcagctgca actgcggcag caagagtagg attattgact
    14221 aagatttttg gatggatttt acgaatagct gtgctcgcag ctgatgtata tggtaatttt
    14281 gccaacaata ttgctgtagc ttgggatgca catgataaaa tccctaacaa tggtcgtata
    14341 aacttttaaa atgcgagaat gaaagcactt tgtatttttt tattgaatat gttagcttgg
    14401 acagtgcttg caatgataat ccgtggaggg ctagatggat ttgataggca tacttggagt
    14461 actattttaa ttgcttcgct gttcggggta tatgattata agcccataga taaaaataga
    14521 aaaaagtcca aaagaaaaaa gaaaaaatag atttgttcat ggtagggact tatgaaagct
    14581 ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat ggaggagcaa acatggcaca
    14641 aaaaggagta agccttatca aggcagcatt tgatacagat aactttctca tgcgttttag
    14701 tgagaaagtc ttggacatcg tgacagccaa tcttcttttt gtcgtctctt gtttgcccat
    14761 cgtgacgatt ggagtggcta aaatcagcct ctacgagacc atgttcgaag ttaagaagag
    14821 cagacgggta cctgttttta aaatctatct aagatctttc aagcaaaatc tgaaactagg
    14881 tcttcagctg ggtttaatgg agttaggaat tgtgtttctt accctttcag atctctatct
    14941 tttctggggt caaacagctc tgcccttcca attgctgaaa gccatttgtt taggtattct
    15001 gatttttctt actatcgtga tgctggctag ttaccctatc gcggcacgtt atgacctatc
    15061 ttggaaagaa gtgttacaaa aagggcttat cttggcaagt tttaactttc cttggttctt
    15121 cctcatgtta gccattcttg tcctcattgt gatggttctt tatctgtctg ccttcagtct
    15181 actcttaggt ggctcagtct tcctgctttt tgggtttgga ctattggtct ttatccagac
    15241 tggattgatg gagaaaattt tcgcaaaata ccaataggag ccttatttct gaaactactt
    15301 tcaaaggctc caaacgctat tctataagcg agaaactaaa atctaagtgt ctataagata
    15361 ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa gaaaaatctg ctttagcaga
    15421 aaataaaaat aataggagaa aatctatgtc agatttgaaa aaatacgaag gtgtcattcc
    15481 agccttctac gcatgttatg atgagcaagg agaagtaagt ccagagcgta cgcgtgcctt
    15541 ggttcaatac ttcattgata aaggtgttca aggtctttat gtcaatggtt cttctggtga
    15601 atgtatctac caaagcgttg aagatcgcaa gttgattttg gaagaagtca tggcggtagc
    15661 caaaggtaaa ttgaccatta ttgcccatgt tgcttgcaat aatactaaag atagtatgga
    15721 acttgctcgc catgctgaaa gcttgggagt agatgctatt gcaacgattc caccaattta
    15781 cttccgcttg ccagaatact cagttgccaa atactggaac gatattagtt ctgcagctcc
    15841 aaacacagac tacgtgattt acaacattcc tcaattggca ggggttgctt tgactccaag
    15901 cctttacaca gaaatgttga aaaatcctcg tgttatcggt gttaagaact cttcaatgcc
    15961 agttcaagat atccaaacct ttgtcagcct tggcggggaa gaccatattg tctttaacgg
    16021 tccagatgaa cagttcctag gtggccgcct catgggtgct aaggctggta tcggtggtac
    16081 ttatggtgct atgccagaac tcttcttgaa actcaatcag ttgattgcgg ataaagattt
    16141 agaaacagcg cgtgaattgc agtatgctat caacgcaatc attggtaaac tcactgctgc
    16201 acatggaaat atgtacggtg tcatcaaaga agttttgaag atcaatgaag gcttgaatat
    16261 tggttctgtt cgttcaccat tgacaccagt gactgaagac gatcgtccag ttgtagaagc
    16321 ggctgcagcc ttgattcgtg aaaccaagga gcgcttcctc taatctaaaa ggaggtattt
    16381 atgacatact acgttgcaat tgatatcggt ggaaccaaca tcaagtatgg tttggttgat
    16441 caagaggggc aacttcttga atcgcatgaa atgccaactg aggcgcataa gggtggacct
    16501 catatcttac aaaagaccaa agatatcgta gctagttatt tagaaaaagg cccagtagca
    16561 ggtgttgcca tatcttctgc tgggatggtg gatccggata agggtgagat tttctatgct
    16621 gggcctcaaa tccctaacta cgcaggcacc cagttcaaaa aggaaatcga agaaagcttt
    16681 actattcctt gtgagattga aaatgatgtc aactgtgcag gtcttgctga ggcagtatct
    16741 ggttcaggca agggagcaag tgtgacactt tgcttgacca ttggaaccgg tatcggtggt
    16801 tgcttgatta tggataggaa agtcttccat ggttttagca attcagcctg tgaagtcggg
    16861 tatatgcata tgcaggatgg agcttttcag gatctcgctt ctacaacagc tttagtgaaa
    16921 tatgtagctg aagcccatgg agaagatgtt gatcagtgga atggccgacg tatctttaag
    16981 gaagctactg aaggaaacaa aatctgcatg gaaggtattg accgtatggt tgactatcta
    17041 ggaaaaggtc tggcaaatat ttgttatgtg gccaatccag aagtggttat tcttggtggt
    17101 ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc gcacagcctt gaaagaggca
    17161 ttggtaccaa gtttagcaga aaaaacacga ttagaatttg cccatcacca aaatacagca
    17221 gggatgttgg gtgcatatta tcattttaag acaaaacaat cctagtttgg ctcagccaaa
    17281 ctaggatttt cttacacgtt tttgtctacg atagccgttg agttttttat tttcccagta
    17341 gctattaaag attttttcct tgctttcgcg attgatttcc aaaaagtagg cataaatcaa
    17401 atcgataaag aagagcatag gaagttgagc ggatattcgt tggatgtagg agggttggct
    17461 gtgggtggct acaagaacag tctctgtata ggtctggcta tctttattgg gaacacttgt
    17521 aaagagtaca gtctttgccc ccatctcctt ggcatctaat agactatcta aaatagaagg
    17581 agttgagcct gaaagtgaga aacccagtac tagacaattt tcatccatga tgctggttgt
    17641 ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc acacctagtc gcataaaacg
    17701 taatttcatt tcacgggcga cgaggccaga actccctgtt ccaaagaagt agatacgctc
    17761 agcatcttcg attagctggg caattcgctc tagttggatt tcgtcaatca agtcttgtgt
    17821 ttgttccctc atattgctat aacttctgag gactcgtttg gtcagtggac tatgcttgga
    17881 gacttggttg gcttgatttt ctgcctgatg ttggtattgg aaaataaatt ctcggtagcc
    17941 agtaaagcca cactttttag caaagcgggt caaagcagct tgagaaatat gtaatttttg
    18001 ggtgacttgt tgagaagata aatcatctgt aatcgtttca gcttgcaaaa aatagcgagc
    18061 gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa tcaatgatag ttgcgatatc
    18121 tggtttgtcc atagggaaag ctcctttaca tgggtcatac tggaagacta gatcagggaa
    18181 tagtcacact tcattataac acataatata aggatagata aataaaaacg catctctgtt
    18241 ttaaaaacga aaaaatcgaa aaagcttctc tcttttccat aattttctac tcaaattgtg
    18301 gtacaattaa gagtaagatt ttaagttaga aatgagactg atttgtatga gaaaatttaa
    18361 cagccattcg attccgattc ggcttaattt attgttttca atcgtcattt tactctttat
    18421 gaccattatt ggtcgtttgt tgtatatgca ggttttgaac aaggattttt acgaaaaaaa
    18481 gctagcctca gctagtcaga ccaagattac aagcagttca gcccgtgggg aaatttatga
    18541 tgctagtgga aaacctttgg tagaaaatac gttaaagcag gttgtttcct ttacgcgtag
    18601 caataaaatg acggctacag acttaaaaga aacagctaaa aagttactga cttatgtgag
    18661 catcagttct ccaaatttga cagaacgcca gctggcggat tactatttgg ctgatcctga
    18721 aatctataaa aaaacagtgg aagctctccc aagtgagaaa cgcttggatt cagatggcaa
    18781 tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat agtgtccaaa caagtcaact
    18841 aaactataca gaggatgaaa agaaagaaat ctatcttttt agtcagttaa atgctgttgg
    18901 aaactttgcg acaggaacca ttgctacaga tcctctaaat gattctcagg tggctgttat
    18961 tgcctctatt tcaaaggaga tgcctggcat tagtatttct acttcttggg atagaaaggt
    19021 tttggaaact tccctttctt ctatagttgg gagtgtatcc agtgaaaaag ctggtctccc
    19081 agcggaagaa gcagaagcct atcttaaaaa aggctattct ctaaatgatc gtgtaggaac
    19141 ctcctatttg gaaaagcaat atgaagagac cttacaagga aaacgctcgg taaaagaaat
    19201 ccatctggat aaatatggca atatggaaag cgtggataca attgaggaag gtagtaaggg
    19261 aaacaatatc aaactgacca ttgatttggc tttccaagat agcgtggatg ctttactgaa
    19321 aagttatttc aattccgagc tagaaaatgg tggagccaag tattctgaag gtgtctatgc
    19381 agtcgccctt aacccaaaaa caggtgcggt tttgtctatg tcagggatta aacatgactt
    19441 gaaaacggga gagttgacgc ctgattcctt gggaacggta accaatgtct ttgttccagg
    19501 ttcggttgtc aaggcggcga ccatcagctc aggttgggaa aatggagtct tgtcaggaaa
    19561 ccagaccttg acagaccagt ccattgtctt ccaaggttca gctcccatca attcttggta
    19621 tactcaggct tacggttcat tccctatcac agcggtccaa gctctggagt attcatcaaa
    19681 tacctatatg gtccaaacag ccttaggtct tatggggcaa acctatcaac ccaatatgtt
    19741 tgtcggcacc agcaatctag agtctgctat ggagaaactg cgttcaacct ttggcgaata
    19801 tggcttgggt actgcgacag gaattgacct accagatgaa tctactggat ttgttcccaa
    19861 agagtatagc tttgctaatt acattactaa tgcctttggg cagtttgata actatacgcc
    19921 gatgcagttg gctcagtatg tagcaactat tgcaaataat ggtgttcgtg tggctcctcg
    19981 tattgttgaa ggcatttatg gtaataatga taagggagga ctgggtgact tgattcagca
    20041 actgcaaccg acagagatga ataaggtcaa tatatccgac tccgatatga gcatcttgca
    20101 ccaaggtttt tatcaggttg cccatggtac tagtggattg acaactggac gtgccttttc
    20161 aaatggcgcc ttggtatcca ttagcggaaa aacaggtaca gccgaaagct atgtggcaga
    20221 tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc ccatctgata atccccaaat
    20281 cgctgttgca gtggtctttc ctcataatac caatctaaca aatggtgtag gaccttccat
    20341 tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca atgaactaga aaggaaatta
    20401 tgctttatcc aacacctatt gccaagttga ttgacagtta ttctaagtta ccaggtatcg
    20461 ggattaagac ggctacgcgt ctggcctttt atacgattgg gatgtctgct gatgatgtca
    20521 atgaatttgc aaaaaatctc ctttctgcta agagagaatt gacatattgt tctatttgtg
    20581 gacgtttgac agacgacgat ccttgttcta tctgtactga tccgactcgt gaccagacaa
    20641 caattttagt tcttgaggat agtagagatg tggcagccat ggaaaatatc caagaatacc
    20701 atggactcta tcatgtcctt catggcctca tttctcctat gaatggtatc agtccggacg
    20761 atatcaatct caagagcctt atgactcgtc ttatggatag tgaagtttca gaagtgattg
    20821 tggcgactaa tgctacagcg gatggtgaag cgacttccat gtatctttca cgtttgctca
    20881 agccggctgg tatcaaggtt acgcgtctag cacgaggtct cgctgtggga gcggacattg
    20941 agtatgcgga cgaagtgaca ctcttacgag cgattgaaaa tcggacagag ttgtaagtgt
    21001 aggcaaattt acgaactcca ttcatttata aaaaatcaaa gaggctgaaa atcgttccta
    21061 tcggcctctt tttgtatagt gtgatgagta ggatcaggtt caagttttaa aaaaccaagc
    21121 aaatatgata tactaaagag cgagtattct agtagaatta ggacaaataa tatgaaacaa
    21181 acgattattc ttttatatgg tggacggagt gcggaacgcg aagtctctgt cctttcagct
    21241 gagagtgtca tgcgtgcggt caattacgac cgtttcacag tcaagacttt ctttatcagt
    21301 cagtcaggtg actttatcaa aacacaggaa tttagtcatg ctccggggca agaagaccgt
    21361 ctcatgacca atgaaaccat tgattgggat aagaaagttg caccaagtgc tatctacgaa
    21421 gaaggtgcag tggtctttcc agtccttcac gggccaatgg gagaagatgg ctctgttcaa
    21481 ggattcttgg aagttttgaa aatgccttac gttggttgca acattttgtc atcaagtctt
    21541 gccatggata aaatcacgac taagcgtgtt ctggaatctg ctggtattgc ccaagttcct
    21601 tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa tcgctgaagt ggaagaaaaa
    21661 ttggcttatc cagtcttcac taagccgtca aacatggggt ctagtgtcgg tatttctaag
    21721 tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg ccttccgata tgacagccgt
    21781 gtcttggttg agcaaggagt gaatgcccgt gaaattgagg ttggcctctt gggtaactac
    21841 gatgtcaaga gcacgctacc tggagaagtt gtcaaggacg ttgcctttta tgactacgat
    21901 gccaagtata ttgataacaa gattactatg gatattcctg ccaaaatcag tgatgatgtg
    21961 gtggctgtca tgcgtcaaaa tgcagaaaca gccttccgtg ccattggtgg ccttggtcta
    22021 tctcgttgcg atttcttcta tacagataag ggagagattt ttctcaacga gctcaatacc
    22081 atgccaggtt ttacccagtg gtctatgtac ccactacttt gggacaatat ggggatcagc
    22141 tccccagaac taatcgagcg tttggttgac cttgccaagg aaagttttga caagcgcgaa
    22201 gcgcatttga tataaaatga aagagagggt agaagccaga accatcactg cacggtgact
    22261 agagttctcg gacttcagcc ctttttaaag gagtagaaat gaaattaaca atccatgaaa
    22321 ttgcccaagt tgttggagcc aaaaatgata tcagtatctt tgaggacacc cagttagaaa
    22381 aagctgagtt tgatagtcgt ttgattggaa ctggagattt atttgtgcca cttaaaggtg
    22441 cgcgtgatgg ccatgacttt attgaaacag cctttgaaaa tggtgcagca gtaaccttgt
    22501 ctgagaaaga ggtctcaaat catccttaca ttctagtaga tgatgttttg acagcctttc
    22561 aatccctagc atcctactat cttgaaaaaa cgactgttga tgtctttgct gttacaggtt
    22621 caaatggcaa gacaacgact aaggatatgt tggcgcattt actgtcaaca agatacaaga
    22681 cctacaaaac acaaggcaat tacaataatg agattggcct tccttacaca gttctccaca
    22741 tgcctgaagg aacagaaaag ttggtcttgg agatggggca ggaccacttg ggagatattc
    22801 atctcttgtc tgaattggct cgtccaaaaa cagccatcgt gaccttggtt ggagaagctc
    22861 atttggcctt tttcaaagat cgttctgaga ttgctaaagg aaaaatgcaa attgcagacg
    22921 gaatggcttc aggttccttg cttttagcgc cggctgaccc tatcgtagag gactatttgc
    22981 caactgataa aaaggtggtt cgttttggtc aaggagcaga gctggaaatt actgacttgg
    23041 ttgagcgcaa agatagtctg accttcaagg ccaatttctt agagcaagtc cttgatttgc
    23101 cagtaactgg caagtacaat gcgacaaatg ctatgattgc atcctatgtt gccttgcaag
    23161 aaggagtttc agaggagcaa attcatcagg ccttccaagg tcttgaattg acgcgtaatc
    23221 gtactgagtg gaagaaagca gccaatggag cagatatcct gtcagacgta tataatgcca
    23281 atccaactgc tatgaagctg attttagaga ctttctctgc cattccagcc aatgaaggtg
    23341 gcaagaaaat tgcagtgttg gcggatatga aggagcttgg tgaccagtct attcaactcc
    23401 ataaccagat gattttgagc ctttctccag atgtgcttga taccgttatt ttctatggag
    23461 aaaatattgc tgaattagcc caattggcta gtcaaatgtt cccaataggc cacgtttact
    23521 acttcaagaa aacagaagac caagaccaat ttgaagacct agtcaagcag gtcaaagaaa
    23581 gccttggagt ccacgaccaa atcctgctca aaggctctaa ctctatgaat ctagccaagt
    23641 tggtagaaag tttagaaaat gaaaccaagt gattttgtta agtatctgca aagaatgatt
    23701 gcccttacag atactggctt aacctttaca aaagatcctt ttgaccgtga gcgctacgaa
    23761 gacttgcgaa gtctgttatc tgaaatgttg aatcaggtat cagacctgga tgcagaagaa
    23821 gtagcagaag tcttgaaacc aacttcagct tatgcgactc ccttaatgga cgtccgtgct
    23881 tggattgttg aggatgagaa gatttgtctg gttaggggac aaggagagga tagttgggct
    23941 ttgccaggtg gctttggtga agtcggctat tctccaactg aaaatattct caaggaaatt
    24001 gaagaagaaa ccggttttaa agccaaagtt gaaagactgc tagctgtttt tgataccaat
    24061 cgtttccaac tacagagcaa acaatatgca aagtttgttt ttgaatgtaa gcttctcgat
    24121 ggacaattcc aagaaaatca agaaatcgct gcccttcaat tttttgcaat tgaccaactg
    24181 ccgaatttat ctgaaaaacg cattaccaag gagcaaatcg agattctttg gcaggtttat
    24241 caaggtcaga gggagcaata tcttgactaa gaagatgatt atcgtatttc taaatccatt
    24301 tttgacaact agcatggtat aataatatgc aggaaaattt tgaatcatga ggaagactag
    24361 atgaatttat gggatatttt ctttacgact caggcaaccg agccgcccaa atttgacctt
    24421 ttttggtatg ttagcctatt tacgctctta gccttaacct tttatacagc ccatcgctat
    24481 cgtgaaaaga aggtttacca acgatttttc caaatcttgc agactgttca gttaatcctt
    24541 ctttatggtt ggtactgggt caatcatatg ccactgtcag aaagcctacc cttttaccat
    24601 tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc agtccaaata taaacaatac
    24661 tttgcattat tgggaacatt tgggacatta gcagcctttg tttatccagt gccagatgct
    24721 tacccttttc cacatatcac cattctatcc tttatctttg gtcatttagc actcttgggg
    24781 aactctctag tttatctatt gagacagtat aatgcgcgat tgctggatgt gaagggaatt
    24841 tttctcatga cctttgccct aaatgccttg atttttgtgg tcaatttggt gacaggtggc
    24901 gattacggat ttttgacaaa accgccattg gttggggatc acggtctagt agctaattat
    24961 ttacttgttt caattgtgct ggtagctact atcagtttga ctaagaaaat cttagaattc
    25021 tttttagctc aagaagcaga aaaaatgatt gcaaaggaag cttaacacag agctttcttt
    25081 tttgctctta gagagttttt acaagcagct tataaaataa gaatttctga atagacaaac
    25141 tcaaaaaatg gctgggaaat ttaggaaaaa agcaagcacg attaaatttt ttgtgttata
    25201 atattttgtg aatagctatg cctatgttta gctatggaat aatacgaagt gcgaaacttg
    25261 gaagatagag aggaagcgat gtaatggcta gagaaggctt ttttacaggt ctagatattg
    25321 gaacaagctc tgtcaaggtg cttgtggccg agcagagaaa tggtgaatta aatgtaattg
    25381 gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat tattgttgat attgatgcag
    25441 cagcaactgc tatcaagtca gccatttccc aagcggaaga aaagtcaggc atttcgatta
    25501 aatcagtaaa tgtcggcttg cctggtaatc ttttgcaggt agaaccaact caggggatga
    25561 ttccagtaac atctgatact aaggaaatta cggatcaaga tgttgaaaat gttgtcaaat
    25621 cagctttgac aaagagtatg acacctgacc gtgaagtcat tacctttatt cctgaagaat
    25681 ttattgtgga tggtttccaa gggattcgtg acccacgtgg catgatgggg gttcgccttg
    25741 aaatgcgtgg tttgctttat acaggacctc gtactatctt gcacaatttg cgtaagacgg
    25801 ttgagcgtgc aggtgttcag gttgaaaatg ttatcatttc accactagca atggttcagt
    25861 ctgttttgaa cgaaggggaa cgtgaatttg gtgctacagt gattgatatg ggggcaggtc
    25921 aaacgactgt cgctacaatc cgtaatcaag aactccagtt cacacatatt ctccaagaag
    25981 gtggagatta tgtaactaaa gatatctcca aggttttgaa aacctctcgc aaattagcgg
    26041 aaggcttgaa actgaattac ggggaagcct atccgcctct tgcaagcaaa gaaaccttcc
    26101 aagtagaggt tattggagaa gtggaagcag tcgaagtgac ggaagcctac ttgtcagaaa
    26161 ttatttctgc acgaatcaag cacatccttg aacaaatcaa gcaagaatta gatagaaggc
    26221 gtctattgga cctccctggt ggtattgtct taatcggtgg gaatgccatt ttaccaggta
    26281 tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa gctttatgtt ccaaatcaag
    26341 ttggtatccg taatccagcc tttgcgcatg tgattagttt atcagaattt gcgggtcaat
    26401 taacagaagt taatcttttg gctcagggag cgataaaagg tgagaatgac ttaagtcatc
    26461 agccaattag ttttggtggg atgctgcaaa aaacagctca gtttgtacaa tcaacgcctg
    26521 ttcaaccagc tcctgctcca gaagtagagc cggtggcgcc tacagaacca atggcggatt
    26581 tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg tttccgtggc ttgatcggaa
    26641 gcatgtttga cgaataaaga ggaaaaataa attatgacat tttcatttga tacagctgct
    26701 gctcaagggg cagtgattaa agtaattggt gtcggtggag gtggtggcaa tgccatcaac
    26761 cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg cagcaaacac agatgtacaa
    26821 gcattgagta gtacaaaagc tgagactgtt attcagttgg gacctaaatt gactcgtggt
    26881 ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg ctgaagaaag cgaagaaaca
    26941 ctgacggaag ctattagtgg tgccgatatg gtcttcatca ctgctggtat gggaggaggc
    27001 tctggaactg gagctgctcc tgttattgct cgtatcgcca aagatttagg tgcgcttaca
    27061 gttggtgttg taacacgtcc ctttggtttt gaaggaagta agcgtggaca atttgctgta
    27121 gaaggaatca atcaacttcg tgagcatgta gacactctat tgattatctc aaacaacaat
    27181 ttgcttgaaa ttgttgataa gaaaacaccg cttttggagg ctcttagcga agcggataac
    27241 gttcttcgtc aaggtgttca agggattacc gatttgatta ccaatccagg attgattaac
    27301 cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag ggaatgctct tatgggtatt
    27361 ggtatcggta gtggagaaga acgtgtggta gaagcggcac gtaaggcaat ctattcacca
    27421 cttcttgaaa caactattga cggtgctgaa gatgttatcg tcaacgttac tggtggtctt
    27481 gacttaacct tgattgaggc agaagaggct tcacaaattg tgaaccaggc agcaggtcaa
    27541 ggagtgaaca tctggctcgg tacttcaatt gatgaaagta tgcgtgatga aattcgtgta
    27601 acagttgtcg caacgggtgt tcgtcaagac cgcgtagaaa aggttgtggc tccacaagct
    27661 agaactgcta ctaactaccg tgagacagtg aaaccagctc attcacatgg ctttgatcgt
    27721 cattttgata tggcagaaac agttgaattg ccaaaacaaa atccacgtcg tttggaacca
    27781 actcaggcat ctgcttttgg tgattgggat cttcgccgtg aatcgattgt tcgtacaaca
    27841 gattcagtcg tttctccagt cgagcgcttt gaagccccaa tttcacaaga tgaagatgaa
    27901 ttggatacac ctccattttt caaaaatcgt taagtaaatg aatgtaaaag aaaatacaga
    27961 acttgttttt cgagaagttg cagaggctag tctgagtgct catcgagaga gtggttcggt
    28021 ctctgtcatt gcagttacca agtatgtaga tgtaccgaca gcggaagcct tgcttccgct
    28081 aggtgttcat catatcggtg aaaatcgtgt agataagttt ctggaaaaat atgaagcttt
    28141 aaaagatcga gatgtgactt ggcatttgat tggtaccttg caaagacgta aggtgaaaga
    28201 tatcattcaa tacgttgatt atttccatgc attggactca gtaaagctag caggggaaat
    28261 tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa gtaaatattt ctaaagaaga
    28321 aagcaaacac ggtttttcga gagaggaact gctggaaatc ttgccagagt tagccagact
    28381 agataagatt gaatatgttg gtttaatgac gatggcacct tttgaggcta gcagtgagca
    28441 gttgaaagag attttcaagg cggcccaaga tttacaaaga gaaattcaag agaaacaaat
    28501 tccaaatatg cctatgaccg agttaagtat gggaatgagt cgtgattata aagaagcgat
    28561 tcaattcggt tccacttttg ttcgtatagg tacatcattt tttaagtagg agagaaccat
    28621 gtctttaaaa gatagattcg atagatttat agattatttt acggaggatg aggattcaag
    28681 tctcccttat gaaaaaagag atgagcctgt gtttactcca gtaaattctt cacaggaacc
    28741 ggctctccca atgaatcaac cttcacagtc ggctggtaca aaagagaaca atatcaccag
    28801 acttcatgca agacaacagg aattggcaaa tcagagtcag cgtgcaacgg ataaggtcat
    28861 tatagatgtt cgttatccta gaaaatatga ggatgcaaca gaaattgttg atttattggc
    28921 aggaaacgaa agtatcttga ttgattttca gtatatgaca gaggtgcagg ctcgtcgttg
    28981 tttggactat ttggatggag cttgtcatgt tttagctgga aatttgaaaa aggtagcttc
    29041 taccatgtat ttgttgacac cagtgaatgt tattgtaaat gttgaagata tccgtttacc
    29101 agatgaagat caacagggtg agttcggttt tgatatgaag cgaaatagag tacgataatg
    29161 atttttttaa ttcgtatgat ttataatgca gtggatattt actccctgat tttggtagcc
    29221 ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca gtttaggtcg ttggattgta
    29281 gcgttggtga aaccagtgct tgctcccttg caacgcctgc ctttacagat agcgggtctt
    29341 gatttatctg tttgggtcgc gattgttttg gttcgatttt taggagaaaa cctagtgcgt
    29401 tttctggcga tgataggatg aataaaggga tttatcagca tttctccata gaagatcgtc
    29461 catttcttga caagggaatg gaatggataa agaaggtaga agatagctat gctccttttt
    29521 taactccttt tatcaatcct catcaggaga agctattaaa gattttggcc aaaacctatg
    29581 gtcttgcttg tagcagtagt ggggaattcg tctcgagtga gtatgttcga gttttattat
    29641 acccagatta tttccaacca gagttttcag attttgaaat atctctccag gaaattgtgt
    29701 attccaataa atttgaacat ttaacgcatg ctaagatttt agggacagtc atcaatcaat
    29761 tagggattga acggaaactt tttggagata tcctagtaga tgaagaacgg gcgcagatta
    29821 tgattaatca gcagtttctt cttctctttc aagatggact aaagaaaatt ggtcgtatac
    29881 ctgtttcgct ggaggaacgt cctttcaccg agaaaataga taagctagaa cagtatcgag
    29941 aactggattt atctgtgtct agttttcgat tagatgttct tttatcaaat gttttgaaac
    30001 tatctaggaa tcaagcaaac cagttgattg aaaagaaact tgtccaagta aattatcatg
    30061 tggtagacaa atcagattac actgttcaag ttggagactt gattagtgtg agaaaatttg
    30121 gtcgcttgag attacttcaa gataagggac aaacgaaaaa agagaagaaa aaaataaccg
    30181 tccagttatt attaagtaag tgaggaatag aatgccaatt acatcattag aaataaagga
    30241 caagactttt ggaactcgat tcagaggttt tgatccagaa gaagtcgatg aatttttaga
    30301 tattgtggtt cgtgattacg aagatcttgt gcgtgcgaat catgataaaa atttgcgtat
    30361 taagagttta gaagagcgtt tgtcttactt tgatgaaata aaagattcat tgagccagtc
    30421 tgtattgatt gctcaggata cagctgagag agtgaaacag gcggcgcatg aacgttcaaa
    30481 caatatcatt catcaagcag agcaagatgc gcaacgcttg ttggaagaag ctaaatataa
    30541 ggcaaacgag attcttcgtc aagcaactga taatgctaag aaagtcgctg ttgaaacaga
    30601 agaattgaag aacaagagcc gtgtcttcca ccaacgtctc aaatctacaa ttgagagtca
    30661 gttggctatt gttgaatctt cagattggga agatattctc cgtccaacag ctacttatct
    30721 tcaaaccagt gatgaagcct ttaaagaagt ggttagcgaa gtacttggag aaccgattcc
    30781 agctccaatt gaagaagaac caattgatat gacacgtcag ttctctcaag cagaaatgga
    30841 agaattacaa gctcgtattg aggtagccga taaagaattg tctgaatttg aagctcagat
    30901 taaacaggaa gtggaaactc caactcctgt agtgagtcct caagttgaag aagagcctct
    30961 gctcatccag ttggcccaat gtatgaagaa ccagaagtag ctccagtgca tccgacaggt
    31021 ccaacaccag ctacagaaac tgttgattca gcaccgggat ttgaagcacc gcaagaatct
    31081 gttacaattt tataagaaat attctgagaa caatatctta tccttatatt cccagcgagc
    31141 aggagatggt gtgagtcctg cattccctat cgataagatt atcctctcaa actatcaagt
    31201 ctgaatctag taagatttga cgttccccac gttacgggat aagagggaga aagactaaaa
    31261 ctttttccga ataaaggtgg taccacgatt ttcgtccttt ttggaagtcg tggtttttaa
    31321 tttgttatta tttataaagg agataccatg aaactcaaag acacccttaa tcttgggaaa
    31381 actgaattcc caatgcgtgc aggccttcct accaaagagc cagtttggca aaaggaatgg
    31441 gaagatgcaa aactttatca acgtcgtcaa gaattgaacc aaggaaaacc tcatttcacc
    31501 ttgcatgatg gccctccata cgctaacgga aatatccacg ttggacatgc tatgaacaag
    31561 atttcaaaag atatcattgt tcgttctaag tctatgtcag gattttacgc accatttatt
    31621 cctggttggg atactcatgg tctgccaatc gagcaagtct tgtcaaaaca aggtgtcaaa
    31681 cgtaaagaaa tggacttggt tgagtacttg aaactttgcc gtgagtacgc tctttctcaa
    31741 gtagataaac aacgtgaaga ctttaaacgt ttgggtgttt ctggtgactg ggaaaatcca
    31801 tatgtgacct tgactcctga ctatgaagca gctcaaattc gtgtatttgg tgagatggct
    31861 aataagggtt atatctaccg tggtgccaag ccagtttact ggtcatggtc atctgagtca
    31921 gcccttgctg aagcagagat tgaataccat gacttggttt caacttccct ttactatgcc
    31981 aacaaggtaa aagatggcaa aggagttcta gatacagata cttatatcgt tgtctggaca
    32041 acgactccat ttactatcac agcctctcgt ggtttgacgg ttggtgcaga tattgattac
    32101 gttttggttc aacctgctgg tgaagctcgt aagtttgtcg ttgctgctga attattgact
    32161 agcttgtctg agaaatttgg ctgggctgat gttcaagttt tggaaactta ccgtggccaa
    32221 gaactcaacc acatcgtaac agaacaccca tgggatacag ctgtagaaga gttggtaatt
    32281 cttggtgacc acgttacgac tgactctggt acaggtattg ttcatacagc ccctggtttt
    32341 ggtgaggacg attacaatgt tggtattgct aataatcttg aagtcgcagt gactgttgat
    32401 gaacgtggta tcatgatgaa gaatgctggt cctgaatttg aaggtcaatt ctatgaaaag
    32461 gtagttccaa ctgttattga aaaacttggt aacctccttc ttgcccaaga agaaatctct
    32521 cactcatatc catttgactg gcgtactaag aaaccaatca tctggcgtgc agttccacaa
    32581 tggtttgcct cagtttctaa attccgtcaa gaaatcttgg acgaaattga aaaagtgaaa
    32641 ttccactcag aatggggtaa agtccgtctt tacaatatga tccgtgaccg tggtgactgg
    32701 gttatctctc gtcaacgtgc ttggggtgtt ccacttccta tcttctacgc tgaagatggt
    32761 acagctatca tggtagctga aactattgaa cacgtagctc aactttttga agaacatggt
    32821 tcaagcattt ggtgggaacg tgatgccaaa gacctcttgc cagaaggatt tactcatcca
    32881 ggttcaccaa acggcgagtt caaaaaagaa actgatatca tggacgtttg gtttgactca
    32941 ggttcatcat ggaatggagt ggtggtaaac cgtcctgaat tgacttaccc agccgacctt
    33001 tacctagaag gttctgacca ataccgtggt tggtttaact catcacttat cacatctgtt
    33061 gccaaccatg gcgtagcacc ttacaaacaa atcttgtcac aaggttttgc ccttgatggt
    33121 aaaggtgaga agatgtctaa atctcttgga aatactattg ctccaagcga tgttgaaaaa
    33181 caattcggtg ctgaaatctt gcgtctctgg gtaacaagtg ttgactcaag caatgacgtg
    33241 cgtatctcta tggatatctt gagccaagtt tctgaaactt accgtaagat tcgtaacact
    33301 cttcgtttct tgattgccaa tacatctgac tttaacccag ctcaagatac agtcgcttac
    33361 gatgagcttc gttcagttga taagtacatg acgattcgct ttaaccagct tgtcaagacc
    33421 attcgtgatg cctatgcaga ctttgaattc ttgacgatct acaaggcctt ggtgaacttt
    33481 atcaacgttg acttgtcagc cttctacctt gattttgcca aagatgttgt ttacattgaa
    33541 ggtgccaaat cactggaacg ccgtcaaatg cagactgtct tctatgacat tcttgtcaaa
    33601 atcaccaaac tcttgacacc aatccttcct cacactgcgg aagaaatctg gtcatatctt
    33661 gagtttgaaa cagaagactt cgtccaattg tcagaattac cagaagttca aacttttgct
    33721 aaccaagaag aaatcttgga tacatgggca gccttcatgg actttcgtgg acaagcacaa
    33781 aaagccttgg aagaagctcg taatgcaaaa gttatcggta aatcacttga agcacacttg
    33841 acagtttatc caaatgaagt tgtgaaaact ctactcgaag cagtaaacag caatgtagca
    33901 caacttttga tcgtgtctga gttgaccatc gcagaaggac cagctccaga agctgccctt
    33961 agcttcgaag atgtagcctt cacagttgaa cgtgctgctg gtgaagtatg tgaccgttgc
    34021 cgtcgtatcg acccaacaac agcagaacgc agctaccagg cagttatctg tgaccactgt
    34081 gcaagcatcg tagaagaaaa ctttgcggaa gcagtcgcag aaggatttga agagaaataa
    34141 gattgaaaag tctaggcaaa attcaatttg agaagaaaag acaactaatt ttatagtcta
    34201 ttaaacgcat tgtatcacgt ttttgaacac ctgatatgat gcgtttttta tttattttaa
    34261 aaatttgcga ggtatgactt tttatactca acaagaatca aagagaaact tagcaagcta
    34321 acagtagtaa gataaaatag gaatttgata tt
//
LOCUS       DAVGJJ010000010        34055 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000010 DAVGJJ010000000
VERSION     DAVGJJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.010"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            71..868
                     /locus_tag="VVE35_000468"
     CDS             71..868
                     /locus_tag="VVE35_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU3060719.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMCAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1016..1138
                     /locus_tag="VVE35_000469"
     CDS             1016..1138
                     /locus_tag="VVE35_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU3060720.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1160..2200
                     /locus_tag="VVE35_000470"
     CDS             1160..2200
                     /locus_tag="VVE35_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU3060721.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWMASGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALA
                     EKYLPKSVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2371..3645
                     /locus_tag="VVE35_000471"
     CDS             2371..3645
                     /locus_tag="VVE35_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU3060722.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYETQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3655..4533
                     /gene="truB"
                     /locus_tag="VVE35_000472"
     CDS             3655..4533
                     /gene="truB"
                     /locus_tag="VVE35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU3060723.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4774..5076
                     /locus_tag="VVE35_000473"
     CDS             4774..5076
                     /locus_tag="VVE35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU3060724.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5184..5822
                     /gene="udk"
                     /locus_tag="VVE35_000474"
     CDS             5184..5822
                     /gene="udk"
                     /locus_tag="VVE35_000474"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU3060725.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5950..7290
                     /locus_tag="VVE35_000475"
     CDS             5950..7290
                     /locus_tag="VVE35_000475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU3060726.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEEAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVVSSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7268..7480
                     /locus_tag="VVE35_000476"
     CDS             7268..7480
                     /locus_tag="VVE35_000476"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU3060727.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7477..8352
                     /locus_tag="VVE35_000477"
     CDS             7477..8352
                     /locus_tag="VVE35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU3060728.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8345..9160
                     /locus_tag="VVE35_000478"
     CDS             8345..9160
                     /locus_tag="VVE35_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU3060729.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9153..9584
                     /locus_tag="VVE35_000479"
     CDS             9153..9584
                     /locus_tag="VVE35_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3060730.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9591..11258
                     /gene="recN"
                     /locus_tag="VVE35_000480"
     CDS             9591..11258
                     /gene="recN"
                     /locus_tag="VVE35_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU3060731.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKVS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11260..11988
                     /locus_tag="VVE35_000481"
     CDS             11260..11988
                     /locus_tag="VVE35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU3060732.1"
                     /translation="MTDYYVIGDVHGKAEMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12067..13890
                     /gene="lepA"
                     /locus_tag="VVE35_000482"
     CDS             12067..13890
                     /gene="lepA"
                     /locus_tag="VVE35_000482"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU3060733.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAAEPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14049..14129)
                     /locus_tag="VVE35_000483"
                     /pseudo
     CDS             complement(14049..14129)
                     /locus_tag="VVE35_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14304..14780
                     /locus_tag="VVE35_000484"
                     /pseudo
     CDS             14304..14780
                     /locus_tag="VVE35_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14818..15108
                     /locus_tag="VVE35_000485"
     CDS             14818..15108
                     /locus_tag="VVE35_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3060734.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAK"
     gene            15128..15933
                     /locus_tag="VVE35_000486"
                     /pseudo
     CDS             15128..15933
                     /locus_tag="VVE35_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            15982..16446
                     /locus_tag="VVE35_000487"
     CDS             15982..16446
                     /locus_tag="VVE35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU3060735.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYIEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFIAFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEEIGFEIEVL"
     gene            16602..17027
                     /gene="lacA"
                     /locus_tag="VVE35_000488"
     CDS             16602..17027
                     /gene="lacA"
                     /locus_tag="VVE35_000488"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU3060736.1"
                     /translation="MAIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            17058..17573
                     /gene="lacB"
                     /locus_tag="VVE35_000489"
     CDS             17058..17573
                     /gene="lacB"
                     /locus_tag="VVE35_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU3060737.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            17584..18513
                     /locus_tag="VVE35_000490"
     CDS             17584..18513
                     /locus_tag="VVE35_000490"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU3060738.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            18515..19495
                     /gene="lacD"
                     /locus_tag="VVE35_000491"
     CDS             18515..19495
                     /gene="lacD"
                     /locus_tag="VVE35_000491"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU3060739.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            19729..20565
                     /locus_tag="VVE35_000492"
     CDS             19729..20565
                     /locus_tag="VVE35_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU3060740.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEYHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            20601..20918
                     /locus_tag="VVE35_000493"
     CDS             20601..20918
                     /locus_tag="VVE35_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3060741.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALEAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            20918..22609
                     /locus_tag="VVE35_000494"
     CDS             20918..22609
                     /locus_tag="VVE35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU3060742.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQQQFD"
     gene            22824..24230
                     /gene="lacG"
                     /locus_tag="VVE35_000495"
     CDS             22824..24230
                     /gene="lacG"
                     /locus_tag="VVE35_000495"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU3060743.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            24273..24530
                     /locus_tag="VVE35_000496"
     CDS             24273..24530
                     /locus_tag="VVE35_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU3060744.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDADLEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(24822..25583)
                     /locus_tag="VVE35_000497"
     CDS             complement(24822..25583)
                     /locus_tag="VVE35_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3060745.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKYIKVIKP"
     gene            complement(25765..26727)
                     /gene="nrdF"
                     /locus_tag="VVE35_000498"
     CDS             complement(25765..26727)
                     /gene="nrdF"
                     /locus_tag="VVE35_000498"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU3060746.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(26916..29075)
                     /gene="nrdE"
                     /locus_tag="VVE35_000499"
     CDS             complement(26916..29075)
                     /gene="nrdE"
                     /locus_tag="VVE35_000499"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU3060747.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(29156..29374)
                     /gene="nrdH"
                     /locus_tag="VVE35_000500"
     CDS             complement(29156..29374)
                     /gene="nrdH"
                     /locus_tag="VVE35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU3060748.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            29725..29988
                     /locus_tag="VVE35_000501"
     CDS             29725..29988
                     /locus_tag="VVE35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU3060749.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            29994..31727
                     /gene="ptsP"
                     /locus_tag="VVE35_000502"
     CDS             29994..31727
                     /gene="ptsP"
                     /locus_tag="VVE35_000502"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU3060750.1"
                     /translation="MTEMLKGIAASDGVAVAKVYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            31982..>34055
                     /gene="phtB"
                     /locus_tag="VVE35_000503"
     CDS             31982..>34055
                     /gene="phtB"
                     /locus_tag="VVE35_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU3060751.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQERS
                     HNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELS
                     ASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSLL
                     RELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIAR
                     IIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKV
                     GDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYN
                     KAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPE
                     RLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIK
                     KDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMP
                     YNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPN
                     ERPHSDNGF"
BASE COUNT        10451 a         6225 c         7484 g         9895 t
ORIGIN      
        1 tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa aaaagattta
       61 ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt gcaataagaa
      121 agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt gcgcaggtgc
      181 atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta ataaaattgc
      241 accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg cctacattgt
      301 ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg gtagtttctt
      361 aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct tgttcaacct
      421 tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg cgaatctgat
      481 acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa atgaattggt
      541 cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt catttatttt
      601 ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca tgtttgtatt
      661 cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga aattcagtgt
      721 tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact ggggtgtgac
      781 tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg ccttcctcaa
      841 taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg tgcttttttc
      901 attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact gatatttgta
      961 aactataact caaggtgcta caatatcctt aataaaataa tatggaggtc accttatgac
     1021 ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg ctccagctac
     1081 taagtctcgt gcagtgccga tttatcaaac aacatttttt gtttttgatg acacgtagga
     1141 aggtgccgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta tcaccaatcc
     1201 tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag gtatgactgc
     1261 agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg ctgcttcgac
     1321 tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg gtatcacaac
     1381 aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag acaataccaa
     1441 gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc tggaaaaact
     1501 ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt ttgcaatacc
     1561 ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg cgactaagtt
     1621 tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc ggtttgactg
     1681 gatggcttca gggaaattcc ctcaatttgt tgacgagggt tcaagctgcc acaatttgag
     1741 ctatactcgt gatgtgggtg cagtagcctt tattatagct gttcgagttc aattgcttcg
     1801 tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa gacttgaaac
     1861 cctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg attttcttgt
     1921 caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc cttattatgc
     1981 cttggctgag aaatacttgc caaaaagtgt cggttcaatc tttaccttcc atgtcaaagg
     2041 tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg accttgcaaa
     2101 cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg gtcaattgtc
     2161 agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt caatcggtct
     2221 tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa tttaaagtaa
     2281 aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg catgatataa
     2341 tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc caactgtggg
     2401 gaagtcttta cagtaaatga gagtcagtat gccgaactct tgtcccaagt gagaacggca
     2461 gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc tgagcaaaag
     2521 gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc gcaattacag
     2581 agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt tgaacagaca
     2641 agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa tcagttggct
     2701 accttgcgtt tggagcatga aaatcaacta caaaagaccc tttctgacct agaaaaagaa
     2761 cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt atctttggct
     2821 tctgttaagc aaaactacga aacccagctc aaggcagcta gtgaacaagt cgagttttat
     2881 aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga acagtatgca
     2941 gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt tgagaaggat
     3001 aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg tgatgaaaat
     3061 ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcagacgg aacagagaag
     3121 aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga gaagaactgt
     3181 gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa cacagggatt
     3241 gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt ctttatccag
     3301 ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca ggagttggcc
     3361 ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga tgcctttaag
     3421 ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc tattgatgaa
     3481 atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgaccac atctgaaaac
     3541 caactccgtt tagctaacaa caaattggaa gatgtctctg ttaaaaaatt gacccggaaa
     3601 aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac
     3661 ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt ttttaaactg
     3721 cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc ggatgtggtg
     3781 ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat gcaggacgag
     3841 ggtaagatct atgaggggga aattactctg ggttattcca cgacgactga ggatgctagt
     3901 ggggaagtga tcgcagaaac ccctgttttg tctcccttgg atgaaaagct tgttgatgaa
     3961 gcgattgcta gcttgactgg gcctattacc cagattcccc ctatgtattc ggcagttaag
     4021 gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa
     4081 cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga tggccaactt
     4141 gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac tttgtcagtt
     4201 gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg tactagtgct
     4261 gctggcttac aattagaaga cgctcttacc ttggaggaaa ttgctgaaaa agtagaggct
     4321 gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt caaagttttc
     4381 ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct agaccaaacg
     4441 gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga aaaacggggc
     4501 aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa atcgggtgat
     4561 agataacaat tgcttgataa aaccccatac taatagtaga atggttttgg gaattataat
     4621 attccaattg ttgcgagttg taggtactca aataatctat atagaaattt agaggtgtga
     4681 aatgaagcaa tttaaaattc tttcagataa atatttagag tccattacag gttctggtgg
     4741 gaacttaggc ccaggatttg gtgtgataat tccatgatgc gaaatgagtt tcgagaaagg
     4801 gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag tcacctgttt
     4861 cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat ggccaatttg
     4921 agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa gtctgtcatt
     4981 gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact ggctttcttg
     5041 cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc agagggaaga
     5101 ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata gaatagacga
     5161 cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga gtgacaggtg
     5221 gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt cctgatgaaa
     5281 agatttccat gattgagcat gattcatact acaaggatca gtctcatttg acctttgaag
     5341 agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg atgatcgagc
     5401 agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac tatacagagc
     5461 atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc gttgagggca
     5521 ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt tttgtggata
     5581 cggatgacga tgtgcgcatt attcgtcgta tcaagcgtga tatggaggag cgtggccgta
     5641 gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac caccagttta
     5701 tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc aataccgtgg
     5761 ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga aacagcaaat
     5821 aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg tacataaatg
     5881 gagattttta ggcatatttt tggtataata atacccatga aagagcaagt aaatgaatag
     5941 taggtggaga tggaaaagta tttatcggta acaactttga ccaagtatct gaaaatgaaa
     6001 ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc caactttcgt
     6061 aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat tcaagcgacc
     6121 atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat gaagatcaat
     6181 gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat cattgaaaaa
     6241 gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa gaaaaaattg
     6301 acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt ttctaagaga
     6361 attggggtag taaccagccg tagtggagcc gttattcgag atattatcac gaccgtcagc
     6421 aggcgattcc caggtgttga catccttctt tatccgacca aggttcaagg tgaagaggct
     6481 gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggatgattt ggatttgctc
     6541 attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga agaaattgtg
     6601 gtacgagcta tttttgaatc tcgtttgcca gttgtctcta gtgtggggca tgagacggat
     6661 gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc ggcggctgaa
     6721 ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca ggaaaaacgg
     6781 atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa aaaatgcagt
     6841 cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca acgcttggac
     6901 caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa caaacaatta
     6961 gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat ccaacgctat
     7021 caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt agtttatgac
     7081 gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga tactagccga
     7141 atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccattgtaga ttcggttgag
     7201 agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt agaattagag
     7261 gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg aaaccattgt
     7321 ccaaagtttg gaaaatggtg aaattgctct ggaagatgca attactgcct ttcaaaaggg
     7381 catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga ccttggtcaa
     7441 ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga aaaattagct
     7501 cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc tagtttacgg
     7561 gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt tctcttgtta
     7621 gaagttctgg aagccttgca ggttgccatc aaacctgctc acgcgcaggt agctactgcc
     7681 ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat ggatgatgac
     7741 gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat ggccattttg
     7801 gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga tttgccaagt
     7861 cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct gggtatggtg
     7921 gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga agaacttcag
     7981 actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc ggcagctatt
     8041 atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga attgattgga
     8101 cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga ggaaatcggc
     8161 aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt gttgggcttg
     8221 gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa attagaagaa
     8281 attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga aagtttgaga
     8341 atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt ttgaaacgag
     8401 agagcaggcc aagcgaggtg tgatggctgg tctagtcgta gcagtcctta atggagaacg
     8461 gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca agggggagaa
     8521 actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg tctttgattt
     8581 gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt ttaccgatgt
     8641 catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca atcagttggc
     8701 ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt ttcgctatgc
     8761 tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga gtttcatttc
     8821 ccttagtctg attttgccag ccttgcaccg tgtcttggct gatcaaggtc aggtggtagc
     8881 acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg gaattattcg
     8941 agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag tagaggcagg
     9001 tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa atattgaatt
     9061 tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg agattaaaga
     9121 agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga cttgaaaaaa
     9181 ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta gaacatttga
     9241 aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa gagttaggta
     9301 ttgtcaaaat tcctttgaga gacaacacct atgtctacga attaccaaaa tcaatcgtaa
     9361 aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat aagatgatca
     9421 atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc gatacttttg
     9481 cagacaagat ttttagttgt ttgactgatg atagctcgat tttagtcatt gccagaagtg
     9541 gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat atgttacttg
     9601 aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt gaaaagggga
     9661 tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat gccatgaata
     9721 tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca aaggcagaga
     9781 ttgaggggct tttctcagtt gagaatagtc gccttttaca ggaaattttt gatgagcaag
     9841 gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat ggtcgtagta
     9901 tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgagctatt gggcaacatc
     9961 ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa ctgcatatcc
    10021 agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc tatcaaacga
    10081 gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa aaccaacagg
    10141 aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag gcagcaaact
    10201 tgcaggctgg agaagacttg gctctcaatc aagagcgaga taaactcctc aaccacaaaa
    10261 atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat ttttcaagtc
    10321 tggccaatgt tcgttcagct atgaatgaca tggaaagtgt cgaagagtat gatcctgaat
    10381 accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac attagcaaac
    10441 gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctgatg caggttgaga
    10501 atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt gatgatgttt
    10561 tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat aatctttcgt
    10621 ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat ttggcaggtc
    10681 aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt aaacaagaac
    10741 tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag ggaaaattca
    10801 gtcgtgaggg aaataaaatg gttgagtttt atatttcaac caaccctgga gaagacttta
    10861 aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta gccattaagt
    10921 ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg gatacgggag
    10981 tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt cagcatggtc
    11041 aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat caattcttta
    11101 ttgagaaggt tagtaatgac cattcaacgg tttcgactgt tcgtctcttg acggtcgaag
    11161 agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa gcagccctga
    11221 cgcaagccag agaattgttg agaaacaggg agaaataaga tgacagatta ttatgtaatt
    11281 ggagatgttc acggaaaagc tgagatgctg gaagaccttc tcaaaacctg ggatggtcag
    11341 ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca ccgtgttctt
    11401 gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtctgg aaaccacgag
    11461 tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta tcgtcgcaat
    11521 ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc agttgatgga
    11581 gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt cattcgtcaa
    11641 atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat tgatttgacc
    11701 ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa accattccac
    11761 ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt ctatggtttg
    11821 ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa gattggaatg
    11881 gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga ccaaggaatg
    11941 acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta ctcctagcag
    12001 ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc tgaaaggaag
    12061 agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa cttctctatt
    12121 atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga aaaaacagag
    12181 acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggatct agagcgggaa
    12241 cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa agatggggaa
    12301 acttatattt tccacttgat tgacacacca gggcacgttg actttactta tgaagtttca
    12361 cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca aggaattgag
    12421 gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat catgcctatc
    12481 attaataaaa ttgacctgcc ggctgcagag ccggagcgcg tgcgtacaga gattgaagat
    12541 gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg tattgggatt
    12601 gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga tgtgacggcg
    12661 ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt tatcctccaa
    12721 gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat gagcaatagt
    12781 aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg tcgtgatttc
    12841 cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca ggatactcgt
    12901 gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca tggttataag
    12961 cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa gtacaatgac
    13021 ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt tgaaccagaa
    13081 acatctcagg cacttggatt tggttttcgt tgtggatttc ttggacttct ccatatggat
    13141 gttatccagg aacgtttaga gcgtgagttc aatattgatc tcatcatgac agctccgtct
    13201 gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa cccatctgag
    13261 tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc gcaaatcatg
    13321 gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg tggggacttt
    13381 gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat tcctcttgct
    13441 gaaattgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta tgcaagcttt
    13501 gactacgaat tgtcagaata tcgcccatct aagctggtga aaatggatat ccttctcaat
    13561 ggagacaagg tggatgccct cagctttatc gttcacaagg actttgccta cgaacgtggg
    13621 aaactcatcg ttgataaact caagaaaatc atccctcgcc aacaatttga agttccaatc
    13681 caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct tcgtaagaac
    13741 gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct tgaaaaacaa
    13801 aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca agaagccttc
    13861 ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc ttgttcacag
    13921 gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt ttagttttta
    13981 tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt atggttactg
    14041 acttcgtcag tttcatctac aacctcaaaa ctgtgttttg agtaacctgc ggctagcttc
    14101 ctagtttgct ctttgatttt cattgagtat tagaacagaa aaatgcttgg agtatttgtt
    14161 tgtgtgttta tttttatata acaaactata aacacaacaa aaatataaaa aaagagacaa
    14221 aaaaagaaca gaaagtaatt aacaacgatt tcatatagtg ttatacttat agcataaagt
    14281 taatggaaag aagggaaaac ctcatggtat tagattattt ctttgacaaa aaccttgtgt
    14341 tttgcttaga agcggataat caagaataac tctttgatca ggtagcaagc ttgttggagg
    14401 aacgagaaat tgtaactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc
    14461 caactggttt agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcata
    14521 cagatattgt tcataatcta gctgagaaag tggtggttgt tcgattagaa aaaccagtaa
    14581 cttttcacaa tatgatagct cctgataagg aagtagaagt atccctactc ttctttatta
    14641 ttaacaattc aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag
    14701 gaaatggaca tcttgaagat ttatcaaaaa tttccgaacc agaaaaactt tatgcttaca
    14761 ttgctgaagc aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatccaaatg
    14821 attaaaattc ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct
    14881 ctagaagaag aactttcaaa ccgtggttac gatgttcact gtgatgcagt tatggtgaaa
    14941 gatgtaaacg aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac
    15001 cttggttttg aaccaggtat cccagttatc gaagctgggc caatcttatt ccgtatccca
    15061 gcaatgagcg ttccagtatt tgacaatatc agacttcctg cgaaataaaa tatggtatag
    15121 tagttctatg aatgatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt
    15181 tggtgttcag cgtaccactt ttgaagagat gttagctgta ttaaaaacag cttatcaact
    15241 taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc ttatggccac
    15301 tcttcaatat gtgcgagaat atcgcactta tgaagaaatt gcggctgatt ttggtatcca
    15361 cgaaagcaac ttactccgtc ggagccaatg ggttgaagta actcttgttc aaagtggtgt
    15421 tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt
    15481 acaaatcaat cgccctaaaa aaagaattag cgaatgattc tggtaaaaag aaatgccacg
    15541 ctatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcgctg
    15601 tgaactattt gttatgatat gaagttgttc aaaatgagtc gcagaaatat cggacaagct
    15661 ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa
    15721 actccacgta aatccagcaa actcaagccg ctaacagttg aagataaagc ctgtaatcat
    15781 gcgctatcta aggagagaag caaggttgag aatatctttg ccaaagtaaa aacgtttaaa
    15841 atgttttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt
    15901 gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctaa taaagaaact
    15961 agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc tctacttgat
    16021 gatgaagtct acgaagatta ctatatagag tttgagaaag aagagacttg ctctgttcca
    16081 cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg gctagaggga
    16141 caaaaagaaa tagatcttag ttatgatctt ttcagcacag atgcagtgac tttggatgaa
    16201 ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataagggatt gaaagtgcat
    16261 tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc tttcattgcc
    16321 tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt agaagataag
    16381 aagaatgttt gtctcttaga agccaatcag gttgaagaaa tagggtttga gatagaagtt
    16441 ttataaaaaa gtaaaaaata aataacaaaa acaaacacaa aatattgact tcttcaaaca
    16501 atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca gccaatacac
    16561 tgctggaatc cagcatagaa aaataaaaag gagtatacaa tatggctatt gttatcggtg
    16621 cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga cttcttggaa aaggaaaact
    16681 tccaccttgt ggatgttaca gctgaaggtc aagactttgt tgatgtgact cttgctgtcg
    16741 ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt gattgatgct tatggagctg
    16801 gtccatttat ggtcgcaaca aaaatcaaag gaatggttgc tgcagaagta tctgacgaac
    16861 gttcagctta tatgactcgt ggccacaaca actcacggat gatcactatg ggtgcacaac
    16921 ttgttggtga tgaattggct aaaaacatcg ctaaaggatt tgttaacggt aaatacgatg
    16981 gtggacgtca tcaaatccgg gtcgacatgt tgaacaaaat gggctaatta gattacagta
    17041 agaaaggtaa gttaaaaatg agaattgcaa ttggatgcga ccacatcgta acagatgaaa
    17101 aaatggcggt ttcagaattt ttgaaatcaa aaggatatga agtcattgac tttggtacat
    17161 atgaccatac acgaactcac tacccaatct ttggtaaaaa agtaggggaa gctgtaacta
    17221 gcggccaagc tgatcttggg gtatgtatct gtggtactgg tgttggtatc aacaacgctg
    17281 taaataaagt tccaggtatt cgttctgcct tggttcgtga tatgacaaca gccctttatg
    17341 ctaaagaaca attgaacgcc aacgttatcg gttttggtgg taagattact ggtgaattgc
    17401 ttatgtgtga tatcatcgaa gctttcattc atgctgaata caaaccatct gaagaaaaca
    17461 aaaaattgat tgcgaaaatt gaacaccttg aaagtcacaa tgctcaacaa acagacgcaa
    17521 acttcttcac tgaattcctt gaaaaatggg atcgcggaga ataccacgac taagaggtaa
    17581 cctatgattt taacagttac aatgaaccca tccattgata tttcctatcc cttggatgag
    17641 ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca aaacggctgg tggtaaggga
    17701 ctcaatgtta cccgagtact ttcagaattt ggcgattctg ttcttgctac tggtttagtg
    17761 ggtggcaaac ttggtgagtt tttggttgaa catatcgata atcaagtaaa gaaagatttc
    17821 ttctcaattc agggagaaac tcgtaactgt atcgctattc tccacggaga caaccaaaca
    17881 gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag gtcaagactt tttggaacat
    17941 ttcaaaaaac tcttggagtc agttgaagta gtggccatct caggtagtct gccagctggc
    18001 cttccagttg attactatgc gagcttggta gaacttgcta atcaagctgg caagcctgta
    18061 gtcttggact gctcaggtgc agcacttcag gctgttcttg aatcacccca taaaccaaca
    18121 gtcatcaaac caaataatga agaattgtct cagcttcttg gaagagaagt ttctgaggat
    18181 ttggatgaat taaaagaagt acttcaagaa cctttgtttg cagggattga atggattatc
    18241 gtttcacttg gtgccaacgg tacttttgcc aaacatggtg acactttcta caaggtagat
    18301 attcctagaa ttcaggtggt aaatcctgtt ggatctggag actctactgt ggcaggaatt
    18361 tcttcaggac ttcttcacaa agaatcggat gcagaattac tcatcaaggc aaatgtcctt
    18421 ggtatgctca atgctcaaga aaaaatgact ggtcatgtca acatggccaa ctatcaagtt
    18481 ctatatgatc aattaatagt aaaagaggta taaaatggct ttaacagaac aaaaacgtgt
    18541 acgcttagaa aaactttctg atgaaaatgg tatcatctca gctcttgcat ttgaccaacg
    18601 tggtgctttg aaacgcctca tggctcaaca ccaaacagaa gaaccaactg tggcccaaat
    18661 ggaagaactt aaagtcttgg tagcagatga attgactaaa tacgcttcat ctatgcttct
    18721 tgaccctgag tatggacttc cagcaactaa agctcttgat gaaaaagctg gtcttctcct
    18781 tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa cgcttgccag actgcttgga
    18841 tgtctggtct gcaaaacgta ttaaagaaga aggtgcagat gcagttaaat tcttgcttta
    18901 ctatgatgta gatagctcag acgaactcaa tcaagaaaaa caagcctaca tcgaacgcat
    18961 cggttctgag tgtgtggctg aagatatccc attcttcctt gaaatccttg cttacgatga
    19021 aaaaattgcg gatgcaggtt ctgtagaata cgctaaagta aaaccacaca aagttatcgg
    19081 cgctatgaaa gtcttttcag acccacgctt taacattgat gttttgaaag ttgaagttcc
    19141 tgttaacatt aaatatgttg aaggcttcgc tgaaggtgaa gtagtttata cacgtgaaga
    19201 agcagcagcc ttcttcaaag cgcaagatga agcaacgaac ttgccataca tctacttgag
    19261 tgctggtgta tcagctaaac tcttccaaga tactcttgta tttgctcatg aatcaggtgc
    19321 aaactttaac ggagttcttt gtggccgtgc tacatgggca ggatcagttg aagcttacat
    19381 caaagatggt gaagcagcag ctcgcgaatg gcttcgcaca actggatttg aaaacattga
    19441 cgaactcaac aaagttcttc aaagaacagc aacttcatgg aaagaacgcg tgtaagaaag
    19501 tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa aaagttgtat gtaaaggttt
    19561 acaaaataac ttacttgtgc tatacttaaa tcacaagtta atacaaggtg agtgttacta
    19621 agtaatatta ggcatgatca caggtgaatt agaaatcagc tgattttcta gttcatttgt
    19681 ggtcattttt tgtacttata tacctttaag atataaaagg aggttgacat gtatcgaatt
    19741 ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg ataagggaga agaggtgatt
    19801 gtaattggta aggggattgc attcggaaag aagaaggggg atttgattgc tgaaaatcag
    19861 gttgagaaaa tctttcggat gaagaccgaa gagtccagag aaaactttat ggctcttctc
    19921 aaagatgttc cgcttgattt tatcacagtg acctatgaaa tcattgataa gctatcaaag
    19981 aaatatcatt atccgattca agagtatctc tatgtaacct tgacagatca tatttactgt
    20041 tcttatcaag ctctaactca aggaaggtac aaggatagta atctgccaga tatttccgct
    20101 aagtatcctg tcgcttttca aatcgcaaat gaagcttttg aaatttaccg tcagaagcta
    20161 gcagatcatt ttcctgagga cgaaattatt cggattgctt atcatttcat taatgctgaa
    20221 ggtgaaaatg aagtggaact tgtggagtcg attgataaga ggaaagaaat tctcaggaat
    20281 gttgaagaag ttttaacgga ctatgcaatt caacgaacta aaaagaataa ccatttctat
    20341 gatcgcttta tgatccattt gaattatttc ttggattatt tagacagatc tagagatgat
    20401 aaccaatcac ttctggatat ggaatatcat attaaacaat cctatccaaa agccttcgag
    20461 attggttcca agatctatga tgtgattacg caacatacgg gtcttgattt gtataaaagt
    20521 gaacgagttt atctagttct acatatccaa cgtttattgt cataaaaatt tatttaaaac
    20581 tatataagga gaattctatc atgaatagag aagaagtaac attgttaggt tttgaaatcg
    20641 tagcctatgc tggcgatgct cgttcaaaac tattggaagc cttggaggct gctgaagctg
    20701 gtgattttga aaaagcggac gctctggtag aggaagctgg tagctgtatt gcagaggctc
    20761 accacgcgca aacaagtcta ttgactaagg aagcttcagg tgaggacttg gcttatagtg
    20821 taaccatgat gcatggccaa gaccacttaa tgacaactat cttgttaaaa gatttgatgc
    20881 atcatttaat tgaactctac aagagaggag ttaaataatg aataaactaa ttgcatttat
    20941 cgagaaagga aagcctttct ttgaaaaact atctcgtaat atctatcttc gtgctattcg
    21001 tgatggtttc attgcaggta tgcctgttat tctcttctca agtatcttta tcttgattgc
    21061 ctttgtacca aactcatggg gctttaaatg gtctgatgaa gttgtagcct ttctgatgaa
    21121 accttatagc tattctatgg gtattctggc tctcttggta gctggtacaa cagctaagtc
    21181 attgactgac tcagtaaacc gtagcatgga aaaaaccaat caaatcaact atatgtcaac
    21241 attgttggca gcaattgttg gtttgttgat gttggcagct gatcctatcg aaagtggtct
    21301 agctactgga ttcttgggga caaaaggttt gctttcagcc ttccttgctg ccttcgttac
    21361 tgtagccatc tataaggttt gtgttaagaa caacgtcact attcgtatgc ctgacgaagt
    21421 tccaccaaat atctcacaag tctttaaaga tgtgattcca ttcactctct cagttgtttc
    21481 actttatgct cttgatttac tagctcgtca ttttgttggt gcaagcgtag ctgaatcaat
    21541 cggtaaattc tttgcaccac tcttctcagc agcagacgga taccttggta ttaccattat
    21601 ctttggtgcc tttgccttct tctggtttgt gggtattcat ggtccatcta ttgttgaacc
    21661 tgctatcgca gcaattacct atgcaaatgc tgaagtcaac ttgaaccttc tccaacaagg
    21721 tatgcacgct gacaagattc ttacttctgg tacacaaatg tttatcgtta ccatgggtgg
    21781 tactggtgcg acactggttg ttccattcat gttcatgtgg ttaacaaaat caaaacgtaa
    21841 ccgtgcaatc ggacgtgctt cagtagttcc taccttcttc ggtgtaaatg agccaatctt
    21901 gtttggtgca cctcttgttt tgaatccaat cttcttcatt ccatttatct ttgctccaat
    21961 tgcaaacgta tggatcttta aattctttat tgaaactctt ggaatgaact cattcactgc
    22021 taatctacca tggacaaccc cagctccact aggtctagtt cttggtacta acttccaagt
    22081 tctatcattc attcttgctg cccttctaat cgtggttgac gttgtcattt actatccatt
    22141 ccttaaggtc tatgatgaac aaattcttga agaagaacgt tcaggtaagt ctaatgatga
    22201 attgaaagaa aaagttgctg caaacttcaa cactgcaaaa gcggatgcta ttcttgaaaa
    22261 agcaggtgta gaagcagcac aaaatacaat cactaaagaa acaaatgtcc tcgttctttg
    22321 tgcaggtgga ggaacaagtg gtctccttgc aaatgctcta aacaaggcag cagcagaata
    22381 caatgtccct gtgaaagcag cagcaggtgg ctatggtgct caccgtgaaa tgttgccaga
    22441 gtttgatttg gttatccttg ctcctcaagt tgcttcaaac tttgaagata tgaaggcaga
    22501 aacagataag ctcggtatta aacttgctaa gacagaaggc gctcaataca tcaaattaac
    22561 tcgtgatgga aaaggtgccc tagcattcgt acaacagcaa ttcgattaag gctaggaact
    22621 gtgaaacagt ctcccatcgt tacggaaatc gctatggcga atttcctaat cgccttatta
    22681 attcgtcggt aaaaagatat cgtttttacc tcctcatgtc acaattcggt gacttggtac
    22741 aagaagtgag atggagaagc atggctcact gactcctctc ctctcacttt tactttattt
    22801 aaatcaagaa ataggtgaaa aaaatgacaa aaacacttcc aaaagacttt attttcggtg
    22861 gcgcaacagc tgcttatcaa gcagaaggtg ctacacatac tgatggaaaa ggaccagttg
    22921 cttgggataa atatcttgag gataactact ggtacactgc cgaaccagct agtgattttt
    22981 acaatcgata tccagttgac ctcaagctag cagaagagta tggtgtcaat ggtattcgaa
    23041 tttctattgc ttggtcacgt attttcccga ctggttacgg ccaagtaaat gctaaaggtg
    23101 ttgagtttta tcataattta tttgcagagt gtcacaaacg tcatgttgag ccttttgtaa
    23161 ctcttcatca ctttgacacg ccagaagctc tccactcaaa tggagacttc ttaaaccgtg
    23221 aaaatatcga acattttgta gactacgctg ccttctgttt tgaagaattt ccagaagtaa
    23281 actattggac aacctttaat gaaattggac caatcggtga tggtcaatat ttggttggga
    23341 aattccctcc aggtatccag tacgaccttg ccaaagtctt tcaatcacac cacaatatga
    23401 tggtgtctca tgcacgcgcg gtaaaattgt acaaagagaa aggctataaa ggggaaattg
    23461 gtgttgttca cgccctgcct actaaatatc ctctagatca tgaaaatcca gcagatgttc
    23521 gtgcagctga gttggaagat attattcaca acaaatttat tttggatgca acctatctgg
    23581 gtcactactc tgaaaagact ctggcagggg tagaggctat cttggctgcc aatggtggta
    23641 gcttggatct tcgtgaagaa gattttacag cattagaagc tgcaaaagac ttgaatgatt
    23701 tcctaggaat caactactat atgagtgact ggatggaagc ctttgatgga gaaactgaaa
    23761 ttatccataa tggtaaaggt gaaaaaggaa gctctaaata ccaaatcaaa ggtattggtc
    23821 gtcgtgtagc tcctgactat gtaccacgca cggattggga ttggattatc taccctcaag
    23881 gtttgtatga ccaaatcatg cgcgtgaaga aagattatcc taactataag aaaatctaca
    23941 tcactgaaaa tggtcttggc tataaagatg agttcgttga taacactgtt tacgatgatg
    24001 gtcgtattga ttacgtgaaa caacacttgg agatcttgtc tgatgcgatt gcagatggag
    24061 ctaatgtaaa aggttacttc atttggtcat taatggatgt cttctcatgg tcaaacggtt
    24121 atgagaaacg ttatggtctc ttctacgtag attttgaaac tcaagaacgt tatcctaaga
    24181 aatcagctca ctggtacaag aaagtagcgg aaactcagat tatagactag tagaattagt
    24241 cattagatat agaattttag tgagtcaaaa agatgttcaa agattttatc caatctattt
    24301 atgaaaaagt ttatattata aatttcgaaa aatgctctca aataccgtgt ttgacgagtg
    24361 aggaattgaa aagtcttgga aaatggtatg tctcgactgg taaagaatgg atttgtcatt
    24421 cagatgctga tctggaagaa tttaaaaatc tatttttaaa ttttatcaat cctgaagaat
    24481 gggatactat ctcctttgat tcagatttta tgccgtttca acaatcgtaa ccaatttctc
    24541 aaaaaagtta aatcttatat ttagtactct gtaaaactct tatctaatca cgttgcttat
    24601 actcaatgaa aatcaaagag caactttaaa ctaggaagcg agtcgcagat ttctcaatgc
    24661 atagctttga ggtggcagat aaagcaattg ggcaaaaagt ctttgataca gaaaaaagca
    24721 tagtatcagg tgtttcaaca cctgatacta tgcgttttat tgtgggaaga tttacttttt
    24781 ttcttctgaa attgagttgt tactcaggct ctttcagttt attaaggctt gatgacttta
    24841 atgtatttag atagcttaaa aaggattgaa tcacttagtt tagaatctga aacaatagta
    24901 tcaagatttg atacattata aaaagtataa aaatcaaact tattgaactt actatgatct
    24961 gcgagtaaat attttttatt agaattattt aaagcgatgc gttgagcctc tccctcttcc
    25021 tcgctaaaag tagctagagc tccgttttga ataccattac agctaacgaa agctttagaa
    25081 aattggagat tagagagatt ttgtagggtc aatgtgccaa caaaagcacc tgtaatatcg
    25141 cgataatttc cacctatcaa aatcaaatct gttaattttc gttcgcttaa aatcagaaaa
    25201 acaggtagac tgttggttac gacgcggata ttgtcaatag gcaactcacg cgcaaaaaac
    25261 tctaatgttg ttcctggtcc aatgaaaata gtttctcttt cttctactag actgcctgca
    25321 aaatgggcta tttcttgttt ttctgccgtt tggagggctt gtttttcaat atttgatcgc
    25381 tcattagtca aaagggagtt ggttcgaagt ttttcagctc caccatgcac acgaatcagc
    25441 aaatctttat cagctaattc ctgtaaatag cgccttgcag tcatatctga aacggctatt
    25501 tcgtccataa tctgtttaac tgttatggtt cctttactat ttactatctc taaaattttg
    25561 gttaattttt cttgtttgag catattatca cctcgtttcc tactactatc ttaccataaa
    25621 caaactcatc attcaaatac aaaaacaaca aaatgaaaca aaaacaaaaa tatcgaagtt
    25681 tgttttcaaa actttcgata tttttgttgg gttataactt tgatgtttct agtttacttt
    25741 ttgatgattg agagtgatgg agaattagtc taaaccgtag ttatagtcat cgtcttgcat
    25801 ggcttcaact tcgccaagaa ggtaaccatt tccgacttga gagaagaagt catggttgga
    25861 agttcctgtt gaaataccgt tcataacgat tgggttgaca tcttcagctg aatctgggaa
    25921 aagtggatct tgtcccatgt tcatgagagc tttattggca ttgtagcgaa ggaaggtttt
    25981 aacctcttca gtccaaccaa caccgtcata aagactctct gtgtagcctt cttcattttc
    26041 ataaagagta tagagtaggt cgtacatcca ttctttgagt ttttcttgct cttcttcagg
    26101 caattcattg aaaccaagtt ggaatttgta accaatgtag gttccgtgaa cagactcgtc
    26161 acgaataatc aatttaatga tttctgcaac gttggctagt ttgttgttac cgagatagta
    26221 gaggggagtg aagaaaccag agtagaagag gaaggtttcg aggaagacgc tggcaacttt
    26281 cttttcaagt gggctgccgt ttaggtagat ttcgttgaca atctcagcct tcttttgtag
    26341 gtaaggattg gtattggtcc attcgaaaat ttcttcaatc tcagccttag tattcaaggt
    26401 agaaaagatt gatgagtaag atttagcgtg gacagattcc ataaattgga tgttattgaa
    26461 aacagcttcc tcatgtggtg tacggatgtc tgcgcgaagg gcttgaaccc cagtttcaga
    26521 ttgcatagtg tcaagaaggg ttaaaccacc aaaaactttt ccgaccaagt ctttctcttt
    26581 gttagatagc tttctccagt catccaagtc gtttgataag ggaatacgtg tatcgagcca
    26641 aaattgctcc gtcagttttt cccaagttga tttgtcgatg acatcttcga tggcattcca
    26701 gttaatggct ttgtagtaag tttccattta aaatctcttt ctgtgtttag tattgcgaac
    26761 tcacaattat ttctacttta ccataattct ataggagtat cgcacaaaaa gtcggaagcc
    26821 cgacttttaa aatgttacat aaattatgtt atgacatagt agatttgatt ttatctgtgc
    26881 tgcttaggga aaaataatgt ttctatagct agaaactaaa tcacacagct ttcacattgg
    26941 ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat agacttgatt
    27001 cccttgttaa aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg tttattttcc
    27061 ctcttccatt cgtaaaggcc ttttggaatg tcactacgca tgaagagggt gagtgaaagt
    27121 ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg catatccata
    27181 tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc agggtagtag
    27241 attttaccaa ttttcttctc ttggcgttct tcgatacgtt gcgtaatcgg gtggatagaa
    27301 gcagaaacgt cgttgatata gctgatagaa ccatttggcg ctacagcaag gcgattttgg
    27361 tggtaaagac catcttcttg aaccttgtcg cgaagttcag cccaatcagc aacaccaggg
    27421 ataaagacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa ttcaccagtt
    27481 acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt gtggaaggta
    27541 ataccacgtt cacgcgcaat attgtttgac tctaccaagg tccagtagtt cataagcata
    27601 aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag ttgttgggca
    27661 aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct atttccatgg
    27721 tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag ggcacgaacc
    27781 atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac gttggttgaa
    27841 cccaggttac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc gttgatcaag
    27901 cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat ctttccatca
    27961 acaggatttg cacggttagc cgtatctatg ttgactacat aaggatagcc agactcttgt
    28021 tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt gcgaatattt
    28081 ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa tggcacaccg
    28141 tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt acgagccaat
    28201 tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg tactttttca
    28261 tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa gacgttgagg
    28321 tagacaacac cggcaccttg acgttgcccc aattggttgg agtaagagaa gctgtcttca
    28381 aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt gataggtgca
    28441 ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg tgaaagttga
    28501 agagctgagt tgatagaacg tccgatagag ttcatatcat ccgtcacttg gattaggaaa
    28561 caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg agtagcaggt
    28621 tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc attcccatca
    28681 gcgaaataaa gggcgttgaa gaagacacgg tcttccatat tttcaagata gtattcaccg
    28741 tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa tgacttgaat
    28801 tggaagtttt ggtctttgat aaattgagcc aattcttcca agaactctgg acggtatttc
    28861 ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt gtctttgatt
    28921 gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc caaggcttcc
    28981 ttatctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt attaagacgg
    29041 aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc taattacaaa
    29101 agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac taagattatg
    29161 ctaattgttt cagttttcct ggttggaaac ctgaaaagac ttcagttggt gtttggataa
    29221 caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt tgctcatcaa
    29281 gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc attttacatt
    29341 ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc aaaatttaat
    29401 actatcttag tatatcagaa aataaaattt tgtcaatgat ttaaactaaa tatagtggtg
    29461 ttcttttgtt gtttttatat ataaacacaa tatatagtgt ttgtattgtg aaatagcaat
    29521 gattatccta taaattgttt ttgaagaaac tatatcctgt gttttgttat ctaggataga
    29581 agattttcag caagttatct gaaaggaaat tgaaggaaaa tccagaaaat gcttgaaaaa
    29641 aatcctagaa gatggtataa tactaaattg taagggttat cacatataac tcaaaaaaag
    29701 aaagaacaaa aggagagtca aactatggct tctaaagatt tccacgtagt ggcagaaaca
    29761 ggtattcacg cacgtccagc aacattgttg gtacaaactg ctagcaaatt tgcttcagat
    29821 atcactcttg agtacaaagg taaatcagtt aaccttaaat caattatggg tgttatgagt
    29881 cttggtgttg gtcaaggtgc tgacgtaact atctcagctg aaggtgcaga tgcagatgac
    29941 gctatcgctg caatctcaga aacaatggaa aaagaaggat tggcataagg gaaatgacag
    30001 aaatgcttaa aggaatcgca gcatctgacg gtgttgcagt tgcaaaagta tatctactcg
    30061 ttcagccgga tttgtcattt gagactatta cagtcgaaga tacaaacgca gaagaagctc
    30121 gccttgatgc cgctctacag gcatcacaag acgagctttc tgttattcgc gagaaagcag
    30181 taggtacgct cggtgaagaa gcagctcaag tttttgatgc tcacttaatg gttcttgctg
    30241 acccagaaat gatcagccaa atcaaggaaa ctatccgtgc gaagaaagtg aatgcagaag
    30301 caggtctgaa agaagttaca gatatgttta tcactatctt tgaaggcatg gaagacaacc
    30361 catacatgca agaacgcgca gcggatatcc gcgacgtgac aaaacgtgta ttggcaaacc
    30421 ttcttggtaa aaaattgcca aacccagctt ctatcaatga agaagtgatt gtgattgcgc
    30481 atgacttgac tccttcagat acagctcaat tggacaaaaa ctttgtaaaa gcttttgtaa
    30541 ccaacattgg tggacgtaca agccactcag ctatcatggc acgtacactt gaaattgctg
    30601 ctgtattagg tacaaacaac atcactgaaa tcgttaaaga cggtgacatc cttgctgtta
    30661 acgggatcac tggagaagtg attatcaacc caacagatga acaagcggca gaatttaaag
    30721 cagctggtga agcctatgcg aaacaaaaag ctgaatgggc acttttgaaa gatgctcaaa
    30781 cagtgactgc tgacggtaaa cacttcgagt tggctgccaa tattggtact ccaaaagacg
    30841 ttgaaggtgt taacaacaac ggtgcagaag ctgttggact ttaccgtaca gagttcttgt
    30901 acatggattc tcaagacttc ccaactgaag atgagcagta tgaagcatac aaggctgttc
    30961 ttgaaggaat gaacggtaaa cctgttgtcg ttcgtacaat ggatatcggt ggagataagg
    31021 aacttcctta cttcgatatg cctcacgaaa tgaacccatt ccttggattc cgtgctcttc
    31081 gtatctctat ctctgagact ggagatgcta tgttccgcac acaaatccgt gctcttcttc
    31141 gtgcgtctgt tcacggtcaa ttgcgtatca tgttcccaat ggttgcgctc ttgaaagaat
    31201 tccgtgcagc gaaagcagtc tttgatgaag aaaaagcaaa ccttcttgct gaaggtgttg
    31261 cagttgcgga taacattcaa gttggtatca tgatcgagat tcctgcagcg gctatgcttg
    31321 cagaccaatt tgctaaagaa gttgacttct tctcaattgg tacaaatgac ttgatccaat
    31381 atacaatggc agcagaccgt atgaacgaac aagtttcata cctttaccaa ccatacaacc
    31441 catcaatcct acgcttgatt aacaatgtga tcaaagcagc tcacgctgaa ggtaaatggg
    31501 ctggtatgtg tggtgagatg gctggtgacc aacaagctgt tccacttctt gtcggaatgg
    31561 gcttggatga gttctctatg tcagcaacat ctgtacttcg tacacgcagc ttgatgaaga
    31621 aactcgacac agctaagatg gaagagtacg caaaccgtgc ccttacagaa tgctcaacaa
    31681 tggaagaagt tcttgaactt caaaaagaat acgttaattt tgattaatcg aaaagtccct
    31741 gcaactcagt tacagggact ttttttgata ttttaaaaag aattttcaag aaaatctttc
    31801 ttataaaaag tccaaccttg aaaaagtagt ggtcagaaca aaaaatactt aaatggttca
    31861 taaaattctt gacaagttgg agatttagga gtaaactatt aaccagttaa gtaatagaga
    31921 ggagtttctg caatttagaa atgaattgca actagaaata tcaaatagaa agagagtttc
    31981 gatgaaaatt aataagaaat accttgttgg ttctgcggca gctttgattt taagtgtttg
    32041 ttcttacgag ttgggactgt atcaagctag aacggttaag gaaaataatc gtgtttccta
    32101 tatagatgga aaacaagcga cgcaaaaaac ggagaatttg actcctgatg aggttagcaa
    32161 gcgtgaagga atcaatgctg agcaaatcgt catcaagata acagaccaag gctatgtcac
    32221 ttcacatggc gaccactatc attattacaa tggtaaggtt ccttatgatg cgattttcag
    32281 tgaagaatta ctcatgaaag atccgaatta tcagttgaag gattcagaca ttgtcaatga
    32341 aatcaagggt ggttatgtca ttaaggtaaa cggtaaatac tatgtttacc ttaaggatgc
    32401 agctcatgcg gataatattc ggacaaaaga agagattaaa cgtcagaagc aggaacgcag
    32461 tcataatcat aactcaagag cagataatgc tgttgctgca gccagagccc aaggacgtta
    32521 tacaacggat gatgggtata tcttcaatgc atctgatatc attgaggaca cgggtgatgc
    32581 ttatatcgtt cctcacggcg accattacca ttacattcct aagaatgagt tatcagctag
    32641 cgagttagct gctgcagaag cctattggaa tgggaagcag ggatctcgtc cttcttcaag
    32701 ttctagttat aatgcaaatc cagctcaacc aagattgtca gagaaccaca atctgactgt
    32761 cactccaact tatcatcaaa atcaagggga aaacatttca agccttttac gtgaattgta
    32821 tgctaaaccc ttatcagaac gccatgtgga atctgatggc cttattttcg acccagcgca
    32881 aatcacaagt cgaaccgcca gaggtgtagc tgtccctcat ggtaaccatt accactttat
    32941 cccttatgaa caaatgtctg aattggaaaa acgaattgct cgtattattc cccttcgtta
    33001 tcgttcaaac cattgggtac cagattcaag accagaacaa ccaagtccac aatcgactcc
    33061 ggaacctagt ccaagtctgc aacctgcacc aaatcctcaa ccagctccaa gcaatccaat
    33121 tgatgagaaa ttggtcaaag aagctgttcg aaaagtaggc gatggttatg tctttgagga
    33181 gaatggagtt tctcgttata tcccagccaa ggatctttca gcagaaacag cagcaggcat
    33241 tgatagcaaa ctggccaagc aggaaagttt atctcataag ctaggagcta agaaaactga
    33301 cctcccatct agtgatcgag aattttacaa taaggcttat gacttactag caagaattca
    33361 ccaagattta cttgataata aaggtcgaca agttgatttt gaggctttgg ataacctgtt
    33421 ggaacgactc aaggatgtct caagtgataa agtcaagtta gtggatgata ttcttgcctt
    33481 cttagctccg attcgtcatc cagaacgttt aggaaaacca aatgcgcaaa ttacctacac
    33541 tgatgatgag attcaagtag ccaagttggc aggcaagtac acaacagaag acggttatat
    33601 ctttgatcct cgtgatataa ccagtgatga gggggatgcc tatgtaactc cacatatgac
    33661 ccatagccac tggattaaaa aagatagttt gtctgaagct gagagagcgg cagcccaggc
    33721 ttatgctaaa gagaaaggtt tgacccctcc ttcgacagac catcaggatt caggaaatac
    33781 tgaggcaaaa ggagcagaag ctatctacaa ccgcgtgaaa gcagctaaga aggtgccact
    33841 tgatcgcatg ccttacaatc ttcaatatac tgtagaagtc aaaaacggta gtttaatcat
    33901 acctcattat gaccattacc ataacatcaa atttgagtgg tttgacgaag gcctttatga
    33961 ggcacctaag gggtatactc ttgaggatct tttggcgact gtcaagtact atgtcgaaca
    34021 tccaaacgaa cgtccgcatt cagataatgg ttttg
//
LOCUS       DAVGJJ010000011        31445 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000011 DAVGJJ010000000
VERSION     DAVGJJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.011"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(645..1217)
                     /locus_tag="VVE35_000504"
     CDS             complement(645..1217)
                     /locus_tag="VVE35_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060752.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(2017..2865)
                     /locus_tag="VVE35_000505"
     CDS             complement(2017..2865)
                     /locus_tag="VVE35_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU3060753.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            3123..3401
                     /locus_tag="VVE35_000506"
     CDS             3123..3401
                     /locus_tag="VVE35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060754.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            3403..5466
                     /locus_tag="VVE35_000507"
     CDS             3403..5466
                     /locus_tag="VVE35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU3060755.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(5544..6800)
                     /locus_tag="VVE35_000508"
     CDS             complement(5544..6800)
                     /locus_tag="VVE35_000508"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU3060756.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            6942..9407
                     /locus_tag="VVE35_000509"
     CDS             6942..9407
                     /locus_tag="VVE35_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU3060757.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNRGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSLRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            9472..10383
                     /locus_tag="VVE35_000510"
     CDS             9472..10383
                     /locus_tag="VVE35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU3060758.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            10613..11311
                     /gene="dapD"
                     /locus_tag="VVE35_000511"
     CDS             10613..11311
                     /gene="dapD"
                     /locus_tag="VVE35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU3060759.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNVVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            11379..12509
                     /locus_tag="VVE35_000512"
     CDS             11379..12509
                     /locus_tag="VVE35_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU3060760.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTQTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            12521..13060
                     /locus_tag="VVE35_000513"
     CDS             12521..13060
                     /locus_tag="VVE35_000513"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU3060761.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERLLQHLFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVNVSKIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            13044..13721
                     /locus_tag="VVE35_000514"
     CDS             13044..13721
                     /locus_tag="VVE35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU3060762.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTTGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(13793..14692)
                     /gene="galU"
                     /locus_tag="VVE35_000515"
     CDS             complement(13793..14692)
                     /gene="galU"
                     /locus_tag="VVE35_000515"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU3060763.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKSKTDLLKLVDEATGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(14714..15730)
                     /locus_tag="VVE35_000516"
     CDS             complement(14714..15730)
                     /locus_tag="VVE35_000516"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU3060764.1"
                     /translation="MKKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSIILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDTIYDIMNNEFKAE
                     NEWS"
     gene            15942..16323
                     /locus_tag="VVE35_000517"
                     /pseudo
     CDS             15942..16323
                     /locus_tag="VVE35_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16497..16922)
                     /locus_tag="VVE35_000518"
     CDS             complement(16497..16922)
                     /locus_tag="VVE35_000518"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3060765.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(16990..17640)
                     /gene="phoU"
                     /locus_tag="VVE35_000519"
     CDS             complement(16990..17640)
                     /gene="phoU"
                     /locus_tag="VVE35_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU3060766.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(17655..18407)
                     /gene="pstB"
                     /locus_tag="VVE35_000520"
     CDS             complement(17655..18407)
                     /gene="pstB"
                     /locus_tag="VVE35_000520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3060767.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(18409..19224)
                     /gene="pstA"
                     /locus_tag="VVE35_000521"
     CDS             complement(18409..19224)
                     /gene="pstA"
                     /locus_tag="VVE35_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU3060768.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(19217..20080)
                     /gene="pstC"
                     /locus_tag="VVE35_000522"
     CDS             complement(19217..20080)
                     /gene="pstC"
                     /locus_tag="VVE35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU3060769.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(20198..21073)
                     /locus_tag="VVE35_000523"
     CDS             complement(20198..21073)
                     /locus_tag="VVE35_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU3060770.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(21240..22571)
                     /locus_tag="VVE35_000524"
     CDS             complement(21240..22571)
                     /locus_tag="VVE35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU3060771.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGEIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(22572..23279)
                     /locus_tag="VVE35_000525"
     CDS             complement(22572..23279)
                     /locus_tag="VVE35_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3060772.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(23363..23848)
                     /locus_tag="VVE35_000526"
     CDS             complement(23363..23848)
                     /locus_tag="VVE35_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU3060773.1"
                     /translation="MAPTDKEDLIRTLELTPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADGIWYFHAGSPLTVHMITADGHYEVVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            23834..24393
                     /locus_tag="VVE35_000527"
                     /pseudo
     CDS             23834..24393
                     /locus_tag="VVE35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24708..26399)
                     /locus_tag="VVE35_000528"
     CDS             complement(24708..26399)
                     /locus_tag="VVE35_000528"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU3060774.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            26535..26981
                     /gene="argR"
                     /locus_tag="VVE35_000529"
     CDS             26535..26981
                     /gene="argR"
                     /locus_tag="VVE35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3060775.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTVLVICRDQHVAKLMEDRLLDLMKDK"
     gene            27032..29566
                     /gene="mutS"
                     /locus_tag="VVE35_000530"
     CDS             27032..29566
                     /gene="mutS"
                     /locus_tag="VVE35_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU3060776.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQTLAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            29713..31407
                     /gene="patA"
                     /locus_tag="VVE35_000531"
     CDS             29713..31407
                     /gene="patA"
                     /locus_tag="VVE35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU3060777.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEVIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         9054 a         5982 c         6867 g         9542 t
ORIGIN      
        1 taataagctt gttacctgat aagatttttg gaaaaaataa aaaaatttgg aaaatagttt
       61 ttgcaatatt gacggcagtg gcagcattgt catttatgta ctaagttatt ttaagaatgt
      121 agggaaataa accctacatt ctttttagtt ttttctgttt tctaaattct atttatccaa
      181 gcgattcaac atttcttgct tcttcgcttc aagttctgca cgcttttctt cgatttcggc
      241 atgtttttta tcgagttcag aacaacttgc atcattgcta aattcttttc gccatcagga
      301 gatagggtga gtcgacatgt ctattactca cccaaagcag tcctacaaag caggaatttt
      361 ctgttacttt tttggaaata gtaacgttta tacagctttg acacttcgta tcaaagcgcc
      421 aaacacactc cgagggattt acagaaagca gaaaaggaat gatctggtat aagatcattc
      481 cttttctctc tttttcttta agtaattata tacaatgtac gacgaagtcg tcattgcaat
      541 gctgatccac cacctaaagg gaactttaaa caacattgat aagataaaga atataaacaa
      601 cgaaaatacg ttatacccaa ttaattttat tgtatatctc atgattaaaa gttaatcctt
      661 ccgttgttag gaatggcatc atttttatcc cataattgtg ctaaataagt ccccggtgat
      721 aataaattca tagcggattc taaagcaaca tcatttacaa accaactacc tagatatcta
      781 gaaattgctg aacgaatagc actttttgct gcatgttttc cttttacttt aattagattt
      841 gcaaggcctg cagtagttcc tcctaatgct aaagctattg tagtatctaa tagagcaccc
      901 atttgattaa ctgtaatacc ttgccaagct gctctaaatg gagagtatgt aggtgggatt
      961 gtataatcgc cttgtaattg tcggttaatt acttctttga tccattgttg tgagacgtct
     1021 ggatgaaaag attggatttc gtttgcaagt gtattgattt gttcttctgt tagagaagtg
     1081 acaggttgaa gttccatatt tgtttcaatt tgcgacactt gttcagaagc gtatacagct
     1141 gaaacacttg gaatcgctga tacaattaac acaattgacg tcaaaaaaac cgaaataaat
     1201 ttcattaatt tgttcatgag cttttctcct ttttatttgt atctgcttac attttattat
     1261 atactgttat tatagtcaag aaaaatatgc tattatgtta aaaaaatatt tttcaaaata
     1321 taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa
     1381 ccatgatttg tttactctca atcatcaaga attctctttt cgtggtagcg tttggggtct
     1441 ggtactggcc ttatatcact tattgttcat tgataagttt gtaatttcga atcgaaaata
     1501 aagattagag ctatgcttga ctgtgtactt ttaggattga ttttggagga agattttgtc
     1561 tctattattt attattttaa atttatttat tttgtataag atctattctt tgaggcggga
     1621 aaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg
     1681 tattcaatgg ttattaaaag aactgatttc aactatttcc ccttaatgcg tatggaaact
     1741 ataacaaaac gcataagatt aaggtttgtg tacctgttct tatacgtttt tgtcatattg
     1801 aagattttcg gtaagttttc tacttttgcg aagtctagtt tatactcaat gaaaatcaaa
     1861 gagtaaacta gccgtaggtt gctcaaagta cagctttgag gttgcagata aaactgacga
     1921 agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
     1981 gtacggcaag gcgacgttga cgtggtttga agagtattag aatgctttcc caaccaaaat
     2041 ctctgcttcg atggtaatct ctgtcagttg gctccagtca atcttgctct ggtcaacgtg
     2101 aaagaggaga ggagtcatac tgagtagggc ttggagttgt tctgctgtga tagtcttagt
     2161 cagagaggca gtttgactag ataggatggt aaagtgttct tggaaatgct ccttgatatc
     2221 ttggttagaa tactccttgt ttgtcagctg gtcctgtact ctttgacgga tttctttgag
     2281 gtgattttca gttgggataa cctttatcaa gataccgtct ttggataaaa cgcgacgaaa
     2341 ttctccatag ttggcaggtg agaagatatc aagcagaata tccatgttag cgtcttttat
     2401 aggaaggcgt gccaagtcgc caacgaacca attgactgcc cagttgggtt cacttttagc
     2461 agcgatttgg actgaatctt tggagatgtc aaaggcatag aaagtttttt cagagtgact
     2521 ttcttgtagt ttgcgagaat agaatccttc accacaaccg atatccaaaa ttgttgtggt
     2581 agtttttgag cttgcaagca agtcagatac agcatctaag atagcttggt aaaagccggc
     2641 ttctaggatt tgttgacggt tttgaaaatt ttccttgtca tagttagcgg attgcttgat
     2701 ttgaggtgct agattgacat agccaaattt cgccaagtca aaagaatgac ggttgcagca
     2761 cttgaaatta gtctctaaca gagtcagatt ttcttgacag ataggacagg caaaggcagt
     2821 cgcagaagca aaacgctgaa gtttgggctt gagatttgta ttcatagttt aagtgtatca
     2881 aaagaggcaa atgaaagcaa ctttaggaat aagtagatgg gagattcagt agaaataaaa
     2941 ctaatactct tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tactcaagta
     3001 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtataa ttctccaatt
     3061 tatagaatga aattgctttt atatctaaat tgctatataa taatgataag gaggaaataa
     3121 gtatgttaaa agaagtacta accgttgcaa aagttgcgaa aaaatcttca ttatttttgg
     3181 gtggtgtcgc atttggtacc cttggtttga aaatcttagc aagtaaggaa gctaaaaaag
     3241 gttattctaa agctttggct aaggcttaca agttgaaaga cgagctagat gcatctgttt
     3301 ctgttgtgaa gcaacatgga gacgatgtct tgcaagatgc caaatatttg tacgagcaag
     3361 agaaaaaaga agagcaatta gatagcctta taggtgaata atatgtcttt taaagtgcta
     3421 catagaggat accaacatat ccgactatca tcttcttttt cacttacctt ggatattcaa
     3481 gactatcttc gttccttggc gagagatgaa aaggggattg agtctatcca gttttacatg
     3541 gatcaacagc actttactct acgcataaaa gaaggctttt ctgtattaga taatgcagaa
     3601 gcctttttaa aaagaattga taaagggaaa gtttctgagt tgatgactct tcccattcgt
     3661 agagaagaga gtgcttattc tattgtttca ggtgcagcgg ttaagcgtgt actttttcgt
     3721 agttttgtgc cgtatcctat tcgctatata tggacttgtt atcaggcttt gggttatatt
     3781 agagaagcct atcaaacact agcgcgtaag gaactaacga tggaagtctt ggactgttcg
     3841 gctattttat tgtctttgtt tatgaaccaa tccaagacag ctagcaatat catgtttatg
     3901 cttgatttgg ggaatcattt agatcagtgg tccttgaaaa aaactgcaac agatttagaa
     3961 cagagtcttc ttgcaaaaga gagcgatgta ttcctagtac agggcgatac ggttgttagt
     4021 atcaagagtt ccgatgttca aataggagat gtcttgatct tatctcaagg aaatgaaatt
     4081 ctgtttgatg gacaagtagt ttcaggttta ggtatggtca acgaaagttc cttgacagga
     4141 gagagttttc cagttgaaaa aagagagtct gatttggttt gtgcaaatac agtattagaa
     4201 actggagagt tacgcattcg tgtaacagat aatcagatga acagccgtat tttacagctg
     4261 attaagttga tgaagaaatc tgaagaaaac aagaaaacga aacaacgcta tttcatcaag
     4321 atggcggata aagtcgtcaa atataatttc ttgggggctg ggctgactta cctattgaca
     4381 ggttcttttt ctaaggctat ttctttccta ttggtcgatt tctcctgcgc tttgaaaatc
     4441 tctactcctg tagcttattt gacagttatc aaggaagggt tgaaccgtga aatggtgatt
     4501 aaggatggag atgttctgga gaaatatctg gaagttgata ctttcttgtt tgataagaca
     4561 ggaacaatca caactagtta tcctatagtt gaaaaggtgt taccttttgg agactatagt
     4621 gaggaagata ttctcagaat cagtgcctgt cttgaggaac acatttatca tcctattgct
     4681 aatgccatcg tcaagcaagc tgagatagag ggaattgaac atgaggaaat gcatgggaaa
     4741 ctccaatata tcgcaagcaa ggggatcaaa tctcatatag atggccaacc agttcttatt
     4801 gggaattatg tcttgatgca ggatgagcag attcatatta gttcagaaca aaatgcttta
     4861 attgaagagt acaagagtca ctacaatctc ttattcttgg cttatcaaaa tgaattgatt
     4921 ggaatgttct gcattcatac tcctttgaga aaagaagcaa aaacagcctt ggataaactt
     4981 aaggcacagg ggaaaaaatt gattctggca acaggagaca ccttgattag gacagaggaa
     5041 ttagtcaaag atttgccctt tgatcaggtc tatacagact tgaaacctga tgggaaattt
     5101 gagttagtag agaaactgca gaaagcaggt cacactattt tgatggttgg agatggattg
     5161 aatgactcag cggctttaac tctatcagat atcggtgtgg tgatgaatga gagtgcagat
     5221 atttctaagc agatgagtga tatcttattg ttagataatc gtttggattt cttccaagag
     5281 ttggattcgt tatcatcatc tttgcaaaca ctcatcaaga aaaatattca agataccgtt
     5341 gtcgtaaata gtagtttgat tggctttggc ttatttaatt ggctcagtcc ttcaaatctc
     5401 tctatcttac ataatctaac aaccttgcgc attgtcctgc gtagcctgtc tattaaaaat
     5461 agatagatga gagttattaa cagcaaaaag gagttacctt tttcgaggtt aactcctttg
     5521 tttatagata aatgttgaat agtttagtaa gtcaatacaa agtatttctt cttcccacga
     5581 cggataacag tcagttcatt ctctaactta tcagcgtcac tcaagacata gtcaaggtct
     5641 tggatgcggt cgccgtttac gtagatggct ccgttttgga cgtcttcacg ggcttggcgt
     5701 tttgagttaa ctataccaga tgagacgagc agttccacga tattgttgtg ttcgtctgcc
     5761 tgtacttggt agtttggcac accacgaagt ccttgtttga gctctttgac agaaaggttt
     5821 ttgatgtttc ctgcaaagag ttgctcagtg atgttaagtg cttctttgta ggcttcttct
     5881 ccgtgaacaa gtgtaacaac ttcacgagcc aagacttttt gagccaagcg ttcgtgtggc
     5941 gctgcttcaa attgtttacg aatatcttca atctcatcaa gtgacaagaa agtaaagatt
     6001 ttcaagaagc gaacagcgtc agcgtccatc acgttcatcc agaattggta catttcgtat
     6061 ggagaagtct tttcgggatt gagccagacg gcatttcctt ctgatttacc aaatttctta
     6121 ccagtcgcat ctgtgattag tggaacagtg ataacgtgac cagtcttgtc cgccttacga
     6181 cgaagcaatt cagtaccagc tgtcatattt ccccactggt cagaaccacc gatttgaaga
     6241 gtgacattat ggtcttggtt aaggacgaag aagtcgtacc cttgcatgat ttggtaagcg
     6301 aactcagtgt aagaaattcc tgtttcgatc cgttttttaa cagattcctt actcatcatg
     6361 tagttgaccg tgaagtattt tccaatatca cggaggaagt caatgaagct gatgctgcca
     6421 aaccagtcgt agttgttgac catgacagcc ttgttttcgc cattttcaaa gtcaagaaaa
     6481 cgagaaagtt gtccttggat agacttgacc cagccatcta ctgtgtcttt tgtttggaga
     6541 ctacgttcag catctttgaa ggacggatct ccgatgagac ctgtagcacc gccaacgagc
     6601 gcataaggtt tgtgacctgc tagttgcaag cgacgacttg tcaagattgc gacaaggtgg
     6661 cctaggtgaa ggctgtcagc agttggatcg tagccagtat aataagaaac ttgaccttct
     6721 tctagggctt tacgcaaagc ttcttcatca gtcgtttgaa atatcaaacc acgctctttt
     6781 agctcatcaa aaatgtgcat gtgtcttttc tcctttttaa aattattgtt tctacctatt
     6841 gtatcacaaa cttgggcaat agcctagcag aatagggaag aaagtggcta ttttattgaa
     6901 aatttccctt ttgtggtata atagataaag tgaggaaatc catgcaaaat caattaaatg
     6961 aattaaaacg aaaaatgctg gaatttttcc agcaaaaaca aaaaaataaa aaatcagcta
     7021 gacctggcaa gaaaggttca agtaccaaaa aatctaaaac cttagataag tcagccattt
     7081 tcccagctat tttactgagt ataaaagcct tatttaactt actctttgta ctcggttttc
     7141 taggaggaat gttgggagtt gggattgctt tggggtacgg agtggcctta tttgacaagg
     7201 ttcgggtgcc tcagacagaa gaattggtga atcaggtcaa ggacatctct tctatttcag
     7261 agattaccta ttcggacggg acggtgattg cttccataga gagtgatttg ttgcgcactt
     7321 ctatctcatc tgagcaaatt tcggaaaatc tgaagaaggc tatcattgcg acagaagatg
     7381 aacactttaa agaacataag ggtgtagtac ccaaggcggt gattcgtgcg accttgggga
     7441 aatttgtagg tttgggttcc tctagtgggg ggtcaacctt gacccagcaa ctaattaaac
     7501 agcaggtggt tggggatgcg ccgaccttgg ctcgtaaggc ggcagagatt gtggatgctc
     7561 ttgccttgga acgcgccatg aataaagatg agattttaac gacctatctc aatgtggctc
     7621 cctttggccg aaataatagg ggacagaata ttgcaggggc tcggcaagca gctgagggaa
     7681 ttttcggtgt agatgccagt cagttgactg ttcctcaagc agcattttta gcaggacttc
     7741 cacagagtcc cattacttac tctccttatg aaaatactgg ggagttgaag agtgatgaag
     7801 atctagaaat tggcttaaga cgggctaagg cagttcttta cagtatgtat cgtacaggtg
     7861 cattaagcaa agacgagtat tctcagtaca aggattatga ccttaaacag gactttttac
     7921 catcgggcac ggttacagga atttcacgag actatttata ctttacaact ttggcagaag
     7981 ctcaagaacg tatgtatgac tatctagctc agagagacaa tgtctccgct aaggagttga
     8041 aaaatgaggc aactcagaag ttttatcgag atttggcagc caaggaaatt gaaaatggtg
     8101 gttataagat tactactacc atagatcaga aaattcattc tgccatgcaa agtgcggttg
     8161 ctgattatgg ctatctttta gacgatggaa caggtcgtgt agaagtaggg aatgtcttga
     8221 tggacaacca aacaggtgct attctaggct ttgtaggtgg tcgtaattat caagaaaatc
     8281 aaaataatca tgcctttgat accaaacgtt cgccagcttc tactaccaag cccttgctgg
     8341 cctacggtat tgctattgac cagggcttga tgggaagtga aacgattcta tctaactatc
     8401 caacaaactt tgctaatggc aatccgatta tgtatgctaa tagcaaggga acaggaatga
     8461 tgaccttggg agaagctctg aactattcat ggaatatccc tgcttactgg acctatcgta
     8521 tgctccgtga aaagggtgtt gatgtcaagg gttatatgga aaagatgggt tacgagattc
     8581 ctgagtacgg tattgagagc ttgccaatgg gtggtggtat tgaagtcaca gttgcccagc
     8641 ataccaatgg ctatcagacc ttagctaata atggagttta tcatcagaag catgtgattt
     8701 caaagattga agcagcagat ggtagagtgg tgtatgagta tcaggataaa ccggttcaag
     8761 tctattcaaa agctactgcg acgattatgc agggattgct acgagaagtt ctatcctctc
     8821 gtgtgacaac aaccttcaag tctaacctga cttctttaaa tcctactctg gctaatgcag
     8881 attggattgg gaagactggt acaaccaacc aagacgaaaa tatgtggctc atgctttcga
     8941 cacctagatt aaccctaggt ggctggattg ggcatgatga taatcattca ttgtcactta
     9001 gagcaggtta ttctaataac tctaattaca tggctcatct ggtaaatgcg attcagcaag
     9061 cttccccaag catttggggg aacgagcgct ttgctttaga tcctagtgta gtgaaatcgg
     9121 aagtcttgaa atcaacaggt caaaaaccag ggaaggtttc tgttgaagga aaagaagtag
     9181 aggtcacagg ttcgactgtt accagctatt gggctaataa gtcaggagcg ccagcgacaa
     9241 gttatcgctt tgctattggc ggaagtgatg cggattatca gaatgcttgg tctagtattg
     9301 tggggagtct accaactcca tccagctcca gcagttcaag tagtagttct agcgatagca
     9361 gtaactcaag tactacacga ccttcttctt caagggcgag acgataattc tctaaatgaa
     9421 gtggccaatc aatggattgc cacttttttc ctttcgtgtt acaataaggg tatgaatcag
     9481 tatcagaaaa agattgttaa cggaaaaatt tattcgctcc tatccggctt aatatgggga
     9541 atctgtggaa ttttaggaga gtacttcttt actcattatc aggtgtcttc gggctggatt
     9601 acctctatgc gtttgacact ggcagggagt cttgtactca tttggtctgc aatacaatta
     9661 aaatcgcaag tgctagatat ttggcgagac aagaaaaatt acctgccctt tttagcctat
     9721 gctattttgg ggattttttc agtccagtat tttttctatc tctgtgtaga atactcaaat
     9781 gctacgacag caactatttt acagtttatt agccctgtct ttatcctctt ttacaatcgc
     9841 ttggtttatc aaaaacgagc gtcaaaaagc gctgttttct atgttttggt tgccatgctg
     9901 ggtgtttgct tgatggcgac aaagggagat ctctctcagt tatccatgac gccgctagct
     9961 cttataacag gtttgctgag tgccatgggt gttatgttta atgttatctt gccccaacct
    10021 tttgctaagc gttatggttt tgttcctacg gttgggtggg ggatgatttt ggcaggtttg
    10081 tttagcaatg tcctctcgcc ggtttatcag ctttccttta ctcttgatat ttggagtatc
    10141 ttgatttgcc tcattatcgc tttctttgga acggcttttg cttttttcat ttccatgaag
    10201 gctgtgtcct tggtttctcc tttggtggtt gccgttatca gtgccagtga acctctctct
    10261 tctgctctct tgagtgtttt gttcttagga ttagtagtgg attggtccct ccttctagct
    10321 atagccttga ttattttacc catgatattt ttgtctatag aagaagcgaa agaaagtaga
    10381 taaaaagtct tttcttaatt ctaaaagtgt gctatactag aatagtcaat aaaacacggg
    10441 gagatagttg tgaagagtgt ttttaggttg tatcggtagg ggcttgcttt taccgacagc
    10501 acgttttatc tcacatccct aaaaaatcat acctaaaaac agacaaaaag gtttcaaatt
    10561 tgaggccttt tttggtagaa taggtatcat tataacgaac caggaggcac ctatgactgc
    10621 tacaaaaatg aacgctcaag aaattatcca atttatcgcc aatgctgaaa agaaaaccag
    10681 tgtcaaagta acctttgagg ggcaactcgc aactgctgtg cctagctctg ttgtcaaact
    10741 agggaatgtc ctattcgggg attggaagga cgtggctccg cttcttgaag gtttagtaga
    10801 aaatcaagat tatgttgtcg agcaagatgc tcgtaattct gcagttcctt tgctagataa
    10861 gcgtgctatc aacgctcgta tcgagccagg tgctattatc cgtgaccagg tggaaattgg
    10921 tgacaatgtt gttatcatga tgggagctgt tatcaatatc ggtgctgaaa tcggtgctgg
    10981 aaccatgatt gacatgggtg ccatccttgg tggccgtgcc atcgttggaa aaaatagcca
    11041 cgttggtgca ggtgcagttt tggcaggtgt gattgagcca gctagtgctg aaccagtccg
    11101 tgtcggagac aatgttctca tcggggctaa tgcagtggtt atcgaaggag tccaaatcgg
    11161 tagtggttca gttgtcgcag caggagctat tgttacccaa gatgtcccag aaaacgtggt
    11221 agtagcaggt gttccagctc gtattatcaa agaaattgat gcccaaactc aacaaaaaac
    11281 agcgctagag gatgcgcttc gtaccttgta attgtaaaag taaaaaagag gcggaaccct
    11341 ttttccagcc tctttctgct atatcggagg acagatagat gttagatttg attcagacta
    11401 gacgagattt acaccagatt ccagagattg gcttggagga gttcaagact caggcttatt
    11461 tgctggatgt gattgagaaa ttgactacgg gcaaggattt tgttcaaatt cgtacttggc
    11521 ggacagggat tttggtctac ctgcagggaa gtcagccgga gcgaaccatt ggttggcgga
    11581 cagatattga tggcctgcct atcgtcgaac aaacaggtct accttttgct tctcaacacc
    11641 aaggtcgcat gcatgcctgt ggccatgatt ttcatatgac cattgcctta ggctgtcttg
    11701 agcgcgccct tgaggagcaa cccaagaata atctgctctt tctatttcag cctgctgaag
    11761 aaaatgaagc tggtggtatg ctcatgtatg aggctggtgc ttttggagat tggttgccag
    11821 atcaatttta tggcctccat gtccgtccag atttgaaagt cggccagatt gcgactaata
    11881 ctcagacact ctttgcagga acttgcgagg tgaagatccg tttcaaaggc aaaggtggcc
    11941 acgcagcttt tcctcatgaa gccaatgacg ccttggtggc ggctagttac tttgtgaccc
    12001 aagtgcagtc agttgttagc cgcaatgtca acccaatcga gggagcagtg gtgaccttcg
    12061 gcgtttttca agctggaacc accaacaatg tcatcacaga cacagctttt ttacatggaa
    12121 ctattcgtgc cttgacacag gacatgagtc tcttggtgca aaagagggta aaaacagttg
    12181 cagaaggggt tgcagctgcc tttgatatgg aagtcgaagt ggaactcaaa caaggtggat
    12241 acctacctgt tgagaacaat ccagccttgg cgcgtgaact gatggacttc tttgacgaaa
    12301 aagacggaat cgagttgatt gatatcgaac ctgctatgac aggcgaggac tttggttatc
    12361 tcctttcaaa ggttgatggt gtgatgtttt ggctaggtat cgatagtccc tacgctcttc
    12421 atcaccctca aatgagtcct aaggaagaag tcttagctat tggggtggct gcggtgtcta
    12481 gtttcctgaa aaagaaggca gcagaataga ggggttgtct atgaaatcgg aattacgcaa
    12541 gcaagtcttg catgaaatga aggctttatc tcaagaacaa aaacaggcta tagaccaagc
    12601 tttaaccgag cggcttttac aacacctctt ctatcaagaa gccaaggtca tcgcaaccta
    12661 cctctctttt tctcatgagt ttcaaacgcg ggaactgatt gagcaggcgc tgaaggacgg
    12721 caagaaggtt ttaataccca aaacttatcc caaggggcgc atggagtttg tggtctatga
    12781 tccgcaacag ttggtaaaaa cttcctttgg attactggag ccacagggag atttggaagt
    12841 tgtgaatgtt tcaaagattg atttgattca tgttcctggt ctggctttta cgacgaaagg
    12901 atatcggata ggatacggtg gaggctatta tgaccgctat ctggaacatt tttctggtcg
    12961 tgctttgagt acgatatatc cttgtcaaat tcaggacttt atacctgaaa accatgatat
    13021 tcctgttcag gaggtattaa ttgatgaagg aaatctttga tagacgttac cctgtgacga
    13081 gtttcttcct cttagtgacg gccttggtat ttttactaat gttggtcact acaggcggaa
    13141 actttgacag ggcagataca ttatttcgat ttggagccat gtatgggcca gctattcgcc
    13201 tctttcccga gcaggtttgg cgtctcttgt ctgccatttt tgttcatatt gggtgggaac
    13261 atttcattgt taatatgctt tcactttatt atcttggaag gcaggtagag gagattttcg
    13321 gttctaagca gtttttcttt ctctatcttt tatcaggaat gatgggcaat ctctttgttt
    13381 ttgtatttag tcctaaatcc ttagcagcag gcgcctctac ctctctttat gggctatttg
    13441 ccgcgattat tgttcttcgc tatgcaactc gcaatcctta tatccaacag ctagggcaat
    13501 cctatctgac actttttgtg gttaacatta ttggaagtgt tctgattcca ggaatcagcc
    13561 tagcaggcca tatcggtggt gcagttggtg gcgcatttct agcagttatc tttccagtta
    13621 gaggagaaaa acggatgtat aacaccagcc agagattagg agcggtagtc ttgttcgtag
    13681 gactcgccat tttgcttttc tacaagggaa tgggaatgtg acgaagttga aagaaaattt
    13741 gtgtaatgaa aaaagataag gcattttttg aaccttatct ttttatttta ttttattcct
    13801 tctcagtcaa ttcttttcca agttggatga ggtaattctt caaatcatct ttgacttgtg
    13861 gatgtttgag ggcgtagtca atagatgttt tcataaagcc aaacttgtct ccgacatcgt
    13921 aacgagcccc attgaactca cgagcaaata cacgttgtgt tttattgagg gtgtcgattg
    13981 catctgtcag ctgaatttca tttccagcac caggagcttg gttttcgagg atttggaaaa
    14041 tttcaggcgt gaggaggtag cgtccgataa tagcaaggtc gctaggagcg tcctctggag
    14101 ctggtttttc aacaaaggtt tcaacactgt aaagaccatc ttttccttcg ccttgcggag
    14161 caataacccc gtaagcagat acttcgtcat gagggactgg catgacagcg atagtagacg
    14221 cgtgggtacg ctcgtagtca tccatgagtt gtttggtaag tggaacagcc ttttcatctg
    14281 tgatatccat caagtcatca ccaagcataa cgacaaaggg ttcatttcct acaaaagctt
    14341 tggcttgtaa gacagcgtct ccaagaccac gagggtgagt ttggcggatg aaatgaaggc
    14401 gcatgccagt tgcttcatct accaatttta acaaatccgt tttgcttttt tctttaagat
    14461 tgtattcaag ctcaaagttt gaatcaaagt ggtcctcaat agaacgtttt gatttaccag
    14521 tgacaactag aatatcttca atacctgatt tgagagcctc ttccacgata aactggatag
    14581 ttggtttgtc tacgattggc aacatttctt tggcaagggc cttggttgct ggtaaaaatc
    14641 gagttcctag tccagcagca gggatgactg cctttctaac ttttgatgtc ataagaatcc
    14701 tttctataga gggttaagac cactcatttt ctgctttaaa ttcattgttc atgatgtcat
    14761 aaatggtatc tttgatattg gttccgtgat aaataacttg gtaaatggcc tgtgtaatgg
    14821 gcatatagac tccaagttct tgggctagtt cataggctgc tcgagtcgtt gaaattcctt
    14881 cgattaccat gcccatatta gcttctatat cagctaggga ttctcctcgt ccgagagcat
    14941 ctccagctct ccagttacga gagtggatgg aagttcccgt tacgatcaaa tctcccacac
    15001 cagataagcc gctataggtc aatggactgg ccccgagagc cacccctagg cgggtgattt
    15061 ctgctaaacc tcgagcgatg atggctgcct tagcattatc accaaatcca agaccatgta
    15121 aagctccagc accgacagca ataatatttt taagagcacc agcagtttca accccgataa
    15181 catccgtatt ggtataaagt cggaagtagt gattactaaa tagctcctga acgtattgag
    15241 ctgtttgtaa atctttagaa gcagcagtta ttaaagttag gtcacgcaca atggtctctt
    15301 ctgcatgact aggtcctgaa acaacgacga tatcactacg gagatgttca ggaatttctt
    15361 cttcaagaat gattgataat cgtttatggc tatcaggttc taatcccttt gatgcgtgca
    15421 tgatgataac cttatggtcc aaggtttgtg caacttgctg ggcaacaagt cgtgtcactt
    15481 ttgttgggac aacaaacaaa atcgcatcca catctttcaa tgtttctgct aagtcggtgt
    15541 aggcaatgat attttcgtct agaacgacat ctttaaagta gtgcttatta gtatggtgtg
    15601 tattaatttc attgatttgc tcgggaagat ttccccaaat acgtacctcg tgtccattgt
    15661 catttaagac ttgtgaaagg gcggttcccc aagaaccagg ccccaagacg gcgacagttt
    15721 gttttttcat gatttcctcc ttgatagggt tctttctctt attctatcat attctagggg
    15781 attttgcact tgcattactg gggtatggtg gaaaaataca caaaaaccga gacgaaggga
    15841 taaactctta gtctttagtt aaaatgagaa gaaatctgat agatagtttg attcagtata
    15901 tgcttataat agaggtaaac aactcaggag gttcttgtag gttgcgagag tttggcgaaa
    15961 aaattaaaag attacgtttg gctaaaaaaa tcagtcgttc agaattttgt ggtgatgagt
    16021 ctgaattaag tatccgtcaa ttaattagaa ttgaaaatgg agaatccaga ccaacactaa
    16081 caaagttaaa atatatcgct gaacgtttgg gggttgaaga ttacaagttg atgccaagtt
    16141 atatagagtt ggataaggaa tacctagaat tgaagtattt cttgatgagg actcctacat
    16201 acgaagatga aactatcgcc caaaagaaag agagtgtttt tgctaagatt tttgaagagt
    16261 attatgatag gctacctgag gaagaaagat ttatcatccc aaattattca tatctggcac
    16321 taacgaacta cacagttcaa aaattaccag aaaagctagt tgaaatactg tccttctggt
    16381 gattttgctt aatttctgat gttcgagaca tgattgagga gatttgtctt gattttttcc
    16441 taaaaaaggg cagactcctc ccttggtttg atgctcgatt tttaaaataa ggtgacttat
    16501 tctgttaggc atgttttccc ctgccctcta gctctatatg gaacagggag tatcagattt
    16561 atctggcaga ttgtatcaaa taaggcttga aactgttttg aataggcgta tagacttgag
    16621 aatttgagaa tatgtcgtct agcagtagag acatattttc cggcaatatg aaggaagaga
    16681 cgtcgaaaac gcttgatagt caaggacttt acttcatcac cagctagctg ctttaaaaag
    16741 aggtagagat tataagccag acagcccatc atcatacgta cttcattctt aatcatggtc
    16801 gaactatctg tcttatcccc aaagaatcct gctttccttt ctttgataaa gttttcagca
    16861 ttccctcgtt cacgatagaa ttgaaacagc tcctcactat ctacactgct taaattgttc
    16921 aaatgaactt ttttacaacc ataaaaaaga gcacttaatc gtactcttct cttttaagga
    16981 ttagttgaat taattcaaat ccactagttc tcctgtttct aggtagacta ggcgttcaca
    17041 aatgttagca atgtaatcag cgaagcgttc cagatgccct atgatataaa ggtattgagt
    17101 tccattggga attgaggttt cttggtcttt cataagtcca atgatttcct ttgataaggc
    17161 ataataatat tggtcaatct gttcatcttt ttgagcaata ctaatagctt ttgaggcttg
    17221 gtgcaaagga aaggcaacca ataaatcggc tagcatgctg agggataatt tacccatttg
    17281 gtgtaactgt tcttcgtcag gggctagttg attttctttt agttgcaaaa cagctttggc
    17341 aatgcctgcc atatggtctc ccatacgttc aaggtctgaa caagaagaca tgatgctaat
    17401 cacaaatcga aggtcagaca cttgtggctg ctgcaaggct aacaaacggg cacaggtcaa
    17461 ttcgatagcg ctttgacctt ggttgatagc atgatcctta ttgataatta gctctgccat
    17521 ctccttgtct ttggaggcta aggccagtaa ggtttttgaa gctgtttcaa ggacaagttg
    17581 ccctagtcct aaaaaggatt gttctaattc atgcaattct aagtcaaatt gatttctcat
    17641 aggtttttcc ttccttatcc gaaccgtcct gaaatatagt cttctgtgcg ctgatctttt
    17701 ggattggtaa acacgtcaac ggtatctcca aattcgcaaa tttctcctgt taagaaaaaa
    17761 gcagttttat ctgaaatacg tgaagcttgt tgcatgttat gggtaacaat gataatcgta
    17821 taatcctttt ttagttgctg aatgaggtct tcaattttta aagtggagat agggtctaag
    17881 gctgaagtcg gctcatccat taacagaata tcaggttcta ctgctaaagc tcgcgcaatg
    17941 caaaggcgtt gctgctgacc gccagataag gacatggcac tctttttaag atcatctttg
    18001 acttcttccc aaatggctgc cccttttaaa gatttctcca ctaaggcatc taattgtttt
    18061 ttgtctcgaa taccatgtgt ccttgggcca taagccacgt tatcatagat agacatggca
    18121 aagggattag gctgttgaaa aaccatccct acacgcttac gtagctgatt aaggttgaat
    18181 ttgctactat aaatatcttg ctcatctaag aggacttggc cttcaatatg gcaagaagga
    18241 accaaatcgt tcatccggtt aagggttttt agaaaagttg atttgccaca accagatggg
    18301 cctatcaagg cagtaatctg tctttctggt aattgaatcg aaatattttt taaggcttga
    18361 aaatccccgt aaaataagtc taggtgtctg actgaaaatg ttcccatact aggaagctcc
    18421 tttcacaagt tttcgagata ataagcttga tagagtattt atcattaaaa cagtaataat
    18481 caaaatcaca ccggtagcat aggcttcatt gacatgtagc ccctcacttg acagcatata
    18541 catatgcagg gctagagaac gacctgaaga catgagacta cttggcgtat tggtagaggt
    18601 acctaatgta tacatgaggg cagctgtctc accaacgata cggccaatag ctagtatcac
    18661 tccagctaaa atacctggca tggcaactgg tagaacaatt ctaaaaacag tccgtaactt
    18721 acctgcccca agtccataac ttgcttgacg catgctatca ctaacagata aaagggcttc
    18781 ttctgttgag cgaataatga ctggcaacac catgatgact gaggttaaga ttcctgataa
    18841 cagagagtat tgaaaaccta agaagactac aaagaagagc atgccaaaca gaccaaaaac
    18901 aatggaagga atcccagaca aggtatctga ggccaatcgc atgattttaa cacaaaggga
    18961 atcttttttt gtatattcca caagataaaa accagcaaaa atccctatgg gcaaggctaa
    19021 aagaagagca ccaaagacca gaataacggt ggaaataatc gctggcataa gggaaatgtt
    19081 ctcagaagta taagtccaag aaaagaggga tagacttaga tgaggtaagc ctttgatgag
    19141 gataaaacca atgattaaaa agagagagcc aaaggttaaa gctgaaaaac aataaacgag
    19201 aagttttagc aggtatttac tcataagatg attttccttt caagtaggca aagtaggcat
    19261 taatcaagag aataaggaaa aagagaactg ctgaggttgc aataagggct tccctatgct
    19321 gacctgatgc gtaagccatt tccagaacaa tattggttgt taaggttctg gttcctgaaa
    19381 agagtccact tggaataatc ggctggttgc ctgccaccaa aataactgcc atggtttcac
    19441 ctactgcgcg accgattcct aaaataactg ctgataaaat accagatctc gcagctggca
    19501 agatgacact aaaaatactc cgttcatgac tagctcctag agccaagcta ccagaataat
    19561 acgttttggg aactgttcgg atagcagatt ctgacaaact gataatggtt ggcaaaatca
    19621 ttattcctaa tagtaacgaa gcggttagga cactcatgcc atttcctaaa aagcttctaa
    19681 tccaaggcac caataattgt aggccgaaaa aaccataaac aatagatgga atggccgcca
    19741 tcaagttgat agctgatttt aagaagccat agacgggctt tggacaataa taaaccataa
    19801 acaccgatgt caagatgcct gttggcaccc caatcacaat cgctcctaag gtaattaata
    19861 aggaaccaac gatcattggt aaaataccat agcttgccgg aatgttcgtt ggcgaccaat
    19921 cactgcctaa taaaaaacgg gcaaagccgt agttagccat gaaaggtaag ccattactaa
    19981 aaataaagaa acagattagc aaaatagcta caacagctac tgttgcactc atgaaaaaaa
    20041 ttgccctaaa aactgcttct ttgaaggctt gttttatcac atcttgtcct ttctagtgaa
    20101 gaaagtaagg gagatacgac acctccctac ttgccttctt tatcttattg tacgatgaaa
    20161 cgtctgcatc tctttagaga tttatggagc aaacatttta tttaatcttg tcccaggtgg
    20221 ttaatttgcc actaaaaacg tctgcaagtt cagccatact gacttggctt gccttattgt
    20281 cattattgac cacaacagca ataccgtcta aagcaatagc atcatgggtg agactcttac
    20341 cttcttcagg agttaattcc ctagaaacca taccaatatc agcggttttc tccttaacag
    20401 cggtaatacc tgctgaagac ccattagagg taatatcaat cgtaacttct ggattttctt
    20461 ttttataagc ttctgctaat ttttccatta aagaagatac tgaagtggaa cctacaacag
    20521 acaacttgcc tgataagtgt tggcttgtat attctgtggt ttcggtttta gcttcaataa
    20581 atttattatc tgtgaccact tgttgacctt gtttggagtg gataaagctg ataaaatctt
    20641 gacctagctt ggaaagatta gaagaccaaa caatgttgaa gggacgttga agagggtatt
    20701 caccatctaa aactgtgtct cgactagcct tgacaccatc aatctctaaa gccttgacag
    20761 atttcgttaa agatcccaag gagatgtagc cgatagcatt agcattccct tgaactgctg
    20821 agagaacacc ttctgtacta ttttgaatca cagctgtttt ggcagtgttg tcaatttttt
    20881 tatcaccgtc ttttttgaga atccctgtga tttctgtgaa ggcaccccgt gttccagagc
    20941 cattttctcg tgaaatcacc tcaatcgttc ctgaagctga ctgtttggaa gcagctgact
    21001 gattgccaca ggcaacaagc ccaaatcctg ataagccaat ggctgcaaga gtaagcattt
    21061 ttttgaattt cataataatc acctttatct ctatgtattt ttcttgtgta ggcttactac
    21121 atttatagtc taacaagtct ttgtaaaggt ttatccctga ttcatgtaaa gattgtgtaa
    21181 agaatcaaaa aaagccactt ttgaaaaatg gctgctccta aaaatagctt taaaaattat
    21241 tagtcctgtg cgaaagattg gttaggaaga aaaatcgtga agcaactgcc tctgccaagc
    21301 tgactcgtca ccgtgacttg gccacctaat aattgactga gttctttgac aatggcaagg
    21361 ccaagaccag tgccaccagt ttgtctgctt cgacctttat taactcggta aaaacgttca
    21421 aaaatacgat cctgctctaa ttgactaata ccaatccctg tatctgatac agaaatctta
    21481 atgccttcgt tcaccttttg ggtcttgacc tcaatttccc ccccttgttc agtgtaacgg
    21541 atggcattgg ataaaagatt gagtaagatt tgggaaagta attgactatc tgatacgagg
    21601 gtgacatcat ctggcacctg cacctttagc tgtaaatcct tcttcttgag ctgaggttgc
    21661 aagctttgag tcaaatcctg tacaaattct gccaaagaaa gggtcgtcca ttgtataggc
    21721 atttgttgag ccttagataa ggtaagaaga tgctcaacaa tatgctcaag acgcaaactt
    21781 tctttgtaaa taatgtctag aaagtcatcc ttgagcgctt cttcttcagc tgacatcccc
    21841 ttaatggttt cagcaaagcc cttaatcgaa gtaactggtg tcctcaattc atgggaggca
    21901 tttgagacaa aggctaaatt taacttttca taagttctaa tcgttgttaa atcatatagc
    21961 aagacgagca cagcttccac agattgggtg gggctaaaaa cgggaactgc tgtcacttct
    22021 aaaatcaagt caccctcatg aaacccactt acttcttgtt ttaaccttgt tttttgatca
    22081 aaggcttggt gaactaaatt ccgaatatcc atccgtttga ggtcatcaag tgaacttatg
    22141 tcgccgtcca catcgggaaa ataatgaggc agagagcgac tggataataa catctgacct
    22201 tgagcggaaa ctaaaaacgt ccccatggtt aggtgtgaca gaagaacctc cattgtttcg
    22261 gctagatcct tgtattgctg atcctgttgg gagactttgg tttttaggcc agacacatac
    22321 tgagccaaag actttaagtc ttcttgccct ttttctaaaa agtattcact actggtcaag
    22381 agaggttggt gcaaggtctc aaaagcaact tcccatttcc aaaggcaaaa gagccagtag
    22441 ccacctagtc ccaaagaaag ggctagaaga aagagaccga tgcctttact gatccaagtt
    22501 aatgccatcc ctgcaatcag aatgaggcta acacttagat tgactagcca aaattgaagg
    22561 tagcgtttca tctataactc cttgaactta taaccataac cccgaatggt tcgaataaat
    22621 tgaggggctt taggattgtc ttcaattttt tccctcaact taccaatatg aacgtccacc
    22681 aaacgtgttt cctgcccaaa gtcatacccc cagatacgtt ccaaaagacg ctctctagtc
    22741 agtgtcatgt tgggatgttt cataagatag agcaagagtt caaattcttt tggggtcaaa
    22801 ctcagtaact tattcgcctt gtagacttca tgacgctcag ggtatacttt caaggtccca
    22861 aatagccaag aatcgtcagc gatattatct gaatcatctc cttcttgttc tcctttagtt
    22921 cgcctgagga cagccttgac acgcgccagc aattctctag ggctaaaagg cttggtcagg
    22981 tagtcatcag cccctaattc caaggccaaa accttatcaa attcatcact tttcgcagaa
    23041 accatcataa tcggagtttt gacgcctttg gctctcagcc gcttacaaac ttccatgcca
    23101 tctaattgtg gtaacatgat atcaagcaag ataaaatcaa agggttctgt ttctgccaaa
    23161 gctaaggcct tccgtccatt tgttaccaat tgagtagaaa agccttcctt acttaaatgg
    23221 tagtcaagca atttcagaat gtgttcttca tcatccacta ataagacttg ttttgtcatc
    23281 tattatctcc tattggtaac attataacac aattatcaga aatcctaaca ttgctaaatc
    23341 agattaaatt tgcttatcaa gactagtatc tggtcaaacg ctcaatcatc tccttgtgct
    23401 ctggataggt cgccagtaga tctacccttt caaataattc aaaatcctca aattcaaaac
    23461 caggagcaac aagacaagaa accagagcat aatccttatc aactgttgat ccccaaatag
    23521 tgcccttagg aacacagtag tgaagttgtt gccctttgga tatgtccagg cctaaagtga
    23581 ctacttcgta gtgaccatct gctgtaatca tgtgaacagt aagtggggat cctgcatgaa
    23641 aataccagat tccatctgct gtcaatcggt gaaaatgtga aggattcgtt tcttctaata
    23701 agaaataaat actggtataa agcgcccttc ccttaccagc aaggtttatg gtgtctgaag
    23761 ctttttttgt ttgtctaaaa tagccacctt caacatgggg agttaactct agagttctta
    23821 tcaagtcttc tttatccgtc ggagccaatg ggttgaagta actcttgttc aaaggtggtg
    23881 ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat gtgacggaag
    23941 taaaaatcaa tcgccctaaa aaacaattag cgaattattc tggtaaaaag aaatgccacg
    24001 ctatgaaggc tcaggcgatt gtcacaagtc aagggagaat tgtttctttg gatatcgctg
    24061 tgaactattt gttatgatat gaagttgttc aaaatgagtc gcagaaatat cggacaagct
    24121 ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa
    24181 actccacgta aatccagcaa actcaagccg ctaacagttg aagataaagc ctgtaatcat
    24241 gcgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa aacgtttaaa
    24301 atgttttcaa caatctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt
    24361 gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctat tatttggtta
    24421 ggtgaattag tgaagcgttt aggcaagtgt ctctggttac gacgtcatgg cctctaaatc
    24481 gattatattt aggggtcatg actagtgaag cagttagcta gttcgcatat aagcggctag
    24541 cgtctaacaa ttaggaactt tagttccaat aactttaaga ttacgacgtt ttaggacata
    24601 aatcgatcat atttatgtcc taaaactagt gaagcaccta gccaaagtcc gaataggatt
    24661 tggcgttagt tacttagatt gctttgcaat caagtaactt tggcgattta catcttctct
    24721 ggcgcttcta ctccaagcaa gcgaagggct tctttgagaa cgactgcggt tgcgtagctg
    24781 agggctagac ggctgtcgcg ttctgggctt tcatccaaga tacgtgtatg tgcatagtat
    24841 ttgttaaagg attgagccag gctaattgca aatttagcaa tgatagaagg ttcaaagtta
    24901 tctgccgcac ggttgataat acgtgggaag tcttgaatga gtttaatgat ttcccagctt
    24961 tcagtatcat tcaagctata gttgccagct gtttctggtt tgaaatcggc tttgcgtaag
    25021 atagattgga tacgagcgta ggcatattga acgtaaggtc cagtttcacc ctcgaaggat
    25081 accatagcct ctaggtcgaa gtcgtatcca tttgtacggt cggttttgag gtcatagaat
    25141 ttaatggctc caaccccaac agcatgtgct acctgatctt tattttccaa ttcaggattt
    25201 ttagcctcga tttggacctt ggcacggcta acagcctctg caacagtagg ctctagcaag
    25261 atgacattcc ctttacgagt agagagtttc ttcccttctt ttgtaaccaa accaaaagga
    25321 acgtgagtaa tgtcgtcact ccagtcgtag cccatctctt gcaagacagc tttgagctgt
    25381 ttaaagtggg cagattgctc ttgaccaacg acgtagatag atttagcaaa ttggtattca
    25441 tttttacggt agagggctgc agccaagtca cgtgtgatat agagagttgc accatcagat
    25501 ttcttgatga gggctggatg ttcaattcca tatttctcaa gattcacaac ttgggcacct
    25561 tctgattcaa gaagtagtcc tttttcagaa agaatgtcta caactgcatc catcttatca
    25621 ttgtagaagg cttctccgtt atagctgtca aattcaacct tcaattcatt gtaaaggcgg
    25681 ttaaattcta ctaaactttc atcgcggaac cattgccaaa gagcgagagc ttcctcatct
    25741 ccattttcaa gtttacggaa ccattcgcgc gcttcttcat ccaagctagg gtcattttca
    25801 gcttcagcgt tgatgcggac atagagttta aggagttcat cgattggatg agcttttaca
    25861 gcttcttcgt cgccccattt tttgtaggca acaatcaaca tcccaaattg tttaccccag
    25921 tctcccaaat ggttgacctt gaccgtttga taaccgattt tttggaaaat atgtgacaag
    25981 ctatctccga taacagttga acgcaggtgg ccaatagaaa atggtttagc gatattcgga
    26041 ctagacatgt cgataacaac attttcttgt ttgccaatat tttggtcagc atagtgttct
    26101 ttttcagtgg taacagcttg caatacttga gcagaaatgg cagatttatc aaggaaaaag
    26161 ttaacgtaag gtcctgttgc gacaactttt tcaaaggctt ggctgttcat tttttcagcc
    26221 agttcagccg caatcatttg tggtgcttta cgttcgactt ttgcaagaga aaaagcaggg
    26281 aaagcaatgt ctcccatttc tgagttttta ggggtttcca gtaactttaa aatagcctct
    26341 tggtccaggc tatcaatgat gctagataat tcgctagcaa tcaattcttt tgtattcatt
    26401 aagagctcct ttttggactt ttctactatt ttatcacaat tttaaagaaa gaagaaaaaa
    26461 tttttgaaat ctcctgtttt tttggtataa tatggttata aatatagtta taaatatgca
    26521 cgcaagagga ttttatgaga aaaagagatc gtcatcagtt aataaaaaaa atgattactg
    26581 aggagaaatt aagtacacaa aaagaaattc aagatcggtt ggaggcgcac aatgtttgtg
    26641 tgacgcagac aaccttgtct cgtgatttgc gcgaaatcgg cttgaccaag gtcaagaaaa
    26701 atgatatggt gtattatgta ctagtaaatg agacagaaaa gattgatttg gtggaatttt
    26761 tgtctcatca tttagaaggt gttgcaagag cagagtttac cttggtgctt cataccaaat
    26821 tgggagaagc ctctgttttg gcaaatattg tagatgtaaa caaggatgaa tggattttag
    26881 gaacagttgc tggtgccaat accgtattgg ttatttgtcg agatcagcac gttgccaaac
    26941 tcatggaaga tcgtttgcta gatttgatga aagataagta aggtcttggg agttgctctc
    27001 aagacttatt tttgaaaagg agagacagaa aatggcgata gaaaagctat cacccggcat
    27061 gcaacagtat gtggatatta aaaagcaata tccagatgct tttttactct ttcggatggg
    27121 tgatttttat gaattatttt atgaggatgc ggtcaatgct gcgcagattc tggaaatttc
    27181 cttaacgagt cgcaacaaga atgccgacaa tccgatccct atggcgggtg ttccctatca
    27241 ttctgcccaa cagtatatcg atgtcttgat tgagcagggt tataaggtgg ctatcgcaga
    27301 gcagatggaa gatcctaaac aagcagttgg ggttgttaaa cgagaggttg ttcaggtcat
    27361 tacgccaggg acagtggtcg atagcagtaa gccggacagt cagaataatt ttttggtttc
    27421 catagaccgc gaaggcaatc aatttggcct agcttatatg gatttggtga cgggtgactt
    27481 ttatgtgaca ggtcttttgg atttcacgct ggtttgtggg gaaatccgta acctcaaggc
    27541 tcgagaagtg gtgttgggtt atgacttgtc tgaggaagaa gaacaaatcc tcagccgcca
    27601 gatgaatctg gtactctctt atgaaaaaga aagctttgaa gaccttcatt tattggattt
    27661 gcgattggca acggtggagc aaacggcatc tagtaagctg ctccagtatg ttcatcggac
    27721 tcagatgagg gaattgaacc acctcaaacc tgttatccgc tacgaaatta aggatttctt
    27781 gcagatggat tatgcgacca aggctagtct ggatttggtt gagaatgctc gctcaggtaa
    27841 gaaacaaggc agtcttttct ggcttttgga tgaaaccaaa acggctatgg ggatgcgtct
    27901 cttgcgttct tggattcatc gccccttgat tgataaggaa cgaatcgtcc aacgtcaaga
    27961 agtagtgcag gtctttctcg accatttctt tgagcgtagt gacttgacag acagtctcaa
    28021 gggtgtttat gacattgagc gcttggctag tcgtgtttct tttggcaaaa ccaatccaaa
    28081 ggatctcttg cagttggcga ctaccttgtc tagtgtgcca cggattcgtg cgattttaga
    28141 agggatggag caacctactc tagcctatct catcgcacaa ctggatgcaa tccctgagtt
    28201 ggagagtttg attagcgcag cgattgctcc tgaagctcct catgtgatta cagatggggg
    28261 aattatccgg actggatttg atgagacttt agacaagtat cgttgcgttc tcagagaagg
    28321 gactagctgg attgctgaga ttgaggctaa ggagcgagaa aactctggta tcagcacgct
    28381 caagattgac tacaataaaa aggatggcta ctattttcat gtgaccaatt cgcaactagg
    28441 aaatgtgcca gcccactttt ttcgcaaggc gacgctgaaa aactcagaac gctttggaac
    28501 cgaagaatta gcccgtatcg agggagatat gcttgaggcg cgtgagaagt cagccaacct
    28561 cgaatacgaa atatttatgc gcattcgtga agaggtcggc aagtacatcc agcgtttaca
    28621 aactctagcc caaggaattg cgacggttga tgtcttacag agtctggcgg ttgtggctga
    28681 aacccagcat ttgattcgac ctgagtttgg tgacgattca caaattgata tccggaaagg
    28741 gcgccatgct gtcgttgaaa aggttatggg ggctcagacc tatattccaa atacgattca
    28801 gatggcagaa gataccagta ttcaactggt tacagggcca aacatgagtg ggaagtctac
    28861 ctatatgcgt cagttagcca tgacggcggt tatggcccag ctgggttcct atgttcctgc
    28921 tgaaagcgcc catttaccga tttttgatgc gatttttacc cgtatcggag cagcagatga
    28981 cttggtttcg ggtcagtcaa cctttatggt ggagatgatg gaggccaata atgccatttc
    29041 gcatgcgacc aagaactctc tcattctctt tgatgaattg ggacgtggaa ctgcaactta
    29101 tgacgggatg gctcttgctc agtccatcat cgaatatatc catgagcaca tcggagctaa
    29161 gaccctcttt gcgacccact accatgagtt gactagtctg gagtctagtt tacaacactt
    29221 ggtcaatgtc cacgtggcaa ctttggagca ggatgggcag gtcaccttcc ttcacaagat
    29281 tgaaccggga ccagctgata aatcctacgg tatccatgtt gccaagattg ctggcttgcc
    29341 agcagacctt ttagcaaggg cggataagat tttgactcag ctagagaatc aaggaacaga
    29401 gagtcctcct cccatgagac aaactagtgc tgtcactgaa cagatttcac tctttgatag
    29461 ggcagaagag catcctatcc tagcagaatt agctaaactg gatgtgtata atatgacacc
    29521 tatgcaggtt atgaatgtct tagtagagtt aaaacagaaa ctataaaacc aagactcact
    29581 agttaatcta gctgtatcaa ggagacttct ttgacaattc tccacttttt tgctagaata
    29641 acatcacaca aacagaatga aaaggagctg acgcattgtc gctccctttt gtctattttt
    29701 taaggagaaa gtatgctgat tcagaaaata aaaacctaca agtggcaggc cctggcttcg
    29761 ctcctgatga caggcttgat ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa
    29821 gtcttaggcg ccctccttac tgggaaatat gaagttattt atagtatcgg ggcttggttg
    29881 attggtgtgg ccgtagtcgg tctagttgct ggtggactca atgttgtcct cgcagcctat
    29941 attgcccaag gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt
    30001 tcttatgctg atattgaaca atttaatgcg ggaaatctag tcgttcgaat gacaaatgat
    30061 atcaaccaga ttcagaacgt tgtcatgatg accttccaaa ttcttttcag acttcccctc
    30121 ttgttcatcg gttcgtttat cctagcggtt caaaccttac cttctctgtg gtgggtgatt
    30181 gttctcatgg tagtcttgat ttttggtttg actgctgtca tgatgggaat gatggggcct
    30241 cgttttgcta aatttcaaac ccttcttgag cgcatcaatg ccattgccaa ggaaaattta
    30301 cgtggcgttc gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc taagtttaca
    30361 gaggtctcag acgagcttct tggtcaaaac ctttacattg gttatgcctt ttcagtagtg
    30421 gaacccttta tgatgttggt tggttacggg gcggtcttcc tctctatttg gctggtcgcg
    30481 ggaatggttc agtcggatcc gtctgttgtt ggttccatcg cttcttttgt taattaccta
    30541 agccagatta tctttaccat tgttatggtt ggatttttgg gaaattctgt cagccgtgcc
    30601 atgatttcca tgcgtcgtat tcgagaaatt cttgacgcag agccagctat gaccttcaag
    30661 gatatcccag atgaagagtt ggttggaagc cttagctttg aaaatgtgac ctttacctat
    30721 ccaatggaca aggaaccgat gctgaaagat gtgagcttta ctattgaacc tggtcaaatg
    30781 gttggtgtag ttggagcgac tggtgcagga aagtcaacct tggctcaatt gattccacgt
    30841 ctctttgatc cacagaacgg ggccattaaa atcggtggca aggatattcg agaagtgagt
    30901 gaaggaaccc tgcgtaaaac agtttccatc gttctccaac gtgccattct ttttagtgga
    30961 acgattgcag ataacttgag acaggggaag ggaaatgcta ctctatttga aatggagcgc
    31021 gcagccaata ttgcccaggc tagtgaattc attcatcgta tggagaaaac ctttgaaagt
    31081 ccagttgaag aacggggaac caatttctct ggtggacaaa aacaaaggat gtcgattgcg
    31141 cgtgggattg tcagcaatcc acgtattctg atttttgacg attcgacctc agccttggat
    31201 gccaaatcag agcgcttggt gcaagaagct ttgaataagg acttgaaggg gacgacaacc
    31261 attattattg ctcaaaaaat tagctcggtt gtccatgcag acaagatctt ggttctaaat
    31321 caaggacgat tgattggtca aggcacgcat gcagacttgg ttgccaacaa tgccgtttac
    31381 cgtgaaatct atgaaacaca gaaatgaaag acaaactata agaaaagtca atagttttat
    31441 ctaaa
//
LOCUS       DAVGJJ010000012        30935 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000012 DAVGJJ010000000
VERSION     DAVGJJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.012"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            421..1038
                     /locus_tag="VVE35_000532"
     CDS             421..1038
                     /locus_tag="VVE35_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU3060778.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQVGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(1358..2245)
                     /locus_tag="VVE35_000533"
     CDS             complement(1358..2245)
                     /locus_tag="VVE35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU3060779.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(2394..4331)
                     /locus_tag="VVE35_000534"
     CDS             complement(2394..4331)
                     /locus_tag="VVE35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU3060780.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGDKVKAGDVLGTFDSNKIATAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            4706..6160
                     /locus_tag="VVE35_000535"
     CDS             4706..6160
                     /locus_tag="VVE35_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU3060781.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            6141..7106
                     /locus_tag="VVE35_000536"
     CDS             6141..7106
                     /locus_tag="VVE35_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3060782.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(7141..8415)
                     /locus_tag="VVE35_000537"
     CDS             complement(7141..8415)
                     /locus_tag="VVE35_000537"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU3060783.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(8415..9587)
                     /locus_tag="VVE35_000538"
     CDS             complement(8415..9587)
                     /locus_tag="VVE35_000538"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU3060784.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKNLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(9718..9966)
                     /locus_tag="VVE35_000539"
     CDS             complement(9718..9966)
                     /locus_tag="VVE35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060785.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(10034..10658)
                     /locus_tag="VVE35_000540"
                     /pseudo
     CDS             complement(10034..10658)
                     /locus_tag="VVE35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(10720..11169)
                     /locus_tag="VVE35_000541"
     CDS             complement(10720..11169)
                     /locus_tag="VVE35_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU3060786.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(11171..11929)
                     /locus_tag="VVE35_000542"
     CDS             complement(11171..11929)
                     /locus_tag="VVE35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU3060787.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(12044..14023)
                     /gene="pknB"
                     /locus_tag="VVE35_000543"
     CDS             complement(12044..14023)
                     /gene="pknB"
                     /locus_tag="VVE35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU3060788.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLANLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(14020..14760)
                     /locus_tag="VVE35_000544"
     CDS             complement(14020..14760)
                     /locus_tag="VVE35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU3060789.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(14775..16088)
                     /gene="rsmB"
                     /locus_tag="VVE35_000545"
     CDS             complement(14775..16088)
                     /gene="rsmB"
                     /locus_tag="VVE35_000545"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU3060790.1"
                     /translation="MTKVETARSLALAVLEDIFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(16081..17016)
                     /locus_tag="VVE35_000546"
     CDS             complement(16081..17016)
                     /locus_tag="VVE35_000546"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU3060791.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELIKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(17029..19425)
                     /locus_tag="VVE35_000547"
     CDS             complement(17029..19425)
                     /locus_tag="VVE35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU3060792.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(19491..19805)
                     /locus_tag="VVE35_000548"
     CDS             complement(19491..19805)
                     /locus_tag="VVE35_000548"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU3060793.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(19830..20456)
                     /gene="gmk"
                     /locus_tag="VVE35_000549"
     CDS             complement(19830..20456)
                     /gene="gmk"
                     /locus_tag="VVE35_000549"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU3060794.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQERLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(20586..22199)
                     /locus_tag="VVE35_000550"
     CDS             complement(20586..22199)
                     /locus_tag="VVE35_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU3060795.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(22390..22644)
                     /locus_tag="VVE35_000551"
     CDS             complement(22390..22644)
                     /locus_tag="VVE35_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU3060796.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(22648..22902)
                     /locus_tag="VVE35_000552"
     CDS             complement(22648..22902)
                     /locus_tag="VVE35_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU3060797.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(23140..24237)
                     /locus_tag="VVE35_000553"
     CDS             complement(23140..24237)
                     /locus_tag="VVE35_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU3060798.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIADLYTEQGAEMENFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGYNIKLHPIQRQGAGYHSVDKDVNFASATALRQHQRDKDFLERFMPSVALFEQA
                     SKVSWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQVRESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(24247..24993)
                     /locus_tag="VVE35_000554"
     CDS             complement(24247..24993)
                     /locus_tag="VVE35_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3060799.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(25096..25449)
                     /gene="rsfS"
                     /locus_tag="VVE35_000555"
     CDS             complement(25096..25449)
                     /gene="rsfS"
                     /locus_tag="VVE35_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU3060800.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALEVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(25462..25962)
                     /locus_tag="VVE35_000556"
     CDS             complement(25462..25962)
                     /locus_tag="VVE35_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU3060801.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(25963..26556)
                     /gene="yqeK"
                     /locus_tag="VVE35_000557"
     CDS             complement(25963..26556)
                     /gene="yqeK"
                     /locus_tag="VVE35_000557"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU3060802.1"
                     /translation="MAYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(26556..27185)
                     /locus_tag="VVE35_000558"
     CDS             complement(26556..27185)
                     /locus_tag="VVE35_000558"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU3060803.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(27237..27548)
                     /gene="yhbY"
                     /locus_tag="VVE35_000559"
     CDS             complement(27237..27548)
                     /gene="yhbY"
                     /locus_tag="VVE35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU3060804.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(27763..28869)
                     /gene="yqeH"
                     /locus_tag="VVE35_000560"
     CDS             complement(27763..28869)
                     /gene="yqeH"
                     /locus_tag="VVE35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU3060805.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRGKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(28872..29462)
                     /locus_tag="VVE35_000561"
     CDS             complement(28872..29462)
                     /locus_tag="VVE35_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU3060806.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(29414..30322)
                     /locus_tag="VVE35_000562"
     CDS             complement(29414..30322)
                     /locus_tag="VVE35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU3060807.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(30379..>30935)
                     /locus_tag="VVE35_000563"
     CDS             complement(30379..>30935)
                     /locus_tag="VVE35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU3060808.1"
                     /translation="KFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHTMV
                     QRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGAGI
                     AGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQLR
                     SEEQALHFVPNRNITVVSNFSRTD"
BASE COUNT         8514 a         7145 c         6131 g         9145 t
ORIGIN      
        1 aattaattca ctatctaact atatttagta attatttcag aactgattaa tattaaaatt
       61 aactaacgat tcaaaggatt catactagcc ataaattacg tccatcagag agagactctt
      121 actactttta gattttagtc tttctagctt cagaatacat ataaacttta gggaaaatga
      181 ctattcgaaa gcgcgaatgc ctcaaaatta tctcagataa gctattcgaa acttagaatg
      241 cttttaaatt tatggaattg cgattattcg aaacctagaa tgcatataac ctttagttga
      301 cagacctatt ctaagtctcg aagggctatt tactttctat tccttatcaa aaaagactca
      361 ttcccccttt ctcctccaaa atatggtata gtagaaatat actatctatg aggagtttac
      421 atgtcacagg ataaacaaat gaaagctgtt tctccccttc tgcagcgagt tatcaatatc
      481 tcatcgattg tcggtggggt tgggagtttg attttctgta tttgggctta tcaggctggg
      541 attttacaat ccaaggaaac cctctctgcc tttatccagc aggtaggcat ctggggtcca
      601 cctctcttta tctttttaca gattttacag actgtcgtcc ctatcattcc aggggccttg
      661 acctcggtgg ctggggtctt tatctacggg cacatcatcg ggactatcta caactatatc
      721 ggcatcgtga ttggctgtgc cattatcttt tatctggtgc gcctatacgg agctgccttt
      781 gtccaatctg tcgtcagcaa gcgcacctac gacaagtaca tcggctggct agataagggc
      841 aatcgttttg accgcttctt tatttttatg atgatttggc ccattagccc agctgacttt
      901 ctctgtatgc tggctgccct gaccaagatg agtttcaagc gctacatgac catcatcatt
      961 ctgaccaaac cctttaccct cgtggtttat acctacggtc tgacctatat tattgacttt
     1021 ttctggcaaa tgctttgaca cgtaaaaaat ccgtttggtt tcccaagcgg atttttaaag
     1081 cgtagattaa ctatagcttg atactaaata tactttggta tggaaattat gcatattttt
     1141 cgatagtgag gcgaggactt acctagcctt tccgccgtga tagaaacacc tgaaatctaa
     1201 tggtttcagg tgttcggaaa ctttgagaca ttaggatcaa agtttaggta tggaatttcg
     1261 aagaaagtcg ctaccgtccg taatcactta aggaaaggct caaaaatatt gttttcaacc
     1321 acaaaatccg tttggtttcc caagcggatt ttgtgcttta ttttgaaact tcttttgcaa
     1381 gaacaaagtt cccaagtgtg gcagaaccat ttcctgcgac tgctggcgtc acgatatagt
     1441 cacgcacatc tggtactggt aggtaaccat taagaagaga tgtaaatttc tcacggacac
     1501 ggtccagcat atgttgttga gccatgaccc ctccaccaaa gacaatcacg tctgggcgga
     1561 aagtcactgt cgcattaacc gcagcttgag cgatatagta ggcttgaaca tcccaaacag
     1621 ggttgttgag ttcaatagtt tccccacgta cacctgtacg agcttccaaa cttggaccag
     1681 ctgcataacc ttctagacat cccttatgga aaggacaaac acccttaaac tctttttcaa
     1741 tatccattgg gtgtctagca acataataat gacccatttc agggtgaccc acaccaccga
     1801 taaactcacc acgttggatg acacctgcac cgatacctgt accgattgtg tagtaaacca
     1861 agttttcgat acgaccacca gcattgttac gggcaaccac ttcaccgtaa gcagagctgt
     1921 ttacgtctgt tgtgaagtac attggcacgt ttagggcgcg acgaagggca ccaagcaagt
     1981 ccacatttgc ccagtttggt tttggagtcg tcgtgataaa gccataagtt tttgagtttt
     2041 tgtcaatatc aatcggccca aatgaaccaa ctgcaagacc agcaaggtta tcgaattttg
     2101 agaagaactc aatggtttta tcgattgttt cgattggagt tgttgttgga aattgtgttt
     2161 tttctacaac gttaaagttt tcatcaccga cagcacagac aaactttgta ccgcccgctt
     2221 ccaagcttcc atataatttt gtcatgataa acctcttgtt tttattttct ttattatagc
     2281 atacttcgaa agtctaaatg tctctatttt ttagattttc ctctgtaaat cttactatct
     2341 aataaaaacg aacaaacatg tcatttgttc gttttcacat tagagaggat tgattagatt
     2401 ttcacttcga tcacagcatc ccccttcgca actgaacctg ttgcgactgg agctactgaa
     2461 gcgtagtcag ctgtatttgt aacgataacc attgttgtat catcaagtcc agctgtagcg
     2521 attttgtttg agtcaaacgt tccaagaaca tcgccagctt tcaccttatc accttgagca
     2581 acttttgctt caaaaccgtc accgttcata gatacagtat caataccaac gtggatcaaa
     2641 acttcagcac catttcttgt tttcaaacca aaagcgtgcc ctgttggaaa ggcaattgaa
     2701 acttcagcat cagctggtgc atagaccacg ccttggcttg gtttcacagc gataccttgt
     2761 cccatagctc cacttgagaa gactgggtca ttgacatcag caagagcgac aacatcaccg
     2821 acgataggag ttacaagtgt ttcattttga agagtatcag tctcttccac tactggtgct
     2881 tctactgtag gttctgtaac tgtttctgcg gttgctgtgg cagcttcatc agcaaagaca
     2941 ctagctgctt ttgttttccc gtagaaataa gtgaaagcaa aggcggcaac aaaggacata
     3001 atttccgcaa ttatatacat agggattgaa ccagccttaa tagaaaggaa tccaataaat
     3061 ccagccgctc ccaatgaaac tgcaataacg tgagttaaac ctgctacaaa ggcaccaatt
     3121 gctgaaccag caagggcgca gaagaatggg aatttgtatt tcaagtttac cccaaagaga
     3181 gcaggttctg taataccaag gaaggcagaa acagcagccg aactggacaa tccttttgtt
     3241 ttagcatttt ttgtcaagaa gtagactgca agagtagccg caccttgtgc tacgtttgcc
     3301 attgaagcaa caacaaagat gtagtctcca aatccagtac cgttattatt gtatgcagac
     3361 aacaattgtg tttcgattgc tgggaatgat tggtgaagac ccgtcataac gattaatgag
     3421 taagttccac caaatagccc cataccaaag gcacctgttg tgttgtacaa ccatacaatc
     3481 gcatttgtaa tcgcatcaga cacaaccaac ataacaggac cgataactgt aaaggtaagg
     3541 aatcctgtaa tcatcactga taaaagcggt gtgaaagtga agtcaactgc agatggcaat
     3601 ttcttgtgga agaatttttc taaattagct aacaaccaga ctgcaacaag gactggaata
     3661 acctgataag catagtttgt ttgtgtaaca tggaaaccta agatgtccca gaaggcttct
     3721 ttaccaccca aatctggagt ggtcataatc ataccgattg ccgcaccaag gaattggttt
     3781 gctccaaagc gcttggctgc tgaaatacca accaaaacag ggaggaacat aaatggagca
     3841 gcagacatca attgaatcat agctgagaag cctttgatag caggatacat atcttcaatt
     3901 gattgaggtc caaacaaatc tggtgatgtc aagaagttcc gtaaagccat caagagacca
     3961 cctgcaacta aggcagggat aataggaaca aaaatgtctg acaaaagttt aatcaagtcc
     4021 atcaatggat tgaacttttt gtctttatta gcaatctctt tcaaatcatc tgtcgaaact
     4081 tctttcaaac cagttttctt aataatttcc gcatatacga agttaacatc accaggacca
     4141 atgataattt gatattgtcc gttagtgcta aaagtcccct tgacatccgc attattatct
     4201 agagctgctt gattcacttt tgcttcgtct ttcaatacca agcgaagacg tgtcgcacag
     4261 tgagctgcag ctacaagatt atccttaccg acagcctcaa tgacctcggc agcaacttta
     4321 ctataatcca tagcaaaaat ctccttatat aacattttgt tcttgtttga aagcgatttt
     4381 attcgccctt acgactatta ttttatcatg ttttaaaaat atgtcaagcg ttttgcagaa
     4441 aaatattcta tccacttagt gggcaagctt tagattgatt gtttttgact gtttcatcag
     4501 gaattccttg aaaaataagt ttgaaacctt ggttaaaaag tcttttattt cattatcttt
     4561 cacattttcg taaaaatttg attgatttcc tgtatttatt ttatgataat cgtttgacag
     4621 ttttttctct tttttaacat aaagggcttg cattttttat cgaaaacagt tactattaaa
     4681 agtgataaga tttttggagg aatgaatgga atggacaact gagcgtcgtt acagacttta
     4741 tcaagattgg acgcaagaag aaattcaaca tataaaggaa aatatggcac aatctccatg
     4801 gcatactcat taccatgttg agccaaaaac aggacttctc aacgacccaa atggcttttc
     4861 ttactttgat ggcaagtgga tcctctttta ccagaatttt ccttttggtg cagcccacgg
     4921 tttaaaatct tgggcacagc tagaaagtga tgatttgatt cactttagag aaactggagt
     4981 caaagttttg ccggatactc cattagatag ccacggtgcc tactctggtt ctgccatgca
     5041 atttggcgat aacttattcc tattttatac aggaaatgtt cgcgatgaaa actggatccg
     5101 tcacccatac cagatcggtg ctttgatgga caaggagggt aagattacaa agattgacaa
     5161 gatcttgatt gaccagccag cagactctac tgaccacttc cgcgatccac aaatttttaa
     5221 ctttaaaggt caatactatg ctgtcgtcgg tggacaggac ttggagaaaa aaggcttcgt
     5281 ccgtctctac aaggctgtca ataacgacta cacaaactgg caagcagttg gcgaccttga
     5341 ctttgctaac gaccgtactg cctacatgat ggaatgtcct aatctggtct ttgtagagga
     5401 acagcctgtc cttctctact gtccacaagg attggataag aaagttctag actacgataa
     5461 tatctttcca aatatgtata agattggggc ttcctttgac cctaaaaatg ccaaaatggt
     5521 agatgtgtct caacttcaaa acatggatta cggtttcgaa gcctatgcaa ctcaagcctt
     5581 caacgctcct gatgggcgtg ctctagcagt tagctggctt ggtttgccag atgtttctta
     5641 cccatctgac cgttttgacc accaaggaac cttctctttg gtcaaggaac tcactatcaa
     5701 agacgacaag ctctaccagt atccagtcgc tgctattaag gaccttcgtg cttctgaaga
     5761 agccttctca aaccgttccc aaaccaagaa cacttacgaa cttgaactca acttggaagc
     5821 taatagccag agcgagattg tcttacttgc tgataaagaa ggtaagggac tttcaatcaa
     5881 ctttgacctt gtaaacggtc aagtgacagt ggatcgtagc caggctggag agcagtatgc
     5941 ccaagaattt gggataactc gttcttgccc tatcgagaat caggctacta ctgctacaat
     6001 cttcatcgat aactctgtct ttgaaatttt catcaataaa ggagaaaaag tattttctgg
     6061 tcgtgtcttc ccacatgcgg accaaaatgg tatcctgatc aaatctggaa acccaactgg
     6121 aacttactat gaattagatt atggtcgcaa aactaactga tgtcgccaaa cttgcaggcg
     6181 tcagtcctac taccgtttct cgggttatca ataaaaaagg gtatctatct gagaaaacca
     6241 tccaaaaagt caatgaagcc atgcgagaat tgggctataa acccaacaac ctggctcgta
     6301 gtctgcaagg aaaatcagct aagttaatcg gcttgatttt ccccaatatt tccaatgttt
     6361 tctatgcaga attgattgat aaattggaac accaactctt caaaaatggt tacaagacca
     6421 tcatctgcaa cagtgaacat gattctgaga aggaacgcga atacatcgaa atgttggaag
     6481 ccaatcaggt ggacggcatc atttctggta gtcacaacct aggaatcgaa gactacaatc
     6541 gtgtgacagc gccgattatt tcctttgacc gaaacctatc gccagacatc cctgtcgtct
     6601 cctctgacaa ctatgctggt ggggttcttg ctgcccaaac cttggtcaag acaggtgccc
     6661 agtctatcat catgattaca gggaatgaca attctaattc gccaaccgga ctgcgccacg
     6721 ctggttttgc atccgtactc ccaaaagctc ctattatcaa tgtttccagt gacttttctc
     6781 ccgtcagaaa agaaatggaa atcaagaata tcttgacccg ggaaaaacca gatgccattt
     6841 ttgcttcgga tgatttgaca gctattctgg tcattaaaat cgctcaagaa ttgggcattt
     6901 ctgtcccaaa agagctcaag gtcatcggct atgatgggac ctactttatc gaaaattact
     6961 accctcaatt ggctactatc aagcaacctt tggaagagat tgcttgtctc actattgatc
     7021 ttctcttgca aaagattgaa ggcaaggaag tcgccacaac tggttacttc ttaccagtta
     7081 cgctattacc aggaaaaagt atttaaacac aagaaaactc agaccgattc gtctgagttt
     7141 ttatgatctt aaattttcga gatagcgctg ggctgtctct aggttaaagg ttttatctga
     7201 gatgaggcgc tctactaggg gagcaacttc agattcacta gccccagcta ggagagctag
     7261 ggatttggcc tgtagtttca tgtggccttg ctggatgccc gtacttacca aggctttgag
     7321 ggctgcaaaa ttttgagcaa gaccgatgga cacgataatc tgggctaatt ctctggcaga
     7381 aggatttcct agtagatcat gactgagagc tacacgtggg ttgaggccga tagagccacc
     7441 cttagtcgct acaggcatgg gcagggtcat ctcaccgacc aattcttctc tttcaaggtc
     7501 cagcgtccag cagctaagac cttgatagcg tccatctcga ctggcaaagg catgggcccc
     7561 agcttcgatg gcacgccagt cattaccagt ggcaatcaaa atcgcatcaa taccattaaa
     7621 aattccttta ttatgagtag cagctcggta aggatcagcc tgcgcaaact gactagccaa
     7681 cgcaattttc tccgcaatct ctcgtccttg atccttttgg cggctcaagt agcgaaaggc
     7741 gatgcgacag cttgcagtca ccagagaatc agtcgcgtag ttggacagga ttcccatgag
     7801 actctgtccc tgactgagtt cttctaagac tggtttcaag gcttccagca tggtgttgag
     7861 catattggca cccatggctt cctgggtatc gacatgaata taaacaacga gaaagtctgg
     7921 ttcgcctttt atctgctcga catgcagatc acgcgcccca cccccacgtt taacgataga
     7981 aggataggct tgattggcaa gctccaagag ctccgctttc ttgctggcaa tcttctcttg
     8041 cgctagttta ggattagcaa cttgataaag ggctacctgc ccaatcatct gtcgctgatg
     8101 gacttgtgca gtaaaaccac ctgcacgctt gatgattttg ctggcatagc tggccgccgc
     8161 aaccacagag ggttcttctg tcacataggg aacggtgtat tcctgaccgt tgacaagtac
     8221 ctctggaacc agtgaataag gcagagaaaa agttcccact acattctcac tcagctggtc
     8281 tgccacagtc acgctcatct gttcatcctt ctccagacta gcttgtctct caggactaag
     8341 gagcgcctga gcttttaaca gctcgaggcg ctcttggtat gattttttag aaaatccatt
     8401 ccaacttatc ttcattattt ttcaaccttg ctataacggc gttggtggtc gagaatttca
     8461 accaaggcaa aatcttgatt ttcatagcca gcaaactggg cagagtttgt ttcatccaag
     8521 tttacttcct caaaaaagac cttttcatag tctgcaacgg atagggcagt tcgttggttg
     8581 agcttgttca aacggtcttt atccaaataa gcttcatatc cttcaaccaa ttcaccactg
     8641 aagaactcag ccacagctcc acttccgtaa ctataaaggg cgattttatc cccagctttc
     8701 aagctatctg tattttccaa gagagacaaa agtccaagga aaagtgaacc tgtgtagata
     8761 ttccccacct tttgactgta gagaatagac tggtcaaaat gcttttgcaa gaggtctttt
     8821 ttctcttgag gcaagttctt atccatgatt tttttcaagc cttttagcgc taatttagga
     8881 taaggcaagt ggaaacaaac agccgcaaaa tcatccaaag taagctggta gcgtttttga
     8941 tattcaagcc aagtcgtttt caaactatcc aagtattgtt gggtagaata gacaccattt
     9001 acataaggag ttgtcgagta atttggtcgc cagaaatcca tgatgtcacg ggtctgagct
     9061 acattgtcat tattaaaggc catcatgcgt ggattttgtg taatcaacat agctacactt
     9121 ccagcacctt gagttggttc tcctggagtt tcaataccgt atttggcaat atcactggca
     9181 atgaccaaga ccttggactc cggagaattt tccacatgca atttggcata atggagggca
     9241 gcagtcgctc cgtagcaggc ttctttaatc tcgaaactac gagcaaaggg ctggatgccc
     9301 agcaagccat gcacaaagac ggccgcagcc ttactctggt caattcctga ctcggtagcc
     9361 acaatgacca tgtcaacttc ttgtctttct tgctcagtta aaatagagtc actagcactg
     9421 gccgccaagg tcacgatgtc ctcagttagg ggcgcaatac tcaattcctt gagtaagagt
     9481 cctttactta atttttcagg gtcaattccc ctcgcttctg ctaagtcttg taatttcaag
     9541 acatattgac tggtcgcaaa accaatctta tcaataccga ttgtcatatt tacctctgtt
     9601 ttatcattca tgtaaaaaat cgttctatac tattttatca caaatggcag taaaagagag
     9661 aaaaaagact tgattcacca aatcaagcct cttattggtc atcattttaa agaatgatta
     9721 gttgctagag agttcaccta tataagtagc tttataagct ccattcacag ttatcagctc
     9781 ctggaggatc aaatttcctg agtaagtcct tcccatctca tctacaaatt tttgataaaa
     9841 ctgactggtc ggaatttctc tgacatcctt atcaaatgtc ttgtcaagtg ttttactaac
     9901 cttctcagca atcaattgat gctcttgcca tccactttga aactctgagc ccgaactaga
     9961 aaccatgact gggataaaca acaaggtcag tagacttaca gacaataagg aaaatagtag
    10021 acttcctgca aaactagaat cctagttcat gattgataat accaacaatc aaattcattc
    10081 gtaatccgaa gcgtttacga tgatttcgat aggttgttga aaacatttta aacgttttta
    10141 ctttggcaaa gatattctca accttgcttc tctccttgga tagcgcatgg ttataggctt
    10201 tatcttcagc tgttagcggc ttgagtttgc tggatttacg tggagtttgt gcttgaggat
    10261 atatcttcat ggtcccttga taaccactgt cagccaagat tttaccagct tgtccgatat
    10321 ttctgcgact cattttgaac aacttcatat catgacaata gttcacagtg atatccaaag
    10381 aaacaattct cccttgactt gtgacaatcg cttgagcctt catagcgtgg catttctttt
    10441 taccagaata attcgctaat tgttttttag ggcgattgat ttttacttcc gtcgcatcaa
    10501 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat tgtaacacca cctttgaaca
    10561 agagttactt caacccattg gctccgacgg agtaagttgc tttcgtggat accaaaatca
    10621 gccgcaattt cttcataagt gcggtattct cgcacatata gaaagcgttg ttaatttatt
    10681 tatctcattt ttcagaaaat tcttttattt ctgtaaagtc tacgatactc gatgtgtttt
    10741 tatataatga tacagtctga gaatcactgt tccgctagcc attccaatag agattaccaa
    10801 agccaacatg acaaccaagg tcgcacttgc cagtgcttta ttatagtccc ctgtcacaaa
    10861 aaaggcagtt gttcggtagg agagataacc tggaaccagc ggtgccaaaa tggccaagat
    10921 aaagaccaca gcaggtgtct tataaagaat acttaaaatc tggctgacac aagaaccaat
    10981 aatggctgca atgaaggtag ctacaatgac attggtcggt tccttgagca agagatagat
    11041 tagccagaca gtcatgccca aaatccctcc aggtaagagc atagaccgtt gcacattgag
    11101 tacgattaaa aaagtgataa tggcaagaaa acttgctact gcttgtaata aaaaggttgt
    11161 tagtgtcata ttagttcatc aataccaagg cgacagaagt tcctgcccct aaagcgaggg
    11221 taatgagcag ggattcaaac atcttactca taccagagtt tatgtggttg gtcataatat
    11281 cacggaccgc attggtcaag gcaatacctg gtacaaacgg catgaccgca ccagctataa
    11341 tcaaatctgc cgttgaagga aaacctgtgt agcgagccca aaactgggca attatcccaa
    11401 agacaaaggc tccagcaaag gctgtcacaa agggaattcg gataaatttt tccacataga
    11461 gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc aagtgcgtcg tagatatttc
    11521 cgctaaacat aactgaaaag aaaggagcac taaaggtcgc agccagagtt acctgcaact
    11581 tagtataggg aaggggttga gcttgcaagg ccgtcaattg cttaaaggct gtttctaagt
    11641 caatctgccc cccaactagc tgacgagaaa tctggttcac atcgcagact ttttcgatgt
    11701 tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt ggtattttca atagagaaaa
    11761 agatagcggc aggcatggca aggacattgc aatccacaat cccctgcgaa tgcgcgattc
    11821 gaatcatggt atcttctaca cgatggattt ctgagccact tttaaggaga atagtccccg
    11881 ctagcataat cacatcaatg acggcattta attcttttga ttcttccatg ctttcctcct
    11941 tttatcaact ccctctattc tatcacaaat ctggactcaa aaaaaatctt tgccatgaaa
    12001 tcatgacaaa gattgattac tcattttgat tatccatctg cttttaagga gtagctgaag
    12061 ttgttttagg tttgtagatt gaaatcttga ctctagtctt attgaggtct accttttcac
    12121 ctgctctagg actttgttca acaaccatgc cttctgcact acctgcaggc gctgtcgtca
    12181 cttctacaac ttctatatta gcttccttaa tcccaacaat ttgaatcaaa ttgttcttag
    12241 taaactccaa gctggaacca atgtaactcg gcatggcaac acttgtaact tttttagcta
    12301 ctgtcaagac aatttgagta ggtttactca catcataagt cgttccggca cctggacttt
    12361 gtttcataat cgttcctggt tcgctttcgc tggactcttc ttcctctatc ttaatcaaat
    12421 tctcaggaac cttcttctgc ttgagttctg agattacttc tgtagagttc cgtccaatat
    12481 agttccctaa ttgaattgtc gtagcttttt tagctactgt caaaacaatt tgagttgcct
    12541 tgctcaagtc ataggtcgta ccttctggta gactttgctt caggaccgtt ccagcctcac
    12601 tctcattgga ctcttcttcc tcaattttaa tcaaattatc tggaactttt ttctctttta
    12661 attccgcgat aacatcagaa gatttccggc cgacataatt actaatttgg aaggattgtt
    12721 tgcctgatga gacaaccaaa ttgattttcg ttccttcttt tcgaccagtt ccagcgccag
    12781 gatctgtacg gataatccgc ccttcttcca ccttttcact agcctctgtc ttctcctcac
    12841 caatctcaaa attggctttt ttgagcgttg ccttggcctc tgcaactgtc tgacctgcca
    12901 catctggaat ggcaatggtt gcaggagttc tggatagtat ccaaataaga gaagctgcca
    12961 ccaatacaag gttggccaac aaaatcaggt aacgcatctt aaatctatgt tttttcggtg
    13021 cttgtggttg gtaagtttcc tctgtcacag cctggcttgg gtttttgatt gatttgtgtt
    13081 ctgtctgcgc ttgaacctta ggaatagatg tcaaggtact ctgagaaacc ttcggcaagg
    13141 tcttggtatc tgccttgctc gtttcatcaa agattaactt actttcattt ctacgattgt
    13201 aggacaagct actagacaag tccacataca tctctgaaac cgagcggtag cgattggtca
    13261 actttttagc agttgccttg ataataacat tttctaaagc ctgaggtaca gatggatttt
    13321 ctgcaataac ggacggcagg ggtttctgga aatgctggag ggcaatggtc accgcgctat
    13381 ccccgtcata agggatatgg cctgtcaaca tctcatagaa aataatcccc atggcataga
    13441 tatcactctg cacagtcgcc ttagaaccac gcgcctgctc tggtgacaag taatgaactg
    13501 agcccaacat cgagttagtc tgggtcagac ttgtctctgc aaaggctaca gcaatcccaa
    13561 agtctgtgac cttggcagtc ccatctggtg tcaaaaggat attttgaggt ttcaagtccc
    13621 tgtgaacaat tcctctggta tgggccaaac gcatggctag gaggatttgt cccatgatac
    13681 ggactgcttc ttcattagaa agaggataat gttccttgat atagcgtttg aggtctagtc
    13741 cagcaacata ctccattgca agatactgtt gaccgtcttc ctcaccaata tctgttatcc
    13801 gaacgatatg aggatggtct agatctgcca tagctctcgc ttcacgctga aaacgagcta
    13861 cagctatcgg gtccgtctgg tagttggtcc tcagaacctt cactgccact tcttccccat
    13921 ctaagattaa gtctttggct aggtagacat ccgccatacc tcctcgacca atctgtttga
    13981 caatccgata gcgtccggca aaaatcttgc cgatttggat cattctgcat cctcctcgtt
    14041 catagaaaca agggcaaccg taatgttgtc taaacctcct gcattgttag caaaacgaac
    14101 aagtgtctcc gttttatctg ctaaaggaat atcactggtt acaatatcac gaatctcact
    14161 gcctgaaatc atgttggtca agccgtcact attgagcaag agatagtcac ctgactcaag
    14221 gataactgtc ccaaaatcag gctgaatttc atctttttgc ccaatagact gggtgataat
    14281 atttttttgt ggatgagctt ctgcctcttc tggtgtcaat tgaccagcct tgagcaattc
    14341 attaaccaaa gaatgatcgc tcgtcaactg atggtattct tctccacgaa tcaagccgat
    14401 acgcgaatca ccaatatgag catagatagc ctgattatca ataatagcaa ggacttccaa
    14461 agtagttccc atgcctctgt aagcttcatc ctgaccaagc tggtgaatct tttgattttc
    14521 aatttctagg taatgggcga accattcacg cacttcattg actgtatcga tctgggtatc
    14581 aacccaagct acacccaggt ctgtgaccgc catttcacta gcgatattcc ctgcgcgatg
    14641 acctcccatc ccatcagcta aaataatcat ggtacgtcca gctctattga catagtggtt
    14701 gacatagtct tggttatttg ttcgtttctg accaacatct gttaataatg aaatttccat
    14761 gtgtcagttc cttcctaatc cgatatcttg cgaaattgac tgatgaagaa tccatcactt
    14821 ccatacaatt caggtgtaat gaggatacag ccgtctttca tgatatcctt acattcatgt
    14881 tctagtttta cctgctcgaa ctcgggatga ctctctaaaa aggccttaac gacttgaaaa
    14941 ttctcctctg agacgatagt gcaggtgcta taagttatta taccaccttt gcctagtatt
    15001 tgacaaacac tacctaatat ttctaactga atttcctgca aggacgcgaa atctgccgtt
    15061 tctttattgt atttgatatc tggttttcgg cgcaaaagac cgattcctga acaaggagca
    15121 tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact catgcacctt tctggcatcc
    15181 aatttttgag tttgaacccg atcttcaact cccagacgtt gggcattttc ttgaattaaa
    15241 tccaacttgt ggtcgtacaa gtccagagca gtaacctgac ctgtcgtaag ataagaggct
    15301 atatgggctg ttttcccacc tggagccgca caggcatcaa gcactcgctc atcaccttgt
    15361 aaatcaagcg tcggagcaac caactgactg gactcgtctt ggatggtaat ggctccatcc
    15421 gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa ccagaccagt ggttgctaaa
    15481 agggaattat tcgcctccaa caaggcttgg atttcctctt ttcgacttag gtctgttaca
    15541 cgaatactgg ctttgtttcg cactaacagg ctttcaaaga tggcttttgc tctctcctct
    15601 ccgtattctt ccttgagttt ggcaactagc caaactggga gagaataggc aatggagtca
    15661 cgcttgtttt ttcgcttgat gctagcaata tctggccagc cttcacgcaa gatacggcga
    15721 aggacagcgt tgaccaattt ttcactgcct tttttacgga gtttggccaa ttccactgct
    15781 tcattaacca cagcatgatc tggaatcttg tccaaatagc ggagttggta ggcactcatg
    15841 agaagaagga catagagcca gctgtctaac tggtctctgt cttcgataaa gtgggatagg
    15901 taccattcca gagtcagttt acgggctacc gttccataga ctagctcggt cactaagccc
    15961 ttgtctgctg cagagagctg actccccttg aggtgtttat ttaaggcgat atttgaatat
    16021 gcttggttca caaaaatatc ctctagcact gctagagcta aacttctagc cgtttctact
    16081 ttagtcacca aatcgttctc ctacggtcaa tgtacgtcca actccgttga ggaaggaagc
    16141 aatgtccatc ttaggcttac cagctggctg cacttgtttg agggatagag ccccttcagc
    16201 cgttgcgaca atcaattctt tcttgccgat agagagaatc tcacctggat ttccctgacc
    16261 ttctactggt agggcttcat aaatcttaaa gcggtcgccc ttaaggaaag tatgggcaac
    16321 aggccagggg ttcattccac gaatttggtt aaagagttga cgattggttt tgttccagtc
    16381 cagtttttct tcctctggct ttatatttgg agagaaggta acctgactcg tatcctgcgg
    16441 ttcaggtttg atatcaccag caatataggc aggcagagtg tccaaaagca aatcacgacc
    16501 aactagcgcc aatttttcaa acaaggtgcc aacattgtcc tcatctgtga tcggaatgct
    16561 gcgacgagaa atcatatctc ctgcatccat ttccttaacc atttccatga tggtcacacc
    16621 agcttcctca tccccttgaa tcaaggcata atggataggc gcaccaccac ggtgtctagg
    16681 aaggagggag gcatgaacgt tgacagcaaa gtccatgcta tcaaggagtt tgcttgggag
    16741 aaactgccca aaagcagcag tcacaattcc atctgctcct agctttataa gttcttccat
    16801 ctctggactt ccagataatt tttcaggttg gtagatagat agtcctgctt ccttggcagc
    16861 ctgcttgact ggggtttctt ggataacttt tttacgacca acagcacggt ctggctgggt
    16921 cacaacggct agaatttcgt aacggtcatc tgtcaaaagt ccttttaaga ctgttgctga
    16981 aaagtcgggg gtccccataa agattagttt tgtcatatct tctccttctt ataaaaattg
    17041 ctgcggctca tggtcaatgc tgagacggag ctcactattt tcccgttctt gagtcaaggc
    17101 caggacctgg ttgagggtcg gccccagctc atcttctaaa cggtatttaa ttaaaatctg
    17161 gtaatgatag aggttgtggg tacgggcaat cggttttggc gttggcccca gaatgggact
    17221 ggtctctgac aagcctgacc gcaaaatgtt catgacttca taggcacgtt tgaaaacctc
    17281 ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa tagtaaggtg gatagccgag
    17341 ttgtcgtctg attcccattt cataggcata aaagccttcg taatcttgat ccttggcaaa
    17401 tcgaatagca tagtgctgcg gattgtagga ctgtatcaag acttgacctg ctttttcagc
    17461 ccgacctgct cgacctgcca cctgagtcaa gagctggaag gttctctcag aagaacggaa
    17521 atcaggcaga ttcaaggccg tatccgcatt tagaactccg actagggtaa cattgggaaa
    17581 atccaaaccc tttgcaatca tctgagtacc aagtaaaata tccgcttccc ctcgcccaaa
    17641 ctggtcaagc aaggcttggt gactgccttt ctttcgagtc gtatccacat ccatcctcaa
    17701 aatgcgagct tggggaaaga gttctgctag ctcatcataa gccttctgag ttcccgtccc
    17761 atagtaacga atactgtggc tcttacagtt aggacagacc tgaggaatat ccttcgagaa
    17821 accacaataa tggcagttca tagtcttggt atccatatgc aaggtcagag aaatatcgca
    17881 gttgggacaa gtatccaccg tcccacactc ccgacacatg acaaagctag aataaccacg
    17941 gcgattgagc atgagaacca cctgctcttt tttaaccaga cggtcttgga tagcctctag
    18001 caaaggaggc gtaaagtttg acgtctcatt ttgtccgata tagtctcgaa agtcaatcac
    18061 ttgaacctca gggattgtag ccaaaggatt ggcacgttgg gttagacgta agtgttgata
    18121 gacgcctttg ccagcacgtg cccggctctc taagctcggc gttgcagatc caagtaccag
    18181 agttgcttga ttatactgag cccgtaaaat agctacctct ctggcatggt aacggggatt
    18241 gctgtcctgc ttataagccg cttcatgctc ttcatcaata atcatgacac ccagattttt
    18301 cagaggagca aagatagcag atctggcacc aacaacaact tgggcatcgc cacgctccac
    18361 cttgcgccat tcatcatact tttcaccatt ggataatcct gagtgaagaa tggctacctt
    18421 gtccccaaaa cgtgctataa aacgctcggt catctgagga gtcaaggaaa tctcaggtac
    18481 cagcaaaata gctgtcttgc ccttatccag ggcaccttgg ataatctgca agtaaacctc
    18541 ggtcttccca cttcctgtaa tcccttgaag tagaaaggga ggttgagaac tgccaataga
    18601 actcacaacc gcatcacgcg cctgtctttg ttctggattt aactccaaag gtctacttgc
    18661 ttcaattcct tcaaaataag cagccgagcg ttgaacttcc ttttggacta tggtaacagc
    18721 accttgatcc acaaagaagt tgacttgctc tcgcgagtag gactctaaca agctagccaa
    18781 ggaagcgctc tctggatgag acagcagata atctctcagt tccaactttt tcttggcacg
    18841 tgtagaaatc tcaacacctt ctaattgagc atggtcaacc tcataccaag actgggtctt
    18901 gaccttcttt tgatcgactg cctgatattc cagaccaagc aggccttttc tagtcaaacg
    18961 catcatttca gcttgcttgg caaggtctag tgaagaaaag gctagcgaat cttctgaacc
    19021 aaacaggcgc actcgttctt cctgactcaa gccttccaga ggatagagaa tcttgtcata
    19081 gctagaattc agaaatcctg gaagcatggc cttgaggata gaaattttgt aggagaagac
    19141 agatttgcgt aactcctcag ccagccagag ttgttctggc gtgagaacag gagaaaaatc
    19201 cagcacctct gcaatatctt ttaaatcttg ctccatctct tctccatctg attgggactc
    19261 caaaccaaga acaatccctt gaatcaggcg attaccctta ccaaaaggca catgaacccg
    19321 catcccaact tccagcattc cctcaaattc ctccggaatc ctgtaactat agggctggtc
    19381 cgtctgcatc aagggcacat ctacgataat cttagctagg gccatcttct cacctcctcc
    19441 ttgtcagtac attcttgcaa tagaaaaaat aagattgagt ccccccaacc ttaaattttt
    19501 tcaccatctt ctttttcttt agcaatttgc tctttgattt tcttttcttc ttcttctttg
    19561 cggcgttttt cttcttcgat acggcgacgc actgcttcac gttttccttc tggatctggg
    19621 tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc gaagagttga tttttcagac
    19681 ttgaaacctt gagttgctgg ggcacctgct tccaattcgt gggcacgttt tgcttccaag
    19741 attacgagtg aatattttga aggaaccttg tcgagcaagg tatcaataga gggttttaac
    19801 atcatttgct tgtacctatt ttctaaattt tatcgggtag ttggagattt tggtaacatc
    19861 tcctgatagt gaccaatgac acgatccaca cagaagtgtt ctgcttcaat cacacatttg
    19921 acacgttcag cagctaggga cacctgatcg ttgacaatgg cataatcata ctcacgcatg
    19981 agggcaattt cttccttggc cttttcgatt cgttgggcaa tcacttctgc actatctgtt
    20041 ccacgaccta ccaagcgttc ttgcaattca tccaaatctg gtggtgtcag gaagataaag
    20101 acagcatctg gaaccttttt cttgacctga agagcaccct gaacttcaat ttcaaggaaa
    20161 acatcgattc ccttgtccaa ggtttcattg acataggtca gaggagttcc atagtagtta
    20221 ccgacatatt ctgcgtattc caacatctgt ccttgacgaa tcagctcttc aaattcttca
    20281 cgagtacgga agaaatagtc aacaccgtcc acttctccag gacgttgtgc gcgtgtcgtc
    20341 atcgatacag agtattgaaa ttggttttca gaactctcaa aaatctctct tctaaccgtt
    20401 ccttttccaa cccctgaagg accagaaaaa acgattagta agcctcggtc tgccattgtg
    20461 tctcctttta gtcaatctgt gaaataacat ttctctagaa taatggcaaa aagccagatt
    20521 atcctttaca gtctttctat ctagtgtaac aaaaaagcag taatttttca actgctcttt
    20581 cttatttatt tagcataatc tactgcacga agctcgcgaa tcacggttac cttgatattt
    20641 cctggataat cgagattgtt ttcaattttc ttacgaactt tgtgagccaa gattgtgact
    20701 ttgtcgtcct tgatttttcc tggattgacc atgatacgaa tttcacgtcc tgcttgaagg
    20761 gcaaagctag tttgcactcc ttcaaagccg ttagcaattt cttccaaatc atggagacgc
    20821 ttgatgtagc tttcaagaga ctcactacga gcacctggac gggctgcgct caaggcatct
    20881 gctgcagcga cgataactgc tatcacgctc tcagcttcaa catctccgtg gtgactagca
    20941 atcgtattca ccacaactgg gggttccttg tacttacggg ccaattccat accgatttca
    21001 acgtggctac cttcaacctc atggtcaatg gctttcccga tatcgtgaag gaatccagca
    21061 cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca tgataccagc caacttagca
    21121 acctcaatcg aatggcgcaa aacattttgt ccatatgaag tacggaactg caaacgtccc
    21181 ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa tttcataggc agcagcctca
    21241 ccgtattcac gaatcttatt gtcaatctct tgacggtttt tctcaaccaa ctcttcgata
    21301 cgagctggat gtatacgacc atctttgagc aacatttcca tagtcatacg ggcaatctca
    21361 cgacgaatcg gatcaaatcc tgacaaggtc accacttctg gtgtatcgtc gataatcaca
    21421 tcgacccctg tcaaactttc aaaggtacga atgttacgac cttcacgacc aataatgcgt
    21481 cccttcatag tatcgtctgg cagatgaacc gttgagtttg ttgactccgc tacatattca
    21541 ccagcgatac gttgcatagc ttgaaccaag atgtccttgg ccattttgtc agaacgttcc
    21601 ttgacctctt gctcagcttc gcgaatgcga ctggcaatct ccctggtcaa gttttcctct
    21661 gtctgagcca agataatatc tcgtgcttct gcctgagaca gagcaccaat acgctctagt
    21721 tctgcttctt tttgtctttc gacttcctct aattgctctt cacgcgcatc aaggtttttc
    21781 gctctatcag aaatactttg ttctttttgt tcaagtgttt gttctttact cgtcaaattg
    21841 tcgtccttac ggtcaaggct agtagctctc tctgtcaaac gactttcgat ttgtttgagt
    21901 tcttgacgtt ctgatttaaa ttcagcgtcc acttcttcac ggtattttct ggcttcttct
    21961 ttggcctcca atagtgcttc ttttttaaga gacttgcttt cacgtttagc ttcattaaca
    22021 agtaaatccg cttcacgctc agcttgtcca cgtaaattag ttgcttcttg ttcagcattt
    22081 aaaagcatca actccgcagc ttcctgagat gatttcatct tagctgagat gctgacatat
    22141 ccaatgacta aaccaatgat gacggcaaaa acagcaatcg caagcgacat gatttccatg
    22201 tttttacctc attttattgt tattccgaat gacatacatt cttttacatt ctaccataaa
    22261 aaagtgattt tcacaaacct aaaatagaat atgttttggg gaatttggaa cacatttacc
    22321 aaaataaact tgttgtttag aaatagtagt ttagtagaga cttgagaaaa agcctacttt
    22381 gcagtagact tagtaatgat ctttaaagga caagaaagcc acgctatctc catccatcat
    22441 ataaatcaag cgattttctg catcaatacg ccgtgaccag gctccttggt aatcatattt
    22501 gagtggctct ggttttccta ttcctgtaaa tggatcgcgt tgaatatcct tgattaattt
    22561 attgattctt tttagtgttt ttttatcttg gttttgccag tagcaataat ctgcccaggc
    22621 atcttctgta aacttgagta gcattcatca ctcctcaatc acatggacct gagtgcttcc
    22681 agcacggact tgagccattc ctcgcaaaac cttgtctgat aactccttat tttgggcgat
    22741 tctcagggtt tcctgaatac tgtcccattc gctctttgaa agaactacaa tgtcctcatc
    22801 tggattttta ttgaccaccg tcaaaggctc aaattcatca tttaccttct tcatgtagtc
    22861 ctttaaatga tttcggaatg ttgagtaaag gactgcttcc ataaccatac ctcgttttag
    22921 ctcttttcca ctattataca cgaaaagaaa gaaattgtca ggaacttgta caagattttc
    22981 ttttctatct atttatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
    23041 tgtacttgag tacggcaagg cgacgttgac gcgatttgaa tttgattttc gaagagtatt
    23101 attcgtaaaa aatctcaaaa agcctacctt tcggtagact tagtttgttt ctattctaat
    23161 cggcactctt ccaaaatttt gctctgctat acttggcttt cctagttggt aaatctggtc
    23221 tgctttttgg gtcatagcat cccaaggttc tttgccaatt cggctgacta gactgacctg
    23281 acctttcaaa gacttgagat gttgcctgcc tttttcagta aatccaagga catgaatcgc
    23341 ttctggcaag ttactttctc taacctgcac caaaatatag gtcaataggc gtctgacacg
    23401 cgccttggtg taacgtttgg tagtcactaa ctcgaccaat tcttccacag actgggctgt
    23461 tttaatagct tctttaatgc gcacagccat ttcttgattg acctgataga tggtggttag
    23521 gtcgggattt gacaagattt gatagcggag caagggaaaa tagtcttccc agctcacctt
    23581 actggcctgc tcaaagaggg caacagaagg cataaatcgt tctaagaaat ctttgtccct
    23641 ctggtgctga cgaagggctg tcgccgaagc aaagttcaca tccttgtcca cagaatggta
    23701 gcctgccccc tgacgctgaa tcggatgaag tttgatgttg tacccagcaa ccgccttggc
    23761 gtaagcgagg gcaagaacat gattgggcgt attgcctgaa aaatcaagac ctgcaaattc
    23821 cttccacatg gcttgggtct tctggggata agaaagagag tcaggcaaat tttccacaaa
    23881 attctccatc tcagccccct gctctgtgta taagtcagcg attttttgat aatctagaac
    23941 ttcttctgtc ccaaaagcga ggctatctat gcccaaccga gctaagatat ccacggcccc
    24001 ttgaccgaaa aaatctgccg cctgaacgct gactaaaaag ggcaattcca ctaccagatc
    24061 cgctccattt tccagcgcca tctgggctcg tgtccactta tccacgatag caggttctcc
    24121 acgctgcatg aaattccctg acatagccac gattttcagc ccctccgctt gatccagcag
    24181 gtatttgtgt ccattatgaa aaggattgaa ctccgcgata atacccgtga tggtcatagt
    24241 aatctcctac ttctgcgcca caaaaaacca acgggtgcta gtttctgttg gctccttatc
    24301 ctcaaagtct gcatagagtt tgaaggactt aaatccagcc tgttccagca agatatcata
    24361 ggtcaagatc tcataggtcc tctcctcatg tacttcatcg tggcgactaa aggaaccgtc
    24421 agcctccttg ataaagaaag tcagctcatg cacaatggag tgaggaactt cgtcctcata
    24481 tgtatcccaa agcatggcaa aatcttccgc attttcatgg taggaatagc cagggaatac
    24541 ttcatctgtc tggtaggtcg agtgcacgtc aaagatgaaa actccctctt cattcagcgc
    24601 attgtacacg tccttaaaga cgtcccctac ttccacctca tcctgcatat agcagataga
    24661 gtccgaataa cacgtgacaa aatcgtattt ccctgccttg gacaaattca gcatattacc
    24721 ttctataaaa gcaatctttt gcttggctga agttgctctc ttctccgcaa tcttcaacat
    24781 atccgcactc aagtcaagtc cagtcacatc aaaaccagcc tgagagaagc gcactgattg
    24841 aattcctgtc ccacaagcca attccaagag tttctttctc tccttggtct taggcaaatg
    24901 acgcagagaa aaattcgtcc atttgtcgta taaactatcg tccatgaccg catcatagac
    24961 cgccgcaaag gtttcataag tcgccataat catgatacta agagctccat ggtaaacacc
    25021 actagccctt tacctttcta tcaatttttc taaaataagc aaagaaaccc agttgactac
    25081 aactgagttc atcttctaag caagagcttc tgaaatatct actgaattcg cctcatgcca
    25141 tagcttctct agattataat gggcacgcat ttcctctgaa aagatatgca cgacaacagc
    25201 accgaggtcc agtaagaccc agcctccagc tgcatcgcct tcgacatggc tacctttaaa
    25261 gcctgcttga gctacttttt cacggatatt agctgcgata gcgtccaact gacggctatt
    25321 cattgagcta gtgatgacaa agtagtccgt cacactagtc aaatcttgta cttcaagtgc
    25381 gaggatatcc tccgcacgtt tctcatcagc cgctttcacg actagttcta gtaattcttt
    25441 ttcgttcatt taatcctctt tttagaaaat atgtttgtaa tctagaacat ctgcaaagcg
    25501 ctcctcccaa atgtcctcat aaaattcatt aatcgtttct gctggaatgt catccccatc
    25561 aaaggttgtg acagcacctt ctggaacaat aagctgatag ccatattcaa aggcaacctt
    25621 gacagaggta tccacacaat attctgtctg cataccacat aaaactaatt tttcaatccc
    25681 ctgcttatcc aagtattctt ttaagccagt ttctttgaaa atactgttat acttcttctg
    25741 aaagaccttt tcagcaggtt ttcgatttaa aagcgcagat aactgccaat cttctgatgt
    25801 ttgagcttca gagttctcaa tatgttgaac atagataatt tcaatattct tgcttctagc
    25861 ctggttttgt aaataagaaa ttttttccaa tagacttttt gtctggaacc cagtttctac
    25921 taaaacattc tgaacatcaa tgattataaa agccgtcttc atttagtcct ctttcaaata
    25981 gtgcacaaag gcgttatagg tttcaagggt ttggggatag atggggaatc cctgatgagc
    26041 tagatactcc acggtacgag ctgtttcgta ggccaccgcc ttattgagcg atagacttgc
    26101 aatctcacgc gccacatcca ctccaggaaa tgcccgattg tgctcgatat agtctgcgac
    26161 gtagacaacc ttatctaggt ctgtcatctg accagcaccg actgtatgga cttcaatggc
    26221 tcgcaggatt tctgaatcat gcaaatccaa atcttcctga attttgtaaa ttccgaccat
    26281 accatgccag acattattac cccagttttt gaggccaggg tctagctggt aacggtcaat
    26341 caaagctaga aattcctgat ctgacaactt tttagcatag tcatgaagaa gacctgctag
    26401 acttgctttc tcaacatcta caccaaagcg ctgagctaat tccatagcgg cacgctccac
    26461 acctagacaa tgggttagac gtttctcagg tagaagctct gccatttttt ccaacaaggc
    26521 ctcacgggag cagttgatat agtcttgata ggccatcagt agagcccctc cttctcgata
    26581 tagtctagca caggctgagg taggagaaag ttgggtttcc gaccttgggc aaggaaggca
    26641 cgcaccatgc tggacgagat atccatgagc ggtacgtcca cccagataac tggataggaa
    26701 gtccctgcct tgtagcgtgg acgctgaacc cccacaaact gaaccatgtc aaccagttca
    26761 tcaattcggt accacttagg cagatagtca accatgtcgg caccgatgat aaagtaataa
    26821 tccgtatctg gattcttctc tgtcaaaatc ttcatggtgt cgtaagtgta ggaaataccc
    26881 ttgcgctcca actcaatagt ttcaatgact aggccgtcaa tcccctcaat tgccaactca
    26941 agcatcttga gacgatggtg ttcagggatg gtttcctttt tatcaacgtg aggaggttgg
    27001 tattcaggca tgagcagaac ttgatccagt cccaactgtt gccgtacttg atccgcaaca
    27061 ataagatggg cattgtgaac agggttaaaa ttccccccta aaatcccaac ttgtttgcgt
    27121 tttttctcct tgatttctgg ctccaactct accttggtaa agggagtcaa tagttcgatt
    27181 gtcataggct agtccccttt atttctctat aaaaacagtt atttggagtt tcggtcttag
    27241 atttctttga ctttcttaga aatcttgcga ttttctttct tgctagattg tttaaacaaa
    27301 atcaagatgc gtcctatttt ttggactgta tccacaccaa tttcttcttc caaaatttca
    27361 gctacttcgt ggatgttttc atctgtgttt tgtaagagag taaccttgat taattcacgc
    27421 gcatcaagag cttgacggac gctggttttg atttggtcgt tgagaccatt tttcccgatt
    27481 tggatgatag gtttgagggt gtgtgcctgg ctgttgagga aggcacgttg ttttgatgtt
    27541 aatgacataa tttctttaaa agagtttctt tttatacttt tctgaagtgg tgacggacgt
    27601 cagcaaagtc cttcggactt tcatgactaa aatttgagcc taaggtctca aattttccgc
    27661 agttggtaca atcacttgta ccaacttaca ccacagcgaa aagtattctc tatggggctc
    27721 gccttgctcg cccaaattca gacaaccctt tcctttctgt gcttaaataa ttgcttttcg
    27781 tgtgacgacg gcgacgcctt ctggtgccca gacggcgact tttgctatgc ctgttacacg
    27841 aatccagcct aggcctgaaa tgactaggtc tgtcttatct ttaatggtaa agacatgctg
    27901 gactagcctt gggaaatctt ctttttcctt gctatttggt ggtgtcagaa gagttcccag
    27961 gtgcttatcg tagaaagcac tagctccttc aagcttgcca cgatggagtt tgagttcatt
    28021 atcaaagaaa gcagtaaatc cttgcttttc tcctgctata aagtcaaagc gtcccaaacc
    28081 acctaaaaat agggtttgct caggattaag ctgataggtc ttaggcttga tttccttttt
    28141 aggactgaca tacttgaggt ttttggccgt caagtagtga gccatctggt ggcggtggat
    28201 aattcccggc gtatcgtaaa tataagatcc gtcgtcaagc ggaatctcta ttttgtccaa
    28261 ggttgtccct gggaagcgtg aagtagtgat gacattctga tcacccgtga tttcttggat
    28321 aatagcattg attagagttg attttccaac gttggtcaca ccgaccacat agacatcgcg
    28381 gcccttacgg tagtgttcaa tcttgtcaat gacttcctta atggcatgtt tattttgtgc
    28441 tgaagttagg accacatcga ctggacgaag accttcttca tgggcacgtt tcatgagcca
    28501 ctggctaatc ttaccagact taactgactt aggaaggata tcttttttat ttcctaccaa
    28561 gaggacatca ttgcccgaga cgaaacgtgg taaacctggg atgacagatc cattaaaatc
    28621 aaagatatca atgacattga ccactaaggc atcactgtct cccacctcgt gcaagagctt
    28681 gaggaaatca tcgttcgtca actggacatc tgtgatttca ttgtagtggc ggagacggaa
    28741 acagcgttgg caatagactt cgccagtctc caaacctttt tcaagtgccg actggggggt
    28801 aaaaccaaga ccagccttat ctgtcgtctg aatggttgct ccacaaccaa tacagagaat
    28861 ttcttccata gttaaattcc ttttttatat gtaatcggtc cgtacttttc agtgattttt
    28921 ctcataacac gacgctcacg agttcggtta atctgcgttt tgattgagtc atgttggacc
    28981 aagggtttga ctaaaattga ccgaatccct gcacggtggg ctgctcgtat atctgtcatg
    29041 agctggtcgc caaccatgac cacttccttt ttgtcatagt ggaattcctt catagcacgg
    29101 tcaataccaa atgtgaaggg cttcaaggcc cagtaaacgt aatcaatccc aaatttctca
    29161 actgctcgtt gaacgcgttt tttggtgtta tttgacacta cgataatgcc aatacccgcg
    29221 tcccgaaggt catgtagcca ttgcttcatc tctggcgttc catcagggtt gttccaagca
    29281 atgagggtat tatccaaatc gaccaaaaca gccttgattc cctgcgcctg caggcttggg
    29341 actgtcagat catagactgc ttccacagca aaatctggta tataattttc aatcgccatt
    29401 ctggctcctt ttcttaactt tttttcgcaa ttttccttag taacaaggat aaaacaatcc
    29461 acaaacctgc actagccaaa ctgatataga gccaagcatg gggctcatct gttaaaggta
    29521 agggaacatt cattccgaaa aagcctgtca cgactgccaa aacagctagc aagactgaaa
    29581 tgatagtcaa ggttgtcaaa ttgtcattca gattattgtt tagaatattg ttgtaagagg
    29641 ctgaaagctg ctgtaaaatc tgagagatta ggtctgtcat ggataccagc tgatgagcct
    29701 caatcatggc atcatcaaac tgttctctct caatctcatc aaaactacga tacaaggcat
    29761 gaccttgaat atgctctaac aaaatccgat tttgtttggc agctgccgtc agataaacca
    29821 taccagtctc caaatcagac agggcaaaga ggtttttctt ggtagttcgc tggcgcaaga
    29881 ggtcattgac ctcatcccta ctcttgtcca tctgctcaat gacaggatag taggcattgc
    29941 tgatgatttc cagactggca aagagaaact tataaatcga aagcgtgtca tggttctcca
    30001 gataacgagt catctgttca atgacatagg cgttcttggt attactaatg gtaatcaggc
    30061 gacgatgctc gacgataaag gtcatgggaa aagtctcata gtaggccttg tcctttttta
    30121 cgtctaggac attatagata aaggtaaccg tcccactttc acggtggtag tccatgtgag
    30181 cgcgctcgtt tctatccagt gcgtattcaa tggtttcctt gtccaaaccg taaatttcag
    30241 aaaggtcttc tagtttattc aactttccta ggtctaggtc tatccaggta caaccattgc
    30301 ccaactgctt ttctaaaacc atcttttctc cttattaaac tccctctatt gtaccacaaa
    30361 ttacttaaaa taacaggtct agtctgtgcg tgagaaattg ctaacaactg tgatatttcg
    30421 gttagggaca aagtgaaggg cttgctcctc gctacgaagc tgtgtcgtac gaattcccac
    30481 actgacaacc ttacccgata cagtaatcgg tccatttgtc agaacgacct catctcccac
    30541 atccagttga cgttcaaaga ggatgaaaaa gccattgatg acatcagata gaaagccttg
    30601 ggctcccata ccaatcgcta ccccagcaat accagctcca gccagcaaac tagaaactgg
    30661 caaacctaaa atcgacaaaa tgcagtagag taaaaagaaa tatagcgtat aattaaacac
    30721 attttctagt aaacgtgaga tggttttttg gcgtccgaca tcatgacgag acatttttag
    30781 agaaggtttg acaattctct gcaccatggt atgaagcatt tttttagcaa tataaaatac
    30841 aatcaaaagc agtaaaagag aaatgacctt ggttagaata ttctcgataa tggttgtcac
    30901 atctagcttt tcaatataag cctgaataaa ttttt
//
LOCUS       DAVGJJ010000013        30803 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000013 DAVGJJ010000000
VERSION     DAVGJJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.013"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            56..1948
                     /gene="pepO"
                     /locus_tag="VVE35_000564"
     CDS             56..1948
                     /gene="pepO"
                     /locus_tag="VVE35_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU3060809.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVATVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKVDVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2071..2700)
                     /locus_tag="VVE35_000565"
     CDS             complement(2071..2700)
                     /locus_tag="VVE35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU3060810.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            3694..5916
                     /locus_tag="VVE35_000566"
     CDS             3694..5916
                     /locus_tag="VVE35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU3060811.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMIELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5946..6389
                     /locus_tag="VVE35_000567"
     CDS             5946..6389
                     /locus_tag="VVE35_000567"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU3060812.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDVEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6428..6842)
                     /locus_tag="VVE35_000568"
                     /pseudo
     CDS             complement(6428..6842)
                     /locus_tag="VVE35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6861..7274)
                     /locus_tag="VVE35_000569"
     CDS             complement(6861..7274)
                     /locus_tag="VVE35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060813.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLD
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7405..8039)
                     /locus_tag="VVE35_000570"
                     /pseudo
     CDS             complement(7405..8039)
                     /locus_tag="VVE35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(8227..8877)
                     /locus_tag="VVE35_000571"
     CDS             complement(8227..8877)
                     /locus_tag="VVE35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU3060814.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            8989..9831
                     /locus_tag="VVE35_000572"
     CDS             8989..9831
                     /locus_tag="VVE35_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU3060815.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRHGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDGKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9907..10344
                     /locus_tag="VVE35_000573"
     CDS             9907..10344
                     /locus_tag="VVE35_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU3060816.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10413..10715
                     /locus_tag="VVE35_000574"
     CDS             10413..10715
                     /locus_tag="VVE35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060817.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFTFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10806..11879)
                     /locus_tag="VVE35_000575"
     CDS             complement(10806..11879)
                     /locus_tag="VVE35_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU3060818.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDAFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            12035..12712
                     /locus_tag="VVE35_000576"
     CDS             12035..12712
                     /locus_tag="VVE35_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3060819.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYTGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12705..13679
                     /locus_tag="VVE35_000577"
     CDS             12705..13679
                     /locus_tag="VVE35_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU3060820.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13728..15671)
                     /gene="thrS"
                     /locus_tag="VVE35_000578"
     CDS             complement(13728..15671)
                     /gene="thrS"
                     /locus_tag="VVE35_000578"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU3060821.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAHEIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15729..15905)
                     /locus_tag="VVE35_000579"
     CDS             complement(15729..15905)
                     /locus_tag="VVE35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU3060822.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(15912..16052)
                     /locus_tag="VVE35_000580"
     CDS             complement(15912..16052)
                     /locus_tag="VVE35_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU3060823.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(16483..16683)
                     /locus_tag="VVE35_000581"
     CDS             complement(16483..16683)
                     /locus_tag="VVE35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060824.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDSLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            16922..17551
                     /locus_tag="VVE35_000582"
     CDS             16922..17551
                     /locus_tag="VVE35_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU3060825.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVIGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17692..17961
                     /gene="rpsO"
                     /locus_tag="VVE35_000583"
     CDS             17692..17961
                     /gene="rpsO"
                     /locus_tag="VVE35_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU3060826.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18775..19389
                     /locus_tag="VVE35_000584"
     CDS             18775..19389
                     /locus_tag="VVE35_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU3060827.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19681..20430)
                     /locus_tag="VVE35_000585"
     CDS             complement(19681..20430)
                     /locus_tag="VVE35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU3060828.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQHLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20563..22899
                     /locus_tag="VVE35_000586"
     CDS             20563..22899
                     /locus_tag="VVE35_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU3060829.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DSLLLSGSFLASGSVLARVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALILKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            23428..24891
                     /locus_tag="VVE35_000587"
     CDS             23428..24891
                     /locus_tag="VVE35_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU3060830.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            24884..25312
                     /locus_tag="VVE35_000588"
     CDS             24884..25312
                     /locus_tag="VVE35_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3060831.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            25290..25739
                     /locus_tag="VVE35_000589"
     CDS             25290..25739
                     /locus_tag="VVE35_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3060832.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            25767..26078
                     /locus_tag="VVE35_000590"
     CDS             25767..26078
                     /locus_tag="VVE35_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU3060833.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            26104..27189
                     /locus_tag="VVE35_000591"
     CDS             26104..27189
                     /locus_tag="VVE35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU3060834.1"
                     /translation="MLKHLNLKGHLLTVISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            27200..27895
                     /locus_tag="VVE35_000592"
     CDS             27200..27895
                     /locus_tag="VVE35_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU3060835.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKATIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            27932..29902
                     /gene="tkt"
                     /locus_tag="VVE35_000593"
     CDS             27932..29902
                     /gene="tkt"
                     /locus_tag="VVE35_000593"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU3060836.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            30272..30803
                     /locus_tag="VVE35_000594"
                     /pseudo
     CDS             30272..30803
                     /locus_tag="VVE35_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9067 a         5621 c         6949 g         9166 t
ORIGIN      
        1 ttccctagtg atttttgtta agataaatgc aaatacaaat gaaagcgaga acaagatgac
       61 acgttatcaa gatgattttt atgatgctat caatggagaa tggcaacaga cagctgaaat
      121 cccagcagat aagtctcaaa caggaggttt tgttgattta gaccaggaaa ttgaagacct
      181 gatgctggcg acaacagaca agtggttagc aggtgaagaa gtgcctgagg atgctatctt
      241 ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt gacaagagag aagctgacgg
      301 tatcacacct gtcttaccac tccttaaaga attccaagaa ttggagactt ttgcggattt
      361 tacagctaaa ctagcagagt ttgagcttgc aggaaaacca aacttccttc cttttggtgt
      421 atcgccagac tttatggatg ctagaatcaa tgttctatgg gctagcgctc caagcacaat
      481 cttgccagat acgacctact atgcagaaga acatcctcag cgcgaagagc tcttgactct
      541 ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat ttctctgatg aagaaattga
      601 agacttgcta gaaaaaagac ttgaattgga ccgccgagtt gcgacagtgg tgctctctaa
      661 tgaagaaagt tcagaatatg ctaaactcta tcatccatat tcttacgaag atttcaagaa
      721 attcgcgcct gccctacctt tggatgactt cttcaaagca gttattgggc aattaccaga
      781 caaggttatt gtagacgagg aacgtttctg gcaagcagca gagcaattct acagtgagga
      841 agcctggtct ctccttaaag caaccttgat tttgagtgtt gtcaatcttt caaccagcta
      901 tttaacagag gatatccgtg ttttgtctgg tgcctacagc cgtgcccttt ctggagttcc
      961 agaggcaaaa gataaggtca aagcagctta tcatctagca caagaacctt tcaagcaagc
     1021 cctgggtctt tggtacgccc gtgagaagtt ctctccagaa gccaaggtgg atgtggagaa
     1081 aaaagtggca accatgattg atgtttataa ggagcgtctg cttaagaatg actggctcac
     1141 tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg atcaaacctt atattggcta
     1201 tccagaagaa ttgcctgcac gttacaagga taaggtagtg aatgaaactg ccagtctttt
     1261 tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac agttggagta agtggaacca
     1321 gcctgtagat tataaggaat ggggcatgcc tgctcatatg gtcaatgcct actacaatcc
     1381 tcagaagaac ctgattgtct ttccagcggc cattttacag gcgcctttct atgacttgca
     1441 tcagtcatct tctgctaact acggtggtat tggggcagtg attgcccatg aaatttccca
     1501 cgcctttgat actaacgggg cttcctttga cgaaaatggt agcctcaagg attggtggac
     1561 agagagcgac tatgctgcct tcaaggagaa aacacaaaaa gtcattgacc aatttgatgg
     1621 acaggattct tatggagcaa ccattaacgg taaattgact gtatcagaaa acgtggctga
     1681 cttgggagga atcgcagcag cgcttgaagc agctaagaga gaagcagact tctcagcaga
     1741 agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa ggtcgaccag aatttatgaa
     1801 acttttggct agcgtcgatg tgcacgcacc agccaaactc cgtgtcaatg tgcaagtacc
     1861 aaacttcgac gatttcttta caacctatga tgtcaaagaa ggagacggaa tgtggcgttc
     1921 accagaggag cgcgtgatta tttggtaata caaaaaatct agtcatcaga aaaaaggcat
     1981 ccaatcacgt gtgaaaacct tgataggatg ccttttgtaa tagaaaaatt tgctggatag
     2041 tttacttaaa tttcgattgt catcatcttg ctagaaaaag ggattgaagg ccttttcgtg
     2101 agcgatagta gtagctggac catgtcctgg atagacatcg tagtttggta gggtgaagag
     2161 ttgggtctgg atactatgaa ggagttgctc catgctacca gtcggaaggt cggtccgtcc
     2221 gatagtttcg cggaatagag catctcccgt caagactagg tgagcatcag gaaagactag
     2281 ggaaacacca ccgatagagt gccctggggt cggtagaacc ttaaaacgaa attcctctag
     2341 ttggtattct tcgtgaaaga caaaggtgtg ttctgcaggt tttgtgacca catctgccat
     2401 atcatcgtgg cgagggagac cggagagatt atcgacagga gtgtagagcc agctggcttc
     2461 gctctctgcg atatagacag gaggattgcc aaacgtctcg cgaaccaagt ccagactcat
     2521 gatatggtca taatgggcgt gggtcaagag aatagcacag atcggtttgt tgatcttctc
     2581 gattgtctga cgaatggctt cccaatgact accaggatcg acgacgatga ggtgcttttc
     2641 gccttctaga taataggtat tttcataggc aacaggattc acggttttat ggattttcat
     2701 actagctcca atctcaaaga atgtactaat actcttcgaa aatctcttca aaccgcgtca
     2761 actttatctg caacctcaaa gcagtgcttt gaacagcctg cggctagctt tctagtttgc
     2821 tctttgattt tcattgagta taaattaagt atagcacagt tagggagaat aggtaaggat
     2881 ttaaaatgaa cttaaaaacc agcatgacta gatgaagaca ggctggttat caatttatca
     2941 atattggaaa ggtgtcaatt gcccttcata ggttgcataa actccgtcat tagcttgttt
     3001 gatagacgtg atgtttagag tgctaccgta gttggcttct catttgtcgc tatattcact
     3061 ataagttatc ctactgggca ggttctggtc gttagcatag tattgaacga gttttttata
     3121 gctttgctgg ctgaatagga acaggcagca aactcctaac attaggagag caaagataga
     3181 aatagcagtt tttttcatag gtattctcct tataaactaa aggatagaag aaagtatctt
     3241 gaggattttt atactcttca aaaatctctt caaaccgcgt caacgtcgcc ttgccgtata
     3301 tatgtgactg actttgtcag tcttatctac aacctcaaag cagtgctttg agcaacctgc
     3361 ggctagtttc ctagtttgct ctttgatttt cattgagtat taattccaaa atccagtcaa
     3421 ggtaccagtg tatgtgatac gagttccacc gtttatcttt cgtatcttca atggaattgg
     3481 ggaaatcaga aagtctttgg gttacaatat ggtttccgtt cttctatctc tccttctatc
     3541 acctaagttt aaaatctaac ttcgtagtat attatgggcc aataattcag aattatcaag
     3601 caattttgaa ttattttttt cttattctgt tccaatctag ttttcatatt cttaaaaaag
     3661 tgataaaatg gtaggaagaa ttggagagta gagatgccga aagaagtgaa tttaacaggc
     3721 gaagaagttg tcgctttaac caaagaatat ttaacggaag aggatgttta ttttgtccat
     3781 aaggccttgg tctatgctgt tgaatgccac agtggtcaat atcgcaaatc aggcgagcct
     3841 tatatcattc accctatcca agtggcaggt attttagcta agctaaagct ggatgctgta
     3901 acagtagctt gtggattctt gcatgatgtg gtggaagata cagatgcgac cttggacgat
     3961 ttggaaagag agtttggtcc tgatgtgcgg atgattgttg acggagttac caagcttggc
     4021 aaggtcgagt acaaatcgat cgaggagcaa ttagcggaaa atcatcgcaa gatgctcatg
     4081 gccatgtctg aggacatccg cgttattttg gtcaaactgt ctgaccgctt gcacaatatg
     4141 cggaccctga aacatcttcg aaaagacaag caggagcgta tttccaaaga aaccatggaa
     4201 atctatgccc cgcttgccca tcgtttgggg atttccagtg tcaaatggga attagaagac
     4261 ttgtctttcc gttatctcaa tccaacggag ttttacaaga ttacccatat gatgaaggaa
     4321 aagcgcaggg agcgtgaggc cttggtggat gaggtagtca caaaattaga ggagtatacg
     4381 acagaacgtc acttgaaagg gaagatttat ggtcgtccca agcatattta ctcaattttc
     4441 cgcaaaatgc aggacaagag aaaacggttt gaggaaatct atgatctgat tgctattcgt
     4501 tgtattttag atacccaaag tgatgtttat gccatgcttg gttacgtgca tgaattttgg
     4561 aaaccgatgc caggtcgctt caaagactat atcgccaacc gcaaggccaa tggttatcag
     4621 tctatccata cgactgttta tggaccaaaa gggccgattg aattccagat tcgaaccaag
     4681 gaaatgcacg aggtggctga gtacggggtt gcggctcact gggcttataa gaaaggtata
     4741 aaggggcaag ttaacagcaa ggaatcagct attggaatga actggatcaa ggagatgata
     4801 gagctccaag accaggctga tgatgctaag gaatttgtgg actctgttaa ggaaaactat
     4861 ctggctgagg agatttacgt ttttacccca gatggagctg tccgttctct tcccaaagat
     4921 tcaggaccga ttgattttgc ctacgaaatc cataccaagg tcggtgaaaa agcaactggt
     4981 gccaaggtca atggccgcat ggttccactg acaaccaagt taaagacagg ggatcaggtt
     5041 gaaattatcg ccaacccgaa ctcctttgga cctagccgtg actggctcaa tatggtcaag
     5101 actagcaagg cgcgcaataa gattcgccag ttctttaaaa accaagataa ggaattgtct
     5161 gtcaacaagg gtcgtgagat gctgatggct cagttccaag aaaatggcta tgtggcaaat
     5221 aaatttatgg acaagcgcca catggatcaa gttctgcaaa agaccagtta caagacagaa
     5281 gactccctct ttgcggccat tggttttggg gaaatcggtg cgattaccgt ctttaaccgt
     5341 ctgactgaaa aagaacgccg tgaggaagag cgtgccaagg ccaaggctga ggcagaggag
     5401 cttgtcaaag gtggcgaggt caaggttgaa aataaagaaa ctctcaaggt caagcatgag
     5461 gggggagtgg ttattgaagg tgcttctggt ctcctagtgc ggattgctaa gtgttgtaac
     5521 cccgtgcctg gtgacgatat tgttggctac attaccaagg gtcgtggtgt ggctattcac
     5581 cgtgtggact gtatgaacct gcgtgcccaa gaaaactacg agcaacgtct ccttgatgtg
     5641 gaatgggaag accagtactc tagctcaaat aaggagtata tggcccatat cgatatctac
     5701 ggtctcaacc gtacaggact gttgaacgat gtactgcaag ttctttcaaa tacaaccaag
     5761 aatatttcaa cggtcaatgc ccaaccaacc aaggatatga agtttgctaa tatccatgtg
     5821 tccttcggta ttgccaacct ctctacactg accacggttg tcgataaaat taagagtgtg
     5881 ccagaagttt actctgtcaa acggaccaac ggctagttgt cctagctctt actagaaagg
     5941 ctattatgaa aatcattatc caacgggtta aaaaagccca agtgagtata gaaggccaga
     6001 ttcagggaaa aatcaatcag ggacttttat tgctggttgg tgttggacca gaggaccaag
     6061 aggaagattt ggactatgct gtgagaaaac tggtcaatat gcggattttt tcagacgtag
     6121 aaggcaagat gaacctgtct gtcaaagata ttgaaggaga aatcctctct atttctcagt
     6181 ttaccctctt tgcggatact aagaaaggca atcgtccagc ctttacaggg gcagctaaac
     6241 ctgatatggc atcagacttc tatgatgctt tcaatcaaaa attagcgcaa gaagtgcccg
     6301 ttcagacagg tatctttgga gcagatatgc aggttgagct ggttaataac ggacctgtta
     6361 ccattatcct agatactaaa aagagataag aaagaccaag cccagtcggc ttggtctttc
     6421 tcatcgatca taaaatactc caaaaagaaa tcggttcttg atatgcttgg gggaagtctc
     6481 ttttcaggct ttggcagatg cgataggaag ggatgagatg tcctaggatg aggagagttc
     6541 cctgaaggaa aagtggaata tcacttaaag acaccaagga gagaatcatc aggctatccc
     6601 ataggagaat ttgtaaagca aaacgccaga atctcggtct aatctgggaa tagagttgac
     6661 taaagtggtc acaaagttgg tttgaaagat aggtcaatag cacaggatga aagaaaatga
     6721 gccaaccact ataaatcaaa atccagtccc aagtaagccc cccttttaaa tgtcaaaaat
     6781 gccagcatga ttccatcaat gacaaggagt tgaatggttt tgatgaagat gatttttttc
     6841 atgctaaaac ctccttttcc ttagagacac tcccataaag agatgtgttt gtcgtaaaag
     6901 tctggatagt tttctcttag gtagtcagct gtcatataat aaaaagtaga atggcaagca
     6961 gtgataattg acataagaga tgaaaaacgc tgagaactaa tgaaggctaa gagaacaaaa
     7021 aatagaaaat caatggtgag aacccctaaa tagtagaagc gaatcttttt attgatttga
     7081 ggataaatct cagcgaattg atgtttgagt cggacgatca agagaaatag caggagtcct
     7141 tgatcaagga agaaaattaa accagagagc aagagccact caaaagatgt ctcgagtcta
     7201 atgataaaaa atgcaaatag tagcaaatca ataactgcaa ttgctataaa atggattcta
     7261 tagagttttt tcatgacaag acctccctct tttatctaac ttcattctac tctaaaagaa
     7321 tgggagttac aactaaaatg ataaaaatag cagagggggg attctcttaa gttggctagt
     7381 attctttatt tgagtttcct tctattatct aacttcttca tcattccaga caaataaagc
     7441 tccgattgca ttgaggatat aaaagatgta tttaccgata ttggcgaagt ttccttgaat
     7501 accagctttt gtcagctgaa cgaaattgta aatcaaccaa aagccccact gtccctcatt
     7561 gccaatgcat tcaaagcatt ggcaatgagg gacagtgcaa aggcaatagt tgttacgtag
     7621 gcaaggagat tcatcttgcc cccatatccg atatagttgg tcacaaaggc aaagaggaag
     7681 gcgatgatgg aaatgatgat ggccgccaat tttacctgtt tttggctcat ttggttgggt
     7741 ctgccttctt gcgaagcttt ccatttcttt atagcaaagg tataaatgag gaaggtgacg
     7801 ggataggtaa tgatggccgc cttatttcca aggatataat caatagcacc ggacaaaatg
     7861 gtattaacaa taccaaagta atttccccat ttgcttaatt tccccgtgaa acgagtggac
     7921 aacatggaaa tcccaacgtt ggttacggaa atcaatccaa agggtacaag agctgtccat
     7981 ggtccccagt ctacaaattt atcgaggcgt gagttgaggt aaccagatgc aatcgcaatc
     8041 caacgaccaa agcaaccccg aagaggtcaa actatttaga tgtagcaaaa atttttagtg
     8101 attttttcat aggttaaact acctttcttt ttttcaaata ttctcccacc aaatgaaagt
     8161 aaaataaaat gatagaaata aaaccctgaa aataaaactt tcagggttgg taggggactt
     8221 gagaaattag ttgattttct cgacatagag ttgttttgca atgtccatat tcacttgtaa
     8281 atcctcgtcg ttactgagga tagtgaaggt attgctgaag ccatcaaact gcttgacttg
     8341 gagctggtca ccgatgtgaa gtgagtgctt gtccagataa tggagaatgt caaaattatc
     8401 gtgcacccga gtcaggcggt aggcgccagc ttccttgata tcagctagtg ggaggttatt
     8461 gatttcaacg agtagttctc ccttggcagg aatagttcct ccgtgggggc aggttttagg
     8521 gaaacctagc agtttatcta gtctttccac gaacaggtca gagacagtgt gttccaagac
     8581 ctcagcttcc tcgtgaatct ggtcacttgt atagtctaaa tgatgaacta gaaaaacttc
     8641 aatcaagcgg tgcttacgat agagctcaga gaccagtttg agaccgaggt cagtcagtag
     8701 atagccacat tccttgtcct ttaggatgag attttcactt ttcattcgtt tgatcatttc
     8761 agttacggca gggggagaga cttgcatgcg agccgcaatt tccttgttgg taatcttatg
     8821 caggtctatg ccaatttcat aaatacattt tagatagtct tctttgtttg gggtcattcg
     8881 tttcctcttg tctaatatct ttatctagta tatcaaaaaa agctagattt ctctagctgt
     8941 gactttttat gttatactta ttgcaagaga aaaaacgagg agatggatat gacgaaaata
     9001 gctcttcttt cagatattca tggaaatacc accgccttgg aggctgtttt ggcagatgct
     9061 cggcagatag gagtggatga atactggctt ttgggagata ttctcatgcc agggacaggg
     9121 cgtagaagga ttttggactt gttggatcaa ctaccgatta cggctagagt tttgggaaac
     9181 tgggaagaca gtctttggca tggtgtccgt aaggaattgg atagtactcg ccctagtcaa
     9241 cgctatctct tgcgccagtg ccagtatgtt ttagaggaaa tttccctaga agaaattgaa
     9301 gtgctccaca atcaacctct tcaaattcat cgtcagtttg gggatttgac agtgggaatt
     9361 agccatcatc tgcctgataa gaactggggg cgagagttga ttcatactgg caaacaagag
     9421 gattttgacc gcttggtgac tcatcctccc tgtgatattg ctgtttatgg acatattcac
     9481 cagcagttgc ttcgtcacgg gactggtggg caattgattg tcaatccggg ttcgattggc
     9541 caacctttct ttttggacgc tcagctacgg aaggacttgc gggcccagta tatgattttg
     9601 gagtttgatg gcaagggcct ggtagatatg gacttccgac gggtagacta cgatgtggca
     9661 gctgaattgc agctggctaa agacctgaga cttccctatt ttgaggttta ctatgaaagt
     9721 ctggtcaatg ggatccacca tactcatcat caggaatttt tgagagaatt ggctcagaaa
     9781 gagggttgcg atcgggagtt agacgactgg ttgaaaagtg gtaacgattg acattacaac
     9841 taaaaagggt ataataatag aaaaagaaga gtagaggcag ggtagcctcg taaaaaggag
     9901 aagaggatgc aaattccaag tagatttacc attgcgactc atatgctgat aatcattgcc
     9961 ctcgagggga aggaaagcaa ggtgaccagt gattttctgg ctgctagtgt cggggtcaat
    10021 cctgtcatta tcagaaagat cttgtcccag ttgaagaagg cagagctgat ttcagtagcg
    10081 cgtggaacgg gcggaacaga gattgtcaag gaccttaagg atattagtct tttagatgtt
    10141 tatcaggcgg tcgaatgtct tggtaagaca ggtcaactct tcagtttcca tgacaatccg
    10201 aatccaaatt gccctgtagg agctcatatt catgatgttt tggatcaaaa attggagaga
    10261 attcagttga ctatggaggc agaacttggt cagaccagtc tagaaaaagt cgtggccgat
    10321 gcagagagtc agatgaagga ttaagaggga taaatgccct ctttttttct ttggataatt
    10381 atgataaaat gtagctatta aaggagtaag aaatgtatct cgttatagaa attgtattag
    10441 cctttatagt gtcattttgg aaggataata gaagtttgtg gaatccagta ttatttttat
    10501 tatccctcat ttcaagttat ttttatctat cctacctttt ttataaaaat ggatacgaga
    10561 atgttcagtt agctttttat gtatttacct ttgtcttact tccttttttg ctttttctaa
    10621 gtggcatctt tttaatctat aatggggtca ttttgttgaa gcgagaggga cgttccaagc
    10681 cccattatct ctcaatgctc tttgattttt attgactatt agattctatt taaaaagact
    10741 tagaatgcac atgaaagtat gtattctagg tcttttgttt tgtatacatt taattttcaa
    10801 gagtattatc tatttttcca tgcctggtaa cgagtatcaa tcaataactg ggtaaggcga
    10861 cccaaggttt ctctttcttc tggagtgagg gattgagctt ggacaaagag atgatgaatg
    10921 tattcaagcg cctttaagga agccaagtca ttgatagagc taataccagc atcaagaata
    10981 gtgccaaaga agaggtcgaa gtagagctga agttcttcgt cagggactgt ggccacccat
    11041 tctttaaagg ttgtgtctac ttgctggcta tcactgttgg tcttatccag ttggacgaag
    11101 tgcttgtcct caatctgcca actaaaggca tcgtgctggg cgatgccacc cagggcagta
    11161 ctctgaacga tgatttggtg agcaggaatt tccagcatca taccgataat ggaaccttgt
    11221 ggaatgaata tcttgcttct atccattatc ctttgataac ccgcagtctg tgtcaattct
    11281 tgatggagac caggtgcatc aaatgtataa actgctgtga tctgattttg caaactttgc
    11341 tcaatttggc tagcagcata gatagcgaga tttcctccct tggaatgccc agctagaata
    11401 accttttgct taggatgatg ggcaaaaaag ttctttaaat agcgaagggc gtgcttttga
    11461 gcaggaattt ccttcatata ggtcaggtgg aaatcttcct tccagccaat gatactgtca
    11521 tctgtcccac gaaagacaat cagataggta tcgaggctga cacgataagt catagccgca
    11581 aattgctttt gcagttcagg gtcgatgtcg ttgataaaat gggagagttt gcaatttttg
    11641 aagcgcttgt gttgagccaa ttcatctaat aattgaaggc gatttttgct agtaagcatg
    11701 gtgggatctc ttggaacctg aggtgctaga tctaaaagac gttgaggaag tgtggagacc
    11761 agattatcaa aggagaggta ggtgatttct attaaggtta gaatgtctaa ctcattcaag
    11821 ggaaggtcgt aataagaatc atatgcgaca tctttcagat agtcaaaaat attggccata
    11881 agagctcctt tctttttatt tatcttagca aatttccctc aaattagcta gtagcatagc
    11941 ctgtttgtac tggctaaaaa caggctattt caaattcagt ttcagaccat ctagcatgga
    12001 aaaatctgtt ataataatgg aaaaggagaa gcgcatgcac aagattttat taatagaaga
    12061 tgatcaggtc attcgtcaac agattgggaa aatgctctct gaatggggat ttgaagtggt
    12121 cctggtagaa gactttatgg aagttttgag tctatttgtt cagtcggaac ctcatctggt
    12181 cctcatggat attggtttgc ccttgtttaa tggttatcac tggtgtcagg aaatccgcaa
    12241 gatttccaag gtacctatca tgtttctttc ttcgagagac caggctatgg atattgtcat
    12301 ggcaatcaat atgggggcgg atgactttgt gaccaagcct tttgaccagc aggttctttt
    12361 agctaaggtt cagggcttgt tgcgtcgttc ctatgagttt gggcgtgatg agagtttgct
    12421 ggaatatact ggtgttatcc tcaataccaa atccatggat ttacattatc aagggcaagt
    12481 cttgaatttg accaagaatg aattccagat tttacgcgtg ttatttgagc atgcaggcaa
    12541 catcgtagca cgtgacgacc tgatgcggga actttggaac agtgactttt tcattgatga
    12601 taataccctc tctgtcaatg tggctcgttt gcgtaaaaag ttggaggagc agggattggt
    12661 aggatttatc gagaccaaga aaggaatagg gtacggattg aagcatgctt gattggaaac
    12721 aattttttct agcctatctg cgctcccgta gtcgtctttt tatctatctg ctttctttgg
    12781 catttcttgt cttactcttt cagtttttat ttgccagtct aggaatttac ttcctctact
    12841 ttttcttctt gtgttgcttt gtaaccatct tatttttcac ttgggacata ttggtggaaa
    12901 cgcaggtcta tcgccaggaa cttctctatg gagagaggga agccaagtct cctttggaaa
    12961 tagctttagc agaaaaatta gaagcgcgtg agatggaact ctatcagcag aggtcaaaag
    13021 cagaaagaaa actgacggat ttgctggatt actatacctt gtgggtccat cagataaaga
    13081 cccccattgc agccagtcaa ctcttagttg cagaagtggt cgaccgccaa ctgaagcagc
    13141 agctagaaca ggaaattttc aaaatcgact cctataccaa cctagtttta cagtacctgc
    13201 gtttagaaag tttccatgat gatttgctct taaagcaggt tcaaattgag gacttggtca
    13261 aggaaataat tcgtaaatat gctcttttct ttattcaaaa aggcttaaat gtcaatctac
    13321 atgaccttga taaagaaatc gtgacggata aaaagtggct gctagtggtt attgagcaaa
    13381 tcatctcaaa cagtctcaag tacaccaagg aaggtggtct ggagatttat atggatgacc
    13441 aagagctttg tatcaaagat acgggaatcg ggataaaaaa cagtgatgtc ctccgagtat
    13501 ttgaacgtgg cttttcagga tacaatggcc gtttgaccca gcagtcctct ggacttggcc
    13561 tttatctatc taagaaaatt tctgaagaac tggggcacca gattcgtatc gagtctgagg
    13621 tcggaaaagg aacgacagtg cggattcagt ttgctcaagt gaacttagtc cttgagtaaa
    13681 aatagatgag aaaaagcctc cagattggag gcttttatgc tagactctta tttctcaacg
    13741 cgtgatttgt tggcgatatc agctaggata gcttgaacaa aattatcaac tgagacagtt
    13801 tgtgtttctt tttggccgta gcgacgaacg ttgactgttt cgtcttccat ttctttgtct
    13861 ccaacaatta attggtaagg aatcttgctg gtttgtgaag cacgaatctt gaactgcatt
    13921 ttttcattgc gctcatctac gtctgcacgg acaccgcggt cacggagttt cttggccact
    13981 tcccaagcgt agtccacgtg tttttcgtta gatactggga tgagggttac ttggtgtggt
    14041 gccagccatg ttgggaaggc ccccttgtag ttctcaatca agatagctgt gaagcgttcc
    14101 atagttgaga taaccccacg gtggatcatg actggacggt gatcttcgcc atcagctccg
    14161 atgtatttga ggtcgaagcg ttctggcaac aagaaatcaa gttggatagt agaaagggtt
    14221 tcttcttttc caagggcagt tttaatctgg atatccaatt ttggtccgta gaaggctgct
    14281 tcaccttcgg cttcaaagta gtccacgccc atttcatcaa gagctgcacg aagcatggtt
    14341 tgggcatttt cccacatctc atcgttatca aagtacttat gagtatcttg agggtcacga
    14401 agagagaggc ggaagcggta gtcagtcaag ttgaagtctt cataaacatc gataatcaac
    14461 tgaagggcac gttggaattc ttcttggatt tgttctggag taacgaatag gtgaccgtcg
    14521 ttgagtgaca tttcacgtac acgttgaagg ccagtgaggg caccagattt ttcgtaacgg
    14581 tgcatcatac cgatttcagc gatacggatt ggcaattcac ggtaagagtg aacatggtgt
    14641 ttaaaaactt ggatgtggtg cggacagttc attggacgaa ggacaaattc ttccccgtca
    14701 cccatgtcca tggttgggaa catgtcttct tggtaatgat cccagtgacc agaagtcttg
    14761 taaagctcaa cagaagcaag tggtggagtg tagacgtgtt ggtagccaga agccaactct
    14821 ttgtttacga tgtagcgttc caattcacga cggatagtcg caccatttgg taaccagaat
    14881 ggcaaacctt gtcccacttc ttgtgaaatc ataaagaggt caagctcttt accaagttta
    14941 cggtggtcac gttccttagc ttcttcacgc atttgaaggt agtttttcaa gtctttcttg
    15001 tcaaaccaag ctgtaccgta gatacgttgc atcatagcgt tgtcgctgtt tccacgccag
    15061 tacgcaccag ctacatggag aaggtggaag atttggatac gacctgttga tggaacgtga
    15121 ggtccacggc agaggtctac atattcaccc tgacgataga tagtcaaacc gccttcgtct
    15181 tctgagtgtt cttcaatcaa ttccaacttg taagggtcat ttttgaagat ttcatgtgcc
    15241 tcgtctttag tcacttcttc acgaatagat gggaagtttt ctttgacgat tttttgcatt
    15301 tcttcttcga tacgaggaag gtcttcgtta gagatttgac cagctgtgtt gtcagtatcg
    15361 tagtagaaac catcttcgat ggctggacca actcccaagt gaatgtctgg gaaaagacga
    15421 cgagctgctt gggcgaacaa gtgagctgct gagtgacgca agattggaag ggcatcttcg
    15481 tgatcaggtg tcacaatttc gatgcttcca tcttcagtga tagcgcgagt agtgtcgatg
    15541 agtttgccgt tgaatttacc agccaaggct tttttagcta gggaattgct gatagattgg
    15601 gcaatttcaa aagttgttac gccagattcg aattcacgaa cagcgccatc tgggaaagta
    15661 atgttaatca tgatgttctc cttttttatt atttcatagt ttctaagcat tgatttttta
    15721 tcagcagact acttccgagt cctaacgttc aattgctttt ctttatcaga gaggaagttt
    15781 tccatttccg tcagaatgtc tatatccaaa cgttgagaaa gttctaatag ccaccagata
    15841 ttttctgaaa gtttttgttc cagtgtgtag ggtgtttcat catagtagcg tccttgcttt
    15901 gtcatcacca gtcattggaa atttccaata tcattagata aagccaagag gtcttcttct
    15961 accgtccact tgaaatcatg atgcttaact tccagttcgt gataagcttg tcggattgcc
    16021 caactgcgct ccactaattc ttgcaactcc ataattagta tcctaataaa aaaatgcgac
    16081 atcctcgaaa ggacgtcgca acgtggttcc accttcattt atgtacctca aaagagaggg
    16141 acacctctga tcggctctaa cgtggccacc gttttatgtt ttcataaaaa actcaagagt
    16201 agtatcagtt tagacttcct atgcgtttcc agcaaccacg cactctctag tagaaaggga
    16261 ctaagtgact tgtctctatg gattattata gcatgattgt gagatttttc aaggattttg
    16321 tgaaagtttt ttgtttttca ttttttgtag aatatgcttg atgctaatac cagaatccat
    16381 gcccaccaga gcaactgttt catagattag caagtaaatt agtataaact caccaaaggt
    16441 tatgatagta aagtaaaaga gcagtccgtt accaactaac catcaaagtg agaaacggaa
    16501 acggtagcta tagagtaacg atacaatcaa gattaccaga ggtgtaaaga agagaagatt
    16561 gtttatatag agtcccttga gtgtaaggga gtcagaaaca actagtaaga acaactcata
    16621 aagtggccaa taaaagaata atatgaccag atagtagggg agatgggaat agaatctacg
    16681 catgataatc acctcgtttt aaaaaaacag acaacttaga tctcttgcta tgattattat
    16741 agcatgttta aaacaagaaa gtaaatttgt tgacaaagaa gatttttctt ttcactagaa
    16801 tcatggttag ttttcttggt agaaatattt tgataataat taagcaagac tattgacatt
    16861 gattattaag agtgtcacaa ttgttgtaac taaaataatt acaacaagag aggtataaaa
    16921 aatgaaacta gcagttattg cagcaaatgg acaagcgggg aaggcaattg ttgaagaagc
    16981 agtcaaacga ggacatgaag taacggctat tgttcgttct gaaaacaaaa gtcaagctga
    17041 gtctattatt aaaaaagatt tatttgagtt gacaaaagat gatcttactg gatttgatgc
    17101 tgttatttca gcatttggtg cttatacacc agatacgcta ccgctccaca gcaaatcaat
    17161 cgaactattt aatcagcttc ttgctggaac acagacacgt tttctagtta tagggggatc
    17221 aggaagcctt tatatagatg aaacaaaaac gacacgactt ttagatacgc ctgattttcc
    17281 tgaagaattt aagtcactag caaaagctca agcggatgaa ttggatcttc ttcgtacaaa
    17341 aaataatctc aactgggctt ttgtcagtcc tgctgtagat tttattcccg atggtgaaaa
    17401 aacaggaaac tacattctag caggagaaat ttttactact aatgaaaaag gaataagcca
    17461 gattagttat gcagattatg ctattggatt ggtagatgaa cttgaaaaag gtcatcatat
    17521 aaaagaacgt atctctttac ttgaaaaata ataactatga gagtattaat gtttgacaga
    17581 aaatttaaat cttggtagaa tagggattgt cgtaaagaca aataacttct tcttggttac
    17641 aggcatgcca acctgtcact cggatgaagc caaataaaaa ggagaaacat catggcaatc
    17701 tcaaaagaga aaaaaaatga aatcattgca caatatgcac gtcacgaagg tgatacaggt
    17761 tcagtagagg ttcaagttgc tgtccttact tgggaaatca accaccttaa cgaacacatc
    17821 aaacaacaca aaaaagacca cgctacttac cgtggattga tgaaaaaaat cggtcgccgt
    17881 cgtaacttgc ttgcatactt gcgtaaaaac gacgttaacc gttaccgtga gttgatcaac
    17941 tctctaggac ttcgtcgcta attcaagata caaaggccgt caaaagcaca aagcaaaaat
    18001 aggaaaattg acgaagaaac ttcagtttct aggagatttt atcttttttg ccaagtgctt
    18061 aggccgtgtt caattgagca tatcttgata ataaagctac tctaaattgg gtagctttgt
    18121 tgcgttgtca ccttacctcg ctatactcaa gtataatctt cggttacggt tcctagcact
    18181 gtaaggtaaa ataaactagc ttttctccct tcggggagat ttttttgttt cactgaaagt
    18241 ttcgtacgat cttcgcctcg tcgcctagtc tataaacgat ttatctagca agaatcatga
    18301 tgctaagggc gtcaaaaatc cgtatgaaaa tagggaaatg acacagtgtt cgatgagcac
    18361 aaggagtttc atctttttca ctaggatttt agcccgggct aaaatcagct ctctgacttc
    18421 agagggcttt tttattgagg ttttatcggt aacaattttg gagactacaa aaacctcaaa
    18481 tggtatcagc taattacacc ataaaggtgc gcagttactc ggcttttcaa gccgagtagc
    18541 tgtatgcaag ccccctagaa gagcccactc tttacaaagt aaagtatagt atgttatact
    18601 ttacatggaa gtagtcactg aattccagtt agaaattact ttgtaactac gttttgagga
    18661 ggagtaaaat gctttcctac gttcgacatt acccactagc gatagctaaa ttaatgtgtc
    18721 tgtgctctcc taaaatctgc tgatttatta ctgactaata caggaggttt ttttatggga
    18781 cagacaatca tatctgctat tggtgtttat atttccacca gtatcgatta tttaattatt
    18841 ttaattattt tatttgcaca gctatcacag aataaacaga aatggcatat ttatgcgggg
    18901 caatatctag gcacaggctt acttgtaggg gcgagtttag ttgctgctta tgtcgttaat
    18961 ttcgtgcctg aagaatggat ggttggattg cttggtttaa tccctatcta tttagggatt
    19021 cgctttgcaa ttgttggaga agatgcggaa gaagaagagg aagaaattat tgaaagatta
    19081 gaacaaagca aggcaaatca actgttttgg acagttacat tgctgacaat tgcgtctggc
    19141 ggagataatt taggtatcta tataccttat tttgcttcgt tagattggtc acagaccctc
    19201 gtggccttgc ttgtgtttgt aatcggcata attatctttt gcgagattag tagggtgtta
    19261 tcctctattc cgttaatatt cgagacaatt gaaaaatacg agcgaatcat tgtgccctta
    19321 gtattcattc tacttggact atacatcatg tatgaaaatg gcacgataga gacttttctg
    19381 atcgtgtaga tttttttgtt tcactaggat tttagcccga gctcaaatca gctctctgat
    19441 tttcagaggg ctttttcatt gagtataaat ggacaatctt aaagttactc tattgtagag
    19501 tggcttttga tttgattagg agattcttat aaatatagta aaatgaaata agaataggac
    19561 aaatcgatca ggacagtcaa atcgatttct aaaaatgttt tagaagtaga ggtgtactat
    19621 tctagttcca atctactata taaatgaaaa ccttagctgt ttggccaagg ttcatttctt
    19681 ctatgcaagt tcttctcttt tcttatctgg gttccagatg aagcttgcga taaagctaaa
    19741 gatgatggtt aggatagcga ggataatagc aaggatgagg gcagggatgc ggataaacca
    19801 ccagagtgga tttggttttt tatcattgtg ccattgtttc gtttcttcgt ccagatgttg
    19861 gaattctgtt tgaatacgat tggcgactgt ttcaatgcct tcatcattca ttttcttgat
    19921 atctgagata tcgattggat ttccaaagtt catatcgaca cgttcacggc taatcaagcc
    19981 cttcaaagtc atgggaccgg tgtaggtaac cggcatgata cggaccttgg ccattttggc
    20041 aatcagtgct gcgcccccct tgacatcgtt tgagtggcgg ctaccacttg gaaacatgat
    20101 gagagaacgg tcactttttt tgagaacgtt gataggatat ttgatggctg aggcgctggg
    20161 attttcacgg tcgatgggaa aggcgccaca catacgaatc caccaaccaa agatacggtt
    20221 ggtaaagagt tctttttttg ccataaagat gaactgtttt ggcttggtcg caaaggccat
    20281 ataaacagga tcccaccagg tacggtgagg cgcaactaaa atataatttt catcttgatt
    20341 aggaatttta tcagtattat gatagtgagc attgccattg atggaccata agagcaatac
    20401 aactaatcca cgcaaataag tataaaacat gcgatctcct tcgattgttt tcttgttatt
    20461 attatacctt atcaaaggag ggctggcaaa cttttccctt gactagatgc atatttggga
    20521 tgaaattaga attctgttaa aaaaaatgat ataatagaat ttatggataa aaataagatt
    20581 atgggattaa cccaaagaga agtcaaggaa agacaggctg agggtttggt caatgacttt
    20641 accgcatcag ccagtaccag cacttggcaa atcgttaaac gaaatgtctt tacccttttt
    20701 aacgctttga actttgccat tgctttggcc cttgcctttg tgcaggcttg gagcaatctg
    20761 gtcttctttg ctgttatctg ctttaacgct ttttctggga ttgtgaccga gctacgagcc
    20821 aaacacatgg tggacaagct caatctcatg accaaggaaa aggtcaaaac catccgtgat
    20881 ggtcaggaag ttgctcttaa tcctgaagaa ttagtgctag gagatgtcat tcgtttgtct
    20941 gcaggagagc agattcctag tgatgccttg gttttggaag gctttgcgga agtcaatgaa
    21001 gccatgttaa cgggagaaag tgatttggtg caaaaggaag ttgacagctt acttttgtca
    21061 ggaagtttct tggctagtgg gtcagtttta gctcgtgtcc atcatgtagg tgcagacaac
    21121 tatgccgcca aacttatgct ggaagccaag accgttaaac ccatcaactc acgtatcatg
    21181 aaatcgctgg acaagctagc aggttttact ggaaagatta tcattccttt tggtctggct
    21241 ctcttgctag aagccttgat tttaaaaggc ttacctctta agtcttccgt tgtaaattcg
    21301 tcaacagccc ttttggggat gttgcccaag ggaattgccc ttttgaccat tacttcgctc
    21361 ttgactgcag tgattaagct tggcttgaaa aaggtcttgg tgcaggagat gtactctgtt
    21421 gagaccttgg cgcgcgtgga tatgctctgt ctggacaaga cgggtaccat cacccaagga
    21481 aagatgcagg tggaggctgt tcttccactg acggaaactt atggtgaaga ggctgttgcc
    21541 agcatcctga ccagctatat ggcccatagt gaggataaaa atccaacagc tcaagccatt
    21601 cgccagagat tccaaggtca agtagtctat cctatgctct ctaatcttcc cttctccagc
    21661 gaccgcaagt ggggagccat ggagttagaa ggtttgggaa tagttttctt aggggcacct
    21721 gagatgttgc tggattctga ggtcccagaa gccagagagg ccttggagag aggttcacgt
    21781 gtcttggtct tagctctcag tcaggagaaa ttagaccatc acaaaccaca gaaaccatct
    21841 gatattcagg ctctagcctt gctggaaatc ttggatccta ttcgagaggg agcggcagag
    21901 acgctgaact atctccgttc tcaggaagtg gggctcaaga ttatctctgg tgacaatcca
    21961 gttacggtat ccagcattgc ccagaaggct ggttttgcgg actatcacag ctatgtcgat
    22021 tgttcaaaaa tcacggatga ggaattgatt gctctggcgg aggagacagc gattttcgga
    22081 cgtgtttccc ctcatcaaaa gaaactcatt atccaaacgc tgaaaaaagc gggtcataca
    22141 acagctatga ctggggatgg agttaatgat atcctggctc ttcgtgaggc ggattgttct
    22201 atcgtaatgg cggagggaga cccggcaacc cgtcagattg ccaacctggt tctcttgaac
    22261 tcagacttta atgatgttcc tgagattctt tttgaaggtc gtcgtgtggt caataacatt
    22321 gcccacatcg ctccgatttt cttgataaag accatctatt ccttcctgtt agcagtcatc
    22381 tgtattgcca gtgctttact aggtcggtca gagtggattt tgattttccc cttcattccg
    22441 atccagatta ccatgattga ccagtttgtg gaaggtttcc caccattcgt tctgactttt
    22501 gagcgaaata tcaaacctgt tgagccaaat ttcctcagaa aatccatgct tcgtgcccta
    22561 ccaagtgccc tcatggtcgt gtttagcgtt ctttttgtga aaatctttgg aacgagtcaa
    22621 ggttggtctg agttagaaat ctcaactctc ctctattatc tcttggggtc aattggtttc
    22681 ttatccgtat ttagagcctg catgccattc actctatggc gagtcctctt gattgtctgg
    22741 tcagtaggag gcttcctagc cacagctctc ttcccaagaa tccaaaaact gcttgaaatt
    22801 tcaaccttga cagaacaaac gttgcctgtt tatggtgtca tgatgttggt ctttacagtg
    22861 attttcatcc tgactagtcg ctatcaagct agaaaataaa gaaagactgc aatctgtgga
    22921 ttgcagtttt ttatacgatg gatctatcgt agatctgatg tgcaaggcct acatgtctat
    22981 tatctatcgg tgaacccaag agtgattctc aagcctgctt ggattgaggt aatagattta
    23041 aatatctgta gttagactat ttgaagtttg atgtaagaaa gagaaagcga cagattgaag
    23101 taattttaac tctcttctat tgctagaaca aatggtcgga taggttggta gtttgaaaat
    23161 gaagatgcta tctattgtta aatggaacat agtgttattt attagaaaat cgtttggttt
    23221 atttcttatc aaatacgaaa agcaacttaa atatttcaac taaaatagat gttatgaaga
    23281 aaaggtaaaa tgattttggt atagtgaggt tctgttctat ttgatatcat atttttgata
    23341 aaaacaaaaa tgtccattgc aaaggacaaa atgcgaagta tattattttt tgaaagcgat
    23401 ataatggaat cataaaggag gtgtatcgtg tctagaaaac aagaacaaat ggaaacgttg
    23461 ttgctccttt tgcgagatag taaggattat atatctgcta aagtattggg agaaaaatta
    23521 aattgctctg ataaaacggt ttatcgcctt gtcaaggtaa tcaacaaaga ttgtccggta
    23581 gaagcattca ttttatctga aaaaggcaga ggtttcaaat taaatccaag aagttccctc
    23641 gtggacgttg atgggaattt tacagaggct tttgatcctg aagtaaggcg tgaaaaatta
    23701 ctagaacgtc tcttgttgac tgctcctaag ccacattcta tttatgattt aggagaggaa
    23761 ttctacgtaa gcgagtcagt agtactaaaa gatcgtcaga tattacaaga gagtctagca
    23821 atttatgggt tagatttaaa aatgagacaa cgaaagcttt ttattgatgg ggatgaggct
    23881 caaattcgtt cagccattct aaatctactg ccaatgttta atcagttgga tttagagcaa
    23941 attacacaga ataaggttca gcctcttgac ggagaacttg ctcacttttg tttgggatta
    24001 ctgattacac ttgagagaga attgggggta aacattccct atccatataa tataaatatt
    24061 ttctctcacc tgtatatttt tatcagtagg aatcgtcgta gtactagtat tcatgttgta
    24121 gcaccttcaa aacctactat tgttgatgag aaaatttaca gtgtctgtca aaaaattatt
    24181 caagaaattg aacaatattt taagatgaag gttgatgcag ttgagattga ctatctttat
    24241 caatacgttg tatcttcgag attgcaaaaa ccattttctt ccgggaagct tcctttttct
    24301 cagcgagttt tagatgtcac tcattactat tttagccgta tgtgtatgga caatagagag
    24361 attgaaacga cagatcctga ctttgttgac ttggcgagtc atatcagtcc cttactgagg
    24421 agattagata atagagtaca gattaagaat agtcttttat cacaaattct tttaacctat
    24481 cctaatctgg ttaaagagtt aacaactatt tctaaagaag tgagtctagt atttggtttt
    24541 gcgtccttga gtctggacga gattggtttt ctagtcttat attttgcacg gtttcaagaa
    24601 aagcgagcac gtcctctaaa aacagtagtg atgtgtacat caggtgtcgg aacttcagag
    24661 cttttacgag cacgattaga aaagcaattt actgaattgg atattattga tgtagttgct
    24721 tatcatcaat tagatgagct gataaatcta tatccagatt tagatttcat tgtgacgacg
    24781 gtagctttgc aggaaccagc aagtgtcccg tttgtcctag ttagtgtttt tctaaccgag
    24841 ggtgataaac aacgtcttca agcaaaaatt caggagataa actatgaata atctttcgct
    24901 tgtccttatg gatatatctg ttcaaaatcg tcaagaagcc tacaaagaat tagcaaatca
    24961 aatcagcctt cttgtttctg aagatacaga aaaaatagaa gagcttctat attaccgtga
    25021 gagacaggga agtatagagg ttgctaaagg tgttcttcta ccacattgtg aaggaaactt
    25081 tcaacatcat gtcttagtga ttactagatt aaaatcacct atcagagaat ggtcgaagga
    25141 tatccagtgt gttgacctta ttatcggttt ggccattgca gtatcacagg acaagtcatg
    25201 tattaaaaca ttgatgagaa gactagcaga tgaatcattc ataaatcaat taaaacagtt
    25261 aacaaaagaa gaattacggg agataatata tggaaatcaa agatattctt aatgtgagtc
    25321 tgatccagac ggatttacag atgcagagca aagaagaggt ttttgaggca ttagctcaac
    25381 tattggttga gacgggttat gtgtctgata gagaccaatt tatcgaaggt ctttatcaga
    25441 gagaggcaga aggacagacc ggtattggga attatattgc tattccccat agcaagagtt
    25501 ctgctgtgga gaaggcgggg gtagtcatag ctataaatca caatgagatt ccttgggaga
    25561 ccattgatgg gaaaggggtc aaagtaattg tactctttgc agttggtgat gatacagaag
    25621 ctgctaggga gcatttgaag accttatcac tctttgctcg aaaacttggt aatgacgaag
    25681 ttgttgccaa attagttcgg gctcagacat ctgatgatgt gattgcagct ttttgttaat
    25741 aagaaaaaat tttggagggt atccgtatga aaattgttgg tgttgcagct tgtactgtgg
    25801 gaattgccca cacttatatt gcacaggaaa aattagagaa tgccgcaaag gtagctggac
    25861 atgtgattca tgttgagact caggggacaa taggggtaga aaatgaattg agtcaagagc
    25921 agattgatgc agcggatgta gttattttag cagttgatgt taagatttct ggtatggaac
    25981 gctttgaggg taaaaagatt atcaaggttc caacagaagt ggcagtcaaa tctcccaata
    26041 aactgattgc taaagctgtt gagattgtta cgaaataact gaaaatattt aaggagaaaa
    26101 tatatgttga aacacttaaa cttaaaaggt cacttattga cagtcatttc ctatatgatt
    26161 ccaattgttt gtggtgcagg attcttagtt gccattggtt tagcaatggg gggtggtgtt
    26221 cctgacgctc ttgtagcagg aaaattcact atctgggatg ctttagcaac tatgggtggt
    26281 aaagcccttg gtctcttgcc agttgttatt gctacaggtt tgtcttactc gattgctggt
    26341 aaaccaggga ttgcaccagg ttttgttgtt ggtctaattg ccaattctgt tggttcaggg
    26401 tttatcggtg gtatcttggg aggttatata gctggtttct tggttcaagc gattattaaa
    26461 aaggtcaaag taccaaactg gattaaaggt ttaatgccaa ccttgattat tccttttgta
    26521 gcctctttgg taagtagttt gattatgatt tatattattg gagcgcctat cgcagccttt
    26581 accaactggt tgacgagctt attacaaagc ttgggaagtg cttcaaatgg tttgatgggg
    26641 gcagttattg gagttctcag tgctgttgac tttggtggcc cacttaataa aacagtctat
    26701 gcgtttgtgt tgactttaca ggctgaaggt gtgaaagaac cattgactgc tttgcaattg
    26761 gtgaatactg ctacaccagt tggatttgga ttggcctatt ttatcgcgaa attactcaaa
    26821 aaaaatatct atactcaaga ggaaatcgaa acattgaaat cggctgttcc tatggggatt
    26881 gtcaatattg ttgaaggtgt aattccgatt gttatgaata acttggttcc aggtctcatt
    26941 gcaacaggta tcggtggtgc tgttggtggt gctgtttctt tgacaatggg tgctgattct
    27001 gccgtgccat ttggtggagt gcttatgtta ccaaccatga ctcgtccagt agctggtatt
    27061 tgtgctttgt tagccaacat tgtagtcaca ggacttgtct acgcgatttt gaaaaaacca
    27121 ataaaacata cagaaccagt tatgactgtt gaagaagaga ttgatttgtc agatattgaa
    27181 attttgtaag agggtaacga tgtcaagaat tgaattttca ccatctttga tgaccatgga
    27241 tttggacaaa ttcaaagagc agattacttt tttgaatgat aaagtagcat cttatcatat
    27301 cgatattatg gatggccatt ttgttcccaa tattaccttg tctccttggt tcattcaaga
    27361 agttcaaaaa attagtgaca cacctttatc agttcatctg atggtcacag acccaacctt
    27421 ttgggtagat caagttctcg atttacaatg tgagtatatt tgtattcatg ctgaagttct
    27481 gaatggtctt gcttttcgtt tgattgataa aattcatgat gcaggtctaa aggctggtgt
    27541 tgtccttaat cctgaaacac ctgtttctac aatctttccc tacattgatt tacttgacaa
    27601 agcaactatt atgactgtag atccaggttt tgcaggacaa cgctttttgg agtctacctt
    27661 gtataaaatc caagaactcc gtcagcttag agttcagaat ggttatcact acatcattga
    27721 gatggatggt tcttcgagtc gtaagacttt caaacaaatt gatgtggcag gaccagatat
    27781 ttatgttata ggtcgcagtg gattatttgg tttggatgac gatattgcca aagcctggga
    27841 tatctgttct agagattacg aagaaatgac cggaaaaaca atgccaatca aataatggtt
    27901 tgagaagaaa tttattagtt aggaggaata tatgtcacta caatcagtta acgccattcg
    27961 ttttcttggc gtagatgcta ttaacaaatc taattctggt cacccgggaa tcgtcatggg
    28021 tgctgcgcca atggcttata gcctatttac aaagcacctt agaattacac ctgagcagcc
    28081 aaactggatt aaccgagatc gctttatctt gtctgcgggt catggatcaa tgctactgta
    28141 tgctctcttg catttaacag ggtataacga tgtatccatg gacgagatta aaaatttccg
    28201 gcaatgggga tctaagacac ctggtcatcc tgaagtgacg catacgtctg gtgtggatgc
    28261 gacatctggt ccgcttggtc aggggatttc tactgccgtt ggtttcgccc aagcagagcg
    28321 ttttttagct gctaagtaca acaaagatgg ttttcctatt tttgaccatt atacttatgt
    28381 tatcgctgga gacggtgact tcatggaagg agtgtctgcg gaggcggctt cttatgcagg
    28441 tcatcaagct ttagataagc ttatcgtcct ctacgactcc aacgatatct gcttggatgg
    28501 tgagaccaaa gatactttct ctgaaaatgt tcgcgcccgt tacgatgctt atggttggca
    28561 tacagttctg gtagaagatg gaacagattt agcagcaatt tctacagcaa ttgagacggc
    28621 caaattttct ggtaagccga gtttgattga agtgaaaacg gtaattggtt acggctcacc
    28681 caataaaagt ggtacaaatg ctgttcatgg tgcaccacta ggagcagaag aaacaggagc
    28741 aactcgtaag tttttgggat gggattacga tccatttgaa gtaccagagg aagtatattc
    28801 tgatttcaag acaaatgtag cggatcgtgg tcaggaggca tacgatgctt gggctagttt
    28861 ggtgtctgat tacaaggttg cttatcccga agttgctagt gagattgacg ctattgtagc
    28921 tggaaaatcc cctgtaacca ttactgaaaa agacttccct gtctatgaga atggcttctc
    28981 tcaagcaact cgtaattcgt cccaagatgc tattaataca gcagcagcag ttttaccaac
    29041 cttcttaggt ggatcggcag acttagctca ctccaacatg acctacatca aggcagatgg
    29101 cttacaagat aaatataatc cattaaaccg caatattcag tttggggtac gtgaatttgc
    29161 catgggaaca atcctcaatg gaatggctct tcatggtggt ttacgagttt atggcggaac
    29221 cttctttgtt ttctctgact acgtcaaagc tgctattcgg ctatcagcca ttcaggagtt
    29281 gcctgtaact tatgtcttta cccatgattc aattgccgtt ggtgaagatg gtccaactca
    29341 tgaaccagtt gaacatttgg caggtttacg ctcaatgcca aacttgactg ttatccgtcc
    29401 agcggatgcc cgtgaaactc aagcggcttg gcatcatgcc ttgaccagta ccaccactcc
    29461 aactgtcatt gtcttaaccc gtcaaaactt ggtagttgaa gaagggacag actttggtaa
    29521 ggtcgctaaa ggagcctacg tcgtgtatga taccccggga tttgatacta ttatcattgc
    29581 tacaggatct gaggtcaatc tagctatcaa agctgctaag gaattggttt tacaaggtgg
    29641 taaagtacgt gtggtatcta tgccctcaac cgaactattt gatgctcaag atgctaccta
    29701 caaggaagac attttaccat ctaagactcg tcgtcgtgtg gccattgaaa tggcagcgac
    29761 ccaaagttgg tacaagtatg ttggtttgga tggcgcggtc atcggtattg acatcttcgg
    29821 tgcgtctgcc ccagctcaga ctgtgattga taattatgga tttacggtag agaatatcgt
    29881 tgctcaagtt aagtccctat agaaaccaat tacaatgaag atacagttgt tgtcagacta
    29941 gcagatgtag tgatagacac taatcagatg attggttatt taaaaactat aatgagaatg
    30001 taataattta tctacgaaag ttatagtaga tagtatacac aatagagtat accctgaaac
    30061 ggttgcgaag tacgctaatc actttgctac tgatctagat agtttcttta atcaataaac
    30121 acagcatcca cagattgact taggatattg taagtttttt gaaagctaga gagaaggtct
    30181 ctaaaattaa aaaacgcata gtataggatg ttaaaatgaa ctgaccccaa aagttagaca
    30241 gaaaaaatct aactttttgg ggtgttttat tatgaaatta agttatgatg ataaagttca
    30301 gatctatgaa cttagaaaac aaggatatag cttagagaag ctttcaaata aatttgggat
    30361 aaacaattct aatcttaggt acatgattaa attgattgat cgttacggaa tagagttcgt
    30421 caaaaaagga aaaaatcgtt actattctcc tgatttaaaa caagaaatga ttcataaagt
    30481 ctgacatgaa ggctggacta aagatagagt ttctcttgaa tacggcctcc caagtcgtac
    30541 gatacttctt aactggctag cacaatacag gaaaaacggg tatactattg ttgagaaaac
    30601 aagagagaga gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat
    30661 tgaaggagga aaaagagaaa gaagaaagac agaaattgtt caagaattaa tgactgagtt
    30721 ttcgttagat attcttctaa aagccattaa actagctcgt tggacctact actatcactt
    30781 gaaacagcta gacaaaccag ata
//
LOCUS       DAVGJJ010000014        29045 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000014 DAVGJJ010000000
VERSION     DAVGJJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.014"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            68..3169
                     /locus_tag="VVE35_000595"
     CDS             68..3169
                     /locus_tag="VVE35_000595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU3060837.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDISLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDVSAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGLSKDLALWIIEHRPYSNIEDFLAKLPENYLKHPLLEPLVKV
                     GLFDLFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSEQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3251..4258
                     /gene="pfkA"
                     /locus_tag="VVE35_000596"
     CDS             3251..4258
                     /gene="pfkA"
                     /locus_tag="VVE35_000596"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU3060838.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4317..5822
                     /gene="pyk"
                     /locus_tag="VVE35_000597"
     CDS             4317..5822
                     /gene="pyk"
                     /locus_tag="VVE35_000597"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU3060839.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6485..6707)
                     /locus_tag="VVE35_000598"
                     /pseudo
     CDS             complement(6485..6707)
                     /locus_tag="VVE35_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7555..8427
                     /locus_tag="VVE35_000599"
     CDS             7555..8427
                     /locus_tag="VVE35_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU3060840.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8576..8917)
                     /locus_tag="VVE35_000600"
                     /pseudo
     CDS             complement(8576..8917)
                     /locus_tag="VVE35_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            9060..9371
                     /locus_tag="VVE35_000601"
     CDS             9060..9371
                     /locus_tag="VVE35_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060841.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9375..10073
                     /locus_tag="VVE35_000602"
     CDS             9375..10073
                     /locus_tag="VVE35_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3060842.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLVGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALFDTIFLENFGHELYTQAPK
                     EIASLMNEWLAYFL"
     gene            complement(10202..11017)
                     /locus_tag="VVE35_000603"
     CDS             complement(10202..11017)
                     /locus_tag="VVE35_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU3060843.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11014..11919)
                     /locus_tag="VVE35_000604"
     CDS             complement(11014..11919)
                     /locus_tag="VVE35_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU3060844.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            12305..14434
                     /locus_tag="VVE35_000605"
     CDS             12305..14434
                     /locus_tag="VVE35_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU3060845.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEVQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            14421..14870
                     /locus_tag="VVE35_000606"
     CDS             14421..14870
                     /locus_tag="VVE35_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU3060846.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            14929..15201
                     /locus_tag="VVE35_000607"
     CDS             14929..15201
                     /locus_tag="VVE35_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU3060847.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            16172..16930
                     /locus_tag="VVE35_000608"
     CDS             16172..16930
                     /locus_tag="VVE35_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060848.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            16932..18920
                     /locus_tag="VVE35_000609"
     CDS             16932..18920
                     /locus_tag="VVE35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3060849.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(18962..19663)
                     /locus_tag="VVE35_000610"
     CDS             complement(18962..19663)
                     /locus_tag="VVE35_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU3060850.1"
                     /translation="MFVNETNHEIDSNFAGRLNILRAGVLGANDGIISIAGVVIGVAS
                     ATTNIWIIFLSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKK
                     SLYAAYIQNGECKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAF
                     FLRSLPPMLSVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGL
                     LTMGVTFLLEQLFSI"
     gene            20220..21695
                     /locus_tag="VVE35_000611"
     CDS             20220..21695
                     /locus_tag="VVE35_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU3060851.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(21661..22377)
                     /locus_tag="VVE35_000612"
     CDS             complement(21661..22377)
                     /locus_tag="VVE35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU3060852.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTCNPLLIKEFAKSLSLRKTKTNKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILTRHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASNSRAFCRALKSSPNHQ
                     IEIRNSLDKTIDFSYSLSFS"
     gene            22633..23493
                     /gene="speE"
                     /locus_tag="VVE35_000613"
     CDS             22633..23493
                     /gene="speE"
                     /locus_tag="VVE35_000613"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU3060853.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            23490..24749
                     /locus_tag="VVE35_000614"
     CDS             23490..24749
                     /locus_tag="VVE35_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU3060854.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            24749..25876
                     /gene="nspC"
                     /locus_tag="VVE35_000615"
     CDS             24749..25876
                     /gene="nspC"
                     /locus_tag="VVE35_000615"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU3060855.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEITRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            25873..26958
                     /gene="aguA"
                     /locus_tag="VVE35_000616"
     CDS             25873..26958
                     /gene="aguA"
                     /locus_tag="VVE35_000616"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU3060856.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEVLEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGSAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            26968..27843
                     /gene="aguB"
                     /locus_tag="VVE35_000617"
     CDS             26968..27843
                     /gene="aguB"
                     /locus_tag="VVE35_000617"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU3060857.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(28024..28833)
                     /locus_tag="VVE35_000618"
     CDS             complement(28024..28833)
                     /locus_tag="VVE35_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3060858.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASNYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         8769 a         5390 c         6282 g         8604 t
ORIGIN      
        1 ctcaagagaa gccatttata ggtgaaaatg gtataatata gtgagaagga tagaggagaa
       61 gtgtaaattg atcgcacaac tagatacaaa aacagtctat agttttatgg aaagcgtcat
      121 ttcgatcgaa aagtatgtga gagcagctaa agaatacggc tacactcatt tggctatgat
      181 ggatattgac aatctttatg gcgctttcga ctttctagag attacaaaaa aatacggcat
      241 tcatcctttg ctagggcttg aaatgacagt gtttgtagat gatcaggaag tgaatttgcg
      301 ctttttagct ctatctagtg tgggctatca gcagttgatg aagctttcga cagccaagat
      361 gcagggggag aaaacttggt cagtcctgtc ccagtacctg gaggatatcg cggtcattgt
      421 gccttatttt gatagagttg agtcgttaga actaggctgt gattactata taggggttta
      481 tccagaaaca ctagcaagcg aatttcatca tcctatctta cctctttatc gggtcaacgc
      541 ttttgaaagc agggatagag aagttcttca agttttaaca gcgattaaag aaaatctacc
      601 gctcagagaa gttcccttgc gttcgagaca agatgtcttt atatcagcaa gttctttaga
      661 gaaactattc caagagcgtt ttccgcaagc tttggacaat ttagaaaagc ttatttcagg
      721 catttcttac gacttggata ttagtctgaa actgcctcgt tttaatccag ctagaccagc
      781 agtagaggag ttgagagagc gtgctgaact ggggcttgtt cagaaggggt tgactagtaa
      841 agaatatcaa gatagactag accaagaatt gtctgttatt catgatatgg gctttgatga
      901 ttatttcttg gttgtttggg atttgttgcg ttttggacga tcgaatggct attatatggg
      961 aatgggaagg ggttctgcag taggcagttt ggtttcttat gccttagaca tcacggggat
     1021 tgacccagta gagaaaaatc tgatttttga acgctttctt aatcgtgaac gctataccat
     1081 gcctgatatt gatattgata tcccagatat ttatcgtcca gattttatca gatatgttgg
     1141 taataaatat ggtagtaaac atgcggcaca aatcgttact ttttcaacct ttggagccaa
     1201 gcaagctctt cgagatgttt tgaaacgctt tggtgtgcca gagtatgaat tatctgcaat
     1261 tactaagaaa atcagttttc gtgacaatct taagtcggcc tatgagggca atctccagtt
     1321 tcgtcagcaa atcaatagta agttagaata ccaaaaagct tttgagattg cttgcaagat
     1381 agagggctat ccaaggcaaa cctctgtcca tgcggctggt gttgtaatta gtgaccaaga
     1441 tttaaccaac tacattcctc taaagtatgg tgatgaaatt ccactgactc agtatgatgc
     1501 tcatggagtt gaggctagcg gacttttgaa gatggacttt ctgggactac gaaatttgac
     1561 ctttgtccag aagatgcaag agttgcttgc tgaaacagaa ggtattcatc tgaaaattga
     1621 agaaatcgat ttagaagaca aagaaacgtt agctttattt gcctctggta atacaaaagg
     1681 tatctttcaa tttgagcaac caggtgccat tcgtctgctt aagcgtgtgc aaccagtctg
     1741 ttttgaagat gtcgtcgcga ctacttctct aaatcgaccg ggtgctagtg actatatcaa
     1801 taattttgtg gcaagaaagc atgggcagga agaagtgact gttctggatc cagtactgga
     1861 ggatattttg gctccaacct acggcataat gctctatcag gagcaggtta tgcaggttgc
     1921 ccagcgattt gccggattta gtcttgggaa agccgatatt ttgcgtcggg ctatggggaa
     1981 aaaggatgtc tctgccatgc atgagatgag ggcttccttt attcaaggtt cattagaagc
     2041 tggtcatact gtggaaaaag cagagcaggt ctttgatgtt atggagaagt ttgcaggcta
     2101 tggttttaac aggtcacacg cctatgccta ctcagcattg gccttccagt tggcttattt
     2161 caaaacgcat tatccagcca ttttttatca ggtcatgtta aattcttcca acagtgatta
     2221 cttaatagat gcacttgaag caggttttga agtagcctct ctgtccatca acacgattcc
     2281 ctatcacgat aaaattgcca acaaggccat ctatctaggt ttgaaatcga ttaaggggct
     2341 cagcaaggac ttggcgcttt ggattattga acatagacct tattctaata ttgaagattt
     2401 tttagctaaa ctacctgaga attatctgaa acatcctctg ctagaacctt tggtaaaagt
     2461 tggccttttt gatttatttg aaaaaaatcg tcaaaaagta tttaataact tagctaatct
     2521 atttgaattt gtgaaagagt tgggaagttt gtttggagat gctatttata gttggcagga
     2581 atcggaagat tggacggaac aagaaaaatt ttatatggaa caagagcttt tagggatagg
     2641 tgtcagcaaa catccactac aagctattgc aagtaaggct atttacccga ttaccccaat
     2701 cggaaatttg tcagaaaata gctatgctat tattttggtt gaagttcaga aaataaaagt
     2761 gattcgtacc aaaaagggtg aaaatatggc cttcttacag gcagatgata gtaagaaaaa
     2821 attggatgtc actctctttt cagacttata tcgtcaggtt ggacaggaaa taaaagaggg
     2881 agccttctac tatgtaaaag gaaaaataca atcacgtgat ggccgtctgc aaatgattgc
     2941 acaagaaata agagaagcag ttgctgaacg cttttggatt caggttaaaa atcatgactc
     3001 ggagcaagag atgtcacgta ttttagacca gtataaaggc ccaatcccag tcatcatccg
     3061 gtatgaagag gaacagaaaa ccatcgtttc tccccatcat tttgtagcta aatctgatga
     3121 attagaggcg aaattgaatg gaatagttat gaaaacgatt tatcgctaaa attacggaaa
     3181 atagaagaat tttcaacgta aatgtggtat aatcagtaag aatgttaaaa gaaaaaggag
     3241 cataaccaat atgaaacgta ttgctgtttt gactagtggt ggagacgccc ctggtatgaa
     3301 cgctgccatc cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tttttggtat
     3361 ctatgacgga tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt
     3421 aggggacatc atttctcgtg gtggtacttt ccttcactca gctcgttacc cagagttcgc
     3481 tcaacttgaa gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt
     3541 agttgttatc ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt
     3601 cccagctatt ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat
     3661 cggttttgac acagcggtta ctactgccat ggacgctatc gataagattc gtgatacatc
     3721 atcaagtcac cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc
     3781 tctttgggct ggtattgcaa ctggtgctga tgaaatcatc atccctgaag caggcttcaa
     3841 gatggaagat atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat
     3901 catcgtctta gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc
     3961 tggagataca agcgaccttc gtgtaacaga acttggacat attcaacgtg gtggttctcc
     4021 aactgcgcgt gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa
     4081 agaaggtatc ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat
     4141 tcttggtact gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt
     4201 taacaaccca cacaaagccg atattgagct atctagcttg aataagagct tgtcataatt
     4261 tacaatttag ttaataagac caaaaaggtc atatatataa aggagtcaca aaaatcatga
     4321 acaaacgtgt aaaaatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa
     4381 aattcggtga ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca
     4441 ttgctaaatt gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc
     4501 accaagaaca aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa
     4561 aagttggttt ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg
     4621 aagctaaaga atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa
     4681 tcaaatcaac tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg
     4741 atgttgaagt tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg
     4801 ctaaagatga tgaaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta
     4861 aacaaaaagg tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg
     4921 ataacgacga tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg
     4981 tacgtactgc aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac
     5041 atgttcaatt gttcgctaaa atcgaaaacc aacaaggtat cgataactta gacgaaatca
     5101 tcgaagcagc tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg
     5161 aaatggttcc agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg
     5221 ttatcactgc aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag
     5281 aagtatcaga cgtattcaac gctgttatcg acggaactga cgctacaatg ctgtcaggcg
     5341 agtctgcaaa cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga
     5401 acgctcaagc tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact
     5461 ctaagacaga agtaatggct tctgctgtta aagatgctac tagctcaatg gatatcaaat
     5521 tggttgtaac tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa
     5581 atgctgacat cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact
     5641 ggggtgttat cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg
     5701 ctgaacgtaa agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg
     5761 ctggtgtgcc agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt
     5821 aagaaaaata taaaaaccta tcatatccag ctttctagcc tgtatgatgg gctctttttt
     5881 tgtccagggg gcaaaacttt cataatggat tgatactctt cgaaaatctc ttcaaaccac
     5941 gtcagcgtcg ccttaccgta ctcaagtaca gcttgcggct agcttcctag tttgctcttt
     6001 gattttcatt gagtatgaaa taagatatgc acaaattgat tagaaagtca aatgaatttc
     6061 tacaaatgtt ttagcaatcg taatgtactt gtctagattc gatctgatat attttcgatt
     6121 taatgatatg gtatttaaaa ccttcaaagt agcttgctcc attcttttac ttacgtgagt
     6181 gtagatgtta tttactgttt tagcgttttt gtgttccact ctaaccatta tagcattctt
     6241 ctcagctagt gtactaagga gtgtgtgcct gaaaatatgg gaactaaggg gctggtttat
     6301 cggtttctct agtttagtat ttgccttttg caaagtgatg ttaaatgcct ttctctaaat
     6361 ttacatatca ctattgttta acaaaatcta atctatttta ggtcacttat tctttttttg
     6421 aaatgtagaa tgaacttttt caaagttttt cgaatctttt aaaatctgtt tgttttatat
     6481 cgccattctc cctatatagc ctgacaactt tcccgatggt acgaatatgg ttgctttcgt
     6541 ctaggtggat gtcggggtat ttgggattga gtttttttaa ggtagccttg gcggagtttc
     6601 ttgacatagt tagcgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg
     6661 gtattcttga taaataggta gtcgctgttt cttatctttg cctccatgga cttgctgacg
     6721 acataagcga ttgggtcgta gtcgtctggg ataatggaaa ctccatatct aaatcgttgt
     6781 cctgcatcga gcggctacct gcagagataa actacctaac acgagagtaa gtagtctgtc
     6841 tgtagtcgtc cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct
     6901 ctcggcatag gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg
     6961 gatatcgcta ggagaatcct tttgaactgg agaaaagagg gcatcaatca agctactgaa
     7021 tactttaact aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt
     7081 tccaatggtg tttttactta taccaatctt agtacccaat tcttattgag tccaaccatt
     7141 actagtctat attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa
     7201 catttctagg aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctac
     7261 tatatttttc gcctctaaca atcgcaatct cttctttatc tacgaatgaa atcgctttct
     7321 atttttgtaa gtaaagcatc acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc
     7381 gtaaaaaaat tgttgacaga gcacgaaacg cctactataa ttaaatcaag cttaaggaaa
     7441 taacaaaaaa acaaatcgga gggaaagacg atgaacatat taaatgagaa agtaatcaat
     7501 atctgtaaag cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
     7561 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
     7621 tgtcaacaac aacatgctac ttctgagggg acgaatcaaa ggcaaagcag ttcagcgaaa
     7681 gttccatgga aagcttcata caccaaccta aacaaccaag taagtacaga agaggtcaaa
     7741 tctctcttat cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat
     7801 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
     7861 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgattttgtt
     7921 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
     7981 cttgaaaaga atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc
     8041 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgaggca
     8101 actacagatg tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc
     8161 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atctggatgg cgagtatctg
     8221 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     8281 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     8341 tatttggaca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     8401 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     8461 ttatttctat ttttgatgag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     8521 aaaaatgaaa catttattgg ctatgtccaa agatacgata ttgatgggaa tggctatcaa
     8581 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaaaacca ctttgaacaa
     8641 gagttacttc aacccattgg ctccgacgga ttaagttgct ttcgtgaata ccaaaatcag
     8701 ccgcaatttc ttcataagtg cggtattctc gcacatattg aagagtggcc ataagaaggt
     8761 cttctaggct taatttaggt tttcgtccac cttttgcgtg tttaagttga taagctgttt
     8821 ttaatacagc taacatctct tcaaaagtcg tgcgctgaac accaacaaga cgcttaaatc
     8881 gtgcatcagt tagttgttta cttgcttcat cattcataga actattatac catgttttgt
     8941 ttcgcaggaa atctaatgct aaaatcttat cagtgagaag atttcttaaa gtttattctt
     9001 tgtagtttca tctgtattag tacataattt aacttagaga aaataggagg actaatcgaa
     9061 tggaattaaa agattttaca gaaaaagaac aggaaatgat taagaaaagg cttacaatgt
     9121 ctaatattag tgacaaagaa actactgaga agattctggc gctagtacca caagacttga
     9181 ttaagcgaat cccgtttttt gtcagaaaac atgctacaac acgtacgatt aaacgcattt
     9241 caattgaaca ccctgaactc tacgctgtag ctcaaacaag tggtgagatt ccagaaaaag
     9301 aacgcgaaga attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata
     9361 gtattaagta gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata
     9421 gcaaggattt tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc
     9481 atcttcgaga tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat
     9541 ctatctgtct gataggccat tctcttgtag gagatttagc tcgttatctc gcatcggaat
     9601 ttgaagaagt aaaaaaactg attcttttgg atggtggcta tctagattta gataagattt
     9661 tacctttgga tacagagtta gaggaaacta aaaattatat caaatctcaa attgttttgg
     9721 acttagctct tcttatttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg
     9781 agaaagctgt aagacagtct tatcactgga atgttgagta taatagatat gagttggcta
     9841 taaattatga aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga
     9901 gagaagtggg agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag
     9961 aggaagccct atttgatact atttttttag aaaactttgg ccatgagctt tatactcaag
    10021 cacctaaaga aatcgctagt ctaatgaatg agtggctcgc ttattttcta tgagctactt
    10081 gtattgctgg aaatggtata agatgaaaat ctacaagtca ctaattttca tataacaaaa
    10141 aacgaacagg ctctttttct gttattcaga agagaacttg ttcgttttta tcttgatttt
    10201 cttaaaaagc tctatataca tagggatttt ctggtttatc gactttggta aagctgtcga
    10261 tttccaaggt tggaaggttt tgtttgagtt ctttatggta gtgattgacc agttttcctt
    10321 tggctgttat ccaagtgttg ttttcatact gccgggtatt gcccttggtc agcaatccat
    10381 agacaccaga atctgcgata cagtggataa tgccgaagcg gaacagaaat tggctattgg
    10441 catgactggg gtcgttatag acaaagcctg taaactggat tgtcttgccc tcaaactcat
    10501 ctggatagtc gtagatagcc tccatgactt ccatatagtt ttcattagtg atctgaatac
    10561 tatcttggga taagtattta tccgccgccg ttcgcatttc cttttcatag gctgattttg
    10621 aaaaataaga actggtatct ggtttcaaat attggcttgt cgtcccttcg cttgtctgaa
    10681 tggctagatc cgttccttct gataggggga aatgataacc tttagcagaa acggtctgag
    10741 aatccaagct aacagttggg aaagttaagc cgatgacaag tggaatagcc agaagagaaa
    10801 tactcgtcat cttggctaat cggctgttca gatgactgtg ggttttgact tgcttcatcc
    10861 agatatacaa ttgaacaata gccaagataa aggaaagcac catggaaata taggccagat
    10921 aggaatagtg catgttgatg tactggttta gtttgcccga cagatggagg taaatagtca
    10981 gttcaaaata gccagctaaa actaaaaatc gaatcatagg atgactccaa tcaagagaga
    11041 ataggctaaa acgaccagag tcacaatcgt aatgaagtga ctaataaatc gtgctttcaa
    11101 gtaatttttc atcatgagaa tatttttgat atccagcatt gggccaatga cgagaaaggc
    11161 cagaactgga gccaaaccga aactcgagag tagagaagca cctataaagg catccgcctc
    11221 actacagagc gaaagaagaa aggccaaaag catcaagagc aggatggcaa aaagaggagt
    11281 cgcactgata gaggtcagaa tccgagtcgg aacgtagacc tgtattatag aagcaaagag
    11341 gcagccaaat accaaataac gccccgtatc aaaaaattca tcaatggcct gcacaaagac
    11401 ttgaaaaact ttttttgcag aactcaagta agaaaaatca tgctcatgac aagccagacg
    11461 attttctttc tgaatcggtt cttgccagaa aaatcctaga aaaattccta gtattacagc
    11521 cacaagaatg gaacccagag ctcgtaatag ggcgacatgg aaggagttgc caaaggcaga
    11581 ataggtcgca aaaagaacaa tgggattgat aacaggtgct gtcacaagaa aaggaacggc
    11641 cgtgtaactt ggaacctttt tttccagaaa acgattgatg atggggacga ttccacattc
    11701 acaagaaggg aaaaacatac cgacgaaggt cccaaaaaag attctccccc aacgatttcg
    11761 agggagaaaa tgataaacct tgtcaggtgt gatataaact tcaatcagcc ctgagacaat
    11821 gcttcctatc agaacgaagg gaagggcttc aatgataatg gagagaaaaa tggctccagt
    11881 ttgaagtaca ctagatggga gttgttgaaa agcagtcatc tagtttttct tttctgcttt
    11941 ggcttttttc tctttgtcat cagggaaagt aacttgtttc aaatcaggga gtttagcaaa
    12001 ctcttcaaac atcttgtcca agtcgtctcg ttttgtaaat ttgacagcca taggtgtacc
    12061 tcgattctaa attctacctc tattatacta ttattttgcg gaaaagggag ttttttaatt
    12121 tgttttcgat agttgatact cttcaaacca cgtcagcttc gccttaccgt atatatgtga
    12181 cttatccaca acctcaaaac agtattttga gcagcctgcg gctagcttcc tagtttgctt
    12241 tttgattttc attgagtatg aaaaggattg gagaaagcct tccagcttgt ggtataatag
    12301 atttatggat aaaaaatatg aaaaaatctc tcaggatttg ggagtgacgt taaagcaaat
    12361 tgataccgtt ctaagtttga cagctgaagg ggcgactatt ccctttatcg cgcgttatcg
    12421 caaggacatg actggtagtc tggatgaggt ggcgattaag gctattattg atttggataa
    12481 aagtctgaca aatctcaatg accgtaagga agctgtctta gctaagattc aagaacaagg
    12541 taagttgacc aaggaattgg aagaagctat cttagttgcc gaaaaattag cagacgttga
    12601 agaactctat cttccttata aggaaaagcg tcgtaccaag gcaaccattg cccgtgaagc
    12661 tggactcttt cctcttgctc gtttgatttt gcagaatata gttgacttag agaaagaagc
    12721 tgaaaagttc gtctgtgaag gatttgcgac tggcaaggaa gccttgaccg gtgcagttga
    12781 tattttggtc gaagccttat cggaagatgt gaccttgcgt tctatgactt atcaggaagt
    12841 gctgagacac tctaaactta cttctcaagc caaggatgaa agtcttgatg aaaagcaggt
    12901 ttttcagatt tattatgatt tttcagagac agttggaact atgcaaggct atcgtacctt
    12961 ggctctcaat cgtggggaga aacttggtgt cttgaagatc ggttttgaac atgcgacgga
    13021 ccgtattctt gccttctttg ctactcgttt caaggtgaaa aatgcttata ttgatgaagt
    13081 tgttcagcaa tccgttaaga aaaaggtctt gcctgctatc gagcgtcgta ttcggacaga
    13141 attaactgag aaagctgaag aaggagctat ccaacttttt tctgacaatc tgcgcaatct
    13201 cctcttggtt gctccactga aagggcgcgt ggttcttgga tttgacccag cctttcgtac
    13261 aggtgccaag ttagctgtcg tggatgcaac aggaaaaatg ctgacaactc aggttattta
    13321 tcctgttaaa ccagcatcag ctcgtcaaat cgaagaagcc aagaaagatt tagcagattt
    13381 aattggtcaa tacggtgtag agattattgc cattggaaat ggaacggcca gtcgtgaaag
    13441 tgaagctttt gtagcggaag ttctgaaaga tttccctgaa gtcagctatg ttatcgttaa
    13501 tgaaagtggt gcttctgtct attctgccag cgaacttgct cgtcaggagt ttccagactt
    13561 gaccgttgaa aaacgctctg ccatttctat cgcccgtcgt ttgcaagatc ctcttgcgga
    13621 attggtcaaa atcgatccta agtcaattgg tgtcggtcaa taccaacacg atgtcagtca
    13681 gaagaaacta tctgagagtc tggactttgt tgtcgataca gtggttaacc aagttggtgt
    13741 caatgtcaat acagctagcc cagctcttct ttcacacgta gctggactca acaaaactat
    13801 ctctgaaaat attgtcaaat accgcgagga agaaggaaaa atcacttcac gcgcccaaat
    13861 caagaaagtt cctcgtctgg gagccaaggc ctttgagcag gctgctggtt tccttcgtat
    13921 ccctgaaagt agcaatatcc ttgataatac aggagttcac ccagagaatt acactgcagt
    13981 taaggaactc ttcaaacgtt tagatattaa agacctaaac gaagaagtcc aaagcaaact
    14041 caagtccctt tcagtcaagg aaatggctca agagctagac ctcggtccag aaacccttaa
    14101 agatatcatt gcagatcttc tcaaaccagg tcgagatttc cgtgattcct ttgacgcacc
    14161 tgtgcttcgc caagatgtcc tagatatcaa agacctagtg gtaggtcaga agctagaagg
    14221 tgttgtgcgt aacgttgttg acttcggtgc cttcgttgat atcgggattc acgaggacgg
    14281 cttgattcat atttctcata tgagtcgcaa gtttatcaaa caccctagtc aagtggtgtc
    14341 agttggagat ttggtgaccg tttgggtcaa gaaaattgat actgaacgtg aaaaagttaa
    14401 tctgtcgctc ctcgctccaa atgaaactga ctgattatgt caagcaggtt tcactagaag
    14461 acttcgggag agcttttatc catcatgtcc agtggaatag gcgtctacgt tcgacaggtg
    14521 ggcgattttt ccccaaagat gggcatctgg attttaatcc caaggtttat cagaaactgg
    14581 gtatggaggt ttttaggaaa attgtccgac atgaactctg tcattatcac ctttatttcc
    14641 aagggaaggg ctatcaacac aaggatcggg attttaagga acttttgaaa gcagtggatg
    14701 gattaaggtt tgtaccatcc ttgcccaata gtaactctaa accactcaag ctttatcgtt
    14761 gtcaatcctg ccagcaaagg tatcagcgca agcgtaggat tgataccaaa cgctatcgct
    14821 gtggactttg tcgaggtaaa ttgcttctga taaatcagcc tgaggactga tgaaagccga
    14881 gcctccccgt gatatactat gtctaaccta gcagaaagag gaaatatcat gaacagtaaa
    14941 ttttataaaa tgagacgcaa tcgcatgatt tcaggagttc tggcaggtct gtcagacaag
    15001 tggaattttg atgttaccct agttcgtttc ttgtttgcca tttttacagt tgcaaacttt
    15061 ggacttggcg tgattatcta tatcatccta gcttctatca tgcctaccaa ggaagaaatc
    15121 gaagcagaaa tgtacggaac aggcccacgt aaacgcaaag aagcccaagc cattgacgat
    15181 aatgaaggct ggttttggtg aggttttaca aaagatttgg aatacgcaaa aagctttaaa
    15241 tcaatagtct aaactgcttg atttaaagct tttttacttt tatactcaat aaaaatcaaa
    15301 gtgcaaacta ggaagccagc cgcaggctgt aatttagtcg gcaatgtgaa gttgatatag
    15361 tttgcacttt gatttttgaa tagtattaat aatatttcgc cagcttcatc cacaaactat
    15421 agtggattgg aactagaata gtacacctct acttctaaaa aattgttaga aatcgatttg
    15481 attgtcctga tcaatttgtc ctgttcttat ttcattttac tataaaagca gtaatttaaa
    15541 cgcagttttt taatctattt ttgctgctga gacaatacat ttttggaaaa atggtggatg
    15601 ctgataacaa ctgataacca gcaaataaca taagcagtgc cggtaaattc tgtaccgata
    15661 aagtgtgtgt tcaactgaga gctgaccgat gaatggagag agcagataga gaaatccgat
    15721 gagtatatgt tctttatgtt tcattaaagc gcaccttctt tccttatagg ttgaacgact
    15781 tgcaagtgtt aaattgaatg gcatgttatc taggtaacct tactaaaatg ttagttcccg
    15841 cttcctcctt tttaaagtaa gccttttcgt acttatattt tacaacttta aaaataggaa
    15901 atcaaatcat tttctaagta attttgctta tatttccccc aatcttacaa aaatgtaaga
    15961 ttaaagtctc ttttgtaagg ttaggttatg gcaagcggtc tttttttgat gtaaactaga
    16021 ctcataaaga acaaaggagt aacatcatga aacaaatctt acataagaaa actagaaaac
    16081 caagtcacaa agaaattgag cgcattcagc tgggatgcac tatgatgcaa gctacatttc
    16141 atttgatggg atattaagaa aggagattct catgacactt ttagatgtaa aacacgttca
    16201 aaaaatttat aaaactcgtt ttcagggcaa ccaagtagag gccctcaagg atattcactt
    16261 caccgtagaa aagggtgact acgttgctat catgggtgag tctggttctg gtaaatccac
    16321 tctgctcaat atcctagcta tgctggataa accaagtcgt gggcaggttt acctaaacgg
    16381 aactgataca gcaactatta aaaattcaca ggcttctagt ttccggcgtg aaaagctagg
    16441 atttgtcttc caagacttta acttgctaga tactctgtct gttaaggaca atatcttgct
    16501 tccgcttgtc ttgtcaagaa gacctataac ggagatgatg aagaaattgg tgatgacagc
    16561 tgaaaatctg ggtatcaacc aattgcaaga gaagtaccct tacgagattt ctggtggtca
    16621 gaaacagcgt gtagcagtag cccgcgccat catcacagaa cctgaaattc tccttgcgga
    16681 cgagccaaca ggagcccttg attccaagtc atctgcagcc ttacttgatg tctttgatga
    16741 aatcaatgag cgtgggcaaa ccatcctcat ggtaacccac tcaacagcag ctgctagcag
    16801 ggccaagcgt gttctcttta tcaaagacgg cattctttac aatcaaatct accgtggaga
    16861 gaagacagaa cgtcagatgt tccaagaaat ctctgatacc ttgactgtca tggcaagcga
    16921 ggtgaattag tatgtttcga ttaaccaata agttagcggt atcgaacttg attaaaaacc
    16981 gcaaactcta ctatcctttt gcgctggctg ttctcttggc agtcactgtc acctatctct
    17041 tttactccct aaccttcaat cccaagattg ctgaaatccg tggaggaaca accattcagg
    17101 ctacacttgg atttggtatg tttgtcgtca cccttgcgtc agccattatc gttctctatg
    17161 ccaatagttt tgtcatgaag aaccgttcca aggaactagg aatttatggc atgttgggct
    17221 tggagaagcg tcatcttatc agtatgacct ttaaggagtt agtggtattt gggattctaa
    17281 ctgttggagc gggtatcggt attggagcct tgtttgacaa gttaattttc gctttcctgc
    17341 tcaaactaat gaaattgaag gttgagctgg ttgctacctt ccaaatgaat gttgtcattg
    17401 cagtacttct tgtctttgga ttgattttcc taggcctcat gttcctgaat gcccttcgaa
    17461 tcgcccgtat gaatgccctc cagctctcgc gtgagaaagc aagcggagag aaaagaggtc
    17521 gtttcctacc tctccaaacg attcttggtt ccataagttt agggattggc tattatcttg
    17581 cccttacggt aaccgatcct cttacagccc taacaacttt cttcctagct gttttgctgg
    17641 ttatctttgg tacttatcta ttgtttaatg cagggattac agtcttccta caaatcttaa
    17701 agaaaaacaa gaaatactat taccaaccta ataacctcat ctctgtttcc aacttgattt
    17761 tccgtatgaa gaaaaatgcg gttggactag caaccatcgc tattttgtca acaatggttt
    17821 tggtaaccat gtcagcagcg acaagcattt tcaattccgc agaaagcttt aaaaaagttc
    17881 taaatcctca tgattttggg gtttcagggc aaaatgttga aaaagaagat ttggacaaac
    17941 tcttgagcca gtttgcaagt gacaaaggtt atagtgtcaa agagaaagaa gtacttcgtt
    18001 acagtaactt tggtattgca aatcaagaag gaaccaagtt aactattttt gaaaaaggac
    18061 aaaaccgtgt ccaacccaaa acagttttca tggtatttga ccaaaaagat tatgaaaata
    18121 tgactggtca aaaactgtct ctatcaggaa atgaggtcgg tctctttgcc aaaaatgacg
    18181 gactgaaagg acagaaagct ctaactctaa atgatcatca attttctgtc aaagaagaat
    18241 ttaataaaga tttcattgtg aaccatgttc caaataagtt taatatcttg actactgatt
    18301 acaattacct tgttgttcct gatttacaag cctttttgga tcaattccca gattcggata
    18361 tctataatca gttttacggt ggtatgaatg taagtatcag tgaagaagag caactcaagg
    18421 tcgctgaaga atatgaaaaa tacttacaaa agtttaatgc tcaattaaac actgaaggta
    18481 actatgtgta tggtagcaat ctagcagatg ctagttctca gatgagtgcc ctctttggtg
    18541 gtgtcttctt tatcggtatt ttcctatcca ttatctttat ggtcggaact gttctggtca
    18601 tctactacaa acaaatttct gaaggctacg aagaccgtga acgctttatt atcttgcaga
    18661 aagtcggttt ggaccaaaag caaatcaagc aaaccatcaa caaacaggtt ttaactgttt
    18721 tcttccttcc tttgctcttt gccttcatac atctcgcctt tgcctaccat atgcttagcc
    18781 tgattttaaa agtgattggt gtactggata cgactatgat gttgattgtg accttgtcta
    18841 tctgcgctat cttcctcatc gcctatgtgc tgattttcat gattacttca agaagttatc
    18901 gcaagattgt gcaaatgtaa aaaagatacc tcgacttcaa aatcgaggta tttcttgtat
    18961 tctaaatgct gaaaagttgt tcgagcagga aggtaactcc catggtcaag agaccaatag
    19021 caaggttccg aatcatagct gttctagtcg gatcctttcc aagtctggca ctagtgtaac
    19081 cagtgagaag aagggccaca ccgacaataa ggacagtagc agggatgcga tattcacttg
    19141 ggaatatggt cactgacagc attggaggca aacttctaag gaaaaaggca acgaagctag
    19201 aaatggcagc gtgccaagga ttggtaaatt cttcatactc aatcccatat ttttcctcta
    19261 ccagagcctt gagtggattt ttaagaaaga tcttattggt caagagttgg gcagaagttt
    19321 tgcattctcc attttggata taagcagcat agagggattt tttggctagt tccctatctt
    19381 ggtctaacaa gagtttttct cgcgaaacgg cagcttcctc ggtatctttt ggagttgaaa
    19441 cggatacata ttctccacca gccattgaaa aggcaccagc taagatagcc gcaaatcctg
    19501 ataaaaagat aatccagata ttggtcgtgg cactggcaac cccgataacc acaccagcaa
    19561 tggaaataat tccatcgtta gcaccaagaa cacctgcgcg caggatattt aaacgacctg
    19621 caaaatttga atcgatttcg tgatttgttt catttacgaa catatagtaa aatgaaacaa
    19681 gaacagaaca aatcgatcag gacagtaaaa tctatttcta acaatgtttt agaagcagag
    19741 gtgtactatt ctagtttcaa tctactatat ttttattttt tatcaaaaaa tactttacaa
    19801 gttcttaaaa acatgatata gtaataaagc ttagaaaatg agatgatgtt ttctagcaaa
    19861 tataaacccg agtaaaaaat gcctacggac aggcagggtt gaatgccgaa gcgtggttga
    19921 aaagccacat tattgatagg gttaaaagcc tacttttata agttgatgtt aggacacttg
    19981 ttctaattca taaattttta gtgtggtgaa agcacacgtc atcttgtgaa acgatcaata
    20041 aagtacgtaa tatttgctac tagagagtta ggaaacatcg ggaacagaca tactcaacag
    20101 aaaccaaaat aaacacgtca gaagattgca gagcaggtga aaacctgctc ttttttcatg
    20161 agtcaacctt tagttcctta gttttcataa ggtcctaaaa atattgaaag gagtatgttt
    20221 tgaaagagtt agatcaaaac caagccccaa tttatgaggc cttggtgaag ttacgcaaga
    20281 aaaggattgt tccctttgat gttccaggtc acaagcgtgg acggggaaat ccagaacttg
    20341 tcgaactctt aggagaaaaa tgtgtaggca ttgatgtcaa ttcgatgaaa cccttggata
    20401 atttaggcca tcctatttcg attattcgtg atgcagagga gctggctgca gatgcttttg
    20461 gagctagcca tgcctttcta atgattggtg gaacaacttc atcggtgcag actatgattc
    20521 tggcaacctg caaggcagga gataagatta ttctgccacg aaatgtccat aaatctgcta
    20581 tcaatgcgtt ggttctatgt ggtgccattc ccatctatat cgagatgagt gtagatccta
    20641 agattggtat cgctttaggt cttgaaaatg accgagtagc acaggccata aaggaccatc
    20701 cagatgctaa ggctatccta atcaacaatc ctacttacta cggcatctgt tcagacctaa
    20761 aggggttgac agaaatggct catgaagctg gcatgatggt tttagtagat gaagcccatg
    20821 gagcgcattt gcatttcact ggtaaacttc caatttctgc tatggatgca ggagctgata
    20881 tggcagcagt ttccatgcat aagtctggtg ggagtttgac acaaagttcg cttcttttaa
    20941 tcggggagca gatgaatccc gagtacgttc gtcagattat aaacctgacc cagtctacat
    21001 ctgcctctta cttattgatg gctagtttgg atatttcacg tcgcaacttg gcccttcgtg
    21061 gtaaagagtc gtttgaggaa gtcattgagc tatccgagta cgctcgtcat gaaatcaatg
    21121 ccattggcgg ttactatgct tactcaaaag agttaataga tggtgtgtcg gtctgtgatt
    21181 ttgatgtaac caagttgtca gtttacactc agggaatagg cttaacaggt atcgaggttt
    21241 atgacctctt gcgagacgaa tacgacattc agattgagtt tggtgatatc ggcaatatct
    21301 tggcctatat ttccatcggc gaccgcatcc aagacatcga gcgcttggtt ggtgctctgg
    21361 ctgatattaa gagactctat tcacgagatg ggaaggactt aatagctgga gaatatattc
    21421 agcccgagtt ggtgctgtct ccacaagaag ccttctattc agagagaaaa agtttgacct
    21481 tagatgagtc tgttggacag gtctgtggag aatttgtcat gtgctatcct ccagggattc
    21541 ctatcttggc tcctggtgaa cgtattacac gagaaattgt agactatatc caattcgcca
    21601 aggaacgtgg ttgctccctc caagggacgg aagatccaga ggtcaatcat atcaacgtta
    21661 ttaagagaaa gacaaactat aagaaaagtc aatagtttta tctaaactat ttcttatttc
    21721 aatttgatga tttggcgatg attttagagc acggcaaaaa gcccttgaat tagaagcggt
    21781 caatcgctta atttctatca gcttatcaaa tcctgcctca atccttttct gaggattagg
    21841 gtagcgtgtc aagagttggt aggtatattc tgaatgcttt ccaacgattt tatccaactc
    21901 aggaaagatg atatcaagac aacgagtgta ttgtactttc caatcagact gttttttctt
    21961 gagacgatga atatgtctag tcagtatttt tagttctact tgccgattat cgtgttgaaa
    22021 ttgttcacga ttggggtcag aaagaagttt aagagcgatg ccatgagcgt ctttcttatt
    22081 cgttttagtc ttgcgaagtg ataatgattt ggcaaattcc ttgatgagca aaggattgca
    22141 ggtgtaaact ttatatcctt gttcatgcag gaagttcagt agattaaagg cataatgtcc
    22201 ggtattttca agagcgatga gacagtcttg gttgagttgt cgaagagaca gatctaagag
    22261 ttcaaaacca gctttattat ttgaaaaagt gagtggttta agaacagttt ttcctggaac
    22321 attcaaggct gtaacatcgt gtttattttt agcgacatca atgcccacat aaagcatggg
    22381 agtacctcca gatgtagtat ttcaagtcta cttggttatc cacgaatttt ttgccttgtt
    22441 accttagacg agataaaacg tctatgcgtt atcaaactca ttaccaattg aaacaaaaaa
    22501 ctgtggttag agcctttcgg aaatcgtcaa gcgattggag ggaataaact aatccacagt
    22561 ggcttattcc aagtatacca cttgggcttt ggcagtagct aactgcgcta aatataatat
    22621 aaggagaaat agatggattt atggttttct gaagttcata ctccagatgt caaattgtct
    22681 ctgagaacag ccaagcaact ttacgctgga aaaagtgaat ggcaggatat cgaagtcttg
    22741 gatacgccag cttttgggaa aatactgatt ttaaatggcc atgtcttgtt ctcagatgcg
    22801 gatgatttcg tctacaatga aatgaccgtt cacgttccca tggctgtcca cccaaatcca
    22861 aagaaagtat tggttattgg gggtggtgac ggcggtgttg cccaagtatt aacactctat
    22921 cctgaactgg aacaaatcga tatcgtggaa ccggatgaga tgttggtcga ggtctgtcgt
    22981 gagtatttcc cagactttgc tgcagggcta gatgatcctc gtgttaccat ttactaccaa
    23041 aatgggctac gctttttgcg aaactgcgaa gatgattacg atattatcat caacgatgcg
    23101 acagatccat ttggccatac ggaaggactc tttaccaagg aattctacgg caatagttat
    23161 cgagctctga aggaagacgg catcatgatt taccagcatg ggagtccctt ctttgacgag
    23221 gatgagtcgg cctgccgaag catgcaccgc aaggtcaatc aatcctttcc aatcagtcgg
    23281 gtttatcagg cccatattcc aactagccca gctggctatt ggttgtttgg atttgcatcg
    23341 aaaaaatacc accctgtcaa agattttgac aaggaaggtt ggaaaaaacg ccagcttttc
    23401 acagaatact acactgcaaa cttacacgtg ggatccttta tgttgcccaa gtatgttgag
    23461 gacattttag aagaagagga aggaaaaaaa tgagtcgttt actagttatt ggttgtgggg
    23521 gcgttgccca agttgctatt tcaaagattt gtcaagatag cgaaacattt acagagatta
    23581 tgattgctag ccgtaccaag tcaaaatgcg atgacttgaa agcgaagcta gaaggcaaaa
    23641 caagtactaa aattgaaact gcagcacttg atgctgacaa ggttgaagaa gtgattgccc
    23701 tgattgaaag ctacaaacca gaagctgttt tgaatgtagc tctgccttat caagatttaa
    23761 ccattatgga tgcttgtttg gcaacaggtg ttcactatat cgatacagcc aactacgaag
    23821 cagaagacac agaagaccct gagtggcgtg ctatctacga aaaacgttgt aaggaacttg
    23881 gttttacagc ctactttgac tactcatggc agtgggctta tcaagagaaa ttcaaagaag
    23941 caggcttgac tgctcttctt ggttctggtt ttgacccagg tgtaactagt gtcttttcag
    24001 cttatgccct caaacactat tttgatgaaa tccattatat cgacatttta gactgtaatg
    24061 gcggtgacca cggttatcca tttgcaacca actttaatcc agaaattaat ctccgtgagg
    24121 tttctgctcc aggttcttac tgggaagatg ggaaatgggt cgaagtcgaa gctatgtcta
    24181 tcaagcgtga gtatgatttc cctcaagttg gacaaaaaga tatgtatctc cttcaccatg
    24241 aagaaatcga atcattggcc aagaacattc caggtgtcaa acgcattcgt ttctttatga
    24301 cttttggtca atcttacttg acgcacatga aatgtcttga aaatgttgga ctccttcgta
    24361 cggataccat taactttaac ggccaagaaa ttgttccaat tcaatttttg aaagccttgc
    24421 ttccagatcc tgccagtctt gggccacgta cagtcggaaa aaccaatatt ggatgtatct
    24481 ttacaggtgt caaagatggc gttgaaaaga ctatctacat ctacaatgtt tgcgaccatc
    24541 aagagtgcta tgcagaagtc gggtcacaag caatttcata cacgacaggc gttccagcca
    24601 tgattgggac aaaattagtc atgaacggaa cttggaaaca agctggagtg tataaccttg
    24661 aggagttaga tccagatcca ttcatggaag ctttgaatga gtatggtttg ccatgggttg
    24721 tggttgaaaa tccacaaatg gtggactaat gaagttagaa caagtaccaa caccagccta
    24781 tgttattgac ttggccaagt tagaagctaa ttgccgcatt ctacaatatg tacaagaaga
    24841 ggccggttgc aaggtcttgc ttgcccagaa ggcatattcc ctctacaaaa cttatccctt
    24901 gattagccag tatctatcag gtacgacagc tagtggactc tatgaggcca aattggcaag
    24961 ggaagaattt cctggtgaag tccatgtttt tgcaccagct ttcaaggatg aggacatgga
    25021 ggaattgctt gggataacag accatatcgt ctttaactca gagagacagt tgcgtaagca
    25081 tggggctcgt tgtcgagagg ctggtgtcag tgttggtttg cgcctcaacc ctcagtgttc
    25141 aactcaagga gatcacgcgc tctatgaccc ttgtgcacct ggttctcgct ttggagttac
    25201 tatagacaag attccgagtg atttgctaga tttggttgac ggacttcatt ttcataccct
    25261 ttgcgagcag ggagcagatg atttacaaac aactttgaaa gcagtagaag aacagtttgg
    25321 tccctactta catgaggtaa aatggctcaa tatgggtggt ggtcatcata ttacaagaga
    25381 aggttacgat gtggatttgc tgatttcaga aatcacgcgt atccgaaaaa cttacaatct
    25441 tgaaatctat atcgagcctg gtgaagccat tgcgcttaat gcgggttatt tagcaactga
    25501 ggtattagat attgtagaaa acggtatgga aatcttggtt ttagacgcct ctgcgacctg
    25561 ccatatgcct gatgtacttg agatgcccta tcgtccacct ttgagaaatg gctttgaggc
    25621 acaggaaaaa gcccatacct acagactttc ttctaatacc tgtctgacgg gcgatgtgat
    25681 tggtgattat agttttgaaa atccagtcca aatcggagac agactttatt ttcaagacat
    25741 ggccatttat tcttttgtca aaaataatac ctttaatggt attggattgc caagtctcta
    25801 tctcatggac gaacagggag actgtagctt actcaaagct tttggctatc aagactttaa
    25861 agggagatta tcatgatgga cagtccaaaa aaattaggct atcacatgcc agcagagtac
    25921 gaaccccatc atggtaccct catgatatgg ccgactcgac caggatcatg gccttttcaa
    25981 ggaaaggctg ctaaaagagc atttactcag atcatcgaga ccatagcaga aggggaaaga
    26041 gtctatcttt tggtggagca ggcctatcta tctgaagccc aatcctatct tggagacaag
    26101 gttgtttatt tagacattcc caccaatgat gcctgggcgc gtgatactgg cccaaccatt
    26161 ctcgtcaatg ataaaggtaa gaaattagcc gtggattggg ccttcaatgc ttggggaggc
    26221 acctatgatg gtctttatca agattatgaa gaggatgacc aagtagccag tcgttttgct
    26281 gaggtcttgg aaaagcctgt ctatgatgct aaaccttttg tactggaagg aggcgcaatc
    26341 catagcgatg gtcaaggaac tattctcgta actgaaagtt gcttgcttag tcctggtcgc
    26401 aatcctaacc tgactaaaga ggagattgaa aacacattat tagaaagtct tggtgctgaa
    26461 aaagttattt ggcttcctta tggtatttat caggatgaaa ccaatgaaca cgtcgataat
    26521 gttgctgcct ttgttggttc tgctgagctt gttttggctt ggacagatga cgaaaatgat
    26581 ccccagtatg ccatgtcaaa agcagatctc gaactcttag aacaggaaac agatgcaaaa
    26641 ggttgtcact tcaccattca taaattgcct atccctgcag ttcgacaagt tgtgacagaa
    26701 gaagatttgc caggctacat ctatgaagaa ggagaagaag agcgatacgc aggtgaacga
    26761 ctagcagctt cctacgtaaa cttttatatc gccaacaagg ctgtcttggt tccacagttt
    26821 gaggatgtaa acgaccaagt ggccatagat atcctcagca agtgtttccc agaccgtaaa
    26881 gttgtcggaa taccagccag agatattctc ttaggtggtg gtaatatcca ctgtatcacc
    26941 caacaaattc cagaatagga gaaaaagatg agaaatgtaa gagttgcaac cattcagatg
    27001 caatgcgcta aggatgtggc aacaaatatc caaaccgcag agcgtttagt acgtcaggct
    27061 gctgagcaag gagcccaaat tattctcttg cccgagttgt ttgaacatcc ttatttctgt
    27121 caggaacgtc agtatgacta ctaccagtat gcccaatctg tagcggaaaa tactgccatt
    27181 cagcatttta aggtgattgc taaggaacta caagttgttt taccaatcag tttctatgaa
    27241 aaagatggta atgtcttgta taactctatt gccgtcattg atgcagatgg ggaagtgctg
    27301 ggcgtttatc gaaagaccca cataccagat gaccattatt atcaagaaaa attctatttc
    27361 acgcctggta acactggttt caaggtctgg aatactcgct atgctaagat tggtatcggt
    27421 atctgttggg atcaatggtt ccctgaaaca gcgcgctgtc ttgcattgaa tggtgctgaa
    27481 ttgctctttt atcctacagc tatcggttca gagccaattt tggatacaga tagttgtggt
    27541 cactggcaac gtactatgca agggcacgca gcagcgaata ttgttccagt catcgcagcc
    27601 aatcgttatg gtttagagga ggttactcct agtgaggaaa atggcggaca gagttccagt
    27661 cttgacttct acggttcctc ctttatgacg gatgaaacag gagctattct agaacgagct
    27721 gaaagacaag aagaagctgt tctgttagct acttatgacc tagacaaggg agcaagtgaa
    27781 cgtctaaact ggggcttgtt tcgagataga agaccagaaa tgtatagaca aattacagat
    27841 tagtgtggga gaaatgagag attcattctg ctagactaac ttcttattag caactataag
    27901 atactatggc atctagtaaa tcgattttta tgattcgcta ttcttgtcta ttgattagtc
    27961 cgtattttaa aatattagca aaaaagcaaa tagcagtaac ttctgtctat ttgcttttct
    28021 tttttataga atatatttct caatagcacg cgcaacgccg tcttcttcgt tgcttgaggt
    28081 aacggcatcc gcaagagatt tgacataatt gctggcattt cccattgcaa tcccaagccc
    28141 tgcaaactgg agcatttcga tatcgttatt agcatcgccc atggccataa tctctgagga
    28201 atcaatcttc aaaatctcag ctagtcgtga aagagcagta gcctttgtcg ttccaagcgg
    28261 cattgcttca taaatgacag gctgcgaacg aactccactg aatcgttggc aaagctcttc
    28321 agcaaaacgc tgctcaaaat cgtctgtttg ttcttttgtt cctaaaaaca taccttggaa
    28381 catccggaac tttccactag tcgcttcttc aagagaaatt tcagtcaggt ctgaaaatac
    28441 tagtttagca tcattttcaa taacttgatt gggcttgcca ccgagaacaa aataatgtga
    28501 ctcgtcaaaa agtgtcaact gaacatcact cttttcagca aggtcataga ggtattcgat
    28561 gtcagctgga ctcagttctt gccagtcaac caagccccag tcgctagtct ggtgagttga
    28621 acaaccgttg ttaacaatca catactcatt ctggaggtcg agtcccagtt ttttgtagta
    28681 ggggaggaca ccgaaaaggg ggcgacccgt acagaggacc agtttgacac ctttttcaat
    28741 ggcttggtga atagcagcga tgtgagcctg tgggatttcc ttggcttcat tgaggagggt
    28801 tccatccata tccaaggcta gtagtttaat cataggtctt ccttctttat cttttggtat
    28861 tattatagca tattttggag aagaaattga tagaaagctt gagactaatt gattttatag
    28921 tttaagatgt tttgatgaca attcatgatt tgaagaggat atttggcaaa gatatgctat
    28981 actatgtttg tcaatgttgc aactagacaa attaaaaaac caacttaata taatagtttt
    29041 tttgt
//
LOCUS       DAVGJJ010000015        28871 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000015 DAVGJJ010000000
VERSION     DAVGJJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.015"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..182
                     /locus_tag="VVE35_000619"
     CDS             <1..182
                     /locus_tag="VVE35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU3060859.1"
                     /translation="LELIQGGADPYGKNPNGRYDWEIEPVLTLPVHGFCPRGTYDLGY
                     IGGGNHLCKGSAARF"
     gene            616..921
                     /locus_tag="VVE35_000620"
     CDS             616..921
                     /locus_tag="VVE35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3060860.1"
                     /translation="MLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSAIIFGYI
                     HINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLLLFSGS"
     gene            complement(973..1170)
                     /locus_tag="VVE35_000621"
     CDS             complement(973..1170)
                     /locus_tag="VVE35_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3060861.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1163..2071
                     /locus_tag="VVE35_000622"
     CDS             1163..2071
                     /locus_tag="VVE35_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU3060862.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2068..2529
                     /locus_tag="VVE35_000623"
     CDS             2068..2529
                     /locus_tag="VVE35_000623"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU3060863.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMVDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2519..3406
                     /locus_tag="VVE35_000624"
     CDS             2519..3406
                     /locus_tag="VVE35_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3060864.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKYRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3409..5292
                     /gene="cbpE"
                     /locus_tag="VVE35_000625"
                     /gene_synonym="pce"
     CDS             3409..5292
                     /gene="cbpE"
                     /locus_tag="VVE35_000625"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU3060865.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNIAQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKDQWYYLDVYGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5372..6502
                     /locus_tag="VVE35_000626"
     CDS             5372..6502
                     /locus_tag="VVE35_000626"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU3060866.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDCVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6512..7774
                     /locus_tag="VVE35_000627"
     CDS             6512..7774
                     /locus_tag="VVE35_000627"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU3060867.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7778..8575
                     /gene="proC"
                     /locus_tag="VVE35_000628"
     CDS             7778..8575
                     /gene="proC"
                     /locus_tag="VVE35_000628"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU3060868.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8795..9433
                     /locus_tag="VVE35_000629"
     CDS             8795..9433
                     /locus_tag="VVE35_000629"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU3060869.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9430..10320
                     /locus_tag="VVE35_000630"
     CDS             9430..10320
                     /locus_tag="VVE35_000630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU3060870.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10360..10677
                     /gene="yabA"
                     /locus_tag="VVE35_000631"
     CDS             10360..10677
                     /gene="yabA"
                     /locus_tag="VVE35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU3060871.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10680..11558
                     /gene="rsmI"
                     /locus_tag="VVE35_000632"
     CDS             10680..11558
                     /gene="rsmI"
                     /locus_tag="VVE35_000632"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU3060872.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASKGVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEENQEND"
     gene            11693..13027
                     /gene="trmFO"
                     /locus_tag="VVE35_000633"
     CDS             11693..13027
                     /gene="trmFO"
                     /locus_tag="VVE35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU3060873.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            13111..13848
                     /locus_tag="VVE35_000634"
     CDS             13111..13848
                     /locus_tag="VVE35_000634"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU3060874.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRSAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            13857..14414
                     /gene="frr"
                     /locus_tag="VVE35_000635"
     CDS             13857..14414
                     /gene="frr"
                     /locus_tag="VVE35_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU3060875.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            14474..15328
                     /gene="cvfB"
                     /locus_tag="VVE35_000636"
     CDS             14474..15328
                     /gene="cvfB"
                     /locus_tag="VVE35_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU3060876.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGIPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDLTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            15337..15552
                     /locus_tag="VVE35_000637"
     CDS             15337..15552
                     /locus_tag="VVE35_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU3060877.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            15638..16606
                     /locus_tag="VVE35_000638"
     CDS             15638..16606
                     /locus_tag="VVE35_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU3060878.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEETACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(16584..16763)
                     /locus_tag="VVE35_000639"
     CDS             complement(16584..16763)
                     /locus_tag="VVE35_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060879.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            16799..17299
                     /locus_tag="VVE35_000640"
     CDS             16799..17299
                     /locus_tag="VVE35_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060880.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            17302..17562
                     /locus_tag="VVE35_000641"
                     /pseudo
     CDS             17302..17562
                     /locus_tag="VVE35_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(17929..18255)
                     /locus_tag="VVE35_000642"
     CDS             complement(17929..18255)
                     /locus_tag="VVE35_000642"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060881.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(18201..18614)
                     /locus_tag="VVE35_000643"
     CDS             complement(18201..18614)
                     /locus_tag="VVE35_000643"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU3060882.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(18615..19040)
                     /locus_tag="VVE35_000644"
     CDS             complement(18615..19040)
                     /locus_tag="VVE35_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU3060883.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            19217..19786
                     /locus_tag="VVE35_000645"
     CDS             19217..19786
                     /locus_tag="VVE35_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060884.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLVNPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     SNKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            19854..20504
                     /locus_tag="VVE35_000646"
     CDS             19854..20504
                     /locus_tag="VVE35_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU3060885.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     VEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            20488..22728
                     /locus_tag="VVE35_000647"
     CDS             20488..22728
                     /locus_tag="VVE35_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU3060886.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            22907..23095
                     /locus_tag="VVE35_000648"
     CDS             22907..23095
                     /locus_tag="VVE35_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060887.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            23127..23714
                     /locus_tag="VVE35_000649"
     CDS             23127..23714
                     /locus_tag="VVE35_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060888.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            23718..24899
                     /locus_tag="VVE35_000650"
     CDS             23718..24899
                     /locus_tag="VVE35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060889.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    25059..25190
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            25206..25736
                     /gene="infC"
                     /locus_tag="VVE35_000651"
     CDS             25206..25736
                     /gene="infC"
                     /locus_tag="VVE35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU3060890.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            25769..25969
                     /gene="rpmI"
                     /locus_tag="VVE35_000652"
     CDS             25769..25969
                     /gene="rpmI"
                     /locus_tag="VVE35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU3060891.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            26021..26380
                     /gene="rplT"
                     /locus_tag="VVE35_000653"
     CDS             26021..26380
                     /gene="rplT"
                     /locus_tag="VVE35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU3060892.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            26438..26818
                     /locus_tag="VVE35_000654"
     CDS             26438..26818
                     /locus_tag="VVE35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3060893.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            27068..27868
                     /locus_tag="VVE35_000655"
     CDS             27068..27868
                     /locus_tag="VVE35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU3060894.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            27879..28817
                     /locus_tag="VVE35_000656"
     CDS             27879..28817
                     /locus_tag="VVE35_000656"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU3060895.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACLDIIENLPKVMDKYGISSLEELRQEVKESLR"
BASE COUNT         8560 a         5256 c         6709 g         8346 t
ORIGIN      
        1 aattagagct gatacaaggt ggagcagatc catatggtaa aaatcctaat ggtaggtacg
       61 attgggaaat agaaccagta ttaactctgc cggttcatgg attttgtccc agaggcacct
      121 atgatttagg atatattgga ggaggcaatc atctttgcaa aggaagtgct gcgagatttt
      181 aagtaaaatt tattagtaat atgaagaaac aaggggagaa agcagagaat ttaatatgaa
      241 aaaacgggct attcaaattt tactagcatt gtccttaatt ttttacaaat caacttggtt
      301 ttggagagtt ttcaatcacc tcgcaaagcc ctatctacca gcaagtcgtg aattttttca
      361 gattctgctt ttgctggaaa gtggtgttct tttgtcagca gtcatctatc tactagtttt
      421 tgcaggaaag aagatttttc atttcaagtg gcagttgagg tactttatct accttttact
      481 gggctacatc atttcgtata tgtctgactt tctcttttcg tatttcatat ccccgccttc
      541 aaatccccgc cttcaaatca aatttctttg aatgaaacga tagaaatgat gggacgacag
      601 gagttccctt atttcttgct catcgtttgc ttcatcgccc ctattgctga ggaattgatt
      661 tatcgcggtg tgcttatgac aacctttttc aaaaactcgc cttggtacgg agatgttttg
      721 ctttctgcta ttattttcgg ttatattcat atcaattttg ctttaactcc tcttgctttt
      781 ttcatttatg ctagtggagg tcttatttta gctctattgt atcgcatgac taaaaatctc
      841 tactatccaa tactagttca tattctcatc aatatcactg ccttctggga tgtttggtta
      901 ctcctatttt caggaagtta gcttactaaa aaaatgtcgg aattttccgg cattttcttt
      961 tttcacaaat agtcaacgtt tttctttccg atactgaagt ggtgtgtagc cacttatttt
     1021 tttgaattga ttttgaaaat aagattggcg tgagaaaggc agatagtgaa gatagttaag
     1081 aagaatagga tgttcttttt tcctttttgg aaaacttcta aaatatggta taatgaaaag
     1141 ataaagaagt tgggggtaga agatgaacat tcaacaatta cgctatgttg tggctattgc
     1201 caatagtggt acttttcgtg aagctgctga aaagatgtat gttagtcagc cgagtctgtc
     1261 tatttctgtt cgtgatttgg aaaaagagtt gggctttaag attttccgtc ggaccagctc
     1321 agggactttc ttgacccgtc gtgggatgga attttatgaa aaagcgcaag aattggttaa
     1381 aggatttgat atttttcaaa atcagtatgc caatcctgaa gaagaaaaag atgaattttc
     1441 cgttgctagc cagcactatg acttcttacc accaactatt acggcctttt cagagcgcta
     1501 tcctgactat aagaacttcc gtatttttga atcaactact gttcaaatat tagatgaagt
     1561 ggcgcaaggg catagtgaga ttgggattat ctacctcaac aatcaaaata aaaaggggat
     1621 tatgcaacgg gttgaaaagt taggtctgga ggtcatcgaa ttgattcctt tccataccca
     1681 tatttatctc tgtgagggtc atcctttagc ccagaaagag gaattagtca tggaggattt
     1741 agcggattta ccaacggttc gtttcactca agagaaagac gagtaccttt attattcaga
     1801 gaactttgtt gataccagcg ctagctcaca gatgtttaat gtgacagacc gtgccacctt
     1861 gaatggtatt ttggagcgga cggacgccta tgcgacaggt tctggatttt tagatagtga
     1921 cagtgttaat ggcattacag ttattcgtct caaggataac ctagataacc gcatggtcta
     1981 tgttaaacgt gaagaagtgg agcttagtca agctgggact ctcttcgtag aagtcatgca
     2041 agaatatttt gatcaaaaga ggaaatcatg aaaaaaagag caatagtggc agtcattgta
     2101 ctgcttttga ttgggctgga tcagttggtc aaatcctata tcgtccagca gattccactg
     2161 ggtgaagtgc gctcctggat ccccaatttc gttagcttga cctacctgca aaatcgaggt
     2221 gcagcctttt ctatcttaca agatcagcag ttgttattcg ctgtcattac tctggttgtc
     2281 gtgataggtg ccatttggta tttacataaa cacatggtgg actcattctg gatggtcttg
     2341 ggtttgactc taataatcgc gggtggtctt ggaaacttta ttgacagggt cagtcagggc
     2401 tttgttgtgg atatgttcca ccttgacttt atcaactttg caattttcaa tgtggcagat
     2461 agctatctga cggttggagt gattatttta ttgattgcaa tgctaaaaga ggaaataaat
     2521 ggaaattaaa attgaaactg gtggtctgcg tttggataag gctttgtcag atttgtcaga
     2581 attatcacgt agtctcgcga atgaacaaat taaatcaggc caggtcttgg tcaatggtca
     2641 agtcaagaaa gctaaataca cagtccaaga gggtgatgtc gtcacttacc atgtgccaga
     2701 accagaggta ttagagtatg tggctgagga tcttccgcta gaaatagtct accaagatga
     2761 ggatgtggct gtcgttaaca aacctcaggg aatggttgtg cacccgagtg ctggtcatac
     2821 cagtggaacc ctagtaaatg ccctcatgta tcatattaag gacttgtcgg gtatcaatgg
     2881 ggttctgcgt ccagggattg ttcaccgtat tgataaggat acgtcaggtc ttctcatgat
     2941 tgctaaaaac gatgatgcgc atctagcact tgcccaagaa ctcaaggata aaaagtctct
     3001 ccgcaaatat tgggcgattg ttcatggaaa tctgcctaat gatcgtggtg taattgaagc
     3061 gccgattggc cggagtgaaa aataccgtaa gaaacaggct gtaactgcta aagggaagcc
     3121 tgcagtgacg cgttttcacg tcttggaacg ctttggcgat tatagcttag tagagttgca
     3181 actggagaca gggcgcactc atcaaatccg tgtccacatg gcttatatcg gccatccagt
     3241 cgctggtgat gaggtctatg gtcctcgcaa gactttgaaa ggacatggac aatttcttca
     3301 tgccaagact ttaggtttta ctcatccgag aacaggtaag accttggaat ttaaagcaga
     3361 tatcccagag atttttaagg aaaccttgga gagattgaga aagtaagaat gaaaaagaaa
     3421 ttaactagtt tagcacttgt aggcgctttt ttaggtttgt catggtatgg gaatgttcag
     3481 gctcaagaaa gttcaggaaa taaaatccac tttattaatg ttcaagaagg tggcagtgat
     3541 gcgattattc ttgaaagcaa tggacatttt gccatggtgg atacaggaga agattatgat
     3601 ttcccagatg gaagtgattc tcgctatcca tggagagaag gaattgaaac gtcttataag
     3661 catgttctaa cagaccgtgt ctttcgtcgt ttgaaggaat tgggtgtcca aaaacttgat
     3721 tttattttgg tgacccatac ccacagtgat catattggaa atgttgatga attactgtct
     3781 acctatccag ttgaccgagt ctatcttaag aaatatagtg atagtcgtat tactaattct
     3841 gaacgtctat gggataatct gtatggctat gataaggttt tacagactgc tgcagaaaaa
     3901 ggtgtttcag ttattcaaaa tatcgcacaa ggggatgctc attttcagtt tggggacatg
     3961 gatattcagc tctataatta tgaaaatgaa actgattcat cgggtgaatt aaagaaaatt
     4021 tgggatgaca attccaattc cttgattagc gtggtgaaag tcaatggcaa gaaaatttac
     4081 cttgggggcg atttagataa tgttcatgga gcagaagaca agtatggtcc tctcattgga
     4141 aaagttgatt tgatgaagtt taatcatcac catgatacca acaaatcaaa taccaaggat
     4201 ttcattaaaa atttgagtcc gagtttgatt gttcaaactt cggatagtct accttggaaa
     4261 aatggtgttg atagtgagta tgttaattgg ctcaaagaac gaggaattga gagaatcaac
     4321 gcagccagca aagactatga tgcaacagtt tttgatattc gacaagacgg tttagtaaac
     4381 atttcaactt cctacaagcc gattccaagt tttcaagctg gttggcataa gagttcatat
     4441 ggaaactggt ggtatcaagc gcctgattct acaggagaat atgctgtcgg ttggaatgaa
     4501 attaagggtg aatggtatta ctttaaccaa acgggtatct tattacagaa tcaatggaaa
     4561 aaatggaaca atcgttggtt ctatttgaca gactctggtg ttgctgctaa aaattggaag
     4621 aaaatcgctg gaatctggta ttattttaac aaagaaaacc agatggaaat tggttggatt
     4681 caagataaag atcagtggta ttatttggat gtttatggct ctatgaagac aggttggctt
     4741 caatataagg ggcaatggta ttactttgct ccatcagggg aaatgaaaac gggctgggta
     4801 aaagataaag aagcctggta ctatatggat tctactggta tcatgaagac aggtgagata
     4861 gaagttgctg gacatcatta ctatctagaa gaatcaggag ctatgaagca aggctggctt
     4921 aaaaaggcaa atgattggta tttctacaag acagatggtt cacgagctat aggttggatt
     4981 aaagacaagg ataaatggta cttcttgaaa gaaaatggtc aattacttgt gaatggtaag
     5041 acaccagaag gctatactgt ggattcaagt ggtgcctggt tagtggatgt tccgatcgag
     5101 aaatctgcta caattaaaac tacaagtcat tcagaaataa aagaatccaa agaagtagtg
     5161 aaaaaggatc ttgaaaataa agaaacgagt caacatgaaa gtgttacaaa ttcttcaact
     5221 agtcaagatt tgacatcctc aacttcacaa agctctgaaa cgagtgtaaa caaatcggaa
     5281 tcagaacagt agtagaaaag aaggttttag ggccttcttt ttcctatcaa ctcttttcta
     5341 tttcctgtta ttcatgttat aatggataaa tatgaataat cggagtgaga ctatgaaata
     5401 caaacggatt gtctttaagg tgggtacttc ttctctgaca aatgaggatg gaagtttatc
     5461 acgtagtaag gtaaaggata ttacccagca gttggctatg ctgcacgagg ctggtcatga
     5521 gttgattttg gtgtcttcag gtgccattgc ggctggtttt ggagccttag gatttaaaaa
     5581 gcgtccgact aagattgctg ataaacaggc ttcagcagcg gtagggcagg gacttttgtt
     5641 ggaagaatat acgaccaatc ttctcttgcg tcaaatcgtt tctgcacaaa tcttgctgac
     5701 ccaagatgac tttgtggata agcgtcgtta taaaaatgcc catcaggctt tgtcggtttt
     5761 actcaaccgt ggggcaattc ctatcatcaa tgagaatgat tgtgtcgtta ttgatgaggt
     5821 caaggttggg gacaatgaca ctctaagtgc ccaagtagcg gcgatggtcc aagcagacct
     5881 tttggttctc ttgacagatg tggacggtct ctatactgga aatcctaatt cagatccaag
     5941 agccaaacgc ttggagagaa tcgagaccat caatcgtgag attattgata tggctggtgg
     6001 agctggttcg tcaaacggaa ctgggggtat gttaaccaaa atcaaggctg caactatcgc
     6061 gacggaatca ggagttcctg tttatatctg ctcatccttg aaatcagatt ccatgattga
     6121 ggcggcagag gagaccgagg atggctctta ctttgttgct caagagaagg ggcttcgtac
     6181 ccagaaacaa tggcttgcct tctatgctca gagtcaaggt tctatttggg ttgataaagg
     6241 ggctgcggaa gctctctctc aacatggaaa gagtctcctc ttatctggta tcgttgaagc
     6301 agaaggagtc ttttcttacg gtgatatcgt gacagtattt gataaggaaa gtggaaaatc
     6361 acttggaaaa ggacgcgtgc aatttggagc atctgctttg gaggatatgt tgcgttctca
     6421 aaaagccaag ggtgtcttga tttaccgtga cgactggatt tccattactc ctgaaatcca
     6481 actacttttt acagaatttt agaggtaaac tatggtgagt agacaagaac aatttgaaca
     6541 ggtacaggct gttaaaaaat cgattaacac agctagtgaa gaagtgaaaa accaagcctt
     6601 gctagccatg gctgatcact tagtggctgc tactgaggaa attttagcgg ctaatgccct
     6661 cgatatggca gcggctaagg ggaaaatctc agatgtgatg ttggatcgtc tttatttgga
     6721 tgcagatcgt atagaagcga tggcaagagg aattcgtgaa gtggttgcct taccagatcc
     6781 aatcggtgaa gttttagaaa caagtcagct tgaaaatggt ttggttatca caaaaaaacg
     6841 tgtagctatg ggtgtcatcg gtattatcta tgaaagccgt ccaaatgtga cgtctgatgc
     6901 ggctgctttg actcttaaga gtggaaatgc ggttgttctt cgtagtggta aggatgccta
     6961 tcaaacaacc catgccattg tcacagcctt gaagaagggc ttggagacga ctactattca
     7021 tccaaatgtg attcaactgg tggaggatac tagccgtgaa agtagttatg ctatgatgaa
     7081 ggccaagggc tatctagacc ttctcattcc tcgtggagga gctggcttga tcaatgcagt
     7141 ggttgagaat gcgattgtac ctgttatcga gacagggact gggattgtcc atgtctatgt
     7201 ggataaggat gcagacgaag acaaggcgct gtctatcatc aacaatgcta aaaccagtcg
     7261 tccttctgtt tgtaatgcca tggaggttct gctggttcat gaaaacaaag cagtaagctt
     7321 ccttcctcgc ttggagcaag tgttggttgc agatcgaaaa gaagctgggt tggaaccaat
     7381 tcaattccgc ctagatagca aagcaagcca gtttgtttca ggtcaagctg ctcaagcaca
     7441 agactttgat accgagtttt tagactatat tctagctgtt aaggttgtga gcagtttaga
     7501 agaagcggtt gcgcatattg aatcccacag tacccatcat tcggatgcca ttgtgacgga
     7561 aaatgctgaa gctgcagcat actttacaga tcaagtggac tctgcagcgg tgtatgttaa
     7621 tgcctcaact cgtttcacag atggaggaca atttggtctg ggttgtgaaa tggggatttc
     7681 tactcagaaa ttgcatgcgc gtggtccaat gggcttgaaa gagttgacca gctacaagta
     7741 tgtggttgct ggtgatgggc agataaggga gtaagagatg aagattggat ttatcggttt
     7801 ggggaatatg ggtgctagct tggcaaaatc tgtcttgcag actaggacgt cagatgagat
     7861 tctccttgcc aatcgtagtc aagctaaggt agatgctttc attgcagact ttggtggtca
     7921 ggcttccagc aatgaagaaa tgtttgcaga agcagatgtg atttttctag gagttaagcc
     7981 tgctcagttt tctgaactgc tttctcaata ccagaccatc cttgaaaaaa gagaaagtct
     8041 tcttttgatt tcgatggcag ctggattgac cttagaaaaa ctagcaagtc ttatcccaag
     8101 ccaacaccga attattcgta tgatgcctaa tacccctgct tctatcgggc aaggagtgat
     8161 tagttatgcc ttgtctccta attgcagggc tgaggacagt gagatctttt gtcagctttt
     8221 agccaaggct ggtctcttgg ttgaattagg agaaagctta atcaatgcag cgacaggtct
     8281 tgcaggttgt ggaccagcct ttgtctatct ctttattgag gccttggcag atgcaggtgt
     8341 tcagacggga ttaccacgag aaacagcctt gaaaatggca gcccaaactg tggtaggagc
     8401 tgggcaattg gtcctagaaa gccagcaaca tcctggagtt ttgaaagatc aagtttgtag
     8461 tccaggcggt tcgactattg ctggcgtagc aagcctagaa gcgcatgctt ttcgaggcac
     8521 agtcatggag gcagttcatc aagcctataa acgaacacaa gaactaggta aataagaggt
     8581 agttttatct gcctctttta tggtggttga tactcttcga aaatcaaatt caaactgcgt
     8641 cagcttcacc ttgccgtact caagtacagc ctgcggctag cttcctagtt tgctctttga
     8701 ttttcattga gtatgaaatg agaagacaaa aagattgtca caaaccccta tttttttgat
     8761 agaatagaag tagtaaaaaa gaaatgagtt agacatgtca aaaggatttt tagtctctct
     8821 tgagggacca gagggagcag gtaagaccag tgttttagag gctctgctac caattttaga
     8881 ggaaaaagga gtagaggtgc tgacgacccg tgaacctggc ggagtcttga ttggggagaa
     8941 gattcgggaa gtgattttgg atccaagtca tactcagatg gatgctaaaa cagagctact
     9001 tctctatatt gccagtcgca gacagcattt ggtggaaaaa gttcttccag cccttgaagc
     9061 tggcaagttg gtcatcatgg atcgttttat cgatagttct gttgcctatc agggatttgg
     9121 tcgtggctta gatattgaag ccattgactg gctcaatcag tttgcgacag atggcctcaa
     9181 acccgatttg acactctatt ttgacatcga ggtggaagaa gggctggctc gtattgctgc
     9241 gaatagtgac cgcgaggtta atcgtttgga tttggaaggg ttggacttgc ataaaaaagt
     9301 tcgtcaaggc tacctttctc ttctggataa agagggaaat cgcattgtca agattgatgc
     9361 tagtctccct ttggagcaag ttgtggaaac taccaaggct gtcttgtttg acggaatggg
     9421 cttggccaaa tgaaacaaga tcaactaaag gcttggcaac cagctcagtt tgaccgtttt
     9481 gtccgtatct tagaacaaga ccagctcaat cacgcctatc tcttttcagg tttctttgga
     9541 agcttggaaa tagcgcaatt tttagctaag agcctctttt gtacggataa agttggcgtc
     9601 ttaccatgtg agaaatgccg aagttgcaag ctgattgaac aggaagagtt tccagatgtc
     9661 accttgatta agccagtcaa tcaggtcatc aagacagaac gtattaggga attggtggga
     9721 cagttttctc aagcagggat tgaaagccag caacaggtct ttattatcga gcaagcggat
     9781 aaaatgcatc ccaacgcagc caattctctg ctcaaggtca tcgaagaacc ccagagtgaa
     9841 gtttatattt tcttcttgac tagcgatgag gaaaagatgt taccgacaat ccgaagtcgg
     9901 actcagatct tccactttaa aaagcaagaa gaaaaactta tcttactctt agaacaaatg
     9961 ggacttgtta agaaaaaagc gactctttta gctaagttta gtcaatcgcg agctgaagca
    10021 gaaaagttgg ctaatcaggc aagtttttgg accttggtcg atgaaagtga acgcctgctg
    10081 acttggttag tagctaagaa aaaagaaagt tatctacagg ttgccaaatt agccaacttg
    10141 gcagatgata aggaaaaaca ggatcaggtt ttacggattc ttgaagttct ctgcgggcag
    10201 gacctcttgc aggtaagagt aagagtgatt ctacaagatt tactagaagc tagaaaaatg
    10261 tggcaagcta atgtcagctt tcaaaatgcc atggaatatc tggtcttgaa agaaatataa
    10321 actcaaaaat gaatgataaa gaaaggaaag ggctgtttta tggacaaaaa agaattattt
    10381 gacgcgctgg atgatttttc ccaacaatta ttggtaacct tagccgatgt ggaagccatc
    10441 aagaaaaatc tcaagagctt ggtagaagaa aatacagctc ttcgcttgga aaatagtaag
    10501 ttgcgagaac gcttgggtga ggtggaagca gacgctcccg tcaaggccaa gcatgttcgc
    10561 gaaagtgtcc gtcgtattta ccgtgatgga tttcacgtat gtaatgattt ttatggacaa
    10621 cgtcgagagc aggacgaaga atgtatgttc tgtgatgagt tgttatacag ggagtaggca
    10681 tgcaaattca aaaaagtttt aaggggcagt ctccctatgg caagctgtat ctagtggcaa
    10741 cgccgattgg caatctagat gatatgactt ttcgtgctat ccagaccttg aaagaagtgg
    10801 actggattgc tgctgaggat acgcgcaata caggtctttt gctcaagcat tttgacattt
    10861 ccaccaagca gatcagtttt catgagcaca atgccaagga aaaaattcct gatttgattg
    10921 gtttcttgaa agcagggcaa agtattgctc aggtctctga tgccggtttg cctagcattt
    10981 cagaccctgg tcatgattta gttaaggcag ctattgagga agaaattgca gttgtgactg
    11041 ttccaggtac ctctgcagga atttctgcct tgattgccag tggtttagcg ccacagccac
    11101 atatctttta cggtttttta ccgagaaaat caggtcaaca gaagcaattt tttggctcta
    11161 aaaaagatta tcctgaaaca cagatttttt atgaatcgcc ccatcgtgtg gcagatacat
    11221 tggaaaatat gctagaagtc tacggtgacc gctcggtagt cttggttaga gaattgacca
    11281 aaatctatga agaataccaa agaggtacaa tttctgaatt gctggaaagc atctctgaaa
    11341 cgtctctcaa gggtgaatgt cttctgattg ttgaaggtgc cagcaaaggt gtggaggaaa
    11401 aggatgagga agacttgttc ttagaaatcc aagcccgtat ccagcaaggc atgaagaaaa
    11461 atcaagctat taaggaagtc gctaagattt accagtggaa taaaagtcag ctctacgctg
    11521 cctaccacga ctgggaagaa aatcaagaaa atgactaaac agggaagttt gccttcttcc
    11581 ctttttttgt tatactagta gaagaaaaaa ttagaaagat ttgtgggtgt taaacaagcc
    11641 agtgccttgt tttgatatga acttaggttc cacccaaaga ggtattagtg tcgtgtctca
    11701 atcttatatc aatgttatcg gtgctggttt ggcaggttct gaagcagctt accaaatcgc
    11761 agagcgtggt attccagtta aactatatga aatgcgtggt gtcaagtcta caccccagca
    11821 taaaacagac aattttgctg agttggtttg ttccaattct ttgcgtgggg atgctttgac
    11881 aaatgcagtt ggtcttctca aggaagaaat gcgtcgcttg ggttctgtta tcttggaatc
    11941 tgctgaggct acacgtgttc ctgccggtgg agcccttgcg gtggatcgtg atggtttctc
    12001 tcaaatggtg accgaaaaag tggtcaacca ccccttgatt gaagtggtgc gtgatgaaat
    12061 tacagaatta ccaacagatg ttattacagt tgtggcgact ggtcccttga ctagcgatgc
    12121 cttggctgaa aagattcatg ctcttaatga tggcgatggc ttctatttct acgatgcagc
    12181 agcgcctatt atcgatgtca acactatcga tatgagcaag gtctatctca aatctcgtta
    12241 tgataaggga gaagcagcct atctcaatgc tccaatgacc aagcaagagt ttatggattt
    12301 ccatgaagcc ttggtcaatg cggaagaagc accgctcaat tcttttgaaa aagaaaagta
    12361 ctttgaagga tgtatgccaa tcgaagttat ggccaaacgt ggcattaaaa ctatgcttta
    12421 tggccctatg aagccagtcg gtcttgagta tccggacgac tacacaggac ctcgtgatgg
    12481 agaatttaaa acaccttatg cggttgtgca acttcgtcag gataatgcag ctggtagcct
    12541 atacaatatt gttggtttcc agacccacct caaatgggga gaacaaaagc gtgtcttcca
    12601 aatgattccg ggtcttgaaa atgcggagtt tgtccgttat ggtgtcatgc atcgcaattc
    12661 ttacatggat tcaccaaatc ttcttgagca gacttaccgt tccaagaaac aaccaaatct
    12721 cttctttgct ggtcagatga cgggggtgga aggctatgtt gagtcggcgg cttcgggctt
    12781 agttgcggga attaacgcag ctcgtctctt caaggaagaa agcgaggtta ttttccccga
    12841 gacgacagcg attggaagct tagctcatta cattacccat gccgacagca aacatttcca
    12901 accaatgaat gtcaattttg ggatcatcaa ggagttggaa ggcgagcgta tccgtgataa
    12961 gaaggctcgt tatgaaaaaa ttgcagagcg tgcccttgcc gacttagagg aatttttgac
    13021 tgtctaattt ttttgaaaga attgctcatt atactataaa aatcttagaa attgtgatag
    13081 aataggtagg atgaaagaag gagagtgaaa atggcgaatc ccaagtataa acgtatttta
    13141 atcaagttat caggtgaagc ccttgccggt gaacgtggcg tagggattga tatccaaaca
    13201 gttcaaacaa tcgcaaaaga gattcaagaa gttcatagct taggtatcga aattgccctt
    13261 gttatcggtg gaggaaatct ctggcgtgga gaacctgcag cagaagcagg tatggaccgt
    13321 gttcaggcag attacactgg aatgcttggg actgttatga atgctcttgt gatggcagat
    13381 tcattgcaac aagttggggt tgatacacgt gttcaaacag ctattgccat gcaacaagtg
    13441 gcagagcctt atgtacgtgg acgtgctctt cgccaccttg aaaaaggccg tatcgttatc
    13501 tttggtgctg gaattggttc accttacttc tcaaccgata caacagcggc tcttcgttca
    13561 gctgaaatcg aagcggatgc cattcttatg gctaaaaatg gtgtcgatgg tgtgtacaat
    13621 gccgatccta agaaggataa gacagctgtt aagtttgaag aattgaccca ccgtgacgtt
    13681 atcaataaag gtcttcgtat catggactca acagcttcaa ctctctcaat ggacaacgac
    13741 attgacttgg ttgtcttcaa catgaaccaa tcaggcaaca tcaaacgtgt cgtatttggt
    13801 gaaaatatcg gaacaacagt ttcaaataat atagaagaaa aggaataaga aagaatatgg
    13861 ctaacgcaat tattgaaaaa gctaaagaga gaatgaccca gtctcaccaa tcacttgctc
    13921 gtgaatttgg tggtatccgt gctggccgtg ccaatgcaag cttgctcgac cgtgtacatg
    13981 tagaatacta tggagtcgaa actcctctta atcaaatcgc ttcaattacg attccagaag
    14041 cgcgtgtttt gttggtaaca ccatttgaca agtcttcatt gaaggacatc gaacgtgctt
    14101 tgaacgcttc tgatcttggt atcacaccgg ctaatgacgg ttctgtgatt cgcttggtta
    14161 tcccagctct tacagaagaa actcgtcgtg accttgctaa agaagtgaag aaggtcggcg
    14221 aaaatgctaa agtggctgtc cgcaatatcc gtcgcgatgc tatggacgaa gctaagaaac
    14281 aagaaaaagc aaaagaaatc actgaagacg aattgaagac tcttgaaaaa gacattcaaa
    14341 aagtaacaga cgatgctgtt aaacacatcg acgatatgac tgccaacaaa gagaaagaac
    14401 ttttggaagt ctaaaaataa acagaaaaac tcagttggca ttgctggctg agttttattc
    14461 gaaagaagga aatatgaata caaatcttgc aagttttatc gttggactga tcatcgatga
    14521 aaatgaccgt ttttactttg tgcaaaagga tggtcaaact tatgctcttg ctaaggaaga
    14581 aggtcaacat acagtagggg atacggtcaa aggttttgca tacacggata tgaagcaaaa
    14641 actccgcctg acaaccttag aagtgactgc cactcaggac caatttggtt ggggacgtgt
    14701 cacagaggtt cgtaaggatt tgggtgtctt tgtggataca ggcattcctg acaaggaaat
    14761 cgttgtgtca ctcgatattc tccctgagct caaggaactc tggcctaaga agggcgacca
    14821 actctacatc cgtcttgaag tggataagaa agaccgtatc tggggcctct tggcttatca
    14881 agaagacttc caacgtcttg ctcgtcctgc ttacaacaac atgcagaacc aaaactggcc
    14941 agccattgtt taccgtctca agctgtcagg aacttttgtt tacctaccag aaaataatat
    15001 gcttggtttt attcatccta gcgagcgtta cgcagagcca cgtttggggc aagtattaga
    15061 tgcgcgcgtt attggtttcc gtgaagtgga cctcactctg aacctctccc tcaaaccacg
    15121 ctcctttgaa atgttggaaa acgatgctca gatgattttg acttatttgg aaagcaatgg
    15181 cggtttcatg accttaaatg ataagtcatc tccagacgac atcaaggcaa cctttggcat
    15241 ttctaaaggt cagttcaaga aagctttagg tggtcttatg aaggctggta aaatcaagca
    15301 agaccagttt gggacagagt tgatttaggg agatttatga gaaaatcatt ttacacttgg
    15361 ctcatgaccg agcgcaatcc taaaagtaac agtcccaaag ctatcttggc agacctcgct
    15421 tttgaagagg cagcttttcc aaaacacaca gatgattttg atgaggtcag tcgctttttg
    15481 gaggagcatg ccagtttctc ttttaaccta ggagactttg acgctatctg gcaagaatac
    15541 ttagaacact agcatttatt aactaggttt gggctagtaa tttctccatc cctctgctat
    15601 aataaaaaga aataaaagga ttagagaggt tctttatttg aaggaacatt caatagacat
    15661 tcaactgagt catccagatg acttgtttca tctttttggt tccaatgaac gccatcttcg
    15721 tttgatggaa gaagagctgg atgtggtgat tcatgctcgt acggagattg tccaggtttt
    15781 gggagaagag actgcctgtg aggaagcccg tcaggttatc caagctttga tggtcttggt
    15841 gaaccgtggg atgaccgttg gtacgccaga tgtggtgact gcaattagca tggtcaaaaa
    15901 cgatgaaatt gataagtttg tcgcccttta cgaagaagaa attatcaagg ataatactgg
    15961 gaaaccgatc cgcgttaaaa cgttaggtca aaaactttat gtggacagtg tcaaacagca
    16021 tgatgtgacc tttggaattg ggcctgcagg gacggggaag acctttcttg cagttacttt
    16081 ggcagtgact gcccttaaac gtgggcaagt caagcggatt atcttgacac gtccagcggt
    16141 ggaagcagga gagagtctag gttttcttcc gggtgatctt aaggagaagg tagatcctta
    16201 ccttagacct gtttatgatg ccttgtatca gattctgggg aaagaccaaa caactcgact
    16261 catggagcgt gaaattatcg agattgcgcc ccttgcctat atgcgtggac ggaccttgga
    16321 tgatgccttt gtcattctcg atgaggcgca aaatacgacc atcatgcaga tgaagatgtt
    16381 cttgactcgt ttaggttttc attctaagat gattgtcaat ggagatatta gtcagattga
    16441 cctgccacgt aatgtcaagt ccggtttgat tgatgctcaa gagaaactca agaacatcca
    16501 tcagattgac tttgttcatt tttcagccaa ggatgtggtt cgccatccag ttgtcgctca
    16561 gattatccga gcctatgaat attctactga agttgcacac gactgatttt gaggaagttc
    16621 gcctgcaaaa gaatatactt ttgagagagg aaaatccagt tgtataggct aaaggtttta
    16681 tccaaaggtt tgagacaacg attaggcacg atggaaagaa cttttatgtg gctgatgacg
    16741 atcagtgcat cttcctgtgt cataatcaca gggcacaaga aagtaggaat ttgaaaagat
    16801 gattgaccaa ctatctaagt attacagttg taggatacta actgaaaagg atattccaag
    16861 tattttatct ttatatgaaa gtaatcctct gtattttcag cattgtccac cagagccaaa
    16921 ttttgcaact gtaaaagagg acatgctttg tctacctgaa ggtaaagcta aggctgataa
    16981 gttttttgtt ggattttgga atggatctga ccttgtggct gttatggatt ttgtctatgc
    17041 atatcctgat gaggagactg tttttattgg tttgtttatg gttgatcaag cctatcagag
    17101 gaaagggatt ggtagtcata ttgtgacaga agcactagct tattttgcta agaactttcg
    17161 aaaggcacgt ttggcttatg ttaagggaaa tccgcaatct cagcattttt gggaaaagca
    17221 gggctttaaa tcaattggat gcgaggttaa gcaagaactc tatacggttg ttatcgctga
    17281 acagagccta gaagattaga aatggcatca agtaagaact atttggaatt tgttttggaa
    17341 caattatcag gattagatga tgtgacttac cgttccatga tgggggagta tattctttac
    17401 ttccgcggca agattattgg cggcatttat gacgatcgct ttttagttaa acccgtgcaa
    17461 gcagtcttag ataagattga ccaatcttct tttgagtttc catacaaagg tgccaaagaa
    17521 atgatttgag tggaagaact tgataataag atgtttctat aagacctgat tttagctatg
    17581 tataaccaac tgccaacgcc caaacctaaa aagaaaaagc aagggtgaac gaagtaaaaa
    17641 agaagtctgc taaggccctg tctttgcacg ggtaaaattt tatatataaa aagaagctgg
    17701 gactaaagag ctcagcttcc tttggtttat ataattgtca ttacaagacg aagtggttgg
    17761 gcgaaactct gttgatttta ttcaatttag agtttcttat gcacaattga gtctggaacg
    17821 aaagtctcca gttgcaaagt atacagtaca ataaaccaac gatgtaatag ctgatgacac
    17881 aaagcacagt gggtaggact tgcgaagtca cccttttctt ttcaaaattt atactaaatc
    17941 attgatatca gtgtagtcac gattaagtcc ttgagcaact ggtaggctag ttaagtaacc
    18001 ttgataagtg gtcacacctt gacgcaagcc ttcatcttca gagattgctt gtgcgaatcc
    18061 tttgccagcc aaagcttcga tataaggaag agtgacattg gttagggcga tggttgaagt
    18121 gcgagcaacc gcaccaggga tattggcaac ggcatagtgg agaacaccgt gtttttcata
    18181 gacgggttca tcgtgcgttg tcacacggtc agctgtttcg ataacgccac cttggtcaac
    18241 agcaacgtca acgatacaga gcctggacgc atttgtttga ccatctcatc tgtcaccaat
    18301 tccggtgctt ttgcaccagg gatgagaatg gctccaatca ccacatcagc atctctcaca
    18361 cttgcttcaa tgttgaatga attagatata agagtttgaa tttgacttcc aaagacttct
    18421 tctagaactg agagacgctt ggcactaata tctaaaatag tcacttgagc accaagacca
    18481 agggcgatgc gggcagcatg tgtaccgacg acaccatcac cgatgatagt tacttttcct
    18541 tttggaacac ctggtacacc accaggtaga acaccagagc caccagcttg cttagtaagg
    18601 aagtgagctc cgatttaaac agccatacga cctgcaacct cactcatagg aacgaggagc
    18661 ggtagttgtc cttgattgtc acgaacagtt tcataggcaa ttcctgttgt ttttgctgct
    18721 aacatagcat ctgctaattc tggagcagcg gccatgtgca agtaggtgaa gagaagaaga
    18781 tcgtcgcgca agtaaccgta ttcagaactt aaaggttctt ttactttcac aaccaactct
    18841 gctgcccaag cttcaccagc agtagcgaca atctcagctc cttgcttttg atagtcagca
    18901 tcagtaaagc cagaaccgag accagcattt gtttcgataa ggacacgatg accacgacta
    18961 actaagctat gaacacctgc aggtgtgagg gcgacacggt tttcgttatt tttaatttct
    19021 tttgggattc cgattaacat tgagataacc tacctttcaa ttgacggtct tgttttggtt
    19081 gtcacattcc agttcataaa tcaaaaatgt gacggtttca ttgtatatga aaccgcttca
    19141 aaaatcaaga aaaacttgtc atccaaattt ttttatgcta gactagtgaa aatcaagctc
    19201 taatggaggg aaaagtatgg aatcaatttt tgtaaaactt gcccagtatc cgtctataga
    19261 aacggagcgt ttattgctta gacctgtaac tttggatgat gcggaagcaa tgtttgacta
    19321 tgcctcggac aagggtaata cacgttacac ttttccaacc aatcaaagct tggaagaaac
    19381 caagaataac attgctcagt tctatttggt taatcccttg ggacgttggg gaatagaact
    19441 aaaaagcaat ggtcagttta ttggaaccat tgacttgcac aagattgatt ctgttcttaa
    19501 gaaggcagct attggctaca ttagcaataa aaagtattgg aatcaaggat taacgacaga
    19561 agccaatcgt gctgtgattg agctagcttt tgagaagata gggatgaata agttgactgc
    19621 ccttcacgat aaggctaatc ccgcgtcagg aaaggtcatg gagaaatcag gcatgcgttt
    19681 ttcccatgca gaaccatatg cttgtatgga ccagcatgaa aaaggccgaa tcgtgacaag
    19741 agttcattat gtcttgacca aggaagacta ttttgcaaat aaataagcag ttgaaaagaa
    19801 atttttcgac tgttttttct tcctcttacg aataatctaa gagaggagaa aatatggaag
    19861 caattatcga gaaaatcaaa gagtataaaa tcatcgtcat ctgtactggt ctgggcttgc
    19921 ttgtaggcgg atttttcctg ctaaagccag ctccacaaac acctgtaaag gaaacgaatt
    19981 tgcaggttga agttgcagct gtttccaagg atttggtatc cgaaaaggaa gtgaacaagg
    20041 aagagaagga agaaccagtt gaacaagatc taatcacagt agatgtcaaa ggtgctgtca
    20101 aatcgccagg gatttatgac ttgcctgtag gtagtcgagt caatgatgct gttcagaagg
    20161 ctggtggctt gacagagcaa gcagacagca agtcgctcaa tctagctcag aaagttagtg
    20221 atgaggctct ggtttacgtt cctactaagg gagaagaagc agttagtcaa cagactggtt
    20281 cggggacagc ttcttcaaca agcaaggaaa agaaggtcaa tctcaacaag gccagtctgg
    20341 aagaactcaa gcaggtcaag ggactgggag gaaaacgagc tcaggacatt atcgaccatc
    20401 gtgaggcaaa tggcaagttc aagtcagtag acgagctcaa gaaggtctct ggcattggtg
    20461 gcaaaacaat agaaaagctt aaagactatg ttacagtgga ttaagaattt ctctattccc
    20521 ctaatttacc tgagttttct gttattatgg ctttactacg ccattttctc agtatcctat
    20581 tttgctttgt tgggttttgt ttttctgcta gtctgcctct ttatccaatt tccttggaaa
    20641 tcagcaggta aagttctagt gatttgtgga gtctttggct tctggtttct gtttcaaact
    20701 tggcaacaga gtcaagtgag tcaaaatttg gtggattctg ttgaaagggt acggatttta
    20761 ccagacacta ttaaggtcaa cggtgacagt ctgtcctttc gtggtaagtc tgatggtcgt
    20821 gctttccaag tctattataa actccagtcc gaggaggaga aagaagcctt tcaagcttta
    20881 actgaccttc atgagatagg actagaaggg aagctttcgg agccagaagg gcagagaaat
    20941 tttggtggct ttgactacca aggctatctg aagactcagg gaatttacca gacactcaat
    21001 attaaaagaa tccagtcact ccaaaaggtt ggcagttggg atataggtga aaacctgtcc
    21061 agtttacgtc gaaaggctgt ggtttggatt aagatgcact tcccagaccc tatgcgcaat
    21121 tacatgacag gactcttgct aggacatctg gacaccgatt ttgaggagat gaatgagctt
    21181 tattccagtt taggaattat tcaccttttt gccttgtcag gtatgcaggt agggtttttc
    21241 atggacggat ttaagaaact acttttacga ttgggcttga cacaagaaaa gttgaagtgg
    21301 ctgacttatc ccttttccct tatctatgcg ggactaactg gattttcagc atcggttatt
    21361 cgcagtctct tgcaaaagct actggctcaa catggggtta agggcttgga taattttgcc
    21421 ttgacggtgc ttgtcctctt tattgtcatg ccaaactttt tcttgacagc aggaggagtc
    21481 ttgtcctgtg cttatgcttt tatcctgacc atgaccagca aagaagggga ggggctcaag
    21541 gctgttacta gtgaaagtct agtcatctcc ttgggcatat tgcccattct atccttctat
    21601 tttgcggaat ttcaaccttg gtctatcctt ttgacctttg tcttttcctt tctttttgac
    21661 ttggtcttct taccgctctt gtctatctta tttgtccttt cctttctcta tccagtcatt
    21721 cagctgaact ttatctttga atggttagag ggcattattc gcttggtctc gcaggtggca
    21781 agtagaccgc ttgtctttgg acaacccaat gaatggcttt taatcctatt gttaatttct
    21841 ttggctttgg tctatgattt gaggaaaaac attaaaaagc taacggtatt gtgcttattg
    21901 attacagggc tctttctcct gaccaagcat ccactggaaa atgaaatcac catgctggat
    21961 gtggggcaag gagaaagtat tttcctacgg gatgtaactg ggaaaaccat tctcatagat
    22021 gtaggtggta aggcagaatc ttataagaaa atcaaaaaat ggcaagaaaa gatgacgacc
    22081 agcaatgccc agcgaacctt gattccctat ctcaaaagtc gaggagtagc taagattgac
    22141 cagctaattt tgactaacac ggacaaggag catgttggag atttgtcaga gatgaccaag
    22201 gctttccatg taggggagat tctagtatca aaagacagtc tgaaacagaa ggaatttgtg
    22261 gcagaactac aggcgactca aacaaaggtg cgtagtatga tagtagggga gaacttgccc
    22321 atttttggaa gtcagttaga agttctatct ccaaggaaaa tgggagatgg aggacacgat
    22381 gataccctag ttctgtatgg gaaattcttg gataagcaat ttctcttcac gggaaatttg
    22441 gaggagaaag gagagaagga cttgctgaag cactatccag acttgaaagt aaatgttttg
    22501 aaagctagcc aacatggcaa taaaaaatca tcaagtccag cctttctaga aaaactcaaa
    22561 ccagagctta ctcttatctc agttggaaag agcaatcgaa tgaaactccc ccatcaggaa
    22621 acattgacac gactggaagg tatcaatagc aaagtttatc gaactgacca gcaaggagct
    22681 atacgtttta aggggttgga tagttggaaa atcgaaagtg ttcgatagga aggataaatg
    22741 ttgtagatta gtgaaataaa ctaaaaattt gttgcataat aatgataaaa atggtataat
    22801 gaaaacgtat tcaatattga ggatataaaa tcattaaaaa tcagcaaaag ttgttttatt
    22861 agttagttta taatctattg gtcttcttca gtccagtgta tctgctgtga cagccactaa
    22921 aagttacaag tatgattgga atacggtttt ggaatatagt accaactatc acgaccatca
    22981 gtatgcttgg attccggaat ggtctcgtta ttacagctat tctgagtata aagttggcgg
    23041 aggctggaac tacgctcgtt atgaggtcat aaactactat actggaggtt attaatcctt
    23101 aaagagtgag aaaaggaggg ctggatatgt taaatcttac tcatgttacc ttaaaaacgc
    23161 gacaagtcat cttgcaagat gcggatttta cctttaaaaa gggtaggatt tatggccttc
    23221 ttgctatcaa tggctcggga aagacgacac tattccgagc tatgagcaag ttgcttcccc
    23281 ttagtagtgg acacatcgca gttcctcctt ctttgtttta ttatgagagc gttgaatggc
    23341 tggatggaaa cttaagtggg atggactacc ttcgtctcat aaaaaacatc tggaagtcag
    23401 acctaaactt gagagatgaa atcgcctact gggaaatggc tgactatatc agtcttccca
    23461 tccgcaagta ttccttaggg atgaagcaac gcttggtgat tgctatgtat tttctcagtc
    23521 aggccaaatg ctggctcatg gatgagatta caaatggctt agacgagtat tatcgacaga
    23581 agttttttga taggctagca caaatcgata gacaagaaca gctggttctt ttaagttccc
    23641 actataagga agagttggtt gatatctgcg atagagtagt aaccattcat caggggcaga
    23701 tagaagaggt ttagtttatg aaagatgtta gtctattttt attgaaaaaa gttttcaaaa
    23761 gtcgtttaaa ctggattatc ttacttttat ttgcatctgt actcggtgtt accttttatt
    23821 taaatagtca gactgcaaac tcacacagct tggagagcag gttggaaagt cgcattgcag
    23881 ccaacgagag ggctatcaat gaaaatgaag agaaactctc ccaaatgtct gataccagct
    23941 cggaggaata ccagtttgct aaaaataatt tagacgtgca aaaaaatctt ttgacgcgaa
    24001 agacagaaat tctaacttta ctaaaagaag ggcgctggaa agaagcctac tatctgcagt
    24061 ggcaagatga agagaagaat tatgaatttg tatcaaatga cccgactgct agctctggct
    24121 taaaaatggg ggttgaccgc gaacggaaga tttaccaagc cctgtatccc ttgaacataa
    24181 aagcacatac tttggagttt ccgacccacg ggattgatca gattgtctgg attttagagg
    24241 ttatcatccc aagcttgttt gtggttgcga ttatttttat gctaacacaa ctatttgcag
    24301 aaagatatca aaatcatctg gacacagctc acttatatcc tgtttcaaaa gtgacatttg
    24361 caatatcctc tcttggagtt ggagtgggat atgtaactgt gctgtttatc ggaatctgtg
    24421 gcttttcttt tctagtggga agtctgataa gtggttttgg acagttagat tatccctacc
    24481 caatttatag cttagtgaat caagaagtaa ctattgggaa aatacaagat gtattatttc
    24541 ctggcttgct cttagctttc ttagccttta tcgtcattgt ggaagttgtg tacttgattg
    24601 cttacttttt caagcaaaaa atgcctgtcc tctttctttc actcattggg attgttggct
    24661 tattgtttgg catccaaaca attcagcctc ttcaaaggat tgcacatctg attcccttta
    24721 cttacttgcg ttcagtggag attttatctg gaagattatc taagcagatt gataatgtcg
    24781 atctaaattg gagcatgggg atggtcttac ttccttgcct gattatcctt ttgttagtgg
    24841 ggattctatt tatcgaaagc tggggaagtt cacgtaaaaa ggaagttttt aaagtttagt
    24901 tttcctatag ggaagctaaa gaaaaactaa catagggaga gaatcaactt ggttctctct
    24961 ttttgattcg aaaaccaaac caaaatacaa acacaaactt ttcaaaaaat aactttttat
    25021 cttgacaaga gctagaaaac ttggtatcat gtaaaagttg agaaaagcag aagtgagagc
    25081 ttctcgcctt gtgacattaa gttgcctggc cctacggatg aaaagtttcg aagaaacgct
    25141 atcataacgt gcgggcttgt atatttacaa gtccgctatt gtttttctct aataaaacaa
    25201 aagaggtgaa aaccatagca aagcaagact tattcatcaa tgatgagatt cgtgtacgtg
    25261 aagttcgctt gattggtctt gagggagaac agctaggcat caagccactc agtgaagcgc
    25321 aagctttggc tgataacgct aatgttgacc tagtattgat tcaaccccaa gccaaaccgc
    25381 ctgttgcaaa aattatggac tacggtaagt tcaaatttga gtaccagaag aagcaaaaag
    25441 aacaacgtaa aaaacaaagc gttgttactg tgaaagaagt tcgtctaagt ccaacaattg
    25501 acaagggtga ctttgataca aaacttcgta atgcacgtaa gttccttgaa aaaggaaata
    25561 aagttaaggt atctattcgc tttaagggtc gtatgattac ccataaagag attggtgcaa
    25621 aagttttagc cgagtttgct gaagcaactc aagatattgc catcatcgaa caacgtgcta
    25681 aaatggatgg acgtcaaatg ttcatgcaat tggcgccagc gactgacaaa aaataattgt
    25741 cagaaagtta aataaaggag aaaaaatcat gccaaaacaa aaaacacacc gcgcatcagc
    25801 taaacgtttc aaacgtacag gttctggtgg acttaaacgt ttccgtgctt acacttctca
    25861 ccgtttccac ggaaaaacta agaaacaacg tcgtcatctt cgtaaagcat ctatggtgca
    25921 ttcaggagat tacaaacgta tcaaagcaat gcttactcgc ttgaaataat agcttgactg
    25981 taagtaaaca attctaggaa atatttggag gaaataaaac atggcacgtg ttaaaggtgg
    26041 cgttgtatca cgcaaacgtc gtaaacgtat tcttaaatta gcaaaaggtt actatggagc
    26101 taaacacatc ttgttccgta ctgcaaaaga acaagtaatg aactcttact actatgcata
    26161 ccgtgaccgt cgtcagaaaa aacgtgactt ccgcaaattg tggattactc gtatcaatgc
    26221 ggcagctcgt atgaacggac tttcatactc acaattgatg catggtttga aattggctga
    26281 gattgaagtt aaccgtaaaa tgcttgctga cttggctgtt aacgatgcag tagctttcac
    26341 agctcttgca gatgcagcta aagcaaaact tggtaaataa taactgatga gactggaaca
    26401 ggattgtcca gtctttttaa aaataaagga gaaagatatg gcttcaaaaa tgctacacac
    26461 ttgcttacga gtagaaaatc ttgaaaaatc aatcgcattt tatcaagatg cttttggttt
    26521 taaagaattg cgtcgcagag attttccaga ccatgccttc acgattgtct atctaggtct
    26581 tgagggtgat gattatgagt tggagttgac ttataactac gatcacggtc cgtatgtggt
    26641 tggtgatggg tttgcccata tcgccctcag tacacctgat cttgaggcac ttcatcaaga
    26701 acacagtgca aaaggctatg aagtgactga gccaaatggt ctaccaggaa ctacaccaaa
    26761 ctattacttt gttaaagacc ctgatggata taaggtcgaa gtcattcgtg aaaaataatt
    26821 tcgtgcaagt taagtagaat attcgttttc tacttgactt tttttagctg aaagagtata
    26881 ctaataaaaa ttcaaccttt aaggggagtc cagagagact cacaaggtgt cagataaaag
    26941 aatagtgcaa ttttctagag gagacctttt gagtgtgctc tcttgtgttg tacgatttta
    27001 actgaggcct tgcactagca aggtcttttc tttatctggt ccccttaaaa tttaaggagg
    27061 aaaagttatg aatctcacat gtaagaagcg tttgggtgtc attcggttgg aaaccatgaa
    27121 ggtggttgca caagaggaaa tcgcgccagc aatctttgaa ttagtcctag aaggagaaat
    27181 ggttgaagcc atgcgagcag gccaatttct tcatctgcgt gtaccagacg atgcccatct
    27241 cttacgtcgt cctatttcaa tttcgtctat tgacaaggca aacaagcagt gtcacctcat
    27301 ttatcggatt gagggagctg ggactgcaat tttttcaacc ttaagtcagg gagacactct
    27361 tgatgtgatg gggcctcagg gaaatggttt tgacttgtct gaccttgatg agcagaatca
    27421 ggttctcctt gttggtggtg ggattggtgt tccacccttg cttgaggtgg ccaaggaatt
    27481 gcatgaacgt ggagtgaaag tagtgacagt cctcggtttt gctaataagg atgctgttat
    27541 tttgaaaacg gaattggctc agtatggtca ggtctttgta acgacagatg atggttctta
    27601 tggcatcaag ggaaatgttt ccgttgttat caatgattta gatagtcagt ttgatgctgt
    27661 ttactcgtgt ggggctccag gaatgatgaa gtatatcaat caaacctttg atgatcaccc
    27721 aagagcctat ttatctctgg aatctcgtat ggcttgtggg atgggagctt gctatgcctg
    27781 tgttctaaaa gtaccagaaa gcgagacggt cagccaacgc gtctgtgaag atggtcctgt
    27841 tttccgcaca ggaacagttg tattataagg agaaaattat gactacaaat cgattacaag
    27901 tgtctctacc tggtttggat ttgaaaaatc cgattattcc agcatcaggc tgttttggct
    27961 ttggacaaga gtatgccaag tactatgatt tagacctttt aggttctatt atgatcaagg
    28021 cgacaaccct tgaaccacgt tttgggaatc caactccaag agtggcagag acgcctgctg
    28081 gtatgctcaa tgcaattggc ttgcaaaatc ctggtttaga ggttgttttg gctgaaaagc
    28141 taccttggct ggaaagagaa tatccaaatc ttcctattat tgccaatgta gctggttttt
    28201 caaaacaaga gtatgcagct gtttctcatg ggatttccaa ggcaactaat gtaaaagcta
    28261 tcgagctcaa tatttcttgt cccaatgttg accactgtaa tcatggactt ttgattggtc
    28321 aagatccaga tttggcttat gatgtggtga aagcagctgt ggaagcctca gaagtgccag
    28381 tttatgtcaa attaaccccg agtgtgaccg atatcgttac tgtcgcaaaa gctgcagaag
    28441 atgcgggagc aagtggcttg accatgatca atactctggt tggaatgcgc tttgacctca
    28501 aaaccagaaa accaatcttg gccaatggaa caggtggaat gtcaggtcca gcagtctttc
    28561 cagtagccct caaactcatc cgccaagtag cccaaacaac agacctgcct atcattggaa
    28621 tggggggagt ggattcggct gaagctgccc tagaaatgta tctggctggg gcatctgcta
    28681 tcggagttgg aacagctaac tttaccaatc cttatgcctg tcttgacatc atcgaaaatt
    28741 taccaaaagt catggataaa tacggtatta gcagtctgga agaactccgt caggaagtaa
    28801 aagagtctct gaggtaaatt gcaatcaatc tgttcttgat tttttattag tttgtaatat
    28861 gaatttagga g
//
LOCUS       DAVGJJ010000016        27225 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000016 DAVGJJ010000000
VERSION     DAVGJJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.016"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(181..1017)
                     /locus_tag="VVE35_000657"
     CDS             complement(181..1017)
                     /locus_tag="VVE35_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3060896.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1030..1659)
                     /locus_tag="VVE35_000658"
     CDS             complement(1030..1659)
                     /locus_tag="VVE35_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3060897.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(1669..2310)
                     /locus_tag="VVE35_000659"
     CDS             complement(1669..2310)
                     /locus_tag="VVE35_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3060898.1"
                     /translation="MSYLFEVLPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYNYYR"
     gene            complement(2456..3685)
                     /locus_tag="VVE35_000660"
     CDS             complement(2456..3685)
                     /locus_tag="VVE35_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU3060899.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KANLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(3675..4841)
                     /locus_tag="VVE35_000661"
     CDS             complement(3675..4841)
                     /locus_tag="VVE35_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU3060900.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            4985..5998
                     /locus_tag="VVE35_000662"
     CDS             4985..5998
                     /locus_tag="VVE35_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU3060901.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVCMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6043..6462)
                     /locus_tag="VVE35_000663"
     CDS             complement(6043..6462)
                     /locus_tag="VVE35_000663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU3060902.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6473..7879)
                     /gene="atpD"
                     /locus_tag="VVE35_000664"
     CDS             complement(6473..7879)
                     /gene="atpD"
                     /locus_tag="VVE35_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU3060903.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(7965..8843)
                     /locus_tag="VVE35_000665"
     CDS             complement(7965..8843)
                     /locus_tag="VVE35_000665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU3060904.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNDLFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(8859..10364)
                     /locus_tag="VVE35_000666"
     CDS             complement(8859..10364)
                     /locus_tag="VVE35_000666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU3060905.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(10379..10915)
                     /locus_tag="VVE35_000667"
     CDS             complement(10379..10915)
                     /locus_tag="VVE35_000667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU3060906.1"
                     /translation="MDKKTVKVIEKYSIPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(10915..11409)
                     /gene="atpF"
                     /locus_tag="VVE35_000668"
     CDS             complement(10915..11409)
                     /gene="atpF"
                     /locus_tag="VVE35_000668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU3060907.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(11423..12139)
                     /gene="atpB"
                     /locus_tag="VVE35_000669"
     CDS             complement(11423..12139)
                     /gene="atpB"
                     /locus_tag="VVE35_000669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU3060908.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12174..12374)
                     /locus_tag="VVE35_000670"
     CDS             complement(12174..12374)
                     /locus_tag="VVE35_000670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU3060909.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(12449..13456)
                     /locus_tag="VVE35_000671"
     CDS             complement(12449..13456)
                     /locus_tag="VVE35_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU3060910.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKTSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPIEDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            complement(13876..14274)
                     /locus_tag="VVE35_000672"
     CDS             complement(13876..14274)
                     /locus_tag="VVE35_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060911.1"
                     /translation="MENKESYKSQEIGNLIRAYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEQLRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAL
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQRNL"
     gene            14402..14479
                     /locus_tag="VVE35_000673"
                     /pseudo
     CDS             14402..14479
                     /locus_tag="VVE35_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(14602..15084)
                     /gene="greA"
                     /locus_tag="VVE35_000674"
     CDS             complement(14602..15084)
                     /gene="greA"
                     /locus_tag="VVE35_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU3060912.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(15155..16810)
                     /gene="mltG"
                     /locus_tag="VVE35_000675"
     CDS             complement(15155..16810)
                     /gene="mltG"
                     /locus_tag="VVE35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU3060913.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(16891..17385)
                     /locus_tag="VVE35_000676"
     CDS             complement(16891..17385)
                     /locus_tag="VVE35_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060914.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(17382..17846)
                     /locus_tag="VVE35_000677"
     CDS             complement(17382..17846)
                     /locus_tag="VVE35_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060915.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(17856..19190)
                     /locus_tag="VVE35_000678"
     CDS             complement(17856..19190)
                     /locus_tag="VVE35_000678"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU3060916.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQRY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(19201..19818)
                     /locus_tag="VVE35_000679"
     CDS             complement(19201..19818)
                     /locus_tag="VVE35_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU3060917.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     GFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRVTE"
     gene            complement(19866..22964)
                     /locus_tag="VVE35_000680"
     CDS             complement(19866..22964)
                     /locus_tag="VVE35_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3060918.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDQILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEV
                     ST"
     gene            complement(23057..24223)
                     /locus_tag="VVE35_000681"
     CDS             complement(23057..24223)
                     /locus_tag="VVE35_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3060919.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFDRSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(24231..25325)
                     /locus_tag="VVE35_000682"
     CDS             complement(24231..25325)
                     /locus_tag="VVE35_000682"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU3060920.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(25483..27165)
                     /locus_tag="VVE35_000683"
                     /pseudo
     CDS             complement(25483..27165)
                     /locus_tag="VVE35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         8015 a         6052 c         4948 g         8210 t
ORIGIN      
        1 agtatttcat tacagaatat tttaattttg gaagtcaaat caatttttaa caatgtttta
       61 gaaatcacag tgggctattc catattcaat tatccagatt caatcgctat tatcgttttc
      121 ctagattaca aaaaaactac gttttgctaa tcaaaacgca gtttcagcca ctattttatc
      181 ttactgtcct tcttttactt ctttggttgc tacatcttct ccaaaccatt tttggctgat
      241 ttcttggaac ttgccgtcct tgtaaagact agaaaaagct tcatttatct tcttaaccaa
      301 ggttgtatct tccttacggg ctccaaccgc aaaagcttct gtttctagtc caactgtaaa
      361 gacattataa tcgtttaaaa caccttctgc ttctaaataa tagtttgcat agacacggtc
      421 aatcaataga ccatcaattc gatcgttttt caaatcaatc aaggcttcat taaaggtttg
      481 gtattgattc gcttccttat tagcgacaat attcttcaaa atttctggat ttgcttcaaa
      541 gtccgcataa ccagatgaac cagcttgagc tcctaatgtc tttccagtca tatcctttgc
      601 agtcgtgata ccagatgatt tcttcgtaac caatacctgc tcattcttca tatatgagtt
      661 actgaaagcc accttttcac ggcgttcgtc tgtagcggaa tagccattcc aaatcagatc
      721 aatcgttcct tttgtcaatt cagcttcttt caaatcccaa tcaatcggtt gccaatttac
      781 cgtgattccg tatttttcaa aaacagctgt agctaaatca atatcaaatc ctgcataaga
      841 accatctttc tgagcaaatc ccattggaac aaaagtacta tcaaatccaa tagtaataga
      901 cttgttagac tggtactttg accaattatc tccactagtt tcgctagaat ttttcccaca
      961 agctactaag aacaaagcag tcatcagact gactaataca agcatccatt ttttcatcca
     1021 tttttcctcc tatttaggtt ctactttcaa taatacatct gcgatatttt cagcaaactg
     1081 caaatcatgg gtaaccacaa tctgggtcat cccaagttcc ctattttgca agattagctt
     1141 ctccacttcc aaacgtaatt ctggatccag ggcagaagtt ggttcatcgt agccaatgat
     1201 ttctgggtca atcatcatag cacgcgccaa agccacccgc tgcttttgcc caccagatag
     1261 tgagaaagga taggcctctg cgtgtcctcc tagtcctaac tgttccaaga gttcactcgc
     1321 cttcttctca gcctcttcct gcttcattcc catggtcttc acaggcgata aagtcaaatt
     1381 ttccagaact gatagatgag gaaatagttg aaaatcttgg aagacaaatc ccagtaggtt
     1441 gcgcttctgc aattcatcta gctctaaagg ttgtccatta taaaagattt gccctgaatc
     1501 aatggtttca agacctgcaa gcatacgtaa aagagttgtc ttacctccac cagaaggtcc
     1561 aacgatagcc aggatttgct tttcaggaat acttagactg aaatcagaca ggatttgttt
     1621 gtctccaaag actttattga tatttcgtaa ttctaacata gctgccccct atctataata
     1681 attgtacttt ttctcaactt ttttggaaat aattgtcaca atcccaatta aaatcaagta
     1741 aatggctcct gccaagaaca taggaaccag actagcatcg cggttagcag ctgttcgact
     1801 agccaagata aggtctgaaa ttccgagagc atagaccaaa gaagtatcct tgaccaaact
     1861 cataacttca ttaaagacac taggaagaac gatcttggtc acttggggca agataatata
     1921 gcgcactctg tcaaaagggc taaacttcaa gaccttggca gcctcatact gtcctcttgg
     1981 aatagtgtca attcccccac ggaaaatttc tgcaaagtaa gctgcatagt tgagaacaaa
     2041 ggcaataata gctgcaggaa ggcggtctaa acgaatccca atacttggga gcacataata
     2101 gataaaaatc agttgcaaga gtaagggggt tcctcgcata acccagatgt aaatgttaat
     2161 cagataatgg aggggcttcc aatggacttg caaggcaaag gcaatcaaaa cgcccaaggg
     2221 aatcgaaaat agcaagacca gtgcaaagac ctgtacagtc gtgctcgcac cattcagtaa
     2281 actcggtaat acctcaaata aataagacat actgcacctc ctaaaagtaa ttgttcctat
     2341 tatagcatga ataaacaaga ttaccaagta tttttgtaaa aagattctac catatacaaa
     2401 taaaaaccgt agctatcaaa agagataaac tacagttcta tatgtttaaa cattcttaca
     2461 atctctcaaa cagttcttgc atttgcacat catctggaac cagttttaag taagtgtgag
     2521 catggacttt tgcttcttca aaatgtccca attcacgcaa gagatagata tagtgttcca
     2581 gaaattctgg attgtccttc aaatctcctg tcaactcttg ataatgctca taagcagtat
     2641 ccaaatcgtc catttcttga taagaacgag caatcatcca cttggtcaaa agattttctg
     2701 gctcctcact ctgcaagtct agaatatcct cataacgctc ctgctccaga taaatagtgg
     2761 ctaaacgaag caagatttct tctgtatcct cagcgtcttc ttttgcagta aggagataat
     2821 tttctgcacc actagcatca tgcaattcat aagaaaattg tgaagcagct agcaagaggc
     2881 gagtttcaaa gggatttttc tctaatcctt gcttagcgat acgcagggct tcttgaactt
     2941 gatgttcctt atgtaaagcc tgactgtacc catactcata gccttcaaag tcaggagaaa
     3001 tggtatcaag ctgcttgaag tagagggtgg ctttttgata ttcttcttga tcaaaataaa
     3061 gactggccaa ctcaaaagct gttaagtcat cgtattctaa ctccagggct ttttctaaaa
     3121 actcagtagc cgtttcaaat ttccctaact gagcataggc aaagccaatt cgttgatagg
     3181 tggaaatgcc cgtttgctca taaatcgagc gattatctaa ctgggcatag gcttgaatag
     3241 ccgcttggta attttccaac tcactatcca actctgccaa acccaatatc aagagagaat
     3301 cctctgagta ggtcaaggcc tccaataatt tctcacgtgc cacatctgtc aaaccttcca
     3361 gctggtaaag gtttgccttc agagccaaag acgagacata ccagtcactg tcagcttgga
     3421 tttcctcaag ataggtaaag gcttcttcta tttgaccatc ctcgctagca attgcagcta
     3481 gattaagatg aacctctgga aaatcctcta caattttcag gtaaatttcc ttggcctgag
     3541 gatagaaacc aatcccttca agataagttg ccaactcata cagaagatca cttgaatcat
     3601 tttctaaagc tttggcgaaa taatgctcag ccttagttaa atcttgctcc tccaaagcct
     3661 gtaacatctg ttgactattg ttcactcttg acttcctcac cctctaattc atatagacca
     3721 ctgactacct tataccattc gaaaatggct gaaatgacaa ccttagcaga ggcataaacc
     3781 ggaataccaa gtaaaactcc ccagatacca aacatagatc ctgaagttaa caaaacaaag
     3841 agaacattaa tagggtggat gtttaattga cttcccaaaa tcaatggaga gacaaaacgg
     3901 ccttcaatag tttgttctac aataaagaca atcactactt tcaaaagcat gactggacca
     3961 gcaatcaaac ccaatactag ggcaggaagc atggctagaa agctaccaag ataagggacc
     4021 agatttaaaa taccagcagt aacccccagc gtaaccgcat agcgtagacc aataatcttg
     4081 aagaagatga taaacattac tgctacaata atagccactg tcacttgccc tcgaacatag
     4141 ttggacaact gttgattcac atctgataga acttgtccaa caggttcctt caattttctt
     4201 ggaatgaatt gggtcaaata gttacgcaag cctttcccat cacgcaagag ataaaagagc
     4261 atgaaaggaa cgataatcaa ggcaacaatc acttgagaag ctccgctaat aaaggcactc
     4321 acccagttga ctgcctgaga tgaaacctta cttgccaaaa ctgtagcctg gctagaaaaa
     4381 ttggtcaaaa cttgctctaa ttgaggtctg aaatcatctg gcaggtgctg agctaccaat
     4441 ccattaacaa tcctatctat atcttctaag taaacaggaa cgtttcttgc aaaggtcaaa
     4501 acctgacgtt gcagatttgg aatggcgact gccaagcccc aaatgataaa gagagcgatg
     4561 ataacaaaga caatagtgat agctataaca cgattaacct tatgcttctc catccaatca
     4621 acaataggat tcaacaaata atataacaaa ccagacaaaa tgactggcaa catcacaact
     4681 gctaaaaagt ctaaaacagg tgaaaataga aaactaatct tacttaaaat aaaaagattc
     4741 aatcccaata ataaggttac taaaaatacc gtaattgcct tgttatctaa aaaccacttg
     4801 aaaaaccaag ataggctaaa atgtttctct ttttgctcca taaatacttc ctttctattg
     4861 ttctctcatt ataccatttt tgaaacaaaa taactctttt ttaaagtgct tacatctaat
     4921 tggatatctt taatttactt gtttttgtga tataatagtc ttatctttac acagaggtat
     4981 agtaatgaaa gaaactgttt attttggaac ttatacacgt cgtacttctc aagggattta
     5041 caaggcagac tttgatacag aaactggtca gctttcaaat ctagaacttt ttgcagctga
     5101 gccaagtcca acctaccttg cctttgacca gcaccaacat ttatacactg ttggtagcca
     5161 agacgataag gggggaattg cagcctatca aactgacggg actgtgttaa atcatgttgt
     5221 tgaagaagga gctccccact gttatgttgc tgttgatgaa aagcgtgatt tggtttacgc
     5281 agccaactat cacaagggac aagtccttgt ttataaacgc caggaagatg gtagtcttct
     5341 acttagtgat atggatcaac acagtggcca aggtccacat gaaaatcaag cttcccccca
     5401 tgttcactat acagatttaa cacctgacca ctatctagtg acctgcgact tgggaactga
     5461 ccaagtcatc acctatgacc tcgatcaaga aggaaaatta tctaagctct atacctatca
     5521 cagcaagcca ggagcaggct cacgccatat cattttccat aaccactata aaatcgctta
     5581 tctcatttgt gaactcaata gtactatcga agttttaatc tacgatggcg ttggcgaatt
     5641 tgtatgtatg caggttattt caactttacc agaagcttac gaaggcttta atggaaccgc
     5701 tgctattcat ctctctaaag acggtaaata cctctacgct tctaaccgtg gccatgattc
     5761 tatcgcagta tataccatcc ttgcggacgg tagcttagag ttattagaaa tcgttccaac
     5821 gcatggtcag actccacgtg attttgattt gacacccgac caaaaatttc tcattgttgt
     5881 ccatcaagac tctgacaatg caactgtctt taaacgtaat tgtgacaatg gtcgtctagc
     5941 agaactctcc aacgacttcc atgttcccga agcagtctgc atccgttttg ctccttaaaa
     6001 caccataaaa aatgaacttg gtattttcaa gttcattttt tcttatagtc tatttccgac
     6061 attaatacgg ttaatggcac gttgcaaagc aatcttagca cgacgttctt ggtcaatcaa
     6121 atgtttgtct tgtgcttctt caattgcacg ttctgcacga agtttggcac gttctgcacg
     6181 actgatatcg atatcacgag cacgttctgc agagtcagcg acgattgtga tcatatcatt
     6241 ggcaatttca ataacgccac cgtttactgc aatccagttc acgtgatctt tatcatcgat
     6301 acgttttacc tttacttcat caactgctaa aaccgcaatc atattttcat gtcgtggcaa
     6361 gatccccatc tcaccatcca gagttcgaac cgatacatag ctggcatggt gatcatagac
     6421 gagaccatct ggtgtcacga tctggacagt taactgagcc ataggtcacc tcttaaaatc
     6481 ccattttttc agcttttgca atcacatctt cgatagaacc tacaccacgg aaggcatctt
     6541 ctggcaagtg gtcgtattta ccatcaagga tttccttaaa gccacgtaca gtttcagcaa
     6601 ctggaacata agaacctggc tgaccagtaa attgttccgc aacgttgaag ttttgtgaca
     6661 agaagaactg gatacgacgg gcgcgagcaa ccaaggtctt ttcttcatca gaaagctcat
     6721 ccataccaag gatagcaatg atatcttgca attcatggta acgttgaaga acacgtttta
     6781 cttcagcagc aactgcatag tgctcttctc caacgatttc aggtgccaag gcacgtgagc
     6841 ttgaagcaag tgggtcaacg gctgggtaga tacccaattg taccaactta cgttccaagt
     6901 ttgttgttga atccaagtga gcgaaggctg ttgctggcgc tgggtcagta tagtcatccg
     6961 ctggcacata gatagcctgg atagaggtta cagaaccctt cttggttgat gtgatacgtt
     7021 cttgcaattg acccatttcc gtagcaagtg ttggttggta accaacggct gatggcatac
     7081 gacccaaaag ggcagatact tctgaaccag cctgagtgaa acggaagata ttatcgataa
     7141 agagaagcac gtcttggcct tccacatcac ggaagtattc agcgattgtc aaaccagtaa
     7201 gggcaacacg catacgtgct cctggtggct cattcatctg accaaagacc atggctgttt
     7261 tctcgataac gcctgattct ttcatttccc agtaaaggtc attcccctca cgagtacgtt
     7321 ccccaacacc agtaaatact gaaataccac cgtgctcttg ggcaatgttg tgaatcaatt
     7381 cttggattaa gacggtttta ccaactccgg caccaccgaa aagtccaact ttaccacctt
     7441 taaggtaagg ggcaagaagg tcaataacct tgatccctgt ttcaaggatt tcagaagagg
     7501 tagacaactc atcaaaagtt ggagcttttt tatgaattgg ctgacgctct gcgtcttctg
     7561 taaaaggagc ttccaaatca atggtatctc ccaaaacgtt gaagacacgt cccaaagttt
     7621 ctttacctac tggtacagag attggacgac ctgtgtccaa tacttccatt ccacgagtca
     7681 acccatctgt tgattccatg gcgatagtac gaaccatacc atctcctaac tccaaggcta
     7741 cttcaaggac gatttttgtt tttctttcgt catttttgta gacgacaagt gcattgttaa
     7801 tctcaggaag tttttcccct gctgcaaaca aaacgtctac aacgggaccg ataacctgag
     7861 caattttacc tgaactcatc tccttctcct attctatata agatactgtc ggttcctagc
     7921 tcaactaggt cctaagttca ttttcatacg agctggacta gagcctattc taaggcacta
     7981 gcacctgcta cgatttctgt aatttcttgt gtaatcgccg cctgtctggc acggttatac
     8041 tgaattgtca aatcattgat gactttctta gcattatctg tcgctgtttg catggctgtc
     8101 atacccgcag cattctcagc tgtcttggca tcgataatgg caccgtaaat catactttct
     8161 gcaaactgag gcaacaactg ctccagaatt tcttctcggc tggtttccaa ttcaaaagtc
     8221 aagctgtact cttcatccgc ttcatttgga tccaagtcaa caatcggaag catttgttcc
     8281 acacgcattt gactggttag cgtattgaca tggtggttgt agcaaacata aagctcatca
     8341 aagaggtcat tttggtacat ttcaacagtt tttgaaataa tcttacgaac ttgatcaaag
     8401 ctaggttggt ctgccaagcc acgtaattca taaagtggtt gaataccgcg agccttaaag
     8461 aaatcagctc ccatcccacc gatacagatc atttcaaaac ctttaccgtc tgggtggtat
     8521 tcttctttca actccataac agctttcaaa atagaggaat tataacctcc aaccaaaccg
     8581 cggtctgaag tgataacgat atagcctgtc ttcttcacag aacggctaat caacatcgga
     8641 ttagttgaag caccagctcc attaccatga aggatatctg tcaaaagttt acgcactttc
     8701 tgagcgtaaa cttggaagtt gcgagcagct tcttcagagc gacctaactt agcagccgat
     8761 accatttgca tggcattagt gatttgactc gtattttttg ttgaggcgat ttttgtttta
     8821 atatcattta gagacactgc catctgacac ctctattctt attggaagct agattgattg
     8881 agaaactctg taatcgcagc atccaagact gcttcttctg gcaagtcttt tgtatcacga
     8941 atggtttcca aaatctctgg atgttgagca tcaaagaagg catggaactc ttcctcgaaa
     9001 cgaacaatat catctactgg aacagtatcc aagaaaccat gtgtcaaagc ataaagaatg
     9061 gttacttgtt tctcaacagg taatggtttg tgaacaggtt gtttcaagac ctcaacggta
     9121 cgacgtccac ggttcaactt agcctgtgtt gctgcgtcca agtcagaacc aaacttagta
     9181 aaggcttcca actcacggta tgaagcaagg tcgatacgaa gtgtaccagc aaccttcttc
     9241 atggctttga tttgtgcaga accacctaca cgagatacag atgaacccgc atcgatggct
     9301 ggacgaatac ctgcattgaa gaggccatcg ccaaggaaga tttgtccatc agtgatagaa
     9361 atcacgttgg ttgcgatata ggctgagata tctcctgctt gtgtctcgat aaatggtagg
     9421 gctgtaattg atccaccacc aagttcatca gaaactttag ctgagcgctc aagcaaacgg
     9481 ctgtggagat agaaaacatc ccctgggaag gcttcacgac ctggaggacg acgaagcaag
     9541 agcgacagtt cacgataagc taccgcttgt tttgatagat catcgtatac aatcaaaaca
     9601 tgcttacctt gatacataaa ttcttccgcc atagcgaccc cagtataagg agctaggaag
     9661 agcaatggag atggttgtga agcagaggct gtcacaacga ttgtgtagtc caaggcaccg
     9721 tactgacgaa gtgtttctac ttgcgtacga actgttgatt ctttttgtcc aatcgcgacg
     9781 tagatacaga tcatatcttg atctttttgg ttcaagattg tatcaatcgc aatggttgtt
     9841 ttccctgtct gacggtcacc gataatcaac tcacgttgac cacgaccaat cggtacaagg
     9901 gcgtcaatag ctttcaaacc agtttgcaat ggttctgaaa cagacttacg ttgcataaca
     9961 ccaggagctg gtgcttctac tggacgagtt ttatcagtgt ggatttctcc aagaccgtca
    10021 actggacgac caagcggatc cacaacacga ccaatcagac tttcacctac agggacttcc
    10081 atgattttcc ctgtacggcg gattgtatcg ccttcacgga tatctgtaaa gtcacctagg
    10141 atgataatac caacgtctgt tgactccaag ttttgagcca taccataaga gccgttttca
    10201 aaattcaaca actctccact catgacattt tcaaggccgt gagcacgcgc gataccgtcc
    10261 ccgatatagg ttacaacacc tgtttcagtc acatcaaaat tgggtttgaa attttcaatt
    10321 tgttgcttaa ttaaagcgct gatttcttgt gcgttaattg ccaaaagaac accactttct
    10381 atttcaaatt ttctttaaca actttaagtt gttgtttaat actcacatca attgtcttgt
    10441 gattggcaaa aatgacaaaa ccaccaatga gactttcatc gatttgttct tttacactcc
    10501 ttactttcag agacattttt ttctcaatca aagggagcaa acgagtcttc tgttcatcag
    10561 ttagaggatg agcagacgta atcgtcactt caaatcgatt tgtttctttt tcaagtcggt
    10621 tcaagcaatc tacaagcaca tcataaaaaa gatttgctct gtgattgtag gccagaacct
    10681 ggataaagtt ttgtaataaa ggtgacacag aatcttggaa aaaagcaatt gttttttcct
    10741 tatccgactc gtctactgcc acttgtttta aaaaagaagg cagacctgtt ttttcaacaa
    10801 cttgcttgat ttgagtcaag tctgaaaaga tacggtcttc ttctcctttt tcaagtacca
    10861 attggacaaa aggtatgctg tatttttcaa ttacctttac tgttttcttg tccattaagc
    10921 ttctcctagc tgatcgatat actgatcaat gagtgcttta tgggcatgac tgtcaaggtt
    10981 ttgtgagatg attttaccag ctaagctgat ggtcaaatct gcgacctcac ccttaacact
    11041 ctgtaaagct tctactttat tttgagcaat ttcttgattg gctttttctt ttaagtgtcc
    11101 tgcttctagt ttagcatctg ctaagatatt agccttactt tgctcagctg tttcctttgc
    11161 attttcaatg attgtcttag cttctttacg gctaccagcc aattcatctt cgcgtttttg
    11221 agccaatact tctgcttttt gacgggcttc ttcagctcta tcaatatctg aagcaatttt
    11281 ttcagctctt tcttcgaaaa tgcctgtaat attagaccat gcaaattttt taatcaagac
    11341 tagcaaaaga ataaaagagc cagtgattaa aataaaatta ccaattaatt cacctactgt
    11401 tacgtgcatc agttactcct ttctattcct cttcaatatt aatcttattc cctaaataca
    11461 cagatgtcaa aagagtaaaa acataagctt ggatgcagga aataaagaca gaaaatgcag
    11521 tccaagccaa attagctcca aaggctactg gataccaata aatagcttgg tgggaaagaa
    11581 gaagtaacaa actcgtcata acctctcctg caaagatatt cccaaaaatc cgcaaagcca
    11641 aagataagaa gtttgtaaat tcttccaaga tattcatcgg tatgacaaaa acaggactca
    11701 taaaagattt tatacttttt ttaaatccac gacgacgaac gctttctata tgtgtcaaca
    11761 aaatgacaag aaaagataag gttaagtcat actgtaaatt agcggttggc gaactccacc
    11821 agttagtccc atcgatcgtt tgaagctttg tcattaagcc aaggttattg gcaatcacca
    11881 tgaaaaggaa taaacaaagg aaaaagagtg agtaatcttt catgtagcgc gaaccaatgt
    11941 taggttctgt aaatccaata acaaagtcat agacatactc aagtacattt tgctttcctt
    12001 tgggtttcaa ggtcatattg cggcttgccc aataaataaa gacaaaaata actcccacaa
    12061 tcaacaaagt catggctaac atagtcagat tgaagataac aggaccaata gagatgattg
    12121 gattaatact ttcttccatc taaaatcctc ccttttcttc tcgttccatg tatttatttg
    12181 ataataaatg agaagacaag agttacaaag aaagttcctt caataaaggc aacacctaaa
    12241 aacatcaaac tacgaaactc agaaagcata tctggttggc gtgctactga tttaaacagt
    12301 ccattcatca ataaaccttc accgacagat acgcccatac aggcaataca taagcctaaa
    12361 aatgttaaat tcataacaaa ttctccttta aaataaaatt tacttactta gtgtagtcct
    12421 aaaatttaaa tttgtcaact tttaaccgct attggaagcg tttaataaaa attatttcca
    12481 ttttgctaaa tttcaagttc aagttagcca tccagaagtc ttctatgggt gacttgtagt
    12541 ccaagcattt tttaggatag ttgttaatcc acttttcgat gaatgcgact tctttgggag
    12601 tcattttctt ggttccctta ggtaaccatc tacgaatgag cctgttgtga ttctcattag
    12661 ttcccctttc ccaagaggca taggggtgcg cataatagat gtgctcctca gaaaatacat
    12721 caaacaagcg attgaattcc gttccattat ctgccgtgat ggaaagaatc ttgtgttgtt
    12781 ttaagatgag ttttagagcc tgattgacca catcagcact tttatttgga atcaatcgga
    12841 tgatctgatg tctactcttt cgatccgtca agacaagcaa gcagtagttt ttcgctctcg
    12901 taagtagaac cgtatcaatc tcataatgcc cattctccaa gcgaagattg atagcttcag
    12961 accgctgttc gatggattga ccagcaggtt taaagttggt gctagcctgt ttcttaagcg
    13021 cttttccttt tctagggtaa agcagatcct gtttgcttaa ccccaatttt ccatgatgaa
    13081 tccaatagta aatggttgaa attcccacgt taaccccttt agccatcacc atcatttcag
    13141 gcgaaaattt ttggttatga tagtggagaa tcttttcctt tagttccttg gtcaagcttg
    13201 ttttcctgac cgagcgcttg cgattgtttt cataagactg ttgagcatag tcggcagaat
    13261 aaacctcttt gaagcgccct tttccaagac attgtcgaac tgtcccacgc ttgatttcag
    13321 tgtggatagt ttgaggagct tttccaagca gagaggcaat ttctctattt gatttccctt
    13381 cttttttcca tctttcgatt aagcgacggc tatcgattgt caaatgtttg gcttttgtag
    13441 tataattgtc ttgcatctct gtgcctttct tgtgtttgtg gttgaacaac aagttaacac
    13501 aaaggtgttt tcttatgcct acaagagctt tcattatttc cattttcttt tggatttcac
    13561 tctattctga aaaacttgtg tatatttact gaaactagca agtcttacct ataaatttaa
    13621 tgaaagcaac acaaaatccg agaggggaat ctcggattaa tgtcaattta aaaagataga
    13681 gagtttttag tttaaataaa ttgttttaaa atatcaacaa catcacttct tttcttaacc
    13741 tgataagcct tgattcctaa ttttagggct acgattatat tgtcctcaat atcgtctaga
    13801 aagacacaat ttctaggtta taactggtat ttatcgatag ttactcatat atatcagtcc
    13861 acctccatac tcaccttaca gattcctttg gtaataatat ctttgcctaa tgtagagata
    13921 gtcttgcaaa gaaaaaactt ccttgtagcc atgtttctga taaaagtcca gtgcctggaa
    13981 ctggtaagta ttgacaaagg caaaacaaca atttcgattc ttagcttcac tttctgcctg
    14041 ttgcaatagt tttgaaccga ttccttgccc tcgcagttgc tcttttacaa acaaatactc
    14101 gatttctagc caatttccaa aagtctctgc tatcaaaccc gcaaggagat tgcccttttc
    14161 atcttcgaca taaagattaa gtggctcact ttcagcctct tctctttttg aacggttata
    14221 agcacgaatc agattcccta tttcttgcga tttatatgat tccttatttt ccaatctaaa
    14281 gtataatgaa atgaaataaa acatgcgcaa atcgattaag gaatttaatc taatttctaa
    14341 caatgtctta gaaatcaaag tgtactattt taacttcaat gcactataca tctaatactc
    14401 aatgaaaatc aaagaacaaa ctaggaaact agccgcaggt tgctcaaaac actgttttga
    14461 ggttgtagat agaactgacg aagtcagtaa ccatatatac ggcaaggcga cgctgacgtg
    14521 gtttgaagag attttcgaag agtagcaaaa tggaaaaagg agtgagtgaa gcacatcgcc
    14581 tccccactcc tttttctgtt tttaggctgt tttttcaacc ttcaagattt ttacatcata
    14641 gctaccaaca ggcgtttcaa tggttgctgt gtcacctgtt ttcttgccaa tcaaggcctg
    14701 cccaattggg ctttcatttg aaaccttacc tgcaaaggca tccgcaccag ctgaacctac
    14761 gataatataa acttcttctt cgtcctcgcc aatttcttgg atggtgactg ttttaccaat
    14821 cgctacttcg tcctgggcaa ctgcgtcgct attgacgatt tcagcatagc ggatttttgt
    14881 ttctaagcta gagatttgtc cttcgacaaa ggcttgttca tccttagctg cttcgtactc
    14941 actgttttct gaaagatcac cgtatgaacg ggcaatctta atgcgttcta ccacttctgg
    15001 tcgacgaacc aatttcaatt cttctaattc tttttcaagt ttctcctttt cctcaagggt
    15061 cataggatat gttttttctg ccatttttct caactttctt ctgataatat tttctaaaga
    15121 aaattatgtg aagtatcaca taattttagt ttgtttagtt taatttgctg ttgacatgtt
    15181 cagcgacatt gcggtcgtgg tcttcttgat tgttagcata gtagaccttg ccttctgtga
    15241 catctgctac aaagtagagg ttatcgctct tagtttgatt gatgcttgac tcaatcgcat
    15301 ccagacttgg actatcgact ggaccaggca tgagacctac atttttataa acattataag
    15361 gtgaatcaat gttggtatca atcgcaacat cctcagctag actgatattt tgccccagtt
    15421 ttccttgggc atacaagatt gcaatattac tttgaagtgg catatcacga ttcaaacgat
    15481 tgtagaatac acctgcaatg agcttacgat cttctgtctt ggcaccttct ttttcgacca
    15541 aggaagcaat ggtcaacaac tcattgacag tcaagttttt agatttgata gtactatagt
    15601 aaggagatag gttcttatcc atagcagcta acatctcatc aatcaagctc tcaatagttg
    15661 tgctttcctt gatagagtat gtagctggga aaaggtatcc ttccaaacga taacgcgcac
    15721 cgctgtcttt tacaggcaaa ctttccagta aagtaggata tttcgctact gcttgactga
    15781 taaacgtctc atcttgaact ttagccaaga aagtctccgc tgtcaaagac tctttgaagt
    15841 caccttgcaa ttgacccaca gcttgagcaa tctgatccaa ggtataacct tctggaattg
    15901 tcaaagtcgc aagtacaggt tcttgcggtt catctgttcc acctttttgc aactctttga
    15961 gtaagtcttc tgtactcata ctcttttgca aattgtagta acctgctttc aagtcggtat
    16021 aatttttata cttggcataa aaactaaaaa tcagaccatg ctttaccaaa ccagcttttt
    16081 caagcgtcgt accgatttct tgaacgtttg aaccttctgg aattccaacc gtcacatatt
    16141 tcttagaatt agcatcgata ggtaacaaag aatctagcac gtactggtaa ccaaagtaac
    16201 cacctgctga gagcaaggca aggaatacaa ggaatgaaat cacaaaagct ttcacatgtg
    16261 acttcttctc tttcttaggc ttaacgggtt ttgctccttc tctacggcta cgacgtgatg
    16321 tatcgctgat gatatccact gtttctgtag caggcgtagg tgtttctgga cctgcttgtt
    16381 ctgctttttt ctctgccgtc gtataggaaa cagctaccct tgttggggtt tcattgtatt
    16441 ctctttcaag tttcttaggt ctaacaggac ctggacctgg tcttgatcca ctttcttccg
    16501 ctggagaaga aggtacgtct tgacttggat gacttggaac accaggagtt tctctttgaa
    16561 tctcatctgc tggagaagct ggtacacctt gacttgggtg agtaggcacg gtaggagctt
    16621 ttctcataat ctcctctacc gttgacaagg aatcagccat gagttcttca gctgaaggtt
    16681 catttgcagg agtgcgaact actgcctcat cttctttcag aacttcatca tagcctttta
    16741 ctttttctaa atctctcaga atctgctctt taaagcttaa tttctcttct tctcttgact
    16801 tttcactcaa aagtttttcc tccttgttga taatccataa tattatatct taaaagtcca
    16861 aaaaaagcaa tctatgatac ttttcctaac ttattttttc atttcccaga ccatatcata
    16921 ccatgtttcc cctgcaaagg ttgactggga ctggccttcg ttgacaaatc catgtttttc
    16981 ataataggcg atgagatagt catgacaggt tagattaatg ccatctctct catcttcaag
    17041 agcaacttct ttcaaggctg tcagcaattt ctgacccagt ccgagtccct gtgcctcctt
    17101 ggcaatagac agacaggtca cagagatata accaccaggc tcatgactat agtcttttat
    17161 ttcttctgta aaagactggt cttgcagatg gcggtgcagg ccaactggtc cttcgatata
    17221 acccatgatt cttccttctt tttcagcaac cagaaaagag gtctgaattt ctctcaaatg
    17281 tgcttcaaag acagaaggag gaatggcttc ttcgaccgaa aaattttcaa attcaagttc
    17341 aacaatccga tccaaatctt ctaatcttgc ttgtctgatt ttcattgttc ctccagataa
    17401 aagggattaa accaaatcat actatagccc tggctagttg catagagcaa agttgcttct
    17461 tcatcaacaa aaccgttcat ttcaaaatag gaaagcagct catcaggact ctccaaacga
    17521 atccctttgt aatccagctc aactgccacc tctttcaagg ctgcaagaag aagtgttccc
    17581 aagccctgtc tctgatggtc agactcgatg actaaagaat gtacttttag atattgcgga
    17641 ttgtctgact ggggacttga taaaatatag cctaaaagtt gattttcatc cctagctaga
    17701 agaaaggtat ccgcacactt acggatactt tcttctaaaa tatgggaaag ttgctgcttt
    17761 tcagctggaa aagacgaggt ctgaagtgcc cctatctcag gcaaatcaga cttgcttgcc
    17821 tgaatgatct taattggaat ttccatggga acatcctatt gaacattgct tgtcaagtta
    17881 gacaagagac gctcaaatga gtattcatag gtttggatgt ctcctgctcc cataaagacg
    17941 taaacagcat tgtcatggtc taggagcgga gaaacatttt caacagtaat cacttggtgt
    18001 tttttgttga ttttgttggc taggtcttct accttaacgt caccatgatc tacttcacga
    18061 gccgagccat aaatttgcgc tagataaaca gcatctgctt ggtttaaagc atgggcaaag
    18121 tcgtccaaca aggcaatggt tcttgtaaag gtatgcggtt gaaagactgc tacaatttcc
    18181 ttgcttgggt atctctgacg agccgcatcc aaggtcgcaa taatttctgt tggatgatgg
    18241 gcaaagtcat cgataatcac tgtatcattg acaattttct cagtgaaacg acgtttaaca
    18301 ccggaaaatg ttttcaagtg ctcacgcacc aagttcaaat caaatcctgc tgtgtaaaga
    18361 agaccaataa cggctgtcgc attcatgata ttgtgacgac caaaggttgg aatgtggaat
    18421 tgccccaagt tttgtccacg gaaatgaacg gtgaaggttg aaccagttgt tgaacgaaga
    18481 agatcactag ctacaaagtc attgccttca gcttcaaaac cataataata aattggtgca
    18541 tcagacgtaa tcttacgcaa ttcagcatct tcaccataga caaaaagacc cttggtaatt
    18601 tgtttggcat agtcgttaaa ggcattgaaa acatcctcga gacttgtgaa ataatctgga
    18661 tggtcaaagt caatgttggt gataatagag tattctgggt ggtaaggcat gaagtgacgc
    18721 tcatattcgt cagattcaaa gacaaaatat ttggcattag ccgaaccacg acctgtccca
    18781 tctccaatca agaagctggt atctgtaatg tgagacaaga catgagacaa catacctgtc
    18841 gttgaagttt ttccatgtgc tcctgctact cccatgctaa caaagtcacg cataaagcta
    18901 cctagaaact catggtaacg tttgtagctg ataccatttt ggtccgcata ggcaatttcg
    18961 acgttgttat ctggacgaaa ggcatttcca gcgataattt ccatatcacc gtctagattc
    19021 ttttcatcaa aaggaagaat ggtaattcct gcctgctcaa gaccgcgttg ggtaaagtag
    19081 tacttttcaa catctgatcc ctgaaccttg tgccccatct ggtgcaacat caaggccaag
    19141 gcactcatcc ctgatccctt aattccgata aaatgatatg tctttgacat gttttctccc
    19201 ctattctgtc actctggtca gattcaactc ttgggcaacc cgacgttctt gttctgtttg
    19261 tttacttttt ttattgtaga tttggctctt ctttagaaaa tcataattgt ttttctttgg
    19321 agcaggtgct gacacttctt cattcttggt agggatagaa tgaacttctt ccgccaagat
    19381 ataatgagac tgggtcaatt tttggctata tttgacaaat tcaccaggat tttccttttg
    19441 gaaaggagtt gtcggttgat tgccctgtct aactagactg ggctgagaat gacgtctcgc
    19501 aaggctgaaa ccctgagtta ggtagttagc agagcgtttc tttttcaagt ccgcacgcgc
    19561 ttcttcacgc gccacctccg catagctctt tccttctttt ttaaccccta aaggagcctt
    19621 tttaggtttc tcgacttgct tttcaatcgg ttttactggt ttttcttcag taataggagc
    19681 ccattctaaa taatttttat ctcgatactc acccttgata ttactgatca gatcagactc
    19741 atcatagaga ttcatgactg gcatttcagt caacatgacc tcgtcatctg acaccaatgg
    19801 aaatcgttct tgtttcattt tctatttcct ttcaacactt cattatagcg tattgtcttg
    19861 atttttcaag tgctgacttc agaaattccc aaaatttctc taatttctgc tagggtcaga
    19921 ctaccacgtg actctgtgcc gtccaatact tgtgacacca gatgtttctt ttgttcttgg
    19981 agttcctgaa ttttttcttc aatggttccc ttggtcacca agcgatagac ctcaaccgtt
    20041 tcttcctgac ccatccgatg ggcacggcca atggcttgcg cttccaccgc aggattccac
    20101 caaaggtcaa ccaagatcac tgtatctgca cctgtcaggt tcagaccgac cccaccagcc
    20161 ttgagggaaa tcagaaaggc atctctttct ccttgattaa aggccttggt catgtcttgt
    20221 ctttccttgg ctggggttga acccgtaatt ttaaaggaag tcaggcccaa gtctggcagt
    20281 tcttgttcaa ttttttccaa cattcccttg aactgagaga aaatcaagac acggtgtccg
    20341 ccgtctgcca cctgtaccag taggtctcgg agactatcta gtttaccgct ggctccctga
    20401 taatcttcca taaacagggc aggagtgtca catatttgac gcaagcgcat caaaccagat
    20461 aaaatttcca cacgacttcg ctgaaattcc tgttctgaca cttgagccag atggtctcgc
    20521 atctgttgta actgggcaag gtaaatagcc ttttgctggt cttccagttc atttttataa
    20581 accacctcaa tcaagtctgg caattcagtc agaacttctt ctttcttgcg tcgcatcacg
    20641 aaaggcttga taaactgagc cactcgctct gctggcaatt tcataaattc tttcttgctt
    20701 ggcaaaagtc cgggcatgac gatttggaaa atagaccaca actcacccag atggttttca
    20761 atcggagttc ctgacaaggc aaagaccgac ggcaccacaa attgtctcaa ggtctgggca
    20821 atcttggtct gggcattttt catgacctga gcctcatcta agaaaaggaa gtcaaaggcc
    20881 atcccctgat aaaactcact gtcctgacgg aaggtggcat agctagtcac atagatttga
    20941 tggctctcgg caagaatctc ctcacgactt gctttcaaac catgaacaac agccacatcc
    21001 aactgtgggg caaatttctg aaactcatcc gcccagttgt aaatcaaacc cgacggagcg
    21061 agaatcaaga ctcgactttc ttttgtcact tgactagtca aaaaagcaat ggtctgaagg
    21121 gttttcccaa gtcccatatc atcagccaaa atcccaccaa aaccataatg atggagcatc
    21181 tgcaaccagc caattccctt ttcctgataa tctcgcaagt cagccttgac ctgagttgct
    21241 tgcaaaggaa agtcctctgg atgcgtcaaa tcctgggcca gattctggaa ttcttgtgaa
    21301 aaagaaacac ggtctcgccc ttcaaagaga tgagctaaac tgtaggccaa ggatttccga
    21361 gcctgcaagg tcccatcttt taattcaaat tgccccagtt cctgtagatt ttggcgaatt
    21421 ttcttggttt cttcatcgaa aaagtaaact tgattagacg aatcaatata aaaatcctga
    21481 ttggcaacca aggcctgcat ggcttggtcg atttcctcct gggcaatatt ttgaaaatca
    21541 aactggattt ccaagagacc tcccttggag gcaatctgca cctgaggact cgctaggcta
    21601 taaagctctt ctagtttatc tgataggtca acatgcccga gtttttcaaa gactggaatg
    21661 atatcatgaa aaaaatgata gacagactcc gcttttaagg cctgacgcca agattgaaaa
    21721 tcggcttcaa agcccgcagc caaacagact tggaaaattc tttcttctaa gtccgcgtca
    21781 cttgaaaatg gtaattcttc tagctcttgt cggctagata cctgtctatt tccataatca
    21841 aactgaattt ctaaacgaat ccgattatct tcttccctgt caaagtaaaa agagggcgca
    21901 aaagttttga tttgtagacg ttctggagct gaaacggtgc ccatctggat aaaaagagtc
    21961 agacaggagg ccaatttgtc tcgatcactg ctatcaaatt gcaggtattt ctttccttgt
    22021 tgacccacag gtaacgcttt aatttccttg agaagacgca tctgctggtc tgttaaaaaa
    22081 taaacctgac ctttatggaa aagtactgct ccctgataaa agacattgac cctaggactc
    22141 tcactgattt ccatttcaaa atagtccgag tattctgtta ctgtaaaggc aaatagattg
    22201 gcatcagcat gcatatcctg aaaaagcagg gtttggtagc tatccacttg atggtcaaat
    22261 tgaaaatggg gcaaggccat cagtaaattc acaccctgct caaaaaaggt cagagggaaa
    22321 aagaggtgcc gaccttggtt ttggaaaaag aggtctggaa ccagcccttc ctccgttagt
    22381 ccgtgcaaga aagtcaaaag ttcttggctg gcatcatcaa aggcttccca agaaagagac
    22441 tcctcataaa tcttgccaat catatacgac tttctctgct cgacaatcct taaaaaaagt
    22501 ggaatatccc gaatgacata gtatttttgg ctattgattt ggccgattct cagagtccac
    22561 aagatatgat tggttcctgc ttccacctga cccacagctg ataactcata ggcgcattct
    22621 gattttggag ataaaatttg atccaaaaac ttgccaccca aggtcacctt ggtttcaaca
    22681 gcctcttttt cttcatgacc ttcttccaga ctccacaaga tttcctgacc acgctcatca
    22741 tttttcagaa aatgctctag cgctgccaaa tgcacacagt agcccctctt ttgaaaaaaa
    22801 tcacaggcac aaaaaaccaa atcatcctct aaactatagc gcagttcttc ttctgcaacg
    22861 cgagcgtaga gccgattgtt cttttccttg atgatatcaa ccttaccagt ttcataaagg
    22921 gcaacacctt cgatacgaat tttccccgga atcaatttag ccatattttc acctttacct
    22981 tatcttttta ttataccata ttttcgccta tgaaaatagc cttctaggaa gacttttctc
    23041 ctagaaggct ggatttttaa cgtttggcaa aagtagccac aatccgctga cagacttctt
    23101 gcaacagaga tttgggcata gctatattga tgcgggcatg gagacttcct tcctctccaa
    23161 aatccaaacc acggttgagg ataaccttgg cttcatttct caacaactct tgcaatgttt
    23221 catcagtcag gtcataagct gaaaagtcaa gccaaatcaa gtaggtacct tgcggtttca
    23281 tgaccttgat tttagtctct tttccaaata gatccatcac ataattgatg tggtcttcaa
    23341 agacttgctt gagttcctct agccaatctt taccgtatcg ataggcagct tctgtcgcca
    23401 aataacccaa gcctgaaatt tcatgctgat tattggccaa caggcgtttc tggaaagcca
    23461 gtctcaactt aggattttca atgactgcat aggaattttt tgttccagca atattaaatg
    23521 ttttagtggc actgctcaag acgatagcaa aatttttgaa ggcaggattg atggtattga
    23581 aagactggtg tttgtgacca aagagggtca aatcttggtg aatctcatcc gaaactaaca
    23641 aaacaccgtg tttttggcag agttggccaa tcttctccaa cacttctttt tcccaaacac
    23701 gtccaccagg attgtgaggg ttgcaaagaa catagagttt aacctcctct tccaccaaat
    23761 ccttttcaag ttggtcaaag tcaatctcaa acagactatc cttttccact aaggaattag
    23821 taatcaatct acgattattc aacttgacac tgcgatcaaa gggtgggtag acaggcgtgt
    23881 taattaaaac cgcctcgcct tcttttgtaa aggtttgaat agctgttgag atggctggta
    23941 ccacaccctc gataaagaca agagcctctt tgtcaaagtt gtaaccgtat tgtgtagctt
    24001 cccacttttg aacttcctta attaagtctt cactggcata ggtataacca taaaccagtt
    24061 ggtctgcgta agtttgcacg gcttggcgga tttcaggcaa gaccacaaag tccatatccg
    24121 ctatccaagc tggtagaact tcactatccg tttctgtttc tttccattta taggtatggt
    24181 gccctaaacg gttgggcagg cttgtaaaat catattttcc catctttgtc ttatccttct
    24241 atggcttggc gcaaatctgc aatcaaatct ctagcatcct caatcccaat agacaaacgc
    24301 aagaggtcat ctgtcaaacc ataagaatgg cgtacctctg ctggaatatc agcatgagtt
    24361 tgagtcgttg gataagtaat aagactttcc actccaccca aactttccgc aaaagagaag
    24421 accttgagac tgttcaaaat atgaggaatg cgtgtttcat cggctacttt aaaggaaatc
    24481 atgcctccac gaccagtgta gagaacttcc ttaactgctg gagaatcctt caaaaaggca
    24541 accacttctt gggcgttagc tgttgagcgc tccatacgaa gagacaaggt cttgagacca
    24601 cgaagcaact ggtagctgtc aaatggagac aagactgccc ctgttgtatt aagattgtaa
    24661 aaaagcttct cgtatagttc taaactattg gtcacaacca ctccagccaa gacatcattg
    24721 tggcctgcta gatacttggt tgctgaatgg agaacgatat ctgctccatc ttcaatcgga
    24781 cgttggtaga tagggctata gaaggtattg tccaccacca ctttggcacc cttagcatga
    24841 gccaattttg ctagtttttc gatatcaaat tccaacatca agggattggt tggggtttcg
    24901 atatagagaa catccacatc cttttctaac tcggcaatca actcttcttc tgtattggca
    24961 taggtaaaat ggaaatgacc ttcctgctcc acttggttaa accagcgaaa agaaccaccg
    25021 taaagatcac gcactgccaa gaccttactt cctactggaa agacgctaaa ggccagtaca
    25081 atagctgaca tccctgagct agtcgctagg gcatagtctg ctgactcaat agccgccaag
    25141 acttcctcag ccttactacg agttggattt ttagtgcgcg tatagtcaaa cccagtagat
    25201 cgaccaaact ctggatgctg ataggtcgtt gaaaaatgaa gtggtgtcac caaagcacct
    25261 gttgcctcat cagacttgat ccctgcttgt gctaaaattg tgttaatgtg taattccttg
    25321 ctcatacaat tcctccaaat ctatagtaac tattgtacca cttattttgt atccttcgtt
    25381 ttcttgtttt caagagctag ttatagtttc aaactatata aaaagggagt ttttcctgct
    25441 ccctttaata gactataaaa tggtgaatct caaaagacac cttcactcta tcatttgctc
    25501 ctgcacaaaa cgagcataac gctcatgatt ttccagtagt tccttatgag ttcctgagcc
    25561 agtgattttc ccctcctcta agaagaaaat acaatccaca tcttttaccg ttgacaaacg
    25621 atgcgctata atcacaaccg tcttctcctt taatgcagaa tagaggatac tgataatcgc
    25681 atactcagaa tccgcatcaa gattagcagt gaattcatca aatataagaa tttcagcatc
    25741 ttttaagtag gttctagcta tttgaagtct ttggctttgc ccccctgaca agaatcgtcc
    25801 gcgttcacca acttcagtat ctagtccctc tttcatggag cgaatctcat cacctagtga
    25861 tactaagtct agcactttca tcaattcatc atcagttact aagcgattca aaccgagaca
    25921 aagattgtca cgaatactgc cagataagac tgcattattt tgtgaaaccc aagcgatttt
    25981 acttctccat tcttttaagt taaaatcata tatacttgat tgctccatta gaatatctcc
    26041 tgcaagcggt ttataaaact gctctaacaa acgcacaatc gttgattttc ctgatccaga
    26101 tggtccaaca aaagcaattt tttgcccctt gaaaattgaa caagtaatat cctttaagat
    26161 aggtcgattt tcatcataac caaaatagac atggttaaaa ttcaaccctt gtcctgatac
    26221 cgattttcct cactcaaatt tttctttagg aactgcaagc aagttctcca gtgcaactga
    26281 agatcccttg ctcctagaat aaacagttac aaaattagct atattactaa taggattaag
    26341 taattgaaag aggtaaatca aaaacgaaac caaggttccc acagatatat atcctgcgct
    26401 gacccgataa cccccatagg ttagcatcac agctatagtc gcaaagataa ataagagagc
    26461 aaacggggtc tcaaaagaag taaccctatc tgatttcagt gaattgtttt gtaccctttc
    26521 aatacaatta tccaaaacat cctgtacact tttctctgct tggttagtct taattaattc
    26581 atgttcttga atattttcag tcaattgccc tgttaaaaat ccgatttcct cctgtaaacg
    26641 acgactatac ttttcactga tattggaaag gggcaagata ataaacatca tacaaggaag
    26701 agtgatgaat aaaagtagag aaagattcca atcaagacta aataagacta caatggaacc
    26761 aagtaccata actaaactca gaataatatt tgggaaagtc gtaattaaaa actcacgaat
    26821 gacactcgtg tcattgacaa tggcagaagt caactcccca ctttggctct tatcaaagaa
    26881 ggatttctct gcataaatca acccctctat cacttttttc ctgatttttg ctatcttttt
    26941 ttcacccgat tgactaaaca gatagtaacc aatagaagaa aacaaggctt gaccaataaa
    27001 aatcaaaaac gattgaaata ctttggaacc tatattttca atagaactcc catctattaa
    27061 accctttaag ataaggggaa gcaacaaagc aagtagacta gacagaacaa gtaagaaact
    27121 ccccataatc accttagtat ctactcttaa taattttaat ttcataaata ctccttataa
    27181 tatttcaacg gataaagtcg ggaataactc aatttgagga taaaa
//
LOCUS       DAVGJJ010000017        27097 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000017 DAVGJJ010000000
VERSION     DAVGJJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.017"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            147..281
                     /gene="rpmH"
                     /locus_tag="VVE35_000684"
     CDS             147..281
                     /gene="rpmH"
                     /locus_tag="VVE35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU3060921.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            565..1434
                     /locus_tag="VVE35_000685"
     CDS             565..1434
                     /locus_tag="VVE35_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU3060922.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGYGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGSSDANANEGGGSQAGGSAQSQASKQLATEKESAKNAIEKAAKD
                     KQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIVMVTV
                     PKRPVAPNAAPKTTSTPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLALAAATS
                     GFALLGRKTRRRK"
     gene            1739..2512
                     /locus_tag="VVE35_000686"
     CDS             1739..2512
                     /locus_tag="VVE35_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU3060923.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2512..3072
                     /gene="rnmV"
                     /locus_tag="VVE35_000687"
     CDS             2512..3072
                     /gene="rnmV"
                     /locus_tag="VVE35_000687"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU3060924.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3144..4043
                     /locus_tag="VVE35_000688"
     CDS             3144..4043
                     /locus_tag="VVE35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3060925.1"
                     /translation="MLIGQKIKEIRIEKGVSRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            4363..4659
                     /locus_tag="VVE35_000689"
     CDS             4363..4659
                     /locus_tag="VVE35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU3060926.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4736..6766
                     /locus_tag="VVE35_000690"
     CDS             4736..6766
                     /locus_tag="VVE35_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3060927.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLILSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6768..7409
                     /locus_tag="VVE35_000691"
     CDS             6768..7409
                     /locus_tag="VVE35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060928.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7402..7710
                     /locus_tag="VVE35_000692"
     CDS             7402..7710
                     /locus_tag="VVE35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060929.1"
                     /translation="MCEKIRIRRVSDYPSARDGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFREKR
                     "
     gene            7841..8713
                     /gene="rsmA"
                     /locus_tag="VVE35_000693"
     CDS             7841..8713
                     /gene="rsmA"
                     /locus_tag="VVE35_000693"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU3060930.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTTEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8715..9593
                     /gene="rsgA"
                     /locus_tag="VVE35_000694"
     CDS             8715..9593
                     /gene="rsgA"
                     /locus_tag="VVE35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU3060931.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9604..10260
                     /locus_tag="VVE35_000695"
     CDS             9604..10260
                     /locus_tag="VVE35_000695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU3060932.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            10223..10885
                     /locus_tag="VVE35_000696"
     CDS             10223..10885
                     /locus_tag="VVE35_000696"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU3060933.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            10887..12143
                     /locus_tag="VVE35_000697"
     CDS             10887..12143
                     /locus_tag="VVE35_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU3060934.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12133..13074
                     /locus_tag="VVE35_000698"
     CDS             12133..13074
                     /locus_tag="VVE35_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU3060935.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13341..14168
                     /gene="purR"
                     /locus_tag="VVE35_000699"
     CDS             13341..14168
                     /gene="purR"
                     /locus_tag="VVE35_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU3060936.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14235..15485
                     /locus_tag="VVE35_000700"
     CDS             14235..15485
                     /locus_tag="VVE35_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU3060937.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFI
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15611..16405
                     /gene="pflA"
                     /locus_tag="VVE35_000701"
     CDS             15611..16405
                     /gene="pflA"
                     /locus_tag="VVE35_000701"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU3060938.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(16480..17406)
                     /gene="yidC"
                     /locus_tag="VVE35_000702"
     CDS             complement(16480..17406)
                     /gene="yidC"
                     /locus_tag="VVE35_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU3060939.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPLLLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17484..17762)
                     /locus_tag="VVE35_000703"
     CDS             complement(17484..17762)
                     /locus_tag="VVE35_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU3060940.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17796..18536
                     /locus_tag="VVE35_000704"
     CDS             17796..18536
                     /locus_tag="VVE35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU3060941.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18592..19275
                     /locus_tag="VVE35_000705"
     CDS             18592..19275
                     /locus_tag="VVE35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU3060942.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19387..20379
                     /locus_tag="VVE35_000706"
     CDS             19387..20379
                     /locus_tag="VVE35_000706"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU3060943.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20444..20983
                     /gene="rsmD"
                     /locus_tag="VVE35_000707"
     CDS             20444..20983
                     /gene="rsmD"
                     /locus_tag="VVE35_000707"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU3060944.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            20973..21461
                     /gene="coaD"
                     /locus_tag="VVE35_000708"
     CDS             20973..21461
                     /gene="coaD"
                     /locus_tag="VVE35_000708"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU3060945.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDMIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            21445..22482
                     /locus_tag="VVE35_000709"
     CDS             21445..22482
                     /locus_tag="VVE35_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU3060946.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22540..22770
                     /locus_tag="VVE35_000710"
     CDS             22540..22770
                     /locus_tag="VVE35_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU3060947.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22794..24077
                     /gene="murA"
                     /locus_tag="VVE35_000711"
     CDS             22794..24077
                     /gene="murA"
                     /locus_tag="VVE35_000711"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU3060948.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24070..24258
                     /locus_tag="VVE35_000712"
     CDS             24070..24258
                     /locus_tag="VVE35_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU3060949.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24297..25121
                     /gene="endA"
                     /locus_tag="VVE35_000713"
     CDS             24297..25121
                     /gene="endA"
                     /locus_tag="VVE35_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU3060950.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25412..26743
                     /locus_tag="VVE35_000714"
     CDS             25412..26743
                     /locus_tag="VVE35_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU3060951.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(26942..27012)
                     /locus_tag="VVE35_000715"
     tRNA            complement(26942..27012)
                     /locus_tag="VVE35_000715"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26978..26980),aa:Cys,seq:gca)
BASE COUNT         7987 a         5054 c         6349 g         7707 t
ORIGIN      
        1 agggcttgca ttcgaaaaag ctggaaacat ttgcctagag ctattgacaa aagtgaaaga
       61 atcagataga atagtaaagt atgtttagta actgatcact ttatagccgg ctaaacgaat
      121 acaaaatcta tgaggaggta ttcatcgtga aacgtactta tcaaccaagt aaacttcgtc
      181 gtgcgcgcaa acacggattc cgtaaccgta tgtcaactaa aaatggtcgt cgcgtattgg
      241 cagctcgtcg tcgtaaagga cgcaaagttt tggctgcata atccaaacga attctatcaa
      301 aaatcagtag gaactcgagt ctactgattt ttatttttgt aaaaaagttc agtagatgca
      361 aatggattcg gaagcgatgt tacagtagat tgaaactaga atagtacacc tctgtttcta
      421 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttattt
      481 tactataaag ttgaagtagg tggagatggt acagcaacaa tcgtctttaa agatggttca
      541 gctattacaa ttccaggaaa tcaattggta gcacaagatc caaaagcaca agatagcact
      601 aaaccgactg ctgaaaaatc aactgttaaa gcacctgctc aaagagtaga tgtaaaagat
      661 ataactcatt taacagatga agaaaaagtt aaggttgcta ttttacaagc aaatggttca
      721 gcattagacg gagcgacaat caatgtagct ggatatggta cagcaacaat cacattccca
      781 gatggttcag tagtgacgat tctaggaaaa gatacagttc aacaatctgc gaaaggtgaa
      841 tctgtaactc aagaagctac accagagtat aagctagaaa ctacaccagg tggagataag
      901 ggaggcaata ctggaagctc agatgctaat gcgaatgaag gcggtggtag ccaggcgggt
      961 ggatcagctc aatcacaagc ttctaagcaa ttagctactg aaaaagaatc agctaaaaat
     1021 gccattgaaa aagcagccaa ggacaagcag gatgaaatca aaggcgcacc gctttctgat
     1081 aaagaaaaag cagaactttt agcaagagtg gaagcagaaa aacaagcagc tctcaaagag
     1141 attgaaaatg cgaaaactat ggaagatgtg aaggaagcag aaacgattgg agtgcaagcc
     1201 attgtcatgg ttacagttcc taagagacca gtggctccta atgctgctcc taagacaaca
     1261 agtacaccgc aagcaactgc aggaacaatg caagatgtta cctaccagtc acctgctggc
     1321 aaacaattac ctaacacagg ttcagcatca agtgcagcac ttgctagtct tgctctagcg
     1381 gcggcaacaa gtggttttgc tttgctagga agaaagacta gacgtagaaa atagaacagc
     1441 tagaaaattc tattctctac ttaaagttag attataagag ggattttgag aagtcatcaa
     1501 tcctagtgat gggtgagaaa agtgagaacc caagataatc acatacttta gctgaatagg
     1561 aatattctat caatgtagcc aatctcttct gtctctaact gtggaatagg agatgggtaa
     1621 tatcggatag aaaagatagc agaatagctc cctattgaag agaggagggg aaaccgaaaa
     1681 attaggtgcc cctcctcttt tttggtataa tagaagatag aaaacgaggt tagaagagat
     1741 gatttttgat acacatacac acttgaatgt agaagaattt gcaggtcgtg aggcagaaga
     1801 aattgccttg gctgatgaga tgggtgtgac acagatgaat attgttggtt ttgataaacc
     1861 gacgattgag catgccttgg agttggtaga tgagtatgag cagctctatg cgactattgg
     1921 ttggcatcct acagaagctg gtacttatac agaggaagtt gaggcttact tgttggataa
     1981 gttaaaacat tccaaggttg tggctttagg tgaaattggc ttagactacc attggatgac
     2041 agcgcccaaa gaggtgcagg agcaggtttt tcgccgtcag attcagctat ctaaggactt
     2101 ggatttgcct tttgttgtcc atacccgtga tgcgctggaa gatacctatg agattatcaa
     2161 gagtgagggc gttggtcctc gtggtggtat catgcattca ttttcaggga cgcttgagtg
     2221 ggcagagaag tttgtggatc ttggtatgac catttccttc tcaggagtgg tgactttcaa
     2281 gaaggcaact gacctccaag aagcagctaa agagttacct ttggacaaga tgttggtgga
     2341 aacagatgcg ccttacttag cacctgtacc caagcgtggt cgtgaaaata aaacagccta
     2401 tactcgctat gtggtcgact ttatcgctga cttgcgtggt atgacgacag aagagctggc
     2461 ggtagcaacg actgcaaatg cagaacgcat ttttggattg gacagcaagt aatgaaagag
     2521 agaatttccc aagttatcgt ggttgaaggt cgtgatgata cggtcaatct caagcgttat
     2581 ttcgatgtgg agacctatga gactcgaggt tctgccatca atgaccagga catagagcgg
     2641 attcagcgtc tacaccaacg tcatggagtc attgtcttta cagacccaga ttttaatggg
     2701 gagcggattc ggcgcatgat tatgacggcc attccaacag ttcagcatgc ctttctcaag
     2761 cgagatgaag ctgttcccaa gtccaagacc aaggggcgtt ctctgggaat tgagcatgcc
     2821 agctatgaag acctgaaaac ggctctagct caggtgacag aacaatttga acatgagagt
     2881 cagtttgaca ttagtcgtag cgacttgatt cgccttggtt ttctagcagg ggcagacagc
     2941 cgtaagcgta gagaatatct cggagagact ctccgaatcg gctattctaa cggcaagcaa
     3001 ctcctcaaac gcctagagtt gtttggggtt actttggcag aagtggaaga agctatgaaa
     3061 tcttatgagt aggaaagatg tagccgttac aattttttaa gtttcacagt atttttcgaa
     3121 gcaggtagaa gaggaggcgt ctgatgttaa ttggtcaaaa aattaaagag attcggatag
     3181 aaaaaggagt tagtcgtcca gatttttgtg gagatgagca agaactgaca gttcgtcaac
     3241 tgtcgcgaat tgaaagtgga gcttcgcaac cgagtttgcc caagttagcc tatattgctc
     3301 gccggctagg agttccagtt tatagcctta tgccggattt ttcagctctt cctcctgctt
     3361 atttagaatt gaaataccag attttacgtg aaccaatcta tggtaaagaa gaggagtacg
     3421 ataagaagga agcgtgtttg gaagagattt ataaaacata ctttgataat cttcctaaag
     3481 aagaacaatt agcatgtgaa gtattgcagg cgtgtttgga tacttctaga actagaaggc
     3541 ctgaatatgc agagttaata cttgaggaac atatgtctca gatcatagaa aaagaagttt
     3601 attcaacaaa tgatatgttg ttgattcgtt tgttttttta tcaaatgctc attagaaaag
     3661 atcttgccaa atttataaat caaatcgaaa agctaatgct ctttcttttg gaacagaaaa
     3721 aggtaactaa attagaaaac ttctttataa ttagagatac tcttatttca ggaatgtgtt
     3781 gtcttgaaaa ggtaggagta actgattgtt ttaatgatta tctatcgtgt ttacaagaaa
     3841 ttatggataa aactcaagat tatcaaaaga aacctcttgt atttatgttt ttgtggaagc
     3901 aagcattaag agaagaaaga gattttagtt tagctgaatc attttatcag tcttctaaaa
     3961 catttgcgca gctaattgga gatgaatttc tagtaaagaa attgacagag gaatggcaag
     4021 aggatgtcaa aaaatattta taaacatagt gaatcagtga caaaaatgtc cttgtcctcg
     4081 tatcaaaaca gttctaaagt tcgtctttag aactgttttt ttagatatag gctaaaaata
     4141 atatgaaata gttagatttt aaggacattg atgtctttga tagtgtgata aagatggagt
     4201 ataatgacta taaaagttca tttcagggtg ctattttgac aatttagaga aaaacaaata
     4261 attttagaat atcatttggt aatataatag tatcaacgtt gataatggaa agaggagcaa
     4321 gtaactcaac aatcttgtaa tcaaaaatag aggagtatca atatgaaaca aacagttaaa
     4381 aaattagctc ttgtagcgag cattgcagca acattaggtg gaagtgtagc agttgcctct
     4441 gcagccgttc aatacccaga aggtggtgtt tggacttatg gttcaggtaa cggtggtgct
     4501 tactcaaact actatcaccc ttcaaaatac catagttcaa cagttgttag cagaaaaact
     4561 ggttcatctg acaagggata tgctggtgct ggagggactt ctcgtgcatg gattcgtact
     4621 tcttggggag agaaagttgc attctactat aatgtttaga atggtatagc cctattgttt
     4681 agctttactt ggtcaccttc tcactaagtg accaagtaac tttttggagg aaatgatgaa
     4741 acgtttattt attttgattt caatggtatt ggtatcgctt tatatggtga taacttccgt
     4801 tgaccatcga gaagagattt tatttggtaa ctatccttct gttgatgtga caggaatgat
     4861 gacaaatcaa ccagtagcta gtcgcgaaga ggtgacagag gctttgagtc acttggcggt
     4921 agagcataat agtctcatcg cccgtcgaat cgttgagcca aatgaagctg gagaaacacg
     4981 ctttacctat gcaacttatg gtgagggaga gcttccagaa ggtctgacca tttcctccaa
     5041 ggagagtgca gaaacgagtg atttattagg gtcttacttg attgtgtcag gaagtttgga
     5101 tggagtgagc ttacagacca ccttgaaaga acttggctat caaggctttg tttcgaatgg
     5161 agaagatcca ttttcgatag tcttactatt gacggccacc cctatggtgc tactgagttt
     5221 agctattttt ctgctgacct ttatgagtct gaccctgatt tatcggataa aatcccttcg
     5281 tcaagcaggg attcgcttaa tagctggtga gagcttgttt ggagttgctc tcagaccagt
     5341 gttagaagat gtgagacagc ttatctgctc agtgctggta tccagtcttt tgggattggg
     5401 gattctctgg tatcaaggtg ccttgtttat ggcaacggtg caactggtca tcattgctct
     5461 tctactctat ggattgacct tggcagggat ttctacctta ctaagtgtcg tctatctact
     5521 tggtttacag gaaaatagtc tggtggatct attgaaaggg aaactccctc tcaaacgtat
     5581 gatgacattg atgatggtgg ggcaactctt agctgtattg gtggtcggat cgagtgcgac
     5641 agctctccta ccccactacc gtgaaatgca ggaaatggag agagctagca ataaatggag
     5701 ccagtcctca gaccgttacc gtctatcctt tggttggtct agtgcatttg ccgatgaaga
     5761 aggaacgcgt aaggataatc gtgagtggca gacatttact gaagaacggt tagccaatac
     5821 agactctttt tatattatga gcaatgttga caatttctca gatggagcag aagtggacct
     5881 agatggcaat cgtctcagtg actacacacc gtcagggaat gttatctatg tctcaccacg
     5941 ctatctgata gaagaaaaga ttaccgtttc ttcagagttt atggacaaga tgcaaaactt
     6001 gtctgaggga gagtttgggc tgatcttgcc tgagagcttg cgagagcagt ctgtctacta
     6061 ccaaggattg tttacagatt acctgcaaaa tttttcatct gaaagtgtag aagtgacgag
     6121 tcagaaacac tacctcccac aggtaaggct agcttttaca gaaacaggac aggaacgttt
     6181 cctctataat gatgggtaca agacaacacg ccagtaccta aaagatccga ttattgtagt
     6241 tctaacgccg caagcgactg gaacaagacc tgttgcaggg atgttgtggg gaactacggc
     6301 taatagtgcc ttgaaactag atcgatatgg agacagcatc acagctctaa aagagcaagg
     6361 tctgtatcac aaggtttctt acttggtaaa aagccagcta ttttttgcca aggtactaaa
     6421 tgacaaacgg gtggagtttt actctctcct tattgggacg attttgaccc tgtctacggc
     6481 tatcttgtta tttgattcca tgaatcttct ctattttgag cagttcagac gggagcttat
     6541 gattaaacgt cttgctggta tgacaatcta tgagcttcat ggcaagtatt tactggcgca
     6601 aggaggagtt ctcttgcttg gcttaatcct atctagtatt ttgacaagag atggtttgat
     6661 tagcgctcta gttgtagctt tgtttacgct taacgccctc ttgattttag taaggcagga
     6721 caaaaaagaa gaagctggta gcatggcagt attgaaagga aaataagatg attgatattc
     6781 aaggattgga aaagaaattt aatgaccgcg cgattttctc tggtttgaat ctcaagctgg
     6841 agaagggcaa ggtttatgcc ttaatcggaa agagtggaag cggaaagacg acgctgctga
     6901 atatcttggg aaagctagaa aagatagatg gtggaagggt tctctatcag gggaaagatt
     6961 taaaaaccat tcccactcgt gagtattttc gagaccagat gggctatctc tttcaaaatt
     7021 tcggcctctt agaaaaccaa tcaatcaaag aaaatttgga tttgggtttt gttggtcaga
     7081 aaatctcaaa agtagaacgt ttggaaaggc aagtgggggc tttagaaaaa gttaatctag
     7141 ggtatttgga tttagaacaa aaaatctata ctttatctgg gggagaggcc caacgagttg
     7201 cccttgctaa gactattttg aaaaatccac ccttgatttt ggcagatgaa ccaacagcag
     7261 ctcttgatcc tgaaaattca gaggaggtta tgaatctctt ggtggatttg aaagatgaaa
     7321 atcgaattat catcattgcg acccataatc ccctagtctg gaataaggct gatgaaatca
     7381 ttgatatgag gaaacttgct catgtgtgaa aaaatccgta ttcgcagggt atctgattat
     7441 cctagtgcca gagatggttt agaagatatc ctcatcatgg aaaatatgac caatcatctc
     7501 cttttggttc aaatccgagt gcatggctat ttgcttgatt ttgctagtat tgaagggcaa
     7561 aggcaaaagc attatcgttt gaaaaattta cctcaaacgg ttgaactgac agtggatgat
     7621 gtggaggagg atgtagattt gaccctacct gaaaatcgaa gttatcaaga agctgatttt
     7681 ttcgaacgta tgtttcgaga gaagcgctaa gtccctcttt aaagatttcc aagactatct
     7741 ttcttcatga ggaaagatag ttttttggta tgattttcat tcccaaaata caaggggaat
     7801 gtgttacaat agtagtaaca gataatagaa aagagaatag atgagaattg cagattatag
     7861 cgtgaccaag gcagtgctgg agcgtcacgg ttttaccttt aaaaagtcct tcgggcaaaa
     7921 tttcttgacg gataccaata tccttcaaaa aattgtggat acgactgaaa ttgatgacca
     7981 ggtcaatgtc atcgaaatcg ggccaggtat tggtgccttg acagaatttt tggctgagcg
     8041 tgcagcccaa gtcatggctt ttgagattga ccaccgtttg gtgccaattt tggcagatac
     8101 tctgcgtgat tttgataatg ttactgtagt caacgaggac attctcaagg ttgacttggc
     8161 gcagcatatc cagaatttta aaaatcctga cctgccaatc aaggtagtgg ctaatttgcc
     8221 ttactacatc acgacgccta ttctcatgca cttgattgag agtgggattc cttttagtga
     8281 gtttgtggtc atgatgcaga aagaagtagc ggaccgcatt tcagcccagc ctaacaccaa
     8341 ggcttacggt agcttgtcta tcgccgtgca gtattacatg actgccaagg ttgccttcat
     8401 cgtgcctcgt acggtctttg tgccagcacc aaacgtggac tctgctattt tgaaaatggt
     8461 gcgccgtcca gagccagctg tagcagtgga agacgagaac ttcttcttta aggtttccaa
     8521 ggctagtttc actcatcgcc gcaagacctt gtggaataac ttgacaggtt actttggtaa
     8581 gactgaagag gtcaaggaca agctgaccaa ggctttggac caggcaggct tgtcaccaag
     8641 tgtgcgtggg gaagctctca gcttggcaga atttgccggt ctagcagacg cacttaaagg
     8701 gcaaggactc taagatgcag ggacaaatca ttaaagcctt ggcaggtttc tactatgtgg
     8761 agagtgatgg ccaggtttat caaacacgcg cgcgtgggaa tttccgtaaa aaaggccata
     8821 ccccttatgt tggggactgg gtagatttct ctgccgagga aaattcagaa ggctatatcc
     8881 taaaaattca cgaacggaaa aacagtctgg ttcgtccgcc tattgtcaat atcgatcaag
     8941 ctgtagtaat catgtccgtc aaggaacctg attttaacag caatttgctg gatcgtttct
     9001 tggttctttt ggagcacaag ggcatccatc ccattgtcta tatttccaaa atggatttgt
     9061 tggaagatag gggagaactg gatttttacc ggcagaccta tggtgacatc ggctatgact
     9121 ttgtgaccag taaagaggaa ctcctgtctt tgttaacagg caaggttacg gtctttatgg
     9181 ggcagacagg tgttgggaag tcaactcttc tcaataaact cgtaccagac ctcaatcttg
     9241 aaacgggaga aatttcagac agtctaggtc gcggtcgcca taccactcga gctgttagtt
     9301 tttacaatct caacgggggt aaaatcgcag atacaccagg cttttcatct ctggactatg
     9361 aagtatcaag ggctgaagac ctcaatcagg ctttcccaga gattgctact gttagccgag
     9421 attgtaagtt ccgtacttgt acccataccc atgagccgtc ttgtgccgtc aaaccagctg
     9481 ttgaagaggg tgttattgca accttccgtt ttgataatta cctgcaattc cttagtgaaa
     9541 ttgaaaatcg tagagaaacc tataaaaaag tcagcaaaaa aattccaaaa taaggagaaa
     9601 cctatgtctc aatacaagat tgctccgtca attctggcag cagattatgc caactttgaa
     9661 cgtgaaatca aacgtctaga agcaactggg gcagaatatg cccatatcga tatcatggac
     9721 agtcattttg taccgcaaat cagttttggt gcaggtgtgg ttgaagcact tcgtcctcat
     9781 agtaagatgg ttttcgattg ccacttgatg gtgtcaaacc ctgagcatca tctggaagat
     9841 tttgcgcgtg caggtgcaga catcatcagt atccatgtag aagcaacgcc tcatattcat
     9901 ggcgccctcc aaaaaattcg ttcactcgga gttaagcctt cagtcgttat caatcctggc
     9961 acaccagttg aagccatcaa gcacgtcctt catctagttg accaagtttt agtcatgacg
    10021 gttaatccag gttttggtgg gcaagccttt ctgccagaaa ccatggataa ggttcgtgag
    10081 ttggttgctc ttcgtgagga aaaaggtttg aactttgaaa tcgaagtgga tggtgggatt
    10141 gatgaccaaa ctattgctca agccaaagaa gctggtgcga ctgtttttgt agcaggttcc
    10201 tatgtcttta agggagaagt caatgagcga gtacaaactc tcagaaaaca actggactag
    10261 ggttgccgtt tttgcaggcg gaaaccgcgg tcattatcgg acagattttg atgcttttgt
    10321 tggggtggat cgaggctcgc tctgggtctt ggaagaagac ttacctcttg ctctagcagt
    10381 cggagatttt gattctgtga cggaagaaga gcgacaggtg attcaaaaac gtgcccagca
    10441 ttttgtccaa gcccgaccag aaaaggatga tacagatctg gaattggctc tcttaaccat
    10501 ctttgaacaa aatcctcagg ctgaggtcac tattttcggt gccttgggtg gccgtattga
    10561 ccatatgttg gccaatgtct ttctacctag caatcctaag ttggcaccct atatgcgtca
    10621 aatagaaatt gaggatgggc aaaatttgat tgcctattgt tcagaaggga ccagtcagct
    10681 agaacctcgt tcagactacg actatctagc ctttatgcca gttcgggata gccagctgac
    10741 tattcttgga gctaagtatg agttgacaga ggagaatttt ttctttaaaa aagtgtacgc
    10801 ttctaacgaa tatatagata gggaagtgtc ggtgacttgc ccagatggtt atgtggtcgt
    10861 actgcatagc aaggacagga ggtaggatgg aaagtttact tattctatta ttaattgcca
    10921 acctagctgg tctctttctg atttggcaaa gacaggatag gcaggagaaa cacttaagta
    10981 agagcttgga ggatcaggca gatcatttgt cagaccagtt ggattaccgc tttgaccaag
    11041 ccagacaagc cagccagtta gaccaaaaag atttggaagt ggctgtcagc gaccgtttgc
    11101 aagaagtgcg gattgaattg caccaaggcc tgacccaagt ccgtcaagaa atgacagata
    11161 atctcctcca aactagagac aagacagacc aacgtctcca agccttgcag gaatcaaatg
    11221 agcaacgttt ggaacaaatg cgccagacgg tcgaggaaaa actagaaaag accttgcaga
    11281 cacgcttaca ggcttccttt gagacagttt ctaaacaact ggagtctgtc aatcgtggtc
    11341 ttggagaaat gcagacagtt gcccgtgatg tcggagctct taacaaggtt ctctctggaa
    11401 ccaagacgcg agggattctg ggagaattgc aactggggca aattattgaa gacatcatga
    11461 cacctgccca gtacgaacga gaatacgcaa cggttgaaaa ctctagtgaa cgagtggagt
    11521 atgccatcaa gttacccgga caaggcgacc aagaatacgt ctacctacca attgactcta
    11581 agtttccact ggcagattat taccgcttag aagaagccta tgaagtaggt gacaaggatg
    11641 aaatcgaacg ctgtcgtaag tcactcctag caagtgtcaa gcgttttgct agggatatta
    11701 ggaacaagta catagcacca cctcggacga ccaattttgg agttttgttt gttccgacag
    11761 aaggtcttta ttcagagatt gtgcgcaatc cggtcttctt tgatgatttg agacgggaag
    11821 aacagattat cgttgcagga ccaagtaccc tatcagccct tcttaactcc ctatcagttg
    11881 gtttcaagac ccttaatatc caaaagagtg ccgaccatat cagcaagact cttgccagtg
    11941 tcaagaccga gtttggcaag tttggtggta ttctggtcaa ggcacaaaaa catctccaac
    12001 atgcctctgg caatattgat gaattattaa accgtcgtac catagctatc gagcggacgc
    12061 tccgtcacat tgagttgtca gaaggtgagc ctgcgcttga tctactccat tttcaagaaa
    12121 atgaggaaga atatgaagat tagtcacatg aaaaaagatg agttatttga aggcttttac
    12181 ctaatcaaat cagctgacct gaggcaaact cgagctggga aaaactacct agcctttacc
    12241 ttccaagatg atagtggcga gattgatggg aagctctggg atgcccaacc tcataacatt
    12301 gaggccttta ccgcaggtaa ggttgtccac atgaaaggac gccgagaagt ttataacaat
    12361 acccctcaag tcaatcaaat tactctccgc ctgcctcaag ctggtgaacc caatgaccca
    12421 gctgatttca aggtcaagtc accagttgat gtcaaggaaa ttcgtgacta catgtcgcaa
    12481 atgattttca aaattgaaaa tcctgtctgg caacggattg tccgaaatct ctacaccaag
    12541 tatgataagg aattctactc ctatccagct gccaagacca accaccatgc ctttgaaacg
    12601 ggcttggcct atcatacggc gaccatggtg cgtttggcag acgctattag cgaagtttat
    12661 cctcagctca ataagagcct gctctatgcg gggattatgc tgcatgactt agctaaggtt
    12721 atcgagttga cggggccaga ccagacagag tacacagtgc gaggtaatct tcttggacat
    12781 atcgctctca ttgatagcga aattaccaag acagttatgg aactcggcat cgatgatacc
    12841 aaggaagaag tcgttttgct tcgtcatgtc atcctcagtc accacggctt gcttgaatat
    12901 ggaagtccag tccgtccacg tattatggag gcagagatta tccatatgat tgacaatctg
    12961 gatgcaagca tgatgatgat gtcaacagct ctggctttgg tggataaagg agagatgacc
    13021 aataaaatct tcgctatgga taatcgttcc ttctataaac cagatttaga ttgataattt
    13081 aagaaaaatg agcatttttt aggataagaa tgttcgtttt tttatgtgaa tatggtataa
    13141 taagtaaaag acaaaaatga atactcttcg aaaatctctt caaactaggg tagtatcgcc
    13201 ttgtcgtatg tatatatgca ggtatattac agggtttgtc agttctattg acaatctcaa
    13261 aacagtgttt tgaaccacca gcgaccagct ttctagtttg ctttttgatt ttttgaataa
    13321 aaatggaata ggaaatagaa atgaaattaa gaagaagtga tcggatggtt gtcatttcca
    13381 actatttgat taataatcct tataaactaa ctagtctcaa tacttttgct gaaaagtatg
    13441 agtctgctaa atcatccatc tcagaagata tcgtcattat caaacgcgcc tttgaggaaa
    13501 ttgaaatcgg tcatatccag acagtgactg gggctggcgg aggtgtcatc ttcacaccgt
    13561 ctatttcgag tcaggatgct aaggaaatgg ttgaagactt gcgtaccaag ttgtcagaaa
    13621 gtgaccgtat cttgccaggt ggttatatct atctgtctga tttgcttagc acaccagcca
    13681 tcttgaaaaa tattggtcgt attattgcca aaagctttat ggaccaaaaa attgacgcgg
    13741 ttatgaccgt agcaactaag ggtgtgccac ttgcaaatgc agttgccaat gtcctcaatg
    13801 tctcttttgt cattgtgcgc cgtgacctga aaattaccga aggttcaact gttagcgtca
    13861 actatgtttc aggttcaagt ggtgaccgta tcgagaaaat gttcctttca aaacgtagtc
    13921 ttaaggcagg cagccgtgtc ttgattgtgg atgacttctt gaaaggtggc ggaacggtca
    13981 acggtatgat tagtctcttg cgcgagttcg actcagaact ggcaggtgta gcggtctttg
    14041 cggacaatgc ccaagaagaa cgtgaaaagc agtttgacta caagtcactc ttgaaggtaa
    14101 ctaatattga tgtcaagaac caagccatcg atgttgaggt tggcaatatc tttgacgaag
    14161 ataaataaga gatagaacta aaggttggaa cgattgtccc agcctttctt tgcaaacaga
    14221 atagaaggaa gcttatgaaa acaccattta tcaatagaga agagttagaa gcgattgttg
    14281 ccgagttccc gactcccttt cacttgtatg atgagaaggg gattcgtgag aaggcaagag
    14341 ctgtcaacca agctttttcg tggaacaagg gctttaagga atattttgca gttaaggcta
    14401 ctccaactcc agctattttg aaaattctcc aagaagaagg ttgtggtgtg gactgctcta
    14461 gttatgtaga gcttttgatg agccataaac tggactttct gggttctgag attatgttct
    14521 cttccaacaa cacgccagac aaggaatacg cctatgcacg tgaattgggt gcgaccatta
    14581 acttggatgc ctttgaagat attgaacatc tggagagagt agcaggcatt ccagaaatca
    14641 tctcttgtcg ttataatcct ggaggcgttt ttgaactggg gacagacatt atggacaatc
    14701 ctggggaggc taagtttggc atgaccaagg accagctctt tgaagccttt gctatcttga
    14761 aggaaaaagg agccaagact tttgggcttc actccttcct agcgtccaat accgtgaccc
    14821 atctctatta tccagagttg gctcgtcagc tctttgaact ggctgttgaa atcaaggaaa
    14881 agttgggcat ttcgttagac tttatcaatc tttctggcgg tattggtgtt aattatcgtc
    14941 cagaccagga gccgaacgat atcgccttga ttggtgaggg agttcgtaag gtgtatgaag
    15001 aagttcttac gtcagcaggt cttggtcagg tcaagatttt caccgaattg ggtcgtttta
    15061 tactggcacc tcacggtgct ctagtcacaa gagtcactca taagaaggaa acctaccgta
    15121 cctatctagg tgtagatgcc tcagcagtca acctcatgcg tccagctatg tacggagctt
    15181 accatcatat tagcaacgtg acccatccag atggaccagc tgaagcggta gatgtggttg
    15241 gttcactctg tgaaaacaat gataaatttg cagtcaatcg cgaactgcct catacagaaa
    15301 tcggtgattt gctggtaatt catgatacag gtgcccacgg attttcaatg ggctaccagt
    15361 ataatgccaa attacgttct gcggaaatcc tctataccga agaaggtaaa gcccgtcaaa
    15421 tccgccgtgc agagcgccct gaggactatt ttgcaacctt atatggcttc gattttgaag
    15481 aataatctga taatagattg aaaatgaaat tgaaaaacag attgctttct aaaaaatagg
    15541 caaaaatctt gtttttcctt caagtcgtga tataataaaa ctataaaacg ttttcaagga
    15601 aggtaacgat atgtctgaag aaacaattga ttatggacaa gtgacaggaa tggtgcattc
    15661 gacagaaagc tttgggtcag tagatgggcc cggtattcgc tttattgtct ttttgcaggg
    15721 ctgtcacatg cgttgccagt attgccacaa cccagacact tgggctatgg agtccaataa
    15781 gtcacgtgaa cggacggtag atgatgtctt gacagaggcc ttgcgctacc gtggtttctg
    15841 gggaaataag ggtgggatta cagtcagtgg aggagaagct ctcttgcaga ttgatttcct
    15901 gattgccctc ttcaccaagg ctaaggaaca aggaatccac tgtaccttgg acacttgtgc
    15961 tcttcctttc cgtaataaac cacgttacct tgagaagttt gacaaactca tggctgtcac
    16021 tgacttggtt cttttggata tcaaggaaat caacgaagaa cagcacaaga ttgtcactag
    16081 ccaaaccaat aaaaatatct tggcttgtgc ccagtatcta tcagatattg gaaaacctgt
    16141 ctggattcgc cacgtgctag ttccaggatt gacagacaga gatgatgact tgattgaact
    16201 tggtaagttc gtcaagaccc tcaaaaatgt tgataagttt gaaattctac cttatcacac
    16261 catgggtgag ttcaagtggc gtgaacttgg aattccatat tccctcgaag gagtcaaacc
    16321 accaacagca gatcgcgtca agaacgctaa acaactcatg gataccgaaa gttatcaaga
    16381 ttatatgaaa cgtgtacatg gatagaaaag aagcctgatg gaaacatcgg gcttttgact
    16441 tgcaaaaaga cttagcaaat cagctaagcc tttttcttct tatctcgaac gttgttttcc
    16501 agcgttgcga tttttgtgtt ttttcttgct tgtaatagca gttggttgtt caggggtaac
    16561 gtcttttcgt ccacttggtg ttgagaaagc acttgctttt ggtgggttct ttgccagttc
    16621 ttcacggact tttttgcgaa gttttggacg aacgatatag ttgacgataa actgttggag
    16681 aatcatcatg aaaccaccga caacccagta aagtgtgaca ctagctggtg agaagaggga
    16741 gaagacgacg atcatgagtg ggctcatgta aatcattttc ttgatttgtt ctctttgcat
    16801 ttcatcttct actccgtgaa gtgaaaggag cgattgaaga tagtaaagga caccagcaca
    16861 ggcaaccaaa atcatacttg gagaacctag aggaatgcct aggtagcttg cttgagcaac
    16921 cccttcagta tgttgggcag caaagtagat agcagagaag aaaggcattt gaaggaggag
    16981 agggaaacat cctacaccgc caaacatgct gataccgtgc tctttttgag cagcaaagag
    17041 agcttgttgg gcttcgagtt tttcttcttg agtagtcgct tctttgagac gcgtttggtg
    17101 tggctcaagg acgtgcttga gggcgttcat cttttcagag tgaagcgttg ccttccatga
    17161 ttggtagata ccaagtggta agataatcaa gcgtacgata atggttacga taatgatagc
    17221 gacaccaaag cctagacctt tatcagtagc gaagtacttg atggcttcag ccataggcgc
    17281 tccgatggta ttccaaataa atcctgttgg ctgacctgtg gttttatcga cattgacaca
    17341 gccagtcaag acaagcaaca tagccactcc catagccgag aatgcaaaac gtttaataga
    17401 tttcactgtt tttattcctt ctttaaaaat tatacctttc tattctactg tttttttaca
    17461 aaatatacaa tagttctgga gacctaattt gcgattttaa agtcagagta gggaggaaag
    17521 ttgcttagtt tgacatctaa gtagcttact tttgaaaaag gtgtcggtcc ttttcggatt
    17581 tcttggataa attttgccat gatagcagat gagtctgctt gggctaagat ttccactgtg
    17641 ccatcgtcgt tattccatac tcgacctgtg atgccaccaa tttcaagtgc caagctgtaa
    17701 acaccccaac gaaagccgac tccctgtacc ctaccttggg caatcattct aaccttttgc
    17761 ataccaaacc cctttgattg tgttataata tttctatgac tattataacc tcaaaagcca
    17821 attctgtggt aaaaaatgcc aagaaattac accaaaaaaa ataccgcaag tctgcctatt
    17881 tgattgaagg ctggcacttg tttgaagagg ctgtccaagc tggagtgacg attgagaaaa
    17941 tctttgccct agaaaattat cgagatcagt tagctgcttt tccgcaaact gtctggattt
    18001 cagaggatat tttgctagat ttggcggatt ctcagactcc acagggaatt gtggcggtgg
    18061 ttcaaaaaga agaagtagga caagctgatt tgagtcaggg caagttcttg tttttggaag
    18121 atgtgcaaga tcctggtaat gtaggaacta tcattcgaac tgcggatgca gcaggtttta
    18181 caggagtgat tgtttcagat aagtcggcag acatctacag tctcaaaacc ctacgttcca
    18241 tgcaaggtag tcatttccat ctgcccattt accggatgac tagtcaagcg cttcttgacg
    18301 aaactaaaaa ggtagctatc ccagtgctag caacaaccct atctaaagat tctgttgatt
    18361 acagagaact gccttctata gaaaattttg tactagttat gggaaatgag ggtcaaggaa
    18421 ttagtcccct tatggctgaa agtgcagacc agttggtcca tattagcatg aaggggcagg
    18481 ccgagagttt gaatgttgca gttgcagccg gtattttaat cttccattta agctaatttt
    18541 aacttttttt gttataatca aggaaagatg ttcacagaaa aggagaaaag gatgaatcac
    18601 actattatac atgaccgtgc aggtctcaat caattttacg ctaaggttta tgcctttgtt
    18661 ggtctgggaa tcggactatc tgctttggta tcaggcctta tgttgacggt ctttcagtct
    18721 cagttggttt acttgttgat gcaggggcgt ctctggttga ccattgctac ttttgcggag
    18781 ttggctttgg ttttcgttgc cagtagtatg gcttcaagaa atagtccagc tgctcttcca
    18841 gtatttttac tttactccgt tttaaacggc tttaccctta gttttgtggt ggctttctat
    18901 actccgggta cagttttatc tgcctttgta tcgagcgccc ttctcttctt tgtcatggcg
    18961 gcagttggta tctttaccaa gaaagacttg agtgggattg gtcgagcaat gatggcagct
    19021 ctcattggtt tactgattgc tatggtagtc aatattttct tggctagtgg cttctttgat
    19081 tatatgatta gcgtagccat ggttttggtt ttttcagggt tgattgcttg ggataaccaa
    19141 aagattcgcc tcgcctatga acaatcacaa ggtcgtgtag cgacaggctg ggtggtgtca
    19201 atggctctca gcatctatct tgattttatc aatcttttcc taagtattct acgtatcttt
    19261 ggtcgcaatg actaaaaggt ctatgaaatc agtgttttga gtgacactga ttttttttaa
    19321 attttctctt ttcttttctt ggaaataggt gtataatacg tttattaaat ttttgaggag
    19381 ttgtctatga agaaaagttt tatccatcaa caagaagaaa tttcctttgt caaaaacact
    19441 tttacccagt atttgaaaga taagctagaa gttgtcgaag ttcaaggtcc tatcttgagt
    19501 aaggtcggtg acggaatgca ggacaacctg tctggtgtgg aaaatccagt atcggtcaag
    19561 gttctccaaa tccctgatgc tacttatgaa gtggtgcact cacttgctaa atggaaacgc
    19621 cacaccttgg ctcgttttgg ctttggtgaa ggagagggtc tctttgtcca catgaaagcc
    19681 cttcgtccag atgaggattc cttggatgca acccactctg tttatgttga ccagtgggac
    19741 tgggagaagg ttattccaaa tggtaagcgt aacatcgttt atctaaaaga aacagttgag
    19801 aagatttata aggctattcg cctgactgag ctagctgttg aagcccgcta tgacatcgag
    19861 tctatcttgc caaaacaaat cacctttatc catacagaag aattggtaga acgctaccca
    19921 gacttgacac ctaaagaacg tgaaaatgcg atttgtaaag aatttggagc cgtctttttg
    19981 attggtatcg gtggcgagtt gccagatggt aaaccgcacg atggacgtgc accagactat
    20041 gatgactgga caagcgagtc tgagaatggc tacaagggtc taaatggtga tattcttgtc
    20101 tggaatgagt ctttaggtgg agcctttgag ttgtcttcta tgggaattcg tgtagatgaa
    20161 gaaactctta gacgtcaggt ggaaattaca ggtgatgaag accgcttaga attggaatgg
    20221 cacaagtctt tgttgaatgg cctattccca ttgacaatcg gtggaggaat tggacaatct
    20281 cgtatggcca tgttcctact tcgcaagaga cacatcggag aagtgcaaac aagtgtttgg
    20341 cctcaagaag tccgcgatac ttacgaaaat attttgtaga gaatcgaacc gcaaggttcg
    20401 gttttctttc tctttttgtc tataatttgg tataataaac agtatgaaaa tcgtatcagg
    20461 aatctatggg ggacgtcccc tcaagacact agaaggcaag acaacaagac ctacttcgga
    20521 taaggttagg ggagccattt ttaacatgat tggtccctac tttgaagtgg gacgagtctt
    20581 ggacctttat gcaggtagtg gtggtttatc tatcgaagca gtatcgcgtg gcatgtccag
    20641 tgctgttttg gtggagcgag accgtaaggc tcagaccatc gtggctgaaa atatccagat
    20701 gaccaaggaa gttggaaaat ttcaactcct caaaatggat gcagaaaggg cattggaaca
    20761 ggtatctggg gaatttgacc tcgttttctt agaccctccc tatgccaagg aacaaatcgt
    20821 agcagatatt gaaaaaatgg ctgagagaga gcttttttct gaagatgtta tggttgtgtg
    20881 cgagacggat aaggccgttg aacttccaga agagattgcc tgtctgggta tctggaagga
    20941 aaagatttat ggaattagta aggtgacagt ctatgtcaga taagattggc ttattcacag
    21001 gctcatttga tccgatgaca aatgggcatc tggatatgat tgaacgggcg agcagactct
    21061 ttgataagct ttatgtgggt atttttttta atccccacaa acaaggattt ctccctcttg
    21121 aaaatcgtaa acgggggtta gaaaaggctg tgaaacattt gggaaatgtt aaagtcgtgt
    21181 cttctcatga taaattggtg gtcgatgtcg caaaaagact gggggctact tgcctagtgc
    21241 gaggcttgag aaatgcgtcg gatttgcaat atgaagccag ttttgattac tacaatcatc
    21301 agctgtcttc tgatatagag actatttatt tacatagtcg acctgaacat ctctatatca
    21361 gttcatcagg cgttagagag cttttgaagt ttggtcagga tattgcctgc tatgttcccg
    21421 agagtatttt ggaggaaata agaaatgaaa aaaaagatta gatggccctt atacgtcatt
    21481 gcggccttga ttgtgacctt cttggcattt gtagtgccct tgccttatta tatagaggtt
    21541 ccaggtggtt cggaagatat tcgccaagtc cttaaagtaa atgacacaga agataaggaa
    21601 gctggtgcct atcaattcgt tacggttggt gttcaacatg ccactttagc tcatatgatt
    21661 tatgcttggt tgacaccttt tacagatatt cgtagtgctc aggagactac aggtggttct
    21721 tccgatgttg aatttatgcg aatcaatcaa ttctacatgc aaacatcgca aaatatggcc
    21781 aagtatcaag gactaaaaac agctggtaag gatatcgaac tcaagtactt tggagtttat
    21841 gttttgaatg tgacggataa ttcaaccttt aaagggattc tcaatatctc tgatacagtc
    21901 acagcagtca atgatcagac ctttgatagt tccaaagact tgattgatta cgtcagttct
    21961 caaaaattag gggattccgt caaggtcacc tatgaagagg atgggcaaac caagtctgca
    22021 gaagggaaga ttatcacctt ggaaaatgga aaaaatggga ttggaatcgg cttgattgat
    22081 cgtacagagg taatcagcaa tgtcccaatt agcttttcaa cagctggtat tggcggccca
    22141 agtgctggtc tcatgtttag tctagctatc tatactcaaa tagctcaccc agatcttcgt
    22201 aatggtcgta ttgttgccgg tacaggtacc attgaccgcg atggtaatgt gggagacatt
    22261 ggaggtattg ataagaaggt tgtagcttcg gctagggcag gtgctgctat tttctttgct
    22321 cctgataacc ctgttagcga agaagaacaa aaggcgcatc cggacgcgaa aaacaactac
    22381 caaacagccc tagaagcggc taaaacaatc aagacggata tgaaaatcgt gcccgttaaa
    22441 accctacaag atgcgattga ttacttgaaa aacaatccct aatttttgag tgaagtttcc
    22501 aaactagcgc acaaacagag aagatggtat aatagtcaaa tggttcaatt attattcact
    22561 ctaagtagtc atatgttctt tatttatgtg agtttttacc ttttaaagga tcttgttaga
    22621 tgggagaagg ttttaaaagt gacagatgat aatacaagaa aagttcgttt attagtagcc
    22681 ttttttagca ttgtcatagg ctacatcctg agttctttct ttattagcct gtatcatttg
    22741 tggcaagaag cgcttagagg attattatga aatcaagagt aaaagaaacg agtatggata
    22801 aaattgtggt tcaaggtggc gataatcgtc tggtaggaag cgtgacgatc gagggagcaa
    22861 aaaatgcagt cttacccttg ttggcagcga ctattctagc aagtgaagga aagaccgtct
    22921 tgcagaatgt tccgattttg tcggatgtct ttattatgaa tcaggtagtt ggtggtttga
    22981 atgccaaggt tgactttgat gaggaagctc atcttgtcaa ggtggatgct actggcgaca
    23041 tcactgagga agccccttac aagtatgtca gcaagatgcg cgcctccatc gttgtattag
    23101 ggccaatcct tgcccgtgtg ggtcatgcca aggtatccat gccaggtggt tgtacgattg
    23161 gtagccgtcc tattgatctt catttgaaag gtctggaagc tatgggggtt aagattagtc
    23221 agacagctgg ttacatcgaa gccaaggcag aacgcttgca tggcgctcat atctatatgg
    23281 actttccaag tgttggtgca acgcagaact tgatgatggc agcgactctg gctgatgggg
    23341 tgacagtgat tgagaatgct gcgcgtgagc ctgagattgt tgacttagcc attctcctta
    23401 atgaaatggg agccaaggtc aaaggtgctg gtacagagac tataaccatt actggtgttg
    23461 agaaacttca tggtacgact cacaatgtag tccaagaccg tatcgaagca ggaaccttta
    23521 tggtagctgc tgccatgact ggtggtgatg tcttgattcg agacgctgtc tgggagcaca
    23581 accgtccctt gattgccaag ttacttgaaa tgggtgttga agtaattgaa gaagacgaag
    23641 gaattcgtgt tcgttctcaa ctagaaaatc taaaagctgt tcatgtgaaa accttgcccc
    23701 acccaggatt tccaacagat atgcaggctc aatttacagc cttgatgaca gttgcaaaag
    23761 gcgaatcaac catggtggag acagttttcg aaaatcgttt ccaacaccta gaagagatgc
    23821 gccgcatggg cttgcattct gagattatcc gtgatacagc tcgtattgtt ggtggacagc
    23881 ctttgcaggg agcagaagtt ctttcaactg accttcgtgc cagtgcagcc ttgattttga
    23941 caggtttggt agcacaggga gaaactgtgg tcggtaaatt ggttcacttg gatagaggtt
    24001 actacggttt ccatgagaag ttggcgcagc taggtgctaa gattcagcgg attgaggcaa
    24061 gtgatgaaga tgaataagaa atcaagctac gtagtcaagc gtttactttt agtcatcata
    24121 gtactgattt taggtactct ggctctagga atcggtttaa tggtaggtta tggaatcttg
    24181 ggcaagggtc aagatccatg ggctatcctg tctccagcaa aatggcagga attgattcat
    24241 aaatttacag gaaattaggc tggagaacca gcctttttct aaagataagg agaaatatga
    24301 acaaaaaaac aagacagaca ctaatcggac tgctagtgtt attgcttttg tctacaggga
    24361 gctattatat caagcagatg cagtcgacag ctaatagtcc taaaaccaag cttagtcaga
    24421 aaaaacaagc gcctgaagct cctagtcaag cattggcaga gagtgtctta acagacgcag
    24481 tcaagagtca aataaagggg agtctggagt ggaatggctc aggtgctttt atcgtcaatg
    24541 gtaataaaac aaatctagat gccaaggttt caagtaagcc ctacgctgac aataaaacaa
    24601 agacagtggg caaggaaact gttccaaccg tagctaatgc cctcttgtct aaggccactc
    24661 gtcagtacaa gaatcgtaaa gaaactggga atggttcaac ttcttggact cctccaggtt
    24721 ggcatcaggt caagaatcta aagggctctt atacccatgc agtcgataga ggtcatttgt
    24781 taggctatgc cttaatcggt ggtttggatg gttttgatgc ctcaacaagc aatcctaaaa
    24841 acattgctgt tcagacagcc tgggcaaatc aggcacaagc cgagtattcg actggtcaaa
    24901 actactatga aagcaaggtg cgtaaagcct tggaccaaaa caagcgtgtc cgttaccgtg
    24961 taacccttta ctacgcttca aacgaggatt tagttccctc agcttcacag attgaagcca
    25021 agtcttcgga tggagaattg gaattcaatg ttctagttcc caatgttcaa aagggacttc
    25081 aactggatta ccgaactgga gaagtaactg taactcagta aaagataagc ctaaactcct
    25141 atgtcactta tggatgtagg agttcttttt actagtttaa gcagggctag aacaggtact
    25201 aagaaaaaat agtaacttct aaaactaact tccagtttcc cacaacctag cttttagtat
    25261 gagaaaaacg cgtgaaatca gtggaaatcc gtcttagact aacttccagt tttgggagag
    25321 agatggaagt tacgctaaga aaaaaataat atgtattgga ttaaattttg tgatataata
    25381 aaggataaga tactatttta ggagaagaac tatggaagac ccaagtagtc agaatttgtt
    25441 gctacaattt gttttattgt ttattttgac ggtattaaat gcctttttct cagccacaga
    25501 aatggccatg gtttctctta atcgtgcccg agttgaacaa aaggcggaag aaggagacag
    25561 acgctacatt cgcctgctga aggtactaga aaatcctaac cactttttat caaccattca
    25621 ggtaggaatc accctgatta caatcttatc aggggcgagt ttaactgata ctctaggacg
    25681 tgtgattgcc tcttggcttg ggaatggcga aacagcacaa gccgtggcaa cttttctatc
    25741 cttggcattt ttgacctata tttccatcgt ttttggggaa ttatatccta agagaatcgc
    25801 tcttaatcta aaggatgcct tggcaattcg tacagcgccg gttatcattg ggatcggaaa
    25861 actagtcagt ccttttgttt ggcttttagc tgcttcgacc aatttcttga gccatttgac
    25921 tcctatgtca ttcgatgatg cggatgaaaa gatgacccgt gatgaaattg cctatatgct
    25981 gacaaatagt gaagaaacat tggatgctga tgagattgag atgctacaag gtgtcttttc
    26041 gctcgatgaa ctgatggcac gagaggttat ggttcctcga acggatgcct ttatggtgga
    26101 tattcaggat gatagtcaag ccattatcca aagtatttta aaacaaaatt attctcgtat
    26161 cccggtttat gatggggata aggacaatgt aattggaatc attcacacca agagtctcct
    26221 taaggcaggc tttgtggacg gttttgacaa tattgtttgg aagagaattt tacaagatcc
    26281 actttttgta cctgaaacta tttttgtgga tgacttgcta aaagaactgc gaaataccca
    26341 aagacaaatg gcaatcttgc tggatgaata cggtggtatg gctggtttag tgactttgga
    26401 agacctctta gaggaaattg ttggtgaaat tgatgatgaa acggacaaag cagcaataga
    26461 tgttcatcag attggcgagg atacctacat tgttcaagga actatgaccc tcaatgattt
    26521 caataattac tttgatgttg aactggaaag tgatgacgtt gataccatcg ctggttatta
    26581 tttgacagga gtgggaacca ttccaacgac tgagaaactc agctatgaat tggtcagcca
    26641 aaacaaacag tttatcttaa ccaatgataa agtgaagaat ggacgtgtta ccaaggtaaa
    26701 agtccaaatc acagaagtcg aaatagaaga agaaacagaa taaactagag gcttttgtca
    26761 ccaggcgtga cgaagctttt ttcaattgat ttcaaaaggg aattatgcca tggaaagaac
    26821 tctgccacaa actcgccaca aaacttttct aaatctattc gtatttatcc gaaaaaaaaa
    26881 taaaaaagcc ctattttacg ggctttttgt ctttataaat tccgattaat tcggtactga
    26941 aaggcggtag acggatttga accgacgatc aagcttttgc agagccgtgc cttaccactt
    27001 ggctataccg ccttaacgtt tattattata ccttgaaaat gattttcagt caatagtttt
    27061 tttagttttt tgtaccatag cgtgattcga atgtgat
//
LOCUS       DAVGJJ010000018        27094 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000018 DAVGJJ010000000
VERSION     DAVGJJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.018"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(221..1828)
                     /locus_tag="VVE35_000716"
     CDS             complement(221..1828)
                     /locus_tag="VVE35_000716"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU3060952.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2218..2805)
                     /locus_tag="VVE35_000717"
     CDS             complement(2218..2805)
                     /locus_tag="VVE35_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU3060953.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2933..3382)
                     /locus_tag="VVE35_000718"
     CDS             complement(2933..3382)
                     /locus_tag="VVE35_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060954.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRIKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3387..3776)
                     /locus_tag="VVE35_000719"
     CDS             complement(3387..3776)
                     /locus_tag="VVE35_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU3060955.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3804..4430)
                     /locus_tag="VVE35_000720"
     CDS             complement(3804..4430)
                     /locus_tag="VVE35_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU3060956.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4444..5007)
                     /locus_tag="VVE35_000721"
     CDS             complement(4444..5007)
                     /locus_tag="VVE35_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3060957.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5172..5406)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5460..5963)
                     /locus_tag="VVE35_000722"
     CDS             complement(5460..5963)
                     /locus_tag="VVE35_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU3060958.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6156..6986)
                     /locus_tag="VVE35_000723"
     CDS             complement(6156..6986)
                     /locus_tag="VVE35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3060959.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLVIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLILQNQGRFYNPWK
                     "
     gene            complement(6971..8653)
                     /locus_tag="VVE35_000724"
     CDS             complement(6971..8653)
                     /locus_tag="VVE35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3060960.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8755..8967)
                     /locus_tag="VVE35_000725"
     CDS             complement(8755..8967)
                     /locus_tag="VVE35_000725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060961.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(8974..9522)
                     /locus_tag="VVE35_000726"
     CDS             complement(8974..9522)
                     /locus_tag="VVE35_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU3060962.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9537..10385)
                     /locus_tag="VVE35_000727"
     CDS             complement(9537..10385)
                     /locus_tag="VVE35_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU3060963.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKMEG"
     gene            10573..11922
                     /gene="trkA"
                     /locus_tag="VVE35_000728"
     CDS             10573..11922
                     /gene="trkA"
                     /locus_tag="VVE35_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU3060964.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11926..13365
                     /locus_tag="VVE35_000729"
     CDS             11926..13365
                     /locus_tag="VVE35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU3060965.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISILSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13490..13747)
                     /locus_tag="VVE35_000730"
     CDS             complement(13490..13747)
                     /locus_tag="VVE35_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEU3060966.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            14160..14330
                     /locus_tag="VVE35_000731"
     CDS             14160..14330
                     /locus_tag="VVE35_000731"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3060967.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            14927..15211
                     /locus_tag="VVE35_000732"
     CDS             14927..15211
                     /locus_tag="VVE35_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060968.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSEELTHFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            15204..15442
                     /locus_tag="VVE35_000733"
                     /pseudo
     CDS             15204..15442
                     /locus_tag="VVE35_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15538..15756
                     /locus_tag="VVE35_000734"
     CDS             15538..15756
                     /locus_tag="VVE35_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060969.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(16123..18447)
                     /gene="pflB"
                     /locus_tag="VVE35_000735"
     CDS             complement(16123..18447)
                     /gene="pflB"
                     /locus_tag="VVE35_000735"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU3060970.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            18795..19856
                     /gene="dinB"
                     /locus_tag="VVE35_000736"
     CDS             18795..19856
                     /gene="dinB"
                     /locus_tag="VVE35_000736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU3060971.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNHIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(20003..20848)
                     /locus_tag="VVE35_000737"
     CDS             complement(20003..20848)
                     /locus_tag="VVE35_000737"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU3060972.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            21104..22131
                     /locus_tag="VVE35_000738"
                     /pseudo
     CDS             21104..22131
                     /locus_tag="VVE35_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(22432..24321)
                     /locus_tag="VVE35_000739"
     CDS             complement(22432..24321)
                     /locus_tag="VVE35_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU3060973.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKDQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            24459..26024
                     /locus_tag="VVE35_000740"
     CDS             24459..26024
                     /locus_tag="VVE35_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU3060974.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            26024..26764
                     /locus_tag="VVE35_000741"
     CDS             26024..26764
                     /locus_tag="VVE35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3060975.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(26873..27088)
                     /locus_tag="VVE35_000742"
     CDS             complement(26873..27088)
                     /locus_tag="VVE35_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060976.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         8280 a         5879 c         5036 g         7899 t
ORIGIN      
        1 taaaactttg ctagaaattg atttggcttt ccgaattaat ttgttcaaat tttatttcat
       61 ttcaatatac tgataaatcc attcactagc acagggcaag agggagtcgc tattgatttc
      121 aacggtagca ggtacataca gaggacccct acctcagata tactgcaata tcatcgtatg
      181 caaaaaacct ctgagatttt tctcaaaggt tctgattttg ctaattgctg ttctcaaccg
      241 ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt tggacggctt gacagttcag
      301 ggtgatactg acaagctaca aagaatttat tttcaggaat ttccacgatt tctaccaaac
      361 gattgtctgg agaaactcct gaaaagacaa aacctgctgc ctcaaactgc tcacggaagg
      421 cattattaaa ctcataacgg tgacggtgac ggcgttgcac cacttcttga ttgtgataag
      481 cagcagcagc cttagagcca cgtttcaact tagacggata aagtcccaaa cgaagggttc
      541 cacccatatc ctcaatatca atctgatcac gcatgatatc aatgataggg tattttgttt
      601 ctggtgcaag ctctgcagaa ttggcacctt caagacctaa aacgtgacga gcaaactcga
      661 tacatgtcaa ctgcattccc aagcagactc ccaacattgg aacatcattt tcacgcgcat
      721 agcggatggc ttggattttc ccttctgtac cacgttgacc aaaaccacct ggtacgatga
      781 tcccgtccgc atcagacaag agttctgcta cattctctgc tgtcacatca ttggcattga
      841 cccaattgat tttaacttct acatcattga catagccaga gtgtttcaag gcttcgacca
      901 ctgagatata ggcatcttgc aactccacat acttaccaac aagggaaatc ttaacttgtt
      961 tcttgaggtt catgaccttg tccaccatgg ctgaccattc tgtcatatcc gctgctggtg
     1021 cgtctaattt caaatgatca caaacaattt ggtccatccc ttgtgcctgc aagttcagtg
     1081 gaatttggta aaggtgttca acatccaacg attcgataac ggcttctggt gccacatcac
     1141 agaactgggc cagtttattt ttaattcctt gaccagctgg ctcttctgta cgaataacca
     1201 acatatttgg ttggattccc aagccacgca attctttgac agagtgttgg gttggtttgg
     1261 ttttcatttc accagcagcc ttgaggtaag gaagcaaggt tgtatggata tacatgacat
     1321 tatccgcacc cacatctgcc ttcatctgac gaagagcctc tagaaatggc aaggactcga
     1381 tatctcctac tgttccacca acctctgtga taatgacatc agagtcggtc gttagagcgg
     1441 cacgcttgat tttttctttc aaagcatctg tgatatgagg aatgacttga acagttgccc
     1501 caaggtattc tccacggcgt tctttacgaa gaacttcact gtaaattttc ccagttgtca
     1561 cgttggaata tttgttgaga ttgatatcga tgaaacgttc atagtgaccc aagtccaaat
     1621 ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga ctcatggttc
     1681 ccggatcaat attgatataa gggtcaaact tttgaatggt tactttgaga ccacgatttt
     1741 tcaagagacg gcctagactc gctgccacaa tccctttccc aatagacgat accacaccac
     1801 cagttacaaa aatatatttc gtagacatag attcctcttt ctaaaatgct caaggtcttg
     1861 ttaactacga ggggatatag aaaacaagac cctactaata agaataatct gggacgactg
     1921 caccagattc acagctaata ctcttcgaaa atctcttcaa accgcgtcaa cgtcgccttg
     1981 ccgtagatat gtgttcctga ctttgtcagt cttatctaca acctcaaaat agtgttttga
     2041 gcagcctacg gctagtttcc tagcttgctc tttgattttc attgagtata aaatacaaga
     2101 agataacttc ttgaaaaaac aaaaatagct ccctaagaac tagggagccc cgacctctaa
     2161 aagaggtgcc caaacaatat gatacctaaa aataggataa ttgtcaatag ctaactctta
     2221 ttcctcgctg gtttcagcat cgtcatctga aaactcgtcg tcgtcttcgt tgagatccac
     2281 atcttctccc aagtcatctg gagcgatttc gttgatttct gcatcataag cttccacttc
     2341 atttttttca tcatctggat tttcatcatc gtatgaaaga gctggatctg cttcgtatgc
     2401 atcttcgtct tctggatcat ctgcattgta gtcaatggca tctgaatcac catccataaa
     2461 ggcattgaca cgttttttct tagcttttgg tgctacttca tcgtcgtcat tttcttcaag
     2521 agcgatgatt tcttcgtcga tttcgtccac accataccat gaacgaagac cccatttgtt
     2581 gtccccaagt gagatgaagc taccgtcaaa gttcaactct gtgtagaaca aaggcaaagc
     2641 ttcgcggata tcgctgtttg atgttccaag gtagttttga atttcgttta caagatcgct
     2701 aaaatgcatc tcatgatcgc gaccacgaag ttccaagata gcacgcgcta cctcaatcat
     2761 agatagttca cttttttctt gcccagcaaa tacttctaat tccaaagcgt ttctcctcat
     2821 ttatactact atcgccagag cgaacagact ctgacctcat tttatcattt actctttatt
     2881 ttacgataat tttgcggaat agtcaaaggt taagggggag aaagtggcag gattagacta
     2941 attccaatat aaaactcctt cctttttctg ttgctccatt ttccacaaat ccaagcgact
     3001 tgaaacacct cctagaagca tgattgtagg tgtagatttc cttgattctc aattctttcc
     3061 atccttttac tcgagccaat tcaatcaaag cacttagaat cttttttcca agtcctcgat
     3121 gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt gtaatatccc
     3181 caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca ccatgccgat
     3241 ccaaatagga atacatggct tccaaggtcg cttgactgta aggaagcttc accccatcta
     3301 cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa taattgacct
     3361 tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc tgcttgatta
     3421 aaaccatcac accagttata ccattttgct tcatacccat cttgagctag gatacgatct
     3481 tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata gagatgatat
     3541 agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc tgccaataaa
     3601 gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa ggcaagaata
     3661 ctgattggat cgacttggat tgtttctgcc gactggtaaa gcatctgaat atcttgcaac
     3721 ttcttttctg ataaatatag ttgagtcgga tgaagatctg ctatattgac tttcatttct
     3781 ttctcctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca ttggaagcgg
     3841 agcttgtcat agaagggaaa ctcgataaac aggactccca agcccacaca gagactggca
     3901 aggacgtctg atgggtaatg aactcccaga tagactcttg ataccagcac actgactagg
     3961 tagaggccaa ggacgatttg tacgattttt ctccagactg gatctttaat ccgctgacta
     4021 agaataacaa tcaaagtacc taccatcaag gttacagcca gagaatgccc acttgggaag
     4081 gaaaatccct tctcctccac cagatgtaag atatctggcc gtgggcgctg gtagatattt
     4141 ttaaaggtca cgattaaaag acctgccaaa gccagatttc ccagcatgaa gaaactttct
     4201 atcttccatc gcttacgata aaagacaaaa gctgtaatga caacccaagt gataatcact
     4261 gggatatcaa tcagacgtgt gagggctcga aaaagaatag tcaaataatc tggtaagtct
     4321 cctcgaatgg cagtctgaat cgattggtca aaattgacca gcgtttcagg gtaaaatttg
     4381 accatgtagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat taaaaatgtt
     4441 tgtttatctc tcataatgtt ttaaggttgg tttcaagaga acatacaaca accagaatga
     4501 aacggaaaag ataacacctt caatcaagtt aaaaggtaat accatggtca ttaggtagtt
     4561 ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca aaggaacagc
     4621 ataaacatag ttgagaacca acatggccaa ggttaaacca acagttccag ctagagagcc
     4681 tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata cgatgacaaa
     4741 aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc cttggctgtt
     4801 aagaagcaat ttcaagagtg agcgaagcaa gagcactcct agagcagcag gcaaatccat
     4861 gaccaccaga cccacaagga ctggcaagat actaaattcg atcttgagga aagatgccgc
     4921 tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata gaattgcaat
     4981 ggtcgaaagt cgacgtgtgt ttgtcataat aggttcctcc aattttctat aaaatcagaa
     5041 gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag ttccctctta
     5101 ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta tcaaaacaga
     5161 caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg aatctcacca
     5221 catcagcttg cgctcgcgga cttctttaaa gagaaagaaa tagagatttt tcttttagac
     5281 taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca aagtataaaa
     5341 ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc tgccctgaag
     5401 actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat tttcattttt
     5461 tatttcaatt tatccacttc ataggtatgg acgagctcaa gacctgcaaa gttctgctgg
     5521 cgaagagcct catagacaat catgcagacg gtattagaca cattgagact gcggacatgt
     5581 tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa atcttcaggt
     5641 aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga taaatccacc
     5701 tcagaataga ttttttccgc gaatttcgaa atcagataga gttttccctt catctgagac
     5761 atgaaatctt ctaaactctc gtaaaaataa atctctagct tatcccagta atccaatcca
     5821 gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat gtggagggga
     5881 gaattggtcg cagcgcaagt acgcgcgata ttgcctgtat tttgtggaat ttgaggttca
     5941 aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa aaatagccac
     6001 actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact tacctcacaa
     6061 caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag aatcattgtc
     6121 aatagaaacg acttgatttt ttcaattttt tcaagctatt tccaagggtt gtaaaatcgt
     6181 ccctgattct gcaagataag tagtaaacta gctactaaaa acaaggttgc caagagcaag
     6241 gtaatatagt ctcctttttt caaggcctga taactatacc atgtgcgttt tttctctttc
     6301 ccaaagcggc gaagctccat ggcagtcgca atggtatcaa tgcgttctag cgagctaaaa
     6361 atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga agctttcttg
     6421 gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc ttcctgcaaa
     6481 tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg cacaccaatt
     6541 tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat aatagccaga
     6601 ggaatggtgc aaagatactt aatgaccaaa tttagcagat aaaagagctc ctggctggtt
     6661 agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag tccaacccca
     6721 tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatac cgtcgcaaaa
     6781 acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag aaagactgaa
     6841 aagatggcaa tcagcaagag cattctggta tcatagctaa tcatggccgc caatgatacc
     6901 agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt atctctatgc
     6961 tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat gccgttaaat
     7021 ccagtggatc cacatctagt ttcttagcca agttaaagat ggaggtttct tttagattgg
     7081 cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg gcaatcaatt
     7141 ctccatccac catgacaagg acccggtctg aataatccag catcaattgc atatcatggg
     7201 taatcatgac aatggtatgc cctttttgat gtaactcttc gagaaattcc ataatctcag
     7261 tatagttctt ctgatcttga cctgcagtcg gttcatctag gagaataatt tcagctccta
     7321 agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc aaatgacagg gcagaaatag
     7381 gccaattacg gaattcataa agtccacaga ttttcaaggt ttcatagact ctcgtttcaa
     7441 tttccttctc atccacacct cgcaaacgga gccctagagc cacctcatca aaaatcatat
     7501 tggttgaaat catttgatta ggattttgta gcacatagcc tactcgttcc gcccgctctg
     7561 caacagaatc gccttttata tcctgttttt cccaaagata gcgtccttcc gtctgaataa
     7621 agctacttat agccttggct agagttgatt tccctgctcc attttttccg acaatagcaa
     7681 tcttttcacc ctttttaata tctaaatgta gggattttaa aatcggtcta tcatcataag
     7741 agaaagacac gtcctctaat ttaaagagtg actgcaattc tggagtttct tttgccagtt
     7801 cattctgcag ctgaacctga ccttttgaga tagacatgtt atccagattc gctaactgtt
     7861 cttccttgac taagtccaca cctaattgac ggagagtcgt tagataaagg ggttctcgaa
     7921 ttccattttg agtcaataaa tcagtcgcaa gcaactggtc agggctccca ttaaaaagga
     7981 tacgaccatc gcttatcaag acaatccgat ccacagggcg atgcagaaca tcctccaaac
     8041 ggtgctcgat aatcagagtc gtcgtcccct cttccttatg aatctggtca atcaattcga
     8101 taatatcctg acctgacttg ggatctagat tggcgagtgg ctcatcaaac aagagaatcg
     8161 gactttcatc aatcaagaca ccagccagac tgactcgctg cttttgtcca cctgacaaat
     8221 cctgaggacg ctgatccagt aaaggaagaa ggtccagctt ttcagcccat ttataaacac
     8281 gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc aaatcttctg
     8341 ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta accagatgag
     8401 acttatcata gatgctcata tcaaaggcta cttgaccctt tatcaaaaat tctccatatg
     8461 tctgaccctt gtaaatattg ggaataatcc cattcaaaca ctgacccaag gtagatttac
     8521 ctgacccaga tggtccaaca attaagactt tctctccctt gtaaatggtc aagtctatcc
     8581 cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc cactcaatta
     8641 tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta tcataaatca
     8701 agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc tatcctagct
     8761 tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct tccccatcat
     8821 tttatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt aggtatggtt
     8881 actgacttcg tcagtttcat ctacaacctc aaaacactgt tttgagcaac ctgcggctag
     8941 cttcctagtt tgctctttga ttttcattga gtattagtcc tttttcaaac ttcctgcacg
     9001 agtttgggtt cctgcatagg caagtaagag aagagttcct gcaatagcta cagatacacc
     9061 attggcaatt cccgcaacaa tcccttgtgc aaatactttt tctgccgctt cttgataaat
     9121 cacaacatct ccaagtggtg ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat
     9181 gaggttaaaa ataagaatat ctttccagtc aaaaacacca ttgatcactc gaacgtactt
     9241 tctaaaaagt cccacaacta gaccaaagag tccgctagcg ataatccaag tccaccatag
     9301 accataacca gcaagagagt ccttgattgc atgaccaatc aacccgacaa gcaaaccgat
     9361 aatcggtcca aaaataatag aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt
     9421 atttggaaca ggggttggaa tgttgatcat cccgatgacg acaaagaggg cagcgccaat
     9481 tccgacagca acaacttgtt taattgtaaa tttgatttcc atactattct cctattttat
     9541 ccttccattt tctttatttc aatggtccaa gatgaaccga cacctacatt ataggccttg
     9601 gcaaaggaac cttggttgat agccaaacct aaacgataga gagagttgat gtaaaggatg
     9661 ggttgcccaa ttctcacatc tgcaaatgat ttgccataga caacctgatt ttgatagacc
     9721 agcatatcag catgatagat ggtcacttca aaacgatcac caaattctgg ttccagcttg
     9781 taaaattctt cccgtgtgat agaggtccaa agcgaaccga aacgcacatc cagaatatca
     9841 atggctccct tcaccagatg atcttctatg atggtcgcta cgactggaag ctctacaatc
     9901 tgttccacac tgagctctgg ccctacttcc tcaaaagtaa tgtgaccact ggccagttta
     9961 gcaccagtat aggcatagac atcacgaccg tggaaggtat aagaatgctc tgtgttttga
    10021 cgcctattgg ccacctcaga aatctcacga atggctacaa tgccaacgtg tttcttgata
    10081 aaggaaagcg tcccattatc tggcgtgaca atgtattgat tttttgcagt cttggcaact
    10141 acactcttac gtttcgaacc gacacctgga tcgacaaccg atacaaacgt cgttccctca
    10201 ggccagtaat ccaccgtctg aaagagacga tagctcccct caaaaatatt ataaggcgtg
    10261 atatcgtgcg tcaagtgatg gatttttaag gttggagact cttctaaagc cactccaatc
    10321 atagccgata ccgcaccatc aaccagacca aagtctgatt gtaataccag taaattatta
    10381 ttcattttat ctccttgtat atcctttatc ataccatatt tcagagaaag tcgctaatag
    10441 ctatttcaat tggttagggt atagttttac cttatagcga attttcacta aaagctcttg
    10501 ttattagcta gtaggtgcat tttttcttga aaaagggtat gatagttaca tcatgaaaaa
    10561 gtaggtttta ttatgaaaat tatccttgtc ggagggggaa aagttggttt tgccctctgt
    10621 cgctccttgg ttgcagaaaa gcatgatgtt ttgctgattg agcaagacga agctgttctc
    10681 aatcatattg tcagtcgctt tgatatcatt ggtatccttg gtaacggggc cgattttgcc
    10741 attcttgagc aagccagcgt ccaagattgt gatatcttta tcgccctgac tgagcacgat
    10801 gaagtcaaca tgattgcagc agttctagcc aagaaaatgg gagctaaaga aactatcgtt
    10861 cgggtgcgga accctgaata ttctaactct tatttcaagg aaaagaatat tctcggtttt
    10921 tctcttatcg ttaatcctga gctcttggct gcccgcgcta tcgcgaatat cattgacttc
    10981 cccaacgccc tgtctgtcga acgctttgct ggtggacgcg ttagcctcat ggaatttgtc
    11041 gtcaagtcca ccagcggtct ttgccaaatg cccatttctg attttcgtaa aaaatttggt
    11101 aatgtcattg tctgtgcgat agagagggat catcaaatta tcattccaag cggtgacatg
    11161 actgtacagg ataaagatag aatctttgtc actggtaacc gtgtcgatat gatactcttc
    11221 cataattatt ttaaatcacg cgccgtgaag agccttctca tcgttggggc aggtagaatt
    11281 gcctattatc tacttggtat tctcaaagat agtcgtatcg atacaaaagt cattgaaatc
    11341 aatcctgaaa tcgccagctt ctttagcgag aaattcccaa atctctacat cgttcaagga
    11401 gatggaaccg caaaagatat cctgctggaa gaaagtgctc aacactatga tgccgttgcg
    11461 actctaacag gagtcgatga ggaaaatctg attacatcta tgttccttga cagggtaggt
    11521 gtacagaaaa atattactaa ggtcaatcgt accagtctcc tcgagattat caatgcgcct
    11581 gatttttcaa gtatcatcac acctaaaagc atcgctgtag atacgattat gcactttatt
    11641 cgtggtcgag ttaatgccca gtattcagac cttcaagcca tgcaccatct agccaatggc
    11701 caaattgaaa ccctgcaatt ccatatcaag gaagccaata aaatgactgc caaacctctt
    11761 tctcaactga aattgaaaaa aggggttctt attgcagcca ttattcgaaa gggcaagact
    11821 attttcccaa ctggggagga tatgttggaa gttggagaca agctcctagt aacaaccttg
    11881 ttgccaaaca tcaccaagat ttatgacttg attgcgaggt aagaaatgaa taaaagtatg
    11941 attcgttacc tcctttcaaa gttacttttg attgaagctg ttctcctctt ggttcctgtg
    12001 gctatcgctg tctattaccg tgaatcgagc caagtcttta cagccctctt ttcgaccata
    12061 gggattctcg tattgttagg cggttcaggg agtttacaga agccaaaaaa tcaacggatt
    12121 tatgccaagg agggagtctt gatcgttgcc ctctgttgga tcctttggtc tttctttggt
    12181 ggtctcccct ttgtcttttc tgggcaaatc cctagcctta ttgatgcctt ttttgaaatt
    12241 agttctgggt ttacaactac tggagcaagt attttgaacg acatttcgat tctcagccgt
    12301 tccctcctct tctggcgaag ttttacccac ttgattggag ggatgggagt gcttgttttt
    12361 gcacttgcta ttatggacaa tgccaaaaat agtcacctag aagtaatgaa ggctgaggtt
    12421 cctggtcctg tctttggtaa ggttgtatcc aaattaaaaa acactgccca gatcctctat
    12481 ctcctttaca tagccctctt ttccctcttt gtcatcatct attatctagc cggtatgcct
    12541 ctctacgata gttttgtcat tgctatgggg acagcgggaa ctggaggatt taccgtctat
    12601 aacgatggaa ttgcccacta tggtagctca ctgattacct atctggttag tataggagtt
    12661 ttggtttttg gggtaaattt caatctctac tactacctta tgctccgtcg ggttaaggcc
    12721 ttctttggag atgaagagct tcgggcttac ttggtcatcg tgctggtttc tacaggcttg
    12781 attaccctca atactctcta cctctaccca gggttttcaa agagctttga aatgaccttc
    12841 ttccaggttt ccaacatcat tacaacaact ggttttggat atggagatat taccaactgg
    12901 cccctcttct cccagtttat cctccttttc ctcatggcaa tcggtggctc tgctggatca
    12961 actgcaggtg gactcaagat tattcgaggc ctcatccttt caaaaattgc caaaaaccaa
    13021 attttgtcaa tcctatcgcc ccaccgtgtt ttgaccctcc atgttaataa aatggtgatt
    13081 gacaaagata cccagcataa gattctcaag tactttgtca tctatgctat gattttgcta
    13141 gcgcttatct ttattgtcag cctagatagc aatgattttc tggtcgtgac cagcgctgtc
    13201 tttagctgtt tcaataatat cgggcctatt ctaggaacca cttctagttt ctcaatcttt
    13261 agtcccatct caaaaattct cctctccttt gcaatgattg cagggcgctt ggagatttac
    13321 ccaatcctcc ttctctttat gaaaagaact tggtctaaga gataaaatac aaaatccctc
    13381 tttttacaat tgtgaaagag ggatttttat attaaaaagg gggaacgttt ttaaatccaa
    13441 gcctgtctgt aagcaaagtt cttactcaaa taaaagatgg aaaaacttac tacacagctt
    13501 ggtaatgctc caatacaaat tgtaaggctt tacttggatt ctttgttaaa tgaatatatt
    13561 ccattcctct ggtagcaact atctgaatac ctaatctttc tgcatccacc ttcatctctc
    13621 tataataact ccgaacttgt ggagccagaa taattaaatc ataaacaccc ataatatcat
    13681 aatgagctcc gtacgctcct gaattcgcaa tcactctaac ttctgctaat tgagcgccct
    13741 cgttaattgc attggctaat tacgcacttg ttccagaccc tgcacaaaga accagtactt
    13801 tcaactcctt actttttatt acatctatct caccaggcat tatttcactt gtatcctcct
    13861 ctaaaataat atcatttgag cttgcaatat ctacaacctg tagtaaatat ctcgtattta
    13921 gacaatatga aaacaagacg acttccatat aggaaaccgc cttctcgcta tgttgcatga
    13981 aactgtaaaa gtggatgaaa ttgatagcgg tagtcagatc aagagacatc ataactctaa
    14041 aaagtcacaa tatataagtt catcctcgga aaaatatcat tctaattgtt gaaatggata
    14101 tataaacagg ggtattaaca ttaaatgctc atgaaacaac gaatacaggt atcaaaacta
    14161 tgacaaaaca agtccctaaa tttactaaag acactgctca actttacacc tgtaaatggt
    14221 tgttgtataa taaagttaca aagatgtacg accacactgt tgtaaatcat agtgttcgcg
    14281 aatatattac tgatagcatt tctacaaata caattgaaga gactggatag actttaaacg
    14341 aaatatgtca gtgttttggc attcctagtc ttcatatcat ttaaagaatt ctatagacaa
    14401 aattttttcc aatacagaca ctcgtaacaa ctgcttcatt tttctaccaa catatttagg
    14461 aacaggataa gatacaagag gattaatcca tagctcagtt ctataccaat ctaagacaaa
    14521 taagctaaaa aaacgattga taataagcaa atagattcca aattttctct atctgctcat
    14581 tttaataaac aatactagtg taactatcct tccagtcaga agcttgtcaa atcacaccga
    14641 aaactcttct aaaatttatc tcgttaggca atcaagcaaa aactcgacga tagtacaaac
    14701 attatcatac agaattgact tcctaaatta tatactttag taaggttttc ggataagaaa
    14761 aaaggttcat tttacatttc taaacattct tttctaagat gaaaaacaga atttttcgat
    14821 tgtgatttaa agcaacaaga agattttcag tatcatccta tagatacgag ctatttaaga
    14881 aaaactacat ttttgaatat aaactaaatt ttataggagg aagacaatgg attggtacga
    14941 ttatatgata caggcatcca aacaatcaca attcaacgca agccattggt ttcgctattt
    15001 gcgaaaagtt atttttgaag actattctta tttaacaaac caagatgtag aaaagttgct
    15061 agactccgaa gaactaaccc attttcaaaa aattagcttg aagtatgcct ttcaagagca
    15121 tactccaact cataaatatg tgatttcatt aaataaacct gctaagttaa ccaatgttca
    15181 aaaattgatg gagaaataca agcatggata aaatgaaacc ggtcttccaa gccctaaata
    15241 agaaattaat tcaggaaaat ctgactttaa caattatggt tatgtcttag aatatcatgg
    15301 tttacgtgcc acacaagatg ttgatgcttt ttatgaccaa aatcagaaaa tcaatgaaat
    15361 tattgctcgt gtaggaaaac agtttaatct taacatccac gaagagttat ggctcaacaa
    15421 tcatgttgca aagcaaatat gaacaaacaa cctcccttaa gtttatgcga atccttgtac
    15481 tcatttgaga atctgaccgt tttagtagtc cctattgagt atgtgttagg aatgaagatg
    15541 atgagtatca gagagcagga tttgaaagat attggtgcaa ttattaaata caaaaacttt
    15601 cattcaccat tcgatacctt taaatattta aaagacatgg gatttgatac tattgacctc
    15661 tctgttctat tagaaggttt tagttatgct tatggtatgg attggttaga aaaatttttt
    15721 aaagaaaatc aagataaact tcgcgaattt tattaacagt atcaaacaag atgattgtca
    15781 attagccagt cgtcttattt ttatgatgga atgaaataaa atcggaacaa atcacttaga
    15841 gaattccagt caatttctag cactgtttga gacgtttcag cgaactatag taaaatgaaa
    15901 taagaatagg acaaatcgat caggacagtc aaatcgattt ctaagaatgt tttagaagta
    15961 aaggtgtact attctaattt caatctacta tatttcagat tcggtccact attccttccc
    16021 aaaaacattg catattgcgc cgaaggaatt gctcatacta tcccaagcac aaaaccctca
    16081 gtcgtttgac cgagggttcc tttttattat tcaagaactt gattagctca atgcatccaa
    16141 ggcgtcatcc attgaaagaa cttcgtggaa gacacgttgt gtcaattcag ttttttgttc
    16201 tggagtgagg tatttagtgt ttacacagta tccagagata cgtacgataa cgtcttcgcc
    16261 tgacatgatt ttttcgtaaa catcgttcaa gtccataacg ttcaagttaa cgtgttgtcc
    16321 accgttttcg aagtaaccat caaggattgt taccaagtta tcaacttgtt catcacgagt
    16381 cttaccaaga gcgcgaggtg atacttgtgt agtcaatgag ataccgtcag ctgcataact
    16441 aaagtcaagg ctagaaagtg agttcaagtt ttgcaaccaa ccacctttag ctttgttaga
    16501 tgggttagca cctggtgaga agaattcaag tttagacaag ttcacagaac catcttcgtt
    16561 gaggtataca cctttgtgaa ctggtgagtt accagtttgt ttagagtaag caacgttaga
    16621 tgtgattgtc aaaagtgata ctgtagcttc tgcgtctttg tatagtttgt ggctacgtag
    16681 acgagttgtg taagcttcga ttaaccattc tgccaattcg tttgaacgtg ggtcatcttc
    16741 accccagcgt gggtagtcac cgattgtttc gtaatcgtag atgtagccat cttcgtcacg
    16801 gattggttta actgtagcgt atttgatagc tgacaatgta tcaacagtgt tagcaaatcc
    16861 acagataccg aatcccatgt tggcacgttg tttagttggc aagaaggcca tttgaacagc
    16921 ttcgtagttg tacctatcag tcatgtagtg gatgatgttc aaggcatcta cgtaagtgtc
    16981 agtcaaccag tcaagagatt tttcaaagtt cgctttaact gattcaaatt caagaacttc
    17041 gtcacggatt ggttcgatat caaatacttt gtagtctttg tgaacatcgt cgtaaccacc
    17101 attcaaacca gtaagaaggg ctttaagaac gtttacacga gcaccgaagt actggatgtt
    17161 gtggcgttgt tcttcatttt ctggatcaag tggagacaca cagcatgaga tacagctcat
    17221 ttcaccatat ccgtctttag ccattgttgt tacaccttcg tattggatag aagagtgttt
    17281 gtggctcatg tgcatacagt agcgacggaa gttgtatggc aatttgtcag tccaaagaac
    17341 tgtcaagttt ggttctggtg agttaccgat gttgtcaaga gtgttcaaga aacggtagtc
    17401 catcttagta acacggtgac gaccgtcgtt acccatacca gccatagaag ttgtgataaa
    17461 ggttgggtca cctgagtaca attggtcata agcttttgta cgagcaaatt taactgtacg
    17521 aagtttcata acgaaatcat caacgaattc ttggatttct gattcagtaa atgtaccacg
    17581 agcaaggtca cgttctgcaa agatgtccaa tacgattggt acacgaccta gagatgtagc
    17641 agcaccgtta atcacacggc agacagccat gaaagcaatg ttaacccatt ggattgcttc
    17701 tttcacgttc atcgctggtt tgcgaacatc aaccccgtaa aggtcaccca ggcgaacaac
    17761 ttgttgcaat gcttggtatt gaaggtttac ttcttcacga agacggattg tttcttcatc
    17821 gatttctttg attgcattcc agtcatttac tttttcttgc atcaagtagt ctgcaccgta
    17881 aagagcaaga cgtgcgtaaa caccgatgat acgtccgcgt gagtatgcat ctggaagacc
    17941 agttacagtg tgtgcgtgac gagcgcgacg aatatttgaa gtgtaggcac ggaaaatacc
    18001 gtcgttaact gttgttacat atttagtgaa gatttcgtga acagctgggt ctggttcgta
    18061 tccattttct ttcaaagtag tttcagccat acggatacca ccttttggca tgaagttcaa
    18121 tttgaagagt tcatcgtttt ggataccgaa gataacttca ttttctttgt cgataaatcc
    18181 agcagggata tcagcgatag atgttggacg agtgtccatt gggaaacgag tttcttcgta
    18241 gtgtgcttta gtttcttcta caattttctt gatgtgaagt gaacgctctg ttggtcctgc
    18301 aaggaagctt tcgtctccat cataaggtgt gtagttagct tgtacaaatc gtgatacact
    18361 tgctttttct ttccaatcta cgcctttgaa gccttcccaa gctttgtcaa aaatatcttg
    18421 tgcttcaaca actgtcttaa caaccatatt aatgtcctct tttttctagt aacagtcatc
    18481 tgttacattc ataagacaag tataccatat agtaaccgat ttcaacaagt gaaaattccc
    18541 tatttttaca ctttcttttc taaaacagtc tatattttat ttcaaactgt attatatttt
    18601 tgaaaaaata aagtcctttt ttcttttttt cagaaaaaag ggtataataa aagaaaataa
    18661 gcagtaacac tcaatggaaa tcgaaaagca aactaggaag ctagccgcag attgctcaaa
    18721 acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc gaagagtata
    18781 aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga aaaatcatcc
    18841 atattgacat ggatgccttt tttgctgcag tggaaatcag ggataatcct aaactcagag
    18901 gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga gtcgtttcta
    18961 cctgtagtta tgaggcaaga gcttttggtg tccattctgc catgagttcc aaggaagctt
    19021 atgaacgttg tccccaggct gtctttatct cagggaatta tgagaaatac aagtctgtgg
    19081 gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc atgagcattg
    19141 acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg gtcaaaattg
    19201 ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttcc gcaggcgttt
    19261 cttacaacaa attcttagct aaaatggcga gtgattatca aaaaccacat ggtttgacag
    19321 tgattctacc tgaacaggct gaggattttc tcaaacaaat ggatatttcc aaatttcatg
    19381 gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact ggtgctgatt
    19441 tacttgaagt tcctgaggta accctaatag accgttttgg tagactaggc tatgatctgt
    19501 atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcacatc cgtaaatcaa
    19561 tcggcaagga gaaaacctac gggaagattc tccgtgctga ggaagatatc aaaaaagagc
    19621 tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa gctggaaaaa
    19681 ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga aaaagtattg
    19741 ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc tatgaagaat
    19801 taagtgagaa agaaagaggt gtccgcctat tggggattac catgactgga ttttaaagct
    19861 caatgaaaat caaagagcaa actaggaggc tagccgcagg ctgctcaaaa cactgttttg
    19921 aggttgcaga taaagctgac gcggtttgaa gagattttct aagagtataa aaccttgaag
    19981 agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa agtagcaaaa
    20041 cactaccaag cacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt ttcacatagc
    20101 tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc gcgaccaaga
    20161 gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg aaaatcttta
    20221 aggcactagc tccaaacata acgggaatcc caagatagaa ggtaaattct gtcacaactg
    20281 aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg ctagtccctg
    20341 gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa ggaagcttgt
    20401 ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc aaatagatga
    20461 aggcaacccc gtagataatc aacatgagag caactgaaac catgttatgg aagtgggtat
    20521 caaaccaatc atcaaattta aagacaccaa gtaaaggcaa agtggcaatc aagaccttca
    20581 accatagtct ccaagtctta cgaacttcct gtttgtcctt agtcggttta aaaggattga
    20641 gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc acgacattaa
    20701 acatggacat aaaggcttca ttttgatttt ggtattggat aaactcctct gctaaaatca
    20761 agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatc ccgaagaaga
    20821 tagattttaa aatttcaata agatacatat atttctcctt atattatatt taacgcagtt
    20881 agctactgcc aaagcccaag tggtatactt gcaataagcc actgtggatt agttcatttc
    20941 ctccaatcgc ttgacgattt ccgaaagggt ctaaccacag ctttttgttt caattggtaa
    21001 tgagtttgat aacgcataga cgttttatct cgtctaaggt aacaaggcaa aaagttcgtg
    21061 gataaccaag tagacttgaa atactatatc tggaggtact cccatgcttt atgtgggcat
    21121 tgatgtcgct aaaaataaac acgatgttac agccttgaat gttcgaggaa aactgttctt
    21181 aaaccactca ctttttcaaa taataaagct ggttttgaac ttttagatct gtctcttcga
    21241 cagctcaacc aagactatct catcgctctt gaagataccg gacattatgc ctttaatcta
    21301 ctgaacttcc tgcatgaaca agaatataaa gtttacacct acaatccttt gctcatcaag
    21361 aaatttgcca aatcattatc acttcgcaag actaaaacgg ataagaaaga cgctcatggc
    21421 atcgctctta aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa
    21481 gtagaactaa aaatactggc tagacatatt catcgtctca agaaaaaaca gtctgattgg
    21541 aaagtacaat acactcgttg tcttgatatc atctttcctg agttggataa aatcattgga
    21601 aagcattcag aatataccta ccaactcttg acacgctacc ctaatcctca gaaaaggctt
    21661 gaggcaggat ttgataagct gatagaaatt aagcgattga ccgcttctaa aattcaggat
    21721 atcctctcag tcgcacctcg ttctatcgga acaacatctc ctgctcgtga attcgaaatc
    21781 atcgaaatca tcaaacatta caagaggctc attgacaagg cggaaacatg tgtcaatgac
    21841 ttgatggctg agttcaactc ggtcatcacg acggttactg ggattggggg tcgtttaggg
    21901 gcggtcattt tagccgagat tcgaaatatt catgcctttg ataatcctgc tcaattacaa
    21961 gctttcgctg gactggattc ttctatttat cagtcaggtc agattgattt agctggaaga
    22021 atgataaaac ggggttcccc tcatctgcgg tgggcactca tacaagctgc caaagcatgc
    22081 gctcgctttt cacctgcttt taaggcctat cttaagacta agttagaata agaaaaacat
    22141 tacaatgtag ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt ttatatccta
    22201 aaaaagagct gccatttgac gaacaaaaag tgatgtaatt tctgaaatca acacaggatt
    22261 acctttgaaa aaatggactt tttcaagggc tttttgccgt gctctaaaat catcgccaaa
    22321 tcatcaaatt gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc
    22381 ttttttctat cttccattat atcatacttt ttcagtctat ggtagctctc tttaaaaggc
    22441 accgatactg ccgccacctc cgcctccaga gaagccacca ccagaacttc cacttccaga
    22501 agatacggag taggtacttg ctgtatttgc gacactagca taatggctca tttgcgcgct
    22561 tgaatgataa aacatactgt gccagccata agctacatag agattgatat ctggattttc
    22621 aacttgaatc tgatgaacct tcatcaaatg actaaccttg tccgcatagc caaataaggt
    22681 tgcatagacc aagagacgat tccaaacaac aatactttcc aattcagcct tatccagtcg
    22741 tgcaatctca cgtaacatat tttcaaaact ggtccagaga tagtagacct cagctcccgc
    22801 ttcatttaga acaccatcac gattatctag tcgaagcttc caataataga aaacagacaa
    22861 aactaaccct agaaaaccaa gtattggcaa agggaggtaa agatagccat gaacgtctaa
    22921 actgtacaag aacaaaccaa atccgataaa taggggcaag atagtcaaga cacccatacc
    22981 cacttgcaaa gccttttcca aaccagttaa aggacgatag taatctggga gcccccagaa
    23041 ggaaactcgt tttctcactc cttcttgcat ctggttcaat acctcttcaa aagaagattt
    23101 gagttgaagc cctcttgctt gaatccgttt ttcatcagaa actttggctc tacgataaag
    23161 actatcagat accttgtaat ccgcaaacaa attggaaaga gtttcttctt ttttacctga
    23221 aaaagctaga tttaggcagt ctttctcaaa gcttgacaaa ccatcttctt ttactagcct
    23281 caaaccaact gcatctcctt ctgaaatgat agagacattc ccacggtcta tcacatctag
    23341 caaggtagct tgaataagtt gatcaaaggt gaattttcca gctcccttga ccaagggact
    23401 cacttcctcc aaggaggtcg agtagactgc ttctgataaa accataggct ctaattccat
    23461 tgggggttca tagagacgat gatttttagc atatttgatt gaaggagcgg tctttcttct
    23521 atagataaaa tagaaacaga tgctcaataa caaggagatg gaaaggatcg aagggaagat
    23581 ccaagtaagt agttgtttac tttgatcttt ttctttaacg atcgaatcct ctatcttatt
    23641 aaactcttct aaacgattcc ctttcaagcc ctgatcccta gcgctagcaa aatcagttcg
    23701 cggccagtag gcatgcaatt caactccacg cttagccgga agattgtcta aacgaatagt
    23761 ataatcaagg ttactctttt caaccgttct ctctctaaaa agtttccctg tatggaaaaa
    23821 gagtttctca gcccccttgt ctccccttac atgaaattca aacttttcaa tagactctga
    23881 actatctgtc agaggttgcc aatttaattc agcgatatca tcataaagga aaagtaaatt
    23941 ttttaagttc cagacgaggt caacttcaac tatgtcgccc tcctgacctg gattatagac
    24001 tctcacagta taaccatccg cttcttctgt tacttcgcta gtcacatctg ctagttctgc
    24061 accgtttttc gcggcctgaa tctttggatg agggtcaatg tcaaacccgc taggcatctt
    24121 accagcacgt ccaagtccca cgatttggcc cttaaagtcc tcctcaaact ggtaaactat
    24181 cttctgtcta aactctgccg tattgtctgc atgaatatac aaatcccctt gataagagtt
    24241 tatcttgaaa tcaatggcaa aaacagagag tggaagaagg caaaacaagc ctaacaccag
    24301 taagaaaaaa gtttttttca tcaactaagc ccccttttta tctttcttat cattatagca
    24361 ttttttcgta agtaaaggct tacttatgtt gaaaaattac gcatttttcg ataaaatagg
    24421 agacagttat tttttaatag atcagaatag gagaaatcat gagaaaaata tacttatcta
    24481 ttttcacaag tctcttgctg atgctaggac ttgtcaatgt tgctcaagcc gatgaatatt
    24541 tacgcatcgg tatggaagca gcatatgctc cctttaactg gacccaggat gatgatagca
    24601 acggagctgt caaaatcgat gggaccaatc agtatgccaa cggatacgat gttcaaatcg
    24661 ccaagaaaat cgctaaggac ttaggtaaag aacctttggt tgttaaaacc aagtgggaag
    24721 gtctagtccc tgcccttact tctggtaaga ttgacatgat tatcgcaggt atgagtccaa
    24781 ctgcagaacg caaacaagaa attgcctttt cgagcagtta ctatactagc gaaccagttt
    24841 tgcttgtcaa aaaagattct gcctacgcaa gtgctaaatc tttggatgac tttaacggtg
    24901 caaaaatcac ttctcaacaa ggggtctacc tttataactt gattgcacaa atcccaggtg
    24961 ctaaaaaaga aacagccatg ggagacttcg ctcaaatgcg acaagctctt gaggctggtg
    25021 tcattgatgc ttatgtttct gaacgtccag aagcactgac tgctgaagct gcgaactcta
    25081 agttcaagat gattcaagta gaacctggtt tcaaaactgg ggaagaagat acagctatcg
    25141 ctatcgggct tcgtaaaaat gacaatcgta ttagccaaat caatgccagc attgaaacca
    25201 tttcaaaaga tgaccaagtt gccttgatgg atcgtatgat caaggaacaa cctgccgaag
    25261 ctacaacaac tgaagagact agcagtagtt tctttagcca agttgctaaa attctttctg
    25321 aaaactggca acaactcttg cgtggtgctg gtatcactct tttaatctct atcgtcggaa
    25381 ccatcatagg tctcattatt ggacttgcca ttggtgtctt ccgtactgct cctctctctg
    25441 aaaacaaagt catttacggc ctacaaaaac tagtcggctg ggttctcaat gtctacattg
    25501 aaattttccg tggtacgcca atgattgttc aatcgatggt tatctactat ggaactgccc
    25561 aagctttcgg gatcaacctt gaccgtacac tggctgctat cttcatcgtt tcaatcaata
    25621 ccggtgccta catgactgaa atcgtccgtg gtggtatcct agcagttgac aagggacaat
    25681 ttgaagctgc gactgctctt ggtatgaccc ataaccagac catgcgtaag attgtcctac
    25741 ctcaggtagt ccgtaacatc ctacctgcaa ctggtaatga atttgtcatc aatatcaaag
    25801 atacatctgt attgaacgtt atctctgttg tcgaacttta tttctcagga aataccgtgg
    25861 caacacaaac ctatcaatac ttccagacat ttacaatcat cgccgtgatt tactttgtcc
    25921 tcaccttcac cgtaacacgt atcctacgct ttatcgagcg cagaatggac atggatacct
    25981 acactacagg tgctaaccaa atgcaaacgg aggatttgaa ataatgacac aagcaatcct
    26041 tgaaattaaa cacctcaaaa aatcctatgg acaaaacgaa gtgctaaaag acatttcact
    26101 cactgtccac aagggagagg tcatctctat catcggaagc tctggaagcg gaaaatcgac
    26161 cttcctacgc tccattaacc tacttgaaac accaactgat ggacaaatcc tttatcatgg
    26221 acaaaacgtc ctcgaaaaag gctatgacct cacgcaatac cgtgaaaagt tggggatggt
    26281 tttccaatcc tttaacctct ttgaaaatct caatgttctt gaaaacacaa tcgtcgctca
    26341 gacaactgtc ctaaaacgcg aacgcacaga agctgaaaag attgccaaag aaaacctgga
    26401 aaaggtcggc atgggagaac gctactggca agccaaacca aaacaactct caggtggtca
    26461 aaaacaacgt gtggccatcg ctcgtgccct ctccatgaat ccggacgcta ttctctttga
    26521 tgaaccaaca tcagctctcg atccagaaat ggttggagaa gtcctcaaaa tcatgcagga
    26581 cctggctcag gaaggcttga ccatgattgt cgtaacccat gaaatggaat ttgcccgtga
    26641 tgtctctcac cgtgttatct ttatggataa gggcgtgatc gctgaagaag gtaaaccaga
    26701 agacctcttt accaatccta aagaagaccg aacaaaagag ttccttcaac gctatctcaa
    26761 ataaaaagaa aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc
    26821 agattctcta tctctactac ttgaaggtca agaatccgcc gttatccaaa gattaatctt
    26881 caaatttttc ataatttttt tcatagaaat caattaggcc tagggctgtt tcaaatgaaa
    26941 tattttttac ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg
    27001 tttctttaga gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt
    27061 ctactttttc aaagtttgac ttagccatgt tttt
//
LOCUS       DAVGJJ010000019        26765 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000019 DAVGJJ010000000
VERSION     DAVGJJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.019"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            65..3862
                     /gene="spuA"
                     /locus_tag="VVE35_000743"
     CDS             65..3862
                     /gene="spuA"
                     /locus_tag="VVE35_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU3060977.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDINDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPVTPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(3962..5011)
                     /locus_tag="VVE35_000744"
     CDS             complement(3962..5011)
                     /locus_tag="VVE35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU3060978.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            5068..5145
                     /locus_tag="VVE35_000745"
                     /pseudo
     CDS             5068..5145
                     /locus_tag="VVE35_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5283..7091)
                     /gene="glmS"
                     /locus_tag="VVE35_000746"
     CDS             complement(5283..7091)
                     /gene="glmS"
                     /locus_tag="VVE35_000746"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU3060979.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7283..8662)
                     /gene="bglA"
                     /locus_tag="VVE35_000747"
     CDS             complement(7283..8662)
                     /gene="bglA"
                     /locus_tag="VVE35_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU3060980.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSEKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8762..10615)
                     /locus_tag="VVE35_000748"
     CDS             complement(8762..10615)
                     /locus_tag="VVE35_000748"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU3060981.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10628..11887)
                     /gene="rseP"
                     /locus_tag="VVE35_000749"
     CDS             complement(10628..11887)
                     /gene="rseP"
                     /locus_tag="VVE35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU3060982.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLGQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11909..12712)
                     /locus_tag="VVE35_000750"
     CDS             complement(11909..12712)
                     /locus_tag="VVE35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU3060983.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLTMHE
                     LLKMRGLETMTMEGLLTLFATFVLTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12721..13479)
                     /locus_tag="VVE35_000751"
     CDS             complement(12721..13479)
                     /locus_tag="VVE35_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU3060984.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13709..14281)
                     /locus_tag="VVE35_000752"
     CDS             complement(13709..14281)
                     /locus_tag="VVE35_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU3060985.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14262..15233)
                     /gene="ruvB"
                     /locus_tag="VVE35_000753"
     CDS             complement(14262..15233)
                     /gene="ruvB"
                     /locus_tag="VVE35_000753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU3060986.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15394..15486
                     /locus_tag="VVE35_000754"
     CDS             15394..15486
                     /locus_tag="VVE35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU3060987.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(16113..16529)
                     /locus_tag="VVE35_000755"
     CDS             complement(16113..16529)
                     /locus_tag="VVE35_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060988.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(16541..17236)
                     /locus_tag="VVE35_000756"
     CDS             complement(16541..17236)
                     /locus_tag="VVE35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3060989.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(17431..19932)
                     /locus_tag="VVE35_000757"
     CDS             complement(17431..19932)
                     /locus_tag="VVE35_000757"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU3060990.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDTITSPEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAYLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDTSIAWSEEGLEGSRKFLDRVYRLITSKEIVAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEEKLYVDYAKGFIQMIAPFAPHL
                     AEELWQTVAATGDSISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            complement(20387..21475)
                     /locus_tag="VVE35_000758"
     CDS             complement(20387..21475)
                     /locus_tag="VVE35_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU3060991.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(21493..22161)
                     /locus_tag="VVE35_000759"
     CDS             complement(21493..22161)
                     /locus_tag="VVE35_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU3060992.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAREIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(22180..24618)
                     /locus_tag="VVE35_000760"
     CDS             complement(22180..24618)
                     /locus_tag="VVE35_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU3060993.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(24769..26067)
                     /locus_tag="VVE35_000761"
     CDS             complement(24769..26067)
                     /locus_tag="VVE35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3060994.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGTIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(26084..26392)
                     /locus_tag="VVE35_000762"
     CDS             complement(26084..26392)
                     /locus_tag="VVE35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3060995.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(26438..26758)
                     /locus_tag="VVE35_000763"
     CDS             complement(26438..26758)
                     /locus_tag="VVE35_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3060996.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         8150 a         6089 c         4838 g         7688 t
ORIGIN      
        1 acgcttgcat attgttttac tacatgctat actgatatag aaatttattg attggagaat
       61 cattatgaga aaaacaccat ctcacactga gaaaaaaatg gtctacagca tccgttccct
      121 caaaaatgga actggttctg tccttatcgg cgcaagcctt gtcttgcttg ccatggctac
      181 accaactatc tcatccgacg aaagtacacc aaccactaac gaacccaaca acagaaatac
      241 aaccaccctt gcccaacctc ttactgatac agcagctggc tctggtaaga acgaaagtga
      301 tatttcttca cctagaaatg caaacgcttc cctagagaaa acagaagaaa aacctgcaac
      361 agagccaact acttctacta gtccagtaac aactgaaact aaggcagaag agcccatcga
      421 agataactac ttccgtatcc atgtcaaaaa acttcctgaa gaaaacaagg atgctcaagg
      481 actatggact tgggacgatg ttgaaaaacc atctgaaaac tggccaaacg gagctttgtc
      541 cttcaaggat gccaagaaag atgactacgg ctattaccta gatgtcaaat taaagggaga
      601 acaagccaag aaaattagct tcctcatcaa caatacagct ggaaaaaatc taaccggcga
      661 taaatctgta gaaaaactag ttccaaaaat gaacgaggct tggttagacc aagattacaa
      721 ggttttctct tacgagccac agcctgcagg aactgttcgc gtcaactact accgcacaga
      781 tggcaactac gacaagaaat ctctctggta ctggggagat gtaaaaaatc caagtagcgc
      841 tcaatggcct gacggaacag actttacggc tacaggcaaa tatggccgct atatcgacat
      901 tcctcttaat gaagccgcaa gagaatttgg atttttatta ctagatgaga gcaaacaagg
      961 agacgacgtg aaaatccgta aagaaaatta taagttcaca gatttgaaaa atcatagcca
     1021 aattttccta aaagacgatg atgaatcgat ttacacaaat ccatactatg tccatgatat
     1081 ccgtatgaca ggagcccaac acgtaggcac ttctagcatt gaaagtagct tttcaacact
     1141 tgtcggtgct aaaaaagaag atatcctcaa acactccaac atcactaatc acctaggaaa
     1201 caaggtaact attaccgatg ttgcaatcga tgaagctggt aagaaagtga cctacagcgg
     1261 agatttctct gacacaaaac atccttatac tgttagctac aattccgacc aattcactac
     1321 caaaacaagc tggcgcctga aagatgagac atacagctat gatggcaaac tgggagctga
     1381 cctaaaagaa gaaggaaaac aagttgattt gaccctttgg tcaccaagtg ctgataaggt
     1441 ttctgttgtt gtctacgaca agaatgaccc tgacaaagta gttggaactg tcgctctcga
     1501 aaaaggggaa agaggaactt ggaaacaaac tctagacagc acaaacaaac tcggaatcac
     1561 agatttcact ggctactatt atcaatacca aatcgagcgt caaggtaaaa ctgttcttgc
     1621 actcgatcct tacgctaaat ctcttgctgc ttggaatagc gacgatgcca agattgacga
     1681 tgcccataaa gtggctaaag ccgcctttgt agatccagct aaactcggac ctcaagactt
     1741 gacttatggt aagattcaca atttcaagac tcgtgaagac gccgttatct acgaagctca
     1801 tgtgcgtgac ttcacttcag atcctgccat tgcaaaagac ttgaccaaac catttgggac
     1861 ttttgaagcc ttcattgaaa aactagacta tctcaaagac ttgggtgtaa cccatatcca
     1921 gctccttcca gtcttgtctt actactttgt caatgaattg aaaaaccatg aacgcttgtc
     1981 tgactacgct tcaagcaaca gcaactacaa ctggggatat gaccctcaaa actacttctc
     2041 cttgactggt atgtactcaa gcgatcctaa gaatccagaa aaacgaatcg cagaatttaa
     2101 aaacctcatc aacgaaatcc acaaacgtgg tatgggagct atcctagatg tcgtttataa
     2161 ccacacagcc aaggtcgata tcttcgaaga cctagagcca aactactacc actttatgga
     2221 tgccgatggc acacctcgaa ctagctttgg tggtggacgc ttgggaacaa ctcaccatat
     2281 gaccaaacgg ctcctagttg actctatcaa atacctagtt gatacctaca aagtggatgg
     2341 attccgtttc gatatgatgg gagaccatga cgccgcttct atcgaagaag cttacaaggc
     2401 tgcacgcgcc ctcaatccaa atctaatcat gttaggcgaa ggttggagaa cctatgccgg
     2461 tgatgaaaac atgcctacta aagctgctga ccaagattgg atgaaacata ccgatactgt
     2521 cgctgtcttt tcagatgaca tccgtaacaa cctcaaatct ggttatccaa acgaaggtca
     2581 acctgccttt atcacaggtg gcaagcgtga tgtcaacacc atctttaaaa atctcattgc
     2641 tcaaccaact aactttgaag ctgacagccc tggagatgtc atccaataca tcgcagccca
     2701 tgataacttg accctctttg acatcattgc ccagtctatc aaaaaagacc caagcaaggc
     2761 tgagaactat gctgaaatcc accgtcgttt acgacttgga aatctcatgg tcttgacagc
     2821 tcaaggaact ccatttatcc actccggtca ggaatatgga cgtactaaac aattccgtga
     2881 cccagcctac aagactccag tagcagagga taaggttcca aacaaatctc acttgttgcg
     2941 tgataaggac ggcaacccat ttgactatcc ttacttcatc catgactctt acgattctag
     3001 tgatgcagtc aacaagtttg actggactaa ggctacagat ggtaaagctt atcctgaaaa
     3061 tgtcaagagc cgtgactata tgaaaggttt gattgccctt cgtcaatcta cagatgcctt
     3121 ccgacttaag agtcttcaag atatcaacga ccgtgtccac ctcatcactg tcccaggcca
     3181 aaatggtgtg gaaaaagagg atgtagtgat tggctaccaa atcactgctc caaacggcga
     3241 tatctacgca gtctttgtca atgcggatga aaaagctcgc gaatttaatt tgggaactgc
     3301 ctttgcacat ctaagaaatg cggaagtttt ggcagatgaa aaccaagcag gatcagtcgg
     3361 aattgccaac ccgaaaggac ttgaatggac tgaaaaaggc ttgaaattga atgcccttac
     3421 agctactgtt cttcgagtct ctcaaaatgg aactagccat gagtcaactg cagaagagaa
     3481 accagactca accccttcca agcctgaaca tcaaaatgaa gcttctcacc ctgcacatca
     3541 agacccagct ccagaagcta gacctgattc tactaaacca gatgccaaag tagctgatgc
     3601 ggaaaataaa cctagccaag ctacagctga ttcacaagct gaacaaccag cacaagaagc
     3661 acaagcatca tctgtaaaag aagcggttcg aaacgaatcg gtagaaaact ctagcaagga
     3721 aaatatacct gtaaccccag ataaacaagc tgaacttcca aatacaggaa tcaaaaacga
     3781 aaacaaactc ctatttgcag gaatcagcct ccttgcgctc cttggtctcg gtttcttact
     3841 aaaaaataaa aaagagaact aaactagccc tcctatagaa aaatccccca agcattatag
     3901 ctcgggggat taattttcgt acaatatttg ttgtcctaat aaacttgatt aggatttttt
     3961 attaagcctc tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga
     4021 gttcaatagc tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga
     4081 agcggtccaa tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat
     4141 tgccaacaaa catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt
     4201 cctgccagtg cggacggtct ttggaaatag catccaccac ttgcttagtc ggatggaaat
     4261 aatctttcac cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt
     4321 tcaagtcttt gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact
     4381 ttttaaaata acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag
     4441 caatcttcac tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct
     4501 gaactggacg aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt
     4561 ctaacttggt cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt
     4621 cgtagtcttt caagtcataa ccaaacagag ggaaagattc cgtgaaagag ccccttccag
     4681 ccataatctc cgatcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac
     4741 gaatcggatc catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat
     4801 tgactgcccc agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt
     4861 gctcgccaat cccatatacg tccaaaccaa ccttgtcagc cagctcaatc tctgccacca
     4921 actggcgaat gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt
     4981 ccccaaatgt tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtactt
     5041 caatttgaaa aattattcta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     5101 gcaggctgct caaaacagtg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
     5161 acctacggca aggcgacgtt gacgtggttt gaagagattt tcgaagagta taacacgaat
     5221 cttgagtaca agcaaccaat ttgctcacaa gaaaaaggcc tagataacta gactttttta
     5281 gcttattcta ccgttactga cttggcaagg ttacgtggtt tgtccacatc gaggccacgg
     5341 tggagggttg caaagtaagc gactaattgc gttggtacga ccattgaaat tggtgagagg
     5401 tatggatgta cggtcgtaag gacgatatcg tcggtatctt tggctacatt ctcttctgcg
     5461 atagtgagga ctttggcacc acgggctgcg acctcttgga tatttccacg agtatggttg
     5521 gcaagaactg gatctgacaa gagagacaag acaggcgttc cttcttcaat caaggcaatg
     5581 gttccgtgct tgagttctcc tgccgcaaaa ccttcacact ggatataaga aatctctttg
     5641 agtttgagac ttgcttccat ggctacgtag taatcttgac cacgtccgat gtaaaaggcg
     5701 ttacgagttg tttcaagaag ttcacgaacc ttggcttcaa tggtttcttt ctctgaaaga
     5761 gttgattcga tagactgagc tacgattgac aattcatgaa ccaggtcaaa ggcttgcgct
     5821 ttagcattac catttgcttc tccgactgct tttgcaagga aggcaagggc tgcgatttgc
     5881 gctgtatagg ctttagttga tgccacggca atttcaggac ctgcgtgaag gagcatggta
     5941 tagttggctt cacgtgagag ggttgaacct ggaacgtttg tcactgttaa gcttggaatt
     6001 cccatttcat tagccttgac caaaacttga cgactatccg ctgtttcacc agattggctg
     6061 ataaagatga agagtggttt cttgctgaga agtggcatac cgtagcccca ctcagatgag
     6121 attccaagtt caactggtgt atctgtcaat tcttccaaca ttttcttaga agcaaatcct
     6181 gcatggtaag atgttccagc tgcaaggatg tagatgcggt ctgcgtcttg aacagcctta
     6241 atgatagcag gatcaatcac tacttgacca gcatcatccg tgtaggcttg aatgagttta
     6301 cgcataacag ttggttgctc atcaatttcc ttaagcatgt agtaaggata agttccctta
     6361 ccgatatctg acaagtcaag ttccgcagta tagctagcac gttcacgact gttaccatca
     6421 tagtcttgaa cttccacgct atcagccttg acgattacca actcttggtc atgaatttcc
     6481 atgtattggt tggtttcacg aatcatagcc atagcatctg agcagaccat attgtagcct
     6541 tccccaagac caatcaaaag tggagatttg tttttcgcta catagatgac atctggattt
     6601 tcagagtcaa tcaaggcaaa ggcatatgaa ccacggataa tatgaagagc ttttttaaag
     6661 gcttcaagaa ctgagagacc gtcttcttcc gcaaattttc caatcaaatg tacggcgatt
     6721 tccgtatctg tttgcccttt gaagtggtgc ccagcaaggt attcttcttt aatttcaagg
     6781 tagttttcaa tcaccccatt gtgaaccaag acaaaacgtt ctgtctcaga gcggtgtggg
     6841 tgagcattgt cttccgttgg tttcccatga gttgcccaac gagtatgtcc gataccagtt
     6901 gttccctcaa caccagctgt cttggcagac aattctgcga tacgaccaac agccttcacc
     6961 aaatggttat cagcaccatc taggacaaaa attcccgcag aatcatagcc acggtattca
     7021 agcttttcaa gcccttgaat caaaatatca gttgcatttg tgtttccaac aacaccaaca
     7081 attccacaca tagtatatac gacacaggca agctgtgctt tctccttaaa attggtatag
     7141 tctaattcat cttttataga atcagcaaaa acagtatata cttgtttctt tcacttgtca
     7201 agagtaaaaa ttggtatagt tcaaattaag ctcctgtaag caaaaaactc tgaccgattg
     7261 ggataatcag tcagagtcct ttttaaaatc cattattatc gcttaattct ttgaaccagt
     7321 ggcctgattt cttcagacga cgttcttgcg tttccaaatc taattcgacc aaaccatagc
     7381 gatttttata gctgttgagc catgaccagc agtcaataaa ggtccaaatc aagtagccct
     7441 tacagttggc accatcttca atggcacggt gaagttcacg aagatgacct tttacaaagt
     7501 caatacggta atcatcttga atcattccat cttgacggaa tttttcttcc ccttcaacac
     7561 ccataccatt ctcagtcaac atccattcaa tattgccata attttccttg atattttggg
     7621 cgatgtcata aatcccttgc tcataaatct cccaaccacg gtgagaattg attttacgtc
     7681 caggcatcac ataaggctcg taaaaatgtt ctggtaagag tggactctct ggatgcttag
     7741 caaatcgagg agccataaca cgcaaaggtt gatagtagtt cacaccaagg aagtccaccg
     7801 tattatcacg aatgagttcc aactcttcct ctgtagcatc aggtaaaaga ccgtgttcat
     7861 gcaagatttc taccaactcc tgtggataag tccccaagac agatggatct aagaaagatt
     7921 gggcctgaaa aagggccgca atacgagctg ccttgacatc agcaggatgc tggctacgtg
     7981 gataagccgg tgtcaagttg aggacaatcc caatcttgga atcaggcaaa agttcatggc
     8041 aagccttaac agcccggctg ctggccaatt gtgtatgata ggctacctta acagctgcct
     8101 ctgcatccac cttatgtgga taatgggcat cataaaaata accaaattct acaggaacga
     8161 tgggctcgtt aaaggtaatc cattgatcca ctaaatctcc ataagtctca aaacaaaaac
     8221 gagcatagtc ttcataggct gagactgtcg ccttattttc ccaaccatca ccatcctctt
     8281 gaagggcaaa aggtaaatca aaatgataga gattgactaa cagacgaatt cctttagcct
     8341 taatagcctc aaagacctta cgataaaaat ccacaccttg agggttgact tttccacagc
     8401 cttgtggaaa aatccgtgac cactgaatag aagttcgaaa gactgtgtga ccagtctcta
     8461 acaaaagctc aatatcccgc tcccaatttt cataaaaagt cgatgtcttc tctgaaccaa
     8521 tcccattata gtaacgattt ggctccactt ggaaccagta atcccagaga ttgtctccct
     8581 taccgtcacc agctacacgt ccttctgtct gcggtccaga agtagaggat ccccagacaa
     8641 aatcctttgg aaatcttagc ataacattta cctctttatc tactcatttc tcccattata
     8701 cagaaaaaac aaggtaaaaa ctagttacat tttttccttg tttttcttct gattatagtt
     8761 tttatttctt gcttaggatt tcaagcgttt caagcacgtt atctgcatga acctcaatgg
     8821 tgtcaccagt tgccttgatc ttaacttcta caatgccatc ggccgctttt ttaccaacag
     8881 tgatacggat tggaagacca atcaagtcac tatcgctaaa tttaacaccg acacgttcgt
     8941 tacggtcatc tgtcaagact tcataaccag ctcccatcaa gcttgcttca agtttttctg
     9001 tcaaggcttg cgcttcttca tccttgacat tgacagtaat caaatgcaca tcaaatggtg
     9061 ccaattcttt agggaaattg attccccaag cgtaacggta ttcacctttt ggcgttttgt
     9121 taacaaagag gcgagcgtgt tgctccatca ctgctgaaag aagacggctg acaccgatac
     9181 cgtaacatcc catgatgatt ggcacagcac gaccattttc atccaagaca tctgctccca
     9241 tgcttgctga gtagcgagtt ccgagtttga aaatatgacc gatctcaata ccacgcgcaa
     9301 agttaaggac accttgtcca tctggggaaa tttcaccctc acgaacttca cggatatcca
     9361 catattctgc agtaaaatca cggcctgggt tcacaccagt caagtggtag ccatcttcgt
     9421 tagcaccgac aactgcattg cgaacatctt gcaccttacg atctgcaata attttaatat
     9481 tctctggcaa accaactggt ccaagtgaac caaatcctgc ttgaacaaca ttcgccactt
     9541 cttcttcgct agcaacgtca aagaaatctg ctcccaagtg atttttcaac ttgacttcgt
     9601 tgagttggtc atttccaact agaagggctg caacaagctc gccatctgca atgtagaaga
     9661 gggttttaat cgtttgttct tctggaacat tgaggaaggc tgcaacttca tcaattgatt
     9721 taacatctgg cgttgcaaca cgagtaactt cttcttcagc gacaacacgg ttgcttggtt
     9781 tgtactcgtt tgttgccatt tctaagttag ctgcatagct agactcactt gagtaagcaa
     9841 tggtatcttc accagagact atccatttga gcaattctgc cttgatttct tcttgcactt
     9901 ctgcaggaat ttcgtcaaat gaggcaactg acttgtccaa gacaacccag cggtcaaggt
     9961 ctgtacgagc agatgtaatg gccataaatt cttggctatc cttaccaccc atggctccac
    10021 cgtcaccaat aatagccttg aagtctaaac cactacgagt gaaaatacgc tcataggctg
    10081 ctttgtactc atcataaaca ctatccaaac tatcatagtt agcgtggaaa ctataagcat
    10141 ccttcatgat aaactcacgt gtacgaagaa gtccattacg tgggcgtttt tcatcacgat
    10201 acttgggctg aatttgataa aggttgagtg gcaattgctt gtaagattta acagaatcac
    10261 ggacaatagc tgtaaaggtt tcttcgtgag ttggacctaa gataaagtct gatttttcac
    10321 ggttttttag tttgtaaagg tcttcaccat aggtttcgta acgacctgat tcacgccaca
    10381 attctgcact aagaagggca ggagccaaca tctcaacagc accaatcttt tcgaattctt
    10441 ggcgcatgat gtttttagct ttttcaatca cacggttggc aagtggtaga taagaataaa
    10501 cacctgctga aacttggcga acataaccag cacgcaacat aagagcatgg ctgataactt
    10561 gagcatcgct tggcatttcg cgaagcgttg ggataggcat tttactttgt ttcataatat
    10621 tcctcgatta tctaaaaaag agtcgcataa tgtcattcca agtcacagca atcatcaaga
    10681 caaccatgat gaccactccg gccaaggtga cataggtttc aatttcttgt ttcaatggtt
    10741 tgcggcggat ggcttctagg atattgagca caatcttacc accatccaag gctggaatcg
    10801 gaataagatt aaaaatccca atattgatgg aaatcattgc caagaagtac aagatattct
    10861 caattccatt tttagcagca tcactacttg ccttaaagat agcaacaggt ccacccaact
    10921 tgttcaaatc cggttggaaa atcagatttt tcagagctga gagaattcgg agagctgagt
    10981 cagcagcagt tgtaaaacca cctacaaaca tggatagaaa atctgactta acccccggtt
    11041 gaacacctag aaggtaacga ccttgactat cttcgggtgt aacagtgact tgtttgtcac
    11101 tccccttttc agaaatagtc acatccaaag tcggtgccgt cttatctttg gtttctgttt
    11161 ccacagcttg gatcaagctt tcccagttgc taacctcatg tgagccaatc ttggtaattt
    11221 gtgccgtttc tggtactcct accttggcca aggcaccttg gggcatgata tggaactgat
    11281 tggtatcaac atctctgaca ccaccctgca taaagattaa aacccaaaaa acaacaacac
    11341 ctaagataaa attgttcata ggacctgcaa aattggtaat cagtttgccc cagatagtcg
    11401 cattttgata ttgaacatct aaaggtgcaa tccgaacctc agtaccatct gcttccacaa
    11461 ccgttgcatc gtgatccact gcaaatgttt tttcttcttc cagaaccaat cctttgataa
    11521 agagcttgtc ttcaaaatca aactgggtca cctgcatagg gagggctgtt tgacccaatt
    11581 ttttacctga gagattgatg cgtttaacct taccatcatc agcaagtgtc aaactaacag
    11641 gcgttcctgt cttgatttca gttgtatcat caccccaacc ggccatgcgg acatagccac
    11701 ccagaggcaa gattcgaatg gtataggccg ttccatcctt gccaatgtga gcaaagattt
    11761 taggtcccat accgatggca aattcacgta ctaaaatccc tgatttcttg gcaaagtaga
    11821 agtgcccgaa ctcgtgcacc actacaataa tcccaaaaac cagaataaag gttaaaattc
    11881 cgagcatagc gtttcctccg tcttttgatt aaaagagtcc aaataagtgc atgattggaa
    11941 atacaagcaa catactatcg aaacgatcca aaacaccacc atgtccaggg ataaatttcc
    12001 cagaatcctt aacaccaaaa tgacgtttga tcgaactttc tagtaaatca ccaaattgtc
    12061 cagcaatgct aaagaaaata gcaaagactg acatcttgta aattccatat ggaagagcaa
    12121 ctgtactgtc aactatcata aagataatgg ttactaaaat tgctcctaaa ataccaccca
    12181 aggcaccctc aagggtttta ttaggcgata cccttggtgc taactttcgt ttcccatagt
    12241 tcatcccaac aagataggca ccactgtctg tcgcccagac gatacacaag gctaagagag
    12301 ccttgtccaa acctgcaaca cgagcatcta gtaaagcatt aaatccaaag cccacgtaga
    12361 agctcatagc aagagggaaa accgcatcct caatcgtata agacttgcta aaaacggtcg
    12421 ttcctaacat aattgaaatc aaaacactat aggcaaccac attcccatca actggcaaaa
    12481 aagtcaggta attttccagg ggaatggtca atacaaaggt tgcaaagagg gtcaagaggc
    12541 cctccatcgt catggtctct agacctctca tcttcaaaag ttcatgcatg gttagcatgg
    12601 ctatgattcc gattgctatc tgaagcaaga ggcccccaat cattaaaatt ggtaggaaaa
    12661 tagccagggc aatccctgca aacaaggttc ttttctgtaa atcctgggtc atatttcctc
    12721 ctaaactcct ccaaatcggc gatggcgacg attataggca agaatagctt cctgcaaggc
    12781 cgcttcgtca aaatcaggcc ataaggtgtc cgtaaaataa agctcactat aggctccctg
    12841 ccatggaagg aaattgctca aacgcaattc tccactagta cggataatca agtctgggtc
    12901 tcgtaagtcc ttaggcaaat gctgggtaaa gagatagtta ccaatcaatt cctctgtgat
    12961 gtcacctggg ttgattttgg catctaaaac atcctgggaa atcaacttaa gcgcctgtgt
    13021 aatctcagca cgtccaccat agttaagagc aaaattaaga atcaatcctg tgttgttctt
    13081 agtcaattcc tcagccttgg ttaaagcttc gaaggtttgc ttaggcaggc ggtctgtctc
    13141 cccaatcatt tgaatcttaa cattattcgc atgtagttcc gggacataat tatcataaaa
    13201 ctctactggc aagttcatga taaacttgac ttcctgatct ggacgggtcc agttttccgt
    13261 agaaaaagca tagaccgtaa taaccttgac gcccagtttg ttggctgcct tggtcacggt
    13321 ttgcaatgct tccatgcccg ccttatgtcc aaaaactcgc ggttgcatac gttttttagc
    13381 ccaacggcca ttgccatcca tgatgatgcc gatatgagca ggaacctgtg tcggaacctc
    13441 tacttccaca gccttatctt tcttaaaaaa tccaaacatg atcttattcc tattcaaaaa
    13501 tctatcgttt cattatacca tatttcccca ttttcttcta tcactaagct atttattctc
    13561 aggcaccaag cccatttttc aaaaaataag ccgcctgatt gggcgacttt atttttatag
    13621 ggagattatt atgaaaaagt tttaggagtt taagttaagg tcttcttaac ttatgaactt
    13681 agtgtacact ccctagctta aagtttcctt aagtattttt aaaaatcaaa tttttccatt
    13741 tctcctgcca atttttcttg gataaacgtg tttgatagag ttccattcgg tcttcatttt
    13801 ctaagaaatg aggagttgga cgaacttgaa aattcaaaat atcctccaaa ccataaggtg
    13861 catagagttc aaaatctgat tcttcattca agcgcagtcc aactgccgta caccgttctg
    13921 gatacttact catagcatca cgagaactgg tataggaagc agtgtgagga ctgtgctgat
    13981 gcatatagac ctgatttttc aattcccact ggtactgagg aaaatcctct ctcagctttt
    14041 tctccagtaa taaggtttcc tcataagaaa aatctggatc aaagaaaatc acatctatat
    14101 ctgtttcatg atcaaaaggg gatttgtctg acaaaagatt ccagatgaaa tttctgacag
    14161 aacctgctgc caaccacgag tctttcagac caaggtctcg gatgatcgtc agaatggcca
    14221 tcatatctgg attttctcta aaagcctcta agatttcttg cttatttttc actgtattca
    14281 taacctaagt gctcatatgc cttagcagtc gccacccgtc cagaccgtgt ccgcatgata
    14341 aaaccttttt gaatcaagta aggctcatac atatcttcaa ctgtctcacg ctcttcggcg
    14401 atattcacag aaagagttcc tagaccaaca ggtcctccac tgtacatctc aatcatggta
    14461 cgaaggattt tttgatccac atagtccaaa ccttcatggt caacatccag catagtcaaa
    14521 gccttatcgg taataatatc atcgattacc ccattcccca ttatctgggc aaaatcgcgc
    14581 acgcgcttga ggagacgatt ggcaatacga ggggttccac gactacgtag ggccaactca
    14641 gatgctgcct catgagtgat ttccatctca aaaatatctg ccgtccgctc gacaatttct
    14701 gtcaagtcag catgggcata atactccata tggcctgtaa tcccaaaacg tgcccgtagc
    14761 ggattggaga gcataccagc ccgagtcgtc gcaccaatca aggtaaaagg tggtaactcc
    14821 aaatgaacac tacgactacc ctcaccagcc ccaatcataa tatctatgta gaagtcctcc
    14881 atagcactat aaagcacctc ttccactgac attggcaaac gatggatctc atcaataaaa
    14941 agtacatccc caggctctaa ctcattcaaa atagctacca gatctccggc tttttcaatg
    15001 actggacccg acgtctgctt aagattgact cccagttcgt tggcaataac aaaggccatg
    15061 gtcgttttcc cgagacctgg aggcccaaat aagagcacat gatccagcgc ttcatcccgc
    15121 attttggcag cttcaataaa gatttgtagc tggtccttga ccttatcctg tccgatatat
    15181 tcacgtaaat actgaggacg gagcgtgcgt tctactaact cctcatcccc cattatctca
    15241 ttatctaaaa ttctactcat ggctctatta tatcaaaaaa ccaagccaca aacaaaaaag
    15301 ccacctgatt gggtgactcc taagtttagc acttatgtgg tataatattc tacgacactg
    15361 ctacaccgcc tacgaaagga ggtgagatag cccatgatgg aattagtact caaaactatc
    15421 atcggaccaa ttgtggtcgg tgtcgttctt cgtatagtcg ataaatggct aaacaaggac
    15481 aaatagtgtc aaaaaagacc tcaagcttat ttggtcgtga gcttggggtc ttttctagcc
    15541 tatgatatag aactagtact caattccttt ttattatccc atagttcacg aattttgtca
    15601 aaggtttaca ttttcttcaa ccgctgtacg acaagacggt taagattaag agaacgttag
    15661 ggattctatc aatttcatag aaattttgat ttcgtaaacg aagagacaat cttacatgtc
    15721 acttctcatt taatacgcca ctactagaca agcaaaatca ttattacagt agttccagtt
    15781 cttcaattaa cagtcactta caatcaaatt gagtttgaac tagctgaagc gaccacagac
    15841 ctatttctta gttatattcg ctaaaaaagt ccccgccaat tccccgacca aaatccgaaa
    15901 aataccgaaa aatatcgaaa aattattttt agaatagtcc ccaaaagcct gaaatagagc
    15961 taaaaaactc cacctgattg ggtggagtta agggagatta ttatgaaaaa gaaaagttta
    16021 ggattttatt aaataaagtt aggaggtctt tatttaatga ctatataata ctagaccatc
    16081 cttaaatttt gcttaagaaa aaacaagttt tgttattttt tacgattcat ccaagtcatc
    16141 cctatacaat tataggtgga taaaatttca aagcccatag aacgatagaa gcccaaggtt
    16201 ctttctgtct cttctgtcac cagctggact tgataggcat ctttgtaatc ctctaaagcc
    16261 tctttcatca aggcactacc aattccttga cgctgataga ttggtaaaac gattaaatcc
    16321 tgaaccaata ctgatgagaa tccatctcca accaaacgaa tcaagcccac cacagcatca
    16381 ccatcaagtg ccagataaat tactaatgaa tgagataagg cctgctccag catctctggt
    16441 tgatgggtat aatttgtcca accgacagcc tgatagagat gtaaaacatc ctctagcttg
    16501 acaatttctt gctttttaat agtaatcatc tcaacacctc ttaaagttct ctcaagctct
    16561 tgtactgctg tccattttta tcaaaatttt caggacgcaa ccatgtttcc aaacgatctt
    16621 tgacttgagg ccagtcctta tcaatcatag acaaccaatc cgtatctctc gtacgtccct
    16681 tataaaccac tgcctgacgg aaggttcctt cataaataaa gctcaaacgc tccgcagctc
    16741 gtctggatgg cagatttaag gcatcgcatt tccactcgta gcgacgatag tttagctcct
    16801 caaaaacata gcgagctagg agatactgag cttcagttcc tatccgtgta ccccttagct
    16861 ctggagaaaa agtgactgct ccgacttcta ttactcggtt attctggtca atacgcatga
    16921 gagaaaaagt tcccaaagcc ttaccagttg ccttgtctat gattgcatag taaaaacggt
    16981 ccttacgagc caacatctga tttaaaaggc taaccagctc ctccatatct gccactggtt
    17041 cctgaaagag gtaggtccac atttcccgag gcgtatcagg gccataaaca gctaataaat
    17101 cctccgcatg cttttccact gaaagagctt ctattcgagc ataacgccct tctaagaaat
    17161 caatagaagg cagtgcacca ggtgtataac cttccattga ctcaccaatc atctgaccat
    17221 attcatttac tggcataact ttccccttgt ttcgcttttc aaattttata gttgaccata
    17281 gtataacata ctttcataag aagattcaaa aaatccccca gattctactc tgaaggattc
    17341 ctatcttatt tcacaacaat attaaccaat ttagttggct tcaatagtcc agtggactat
    17401 tgaaggtggc agatagagct aataaactcg ttatttaacg acgatattaa cgagtttgtt
    17461 aggtaccgca atcactttca cgatttcctt accgtcaatt tccgctttga ctttttcatc
    17521 agcgagagca atttcttgta attcttcgcg tgataggtct ttagctacca tgagtttggc
    17581 acgaactttt cctttgattt ggacaacgat ttcgatttcg tcttcaacca atttactttc
    17641 gtcccatgtt ggccaagcca cgtaagagat tgagtcacct gttgctgcaa cagtttgcca
    17701 gagttcttct gccaagtgag gtgcaaatgg ggcgatcatt tggataaagc ctttagcgta
    17761 gtctacataa agtttttctt ccttgttagc cgcgttgaca aagaccataa gttgggcaat
    17821 ggcagtgttg aatttgaggg attcgatttg ctcagtaaca gctttaaccg tttcattata
    17881 aaccttgtcg agagcgccat tgttttctgc aacaatttcc ttgcttgtaa tcaaacggta
    17941 aacacggtca aggaatttac ggcttccttc cagaccttct tctgaccaag caatcgaagt
    18001 atcaagtggt cccatgaaca tttcatagac acgaagggta tcagcaccgt attgttccac
    18061 cacatcgtct gggttaacca cgttcttgag ggatttagac atcttggctg gcgcttgttc
    18121 caattcttct cctgtttcca catggaagaa agaaccatca cgtttttcaa ccttatcagt
    18181 tgccacaaga gcaccacggt ggtcacggta gctggttccc aaaatcattc cttggttaaa
    18241 gagtttttgg aaaggttctt tagtcggaac aacaccgagg tcatagagga atttgtgcca
    18301 gaaacgagcg taaagcaagt gaagaacagc atgctctgca ccacccacgt agatatctac
    18361 tggcaaccat tgtttgagga ggtcctcatc agccaatttc tcagtattgt gtggatcaat
    18421 atagcggagg tagtaccagc ttgaaccagc ccattgtggc atagtgttgg tttcacgacg
    18481 acctttgaca ccatcttcac gagtcacttc aagccaatct gtcaagttag ctagtggact
    18541 ttcaccagta cctgaaggac ggatatcctt ggttacaggc aagacaagcg gcaattcagt
    18601 ttcaggaaca gctgttgaag ttccatcttc ccaatgaatg attggaattg gttcacccca
    18661 gtaacgttga cggctaaaga gccagtcgcg gagacggtag gtaaccttct cctgaccaca
    18721 gcctttttct tccaagtaag ccacaatctt ggcaatagcg tcttctttgt tgagtccatc
    18781 taggaagtct gaattgacat gcaggccatc ctctgtgtag gcagcttctt tgacatctcc
    18841 accttcaagt acttcgacga ttggaaggtc aaattgtttg gcaaattccc agtcacgttg
    18901 gtcgtgggca ggcacagcca taaccgcacc tgttccataa ctagcaagaa catagtccgc
    18961 aatccagatt ggcatttcct taccattgac agggttgatg gcataagcac cagtccaaac
    19021 ccctgttttt tctttagcaa ggtctgtacg agccaagtca gacttaaggc tggcttggtg
    19081 tttatagtct gctacagctt ctgcttgctc tggacttgtg atagtgtcta ctaattcatg
    19141 ttcaggagcc aagacagtga aagtcgcacc gaaaagtgtg tccggacgag tagtgaagac
    19201 tgtaaattcc ttgtctgttc cttttacttt gaaagttaca ttggcaccag ttgatttacc
    19261 aatccagttg cgttgcatat ccttgataga ctctgaccaa tctagttcat ctaagtcatt
    19321 gagcaagcgc tctgcgtaag ccgtgatttt gagcatccat tggcgcattg gtttgcggac
    19381 aactggatag cctccacgct cagaagttcc gtcaggaagc acttcttcat tggcaatggc
    19441 agttcccaat tcctcaaccc agtttactgg cacttcagct tcataggcca agcctttttc
    19501 gtaaagcttg gtgaaaatcc attgagtcca cttgtagtag tttggatctg ttgtgttgac
    19561 ttcacgatcc cagtcataag aaaatccaag cgcattgatt tgacgtttga agttggcaat
    19621 gttttccgat gtaaattctg ctgggtcatt accagtatcc atagcgtatt gctctgcagg
    19681 caaaccaaaa gcatcccaac ccattgggtg aaggacattg tagccttgcg cacgtttgta
    19741 acgactgagg atatcggttg cagtataacc ttctgggtgt cctacgtgca gaccagctcc
    19801 agacggataa gggaacatat caagcgcata aaacttaggt tttgatgcat ctgttcctgt
    19861 cttaaatgta tgatgttctg cccagtaacc ctgccactta ggctcaattt ctttatgatt
    19921 gtaaaaactc atggtctctc tccaattttg tgatgttact attataccat ttttgaggga
    19981 atttgaaatg tgtttaaaga aaaaactgta tactgtatct cattaatttt gatgactatg
    20041 aattgctcta tctcccactt tttatcccat tgttatgcag taaatcaagg agtaagtcaa
    20101 gaagcttatc tcgtatagca caaaatactt actttagacc gaagataaaa ctggaatgct
    20161 aattaatgta ttcacaataa atagccgaca acctatgtcg ctttttgatt tatgaagaac
    20221 gtatagtgga ctgaatctga aatagtacga aacaattgct aaaacactta tagaaattaa
    20281 ttttactttc ccaatcgatt tgttctcatc ttatttcaat ctgctataga attcaatacg
    20341 aggatcaata aaagactacc atttggaaaa cagtagtcct tattgtttat tttgaattta
    20401 cataggcatc tacagcgata atagcttgag caacatctga aggcgaaatc ttaaacggca
    20461 tctgatgaat tgtctcaccc tccatagttg cttgtttacc aacttttatt aaatcatcat
    20521 atccaacttg atccaaatgc atttctttta gagttgttgg cataccaatt tttttgtaaa
    20581 actcaatata cttatcaagt tcttctttag gtctattttc caataatagt tgtactaaag
    20641 ttccataagc tactttttca ccatgtgtta aatgatgaat gtcacctgtc aatgcagtaa
    20701 aaccattatg aattgcatgc gccgcagcta atcctccact ttcaaaacct aaaccactca
    20761 ataaagtatt agcttcaaca atgttttcta atgctggtgt caccacttta gcttcacaag
    20821 ctgccatagc ctgtaaacca tctgcaaaca gcgtttcttc acatttcttc gcaattgcaa
    20881 ctccagccaa tgtttgctgt tgtcccaaca tagtttttcc atttgcctgc ataaccgcac
    20941 gcgcctcaac ccaagttgct aaaccatctg caataccaga cgctaataaa cgcttagggg
    21001 cttgtgaaat aacttttgta tcaaccaaaa ctaaatctgg atttttagaa taaaatagat
    21061 aatgatcaaa tgcaccttca tctgtataaa taacagataa agcagataca ggtgcgtcgg
    21121 tcgatgcaat tgttggagca ataataacag gcttttcaat caaatctgca atagcttttg
    21181 cgctatcaat cgtctttcct ccaccaagac cgataatact atcacaattt tctttctcag
    21241 ccaaggcaac aactcgattg atttcattgt cagaagcttc accattaaat agcgccagaa
    21301 caatatggaa accataccta tgtaggtaat cttcaaatcg ttttccaaca atatcataaa
    21361 ccaactgatc gcataataga atagggcaat ttcccaaatc caaaattgat ttggcatttt
    21421 caaacaaggc attttccccc tgaatatatc tagaaggact agcaaaaatt ctcatatgta
    21481 tttcctctct atctaaatgg aacgactatt ttgagtaaca aaccaatcgt cagaaaaatc
    21541 atcaaccgcc ttttggatag atggcatggc gaaagctgat tcaaaaacat ctgctcctgc
    21601 tgtaacagca tgcgcacctg cagctaaagc attatttact tgtgctacat ttttaaagga
    21661 tgcagctaaa atcttactag gagagttctg tctatcaata gcaagagcta attgacgaat
    21721 gacagaattt gaatcaatgt tcagattttc cattctatta taatatggag ctaggtaatc
    21781 cgctcctgct tcgatagcta ataatccctg aataactgta taaatagctg ttgcagtgat
    21841 atggtagccc tcttttttta gcacctttat tgcacgtaat ccagctggag taacaggtac
    21901 tttgataaat atatcatctc ctgcttgtct tcgaatttca cgagcatcct ttaagatgcc
    21961 ttcaaaatct tgagaaatca cctgaacatg aatagagggt gtggagccaa tcaattctct
    22021 tacatctttg attcgttcaa aaaaattaat agaaccctct ctttttgcaa tagtgggatt
    22081 tgaagttacc ccagctagcg gcaaaatttc agaccacttt ttaatctcat ctaaatttaa
    22141 tgtgtcaagc ataaattcca taaaaagaac ctctttattt tacaaagtat gctcagtacg
    22201 tcctataatg tcatcttggg ttgccttaga aagaacattg aagaatgcag agtatcctgc
    22261 aacacgaaca attaagtctc tgtgtttttc aggatgtttc tgagcgtcaa tcagcgtctc
    22321 tctggaaaca acattgtatt gaatatggta cccatgtaaa cgattaaaga atgttcgtaa
    22381 caaagcaatt agttttaatt tatcttcttc tttggctaag gtttgaggat ttactttctg
    22441 atttaagaga accccaccta cgatttcatc tgttggtaat tttgaaacag attttaaaac
    22501 agatgtaggg ccgtgttgat ccatattatg tgatggtgaa caaccctctg ctaacggtgt
    22561 acccgcgttg cgtccatctg gagttgctaa tgttccacgt ccctgcccta cgttggctga
    22621 gatagaagat gttcctgaat aacgaattcc tccaataggc cctcttccat aacgtgtatt
    22681 aggatattta gcaatttcat caacataaat gtcataagca gcagtaacca atttgtcagc
    22741 ataatcatca tcattaccat acttaggtgc atcatgaatc aacatttctt gaatgacctt
    22801 accttcttct ccggcataat ctgtttccag tgcatgccaa agctgacttg ggcttatacg
    22861 ttcttcctca aacaccaatt ttttaattgc agctaatgaa tccgacaaat ttgcaattcc
    22921 aacttgcaat cctgatatat aatcatatac tgctccacct tctttaaggt gttttccacg
    22981 accaatacaa tcatcagtca atgctgaaca tagaatatca ggaacttctc gttccaatga
    23041 taaatcaata gaattttcaa caataacact cattcgtgtc aaatatctta gtgttttatc
    23101 ccaagcattt tctaattcag aaaagttctt catatcctta aaatgaccaa agcttggtgc
    23161 aaaccgttta cccgaagccg gatcaattcc atcattcatc gtgataagta gaaccttagg
    23221 gaagttcata taactcatac ctgtgcaacg atagccccat ttccctggaa ctgccgtttc
    23281 aacacatcca atggcactgt aatcataagc atcatcttcc aatactcctt ttgcaataaa
    23341 agaaggaata ataatctcat cattattaaa tgcaggcata ccaaaaccaa gtttcatcac
    23401 ttcaatacac tcattcatga aacgagcatc taaacctgca tggtaacgta cagttagatt
    23461 aggttgcggt agatgggttt gtgcgactga ttttaatacc aaataagata atgggttaac
    23521 agcatccttc ttatctcgag tctgtccacc aattgtaaca ttttgatata aaggacttcc
    23581 tgctgaagaa aatgtatgtg attgactgcg aaccttattg attgtaattg tcttaatcca
    23641 aagatttgtc agacgttcaa caatgctatc ttctgtttct ttaccacttt ctaaatcagc
    23701 cttcatatat ggatacatat attgatcaaa acggccatat gaaagagagt ggccattaga
    23761 ttcaatttgt aaaatacatt gaataaacca aactgattga atagcttctg caaaagtagt
    23821 tgccggttca tatgggactc tagagcaaat atctgcaatc tcaagtaatt ctttcttacg
    23881 tttaggattt gcattttcgg ctaaactttt agcaagagca acaaagcgct ttgcatatac
    23941 tttaatagca tcgattacga taaatataga gtcgtaaaaa tgatatttat caatacttgc
    24001 tggatctgtt aaatctagag ctacttttgc tttacgagcc cgctcttcaa aacctcttaa
    24061 accaaattgc aaaagtttct gatagttaac tgctaagtga gcatctccag aattcatctt
    24121 accttccata ccgaagaatc ctgtttccat ataaacagac acttcttcag gtaataaggc
    24181 accagctcta gcacgtaaat tattattttc ccaaaacgga gcaatacttc taagttgttc
    24241 ttttgtttct tctgtaatat agaaaacatc tccatcacgc ttttcaaaaa gatccaactc
    24301 attgagaaca aattctagcg tatattccgg aaaaatagga gcatctttat tggaagaagc
    24361 ttgatttccc gcaatcatag attcttcttc aatatagata gacatatttt ccaaaatttc
    24421 tttcagcata tatgcacgtt ttaggacatt aggttgttcc ttgtatcgtt cataggcgcg
    24481 tgttgctaga actgctctct cagcatcaat ataaggtttt ttatttaaaa catcttctcg
    24541 atatttgttc atcctttcag taaggctgcc aaaatattct gtcttgattg ttgttcttgc
    24601 tacttctgta tttaccatcg taagcacctt tctttcgaaa atatttttat ttctttcgaa
    24661 aatataatac cgatttttat agaaattttc aagcttttaa ctagaaaaat tttaactctc
    24721 taatttttag cacaaatgtg tgagtttaac agtaacacac aaatacctct aagattgttt
    24781 gatttcattt tggtaagcta aacgatcttg tatcttaaag aatggaaagt aaaccactgt
    24841 agatatcacc aatactaata actgagtcac aacaccttgc catccaccca ccaaaaatcc
    24901 tgatagaata gctggtgtac tccaaggcaa tgttacccct gagaatggct gcatgaaacc
    24961 tgttgcaata gttccatata ctatcacagc tgcaagtaca ggaacaagaa tgaaaggtac
    25021 aaacataact ggattcatga caatcggaaa tccaaataca actggctcat ttacgttaaa
    25081 tattgctgga aaagctgcaa cttttcctaa ggcttggtat tgttttgatt ttgctgcaaa
    25141 aagcatggca actacaagac caaacgtaat ccctgaacct gatagaatta aaaatgaatc
    25201 taaaaattgt tgagtaacaa tatgtgcacc attttctaat gatagattac cagaggctaa
    25261 catagcttta ttagcatcaa gattagataa aagcagagct gtcactactc catttactac
    25321 cgattgtcca tgaacaccaa accaccacaa aaatgatata aagaatgcaa ttccaattgc
    25381 accatacaaa gatccagtta aaccttgcaa tggaacttga atagcagaat aaatcatttc
    25441 tatgaatgtt ccgccattag tcaatgactt cgctaaaata tatacaatca tagaaagtaa
    25501 gaaaattaca aatgctggaa tcattgcttc aaactgtttg gcaatagctt gtggaacttg
    25561 ttctggcatc ttaataacaa tttttctctt tataaagaag gtataaatac ttcctactac
    25621 caaacctata atgatagcac cgataattcc ttgacctcca aaccaaactt tactaatagc
    25681 atccccaatt gcctcacctt gtttagggat ataagatgat cttagcaaaa taaagaatgc
    25741 agatacagat agaactccag ctggtaaagc ctctactccg ctattcttag cataagaata
    25801 ggcaattgaa aaacaagaaa ttagacccat aatagcaaaa gttcctgaat atacttgcat
    25861 aaacggctct gtccaattag ctccaaaaac actagcaatg ctcttattta atccttcgaa
    25921 cggcaattgt cccataatca agaataaact accaactact gtcaatggca aaattgctaa
    25981 catcccatct tttagagcta taatgccacg catattcaca aacttcatca tcggtgcaat
    26041 gattttctga acatccatct ttgccataat aaatctcctt ttcttaccca ctaatcaaag
    26101 atagggccaa atctaatact tttttcccat ctaacatacc atagtccatc atcggaataa
    26161 cagctatcgg aacatcacac ttatcacaaa tttcttttga tttatctaat gtataagcaa
    26221 cttgtggacc caatagtgca acatctatat ttggcgcata atccgctaat ttagactgag
    26281 aaaacgcctc tatttctgcc tcaactccac tagcttgcgc tgcaattttc atattattta
    26341 caagcatacc agtagaaaaa cctgctgcac aaaacaaacc aatcttcacc attatgtttt
    26401 cctcctctat gttaataaca atgataatac tctagtatta ttttttatga agttcttttc
    26461 tcaaactaat aatttctttg attaaattaa tctccgtcat actagtcatg agatgatctt
    26521 gtgaatgaac aaagagagct gtaatttctg tctttgtacc agacgcttct tgtgccaaga
    26581 attttgtttg tagattatgg gcttctaata aggcggcatc agctaactgt atttcttctt
    26641 cacaccgttc acatgtacca ttttttatgt aatctaacgc tttataaata tgttgtttcg
    26701 catcaccagc atataaaatt aaacccatga taatttgatc tggaacaata atttccataa
    26761 attcc
//
LOCUS       DAVGJJ010000020        26408 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000020 DAVGJJ010000000
VERSION     DAVGJJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.020"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..809
                     /locus_tag="VVE35_000764"
     CDS             <1..809
                     /locus_tag="VVE35_000764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3060997.1"
                     /translation="DEFIDLMISMIYGAQSLNRKIEGNSKIVLLLRSDILKLLFGKSG
                     DVNKPITSGGIELIWYESNAAASDSPLIKMVLYKMSKSIDDNLPLSEIRRNFFPTKDT
                     IFKYMMSRSFGRPRDIVSFFNIYKKKFPEDQKIVLNNMQRVEKEYSEWFYKEISNELK
                     ITGKFDEVDKLLKSISELGKYEFTYHKLFDFMQKRQGNTDELLELLTLLVQLGVLGIR
                     LNTGNIEFRYRDYNIDKPVNIHTKFVVHYGLRKLLSISFNDDNEKNIIYQ"
     gene            845..931
                     /locus_tag="VVE35_000765"
                     /pseudo
     CDS             845..931
                     /locus_tag="VVE35_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
     gene            1053..2315
                     /gene="purD"
                     /locus_tag="VVE35_000766"
     CDS             1053..2315
                     /gene="purD"
                     /locus_tag="VVE35_000766"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU3060998.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            2718..3206
                     /gene="purE"
                     /locus_tag="VVE35_000767"
     CDS             2718..3206
                     /gene="purE"
                     /locus_tag="VVE35_000767"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU3060999.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDRSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            3193..4284
                     /gene="purK"
                     /locus_tag="VVE35_000768"
     CDS             3193..4284
                     /gene="purK"
                     /locus_tag="VVE35_000768"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU3061000.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            4294..4521
                     /locus_tag="VVE35_000769"
     CDS             4294..4521
                     /locus_tag="VVE35_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU3061001.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            4584..5882
                     /gene="purB"
                     /locus_tag="VVE35_000770"
     CDS             4584..5882
                     /gene="purB"
                     /locus_tag="VVE35_000770"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU3061002.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(5937..9875)
                     /gene="strH"
                     /locus_tag="VVE35_000771"
     CDS             complement(5937..9875)
                     /gene="strH"
                     /locus_tag="VVE35_000771"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU3061003.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYDSDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYTNTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGTPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPSTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQNPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(10191..10907)
                     /locus_tag="VVE35_000772"
     CDS             complement(10191..10907)
                     /locus_tag="VVE35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3061004.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            11254..13041
                     /locus_tag="VVE35_000773"
     CDS             11254..13041
                     /locus_tag="VVE35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU3061005.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            13038..13514
                     /locus_tag="VVE35_000774"
     CDS             13038..13514
                     /locus_tag="VVE35_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU3061006.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            13542..14447
                     /locus_tag="VVE35_000775"
     CDS             13542..14447
                     /locus_tag="VVE35_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3061007.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            14434..15249
                     /locus_tag="VVE35_000776"
     CDS             14434..15249
                     /locus_tag="VVE35_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3061008.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            15256..15660
                     /locus_tag="VVE35_000777"
     CDS             15256..15660
                     /locus_tag="VVE35_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3061009.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            15954..17120
                     /locus_tag="VVE35_000778"
     CDS             15954..17120
                     /locus_tag="VVE35_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU3061010.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            17236..18273
                     /locus_tag="VVE35_000779"
     CDS             17236..18273
                     /locus_tag="VVE35_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU3061011.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLEHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            18479..19333
                     /locus_tag="VVE35_000780"
     CDS             18479..19333
                     /locus_tag="VVE35_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3061012.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            19483..19740
                     /locus_tag="VVE35_000781"
     CDS             19483..19740
                     /locus_tag="VVE35_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEU3061013.1"
                     /translation="MTNSKYITCLKRSEGQLCRIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(19782..20099)
                     /locus_tag="VVE35_000782"
     CDS             complement(19782..20099)
                     /locus_tag="VVE35_000782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061014.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(20153..20436)
                     /locus_tag="VVE35_000783"
                     /pseudo
     CDS             complement(20153..20436)
                     /locus_tag="VVE35_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(20426..20587)
                     /locus_tag="VVE35_000784"
     CDS             complement(20426..20587)
                     /locus_tag="VVE35_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU3061015.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            20657..21421
                     /locus_tag="VVE35_000785"
     CDS             20657..21421
                     /locus_tag="VVE35_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU3061016.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKAYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            21986..23365
                     /locus_tag="VVE35_000786"
     CDS             21986..23365
                     /locus_tag="VVE35_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU3061017.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            23379..24044
                     /locus_tag="VVE35_000787"
     CDS             23379..24044
                     /locus_tag="VVE35_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU3061018.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            24174..24254
                     /locus_tag="VVE35_000788"
                     /pseudo
     CDS             24174..24254
                     /locus_tag="VVE35_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            24400..24939
                     /locus_tag="VVE35_000789"
     CDS             24400..24939
                     /locus_tag="VVE35_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3061019.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVEILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGVVNCGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEMDVFVTNFVYEKEGQSRKKSMSYESVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            25006..25405
                     /locus_tag="VVE35_000790"
                     /pseudo
     CDS             25006..25405
                     /locus_tag="VVE35_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
     gene            25521..>26408
                     /gene="pavB"
                     /locus_tag="VVE35_000791"
     CDS             25521..>26408
                     /gene="pavB"
                     /locus_tag="VVE35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU3061020.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT         7314 a         5054 c         6011 g         8029 t
ORIGIN      
        1 tagatgaatt tatagattta atgatttcta tgatttatgg agcccaatca ctcaacagaa
       61 agattgaagg aaattcaaaa attgtattgc ttttaagaag tgacattctt aagctattat
      121 ttggaaaatc aggcgatgtc aataagccga taacatctgg gggaatagag ttgatttggt
      181 atgaatctaa tgccgctgcc tcagatagcc ctctaattaa gatggttttg tataaaatga
      241 gtaaaagtat tgatgataat cttcctttga gtgagatacg aaggaatttc tttcctacaa
      301 aagatacaat atttaaatat atgatgtcta gaagttttgg tcgacctagg gatatagtat
      361 cgttctttaa catatacaag aaaaaatttc ctgaggatca aaaaattgtt cttaataata
      421 tgcaacgagt tgaaaaagaa tattcagagt ggttttacaa ggaaatatct aatgaattga
      481 agataacagg taaatttgat gaagttgata agctattaaa atcaatttct gaacttggta
      541 aatatgagtt tacctatcat aagttattcg attttatgca aaaacgtcaa ggtaatactg
      601 acgaattatt ggaattgtta actttacttg tacaattagg agtattgggt attaggttga
      661 atacaggaaa tattgaattt agatatcgag attataatat tgacaagccg gtaaatatcc
      721 atacaaaatt tgttgtccac tatggattga gaaagttact gtctattagt tttaatgacg
      781 ataacgagaa aaacattata tatcagtaag attaaaagaa tgttgaaggt aaagtattaa
      841 acgtaatcat cgtgaccaag aatctatcga agcggcagac aaatatggct tgaccatggt
      901 ctttacaggc gtgagacatt ttagacatta agaaggtaaa gggtagaaaa aaatttcttt
      961 cctttttttg cgtaaaaacg cggagtgaaa caagattaaa acgaacgttt atgttataat
     1021 gttagtaaat gattcgtaaa agaggttgag aaatgaaact gcttgttgtc ggttcgggtg
     1081 gtcgtgagca tgcgattgct aaaaagttac ttgaatcaaa agacgttgaa aaagtctttg
     1141 tagctcctgg gaatgacggt atgactctgg atggtctgga attggtaaat atctctattt
     1201 ccgaacatta taaattgatt gacttcgcaa aggccaacga tattgcttgg acctttatcg
     1261 gtccagatga cgcccttgcg gctggtatcg tggatgattt ccatgcagct ggtctcaaag
     1321 cctttggtcc gacaagattg gcagcggagc tggagtggtc caaggatttc gccaaggaaa
     1381 tcatggtcaa atatggtgtt tcgacagcag cctatggcac attttcagat ttcgaggaag
     1441 caaaggccta tatcgaaaag catggtgcgc ctatcgtagt caaggcggat ggcttggcgc
     1501 ttgggaaggg tgtcgtcgtt gctgagacgg ttgagcaagc ggtcgaagcc gctcatgaga
     1561 tgcttttgga caataaattt ggtgactcag gtgcgcgcgt ggttattgag gaattccttg
     1621 aaggagagga attttcactc tttgcctttg tcaatggtga taagttctac atcatgccaa
     1681 cggctcagga ccacaaacgt gcctacgatg gcgacaaagg gcctaacacg ggtggtatgg
     1741 gtgcctatgc gccagttcca cacttaccac agagtgtagt tgatacagcg gttgatacca
     1801 ttgtcaagcc agttctagaa gggatgatta aagaaggtcg cccttatctg ggagttcttt
     1861 acgcagggct tatcctgaca gctgatggac cgaaagtcat tgagttcaac gctcggttcg
     1921 gagatccaga aactcagatt atcttgcctc gcttgacctc tgactttgct caaaatatca
     1981 cagatatcct ggatagcaag gagccaaata tcatgtggac ggacaagggt gtgactctgg
     2041 gtgtggttgt cgcatccaag ggctacccgc tagactatga aaggggcgtt gagttgccag
     2101 ccaagacaga aggcgatgtc atcacctact atgcaggggc taagtttgcg gaaaatagca
     2161 gagcactgct ctcaaacggc ggacgagttt atatgctcgt taccacagca gataccgtca
     2221 aagaagccca agccagcatc taccaagaac tataccaaca aaaaatagaa ggactcttct
     2281 accgaacaga tatcggaagc aaggcaatta agtaaagata taagaataac gcgccgtagt
     2341 cgccaaacac gataatggtc gtcgtggtga aaagaccaga acagtgaatg ttctggtcag
     2401 ggggaaactt ggagacctta ggctcaaagt ttaggaatga aaccgaaggt ttgcttccgc
     2461 ctccatcacc taagaccatt atcaaaaaga aaaataaaaa ttcacaaaat acgttaatga
     2521 tcgtatggtt tgcgagcgtt agcgagctaa tatagaacaa tcaccgccgt tgtgaaagaa
     2581 cgattggatg ataatccaat cgttcaggga aattggaaga ccttgggttt ccaatttagg
     2641 catgagacac ctttggtggc tgctgccgtc cctcacaagc taaggtgatt gttgaaaaag
     2701 aggaaaaagg agaagaaatg aaaccagtaa tttccatcat catgggctca aaatccgact
     2761 gggcaactat gcaaaaaaca gcagaagtcc tagaccgctt cggtgtagcc tacgaaaaga
     2821 aagttgtttc cgcacaccgt acaccagacc tcatgttcaa acatgcagaa gaagcccgta
     2881 gtcgtggcat caagatcatc atcgcaggtg ctggtggcgc agcgcatttg ccaggcatgg
     2941 tagctgccaa aacaaccctt ccagtcattg gtgtgccagt caagtctcgt gctcttagtg
     3001 gagtggattc actctattct atcgttcaga tgccgggtgg ggtgcctgtt gcgaccatgg
     3061 ctatcggtga agctggagcg actaacgcag ctctctttgc cctccgtctc ctctctgtag
     3121 aagataggtc cattgcggat gcacttgcca actttgctga agaacaagga aaaatcgcag
     3181 aggagtcgtc aaatgagctc atctaaaaca atcggaatta tcggtggcgg tcaactgggt
     3241 cagatgatgg ccatttctgc tatctacatg ggccacaagg ttatcgcgct ggatcctgcg
     3301 gcggattgcc cggcctctcg cgtggcggaa atcattgtgg caccttataa cgatgtggac
     3361 gccctccgtc agttggcaga ccgttgcgat gtcctcactt acgagtttga aaatgtcgac
     3421 gctgacggtt tggatgccgt tatcaaggat ggacaactcc ctcaaggaac agatctgctc
     3481 cgcatttcgc aaaatcgtat ttttgaaaag gactttttgt caaacaaggc tcaagtcact
     3541 gtggcaccct acaaggtcgt gacttctagc ctagacttgg cagatatcga cttgtcgaaa
     3601 aactatgtcc tcaagactgc gactggtggc tacgatggtc atggacaaaa ggttattcgt
     3661 tcagaagcag acttggaagc agcctatgcg ctagcagact cagcagactg cgtcttggaa
     3721 gaatttgtca actttgacct tgagatttct gtcatcgtgt caggaaatgg caaggaggtg
     3781 acgtttttcc cagttcagga aaatatccac cgcaacaata tcctgtctaa gaccatcgta
     3841 ccagcccgca tttctgaaag tctagcagac aaggctaaag ctatggcagt gcgaatcgca
     3901 gaacaactca acttgtctgg aactctctgt gtggaaatgt ttgcgactga tgatgacgtt
     3961 attgtcaatg aaatcgcccc acgaccacat aactctgggc actattctat tgaagcctgt
     4021 gatttctctc agtttgacac ccatattctg ggtgttctcg gagcaccatt accagtcatc
     4081 aaactccatg cgccagccgt tatgcttaat gtcctcggtc agcatgtcga ggctgctgag
     4141 caatatgtga cagaaaattc aagcgcccac ctccacatgt atggtaaaat agaagcgaag
     4201 cacaaccgca agatgggaca tgtgaccttg tttagtgatg taccggatag tgtggatgag
     4261 cttggggaag ggattgattt ttaggacaag tctatgatac aaattatcgt taatacattt
     4321 attgaaaagg ataagactgg agcagttgtt gaagtgttgt atgccagtgc tgaccaagat
     4381 aaggtacaag ctaaatatga agaactagct gcacaatacc ccgaaaatta tttagctatc
     4441 tatgatgtac cgctggatac ggatttgaat acactagatc attacccgtc tgtgtttatt
     4501 gaaaaagagg agtttgagta gaaatcttgg tttacctaga tagcttattc ccaacagctt
     4561 aagaagaaag gaaaaattaa cacatgatca accgttactc tcgccctgag atggcgaata
     4621 tttggagtga agaaaataaa taccgtgctt ggcttgaggt ggaaatcctc tctgacgagg
     4681 catgggctga gttgggggaa atccctaagg aagatgtggc tttgattcgc aagaaggcgg
     4741 actttgacat cgaccgtatt ttggaaattg agcaggagac gcgccacgat gtggtggctt
     4801 tcacgcgtgc ggtttctgag actcttggtg aagagcgcaa gtgggttcac tatgggttaa
     4861 cttctaccga cgtggtggat actgcttatg gttacctcta caagcaggcc aacgacatca
     4921 tccgtcgtga ccttgaaaac ttcactaata tcatcgctga caaggccaag gagcacaagt
     4981 tcaccatcat gatggggcgt actcatggtg tgcacgctga gccgacaacc tttggtctta
     5041 aattggcaac ttggtacagc gaaatgaaac gcaatatcga gcgtttcgag catgcagctg
     5101 ctggtgtaga agctggtaag atttctggtg cggttgggaa ctttgccaat atcccaccat
     5161 ttgtagagga gtatgtctgc gataaacttg gcatccgtgc ccaagaaatc tctacacaag
     5221 tccttcctcg tgaccttcac gctgagtact ttgcggttct tgccagcatt gcgacttcaa
     5281 tcgaacgtat ggcgactgag attcgtggtc tacaaaaatc tgagcaacgc gaagtagaag
     5341 agttctttgc taaagggcaa aaagggtctt cagcaatgcc tcacaaacgc aacccaatcg
     5401 gttctgaaaa tatgactggt ctggcgcgtg tcattcgtgg tcacatgatt acggcttatg
     5461 aaaacgtcgc tctctggcac gaacgcgata tttctcactc atcagctgag cgtatcatca
     5521 caccagatac gaccattttg attgactaca tgctcaaccg ttttggaaat atcgtcaaga
     5581 acttgacagt cttcccagaa aatatgatcc gaaacatgaa ctcgactttt ggtcttatct
     5641 ttagccaacg ggctatgttg acattgattg aaaaaggcat gacccgtgag caagcctatg
     5701 acttggtgca accaaaaaca gcctactctt gggacaacca agtagacttt aaaccacttc
     5761 ttgaggcaga ttcagaagta acatcacgtc tcactcaaga agaaatcgat gaaatcttta
     5821 acccagttta ttacaccaaa cgagtggatg atatctttga acgtcttgga ctaggtgatt
     5881 aattaaaaaa taaacagcga gcttcaatct cgctgtttat tttttatcga aaagacttag
     5941 tcttcttttc ttttagtgag tccataggct gctagtgtgg ccatgagtcc tgcgactact
     6001 agtcctgcag aatcgtgagt tcctgtttca ggaagttttt tctctattac cacaggagct
     6061 ggattttgag gaagagcttt gctttcctca gcaggagcag ttgatggagc tggttggctt
     6121 gggatttcta gttttggttt ttcttcagca atagcggctt gtccgttttc atcgcctaca
     6181 tgtgttacca tagttccgac ttcgactatt tgagtaacgg cttcctgtgc tacgacacta
     6241 tttacaagtg ttttcacttc cttaccatcg gcagaagtgc tcacagagta gaagttgcta
     6301 cgatgtccat tgacaccctt agtaatgact tgtgtttttc ctttgagtaa gagtggattt
     6361 tcacgagtca ctgtggtaga tggaatttct tcttcttgga tatccagtct aggttttacc
     6421 tcagtagttg gtgcaagacc actttcatca cccttgtgag ttacaggagt gccaacttca
     6481 accacttggt ttataacttc tttggttacc tggctatcaa ggactgtttc tgttgttttt
     6541 ccattttcag tgagtacaga gatgtaatga gttcgttcac ctttgactcc tgctgtgata
     6601 atattttcct gaccagctgg gaggttagga ttttctttct tgataacttc aaatggaatt
     6661 tcttcagttc ttgtgatgag ttctggtctg gtttcaacat tggcagccac ttcattttca
     6721 tctaggcttc ctgaatgagt tgcagctggt ttgaggcctt gaagagcggc ttttaggttg
     6781 gctacaagtg tgtcaagctc agcttgttta ttacggttga ggttgtaatt tagagctgtt
     6841 ttagctgcgt caagggcctc aagactttct ttactatatc cttctaagtt tgtaggaatt
     6901 ttagctaatt cttcgcggag agcattataa ttagcacgga agtagtcttt gttgtggtct
     6961 gcaaaggcag tcatgagttc aaagatttcc tcttccttgt attcagcgct tggtctatct
     7021 gcccagattg aaagcatact tccgactgtt ggaagatcta cttcaggata tttggtagaa
     7081 gctagttgat tgaatggtgt ttttccagta ttctcaatag ctttcttgag gaaaccacca
     7141 ccatcttctg gtttttgacc aagaatgtag taccagtcac cgttggtatt caaaaattta
     7201 tagcctttgc ttgctaggta ttgaggtgat gcgaggttat atccccacca gcctttagac
     7261 cagtaagaaa tcaagacatc tttgtcaaac tgaacatcgt ccttgtcttc atagtagaag
     7321 ccatcgttga aggccattgg ttgaagccct ctttctttgg ccatagctgc gagggtgttg
     7381 gtatattcgg caaatttgcc atagagttga taccacttga ggtagtacca gccttgggca
     7441 ctagtcgcat cgttggcgta ttcgtcagta ccaaagttga aaatctttgt tttacctgca
     7501 aagaagtcca tgtatttacc gatgagggct tttacaaagt tcatcgcttc ttcgtttttc
     7561 aagtccatag ttgtttttga aactttatca aagtgggctt gaggattttt aatacctaat
     7621 ttttccatgg caaccagcat agcatccatg tgacctggac tgttaatagc tgggatgaga
     7681 ccgatgtcct tagatttagc gtattcaatt agctctgtca cttctgcctg tgttagtgca
     7741 gtaccgtttg gatcgtcgta gtaagcttta gttccttcga taatagcttt tttaacgtca
     7801 tcactagcat aggtttttcc gttggcagta atggtcatat cattgagtag aaagcgaagt
     7861 ccgtcatttc ctagaaggag atggacatca gaatatccga gctcactggc cttgtctacg
     7921 atgcgtttga gctggtccag agtaaagtat ttgcgtccag catcgattga gattaccttg
     7981 tttttggcaa gtttttcaac ctcacgttta gcttcttctt ctttttgagc ttcaggcgtg
     8041 agggtcaagt tgttgacagt ttcttgaagt ttagcaatgg cttgatcaat cgtatcttgt
     8101 tgggcacggc taaggttgct atcgagagag cgaatagctt tttcagcttc ttttacggcc
     8161 gcgacgcttt ctgcagtata acggttcagg tcttttggta cctcgttaag tgcttgctct
     8221 gcagattcat aatcagctgc gaagtattca gcgttggcat ttgcaaaatg acgcatgagt
     8281 ttgaagaggt gtgatggtga ataacgtgca gatggagtgt cagcccaagc agctaccata
     8341 ccaccgatga ttgggatatc agctccttct gtttttggta cagaagtgat tggtgtgttt
     8401 ttaataccat tgagcccctg atcgagattg taccagcctt ggccatcagc gtttcgtcca
     8461 agaacgtagt accaagcatc attggtatta aggatttggt gacctttttc agctagtagt
     8521 ttagaagaag cgacatcgta gcctccccaa ccaccagtcc acatagaaac gatgatgtct
     8581 ttgtcaaaac taccaaagct tgtgtcgcta ttgtagtaga taccgtcgtt aaaggccatt
     8641 ggtttgagac cgtgcgattt tacaatacga gcgaggtcat tggcgtaggc aataaatttt
     8701 tcatagcctt ttacagggta gccttcgttt ggatagtatt tatcagcttg aagcacactc
     8761 caacctttag catctgtcgc atcattggca tattcatcaa gtccgatgtt gaagatttca
     8821 gtctttttcg cgaaataagc agcatacttg tcgataaggg cttttgtaaa agcgacagct
     8881 tgttcgttgt caagatcgac agtacgggct gattccttcc caaaatagct aaagttaggg
     8941 ttttggattc ccaattcttt catggcattg agaatcgcat ccatgtgacc aggactattt
     9001 actgtcggaa tgagaccgat acctttatct ttggcatagt taatcagatc tgtcatttga
     9061 ctttctgtta agtgattgcc gtttggatcg ttgtaataat catttgtacc tttttcaatg
     9121 gcgcgtttga catcgtcact gtcataggtc ttgccgttag ctgtgatgct catatcgtcc
     9181 aacatgaaac ggagtccatc atttccgact aataggtgta aatcagtgta gccataatgt
     9241 ttcgctttat cgatgatttc cttgagctgt tctggtgaga aatatttacg tccagcatca
     9301 atagaaacaa ttttcttttt cgctagtttt tcatttacag ttgcagcacg ttcctttcct
     9361 gcctctgttt ccggtttgtc agcctctgct ttcgcttcat cttttttagc tggtttatct
     9421 ttgtcagtct tgtccgtatt tgactcttta gaatcaacct ctttcgcttc ttccttttta
     9481 gggctagctt cttctgcctt tttattagca gtttcttttt cagcagaagt tggagttacc
     9541 acttctgctt tatcactagg agttgaacta acttcctctt gtggtttttc ttctgttttt
     9601 ggaagactag ctaccttatc agtagctgga gtttctgttt ctacagtttt tggagcttct
     9661 ggttgaagca ctgctttagg tgtttcctca gtccgatttt cggatgattg aggggaatca
     9721 gaaaccgtat ggatggtcgg ttggttttct gtagtagtag gagtaactcc atcggctgca
     9781 acagtctgtg cttggaaggc aaatccaatt agaacagaag ctgctcctac agcgtattta
     9841 cgaatagaaa aacgctgttg tttttcatgt ttcattgcaa aacctcctga ttgcattgtt
     9901 atattgatag cgattatata aatcaacgcc tttattttat ttcttatatt aacgagagtc
     9961 aagaggagat gacaaaaaac tataataagt ataaaaaaat ataaaattta aacttaagat
    10021 ttcagattgg tcggaaaaaa tacgtatata tatctagtat aatttttggt tctatttcta
    10081 taaaatattc cacaaattat agaattttcc aaaaataggt aagcgctacc tttttggtgt
    10141 ggtataataa gcatagaaaa agcccgagcg attaggctca ggctttcttc ttagtgatca
    10201 cggtcacatg agatgaattt aatcttgtag taatcagatc gtttgtaagt ttcactgtat
    10261 tctaaaactt ggccagttga ttcgagtttg gtgattttag tttgtaggac agtagggaat
    10321 tgttcatcga ctccgaggac tgaagccgca tgttctggag ttggaaagac tatttcgttg
    10381 atttcttcaa agtgttcatc attcatgtga atgtggtagt ctaacttgaa acgattatag
    10441 atagaactat agtattcaag gtttggataa tttgcgttga tatattgttc tgggatgtag
    10501 gatgtatggt agatataaac gacaccgttt gattcgcgga tacgttcaat cttgtagtag
    10561 aattgatcgc cgcgtagacc caatttttcc aagtaaacaa gcttgtttcc gcgttcaatt
    10621 gaaagaacag ttaccttatc atctttagca ttgaagagct caatatctga aaactctaca
    10681 agcttgtgtt tgcgtgcacg tgaaacgaag gttccttttc cttgttggcg gacaatatag
    10741 ccatctttgg caaggtcgtt taaggcgcga acaactgtga tagagctgac atcgtacatt
    10801 gaaatgagtt ctgcttcagt gtaaaattta tctccactgc taaactgccc agagatgatt
    10861 ttatttttta attcgtcttt tatgtattga tatttgggaa ttgccatatt tttacctcta
    10921 ttttccttct ccatagaaaa ttttaccata aaagcgaaaa aaatagtagt cttttgaata
    10981 aaaaattaga ataatagttt gaaaaattaa gcgatatagg gattgctcat attttatcaa
    11041 tgtattctat acaaaattat tttaggtcct atattccgaa tttaaagagc gtttgaaatg
    11101 ggtctaaaaa tgtttgaaac taattgaatg tgtgtataat agtaaaaatg cagtaaatat
    11161 cccgacattt ctattgacaa ttcaaacaga ttggtttata attaatataa caacaaatga
    11221 aagcgcaaac tttcgcggtc ggaaggtagt tttatgacac gatttgagat acgagatgat
    11281 ttctatctcg atggaaaatc atttaagatt ttatctggtg ccattcatta ttttagggtt
    11341 cctccagagg attggtatca ttcgctctat aacttgaagg ctcttggttt taatacggta
    11401 gagacttatg ttgcttggaa tttacacgag cctcgtgaag gtgagtttca ttttgacggt
    11461 gatctggatt tagagaaatt tctccaaata gcgcaggatt tgggtctcta cgcaattgtg
    11521 cgtccgtctc catttatctg tgcggaatgg gaattcggtg gcttaccagc ttggctcttg
    11581 accaagaaca tgcgaattcg ctcatccgac ccagcatata tcgaggcagt tggtcgctac
    11641 tatgatcagt tattgccaag actggtgcct cgtttgttga acaatggtgg caatattctc
    11701 atgatgcagg ttgaaaatga gtatggttct tacggagaag ataaggctta cctgagagcg
    11761 attcgacagc taatggaaga gtgtggcgta acctgtcccc tctttacatc agatggtcca
    11821 tggcgagcta ctctgaaagc tggaacctta attgaagagg acctctttgt aacaggaaac
    11881 tttggttcta aggcacctta caacttttcg cagatgcagg aattctttga tgaacatggt
    11941 aagaaatggc cactcatgtg tatggagttc tgggatggtt ggttcaatcg ctggaaagaa
    12001 ccgattatca cacgggatcc taaggaattg gcagatgcag ttcgagaggt tttggaacaa
    12061 ggctctatca atctttacat gttccacggt ggtacaaact ttggtttcat gaatggttgc
    12121 tcagctcgag gaactttgga cctgccacaa gttacatctt atgattacga tgcccttctg
    12181 gatgaagaag gaaatccaac tgctaaatat cttgcagtca agaagatgat ggcaacacat
    12241 ttttcagagt atccgcagtt ggaaccactc tacaaagaga gtatggagtt ggatgctatt
    12301 ccactagttg aaaaagtttc tttgtttgaa accttagata gcttgtcaag tcctgtagaa
    12361 agtctctatc ctcaaaagat ggaggagctg ggacaaagtt atggctacct actttatcga
    12421 acagaaacaa actgggatgc agaagaagaa agacttcgta tcattgatgg tcgagatagg
    12481 gcccagctgt atgtcgatgg tcagtgggtt aaaactcaat atcagacaga gattggggaa
    12541 gatatttttt atcaaggtaa aaagaaaggg ctatctaggt tagatatctt gatagaaaat
    12601 atggggcgtg tcaactatgg gcataagttc ttagcggata cgcaacgtaa gggaattcgg
    12661 acaggggtct gtaaggatct gcatttctta ctaaactgga aacactatcc actcccacta
    12721 gacaatcctg agaaaattga tttttcaaaa ggatggactc aaggacaacc agccttttac
    12781 gcttatgact ttacagtcga agagccaaaa gatacttacc tagacttgtc tgagtttggt
    12841 aagggagttg cctttgtcaa tgggcagaat ctaggacgtt tttggaacgt tggcccaact
    12901 ctctcacttt atatccctca tagctatctc aaggaaggtg ccaaccgcat cattatcttt
    12961 gaaacagaag gtcaatataa agaagagatt catttaactc gtaaacctac actaaaacat
    13021 ataaaggggg aaaacttatg acaattgtag gatgccgtat tgatggacgt ttgatccacg
    13081 gacaagtagc caatctttgg gctggaaaac taaatgtttc acgcattatg gttgtagacg
    13141 acgaagttgt caacaacgat attgaaaaga gtggtttgaa acttgcgaca ccaccaggtg
    13201 tgaaattgag tattttgcca gttgagaaag ctgcagccaa tattcttgct ggtaaatacg
    13261 atagccaacg tctctttatc gtggctcgta aaccagaccg cttccttggt ttggtagaag
    13321 caggtgtacc acttgaaacc cttaatgttg ggaatatgtc tcaaacacca gaaactcgtt
    13381 ctattacacg ttctatcaac gtagtagaca aggatgtgga agacttccac aaactggcag
    13441 aaaaaggtgt taaacttact gctcagatgg ttccaaatga tccaatttca gactttttga
    13501 gcttattaaa ataggaaaaa aatttttagg aggtcattgt tatgatacaa tggtggcaaa
    13561 ttttacttct cactttgtac tcagcttatc aaatctgtga tgagttgacg atcgtttcat
    13621 ctgcaggttc ccctgtattt gctggtttca ttactggttt aatcatggga gatgtgacta
    13681 ctggtttact tatcggtggt aacttgcaac tgttcgttct tggggttggt accttcggtg
    13741 gtgcttctcg tatcgacgca acttctggtg cggttcttgc gacagccttc tctgtttcac
    13801 aaggaattga tgcaccgctt gccattacta caatcgctgt accagtagct gctctcttga
    13861 cttacttcga cgttcttggt cgtatgacta ctaccttctt cgctcaccgt gtggatgctg
    13921 caatcgaacg ctttgactat aaaggtattg aacgcaacta cttgcttggt gcgattccgt
    13981 gggctctatc tcgtgccctt ccagtcttct ttgcccttgc ttttggtggt gcctttgtac
    14041 aatcagtagt agacttcgtt gaagcctaca aatgggttgc agatggcttg acacttgcag
    14101 gacgtatgct tccaggtctt ggatttgcaa tcttgcttcg ttaccttcca gttaaacgta
    14161 accttcacta ccttgctatg ggatttggtt tgacagctat gttgactgtt ctttactcat
    14221 atgtaacagg tcttggtggc gctgttgctg gtatcgtagg tactcttcct gctgaagttg
    14281 ctgaaaaaat tggtttcgtg aacaacttca aaggtttgtc tatgattggt atttctatcg
    14341 taggtatttt ccttgcagtg cttcacttca aaaatagcca aaaagtagct gtagcagcac
    14401 cttctacacc atcagaaagt ggggaaatcg aagatgacga attctaatta caaacttaca
    14461 aaagaagatt ttaatcaaat caacaaacgt agcttgttta ctttccaatt aggttggaac
    14521 tacgaacgta tgcaagcttc tggttacctt tacatgatct tgcctcagtt gcgtaaaatg
    14581 tatggtgatg gaactcctga attgaaagaa atgatgaaag ttcatactca attcttcaat
    14641 acttcaccat tcttccatac cattatcgct ggttttgacc ttgccatgga agaaaaagat
    14701 ggtgtaggtt caaaagacgc cgttaacggt atcaagacag gtttgatggg accattcgct
    14761 cctcttgggg atacaatctt tggttcactt gtacctgcta tcatggggtc agtcgcagca
    14821 actatggcta tcgctggcca accttggggg atcttccttt ggattgcagt tgcagtagcg
    14881 tatgacatct tccgttggaa acagttggaa tttgcttaca aagaaggggt taaccttatc
    14941 aacaacatgc aaagtacctt gacagctttg attgacgctg catctgtact tggtgtcttc
    15001 atgatgggtg ctcttgtagc aacagtgatt aactttgaaa tttcttacaa gttgccaatc
    15061 ggtgaaaaga tgattgattt ccaagacatc ttgaaccaaa tcttcccacg tttgcttcca
    15121 gcaatcttta ctgcctttat cttctggttg cttggtaaaa aaggtatgaa ctctactaaa
    15181 gctatcggta ttattatcgt acttgctttg gctctttctg cccttggtca ctttgcactt
    15241 ggaatgtaat tccttatgac taaatcatta attttggtga gccatggtcg cttctgtgag
    15301 gagcttagag gtagcacaga aatgattatg ggcccacaag acaacattta cacagtagct
    15361 cttcttccag aagatggccc agaagaattt actgctaaat ttgaagctgt tattgaaggg
    15421 ttggatgatt tcctagtctt tgcggatctt ctcggtggga caccttgtaa tgtggtgagt
    15481 cgcttgatca tggaaggtcg tgatattgac ctttacgcag ggatgaatct tccaatggtg
    15541 attgaattta tcaatgcgag ccttacaggc gcagatgcgg actacaagag ccgtgctgca
    15601 gaaagcattg tgaaagttaa tgacctgtta gcgggcttcg atgatgacga agatgaataa
    15661 tactcttcga aaatctcttc aaactacgtc aacgtcgcct tgccgtaggt atatgttact
    15721 gacttcgtca gtcttatccg gcaacctcaa aacggtgttt tgagctgact tcgtcagttc
    15781 tatctgcaac ctcaaagcag tgctttgagc agcctgcggc tagtttccta ttttagttgg
    15841 aactcgattc aattcatgtg acaacgtgaa aatcgttaga gcattttata tagaatatac
    15901 atgggaatgt agcttactcc cattcccata tttaatagaa aaagaggaac tcaatgctac
    15961 attatacaaa agaagacttg ctcgaattgg gtgcagaaat cactacgcgt gaaatctacc
    16021 aacagcctga tgtatggaga gaagcttttg aattttatca agcaaaacgt gaagaaattg
    16081 cagccttcct acaagaaatc gctgataaac atgactatat taaggttatc ttgacaggtg
    16141 ctgggacttc tgcttatgtg ggagacacct tgctacctta ctttaaggaa gtctatgacg
    16201 aacgcaaatg gaatttcaat gctattgcga caacagatat cgttgccaat ccagcaacct
    16261 atttgaaaaa agatgtggca actgtccttg tgtcttttgc tcgtagtggg aactcgcctg
    16321 aaagtgtggc gactgttgat ttggccaaat ccttggtgga tgagctttat caagtgacga
    16381 ttacttgtgc agcagatggt aaattggctc ttcaagctca cggtgatgac cgtaatctct
    16441 tgctcttgca accagctgtc tctaatgatg ctggatttgc catgacttct agctttacgt
    16501 ctatgatgtt gacagctctc ttggtctttg atcctacaga atttgctgtt aagtctgaac
    16561 gttttgaagt tgtatctagt cttgcccgta aagttttaga caaggcagaa gatgtcaaag
    16621 agctcgttga tttagacttt aaccgtgtca tctatctagg cgctggtcct ttctttggac
    16681 ttgctcatga agctcagctc aagattttgg aattaactgc tggtcaagtt gcgaccatgt
    16741 atgaaagccc agttggcttc cgtcacggtc caaaatctct tatcaacgac aatacagttg
    16801 ttttggtctt tggtacaacg acggactaca ctcgtaagta cgacttggac ttggttcgtg
    16861 aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt gagtgatcaa gcttttggtc
    16921 ttgaaaatgt caaagaagtg gcccttggtt gtggcggtgt cttgaatgat atttaccgtg
    16981 tcttccctta catcgtttat gcccaactct ttgccctatt gacttcactc aaggtagaaa
    17041 ataaaccaga tacaccgtct cctacaggta cagtaaaccg tgtagtacaa ggtgtcataa
    17101 ttcacgaata tcaaaagtaa gacagtgttt atgaattctt gacaagagga tttgtaaatt
    17161 atcagataaa ccatagattg tcagtacgct ttctatggtt tgtttgcttg agagaaatag
    17221 taaaaggaga acagaatgaa agcatacaca gagcgtgtat ttggaaatgt tgagggcgag
    17281 gatgtcttgg cctatcgatt tgagacagac ggtggctacc aacttgaggt tatgacttat
    17341 ggtgcgacca tcttgcgcta tgtcgcacct gacaaggctg gaaattttgc caatgttatc
    17401 ttgggatttg atgactttga tagttatgta ggcaatagtc ccaagcatgg agcaagtgta
    17461 ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc tcaatggtaa gacctatgac
    17521 cttgaggtta ataatgctag caactgtaat cacagtggtt caactggttg ggattccagc
    17581 ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc tctacacaga gcgtacagat
    17641 gggacaggag gattccctgg aaatctcaag atttggatca gttatcactt ggaagaaact
    17701 ggtgcctatg aaatcagcta caaggtaacg accgatcagg atacgctggt caatccaacc
    17761 aaccacagct atttcaactt gtctggtgat ttcacgcaga cgattgaccg tcatgtcttc
    17821 caactaaaca cagagggcat ttactcaatc gctcctgacg gtgttcctgc caaaactcca
    17881 gaagccaacc gtgatgtggt caaacacatc tacaatggtg ccttgttgaa ggatatcttt
    17941 gcagaagaag atgagcaaat ccagctggca tcaggtttgg atcatccatt tgcccttcct
    18001 gcaggccatg acaatgctgg attcctttat gaccaaaatt caggtcgctt cctgcttttc
    18061 aagacagaag ctccttgctt tgtggtctac acagcaaact ttgtggatga aagtgtcatc
    18121 ataggaggtc agccaatgct agagcacaat gggattgctc ttgaagcgca agctttacca
    18181 gatgccattc acagtgacct taaaggccaa gtcattctta aagctggtca aaccttcacc
    18241 agtaagacac gttatgaact tgttgtgaaa taaaaaagct ggtcaaaaag tctttaaatt
    18301 aaaataatgc atattgtcag gtgatggaaa gtttgatact atgcgtttta ttatgaaatg
    18361 atttacttta tcttatcctg aaatggtgtt atttttgagt tatctggctt gactttcttg
    18421 atgaaaatta tataatagtt gcaacaataa taattacaac gagaaaagag gaccatttat
    18481 gacctatgaa tacaagagtc acatttattt ggcagagaca gctttaaatg taaaggattt
    18541 ggccagtcaa acagcctttt atcagcaagt tattggttta gaaatcttat ctcaaactga
    18601 gacagagtcc attctaggcc ttggtggaaa agtcttggta cagctgattc aagcacaaga
    18661 gagcggagaa gtgagggaac attatggtct ttaccatttg gccattctct tgcccacacg
    18721 caaggctttg gctgatgtct tgaaacacct gacagattta cagattcctc ttgtcggcgg
    18781 tgcagaccat ggttacagtg aagctattta cttggaagat ttggagggaa atggcattga
    18841 actctatcga gataagccag tttccacatg ggatattcga gaagatggac gcattatcgg
    18901 agtgacagag gctcttgcgg cgcaggacat ttatgagttg ggggaaagag tagagccctt
    18961 tatcctggca gaaggtacga gaatggggca tattcatctt tccgtcaagg atagtcgaaa
    19021 gtccagccag ttttatcaaa aggtgttagg gctagaggat aaattcagtg tgcctggcgc
    19081 tagttggatt gcggctggag attaccatca tcatttagca gttaacgaat ggggaggaaa
    19141 aggtctggct tcgcgtaagc aaggcttacc aggtttagcc tactatgtca tcgaagtcgc
    19201 acataaaaaa gaactattaa cgattgccca acgagcacaa gaagttgacg caccaatcaa
    19261 atggatgaca tcgagccaat tggaaatcac agactcagat ggcatcgtga cccgtattcg
    19321 tttagcaagg taaaattagg aatttttaat aagttataag tattgctagt ttgctttttg
    19381 aacggtcttt ggtaacagaa ctgcgtgagc tggatgggcg agaattttga caaagaagct
    19441 gattagagat ataatgaaga aaaatcgagc aaggaaaaag aaatgacaaa ttcaaagtat
    19501 attacttgtt tgaaacgttc agagggccag ttgtgtagga ttcaaaagat gattgaagga
    19561 gatcgtgact gtgctgatat tgtgacgcaa ctgacagcag tgagatctag tgtagagcgc
    19621 gtgattgaga tgataattac cgaaaatctt actgaatgta tcaatcagcc actagatgat
    19681 tctgaagctc aaaaggaacg cctagaaaag gctatccgat acttgattaa acggaaataa
    19741 atgaatgttt tgagacgata tgtgcaaagg tctattaact attagaatag tttaatgtga
    19801 gtcaaaagaa gttgtaaaaa atctttaaaa ttaaaaaaac gcatagtatc aggtgttgaa
    19861 tgtactgacc cccaaaagtt agattttttc tgtctaactt ttggggtgca gttcataaga
    19921 accttggtaa tatgcgtttt attatgtaga gacttatact cttcgaaaat ctcttcaaac
    19981 cacgtcagct tcaccttgcc gtagatatgg ttactgactt cgtcagttct atccacaacc
    20041 ttaaaacagt gttttgagca gcctgcggct aacttcctag tttgcacttt gattttcatt
    20101 gagtattact tcatcttttc ctgaaattga gtttttgccc aactcctctt gattattcta
    20161 cttgaccggg ccaagcattc ataccacctt ctacgttggt aacggtgagg ccttgggagc
    20221 tgagaaactg gcaggtagat gcagagcgga ctcctccgtg acaaatgaca tagtattcat
    20281 ggtcaggttt gagttctttg tagccttgct ctaaggtatt tatggaagat ttttgggtcc
    20341 tgcttggaat tcatgtgctt cacggacatc gataagttcc agattttcat tttgatattt
    20401 tccataaaag tcagccatgc tgatactagt ttccatattc tactccttct gggttagcca
    20461 ttttgtatag tgaataagcg ccgtcaaggt tttggacggt aaatcctgct tgcttgagga
    20521 taggctctgc gatatagcta cgcaaaccac tgtggcagct gacgatgtag gcttgttttt
    20581 tgtccagttc atcagcttcc tccttgacac tcggtcagat tttgatacaa tagtacaaaa
    20641 ttagaggagg tgggctatga ttcagaaaca tgcgattccc attttagagt ttgatgataa
    20701 tcctcaggcg gttatcatgc ccaatcacga ggggttggat ttgcagttgc caaagaagtg
    20761 tgtctatgct tttttaggtg aggagattga ccgctatgcg agggaagtag gggcggattg
    20821 tgtcggcgaa tttgtttctg ccaccaaggc ctatccagtt tatgtcgtga actacaagga
    20881 cgaggagatt tgtctggttc aggctcctgt cggttcagct ccagcagccc agtttatgga
    20941 ttggttgatt ggctatggtg tggagcagat tatctctact gggacctgtg gtgtcctagc
    21001 tgatatagag gaaaatgcct ttctagtccc tgttcgcgct ctgcgagatg aaggagccag
    21061 ttaccactat gtggcacctt ctcgttatat ggaaatgcag ccagaggcta ttgctgctat
    21121 tgaggaagtt ttggaagaca gagggattcc ttatgaagaa gtcatgacct ggtccacaga
    21181 tggtttttac cgagaaacgg ctgaaaaggt ggcttatcgc aaggaagaag actgtgctgt
    21241 tgtggagatg gagtgttccg ctcttgcggc agtagctcaa ttgcgtgggg ttctctgggg
    21301 tgaattgttg ttcacagcag attctctagc ggacttggac cagtacgaca gtcgtgactg
    21361 gggctcggaa gcttttaata aggcgctaga actgagttta gcaagtgttc accaccttta
    21421 gttgtactgg caaaggattt gttttatcat aaaatgtcta gctcatactt ttcaaaaata
    21481 tgtttaaacg aggtcacctt cctcttgtcc taggcatgtt gaggttggga aaaatcttta
    21541 aaatcagaaa aacgtatcat atcaggtgat gaaaactttg atactatgcg ttttatgttg
    21601 gtaagattta gagtgagatg aaatgatact cttcgaaaat ctcttcaaac caggccagct
    21661 tcaccttgcc gtatagatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg
    21721 ttttgagcaa cctgtgacta gctttctaag cgatgccttg gttctcattg cctataatca
    21781 aaaagagaaa ttttctcctg aaaagcatat agagtagctg gcgttaaaag ctcctgtctt
    21841 gcttttttga cctatagtca catctatcaa gtattgttct tgcctaagct atcaataaaa
    21901 aggtggcatt ttttaggctt ggtgttagtg aattttgcct tatcctatct aagtcatttc
    21961 gagcttttta tggtacaatg gaaacatgtt attcaaatta tctaaggaaa aaatagagct
    22021 aggcttatct cgtttatcgc cagcccgtcg tatttttttg agttttgcct tggtcatttt
    22081 actaggctct cttcttttga gcttgccctt tgtccaagtt gaaagctcac gagcgactta
    22141 ttttgatcat cttttcactg ctgtctctgc agtctgtgtg acaggtctct caacccttcc
    22201 agtagctcac acctataata tatggggcca aataatctgt ttgctcttga ttcagatcgg
    22261 tggtctaggg ctcatgacct ttattggggt tttctatatc cagagcaagc aaaagcttag
    22321 tcttcgtagc cgtgcaacta ttcaggatag ttttagttat ggagaaactc gatctttgag
    22381 aaagtttgtc tattctattt ttctcacgac ctttttggtt gagagcttgg gagctatttt
    22441 gcttagtttt cgccttattc ctcaacttgg ctggggacgt ggtcttttta gttccatttt
    22501 tctagcgatc tcagccttct gtaatgccgg ttttgataat ttagggagca ccagtttatt
    22561 tgcttttcag accgatttac tggtcaatct ggtgattgca ggcttgatta ttacaggcgg
    22621 ccttggtttt atggtctggt ttgatttggc tggtcatgta ggaagaaaga aaaaaggacg
    22681 tctgcacttt catacgaagc ttgtactatt gttgactata ggtttgttgt tatttggaac
    22741 ggcaactact ctctttcttg agtggaacaa tgctggaacg attggcaatc tccctgttgc
    22801 cgataaggtt ttagttagct tttttcaaac agtgacgatg cgaacagctg gcttttctac
    22861 gatagattat actcaggctc atcctgtgac tcttttgatt tatatcttac agatgtttct
    22921 aggtggggca cctggaggaa cagctggggg actcaagatt acgacatttt ttgtcctctt
    22981 ggtctttgca cgaagtgagc ttctaggctt gcctcatgcc aatgttgcga gacgaacgat
    23041 cgcgccgcga acggttcaaa aatcctttag tgtctttatt atctttttga tgagcttctt
    23101 gataggattg attctgctag ggataacagc caaaggcaat cctcccttta tccacctcat
    23161 atttgaaacc atttcagctc ttagtacagt tggtgtaacg gcaaatctga ctcctgacct
    23221 tgggaaattg gctctcagtg ttatcatgcc acttatgttt atgggacgaa ttggtccctt
    23281 gaccttgttt gttagcttgg cagattacca tccagaaaag aaagatatga ttcactatat
    23341 gaaagcagat attagtattg gttaagaaag gaaagatcat gtcagatcgt acgattggaa
    23401 ttttgggctt gggaattttt gggagcagtg tcctagctgc cctagccaag caggatatga
    23461 atattatcgc tattgatgac cacgcagagc gcatcaatca gtttgagcca gttttggcgc
    23521 gtggagtgat tggtgacatc acagatgaag aattattgag atcagcaggg attgatacct
    23581 gcgataccgt tgtagtcgcg acaggtgaaa atctggagtc gagtgtgctt gcggttatgc
    23641 actgtaagag tttgggggta ccgactgtta ttgctaaggt caaaagtcag accgctaaga
    23701 aagtgctaga aaagattgga gctgactcgg ttatctcgcc agagtatgaa atggggcagt
    23761 ctctagcaca gaccattctt ttccataata gtgttgatgt ctttcagttg gataaaaatg
    23821 tgtctatcgt ggagatgaaa attcctcagt cttgggcagg tcaaagtctg agtaaattag
    23881 acctccgtgg caaatacaat ctgaatattt tgggtttccg agagcaggaa aattccccat
    23941 tggatgttga atttggacca gatgacctct tgaaagcaga tacctatatt ttggcagtca
    24001 tcaacaacca gtatttggat accctagtag cattgaattc gtaaagaggg atgacccctc
    24061 ttttttgatg cccaagatgg caaatagaga cagaagcccc ttgtcttcta gttaaaagtt
    24121 cttcaaaggc tggactttat gataaaatag aaagaagtga caagagagag taatactcaa
    24181 tgaaaatcaa agatcaaact aggaaactag ctgcgggctg ctcaaaacac tgttttgagg
    24241 ttgtggatag aactgacgaa gtcagctcaa aacactgttt tgaggttgca gatagaactg
    24301 acgaagtcag taacatctat acggcaaggc gacgttgacg cggtttgaag agattttcga
    24361 agagtataag aaaaaatcag tcccctaaag gagcagatta tgaagttatt gtctatcgcc
    24421 atttctagct ataatgcagc agcctatctt cattactgtg tggagtcgct agtgattggt
    24481 ggtgagcaag ttgagatttt gattatcaat gacgggtctc aggatcagac tcaggaaatc
    24541 gctgagtgtt tagctagcaa gtatcctaac atcgttagag ccatctatca ggaaaataaa
    24601 ggccatggcg gtgtggtcaa ttgcggtttg gcagaggctt ctgggcgcta ttttaaagta
    24661 gttgacagtg atgactgggt ggatcctcgt gcctacttga aaattcttga aaccttgcag
    24721 gaacttgaga gcaagggtca agagatggat gtctttgtga ccaattttgt ctatgaaaag
    24781 gaaggtcagt ctcgtaagaa gagtatgagt tacgagtcag tcttgcctgt tcggcagatt
    24841 tttggctggg accaggtcgg aaatttctcc aaaggccagt atatcatgat gcactcgctg
    24901 atttatcgga cagatttgtt gcgtgctagc cagttctaac tgcctgaaca taatttttat
    24961 gtcgataatc tctttgtctt tacgcccctt cagcaggtca agaccatgta ctatctgcct
    25021 gtcgatttct atcgttattt gattgggcgt gagggccagt ctgtcaatga gcaagtgcat
    25081 tgaccagcaa ctcaaggtca atcgactctt ggtagaccag cttgatttgt cccaagtgag
    25141 tcatcccaaa atgcgagaat atctgctgaa tcatattgaa atcacgacgg tgatttccag
    25201 taccctgctc aaccgatctg aaacagcgga gcatctggca aaaaaacgcc aattgtggac
    25261 ctatattcag cagaaaaatc cagaagtctt tcaggctatt cgtaagacca tgttgagccg
    25321 tttgaccaaa cattctgtct tgccagatcg caaactgtcc aatgtcgtct atcaaatcac
    25381 caaatctgtt tatggattta attaatataa gtgttttata agagggattt aagaaaaatt
    25441 ttaacttttt cttagtcctt tttaatttca ggagattata ctagagtcat caaataaaga
    25501 aagactctaa ggagaatcct atgaaattca atccaaatca aagatatact cgttggtcta
    25561 ttcgccgtct cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc tttgtcctag
    25621 ttggtcagcc aagttctgta cgtgccgatg tggtcaatcc gaccccaggt caagtcttac
    25681 ctgaagagac atcgggaacg aaagagggtg acttatcaga aaaaccagga gacaccgttc
    25741 tcactcaagc gaaacctgag ggcgttactg gaaatacgaa ttcacttccg acacctacag
    25801 aaagaactga agtgagcgag gaaacaaact cttctagtct ggatacactt tttgaaaaag
    25861 atgaagaagc tcaaaaaaat ccagagctaa cagatgtctt aaaagaaact gtagatacag
    25921 ctgatgtgga tgggacacaa gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg
    25981 gagtgaaaga aaatacaaaa gacagcatcg atgttcctgc tgcttatctt gaaaaagctg
    26041 aagggaaagg tcctttcact gccggtgtaa accaagtaat tccttatgaa ctattcgctg
    26101 gtgatggtat gttaactcgt ctattactaa aagcttcgga taatgctcct tggtctgaca
    26161 atggtactgc taaaaatcct gctttacctc ctcttgaagg attaacaaaa gggaaatact
    26221 tctatgaagt agacttaaat ggcaatactg ttggtaaaca aggtcaagct ttaattgatc
    26281 aacttcgcgc taatggtact caaacttata aagctactgt taaagtttac ggaaataaag
    26341 acggtaaagc tgacttgact aatctagttg ctactaaaaa tgtagacatc aacatcaatg
    26401 gattagtt
//
LOCUS       DAVGJJ010000021        25994 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000021 DAVGJJ010000000
VERSION     DAVGJJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.021"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            422..1585
                     /locus_tag="VVE35_000792"
     CDS             422..1585
                     /locus_tag="VVE35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU3061021.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1582..2259
                     /locus_tag="VVE35_000793"
     CDS             1582..2259
                     /locus_tag="VVE35_000793"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU3061022.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2249..3103
                     /locus_tag="VVE35_000794"
     CDS             2249..3103
                     /locus_tag="VVE35_000794"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU3061023.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3122..4189
                     /locus_tag="VVE35_000795"
     CDS             3122..4189
                     /locus_tag="VVE35_000795"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU3061024.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4199..5365
                     /gene="aroC"
                     /locus_tag="VVE35_000796"
     CDS             4199..5365
                     /gene="aroC"
                     /locus_tag="VVE35_000796"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU3061025.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5375..6478
                     /locus_tag="VVE35_000797"
     CDS             5375..6478
                     /locus_tag="VVE35_000797"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU3061026.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6489..6827
                     /locus_tag="VVE35_000798"
     CDS             6489..6827
                     /locus_tag="VVE35_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU3061027.1"
                     /translation="MSNIYDSANELSRGLRGLSEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6919..8202
                     /gene="aroA"
                     /locus_tag="VVE35_000799"
     CDS             6919..8202
                     /gene="aroA"
                     /locus_tag="VVE35_000799"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU3061028.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDSKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8195..8671
                     /locus_tag="VVE35_000800"
     CDS             8195..8671
                     /locus_tag="VVE35_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU3061029.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDTNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8668..9516
                     /gene="pheA"
                     /locus_tag="VVE35_000801"
     CDS             8668..9516
                     /gene="pheA"
                     /locus_tag="VVE35_000801"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU3061030.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFDNITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9513..10787
                     /gene="psr"
                     /locus_tag="VVE35_000802"
     CDS             9513..10787
                     /gene="psr"
                     /locus_tag="VVE35_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU3061031.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11066..11911
                     /locus_tag="VVE35_000803"
     CDS             11066..11911
                     /locus_tag="VVE35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3061032.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDTVIADMDKSYTEYK
                     GEYGG"
     gene            11901..13004
                     /locus_tag="VVE35_000804"
     CDS             11901..13004
                     /locus_tag="VVE35_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3061033.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGVGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13006..13992
                     /locus_tag="VVE35_000805"
     CDS             13006..13992
                     /locus_tag="VVE35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3061034.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKELIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14008..15441
                     /locus_tag="VVE35_000806"
     CDS             14008..15441
                     /locus_tag="VVE35_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU3061035.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLTRYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15470..16504
                     /locus_tag="VVE35_000807"
     CDS             15470..16504
                     /locus_tag="VVE35_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU3061036.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16704..17441
                     /gene="mecA"
                     /locus_tag="VVE35_000808"
     CDS             16704..17441
                     /gene="mecA"
                     /locus_tag="VVE35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU3061037.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17592..18878
                     /locus_tag="VVE35_000809"
     CDS             17592..18878
                     /locus_tag="VVE35_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU3061038.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNHLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKAHV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18880..19749
                     /locus_tag="VVE35_000810"
     CDS             18880..19749
                     /locus_tag="VVE35_000810"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU3061039.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19999..20937
                     /gene="msrB"
                     /locus_tag="VVE35_000811"
     CDS             19999..20937
                     /gene="msrB"
                     /locus_tag="VVE35_000811"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU3061040.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20995..22719
                     /locus_tag="VVE35_000812"
     CDS             20995..22719
                     /locus_tag="VVE35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061041.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLIPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22721..24469
                     /locus_tag="VVE35_000813"
     CDS             22721..24469
                     /locus_tag="VVE35_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061042.1"
                     /translation="MKRQTVNQTLKRLAVDLANHSFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24726..25985
                     /locus_tag="VVE35_000814"
     CDS             24726..25985
                     /locus_tag="VVE35_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU3061043.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIMDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
BASE COUNT         7657 a         4862 c         5869 g         7606 t
ORIGIN      
        1 aataggtgaa attttaatta ttgttgcttc tataatatta tttattgata gtatattgag
       61 tttgtaacaa aaggcttttc tataaaatca aattttccag atatctactg aagctgattt
      121 atttcttcat ctttcttcta ctacctttgt cactaaattt gacagacaca tattgctgat
      181 acaacctctc tacctataat ctcacggtta acctgaggct agtttcatag tttgctattt
      241 gattttcgtt gataaaaaga aatgctcctc caagacggaa gagcattttt tactgtgatt
      301 cttacacatt tacttcctag ttttgcagga agtctagtat accatttttt aaaagaagag
      361 taaaaaagca agggtagttt cttttttgag aattgtctaa aagtttgata taatggtagt
      421 tatgaataga ataagagtca gcaaaagggt tgaaaagaag cttgctaagg ggctagtttt
      481 actagaagcc agtgatcttg agaatgtcaa tcttaaggat caggaagtag aggtgcaggg
      541 tcaggaagga aactttcttg ggactgccta cctttctcag caaaacaagg gcttgggctg
      601 gtttatcagc aaagacaagg tggccttcaa tcaagctttc tttgaaacgt tgtttagaaa
      661 agccaaagaa aagagaaacg cctactatca agatgatttg acaactgcct ttcgtctttt
      721 taatcaagag ggagatggct ttgggggtct gacagtggac ctttatggcg actacgccgt
      781 cttttcttgg tataactctt atgtttatca gattcgtcag actatatcag aagcctttag
      841 acaggttttc cctgaggttt taggagctta tgagaaaatc cgctttaagg gtttggacta
      901 tgaatctgcc catgtttatg gtcaagaagc acctgacttt ttcactgttt tagaaaatgg
      961 tgtcctgtat caagtcttta tgaatgatgg cttgatgaca ggaattttcc tagaccagca
     1021 tgaggttcgc ggtagtttag ttgacggctt ggctatgggt aaatccttgc tcaatatgtt
     1081 ttcctacaca gcggcttttt cagtagctgc ggccatggga ggagctagcc agacaacttc
     1141 tgttgatcta gccaaacgtt cacgagaatt gtctcaagcg cattttcagg caaatgggct
     1201 cagcacagac gagcatcgtt ttatagtcat ggatgtcttt gagtatttca aatatgccaa
     1261 acgcaaagac ttgacctacg atgtgattgt cctagatccg cctagctttg ctcggaataa
     1321 aaaacaaact ttctctgtgg ccaaggatta tcacaagttg atttcccaga gtcttgagat
     1381 tttaaatccg ggagggatta tcattgccag taccaatgct gccaatgttt cccgtcagaa
     1441 atttacagaa caaattgata aaggttttgc aggaagaagt taccagattt taaacaaata
     1501 tggtcttcca gcagattttg cctataataa aaaagatgaa agtagtaatt acctcaaggt
     1561 gattagtatg aaggttagta aatgaaatta atcgtttcag taatgccaag aagtttagag
     1621 gaggctcagg ctctggatgc cacgaggtac ctggatgccg acatcattga atggcgtgcc
     1681 gactatctgc ctaaagaagc gattttgcag gtagctccag ccatttttga aaaattcgca
     1741 ggccgtgagt tggttttcac gctacgaact cgctccgaag gtggagaaat cgacctttct
     1801 ccagaagagt atatccatct aatcaaggaa gttgcgcaac tctatcaacc agactatatt
     1861 gattttgagt actatagcta caaggatgtt tttgaggaaa tgctggactt cccaaatctt
     1921 gttttgagtt accataattt ccaagaaaca cctgagaaca tgatggaaat cttgtcagag
     1981 ttgacgatcc taaatccaaa acttgttaag gttgcggtga tggctcacac ggagcaggat
     2041 gtcttagact tgatgaacta tacacgaggc tttaaaaccc tcaatcctga acaggaatat
     2101 gtgaccattt ctatgggtaa ggtgggcaag gtctctcgta tcactgcgga tgtgactggt
     2161 tcgagttggt cttttgccag tctggatgag gttagtgccc ctgggcagat ttctctagct
     2221 agcatgaaaa aaatcaggga gattttggat gaagcttgat ggctatacac gtttagctgc
     2281 agttgttgcc aatcctatta agcattctat ttctcccttc atccacaata gagcctttga
     2341 ggcgacagct accaacggtg cttatgtggc ttgggagatt gaagcgagtg acttggcaga
     2401 aacagtggcc aatattcgtc gctaccagat gtttggcatc aatctgtcca tgccctataa
     2461 ggagcaggtg attccttatt tggatgagct aagcgatgaa gcgcgcttga ttggtgcggt
     2521 taatacggtt gtcaatgaga atggcaattt aattggatat aatacagatg gcaagggatt
     2581 ttttaagtgc ttgccttctt ttacaatttc aggtaaaaag atgaccctgc tgggtgcagg
     2641 tggtgcggct aaatcaatct tggcacaggc tattttggat ggcgtcagtc agatttcggt
     2701 ctttgttcgt tccgtttcta tggaaaaaac aagaccttac ctagacaagt tacaggagca
     2761 gacaggcttt aaagtggatt tgtgtgcttt agaatatgtt tctgaactgc aagcaaggat
     2821 tgccgagtcg gatttgctag ttaatgccac cagtgtgggc atggatggcc aatcctctcc
     2881 tgttcctgaa aacatagtct taccagaaac tctcttagtt gcagatatca tataccaacc
     2941 ctttgaaaca ccgtttttga aatgggctag aagtcagggc aatccagcag tcaatggtct
     3001 gggaatgttg ctctatcaag ctgcagaagc ttttcaactg tggacaggca aggaaatgcc
     3061 gacagaagag atttggcagt ctttaacaga aaaataccaa taaagaaagg gagactctcc
     3121 tatgaaaatc agaatcgata ttcctcatca tccttatgat attcagattg aaaaaggttg
     3181 tatggcccag gctggtcagt ggttgcgaga actctggcaa ccgcaaaagg tagtcattgt
     3241 gacagataac catgtagcct ctctctatgc agagaaggtc aagctcagcc tagaagatgc
     3301 tggttttcag gtagctgttt ttgatttctt agaaggtgaa gaaagaaaga atttaactac
     3361 tgttcagaaa gtctatgaat ttctagtcaa gcaaggtctg actcgtagcg atggaatcgt
     3421 tgctcttggt ggtggcgttg ttggggacct ggctggcttt gtagcctcta cctatatgcg
     3481 gggtattcac tttgttcaga ttccgactag tttgacagct caggttgatt cttctatcgg
     3541 tggaaagaca ggcgttaaca caccatttgc taaaaatatg gtggggacct ttgcccaacc
     3601 agatggggtt ctgattgatc cacttgttct tgaaaccctc ggaaaaagag agttgattga
     3661 agggatgggt gaagttatca agtatggctt gattgaggat ccagaactgt gggctctctt
     3721 gacgggactg aatggttctg ttgagagtat tttggaacat gcagagacct tgattgaaca
     3781 ttcttgtcag gtgaagcgca agatggtggt tgaagatgag ttggacaatg gtattcgtct
     3841 ttacctcaat tttggccaca ctattggcca tgccatcgaa gcgactgccg gttatggcaa
     3901 ggtcatgcat ggagaggctg ttgccatggg aatggtacag atttccaagg ttgctgagga
     3961 aaaaggcctc atgccagctg gcattaccca atctatcaca gagatgtgtc agaaattcgg
     4021 cttgcctgtt gactatgaaa attgggaagt tgacaagctt tatcaggctc ttactcatga
     4081 caagaaagcg cgtggtaaca ccttgaaatt ggtcttggtg ccagagcttg gttcagcgac
     4141 cattcatcca gtttctctgg aagagatgaa agactacttg gtaaaataag gagagtgtat
     4201 gagatattta actgcaggag aatcacacgg cccccgtcta acagctatta ttgagggaat
     4261 tccagctgga cttccattga cagctgagga tatcaatgag gaccttagac gccgtcaggg
     4321 tggctacggt cgtggtggtc gtatgaagat tgagagtgac caggttgtct ttacttcggg
     4381 cgttcgccac gggaagacga caggggcgcc tattactatg gatgtcatca ataaggacca
     4441 ccagaaatgg ctggacatca tgtctgcgga ggacattgaa gaccgcctta aaagcaagcg
     4501 gaaaattact catcctcgcc caggtcatgc cgatttggtt ggggggatta agtaccgttt
     4561 tgatgatttg cgaaattctt tggagcgttc atcagctcgt gaaaccacca tgcgggtggc
     4621 agttggtgca gtagccaaac gcctcttggc tgagctggat atggagattg ccaaccatgt
     4681 cgtggtcttt ggtggtaagg aaatagatgt tcctgaaaat ctgacagtcg ctgaaattaa
     4741 gcaaagagct gcccagtctg aagtttctat tgtcaaccaa gaacgagaac aggaaatcaa
     4801 ggactatatt gaccaaatca aacgtgatgg tgataccatc ggtggggttg tggagacagt
     4861 cgtcggaggc gttccagttg gtcttggttc ctatgtccaa tgggatagaa aattggatgc
     4921 aagattggct caagctgttg tctctatcaa tgcctttaaa ggggtggaat ttggtcttgg
     4981 ctttgaggct ggttatcgta aaggcagcca agttatggat gaaattctct ggtctaaaga
     5041 agacggttat actcgccgta ccaataatct aggtggtttt gaaggtggta tgactaatgg
     5101 gcaacccatc gttgttcgtg gggtcatgaa acccattcct actctttata aacctcttat
     5161 gagtgtggat atcgaaaccc atgaacctta caaggcaacc gtggagagaa gtgatccgac
     5221 tgctcttcca gctgcaggaa tggtcatgga agcagttgta gcaacggttc tggcgcaaga
     5281 aatcctcgaa aaattctcat cagataatct tgaggaacta aaagaagcgg tagccaaaca
     5341 ccgagactat acaaagaact attaaggagt tcctatggca aaaacaatct atatcgcagg
     5401 tcttgggttg attggagcct ctatggcact tggtatcaaa cgcgatcatc cagattatga
     5461 aattttaggt tataatcgta gtcaagcttc gagagatatc gccttgaaag aaggcatgat
     5521 tgaccgtgca acggatgatt ttgctagttt tgctcctttg gcagatgtca ttatcctcag
     5581 cttgccaatc aaacaaacta ttgctttcat taaggagttg gccaatttgg atttgcgaga
     5641 aggcgttatt atttcagatg ctggttcgac caagtcaacc attgtggatg cggcggagca
     5701 gtatttggct ggcaagtctg ttcgctttgt cggggcccat cccatggctg gtagtcacaa
     5761 gacaggggct gcttcggcag atgtcaatct ttttgaaaat gcctattata tctttacacc
     5821 ttcaagcctg acaagtcagg acacgcttaa ggaaatgaag gatctgcttt caggtcttca
     5881 tgctcgtttt atcgagattg atgccaagga gcatgatcgt gtcacttctc agattagcca
     5941 ttttcctcat attttggctt ctagtctcat ggagcagact gcggtctatg ctcaagagca
     6001 tgagatggca aggcgctttg cggcaggtgg ttttcgagat atgacccgaa ttgcggaaag
     6061 cgagccagga atgtggacct ccattctctt gtccaatagc gagaccattc tggatagaat
     6121 tgaggatttc aaggaacgtt tggaagcgat tggtcaggcc attagtaagg gagatgaaga
     6181 gcaaatttgg aactttttta accaagcgcg tgaacaacgc cagaccatgg aaatccataa
     6241 gcgtggtggt gtggatagct cttacgacct ctatgttgac gttcctgatg aagaagatgt
     6301 catcctgcgg attttggaac tgctacgtgg aacttccttg gttaacatcc acatcaacga
     6361 ggaaaatcgt gaggatattc acggaatcct acaaatttca tttaaaaatg ctcaagactt
     6421 ggaaagagct gagcatctca taacagaaaa taccgactat acagtcgtca tcaaataagg
     6481 agaagaacat gtcaaatatt tacgatagtg caaacgaact cagtcgcggt ctacgcggat
     6541 tatcagaata caaggctgtt aaagcagcta aagatgcgat tgcagcagat gctgaggcaa
     6601 gcaaaatctt tacagaatat cttgcctttc aagaggaaat tcaaaaacta gcacagacag
     6661 gtcaaatgcc agatgcgtcc ttccaagcta agatggaagg ctttggtaaa cagattcaag
     6721 gaaatagcct cttgtcagaa ttctttacca agcaacaaca attggccatt tacctttctg
     6781 acattgaaaa aattgttttt gaaccagttt cagaattgct gaaataagaa aagaacgatc
     6841 ctaaagtcag gaatttttaa ggaatttctg gctttttatg ataaaatagg taaaagtatt
     6901 gcaagtatga ggtccagtat gaaactaaaa acaaatattc gccatttaca tggtagtatc
     6961 cgcgtcccag gtgacaagtc tatcagccac cgttccatta tctttggaag tttggctgag
     7021 ggtgagacca aggtttatga tattctgcga ggtgaagacg ttctttcgac catgcaggtt
     7081 tttcgtgacc ttggtgttga aattgaggat aaagatgggg ttattaccat tcaaggtgta
     7141 ggcatggctg gcttaaaagc gccgcaaaat gcccttaata tgggaaattc tggcacctcg
     7201 attcgcctga tttcaggtgt ccttgctggt gcagatttcg aagtagagat gtttggagat
     7261 gatagtcttt ccaaacgtcc tatggaccgt gtgacccttc cactgaaaaa aatgggcgtc
     7321 agcatttcag ggcaaactga acgagacttg cctccccttc gcttaaaagg gacgaaaaac
     7381 ctaagaccta ttcattatga gttgccaatt gcctctgccc aagtcaagtc agccttgatg
     7441 tttgcagcct tacaagctaa gggggagtca gttattatcg aaaaagagtg cacccgtaat
     7501 catactgaag atatgttgaa acaatttggt ggtcatttaa gtgtggacag taagaaaatc
     7561 acagtccaag ggccacaaaa attgacagga cagaaggtgg tcgtaccagg agatatttcc
     7621 agtgcagcct tttggttagt cgcaggtttg attgttccaa attctcgtct agtgctgcag
     7681 aatgtgggga tcaacgaaac tcgcaccggt attattgatg tcattcgtgc catgggtgga
     7741 aaattggaaa taactgaaat cgatccagtc gctaaatctt ctaccttgac tgttgagtct
     7801 tctgacttga aaggaacaga gattggtggc gctttgattc cacgattgat tgatgaattg
     7861 cccattattg ccctgcttgc gactcaagcc caaggtgtaa cagttatcaa ggatgctgag
     7921 gagctcaagg tcaaggaaac agaccgtatt caggttgtgg cagacgcctt aaatagtatg
     7981 ggagcagata ttactcctac ggcagatggg atgattatca aaggaaaatc agctcttcac
     8041 ggtgctagag tcaatacgtt tggtgaccac cgtatcggca tgatgacagc tatcgcagcc
     8101 ctcttggttg cagatggaga ggtggagctt gaccgtgcag aagccatcaa taccagctat
     8161 cctagtttct ttgatgattt ggagagcttg attcatggct aaggtgttat tagggtttat
     8221 gggggctgga aaatcgacta ttgcaagagg cttggacact aattaccttg atatggatgc
     8281 tctgattgag aagcgcctag gtatgtccat tgcgaatttt ttcgctgaaa agggagaagc
     8341 agcctttcgt catgtagaat cagaagtcct agctgattta ctacaaacag accaagtcgt
     8401 gtcaactgga ggaggagtgg ttatttctca gagaaatcgt gacttactca agactaatac
     8461 agataacatc tacctgaaag cagattttga aaccctctac ctacgtatcg cagctgataa
     8521 ggacaatcag cgaccgcttt ttctaaataa tagcaaggaa gaactagtag ctatttttca
     8581 agaaagacag gcttggtatg aggaagtggc tagtcgggtt ttggatgtga ccaagctaag
     8641 cccagaggaa attatagagg aactaagatg aaaattgctt atctaggtcc caagggatca
     8701 ttttcacacc acgttgtgca gacagctttt cctcatgagg aattgcaggc ttttgacaac
     8761 attacagatg tcatcaaggc ttatgagcaa ggattagtgg actattctgt ggtgccagtt
     8821 gaaaactcta ttgagggtag tgttcatgaa accttggact atctttttca tcaggctcac
     8881 atccaagcag tagcagaaat cgttcagcct attcatcagc agttgatggt ggttccaggc
     8941 catactaaga ttgaaaagat tttttcacat ccacaggcct tggctcaagg aaagaaattc
     9001 atcgatgaac aatatccaga ggctcaaatc gaggtaacag ctagtacagc ttatgcggcc
     9061 cgttttattt ccgaacatcc agatcagccc tttgcagcaa ttgcacctag aagttctgct
     9121 gaagaatatg gattggaact gattgccgag gatattcagg aaatggaagc caatttcaca
     9181 cgtttctggg ttctaggagc tgaaaagcct agtattccct tgcaagcaca aactgaaaag
     9241 atgagtttgg ccttgacatt acctgacaac cttccaggtg cactttataa ggccctgtcg
     9301 acctttgctt ggcgaggaat tgacttgaca aaaattgaaa gtcgtccact caagacagca
     9361 ctgggtgaat actttttcat tatcgatgtg gattataccg ataaggactt ggtccacttt
     9421 gcccaaaaag aattagaagc gattggaatc cagtataaaa ttctgggtgc ctatcctatt
     9481 tatccaatat cagaccatgg aaaggagaga agatgagtaa agaaaatccc ttaagtcatc
     9541 atgagcagtt gcgttatgat tatttgctaa aaaatattca ctatctcaat gagagagaaa
     9601 aaaatgagtt tgcctatttg caagaaaagc taactcttgc taggggaaat agtagctcta
     9661 gcttggaaca agaaagagaa gagcaggttg acttaccaag ctatgcgaac cggagtcgct
     9721 cacaatccaa atcacaagca ctctctttcc ctccaaaaaa gaaaagacgg aagctccgtc
     9781 ttaaacgaat ttttatggtg attttttcac ttttggtctg tgtggctttg gccatggtat
     9841 tcatgttttt gcgtggttac caagatgcta gcgcaaagaa aactgctgat gcccgggcag
     9901 ctcaagtaga agtctttaat ggtcaggaca ctagagatgg agttaatatt ttaatcatgg
     9961 gtactgatgg tcgaatcggc cagaacagtg ttgagacacg aactgactct attatggtat
    10021 taaatgtcgg gggctcagat aagaaaatga agctagtcag tttcatgcgt gataatttgg
    10081 tctatataga tggttatagt caagtgatta acggtagaaa acagacagat aacaagttaa
    10141 acgtagccta cgagttagga gaacaagagg ggcaaaaagg ggcagaaatg gttcgccaag
    10201 tcttgaaaga taattttgac ttggacatta agtactatgc cttggttgat ttccaagcct
    10261 ttgccacagc gattgacacg cttttccctg atggggtgac gattgatgct caattttcaa
    10321 cattgaatgg gcgtccccta acagaagcta cagtcggaga tgatttatac gctaccgaga
    10381 ctgagtctcc aacccaaacc attaaagtcg gaaaacagca gatgaatggc tcgaccctgc
    10441 tcaattatgc tcgtttccgt gatgatgatg aggcggatta cggccgtaca aaaagacagc
    10501 aacaagtttt aacagcaatt ttagagcaaa ttaaagatcc cactaaactt ttcactggtt
    10561 ctgaagctct tggaaaggtt tttgctatga cctcaaccaa cgtaccctat actttcctcc
    10621 taacaaacgg tttatctgtt ttggatggag caaaaaatgg tattgaaaaa ttgacgattc
    10681 cagaactggg tgactgggta gatgcctatg atgtttatgg aggcttgggc ttgctggttg
    10741 atcaaaacaa atatcaaacc aagctcgctc aaatgggctt gagataagat aatactcaat
    10801 gaaaatcaaa gtgcaaacta ggaagctagc cgcaggctgc taaaaacaca gttttgaggt
    10861 tgtagataag actgacgaag tcagttacct atacctacgg caaggtgaag ctgacgtggt
    10921 ttgaagagat tttcgaagag tataagacag aaaaatcaaa gtttgatagc tatttggata
    10981 gcagtcaggc tttgattttt tacagtctgc aatagatttt caccccttgt ttgtggtata
    11041 atgaaacgat acgatagaaa gaataatgaa caatatgact gatttaaaag caattcaggc
    11101 tcgtagtctg gagatggctg aatattttgt ggccttttgt aaagaacatg atttgctctg
    11161 ttatctctgt ggcggaggcg ctattggtac ccttcgaaac aagggattta ttccttggga
    11221 cgacgaccta gactttttta tgcctcgtaa agattatgag aaattagcag aattatggcc
    11281 tcgttatgca gatgaacgtt atttcttgtc aaagagtcac aaggattttg ttgatcgtaa
    11341 tctttttatt accattcgtg acaagaaaac cacctgtatc aagccttatc agcaggattt
    11401 ggatttgcca catggtctgg ccttggatgt tttgcctttg gattattatc cgaaaaatcc
    11461 agctgagcgg aaaaaacagg ttcgttgggc cttgatttat tcactctttt gtgcgcaaac
    11521 tattccagaa aagcatggtg ctctcatgaa atggggaagt cgcattttac tgggtttgac
    11581 tccaaaatct ctccgttatc gcatctggaa aaaagctgag aaagaaatga ctaagtatga
    11641 tttggctgat tgtgatggca ttacagaatt atgctcaggt cctggctaca tgagaaacaa
    11701 gtacccaatc acatcttttg aagacaatct tttcttgcca tttgaaggaa cagagatgcc
    11761 tattccaatc ggctatgatg tctatctcag aactgctttt ggggattata tgacgcctcc
    11821 accagcagac aagcaggtac cgcatcatga tactgtcatt gctgatatgg ataagtctta
    11881 tacagaatac aagggagaat atggtggcta agaaaaaaat cttatttttt atgtggtctt
    11941 tttctcttgg aggtggtgca gagaagattc tatcaaccat tgtttcaaat ctggatccag
    12001 aaaagtatga tattgatatt cttgaaatgg agcactttga caagggatat gaatctgttc
    12061 caaagcatgt acgcatttta aaatcccttc aagattatcg ccaaaccaga tggttacgag
    12121 cttttttgtg gagaatgaga atttattttc caagactggc tcgtcgtttg cttgtaaagg
    12181 atgattatga tgttgaagtt tcttttacca ttatgaatcc accactgttg ttctctaaaa
    12241 gaagagaagt caagaagata tcttggattc atggaagtat tgaagaactt cttaaggata
    12301 gctctaaaag agaatcacat agaagccagt tggatgctgc gaatacaatt gtagggattt
    12361 caaaaaagac cagcaattct atcaaggaag tttatccaga ttatacttct aaattacaga
    12421 caatctacaa tggatatgat tttcagacta ttctagaaaa atctcaagag aagatcgata
    12481 tcgagattgc tcctcaaagt atctgtacta tcggacggat tgaggaaaat aagggttctg
    12541 accgtgtagt ggaagtgata cgattattac accaagaggg aaaaaactat catctctatt
    12601 ttatcggggt tggtgatatg gaagaggaac tgaaaaaacg agtcaaagag tatgagattg
    12661 aggactatgt acatttcctt ggttatcaaa aaaatcctta tcagtattta tctcagacga
    12721 aagttctctt gtctatgtct aaacaagaag gttttcctgg agtgtatgtg gaggccttga
    12781 gtctgggact cccttttatc tctacggacg ttggaggggc tgaggaatta tcccaagaag
    12841 gacgatttgg acaaatcatt gagagcaatc aagaggcagc tcaggcgatt actaattaca
    12901 tgacttctgc ctcaaacttt gatgtcgatg aggctagcca attcattcaa caatttacaa
    12961 ttacaaaaca aatcgaacaa gtagaaaaac tattagagga gtagcatgga aactgcatta
    13021 attagtgtga ttgtgccagt ctataatgtg gcgcagtacc tagaaaaatc gatagcttcc
    13081 attcagaagc agacctatca aaatctggaa attattcttg ttgatgatgg tgcaacagat
    13141 gaaagtggtc gcttgtgtga ttcaatcgct gaacaagatg acagggtgtc agtgcttcat
    13201 aaaaagaacg aaggattgtc gcaagcacga aatgatggga tgaagcaggc tcacggggat
    13261 tatctgattt ttattgactc agatgattat atccatccag aaatgattca gagcttatat
    13321 gagcaattag ttcaagaaga tgcggatgtt tcgagctgtg gtgtcatgaa tgtctatgct
    13381 aatgatgaaa gcccacagtc agccaatcag gatgactatt ttgtctgtga ttctcaaaca
    13441 tttctaaagg aatacctcat aggtgaaaaa atacctggga cgatttgcaa taagctaatc
    13501 aagagacaga ttgcaactgc cctatccttt cctaaggagt tgatttacga agatgcctat
    13561 taccattttg atttaatcaa gttggccaag aagtatgtgg ttaatactaa accctattat
    13621 tactatttcc atagagggga tagtattaca accaaaccct atgcagagaa ggatttagcc
    13681 tatattgata tctaccaaaa gttttataat gaagttgtga aaaactatcc tgacttgaaa
    13741 gaggtcgctt ttttcagatt ggcctatgcc cacttcttta ttctggataa gatgttgcta
    13801 gatgatcagt ataaacagtt tgaagcctat tctcagattc atcgtttttt aaaaggccat
    13861 gcctttgcta tttctaggaa tccaattttc cgtaagggga gaagaattag tgctttggcc
    13921 ctattcataa atatttcctt atatcgattc ttattactta aaaatattga aaaatctaaa
    13981 aaattacatt agaacggggg tgaccaagtg attgatggga aacgattatt atttagtttg
    14041 accatagtca gttatgcctt gacgctagta agtggaattg tgtatctgtt taataataat
    14101 aatgttagct tactttctac tttattgttc ttgttggtta gtagcttaat tgcttgttgg
    14161 aatgatatca agtattactt aatccatttt attttctatt taaccatttt tgtatttctg
    14221 gtatcaagac cgaccattga ttattttagg gatggtgctt tggataccta tcatccaata
    14281 gcctatcgtt ttgcctttat agttgtcatg gtttcgattc tgggcttgac cacaggaggc
    14341 gtcctgactc gttacttcat agctaggaag aaaataaaag tagcaaatat aggaaattct
    14401 ctaaaagagg tttatatcaa gcggttacgc tttgtatcac taggagtttt tcttctaacc
    14461 tatcctttct atttcattcg gttatttgaa cggctcttgt atcgtttgca gacttcctac
    14521 tatgcctact atgcaaattt tgaaagtaaa ctgccttatt ttacctacat tttgtctacc
    14581 tttacggtct atgcaatgtg tatgtatctg gcaaccaagc caaagaaatt gcaggccaca
    14641 gcagtgcttg tctcctttat tgcagctaat actattcatt tggcaattgg gacacgaaat
    14701 ccctttattt taagtatttt atttgctttt gtttattatt ttatgcggga gcaaactgaa
    14761 aaaggaaaat ggattgggtt taaagaaaag ttagcgattt ttgtaggttc tcctattctc
    14821 atgttagcga tgggagtact caattatgta cgggataatg tccaagtttc ccatacaggt
    14881 ttctgggata tcttacttga ctttatctat aaacaaggga ctagttttgg tgttctggct
    14941 cgaggttttc tatttaacag tagcctccct taccgagatt tccgtaattt tacttttggt
    15001 cctgttcttg attattttgc aagggggagt ttgggagcca ttttcggagg aaaagccttt
    15061 gaacatacaa ccaatagtgt ggaactagct attgatagta atagttatgc ccacaatcta
    15121 tcctatcttg tcttgaacaa ggaatacttg aaagggcatg gtatcggaag tagttatatc
    15181 atggagttgt ataccgacta tggtatgatt ggagtctttc tgcttagttt cttactcggc
    15241 gtattattta tagccatgct gcaagtagcc tatcgctcaa ggacaatcct atttgcttta
    15301 tccctactca tcttgaataa tctattcttt atgccaagaa gcagcttttc agaaagtttc
    15361 ttcaatttat ttacaatgca attctgggga attgttcttg tgattatatt tgtagcaaaa
    15421 atgcttacaa aggaaaacta gtatctacta aacaaaggag aaaaaaatca tgtttgaaca
    15481 ttattcagta gctgatttgt ttgcaaatct ttataaaaaa cgaaaagcaa atattttagc
    15541 tctcatcgct ttatttgctc tcattgctgt accatttaca attaaagcag ttaggaataa
    15601 aaacactgtc aaagatacaa caagttattc aacttatctt atctataaaa tcactcctcc
    15661 agaagattcg gccaaaacga ttttgaatca tcaaattggt ggttatagtg atttttatgg
    15721 gaaattgatt gatggtaatt tgaatggagc ttatcttttc aatgatgtag aacccagtga
    15781 gttgaaaaaa attgccagtg aattagacac gacggaaaca accttgaaaa attcaacgaa
    15841 tgactattgg tggaaaaaat tgaccgtcta ctatatgatt gacgatgcag gggttggtgt
    15901 gaaaattttg acatcaagta aagatgccaa tgacttgtta gagaaaaaaa ttgatgggtt
    15961 gattgagaaa tttaaacatg cttatgcaaa tgtgaaaatt gaaaaactgg aaaccatcaa
    16021 ctctaaagaa ttgaacgcaa atggtgaaac agcgcttggc ttaaatgtga aaaatctgat
    16081 tcttcgttta gttgttattg gagtggtttg tgtgattttg gttgtgatgg gaaatgtgtt
    16141 agtttatctc tttaatccaa caatcaatag agtaggtgat ttttctcagt atcaaattga
    16201 ttttgtaaca gagattacaa caattgctaa cctagcagat gttttgtcat acaaaaatac
    16261 tggacaggaa ttgaccatcg ttagctcaaa taaagctatc ctagataaat tgaaacagag
    16321 tcaagaagct ttaaaaggaa tgcattttgt agatttgcag gatgtatcat ctcttttgga
    16381 aagagataca gtccttcttg ttgaagagta cggagtgact cgttataaga aatttgagca
    16441 aagtcttcaa attctcagaa acttaaatcg ttctatcctc ggtgtagcaa cctttaaatt
    16501 ataagtattc tgatgtataa ggtcttcaaa atcggctaag agccgatttt gaaggctttt
    16561 tctatctgtc tgcagaaatt ttcttttgga gtctcctata gaaagaccct agggaaaatc
    16621 tatccgtata gaggagcctt cttttgataa aatcgtaaaa atctagtata atagatagaa
    16681 ctgaaagtat gaggttacta gcaatgaaaa tgaaacaaat tagtgataca actttaaaaa
    16741 tcacgatgtc tttagaggat ttgatggatc gtggaatgga gattgctgac tttctcgttc
    16801 ctcaagaaaa aacagaagag ttcttttatg ctatcttgga tgagctagag atgcctgata
    16861 gctttctaga tacaggcatg ttgagcttcc gtgtgactcc aaaacctgat aaggtagatg
    16921 tctttgtgac caagtcaaag attgaccaaa atctagattt tgaagactta tcggatttgc
    16981 cagatatgga agaattggct caaatgtctc cagatgaatt tatcaaaacc ctggaaaaaa
    17041 gcatagcaga caaaaccaag gatgatatcg aagccattca atctcttgag caagttgaag
    17101 ccaaggaaga agagcaagag caggctgaac aagaagctga gagtaagaaa gaaccttaca
    17161 tctactacat cctttctttt gctaagttgg ctgacttggt agcttttgcc aagacagtga
    17221 cttttgaaat ggaaacttct gaactctaca aaatgaacga gcgctattat ttgaccattt
    17281 tagtggatat tgaaaatcat ccaagcccat atccagcttg gctgttggcc cgtatgcgcg
    17341 agtttgcaga cgatagtgat atcagtcgct cagtcttaca agagtatggt caagtcttga
    17401 tgagtcacga tgcagtgctc aatctgcaaa aaatcggcta atcttttctg gaaagctatt
    17461 ttcagatttt aagaagagcg aatcgtctga ttcgtttttt ctttttatac tgaaatagtg
    17521 atttactata ataggaattt tcacaaaatt ctgttataat ggctatatta gaaaatttcg
    17581 aggagataaa gatgacagtt aaaattgctt tactaggatt tggtaccgtt gcaagtggtg
    17641 tgcctttcct cctaaaggaa aatggaggaa aaatcaatca atcagcacat tcagatatca
    17701 aagttgctaa ggtattggtc aaggatgaag atgaaaaaaa tcacttgctt gcagcaggga
    17761 atgactttaa ctttgtaacc aatgtggatg atattttatc agaccaggat attactatcg
    17821 tagtggaatt gatggggcgt attgagcctg ctaaaacctt tatcactcgt gccttggaag
    17881 ctggaaaaca cgttgttact gctaacaagg accttttagc tgtccatggc gcagaattgc
    17941 tagaaatcgc tcaagctaac aaggtagcac tttactacga agcagcagtt gctggtggga
    18001 ttccaattct tcgtacttta gcaaattcct tggcttctga taaaattacg cgcgtgcttg
    18061 gagtagtcaa cggaacttcc aacttcatgg tgaccaagat ggtggaagaa ggctggtctt
    18121 acgatgatgc tcttgcggaa gcacaacgtc taggatttgc agaaagcgat ccgacgaatg
    18181 acgtagatgg gattgatgca gcctacaaga tggttatttt gagccaattt gcctttggca
    18241 tgaagattgc ctttgatgat gtagcccaca agggaatccg caatatcaca ccagaagacg
    18301 tagctgtagc tcaagagctt ggttacgtag tgaaattggt tggttctatt gaggaaactt
    18361 cttcaggtat tgctgcagaa gtgactccaa ccttcctacc taaagcgcac ccacttgcta
    18421 gtgtgaatgg cgtaatgaac gctgtctttg tagaatctat cggtattggt gagtctatgt
    18481 actacggacc aggtgcgggt caaaaaccaa ctgcaacaag tgttgtagct gatattgtcc
    18541 gtatcgttcg tcgtttgaat gatggtacca ttggcaaaga cttcaacgaa tatagccgtg
    18601 acttggtctt ggcaaatcct gaagatgtca aagcaaacta ctatttctca atcttggctc
    18661 cagactcaaa aggtcaggtc ttgaagttgg ctgaaatctt caatgctcaa gatatttcct
    18721 ttaagcaaat ccttcaagat ggcaaagagg gtgacaaggc gcatgtcgtt atcatcacac
    18781 acaagattaa taaagcccag cttgaaaatg tctcagctga attgaagaag gtttcagaat
    18841 tcgacctctt gaataccttc aaggtgctag gagaataaga tgaagattat tgtacctgca
    18901 accagtgcca atatcgggcc aggttttgac tcggtcggtg tagctgtaac caagtatctt
    18961 caaattgagg tctgcgaaga acgagatgag tggctgattg aacaccagat tggcaaatgg
    19021 attccacatg atgagcgtaa tctcttgctc aaaatcgctt tacaaattgt accagacttg
    19081 caaccaagac gcttgaaaat gaccagtgat gtccctttgg cgcgcggttt gggttcttcc
    19141 agctcggtta ttgttgctgg gattgaacta gccaaccaac tgggtcaact caacttatca
    19201 gaccatgaaa aattgcagtt agcgaccaag attgaagggc atcctgacaa tgtggctcca
    19261 gccatttatg gtaatctcgt tattgcaagt tctgttgaag ggcaagtctc tgctatcgta
    19321 gcagactttc cagagtgtga ttttctagct tacattccaa actatgaatt acgtactcgc
    19381 gacagccgta gtgtcttgcc taaaaaattg tcttataagg aagctgttgc tgcaagttct
    19441 atcgccaatg tagcggttgc tgccttgttg gcaggagaca tggtgaccgc tgggcaagca
    19501 atcgagggag acctcttcca tgagcgctat cgtcaggact tggtaagaga atttgcgatg
    19561 attaagcaag tgaccaaaga aaatggggcc tatgcaacct acctttctgg tgctgggccg
    19621 acagttatgg ttctggcttc tcatgacaag atgccaacaa ttaaggcaga attggaaaag
    19681 caacctttca aaggaaaact gcatgacttg agagttgata cccaaggtgt ccgtgtagaa
    19741 gcaaaataaa gaatagaaga taggatgggg aaactcttga ccagagggtt tcatatcctt
    19801 tttgtgaaaa gaagtttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
    19861 ggctgctcaa aacagtgttt tgaggttgtg gatagaactg acgaagtcag taacatatat
    19921 acggtaaggt gacgctgacg tggtttgaag agattttcga agagtattag ttaaaaacgt
    19981 gataaaggag aaataaagat ggcagaaatt tatctagcag gtggttgttt ttggggccta
    20041 gaggaatatt tttcacgcat ttctggagtg ctagaaacca gtgttggcta cgctaatggt
    20101 caagtcgaaa cgaccaatta ccagttgatc aaggaaacag accatgcaga aacggtccaa
    20161 gtgatttacg atgagaagga agtgtcactc agagagattt tactttatta tttccgagtt
    20221 atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg gtcgccaata tcgaactggg
    20281 atttattatc aggatgaagc agatttgcca gctatctaca cagtggtaca ggagcaggaa
    20341 cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat tacgccacta cattctggct
    20401 gaagactacc accaagatta tctcaggaag aatccttcag gttactgtca tatcgatgtg
    20461 accgatgctg ataagccatt gattgatgca gcaaactatg aaaagcctag tcaagaggtg
    20521 ttgaaggcca gtctatctga agagtcttat cgtgtcacac aagaagctgc tacagaggct
    20581 ccatttacca atgcctatga ccaaaccttt gaagagggga tttatgtaga tattacgaca
    20641 ggtgagccac tcttttttgc caaggataag tttgcttcag gttgtggttg gccaagtttt
    20701 agccgtccga tttccaaaga gttgattcat tattacaagg atctgagcca tggaatggag
    20761 cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg gtcatgtttt cacagatgga
    20821 ccgcgggagt taggcggcct ccgttactgt atcaattctg cttctttacg ctttgtggcc
    20881 aaggatgaga tggaaaaagc aggatatggc tatctattgc cttacttaaa caaataaaac
    20941 agagagtggg gcttcccact ttcttcattt ctagaatatg aatagaaggg atttatgaaa
    21001 cacctattat cttacttcaa accctacatc aaggaatcaa ttttagcccc cttgttcaag
    21061 ctgttagaag ctgtttttga gctcttggtt cccatggtga ttgctgggat tgttgaccaa
    21121 tctttacctc agggagatca aggtcatctc tggatgcaga ttggcctgct ccttatcttt
    21181 gcagtaattg gcgttttagt ggccttgata gctcaatttt actcagcaaa ggcagcagta
    21241 ggttttgcta aggaattgac aaacgatctt tatcgtcata ttctttcctt gcccaaggac
    21301 agcagagacc gtctgacaac ttctagtttg gttactcgct tgacttcgga tacctaccag
    21361 attcagactg gtatcaatca attcctgcgt ctctttttac gagcgcccat tatcgttttt
    21421 ggtgccattt ttatggctta tcgaatctca gctgagttga ctttctggtt cttagtcatg
    21481 gttgccattt tgaccattgt cattgtaggg ttatctcgat tggtcaatcc tctctacagt
    21541 agtctcagaa agaaaacgga ccaactggtt caggaaacgc gccagcaatt gcaagggatg
    21601 cgggttattc gtgcttttgg tcaagaaaaa cgagagttac agatttttca aacccttaac
    21661 caagtttatg ctagattaca agaaaagaca ggtttcttgt ctagtttatt aatacctctg
    21721 acctatctga ttgtcaatgg aactcttctc gttattatct ggcaaggcta tatttcaatt
    21781 caaggaggag tactcagtca aggtgctctc attgctctta tcaattacct cttacagatt
    21841 ttggtggaat tggtcaagct agccatgctg atcaattccc tcaaccagtc ctatatctca
    21901 gccaagagaa tcgaggaggt ctttgcggaa tctcccgaaa acatccattc agaattagaa
    21961 caaaagcaag ttaccagtgg tcgggtttta caagtccaag aattgacttt tacctatcct
    22021 gatgcggccc agccttctct gagagacatt tcctttgata tgactcaagg acaaatcctt
    22081 ggtatcattg gggggactgg ttctggtaaa tcaagcttgg tgcaactctt acttggactt
    22141 tatccagtag acaaggggaa cattgacctt tatcaaaatg gacgtagtcc tcttaatttg
    22201 gagcagtggc ggtcttggat tgcctatgta cctcaaaagg tcaaactctt taagggaact
    22261 attcgttcca acttgacttt aggtttaaat caagaagtat ctgaccagaa actctggcag
    22321 gccttggaga ttgcgcaagc taaggatttt gtcagtgaaa aggaaggact tttggatgcc
    22381 ctagttgagg caggggggcg aaatttctca ggtggacaaa aacaaaggtt gtctatcgcc
    22441 cgagcagtct tgcgccaagc tccgtttatc atcctagatg atgcaacctc ggcactggat
    22501 accattacag agtccaagct cttgaaagct attagagaaa attttccaaa cacgagctta
    22561 attttgatct ctcaacgaac ctcaacttta cagatggcgg accagattct cctcttggaa
    22621 aaaggtgagt tgctagctgt tggcaagcac gatgacttga tgaaatccag ccaagtctat
    22681 cgtgaaatca atgcatccca acatggaaag gaggactaga atgaaacgac aaactgtaaa
    22741 ccagacgctc aaacgtttag ccgtagattt agcaaaccat tccttcctcc ttttcctagc
    22801 ctttctagga actattgccc aagttggctt atcaatttac ctacctattc tgattgggca
    22861 ggtcattgac caagtcctag tggctggttc atcaccagtt ttttggcaga tttttctcca
    22921 gatgctcttg gtggtaatag gaaatactct ggtacaatgg gccaatcctc tcctctataa
    22981 tcgtctaatc ttctcttata ccagagattt gcgagagcga atcatccata agctccatcg
    23041 tttaccgatt gcttttgtag ataggcaagg tagtggagag atggttagtc gtgtaaccac
    23101 ggacatcgaa cagttggcag ctggcttgac catgattttt aaccaatttt tcattggtgt
    23161 tttgatgatt ctggttagta ttctagccat gctccaaatt catctcctca tgactctctt
    23221 agtcttgctg ttgacgccac tgtccatggt gatttcacgc tttattgcca agagatccta
    23281 tcatctcttc cagaagcaaa cagagacgag gggaattcag actcagttga ttgaagaatc
    23341 gcttagccag cagactataa tccagtcctt caatgctcaa acagaattta tccaaagatt
    23401 gcgtgaggct catgacaact actcaggcta ttctcagtca gccatctttt attcttcaac
    23461 ggtcaatcct tcgactcgct ttgtaaatgc actcatttat gcccttttag ctggagtagg
    23521 agcttatcgt atcatgatgg gttcaacctt gaccgtcggt cgtttagtga cttttttgaa
    23581 ctatgttcag caatacacca agccctttaa tgatatttct tcagtgctag ctgagttgca
    23641 aagtgctctg gcttgcgtag agcgtatcta tggagtctta gatagccctg aagtggctga
    23701 aacaggtaag gaagtcttga ccagtgacca agttaaggga gctatttcct ttaaacatgt
    23761 ctcttttggc taccatcctg aaaaaatttt gattaaggac ttgtctatcg atattccagc
    23821 tggtagtaag atagccatcg ttggtccgac aggtgctgga aaatcaactc ttatcaatct
    23881 ccttatgcgt ttttatccca ttagctcggg agatatcttg ctggatgggc aatccattta
    23941 tgattataca cgagcatcat tgagacagca gtttggtatg gtgcttcaag aaacctggct
    24001 cacacaaggg accattcatg ataatattgc ctttggcaat cctgaagcca gtcgagagca
    24061 agtaattgct gctgccaaag cagctaatgc agactttttc atccaacagt ttccacaggg
    24121 atacgatacc aagttggaaa atgctggaga atctctctct gtcggccaag ctcagctctt
    24181 gaccatagcc cgagtctttc tggctattcc aaagattctt atcttagacg aggcaacttc
    24241 ttccattgat acacggacag aagtgctggt acaggatgcc tttgcaaaac tcatgaaggg
    24301 ccgcacaagc ttcatcattg ctcaccgttt gtcaaccatt caggatgcgg atttaattct
    24361 tgtcttagta gatggtgata ttgttgaata tggtaaccat caagaactca tggatagaaa
    24421 gggtaagtat taccaaatgc aaaaagctgc ggcttttagt tctgaataag ccattctctt
    24481 ttgaaagttt atggacgaaa aaagttgcct tcgagtgact tttttgttac aatagctaga
    24541 aaaattgttc actgtaatac tcaatgaaaa tcaaagagca aactaggaag ctagccgtag
    24601 gttgctcaaa acagtgtttt gaggttgcag atagaactga cgaagtcagt aaccacacct
    24661 acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta gaaaaggagc
    24721 ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaagat ttccatgttt
    24781 atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt caagataagc
    24841 cagcgttttt agagcaagct gagcagatta tggactatca gggagcttgg attatgcctg
    24901 gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc cgagatgaca
    24961 gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag tttactcccg
    25021 acatgactac caatgcggtc aaagaagccc tgacagagat gctccagtca ggaacaacaa
    25081 cctttaacga tatgtataat cccaatggtg tggatatcca gcaaatttat caggtggtga
    25141 aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag acagaaacaa
    25201 ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa tataaaaatc
    25261 caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga gacttgctgg
    25321 aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat gtggcggaga
    25381 ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt gcttttcttg
    25441 aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa ttaaacgaga
    25501 gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct atcagtaacc
    25561 tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga gtagcagtcg
    25621 gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag gaaggaagga
    25681 ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca atcgaaacag
    25741 ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat cagataggaa
    25801 gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg aaaattcatc
    25861 tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct aatgatgtag
    25921 atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg acagtagaac
    25981 tttaaaagaa aaat
//
LOCUS       DAVGJJ010000022        25168 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000022 DAVGJJ010000000
VERSION     DAVGJJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.022"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1127
                     /locus_tag="VVE35_000815"
                     /pseudo
     CDS             1..1127
                     /locus_tag="VVE35_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1202..2131)
                     /locus_tag="VVE35_000816"
     CDS             complement(1202..2131)
                     /locus_tag="VVE35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU3061044.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            2271..2558
                     /locus_tag="VVE35_000817"
     CDS             2271..2558
                     /locus_tag="VVE35_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU3061045.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(2592..3926)
                     /gene="ciaH"
                     /locus_tag="VVE35_000818"
     CDS             complement(2592..3926)
                     /gene="ciaH"
                     /locus_tag="VVE35_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU3061046.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(3916..4590)
                     /gene="ciaR"
                     /locus_tag="VVE35_000819"
     CDS             complement(3916..4590)
                     /gene="ciaR"
                     /locus_tag="VVE35_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU3061047.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(4699..7245)
                     /locus_tag="VVE35_000820"
     CDS             complement(4699..7245)
                     /locus_tag="VVE35_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU3061048.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(7368..7808)
                     /locus_tag="VVE35_000821"
     CDS             complement(7368..7808)
                     /locus_tag="VVE35_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU3061049.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(7783..8652)
                     /locus_tag="VVE35_000822"
     CDS             complement(7783..8652)
                     /locus_tag="VVE35_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU3061050.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(8630..9130)
                     /locus_tag="VVE35_000823"
                     /pseudo
     CDS             complement(8630..9130)
                     /locus_tag="VVE35_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            complement(9141..9839)
                     /locus_tag="VVE35_000824"
     CDS             complement(9141..9839)
                     /locus_tag="VVE35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU3061051.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLRRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQEIQEIFEINYMTPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(9832..10068)
                     /locus_tag="VVE35_000825"
     CDS             complement(9832..10068)
                     /locus_tag="VVE35_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU3061052.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(10168..11094)
                     /locus_tag="VVE35_000826"
     CDS             complement(10168..11094)
                     /locus_tag="VVE35_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU3061053.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(11169..11984)
                     /locus_tag="VVE35_000827"
     CDS             complement(11169..11984)
                     /locus_tag="VVE35_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061054.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVICIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(11988..12308)
                     /locus_tag="VVE35_000828"
     CDS             complement(11988..12308)
                     /locus_tag="VVE35_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU3061055.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(12605..12685)
                     /locus_tag="VVE35_000829"
                     /pseudo
     CDS             complement(12605..12685)
                     /locus_tag="VVE35_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(12729..14726)
                     /gene="metG"
                     /locus_tag="VVE35_000830"
     CDS             complement(12729..14726)
                     /gene="metG"
                     /locus_tag="VVE35_000830"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU3061056.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEERQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(14850..16109)
                     /locus_tag="VVE35_000831"
     CDS             complement(14850..16109)
                     /locus_tag="VVE35_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061057.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(16111..16812)
                     /locus_tag="VVE35_000832"
     CDS             complement(16111..16812)
                     /locus_tag="VVE35_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061058.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(16796..17995)
                     /locus_tag="VVE35_000833"
     CDS             complement(16796..17995)
                     /locus_tag="VVE35_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU3061059.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            18281..19627
                     /gene="gor"
                     /locus_tag="VVE35_000834"
     CDS             18281..19627
                     /gene="gor"
                     /locus_tag="VVE35_000834"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU3061060.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRHDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFTSMYSACTCNRQETRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            19824..20360
                     /locus_tag="VVE35_000835"
     CDS             19824..20360
                     /locus_tag="VVE35_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU3061061.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            20416..20496
                     /locus_tag="VVE35_000836"
                     /pseudo
     CDS             20416..20496
                     /locus_tag="VVE35_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20565..20780)
                     /locus_tag="VVE35_000837"
     CDS             complement(20565..20780)
                     /locus_tag="VVE35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU3061062.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            20878..21198
                     /locus_tag="VVE35_000838"
     CDS             20878..21198
                     /locus_tag="VVE35_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061063.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(21229..21567)
                     /locus_tag="VVE35_000839"
     CDS             complement(21229..21567)
                     /locus_tag="VVE35_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061064.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(21579..22298)
                     /gene="trmD"
                     /locus_tag="VVE35_000840"
     CDS             complement(21579..22298)
                     /gene="trmD"
                     /locus_tag="VVE35_000840"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU3061065.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(22288..22806)
                     /gene="rimM"
                     /locus_tag="VVE35_000841"
     CDS             complement(22288..22806)
                     /gene="rimM"
                     /locus_tag="VVE35_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU3061066.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(22837..23625)
                     /locus_tag="VVE35_000842"
     CDS             complement(22837..23625)
                     /locus_tag="VVE35_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3061067.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     RATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(23729..23972)
                     /locus_tag="VVE35_000843"
                     /pseudo
     CDS             complement(23729..23972)
                     /locus_tag="VVE35_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(24202..24441)
                     /locus_tag="VVE35_000844"
     CDS             complement(24202..24441)
                     /locus_tag="VVE35_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU3061068.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(24461..24733)
                     /gene="rpsP"
                     /locus_tag="VVE35_000845"
     CDS             complement(24461..24733)
                     /gene="rpsP"
                     /locus_tag="VVE35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU3061069.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            24903..25112
                     /locus_tag="VVE35_000846"
     CDS             24903..25112
                     /locus_tag="VVE35_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061070.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
BASE COUNT         7427 a         5553 c         4465 g         7723 t
ORIGIN      
        1 taaaattcct tatcttgaaa cgactggtat gcctactaga atcctcctta gaaagcgtcg
       61 attcaagtgc tatcactgtt caaaaatgat ggtcgctgaa acttctatcg tcaagaagaa
      121 tcaccaaatc cctcgtatca tcaaccaaaa gattgctcaa aagttgattg aaaagatttc
      181 tatgactgat attgcccatc agctggccat ttcaacttca actgtcattc gcaagctcaa
      241 tgacttccac tttgagtgta attttagaaa tctgcctgag attatgtctt gggaggttga
      301 aacagtccgg ggagtgactg tttcaatcgg gagatggaga tgagtttcat tgcgcaagat
      361 tttgataatc ttaatattat cactgttctt gagggaagaa cacaagctat cattcgaaat
      421 cactttcttc gctacgatag agttgtccga tgtcgcgtca aaattattac tatggatatg
      481 tttagccctt actatgactt ggctaaacag cttcgctttc aaatttctag gctcaggctg
      541 aaacagtctc ccagactgtt tcactcccga atgctaaaat cgttcttgat cgctttcaca
      601 ttgtgcaaca tcttagccgt gctatgagtc gtgtgcgtgt tcaaatcatg aatcagtttc
      661 atcgaaaatc ccatgaatac aaggctatca agcgctactg gaaactcatt caacaggata
      721 gccgtaaact gagtgataag cgattttatc gtcctacttt tcgtatgtac ttaaccaata
      781 aagagatttt aaacaagctt ttgagctatt cagaagactt gaaacaccac tatcagctct
      841 ataataagga accagagaaa tttttcggac ttattgagga caatcttaag caggttcatc
      901 ctctttttca gactgtcttt aaaacctttc taaagaacaa agagaaaatc gtcaacgccc
      961 ttcaactacc ctattcaaac gccaaattgg aagcgaccaa taatctcatc aaacttatca
     1021 aacgcaatgc ctttggtttt cggaactttg aaaacttcaa aaaacggatt tttatcgctc
     1081 tgaacatcaa aaaagaaagg acgaaatttg tcctttctca accttagctg acttcaaccc
     1141 actacagttg acaaagagtc agaaatgttt gcatcttcct tctctcaacg aatcatgtat
     1201 tttatctaac catgtcagga tcgtagtcat aaaatcctgt atcaaggaaa cggtttttag
     1261 ggtgaagttt acgaacttct tcatccagca agaaggcttg atcatggcta aagttgccct
     1321 cttgaatcaa attacgcgct tggataaaga gttttgcaat ctcaacaaag ttctgaccag
     1381 gagtgtagag cccttctagt ttccagcgag taaagccatg ctccaccaat tctgttaatt
     1441 tgatcatcaa atcaaggtca ttgttggcaa agatatgggt cccatgatta tcttcaaaaa
     1501 tggaatagtg gctctctgga tcacttggct cagccaagaa gaggtcacgt ttatgcgtct
     1561 tttcatcatc gatatgtgta aagttatagt agttttgcaa gagtggacgt ttagaatgat
     1621 ggatgacgct agcaccgtaa accaaaactt cagcaggaat ttccaaaatc tctggcattt
     1681 tgaaaagttc agctgatgga atttcacgcg ccaaaacagc ctcagatgcg ccagcctttt
     1741 gcccccagaa gttaatctga cgactgctag ttaccatggt tgaagcatcg tagatggtct
     1801 taaatgaata accatcgcgg ttaactacgt aaaagacgcc tgcatcccca atcgtaatat
     1861 agtctgtctt gatttcttcc aagaagtcta agaaaggctt gatacggtcc atcatatctt
     1921 ggtgcatgag agcattgacc gcaacgagca attccttacc agcatcatga accaacttag
     1981 cgatttcacg taattggtca taactaaagg tcgttggcag acgaaggcca aaatctttct
     2041 caccgacata gatacggtct acgccagctt cgagtagttg ttcaacttgt tcaatacttt
     2101 cagcagttgc tgtaatgaca atcttttcca taagaaaaat tataccacat ttctccaaaa
     2161 tcagccatgc tttgaaaatg aaaagggact ttattctttt tgtaaccttt ctgtaataat
     2221 tctctgccga aaaaatgata aaataggtat gtactgttaa ggagagaatc atgcccgtaa
     2281 gaaaattaca atcctatgag gtagactatc aagaagagtt aaaccagcag cttcctcatt
     2341 accaagctta tacacctgaa gcgcaatctg atgccaatct caaggaaatc ctatttttta
     2401 ttaatatcgc tgttttttgt atctgtattg ctatctttag ttttatcttt ttagcattaa
     2461 aattatcaac tgctcttgcc tttgccgcag caatcggatt cagcttactt gttttaaaag
     2521 ttcaacggtc tattatcaaa cgaaaacgta gaagataaat ccaaaatatc gccccaattg
     2581 gagcgatatt tttatttttt ctttttagat ggtgtctgaa tggcaatctt cacttcaaag
     2641 attgttccct tgggtttatt atctttgaca gtaacagttc cttttagagc atctacaatt
     2701 tgcttggcta gggataatcc taaaccaaaa ccaccttttt gccgggttct agctttgtct
     2761 actcgataaa aacggtcaaa aattttcttt ttatcttctg tcgaaatacc gattccatta
     2821 tcagaaacaa gtaaataaag attgcgatcg gtcgccgaga taagaaaatc aatttcacca
     2881 tcctcctcag tatacttgac ggcattatcg aaaagaatgg tcatcagttg tttcagaaga
     2941 agctgatctg tgacaattgt tcgatggata cgattttcaa aacggaagac acgattattt
     3001 tccgaagcaa tcatctcgta gtttgtgaaa gttgtattaa aaaagctagt tggaacttct
     3061 gcaagctccg gcttaatccc atcatctctc cgagctaagt tcagcaagct tgtcgttaaa
     3121 aaacgcatat ttcggacttc ttccaaactc gatgcaatgc tttcgctcac atccataatg
     3181 gtagcttctg gcttacgaaa aagggtctct aagcgatttt gcaaaactgc gagtggagtt
     3241 cgtaactcat gactggcatt ttccacaaaa gactgttgct tctgcatact ctcaagcagg
     3301 ggcctaacac tgaccctagc tagatagaga ctggcaagta aagacaaaat ccagaaacta
     3361 gccatcacga ccacaatcaa ttgctcatgc ttttgactag cctgttccaa ctgactggta
     3421 ttaatcaaga cagcagcata cttgatattg gttgaaaccg aactaatatt ggtctccatc
     3481 aaaatcacac gatagatttc ttcctgccca tagctattaa aaacctgaat ctggtagata
     3541 tgtcctagtt ctttcttttc taacttaatc ttatccaagc ccaaaaatcg atttccagaa
     3601 agaagttgag taaagtcttt atcaaagaga atgacttccg tattggaact gacattaggt
     3661 tttatttcta ctttactagc gtccgcccta gcattttcta aatctttaat ctcttctgtt
     3721 gccctattta tagccagctg aataactgct tgaggatttt cactcagtcc atgaagctta
     3781 tcgtccaccg aagtataaag actcgaatgc atgacttgta aaataatcag agtcattgta
     3841 gaaaaaatca gggtgaagac accgaagttg cggataaaat aactaaagtc atccgcatac
     3901 catgtttttt taagtttact gaacatcttt taaaagatac ccaacactac gcaaggtttg
     3961 caaattctct gcaaaagtgg ttccctttaa tttcttacgg acttttgaaa catagacttc
     4021 gacaaccgaa atcgttgtat cactatcaaa tccccataga cggtcaaaaa tctgcgtctt
     4081 aggcaaaatc acattttgat tttgaaggaa ataaactagt aaatcgaact ctttccccag
     4141 caattcgaca ggagtatctt caactttaac ggtattggtt gataaattaa ccacgatatt
     4201 cccataagtc aaggtgtttt cattaaactt ccctgaacgt ttgagaaggg cctgaatccg
     4261 cattttaagt tcttctaggt agaaaggttt ggtcagataa tcatccgctc ccagttcaaa
     4321 tccatgtccc ttgtcatcca aactttcctt ggcagtcata atcagaactg gtgtcgtaat
     4381 tcccttttca cgcaattctt ttaagacttg gaaaccattt ttttctggca acatcaaatc
     4441 cagcaaaatc aagtcataga caccactctc agcttcgtag agaccttctt ctccatcaaa
     4501 tacctgcata acatccgcaa aatcgtctaa aaagtcaaat actgaatttg acagacctag
     4561 gtcatcctca accaataaga tttttatcat gtgaaactcc tccttattaa aactattata
     4621 ccaaatttgc cttaaaaaaa actcaactct ctgcatttta catgagatag ctgagttttc
     4681 tttttatttt aggcttattt atgcatttcc gtattgaaga acaactgctt cgactgcagc
     4741 tttttcacgg ctaatcaagt caacacgcgc tgcaatttcc ttgattccca taccgatgtt
     4801 acggctaaga gcaaggtcag aaagttgcgg ctcaaagaac tccttgtatt ccgccaagcg
     4861 ttgctgagtc ttaaatacat gagcaggaag gataacaaag ctatcaaagc tcatatctcc
     4921 tccaagggct gccttaatcc aagcccagtt ttcacgcgcc caagaccaag ctgttttctg
     4981 agttgcttga tgagctagga attggtaata ccaagcagac aagtcctgtg gtttgaccac
     5041 aaatttgtcc ttccaagaag taatcaagtt ttggatatta tccgcatctg tactgtatgc
     5101 aagagctgct gccaactggc gtttaaagac agcatctgtt gcgtgagtat aagtatcaag
     5161 ataaagtgct aacaagtctt tagtctcatg atgtttcatc tcattaatca gaacttgtga
     5221 gcgaatagct gctgggagtc ctgcaagatt ctccttgtgt gttgcgaaga tttggctagc
     5281 gacttgacta gcttctgcat catttgagcg aatcatcatc gaaacagcca gctgacgaac
     5341 caattcatcc tcatctgatt ctccgtcttt agcttcaaaa ccaagacggt catagttatg
     5401 acgagccaat ttagcaacca gtcctttgaa ggctgtttca gcatccgttc cttcatcaat
     5461 aaagcgctca agggctgaaa tcacttgaga aacagctgaa accaccagat aagactcttc
     5521 cttagcaagt ttatcaagaa ctggaagcaa gtctgcataa gaaatgtgcc ctgcctcagc
     5581 caacaaacga cgttcttgaa caatttgcag tttgcttgtg ttatcaagtg tctctagctc
     5641 agcaagaaca gcttctaaca agtctccttg atagtcggta atatagtggg cagtattttc
     5701 agtgttgaga cgaagagctc cttcattttc agcaagaaga gctgcgtagc cagggatttc
     5761 gatactttca gtttcgagtg tatcaggcaa gcctttccag ttgctattga ggggcaccac
     5821 ccagagacgg ttcttgtctt cgttctcacc gatgaagaat tgtttttgtg aaatcttcaa
     5881 gacatcattt tcaactttaa cagtaagaac tgggtaacca ggctgttcca accaagaatc
     5941 catgaaggct gcgacatcac gtcctgacgc ttgaccaagg gcatcccaaa ggtcactacc
     6001 aatggtgttg ctgtattggt gtttttcaaa gtaggcgtgc aaacctttag caaaatcagc
     6061 atctcctagc caacggcgaa gcatgtgcat gagacggctt cctttggcat agacgatagc
     6121 gccgtcaaag agtgtattga tttcatctgg atgtttaact tcgacgtgga cagactgaac
     6181 gccatcagta gcgtcacgtt caagagcaag aggtactcca cctgtttgga aatcttcaaa
     6241 gatattccag cttggttcga tggtatccac acagacgtat tccatcatat tagcgaaact
     6301 ttcattgagc caaaggtcat cccaccattt catagtcacg aggttcccaa accattggtg
     6361 agccaattca tgggccacaa caagggcaac ttgttgacgg ctagcaaatg tagagttctc
     6421 atcgacaacc aagtaaactt cacggtaggt cacaagaccc cagttttcca tagcaccagc
     6481 tgagaagtca ggaagggcga tgtggagaga ttgaggaatt ggatacttaa ctccatagta
     6541 atcttcgtaa aactcgatag agcgaacagc gatatccagt gagaaatcaa gatttgaaag
     6601 tggatgtgct ttggttgagt agacacctac cagggtacca tttttagttt tagcggtcac
     6661 cccttgcaaa tcaccagcaa caaaggccaa caagtaagaa gacatgcgag gtgttgtctc
     6721 aaacttccag atacctgttt ccttacggtt ttcaacatcg atttctggca tgtttgacaa
     6781 ggccaattca ccttctgctt ggtcaaagcg aagagagagg tcaaaagttg ctttggcttc
     6841 aggctcatcc acacatggga aagcttcgcg cgcaaaatgg ctctcgaact gagtagacaa
     6901 gacctccttc ttgactccat caactgtata ataagaaggg taaatccctg tcatgttgtc
     6961 tgtaatttta ccagaaaagg caagaaccaa ttcaacttga ccagcctcag ccaattcgat
     7021 atgaagggct tcattgtcat ggtcaactgt aaatggacga gcttgacctg caacttctac
     7081 agaggtgatt tccaagtctt tttggtggag ggagatgcgg tcactctgtg cttgaccagt
     7141 gatggtcact ttcccagaaa aagtcttggt ctcacgactc aaatctaaaa ataaatcata
     7201 atgttcagga acaaattgct taataaaatg ttcaactgct tgcatagttt tctcctattc
     7261 taagtttaag agctgctgct cttcttcaaa caaacttatt atatcatatt ttgcttgaaa
     7321 aaaaaggatt ggacagcgat ttccaaaact ttcttccttc taatagtcta cagtgggtag
     7381 accttgcgaa attcttctaa aacaaccttg ctttcagatg taaaagtcag ccctacttca
     7441 cccaaacagt ctgtgaaaaa gtcctcatga acctggcgcc aataggcaag tgatttgtct
     7501 ccctcacctt ccttaaaggc atgttgcgca gaaacttgat tgaagagttc aacagaaacc
     7561 tttgtaattt cgacaatgca gacagactga ttttgactat ctaaaatgat atcgaaggtc
     7621 cctacttggg gaagtggttc gtcttctagt acatagaggt cataggctga tgctgttgct
     7681 gtcttttctc ctttaaacac caaatccgct aaaaggtctg gttcaactcc aaaagcccaa
     7741 gcatcgattt catctccgat caaaggattg atttgcttgt atttattcca catttcttgc
     7801 ggtatcatgg gtactccttt gtaatttttt actttcttct tttatgtgtt taagatgatc
     7861 tggatggtca atctctaaat caaaaatctc tggaatagaa ctgtagtgga taatgcactt
     7921 gatacccaac tgattcattt tttgtatgaa agaagtattc agatagcctg ctacagcaaa
     7981 atcaatcttg ttctttcttg ctttatcctg catatctctt agcatatcta acattattgg
     8041 actttccata tcatgccatt gactgtttct catagtcgca aaaacaaagg aagtcaaatc
     8101 attcattcca actacaatct ttgaaatgcc cgtttccagt atactagata agtcaaaata
     8161 cgctgacggt aattcaatca tcgttccgac tttcccagta aaaccctgct gacgcaatac
     8221 tgtaatagct tgttttaatt ggtcgtcatc attgacaaaa ggaaagataa cagatagatt
     8281 ggggttggtt tgataaactt ctgtaacgac atgtgcttca gcctgaaatt catccaaaca
     8341 cgccagtaaa cgcctggttc ctctatagcc aaacaaggga tgcccttcgt caaaaaactc
     8401 tctagtcccc actaaacaat tggcttctgt attcgttaat tcagaaaaac gataccaaac
     8461 ttcttcatct gagtacaggg agcagatagt ctctagataa tcttttacaa attgctgaca
     8521 acttgctaat atgatgtttt gattaagctc tctcaacaaa tattccccac gaatcatgcc
     8581 gacgtggtga aatagttgag gataaatttt ttcaagaatt ttttcgccac taagggcaag
     8641 ttgatttctc atcattcacc ttccaattca tgtaagaagt cttgtccagt tctggaaatc
     8701 ctaataattc agacttaacc ttcaagacta atggcgatgc attttcttct gtaatctctt
     8761 gaatatccat ccaaatatat ccaagtgaat cattcgcacc atcagacaca gcttccgaag
     8821 tcgtaacttg aggtgcactc tcattcattt caacatcata caaggccatg acatggtgaa
     8881 ccataaaatt tgttaactct tctctgacga aaacatcgta gattcaagga ttagagtagc
     8941 ttctaacagt aaatcccgtc tcttccataa cttctctagt cagcgtttcc gtcagtcctt
     9001 caccaagttg ctgactgcct ctaggtagat cataccgatg ttgataaggg cctctcgttt
     9061 tttcaatgca gagtaacttc ccattttcta agcaaacagc gtagactcca aagtgttttt
     9121 tgatttccat ccaactcctc ctacttcaaa gaccagccac catctattgt caagatttgt
     9181 ccttgcatgg cgctcgcttt tccacttgct aaaaaaagac taagctctgc tatttcctct
     9241 ggctcaatcc agcgcttgat tggggtttca ctagccaccc agtcagccaa accacctggt
     9301 tcaaaatccg cagcggtcat agctgtcttg actgctcctg gagcgatacc aaagacctga
     9361 atcccagctt cagcatagtc tagagccaac tgcttggtga agccagccaa ggcatgcttg
     9421 gatgaagtat aggcgtgacc acctccacct gctaggctag aagcaatgga acacatattg
     9481 atgatgattc cctttttatt ttccagcatt tgtgtcaaat aataccgagt caactcaaca
     9541 ggagtcatgt agttaatttc aaaaatctct tgaatttcct gcgcagtctg ttccaacagt
     9601 ggtttgtaag catccaaaac tccggcagta ttacacaaaa catccacctg agggcaccag
     9661 tcaaaaatag gttccaagtc caaggtcaaa tctctccgta aaaagtgaaa atcaccctct
     9721 aagagtggct tttcaccttg gtcaactcca taaacttgat agcccttctc taaaaagagg
     9781 cgagcttgag ccaatccgat acctgaactc actcctgtaa taagtacacg tttagtcatg
     9841 cacttctacc caatccgttg ccaaaacatc acaagctgtc gggctccaca tggaaaaacc
     9901 ttctccttct ccagacacgt tgattaggaa ataaggtgtc acttcaagtg caagcccatt
     9961 ttgctcgatg gtatcaaaga gttggacata gttttccgca cctccccaac cagttcgtac
    10021 atattttttc ttagccttta agccaggtaa aatttcttca aatgtcatgt ttttctcctt
    10081 taatgtcaat aattctcccc ttcattataa caaaaaaacc gctttaaaac ggctttttga
    10141 ctgtgattta ttcaaacctg ctactactta cggcaaatta ttccctgcag caagataaat
    10201 ttcataccat tcttttcttg ttaagctaaa gtttgccgct cggctaactt ctctcaagtg
    10261 cttaggattt gttgtaccta cgactgcctg catttttgct ggataacgca atatccaaga
    10321 aatggcaata gttgaagagg ttactccata tttaatagct aaacgatcaa gtacttgatt
    10381 taaagcttga aatttctcat ttccaacaaa attcccttta aaatacccga attgcaagac
    10441 agaccatgct tgaatgacca catcgtgtaa tttgcaatat tcaaaaatgc tgccatctcg
    10501 catagctgct tggctatctt ccatattaac atgaaaagct gattcaaatc ctggagtaaa
    10561 agccgcactc aattgtagct gattaacagc taacggctgc ttgacatctt ttttaagcaa
    10621 ctccatcatc ataggatttt gattagaaac tccaaaatct cgaactttac cttgtttata
    10681 aaggagatca aaggcttctg ctacttggtc agattccatc aaagcatctg gtcgatgaag
    10741 gagcaagcta tctagatgat caatcttcaa tctttgcaaa ataccgtcta ctgattttat
    10801 aatatagtcc ttagaaaaat caaaataggt aaattcttca atgcgaatgc cacatttgga
    10861 ctgaatccac atcttttctc ttaaatctgg acgatttttt aggacaagac ctaacagttc
    10921 ttcacaacga ccacgaccat aaatatcagc caagtcgaag gcattgattc caacagaaag
    10981 tgctgtttct acaagctctt caacttcttt tacagactta tcttttattc tcatcattcc
    11041 gagaacaatt tctgataatt ctttgtcatc ttgaccaaga gttatgtatc tcatcaaatt
    11101 tttctccttt aatttctaac attcttccct tcattataac aaaaaaccgc tttgcaacgg
    11161 ctttttgact atacttcact ccattttatc ttcttaaacc cacggaacaa gacaaagatt
    11221 ccaataaaga ggacagctaa aggaataact tttgtaagga aaacatttga aattcccatc
    11281 cactcataat aacggagcag agaacccacc acaagatggg caataatcat actgacaaat
    11341 ggacgaaaga ccgcttcttt ccaattccaa atacagataa ctagcgaaat cgtaaagaca
    11401 gacaaactat cccagggagc cggaatataa aaggctcctt cttgtatgaa gcttgccatt
    11461 cctacatatc ctaaaacaac taaaagaact atagtcccaa caacaatgta agtgccaatt
    11521 ttcattttag gagaatcttg gactaaactt cttcgtaaaa ttgtggccac aagtccaaat
    11581 ccaatcagaa aaataagaag ttgccctaaa aatgtgagca aattgactgt taagagagga
    11641 cctttagaaa aatcacttag tagttgataa taacgtaata ccgccaggac aagaattggc
    11701 gtcaaaaggg actctttgat agaactgcga ggtgctccct tgagaatctc tttcattatt
    11761 tttttaggat tcttacctag ataatcctct gcactcatgc catctcgttc tgcttctgag
    11821 aaatctagca tcatcaaata gatctgctct ctgagatagt cttcatcata gagaaatcca
    11881 gcaagattaa aactttccca caactcctca aaatactttt gattctcctc agaaaactca
    11941 tgtagcaaag cgcttgtttc ttcgtaatac ttcattttct tcatggttta acccccattc
    12001 ttaatccctt ctactttttg actcaaatcg tcccattgct gccaaaagac tgagacacgc
    12061 tcttctcctt ctttcgttaa tgaaaaatac ttccgatctg gaccatctgg cgacgggcgc
    12121 atgtcgcctc ttatccattg atttttttct aacttttgca acaaaggata aatagttcct
    12181 ggaacgatag tatcaaatcc agcctctcgc aaagtctgaa ccaactcata accataccgc
    12241 tctttttgac caatcatatc caagacacaa ccttcaagaa caccttttaa tagctgagtt
    12301 tctttcatca cttctccctt ctaatctatt ttgtaatacc tactagtaac ttcacctata
    12361 gtatatcact tctacactag tttgtaaagc ataatagtta atactcttcg aaaatctctt
    12421 caaaccacgt cagcgtcgcc ttaccgtatg tagggttact gacttcgtca gtttcatcta
    12481 caacctcaaa accatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaaccat
    12541 gttttgagct gacttcgtca gtttcatcta caacctcaaa accatgtttt gagctgactt
    12601 cgtcagttct atctacaacc tcaaaacagt gttttgagca acctgcggct agcttcctag
    12661 tttgctcttt gattttcatt gagtataaat aaaaaaacag aactagtctg aactagtcct
    12721 gtctactttt acccaatcac acttccattt ggtacagctg gatcaactgt gagaagggtt
    12781 aatttgccat catgttcagc tgatagaatc ataccctggc tgacatattt tttcatcatt
    12841 ttacgtggtt tgaggttagc aatgatttga actttcttgc cgaccaattc ttgttcattt
    12901 ggatagtatt ttgcaattcc tgaaagaatc tgacgctctt ctccatcacc agcatccaag
    12961 cggaattgaa gcaacttatc tgaaccttct actttagaca cttctttgac ttctgccaca
    13021 cggatttcaa ccttgtcaaa gtcttcaaac ttgatttcat ccttgtttag tttgagctca
    13081 acttcgtccg gattccattc tttttcgact gctggtttat tgccttccat ttgttccttg
    13141 atataggcga tttcttcttc catatttaga cgtggaaaga taggtgttcc tttggcaact
    13201 acagtcacat ctgctgggaa gtcagccaaa ctcaagtttt caagactaga aacttcttcc
    13261 aaaccaagtt gagtcaaaac tgcacgacta gtttccatca taaatggttc aatcaagtga
    13321 gcaactacac gaatgctggc tgccaagtgg ctcatgacac ttgccaattg gtcacgaaga
    13381 gcttcatcct tggccaagac ccatggtgcg gtctcatcga tgtatttatt ggtacgagag
    13441 atcagagtcc agactgcttc aagcgcacgt ggatagtcaa ctgcttccat gtgtgtatgg
    13501 aagtctgcga ttgattgttc tgcaacctca gcaagaacat gatcaaattc agtcacacct
    13561 tctacatagg cagggatttg tccatcaaag tacttattaa tcatggaaac cgtacggtta
    13621 aggaggttcc caaggtcatt agccaattca tagttgatac ggccgacata gtcttcagga
    13681 gtaaaggttc cgtctgaacc aactggaagg ttacgcatga ggtagtaacg aagtggatct
    13741 agtccataac gctctaccaa catttcaggg taaacgacat tcccttttga cttagacatt
    13801 tttccgtctt tcatgacaaa ccaaccatgg gcaatcaaac gatcaggtaa tttaacatcc
    13861 aacatcataa gaaggattgg ccagtagata gagtggaagc gaaggatgtc ttttcctacc
    13921 atatggaaga ctgttccatt ccagaacttg tcaaagttac catgttcgtc ttgagcgtag
    13981 ccaagagctg tcgcatagtt aagaagggca tcaatccaaa cgtagacaac gtgttttgga
    14041 tttgatggga caggcactcc ccatgtaaag gttgtacgag ataccgccaa atcttccaaa
    14101 cctggctcga tgaagttgcg tagcatttca ttaaggcgac cctctggcgt gataaattca
    14161 ggatgagctt tgaaaaattc gaccaaacgg tcttggtatt tgctaaggcg aaggaagtat
    14221 gattcttcag aaacccattc aacctcatga cctgatggag caataccacc agtcacattt
    14281 ccagcttcat cacggaaaac ttctgccagc tggctttctg taaagaattc ttcgtctgat
    14341 actgaatacc aaccagagta ttcacccaag tagatatcat cttgagcaag taaacgttca
    14401 aagacctgtg cgacaacttt ttcatggtag tcatcagttg tacggataaa tttatcgtat
    14461 gagatatcta gtaattgcca gagttcttta actccaaccg ccattccatc aacataggct
    14521 tgaggtgtaa taccagcttc ttccgctttc tgctggattt tctgaccatg ttcatcaaga
    14581 cctgtcagat aaaatacatc gtagcccatc aggcgtttgt aacgtgctag gacatcacat
    14641 gcgatagttg tgtaggcaga accaatatga agtttcccag atggatagta aatcggcgtt
    14701 gtaatataaa aatttttttc agacataatt tttcctttcc aggcaaatga aacctgtttt
    14761 tctaacactt cattatatca catttttaat gaatttcgat agggaaatcc atacaaaaac
    14821 aagatagacg agtgtccatc ttgttgatct cattcataac gaagggcttc aattggatca
    14881 agtttcgatg ccttgttggc tggcaagact ccaaaaatca taccaacact agccgaaact
    14941 gcaagactaa atagggcaac tgggattgat actccaactt ctataccttc tattaaacct
    15001 tgcagtaaca aacctgctaa ggcagttaaa ccacttgcaa ttgtcaagcc aattaagcca
    15061 cctaacaagg tcaaaatcat ggattcaatc aaaaactgaa ttaaaatatt ggcacgtgtt
    15121 gcgcccaaag ccttacgaag accaatctca cgagtgcgct ctgtcaccga aaccagcatg
    15181 atgttcatga caccagttcc tccaacaaag agagaaatcc ctgcgatgga actaataatc
    15241 gtcgtcataa aactaaacga ttgttgaatt tctgcaaata caacggactc atctgccacc
    15301 tggtattctc cctgttgtaa gcctgcaagc tctgtcattt ttcgtgccag ttctggaccc
    15361 agagttgggg ttaaactggt atcattcact cgaaagacaa tactagctat ttcatctata
    15421 ttaaaattcg cagcaaggga gatattggta gtaataggca agccaccaaa cccatatatt
    15481 tttgaccttt tagcctccgg actagtataa accccaatga cccggtaact aaatccattg
    15541 acttctacaa ccttgttaat agcctcttga ggagattcaa ataaactaat ggacaattcc
    15601 tcatctagca aaataacact tgcaaactct ttgaaatctt gctctctcag actacgacct
    15661 gcaataattt cattcttaac agcgtccatg taagttctgt ttccacctgt caaattagca
    15721 ttctcaacct ttttatcttg ataggtcaag atggcattcg ttgaattggt tacatagtaa
    15781 ctatccactc ccttcagttt agctgcctct tgaacccagg attcttgcgg ttttggcggt
    15841 tcaacaggaa cttcctcttc ctttccagaa accgtaaaag ctgattgttt ctgagtaaaa
    15901 gacccatctt tacttttttt aggagagaaa aagacgctaa tatttttctg agatttagtc
    15961 atatctttat tgacttgacg agatagggaa tcacccaaag ccataatcac aacaactgat
    16021 gaaacaccga taataatccc aatcatagta agcaaagaac gcatcttgtg agccatgata
    16081 gatgaaaagg caaatttcag attctgcatc ttagttttcc tcctttccta actgagcact
    16141 gtcagacgaa atgaccccat cccgaatgac aatctgacgt ttggcatagg cagcaatctc
    16201 aggctcatgc gttaccatga taatggtttt tccttcttta ttcaaatcaa ccaataattg
    16261 cataatttgg ttacctgttt tggtatccaa ggctcctgtc ggttcatccg ctaggataat
    16321 agaaggattg tttaccaagg cacgcgcaat ggctacacgt tgcttttgac caccagataa
    16381 ttctgaaggt aaatggtgac tacgttctgt caattcaacc ttgtctaaat attcctcagc
    16441 caacttgcga cgttttgaag acgaaactcc tgcgtaaatc aagggcaatt ctacattttg
    16501 cagagcattg agcttcgata gaagaaagaa ctgctgaaag acaaaaccga tttgttggtt
    16561 acggacctta gctagttgtt tttcaccaag cccagccact tcttgacctt caagataata
    16621 ttctccactg gttggtgtat ccaacatgcc aatcgtattc atcagagtgg acttaccaga
    16681 cccagatggt cccatgatgg ctacaaattc accctcattc acttctagat tgatattttt
    16741 gagaacctgc agttcttggt caccattacg gtaacttctg aagatatttt ttagactaat
    16801 tagttgcttc atcagccttc acctcttttc cttcttccaa ggaagatgtt ggattactga
    16861 tgaccttagc accgttcgtt aaaccagaag tgatttcttg attttctgcg tcagcatttc
    16921 ccaatgaaac ctcaactttt ttagcctttt gttgttcatc cacaatccag acataatttt
    16981 tactatcatc cattactaga ctgctaacag gaacaagaat agccttagtt ttgcttttaa
    17041 cctcaatgtt gacagaaaaa ccttgtttca aatcaccaac ctcgcctgtc acatcaatag
    17101 tataagggta tttagaacct gtattattcc cggctgctgg actagctgct tcaccattgt
    17161 ttttaggata gtcagaaata tagcttaatt tcccagtcca ttttttatca ggatacactt
    17221 tagaagtaaa gcttacttct tgacctacag aaaggttggc tagattgtac tcagacaatt
    17281 ctcccttgac ttgtaaattt tcattgctga caatatgaac cataacttga ctcgcccctg
    17341 ttggagattt agaaacattg ctattgactt cgactacagt tccctctagg gtactgagaa
    17401 cagttgttgc atccaattga ctttgagcct tgcttaattg cgctgcagca tctgcacgcg
    17461 catcacgggc atcacccaat tgagcatcaa tagaagcaac agaatttcca gccactggag
    17521 ttgggctttg caccgttgca tcttctcctc ctactggcgc tggtaactgt ggagcctgag
    17581 ctgaagcggc ttcatttcgt gcttgattga gttcattgat atgacgatct gccttagcta
    17641 ctgctcgact agctgaatca taggccgcct gcgcttctga actactgtac ttgactaaag
    17701 cctgcccttc gctgacctta tcgcccacag aaacaaggat ttcatctaaa tcacccttac
    17761 tagcatcaaa ataaacatat tgttcatttt ttgctgttac tgtccctgac aataaaacag
    17821 aggatgccac gcttccttcc ttggcaacaa caagatgagt aggctcatct tttacagcag
    17881 tctgagaagg ttgtctaaaa agtaaaatcc ccccggcacc caatacaact acactcgcag
    17941 caccgattgc tgcatacagt tgccactttt tagctttacc attctttttc ttcataatga
    18001 aactcctttt attttttaca atactttgct attataccaa atttccctcc agcaaacaat
    18061 gcagttcaga tggaaataat ggcagttcag gattaaacaa tcgttcggaa ttttgctttt
    18121 ctattgtact agatatataa tacactatct tgacttattt ggcaagcttt tttgcaattt
    18181 tttgtgcgta gcagattttt gcaatcaaat caaaaaaaga ataaaatata actatcaaaa
    18241 aagacactct actatttaaa agattacaaa ggagttttca atgagagaat atgatatcat
    18301 tgctatcggt ggaggtagcg gaggaatcgc taccatgaac cgtgctggtg aacatggagc
    18361 caaagcagcc gttattgagg aaaagaaatt aggtggaacc tgtgtcaacg tcggttgtgt
    18421 tcctaaaaaa atcatgtggt acggggcgca aatcgctgag actttccatc aatttggaga
    18481 agactacggc tttaagacta ctgatcttaa ctttaacttt gcaaccctac gtcgcaatcg
    18541 tgaagcctac attgatcgcg ctcgttcttc ttatgatggt agttttaaac gcaacggtgt
    18601 agacttgatt gaaggtcatg ctgaatttgt agattctcat actgtaagcg taaatggtga
    18661 actgattcgt gctaaacata tcgtgattgc tacaggtgcc catccaagta ttcctaatat
    18721 tcctggtgct gagctaggtg gctcttctga tgatgtattt gcctgggaag aacttccaga
    18781 gtcaattgcc attctaggcg ctggttatat cgccgttgaa ttagctggcg tactccacac
    18841 ttttggtgtc aagacagatc tctttgttcg ccacgatcgt cctttacgtg gttttgattc
    18901 ctacatcgtt gaaggtttgg tcaaggaaat ggaaagaaca aacttaccac ttcacactca
    18961 caaagtccct gtcaagttag aaaaaactac tgacggcatt accattcatt tcgaagatgg
    19021 tactagtcac acagctagcc aagttatctg ggctacaggt cgccgtccaa acgttaaggg
    19081 cttgcaactt gaaaaagctg gagtgactct gaacgaacgt ggctttatcc aagtagatga
    19141 ataccaaaat actgttgttg agggaatcta tgctctaggt gatgtaacag gtgaaaaaga
    19201 actgactcca gttgcaatca aggctgggcg taccctatca gaacgtctct ttaacggaaa
    19261 aacaactgca aaaatggatt actcaactat tccaactgtt gtcttttcac accctgctat
    19321 cggaactgtt ggtttgacag aagaacaagc tattaaagaa tacggtcaag accaaatcaa
    19381 ggtttacaaa tcaagcttta cttctatgta ctctgcttgc acttgcaacc gtcaagaaac
    19441 tcgtttcaaa ttgatcacag ctggttcaga agaaaaagtt gtcggacttc atggaattgg
    19501 ctacggcgtt gatgaaatga ttcaaggatt tgctgttgct atcaaaatgg gagcaaccaa
    19561 ggctgacttt gacgcaactg tagcgattca cccaactgca tctgaagaat ttgtaaccat
    19621 gcgttaatcg aaacaaaaga agctgatata aatatgtctg cttctttttt aatgattttc
    19681 gaatcaacaa aaaaattatc caacttgact agtcacgaaa atcggctgtt acagtttttg
    19741 ttaccttgtt tttaaaaaat ggttgacatc gactcctata tgagtataat agttagtata
    19801 cattagaaaa gaggttaact attttgaaaa aagctcacgt ttatgctatc cctgctattg
    19861 gggctgctct cattgctgct ttggcacaaa tcagtcttcc aattggacct gttcccttca
    19921 ctctgcaaaa ctttgcaatc ggcttgattg ctactgtctt tagaccgaga gaggctgtac
    19981 tttctgctgg actctatctt cttctaggtg ctatcggtct tcctgtcttt gcaggaggtg
    20041 gagctggttt tcaggcttta gttggcccta ctgcaggcta tctttggttt tatctcgttt
    20101 actctggact tacttcctct ctaaccaaca gcaagagtgg tgttgttaag atttttcttg
    20161 caaacctctt gggtgatgcc cttgtctttg tcggcgggat tctcagcttg catttcctag
    20221 ctggaatggc atttgaaaaa gctcttgttg tgggggttct tccctttatc attccagacc
    20281 ttggcaaact tctggctatt agttttatta gccgtcccct acttcaacgc cttaaaaatc
    20341 aggcttactt tactaactaa aaaaggatac cgagttatca tgactcaata tccttttttt
    20401 tattttgaaa acttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc
    20461 tgctcaaaac actgttttga ggttgtagat gaaactgacg aagtcagtaa ccatacatac
    20521 ggcaaggcaa agctgatgcg gtttgaagag attttcgaag agtattagtt gcctttaaag
    20581 gcatccacca tcgtttgaaa ttcttcattt gagagagtaa tccctttgcc cattttagta
    20641 tggtctggac tccaagcacg aatatcaaac tttgcagggg caccattaaa gctcacacgg
    20701 ttaatttcct tggtccaacc tttttcgttt tcagaaagag tcaacaagtg ctcttcgatt
    20761 tcaaatgtaa attctgccat tttcttctcc ttttttagtt tcattagttt attcgtaaaa
    20821 tcttgtagat tttaggaaaa ttttatataa tattgatata aaagaaggga ggccaatatg
    20881 agacataaat tccagcaagt tctagataaa atacatgatt ttttaaatgg acatgaccaa
    20941 cctgaccaga ctgaaaccaa ctcccttaca gccactattg aagaggctat ccagaaacaa
    21001 accgctgttc accttatctt gtctgagaca agctttacag gtgatatcat caaatatgat
    21061 cagcaaggcc agcaaattat cgtgaaaaat tttgccaaaa atgtgagccg gattatccgt
    21121 ataagcgata ttcaacgcct gcgatttgtc ccctcaactg tccaaacagc ccaaaaaaat
    21181 agatttaaga aagagtgaga tgtagttgct tcatcccact cttttttctt agcgaatttg
    21241 ttcaaaatgt aaatgaactg cgatatgatc tccataacca cttctttcca agtcacgttg
    21301 taaacgatag gaaatgtagt gttctgcaat ggtaatgtaa cctgcgccca ataaacgatg
    21361 ttcaaccata gattgaatca tactgatagt cgcacgttcc accttggctt cttgtaaatc
    21421 caaaactacc ttcttagtga cttgagcaag attttgacgc aaatcatctg tcaaaacata
    21481 aacagtttgg gctgccttca agatggcttg gtaaatctta tctggattaa attcagcaat
    21541 ttcgccatta cgtttgatta cttgcatagg tttctccttt attctttgtt ttctttgatt
    21601 tctgccagca ttttttcttc ttctactgtc agttgataat gttcaagtaa atctggtctg
    21661 cgctcgtagg ttttctttaa actctcgtac aatcgccact gacgaatctt ttcatggtgg
    21721 ccactcatca atacatctgg cacgaccatg cctcgataat catagggacg tgtgtactga
    21781 ggatattcta aaagacctga agaaaaacta tcatcttggt ggctagactc cttgccaatc
    21841 acttctggaa tcaggcgaac tgtagcatca atcatggtca tagctgccaa ttctccacca
    21901 gtgaggacat agtcgcctag ggaaatctca tctgttacca aggtcttaat gcgctcatca
    21961 taaccctcat agtgcccaca gataaagatt agctcttcct cttgagccaa atcttcagca
    22021 taagcctgat caaactgctt tccagcagga tcgaggagaa taacgcgcgg atttttcttt
    22081 tcaatagcat caaaggaatc gaaaataggt tgtgctctga gcaacatgcc ctgaccgcct
    22141 ccgtagggct catcatctac atgacgggcc ttttcagcat tttctcgaaa attatgatac
    22201 tggatatcca agagcccttt ttctcgagcc tttccaacga ttgagtgctc cagtggagaa
    22261 aacatctctg gaaagagggt taaaatatca atcttcatcg tctaaccctt ctaagatttc
    22321 cacatcgacc cgtttatttg gaatatcaac attgagaacc actggtggga tataaggtaa
    22381 aagcaaatca cgtttgcctt ttcgtttgac cacccagaca tcattagcac ctggttgcag
    22441 gatttccttg atggttccaa ccaagctatc accctcatag acttccaaac cgataatctc
    22501 gtgatagtaa aattcaccat cgtctaggtc attcaaatct tcctcagcga ccttgagact
    22561 atatcccttg tacttttcga tagtattgat atggtacata tctttgaatt taataatgtc
    22621 aaagttcttc tgtttacggt ggctagcgat ggtcactgtt tggacaaact gatctttttc
    22681 atcaaacaaa gccagctcag ctcctttttt aaaccgttct tctgcaaaat ccgtcacaga
    22741 caagactcgc atctcaccct gtaatccctg cgtattaacg attttcccaa cattaaagta
    22801 gttcatcttg tctcctgtaa tctccttttt tccatcttat tctgacaatt ctcgaataat
    22861 agccgcaatt ttttccgatt ctgaccattg taaataatgg tgattccctc ctaaaatgag
    22921 tttagtattg gaagtccaat attctgattc tctgtactct ttttctctat aaggctgaca
    22981 aaaaacaaat acaggaatat gagcttctat agatacatcc tcaaaatctt cctcagtaat
    23041 ctctccagat atctgaaatt ctggatcttg attttccaac tctaagcctt tttcttgcat
    23101 taattcccag atttttttat tcgtttcagg actaaatgtt gctcgagtta agttcttaaa
    23161 ataaagttca ggaccacact cgtcaatcag cctcatctgc tcttccattt ctggataagg
    23221 attttctgaa aaatcagcaa acatgacttt tttagttgtc ggttcaattg ctactaaagt
    23281 ctgacgctta attggtttct cgagtaattt gcaagctaaa attccactcc aactatgtgc
    23341 acaaagtata tattcagaaa tccctaattc ttcaagtact tcataaacca catctgcaag
    23401 attatctaga ttttttccag cttggtcatg aatcggactc ctacctgtgt tcggaaaatc
    23461 aattgtcaaa taaccaattg taggaggagg tttttcaagt ataagtgaaa aattttcata
    23521 acttggtagc aaacctgctc cgtttagaca aactagcact ttcttttgct tttgataagc
    23581 aacagagaga ctaccaattt ctgtagatac ttcaaacctc ttcataaaga aatccactca
    23641 ttctatataa tgaattatta aaaatcctta tcctttattt tatcacgttc caaggatttt
    23701 ctcaagttgg aggaagggga caacatctct actttccctt caaaaatcct tccaaatcat
    23761 gtttatgttg gtaattaacg gctgcggttt tgtctttctc aaagacagtc ttggtaaggt
    23821 caatatgatt aatagctacg attgcgacgg tgtagtaaat gatatcagcc agttctctgg
    23881 caagttcctc gttcgaatcc tatcccttct tttcgaccag agcgcctatt caaaacctcg
    23941 gctacttctc cgacttcctc cactaacttc ataaagagac cttcatcagt ccgagactgc
    24001 tgttaatgtt cgagtaagta gtcttggaat tgcctaaacg ttcaatcttt tatagtatat
    24061 tgaaactaga atagtacacc tttacttcta aaacattgtt agaaatcgat ttgactgtcc
    24121 tgatcgattt gtcctgttat tatttcattt tactatatct tctattccac acaaaaaagc
    24181 gagacatccg tcccgccctt cttatttttc gtcaataacg attcttactt ttttgtattc
    24241 agttgggaca gagtagacaa tcgttcttat cgcagaaata gtgcgaccct tacgaccgat
    24301 tacacgaccc acatcgcttt gatcaagatt caaatgatat tccaaaaatt ctggtgtatc
    24361 ctcaatcttg atagttaagg catctggttg tgaaattaag ggtttcacaa tcgcaataat
    24421 gagattttca atcgtatcca tctgtcaacc tactttaaac ttattttgag aatttagaat
    24481 cgtggaattt tttcaatacg ccttcttttg aaaggatgtt acgtactgtg tctgaaggtt
    24541 gagctccatt agccaaccat gcaagaacgc ggtcttcttt caaagttact tggttttcag
    24601 caacaagtgg gttgtaagtt ccaactgttt cgatgaaacg tccgtcacgt ggtgaacgtg
    24661 aatctgctac gttgatacgg tagaaaggtt ttttcttaga acccatacga gtcaaacgga
    24721 ttttaactgc catttttaaa gtctcatttc tttaattttt tatttcggtg aaatagctga
    24781 gctatttagc acatgttcta ttatagcaga tttctggcat gtgtcaagaa aaaaacttga
    24841 cagactataa atttcttaaa gtatttagaa atttgttaga ataggaaata aatgtttgaa
    24901 agatgctatc cgtgaatact attttagaaa aattttataa agaacaccaa gtcaaaccct
    24961 tcatatcgcc tgaacgagag ttggatactt ggcttctaag tcccaagccc gttcccaagc
    25021 gaaatatgga cttattagca gatgattcac tagcaggaga tatcatttta ctatggagaa
    25081 tccagtttgg tacttttacc actgaaacat gattttaaaa actcccattc gaatatgatt
    25141 caaaattcgc ttagaatcaa tgttttcc
//
LOCUS       DAVGJJ010000023        23415 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000023 DAVGJJ010000000
VERSION     DAVGJJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.023"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..418
                     /locus_tag="VVE35_000847"
     CDS             <1..418
                     /locus_tag="VVE35_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3061071.1"
                     /translation="EQLFDQVATLLEEREIVTPTYREALITREKSFPTGLDMEFLGKD
                     LPNVAIPHTDIVHNLAEKVVVVRLEKPITFHNMIAPDKEVEVSLLFFIINNSSSSQTN
                     ILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            456..761
                     /locus_tag="VVE35_000848"
     CDS             456..761
                     /locus_tag="VVE35_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3061072.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            809..2284
                     /locus_tag="VVE35_000849"
     CDS             809..2284
                     /locus_tag="VVE35_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU3061073.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2310..2381
                     /locus_tag="VVE35_000850"
                     /pseudo
     CDS             2310..2381
                     /locus_tag="VVE35_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2589..9290
                     /locus_tag="VVE35_000851"
     CDS             2589..9290
                     /locus_tag="VVE35_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU3061074.1"
                     /translation="MGEGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVDLTIQANTEKKIAQSIRP
                     VNVVTDLHQEPTLPSTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARARVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEDVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSVGLVVTKGKKED"
     gene            complement(9538..10390)
                     /locus_tag="VVE35_000852"
                     /pseudo
     CDS             complement(9538..10390)
                     /locus_tag="VVE35_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10600..10959)
                     /locus_tag="VVE35_000853"
     CDS             complement(10600..10959)
                     /locus_tag="VVE35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU3061075.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            11051..12007
                     /locus_tag="VVE35_000854"
                     /pseudo
     CDS             11051..12007
                     /locus_tag="VVE35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11991..12548
                     /locus_tag="VVE35_000855"
     CDS             11991..12548
                     /locus_tag="VVE35_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3061076.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12541..12795
                     /locus_tag="VVE35_000856"
     CDS             12541..12795
                     /locus_tag="VVE35_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061077.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12807..14861
                     /locus_tag="VVE35_000857"
     CDS             12807..14861
                     /locus_tag="VVE35_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU3061078.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15040..15900
                     /locus_tag="VVE35_000858"
     CDS             15040..15900
                     /locus_tag="VVE35_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU3061079.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            16111..16821
                     /gene="ccdA2"
                     /locus_tag="VVE35_000859"
     CDS             16111..16821
                     /gene="ccdA2"
                     /locus_tag="VVE35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU3061080.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16841..17407
                     /locus_tag="VVE35_000860"
     CDS             16841..17407
                     /locus_tag="VVE35_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU3061081.1"
                     /translation="MKKWQICVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17418..18530
                     /gene="msrB"
                     /locus_tag="VVE35_000861"
     CDS             17418..18530
                     /gene="msrB"
                     /locus_tag="VVE35_000861"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU3061082.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18798..19535
                     /locus_tag="VVE35_000862"
     CDS             18798..19535
                     /locus_tag="VVE35_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061083.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19532..21223
                     /locus_tag="VVE35_000863"
     CDS             19532..21223
                     /locus_tag="VVE35_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3061084.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21339..21902
                     /locus_tag="VVE35_000864"
     CDS             21339..21902
                     /locus_tag="VVE35_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU3061085.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21924..>23415
                     /locus_tag="VVE35_000865"
     CDS             21924..>23415
                     /locus_tag="VVE35_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU3061086.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKSGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEESKV
                     EQAGEPVAPREDEQAPTAPVEPEKQPEVPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTEVTEAEKVAANTDAKQSEVNEAVEK
                     LTATIEKLVELSEKPILTLTSTDKKILEREAVAKYTLENQNKTKIKSITAELKKGEEV
                     INTVVLTDDKVTTETISAAFKNLEYYKEYTLSTTMIYDRGNGEETET"
BASE COUNT         7167 a         4428 c         5163 g         6657 t
ORIGIN      
        1 agaacaactc tttgatcagg ttgcaacttt attggaagaa cgagaaattg tcactccaac
       61 ttatcgtgaa gccttgatca cgcgtgaaaa gtcatttcca actggtttag atatggaatt
      121 tctaggaaag gacttgccaa atgtagcgat tcctcacaca gatattgttc ataatctagc
      181 tgagaaagtg gtggttgttc gattagaaaa accaataact tttcacaata tgatagctcc
      241 tgataaggaa gtagaagtat ccctactctt ctttatcatt aacaattcaa gttcaagtca
      301 aacaaatatt ctggctcagt tgatggactt tttcacagga aatggacatc ttgaagacct
      361 atcaaaaatt tctgaaccag aaaaacttta tgcttacatt gctgaagcaa ccgcttaatc
      421 ttgtctatta aaaaataaaa tcggaggaaa tccaaatgat taaaattctt gctgcctgcg
      481 gtgcaggtgt taactcaagt caccaaatta aaagtgctct agaagaagaa ctttcaaacc
      541 gtggttacga tgttcactgt gatgcagtca tggtgaaaga tgtaaacgaa gaccttatga
      601 aaggttacga tatctttaca ccaatcgctg caacagacct tggttttgaa ccaggtatcc
      661 cagttatcga agctgggcca atcttattcc gtatcccagc aatgagcgct ccagtatttg
      721 acaatattga agcagctatt aaagaacacg gattaagtta attctaattt gttaacagtc
      781 atataacatc aaaaagggag gaactgttat ggatgcaatc tttgacctaa tcggaaaggt
      841 tttcaatccc atcttagaaa tgggtggacc tgtcatcatg ttaatcattt tgacagtatt
      901 ggctttactt tttggagtga aattctccaa agcgcttgaa ggtggtatca aacttgccat
      961 cgctcttaca ggtatcggtg ctatcatcgg tatgctaaac actgctttct cagcatcact
     1021 agcaaaattc gttgaaaaca ctggtatcca attgagtatt accgacgttg gttgggcacc
     1081 acttgctaca atcacttggg gttctgcttg gacactatac ttcttgctca tcatgttgat
     1141 tgtcaacata gtgatgctag ctatgaagaa aacagataca cttgacgttg atatctttga
     1201 tatctggcac ttgtctatca caggtctctt gattaaatgg tatgctgata acaatggtgt
     1261 gagtcaaggg gtttcactct ttattgctac agcagctatc gtccttgtcg gtgtgttgaa
     1321 aattatcaac tctgacttga tgaaacctac atttgatgac cttcttaacg ccccaagttc
     1381 atcaccaatg acatcaactc acatgaacta catgatgaac ccagttatca tggttttgga
     1441 taagattttt gaaaaattct tcccaggcct tgataaatat gactttgatg ctgctaaatt
     1501 gaacaagaaa atcggtttct ggggatctaa attcttcatc ggtttcatcc ttggtatcgt
     1561 tatcggtatt atgggaactc cacatccaat tgcaggtgtt gcagatgcag ataaatggcg
     1621 tcttgttatc aaaggatggt tgtctcttgg tttgactgcc ggtgtatctt tggaactctt
     1681 ctcacttatc ggttcatggt tcatcgcagc cgtagaacca ctatcacaag gtattacaaa
     1741 cgttgctact aaacgtcttc aaggacgtaa attcaatatc ggtcttgact ggccattcat
     1801 cgctggtcgt gctgaaatct gggcttgtgc caacgtactt gcaccaatca tgttgattga
     1861 agcagtgctt ctttcaaaag ttggaaatgg tatcttgcca cttgcaggta tcatcgctat
     1921 gggtgttact ccagctctct tggttgtaac tcgtggtaaa ttgctccgta tgattatctt
     1981 cggaacactc ttgttgccac tcttccttct ttcaggtacc cttattgcac catttgcaac
     2041 agaacttgct aaaggtgtag gtgccttccc agaaggtgtg agccaaactc aattgattac
     2101 tcactctact cttgaaggac caatcgaaaa acttcttggt tggacaattg gtaacactac
     2161 aactggtgat atcaaagcaa tccttggtgc agtagtcttc cttgtattct atatcggtat
     2221 ctttgcttgg tacagaaaac aaatgatcaa acgtaacgaa gagtacgcag caaaagcaaa
     2281 ataatgcgct cctataaaat ataaacttaa tgaaaatcaa agagcaaact aggaagctag
     2341 ccgcaggttg ctcaaaacac agttttgagg ttgtagataa gactgacgaa gtcagctcaa
     2401 aacatggttt tgaggttgta gatgaaactg acgaagtcag taatcatatc tacggcaagg
     2461 cgacgctgac gaagtttgaa ttggattttc gcagagtgta aattgttgaa actaggttgt
     2521 cataccatgt ataccacttg ggctttggta gtagctaact gcactaaata taatataagg
     2581 agggttttat gggggaaggc cattggaatc ggaaaagagt ttatagcatt cgtaagtttg
     2641 ctgtgggagc ttgctcagta atgattggga cttgtgcagt tctattagga ggaaatatag
     2701 ctggagaatc tgtagtttat gcggatgaaa cacttattac tcatactgct gagaaaccta
     2761 aagaggaaaa aatgatagta gaagaaaagg ctgataaagc tttggaaact aaaaatgtag
     2821 ttgaaaggac agaacaaagt gaacctagtt caactgaggc tattgcatct gagaagaaag
     2881 aagatgaagc cgtaactcca aaagaggaaa aagtgtctgc taaaccggaa gaaaaagctc
     2941 caaggataga atcacaagct tcaagtcaag aaaaaccgct caaggaagat gctaaagctg
     3001 taacaaatga agaagtgaat caaatgattg aagacaggaa agtggatttt aatcaaaatt
     3061 ggtactttaa actcaatgca aattctaagg aagccattaa acctgatgca gacgtatcta
     3121 cgtggaaaaa attagattta ccgtatgact ggagtatctt taacgatttc gatcatgaat
     3181 ctcctgcaca aaatgaaggt ggacagctca acggtgggga agcttggtat cgcaagactt
     3241 tcaaactaga tgaaaaagac ctcaagaaaa atgttcgcct tacttttgat ggcgtctaca
     3301 tggattctca agtttatgtc aatggtcagt tagtggggca ttatccaaat ggttataacc
     3361 agttctcata cgatatcacc aaataccttc acaaagatgg tcgtgagaat gtgattgctg
     3421 tccatgcagt caacaaacag ccaagtagcc gttggtattc aggaagtggt atctatcgtg
     3481 atgtgacttt acaagtgaca gataaggtgc atgttgagaa aaatgggaca actattttaa
     3541 caccaaaact tgaagaacaa caacatggca aggttgaaac tcatgtgacc agcaaaatcg
     3601 tcaatacgga cgacaaagac catgaacttg tagccgaata tcaaatcgtt gaacgaggtg
     3661 gtcatgctgt aacaggctta gttcgtacag cgagtcgtac cttaaaagca catgaatcaa
     3721 caagcctaga tgcgatttta gaagttgaaa gaccaaaact ctggaccgtt ttaaatgaca
     3781 aacctgcctt gtacgaattg attacgcgtg tttaccgtga cggtcaattg gttgatgcta
     3841 agaaggattt gtttggttac cgttactatc actggactcc aaatgaaggt ttctctttga
     3901 atggtgaacg tattaaattc catggagtat ccttgcacca cgaccatggg gcgcttggag
     3961 cagaagaaaa ctataaagca gaatatcgcc gtctcaaaca aatgaaggag atgggagtta
     4021 actccatccg tacaacccac aaccctgcta gtgagcaaac cttgcaaatc gcagcagaac
     4081 taggtttact cgttcaggaa gaggcttttg atacttggta tggtggcaag aaaccttatg
     4141 actatggacg tttttttgaa aaagatgcca ctcacccaga agctcgaaaa ggtgaaaaat
     4201 ggtctgattt tgacctacgt accatggtcg aaagaggcaa aaacaaccct gctatcttca
     4261 tgtggtcaat tggtaatgaa ataggtgaag ctaatggtga tgcccactct ttagcaactg
     4321 ttaaacgttt ggtcaaggtt atcaaggatg ttgataagac tcgctatgtt accatgggag
     4381 cagataaatt ccgtttcggt aatggtagcg gagggcatga gaaaattgct gatgaactcg
     4441 atgctgttgg atttaactat tctgaagata attacaaagc ccttagagct aagcatccaa
     4501 aatggttgat ttatggatca gaaacatctt cagctacccg tacacgtgga agttactatc
     4561 gccctgaacg tgaattgaaa catagcaatg gacctgagcg taattatgaa cagtctgact
     4621 atgggaatga tcgtgttggt tgggggaaaa cagcaaccgc ttcatggact tttgaccgtg
     4681 acaacgctgg ctatgctgga cagtttatct ggacaggtac ggactatatt ggtgaaccta
     4741 caccatggca caaccaaaat caaactcctg ttaagagctc ttactttggt atcgtagata
     4801 cagccggcat tccaaaacat gacttctatc tctaccaaag ccaatgggtt tctgttaaga
     4861 agaaaccgat ggtacacctt cttcctcact ggaactggga aaacaaagaa ttagcatcca
     4921 aagtagctga ctcagaaggt aagattccag ttcgtgctta ttcgaatgct tctagtgtag
     4981 aattgttctt gaatggaaaa tctcttggtc ttaagacttt caataaaaaa caaaccagcg
     5041 atgggcggac ttaccaagaa ggtgcaaatg ctaatgaact ttatcttgaa tggaaagttg
     5101 cctatcaacc aggtaccttg gaagcaattg ctcgtgatga atctggcaag gaaattgctc
     5161 gagataagat tacgactgct ggtaagccag cggcagttcg tcttattaag gaagaccatg
     5221 cgattgcagc agatggaaaa gacttgactt acatctacta tgaaattgtt gacagccagg
     5281 ggaatgtggt tccaactgct aataatctgg ttcgcttcca attgcatggc caaggtcaac
     5341 tggtcggtgt agataacgga gaacaagcca gccgtgaacg ctataaggcg caagcagatg
     5401 gttcttggat tcgtaaagca tttaatggta aaggtgttgc cattgtcaaa tcaactgaac
     5461 aagcagggaa attcaccctg acagcccatt cagatctctt gaaatctagt caagtcactg
     5521 tctttactgg taaaaaagaa ggacaagaga agactgtttt ggggacagaa gtagcaagag
     5581 ttcgtacatt aattggtaaa gaaccaaaaa tgccgaaaac agtaggtttt gtatacagcg
     5641 atggtagtcg tgaaaaactt ccagtaactt ggtctcaagt cgatgttagt caagcaggag
     5701 ttgtgactgt taaaggtaca gcaaatggac gtgaggtaga ggctcatgtc gaggttctag
     5761 caattgcgaa agaattacca acagttaaac gtattgctcc aaatactgac ttgaattctg
     5821 tagacaaatc tgtttcttat gttttgactg atggaagtgt acaagagtac gaagtagata
     5881 gctgggagat tacggaagta gataaagcca aactttcagt agctggatca cgtattcaaa
     5941 tgactggtca gttagctgga gaaactattc atgcaaccct tgtggtagaa gaaggaaatg
     6001 ctgcagcacc tgtagtgcca actgttactg ttggaggtga ggcagtaaca ggtcttacta
     6061 gtcgacaacc aatgcaatat cgtactctat cttatggtgc ccaactgcca gaagtcacag
     6121 caagtgctga aaatgctgat gtgacagttc ttcaagcaag cgcagcaaac ggcatgcgtg
     6181 cgagcatctt tattcaacct aaagatggtg gccctcttca aacctatgca attcaattcc
     6241 ttgaagaagc accaaaaatt gctcacttga gcttgcaagt ggaaaaagct gacagtctca
     6301 aagaagacca aactgtcaaa ttgtcggttc gagctcacta tcaagatgga acgcaagctg
     6361 tattaccagc tgataaagta accttctcta caagtggtga aggggaagtc gcaattcgta
     6421 aaggaatgct tgagttacat aagccaggag cagtcactct caaagcggaa tatgagggag
     6481 ctaaaggcca agttgatctc actatccagg ccaatactga gaagaagatt gcgcaatcta
     6541 tccgtccagt aaatgtagtg acagatttac accaagaacc tactcttccg tcaacagtaa
     6601 cggttgagta tgacaaaggt ttccctaaag ctcacaaagt cacttggcaa gctattccga
     6661 aagaaaaact agactcctat caaacctttg aagtactagg taaagttgaa ggaattgacc
     6721 ttgaagcgcg tgcaagagtc tctgtagaag gtatcgtttc agttgaagaa gtcagtgtga
     6781 caactccaat cgcagaagca ccacaattac cagaaagcgt tcggacatat gattcaaatg
     6841 gtcacgtttc atcagctaag gttgcatggg atgcgattcg tccagagcaa tacgctaagg
     6901 aagatgtctt tacagttaat ggtcgcttag aaggtacgca attaacaact aaacttcatg
     6961 ttcgcgtatc tgctcaaact gagcaaggtg caaacatttc tgaccaatgg accggttcag
     7021 aattgccact tgcctttgct tcagattcaa atccaagcga cccagtttca aatgttaatg
     7081 acaagctcat ttcctacaat aaccaaccag ccaatcgttg gacaaactgg aatcgtacta
     7141 atccagaagc ttcagtcggt gttctgtttg gagattcagg tatcttgagc aaacgctccg
     7201 ttgataatct aagtgtcgga ttccacgaag accatggagt tggtgcaccg aagtcttatg
     7261 tgattgagta ttatgttggt aagactgtcc cgacagctcc taaaaaccct agttttgttg
     7321 gtaatgagga ccatgtcttt aatgattctg ccaactggaa accagttact aatctaaaag
     7381 cccctgctca actcaaggct ggagaaatga accactttag ctttgataaa gttgaaacct
     7441 atgctgttcg tattcgcatg gttaaagcag ataacaagcg tggaacgtct atcacagagg
     7501 tacaaatctt tgcgaaacaa gttgcggcag ccaaacaagg acaaacaaga atccaagttg
     7561 acggcaaaga cttagcaaac ttcaaccctg atttgacaga ctactacctt gagtctgtag
     7621 atggaaaagt tccggcagtc acagcaagtg ttagcaacaa tggtctcgct accgtcgttc
     7681 caagcgttcg tgaaggtgag ccggttcgtg tcatcgcgaa agctgaaaat ggcgacatct
     7741 taggagaata ccgtctgcac ttcactaagg ataagaactt actttctcat aaaccagttg
     7801 ctgcggttaa acaagctcgc ttgctacaag taggtcaagc acttgaattg ccgactaagg
     7861 ttccagttta cttcacaggt aaagacggct acgaaacaaa agatttgtca gttgaatggg
     7921 aagaagttcc agcagaaaat ctgacaaaag caggtcaatt taccgttcga ggccatgtcc
     7981 ttggtagtga tcttgttgct gaggtcactg tacgagtgac agacaaacta ggcgaagctc
     8041 tttcagataa ccctaactat gatgaaaaca gtaaccaggc ctttgcttca gcaaccaatg
     8101 atattgacaa aaactctcat gaccgcgttg actatctcaa tgacggagat cattcagaaa
     8161 atcgtcgttg gacaaactgg tctccaacac catcttctaa tccagaagta tcagcgggtg
     8221 tgatcttccg tgaaaatggt aagattgtag aacggactgt tgcgcaagcc aaacttcact
     8281 tctttgcaga tagtggtacg gatgcaccaa ctaaactcgt tttagaacgc tatgtcggtc
     8341 cagagtttga agtgccaacc tactattcaa actaccaagc ctacgacgca gaccatccat
     8401 tcaacaatcc agaaaattgg gaagctgtgc cttatcgtgc ggataaagac attgaagccg
     8461 gtgatgaaat taacgttacc tttaaagctg tcaaagccaa agccatgaga tggcgtatgg
     8521 agcgtaaagc agataagagc ggtgttgcga tgattgagat gaccttcctt gcaccgagtg
     8581 aattgcctca agaaagtacg caatcgaaga ttcttgtaga tggaaaagaa cttgctgatt
     8641 tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg tcaacggcca aaagtctcag
     8701 ttgaagaaaa taatcaagta gcttcaactg tggtagatag tggagaagat agccttccag
     8761 tacttgttcg cctcgtttca gaaagtggaa aacaagttaa ggaataccgt atccagttga
     8821 ctaaggaaaa accagtttct gagaagacag ttgctgctgt acaagaagat cttccaaaac
     8881 tcgaatttgt tgaaaaagat ttggcctaca agacagttga gaaaaaagat tcaacactgt
     8941 atctaggtga aactcgtgta gaacaagaag gaaaaactgg taaagaacgt atctttacag
     9001 cgattaatcc tgatggaagt aaggaagaaa aactccgtga agtggtagaa gctccgacag
     9061 accgcatcgt cttggttgga accaaaccag tagctcaaga agctaaaaaa ccacaagtgt
     9121 cagaaaaagc agatacaaaa ccaattgatt caagtgaagc tagtcaaact aataaagccc
     9181 agttaccaaa tacaggtagt gcggcaagcc aagcagcagt agcagcaggt ttagctcttc
     9241 taggtttgag tgtaggatta gtagttacta aaggtaaaaa agaagactag aaattttgat
     9301 accttcttta tcattaaaga ccaaagaaaa actcttgtcc taaatagaag ataaagagtc
     9361 tggaacaaaa tagttctcaa ccacaaaaaa gctagatatt tccaattgtg gaactctagc
     9421 tttttaattt tgagtcgttc tcttattgta tagtagattg aaactagaat agtacacttc
     9481 tacttctaaa acattgttag aaatcgattt gactgtcctt atcgatttgt cctgttctta
     9541 tttcatttca ctatgtttta ggaggttatt ggccataagt accaatccca tgtcaattct
     9601 cacttgacgc ttgcctctca gattacatct cttgtaaccc aaacaagcct ttatctgccc
     9661 aaagacaggt tccacatcaa tcttgcgttg agcgaaaatc tgtctatctt ggggagataa
     9721 aagcgcctga cattctttag ctttcaagtt ttgatagcgt tcgttcatat acagtccctt
     9781 ttgaggggct gattcaggtt catcagcgta gtaaaccttg atttcctgtt gaaagtctgt
     9841 ctgtgttggt acttgatatg gtgaaaacga tagcaccagc catcaggatg tatgtagcta
     9901 tcctccttgt cattatagtg ccaattcgct aagtttttag ctgactcttt ataccctctc
     9961 ttttgtttct tatcaaacat ggcatattta atcagaaggg gagtgatttc ttcttgaaaa
    10021 tagacttgta tctgttcttg aagtttggcg gagaatttct ctgtcgtttt cttccacaca
    10081 aaactatact tgttggcatt ggcttcaatc ttagttccgt caataaacaa acaatctaag
    10141 gtcactaact cttccatttt tcaatttttc gaccagagaa aatcccttgt gaataggcaa
    10201 atagaagagt agatacaagc attttagggt gataagacgg gcgaccaaag gcatgataga
    10261 aggcgtagaa gtgacgttcc tccaaggtat tcaccacttt ttcaatagta aagacgagat
    10321 ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt tgtttgattt gtgttatagt
    10381 gaatatgcat gggatagcct ttctaaatgg tttattaagc aattctattt taccaagact
    10441 atcgtaacca tgcaaaaaag caacggcaaa tccgttgcct ttttggatat aagagactat
    10501 tgtcccagac tcttttcttt gtataaaatt gtatagtgtt attgtctata ctattttgta
    10561 gagaaagcaa aaatccctag aaaatgctta gggattcgac tatttcatta agaacaattc
    10621 aacaatcatt tctatataca tgataagggt taataagaat cctgcacgcc agaagaattt
    10681 gatgaattta ggatagtaaa agctgcgttt tttcaaaagg aagaaaaaga cgagaataat
    10741 ggctaggaac gagagggcta gccccagtct agggaggaaa ttatgggtaa aggttttagc
    10801 tgtaatcagg taatactcaa ataccaagac tggaaaagcc aaatccgcaa agtttcgtcc
    10861 tagttttttt aatctaaaaa gtttggttag gataatgcag acgactaagg tcagtatcaa
    10921 taataaaata gatgctaatt tcattaaaat catacccata ttgtatcata aaaaaaggct
    10981 aatggaaacg agaaacagag gaatttatag gaaagtcagg cttttgagat tgtgggtgat
    11041 ttttgttata atgaaagtca tgaaatctta taataccttg aatgattatt atcgaaaact
    11101 ctttggataa aagactttta aagtccctat tgatgcggga tttgactgtc ccaatcgtga
    11161 tggaactgtg gctcatggag gctgtacttt ttgtacggtt tccgattctg gagatgctat
    11221 tgtagcccca gatgcgccta tccgtgagca attttataag gaaattgact ttatgcaccg
    11281 taagtggccg gatgttcaga agtatctggt ttattttcaa aattttacca acacccatga
    11341 aaaggtggaa gttattcgag agcgttatga acaggctatc aatgagccag gtgtggtagg
    11401 tatcaatatt ggaacgcgcc cagactgttt accagacgaa accatcgaat atttggctga
    11461 gttatcggag tgcatgcatg tgatggttaa attgggcttg tagactactt atgaagcaac
    11521 ttctgacctg attaaccgtg cgtattctta tgaattgtag gtggaaacgg tcaagcgttt
    11581 gagaaagtat cccaagattg agattgtttc ccatttgatc aatggtttgc ctggtgaaac
    11641 ccatgagatg atggttgaaa atgtccgccg ttgtgtcacg aataacgata ttcaagggat
    11701 taaactgcat ttgctccatc ttatgacaaa tactcgtatg caacgagatt accatgaggg
    11761 acgtttacaa ttgatgagtc aggacgaata tatcagggtc atctgtgacc aactggaaat
    11821 cattcccaaa catatcgtca tccatcgaat cacaggagat gcacctaggg atatgctgct
    11881 tgggcttatg tggagcctca ataaatggga agctctcaac agcattgaga tggagatgcg
    11941 acgtcgtgga agtgttcaag gatgcaaggc tgtaaaacag gagtttgaaa atgaaaagac
    12001 cacttgagat ggcacatgat tttttggctg aggtcgtgac aaaagaggat gtcgtagtgg
    12061 atgcgactat gggaaatggt catgacacgc tttttttagc caagctagcc aagcaagtct
    12121 atgcctttga tattcagaag caagccttgg aaaagaccca agagcgtttg catcaggctg
    12181 acttgacaaa tgcccagtta atcttgcaag gccatgagac actggaccag tttgtgacaa
    12241 aagctaaggc agggattttt aatctgggct atttgccgtc agctgataag tctgtcatca
    12301 cccgaccgca gacaacgatt gaggcattag aaaagctatg tggcttactt gtcaaaggtg
    12361 gacgaattgc tattatgatt tactatggtc atgaaggagg cgatctcgag agagatgctg
    12421 tcttggattt tgtgatccag ttgaaccaac aagagtacac agctgccatt taccgaactt
    12481 taaaccaagt caacaacccg ccgtttttag tgatgattga aaaattagag agatacagac
    12541 atggataaac aatacctaca cgaaaagctg gatgccatgc gccagaattt tgttgaatca
    12601 acccaccacg aacgagcggt gggcgtgcta gaccaagcgc atatgagcaa aaaaatgctt
    12661 aaaatcaaga aaaaattagt tgctcttgaa atggaacggt gccagagaaa aattgagcac
    12721 aaagactgtt ccaagattga ccaaaaaatc aaagagcaga aggagatatt tgaatcctgt
    12781 tgtaaaaaag attaaggagg acgtgcgtgg aattactgat ttacctcatc ctatttttac
    12841 tggtcttgat tgtctcaagt acaaccaata agctcctacc ctttttgcct ctccctttgg
    12901 ttcagattct tttgggaatt gtgattggtc tctttttacc caatactgac tttcacctta
    12961 atacggagtt gtttttggca ctggttatcg gacccttgct tttccgagag gctgaagaag
    13021 cagatgttac ggctatttta aaacactggc gaatcattgt ttatctcata tttccagtga
    13081 tttttatctc gaccctgagt ttgggtggct tggcccatct tctttggttc agccttccct
    13141 tggcagcttg cttggctgtt ggggcagccc ttggtcctac ggacttggtg gcctttgcct
    13201 ctctttcgga gcgttttagc tttcctaagc gcgtgtccaa tattcttaag ggcgaaggac
    13261 tcttgaatga tgcttctggt ttggtggctt ttcaggtagc tttgacagct tggacaactg
    13321 gagctttttc tctggggcaa gctagcagtt cgctcatctt ttcaattcta ggcggttttt
    13381 taattggatt tttaacagct atgaccaacc gcttcctcca taccttcttg ctaagtgtgc
    13441 gcgcaacgga tattgccagt gaacttttat tagagttgag tttgcctcta gtgaccttct
    13501 ttctggcaga agaagtccat gtttcaggga ttattgccgt cgtagttgct ggaattttaa
    13561 aggcaagtcg cttcaagaaa atcacgctcc tcgaagccca agtggatacg gtgaccgaga
    13621 cggtctggca tacagtgacc tttatgctca acggttctgt ctttgtgatt ttagggatgg
    13681 agttggaaat gatagcagaa cctatcttga ccaatccaat ctataatcct ctacttttat
    13741 tgctatctct catcgccctt acctttgtcc tctttgtcat tcgttttatt atgatctatg
    13801 gctattatgc ctatagaacc cgacgcctca agaaaaagct aaataagtat atgaaggaca
    13861 tgtttctctt gaccttttca ggtgttaagg gaacggtgtc gattgctacg attctcttga
    13921 taccaagtaa tctagaacag gagtatcctc tcttgctttt ccttgttgca ggtgtgacgc
    13981 ttgtcagctt tttaacaggt ctcttggtct tgcctcatct ttctgatgaa gaggaagaaa
    14041 gcaaggatta tctcatgcat atcgccattt tgaatgaagt aacgctagag ttggaaaaag
    14101 agttggaaga caccagaaat aaacttcccc tctatgcggc tattgacaat tatcatggac
    14161 gtattgaaaa tctcatttta agccaagaaa accaggatga tcaagaagac tgggctgctt
    14221 tgaaactctt gattcttagt attgaaagtg atggtttgga acaggcctat gaagagggga
    14281 acattagcaa tcgtgcttac cgagtttacc aacgttatct gaaaaatata gaacaaggaa
    14341 tcaatcgtaa acttgcctca agactgacct attattttct tgtttccttg aggattttac
    14401 gttttctcct tcatgaagtt tttactcttg gaaagacctt ccgtagctgg aaggacaagg
    14461 agcaaagccg tctccgtgct cttgattatg accaaattgc agagctctat cttgccaata
    14521 cagagatgat tattgaaagt ttggaaaacc tgaagggagt ctacagacgc tctttgatta
    14581 gttttatgca ggagtctcgt cttcgagaaa cagctattat cagcagtggt gcctttgtcg
    14641 aacgggttat caatcgtgtc aaacccaaca atatcgatga aatgctgaga ggctattatc
    14701 tggagcgcaa gttgattttc gaatacgaag aaaaacgatt gattacgact aagtatgcca
    14761 agaaattacg acaaaatgta aataacttag agaactattc cttgaaggaa gctgccaata
    14821 ccctaccgta tgatatggtg gaattggtaa gaagaaatta gttaatactc ttcgaaaatc
    14881 tcttcaaacc acgtcagcgt cgccttaccg tatgtatgtg actgacttcg tcagtcttat
    14941 ctacaacctc aaaacagtgt tttgagcagc ttgcggctag ctttctagtt tgctctttga
    15001 ttttcattga gtataagatt ataagtgaag gagtatgaca tgaaaaagtg gtggaaagag
    15061 ctgatagaca aacctttatt aaaagctttt ttgcattatt atcaagcatc agatagtgag
    15121 ttgaccagtg tcgcagtagc ctactattgg ttgatttcga ttttccccct gcttttggtg
    15181 gtggtcaata tcctccccta ttttcagatt cctgtgggag aatttctggg ctttatgaaa
    15241 gacgtcctgc ccccaagcct ctatgaaggt gtggaaaaaa tagccagaga agtcttgacg
    15301 caaccttcaa caggtttatt gagtttttct gttttatcgg cgttatggag tttttcaaaa
    15361 tctatgaatt tcttgcaaaa agcttttaac aaggcttatg gcgtcgaaaa gagtcgtgga
    15421 cttatttccc atcagatgct aagtctccta gtcagttttg gcttgcagct cctctttgcg
    15481 tttgccttgt ttttgatctt atttggtcag atgattttgg ctttacttgc tcattattgg
    15541 acaaaagatg gcattatcta tcaggcttta caagggttgg caggtcctct gatttacgcc
    15601 ctcctctttg ctatcttggt catgctttat tattttcttc ccaatctatc taacaggaaa
    15661 attagctata ccctaccagg cagtgccttt gttctcttag tgattttagg tttactaact
    15721 ctgttctcta gttatctcaa ttactatgtc catcatttgg tggatgtccg aattttaggt
    15781 tccgttcttc ttgttgttat gatgttttgg tttatcttga ttgctaaaat tgttatccta
    15841 ggcgcggtta tcaatgccag tatgcagagt ttgaaagatc cggtctttaa aaaagactaa
    15901 ctatttatcc attataaaag cgcatagtat caagacttta tagaaccttg atactatgcg
    15961 cttttgtgct ttgacagttt tttgtatagt ttttgtccaa gatttctaaa tcatctgatt
    16021 aaattcctat tttcccctat cattgtccct gttttttcga aaatggaagg agtataataa
    16081 atctatcaat aaaatgatag gaggaacttt atgggtcata ttttcttttt tctaagtgtc
    16141 tttttggcag ggattctatc cttcttttct ccttgtatct tacctttgtt accggtctat
    16201 acaggagtgt tactagatga taaggatggt gctcaggctt ctagcggcaa attttcaatc
    16261 tcagttacta gtttattacg aactctggcc tttatagcag gaatttcctt tatatttatt
    16321 ttgttgggct atggagctgg ttttttaggc gatttgcttt atgcttcttg gttccaatat
    16381 cttactgggg caattattat ccttcttggt ttgcaccaaa tggagattct acactttaag
    16441 gggctttata aggaaaagag actacaactg caaggacagg ggcaaaatgg taagggctat
    16501 agtcaggcat ttttattggg cttgaccttt agttttgctt ggacgccttg cgtggggccg
    16561 gttctggggt ctgttttggc cttggcggct tcaggtggtt caggagcttg gcagggagct
    16621 ggtctcatgt tggtgtatac gctgggcttg gcgctaccat tcttgcttct agctctgacc
    16681 tctagttatg ttttgaaaca tttccgaaaa cttcatccct atctcggaat cctcaaaaaa
    16741 gtgggtggtt ttctcattat tgtgatggga ctcttggttc tgtttggaaa tgcttcaatt
    16801 ttaagtcaat tatttgaata aaatggaaag gaatatcaat atgaaaaaat ggcaaatatg
    16861 tgttcttgga gcaggttcgc tcctttgttt gacggcttgt tcaggcaagt ccgtgactag
    16921 tgaacaccaa acgaaagatg aaatgaagac ggagcagaca gctagtaaaa caagcgcagc
    16981 taaagggaaa gaggtggctg attttgaatt gatgggagta gatggcaaga cctaccgttt
    17041 atctgattac aagggcaaga aagtctatct caaattctgg gcttcttggt gttccatctg
    17101 tctggctagt cttccagata cggatgagat tgctaaagaa gctggtgatg actatgtggt
    17161 cttgacagta gtgtcaccag gacataaggg agagcaatct gaagcggact ttaagaattg
    17221 gtataaggga ttggattata aaaatctccc agtcctagtt gacccatcag gcaaactttt
    17281 ggaaacttat ggtgtccgtt cttacccaac ccaagccttt atagacaaag aaggcaagct
    17341 ggtcaaaaca catccaggat tcatggaaaa agatgcaatt ttgcaaactt tgaaggaatt
    17401 agcctaggag gtgtcttatg aatgataagt taaaaatctt cttgttgcta ggagtatttt
    17461 ttctagccat aaccggtttc tatgttctat tgatacgaaa tgcagggcag acagatgcct
    17521 cgcaaattga aaaggcggca gttagccaag gaggaaaagc agtgaaaaaa acagaaatta
    17581 gtaaagacgc agacttgcac gaaatttatc tagctggagg ttgtttctgg ggagtggagg
    17641 aatatttctc acgcgttccc ggggtgacgg atgccgtttc aggctatgca aatggtagag
    17701 gagaaacaac caagtacgaa ttgattaacc aaacaggtca tgcagaaacc gtccatgtca
    17761 cctatgatgc caagcaaatt tctctcaagg aaatcctgct tcactatttc cgcattatca
    17821 atccaaccag caaaaataaa caaggaaatg atgtggggac ccagtaccgt actggtgttt
    17881 attacacaga tgacaaggat ttggaagtga ttaaccaagt ctttgatgag gtggctaaga
    17941 aatacgatca acctctagca gttgaaaagg aaaacttgaa gaattttgtg gtggctgagg
    18001 attaccatca agactatctc aagaaaaatc caaatggcta ctgccatatc aatgttaatc
    18061 aggcggccta tcctgtcatt gatgccagca aatatccaaa accaagtgat gaggaattga
    18121 aaaagaccct gtcacctgag gagtatgcag ttacccagga aaatcaaaca gaacgagctt
    18181 tctcaaaccg ttactgggat aaatttgaat ccggtatcta tgtggatata gcaactgggg
    18241 aacctctctt ttcatcaaaa gacaaatttg agtctggttg tggctggcct agttttaccc
    18301 aacccatcag tccagatgtt gtcacctaca aggaagataa gtcctacaat atgacgcgta
    18361 tggaagtgcg gagccgagta ggagattctc accttgggca tgtctttacg gatggtccac
    18421 aggacaaggg cggcttacgt tactgtatca atagcctctc tatccgcttt attcccaaag
    18481 accaaatgga agaaaaaggc tacgcttatt tactagatta tgttgattaa tactcttcga
    18541 aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt agggttactg acttcgtcag
    18601 ttttatctac aacctcaaag ctatactttg agctgacttc gtcagtttta tttacaacct
    18661 caaagcggta ctttgagaaa cctgtggcta tcttcctagt ttactttttg atttttattg
    18721 agtataagag gtctttccta agcagttaga ggagaatttt gctatactga tactagtaag
    18781 tggtaaagga gcagatcatg acctacacaa tcttaatcgt agaagatgaa tatctggtaa
    18841 gacaaggttt gactaaactg gtcaatgtag cagcctacga tatggaaatc atcggtcagg
    18901 ctgaaaatgg aaggcaggct tgggaattga tccaaaagca ggtgccagat atcattttaa
    18961 ccgatatcaa catgcctcat ctaaatggca tccagttggc cagtctggta cgagaaacct
    19021 atcctcaggt tcatttggtc tttttaacag gttacgatga ttttgattat gccttgtctg
    19081 ctgtcaaact aggtgtggac gactacctgc tcaaaccctt ttctcgtcag gatattgagg
    19141 aaatgttggg gaaaatcaaa caaaaactag acaaggaaga gaaagaagag cagttacaag
    19201 atttattgac taataggttt gaaggaaaca tggcccagaa aatccagtct catctggctg
    19261 atagccaatt tagtttaaag tctttagcca gtgacttagg ttttagtccg acctatctga
    19321 gttccttgat taagaaagag ttgggcttgc cttttcagga ttatctggtg agagaacgtg
    19381 ttaaacaagc caagctcttg cttttaacta cagatctgaa gatttatgag atcgcagaga
    19441 aggttggttt tgaagatatg aactatttta cccaacgttt taagcagatt gcaggtgtga
    19501 cacctcgtca gtttaagaag ggagaagacc gatgaagcgt tcttctcttt tagttagaat
    19561 ggttatttcc atctttctgg tctttctcat tctcctagct ctggttggaa ctttctacta
    19621 tcaatcaagt tcttcagcca ttgaggccac cattgagggc aacagccaaa cgaccatcag
    19681 ccagactagc cactttattc agtcttatat caaaaaacta gaaaccacct cgactggttt
    19741 gacccagcag acggatgttc tggcctatgc tgagaatccc agtcaagaca aggtcgaggg
    19801 aatccgagat ttgtttttga ccatcttgaa gtcagataag gacttgaaaa ctgttgtgct
    19861 ggtgaccaaa tctggtcagg tcatttctac agatgacagt gtgcagatga aaacttcctc
    19921 tgatatgatg gctgaggatt ggtaccaaaa ggctattcat cagggagcta tgcctgtttt
    19981 gactccagct cgtaaatcag atagtcagtg ggtcatttct gtcactcaag aacttgttga
    20041 tgcaaaggga gccaatcttg gtgtgcttcg tttggatatt tcttatgaaa ctctggaagc
    20101 ctatctcaat caactccagt tggggcagca gggctttgcc ttcattatca atgaaaacca
    20161 tgaatttgtc taccatcctc aacacacagt ttatagttcg tctagcaaaa tggaggctat
    20221 gaaaccctac atcgagacag gtcagggtta tactcctggt cacaaatcct acgtcagtca
    20281 agagaagatt gcaggaactg attggacggt acttggcgtg tcatcattgg aaaagttaga
    20341 ccaggttcgg agtcagctct tgtggacctt gcttggggcc agtgtcacat ctcttcttgt
    20401 ttgtctctgc ttagtgtggt tcagtcttaa acgctggatt gctcctttga aggatttgag
    20461 agaaaccatg ttggaaattg cttctggtgc tcaaaatctt cgtgccaagg aagttggtgc
    20521 ctatgaactg agagaagtaa ctcgccaatt taatgccatg ttggatcaga ttgatcagtt
    20581 gatggtagct attcgtagcc aggaagaaac gacccgtcag taccaacttc aagccctttc
    20641 gagccagatt aatccacatt tcctctataa cactttggac accatcatct ggatggctga
    20701 atttcatgat agtcagcgag tggtgcaggt gaccaagtcc ttggcaacct atttccgctt
    20761 ggcgctcaat caaggcaagg acttgatttg tctctctgac gaaatcaatc atgtccgtca
    20821 gtatctcttt atccagaaac aacgctatgg agataagctg gaatacgaaa ttaatgaaaa
    20881 tgttgccttt gataatttag tcttacccaa gctggtccta caaccccttg tagaaaatgc
    20941 tctttaccat ggcattaagg aaaaggaagg tcagggccat attaaacttt ctgtccagaa
    21001 acaggattcg ggattggtca tccgtattga ggatgatggc gttggcttcc aagatgctgg
    21061 tgatagtagt caaagtcaac tcaaacgtgg gggagttggt cttcaaaatg tcgatcaacg
    21121 gctcaaactt cattttggag ccaattacca gatgaagatt gattctagac cccaaaaagg
    21181 gacgaaagtt gaaatatata taaatagaat agaaactagc taactcccag tcaattctgg
    21241 gagttttatg cgtaattggg caaactttct aggttgtcta tcttgctaaa aacaagaaaa
    21301 aacggtatga tagaagagtg acaacagagg taacagagat gaacgagaca cagattcaaa
    21361 gagaaacacg tcaggtagtg gaagatgttt tagaaaagac caatttgaag cagggggccc
    21421 tttttgtttt aggtctatct tctagtgagg tgctaggtgg tcagattggg aaagaatcca
    21481 gtcaagagat tggtgagctg atagtggaga ctatcttggg tattctaggt agtagaggta
    21541 tccatttagc agttcaaggt tgtgagcatg tcaatcgggc tctcgttgtt gaacgtcagg
    21601 tggcagagca gtttggtctg gaaattgtca gtgtccatcc tactcttcat gcaggaggtt
    21661 cggggcagtt ggcagccttt aagtttatgc aggatccagt tgaggttgaa tttatcaagg
    21721 ctcatgctgg attggatatc ggagacactg caattggcat gcatgtcaag catgttcagg
    21781 ttccgattcg ccctattttg agagagattg gtcatgccca tgtaacggca ctggctagtc
    21841 gtccaaaatt aatcggaggt gcgcgtgcgc actatccgca agatgctatt agaaagtctt
    21901 gagaatgaat aaaggagata aaagtgttta aaaaagaccg tttttcaatt cgtaagatta
    21961 agggagttgt aggctctgta tttcttggaa gccttttgat ggctccttct gtagtggacg
    22021 cagccaccta tcactatgta aataaagaga ttatttcaca agaagctaaa gatttaattc
    22081 agacaggaaa gcctgacagg aatgaagttg tatatggttt ggtgtatcaa aaagatcagt
    22141 tgcctcaaac agggacagaa gcatctgttt tgacagcttt tggttatcta tcaggtgaca
    22201 tcctcaaaac gcttggcttg gacactgttt tagaagaaac ctcagcaaaa tctggagagg
    22261 tgactgtagt cgaagttgag actcctcaat caacaacaaa tcaggagcaa gctagaacag
    22321 aaaaccaagt agtagagaca gaggaagctc caaaagaaga agcacctaaa acagaagaaa
    22381 gtccaaagga agaaccaaaa tcggagataa aacctactga cgacaccctt cctaaagtag
    22441 aagaggggaa agaagattca gcagaaccag ctccagttga agaagtaggt ggagaagttg
    22501 agtcaaaacc agaggaaaaa gtagcagtta agccagaaag tcaaccatca gacaaaccag
    22561 ctgaggaatc aaaagttgaa caagcaggtg aaccagtcgc gccaagagaa gacgaacagg
    22621 caccaacggc accagttgag ccagaaaagc aaccagaagt tcctgaagaa gagaaggctg
    22681 tagaggaaac accgaaacca gaagataaaa taaagggtat tggtactaaa gaaccagttg
    22741 ataaaagtga gttaaataat caaattgata aagctagttc agtttctcct actgattatt
    22801 ctacagcaag ttacaatgct cttggacctg ttttagaaac tgcaaaaggt gtctatgctt
    22861 cagagcctgt aaaacagcct gaggtaaata gcgagacaaa taaacttaaa acggctattg
    22921 acgctctaaa cgttgataaa actgaattaa acaatacgat tgcagatgca aaaacaaagg
    22981 taaaagaaca ttacagtgat agaagttggc aaaacctcca aactgaagtt acagaggctg
    23041 aaaaagttgc agctaatacg gatgctaaac aaagtgaagt taacgaagct gttgaaaaat
    23101 taactgcaac tattgaaaaa ttggttgaat tatctgaaaa gccaatatta acattgacta
    23161 gtaccgataa gaaaatattg gaacgtgaag ctgttgctaa gtatactcta gaaaatcaaa
    23221 acaaaacaaa aatcaaatca atcacagctg aattgaaaaa aggagaagaa gttattaata
    23281 ctgtagttct tacagatgac aaggtaacaa cagaaactat aagcgctgca tttaagaacc
    23341 tagagtacta caaagaatac accctatcta caactatgat ttacgacaga ggtaacggtg
    23401 aagaaactga aactc
//
LOCUS       DAVGJJ010000024        23337 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000024 DAVGJJ010000000
VERSION     DAVGJJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.024"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(164..1144)
                     /locus_tag="VVE35_000866"
     CDS             complement(164..1144)
                     /locus_tag="VVE35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU3061087.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGREHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYGHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1438..1629)
                     /locus_tag="VVE35_000867"
     CDS             complement(1438..1629)
                     /locus_tag="VVE35_000867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061088.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTWFEEIFIEY"
     gene            complement(1657..3672)
                     /gene="recG"
                     /locus_tag="VVE35_000868"
     CDS             complement(1657..3672)
                     /gene="recG"
                     /locus_tag="VVE35_000868"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU3061089.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3691..4794)
                     /locus_tag="VVE35_000869"
     CDS             complement(3691..4794)
                     /locus_tag="VVE35_000869"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU3061090.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREVSEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4784..5152)
                     /locus_tag="VVE35_000870"
     CDS             complement(4784..5152)
                     /locus_tag="VVE35_000870"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU3061091.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5191..6222)
                     /locus_tag="VVE35_000871"
     CDS             complement(5191..6222)
                     /locus_tag="VVE35_000871"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU3061092.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6224..7255)
                     /locus_tag="VVE35_000872"
     CDS             complement(6224..7255)
                     /locus_tag="VVE35_000872"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU3061093.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7336..9849)
                     /gene="secA"
                     /locus_tag="VVE35_000873"
     CDS             complement(7336..9849)
                     /gene="secA"
                     /locus_tag="VVE35_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU3061094.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9995..10282)
                     /locus_tag="VVE35_000874"
     CDS             complement(9995..10282)
                     /locus_tag="VVE35_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061095.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAIFPIFGN"
     gene            complement(10293..10943)
                     /locus_tag="VVE35_000875"
     CDS             complement(10293..10943)
                     /locus_tag="VVE35_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3061096.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10947..11588)
                     /locus_tag="VVE35_000876"
     CDS             complement(10947..11588)
                     /locus_tag="VVE35_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061097.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11581..12372)
                     /locus_tag="VVE35_000877"
     CDS             complement(11581..12372)
                     /locus_tag="VVE35_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061098.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAIAYLMFLWLGSILLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12557..12892)
                     /locus_tag="VVE35_000878"
     CDS             complement(12557..12892)
                     /locus_tag="VVE35_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061099.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13032..13367)
                     /locus_tag="VVE35_000879"
     CDS             complement(13032..13367)
                     /locus_tag="VVE35_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061100.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13458..14768)
                     /gene="der"
                     /locus_tag="VVE35_000880"
     CDS             complement(13458..14768)
                     /gene="der"
                     /locus_tag="VVE35_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU3061101.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNKYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14782..15495)
                     /locus_tag="VVE35_000881"
     CDS             complement(14782..15495)
                     /locus_tag="VVE35_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU3061102.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15492..16388)
                     /gene="dnaI"
                     /locus_tag="VVE35_000882"
     CDS             complement(15492..16388)
                     /gene="dnaI"
                     /locus_tag="VVE35_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU3061103.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKFSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16389..17558)
                     /locus_tag="VVE35_000883"
     CDS             complement(16389..17558)
                     /locus_tag="VVE35_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU3061104.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTVVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17559..18032)
                     /gene="nrdR"
                     /locus_tag="VVE35_000884"
     CDS             complement(17559..18032)
                     /gene="nrdR"
                     /locus_tag="VVE35_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU3061105.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18172..18537
                     /locus_tag="VVE35_000885"
     CDS             18172..18537
                     /locus_tag="VVE35_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3061106.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18542..19237
                     /locus_tag="VVE35_000886"
     CDS             18542..19237
                     /locus_tag="VVE35_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061107.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19254..20021
                     /locus_tag="VVE35_000887"
     CDS             19254..20021
                     /locus_tag="VVE35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061108.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20374..21573)
                     /locus_tag="VVE35_000888"
     CDS             complement(20374..21573)
                     /locus_tag="VVE35_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061109.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEV
                     KENKKFIQTIAAGALGFFLYMILIAYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21570..22463)
                     /locus_tag="VVE35_000889"
     CDS             complement(21570..22463)
                     /locus_tag="VVE35_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061110.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILKDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22580..22660
                     /locus_tag="VVE35_000890"
                     /pseudo
     CDS             22580..22660
                     /locus_tag="VVE35_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            23145..23337
                     /locus_tag="VVE35_000891"
                     /pseudo
     CDS             23145..23337
                     /locus_tag="VVE35_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         6864 a         5231 c         4327 g         6915 t
ORIGIN      
        1 taaaacattg ttagaaatcg atttgactgt cctgatcgtt ttgtcctatt cttgtttcat
       61 tttactatat ttccatgata aaacgcatag tatcaagttt ttttaatccc ctgatactat
      121 gcgtgtttgt aatttttaag attttgtgct tagagtcgac tccttatttt agatatttaa
      181 aaggaatctc acttccacag agccagttgt agacttggtc attgacaaat acattcatgg
      241 cttcgtgacc atactcaggc atgatgcgat aggttttatc gcaggtcaga cgattataaa
      301 tcgcaaactg ggtaatggga tagcaaacat cgtcgtccaa gcccgtaatc atcttaacct
      361 caccttggat acgatgggca agatttttga catcgatata ggcaagggtc gccatgattt
      421 cctcctctgt ttcatggaag gggtcgtgaa acttgaaata acggaaaagt tcgtcgtaag
      481 cctcgctagt attaccaatc tcaatcaccc gtctgaagtc tgacaagaag ggataaatgg
      541 caactgtttt ctgaattcga ggattgagcg ctgctgcaac tagagctaga gcccctcctt
      601 gtgaggcacc atagctagaa agacgcttct catcaacctg agacagacta gcaacaattt
      661 cgaccaactg gtaaatatcc agatagacat ccttataaaa gagatgctcc cgaccctcca
      721 cagcaccacg gataatatgc cccttaaccg tatttcctaa aggagaacgc aagccgtctt
      781 gtgagtaacc tgactggccc cgcacatcca tggaaacaac accgtaacca gctacggtga
      841 agcccagcat gtcggcccag tcccagccac gtcccatata accatgaaaa tggaagatta
      901 gtggaacctt ctcctcactc tttggaagaa caatgcgtgc atagaccttt ccttccttgc
      961 ttccttcaaa tgttaactca tagcacttga cttgaggaat gtggaaatct ctttcctcca
     1021 agtggtagga tggaagcgtg gaaacatttt tcacttcccc atcccagaaa gcatcaaagt
     1081 cttccggaac ctcatccctt cctctatagg tcttaatttc ttctaacaaa gctggatttt
     1141 tcatagcatg ctccttttat tttgtaatcg ttaccacaac tgtagcatat ctatgtaatc
     1201 aatgcaagaa agaaggataa atagaaagtg aatttagatt ctttactttt aggatgatgg
     1261 gctgaaagta gttttagaaa cgaaaaagag catttcgctc tctcttctgt ttatactagt
     1321 tttttgcttc cttcttttgg aaagtggttt ggtaatttac tttaatcgtt actgtcatat
     1381 gagagtcctc gtttcttttt gatgacttta gtataagagc caaacctaaa aactagctta
     1441 atactcaatg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtagg tatggttact
     1501 gacttcgtca gtttcatcta caacctcaaa accatgtttt gagctgactt cgtcagtttc
     1561 atctacaacc tcaaaacagt gttttgagca acctgcggtt agcttcctag tttgctcttt
     1621 gattttcatt gagtataagt tttccttaga gaaagcttaa tccagatgtt ctttcttttc
     1681 cagatgaagg gcaatcatgc gccactctgg atcttcttgc caagcttcta tagaactaat
     1741 gtagctagca acctttcttg cttcttctaa aatcggaaaa tcttcgataa tatcagccac
     1801 ttggaactct ggaagtcctg actgtctggt tccaaaaatc tcaccagaac cacgcatttt
     1861 caaatcttcc tccgcaagga caaatccatt ggtcgtttct gtcatgatgc gcatgcggtc
     1921 tttcccagaa tccgtcttgg gattggcaac gagaacagcg taggactgct tgtccccccg
     1981 accgacacga cctctaagct ggtgaagttg actgagaccg aagcgatcgg catccatgat
     2041 aatcatgaca gtcgcattgg gaacgttgac cccaacctca ataaccgtcg tcgaaaccag
     2101 aatatccgtc tttctctcct tgaaatcctg catgatctgg tctttttcgt cactcttcat
     2161 cctaccatgt agaagagcca cctctgcctt gcctgcaaaa tgagtcgtca actcctctga
     2221 taaggcaatg gcatttttca aatctagagc ttctgattct tcaatcaaag gagagatgac
     2281 ataggcttgg gaaccttttt gaatttcccc ctctaaccaa gtcaagacct gaggtagttg
     2341 ctcatgtttg atccagcgcg tcacaatagg cttccgacct gctggcatct ggtcgataat
     2401 ggaaacatcc atatctccaa aggctgtgat ggcaagcgtc cgtggaatgg gagtcgccgt
     2461 catcatgagg acatctggat tgtcaccttt ttcccgtaaa atacgccttt gccctacacc
     2521 aaaacggtgc tgctcatcga taataatcaa accaagacga gcatactcca ccccatcttg
     2581 tatcagagcg tgagttccga taatcaaatc agcctcaccc ttggcaatgg tctccaagac
     2641 ctctctcttt tctgcagctt tcaaggaacc tgtcaagaga gccagtttca aattgggaaa
     2701 aaggttctgt aaactctcaa agtgttgctc tgcgaggatt tctgttggta ccattagggc
     2761 agcctgataa cctgctgtca ctgccgcaaa catggccaag ccagcgacta ccgtttttcc
     2821 actccccaca tccccttgta ggagacgatt catgtggtgg tcggacttca tatcagttaa
     2881 aatttcctgc aaactctttt cctgagcttg ggtcagggca aaaggaagac ttactttaac
     2941 tgctgtcact ttttcctgag accaattcag aaccagacca cttccctgaa ctctattttc
     3001 agacttgagc gtctgcaact gcatttggaa ataaaagagt tcctcaaact tgatacggcg
     3061 aagagcctgc ttgtattctg ccaaatcctt tggaaaatgc attgctcgga ctgcctgaca
     3121 acgggacatg agtttgtatt tgtctagtaa agactggggc agattttctt ctatcaagag
     3181 gtccagtccc tgatcaaaag ccgtcttgat gaccttaacc agactggcct gactgattcc
     3241 ctgagccaga cgatagacag gctggaggtc atcttctacc tgagccagaa ccttcatccc
     3301 agtcagacta gccttagcgc ggtcccattt tccaaagaca gcaagggttg ctcccaactc
     3361 tattttatca gccagatagg gctggttaaa gaaattcacc gcaaaaacga cctctccctg
     3421 cttgagacta aaacgcaggc gattgcgctt gaaaccataa tactggacac tagcaggagt
     3481 cactacctga ccagaaagaa ctgccttctc accgtcttcc agctccagca cctgcttggt
     3541 tttgaagtct tcataacgga aaggaaagta gagcaagaga tcttgcaagt tttcaattcc
     3601 tagtttggca tatttttctg ctgactttgg tcccacacca ggcaagacat gcaagggttg
     3661 atgtagattc atgctccact cctttctttt ctaataatat tctctcggaa tacggtcgct
     3721 gaggaggcaa accacctcat agttaatggt tacgcggtag gtcgctacct gagttgcagt
     3781 gatttcctta tccccattgg agccaatcaa ggttaccttg gttccgagtg ggtaaggctt
     3841 aggcaatcga atagtgattt ggtccatcga aaccctgccg acaattgggc aagcttggcc
     3901 atctaccaag acagagaaat tttgcatgtc tcttgtccat ccatctgcat acccgattgg
     3961 tacggtcgcg atgacttgct cgctatccgc ttgataagtt gctccatagc ccatgcaagc
     4021 tccagctgga actgtcttga catgaaccag agcagactcc aaggtcaagg ccggtatcaa
     4081 atcataaggc aaatccaaga ccgctccact tggattgagg ccatacatgg catctcccat
     4141 acgaaccgca ttgaaaatag tctctacatg ccaaagagtc gttgcagaat tgctagcatg
     4201 aaccagctct ggaacttcct tcatactagc taaaatagtt ttaaaccgtt ctaactgggc
     4261 attaaaatag tcatctgatt cctcatcagc agtagcaaag tgggtaaaga ttccttcaac
     4321 acaaacaccg tgttgttgga gcaaatcttg agcctgctca acctcactga cctctctaaa
     4381 accaatccgt cccatccctg aatcaatctt gaggtggact gtcaatccag ttaggtccac
     4441 ttccttatct aagagtgctt gaatccactc cagtccagcc actgtcaagg tgaagtcata
     4501 ttctttagct agagcaacag cttcgatttc agaaactcct aaaatgagga ttggcttact
     4561 gagtccagct tgtctgagtt caatggcttc atcgatattg gaaacgcaaa agccatcaac
     4621 atcatcttga attgccttgg caacggcaac agctccatga ccataagcat tggccttgac
     4681 cacagcccac ttgagcgttc cttgagggat atgagccccc atttgctgaa tattttgtcg
     4741 aatagctccc agatgaatca gagccttggt tggtctatgt ggactagctt tcatgatttt
     4801 cctccaaaat gacactggct gtcacaaact gatcggtgtg gctgatagac agccaaatct
     4861 ttcctgaaaa tggtgcctga ctaaaataag gcgccccacg ttcattgttc aagacttcca
     4921 aatcctgaaa accgagcttg ctaatgcccg ttcccatagc cttggaaaag gcctccttag
     4981 ccgaccagcg accagctaaa tattctattt gcctgcgtcc tttgagactg gtgaagcgct
     5041 ccatttcctg agcggtcagt acacgcttag caaatccttc atgtcgtgta actgcgcttt
     5101 ctatcgaagc caattcttcg atgtcaattc cgtgtccaac tatcattctc atcaaaagga
     5161 gttgagattc cccaactcca tccttttcac ttattttgtc aaggtaacat aaatctcttc
     5221 taccaaggcc tctgtatttt cccaacctag gcaagggtca gtaatagagc aaccaaagac
     5281 ctctggttgg ttttgacgac catctgctag gtaagattca atcataaatc ctcgaaccgt
     5341 ctttttaatt ttctcattcc aatcacgatt ctgcaaggtc tggcgaacaa ttcgaatctg
     5401 ctccatatat tgcttgcctg agttatcatg gttggtgtca atgaggataa aaggattttc
     5461 aagtcccatg gtttcatagc gttcaatggc ttggagtaga ttttcatagt agtaattcgg
     5521 catataattg ccatactcat tgactgctcc acggaggata acatgggcca aaggattacc
     5581 tgatgtctca acttcctgcc catgataaag gaaggtttgc ttgttttgag cagcatagat
     5641 ggcgttaaac ataacaccca aatttcctga ggttggattt ttcatcccta ctggtgcatc
     5701 aatcccagaa gccacaaagc ggtgctcttg gtcttccaca gaacgagctc caacggcatg
     5761 gtagctgacc aagtcatcca ccaagatcag atttgacgga taaagcatct catctgccgt
     5821 tgtcaaacca gtctctgtaa tcacgcggta gtgcaactgg cgcacagcct gcaagccgtt
     5881 aatcaggctt ggagccttag aagtatctgg ctggtgaacc aaccctttat agccgtctcc
     5941 attggtacga ggcttagcag tatacacgcg catgaccatg aaaatcttat ccgctacctt
     6001 cttttgcaag gcggataaac ggcgagcata ttccaagacc gcctcttcat tatcagaaga
     6061 gcaaggacca atcaccaaga gaatacggtc atcttcccct gaaataatat ctgccaattc
     6121 cctgtcacgg ctttccttga gtctcaaggc ttccgcagac aattgggttt cagccttgat
     6181 gacttccata tcgatttctt gacctttttc aataaatgcc atcttattct cctaacgttt
     6241 tgtaaatttc tctgacaaga gcttctgtgt tatcccaacc caggcaaggg tctgtgatag
     6301 acttaccaaa tacttctggc tcattttgtc gaccatcttc cagataagac tcaatcataa
     6361 aaccacgaac gaactgctta attttttcat tccaagcacg gttaatcaag gtctggcgga
     6421 caattcggat ctgttcaata tactgcttac cagaattgtc atgattggta tcaatgatga
     6481 taaaaggatt ttccaagccc attttctcat actgggcaat ggtatcaatt aaattgtcat
     6541 aatagtagtt gggaatattt tttccatact cattaagagc accacgaaga atagcgtgtg
     6601 aaagcgggtt cccagttgtt tctacttctt ttcctaagaa aaggaaactt tgtttgtttt
     6661 gagcagcata aatcccatta aacatgacat tgagatttcc agaggttgga tttttaaaac
     6721 cagtagaaaa tcctgcccca cttgccacaa agcggtgttg ctggtcttca actgaacggg
     6781 caccaactgc catgtaagaa atcaaatcat ctacaagcgg aaggttttca ggataaagca
     6841 tttcatcagc agttgtcatc cctgtttctg tgatgacacg atagtgaaga tggcgaacgg
     6901 ctttgattcc attgataaga ctaggcgctt ctgtcgcgtt aggctggtga atcaagccct
     6961 tatagccatc tccgttggta cggggtttgg cagtataaac acgcataacc ataaagatac
     7021 gatctgccac ttcttcttgt aggactgcca aacgcttagc gtattcaagg acagcttctt
     7081 cgttgtcaga tgagcatggc ccgattacca agagaattcg ctggtcttct ccacgtataa
     7141 tggcttctag ctcttgatct cgctgtgatt tcctctccaa agcctgacct tctaattttg
     7201 acaaggcacg aacttcttct atatttattt tagagctttt tgctgtaaat accataactc
     7261 atctccttat aaagcaaacg gatacccagt aaaaatttac ttttcacaag atatccgcct
     7321 ctaaactatc tcattttatt gtcttttacc gtgacagttt ttaaatttct taccagaacc
     7381 acatgggcaa agttcattgc gtccaatctg gctcaaatcc aaatcttctg gcatacttgc
     7441 ttggtgagca gcgatattgc gagtcgctgt tgtactgata tgacgttctg cctgtggtct
     7501 ttcttgttca tgaatttgtg ctttcatcat caagcgtgtc acatcaaact caatcgaacc
     7561 aatcatatca ttaaacatac ggaaaccttc tgcctgatac tcaacaacag ggttgttctg
     7621 agcatagcca cgaagtccaa ccgcgttacg caattgatca agggcatcga tatgatctgt
     7681 ccacttgtta tccaccactc gtagaatcaa aactttttgg aattctttaa ctgcttcttc
     7741 atcgcgtagt tttgaaacct gactatcgta aaccttcaag gcacgttgga aaagctcttc
     7801 cttgatggcc ttatcagaca agcctgacaa gtcttccatc gtaatagaat cttcaggaag
     7861 caagttgtac ttagcgaagt tcaaaattgc ctctagtttc tcatcttgtt tagcacgcgc
     7921 atgaccgtca acgacacgac caatcgtgcg tttgatcatt gcctgaattt caggtgccaa
     7981 gtcacggtct gcagtaatga catcataacg ttgagtatag ataatctcac gttgttcacg
     8041 catgacatcg tcgtattgaa ggacttgttt acgggtatcg tagttatttc cttcgacacg
     8101 tttttgcgct gcttcaacct gacgcgtcaa catgcgagac tcaatcgcct cttcagacat
     8161 gttgagacgt tcaaagattc ccttcaagcg ttcagaacca aaacgtttca tcaaatcatc
     8221 ttcaagagat aggtagaatt gtgactcacc tggatctcct tgacgacctg aacgtccacg
     8281 aagctggtta tcgatacgac gactttcatg acgttctgta ccaataacac aaagtcctcc
     8341 aagttcacga acaccttcac caagcttgat gtcggtacca cgacccgcca tgttggttgc
     8401 gatggtaacg gcaccacgtt gaccagcatt catgatgatt tgggcttctc tatagtggtt
     8461 tttggcattc aagacttcgt gaggaacacc agctgcaacc aatttcttag aaatgtagtc
     8521 actagtttca accgctactg taccaaccaa gacaggttga cccttttggt aacgagcctt
     8581 aacgtcttcg acaaccgctt taaacttaga ttcgatactt gcataaagaa ggtctgagtg
     8641 gtcaatacgt tgaacaggac ggtttgttgg gattggaata acacgaatgt tgtagatttc
     8701 acggaattct tcttcctcag tcttacctgt acccgtcata ccagacaatt ttttgtacat
     8761 acggaaaagg ttttggtacg tgattgaggc agatgtcttg gtttcatcct ggattggcac
     8821 accttctttg gcttcaatag cttggtgcaa tccatcagaa taacgacgac cttccatggt
     8881 acgacctgta aattggtcga caatcaagat ttcttgctct tcgctcacca catagtcaat
     8941 atcgagaagc atgatgtagt tggcacgaag ggcgttatcg ataaagtgag tcaaagccac
     9001 gttttcgatg tcatagaggt tttcaagttt gaagtagctt tcagccctgt caatccctga
     9061 atcagacaaa ccaatagtct tagactgcac atcgatgatg tagtcatctt tgttcaaaga
     9121 ttttacatag tggtctgcca tgtgatacaa ctgactggtt tcaaccgcat tggcacctga
     9181 tacaatcaaa ggtgtacgag cctcgtcaat caagatagag tcaacctcat cgaccaaggc
     9241 atagttaagc ggacgttgta ccatgttttc agcgcgaacg accatgttgt cacgaaggta
     9301 gtcaaatccg atttctgagt tagttgagta agtaatatca cactcatagg cttctttttt
     9361 ctccattgga gatttggtag ccaagttaat ccctactgac aaaccaagcc aagagtacaa
     9421 ttcacccatc tcagtcgcgt cacgttctga caggtattca ttaaccgtaa ctacgtgaac
     9481 ccctttacct gaaagggcat tgaggtatac cggcatggtc gcagtcaagg ttttcccttc
     9541 ccctgtacgc atctctggca cgtcaccatg gtgaagaaca atccccccca tgacctgaac
     9601 cttataaggg aagagaccta ggacacgttt ggcaccttca cggacaaccg caaatgcttc
     9661 gtaaagcaat gaatccagtg attctccatt ttgataacgt tccttaaatt caactgtttt
     9721 tgcttttagt tggtcgtcag tcaaagcagc catttggtct tcgtatttga aaaccttgtc
     9781 agccatcttt tccagacgac ggatttctcc tttatcattt tcgataattg tttttaaaat
     9841 attagccatg tttttcctta ctttaaattc cgaatatttt agaatgttcc tttaatttta
     9901 gcacaattac tgattatttt caaggaagaa tccccatttt gaaaaggaaa aagaggttag
     9961 ttttcaagct aacctctccg acttttatga ctgtttaatt gccaaaaatc ggaaaaatgg
    10021 caaatgtgat aaataagtta atccaaagag aaagacagca aatcagtcca aaaacaaaga
    10081 gactgacttt cttggacagc aaggccatca aaatggacag aattccaaaa actagcaaga
    10141 ctttctgtat gatgaagatg tcaatcctta tccccagaag tggtttatcc caagggataa
    10201 agaagaaaga cagccagatc agcagaacta aaccaatata aaccttggaa tagcgtgtaa
    10261 taaatgattt tagatgtgcc ataagccatc tcctaggatt gtatatcctt tacatattgt
    10321 atatgtccat tttcgacaac taatacatct gtacaaaggt ctgaaatatc ttccgatatg
    10381 tgcgacgtca ggataaccag accattttct tttttcaggt aagataaaat gacctgtttg
    10441 gttaacctca cactcttctc atccaaagca ttcataggtt catcgagaaa gagtatagaa
    10501 ggagaggaaa taaaggcttg aatcaaggcc attttttgct ttgttcctaa ggataaatga
    10561 cggtattcaa cgtcttcaaa ttcctgtaaa tcatagtatt gaatccaata ggcaattctt
    10621 ttttccgtaa ctttagatga aaaatacctt aacagttcca aattttctct cagggataaa
    10681 tgagataaaa actcgacttt ttcaattaaa attcctgcat cctgaatata atgatttttt
    10741 atcccgtaga ctttcccgtc ttgaagaact tttcctttgt caagcttaat ataaccagca
    10801 agtatcttta aaagaaccgt cttgccagat ccattatcac ctttaagtcc ataaattttt
    10861 cctgattgaa acaccaaatt caactgattt aaaataggtc ttttcccata aattttctgt
    10921 ccatttataa tttcaagtct catcgtttat cctttcttaa atttctcttc caagcgacta
    10981 acaatcaaca taactaataa gcttaagagt agtaaataca ctgttacaat atgatccatc
    11041 atccattctt gtcccacaaa tgctaaaaaa tagagaaaaa aggaaaaacc agcgcccgca
    11101 ctaatcagtg ctatcagata agacagtagt aagtacaaaa tcattaactg agcaagtaaa
    11161 aagaaaagtg cgactgtttt gaaatgaatt cctagcggaa gtaaaccaag gaaccagaca
    11221 agtaaatctt gagtaaaaaa tttagaaatg acaaccaatc gtttccttgt ataatcgaaa
    11281 aaggagcttg attgatagcg aatcaagtga tggtaatggt ctgccaaatc actttcaagt
    11341 aaaatgacag agagccatcc taccatgaaa agcgaaaggt ttaacaagag tttggggatt
    11401 tctaaattcg cttcagaata gtcattatag gaaaaaattg aaatcaattc tctcttgaaa
    11461 aagataacag catcctgctt ttgaaccgct gttgacaata gatagtttat caaaatcatc
    11521 atcagaacta tacgaagtag taaaacaatc tttctcttta gcaaaatatt gttaaaattc
    11581 ttaaacatta tcttcatatt ttttaagtac catataaggg acaacataaa ggatataagg
    11641 agtaaagagt ttcatcaaac ttacatcccc atagctagct tgcatcaaac tcgtcatagg
    11701 aaccatatag taaggcaagg cactatagag taatatagaa ccaagccaaa gaaacatcaa
    11761 ataggcgatt gccgaacgag tcacattccc cacataatca actatttgta aaaaatagat
    11821 tgcattgatg aaaataccga ttagtaggac acaagtaaag aacaaatagc tttttatctc
    11881 tccatctagg agtctttgta aaccacttcc atcagaaaat agagaattcc atccaagagg
    11941 agaaaaagtc ccaaggaaat aggttataat tgcaattatc agcacagtca ctaaatatat
    12001 caaacaaggg atacttgcaa cttgcaaagt caactttctt tttaaccctt gatagctcac
    12061 ttctcttcca atactcaatc gtaaaacttt atttttcagc tcaatatatt gaaaacctag
    12121 caaaacaatg atgattggaa taataaatga ttgataaaaa cctagattga actggagaat
    12181 acgaaaatct aatgctgatt ttaataagtc atttttaatt gcttgtccat cgaatttagc
    12241 tagatcgggc gcatgaatat caaaaaagaa ataagtcatc aaaacatcac gataaaccaa
    12301 agcaagaaaa agcaaagtaa gtaagacata aacaaattta tgccttgcca aagttaagag
    12361 aggaatcttc ataagccacc tcctacaaat agaaagtaat ataatactca atgaaaatca
    12421 aagagcaaac taggaaacta gccgcaagtt gctcaaagca ctgctttgag gttgtagata
    12481 agactgacga agtcagttac atatataatc caaggcgacg ttgacgtggt ttgaagagat
    12541 tttcgaagag tataaatcaa tgccttccgc ctttagactt ccctagttcc cgtctcaagt
    12601 caagcatttt tttgaaaaag acataggcta accagtcact agcaaaagcg ataagtagta
    12661 ataatagcca aataccccat aacttgatat ctgtcaaatt tctcaagacg atattgaaaa
    12721 acagaactga aaaaactgtc caagcaaggc taaaaagagc atagagggtg atgtaaatcc
    12781 agtaaaaata gtaaaaaatt gggaaaaatt tactttctct attatccttg tcaatccagc
    12841 tgaaaaagta aaaacagggt aataagatta tcattttaaa caagtgttcc atcacaagcc
    12901 tcctttttta aattgttttc tttagtttcg ttctattaaa tgttattacc aatataaacc
    12961 ttggaatagc gtgtaataaa tgattttaga ggtaccataa gccacctcct acaaatagaa
    13021 accgatataa attaatgcct tccaccctta gacttcccta gttcctgtct caagcgaaac
    13081 atttctttga aacaggaata agttaaccaa ttcataccaa tagctagcag aataaaaaga
    13141 aaccaaatgc cccataactt gatatctgtc acatttctca agacggtatt gaaaaacaga
    13201 actgaaacaa ctgtccaagc aaggctaaaa agagaataga aggggatgta aaaccagtaa
    13261 aaataataaa aaattggaaa aaacttacta tttctgttgg ccttctcaat ccagttatca
    13321 aaataaaagt acggtgctaa aagtaagaat ttaaacaaat gttccatcac cgacatcacc
    13381 ctccttcttt tgatagcgtt ttctattatt ttattatatc aaaaaaatcc ggaactgtca
    13441 ttccagattc tactttttta tttgcgtttt cttgcgatga gatgaatcgg tgttccctca
    13501 aaaacaaagg ccttgcggat ttgattttcc aagaaacgca ggtaagaaaa gtgcatgagt
    13561 tcttcttcat tgacaaagat gacaaaggtt ggtggtttgg ttgccacttg ggtcgcatag
    13621 aaaatcttga gacgttttcc tttgtctgtc ggtgttgggt tgatggcaat ggcatccatg
    13681 atgacatcgt tcaagacagc tgatggaata cgtgtatttt gactttcgct gatttgctta
    13741 atcatctcag gaagtttgtg gagacgttgc ttggttaaag ctgatacaaa gataatcggt
    13801 gcgtaaggca ggtattggaa ctgctcacgg atatcttctt cccagttttt catagtgtgg
    13861 ttatcttttt caagcgtatc ccacttgttg accacgataa tcatcccttt accagcttca
    13921 tgggcaaatc ctgcgatacg cttgtcgtac tcacgaatgc cttcttccgc attgatgacc
    13981 atcaagacca catctgaacg gtcaatagca cgcatggcac gcataacaga gtatttctca
    14041 gtattttcat aaaccttacc agacttacgc ataccagccg tatcaatcat ggtaaactct
    14101 tgaccatctg tatctgtaaa gtgggtatca atggcatcac gagttgttcc agcaacagga
    14161 ctagcaataa cacggtcttc tcccaagata gcattgatca agcttgattt tccaacgtta
    14221 ggacgaccaa tcaagctaaa cttaatgaca tctggatttt cttcctcata tttatttgga
    14281 agattttcta cgatcgcatc tagtacatcc cctgtaccga ttccatggac agatgagata
    14341 ggcaatggtt cacccaaacc gagagcatag aaatcataga tatcatttct catctcaggg
    14401 ttgtccacct tgttgactgc gaggataact ggtttgtggg tcttataaag cttacgagct
    14461 acgtattcgt ctgcatcagt aattccttcc ttaccagaca cgacaaaaac gataacatct
    14521 gcttcttcca tggcaatttc tgcctggtgc ttgatttgtt ccatgaaagg agcatcgaca
    14581 tcatcaattc ctcctgtatc aatcatgcta aaagaacgat tgagccactc acccgttgca
    14641 taaatacggt cacgtgtcac tccttcgaca tcttctacaa tggagattcg ctcaccagcg
    14701 atccgattaa atagggttga tttcccaaca ttgggacgtc ctacaatggc aatagttggt
    14761 agggccataa tttctcactt tctacaataa tttcttttgt tcaagatttt ttctagttga
    14821 gcttggttca gcttgaccaa actgttctgc taggcgctga ctccagcttg tggtcgcacg
    14881 cgccccagca tagtcagcct gaacacggtc ataagcttgg attgcctcag ttgactgttc
    14941 ttggtattct tcctcaaaga caacattctc tagtggcagt ctcggtttca tatcatgatg
    15001 ttgatttggc acacccagtg ccatcccaaa gacagaatag gtgtagtcag gtaggttaaa
    15061 gagctctgcc acttcttcag acttgtatcg aaccaaaccg ataatcacac caccatagcc
    15121 caagctttca gctgccaaca aggcgttttg tccagcaaga gctgcatcga ccgaactaat
    15181 caagagacct tccacacctt ggggttggaa ggtgtcggta tgaagtcggg ctcccttttc
    15241 tgctcggttc aaatctccga caaagagaag gaaaacagca gactggcgaa tggcttcttg
    15301 aggtaccaat tcatacaagg catctttctt ctcttgactt cgtaccacaa tcacagagta
    15361 ggattggaaa ttcttccaag atgatgccat ctgggctgct gtcaaaatct catttaagtc
    15421 tacttgggga atttcttgct ctttaaacct gcgcactgaa gtatgagcct tcatcaattt
    15481 aatggtttct gtcatcgacg gtttactcct tctaaacgag tctcctcagc caaataacgg
    15541 atgcgttcca tgacccgtct ggcttcccag gtttcgtcat ttccattttt ccctttagcg
    15601 aaatgcttct ccaaatcttc aaagttgaag ttagaggtga aaaaggtcgg taaattttcc
    15661 tgcatccgat attggagaat gacctgcagg atttcatcac gcacccaagc ggtggattgc
    15721 tcggcaccaa tatcatctaa aatcaggact tcagaaaact taatctcatc caccaaggtc
    15781 ttaacattgc catcactgat agcatttttg acatcaatga caaagctagg atagtggagg
    15841 agagttgatg aaacaccacg tttttctgat aaatcatgag ctagggcagc caccatgaaa
    15901 cttttaccca caccaaagtc tccatataag taaagacctt ttcgaatagc tggatattgc
    15961 tccacgaagg ctagtagctt ttcaaaaact ggtaagcgcc ccaaatcatc caagtcaact
    16021 tgagccaaac tagctttctt gagactggct ggtagattga ttaacttgag acggttctta
    16081 atagccgctt ctttttcagc cgcgattagc tcaggagttt cttcatatga aacatctgca
    16141 tagccatgat tcttaaccaa aatcggcttg tagcctttgg caatataatc cgtatcccca
    16201 cggagaaact tgtcacgctc ggtgatgtac tgattaaact tggagatact gcgatttaat
    16261 tcctctggag tcaaggattc ttgctggata aaagccgcaa catcagggtc cttcatgatt
    16321 ttctggacca aatcttgata ataaaaacgg ctgggttgac gtttgagtac gtctccgaca
    16381 ctttccatct aatctcctcc tttttctaat cgagctaata gttcttgctt cttacgttct
    16441 agttccagac gagtttcctc gctggtttca ttcttatatt caggattact ccatttagga
    16501 acattggttt ttactggggc agtctgattc tgtttttgtg tttttgcttt ctgtcctcgc
    16561 tcacggatgc gcaagactgc cttttctgcc gaatgaatct tttgataggc atagtcattg
    16621 gctaccttca tggcatattt ctcattgata tttgccgaat cgaccttatt aaaggtcaat
    16681 aagagaatga tattgatgac ttcgtccagc aagcccaagc cagccatctg ttgcaagagt
    16741 tctctttctg tttgggtaat ggttcccttg cgtgtttgct tgatttctgc caagaactgc
    16801 agggcagttt tacttttggc ttctttgata atggttgttt ccttaagact aaagtcagag
    16861 gaaactggtt tttgagcaat tttttcacgc atgcgtttgg ttgaaataac ctgagaaaca
    16921 actgttgact tggccaattg ataggtttca aaccaagtcc atttcttctc ctcggcaata
    16981 gcaaagaggt ttaagacatc ggactgctca tccgcaaaac gaagtccatc tcgagccatc
    17041 agctggcgaa aatgttccaa gtcaaaatca ttggccactt tcttcttgag accaaggtct
    17101 tcttgactgc ctagttctgc caattctgga aagacttgat tgagtgagac aggtatttct
    17161 tcaccatcag cactttcaac tttcaaatcc tccacagcag agtcgccaat ctttttctct
    17221 aagagtctgc gataaacagg atgctccaag aagtcttgac tagatagagg agcatggagg
    17281 gctagctgat aaacatcccc cttttgatag agggtcaaga gattaaaagc agataagatt
    17341 ttcaatgatt ttatcagtct atccatccca aagttgagat gattgaggat acttgaaaaa
    17401 agatattcct ttctaccatt atcccaaaaa ctgattgtat aaagataaag gctcagtgcc
    17461 tcctgaccga taatcgggag gtagcactgt accagagatg aggtatcttg cgacacccga
    17521 ttattcttta gataagaaaa acggtcaatt ggcttcattt atctttcctt tttcttttta
    17581 gaggactggg tgatttgttg gagcaagctc tctaactcac tgacatcctt aaaactacga
    17641 tagacactag caaaacgtac ataggtaatc tcgtccaatt cagccaactc ctccatgacg
    17701 agtgaaccaa tgtcctcact ttgaatttca ttttcatttc gaccacggag tttctgttcg
    17761 atacgattga ctaccatgtt gatttcatca cttgacacag gacgtttctg ggctgagcgg
    17821 ataatcccat taaagatttt atctctggag aattgttccc gtgtgccatc ttttttaaca
    17881 accactaagg ttctttcttc tactcgttcg taggttgtaa aacggtgttg gcattcgtcg
    17941 cactcacgtc ttctacgaat ggtgttccct tcttctgctt ggcgactatc gataacactt
    18001 gacttggtag ccccacattt tggacaacgc atcctttccc tccttatcgt tttcttttca
    18061 ttataccatt ttttaagcga ttcccaaaac aattcttatt tttgcttgac aagtttttcg
    18121 ttttgttgta ttatttaatt aagacaaaag gataaaagaa aggagaccaa gatgtcctgg
    18181 acatttgaca acaaaaaacc catctattta cagattatgg agaaaatcaa gcttcagatt
    18241 gtttcccata cactggaacc caatcaacaa cttccaaccg tgagggagct agctagcgag
    18301 gctggtgtca atcccaatac catccaaaga gccttatcag accttgaacg agaaggattt
    18361 gtctacagca agcgaacaac tggacgattt gtgactaagg ataaggagct aatcgcccag
    18421 tcacgcaaac aattatcaga agaagaattg gaacacttcg tttcctccat gacccatttt
    18481 ggctatgaaa aagaagaact accaggcgta gtcagtgatt atattaaagg agtttaagcc
    18541 tatgtcatta ctagcatttg aaaatgtatc caaatcatat ggagcaacac cagcccttga
    18601 aaatgtttct cttgacattc cagctggaaa aattgtcggc cttcttgggc caaacggctc
    18661 aggaaaaaca accctgatta aactaattaa tggcctctta caaccagatc aaggacgtgt
    18721 cctcatcaac gacatggacc caagcccagc aaccaaggcc gttgtagctt atttgcctga
    18781 tacgacctat ctcaatgagc aaatgaaggt caaagaagcc ctaacctact tcaagacctt
    18841 ctataaagat ttcaatcttg aacgcgccca tcatctactt gcagacctgg gcattgatga
    18901 aaatagtcgt ctcaagaaac tatcaaaagg aaacaaagaa aaggttcaac tgattttggt
    18961 tatgagccgt gatgctcgtc tctatgtttt ggacgaaccc attggtgggg tggatccagc
    19021 agcccgtgct tatatcctca ataccattat caacaactac tcaccaactt ctaccgtttt
    19081 gatttctacc cacttgattt ctgatatcga gccaatcttg gatgaaattg tcttcctaaa
    19141 agacggaaaa gtcgtccgtc aaggaaatgt agatgatatt cgctacgagt caggtgaatc
    19201 cattgaccaa ctcttccgtc aggaatttaa ggcctaagca aaggagatta tttatgtttt
    19261 ggaatttagt tcgctacgaa tttaaaaatg ttaacaagtg gtatttagcc ctctacgcag
    19321 ccgtgctagt cctttctgcc ctcatcggaa tacagacaca aggctttaaa aatctacctt
    19381 accaagaaag tcaggctact atgctacttt ttctagctac agtctttggt ggcttgatgc
    19441 ttacacttgc gatttcaacc attttcttga ttattaaacg cttcaaaggt agtgtctacg
    19501 accgacaagg ctatctgact ttgaccttgc cagtttctga acaccatatc atcacagcca
    19561 aactaatcgg tgcctttatc tggtcattga ttagcaccgc tgtattggct ctaagtgctg
    19621 ttattattct ggctttaaca gctccagaat ggattcctct ttcttatgtg attacatttg
    19681 tagaaacaca tctccctcag atctttctta caggtatatc cttcctacta aatactattt
    19741 caggaatcct ctgcatctac ctggctattt ccattggaca gcttttcaat gaataccgta
    19801 cagcactcgc tgttgcagtc tacattggta tccaaatcgt cattggattt attgaacttt
    19861 tcttcaatct tagttctaat ttctatgtca attcactggt aggactcaat gaccatttct
    19921 atatgggagc aggtatagcc attgttgaag aactcatatt catagctatc ttttatctcg
    19981 gaacctacta catcttgaga aataaggtta atttgcttta aataattttt acctagatat
    20041 gtaacatact catagaacaa aagagaccag gcaaaaagtc tttacaatta gaaaacgcat
    20101 agtatcaagt gttaaaaatc cttgatatta tgcgtttttt gtgagctgac ttatttcctt
    20161 tcactatatc gcaaaatgaa ataagaacgg aacgatggga ttttggaatt caaatcaatt
    20221 tataagaatg ttttagaagt aatattatcc tattccagat tcagttcact atacaattga
    20281 gttttcaagc aacctgttta cataatgtgt acataattag gttcgtgatt ccaccctttt
    20341 cacctttaaa aacctcgctt tcgcaaggct cttctattta taagataagg cacgtttaaa
    20401 ggttttccaa atccctaaat catccgtttg aagaacgaga ctagcataca tgcgtccgat
    20461 aaatcctgtt gctaccaccg caaaaatcac tgtaatagca agtgaaatcc atgcttctgc
    20521 tccccccgca tagtcattaa tcgttcgaaa cggcataaag aaggtcgaaa taaagggaat
    20581 ataagaacca atcttcaaga ggagattgtc accagctgca cctagagctg tcactccaaa
    20641 aaaaccaccc ataatcaaaa tcatcaaagg cgacaaggct ttccctgagt cctcaggacg
    20701 agaaaccata gatcctagga aggctgccaa gactacgtac atgaaaagac tgatcaaaat
    20761 aaagagcaag gtattcagtg agatagcatc tcccaagtga tccaaaatac cagactgagc
    20821 caagaatggc aaatctttaa agagcaaaac ggcagccaga ccacctacaa catagatccc
    20881 aatatgcgtt aaaatcacta gaaacagagc catcatccgc gcatagaaat agtgacttgc
    20941 ccttatgcta gaaaaaacga cttccataat tttggtgcct ttttcactgg caacttcctg
    21001 agctgttaca cccgcatagg caatcagaat catataaaga aagaatccta aggcacctgc
    21061 tgcaattgtt tgaataaact ttttattttc cttgacttca tcaatctttt ctgtgaattg
    21121 aattgtctgc gctaagcgtt tttcctgctc ttgagacaag gaagcagttg aacgattaag
    21181 ctgattttgc agttcattga gtgtacctgt aacctcaaat ttaattccat tttcaagcga
    21241 tgtttcgcca tgataaactg cctttagaac actatcttct tgatcaatgg tcaaataacc
    21301 ttttaatttt tcttctttaa ttgcttcttt ggcacttgct tcgtctttat agtcgaagtt
    21361 aacaccattt acattcttca gtccttctgc tacagatggc actgttgtca ctactgccac
    21421 tttattattt ttagccatag aagaaccttg gatatgccca attcctacag agattcctaa
    21481 aaagaggaac ggcgaaatca ccataaagaa gaaactccat gacttgacat gtcgaagata
    21541 ggtttccttg attacaaccc acatatttct catacttcca ctcctgattc tagtttaaag
    21601 atttcatcga tagttggcgc ttgttggtca aatgttgcga tatattgacc ttgagtcaag
    21661 attgggaaga gttcccttcc agcgctctca tccttcaaaa tcaatttcca actgccttgt
    21721 ttggtcaagc tcacctgttt gacatgagga agattttcca attcttcctt gcttcgttca
    21781 cttgaaacaa agagacgcgt tttcccgtat tgattgcgga catcctgaac tggtccgtgc
    21841 aagaccacac ggccatctcg gatcatcaga atatcgtcac aaagttcctc aacattggtc
    21901 atgacatggt cagaaaagat aatggttgct ccgcgctctt tttcctgaaa aatgacttgt
    21961 ttgagcaatt ctgtattaac tgggtccaat ccactaaaag gctcatccaa gataatcagg
    22021 tctggttcat gaatcagagt aataatgagc tgaatcttct gctgatttcc ttttgacaga
    22081 ctcttgattt tatctgtcag ctttcctttc acttccaacc tcttcatcca ttgagggagt
    22141 ttttctttga cttctttggc atccatgcct tttagagtcg ccaagtagcg aacttgttca
    22201 agaactgtca atttaggcat gagactgcgt tcttcaggca gataaccaat ccgagcatag
    22261 gtctcctgac gaatatcctg accatccaga ccgatttctc cctgatattc taggaatttc
    22321 aaaatactat ggaaaatcgt tgtttttcca gcaccatttt ttccgactag tcccaaaata
    22381 cgacctggtc gcgcttgaaa gtcaatacca aacaaaactt gcttggatcc aaaacttttc
    22441 tctagacttc ttacttctag catctttcac ctccgaaatt tcttgcactc attatactcc
    22501 tttttgatag cctttacaat gttttttgtc catttttaga agactattgc tgtgtaaaat
    22561 atggcctgga gcacttttat actcaatgaa aagcaaagag caaactagaa agctagccgt
    22621 aggctgctca aagtacagct ttgaggttgc agataaaact gacgaagtca gctcaaaaca
    22681 ctgttttgag gttgtggata gaactgacga agtcagtaac tatatctacg gcaaggcgaa
    22741 gctgacgtgg tttgaagaga ttttcgaaga gtattagtga taaatccact atacagtagc
    22801 aaacttaatt tataccttcc gctcctcaac tgtctatttt taatcctgaa ttgttatttg
    22861 agtaactcct ttttcctcat aaagttttct tcctctaaaa cttctggaaa aaggcgaata
    22921 gtttcagaca acatttttat aagaaacaag ttcatctgtc atttcaagaa ggagtaatcc
    22981 tttatctact aatggacgga acagaattca accgcttgtc ccatatgttt tctaaggatt
    23041 atatagtaaa atgaaataaa aacaggacaa attgatcagg acagtcaaat cgatttctaa
    23101 caatgtttta gaagtagatg tgtactattc tagtttcaat atattatatc tattatgcac
    23161 acccctatag gatctaataa aaatcacaac aggttcattc gtaaatggtt acctaagcct
    23221 aagggaacta agaaaacgac taccaaggaa gtcgcattca tcgaaaagtg gattaacaac
    23281 tatcctaaaa aatgcttgaa ctacaagtcc cccagagaag acttctggat gactaac
//
LOCUS       DAVGJJ010000025        23023 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000025 DAVGJJ010000000
VERSION     DAVGJJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.025"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(341..1189)
                     /gene="dprA"
                     /locus_tag="VVE35_000892"
     CDS             complement(341..1189)
                     /gene="dprA"
                     /locus_tag="VVE35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU3061111.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1275..1964)
                     /locus_tag="VVE35_000893"
     CDS             complement(1275..1964)
                     /locus_tag="VVE35_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU3061112.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNVFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1976..2854)
                     /locus_tag="VVE35_000894"
     CDS             complement(1976..2854)
                     /locus_tag="VVE35_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU3061113.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2844..3713)
                     /gene="licA"
                     /locus_tag="VVE35_000895"
     CDS             complement(2844..3713)
                     /gene="licA"
                     /locus_tag="VVE35_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU3061114.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3730..4752)
                     /locus_tag="VVE35_000896"
     CDS             complement(3730..4752)
                     /locus_tag="VVE35_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU3061115.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4757..5464)
                     /locus_tag="VVE35_000897"
     CDS             complement(4757..5464)
                     /locus_tag="VVE35_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU3061116.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5800..7287
                     /gene="tacF"
                     /locus_tag="VVE35_000898"
     CDS             5800..7287
                     /gene="tacF"
                     /locus_tag="VVE35_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU3061117.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFTGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7297..8100
                     /locus_tag="VVE35_000899"
     CDS             7297..8100
                     /locus_tag="VVE35_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3061118.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8102..8911
                     /locus_tag="VVE35_000900"
     CDS             8102..8911
                     /locus_tag="VVE35_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU3061119.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9186..12362)
                     /gene="carB"
                     /locus_tag="VVE35_000901"
     CDS             complement(9186..12362)
                     /gene="carB"
                     /locus_tag="VVE35_000901"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU3061120.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12675..13754)
                     /gene="carA"
                     /locus_tag="VVE35_000902"
     CDS             complement(12675..13754)
                     /gene="carA"
                     /locus_tag="VVE35_000902"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU3061121.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13804..14727)
                     /locus_tag="VVE35_000903"
     CDS             complement(13804..14727)
                     /locus_tag="VVE35_000903"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU3061122.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14746..15267)
                     /gene="pyrR"
                     /locus_tag="VVE35_000904"
     CDS             complement(14746..15267)
                     /gene="pyrR"
                     /locus_tag="VVE35_000904"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU3061123.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15478..16107)
                     /gene="nth"
                     /locus_tag="VVE35_000905"
     CDS             complement(15478..16107)
                     /gene="nth"
                     /locus_tag="VVE35_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU3061124.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPDLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16107..16649)
                     /locus_tag="VVE35_000906"
     CDS             complement(16107..16649)
                     /locus_tag="VVE35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU3061125.1"
                     /translation="MKLNIQEIRKQSEGLSFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            17374..18933
                     /locus_tag="VVE35_000907"
     CDS             17374..18933
                     /locus_tag="VVE35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3061126.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGSLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(19082..19981)
                     /gene="htpX"
                     /locus_tag="VVE35_000908"
     CDS             complement(19082..19981)
                     /gene="htpX"
                     /locus_tag="VVE35_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU3061127.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19983..20543)
                     /locus_tag="VVE35_000909"
     CDS             complement(19983..20543)
                     /locus_tag="VVE35_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU3061128.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            20637..21350
                     /gene="rsmG"
                     /locus_tag="VVE35_000910"
     CDS             20637..21350
                     /gene="rsmG"
                     /locus_tag="VVE35_000910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU3061129.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFGLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            21653..22936
                     /locus_tag="VVE35_000911"
     CDS             21653..22936
                     /locus_tag="VVE35_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU3061130.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
BASE COUNT         6949 a         4883 c         4263 g         6928 t
ORIGIN      
        1 cttatttcaa ttcactatac tttcccttat actcaatgaa aatcaaagcg caaactagga
       61 agctagccac aggttgctca aagcactgct ttgaggttgt agataagact gacgaagtca
      121 gctcaaaata ctgttttgag gttgcagatg gaagctgacg tagtttgaag agattttcga
      181 agagtattat atgaaaaaat cctctactct actaatccac ttagaaaaaa cgcatattat
      241 caagtgttaa aaacttgcta atatgcgttt tattatggaa atgttgggtt cattttctct
      301 taaaattgat tttttcttag aattctagct taggtcattt ttaaaattca aattccgcaa
      361 gaacatcttg cccactggtg accaattttg ctccttcttg aatcaaatga tggcaaccgt
      421 ctgatagtcc atctaaaatg ctaccaggaa tagcaaagac atcgcgtcct tcttccattg
      481 ctcgctcaca ggtaatgaga ctacctgaac gcatcttagc ctctgctaca atcacaccac
      541 gacaaagtcc agcaatgatg cgattacggg caggaaaatg aaatttcaga ggttgttcgc
      601 caggtccata ttcacttaga gccagatggt cattgccgat gtagtcttgc aagcgtttgt
      661 tggctttagg ataaaacaca tccagtcctg ttccaatcac tgcaatggtt tttccgccat
      721 tctgaagagc tgccatatga gctgctgtgt caatgccctt ggccagacca ctgacaataa
      781 ccagttcatt ttccaagcct tgaatgactt tttcaactga cttagctccc tgtttgctac
      841 aagcacgact gcccacgacc gctaccttcg ggaatttcag gaggtcaaga tttcccttgt
      901 aaaataaaag tacaggcgca tcatatattt cactcaaatc ccaaggataa cagtcatcta
      961 aaatagagaa agatggaaat ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa
     1021 aataacgttc cataaaaacg gctggattac ggcaacctga gatatctgca atatcaccca
     1081 acaaaagctc ctgatcaaca ttttcaccgt attctagcac tttcaaaatc tgttgattgg
     1141 tcaaacctga tttttttaac ttatagattt catagtttgt gattttcata aataactcca
     1201 tttctttttc tactcatcta tctattcgta aaaaatcaaa aaacatccga ctattttgtc
     1261 agatgttgga atctttaatt ttcgttttta agaatttctt ctaatttacg atagtcttgg
     1321 acactatcga tctcataaat gctattgcct tctaattctt caacatagac atctagctct
     1381 ttgatattat ccttaaccat attgtcccag tagagatcaa caaattcacc acttgcataa
     1441 gccttgtcga taaagctgac aatcttttct gcagttggag catcccagaa ggatacacca
     1501 ctaaggatgc gacctgcctt gctatcaaca ataatgtctt gaaccttgta gtcatctcca
     1561 tagaccaaga accattcgtt ggtacaatct tcacgataaa cactaaaata agtcgaacgt
     1621 gtcaaatcat tgcggaacac atttttaaag agataattgt cagcatcaat aacatagctg
     1681 ttggccaatt cttcttttac aagatagaga gagtaaaagt tattgtagtc agcgtattta
     1741 tcattgaaaa cgaggcgaac accgtatttt tcttttaaat aatcgaattg ttctttaaga
     1801 taaccaacga tgatgatgat gtcattgatt cctttttctt tgagaaactc aatttggtat
     1861 tcaatcaaag gtttttgatt aacctgaacc aaggctttag gggtattttc agtcatagga
     1921 cgcaagcgag ttcccaatcc cgctgctaag ataatggctt tcacacgaat ctcctttatt
     1981 ccttaataat aatataaact ccagcaatga cgacaagtga cgtcataatg gtcagcatat
     2041 ctaatgatgt tcccaagaaa agagctgcga acaagacagt ccatactaca tagctcacgt
     2101 tcaagcctgt agccttggct ggttgcaagc gattgatagc gatataataa gccaaatagg
     2161 aaatcatatc aaaggctaca aagacaatca tgagacctag caattgtcca ttggctactg
     2221 caacaaatga ctgatgagag aagagcacaa tcacaagata ggacaagaac gaagttactt
     2281 gacggattaa gagggcttcg atttcactca attcactttc catggcaaaa gagctaagaa
     2341 cactctcact tccccatgca atagcacaaa ccaaagcaca aagaatccca atgtagaaag
     2401 aattaacctg ttcaacctta taggtctggg caataatccc tccaataatc aagacaatcc
     2461 caaatacagt atttttcgaa atcttgtgct tcaaaaagaa gaaagccaat agaactgaaa
     2521 tcgcggggta aatagccgat acagatgaag ctaaagaact tccgatatac ttaactgcat
     2581 aaagattggc ctgcataccg atagggcctg ctagcaaggc tccgatgata acactgacat
     2641 tgcgaatatt taagaaaatt gagaggcgaa ctttcccttc ttttaccaag agaaaagcta
     2701 gtaagataaa gatgctcaaa aaatcatgag ttgcagccac cacaaagggt gacaaatctg
     2761 taaaaatcga aaagatataa gcactaaccg ttagacccaa gccccagaaa atacctgaga
     2821 gaaggccaaa aggaactccg tttttacttt tcatctgaac ctccataaga agccaaacct
     2881 ttaacagctc tttggtaacg attcacacca tagtcaccaa aatctgcacc ttgctcttcc
     2941 ttatagacag tccatagact ccaaatagta tcttgtaaaa ttttataaat agcaatcttt
     3001 tcatgagaaa ccggtgtttg gtcactctca tagtgagata agaaagtttc ttcctcttgg
     3061 gaagtgaatt cagactctaa aaagagggca gccaaatccc acattggatc attcattgat
     3121 gaatattccc agtcaatcaa ataaagtcgt ccttgaggtg attcgataaa gttttcaggc
     3181 accaaatcga tatgacaaga ttttctgtca acacctaagt cagccagtct tttctctaag
     3241 gagaagactg catttctaac agattcatag ttggcataag gaatttgttc ttcaatcaag
     3301 gattcgtatt ttttgatttc ttcaaaagga gcaaattctc ctcttaattc cttagcagac
     3361 gtatgaatag tttgtaatat tggagtaatt ttgtcgaact tggtcttgat tgacgttgaa
     3421 tcaagcgtaa tcgcagattc gatatactca tttactttga taccagcttc aatatcaaaa
     3481 agataatttt ttacatctaa gcctaaatcc tttagtagtt caagattgta cttttcatct
     3541 tgtcgattga taagcttttc tgtcccttta ccaaagaatt taacaatgta ttgcttattt
     3601 gttgttttgg ccaaatagtt ttgattggtc attccaccca gttgttcaac actgaggact
     3661 tcctcttctt gactaagtaa ggaagaaatt ttttctttaa tgattttctc cacaattaac
     3721 ctccagtact tatacttccc acttaaacac tgttttaaag gctgtgttta aatcggttgc
     3781 aaagacacga tgaatatctt taatttctct tacaggttct tctagataaa ggatattttt
     3841 aagacgattg gcaaatttct tgacttccat catttggata gcattttcaa aatcaatgcg
     3901 accagaacga gatgacccaa ccaaaatcaa gcccttttct aaggcatcgc gagtattgag
     3961 attgacttta tattcgctaa ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg
     4021 aatcaagtca ttaatagctg gtccagtacc atcaccacca caacattcaa aagcatggtc
     4081 aaaggccaaa tcttcaggaa tattatccgt aatatagcat tctttggcaa atgagaagag
     4141 ttccaacttt tcccaatgac gaccaataac cacaatctct gcttctggca aagtatagtt
     4201 gataatattg gcaaccacaa aagctaaact tccatctcca ataacggcga tccgctcccg
     4261 cttgctatga gcaagagtca atagacgatt catagcgtgc atgcccacac tgacaaactc
     4321 tgtaatggct gcaaccgtat cttcaatagc atcataagcc accacacgat ctttagggag
     4381 agaaacaaac tctctcataa agccatcaaa tccactagac aagaaatggg tccctgtcat
     4441 gtagttttca tagaattctt catcactctg cataggagac tgattgggaa tcatgacaac
     4501 tttttgacca acctcgtagg ttccggtcgg gtcagaaatg acggttccac atgactcgtg
     4561 aatcattgcc attggaagct ttttattcaa aatcttggga tcacgttttc cctgatagta
     4621 acgctgatcc gcatgacaga cagccatgta gttgggacgg ataaggatat gattctcttg
     4681 gtcaatagcc tcttcctgat atttgacatt gataaactta ggcttagtta gttgataaat
     4741 ttgattaatc attttactag tctttctcaa tcatactttt tgcaatcttc aaatctgtta
     4801 cggttgtaat cttcagattt gagtattcac ctttggccaa ggccacatct tttcctttga
     4861 tcacaaagat tttacatgca tctgtcaaga tttccttctc ttcatcagaa agagatccat
     4921 aaaggtccat gaagtccttg caacggaatg tttgaggtgt ttgtccttga taaaggtgag
     4981 cacgatttgg aatatctgta ataaattgac cattggtact ttcaacgata gtatcaaccg
     5041 cttctaccac tgtgtccact gcgtcatgat tttgggcaag ttggatattg tcctgaatca
     5101 tgcgaagtgt aataaatgga cgaacagaat cgtgggtaac aacgatatcc tctggagtaa
     5161 gcggacgata agcatcaatg gcttcaatga ttttcttaat acttgtattg cggtcagcac
     5221 caccctttgt aatgatgata cgttccttat aaagaggaag atatttatct acaagatctt
     5281 ctgcatgaga aacccagtct ccatgaacac caactacaat tttttcaata cttggctcca
     5341 agacaaattt ttcaattgta tgaatcaaaa taggtcgatc acctagctct aaaaattgtt
     5401 ttggcaagtt actgatcccc atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa
     5461 tcatctattt tctcctttgt cttactaaaa aaacttattc tctctattat accacaatct
     5521 agtcctatca taaaagaaat actggtcctc cccttctaga ataggagaat tatgcagttc
     5581 tattattcaa agaaactagt ccatttctag gaatgactag gaattctgta gcaattacta
     5641 ataaatggct gcgaaatttt agagtccctc agaataatat actttcctta aagaaaactt
     5701 aaaacttaag atttgaaact tcaaagtgat tacttgactt actgcccttc atttcttatt
     5761 agctaacttt atgatataat gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat
     5821 gctctatctt acatgggaat tcgtgtcttg aatattattt ttcccatcct aactggaacc
     5881 tatgtcgcgc gtgtcttgga ccgaactgac tatggttact tcaactcagt cgacactatt
     5941 ttgtcatttt tcttgccctt tgcaacttat ggtgtctata actacggttt aagggctatc
     6001 agtaatgtca aggataacaa aaaagatctt aacagaacct tttctagtct tttttatttg
     6061 tgcatcgctt gtacgatttt gaccactgct gtctatatcc tagcctatcc tctcttcttt
     6121 attgataatc caatcgtcaa aaaggtctac cttgttatgg ggattcaact cattgcccag
     6181 attttttcaa ttgaatgggt caatgaagct ctggaaaatt acagttttct cttttacaaa
     6241 actgccttca tccgtatcct gatgctggtc tctattttct tatttgttaa aaatgaacac
     6301 gatattgttg tctatacact tgtgatgagt ttatcgacgc tgattaacta cctgattagt
     6361 tatttttgga ttaaaagaga catcaaactt gttaagattc acctaagtga ttttaaacca
     6421 ctctttctcc ctctgacagc catgttagtc tttgccaatg ccaatatgct cttcactttt
     6481 ttagatcgcc tcttcctcgt taaaacaggg attgatgtca acgttagtta ctataccata
     6541 gctcagcgaa ttgtgaccgt tatagctggg gttgtaacag gtgcaattgg agtgagtgtg
     6601 cctcgtctca gttactatct ggggaaagga gacaaagaag cctatgtttc tctggttaat
     6661 agaggtagtc gaatctttaa cttctttatc attccactga gttttggact catggtttta
     6721 ggaccaaatg ccatcctact ttacggtagt gaaaaatata tcggaggcgg catcttgacc
     6781 tctctcttcg cttttcgtac gattatcctg gccttagata ccattcttgg ttcccaaatt
     6841 ctctttacca atggctatga aaaacgtatc acagtctata cagtctttac tgggctactc
     6901 aatttgggct tgaatagtct cctctttttc aaccatatcg tggctcccga atactactta
     6961 ctgacaacta tgttatcaga gacttctcta cttgttttct atatcatttt catccataga
     7021 aaacaactca tccacttggg acatatcttt agctatactg ttcgatactc tctcttttca
     7081 ctttcctttg tagcaattta tttcctgatt aatttcgtgt atcctgtgga tatggtcatt
     7141 aatttgccat ttttgattaa tactggtttg attgtcttgt tatcagctat ctcttatatt
     7201 agtctacttg tcttcacaaa agatagcatt ttctatgaat ttttaaacca tgtcctagcc
     7261 ttaaaaaata aattcaaaaa atcataggag tttaaaatga aacaactaac cattgaagat
     7321 gccaaacaaa ttgaattaga aattttggat tatattgata ctctctgtaa aaagcacaat
     7381 atcaactata ttattaacta cggtactctg attggggcgg ttcgacatga gggctttatc
     7441 ccttgggacg acgatattga tctgtccatg cctagagaag actaccaacg atttattaac
     7501 atttttcaaa aggaaaaaag caagtataag ctcctatcct tagaaactga taagaactac
     7561 tttaacaact ttatcaagat aaccgacagt acgactaaaa ttattgatac tcgaaataca
     7621 aaaacctatg agtctggtat ctttatcgat attttcccta tagatcgctt tgatgatcct
     7681 aaggtcattg atacttgtta taaactggaa agcttcaaac tgctgtcttt cagtaaacat
     7741 aaaaatattg tctataagga tagcctttta aaagattgga tacgaacagc cttttggttg
     7801 ctccttcgac cggtttctcc tcgttatttt gcaaataaaa tcgagaaaga aattcaaaaa
     7861 tatagtcgtg aaaatgggca gtatatggct tttattcctt caaaatttaa ggaaaaggaa
     7921 gtcttcccaa gtggtacctt tgataaaaca atcgatttaa cctttgagaa tttaagcctt
     7981 cctgcacctg aaaaatttga tactattttg acacaatttt atggagatta tatgacccta
     8041 ccaccagaag aaaaacgctt ctacagtcat gaatttcacg cttataaatt ggaggattag
     8101 gatgcaatat ttagaaaaaa aagaaattaa agaaattcaa ctagccctgc tggactatat
     8161 tgatgagact tgtaagaaac atgatattcc ttattttctc agttatggaa ccatgcttgg
     8221 agccatccgc cacaaaggta tgattccttg ggatgatgat attgatattt ccctttatcg
     8281 tgaggattat gagcgtttac tgaagattat tgaagaagaa aatcaccctc gctacaaggt
     8341 tctttcctac gatacatctt cttggtactt ccataatttc gcatcgattt tggacacttc
     8401 tactgttata gaagaccatg ttaagtacaa gcgtcatgat accagccttt tcatcgatgt
     8461 cttcccaatt gatcgattta cagacttgag cattgtcgac aagagctata agtatgtggc
     8521 tcttcgtcaa ctagcttata tcaaaaaatc acgagcagtt cacggtgata gcaaactaaa
     8581 agattttctt agattatgta gctggtacgc tctccgattt gtcaatcctc gctactttta
     8641 caagaaaatt gatcaactag tcaaaaatgc tgtaaccaac actcctcaat atgaaggagg
     8701 agttgggatc ggtaaggaag ggatgaaaga aatcttccca gttgatacct ttaaagaact
     8761 gattttaact gagtttgagg gccgtatgtt gcctgttccc aaaaaatatg accaattttt
     8821 aacccagatg tatggcgatt atatgacacc accatcaaaa gaaatgcaag agtggtatag
     8881 tcatagcatt aaagcttatc gcaaaaactg attgaggggg attatacaaa ctactaagat
     8941 agaggttatt caaaaacata attttagtag aaaatgaaat aaatattccc acaataaaac
     9001 gcatcatatc aaggtttttg aaaaaccttg atatgatgcg ttttataatt ttaaagactt
     9061 ttttctgtag tagattgaaa taagatgcga acaaatcaat tagaaaattc aaattaattt
     9121 ctagaaatat tttagtattt ctgtgtactg ttctaaattc agtctgctat atcttatttt
     9181 tctatttaaa tcgcttctgt aacaaagcta cgactttcaa gtaccttaag catggcatta
     9241 gctgtatcta gcgctgtgaa gaggggcacc ccgtgttcaa tggctgaacg acgaatttgc
     9301 tcaccatctt cgtcagcagt tcgttttgtt cctactgtgt taatgatagc ttgaattctt
     9361 cctttgcgta caaaacttgg gatatcctta tcgtcatcac caatcttacc aacaggttgg
     9421 gcttgcaagc catgactagc aaagaaggct gctgtccctt ctgtcgcaag gattccataa
     9481 ccaatgtttt ggaaacgacg agccaagttc aaggcttctt ctttggcatc atcagcgatg
     9541 gtaaagacga cattaccaaa agttggcaag tgtagataag aagcttcaaa ggctttatag
     9601 agagcttttt ccaaagtagc atcagaaccc ataacttcac ctgttgactt catttcagga
     9661 ccgagcaagc tgtctacctt agctagtttg gtaaaggaga agacaggtgc cttgatatga
     9721 acacgggtgc tttcagggta aagtccattt tggtagccaa gttctgataa actttgacca
     9781 agaatgagct tggtcgctac ttgagccata ggaatattgg ttaccttaga taggaatgga
     9841 acggtacggc tggcacgtgg attgacctca ataacgtaga ctttttcatc cttgataaca
     9901 aactggatgt tcatcattcc aaggcagtga agaccgattg ctaagcgttt ggtgtagtct
     9961 gcgatggtct cctgaacctt ttgcgacaag gtttgtggtg ggtaaacagc cattgagtca
    10021 cctgagtgga caccagcacg ttcgatatgc tccatgatac caggaatgag tacatcttta
    10081 ccatctgaaa tggcatcaac ttcgcactct tgcccaacga tataagagtc gacaagaact
    10141 gggtgatctg gactagcctt aacagcagtt cgcatgtaag aacgaaggtc ttcttcgttt
    10201 tcaacgattt ccatggcacg tccaccaagt acataagatg ggcggacaag aactgggaag
    10261 ccaatcttac gagctgcaag agctgcttct tcttcattgg tagccgtttg tcctggtggc
    10321 tgtggaatat ccaattcttt gagagcttgc tcgaagaggt cacggtcttc ggcacgatct
    10381 aggtcagcaa cctgtgtacc aaggatggtc acacctgctt ttgccaatgg ctccgcaagg
    10441 ttgatggctg tttgaccacc aaactgaacg ataactccct ttggttgttc caagtcaatg
    10501 acgttcataa catcttcgaa tgtcaatggc tcaaagtaaa gcttatctga tacagagaaa
    10561 tctgttgaaa cggtctctgg gtttgagttc atgatgatag cttcataacc agctgcctgg
    10621 atagccttaa cagagtgaac ggttgcgtag tcaaactcaa ccccttgacc gatacggatt
    10681 ggacctgaac ctaggacaag tacagattct ttatcagatc tgatagattc attttcccaa
    10741 ccataggttg aatagaaata tggcgtttca gagtcgaact ctgccgcaca agtatctacc
    10801 atcttataaa ctggaacaat cttgttttcc aagcgaagtt ggcgaacttg atcatcagtc
    10861 gttccccaga gttcagcaat cttacggtct gagaaaccat taagtttggc tgttttcaaa
    10921 acttctagat cttgtggatg agcacccaat tcttgctcaa tttcaaagat atgcaagagt
    10981 ttatcaagat agaagatatc aatttttgta agctctgcaa tttcttcagg tgtgtagcca
    11041 cgacgaatgg cttctgatac gtagaagaga cggtcatctt gggctttgac aaccttttca
    11101 atcaaggcat catcagaaac tgctgcaagt tcaggtattt cattgtggtg caccccaatt
    11161 tcaagggagc ggcaggcctt gagaagagat tcctcgatgt tacgaccgat tgccatgact
    11221 tctccagtcg ccttcatttg tgtaccgaga cggcgttcac ccttttcaaa cttgtcaaat
    11281 gggaaacgtg gaatcttagc aactacgtag tcaagggctg gttcaaacat ggcataggtt
    11341 gaacctgtaa ctgggtttat aacctcatcc aaggtcaaac ctactgcaat cttggcagcc
    11401 aacttagcaa tcggatatcc tgtcgcttta gaagcaaggg ctgacgaacg tgatacacga
    11461 gggtttactt cgataacata atacttgaag ctgttaggat caagagctag ctgaacatta
    11521 catccacctt caatcttgag ggcgcgaata atgctcaagc tcgcatcacg aagcatttgg
    11581 ttttcatagt ctgacatggt ttgcgcaggg gcaaatacaa tggaatcccc tgtgtgaatc
    11641 ccaactgggt caaagttttc catgttacaa acaaccaagg cattgtcagc tgagtcacgc
    11701 atcacttcgt attcaatttc cttgaaaccg gcaatcgaac gctcaatcaa acattgggta
    11761 acaggtgaca atttcaaacc attttcagtg atttcacgca attctttttc gttggcacac
    11821 ataccaccac cagtaccacc aagggtaaag gctggacgaa cgatgactgg gtagccaatt
    11881 gtcgctgcaa aggcaactgc ttcttctact gtgttaacaa tttcagattc tggaatgggt
    11941 tgctcaagct cttccatcaa ttgtttaaag aggtcacggt cctccgcttg gtcaatggca
    12001 gacaatttag taccgagaag ctctacacca agctcatcta agatgccatt tttagacaat
    12061 tccatggcca tgttgagacc tgtctgacca ccgagtgttg gtagcaaggc atctggacgt
    12121 tccttacgaa gaatacgtgt cacaaactca agtgtaatcg gttcaatgta aaccttatca
    12181 gcaatttcct tgtccgtcat gatggttgca ggatttgagt taaccaaaac aacctcataa
    12241 ccttcctctt tcaacgacaa gcaagcctgg gtcccagcgt aatcaaactc agcagcctga
    12301 ccaataataa tcggaccaga accaatcacc ataatttttt gaatatcagt acgtttaggc
    12361 atatataaga tattaagggc gtcaagcgga caaagctaaa ataggagtta tgacgaagaa
    12421 ctgtcagttc taggaataac tatcttttta gcaccgtccg tagcccgtat tcagttcagc
    12481 aaatacggag cacccttctc ctttctattc gtagcctctc agggcgacat taaataagat
    12541 acaaaggacg aatagaaagc gattgaattt taggaaatca aggaaggatt gacaatccaa
    12601 gttggtttct ctaaattctg agctttccgt ccatgttcag ttacataaat tctccgacga
    12661 gcttttactc attcttagtt tgattgttta aaaacttcca tcatctcgat aaactcgtca
    12721 aataggtagc tagcgtcgtg tggcccagga gctgcatctg ggtggtattg aacagagaaa
    12781 gcaggttggt atctgtggcg cacaccttcc actgacttgt cattgatttc ttcgtgggta
    12841 ataatcaagt gctctggcaa atcctcgcgg ctgactgcat aaccatggtt ctggctggtg
    12901 aagtctactc gtcctgttgc gatttcacgt accgcatggt tgaatccacg gtggccaaac
    12961 ttcatcttat aggtcttagc cccgtttgcc attgcaaaga gttggtgtcc catacaaata
    13021 ccaaagattg gaatttttcc ttgtacaccg cgaatcatgt cgagtgcttg tggaacgtct
    13081 tctgggttac ctggaccatt tgacaacata actccatcag gattgagatg gagaatttct
    13141 tcagccgttg tcgaataagg aacaactgtc acgttacagt tgcgtttaga aagttcacgt
    13201 aggattgagt gcttgagacc aaagtccact agcaccacgc tcaaaccaac tcctggagct
    13261 ggataagacg ttttagtaga aacctgtttg atattgtctg tcggtaaaac tgttgcttgg
    13321 agctggtccg tcacatggtc catactgtcc ccaacatggg tcaaggttgc acgcatagta
    13381 ccatgcttac ggataatctt ggtaagagca cgcgtatcaa ttcctgaaat ccctggaatt
    13441 ttcttggctt tcaaaaattc atccaaggtc atttggttgc gccagttgct agctctacgc
    13501 gcttcttcaa aaacaacgac tcccttacaa gttggaataa tggattcata atcatcacga
    13561 ttaataccat aatttcctac caaaggataa gtaaaggtca agatttgtcc attataagac
    13621 tggtctgtaa tggattcttg gtagccggtc atccctgtat taaagacgat ttcgcctgtt
    13681 acatcaatat ctgctccgaa ggccttgcct tcaaaaactg tgccatcttc taatactaga
    13741 attctttttg tcatattttc acctctcgtg gacgctcact ggcgtctttt aacgtcttgt
    13801 gttttagttg gcgtttctac tcgctagtac ggattctaag attgccattc gaacaaagac
    13861 accattggtc atttgttgga caatccgtga ttttggtgct tcaaccaagt ggtctgctat
    13921 ttctacatca cgattgattg gagctgggtg catgaggatt gctgtttctt tcaaacgatc
    13981 gtaacgttct tgagtcaagc catgttgggc atgatagtct tcttttgaaa atacagctcc
    14041 actatcatgg cgttcgtgtt gcacacggag aaacatcatg acatcaacct gatcaatgat
    14101 ttcatcaatg gttacaaact ttccatagtc tgcaaactct tgacttctcc attcctcagg
    14161 tccagcgaaa aagagttcag ctcccaagcg tttcaaaatc tgcatattgg atttggcaac
    14221 gcgtgagtgg tccaagtcac ctgcaatagc aactttaaga ccctcaaagt ggccaaattc
    14281 ctcataaatg gtcatcaaat caagcaagct ctggctaggg tgttggcccg aaccatctcc
    14341 accattgatg atggaagtcg taatcgttgg actagcaatc aattctctat agtagtcgac
    14401 ctctggatgg cgaatcacac agacatccac tcctaaagca gacagagtca aaatggtgtc
    14461 ataaagtgtc tcacccttat taaccgaact agtcttcaca tcaaagtcaa gtcgttccaa
    14521 tccaagttta atctctgcga cttcaaagga cttatgtgtc cgtgtagaat cctcaaagaa
    14581 gagattggaa acaatcggat ggtcttcata gggaagctgg gctccatttt taaactcaat
    14641 tcctcgcttg atcaatttca ttacttgatc gacagtgagg tcttccatgg acaccacatg
    14701 gttcaatgct tgttgatttt ctgacatggc tactccttta actttctaag cttcttcagt
    14761 aatcagaact ctgtcttggt catcaagttc tgtcatctct acgatgattt cttcagaacg
    14821 actggttggg atattttttc caacgtaatc tggacggatt ggcaattctc tatgtccacg
    14881 atcgactaga actgctaaac tcacacgcgc aggacgacca tgaccgacaa tattatcaat
    14941 agcagcacgg atggtacgac ctgtatagag cacatcatcc accaagataa cttcgcggtc
    15001 tgtcacatcg acagaaacca aagaagtatc ttctccactt ttaacatcat cacggaaagg
    15061 tttagtatcc aattccacaa caggaactga aagattttct aactgcttca aacgttcttg
    15121 gattcggtgg gcaataaaga caccacgagt tttaatacca gccaagacga tcttattcaa
    15181 atctttgttg cgttcgataa tctcataagt aatacgcgta atcgctcgtt tgacggtcaa
    15241 ttcgtctaca acttcttttg ttttcatgac aaacctccaa aaagaaaagt ctccttaaac
    15301 aaggagactt gaaatttata gccaagcgag ccctgctgca cacagtatag acttcaccct
    15361 tctactttat cgcgctcctt gcctgcctca cgggacaggt ttaaaggaat atttagttat
    15421 catttactat agcacaaagc atgcttaaaa tcaagcaaaa agtttcaatg tagcatctta
    15481 caaattgcta aaatcatata attgtgggta ctggtcacac tctggatttt ttggatggca
    15541 aatggctctt ccaaaataaa tcatggcctg atgggcagct aaccactgct caggcggcaa
    15601 gatatccatg acccgctttt ccacctcaag tggcgtcgct gattttttga caatatcgtg
    15661 gtgtttgcaa atacgctcca catgagtatc cactgcaaag gctggaatcc caaatcctac
    15721 actcatgaca acattggctg tcttgcgacc aacacctgcc aaactctcca attcttcacg
    15781 tgtctgaggg acttgaccat caaaatcgtc tagtaactgt tgggcacatt ttttaaggaa
    15841 tttagcttta ttccgataca atcccaagcg agaaatatgt gaagcaatct cactctctgt
    15901 cgctacagac atagcttggg gtgttggaaa ggcaacaaag agatctggtg tggccttatt
    15961 taccgctgca tctgtcgtct gggctgataa catgaccgca accaggagtt caaaatgatt
    16021 ggtaaaatca agactaggct tggcatctgg gaagagggca atgatttctt ctagcacctt
    16081 tcgtgctcgt ttttttgaca agaccattat tcatctccgt caaatagtcc ttgtaagcca
    16141 gcaaaaggac tgttttcttc tttctttact gctttttgag cttggtattc ttcctctgtc
    16201 atgatttgcc agtcatttcc tgagataaat ccttgaccag cttcttcttc agccgtcaag
    16261 accttgatag gaatgtttag caggatattg tctgatacac tctcagcaag gtcaagctcc
    16321 ccattttcga tgggcaagac caagtcatca tctaaaactt cttgatctag ctggttagtt
    16381 gcgccttcca tgaaaacttc cgtgactgga taagattcaa ctaactcaac tggctccata
    16441 ctgcgactcg aagcaagaac aatggtataa gatagttgat aatctaagaa atacatacgg
    16501 tcttcatatt gtactttccc aactgcaagg atatctttta catctaaaat ttcttgatta
    16561 cgtgcacgca ggtcatcaac taaatctaac gtttgttcaa agctcaaacc ttcagactgc
    16621 ttacgaattt cttgaatatt taatttcata cttcctccat aaagatttac tctcttgatt
    16681 ataccatgaa aaggctacaa atcagcacac caaactttgt aattaaaatt cgaaatttta
    16741 acatatttac tatgacagtt ttatttttta gtgctatact atagggaaag agtacatcag
    16801 agcaaggagg atgctcacac ggaagacaag aaactcattc aactcctatc caagttaaat
    16861 aaaagctacc aaaactgtaa acagggtacg gcagatgata ttcgactaca agagctgcta
    16921 aacactacta tgcaagagct caaaaaacag aaaagttgaa caacagtatc ttaattgatc
    16981 ttgagaaatt ttaccaaccc accagtcctc tgattggact gggtagccta aaactaaaca
    17041 atcaagaacg cactgcttgg cgaaactatg ataaaatcca ttacgagcat gtcaaacacg
    17101 tactaagtct ctatggacct gtttttggat tttagagcat agaatttcca gttttctgtt
    17161 gacaaaattt ccttaaaggt atagtataaa gatactaata ctcggaggta acggagacat
    17221 gaacaactaa gtctatcaaa taaagaacct ttatttagta gatcttgttt ttgtctcttt
    17281 ttgtgtgctc ttttatgctc tttttctggc atgttaatag agtttttttg acatagactt
    17341 tgggctctac taggtaaagt agagcttttt gttatgcact atgaacattc tagaaaggga
    17401 aatcatatga taaaaatcaa tcatctaacc atcacacaaa acaaagattt acgagatctt
    17461 gtatctgacc taaccatgac catccaagac ggggaaaagg ttgctattat tggtgaagaa
    17521 ggaaatggca aatcaacctt acttaaaatt ttaatggggg aagctttgtc tgatttcact
    17581 atcaagggaa acatccaatc tgactatcag tcactggcct acattcctca aaaacttccc
    17641 gaggagctga aaaagaaaac tttacacgac tacttctttt tagattctat tgatttagac
    17701 tacagtatcc tctatcgttt ggcggaggaa ttgcattttg atagcaatcg tttcgcaagt
    17761 gaccaagaga ttggcagtct atcagggggc gaagctttga aaattcagct tatccatgag
    17821 ttagccaaac cctttgagat tctattttta gatgaacctt caaatgacct agaccttgag
    17881 acagttgatt ggttaaaagg ccaaattcaa aagaccaggc aaaccgttat tttcatttcc
    17941 catgatgaag actttctttc tgaaacggca gacactattg ttcacttgcg actggtcaaa
    18001 caccgtaaag aagcggaaac gctagtagag catttagact atgatagcta tagtgagcag
    18061 agaaaggcta attttgccaa acaaagtcag caagctgcta acaaccaaag agcctacgat
    18121 aaaaccatgg aaaaacatcg gagagttaag caaaatgtag aaactgcgct tcgagctacc
    18181 aaagatagta ctgccggtcg cctattggct aaaaagatga aaactgtcct ctcacaagaa
    18241 aaacgctacg aaaagacagc tcagtccatg actcaaaagc cacttgaaga ggaacaaatc
    18301 caacttttct tttcagacat ccaaccatta ccagcttcta aagtcttagt ccaactggaa
    18361 aaagaaaatt tgtccattga cgaccgagtt ttggttcaaa aactacaact aactgtccgt
    18421 gggcaagaaa aaatcggtat tatcgggcca aatggtgttg ggaaatcaac tctgttagcc
    18481 aaattaaagc aacttctgaa tgataaaaga gagatttccc ttggttttat gccacaagat
    18541 taccacaaaa aactgcaatt ggatttatca ccaatagcct acctcagcaa aactggggaa
    18601 aaagaggaac tacagaaaat ccaatctcac ctagctagtc tcaatttcag ttatccagaa
    18661 atgcagcatc aaattcgctc cttatctggc ggacaacagg gaaaactcct gcttttggat
    18721 ttagtcctgc gcaaaccaaa ctttctcctg ctggatgaac ccacacgaaa cttttctccc
    18781 acttctcaac cccaaatcag aaaactcttt gccacttatc ctggcggtct catcactgtt
    18841 tcgcatgacc gtcgtttctt aaaagaagtc tgctcgatca tctatcgcat gacagaacac
    18901 ggtttgaagc tagttaattt agaagattta taaatttgca acatagcaga taggtggggc
    18961 aaaaacacaa ttttaggaga aaaagaagta aatcttccca tagtaaaacg cataatatca
    19021 agttttcaca cctgatatta tgcgtttttc agattttaaa gacttttttc cagccttcat
    19081 tttacatctg ttttaaacgt tcaatccgtt ctgagatagg tgggtgggta taaaagagtt
    19141 tttggaaccc cccacctttc ttgggatcat tgatataaag agcactgcta gcatcatcga
    19201 cgtggcgact cataggtttg ctattgtcca acttatctag ggcattaatc attccctggg
    19261 gattgcgagt cagctcgaca ctagatgcat ctgccagaaa ttccctctga cgagaaatag
    19321 cgagctgaac caaggttgca gcgagaggtg ccagtacaat agctagtagg gaaaccacta
    19381 gcataatgat ttcaagacca tttccatctc ggtcatcatc acttcgtctg cgacctgctc
    19441 caccccacca catcatacga cctgccatac tagaaagcat ggtgatagca ctagcaagag
    19501 caactgcaat agtcgaaata cggatatcat aattacgaat atgactgact tcatgtccca
    19561 taacagcttc tagttcttca cgattcatga tagctagtag acctgaagtc gcagcaacag
    19621 ccgcattttg aggattagaa cctgtcgcaa aggcatttaa ggctggatca tcaatgatga
    19681 aaacacgggg cataggaatc tgagcgacca gagccatatc ttccactaca tggtagaggt
    19741 ctggtgccgt ttgctcatcc acctcacgcg ctccattcat ggacatgaca atctctgtcg
    19801 attgaaaaat catagacaaa gcgtagataa agccgataat cagtgcgata accaaaccac
    19861 caagtccaga tcttataaag agataaccaa ccgcataacc aacaagagct aagagtagga
    19921 aaaataccag caacaaaatc caggtttttc gtttattgct tgcaatttga tcaaacaaca
    19981 tcttagtcac ctaaaccgct aaaatcaact ttaggaaccg acttttcctc ttcaggtgtt
    20041 tgaaggaaat ctgccgcttt aaatccaaac attccagcga taatattgct cgggaaagtt
    20101 tctaatttta cattgtagtt gctgacaaca ctgttataga gttgacgaga gtaagaaatt
    20161 ttattttctg tatttgtcaa ctcctcttgc aatttaacaa agttagcact agctttcaaa
    20221 tctggatagc tttctgcaac tgcaaaaata cctgaaacct gacgagtgag ggcatcactg
    20281 gctttcatag cttctgctgg tgaagtcgct gccgccactt ggttacgtag ttctgccacc
    20341 ttttcaaggg tagaaccttc atatttggca taacctttta cagtctcaat caagtttggc
    20401 aagagatcat tgcgacgttt caactgaaca tcaatctgac tccaagcctc cttggtttgc
    20461 atacgatttt taaccaaacc gttatagcta acaatcacaa aaataacaac aagagctaaa
    20521 actccaagaa taatccaagt cataatataa gtcctttctg cttttagatt agtaccagta
    20581 tatcaaattt tctatgattg tggtaaaata agatgatact aaagaaggaa ataactatga
    20641 aaccaaaaac attttacaac ttgcttgccg agcagaatct tccactttcg gaccagcaaa
    20701 aagaacaatt tgaacgttat tttgggctct tggtcgagtg gaatgagaag attaatttga
    20761 cggcgattac ggacaaggaa gaagtttatc tcaaacattt ttacgattcg attgcaccca
    20821 ttcttcaagg tttgattccc aatgaaacta tcaaacttct tgatatcggg gctggggcag
    20881 gatttcctag tctaccaatg aaaattctct atccggagtt agatgtgacc attattgatt
    20941 cactcaataa gcgcatcaac ttcctacaac tcttggctca agaactggat ttgaacggag
    21001 ttcatttcta ccacggacgt gccgaagatt ttgcccaaga caagaacttc cgtgctcaat
    21061 atgattttgt aacagctcgt gcggttgccc gtatgcaggt cctatctgaa ttgactattc
    21121 cctaccttaa ggttggtgga aaactattgg cactcaaggc cagcaatgcg cctgaggagt
    21181 tgttagaagc taagaatgcc ctcaatctcc tttttagtaa agtcgaagac aatctcagct
    21241 acgccctacc aaatagagat ccgcgttaca tcacagtggt agaaaagaaa aaagaaacac
    21301 caaataaata tccacgtaag gctggtatgc caaataaacg cccgctttaa atttttaagt
    21361 aaaaaaatat ttacaaaatc aacctcgctc tcttattttt aggctcggga aaaaatgatt
    21421 tacaaaatca gcctcgctct tttatttcta ggctcgggaa aaaatgattt acaaaatcat
    21481 ttttttctgc tatactatcc taagcaaagg tttttaatgt catcccgtga ggtgacgaag
    21541 acgcagaaat atttaaaact ctttaaaatc taaattttaa agaagtctta ctctgagggc
    21601 ctattgctgt aaaataatgg gctctttttt gatgcccaaa agtgaggttt atatgaaaca
    21661 agaatcaact gttgatttgt tattagacgt tgaccaacgt ccttcggctg gaaaaggaat
    21721 tctccttagc ttccaacacg ttttcgccat gtttggtgcg accatcttgg taccattgat
    21781 tttgggaatg cctgtatctg ttgccctttt tgcttcaggt gttggaacac tcatctacat
    21841 gattgctact ggttttaaag ttccagttta tctaggttct tcatttgcct ttatcacagc
    21901 tatgtcactg gctatgaaag aaatgggggg tgatgtatct gctgcccaaa caggggttat
    21961 cttgactggt ttagtctatg tccttgttgc taccagcatc cgatttgtag gaacaaaatg
    22021 gattgataaa ctcttgccac caatcattat cggtcctatg atcatcgtta tcggtcttgg
    22081 acttgcaggt tcagctgtta ccaatgcagg tcttgtagca gacggaaatt ggaaaaatgc
    22141 tctggtagcc gttgttactt tcctaattgc tgcctttatc aatacaaaag gaaaaggctt
    22201 cctacgaatc attccattcc tctttgccat tatcggtggt tacctcttcg cactaactct
    22261 tggcttggtt gactttacac cagttcttaa agccaactgg ttcgaaattc ctggtttcta
    22321 cttgccattt agcacaggtg gtgcctttaa agagtacaat ctttactttg gtccagaagc
    22381 catcgctatc ttgccaatcg ctatcgtaac aatttctgaa catatcggag accatactgt
    22441 tttgggtcaa atctgtggcc gtcaattctt aaaagaacca ggtcttcacc gtactcttct
    22501 tggtgacggt atcgcaactt ctgtttctgc cttccttggt ggaccagcca atacaactta
    22561 cggagaaaat acaggggtta tcggtatgac tcgtatcgct tctgtctcag ttatccgtaa
    22621 cgctgccttc atcgcgattg ccctcagctt ccttggtaaa ttcactgcct tgatttcaac
    22681 tattccaaac gctgtacttg gtggtatgtc aatccttctc tatggggtta tcgccagcaa
    22741 tggtttgaaa gtcttgatta aagaacgtgt tgatttcgct caaatgcgaa acctcatcat
    22801 cgcaagtgct atgttggttc ttggacttgg aggagctatc cttaaacttg gtccagttac
    22861 actttcaggt actgcccttt cagccatgac aggaatcatc ttgaacttga tcttgccata
    22921 cgaaaataaa gactaagagt ctaaatacac ctaatccact cagacagctg agtggatttt
    22981 tcgtatacca taataaaagt gtcttaacaa aattattaaa atc
//
LOCUS       DAVGJJ010000026        22758 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000026 DAVGJJ010000000
VERSION     DAVGJJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.026"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1229
                     /locus_tag="VVE35_000912"
                     /pseudo
     CDS             1..1229
                     /locus_tag="VVE35_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1401..2963)
                     /gene="guaA"
                     /locus_tag="VVE35_000913"
     CDS             complement(1401..2963)
                     /gene="guaA"
                     /locus_tag="VVE35_000913"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU3061131.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3105..3803
                     /locus_tag="VVE35_000914"
     CDS             3105..3803
                     /locus_tag="VVE35_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3061132.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3846..4742)
                     /locus_tag="VVE35_000915"
     CDS             complement(3846..4742)
                     /locus_tag="VVE35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU3061133.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSAFTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4751..5686)
                     /locus_tag="VVE35_000916"
     CDS             complement(4751..5686)
                     /locus_tag="VVE35_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU3061134.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGSVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5683..6408)
                     /locus_tag="VVE35_000917"
     CDS             complement(5683..6408)
                     /locus_tag="VVE35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU3061135.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESIEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6492..7127)
                     /locus_tag="VVE35_000918"
     CDS             complement(6492..7127)
                     /locus_tag="VVE35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU3061136.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKMIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(7129..7956)
                     /locus_tag="VVE35_000919"
     CDS             complement(7129..7956)
                     /locus_tag="VVE35_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3061137.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAKTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7978..8904)
                     /locus_tag="VVE35_000920"
                     /pseudo
     CDS             complement(7978..8904)
                     /locus_tag="VVE35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            9555..10166
                     /locus_tag="VVE35_000921"
     CDS             9555..10166
                     /locus_tag="VVE35_000921"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU3061138.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10211..10939
                     /gene="rlmB"
                     /locus_tag="VVE35_000922"
     CDS             10211..10939
                     /gene="rlmB"
                     /locus_tag="VVE35_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU3061139.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10978..11889)
                     /gene="trxB"
                     /locus_tag="VVE35_000923"
     CDS             complement(10978..11889)
                     /gene="trxB"
                     /locus_tag="VVE35_000923"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU3061140.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRSFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11955..12179)
                     /locus_tag="VVE35_000924"
     CDS             complement(11955..12179)
                     /locus_tag="VVE35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU3061141.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12257..13000)
                     /locus_tag="VVE35_000925"
     CDS             complement(12257..13000)
                     /locus_tag="VVE35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3061142.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13000..13800)
                     /locus_tag="VVE35_000926"
     CDS             complement(13000..13800)
                     /locus_tag="VVE35_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3061143.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDIVQTDVKGDLR"
     gene            complement(13958..14314)
                     /locus_tag="VVE35_000927"
     CDS             complement(13958..14314)
                     /locus_tag="VVE35_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU3061144.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14308..14838)
                     /locus_tag="VVE35_000928"
     CDS             complement(14308..14838)
                     /locus_tag="VVE35_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU3061145.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(14838..15332)
                     /locus_tag="VVE35_000929"
     CDS             complement(14838..15332)
                     /locus_tag="VVE35_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061146.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15467..15913
                     /locus_tag="VVE35_000930"
     CDS             15467..15913
                     /locus_tag="VVE35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU3061147.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15910..16557
                     /locus_tag="VVE35_000931"
     CDS             15910..16557
                     /locus_tag="VVE35_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU3061148.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(16578..17159)
                     /gene="pdxT"
                     /locus_tag="VVE35_000932"
     CDS             complement(16578..17159)
                     /gene="pdxT"
                     /locus_tag="VVE35_000932"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU3061149.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17160..18035)
                     /gene="pdxS"
                     /locus_tag="VVE35_000933"
     CDS             complement(17160..18035)
                     /gene="pdxS"
                     /locus_tag="VVE35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU3061150.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18187..19566)
                     /locus_tag="VVE35_000934"
     CDS             complement(18187..19566)
                     /locus_tag="VVE35_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU3061151.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLKNVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19860..20783
                     /locus_tag="VVE35_000935"
     CDS             19860..20783
                     /locus_tag="VVE35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU3061152.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20843..21448
                     /locus_tag="VVE35_000936"
     CDS             20843..21448
                     /locus_tag="VVE35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3061153.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEETDGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            21467..22711
                     /locus_tag="VVE35_000937"
     CDS             21467..22711
                     /locus_tag="VVE35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU3061154.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
BASE COUNT         6793 a         5129 c         4101 g         6735 t
ORIGIN      
        1 gattcacaca aggaaatcat cgccaaactg gactacgacg ccccatcttg ccctgagtgc
       61 ggaaaccaat tgaagaaata tgactttcaa aaaccttcta aaattcctta tcttgaaacg
      121 actggtatgc ctactagaat tctccttaga aagcgtcgat tcaagtgcta tcattgttct
      181 aaaatgatgg tcgctgaaac ttctatcgtc aagaagaatc accaaatccc tcgtatcatc
      241 aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta tgactgatat tgcccatcag
      301 ctttccatct caacttcaac tgttattcgt aagctcaatg attttcactt taaacatgat
      361 ttttcttgtc ttcctgagat tatgtcttgg gatgagtatg cttttacaaa gggaaagatg
      421 agcttcattg cgcaagattt taacaatctc aatatcatca ctgttcttga gggaagaaca
      481 caagctatca ttcgaaatca ctttcttaaa tatgatagag ccgtccgatg tcaagtgaaa
      541 attattacta tggatatgtt tagtccttac tatgacttgg ctaaacagct tcgctttcaa
      601 atttctaggc tcaggctgaa acagtctccc agactgtttc actcccgaat gctaaaatcg
      661 ttcttgatcg ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt gtgcgtgttc
      721 aaatcatgaa tcagtttcat cgaaaatccc atgaatacaa ggctatcaag cgctactgga
      781 agctcataca acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc
      841 gtatgcattt aaccaataaa gagattttag acaagctttt gagctattca caagacttga
      901 aacaccacta tcagctctat caactcttgc tgtttcactt tcagaataag aaaccggaga
      961 aatttttcgg acttattgag gacaatctta agcaggttca tcctcttttt cagactgtct
     1021 ttaaaacctt tctaaagaac aaagagaaaa tcgtcaacgc ccttcaacta cactattcta
     1081 atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat gcctttggtt
     1141 ttcgaaactt tgaaaacttc aaaaaacgga tttttatcgc tctgaacatc aaaaaagaaa
     1201 ggacgaaatt tgtcctttct cgagcttagc tttttttcaa cccactacag ttgacaaaga
     1261 gccctttttt gtccaaatgt aaaccacttg gaactattat tcaatttatt ctttttagta
     1321 aatgttcccg ttttaatccc agttacagtt tttaatcctc tgtaaccgtt gattttacaa
     1381 agcttttaat ttttttgcga ttactcccac tcaactgttg caggtggttt acttgtaata
     1441 tcgtagacga tacggttaac atgatccact tcatttacga tacgtactga gattttttga
     1501 agtacttccc atggaatttt ggcaaaatca gcagtcatac catcgataga agtgatagca
     1561 cggattgcaa tcgtgtagtc atacgtacga ccgtcaccca taacaccgac tgaacgaacg
     1621 cctgtgttaa cagtgaagta ttgccaaata tcgcggtcaa gtccagcttt agcgatttct
     1681 tcacgaagaa tagcgtctga ttcacgaacg gtttcaagtt tctcttcagt gatttcaccc
     1741 atgacacgga tagcaagtcc tggtcctggg aatggttggc gccatacgat atggtctggc
     1801 ataccaagct ctgtaccaag agcacgaact tcatccttgt aaagagtatt gagtggttca
     1861 atcaattcaa actgcatatc ttctggaaga ccacccacgt tgtggtgtga cttgatagtt
     1921 tgagctgtat ccgtaccaga ctcgataaca tctgtatata aagtaccttg agcaaggaat
     1981 ttcacatctt tgagcttgct tgcttcgtca tcgaatacat agacaaactc gttaccgatg
     2041 attttacgtt tttgttcagg gtcagaaacg ccagcaagtt tgtcaaggaa acgtttagca
     2101 gcgtctgctt tgacaatatt caaaccaaac ttaccaccga gcatgtccat aacttgatca
     2161 gcttcgcctt tacgaagaag accgtggtct acgaagatac agatcaattg atcgccaatc
     2221 gctttttgga gaagaacccc aacgacagat gagtcaacac cacctgatag accaagaagg
     2281 acacgtttat caccgacggt ttcacgaatt tttttgatct gcatgtcaat gaaattatcc
     2341 attgaccagt cacctttagc cttacaaatg ttaagggcaa agttacgaag aatatcattt
     2401 ccgtatacag aatgacgaac ttctgggtgg aattggatac cgtaaatgtg tttatctggg
     2461 ttttcgatgg ctgcgtatgg gcagtcagct gatgtacctg tacgaacaaa gtcagcagga
     2521 atctcagtaa ccgcatcacc atggctcatc aaaacagtct gttcatcagg tgttgattca
     2581 aaaagcgctg atggtgtgtg agttagggtt gattgaccgt attcacgatt tccagcatca
     2641 cctgcaggaa caacttttcc tccaagttta tgggtcaata actgcatacc ataacagatt
     2701 cccaaaattg gaattccgag ttcgaagatt tctgggtcaa tatcaaatga accatcttca
     2761 tatacagaat ttggaccacc tgatagaata attcctacag gattgacttc acgaacttca
     2821 gcagctgaaa ttttatggct ttttagttct gaaaaaacac caatctcacg gatacggcgt
     2881 gaaatcagct ggttgtactg gctaccatag tccaatacga tgattttttc tacatcttgc
     2941 aaatcagttg aaatgttgct catctttttc ctttctcaaa agaaatatct tttctcaata
     3001 ttctatcaca aataagggcg aattaccaac taaacaaggc gaagtttgac tttttctagt
     3061 ttattggggt acaatagaga aaagatagaa atgaagtgaa aaatatgcta cctgcttata
     3121 tgaaaatcca tgatcagata aaaaaggata ttgacgagca ccgttgggct attggtgaga
     3181 ggcttcccag tgaaagagat ttagctgagc agtttgcggt cagtcgcatg accctccgcc
     3241 aagctgtatc tctattagtc gaagaaggcg tcttagagcg ccgtgtagga agcggcactt
     3301 ttgtttccag tactcgagta caagaaaaga tgcgagggac aaccagtttt actgaaattg
     3361 tcaaatccca aggtaaagtt ccctctagcc agctcatttc ctacaaaaaa accattccca
     3421 atgagcaaga agttgccaag ctaggaattt ttccaacgga caatattatc cgaatggagc
     3481 gggtccgtta tgccgaccaa gttcccctag tttatgaagt tgcttctatt cctgaaaaat
     3541 tcattaagga ctttaaaaaa gaagaaatca ccagtcattt cttccaaacc ttgcaaaaat
     3601 atggctatcg tatcggcaaa tctcaacaga ccatctatgc tcgccttgct aaagaaaaga
     3661 ttgcccacta tttggaagtt gaaaaaggac atgctattct tggattgaca caggtttcct
     3721 acctagaaga tggtactgct tttgaatacg taaaaagtca atatgtaggc gaacgctttg
     3781 aattttatct tgaaaataat tagattacca cgcaaaaact ctctgtcacg gacaaagagt
     3841 cttttttatt tttctaagaa taaatctttc aaagaaatca aagttacagc aaacaatatc
     3901 attgccatac caagaaaatc aatgggataa aattgttcat tcattattaa gaaggcaaag
     3961 aaaatcgccg atattggctc aattgaagcc aacaagcttg acttgaccgg tcctatcaga
     4021 ctggctcctt taaggaaagc tgtataggca aagacagtcc cgataaggat aatgcctgca
     4081 aacgcaagga gaaaatcaag actagtcggg atatcggcct gtagaacccc tgtaaaagga
     4141 agagcgacca aacctgctat gaccattccc acaccaatga ccaagctgct cccccacttt
     4201 ttaatcaagg ctatgggtaa aatgatatac agagcataag taaaggcaga aaagagaccc
     4261 cagaacagac cagcaggtgt catggataac tggtccaact gcccatgtgt tgcgatcagg
     4321 aaggttcctc cgatggcgaa tatgatggaa actatctctc ccagtgtcgg tgccacccta
     4381 tccttgatac agctataaat taaaattccg acaggacaaa catactgaag caccgtcgct
     4441 gttcccgcat tggtctcctg aatagcagac agataggcga attggttgag aaaaagacca
     4501 atcagagcaa aaataagaag agacagcaaa ctctttctat cctttaaaaa gaccagtatt
     4561 ttatcctttg cagtagcata agccaagagc atgagaattc caccagcgat taaaagacgc
     4621 aagttggtca agaccagagc cgaaattccg tgtgccatta ggtattggcc actcgttcct
     4681 gacaaccccc aagcaatacc agccacaact gttagtaaag tcccttttaa actatttgac
     4741 atgtctctcc ttactcttct ttgcgaatca agttcatcac ttgattgcgg tctaatatcc
     4801 attgagctct ctcgtccttt tcataggtac gattcgatag gaaaatagtg gcttcttgct
     4861 tctgacgatt ccacatgata aaggtacctg tatagcccgt atggtctagc caatctcctt
     4921 ccaaattcca tgctaaagaa cgttccttgt catccaaagg agaaaaattt tgacttaagt
     4981 ctcttgcaaa atcatctgct aaatagtgtt ctaaaaagat ttgtaaatcc tttatagtcg
     5041 aaaataaacc agcactccca gcatgtctac ccaggagacg agccttggga tcatgcacta
     5101 tgcctgcctc tacacctcta actgttggaa cagcaagctc aactgaccca aacttggttt
     5161 ccgtcattcc ccaaggtttc cagacttgat cctttaaaat cacatccaaa tcttgattaa
     5221 aaattctttc caaaataaag cccaacagca aaaaatggac atccgaataa agaaaggctg
     5281 gctgacttcg tctgttgaga tgaaacatcg cttcctttaa ttcaggggct gttaaaagat
     5341 cacgattagg aataaaagga tcaaggtctg ttgcatgagt caagagctgg cgaatagtga
     5401 tgtctggata atcactctca ggtaaaaaat ctattaccag tctatcaata tctaattgac
     5461 ctatttccca caagaaggta caaactgtgc caaccccaac aaccttgctg acactagcta
     5521 ggtcataaac tagtcctgcc tcagtctgca agccatgctc tgggtcactc tggcctaaat
     5581 agaactctgt ccattgattg tccttaaaat acgcaaaaga ggctccggga taaatccctg
     5641 cctcgatttg ttcttctatt tttttaatga tcttggtcca cttcatactt cttcaaacca
     5701 caattcaaca ttatttctat ctttaccaag gaagaatttt tcagacttag gaataaaata
     5761 ttcaatagac tcaaacttct ggcgaagact tgctatgtct aattcattga ccaagaactt
     5821 gagcatagat aagtcccagg taaccgtatt gtccacagtc aaatcctgcc cctgagctgg
     5881 aataaaatca agggatgccc caatttcaga tgattccaag aaactttcga tatcagttgg
     5941 gagatgtaat tccatagaaa tctcaaattt acttaaagaa attgatgcca aatctgtttg
     6001 aaattcaggc ttttctccta cttctactag acttgctctg tcatcttccg catgaatcaa
     6061 aatcaaatca tcttctggtg agaaaatttc aaaagcgtag ccattttgac ctttatataa
     6121 tcgatgaatc gaatctgttt tagataagat tccttcaatt tctaagggat tttccacctt
     6181 gacaatcaat ctagctagtt tttttcttcc ctctacctta cgagtacgca tactgggagc
     6241 ttcttctaaa accagctttt caagacccgt ttggtcacct agtgacagaa aggccgattc
     6301 ttctaacaag gccttcattc caagggtttc aatataaaat gtttcattta attttctatt
     6361 attagctttt aaagtaggaa taatccgtac aatctgattt acattcataa attcctccgt
     6421 cactttttat tttaaaggat tttagaattt tttacaagat attatgctca aatttaacat
     6481 tatttagcta tttagtaaag atagtctttc aaggcttttg acaaagcctg ataaccagca
     6541 atactgaggt gcagtccatc agttgtatat tcttttttga gttggcctgc ttggtctgtc
     6601 aaacaatcaa atactggcac aaattccacc tgcatatagg cagatgcaag ctcttgatag
     6661 gcttgattcc agttctgaat tttttcattc gagcggatat agactgcctg ctggtactcc
     6721 tctccctcat tgacaggcaa aatggaaagc aatttaatct ctgtcaatgg ataatcgcga
     6781 gcaacggatt gaatgatagc ttcgagatta ttgagagcct cattcacagg aacatccttt
     6841 ccgatatcat ttgtcccaat cagaagaaaa attttatcta ctgctccacc atatagatga
     6901 gcatcaaggt tctctagtaa cagtcctgtc tgatagccac gaattcctcg attgacaatc
     6961 atctttgaag tcccaaatag ctcctgtaga ggataatact cgacaatgga atccccaata
     7021 aaaagaatgt ttggttctac aacagaaatg tgatttaggt gacgatactt ggtttgaatt
     7081 ttttcttgtt cctttaggag ccaattttct aataactgta ctgccacttc attctccttt
     7141 ttctaaccag ttttccaaga cttgataaac tcctccttgg ctgttggctg gtgctagagc
     7201 agtcgccaca gctttggctt tctcatcagc attttccatc gcataggcaa ttccagccat
     7261 ttcaagcatt tcaacatcat tttcactatc accaaaagcc atgatttctt gggatttcaa
     7321 gtcccagcgc ttgagtaatt cctccaagcc ccatgcttta tgaatcccag cttggaggat
     7381 atcaatgcaa ccatagccac tggatacagc tcggacacgg ccatcaaaga gagtattgat
     7441 ttcttccaaa accgaactca aacgttcctc accaacaacc atgctcatct tgagcacacc
     7501 accaaagagg tcagatgtta attcatccac aaattgcatc cgttggtaga atttttcaat
     7561 catttctgga gtcataaaac tttcaagatc tgtaaaaatc gtaccttcct tgacaaaatc
     7621 acccttcatc cccgttacaa caaactggtc ctgacacgct cgacccttga aatgagccaa
     7681 agccttgttg acaatggcgt catcccatgt cttagcctga atcaattcat tgttttcaaa
     7741 aatacgagcg ccattagcaa caaccagaac cactcgatcc accaagggac tcagtagttg
     7801 tctcatgcgg tgaatttcat tgcccgtcgc aatgacaaaa cgaatgcccc tttgatccaa
     7861 ctgatctaaa atcttttcca atcgtgggag atcaagctga cctctagcat ccagcaaggt
     7921 cccatccata tctgtcgcaa ttaccttaat catcatttac tttcctctgg attcaagcta
     7981 ataccacttc aatcccacat gttcattcat ttctttatag gctgtatcaa ttcgttcctt
     8041 agtcgcttca tctaactggt cacgatgact gccatcctct ataaaatctt ccaaaatata
     8101 agtttggtaa tgatacaagg gatatccttc tggtgaaaag gttccctttg gtgttcgatt
     8161 gacccaagta ggatgagctt tagctgttcc gatagttgtt tttccatcct tcttcttgat
     8221 ggtgacatcc atgagaacac cacgttcagt ccacttagca ttctcttcat ctcccataga
     8281 ttcaattcgt tgattggaaa tgaagttccc catttaatag acaatgagct tcttatctcc
     8341 atctttttcc accgtttcag atggttcaac aacgtgagga cgccctccaa agataatatc
     8401 cgctccccaa tcgatcatct tgtgataaag agctttttgt tcttcagttg gttccaatcg
     8461 atactcaaca cccatctgag gcatgataat ggtgatatct gcttccttct ctgcccgttc
     8521 aatttcagcc ttcatcttat cttcgtttaa atctgaaaga taacgattat agtcttcctg
     8581 agaaatatac tgctcaattc cattgaaacc ataggaatag gctaacaatg caaccttgat
     8641 accattcact tccttaatga ccagcggagc ctgaacacgt ggttcgtgcg tataaactcc
     8701 gattggagtg attccagctt tctcaataat atcggccgtt gaaataactc cctcaatttg
     8761 cgaatccaaa atatgattat gagctaaatc cagcacatga taacctgcct ccttaatagc
     8821 atccataact tcaacaggag cattaaagag aagataaccc gctaaataat gatccttatt
     8881 aatggttcct tcaaaatcac caatagctaa atctgctgct tagtcccctt gcttgagcca
     8941 aggagtcaca tactcaaaat tttcatgaaa gtcataggta ccgtcttctt ttttaactga
     9001 aaagaaaagt ccatcgtggt agagcaaatc cccgtgggcc ataattctgg cagttttttc
     9061 ctcttcctac tcctgagact tttgcttagt cccctcttga gaaatagtat ctcgtttttg
     9121 actagtcaag ggattccttg gaagctttca aacaacaaga ccaagcccat tgataaacca
     9181 actgctagca agagtatcgc cacaaatccc ttattgctcc acttgcgata actcctaaaa
     9241 agtttaccaa gcccttcata aaacgaaaag ctaaaccacc ctgatttcga ttttgtcttc
     9301 tttgtatctt cattctccct actttcttat gcaagccttt tctttttatt atatcacaga
     9361 taagtatttc tttcacaatt gaattgaact tcccatctat tttctataaa tcctaaatgc
     9421 cataatgctt tcaattcttg tcattttgtg atatcatgta gaggaaatga actaatccac
     9481 agtggcttat tccaagtata ccacttgggc tttggcagta gctaactgca ctaaacataa
     9541 tataaggaga gaaaatgtct gcaatagaac gtattacaaa agctgctcac ttaattgata
     9601 tgaacgatat tatccgtgaa gggaatccta ctctacgcgc gattgctgag gaagtcactt
     9661 tccccctatc tgaccaggaa atcatcctag gcgaaaagat gatgcaattc cttaaacatt
     9721 cccaagatcc tgtcatggct gaaaaaatgg gactccgcgg tggtgttgga ctggctgctc
     9781 cccagttaga tatctcaaaa cgcattatcg ctgttttggt acctaatatt gttgaagaag
     9841 gcgaaactcc acaggaagcc tacgatttgg aagccattat gtacaatcca aaaatcgtct
     9901 ctcactctgt tcaagatgct gctcttggcg aaggagaagg ttgcctgtct gttgaccgta
     9961 acgtgcctgg ctatgttgtt cgccatgccc gcgttactgt tgactacttt gacaaagatg
    10021 gagaaaaaca ccgtatcaaa ctcaaaggct acaactccat tgttgttcag catgaaattg
    10081 accacattaa cggtatcatg ttttacgatc gcatcaatga aaaagaccca tttgcagtta
    10141 aagatggttt actgattctt gaataaagaa aatcccgttg caagacgggg ttttgtgtta
    10201 taatagaggc atgaaaacaa atgatattgt ctatggtgtc cacgccgtta ccgaagccct
    10261 ccttgcaaat acaggaaaca aactctacct ccaagaagat ctccgaggta agaatgttga
    10321 gaaagtcaag gaactagcta cagaaaagaa ggtgtccatt tcttggacat caaaaaaatc
    10381 tctctctgag atgactgaag gtgctgttca tcaaggtttt gttctacgag tgtctgaatt
    10441 tgcctatagc gagctagatt acatccttgc aaaaacacgc caagaagaaa atccacttct
    10501 attgattcta gatggtctaa ccgatcccca taatctgggt tctatcttgc gaacagccga
    10561 tgcgaccaat gtttcaggtg tcatcattcc caagcaccgt gctgtcggag taactcctgt
    10621 cgttgccaaa acagccacag gtgctattga acacgttcca attgcccgag tgaccaacct
    10681 cagtcaaacc ttagataaac tcaaggatga aggcttctgg acctttggaa cggatatgaa
    10741 cggtactcct tgccacaagt ggaatacaaa agggaaaatc gccctcatca tcggaaatga
    10801 aggaaaaggc atctctagca acatcaaaaa acaggtcgat gaaatgatta ccattccgat
    10861 gaatggacat gttcaaagcc tcaatgccag tgttgctgca gccattctca tgtacgaagt
    10921 tttccgaaat agactataaa aaagtttcca gtcatctgat tggaaacttt tttatgatta
    10981 actatgttct gtgataaact tgtaggcttc ttgaccagcg atagctccat ctccaactgc
    11041 tgttgttact tggcgaaggt ctttcaagcg aacatctcca actgcaaaga taccgtcgac
    11101 tgcagtcttc atgtggctat ctgtcacaat ccagcctgcc tgatcttgga tattcaattc
    11161 tttaacaaaa tcgctaagag ggtccaaacc aacatagata aagacaccac cgaaggcttg
    11221 ctctgtcact tgacctgttt tcacattttc aaatacgact gattctactc ggttttcacc
    11281 cttgatttcc cttactacag aatcccagat aaagctgatt ttttcattcg caaaggagcg
    11341 atcttgtaaa accttttggg cacgaagttg gtcacgacgg tgaacaatgg taacagtctt
    11401 agcaaaacga gtcaagaaga gggcttcttc aacagctgaa tctccaccac caactaccaa
    11461 taaatcttgg tcacggaaga aagcaccatc acacacagca cagtaagaaa caccacgact
    11521 gttcagttct tcttctccag gtactcccaa aggacggtgt ttagaaccag ttgctacgat
    11581 aactgtacgt gtttcatatg tttggtcatc agtcatcact ttcttaaaat caccatggtc
    11641 ttcgacattt tcaacataac cataaatgtg ctcaacacca agattttcaa gtggttcaaa
    11701 catcttttca gccaattcag gtccactaat attagcgtat cctgggtaat tttcgatatc
    11761 agatgtatta ttcatctgac cacctggcag accaccttca atcaaagcta cttttagatt
    11821 gcttcgagca gcatacaagg ccgcagtcat ccctgcaggt ccagcaccga taataatagt
    11881 atcgtacata tagattcctt ctttcttgtt gtaactatct ttattctaac tctttcttgt
    11941 caatcacgca aggattatgc cttgaaaaca agaattaaac tcataaccgc aaaggataat
    12001 actgggacaa ccaagactcc aatcataatc atatcataga gatgggcttg gcgagccttg
    12061 gctgtcttat caactcccga catggctctc agaccaatcc aaatccctaa aaaaatcaga
    12121 acaaggatgg tggtcaagat caaactctcg aaatataaag aaaatagttg cagtagcatg
    12181 atttctctca tttctatctt ttttaaagag taaactcagc tagtccaact aactgagttt
    12241 tcctttatct attatatcaa atataagtcc gtttgtaact agcgaagaat tcttttgtcc
    12301 gctcttcttt aggggtgtgg ataatctcat ctggagttcc agactcgatg attttcccct
    12361 tatctaagaa gagaatctta tccgcaactt gggctacaaa ggacatgtca tgactgacca
    12421 aaatcatggt ctgacctgac ttagcagcat ctgcaataga cttttctact tcaccgacca
    12481 attctgggtc aagggctgaa gttggttcgt ctaagagcaa aacatctggt ttcatagcaa
    12541 gcgcacgcgc tagggcaacc cgttgcttct gtccacctga taaatggcga ggataatggt
    12601 tttcacgatc cgaaagccca accttagcca actcttcctt ggcaatctta gtcgcttctt
    12661 ggtcagataa tttcttgaca acaaccaagc cttctttcac attatcaagt gctgttcggc
    12721 gttcaaacaa attaaactgt tggaaaacca tagacaactt acggcgtagg gcaaggattt
    12781 cttcttgagt gattttagaa aaatcaactg aaaaaccatc aatctgaata gagccactgt
    12841 caggtgtttc aagataattg agactgcgaa gaaaggttga ttttccagct cctgaagaac
    12901 caatcaaggc tacgacttct cctttttgaa tatccaagtt cagatgatcc aagacagtct
    12961 gtcctgaaaa ggatttgctt aaattcgaaa tcttaatcat taacgaaggt ctcctttcac
    13021 atctgtttgc actatatcag gtgcagaaat agccattttt ctctcgatga aacgaccgag
    13081 gctttcaatt ccgatattga ctacccaata aacaagggca acggagatga agcgttcaaa
    13141 atagcgataa tcagctccac ctagaatctg agcttgggca aagacttcca caacacccgc
    13201 actaaaagct agagatgttc ccttggtcaa accgatgagg gaattaatca aggttggagt
    13261 agctaccacc gctgcattag gaataatcac tcgtcgataa acttgcgctc gggtcatacc
    13321 cagactgcgt gccgcctcaa tctcaccagg attaactgag agaatggctg cacgaatggt
    13381 ttcactagca taagctgcct cattaaaggc aaaagcgaca atcgcaaaag ctgcagctgg
    13441 aatcgcattg atattgagac cagttcccca ttgctgattg agggctttca aagccaaagg
    13501 gattccgtag taggtcaaca tgagttgcac caaaatcggt gtccctttta agaaactaac
    13561 aaagaaggcc tgcaagggat ataaaatctt gacacgattg atcttcacaa tggcaaaaag
    13621 aagcgccaaa accaagccaa aaagggcacc accaattgtc aacataattg ttgttggaag
    13681 ttgttggaca attctaggga ttccatcaaa gaccgaacgt aggctaaaca gcttgccatc
    13741 cggaatcaat tgcatcaagt tttggtacca atctgatgct aaaatcgttg taacattcat
    13801 aaaaacatcc tcttctgata atgattcatt ctaccatgct tctgccgtca atgaaattat
    13861 ttgctacggt cagatacaga ctttgtctac ctactagttt atcatacttt gaaacaggga
    13921 agttatatat tttatctatc aaagagatag ataaaaacta tttcagtccc agttcttcat
    13981 aagattttcg ataaccgatt tgcttaacag ttccattttc tactaaaatg ggccgtttta
    14041 acaacatacc gtcacttgct agcaactcag ccgcttcttg gtttgacaaa cttcctacct
    14101 tatcttttag ccctaattca cggtatttga taccactggt gttgaaaaat tgcttcaatt
    14161 caaatcctga ggtttctagc caattcaaaa tgacttcttg gctaggtgtt tcttccacga
    14221 tatggacggc tttatagtcc acacctaatt gatttaattc ttgttttgct tttttacaag
    14281 ttgaacattt ggggtattcg ataaattcta acatgtgctt ctccttctat tgtttctatc
    14341 tttaaaatct actccttcat gctccaagag ccaagctttc ttttccactc ctgcagcata
    14401 gcctgtcaga cgcttgcctg ctcccaacac acgatgacaa ggtactagga tagaccaagg
    14461 attgcgtccc actgctccac caattgcttg agcagaagcc acttgcaggt cttgagcaat
    14521 ttgtccatag gtcactgtct gaccataagg aattccctgt aaataggacc aaactctctt
    14581 ttcaaaattc gttccaattg gcgccaagag caagttggat aaatcctgag gcttgccttt
    14641 aaagtaatca tctaagcaag caataactgg gtctaaaata ggatgactaa caacttcttc
    14701 tatcgtttca tctcctagtc ccctctcaaa atgcttctgc tcctgaaccc aaattccata
    14761 caaataatgg tcatcagcta caagtgatag aataccaatt ggtgaggagt agagtatttt
    14821 tacatacttc ttttgcatta tttctttaat ttttgatagg ccaagcgttc gccatctact
    14881 ggcaccttac caacttgttt aaaaccaagt ttttcaaaaa tatgttgcat aaccttgttt
    14941 tcagcatgcg tatctgagcg aaaatcaaga taatcaaaac cttcaatcaa gccctctaag
    15001 aaggtttgag caactccttt tccctgcaca tctgctgcca cagcaatacg gtgaaagact
    15061 agatactctg actctccagc ttgccagttt ccctcataaa tagcttcata ggcctcctct
    15121 ggactcttgg tcacagcagc ataggctagc agttctccct cttccaaggc tacgtaggct
    15181 tgccctgaga tgatatcatc gataataaca tctgcatttg gatagccatt ttgccactgg
    15241 tcactaccag catctgctaa acattttttg gcatcctcca tcacctgcat gatggcatct
    15301 acttcatttg gaaaagctaa acgaatttcc atcttttttc ctcatttctg actagtttct
    15361 cccatcatag cataatttaa gtatttctac aagcagttaa aacttgactt tgtgtttttg
    15421 ttattttata atctagttat caaaactata taaaaaggag ttgaaaatga aaactacctt
    15481 ttcctaccca aaatgggcag aaattccaaa cattgacctc tatctggacc aggttttgct
    15541 ctatgtcaat caggtctgcg cccctatctc tcctaataaa gacaagggcc taacagcatc
    15601 tatggtcaac aattatgtga aaaatggtta cctgacaaag cctgacaaaa aaaaatacca
    15661 acgccaacag attgcccgtt tgattgctat cacaaccctc aagtctgtat tttctattca
    15721 agaaatagcc cagacactta atactctaca aactcaagca agttcagacc aactctacga
    15781 tgcttttgtg gactacatga accaaggaat tgacccagct aaccctatta tccaaaccag
    15841 ctgccaaacc gttaaactct atcatcaaac tctagactta atcgatcata ctcaagagga
    15901 ggtaatccaa tgaacactag tcttaaactc agcaaacaac tcagttttgg agaggagatt
    15961 gctaatagcg tgacccatgc tgtgggtgca gtcatcatgc ttatcttgct gcctatttca
    16021 tccatctata gttatgaagc acacggattt ttatcctcta tcggcgtttc cattttcgtc
    16081 atcagtctct ttctcatgtt cctatcatcc accatttatc actctatggc ctatggttcg
    16141 acccacaaat atgttttgcg aatcattgac cattctatga tttacgttgc cattgccggc
    16201 tcatacacgc ccgttgtctt gaccttgatg aataactggt ttggctatct gattattgtc
    16261 atccaatggg gaacgaccat ctttggtatt ctctataaaa tctttgctaa aaaggtcaat
    16321 gagaaattta gccttgctct ttatctgatt atgggctggt tggttctggc tatcattcct
    16381 gccattatca gtcaaacgac acccgttttc tggagtctca tggtaactgg cggactctgt
    16441 tatacagttg gagctggatt ttatgccaag aaaaaacctt atttccacat gatttggcat
    16501 ctctttatcc tagctgcgtc cgcacttcaa tacatcgcta ttgtttatta catgtaaaaa
    16561 agttgagaaa ttcaatctca acttttttct ttacacatat tgataaagta ctggtgcaag
    16621 cgcacatcat cagtcaattc tggatgaaaa gaacttacca acatattttt ttcttgggct
    16681 gcaacaattt gattgttcac tgttgctaga atttctacac cctcaccaac actactgata
    16741 atcggaccac ggataaaggt cattggaatc ttgccaactc ccttacattc tgcttccgtg
    16801 tagaaacttc ctaattggcg cccataagca ttacgctcga ccaccatatc catagttcct
    16861 agatgactct ctttctgaga agtgatttcc ttagccagca aaattaagcc cgcacaggtc
    16921 ccaaacactg gtaagccaga tagaatggct tctcggatgg gaagtagcat gttctggtca
    16981 cgtaagagct tgcccatggt tgtagactca ccaccaggca aaatcaaacc cgacaagtca
    17041 ctctgatctt gctgaaaatc atctagattt ctgagttcta cactctcgac acctaattga
    17101 tctagcactt ttgcatgttc tgcaaaggcc ccttgcaagg ccaatattcc gattttcatc
    17161 tattttcctc gttcagccat gagaatttgg atttcatttt cattaatacc aaccatggct
    17221 tctcctaaat cttcagagat ttgagctagg atttgaggat tacggaagtt agtcacagcc
    17281 ttaacaatgg cactcgctcg tttaacagga tctcctgact tgaaaatacc tgaaccgaca
    17341 aagaccccct ctgcccctaa ttgcatcatt aacgcagcat ctgctggcgt tgcaacacct
    17401 ccagcagcga aatttacaac tggcaatttt ccatgttcat gaacatattg gaccaattct
    17461 acagggactt gcaaatcctt ggcagcaaca taaagctcgt cctcacgtaa gttttgaatg
    17521 cggcgaattt cctgattcat catacgcata tgacgaacag cttggactat atcccctgtc
    17581 cctggttctc ctttggtacg aatcatggaa gcaccttcag cgatacgacg caaggcttca
    17641 cccaaatcct tagcaccaca gacaaaagga acttggaatt ctttcttgtc cacatggaaa
    17701 cggtcatcag ctggagatag aacttcactc tcgtcgatat aatcaatttc aatagcctct
    17761 aaaatctgag cttcaacaaa atgcccgatt ctgaccttag ccattactgg aatactaacc
    17821 gcttcttgga tttccttaat catctttggg tcgctcatgc gggaaactcc tccagctgca
    17881 cgaatatcag ccggaattcg ttccaaggcc atcacagctg ccgcaccagc agcttctgcg
    17941 atacgagcct gttcaggatt ctgcacatcc ataataacac cacccttgag catctgtgcc
    18001 aagtttttat ttagttcata acgattttca gtcatttgtt ttatcctcat catttgtatt
    18061 ggtagctacc ttttcatttt aagtcagaac agaatgaagc acaagataaa aaaactataa
    18121 ttgtatgggg acagattagc taaaaactat ctgaccttaa cttaaggcca gatagctcat
    18181 tcatttttat ttttcagccg taagggcagc cattgtgatg tagttgtatg gtttgttgaa
    18241 gtgtggcaag aagaagaggt ctgtcaatgc caatttatca attgtcacat gctcttggat
    18301 agcaagtgag aacatgtgga ttcccatgct aattgcaata tcatgtgaaa ccatttgggc
    18361 accaagaatt tcacggctat ctttgtcaaa gacaatctta attgctactt catggttgtc
    18421 atgtttcatg aattctggtt tttgaagatc gttaaagcct gtttcagttg cgttgtaacc
    18481 agcagctttc gctttttcaa gagtcaaacc agttgaaacc atgtgaagac cgtagattga
    18541 gataccattt gaaccttgaa caccgattcc ttccaattca tgtccacaag cgttgtaggc
    18601 accaacgata ccagtgcgaa cagcatttga agcaagagcg atatagcttg tatctttacg
    18661 agcattgtca taaacagtcg cacagtcacc aacagcgtaa acgcctggga tagatgtttc
    18721 ttgtttcttg tctacaagga aggcaccgtt gcggaagagt tcgatcttac catctgcaag
    18781 ggctgtgttt ggacggaaac caactgcaag gataaccata tccacgtcaa agctttcttt
    18841 gtcagtaatc aagcgttcaa ctttaccgtc accttcgatt gctttaacag tttgacctag
    18901 agccaagcgg atgttgtgat cttccaagtt cttcgccatc atttgtgtga agtctttgtc
    18961 atagtaaccg ttcaagacag tatcaacgat atcaacaagg acaacttctt ttccaagacg
    19021 ttcaaaggct tcagcaagtt caacaccgat gtaaccacca ccaacaacgg cgatacggtc
    19081 gaggtgttgg ctcttgtcag aaagtttatt gataacttct tcagcatttt ggtacaattt
    19141 cacgaattgt acgtttttaa gagttgcttt aaattcgcgg tttcctttaa caatttcaac
    19201 accttcgatt ggtggcaaga ttggtgtaga acctgtagcg aaaatcaatt tttcgtatga
    19261 ttctttgtgc tcttttcctt caacttccgc tgtaactact ttgttatcat agtcgattga
    19321 aagaacaggt gagttcatgt aaactttagc acctttagct tccaattttt ctttatcaga
    19381 atagaacaag ccttcagcac cgtcaatttg ttcaccaatc caaagagcca ttccacatcc
    19441 taggaaagag atgttagagt tttggtcaaa tacaacaatt tcgttctcat ttccaaaatt
    19501 atccaacatg gtattgatac atgctgtacc agcgtggtta gcaccgacta caacgatttt
    19561 actcataaaa atttcctgcc ttcattctaa atttacctta ctagtatacc atttaccgtt
    19621 agcgtttaca aggggggtac tattatttca gcatttttta tcaaaaataa ttccccattt
    19681 atcctgtttt caagcctcat gaattttatg agcatatttc ttgacttttt ccaaattttg
    19741 tttgtgaaaa ttctaacttt atggtattat aataacatga aaatgaaatg taagggcttc
    19801 aatttagatg attatttgat attacaactt attttctata agagaaagga gttggtagct
    19861 tgactcttcg ttcacattct gaacggctaa tggggactac tatcactatt tcattagtgg
    19921 atgagcaagc cgatatcttt ctccaaaaat cctttgactt gctcaaagaa cttgaatacc
    19981 gcttcaacgc caatagtcaa gaatctgagt tgatggaaat caattatcaa gcaggaatag
    20041 cccccgtcac ggttcatcca gacctgtttg agctgatttc acttgggtta gagcatagcc
    20101 tagcgctctc tagccacctc aatatcagca ttggtccctt aattcaaacc tggcgtatcg
    20161 gtttttcaga tgccaaggtc gcccagcctc aagaaattga atcggtgctg cctctaatca
    20221 atcctcatgg tatcgagtta gattcttcta cttctactgt gtttttaaaa cagaaaggaa
    20281 tgaagatcga tctaggttgt ttagccaaag gatacagtgc ggataaggtt gcccaatttc
    20341 ttaggaaaga gggggtgact tctgccttga tcaatctggg agggaatatc ctgaccattg
    20401 gaaaaaatca ggcaagaggg gataacccat ggcaaatcgg gattcaagac ccagccaatc
    20461 ctaggggaaa tcatttaatg accatccctg ttgtcaataa atctgtcgtg acttcaggca
    20521 tttatgaacg tcacctgacc gtcgatggac aagattacca tcacattttt gacagtcaaa
    20581 caggatatcc tgttgaaacg gaactagcga gtctaacaat catctctgat aaatcagtcg
    20641 atggcgaaat ctggacgact cgactctttg gagaaagacc tgcttctatc ctctggcaag
    20701 tcgaaagttt ggagggcatc gaagtcatcc tcatcgataa agaaggccac ctaagctgtt
    20761 cttcaggaat tcctactcta tagagacaac tgtttcaatg gaattttaaa attgtattat
    20821 gtaaaaagag aaaggaaatc tcatgttaaa acttattgct attgttggaa caaattcaaa
    20881 acgttctaca aaccgtcaat tgcttcaata catgcaaaaa cactttgctg acaaagctga
    20941 aattgagctt gttgaaatca aggccattcc tgtcttcaac aaaccagctg acaagcaagt
    21001 acctgctgaa atattggaaa ttgctgctaa aatcgaagag acagatggcg ttattatcgg
    21061 tactcctgag tatgatcact ctattccagc tgttttgatg agcgctcttg cttggttgtc
    21121 ttatggtatt tacccacttt tgaacaaacc aatcatgatt acaggtgctt cttacggtac
    21181 gcttggttca tctcgtgccc aattgcaact tcgtcaaatc ttgaatgctc ctgaaatcaa
    21241 ggcaaatgtt cttccagatg aattcttgct ctcacactct cttcaagcat ttaacccaag
    21301 tggcgacttg gttgaccttg atgttatcaa gaaattggat gccatctttg atgacttccg
    21361 tatctttgta aaaatcacag aaaaattacg taatgcacaa gaattacttc gcaaagatgc
    21421 tgaagacttt gactgggaaa atttgtaaga taggagacca aaaagaatga aatttgttgg
    21481 acttgttgga tcaaactacg atcaatcata taaccgtaag ctcttggaat ttatccgtcg
    21541 ctacttcaaa ctcaaatttg aattagaagt tcttgaaatt gacgaagttc caatgtttaa
    21601 ccaagatgaa aaatgggatg aaagtttcca attacgttat ttatataaca aaattactcg
    21661 tgcagatggt gttatcattg ctactcctga gcataaccac actatctcag cttcactcaa
    21721 atctgtactt gaatggcttt catacgaagt tcatccattt gaaaacaagc ctgttatgat
    21781 tgtgggtgca tcatactatg accaaggaac atcacgtgct caagttcacc ttcgtaaaat
    21841 ccttgatgct ccaggtgtta atgcctacac gcttccaggt aacgaattcc ttcttggtaa
    21901 agctaaggaa gcttttgata acaacggaaa tatcaccaac gaaggcactg ttaaattcct
    21961 tgaaacttgc ttagataact ttgttaaata cgtaggagtc gtttcgaaat tgaaaaaacc
    22021 aaaaccaatc gaatcagaag acttggattg tggaaaacca atcgctacaa ccatcacaga
    22081 agttgaccct gacgatccag aatgggtaga aaaagttgca gcaatcactg gtgctgtttc
    22141 tggtgatacc tatgtcaaat tggaccacgg tatcctaaca gttaaccaaa ttgatatgtt
    22201 cttgaaagct atgccatttg aattgacata cgctgacgat aacaaccaat tcctctacta
    22261 caacaacgct caccaagacc cagacactat gttcgctaaa cgtgtaccac ctcaatcagg
    22321 tagccgtatg tcgactgttc atggttctct tccaccagca cgtatgaaga acgtagagtg
    22381 ggttatcgga acacttcgca acggaaacca agaatacgtc cgtacgatcg ttccaggttc
    22441 tcctgcaggt gttatcaaca ctcacaacta ccaagcaatg tactatcctg atggatcata
    22501 cgctggtatc aatgaaatcg tctttaactt ccaaccatgg cttgactggt acctaaaaga
    22561 gactggtcaa cgtttggtgg gtggtagcgg accatttgct cctgctgccg gaggtcatgg
    22621 agacgccgat gctacttctg gcgcttctga ttcaggggat gctggaggcc acggtggtga
    22681 cgcagatgct acagccggtg caagttacta atgatagttt ttaggaacca ttatggttcc
    22741 ttttttagcg aaaaaaga
//
LOCUS       DAVGJJ010000027        21942 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000027 DAVGJJ010000000
VERSION     DAVGJJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.027"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..505
                     /locus_tag="VVE35_000938"
     CDS             32..505
                     /locus_tag="VVE35_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU3061155.1"
                     /translation="MKKYTEVFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGN
                     TEKYIATYVNQEFKSYIDHMLNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNG
                     LGLFSQFLISVHYDSWNDKANKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            787..1035
                     /locus_tag="VVE35_000939"
     CDS             787..1035
                     /locus_tag="VVE35_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEU3061156.1"
                     /translation="MCKPISIELCDDEVHSLHEWIDGRDAIDSILTYSENQQYTYGVE
                     AGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            1211..1437
                     /locus_tag="VVE35_000940"
                     /pseudo
     CDS             1211..1437
                     /locus_tag="VVE35_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1593..2822
                     /locus_tag="VVE35_000941"
     CDS             1593..2822
                     /locus_tag="VVE35_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU3061157.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2842..5538
                     /gene="ppc"
                     /locus_tag="VVE35_000942"
     CDS             2842..5538
                     /gene="ppc"
                     /locus_tag="VVE35_000942"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU3061158.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLTEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5621..5838
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5951..6955
                     /locus_tag="VVE35_000943"
     CDS             5951..6955
                     /locus_tag="VVE35_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3061159.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLSVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIALTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7024..7890
                     /locus_tag="VVE35_000944"
     CDS             7024..7890
                     /locus_tag="VVE35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061160.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGLNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7887..8645
                     /locus_tag="VVE35_000945"
     CDS             7887..8645
                     /locus_tag="VVE35_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061161.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8735..10495
                     /gene="dnaG"
                     /locus_tag="VVE35_000946"
     CDS             8735..10495
                     /gene="dnaG"
                     /locus_tag="VVE35_000946"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU3061162.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10498..11607
                     /gene="rpoD"
                     /locus_tag="VVE35_000947"
     CDS             10498..11607
                     /gene="rpoD"
                     /locus_tag="VVE35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU3061163.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11621..11950
                     /locus_tag="VVE35_000948"
     CDS             11621..11950
                     /locus_tag="VVE35_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU3061164.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12065..13108
                     /gene="cpoA"
                     /locus_tag="VVE35_000949"
     CDS             12065..13108
                     /gene="cpoA"
                     /locus_tag="VVE35_000949"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU3061165.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13124..14449
                     /locus_tag="VVE35_000950"
     CDS             13124..14449
                     /locus_tag="VVE35_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU3061166.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDVNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14480..14656
                     /locus_tag="VVE35_000951"
     CDS             14480..14656
                     /locus_tag="VVE35_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU3061167.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14715..14849
                     /locus_tag="VVE35_000952"
     CDS             14715..14849
                     /locus_tag="VVE35_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU3061168.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14913..16223
                     /gene="obgE"
                     /locus_tag="VVE35_000953"
     CDS             14913..16223
                     /gene="obgE"
                     /locus_tag="VVE35_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU3061169.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGITKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16233..16391
                     /locus_tag="VVE35_000954"
     CDS             16233..16391
                     /locus_tag="VVE35_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061170.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(17410..17484)
                     /locus_tag="VVE35_000955"
                     /pseudo
     CDS             complement(17410..17484)
                     /locus_tag="VVE35_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            17627..18886
                     /locus_tag="VVE35_000956"
     CDS             17627..18886
                     /locus_tag="VVE35_000956"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU3061171.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFDEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18902..19453
                     /locus_tag="VVE35_000957"
     CDS             18902..19453
                     /locus_tag="VVE35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061172.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19446..20723
                     /locus_tag="VVE35_000958"
     CDS             19446..20723
                     /locus_tag="VVE35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3061173.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20739..21599
                     /locus_tag="VVE35_000959"
     CDS             20739..21599
                     /locus_tag="VVE35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU3061174.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6764 a         3717 c         4913 g         6548 t
ORIGIN      
        1 tacagttttt cgagaaaatt gggaaccgta catgaaaaag tacacggaag tttttcaaag
       61 tcaatttcct aatctaaaca ttgattactt gctcctggac actgagcaga ttgatcttga
      121 tagctatcta gatgctgaca taattatcat cggtggtgga aatacggaaa aatatattgc
      181 tacttatgtt aatcaggagt tcaaaagtta tatcgatcat atgcttaata aagaggcaaa
      241 aattatagga ttttctgcag gagccctact attaggagaa aaagtctatg tctcacctaa
      301 tgataattca gatcatcaga taaagataaa aaatggatta ggactcttta gtcagttttt
      361 aattagtgtc cattatgatt cctggaatga taaagcaaat aaggatagag ctgaagaact
      421 cgttaatgtt cccataattc cactaaatga tcattcttgt cttgtattgg ataaacttgg
      481 aaacatcata gagaaaattg actaactttc ataatgtaaa aatggatcta ggaaataaaa
      541 gtgtagacga actggatagg ttactagtga aaactgcaaa aagtgttgta aagtgaatcg
      601 gagtgtgctt gatggatttt agtagtaaga tagccataaa taaaggctgg tcagatgata
      661 aaaaatattg tgtgacagat caaaatcagt aaaaatattt cttgcgtgtt tctgataagt
      721 cgaagttaga ttataaaaaa atttgaattt gatatgatgg agaaagtagc ttctcttgga
      781 gttccgatgt gtaaaccaat tagcattgaa ctctgcgatg acgaagtaca ttctttacac
      841 gaatggatag acggaagaga tgcaatagat agcattttaa cttattcaga aaaccaacaa
      901 tacacatatg gtgtagaagc aggaaagata cttagaaaga ttcatacaat tcctgctaca
      961 gaagtttgtg aagattggga aatctttttt aatctaaaaa ttgatgataa aatctccaac
     1021 gaaatgatat ggtaagtccg aaacgctcag tgtgtaagct tgttgctaaa agcgtaaaaa
     1081 ccttggaacg aaaggaataa tagacttcct gcgaaacaaa aatacaatac aataaaacta
     1141 tggatgatga agcaagtaaa caattaagcg atagccgttt caagagcctt gtaggtgttc
     1201 agcgcacgag atgttagctg tgttaaaaac agcttatcaa cgcaacgtaa acgcgcaaaa
     1261 ggtggacgaa aaagcaaatt aagcctagac gatctcctta tggtaactat tcaatacatg
     1321 cgagaatagc gcacttatga acaaattgcg gctgattttg gcattcacga aagcaacata
     1381 attagtcgga gtcaatggat tgaagcaact cttattcaaa atggttttac gatttcaaat
     1441 tctgccttaa ttctgtaaaa acagtaaaat tcgaaggatt gtaaggtaag agtttttttc
     1501 tttctgaaaa aatggtataa tagcaatcaa aactagaaaa taaaacggaa tttggaacag
     1561 atttgtctgt atcctagtag agtggtgata ctatgaagat tagtaagagg cacttattaa
     1621 attattccat cttgattccc tacttgcttt tatctatttt gggcttgatt gtggtctatt
     1681 cgaccaccag tgctatttta attgaagaag gcaagagcgc cttgcagttg gttcgaaacc
     1741 aaggaatctt ttggattgtt agtttgatac tgattgcctt aatttataaa ttgagactag
     1801 attttttgag aaatgagcga ctaatcattt tagttatatt aatagaaatg cttttattgt
     1861 tcttggctcg ttttattggt atttcagtaa acggggcata cggttggatt tcggttgcag
     1921 gagtaactat tcagccagct gagtacttaa aaatcattat tatttggtat ttagctcacc
     1981 gattctccaa acagcaagaa gaaatagcta cttatgattt tcaagttttg actcaaaatc
     2041 aatggcttcc ccgtgctttt aatgattggc gattcgttct cctagttctg attggaagtt
     2101 tgggaatttt ccctgattta ggaaatgcga ctattttagt cttggtttcc ttgattatgt
     2161 atacagttag tggaatcgct tatcgctggt tttcaaccat tctggcgctc gtatctgcca
     2221 cttctgtctt tgtcttgacc actatcagcc taatcggtgt tgagaccttt tcaaaaattc
     2281 cagtatttgg ctatgtagcc aagcgcttta gtgccttttt taatcctttt gccgatcgtg
     2341 ctgatgcagg tcaccagtta gctaattctt attttgccat ggtcaatggt ggttggtttg
     2401 gtctaggtct tggaaactcg attgaaaaac gaggttattt gccagaagct catacagact
     2461 ttgtcttttc tatcgtgatt gaagaatttg gctttgttgg tgccagtctt attttagctc
     2521 tcttgttttt catgattttg cggattatct tggtcggtat ccgagcggag aatcctttca
     2581 atgccatggt tgcactcggt gtcggaggga tgatgttggt tcaggtattt gtcaatatcg
     2641 gagggatttc gggcttgatt ccatctacag gagtgacttt ccctttctta tcccagggtg
     2701 gaaatagtct tctagtctta tcagtggcag tagcctttgt cttaaatatt gatgccagtg
     2761 aaaaacgcgc taagttgtac cgagaattgg aaaatcaacc aatgaacctt ctgttgaagt
     2821 aggataaaga aaggatagtt tatgtctctt caaaaattag aaaattatag taataaaagt
     2881 gttgtgcaag aagaagtctt gattctaaca gaattactgg aagatattac taaaaatatg
     2941 cttgccccag agacctttga aaaaataata cagttgaaag aattatcaac gcaggaagat
     3001 tatcaaggtc taaaccgtct agtgactagc ttatcaaatg atgaaatggt ctatatttca
     3061 cgctatttct ctatcttacc tcttttgatt aatatttcag aggatgtgga tttagcttat
     3121 gaaatcaatc atcaaaataa tattgatcag gactatttag gtaaattatc tacaacgatt
     3181 aaattggtag cagaaaagga aaatgccgtt gagatcctag aacacttgaa tgttgtccct
     3241 gttttgacag cccatccaac acaagtgcaa cgcaaaagta tgttggattt aacaaatcat
     3301 attcatagtc ttttgcgtaa ataccgtgat gttaagttgg ggttgatcaa taaagataaa
     3361 tggtacaatg atttgcgtcg ttacatcgaa attatcatgc agacagacat gattcgtgag
     3421 aaaaaattaa aagtgactaa cgaaatcacg aatgctatgg aatattataa cagctccttt
     3481 ttgaaagctg tacctcattt gacgacggag tataagcgct tagcgcaagc gcatggtctg
     3541 aatttaaaac aggctaaacc aatcaccatg ggtatgtgga taggtggtga ccgtgatgga
     3601 aatccatttg ttacagcaga gaccttgaag cagtctgcac tcactcaatg tgaagtcatc
     3661 atgaactatt atgataaaaa gatttaccaa ctttatcgtg aattttctct ttcaactagc
     3721 attgtcaacg tcagcaagca agtcagagaa atggctcgtc aatccaagga taactcgatt
     3781 taccgcgaaa aagagcttta ccgtcgtgcc ttgtttgata ttcaatcaaa aattcaggca
     3841 actaaaacct atctgactga ggatgaagaa gttgggactc gttatgaaac cgccaatgat
     3901 ttctacaagg atttgattgc cattcgagat tctctactag aaaataaggg cgagtccttg
     3961 atttcaggtg attttgtgga attattgcag gcagtagaga tatttggttt ttacttagca
     4021 tcaattgata tgcgacaaga ctctagcgtc tatgaagcct gtgtggcaga actcttgaaa
     4081 tcagcaggaa ttcattctcg ttatagcgag ttgagcgaag aagaaaagtg tgaccttctc
     4141 ttgaaagaat tagaagaaga tccccgaatt ctttctgcga ctcacgcaga aaaatcagaa
     4201 ttattagcaa aagaattagc tatttttaag acggctcgtg ttttgaaaga taagttggga
     4261 gatgatgtca tccgtcagac catcatttca catgcaacca gcctttctga tatgctagaa
     4321 ttagctattc tgttaaaaga agtaggactg gtggatacgg aaagggcgcg tgttcagatt
     4381 gttccccttt ttgaaacaat tgaagacttg gatcattcag aggaaacaat gagaaaatat
     4441 ctttctctta gccttgccaa aaaatggatt gactcacgaa ataactacca agaaatcatg
     4501 cttggctact ctgacagtaa taaagatggc ggttacttgt catcatgttg gaccctctac
     4561 aaggctcaac aacaattgac tgctattgga gatgaatttg gcgttaaggt taccttcttc
     4621 catggtcgtg gtggtactgt cggtcgtggt ggtgggccaa cctatgaagc tattacatct
     4681 caaccgctca agtctatcaa ggatcgtatc cgcttgacgg agcagggtga agtaattggg
     4741 aataaatacg gtaataaaga cgccgcttac tataaccttg aaatgctagt atcggcagct
     4801 attaaccgta tgattactca gaagaagagc gataccaata ccccaaatcg ttatgaagcc
     4861 attatggatc aagtagtgga ccgtagttac gatatctacc gtgatttggt ctttggtaat
     4921 gagcatttct atgattattt cttcgagtca agtccaatca aggctatttc aagttttaat
     4981 attggttctc gtccagccgc tcgtaagact attactgaaa tcggtggttt gcgtgccatc
     5041 ccttgggtat tctcatggtc acagagtcgt gttatgttcc ctggatggta cggggttggt
     5101 tcaagcttca aggaatttat caataaaaat ccagagaata ttgctatctt acgagatatg
     5161 taccaaaatt ggcctttctt ccaatcgctt ctttcaaatg ttgatatggt tttgtcaaaa
     5221 tcaaatatga atattgcttt tgaatatgct aaactttgtg aagacgagca agttaaggcc
     5281 atctatgaga ctattttaaa tgaatggcaa gttactaaga acgttatctt ggctattgaa
     5341 ggacatgacg aactcttagc tgacaatcca tatctaaaag ctagtctgga ttaccgtatg
     5401 ccttacttta atattctcaa ctatattcag ttagagttga ttaaacgcca acgtcgtgga
     5461 gaattgtcca gtgatcaaga acgattgatt catatcacca tcaacggaat tgcgacagga
     5521 ttgcgtaatt caggttgata atttccaaga gtgaatgcta aaagtgaata tcaaaaaaat
     5581 tctaatagac tattgacaag tagtttaaaa atgatataat ttaaccattc agaaaagtaa
     5641 tcatacaaac tttttagaga gtctgtggta gctgaaaaca gataagtggc gatgatgaaa
     5701 attgggctga atgctattta gaatttgaaa ttataaaaat tcggtaagca caccttacag
     5761 tgcatctcgt tattgcgaga ctgagcgata gggaaattcc ctataattgg ggtggtaccg
     5821 cgcatcgacg tcctcacaca agttttttgt gtgaggattt ttttgatgga ggttagtatg
     5881 gaaagaaaac gatggcgtcg cttgtttaga taagtgaaat atgttaaagg aaataaaaag
     5941 gagaaacaga atgaaaaata aacgtttaat tggaattatt gctgcattag cagtcttagt
     6001 agcaggaagc ttgatttatt cttcaatgaa taaatcagaa gctcagaata ataaggatga
     6061 gaagaaaata accaagattg gtgtgcttca atttgtgagc catccatccc ttgatttgat
     6121 ttataaaggg atccaagatg gacttgcaga agaaggatat aaagatgatc aagttaaaat
     6181 tgattttatg aactcagaag gtgaccaaag taaggttgcg acaatgagta aacaattggt
     6241 tgcaaatggg aatgaccttg tggttggtat cgcaacacca gcagcccaag ggttggctag
     6301 tgcaacaaaa gacctatcgg ttatcatggc cgctattaca gacccaattg gtgctaactt
     6361 ggttaaagat ttgaaaaaac caggtggcaa cgttacagga gtatctgacc acaatccagc
     6421 tcaacaacaa gttgaactca tcaaggctct gacaccgaat gtgaaaacaa tcggagctct
     6481 ttactcaagt agcgaagaca attcaaaaac acaggtcgaa gaatttaagg cttatgctga
     6541 aaaagcaggt ctgacagtgg aaacatttgc agttccttca acaaatgaaa ttgccttaac
     6601 tgtcactgtt atgactagca aggtagatgc tatttgggtt ccaattgata acaccattgc
     6661 atcaggattt ccaacggttg tctctagcaa tcaaagttct aagaaaccaa tttatcccag
     6721 tgcgacagct atggtagaag taggtggttt ggcatcagtt gtaattgacc aacatgacct
     6781 tggtgtggca acaggtaaaa tgattgtgca agtcttgaaa ggtgcaaaac cagccgatac
     6841 cccagtcaat gtcttttcaa ctggtaagtc agtcatcaat aaaaaaatag cacaagaact
     6901 aggtattact attcctgagt ctgttctcaa agaagcagga caagtcatcg aataaataag
     6961 gtagggagga ggagactctt cccatttttt aaaaagatga atatagaaag gattaaataa
     7021 aatatgattg tttccattat ttctcaagga tttgtctggg ctattctagg tctgggaatc
     7081 tttatgacat ttaggatttt aaactttcca gatatgacga cagaaggttc cttccctctt
     7141 gggggagctg ttgctgtcac tttgataacc aaaggcgtga acccattttt agcgacactt
     7201 gttgctgtag gagcaggttg tttggctgga atggcagcag gccttcttta tacaaaaggg
     7261 aagatcccaa ccttgctctc agggattttg gtgatgactt cttgtcactc aatcatgctc
     7321 ttgattatgg gacgtgcgaa tttaggcctg cttggaacca agcaaattca ggatgttttg
     7381 ccttttgatt cggatttgaa tcaactcttg acaggtctca tctttgtgag tattgttatt
     7441 gctctcatgc tctttttctt ggacactaaa ctcggacaag cctatattgc tacaggggat
     7501 aatcctgata tggctagaag tttcgggatt catactggac gcatggagct catgggcttg
     7561 gtcttatcaa atggtgtgat tgcccttgca ggtgccctca ttgctcagca agaaggttat
     7621 gccgatgtgt ctcgagggat cggggttatc gttgtggggc ttgcaagttt gattattgga
     7681 gaagttattt tcaagagttt gagcttggca gagcgtttgg ttactatcgt tgtaggttct
     7741 atcgcttatc aatttttagt gtgggcagtt atcgcacttg gccttaatac aagttacctt
     7801 cgtttataca gtgccttgat tttagcagtc tgcctcatga ttccaacatt taagcaaaca
     7861 atcttgaaag gagccaagtt aagcaaatga cagcaattgt agaattaaaa aatgcaacca
     7921 aaatcgttaa aaatggcttt gatgaagaaa agattatttt aaatgatgtt tccttagaaa
     7981 tttttgaaca ggactttatc acgattttgg gtggaaatgg tgctggtaaa tcaaccctct
     8041 ttaacactat tgcaggaacc atatcactaa ccagtggaac tatccgtatt ttaggtgagg
     8101 atgttaccaa gttttcaccc gagaagcgtg ccaagtacct gtctcgtgtc ttccaagatc
     8161 caaagatggg gacagctccc cgtatgacgg tcgctgaaaa tcttttaatc gccaagtttc
     8221 gtggtgaaaa gcgtggattg ttaccacgac gcttgactag ctataaggat gaatttcagg
     8281 caaccattga aaaagtagga aatggtcttg agaaacactt gaatacaccg attgagttct
     8341 tatcaggtgg acaaagacag gctttgagtc tcttgatggc aaccttgaag cgacctgaat
     8401 tactcctgtt agatgagcat actgctgccc tggatccaaa gactagtgtt gctttgatgg
     8461 aattgacaga tgaatttgtt aagaaagatc agctaacagc ccttatgatt actcatcata
     8521 tggaagatgc tctcaaatac ggcaatcgct tgattgtcat gaaagaagga cggattatcc
     8581 aagatttaaa ccaagaagaa aaagcaaaaa tgaaaatctc tgattattat caactctttg
     8641 aataaagtag ggaaaatgag tgaaatggtt tacttttttt ctgaaataaa gtatactata
     8701 taaagtaaac tatgataaca tggaggtatt gtgtatggtt gacaaacaag tcattgaaga
     8761 aatcaaaaac aatgccaaca ttgtggaagt cataggagat gtgatttctt tacaaaaggc
     8821 aggacggaac tatctagggc tctgtccttt tcatggtgaa aaaacacctt ctttcaacgt
     8881 tgtagaggac aagcagtttt accactgttt tggttgtggt cgctcaggtg atgtctttaa
     8941 attcatcgag gagtaccaag gggttacctt tatggaggct gtccaaatct taggtcagcg
     9001 tgtcgggatt gaggttgaaa aaccgcttta tagtgaacag aagccagcct cgcctcacca
     9061 agctctttat gatatgcacg aagatgcggc taaattttac catgctattc tcatgacaac
     9121 gactatgggc gaagaggcca gaaattacct ttatcagcgg ggtttgacag atgaagtgct
     9181 taaacatttt tggattggtt tagcacctcc agaacgaaac tatctctatc aacgtttgtc
     9241 tgatcagtat cgtgaagaag atttactgga ttcaggcctg ttttatctct cggatgccaa
     9301 tcaatttgta gacacctttc acaatcgcat tatgtttccc ctgacaaatg accaaggaaa
     9361 ggtcattgcc ttctcaggtc gtatctggca aaaaacagat tcacaaactt ctaagtataa
     9421 aaacagccga tcgactgcaa tttttaacaa aagttacgaa ttatatcata tggatagggc
     9481 aaaaaaatct tctggaaaaa ctagtgagat ttacctgatg gaaggattca tggatgttat
     9541 tgcagcctat cgggctggaa tcgaaaatgc tgtggcgtcg atgggaacgg ccttgagtcg
     9601 agagcatgtt gagcatctga aaaggttaac caagaaattg gttcttgttt acgatggaga
     9661 taaggctggg caagccgcga tattgaaagc attggatgaa attggtgata tgcctgtgca
     9721 aatcgttagc atgcctgata acttggatcc tgatgagtat ctacaaaaaa atggtccaga
     9781 agacttggcc tatctattaa cgaaaactcg tattagtccg attgagttct acattcatca
     9841 gtacaaacct gaaaacagtg aaaatctgca ggctcagatt gagtttattg aaaaaatagc
     9901 tcccttgatt gttcaagaaa agtccatcgc tgctcaaaac agctatattc atattttagc
     9961 tgacagtctg gcgtcctttg attatgccca gattgagcag attgttaatg agagtcgtca
    10021 ggtgcaaagg cagaatcgca tggaaggaat ttccagaccg acgccaatca ccatgcctat
    10081 caccaagcag ttatcggcta ttatgagggc agaagcccat ctactctatc ggatgatgga
    10141 atcccctctt gttttgaacg attaccgttt gcgagaagac tttgcatttg ctacacctga
    10201 atttcaggtc ttatatgact tgcttggcca gtatggaaat cttcctccag aagttttagc
    10261 agagcagaca gaggaagttg aaagagcttg gtaccaagtt ttagctcagg atttgcctgc
    10321 tgagatatcg ccgcaggaac ttagtgaagt agagatgact cgaaacaagg ctctcttgaa
    10381 tcaggacaat atgagaatca aaaagaaggt gcaggaagct agccatgtag gagatacaga
    10441 tacagcccta gaagaattgg aacgtttaat ttcccaaaag agaagaatgg agtaataatg
    10501 gcaacaaaac aaaaagaagt aacaacattt gacgtacagg tagcagaatt tatccgtaat
    10561 cataagcaaa aagggacagc aacagatgat gaaatcaatg caagtctggt tattcctttt
    10621 accttggacg ctgatgggat tgaagatctc ttgcaacgga ttcaggatgc aggcatttct
    10681 atcacagata acgaaggaaa tccaagtgcg cgtgttctta gcaatgaaga agaaccagaa
    10741 ctcagcgatg aggacttgat tgggtcaact tctgctaagg tcaatgaccc tgtccgtatg
    10801 tacttgaaag aaataggggt cgttcctctc ttgaccaatg aagaggagaa agagttggca
    10861 ctggctgttg aagctggcga tatcgaagcc aaacaacgtc ttgcggaagc caatcttcgt
    10921 ttggttgttt ccattgccaa acgctatgtc ggtcgtggca tgcagttcct tgacttgatt
    10981 caagaaggaa atatgggctt gatgaaggcg gttgacaagt ttgactattc taaagggttc
    11041 aagttttcaa cttatgcaac ttggtggatt cgtcaggcta tcactcgtgc tattgcggat
    11101 caagctcgta ccatccgtat cccagttcac atggttgaaa ctatcaataa attggttcgt
    11161 gaacagcgga atctccttca agaattgggg caagatccga caccagaaca gattgctgaa
    11221 cgaatggata tgacacctga taaggttcgt gaaatcttga agattgccca agaaccagta
    11281 tctcttgaaa ctcctatcgg tgaagaggac gatagccacc ttggagactt tatcgaagat
    11341 gaagtgattg aaaatccagt ggattatacg actcgtatcg tcttgcgtga gcaattggat
    11401 gaaatcttag atactcttac agaccgtgaa gaaaatgttc tgcgtctacg ttttggacta
    11461 gatgatggaa aaatgcgcac acttgaagat gtggggaaag tctttaacgt aactcgtgag
    11521 cgtatccgtc agattgaagc aaaggctttg agaaaactac gccaaccaag ccgcagcaaa
    11581 ccgcttcgtg attttattga agactaagag tgaggaaaat atggcttata cagaagagca
    11641 aattgaaaac atcaaaacac ggattttaac agccttggaa gaagtcatcg accctgagtt
    11701 gggaatcgat attgtcaatc ttggtttgat ctatgagatt cgttttgacg gtgacacagg
    11761 gcaaacagag attgacatga ctttgacaac tatgggttgt cccttagcag accttttgac
    11821 agaccagatt tatgatgcta tgatagaggt tccagaagta acggatactg aggttaaatt
    11881 agtctggtat ccagcctgga cagttgaaaa aatgagtcgc tatgcccgca ttgccctagg
    11941 cattaagtaa acatataaaa gcatgtgaga gactgttgga aaagcgagga aatttcccct
    12001 cttttcctct agtctctcct ttcttttgct gattttattc aaagaaaatg atataatagt
    12061 agttatggag aaaaagaaat tacgcatcaa tatgttgagt tcaagtgaga aagtagcagg
    12121 acagggagtt tcaggtgctt accgtgaatt agttcgtctt cttcaccgtg ctgccaagga
    12181 ccaattgatt gttacagaaa atcttccaat cgaggcagat gtgactcact ttcatacgat
    12241 tgattttccc tattatttat caaccttcca aaagaaacgc tcagggagaa agattggcta
    12301 tgtgcatttc ttgccagcta cacttgaagg aagtttgaaa attccatttt tcttaaaggg
    12361 aattgtgaaa cgctatgtat tttcttttta caaccggatg gagcacttgg ttgtggtcaa
    12421 tcctatgttt attgaggatt tggtagcagc tggtattcca cgtgaaaaag tgacctatat
    12481 tcctaacttt gtcaacaagg aaaaatggca tcctctacca caagaagagg tagtcagact
    12541 gcgcacagat cttggtctta gtgacaatca gtttatcgta gtaggtgctg ggcaagttca
    12601 gaaacgtaaa gggattgatg actttatccg tctggctgag gaattgcctc agattacctt
    12661 tatctgggct ggtggcttct cttttggtgg tatgacagat ggttatgaac actataagaa
    12721 aattatggaa aatcccccta aaaatttgat ttttccaggc attgtatcgc cagagcggat
    12781 gcgcgaattg tatgctctag cggatctttt cttgttgcct agttacaatg agctctttcc
    12841 tatgactatt ttagaagctg cgagttgtga ggctcctatt atgttgcgtg atttagatct
    12901 ctataaggtg attttggagg gaaattatcg ggcgacagcg ggtagagaag agatgaaaga
    12961 ggctattttg gaatatcaag caaatcctgc tgtcttaaaa gatctcaaag aaaaggctaa
    13021 gaatatttcc agagagtatt ctgaagagca tctgttacaa atctggttgg acttttatga
    13081 gaaacaagcc gctttaggga gaaagtaaaa agtgaggtaa tctatgcgaa ttggtttatt
    13141 tacagatacc tattttcctc aggtttctgg tgttgcgacc agtattcgaa ccttgaaaac
    13201 agaacttgaa aagcagggac atgctgtttt tatctttacg acgacagata aggatgtcaa
    13261 tcgctacgaa gattggcaaa ttatccgcat tccaagtgtt cctttctttg cttttaagga
    13321 tcgtcgcttt gcctaccgag gttttagcaa ggcacttgaa attgctaaac agtatcagct
    13381 agatattatc catactcaga cagaattttc tcttggcctg ttggggattt ggattgcgcg
    13441 tgaattgaaa attccagtca tccataccta tcacacccag tatgaagact atgtccatta
    13501 tattgctaag gggatgttga tccggccgag tatggtcaag tatctggtta gaggtttcct
    13561 gcatgatgtg gatggggtta tttgtcctag tgagattgtc cgtgacttgc tatctgatta
    13621 taaggtcaag gttgaaaaac gggtcattcc tactgggatt gaattagcca agtttgagcg
    13681 tccggaaatc aagcaggaaa atttgaaaga actgcgtagt aaactaggga ttcaagatgg
    13741 tgaaaagacg ttgcttagtc tttcgagaat ctcctatgaa aaaaatattc aagcagtttt
    13801 agcagccttt gctgatgttc tgaaagagga agacaaggtt aaactggtag tagctgggga
    13861 tggcccttat ctgaatgacc tcaaagagca agctcagaac ctagagattc aagactcagt
    13921 catctttaca gggatgattg ctcctagtga gacggctctt tactataaag cggcggattt
    13981 cttcatttcg gcatcgacaa gcgaaacgca aggtttgacc tacttggaaa gcttagccag
    14041 tggaacacct gtcattgctc acggaaatcc ttatttgaac aacctcatca gtgataaaat
    14101 gtttggaacc ttgtactatg gagaacatga tttggctggt gctattttgg aagccctgat
    14161 tgcaacacca gacgtgaacg agcatacctt atcagagaaa ttgtatgaga tttcagctga
    14221 gaactttggg aaacgagtgc atgagtttta tctggatgcc attatttcaa ataacttcca
    14281 gaaagatttg gctaaagatg atacggtcag tcagcgtatc tttaagacag ttttgtatct
    14341 tcagcaacag gtggttgctg tacctgtaaa aggatctaga cgcatgttga aggcttcaaa
    14401 aacacagttg atcagtatga gagactattg gaaagaccat gaagaataga aagaggaaca
    14461 gctatgaaaa aaacaattaa tgagaagcgg tcgtgataaa aagattgcgg gtgtttgtgc
    14521 tggggtggcc cattatctgg atatggatcc gactatcgtt caagtcattt ggggtgttct
    14581 tacttgctgt tacggagctg gaattgtagc ttacattatt ttatggatta tcgcaccagt
    14641 agcaactgac tattgaaaac tagctcaatt catgctaaga taatagaaaa tagaatgtaa
    14701 cgaaggagag aaaaatggca tttggagata atggaaatcg taaaaaaact atgtttgaga
    14761 aaataacctt gtttatcgtg attatcatgc tagtagcaag tttattggga atttttgcaa
    14821 ctgcaattgg tgccctcagt aatctataaa atagattcaa gaaaatttag tgactgggat
    14881 ttcccagccc ttttttaaag tgagaagaaa taatgagtat gtttttagat acagctaaga
    14941 ttaaggtcaa ggctggtaat ggtggcgatg gtatggttgc ctttcgtcgt gaaaaatatg
    15001 tccctaatgg aggcccttgg ggtggtgatg gtggtcgtgg aggcaatgtg gtcttcgttg
    15061 tagacgaagg actacgtacc ttgatggatt tccgctacaa tcgtcatttc aaggctgatt
    15121 ctggtgaaaa agggataacc aaagggatgc atggtcgtgg tgctgaggac cttagagttc
    15181 gagtaccaca aggtacgact gttcgtgatg cggagactgg caaggtttta acagatttga
    15241 ttgaacatgg gcaagaattt atcgttgccc acggtggtcg tggtggacgt ggaaatattc
    15301 gtttcgcgac accaaaaaat cctgcaccgg aaatctctga aaatggagaa ccaggtcagg
    15361 aacgtgagtt acaattggaa ctaaaaatct tggcagatgt cggtttagta ggattcccat
    15421 ctgtagggaa gtcaacactt ttaagtgtta ttacctcagc taagcctaaa attggtgcct
    15481 accactttac cactattgta ccaaatttag gtatggttcg cacccaatca ggtgaatcct
    15541 ttgcagtagc cgacttgcca ggtttgattg aaggggctag tcaaggtgtt ggtttgggaa
    15601 ctcagttcct ccgtcacatc gagcgtacac gtgttatcct tcacatcatt gatatgtcag
    15661 ctagcgaagg ccgtgatcca tatgaggatt acttagctat caataaagag ctggagtctt
    15721 acaatcttcg cctcatggag cgtccacaga ttattgtagc taataagatg gacatgcctg
    15781 agagtcagga aaatcttgaa gaatttaaga aaaaattggc tgaaaattat gatgaatttg
    15841 aagagttacc agctatcttc ccaatttctg gattgaccaa gcaaggtctg gcaacacttt
    15901 tagatgctac agctgaattg ttagacaaga caccagaatt tttgctctac gacgagtctg
    15961 atatggaaga agaagcttac tatggatttg acgaagaaga aaaagccttt gaaattagtc
    16021 gtgatgacga tgcgacatgg gtactttctg gtgaaaaact catgaaactc tttaatatga
    16081 ccaactttga tcgtgatgaa tctgtcatga aatttgcccg tcagcttcgt ggtatggggg
    16141 ttgatgaagc ccttcgtgcg cgtggagcta aagatgggga tttggtccgc attggtaaat
    16201 ttgagtttga atttgtagac taggagactg gtatgggaga taaaccgata tctttccgag
    16261 atgcggatgg taattttgtt tccgccgcag acgtttggaa tgaaaagaaa ttggaagaac
    16321 tatttaatcg tctcaatcca aatcgtgcct tgagattggc acgaactaaa aaggaaaatc
    16381 catctcagta aagaagctaa aaaatcccgt gcctcatcag acacgggatt ttgtggtacg
    16441 acgggcatgt atagcgaact gaatctggaa tagcacagca tatcttctaa aatatagtaa
    16501 aatgaaatga gaacaggaca aatcgatcag gacagtaaaa tcgatttcta acaatgtttt
    16561 agaagcagag atgtactatt ctagtttcaa tcaactatat tgttataaat tgatttgaat
    16621 ttcaaaatta aattgtttga ttcttatttc aatttgttat agtatatctg atgtcaaagt
    16681 tctcggcgag tcaaatagtg attcccaagc ctgactatcg tgaggtagcg gattaaaatg
    16741 gtctggggat agaccgtttt aagtctgacg ctggaaataa gaatcgtcag aagaagggat
    16801 agcgaaatcg tggctctacg aacaggaacg tgataataag gcgtatatag cggataagag
    16861 ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgcgtaaa tcacgagagt
    16921 aattgaattc gtactaagat tttctatttt cactataacc ttttaacgtc ctcatatctt
    16981 gtatacacga ggaaagatgt acgacttatc ccgtgaggtc tatcactata aagagaaaac
    17041 gacagataga agtcatcctg agtcacggtt atctttaaaa tagattgtct gataggatgc
    17101 tagcctgaaa acgtcttgtt catgaaatat aggacggtat gtataaaacg cttctgcgaa
    17161 ctgagagaag ggggagaagt tcttgctaaa atttagttga acagccgtat tccgatattt
    17221 agataaggga tctagtctta gctcctactc ggtttcaggg gataaaaaag gggcaatagt
    17281 gattcgagaa agattatact ctttgaaaat ctcttcaaat cacgtcaata ttgccttgcc
    17341 gtaggtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaccat gttttgagct
    17401 gacttcgtca gttctatcca caatctcaaa acactgtttt gagcaacctg cggctagttt
    17461 cctagtttga tctttgattt ttattgagta ttagtaattc agttactaac tcgtcaactc
    17521 tgatttatcc aataaaattg aaaaagatgg aaaaaaggat aaatttatga tatactttat
    17581 tttgaagacc ttattagaaa tcttgaaaga gtattgaaaa cttagaatga gaaaaattgt
    17641 tatcaatggt ggattaccac tgcaaggtga aattactatt agtggtgcta aaaatagtgt
    17701 tgtggcctta attccagcta ttatattggc tgatgatgtg gtgactttgg attgtgttcc
    17761 agatatttcg gatgtagcca gccttgtcga aatcatggaa ttgatgggag ctactgttaa
    17821 gcgttatgac gatgtcttgg agattgatcc aagaggtgtt caaaatattc caatgcctta
    17881 tggtaaaatt aacagtcttc gtgcatctta ctatttttat gggagcctct taggccgttt
    17941 tgatgaagcg acagttggtc taccgggagg atgtgatctt ggtcctcgtc cgattgactt
    18001 acaccttaag gcgtttgaag ctatgggtgc cactgctagc tacgagggag ataacatgaa
    18061 gttatctgct aaagatacag gacttcatgg tgcaagtatt tacatggata cggttagtgt
    18121 gggagcaacg attaatacga tgattgctgc ggttaaagca aatggtcgta ctattattga
    18181 aaatgcagcc cgtgaacctg agattattga tgtagctact ctcttgaata atatgggtgc
    18241 ccatatccgt ggggcaggaa ctaatatcat cattattgat ggtgttgaaa gattacatgg
    18301 gacacgtcat caggtgattc cagaccgcat tgaagctgga acatatatat ctttagctgc
    18361 tgcagttggt aaaggaattc gtataaataa tgttctttac gaacacctgg aagggtttat
    18421 tgctaagttg gaagaaatgg gagtgagaat gactgtatct gaagacagca tttttgtcga
    18481 ggaacagtct aatttgaaag caatcaatat taagacagct ccttacccag gctttgcaac
    18541 tgatttgcaa caaccgctta cccctctttt actaagagcg aatgggcgtg gtacaattgt
    18601 cgatacgatt tacgaaaaac gtgtaaatca tgtttttgaa ctagcaaaga tggatgcgga
    18661 tatttcgaca acaaatggtc atattttgta cacgggtgga cgtgatttac gtggggccag
    18721 tgttaaagcg actgacttaa gagctggggc tgcactagtc attgctgggc ttatggctga
    18781 aggtaaaact gaaattacca atatcgagtt tatcttacgt ggttattctg atattatcga
    18841 aaaattacgt aatttaggag cggatattag acttgttgag gattaaaccg tagaggtgtt
    18901 tatgaatatt tggaccaaat tagcaatgtt ttcttttttt gaaacggatc gcttgtattt
    18961 gcgtcctttc ttttttagtg atagtcagga cttccgcgag atagcttcaa atccagaaaa
    19021 tcttcaattt attttcccaa cgcaggcaag tctggaagaa agtcaatatg cactggccaa
    19081 ttactttatg aagtcccctt tgggagtgtg ggcaatttgt gaccagaaaa atcaacaaat
    19141 gattggttct attaaatttg agaagttaga tgaaatcaaa aaagaagctg agcttggcta
    19201 ttttttgaga aaagatgctt ggtcgcaagg atttatgaca gaggttgtta gaaaaatttg
    19261 tcagctttct tttgaggaat ttggcttaaa acaattattt atcattaccc accttgaaaa
    19321 taaagctagc caaagagttg ctcttaagtc tggatttagt ttgttccgtc agtttaaggg
    19381 aagtgatcgt tacacaagaa aaatgcggga ttatcttgaa tttcggtatg taaaaggaga
    19441 gttcaatgag taagcatcag gaaattctaa gctatttgga ggaattacca gtaggtaaaa
    19501 gggtcagtgt tcgtagcatt tcgaatcatc taggagttag tgatggaaca gcctatcggg
    19561 ctattaaaga agctgaaaac cgtggaattg tggagacccg tcctagaagt ggaacaattc
    19621 gtgttaaatc ccagaaagtt gctatagaga gattaacgtt tgctgaaatt gcagaagtga
    19681 cttcttctga ggttctggct gggcaagaag gtttagagag agaatttagt aagttttcaa
    19741 ttggtgccat gactgaacaa aatatcttgt cttaccttca tgatgggggg ctcttgattg
    19801 tcggagaccg aacccgtatt cagttgctag ccttggaaaa tgaaaatgca gttctggtta
    19861 cagggggatt tcaggttcat gatgatgtgc ttaaactggc caatcaaaaa gggattcctg
    19921 ttctaagaag taagcatgat acctttaccg tcgcgaccat gatcaataaa gccttgtcaa
    19981 atgtccaaat caagactgat attctgacag ttgagaaact ttatcgccct agtcatgagt
    20041 atggttttct gagagagaca gatacagtta aagattattt ggacttggtt cgtaagaatc
    20101 gtagcagccg tttccctgtt atcaatcaac atcaggtcgt tgttggtgtt gtaaccatga
    20161 gagacgctgg tgataaatca ccaagcacga caattgataa ggttatgtct cgtagtctat
    20221 ttttggttgg attatcgaca aatattgcca atgtgagtca acggatgatc gcagaagact
    20281 ttgaaatggt accagttgtt cgaagcaatc aaactttgct tggcgttgtg acgcgacgag
    20341 atgtcatgga gaagatgagc cgttcccaag tttcggctct accaactttt tctgagcaga
    20401 ttggacaaaa gctctcttat caccatgatg aagtagtcat tacagtggaa ccctttatgc
    20461 tagaaaaaaa tggagttttg gctaatggtg tattggcaga aattctgacc cacatgaccc
    20521 aagatttagt tgttaatagt ggtcgcaatc tcattatcga gcagatgctg atctactttt
    20581 tgcaggctgt tcagatagat gatatattgc gcattcaggc acggattatt catcatacga
    20641 gacggtcagc tataattgat tacgatattt atcatggtca ccagattgtt tcaaaagcaa
    20701 atgtgactgt taaaattaat tagaaactag gagaaaagat gataacatta aaatcagctc
    20761 gtgaaatcga agctatggac aaggctggtg attttctagc aagtattcat ataggcttac
    20821 gtgatttgat taagccaggc gtagatatgt gggaagttga agaatatgtc cgccgtcgtt
    20881 gtaaagaaga aaatttcctt ccacttcaga ttggggttga cggtgccatg atggactatc
    20941 cttatgctac ctgttgctct cttaacgatg aagtggctca cgctttccct cgtcattata
    21001 tcttgaaaga tggtgatttg ctcaaagttg atatggtttt gggaggtccc attgctaaat
    21061 ctgacctgaa tgtctcaaaa ttaaacttca acaatgttga acaaatgaaa aaatacactc
    21121 agagctattc tggtggttta gcagactcat gttgggctta tgctgttggt acaccgtccg
    21181 aagaagtcaa aaacttgatg gatgtaacca aagaagctat gtacaagggt attgagcaag
    21241 ctgttgttgg aaatcgtatc ggtgatatcg gtgcggctat tcaagaatac gctgaaagtc
    21301 gtggttacgg tgtagtgcgt gatttggttg gtcatggtgt tggcccaact atgcacgaag
    21361 aaccaatggt tcctaattat ggtattgcag gtcgtggact ccgtcttcgt gaaggaatgg
    21421 tcttaaccat tgaaccaatg atcaatacag gcgattggga aattgataca gatatgaaaa
    21481 ctggttgggc gcataagacc attgacggtg gattgtcatg tcagtatgaa caccaatttg
    21541 tcattacgaa agatggacct gttatcttga ctagccaagg tgaagaagga acttattaat
    21601 aaaaagtgaa aagactactg gaagtttatt ttgataaaaa atccagtaga tcttttcata
    21661 ataaaacgca ttgtatcaag tgttaggggc tgatatcatg cgtttttctg cttttaagat
    21721 tttttccaac tctgtttgta agcgcatcat aacaaagggt ctaggattca gggctctcct
    21781 cctatatact attagtaaag taaaactaag ggaggatatt ttagtgtcgc agtctattgt
    21841 tcctgtagag attccacaat attgtcgttt tgattctaaa aagagaaatg gaattctgtt
    21901 taatgttcgt attgccaatc ttaaatttac ttttttatat ta
//
LOCUS       DAVGJJ010000028        21272 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000028 DAVGJJ010000000
VERSION     DAVGJJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.028"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1074
                     /locus_tag="VVE35_000960"
                     /pseudo
     CDS             1..1074
                     /locus_tag="VVE35_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1185..1442)
                     /locus_tag="VVE35_000961"
     CDS             complement(1185..1442)
                     /locus_tag="VVE35_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU3061175.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(1484..3520)
                     /locus_tag="VVE35_000962"
     CDS             complement(1484..3520)
                     /locus_tag="VVE35_000962"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU3061176.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYITKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEEEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(3732..4649)
                     /gene="glyQ"
                     /locus_tag="VVE35_000963"
     CDS             complement(3732..4649)
                     /gene="glyQ"
                     /locus_tag="VVE35_000963"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU3061177.1"
                     /translation="MSKKLTFQEIILTLQRFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKHLGYPLLDEETRAKLLAEDAE"
     gene            complement(4843..5169)
                     /locus_tag="VVE35_000964"
     CDS             complement(4843..5169)
                     /locus_tag="VVE35_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU3061178.1"
                     /translation="MVEYISRKYFLTEEEFQAEKICNQSLVELFQKKYSWHSLNDFGS
                     STYDKNYASIFYEYWRSFLTVDKLVENLGSVQAVLDSYHLWANTEKTFPLLDWFVQQK
                     LIEKEI"
     gene            complement(5136..5420)
                     /locus_tag="VVE35_000965"
     CDS             complement(5136..5420)
                     /locus_tag="VVE35_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU3061179.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMTLISGSKRGWLNILVASIS"
     gene            complement(5693..6535)
                     /locus_tag="VVE35_000966"
     CDS             complement(5693..6535)
                     /locus_tag="VVE35_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU3061180.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSITTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(6649..8040)
                     /locus_tag="VVE35_000967"
     CDS             complement(6649..8040)
                     /locus_tag="VVE35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU3061181.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(8275..8652)
                     /locus_tag="VVE35_000968"
     CDS             complement(8275..8652)
                     /locus_tag="VVE35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU3061182.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKHIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            8885..9862
                     /locus_tag="VVE35_000969"
     CDS             8885..9862
                     /locus_tag="VVE35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU3061183.1"
                     /translation="MLKLGVIGTGAISHHFIEASHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEIFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            9859..10941
                     /locus_tag="VVE35_000970"
     CDS             9859..10941
                     /locus_tag="VVE35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3061184.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSKT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(11005..12050)
                     /locus_tag="VVE35_000971"
                     /pseudo
     CDS             complement(11005..12050)
                     /locus_tag="VVE35_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(12434..13630)
                     /gene="tuf"
                     /locus_tag="VVE35_000972"
     CDS             complement(12434..13630)
                     /gene="tuf"
                     /locus_tag="VVE35_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU3061185.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(14563..15432)
                     /locus_tag="VVE35_000973"
     CDS             complement(14563..15432)
                     /locus_tag="VVE35_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU3061186.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(15655..16176)
                     /locus_tag="VVE35_000974"
     CDS             complement(15655..16176)
                     /locus_tag="VVE35_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3061187.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(16134..16742)
                     /locus_tag="VVE35_000975"
     CDS             complement(16134..16742)
                     /locus_tag="VVE35_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU3061188.1"
                     /translation="MAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQ
                     LIGTSYNVTDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPT
                     TVTVTANYYKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTT
                     YTLVATPANAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(16730..17239)
                     /locus_tag="VVE35_000976"
     CDS             complement(16730..17239)
                     /locus_tag="VVE35_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061189.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKPQNWQN"
     gene            complement(17350..17604)
                     /locus_tag="VVE35_000977"
     CDS             complement(17350..17604)
                     /locus_tag="VVE35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061190.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(17944..18477)
                     /locus_tag="VVE35_000978"
     CDS             complement(17944..18477)
                     /locus_tag="VVE35_000978"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU3061191.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(18464..18679)
                     /locus_tag="VVE35_000979"
     CDS             complement(18464..18679)
                     /locus_tag="VVE35_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3061192.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(18646..18789)
                     /locus_tag="VVE35_000980"
     CDS             complement(18646..18789)
                     /locus_tag="VVE35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU3061193.1"
                     /translation="MAYSTDFKQRALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            19023..20741
                     /locus_tag="VVE35_000981"
     CDS             19023..20741
                     /locus_tag="VVE35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU3061194.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIETPFAVEVADVETEKASGLIEVIGEVVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            20852..21199
                     /locus_tag="VVE35_000982"
     CDS             20852..21199
                     /locus_tag="VVE35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU3061195.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
BASE COUNT         6202 a         4566 c         3835 g         6669 t
ORIGIN      
        1 aacgccgttt caagtgctat cactgttcaa aaatgatggt cgctgaaact tctatcgtca
       61 agaagaatca ccaaatccct cgtatcatca accaaaagat tgctcaaaag ttaattgaaa
      121 agatttctat gaccgatatt gcccatcagc tttccatctc aacttcaact gtcattcgaa
      181 agctcaatga cttccacttt gagtgtaatt ttagaaatct gcctgagatt atgtcttggg
      241 acgttgaaac agtccgggga gtgactgttt caatcgggag atggagatga gctttattgc
      301 gcaagatttt gaaaagctca atatcatcac tgttcttgaa ggtagaacac aagctatcat
      361 tcgaaatcac tttcttaaat atgatagagc cgtccgatgt caagtgaaaa ttattactat
      421 ggatatgttt agtccttact atgacttggc taaacagctt cgctttcaaa tttctaggct
      481 caggctgaaa cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc
      541 tttcacattg tacaacatct tagccgtgct atgagtcgtg tgcgtgttca aatcatgaat
      601 cagtttcatc gaaaatccca tgaatacaag gctatcaagc gttactggaa actcattcaa
      661 caggatagcc gtaaactgag tgataagcga ttttatcgtc ctacttttcg tatgtactta
      721 accaataaag agattttaaa caagcttttg agctattcag aagacttgaa acaccactat
      781 cagctctata ataaggaacc agagaaattt ttcggactta ttgaggacaa tcttaagcag
      841 gttcatcctc tttttcagac tgtctttaaa acctttctaa agaacaaaga gaaaatcgtc
      901 aacgcccttc aactacacta ttctaatgcc aaactggaag cgaccaataa tctcatcaaa
      961 cttatcaagc gcaatgcctt tggttttcga aactttgaaa acttcaaaaa acggattttt
     1021 atcgctctga atatcaaaaa agaaaggaca aaatttgtcc tttctcgagc ttagcttttt
     1081 ttcaacccac tacagttgac aaagagccaa aaagactagc aaacacaaac tgctagtctc
     1141 tttgttatcg tttcatctat ctttgttaaa aaaaagagta catattatga atttgggtca
     1201 tcaagactac ggccatgtaa tcctttttca cgttgtactt ggcgtagttt ttctggtgta
     1261 acatcgtttc cttcttcgtc cacaattttg attccttcaa tgtgatgacg aacagcgcga
     1321 cgataacctt cgatgtactc ctcacgtagt ttggcttgtt ccactttttc ttctagtgtt
     1381 aagccttctg tttttttctt tttagcaagc tcattgatac gagcaatttt tttcggatcc
     1441 atttcttctc ctctgtaata agataaagtc cgtttatcaa attttattta gtgttaattt
     1501 ggttaaaaca agcaaactta gctgctttct tggttagttg tgacaagatt gccaaacgat
     1561 tttgacggac agcctgatct tcagccatta ccatagtatt ttcaaagaaa gcatcaatga
     1621 ctgggctaag cgcaaagagt tgtttcaatt gctgacttgc aggtcctgat aaaacgagtg
     1681 tttctactgc ttctgccaaa gctttttctt ggtcattctc aaatagtgct gaatcaaccg
     1741 tagcaacccc ttctgccttc tcagccaggt taaaggcacg agaaagtgat tcaacagatg
     1801 gtttaaaatc ttcttccttg cttacttcta cgagagcact tgctgcttcc aacatatctg
     1861 ccacaacaaa gtttgaacct gcaagaactg cttccttgat atcttttgga gtagagccca
     1921 tcatcttatc aacacgagcc ttgataaagt ccataacctc tgctttattt tcataagtca
     1981 aactgtcaaa tttcaatgca taaaggctat caatcagctc atccatagca atgtgccaac
     2041 caaaggcatc caagatacga accacacctt gagttgcacg acgaagggca taagggtcat
     2101 tagaacctga tggaatcaat cctactgaga agaaactcaa aatcgtatcc aatttgtctg
     2161 caatggctag aactgcgccg accttgctct ctggaagttc tccttcagct gatgtaggca
     2221 tgtagtgttc acgaatagca gctgccaccg ctggagtttc accagcaaga agggtgtatt
     2281 tttcacccat aattccttgg agttcgtcaa attcaccaac catacctgtc aacaagtcaa
     2341 acttgtaaat ggctgctgca cgagcaaggt caactgtttc atccactgac aaaccagctt
     2401 tttctgccaa aagtacagtg atttgacccg tacgaatcat gtgttcacga agggaaccaa
     2461 tcttctcatg gaaggtgaca ttgtttaatt tttcaacaag atctgaaatc accaattttt
     2521 ggtcttcacg ccagaagaat tctccgtctt ccaagcgagc taccaagact ttttcatttc
     2581 ctttaatgac atttttcaaa cgctctgcgt ttccgttacg aacagaaatg aagtttggca
     2641 agagttttcc atcttgatca cgaacaacaa agtaacgctg gtgttccttc atagaagtca
     2701 ccaagacttc ttctggaact tcaaggtatt tagcatcaaa acttcccatg aaggcagttg
     2761 ggtattcaac caaattcaag acttcattca gcaaatccgc atcaatttcg atacgtacac
     2821 catgttttgc ctcaatttcc ttgatttggt caacaatcat ttgctcacgt tcacatggat
     2881 ctgcgattac aaactgctta cgaaggtctt cttcatagct caatgctgac tgaattttgg
     2941 tttctttccc caagaaacga tgaccacgac tcacacgacc tcccttgata tcaaggaaat
     3001 ccaaatcaaa ctcttcttca tctaaaagaa cagttaaagt gtgaacagga cgaatgtatt
     3061 caaagctatt tccagcccag tgcatgctga caggaaaagt cagtgacttc aagacatcca
     3121 caatgcctgg aacaatggct tcaactgctt gaccaatttc ttccttagtg atatagacat
     3181 attcttcacc cttgatttca cggaattcga tatcttcaac agtcaagcct tttccacgga
     3241 caaatccttg agctgctttg gtgaagtttc catcactatc caaggcaatt ttctttgctg
     3301 gtcccttgaa atcttctgtc aaatcagact gtttgtctga taaaccagtc acacgaacag
     3361 ccaaacgacg tggtgttgag aaggtttgaa tggcttcaaa agacaggcgt tttcccttga
     3421 ggaaggctgc cattttttcg cctagttgtt tttcacttgg tgtgacaaca taggctggta
     3481 attcttcaag accgagttct actaataagt tttttgtcat gttttttctc cgaaaagtat
     3541 ctttttattt tccagtttgc cttatgtgat tggcacgcaa gcaaccaact tcgttgtttc
     3601 attgctaaaa ttggagccaa aagtctccaa ttttgcctag tatccttagt ttcactaagg
     3661 ataccttcat cactgcggca actggctcag ggctcactct gttcgcccat tttcgtaatt
     3721 tgtcactctc tttattctgc gtcttctgct aggagtttag ctcttgtttc ttcatccaaa
     3781 agtgggtagc ctaggtgttt gcgttctgcg acaaaggttt tggctacgac acgggccaag
     3841 ttacggatac gagcgatata gcctgcacgc tctgttacag atacggcacc acgcgcgtca
     3901 agcagattaa aggtatgtga acatttgaga acatagtcat aggcagggtg taccaagcct
     3961 tcttctaatg cacgaccagc ttctttttca aacttatcaa agttttcaag caacatttct
     4021 tggtccgaaa tttcaaatga atattttgag tgctcatact caggctggat aaagatttct
     4081 ccgtatttta caccatcagc ccactcgata tcatagacag agtctacttc ttgaatgtaa
     4141 gaagccaagc gctccaaacc ataggtaact tccgcagtca cagggccagt tgccaatcca
     4201 ccgacttgtt ggaaataagt gaactgagtg atttccattc cgtcaagcca aacttcccaa
     4261 ccaagaccag ctgaaccagt tgatgggttt tcccagttgt cctcaacaaa acgaatatcg
     4321 tgctccaaag gattgattcc caatttttcc aaagactcaa ggtaaagttc ttggatattt
     4381 gatggagaag gcttcatgac cacctggaat tggtggtgtt ggtagagacg gttagggttt
     4441 tccccataac gaccgtcagc aggacgacgt gatggctcta cataagctgc attccatggc
     4501 tcaggtccga tagcacgaag gaaagtgtaa ggactcattg ttcccgcacc tttttcattg
     4561 tcataagcct gcataagcat acaaccttgg tcattccaaa atcgttgcaa agtcaaaata
     4621 atttcttgaa atgttaattt cttagacatt gaatactcct taataaaaaa taatttcttg
     4681 cgacacgagt ctacctcgtc gattatacga ggcaagacga gttagtactg cgtctagctc
     4741 agcaccctag tgctgagcgt ggggcagtcc gactgtaagg aggtctctgc cactgatgca
     4801 gtgaaatgtc aatttcttag acattgttta ctcctttagt ttttatattt ctttttcaat
     4861 taatttctgc tgaacaaacc aatctaacaa gggaaaagtt ttttctgtat ttgcccataa
     4921 atgataagaa tctaagaccg cttgtacact acctaaattt tctaccaact tatctactgt
     4981 caaaaagctg cgccagtatt cataaaaaat acttgcatag ttcttatcat aagtcgaaga
     5041 accaaaatca ttcaatgaat gccaactata cttcttctga aaaagttcta cgagagattg
     5101 attacaaatt ttttccgctt gaaattcctc ttctgtcaag aaatacttgc gactaatata
     5161 ttcaaccatc ccctcttcga accagatata agagtcataa ccattcgtct attatttgta
     5221 taggctggaa cagcgctttc cctaataatc tgtgtagcag cattaaaatc tgaccaaata
     5281 acggcttgcg gtaaatcaca gactgcaagt tcattcttta aaaaagattg ataatctttc
     5341 aacttttcag tatttcttac tacaaaatca cgaaatgtag ctagttgact atcgtctttt
     5401 acaagataaa ggttgtccac ggctctcctt tctttcacac aatagactga ataagcaaaa
     5461 gaaccacatc tgacaatcct aggttgattt aacctagggg cgtcagaaac gcggttccac
     5521 cctaatttat tacttcattg tttttgaaaa tactacagaa agcgccagcc ctttattttg
     5581 ccctacttgg ctcccactat cccaagctcg cttgaagaac gccataagac tttctttcct
     5641 gctgactatt atatcaaaaa ttagaataat gtacaattct ttttatgatt ttctagaaat
     5701 ccacatcatc aactcgtgga gcacccgatt gaacagcaat cgtttttaag gtttctctct
     5761 cctcatgact cagttcaatt ccaaagcaat caagattagc ttgaatacga gaggttgtga
     5821 tagattttgg aagtggtaaa aatccttctg ccaagctcca ggccaaggct atctgagcaa
     5881 tcgattttcc gtgatttgct gcgatttctt gcacttgctt gctatcaaat agttctccct
     5941 gaccaaaagg cccccaagct tccaataaaa ttcccttttc acgacagtaa gctacgactt
     6001 gatcttgata cacacctggc gccaagcgaa cttgattgac cgcaggaacg atagttgcag
     6061 tttcaagcaa ggcatccaaa tgatggggaa gaaaattgct aacgccgata gcacggattt
     6121 tcccttcttg atagaggtct tccatcgctc tccaaacttc cgcattgcga gttttccatg
     6181 cgtcattttc tctgagcggt tttgggttcg gccaatgaat caaatacaaa tccaaataat
     6241 ccaagcccag tttttctata gatttttcca aagcttgacg agtttgctca taggtttgct
     6301 gactattcca aagcttggta gttacgaaca tttcttcacg tggaactccg ctatctttga
     6361 ttgcttgacc aacactttct tcattctgat aaatcgccgc cgtatcaata tgacgataac
     6421 cagccttcaa ggcttctaac actgcacgat aggcttcttc tccatcctta gccttaaaag
     6481 ttccaaatcc caatactgga atttctactc cattatttaa ttgataggta ttcatatgct
     6541 tctccttcta ttttattcat caattatagc ataaaacatt ttttaagtag tactatcttc
     6601 tgtaaaaaat aagtctgtca aatatctaac agacctattt tttcttgctt attcatcacg
     6661 actatagtac agctcatgag cttttaggcg agtattgagc atctctggta tggtcacaaa
     6721 ggtataacct tgatttttca aatactcaat gacccttggc aaggcattga ctgtcggact
     6781 gtgaatatca tgcatcaaaa cgatagagcc gttagctact tgatgctgaa tttctgtcaa
     6841 aatagatgct tcatttttac tcttccagtc cagactatcc acatcccaca tgataaagct
     6901 caaatccaag ctattgcgaa tatcatctgt aatagcacca taaggtggac gcatgagttt
     6961 agaactagaa cccagcactt tagttagcac atcctcagta tcagtaatct gctttttagc
     7021 ttcatcaaga gagagttgcg agagaatcgg atggctccaa ctatggtttc caacaacatg
     7081 accttcagat tttatcctct tcaccaagtc ctcattccca gaaacatttt tcccaagcac
     7141 aaagaaagtc gctttaatat catatttagc tagggtctct aatacctgcg gggtcgttgc
     7201 tggatttgga ccatcatcaa aggttagagc gacaactttt tgatatttct tatcaaagta
     7261 agattggtac aaggccgcat ctttttcgag taagtacgaa gattggataa catcaaagaa
     7321 agcagatact ggcaaggcta tctcttctaa attttcaacc acaggacttg gataaaggat
     7381 aatctgacta tccttgtaat caaaattcca tgcagacaag tcttggtcag agaagttttt
     7441 tacaatctgc tcactctggt cttgctctat ttttttatcc tctatgaagg aggtcaactc
     7501 ttttatcagc tgttccttag ccttactagc atctgaaaac agttggtcaa gtgtaaaagg
     7561 ttgcccatct tctgttaagt gaacctttcc tagactggtc ttttcagtct cttcaatctt
     7621 aaaagcaact aaatcataga tttgtttggt cacattacga ttaacgactc cctttaaacc
     7681 tgactcttct tgctctgtat agtagaaaac aagattgtcc ttactttcta gcttgtcctt
     7741 aacatcttga tttatcagct ccctaacaga ggaaatcact ttctcccctt ggagaggata
     7801 ataggcaatc acttcggctt gcccctgacg aaaatgctcc ttctgatttc cctcactcaa
     7861 ttgatcatct ttctcttttt tgagcgattc aatcttttgc tcaaaacttt tttgctggta
     7921 gatcttaaaa gcaataaatc cgcctaatag gcaaatagaa attgctaaga taccaatcaa
     7981 agccaataat acatgtctcg ttttcccgtg tctgccacgt cctagtctac ttttattcat
     8041 atttatatca taacaaattt gagcaataat ttcaatgata caatttggca atccacaatt
     8101 cctctagcaa gccaaagcat cttacatcaa ctccaacatc cgtatctgta gagattatgt
     8161 agttttctat ctatggaatt tctagtagat tgaagctaga atagtagacc tatacttcta
     8221 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttct catttcattt
     8281 tactataaaa ctatgaaaag ctaggtcatc tgagaagtaa ttactatcga caattgcgat
     8341 agcgtatact ggtgcgatgt gtttgtagct ctggtgggta tcaccttcac gttggcgaat
     8401 tttttctagg ttttgattaa cctgactgca cagataagcc cataggcgat tgatgaaaaa
     8461 attctgatga tgaacctgaa tttcgatgat aacttggatt ccattatcta actccgccaa
     8521 gacgtctata ctagtataga aatcctgcgc tgagtagggc atggaaggca agacgtgaat
     8581 gttacttccc tccaaaatcg tcacattttt tgctggcaag tccagcatat cgcggataaa
     8641 ttgacaaatc attagttggg ctgatacccg gatgtctgag aatcctcctt ttcctccttc
     8701 tattatagca catttttaaa tctaggtttg ctagattcta tgcttctatc tatttattcg
     8761 gaaaagaaag cttttcttca gaagaaagct aatagcttat ttgaaatttt ctcctctctg
     8821 ttggatagtc ccgtctatct atgttataat gaaagaagga tttaaaatcg gaaaaggagt
     8881 atttatgcta aaattaggtg tcatcggaac tggcgctatc agccatcatt tcatagaagc
     8941 atcccatacc agtggagaat accaactggt cgcaatctat tctagaaaac tagaaactgc
     9001 agcaaccttt gcttctcgct atcaaaatat ccaactattc gatcaattag aaatcttctt
     9061 caaatcttct tttgatttag tctatatcgc cagtccaaac tccttacatt ttgctcaggc
     9121 aaaagctgcc ttgtctgctg gtaaacatgt tattcttgaa aagccagctg tcactcaacc
     9181 acaagaatgg tttgatttga ttcaaacagc tgaaaaaaat aactgtttta tctttgaagc
     9241 agctcgtaat tatcacgaga aagcctttac tactatcaaa aactttttag cagataagca
     9301 agtgttagga gcagatttca actatgccaa atactcttcc aagatgcctg acttgttggc
     9361 tggacaaaca ccaaatgtct tttcagaccg ttttgctggt ggagccctca tggatttggg
     9421 gatttatccc ctctatgctg ctgttcgtct ttttggaaaa gccaatgacg cgacttacca
     9481 tgctcaacag cttgacaata gcattgacct aaatggagat ggtatcctct tctacccaga
     9541 ctatcaagtt cacatcaagg ctggaaaaaa catcacttcc aatcttcctt gcgagattta
     9601 tacaacagat ggaaccttga ctctcaacac gattgagcat attcgctcag ctatttttac
     9661 cgaccaccaa ggaaatcaag tccagcttcc tatccaacag gctcctcata cgatgactga
     9721 ggaagtcgct gcatttgcac acatgatcca gcaaccagat ctgaatctct accaaacttg
     9781 gctgtatgat gcaggctctg ttcatgagct actatatacc atgcgccaga ctgctggcat
     9841 tagatttgag gcagaaaaat gaaaaccaaa ctaccgactg aatggcaaga actgagtgac
     9901 caactcggtt tccaagaatt cacccccatt caaactcaac tatttgagcc ccttcttgct
     9961 ggagaaaacc tcctaggagt gagcaaaacc ggaactggta agaccctagc gtatctccta
    10021 ccaagtcttc tcagactaca aaagaaaaaa gcccaacaac tcttgattct agcaccaaat
    10081 acagaacttg ctggacagat ttttgatgta tgtaaaacgt gggcagaagc tatcggattg
    10141 actgctcaac tcttcctttc aggttcaagt cagaaacgac aaattgaacg tcttaaaaag
    10201 gggccagaaa ttctgattgg aactcctggc cgtatctttg aactcattaa acttaaaaaa
    10261 atcaagatga tgaatgtgga aaccatcatc ctggatgaat ttgaccaatt actagatgat
    10321 tctcagattc actttgtaga aaaaatcact cactacgcac ctcgtgacca ccaactggtc
    10381 tacatgagtg ccacgaccaa gtttgaccaa gaaaagattg tgccgaatac acgcactatt
    10441 gatctctctg atcaaaaatt ggacaatatc cagcatttct acatgcaggt agaccaacgt
    10501 catcgagtgg atatgctacg aaaactggct catgtagagg atttccgtgg tctagtcttc
    10561 ttcaatagcc tgtcagacct tgggaatgcc gaggaaaaac tacagtatcg tgatatattg
    10621 gctgtttccc tcgctagtga tgttaatgtt aaatttagaa aagtcatctt agaaaagttt
    10681 aaagataacc aactaaccct gcttcttgca actgaccttt tggctcgtgg aattgatatc
    10741 gatagcctag aatgtgtcgt aaactttgat attcctagag atagcgaaac ctacacacac
    10801 cgtgctggcc gtactggccg tatgggcaag gaaggatatg ttattactct cgtcactcat
    10861 ccagaagaaa tcaaaaaact taaaaagttt gcaagtatcc gagaaattgt cctaaaaaac
    10921 caagaactct atatcaaata aagccccatt gctctataca catccagtgt atggagcttt
    10981 tttataagca attcaatttt atactcatct gctttcaaaa agcattctag tccatctccg
    11041 attaacgatg gactttatca cctccttctc cagtccttgt ataacatctt ggagttgatt
    11101 catgacatct tccaaagttc gaaaggcttt attcttaaat ccacgtttac gaatctcttt
    11161 caacacttgt tcaatgggtt catctctggt gtgtatggag gaataaatgc aaagccaata
    11221 ttagtcggaa tctttaaggc acttgattta tgccatatag cattgtccat aacgagtaaa
    11281 aaataatcat ctggataagc ttgtgaaagc tcttctaaaa agacgttcat ccactcagta
    11341 ttacatccac cagctattaa gaaaactgat gcgcctgtat gggcatcaac agctccataa
    11401 caatagcgaa attctcgtat atagtgacta tggatatgtg gacctactcc tattggagcc
    11461 caacaagatc ccagtttact gattctaccg aaaccagctt catcttggta catcaagcga
    11521 accttacgaa aacgtctact ggttttaaag cgctttcttg tcttcttgaa ttgagatttt
    11581 atttttagac gcgacaatgg tttgagcgtc tgctttctta ggatgttctg gacgtggcat
    11641 aatatttcgc caaccatggc gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg
    11701 acctaactcc tttttataag cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc
    11761 tgcagccttc aaatggcggg caagaaaggc tttttcttcc tcaactgtca tatatgatgg
    11821 ttacgaccac cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atattttttt
    11881 acatttcgcc aaatcgttgt ttgattacag tctaaaagct ctataatctc tttataagat
    11941 ttgcccatca gacgaaatag aacgatttga aggcgtttat gatatttagc tgtacgagag
    12001 tcttttaaaa gtgttttgat ggtttggatt tcttctttaa ttgatttcat attactattg
    12061 tataatgctt tttgatttta ggctagtatt atactcttcg aaaatctctt caaaccgcgt
    12121 cagcttcgcc ttgtcgtata gatgttactg acttcgtcag ttctatctac aacctaaaaa
    12181 cagtgttttg agcagtctgt aactaacttt ctatagtaaa atgaaataag aacaagacaa
    12241 atcgatcagg acagtcaaat tgatttctaa caatgtttta gaagtagatg tgtactattc
    12301 tagtttcaat ctattatagt ttactctttg attttcattg agtattagtt caatatctaa
    12361 aaaaagaacc ttgccaagca agattctttt agtgtctgac ttaaataatt gttcttctgg
    12421 gaactaaatc gaattaagct tcgatttctg taaccatacc tgaaccaaca gtacgtccac
    12481 cctcacggat agagaatgta gtaccttgtt ctacggcgat tgggtgaatc aactcaacgt
    12541 cgattgtcac gttatcacca ggcattacca tttcagtacc tgctggaagt tcgattgaac
    12601 ctgtaacgtc agtagtacgg aagtagaatt gtggacggta gttgttgaag aatggagtgt
    12661 gacgtccacc ttcttcttta gtaaggatgt agacttcacc tttgaattta gtgtgtgggt
    12721 tgattgaacc tggtttagcg ataacttgtc cacgttcgat ttcatcacgt tgaacaccac
    12781 gaagaaggac acctacgtta tctccagcaa gaccttcgtc aagttgttta cggaacattt
    12841 caacaccagt aacaactgct ttttgagttt cttctttgat accaacgatt tcgatttcgt
    12901 cgttgacttt aacgatacca cggtcgatac gtcctgaagc aactgtacca cgtccagtga
    12961 ttgagaatac gtcctcgact ggaagaagca atggtttgtc agtgtcacgt tctggttctg
    13021 ggatatactc atcaactgtg ttcatcaatt ccataacgat gtcttcgtat ttagagtcac
    13081 cttcaagagc tttaagtgct gaaccttgga taactggaag atcgtcacct gggaagtcgt
    13141 attctgacaa taggtcacgg atttccattt caaccaattc aagcaattct tcgtcgtcaa
    13201 ccaagtcaac tttgttcatg aagacgataa ggtgtttaac accaacctga cgtgaaagaa
    13261 ggatgtgctc acgagtttgt ggcattggtc cgtcagttga agctactaca aggatagctc
    13321 cgtccatttg agcagcacca gtgatcatgt ttttaacgta gtccgcgtgt cctggagcgt
    13381 cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac gtgcgcagtg ttgatagtga
    13441 taccgcgttc gcgttcttct ggagcagcat cgatagacgc atagtcttta ggttggttaa
    13501 ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc tgcagttagg gtagttttac
    13561 cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg tttactacga tcgtattttt
    13621 cttttgccat ttgagtaaaa gcctccaata aaatatattt tatagataga cagtaggcaa
    13681 tacagtctaa ctttccttac tattttatca aatttcagct aaattgcaag tgttttacaa
    13741 cgtttttgtg aattattctt ggtaaaaatt taactaacta atagaaagta ctaaatagac
    13801 ttatccttca aggagttttc agcattatcc atccactcac ttgctgaaaa tgtaaacagt
    13861 ctctggttta tatagtaaaa tgaaacaaga atagggcaaa tcgatcagga cagtcaaatc
    13921 gatttctaac aatgttttag aagttgaggt gtactattct agtttcaatc tactatacaa
    13981 cctcaaaact gtgttttgaa caacctgcgg ctagcttctt agtttgccct ttgattttca
    14041 ttgagtataa aattgcagat aaaacgataa caatcatagg actcagcaga gtcaatgtag
    14101 gtcgaagaat agccccccta atcgcttatt ttatagcaaa atgaaataga aactgaacat
    14161 tttgttgagg aaggtaaatc aattcctcac aatgtttgag aaagaaggga ggactattcc
    14221 aacttcaacc cactataaag cagacttaca agatagggaa cccatctccg ataatattcc
    14281 gataatatat agtagaatga aaactgcaca atttgattgg agaattcaaa ccaatttcta
    14341 acaatgtttt agaaacaagg gtctattatt tcaactgcaa cctactatag cccattgaag
    14401 ctggaatagc ccactgagac ttctaaacca ctgctggaga ggagtttgtt tagagtttgt
    14461 ttagagtttc tttcatttcc ctaaaaaatc aaaaagagag aaccagtctg atcctctctt
    14521 acttatagat ggtgctcact caaatgttaa aggagctatt tcttagagat aaaggaattt
    14581 gaatacagct actgccgcaa ttgctgctgc gataggtgct actactggta cccaagaata
    14641 ccaccatttt gaatcgcctt tatgctcacc aagaactgat tttggaagga aagcatgaag
    14701 gagacgtggt cccaagtcac gggctgggtt caaggcaggt cctgtaggtc ctccaagtga
    14761 tgttaccaaa gccataacga ggaatccaag tgccaagtga gccacagaaa gtcctgaagc
    14821 agtgtgtgga gccacctgtg ctttaatagc caagtcagaa aaatcaactg tttgtcctgc
    14881 ttctattgcc ttttgtttca tgaattgaag cacttcagca ccaaagaagt ttttagtcaa
    14941 accaagagct gcaaagaaca aaacaaatga accaacaaac tcattaatca aaccattgac
    15001 agttgctgca tagcgacttt cttttgtacc atggtcaata cttgaaatag ttgagaaagt
    15061 tcccaagatg ttatttggtt tttcagtttt caagtagaat ggacggtatg ttgccacaac
    15121 taaggcttgg ccaaagatag cccccaagac ttgcgcgata atgtaaggta ccacttgtgc
    15181 ccaagggaaa agaccgctaa ctgcaagccc tagagtgaaa gcagggttga tgtgattccc
    15241 agatacgtta ccaaacatca aggctgggat cataaccccc ataccataac caacagcgat
    15301 gacgatccag ccactttggt gacctttcgt acctttaagt tcaacgttgg caactgcacc
    15361 attcccaaga atgatcaaaa tggcagttcc caaaaattca gtggcatact tcagtgccca
    15421 tgtgaaatcc atttgctaga ttctccttaa aattttttag atattcctta ttttatcaat
    15481 ttttacccta tttagcaact gatttctgga aatattttta aaaaaatagg attcatagtt
    15541 tcccagttca acgtacttgt aagcgttttt attcttcacg aaagacctca ttctcaaaac
    15601 caggaaaaag cccacatctg tgatcttttc cttgttccta gattctaaaa tctattattt
    15661 ttcatcttct tttttcttga agaatcctaa tcctagacca gctaacaagg tcatgatacc
    15721 agcactagcc aaggtagcat tagcttctgt acctgtgttt ggcaattcct ctctcttacc
    15781 tgtctcataa gtcggaactt ctgggtctgg attcactgga gtttcagttt ttggagtacc
    15841 tggttctgga gttggtttat ctggtgttga taaacggtca taccttaccg ttatttcttt
    15901 atcactagag tctgacgtaa cttcttgtga ttcaactgtt ggaatatctg gatctttgta
    15961 cttgtcaatc ttaccagata taacctcgtc ccagtttcct gttgtccatt caccataggt
    16021 tacaactccc gtgaccttgt tctcagtttt tgtacggctt aaggttacag gttgaacaac
    16081 atcttctttt acattttggt tcgtaacttt atcaacgtaa tgaatgatac gcgttatagt
    16141 cttcgtctca gtagaggttg ctgttttggg aaccactgtt tcctcaacat tctcacggta
    16201 gtaatagtca actgttgcac cgtcttctgg tacgcatttg caggagttgc taccaaggtg
    16261 tatgttgttt tccttgtgat aactcggtct tctttgtcct cagttgttgt tttcccttca
    16321 atagtttttg attctgtggt atactcagaa cctatcgcta aatcagcttt tataacagac
    16381 tctgccaact tctcttggct accttcttta tagtaattgg ctgttactgt aacagtggtt
    16441 ggcgcttcgc tttctctata aactaaggtc actgttctac cttcgcttac aatattccca
    16501 gttaaacttg cagaatttgt atctacttct ttaaaagtat aatattttcc gtcagtagta
    16561 gtcatgctac tgagtttttt atctgtgaca ttatagctgg taccaatcag ttgtttttta
    16621 ttggtaatgt aggttccgtc actttctttt tctccaattc cagtatcatt tttcaatgat
    16681 agcaaacgcc cttgttcatc aacatagcga actttcacat tttctgagat tagttctgcc
    16741 aattctgagg ttttttcttt ttcttgattt cttcggttat tttccctttc tcttcttcgg
    16801 gaatatttag ttttggaatg attttttcaa caacggttcg tgatggttcc acagtatctt
    16861 ggatgactga aaagtcagct agaattggga gattatactg aacacggtga ctttgagtgt
    16921 ttactcctac tctttcatta ttctctgaaa atacttgtac ggtataagaa acaacatctt
    16981 ttcctaatag aacatcccca gtagagaaat agccgccttt tcctagtttg ctatctccag
    17041 agcccacttc tttcctaatc ttatcagata gttttttacc agtcagtaca ttcgttcgca
    17101 caatcccttt gtctacccct acaaagtggg agaacttttt gaactcttca gaaccagatc
    17161 tagcccaacc attattaagg gcatttgctt ttgtatttgt attctctctc aaaagtttgg
    17221 cgattagaat tatattcatc ggcacttaga gttgctgcta tatctgactc ttgaatacca
    17281 acttccttac taccatttct agcggcagta tatgtgtatt tatctgtttc tacttctagc
    17341 tgattatcct taacatgata ggtaattttc tcctggtata agagtcttca gtagtttata
    17401 tgctctgtca tcatctttag ctttagaagc agctgagttt agccaagttg gggtattagg
    17461 agtatcttta tcagcctgtt tcaagacatt attcaagtca tttgctagcc tattggttgt
    17521 cctcactgtg aaagttttct tccagtctga accactttta ttagacttag atatgctcat
    17581 gaaatcctgg tcaacattca acacaatatc gccttgtttt ttagcatctt ccgttgcttc
    17641 tgcttcaaat tgatcatgga gtttttgttg ctctggtgta tagttttttt atttacacga
    17701 tttaatgtac tattttttat tttagtatta agaactccat taatacgatt tttaaactct
    17761 gcttcaaaaa atcccttaat ttgttggtgc gcaagttcaa cattcaaacc tttcttatac
    17821 aagtcttcat tttgaacgat ttcggctaca cggtgaatgg tttgattgta taccgtcttc
    17881 tcttaaccag cagtatcctt agttttaatg actactcgat aggttccttt agaaccgtta
    17941 tagtcatcta gttttaaaaa agcattccaa acattcaaaa atagttggaa cttcccttaa
    18001 taaatccgtc actttctttt tcaagatagc ccaagcttgc tcaataggat tcaaatccgg
    18061 tgaataaggt ggtagaggta agaaaaagtg tttatcttgg atgcagagtt catccaaaat
    18121 gttcttggga tgaaaactag cattgcccat gacaataaca tgaggtgtct tcaaagcagg
    18181 taggagttgc gttttgaacc actccacaaa gaaatcgctt gtcatgcttt tcttgtaaat
    18241 catgggagct ataaactctc cgtctacttg tcctgcaaca attgaagttc gctcaaaacg
    18301 acgtccgcta atcttttcat agactttctc ccctctagga gctcctgcat aaggacgata
    18361 gaggtagcgg tcgattcctg tttcatcaat atatacgatt ggtaaatctt ttaggttatc
    18421 caaaatatca agaaactcag cgactttctc tggctcttgt tccttaaagc tcgtcatctt
    18481 tttttaaagt gaccttaatc tgctttaaag ctgcccatac tgaaggaaca gcacaatcaa
    18541 aatgtgccgc aatttcccgt aaaaaagcat ctggatgagc ctctacaaag gctttcaatt
    18601 cctctaaagg aatctttcgg tttttgacga ctcgcttttt cctctctatg tgtccttgtt
    18661 cacgtctttc ttttcccacg tgaagagagt tctgacgcca acaccaaaaa acttggctgc
    18721 ctcgacatga ctgtgcccct ctttgatgga atctaatgct cgctgtttaa aatcagtact
    18781 atatgccata gctttattat aacatgtatt ttataaacta agctactata tcaatgtttt
    18841 gttataagaa gacttacttt ttttactgaa attgaatttt tcctagcctc aacatcatat
    18901 aaccccaaaa ttcaatctga cctggtttga agagatgttc gaagcagata aggttttact
    18961 aaaactaaaa aaaatgatat aatcaaactg attaaatgtt ttaaaaataa aggagaattt
    19021 gaatgtctta ccaagaaaat taccagaaat gggttgattt tgtggagctt cctgactacc
    19081 ttcgtcaaga tttggaaaat atggacgaaa aaactaagga agatgccttc tatacaaatc
    19141 ttgaatttgg tactgcaggt atgcgtggct tggttggtgc tggtacaaac cgtatcaaca
    19201 tctacgttgt tcgccaagct actgaaggat tggctcgttt gattgagtca aaaggtggaa
    19261 acgagaaaga acgcggtgta gcaattgcct acgatagccg tcacttctca cctgagtttg
    19321 cctttgaatc tgcggcggtt cttgctaaac acggcatcaa atcttacgta tttgaaagcc
    19381 ttcgtccaac tccagaacta tcatttgcag ttcgtcatct caactgtttc gcaggtatca
    19441 tggtcacagc cagccacaac cctgctccat ttaacggtta caaggtttac ggtgaagacg
    19501 gtggacaaat gcctccacac gatgcggacg ctttgactac ttatatccgt gcaatcgaaa
    19561 ccccatttgc agttgaagtt gctgatgtgg aaactgaaaa agcttctggc ttgattgaag
    19621 ttatcggcga agttgttgac atagaatacc ttaaagaggt taaggacata aacatcaacc
    19681 cagccttgat tgaagaattt ggtaaagaca tgaagattgt ctacacacca cttcatggta
    19741 ctggtgaaat gttggctcgt cgtgctcttg cccaagcagg atttgactct gttcaagttg
    19801 ttgaagcgca agcaactgct gacccagact tctcaactgt aacatcgcca aacccagaaa
    19861 gccaagcagc ctttgccctt gctgaagaac ttggtcgtca agttggtgca gatgttcttg
    19921 tcgcaactga cccagacgct gaccgtgttg gtgttgaagt tcttcaaaaa gatggtagct
    19981 acctcaacct ttcaggtaac caaatcggtg ctatcatggc taaatacatc ttggaagctc
    20041 acaaaaacgc tggaactctt cctgaaaatg ccgctctctg caaatctatc gtttcaactg
    20101 acttggtaac gaagattgct gaaagctacg gcgcaactat gttcaacgtc ttgacaggtt
    20161 tcaaatttat cgctgaaaaa atccaagaat tcgaagaaaa acacaaccac acctacatga
    20221 tgggatttga agaaagcttc ggttacttga ttaaaccatt tgtacgtgat aaagatgcca
    20281 tccaagccgt tcttgtcgtt gctgaacttg ctgcctacta ccgttctcgt ggtttgacac
    20341 ttgctgacgg tatcgaagaa atctataaag aatatggcta ctacgcagaa aaaacaatct
    20401 ctgttactct ttcaggtgtc gatggtgctg aacaaatcaa agcgattatg gctaaattcc
    20461 gcaacaatgc tccaaaagaa tggaacgcaa cagctatcac tgtcgtagaa gacttcaagg
    20521 cacaaactgc tactgttgct gacggtactg ttacaaactt gacaactcct ccaagtgatg
    20581 tgttgaaata cacacttgct gacggttcat ggattgccgt tcgcccttca ggtacagaac
    20641 caaaaatcaa gttctacatt gcagttgtag gggaaaccaa cgaagaatca caagctaaga
    20701 ttgctaacat cgaagcagaa atcaatgcat ttgtaaaata aagacctata aaagatgaga
    20761 ccaaccaacc tcatcttttt tcgtatcaaa tctaagcaat tttagaaact ttatggcata
    20821 ctagacttat caatgaaagc gaggtaatct catggaacaa tttttagata acatcaaaga
    20881 ccttgaagtc actacagttg tgcgtgcgca agaagctctt gataaaaaag aaactgcaac
    20941 cttctttatc ggtcgcaaaa cttgccctta ctgccgtaaa tttgcaggta cattgtcagg
    21001 tgtcgtagct gaaaccaaag ctcacattta cttcatcaat agtgaagaac caagccaact
    21061 caatgatttg caagcattcc gctcacgcta tggaatccca actgtaccag gttttgttca
    21121 tattacagat ggacaaatca acgtccgttg cgactcttca atgtcagcac aagaaatcaa
    21181 agatttcgca ggattgtaaa aaatcagcta acaagtctta aaatcacaaa aacgcataat
    21241 gtaaggtgca aaaaccttga tattatgcgt tt
//
LOCUS       DAVGJJ010000029        20970 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000029 DAVGJJ010000000
VERSION     DAVGJJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.029"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..624
                     /locus_tag="VVE35_000983"
                     /pseudo
     CDS             1..624
                     /locus_tag="VVE35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(635..787)
                     /locus_tag="VVE35_000984"
     CDS             complement(635..787)
                     /locus_tag="VVE35_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU3061196.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDY"
     gene            complement(946..2553)
                     /locus_tag="VVE35_000985"
     CDS             complement(946..2553)
                     /locus_tag="VVE35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU3061197.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMGQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLAQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2706..4190
                     /locus_tag="VVE35_000986"
     CDS             2706..4190
                     /locus_tag="VVE35_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU3061198.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAIKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAAHAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4285..4767
                     /locus_tag="VVE35_000987"
     CDS             4285..4767
                     /locus_tag="VVE35_000987"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU3061199.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(5062..7167)
                     /locus_tag="VVE35_000988"
     CDS             complement(5062..7167)
                     /locus_tag="VVE35_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3061200.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGSEVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EVGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
     gene            complement(7423..7563)
                     /locus_tag="VVE35_000989"
     CDS             complement(7423..7563)
                     /locus_tag="VVE35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061201.1"
                     /translation="MADRTDEVSSKHGFEVVDKAKLMWFEEVFEECKKYWSVKVKTLD
                     LF"
     gene            complement(7775..8755)
                     /locus_tag="VVE35_000990"
     CDS             complement(7775..8755)
                     /locus_tag="VVE35_000990"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU3061202.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(8757..11009)
                     /gene="pbpX"
                     /locus_tag="VVE35_000991"
     CDS             complement(8757..11009)
                     /gene="pbpX"
                     /locus_tag="VVE35_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU3061203.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(11013..11330)
                     /gene="ftsL"
                     /locus_tag="VVE35_000992"
     CDS             complement(11013..11330)
                     /gene="ftsL"
                     /locus_tag="VVE35_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU3061204.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(11342..12292)
                     /gene="rsmH"
                     /locus_tag="VVE35_000993"
     CDS             complement(11342..12292)
                     /gene="rsmH"
                     /locus_tag="VVE35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU3061205.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDVLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            12458..12652
                     /locus_tag="VVE35_000994"
     CDS             12458..12652
                     /locus_tag="VVE35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061206.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            12668..13210
                     /locus_tag="VVE35_000995"
     CDS             12668..13210
                     /locus_tag="VVE35_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU3061207.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            13221..13463
                     /locus_tag="VVE35_000996"
     CDS             13221..13463
                     /locus_tag="VVE35_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061208.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            13474..13809
                     /locus_tag="VVE35_000997"
     CDS             13474..13809
                     /locus_tag="VVE35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061209.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(13919..14920)
                     /locus_tag="VVE35_000998"
     CDS             complement(13919..14920)
                     /locus_tag="VVE35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3061210.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEDRNQEERQQVLDCSVNWKESTF"
     gene            complement(14980..16881)
                     /locus_tag="VVE35_000999"
     CDS             complement(14980..16881)
                     /locus_tag="VVE35_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU3061211.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQNQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIERVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(16903..17196)
                     /locus_tag="VVE35_001000"
     CDS             complement(16903..17196)
                     /locus_tag="VVE35_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU3061212.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(17196..18014)
                     /locus_tag="VVE35_001001"
     CDS             complement(17196..18014)
                     /locus_tag="VVE35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3061213.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(18001..18780)
                     /locus_tag="VVE35_001002"
     CDS             complement(18001..18780)
                     /locus_tag="VVE35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU3061214.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(18795..19286)
                     /locus_tag="VVE35_001003"
     CDS             complement(18795..19286)
                     /locus_tag="VVE35_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU3061215.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(19297..20487)
                     /locus_tag="VVE35_001004"
     CDS             complement(19297..20487)
                     /locus_tag="VVE35_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU3061216.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(20499..20933)
                     /locus_tag="VVE35_001005"
     CDS             complement(20499..20933)
                     /locus_tag="VVE35_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3061217.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         6119 a         4756 c         3782 g         6313 t
ORIGIN      
        1 cttatttgac tgaaatagct tctgactttg gctgtcatcc aactaccatc cactatgcgc
       61 tcaaagctat gggctacact cgaaaaaaag aaccacacct actatgaaca agacccagaa
      121 aaagtagcct tatttcttaa gaattttaat agattaaagc acctagcacc tgtttagatt
      181 gatgaaacag gattcgatac ttatttttat cgagaatatg gtcgctcatt aaaaggtcag
      241 ttaagaagag gcaaagtatc tggaagaaga tatcagagga tttctttggt tgcaggtcta
      301 acaaatggtg aattaatcgc tccaatgact tacgaagaga tgatgacgag cgactttttt
      361 gaagcatggt ttcagaagtt tctcttacca acattaacca caccatcggt tattattatg
      421 gataatgcaa gattccatag aatggggaag ctagaacttt tatgcgaaga gtttgggcat
      481 aaacttttac ctcttcctcc ctactcgcct gagtacattc ctattgagaa aacatgggct
      541 catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta tgaggctctt
      601 ttgtcttgtt cttgtttcaa ttgactatag cagattaata gtctaatagg taattaaatg
      661 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      721 aagcagctaa aagcaaagca gatgcaaaag tcagacctgc actaaggaca cgcttcttta
      781 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      841 aaagtttata aaccttggtt ataaaaaaca aaggatttct gtcttttata cagtcctccc
      901 ttgtttttat acgatttcaa ttttaaattt ttctgcaaaa aatatttata gtaattccac
      961 acagaaagca tcccatggaa ctaagatttg tttttcaaag acttcttgag ctagggtgtt
     1021 ttcaatcaag acagatttga cttttccttc tactgtcaag tcttgctctt cattggacaa
     1081 gttagccaca actaggaagc gacggtcgcc atccttacgt atataagcaa agaccttatc
     1141 agccgtatca agcaattcaa agtcagctcg aattagccag ctattctcct tgcgaatttg
     1201 gaccagtttc tgataggtat agaaaataga atctggattt gccagcgctt cttggacgtt
     1261 gatcatctcg taatttggat taactgccaa ccaaggttga cctgttgaga aaccagcgtt
     1321 tttgctctcg tcccattgca taggggtacg ggcattgtca cgtccaataa cacggatact
     1381 gtccatgatt tcttgcatcg gaacaccttt ttcaagagcc tcacgcgcat agttgagaga
     1441 ttcaatatct tctacttgag ccagtgtttc aaacggatag ttggtcatcc caatctcctc
     1501 accttggtag atataaggag ttcctctcat gagatgaagt aagattgcaa aggctttggc
     1561 agatttttcg cggtattctt ggtcatttcc ccagattgag acaatacgag ggaggtcatg
     1621 gttgttccag aagagggaat tccagccgtc ctcaactcct aactctgtct gccatttgtt
     1681 gaagatttct tttaacttag cgatattcag ctctttttga tagtgccatt taggctgacc
     1741 ttcctgatac tgaagaccga tatgttcaaa ctggaagacc atagacaatt cttgcccctt
     1801 tggatcagag tagagcttgg caatctctgg cgttgctccc caagtctccc ctactgtcaa
     1861 gagatcctta tctccaaagg tcgcctgatt catttccttg agatagggat ggagcatagg
     1921 accattattg actaccttct cgtcaggaat tttgccaatc atgtcaataa catccatacg
     1981 gaaaccacca atacctttat caatccagaa gttcatcatc tcataaattt tctggcgaag
     2041 tttttcattt tcccagttga gatccggctg tttcttgctg aaaaagtgga gatagtattg
     2101 acctgacttt tcatcgtatt cccaagcaga cccactaaag atagaatcta ggtcattggg
     2161 ttcatcgcgc cagatatagt agtctcgctc agggctatta ggattttcac aggcctctac
     2221 aaaccaggca tgctcatccg aggtatgatt gaccaccaag tccatgatga tacggatatc
     2281 acgcttctta gcttctgcaa tcagttgacc catatcctcc atggttccaa aaatcgttgc
     2341 aatcgcttga taatcagcaa tatcgtagcc attatcatcc atagggctgt cataaacggg
     2401 agaaagccaa attgctgtga ttcctagctt ggctagatag tccaacttac tggtaattcc
     2461 tggcaaatcg ccaattccat ctccattact atccataaaa ctctttggat agacttgata
     2521 gactacggca ttatgccacc atttttcttg catctgttta cctcattatt ttcataatct
     2581 atagtctatg atagcacttt ttaaaatttt gtgcaagcgt ttgcttaatc tttttaattt
     2641 cttttttagc tccgtagttt cctaacttcc aattttttat tataatagag tccagaggta
     2701 aaaaaatgaa aaaacaagct tttagttctg aacaatattt gaatttacaa cgcgaccata
     2761 ttttggagcg cattaaccaa tttgacggca agctctactt agagtttggc ggtaaaatgt
     2821 tagaagattt ccacgctgct cgtgtccttc ctggttatga acctgacaac aaaatcaagc
     2881 tcttgcaaga attgaaagag caggttgagg ttgtgattgc cattaatgct agcaacattg
     2941 aacattccaa agcacgtggc gacttaggca tttcttatga ccaagaagtt cttcgtttga
     3001 ttgacaaatt caatgaatta gggatttttg ttggttccgt tgtcattaca caatacgctg
     3061 gccaacccgc tgcagatgcc ttccgcaacc aacttgagaa aaacggaatt gattcttatc
     3121 ttcattatcc aatcaaagga tatccgacgg atatggatca catcatttcc ccagaaggca
     3181 tgggcaaaaa cgactacatc aaaaccagtc gcaacttgat tgtcgtaacc gctcctggac
     3241 ccggttctgg aaaattggca acgtgtatgt ccaatatgta ccacgaccaa atcaatggca
     3301 tcaagtctgg ctacgctaaa tttgaaacct tccctgtttg gaatcttccc cttcatcatc
     3361 cagttaactt ggcttatgaa gctgccacag ctgaccttga tgatgtcaac atgattgacc
     3421 ccttccatct tcaaacctat ggagaaacca ctgtcaacta caaccgtgat atcgaaatct
     3481 tcccagtgct caagcgcatg ttggaacgta ttctcggaaa atcaccatac gcttcaccga
     3541 cagatatggg tgtcaacatg gttggtttcg ctattacaga tgacgaggct gctgtcgaag
     3601 cttctaaaca agaaatcatc cgtcgttact atcaaacagt tcttgacttc aaagctgaaa
     3661 aagttggcga agctgccatc aagaaaattg agttgctcat gaacgacctc ggtatcacac
     3721 ctgcagaccg taaggttgct gtcgttgcgc gccaaaaagc agaagaaact ggtggaccag
     3781 ccctagcctt tgaattgcca aatggggaaa tcgtcactgg taagaactca gaactctttg
     3841 gccctacagc cgctgccttg atcaacgcca tcaaaaaatc agctgacatc gctaaagaag
     3901 taaaactaat cgagcctgaa gttgttaagc caatccaagg tcttaaaatc gatcatctcg
     3961 gtagccgcaa tccacgcctt cattcaaatg aaatcctgat tgcacttgct atcacagcta
     4021 cagaaaatcc tgatgctgcc cacgctatgg aagaactcgg caacctcaaa ggaagcgaag
     4081 cccactcaac catcatcttg actgatgaag acaagaatgt ccttcgtaaa ctgggtatca
     4141 acgtaacctt tgacccatac taccaatacg accgcttata tcgtaagtaa agatttcaac
     4201 ctctccagcc tcggagaggt tttctctctg tctcaggagc aacctttatg atataatgag
     4261 agaagaaaac tgaaaggatt taccatgtca aaagaagtta ttgtcgaaag ttttgaactt
     4321 gaccacacca ttgtcaaagc accctatgtt cgcttgattg gggaagaaac aggaccaaaa
     4381 ggagacatca tctccaatta tgatattcgc ttggtgcaac caaacgaaga ctctatccct
     4441 actgccggcc ttcacactat cgagcacctc ttagccaaac tcatccgtac ccgcattgac
     4501 ggcatgattg actgttcacc atttggttgc cgcacaggct tccacatgat tatgtgggga
     4561 cgtcacacca gtgctaaaat cgcagctgtt atcaaggatt cgctcaagga aatcgctgaa
     4621 actactactt gggaagatgt cccagggaca accatcgaat cttgcggaaa ctacaaggac
     4681 cacagcctct tctctgctaa agaatgggca aaactcatct tggaacaagg gatttcagat
     4741 gatgcctttg aacgtcatgt gatttaaaat cgagtagggg ataatctcta gcccctctca
     4801 caccaccgta cgtgctgttc ggcatacggc ggttcaacta acttttaacg catgtcgttc
     4861 aaggtaataa tccaaacacg aaaccaatcc acgtttttca aggactggtt ttgatatagc
     4921 acgtttaagt accgacttct gagctactat agtggattga aactagaata gtacacttct
     4981 actatacgac atgcccgtcg tacctcaaaa ggaaccagtt ttttcagctg gctccttctt
     5041 ttttatcctc aaaattctgt cttaggcttt ctcacgaata accaaaacgc catctttcat
     5101 atctgcttcc agatgtttag catctaaatg atccaagtgg aagtctgtca ccttatcacg
     5161 aatttcttgt tcaaccacgc gacggagagg acgaaccccc atgacttcgt cgtaaccttc
     5221 ttctgtgata taatctttag ccgcttgact gactaccaag tcaatgtctt tcttagccaa
     5281 ggtttggtta acttcagcca acatcaaatc tacaatctta gaaaggtctt ccttagtcaa
     5341 ttgtgagaac tcgatgactg cattaaagcg gttgaggaat tctggacgga agaagggttt
     5401 caaacggtcc atcaattctg gtttatccgc atcttctgtc aagttggctt catagccaaa
     5461 tccagcattt gaggtcgcaa taatgacagt gttcttgaag tttactgtat ttccttgacc
     5521 atctgtcaaa cgaccatcat ctagaacttg gaggagaagg gtaataactt gaggatcagc
     5581 cttttcaatt tcatccaaga gaatgataga gtatggattg cgacgaacac gttctgttaa
     5641 ggtattgcta ttgtcatcat aacccacata gcctgctgtt gtaccaatta gcttagaaac
     5701 agctgtgcgg tcactgtatt cagacatatc caaacggata atcgcatcct gggttccaaa
     5761 catatcgagt gccaattgct tagcaagctc cgtcttacca accccagtag accctacaaa
     5821 gaggaagctg ccgattgggc gatttccttc atcaaaacca gcacggttac gacggatagc
     5881 acgagctaca gcttctacgg ccttatcttg accaatcacc ttgtcttgca agcgatgagc
     5941 catatctttc aaacgttcga tatctgaagc tcccatttgc gatactggga tacctgtcat
     6001 tcgttccaca gattcagcca catcgttgac acttgcagtc actttcatat cttctgtgtg
     6061 gttttcgatt ttcttttcca attctgcaat gcgtgtttta tagtttagag ctgcttcaaa
     6121 atcttctgct tcaactgctt tttcttgctt gtctttttcc gtttcgattt ctcgttcaac
     6181 agcatgcaca tctgttactg gatgttgagc cgccaagtga gcagctgtta catcgacaag
     6241 gtcaatagcc ttatctggca agctacgttg aggaatgtat tgaacagaat aatccaccgc
     6301 tgctttcaag acttcgtctg gcaagatgac attgtgatgt tgttgataga ggtcacgaat
     6361 gccttgaaga attttaaaag tattctctgc tgaaggagca ttgaccttca cttcgttgaa
     6421 acgacgagca agagcagcat tcttcaagat ggtgttacgg tattcgtctt gagttgttgc
     6481 cccaatcact gtcaattctc cacgagagag agctggcttg agaatatccg caagtccttt
     6541 agaaccactg tctccaccag tgctaccagc accaagaatt tggtgaattt catcaaagaa
     6601 gaggataata ttccctgctt ctttcacttc attgactaag ttttggacat tttcttcaaa
     6661 gctaccacgg tattgagtac caacctcaag acctgagata tcaatagaaa taatttcctt
     6721 gttcttgata gcagcaggaa catctccgtt cacaatggct tgcgctagac cttcgacaac
     6781 tgctgtctta ccaacacctg catctccgac caaaacagga ttgttcttgg tgcggcgtga
     6841 gaggatttca gatgtttctt gaatttcctt gtttcgtccg ataacaggat ccaacttgcc
     6901 ctcacgcgct tctgctgtca agtttcgacc tagttttgca agaacaccgt cttgtttcat
     6961 acctgatgcc tgttgtggca tttgcacatc agtttctgca tttcctggta attgaccagt
     7021 cgcacgatag tgagcaaatt cctcaggtgt gacttcgctt ccattaatta agtaacggcg
     7081 attttcagaa ctgtatcctc gcataccacc catcaattgg ttaaataaat catccatgtt
     7141 attaaaatta ttaaagttgt tattcatatt ctttacctct ttttgtttac ttagttatga
     7201 ttactgatat tgactatctt tgaccattgt tttaaaaaat ttagactagc tagatcgcta
     7261 ccctacgtac tatttctggt ttttcttttt caagcaggag tagactatct ttacttctga
     7321 agatttctag atgaaacata gacccacctc tttctatttt actttaaata agtgttctag
     7381 taaggctttc atttacctta cgttcataat atactacatt ggtcagaaaa ggtcaagagt
     7441 tttgacttta actgaccaat attttttaca ctcttcgaaa acctcttcaa accacatcag
     7501 cttcgccttg tctacaacct caaaaccgtg ttttgagctg acttcgtcag ttctatcagc
     7561 catctcaaaa ctgtatatta aaatgaaata agaacaggat aaatcgttca ggacagtcaa
     7621 atcgatttct aacaatattt tagaagcaga tgtgtactat tctagtttca aactactatg
     7681 ttttgagcag cctaaagtta gctttctagt ttgttctttg attttcattg tgtatttaaa
     7741 cacaaaaaca ccctgaaaca tcagggtgcc attcttacat caaatacaaa attgcgaggg
     7801 tcaggagact tgctagaagt cccactcccc aaaagaagaa gtcaaccttc cactcgctcc
     7861 aaggatttcc tttaccagac aatcccccaa gctcaaaatg gtgatgtaca ggcgtcatac
     7921 ggaaaatacg tttaccacct gtcagtttga aataactgac ttgcatcata acagaagttg
     7981 tttcaaaaac atacacaatt ccgataatca agagagtcca ttcttggtgg agagccatag
     8041 agatagctgc cagcatccca cctagggcca aacttcccac atcacccata aagaccttgg
     8101 caggcttatg gttaaagatg aagaaaccga gcaaaccacc aatcatggca agaatcacta
     8161 gaagaatatc catctgacct tgcacatagg caataactcc ataggcagac aaactaatca
     8221 caacggaaat actagctaaa ccgtcaacac cgtctgtcaa gtttactgcg tttgaaaaac
     8281 cgactagcca gaaaagagcg aagaaaatat agaaaaatcc caaatgaact ggataaccaa
     8341 agacagaaag catatcgcca ccgcgctcat agaaaagata gaagataact ccacctagaa
     8401 gctgaagagc taatttttgc ttaggattaa gcccctcatt gattttacga aagaccttga
     8461 gaaagtcatc taaaaatccg accaagccat acaagaccaa gatgaacaaa atcattccca
     8521 cattattgct gaattggcta ctaaataggg cgaaaaagaa agcaaccaaa acagaagtaa
     8581 tcaagaaaac caaacctccc attgtaggag tcccagcttt tgcctgatgc tgtttgacat
     8641 cctcatgcat ctgctggcct gtaatttgcg cctttctata aaattggata aaggccggaa
     8701 ttcctactaa agttagtaaa aatgtcacaa ttccagcact gatggaaata aacatattag
     8761 tctcctaaag ttaatgtaat ttttttaatg tccttgatag ctgtgttagc acgaacatct
     8821 tgcttctgca cagtagagcc cgaaccttga aattcaagtt ctatattgag ccacttagca
     8881 agggtctcag cagtctcctt tgtccaacca tacatatctg gaacttcctc tgctttatca
     8941 gataagataa ggacttgctg attcggggca agattcttcc cttcttcagc agaactgttt
     9001 ttaatcttcg ttcctgttcc cacaacgatg ggttgtacaa gattgcgacg caattcttct
     9061 gctaaatcac caggtgaaat atccttgaca ctaggcatag gataaggact ttgttgactt
     9121 acttgctcca aagctttagc tgttgtttga agattgagag agtctttcat ggctgaagcc
     9181 cgctccaaga taggattggc aaattctccc aactgaatac ctgaataatg ttcaggttgt
     9241 tggaccgtca catacaagat aaaatcagga ttttcagccg gactcatcga tacagccgag
     9301 aaaatatagt cggttaaccc gactagataa ccaccatttt tctcgtcagc aatctgagcc
     9361 gtaccagact tgagggctac attttgccca ggaacagtta cagttggctt gcctgtgctg
     9421 tggttataca tggttccata aaccggatcc gtccctacca aaaccatgtt agtccgagtt
     9481 agactagctg catctttaga aacaggattt cccacaattt ctttttgaga tttccgagca
     9541 gtttgatcat ttggatcata aatggcacta ataaatttag gctccagcat gacaccgtca
     9601 ttagcaatag ctgtaaaggc acgaatcatt tgcgtctggg tcactgaaat cccttgtcca
     9661 aatgagcttt gcgcaatgtt gacaatatta tccgcaggaa gctgaccagc atactcatcc
     9721 gtcaaaccga aacgggtcgg aacaccaaat ttaaaacgat taagataatc aagccaggta
     9781 gcatctccca tcttttgctc aaggagggtc atcccaacgt tacttgagtg tgcaaaacct
     9841 tgagaaaaag tcatcattct gccaccagtc aatccttcat taacgtccca atctcgaatc
     9901 gtggcatctg caatttttaa ctcactacta ttaaagactt ctcctcctgg aaaggtatta
     9961 ttatcaatag cagcagccaa catcatcact ttcatagtgg aacctggctc atagttactt
    10021 tggtaaagga tatcacgcca aacaaagtcc tctgtaatgc cttcttttgt atctgcatca
    10081 aaggtcggtc gttgcgttgt tgccagaatt tcccctgttt tagcactgac caaagtcgct
    10141 gtcatgtact ttccttttac cttctcttga aaagcatcca tctgggtttc cataaaggac
    10201 tggagggggc tggaaatggt tgtataaaca tccttaccat ccatcgttcg ttgggaaact
    10261 tgttctgttc cgggtacaat attacccaga cgatcctttt cataggtaat aatgccgtct
    10321 gtccctgcaa gaatactgtt caaggaactc tccattccag aggttcccag caagctcttg
    10381 cttccatctt cattttcatg gagctgagct aggccgataa aactagaagc aaattgtccg
    10441 tttgggtaac tacgattggg actggttgta aaatcaatcc ccttgacctc tgcagcttcc
    10501 aattcttttt tgatagacat catattggca taggtaatcc catttccctt tgctccaaag
    10561 gaaacttgct tgagattagg ttgcgagagt tgctctctta cataagattc ttccatgtcc
    10621 agatacttat gaaagacctc tgcaaccttg ttaaattgtg ttttttctac gtaaagaatc
    10681 ttacccgttg ctgacttata gttctcatca atgaccgcat agacattata ggaggttgca
    10741 tcctcagcaa tcgggactcc atttcggtca taaatagtcc cacgtttggc aggaactgta
    10801 cgggtggttt gatgaacctt cttagcttcc ttcgctaaat ctgttccaaa gcgagtgcct
    10861 gtcccaataa tgaccgcaaa attgactaaa aaaatggcaa aaacaaagac agataataaa
    10921 ctcagacttt ttccaactct gcgtctgttt tcagccggcg atttccgatt tttggtcgca
    10981 taacggatta ctctttttgt ccacttcata tcttactccg ctattctaat attttcattg
    11041 tttaattgca aatcgtgtga attggcaatt tctttcaaac gttctgcacg taatagttca
    11101 ttgacctctt gcttggcatc gtccaattcg gtcttctttt cctctatctg cgcattgatt
    11161 tttgtcaaat cattctgcac ttgcaagagc ttggtctgca taaaaataat actaatggct
    11221 acaataagag tggttacagc aatggaaaag taaaaagctt tttccacacg cgaaaaccgt
    11281 ttaagttgca tctgtagtat ttgacctgtt ttttccattt tttctgccat ctttttccct
    11341 cttacttgtg aatttttctg gccacgcgca acttggctga gtgcgagcgg ttattggctt
    11401 ctaactcttc cgcacttggc aagattggct tacgggacac caattccatc ttgggcttga
    11461 gatcatctgg gatgaaaggc aagccttttg gaacttcaac tgttgaagct tccttgaaca
    11521 attgcttagt caagcggtct tctaaggaat gaaaggtaat cactgaaatt ctaccatcca
    11581 gagccaacac atccatagcc tgctggatgg actcatctgc cgcccccagt tcatcattga
    11641 cttcaattcg aatagcctgg aaaatctgct tagcaggatg acccttcttc ttgagttcct
    11701 tggcaggttt gaccaacttg ataatctctg ctaactcagt cgttgtctca atcggcttca
    11761 cttcacgcgc ttgctcaatc ttacgcgcaa tctgtttaga gaatttatcc tcaccgtatt
    11821 tgaagaaaat acgaaccaaa tcatgatagt cataatgatt aaccacttca taggctgtca
    11881 gactagcatc ctgattcatc cgcatgtcca gtggcgcatc ctttttataa gaaaaaccac
    11941 gctcacgctg gtccaattga ggactagaca ctcccaagtc ataacaaatt ccatcaattt
    12001 cctgaacacc agcttcgcgc aaacgtgcct gtaaatgacg gaagttatcc ttgataaagg
    12061 tcaccattcc cttctcaatg taaggtgcca agcgtttttg cgcattgtca atggcattct
    12121 gatcctggtc aaaggcatag agatggcctt tttcacttaa tttacttaat aaatactcgc
    12181 tatgtcctgc tccgcccaaa gtcgcatcaa cgtagatacc gtcaggcttt acgtcaagca
    12241 tatcaatcgt ttcgtggagt aagaccgtta catgatgaaa ttcttttgtc atatcttatc
    12301 tattttacca caaatccgac cagcttgcac ttgtcagaca aataggacaa ggagactact
    12361 atttcttcaa aaaatatgtc aaaaatactt gacactttat gtttaaaaga ctataataaa
    12421 gtcaaatata tacgacataa atcagaaagg agaccagatg aatcgtgtga aagaatttcg
    12481 caaggaactg ggcatttccc agctcgagct cgccaaggat atcggtgtct cgagacagac
    12541 tatcaatatg attgaaaatg acaagtacaa tccaaccttg gaactctgtc tcaatctcgc
    12601 ccgcagcctc caaactgacc tcaacagtct cttttgggag gaagattttt aaaaaaggag
    12661 caaactcatg aaaaaagaaa ccttcactga aaaactgatc aaacgcacat acggtatttc
    12721 tggtcccctt gacgaataca aacggcgtga ggccgatagt attgggaacc aagtctttat
    12781 cgtcctcttt tatctgatga ttttcggaaa tcttattcca ctccttctgg cctataaata
    12841 ccctcaagaa gtggctctaa tctatcctcc tctgatttta gtgattgccc tcatcgcttc
    12901 tggctatgtc acctaccaaa tgaaaaaaac aggcatcaca gtcattgaac cagatatgct
    12961 gaatgagaaa gaaagcaagc aactacacta cccaggtctg aaagcaggtt tgttctttgg
    13021 tctatggatg ttttttataa ctcctcttct cagtatactc atagatgaag gtcaggacta
    13081 ttttcattct cttctcacta taagaaatgg tgtatcaagt attctcggtt ctattttctt
    13141 cggagcgagc atacagttcc tcatctcccg tcgcattgca aaaactaaga aaaatcaaga
    13201 tgaggattag gaggaaactc atgaaaaaag aaaagaaaca gttacgttat cctggtctga
    13261 aagcaggttc gatttatgga acagtgatat tttttataat tccactcatt gataccctaa
    13321 caagtgaaaa tccaaatttt atcagttctc ttctaaatac aaaacatatt tttaaaacta
    13381 tactgggagc tttctttttc ggagtgatga tacatattgt cgactcactt cgtattgcaa
    13441 gagctaaaaa agaccaggat taggaggtgc cttatgaaat cactagctag actactgatc
    13501 attcatgttt ttatcagtat tttccttttc ttcgccctta cttcaggagc tatttctcat
    13561 acagttttac tactcctact cctctttctt cctgcgctca ataaaggact tgagaaaata
    13621 caatcaaaac ggatacctgt cctcaacgca gccctcttct ttctcctcat atcctttcca
    13681 caacttttaa ccaaccctgt ccaatggaaa ttttcaatat tcctagtcgt aaccatcatt
    13741 tcaagtttgg cctacttcta taacttttat caagtagtta aagaagtaga tcaaaaacag
    13801 ttgatttagg agcttgctcc cataatttct catttcatag gcaaataatc ccacctagct
    13861 tgttctaggt gggattattt tatttcttaa cagaaattgc aagtcatttt ccttcatttt
    13921 agaaagtcga ctctttccaa ttcacactac aatccaagac ttgttgcctt tcttcttgat
    13981 tgcggtcttc aatctgatca atcaaaatct ttgtagcctg ttgtccttcc tcaaaggagg
    14041 gctgaaccag cgtcgaaaca cttggagaag aaaagcaagt ccactccgta ttgtcaaaac
    14101 caatcaaccc aacttgcggc aagttataat tcaactcttt gataacggta aagactagag
    14161 gtagggccca acagttaggg ataaatacca gagttttttc atcgggatcg atttcttttt
    14221 gtaaaaattc cttaatttgt tccaaattcg tatgcttatc ttcaatggtt agactggcgt
    14281 gacgcatatt agcatctgtt aaagcatcca caaaaccact tgcccgctca atccgagtac
    14341 tcaaacgact cgtatccgct gtaatcaaga gaaaatgctc ataacctttt tcgatacagg
    14401 actgggtcat gtcataaacg gcatcatagt tattggtttt aacccagcta gtccggtgtt
    14461 catagagctg actatcaaaa aagaccattt tcttcttttt ctcatcgatg atacgagaat
    14521 attttcggaa attagaggtc ggctgaataa taaagccgtc tactcccaag agaagcatgc
    14581 tttcaatata ccggtcctca ctctcttggc tgtaattact atttcctatc attacctggt
    14641 agccattctg gctggcgata tcctcaattc ccttaacaat ttggtttgag aaactgttgg
    14701 taatatcacc gatcaaaaca ccgattaatt ttgttcgttt ggagtttaag ctacgcgcaa
    14761 caatgctcgg tttgtaattt gtttcatgaa taactttttc aatcttttca cgtgtctctt
    14821 gggacatttt ttcatatttc ccgtttaggt aaaatgacac ggttgttttc gaggtctgag
    14881 ccatttccgc aatgtctttt atggtcagtt tcttctccaa tctaaaacac ctcctactaa
    14941 tacttattat aacatattca ctattcaaaa tgcctatttc tagcactgca agcgaatcat
    15001 tctctcattt attttatcat atactagaca ttttccacgc atttttgttc cgtcaacgag
    15061 gcacaatttt tcacctacac caatatcctc gcttaataac agaatcagct tgtctgtttc
    15121 gtcatttttc acttcaaaag ccagagcatt ttccacttca cgatcatctg tctggtagac
    15181 agaatgacgg atgatttcaa aactctcatg cgcaatgatg gtataatcca gcatctgatt
    15241 ctcaaagaat tggcgcttgg tgagtttgct acttctttca agctcattgt atttaggaga
    15301 aatgatggta tcttccaaat caaaatcaac ttcactccat agtctcaact gattgatttt
    15361 cccatcttga taggtcacat ccttgtcaag gataaactga gtcaacgcct catgctgacc
    15421 ttgacacctg atgtcatcta ccaagagcca gacatcctct accaacatga ggatttttct
    15481 cttgtgaaga taaggcaaat caggttctgc tgaccaataa gccccctcaa tataatgcac
    15541 tccctccctt tctttatggt gacaaaacag ggagtgagga tagtattcat attcccagga
    15601 tcccgtgatt ctttccggag ctttcccatc tacaatgcag gtcgaatgac tccaagcact
    15661 ctttaagaga taacgttcat atatctcccg ataagaataa cgcccagcat ctatgaaaat
    15721 aggttggcct tgatactgta agcaaaaact attctcgtca ctatggctat gggcacttcc
    15781 tagcggacca tttttgaaaa atagataacg atgttcatcc ttaatgcaga catgtccaga
    15841 gtcttcaaag atcatggact taggctgcca agctctcctt tcaaattcct gcagtcgctt
    15901 gaccttttct cgccccagga acaagaggct aagcaaatca actttaacat ccagaccgtt
    15961 aagaaggtct tccttgttca aaaccacagc agacaggctc aacatttctg tcgtttctgt
    16021 agaatcgcta tcaccaaaag ccaaagtccg tccatctaag cctgtcatca tttgaatata
    16081 ggtcgccatc ttttccagca actcttggta actatcttgc aagtctggaa gcaagagaca
    16141 caaatccagc aaggctttat aaacctctac atgatagaga atcgactgtt caaactggct
    16201 tccatctcct aaaatctgtg tctcaatttg ctgtttcaac tcctctgaag caaaatggta
    16261 agcttcttct aggtccatct tatctgaaaa gaaatgatag atagcaagca tcggaattgt
    16321 ttgtaaaatc ccccagttac taagggtgta cttggcgcga tagtagcttt tcataaagtc
    16381 aatctgcttt tctagactga ccaaaatttt ctctagttct ttctcctcta gcaagtcaaa
    16441 tttcaagaga agcaagagta gtttcaacca agtaaaggaa cgaatacccg tatccaaggt
    16501 tctagtcatc aaggattgag gagaaaattc tctcacccgc tcaatccaat caaatagaaa
    16561 gaacttgcac ttttgaatat agtccttatc tccttctacc acatacccta tcataaactg
    16621 caagagatat tcttgtcgat tgagcatata agcccattct ggatcatctt caaatacttg
    16681 atcccatacc atcggctggt tttgatggat ttttgaacaa ggctccatat cccaaggact
    16741 atcaaacata aaacgattgt ccatcaagcg ttcaagggaa ctcttgactt tctcatagtc
    16801 ttttgaacag tgcgacaaga tataatcacg acattggttt ccatcgattc tttcaaaaaa
    16861 ttgtcttctt tcttctttca ttatctatta ccagaaaaag aactacttaa aaagcagttc
    16921 ttttgtcttt cccattacac tttccttttc tacatagatg accacacctt ttgcaatctg
    16981 caaggagacc aagtcatctt ggatagaaat gatttttcca tgaattccag acaataacaa
    17041 cacttcatca ccagatgtta aagaagctaa atactcttgt cgttgctcca tctgtttgcg
    17101 aagcaacttt tgctgacgaa tagaatgaaa gcttgacagt aaaaggggac tcactgccaa
    17161 gacaatcact attccataaa acaatgttgt atccattaag ctataatctt aagccagctt
    17221 ccgataattc cgatgataac tgttaaaata acgagtttat atgttgtcca tttcttttct
    17281 ttgatcaagt agtaaactaa aagtgtaaat agggctggta gaagagctgg agcaacctta
    17341 tcaagcattc cctgaatact tacgatactt tgtttagcgt ctgctttaac ttcccctgca
    17401 gcaaaggtaa tcggcaccat aatcttaaca gatgtcgctg ccaaaccagc aattacggtt
    17461 acaccgataa tattggcaat acgagaaatc gttgccatct gttcgcttag tttatcaatc
    17521 acagttgttc ctagtttgta tccatacaga ccagttgaca atttaatcgc tgttaaaatc
    17581 gtattcatcg caaggaagaa caagattgga ccgacaacca agccttcttg agcaaacgaa
    17641 gctgcgatgg ttgagaacaa tggagctaaa cagaattgag aaagagaatc cccaatacct
    17701 gccaatggtc ccatcaaggc catcttgata ctacgtgttt cttttgccgg acggccattt
    17761 tccaacatta caaggtgcaa gctggtaata aaaggcagga agtgtgggtt ggtattatag
    17821 aattcacagt tttcttccaa ggcttggtag aaaccttcct gatcctctcc atagtgtttt
    17881 ttcaaagcag gatacatcac attggcatat cccaaccctt gatagttact atagttaaat
    17941 ccattttgac aaaagaatgc ccgcaaagac gttttaagat aatcacgttt tgttaatttg
    18001 ttagatccag tcatcgtgtg cttcctcctc taccacatga tccgctgttt ttggcttgtt
    18061 ataaaattca atcaaagcaa agatagtacc tacaattgca ataccaattg ttgggatgtt
    18121 tagataagct gcacaaacat atcccaacaa gacaaaggga atcaactctt tcttagccat
    18181 cactgacaag atcatcgcaa aaccgatagc tgggagcatt ttaccagcaa ctgtcaaacc
    18241 tgtaagcaat accggtggaa tgtagtctac gagtttcaac aaggtatcca ttgaaagggc
    18301 acccaagcaa cccaaggtaa atccaataaa ggcaaacaac caaattgttg catttagagt
    18361 gaacttgaat ttcttcaaat tatggttttt caagtgcttc atcgctgttt caggcgcacc
    18421 agcacgtaca gtgtaggcga aagtttgtaa gaattgaatc gccacagcaa tcggagtaga
    18481 gagggcaaga gccgcttctg gactgacttt accagcacta gtgatagcca tcaaggtacc
    18541 aaagatacca ggtccgattg ggtttggtgg aacagtacct ccagcaccaa caccgaatcc
    18601 catataagcc aattcaccaa tagctcccat tgcaagagca gtaggcagat cacctagaat
    18661 aattccgaca ccaaatgaca gaacaataca tctattggtg taaattccta acagcattcc
    18721 actaaaacag aaagctgtcc ataatccaat taaaatcgct tgaaatacat taatcgacat
    18781 aacgatctcc tcttttaaat atagtccata atgttgactt gaacagctcc atcatttcct
    18841 gttggagttg ttttcgtatt aaatgttact tgatgagttt ggctcaattc cttgagggcc
    18901 accttgtcct cttcacctag gaagatggag cgtgtcactt gctctttacc agtagcattg
    18961 tgaatgttcc caatattgat ttctttgata gggacaccac cttctaccaa ggttaaagca
    19021 tccttcacat tctttacaac gataaagatc gtttgagcag gattagcctt gtgaatgata
    19081 tcaatcacct tttgcaaagg gaagaaacgc atggcaactg agtctggcac aactgttttc
    19141 atcagagttt gttgcatctt gtccgtgctt acttcgtcat tggcaacaat gaccgtatta
    19201 caacctaggt attttaccca aagttgtcct tgtccatgaa tcaaccgttc atcgatacgg
    19261 gtcataataa tatttggcat tgtcatattt ctcctcctac caatataggt tccagtcttt
    19321 gtagaaacgg ataagggctt ctaggtaata gtagtcaccc cagatattgc cttcatccac
    19381 tcctttacct gaatgccatg agtacacacc gtggaggaga cttgtcccac caggggtaaa
    19441 ttgatcattt gcataatgtt cgatcaagga acgaagcatg gcatgcatag catgtttata
    19501 aatatctttg tcagcatcca cctctgggag atgttttagc atttcatgaa tcccacagac
    19561 ggcgatagct gttgctgaag aatctcgtga ttgatcacta ccatcattaa aaatcaaatc
    19621 ccaataggac acatgatctt ttggcagacg attcaagaaa taattggtca cacccttaaa
    19681 caagtcaaag caggactcgt cttttaagtg acgataagtc aaaggaatac catagactcc
    19741 ccatgattga ccacgtgccc agcatgaatc atcactatac ccttgtctcg ttacaccttt
    19801 aaagggttga cctgtctcag gatcaaaata gaaggtgtgg aaggacgaag cgtcatcacg
    19861 gattacatta ttagctgaag catagaaatg actttctgca atatcgtagt atttttgatc
    19921 gcctgtttct tgataagcaa agaataagag ttggatattg agcaagcagt cgataatcaa
    19981 acggtaatgc tctttcttgc ccaagtctcc ccaagcttga ataaaaccac ctttttcttg
    20041 atagcgttca atcaacttat ctgcagcttt caaggttgct tctctagcct ctccatctcc
    20101 atttatctta tattcagcca tacaagacgg tgtgtacaag aagccgagat catggtgatc
    20161 caattctact ctcttattga cacgatccag gaaagaaaga acatttttat gagcgatgtt
    20221 tttaaatgca tcctgttgac tgtattcata agccaaccac agttctcctg tccagaaacc
    20281 attggtccat tccgtgttat ccatgattgg atagacatta tcaaaggtag ctggtgtcgg
    20341 gaaatcttcc ttgaaatagt caaggttgag ttctaactgc cgaataacct tatcgattgc
    20401 ctggccgact tcttctttcg tcagaagtgg tacttctaag aagcgctcag gcgattttat
    20461 tttttcaatc gtaacctttt ttatcattct tttacccttt aaatacccga ttcgaaatct
    20521 tcttcctctt cctctgcatc cgttgaaaac aattctttac catttacgac tccgccctgg
    20581 gccgccacta ctgatttatt aacaacttca tcaaagctat gagccattct agcaaagact
    20641 gcttccatta acatggctaa gttcaaaccc gagagaacat tcattgtttt ggctggattt
    20701 tctcccatta tggtagaaga aaccttgaat ggcgatcctc ccaagagatc acttaggatt
    20761 aaaacttctt cttcatttga aattgcaagt aagatttttt gcttgagttc atctgctgac
    20821 attccttcca caaagtcaat cgcctccaca ttttcttgat tgcctgcaat caattgtaaa
    20881 gaactataaa tccctgtagc aaaatgtcca tgccctacaa gtacaatttt catcttttct
    20941 cctttcttgt ttaggtttac cggtttactt
//
LOCUS       DAVGJJ010000030        20829 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000030 DAVGJJ010000000
VERSION     DAVGJJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.030"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            6..689
                     /locus_tag="VVE35_001006"
     CDS             6..689
                     /locus_tag="VVE35_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3061218.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            689..1423
                     /locus_tag="VVE35_001007"
     CDS             689..1423
                     /locus_tag="VVE35_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3061219.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVIENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1577..2434
                     /locus_tag="VVE35_001008"
     CDS             1577..2434
                     /locus_tag="VVE35_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU3061220.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2576..3448
                     /locus_tag="VVE35_001009"
     CDS             2576..3448
                     /locus_tag="VVE35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU3061221.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3730..4413
                     /gene="rpiA"
                     /locus_tag="VVE35_001010"
     CDS             3730..4413
                     /gene="rpiA"
                     /locus_tag="VVE35_001010"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU3061222.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4427..5638
                     /locus_tag="VVE35_001011"
     CDS             4427..5638
                     /locus_tag="VVE35_001011"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU3061223.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5640..6182
                     /locus_tag="VVE35_001012"
     CDS             5640..6182
                     /locus_tag="VVE35_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU3061224.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            6198..7007
                     /locus_tag="VVE35_001013"
     CDS             6198..7007
                     /locus_tag="VVE35_001013"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU3061225.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7483..8463
                     /locus_tag="VVE35_001014"
     CDS             7483..8463
                     /locus_tag="VVE35_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU3061226.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNNRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGKQVTQSSTVFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            8488..9000
                     /locus_tag="VVE35_001015"
     CDS             8488..9000
                     /locus_tag="VVE35_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU3061227.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            9658..10368
                     /gene="deoD"
                     /locus_tag="VVE35_001016"
     CDS             9658..10368
                     /gene="deoD"
                     /locus_tag="VVE35_001016"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU3061228.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10391..10916)
                     /locus_tag="VVE35_001017"
                     /pseudo
     CDS             complement(10391..10916)
                     /locus_tag="VVE35_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
     gene            complement(10969..11205)
                     /locus_tag="VVE35_001018"
     CDS             complement(10969..11205)
                     /locus_tag="VVE35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU3061229.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(11273..12193)
                     /locus_tag="VVE35_001019"
     CDS             complement(11273..12193)
                     /locus_tag="VVE35_001019"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU3061230.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            12383..12786
                     /locus_tag="VVE35_001020"
                     /pseudo
     CDS             12383..12786
                     /locus_tag="VVE35_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13002..13583
                     /locus_tag="VVE35_001021"
     CDS             13002..13583
                     /locus_tag="VVE35_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3061231.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            13580..14857
                     /locus_tag="VVE35_001022"
     CDS             13580..14857
                     /locus_tag="VVE35_001022"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU3061232.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            14875..15537
                     /gene="deoC"
                     /locus_tag="VVE35_001023"
     CDS             14875..15537
                     /gene="deoC"
                     /locus_tag="VVE35_001023"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU3061233.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            15524..15913
                     /locus_tag="VVE35_001024"
     CDS             15524..15913
                     /locus_tag="VVE35_001024"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU3061234.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            16003..17055
                     /locus_tag="VVE35_001025"
     CDS             16003..17055
                     /locus_tag="VVE35_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU3061235.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDIANKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            17199..18734
                     /locus_tag="VVE35_001026"
     CDS             17199..18734
                     /locus_tag="VVE35_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061236.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            18727..19785
                     /locus_tag="VVE35_001027"
     CDS             18727..19785
                     /locus_tag="VVE35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061237.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            19788..20744
                     /locus_tag="VVE35_001028"
     CDS             19788..20744
                     /locus_tag="VVE35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061238.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6097 a         3708 c         4812 g         6212 t
ORIGIN      
        1 tgactatgaa tttttctttt ttacctaagt atttacctta ttttaactat ggggctgttg
       61 tgacgattct tatttctatc tgtgttatct ttttgggaac tattttgggt gttgtcttgg
      121 cttttgggca acgttcaaag tttaaaccgc ttgtttggtt ggccaacttg tacgtttgga
      181 ttttccgtgg gacaccgatg atggttcaaa ttatgattgc ctttgctctt atgcatatca
      241 atgctccgac tattcagatt ggaattttag gtgttgattt ttcgcgtctg attccaggga
      301 ttttgattat ctctatgaat agtggtgctt atgtttcgga gactgttcgt gccggaatca
      361 atgcggttcc aaaaggtcag ctagaggcgg cttattcgct agggattcgt cctaaaaatg
      421 cgatgcgtta tgtgattttg ccacaagcag tcaaaaatat cttgccagca ttggggaacg
      481 aatttatcac cattatcaag gacagctccc tcttatcagc tattggggtc atggagttgt
      541 ggaatggggc tacaacagtt tctacaacaa cctatctacc tttaacacca cttttatttg
      601 cagcatttta ctacttgatt atgacctcta ttctgacagt agccttgaaa gcttttgaaa
      661 aacatatggg acaaggagat aagaaataat gacagaaacc ttgataaaaa ttgaaaattt
      721 acataaatct tttggaaaga atgaagtatt gaagggcatc aacctcgaga ttaaaagagg
      781 agaagttgtc gttatcatcg gtccttcagg gagtgggaaa tctaccttgc ttcgctctat
      841 gaatttgttg gaggaagcaa ccaaggggaa ggttatcttt gagggagtcg atattacgga
      901 caagaagaat gacctgtttg ccatgcgtga gaagatgggc atggttttcc aacaatttaa
      961 cctctttcct aatatgactg tgattgaaaa tattaccttg tcacctatca agaccaaagg
     1021 tgaaagcagg gaagttgctg agaagagagc ccaagagctt ttggaaaaag ttggtttgcc
     1081 agataaggca gacgcttatc cacagagtct gtcgggtggg caacaacaac ggattgccat
     1141 cgcgcgtggg ttggctatgg aaccagatgt tttgctcttt gacgaaccaa cttcagcctt
     1201 ggatcctgaa atggtaggtg aggtattggc tgttatgcaa gaccttgcca agtcagggat
     1261 gactatggtt atcgtaacac atgagatggg atttgcccgt gaggtggcag atcgtgttat
     1321 ctttatggca gacggtgtgg ttgttgaaga tgggacacct gagcagattt ttgaacaaac
     1381 ccaagaacaa cggactaaag atttcttgag taaggtttta taatctattt taaaatttca
     1441 agacaagatt agtgaaaagt tcaaccagag ctttttctta tagtttgaaa ctataggata
     1501 acctaggaaa gaagtgttag agggcactag cagtttttgg tataatggaa gatatttgaa
     1561 agaaaagaga agtgatatga cacagattat tgatgggaaa gctttagcgg ccaaattgca
     1621 ggggcagttg gctgaaaaga ctgcaaaatt aaaggaggaa acaggtctag tgcctggttt
     1681 ggtagtgatt ttggttgggg ataatccagc cagccaagtc tacgttcgca acaaggagag
     1741 gtcagctctt gcggctggtt cccgtagcga agtagtgcga gttccagaga ccattactca
     1801 agaggaattg ttagacctga ttgctaaata taatcaggat ccagcttggc atgggatttt
     1861 ggtccagttg ccattaccaa aacatatcga tgaagaggcg gttttattag ccattgatcc
     1921 agaaaaggat gtggatggtt tccatcccct aaacatggga cgtctttggt ctggacatcc
     1981 agtcatgatt ccctcgacac ctgcaggaat tatggaaatg tttcatgaat atgggattga
     2041 cttggaaggt aaaaatgcgg tcgtcatcgg tcgttcaaat atcgttggaa aacctatggc
     2101 tcagcttctt ttggctaaaa atgcgacagt aaccttgacc cactcacgaa ctcataatct
     2161 tgccaaggtg gctgctaaag cagatattct tgtagtcgca atcggccgcg ccaagtttgt
     2221 gactgctgac tttgtcaaac caggtgcggt tgtcattgac gttgggatga atcgagatga
     2281 aaatgggaag ctctgtggag atgttgatta tgaggcagtt gcaccacttg ctagccatat
     2341 cacgccagtc cctggaggtg tcggtcctat gaccattacc atgctgatgg agcaaactta
     2401 tcaagcagca cttcggacat tggatagaaa ataagagaaa cattcgatta atagagtgta
     2461 ttttctatag ctatatctaa aatggcagaa atgaatatta aattttagat ataagattac
     2521 aaaaggaggt ctgcgtctcc tttttattgt ataataaaag tgagaggaaa aaaggatgaa
     2581 agtgattaat caaaccttat tagaaaaagt tattattgaa cgttctcgtt caagtcataa
     2641 aggagattat ggtcgtctgc tgctgcttgg tggtacttat ccttatggag gtgccatcat
     2701 catggctgct ttagcagctg taaaaagcgg tgcaggattg gtgaccgttg gaacggacag
     2761 ggaaaatatc ccggctctgc acagccattt acctgagtct atggcctttt ctcttcaaga
     2821 ccagcaattg ttaaaagagc aattggagaa ggcagaagtt gtcttgctgg ggcctggttt
     2881 acgagacgat gcttctggag aaaatctagt aaaacaggtc tttgttaatt taagccaaaa
     2941 tcagattttg attgtagacg gaggtgcctt gaccattctt gctaggacaa gtttgtcatt
     3001 tccctcaagc cagcttatcc taactcctca ccaaaaagaa tgggaaaaat tatctggaat
     3061 tacgattgaa aagcaaaagg aagatgcaac ggctagtgtt ctgacttcct tccctcaagg
     3121 aacaattttg gtggagaaag gcccagctac tcgtatttgg gaagttggcc aatctgatta
     3181 ttaccagtta caggttggtg gtccttatca ggcgactggg ggaatgggag atactctggc
     3241 tgggatgatc gcaggatttg taggccaatt ccgacaggcc agtctctacg agcgtgtggc
     3301 agtagcgacc catcttcatt cagccatagc ccaagaacta tctcaagaaa attatgtggt
     3361 cttaccgacg gaaatcagca ggtatcttcc caaaataatg aaaattattt gtcaacaaga
     3421 gagggtaagc aaagataaac ttgtttaaaa tagacagata aatctacttc cttctcttaa
     3481 tttatgattc ttgaaatgcg cctgctttaa ggctaaacta gacgtatcaa agttaagagt
     3541 ttgtttttgt aactggaaag agatgaacaa atcaaataca taaaagataa actcattttt
     3601 attcgtttgg taaaaataaa atgcatattt ttaaagaaag attgatgaaa aatgtagatt
     3661 tgatgttttt acttgaataa aaagcggttt catagtaaaa taagaattga gaatgatgga
     3721 ggtaaatcgg tggaaaatct gaagaaaatg gcaggtatca aggctgctga gttcgtgagc
     3781 gatggaatgg tcgttggact tggaacaggc tcgactgcct attattttgt cgaagaaatc
     3841 ggtcgtcgta tcaaggaaga aggattgcag attacagctg taacaacttc tagtgtgacc
     3901 actaaacagg cagaaggcct taatatcccg ctcaagtcta ttgaccaagt agactttgtc
     3961 gatgtgacgg ttgatggcgc agatgaagtg gatagtcagt ttaatggaat caaaggcggt
     4021 ggtggtgccc ttctcatgga aaaggtggtc gcaacaccat caaaagaata catttgggtg
     4081 gtggatgaaa gcaagctggt cgaaaaacta ggtgctttta aattgccagt agaagtggtt
     4141 cagtatggtg cagagcaggt ctttcgtcat tttgaacgag ctggctacaa accaagtttc
     4201 cgtgaaaaag acggccaacg ttttgtgacg gatatgcaga attttatcat tgacctcgcc
     4261 ttggatgtca ttgaaaatcc aattgctttt ggacaagaat tggaccatgt cgttggtgtt
     4321 gtggagcatg gtttattcaa ccaaatggtg gataaggtaa tcgttgctgg acgagatgga
     4381 gttcagattt caacttcaaa aaaaggaaaa tagaaggggg cataagatgt ctaaatttaa
     4441 tcgtattcat ttggtggtac tggattctgt aggaatcggt gcagcaccag atgctaataa
     4501 ctttgtcaat gcaggggttc cagatggagc ttctgacaca ctgggacaca tttcaaaaac
     4561 agttggtttg aatgtcccaa acatggctaa aataggtctt ggaaatattc ctcgtgaaac
     4621 tcctcttaag actgtagcag ctgaaatcaa tccaactgga tatgcaacaa aattagagga
     4681 agtatctctt ggtaaggata ctatgactgg acactgggaa atcatgggac tcaacattac
     4741 tgagcctttc gatactttct ggaacggatt cccagaagaa atcctgacaa aaattgaaga
     4801 attctcagga cgcaaggtta ttcgtgaagc caacaaacct tattcaggaa cggctgttat
     4861 cgatgatttt ggaccacgtc agatggaaac tggagagttg attatctata cttcagctga
     4921 ccctgttttg cagattgctg cccacgaaga cattattcct ttggatgaat tgtaccgtat
     4981 ctgtgaatac gctcgttcga ttacccttga gcgtcctgcc cttcttggtc gcatcattgc
     5041 tcgcccttat gtaggtgaac caggtaactt cactcgtacg gcaaaccgtc gtgacttggc
     5101 tgtatctcca ttttccccaa ctgttttgga taaattgaat gaggctgata tcgatactta
     5161 tgctgtgggt aaaatcaacg atatctttaa cggtgctggt atcaaccatg acatgggtca
     5221 caacaagtca aatagtcatg gaattgatac actattgaag actatgggac ttgctgagtt
     5281 tgaaaaagga ttctcattca caaacctagt tgactttgat gccctttacg gccatcgtcg
     5341 taatgctcac ggttaccgtg attgcttgca tgagtttgat gaacgcttac ctgaaattat
     5401 cgcagctatg agagagaatg accttctctt gattactgcg gaccatggaa atgatccaac
     5461 gtatgcagga acggatcaca ctcgggaata tattccattg ttggcctata gccctgcctt
     5521 taaaggaaat ggtctcattc cagtaggaca ttttgcagat atttcagcga ctgttgccga
     5581 taactttggt gtggaaactg ctatgattgg ggaaagtttc ttagataaat tggtataaga
     5641 tgacgcgcta tgctttgctg gtgagaggta tcaatgttgg tagtaagaat aaggtcgtca
     5701 tggcggagct tcatcaagaa ttgacaaact tgggactgga aaaggttgag agctacatca
     5761 acagtggcaa tattttcttt gcttcgatag attccaaagc ccaattggtt gaaaagctag
     5821 agactttctt tgcagtccat tatccattta ttcagagctt ttctttactg agtctagagg
     5881 actttgaggc ggaacttgaa aatctaccag cttggtggag cagagacttg gcacgaaaag
     5941 attttctctt ttacactgag ggtttggatg tggaccaagt catcgcgaca gttgaaagtt
     6001 tagagctgaa agatgaagtg ctttattttg gaaaacttgg gattttctgg gggaaatttt
     6061 ctgaagaatc ctattctaag actgcctatc agaagtactt gctgaaggtg cctttctacc
     6121 gccacattac tattcgtaat gctaaaacct ttgacaaaat tggtcaaatg ctaaaaaaat
     6181 aataaaggag acacacaatg acatttttaa acaaaatcca tgaaactgct actttcctga
     6241 aagaaaaggg aattgcagcc cctgagttcg gtctaatcct tgggtcagga cttggagaat
     6301 tggcagaaga aatcgaaaat ccagttgtag tagactatgc tgagattcca aactggggcc
     6361 gttcaacagt agtcggtcat gctggtaaat tggtatatgg tgaactggca ggtcgcaagg
     6421 tcttggctct tcaagggcgt ttccatttct atgaagggaa tcctctggaa gtggtgactt
     6481 tcccagttcg tgtgatgaaa gttcttggat gtgaaggtgt tattgtaacc aatgcagctg
     6541 gcggtatcag atttggtcct ggtaccttga tggctatctc agaccatatc aacatgacgg
     6601 ggcaaaatcc attgatgggt gaaaacttgg atgactttgg cccacgtttc ccagatatgt
     6661 ctagggccta cacaccagaa taccgtgcca ctgcccatga agtggctaaa aaacttaata
     6721 tcaagattga tgaaggtgtc tatatcggag ttactggtcc gacttatgaa acaccagcag
     6781 aaattcgttc ctataagaca ctgggagcag atgcagttgg tatgtctacg gttcctgaag
     6841 ttatcgtggc agcccactct ggcttgaaag ttctgggaat ttcatgtatc actaactttg
     6901 cggctggttt ccaagaagaa ctcaatcacg aagaagttgt agaagtgact gaacgtgtta
     6961 aaggtgattt caaaggcttg cttaaagcga ttcttgctga attgtaagaa aaaagattta
     7021 aaagggggag tgcctctgtc ttttcaggat tgactgccta tccgtattaa agaagaaaca
     7081 gaggaatact atgagcttct tcctgctctt ataactgaaa gaagcggaag aataggtgtg
     7141 tctgatctga tagccagcat tgtgaaagac aagattctag gatactagca ttagcttcct
     7201 agccaagcag actagtatga taaggagaga tgagaatgaa ttgactttct gaatttctca
     7261 gtcttatcat atatagcaca atgagatttc gcttgagtct gcttgtaaat aaacgaaaag
     7321 aaagataaga aataatgaaa attggtcaac gaattatgcg ctttggcata aaaaattaag
     7381 tatcggagtt gtatctgttg tagtcggctt tgatttctag ctccagctgg aatttcagcc
     7441 aatgaagtaa agcaagatgt aacatctgaa gtggtaatag gtgtgctaga ttctaaggag
     7501 gaattgaaag agtcagaaaa tgatgctcca aaactagaaa ctcctcttag agaggagcca
     7561 agactagctc ctcaaacgct tccggaagca agtgaagttc ttgaaaacaa aagggaagag
     7621 tcaaaagtag agataacaga gccagctcaa gcggatgata tccgcaaggt tgttggggaa
     7681 ttagccaagg atataagtat tactaagttg tatatgacag gtcattctct tggatgttac
     7741 ctagctcaga ttgcagcggt tgaagcttac caaaaatatc ctgattttta taaccatgta
     7801 ttgaggaaag tgacaacttt cagtgctcct aaagtgatta cttccagaac tgtttggaat
     7861 gctaagaatg gtttctggga tgttggtttg gaaagtcgta aattagctgt tagtggaaaa
     7921 attaagcatt atgtggttga taatgacaat gttgtgactc ccttgattca taataatcgt
     7981 gatattgtta catttacagg taattcacgc tttaaacacc gttctcgtgg ctattttgaa
     8041 agtccaatga atgatattcc taactttaat attggtaaac aagctacctt ggataaacat
     8101 ggttatcgtg atccgaaatt ggataaagtg cgattcttta agaaacaggc tctgcctcga
     8161 tcttctagtc aaccaagcgc tgaaccaatg gaaaatattg ccttaggaaa acaggttact
     8221 caaagttcga cagttttcgg aggagatgct agaagagctg tggatggcaa agtcgatggt
     8281 aactatggtc acaattctgt cactcataca aacttccaat ctaagccttg gtggcaagta
     8341 gatttggcta aagaagaaac cattcgccaa atcaatattt acaaccgaac agacactgcc
     8401 caggatagat tggcaaactt tgatgtcatt cttttagaca gttctggtaa agaaattgag
     8461 taaaaacgta taacatctcc taaagatgtg tcagcacaaa ttacgattaa ccataaaaaa
     8521 gtgcgctatg ttcggattga gctagaaggc tataatgccc tcagtcttgc agaagttgaa
     8581 gttttccgct ttatagctac gaatgctgaa acggcgacac aagtttctaa gccagttcaa
     8641 ccaatcagtc agactcctgt gaaagataaa acattgacaa ttcaacacag tggagcttac
     8701 attgcccgct actccataac ttgggaagaa gttccggtag ataaagatgg aaaccaagtt
     8761 gttcgtagtc attcttggga aggaaacggt tgcaaccaga ctgcaggttt tgtcctcaac
     8821 ctcccaatca aagaaaatat gagaaatctg cgagttaaga ttgagaaaaa gacgggccta
     8881 ctatggaata gatggcaaac aatctatgaa aacagaccaa ttttagctca accccaccgt
     8941 aaaattaccc attggggtac gacattgaat tccaaggtga gtgacgatga tgtcttgtaa
     9001 tctgatggta caatgacagt tagtttgtct agtttataag aaagtactac ctgagcttga
     9061 atagaactca ggtagctctc tgtgaaagaa caaaattaat actcaatgaa aatcaaagag
     9121 caaactagga aactagccgc aggttgctca aagcactgct ttgaggttgt agataagact
     9181 gacgaagtca gtcacatata taatccaagg cgacgttgac gtggtttgaa gagattttcg
     9241 aagagtataa acagaaaggt agagcgcgtg ttctaatttg aacacgagta gaaaactttt
     9301 ctaaaaacaa aaacgaaagg atgggtaaac tgtattcgct gaactgaata cgggcgactc
     9361 tcctctaaat caaaattaag aaagaaagga attgaaccca ccctaaaagc agtgggaaaa
     9421 agatagttgg tctagcgagc atcgctcact gcgcccaact cctattttcc cttcgctttt
     9481 tgacgggttt ggtatctttc tcaatataaa atataaaata aagaaaggta gagcgtgtgt
     9541 tttgatttga acacgagcgg aaaactcgga aaatagataa tctgactgaa aaatcaggat
     9601 ttctcgtcgg cttcctaatt ttcagtcgtt ttcttctcgc tctttgtatc ataaattatg
     9661 tctatccata ttgctgctca gcagggtgaa attgctgata aaattcttct tcctggggat
     9721 cctcttcgtg ctaagtttat tgcggagaat ttccttggtg atgctgtttg ttttaacgaa
     9781 gtgcgtaaca tgtttggtta cactggtact tacaagggtc accgtgtatc tgtcatggga
     9841 actgggatgg gaatgccatc tatttcgatt tatgcgcgtg agttaatcgt agactacggt
     9901 gtgaagaaat tgattcgtgt gggaactgca ggttctttga atgaagaggt tcatgttcgt
     9961 gaattagttt tggcgcaggc ggctgcaacc aactcaaaca tcgttcgtaa tgactggcca
    10021 cagtacgatt ttccacaaat tgctagcttt gatttgcttg ataaagccta ccatatcgcc
    10081 aaagaacttg gtatgactac tcacgttggg aacgttttgt catctgatgt cttttactca
    10141 aattactttg aaaagaatat cgagcttggt aaatggggag tcaaggctgt ggaaatggaa
    10201 gcagcagctc tttactatct tgctgcccaa taccatgttg atgcgctagc tatcatgacc
    10261 atctctgata gcttggtcaa tccagacgaa gacacaactg cagaagaacg tcaaaatacc
    10321 ttcactgata tgatgaaggt tggtttggaa accttgattg cagaataatt atagccaaaa
    10381 aggaagccct ttatgaattg taggacttcc ttttcttatc cagaaattga tctagctctc
    10441 tctgatttcg aagaatagtg actttatgtg aatattcttg gcaaagtttt tggtaatttt
    10501 ctttttgagt tttgctacgc ccatcccaaa gaatccatct gataaactcc cactcaaagc
    10561 gttcagggca atctaccgcc atactttctc tgatcttttc cacggtattt aagataacgc
    10621 ttaaaggctc taaagagaca ggtcaatggc gaaaaattga gaaagatgat ttggtcagct
    10681 tcttgcattc gttcttggta gtagcaccaa gaataattac catcgatgac ccaagcttta
    10741 tgcttggtga gaaagttttt tatctcggtt aacatccatt cgcagtcact gtcttgccaa
    10801 ccaggttgaa attggagtgt gtccatgtgc agttttggaa tggagtagta gttagataac
    10861 ttttctgcta tagttgactt accagaacca gaatatccga taattgcgat tttcattttc
    10921 taccttttcc tatttggaga caaaaaaaca gcctctatgg actgtttctt atttaacaag
    10981 tttagctgaa agacgagctt tatcgcggct tgctttgttt ttgtgaatca aacctttagt
    11041 ttctgcttta tcgatagctg agctagcagc acggaaaagt tcttcagatg ggtttgcttc
    11101 gaaagctttt atagcagtac gcatagctga tttttgagct gagttctttt cgttttgttt
    11161 aacgttcaat tcagcgcgtt tgatagctga tttaatgttt gccaatgttc ttacctccat
    11221 atttactaac tataccatta tatatgaaaa cttacgtttt gacaagggaa aattactttt
    11281 ttaagtaaat ttcatcgatt tcatggttct tgcttttatg aagaatcact tctgcacgat
    11341 ttctggttgg ttcaatataa ttttgcagat ttgtgagatt gatactgatc cagacctgat
    11401 gggcaaagga ctccacttcc ccaatcggca tctgagtaaa acgataatag tagctatcag
    11461 ggtcgttttg ggctaggctc agcattttca agaaacggtc cagataccaa ctttcaatat
    11521 catccactcc agcatctaca tagatggaaa agtcaaagaa gtcagtgata tagagacgat
    11581 cgttttgtgg attttgaaag acattaattc cctcaacaat tacaaaatca gcagctttga
    11641 cactttgttt cttttcgggt acgatgtcgt aaacttcatg agaatagaca ggaatatcta
    11701 catcttgtcc atttttgatg cggtccaaga agttgagaag agcttccata tcatagcttt
    11761 caggaaatcc tttacgattt aaaatcccct gctcaatcaa ggtttgattg ggatagagaa
    11821 aaccatcagt tgtaaccaac tcaaccgtag catctgtaaa cgtacgggac agtaggattt
    11881 gaagtaggcg actggttgtg gattttccaa cggcaacact cccagaaacc ccaataataa
    11941 aaggttgaga tttactttca cgttggagga aaattccttt tgaaaaggct aaatcttcct
    12001 tagttcgctt gtaaatctga atcagatgag ccaaggggag atagatatct gtaacgtctt
    12061 ggagactgat ttggtcatta aaactcttga tagattccaa ttcttcttct gtcaaaggag
    12121 gtgttgtctt tcgatgtaaa gattgccaag tctggcggct gattttttca aaatgtaaaa
    12181 attcgttggt catttgtttt cccctagata ttttgtctat cataccataa aaagctaaaa
    12241 aaataaagcg gtttcttgaa tgttgtaagt caatgatata taatataagt gatcaattta
    12301 ttgatgaaat aggagataga ttctactgat ttataagctt ataaaggggg ctttttagtt
    12361 cacaacttat aggaggtgta ctatgaaaat cttaaaacgt tacatattgg aactctgttt
    12421 tattttaagt tttgctttac cttttataaa aggaaccaat gcagataatg gtagatgctt
    12481 tgtggaaacc tattacggtt ttactttttt gatggaacat gctattgtaa cagctgtctt
    12541 tatttgttcg ttcttaattg ctttcttact aaaaaacgat ggacgaaatg gattgctgcg
    12601 ggtagttatt gttttttagt cctacggatt gcgacagagg gttatttctt ccacatgtca
    12661 ctagaagatt tgatacgact ttggacaagt ttggaaatcc tgacacaaac gtatcagttg
    12721 ggcttttatc taaacatttt gttaggaatt ttgttgataa taaagtattt taaggttaag
    12781 caatagaaat aataatctgc ctgattatag atgataaact ttttgttttt atctggactg
    12841 tgatatttat agttctaaat ggtttattat ttataagtgt tgaaagagat tgaagaaaat
    12901 ggcttcgtga cttgctttct tcggtctttt tcgttttatt atcaatttgt aaacgattta
    12961 caatttgagt caaattatgg taaaatagtg tcatgagtaa aatgtactat gcagaaaatc
    13021 ctgacgctgc tcacgacatt catgagttga gagtggactt gttgggagaa aaaatgactt
    13081 ttttgacaga tgcgggtgtt tttagcaaga aaatggttga ctttggaagt caactcttgc
    13141 tcaagtgtct ggatgtcaac caaggagaga cagtcctaga tgtaggctgt ggttatggtc
    13201 cattaggttt gtccttggtc aaggtttacg gagttcaggc gaccatggtt gatattaaca
    13261 ctcgtgccct ggatttagct cagagaaatg ctgaaaaaaa taatgcaaaa gcgacgatat
    13321 ttcaatctaa tatttatgca agagttcaag ggcgatttga ccatgtcatt tccaatcctc
    13381 ctatccgtgc gggcaagcaa gtcgttcatg aaatcatcga gaaaagtaaa gatttcttgg
    13441 aaattggtgg agatttgacc atcgtcatcc agaaaaaaca aggagctcca agtgccaagt
    13501 ctaagatgga agatgtgttt ggcaattgtg aaatccttaa aaaagacaag ggatactata
    13561 tccttagaag tgtgaaagaa tgagagcagt tgatttaatc caaaaaaaac gagacggtca
    13621 agaactgact tcaagtgaaa ttgaatggct gatagaaggc tatgtatcag gaactgttcc
    13681 tgattatcag atgtctgcct ttgctatggc tgtttatttt aaaggaatga cgactcgtga
    13741 aatttctgat ttaacgatga atatggttaa gactgggcaa gagtttgacc tgtcagccat
    13801 tgatggtgtg aaagttgaca agcattctac tggtggtgtc ggcgataagg taaccttgat
    13861 tttagctcct cttgttgcta gcttcggtgt gcctgtagca aaaatgagtg gtcgtggtct
    13921 tggccataca ggcggaacaa ttgataaatt ggagtccatt aagggctatc aagtagaacg
    13981 tagtcaagag gattttattc gtcaggttca ggatattgga gtatctgtca ttgggcaatc
    14041 agaccagctg gtcaaagcag ataagcttct ctatgccctt cgtgatgtga ccgcaactgt
    14101 cgacacgatt cctttgattg cgagttcggt gatgagcaag aaaattgcgg caggggcgga
    14161 tgctattttg ctagacgtga ctgtcggtga gggtgccttc atgaagacgg ttgatgaggc
    14221 gcgtgagttg gctcaaacta tggtagatct tggtaaggtt gttggacgga agacagtagc
    14281 agtgattacg gatatgagcc agcctttggg acgagcgatt ggaaatcgtc tggaaatcct
    14341 tgaagcattg gagattttgc aaggtcaagg acgtcaggat attacccact ttatttgtga
    14401 attggctcaa attatgcttg gtttggcaaa tgttaacaaa actgttgaag aagttcgcca
    14461 acatcttgaa aatggtcaag cactggctaa gtttgaggaa atggtgcaag cccaaggtgg
    14521 agacttggaa gacctctatc gtcctgtaaa tgtagctcat gtggtggaaa tccctgctca
    14581 ggaaacgggt gtcatttcag ctcttccagc tatggacttt ggtctttatg ccatgaggct
    14641 gggtgctggt cgtgcagtta agtctgatgt cttggactat gaaacaggaa ttgtttttga
    14701 aaagaagatt ggagactccg ttcaaaaggg tgaaattgtt gcaaaagtat acacaaatga
    14761 aaaaattcca cctcaactag ttacagattt ccaaaaatgt gttaaaataa gtgatgaagt
    14821 gaaaaaaatc cgtgaaattg tagaaatcat ctcctaaatg gagggaaaac cgaaatgaaa
    14881 ttaaataaat atatagatca tacgctttta aaacaagatg caaagaaaaa acaaattgat
    14941 agtttgttgt ctgaggctag agagtatgac tttgccagtg tttgcgttaa tccgacctgg
    15001 gttgaacatg ctaaaaaagg acttgaaggc acagatgtta aggtttgcac agtagtaggt
    15061 ttccctttgg gagcaacaac ttcagccgtg aaagcatttg agacaaaaga agctatccaa
    15121 aatggtgcag atgagattga tatggtgatc aatgttgggg ctctcaagtc aggtgatttg
    15181 gccttggttg agtcagatat tcgcgcagta gtggaagcaa gtggtgataa gttagtgaaa
    15241 gtcattattg aagcttgcct tctgacagac caagaaaaag ttgttgtttg ccaattggcc
    15301 caaaaagctg gggctgactt tgtcaaaaca tctactggct tttcaactgg tggtgctacg
    15361 atagcagatg ttacattaat gcgtgaaaca gttggatctg atatgggtgt caaggccgcc
    15421 ggtggagctc gttcttatgc agatgctctt gcctttgtcg aagcaggtgc gacccgtatc
    15481 ggaacgtcag ctggggtagc tattttaaaa ggagaattgg cagatggcga ctactgagtt
    15541 gattgaactg gcaattgaaa ccagcaaaca tgcctatgtc ccctattctc actttcctat
    15601 cggagcagtt ttagtagcta aagacggaag tgtttacacg ggagtgaata tcgaaaatgc
    15661 tagctatcct ttgaccaatt gcggtgagag aacagcagtt tttaaggcta tatctgaagg
    15721 ccaaagagaa ttttcagaat tgattgtcta tggtcaaact gaaaaaccga tctcgccatg
    15781 tggtgcttgt cgccaagtga tggctgaatt ttttgaacaa gatctaaaag tgaccttagt
    15841 cgcaaaagat aaatcgacgg tcgagatgac ggtcggggaa ttacttccat actcttttac
    15901 agacttaaac tagtctgagt cgctctctga gtggcacggt ccttgtggcc aaccaatcca
    15961 tacttgcaac atcgttgcac atcttattta ggaggttcag taatgaacaa gaaacaatgg
    16021 ctaggtcttg gcctagttgc agtggcagca gttggacttg ctgcatgtgg taaccgctct
    16081 tctcgtaacg cagcttcatc ttctgatgtg aagacaaaag cagcaatcgt cactgatact
    16141 ggtggtgttg atgacaaatc attcaaccaa tcagcttggg aaggtttgca ggcttggggt
    16201 aaagaacaca atctttcaaa agataacggt ttcacttact tccaatcaac aagtgaagct
    16261 gactacgcta acaacttgca acaagcggct ggaagttaca acctaatctt cggtgttggt
    16321 tttgcccttc acaatgcagt tgaagaagca gctaaagaac acactgactt gaactatgtc
    16381 ttgattgatg atgtgattaa agaccaaaag aatgttgcga gcgtaacttt cgctgataat
    16441 gagtcaggtt accttgcagg tgtggctgca gcaaaaacaa ctaagacaaa acaagttggt
    16501 tttgtaggtg gtatcgaatc tgaagttatc tctcgttttg aagcaggatt caaggctggt
    16561 gttgcgtcag tagacccatc tatcaaagtc caagttgact acgctggttc atttggtgat
    16621 gcggctaaag gtaaaacaat tgcagccgca caatacgcag ccggtgcaga tattgtttac
    16681 caagtagctg gtggtacagg tgcaggtgtc tttgcagagg caaaatctct caacgaaagc
    16741 cgtcctgaaa atgaaaaagt ttgggttatc ggtgttgacc gtgaccaaga agcagaaggt
    16801 aaatacactt ctaaagatgg caaagaatca aactttgttc ttgtatctac tttgaaacaa
    16861 gttggtacaa ctgtaaaaga tattgctaac aaggcagaaa aaggagaatt ccctggcggt
    16921 caagtgatcg tttactcatt gaaggataaa ggggttgact tggcagtaac aaacctttca
    16981 gaagaaggta aaaaagctgt cgaagatgca aaagctaaaa tccttgatgg aagcgtaaaa
    17041 gttcctgaaa aataatggat tgaagtcatt tcttaggaag cggccaaggg ccgctttttt
    17101 aagagttgag acaaagtcat tttgtctcag taaagcttgc tactagacaa actagcaagt
    17161 tttattgaaa taaaataaga ctctgaaagg aagagcacat ggcacacgaa aatgtcattg
    17221 agatgcgtga tattaccaag gtgtttggtg gatttgttgc caacgacaaa atcaacttgc
    17281 acctacgaaa aggtgaaatt catgcacttt taggagaaaa tggggctggt aagtccacgc
    17341 taatgaacat gttagcaggc cttcttgaac caactagtgg tgaaattgcg gtcaacggtc
    17401 aagttgtcaa tctcgactcc ccatctaaag cagctagctt gggaatcggg atggttcacc
    17461 agcactttat gttggttgaa gccttcacag tggctgaaaa catcatttta ggtagtgaat
    17521 tgactaaaaa tggtgtgcta gatatcgctg gagctagcaa agaaatcaag gctctttctg
    17581 aacgttatgg cttagctgtt gacccttctg ccaaggtagc agatatctca gttggagccc
    17641 aacaacgtgt agaaatttta aaaacacttt atcggggggc tgatatcctt atctttgacg
    17701 aaccaacggc tgttttgact ccatcagaaa ttgatgagtt gatggctatt atgaaaaatc
    17761 ttgtcaaaga aggaaaatca attatcttga ttacccacaa attggatgaa attcgcgcag
    17821 tttctgaccg tgttacagtt atccgtcgtg ggaaatcaat tgaaaccgtt gaaattgcag
    17881 gggctaccaa tgctgatttg gcggaaatga tggtaggacg ttctgtttcc tttaaaacag
    17941 agaagcaagc ctctaaacca aaagaagtgg ttttgtctat caaagatttg gtggtcaatg
    18001 aaaaccgtgg tgttccagct gttaaaaatc tgtccttgga tgttcgtgct ggagagattg
    18061 ttggtattgc ggggattgat ggaaatggtc agtctgaact gattcaagcc attacaggtc
    18121 ttcgtaaggt tgaatctggt agcattgagc taaaaggaga ttcaattgta ggcttgcacc
    18181 cacgtcagat tacagaacta agtgttgggc acgttccaga agaccgtcac cgtgatggct
    18241 tgattttgga aatgatgata tctgaaaata ttgcccttca aacctactat aaagaaccac
    18301 atagtaaaaa tggaattttg aattattcaa atattacttc ttatgctaaa aagctgatgg
    18361 aagagtttga tgttcgcgct gccagtgaat tagttcctgc agctgcactc tcaggaggaa
    18421 atcaacaaaa agcaattatt gctcgtgaaa ttgatcgaga tcctgatctc cttatcgtta
    18481 gtcagccaac tcgtgggttg gatgtcggtg ccattgagta tatccacaaa cgcttgattg
    18541 aagagcgtga taatggcaag gctgtccttg ttgtcagctt tgaattggat gagattttaa
    18601 acgtctcaga ccgtattgcc gttatccacg atggtaagat tcaaggtatt gtatcaccag
    18661 aaacaaccaa taaacaagaa cttggtgtct tgatggctgg tggaaacttg ggaaaggaga
    18721 agagtgatgt ctaaaaaatt acaacaaatt tcggttccct tgatttctgt attcctagga
    18781 attttactcg gagccattgt catgtggatc ttcggttatg atgctatttg gggctacgaa
    18841 gaattgttct atacagcctt tggcagtctg cgtgggattg gagaaatctt ccgtgctatg
    18901 ggtcctctgg tcttgattgg tcttggtttt gccgttgcca gtcgagctgg tttctttaac
    18961 gtcggacttc ctggtcaggc tttggcaggt tggattctca gtggttggtt tgccctgtcg
    19021 catccagata tgccccgtcc cttgatgatt ctagcaacca tcgtgattgc cttgattgct
    19081 ggtgggattg tcggagcgat tccaggtatt cttagggcct atctagggac gtcagaggtt
    19141 attgtaacca tcatgatgaa ctacattgtc ttgtatgtag ggaatgcctt tatccatgct
    19201 ttccctaaag acttcatgca aagtacagat tcgaccattc gtgttggggc taatgcaacc
    19261 tatcagacac cttggttggc tgagttgact ggtaactcac ggatgaatat tggtattttc
    19321 tttgccatca ttgccgttgc agttatttgg ttcatgctca agaaaacaac tcttggtttt
    19381 gaaatccgtg cagttggtct taatccacat gcttcagaat atgctggtat ttctgccaag
    19441 cggactatta tcctatctat gattatttca ggtgccttgg caggtcttgg tggagctgtt
    19501 gaaggtttgg gaaccttcca gaacgtctat gttcaaggtt cgtcattagc tatcggattt
    19561 aacggaatgg cggttagttt gcttgcggcc aactcaccaa ttggtatact ctttgcagcc
    19621 ttcctatttg gcgttctcca agttggggct cctggtatga atgcggcgca ggtaccatct
    19681 gagcttgtca gcattgtaac agcgtctatt atcttctttg tcagtgttca ttaccttatc
    19741 gaacgctttg tcaaaccgaa aaaacaagtt aaaggaggta agtaaagatg tctattataa
    19801 ccttgctccc cctcttggtg tcttctatgc tgatttactc agcacctctc atctttacaa
    19861 gtatcggtgg tgttttctct gaacgtggtg gtgtggtaaa cgttggcctt gaaggaatta
    19921 tggttatggg tgccttttct ggagttgtct ttaaccttga atttgcagaa caatttggag
    19981 cagcaactcc atggctatcc ttgcttgtag caggattggt tggtagtgtt ttctctatca
    20041 tccacgcagc agcgacggtt catttccgtg cagaccatgt tgtcagcggt acggtattga
    20101 acttgatggc gcctgccttg gctgttttct tagttaaagt tctttataac aaaggacaaa
    20161 ccgacaacct aagtcaaact tttggacgct ttgatttccc agtcttggca aatatcccag
    20221 tgattggtga tatcttcttc aagtcaacta gtctacttgg ttatctggcg attgccttct
    20281 cattccttgc ttggtttatt ctctttaaga ctcaatttgg tcttcgtctc cgctctgtcg
    20341 gtgaacatcc tcaagcagcg gataccttgg gaatcaacgt ctacaagatg agatatttag
    20401 gggttattat ttcaggtttt ctaggtggaa ttggcggagc gatttatgcg caatcaatct
    20461 cagttaactt ctcagtgaca actattgttg gacctggatt tatcgccctt gctgcgatga
    20521 tctttgggaa atggaatcca atcggtgcta tgctttctag tctcttcttt ggactttcac
    20581 aaagtttggc tgttatcggt tctcaattgc cgttcctaca aggagtgcca gcggtttatc
    20641 ttcaaattgc accttatgtt ttgacaattc ttgtcttagc agccttcttt ggaaaagcag
    20701 tcgcaccaaa agcagatggt atcaactaca tcaaatcaaa ataagcatac aaaaaaacgt
    20761 cagtccaatg actgaactac accccaaaag ttagatagaa aaaatctaac ttttggggtg
    20821 tttttatta
//
LOCUS       DAVGJJ010000031        19186 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000031 DAVGJJ010000000
VERSION     DAVGJJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.031"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(41..694)
                     /gene="phoU"
                     /locus_tag="VVE35_001029"
     CDS             complement(41..694)
                     /gene="phoU"
                     /locus_tag="VVE35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU3061239.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(706..1464)
                     /gene="pstB"
                     /locus_tag="VVE35_001030"
     CDS             complement(706..1464)
                     /gene="pstB"
                     /locus_tag="VVE35_001030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3061240.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(1477..2280)
                     /gene="pstB"
                     /locus_tag="VVE35_001031"
     CDS             complement(1477..2280)
                     /gene="pstB"
                     /locus_tag="VVE35_001031"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU3061241.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2291..3175)
                     /gene="pstA"
                     /locus_tag="VVE35_001032"
     CDS             complement(2291..3175)
                     /gene="pstA"
                     /locus_tag="VVE35_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU3061242.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3165..4082)
                     /gene="pstC"
                     /locus_tag="VVE35_001033"
     CDS             complement(3165..4082)
                     /gene="pstC"
                     /locus_tag="VVE35_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU3061243.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4082..4960)
                     /gene="pstS"
                     /locus_tag="VVE35_001034"
     CDS             complement(4082..4960)
                     /gene="pstS"
                     /locus_tag="VVE35_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU3061244.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5534..6838)
                     /locus_tag="VVE35_001035"
     CDS             complement(5534..6838)
                     /locus_tag="VVE35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU3061245.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6839..7612)
                     /locus_tag="VVE35_001036"
     CDS             complement(6839..7612)
                     /locus_tag="VVE35_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU3061246.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVDEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7602..7880)
                     /locus_tag="VVE35_001037"
     CDS             complement(7602..7880)
                     /locus_tag="VVE35_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU3061247.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7880..8281)
                     /locus_tag="VVE35_001038"
     CDS             complement(7880..8281)
                     /locus_tag="VVE35_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU3061248.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8491..9108)
                     /locus_tag="VVE35_001039"
     CDS             complement(8491..9108)
                     /locus_tag="VVE35_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU3061249.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(9098..9871)
                     /locus_tag="VVE35_001040"
     CDS             complement(9098..9871)
                     /locus_tag="VVE35_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU3061250.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRGIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(9882..10619)
                     /locus_tag="VVE35_001041"
     CDS             complement(9882..10619)
                     /locus_tag="VVE35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU3061251.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSAGVINAQAIITWQEIKKA"
     gene            complement(10624..11754)
                     /locus_tag="VVE35_001042"
     CDS             complement(10624..11754)
                     /locus_tag="VVE35_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU3061252.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11838..12227)
                     /locus_tag="VVE35_001043"
     CDS             complement(11838..12227)
                     /locus_tag="VVE35_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU3061253.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12243..12626)
                     /locus_tag="VVE35_001044"
     CDS             complement(12243..12626)
                     /locus_tag="VVE35_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU3061254.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12627..13415)
                     /locus_tag="VVE35_001045"
     CDS             complement(12627..13415)
                     /locus_tag="VVE35_001045"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU3061255.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13408..14343)
                     /locus_tag="VVE35_001046"
     CDS             complement(13408..14343)
                     /locus_tag="VVE35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU3061256.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14473..14649)
                     /locus_tag="VVE35_001047"
     CDS             complement(14473..14649)
                     /locus_tag="VVE35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU3061257.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14729..15238)
                     /locus_tag="VVE35_001048"
     CDS             complement(14729..15238)
                     /locus_tag="VVE35_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061258.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSEADMEEMV
                     NYIINHYEE"
     gene            complement(15316..16023)
                     /locus_tag="VVE35_001049"
     CDS             complement(15316..16023)
                     /locus_tag="VVE35_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU3061259.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKVEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            16174..17202
                     /gene="queA"
                     /locus_tag="VVE35_001050"
     CDS             16174..17202
                     /gene="queA"
                     /locus_tag="VVE35_001050"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU3061260.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(17863..18252)
                     /locus_tag="VVE35_001051"
     CDS             complement(17863..18252)
                     /locus_tag="VVE35_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU3061261.1"
                     /translation="MVKRRIMRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(18306..18989)
                     /locus_tag="VVE35_001052"
     CDS             complement(18306..18989)
                     /locus_tag="VVE35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU3061262.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVGEIG
                     KLLLLQSIL"
BASE COUNT         5670 a         4400 c         3479 g         5637 t
ORIGIN      
        1 agttgatttc aagctccttt ttatacagaa ttaaactatt ttatagttcg acaatcttac
       61 ctgtttcaaa gtagacaacc cattcacaga tatttttagc atagtcaccg atacgctcca
      121 agtaggaaat aacttggaaa taatcacgac ccgtaacaat ggcttctgga tttttcttaa
      181 tctcttcagt cgcaaggtca cggatagttt caaaatagtg gttaatttgc tcatccatgg
      241 aggccacccg gtatgcgtcg tcaacagaac cattaagata aagatcaagt gctgcttcaa
      301 caacgctttt aacttcacgt cccatttttt taatttcttc ctctacagct ggaatgcgtt
      361 cttccccctt catacggatg gttgcctggg caatggctac agcgtgatcc cccatacgct
      421 ccacatctga tacagcctta aggacagtca agactgtacg caaatcttga gagactggtt
      481 gttggagtgc gatcatttca aatgatttct tttccagttt cacttcgtat tcatttactt
      541 ctgcatcatc ttctatgacc tcttttgcca ggtcacggtc atgcgtgaca aaagcacgta
      601 ccgtacgatt gatttgtgag agcacttctt gtcccatagc gtagaactgg ttatgtaatt
      661 tctctaaatc ttcttcaaat tgagatcgta acatctttca tctccttatc caaattttcc
      721 tgtaatatag tcttccgttt ccttgtgttg gggatcaagg aacatctgct tggtatcatt
      781 aaattcaatc aaatctccat ctaggaaaaa tcctgtctta tcagagatac gtgaagcttg
      841 ctgcatggaa cgggttacca gaagcatggt gtacttgtct tttagaccat acaaggtttc
      901 ctcaatttta ccagctgaaa tcggatccaa agccgaagtt ggctcatcca agaggatgat
      961 tttaggacta gttgccaaga cacgggccac gcagacacgc tgctgttgac cacctgacaa
     1021 tccaatagct gaatcatata gacgatcctt gacctcatcc cagatagagg caccttgcaa
     1081 ggctttttct acggcttcat ccagaacctg cttatcctta attccattga tacgaagccc
     1141 gtagacaaca ttctcataga tagtcatagg gaaaggatta ggttgttgga aaaccattcc
     1201 gatttcctta cgtaattcaa ccgtatctgt acgcggactg tagatgttgt gaccattgta
     1261 caccacggat ccagttgtgg tcacctctgg attgagatct cccatgcggt tgagagactt
     1321 gaggagggtt gacttccctg atccagatgg accaatcaag gctgtaattt ccttaggttg
     1381 gaaagatagg gaaacactat tcaaagcctt ctttttatta taataaacgg acaggtctga
     1441 tacctgtaaa atcgcatctg tcatacggtt tcctttctaa ccaaagtgac cagatacata
     1501 gtcattggtg gactgtagct tagcattttg gaaaatagtt gcagtcttgt catactcaat
     1561 caaatcaccc aagtaaaaga agcctgtata gtcacttgca cgagcagcct gctgcatatt
     1621 atgcgttaca atgatgatgg taaagttttt cttgagctca aacatggtct cttctagttg
     1681 catggtcgca atcggatcca aggctgaggc tggctcatcc attaagagga tatctggctt
     1741 aacagagatg gcacgagcga tacagagacg ttgttgctga ccacctgata aggtcaaggc
     1801 tgacttgtgg agatcgtctt taacctgatc ccagagggca gcctgacgaa gggaggtttc
     1861 tacgatttca tctaggactt gcttatcctt aactccagca cgttcatgcg caaaggtaat
     1921 attacggtaa attgacttag caaatggatt ggggcgttga aaaaccattc caatgtgttt
     1981 acgcatttca taaacgttga tttctggacg gttgacatca attccacgat agagaatctg
     2041 cccagttact ttagcaatat caatggtatc attcatgcga ttgagactgc gtaagtaggt
     2101 agatttcccc gatcccgacg gaccaatcaa agctgtaatt ttatttcttt caaattgcat
     2161 atcaatcccc ttaatggatt catttttacc atagtaaaca tggacatcct tagtagaaag
     2221 ggctactttt tcttcaggaa aggtaaggat atgcttctca tcccagttat atgttgacat
     2281 ggcttctcct ttaggcagcg gttaatttct tgtgtagata gcttccgaac ttacgagctc
     2341 caaagttaaa aatcaggata aagatcagga gcacagcggc agaacctgct gatacaatgg
     2401 ttccatctgg aatagtgcct tcactattga ctttccagat atggacagcc aaggtttctg
     2461 cttgacggaa gatagagatg gggctagtca cactgaggat attccagtta gaccagtcaa
     2521 gagctggcgc cgattgccct gctgtataga tcagagctgc agcttcgcca aagatacgac
     2581 cagatgccaa gacgacaccc gttacaatac ctggaagcgc ttctggaata acaacatgaa
     2641 ccactgtctc ccagcgagaa atcccaagag ccagaccagc ctcacgttgg gtatggtgaa
     2701 cgtgtttcaa actatcctct acattacgcg tcatctgagg caagttaaag actgtcaagg
     2761 ccaaggcacc tgaaatgatt gaaaatccat actcaaactg aactacaaag atcaagtaac
     2821 caaagagacc caccaccact gatggtaaag aggacaagat ttcaatacaa gtccgcacaa
     2881 agttggtaac aggacctttt ttagcatatt cagccaagta aatcccagct cccatagaaa
     2941 gaggtacaga aataatcaag gtaatgacca ataggaaaaa ggaattgtaa agctgaatgc
     3001 caatcccacc acctgcttga aaagcagaag accttccagt caagaaagac caagagatat
     3061 ggggcaagcc ccgaaccaag atatagagaa tcaaggaagc caagattgtc acaatgatgc
     3121 tagcaatcgt atagaggaca gctgttgcaa gtttatctaa tttcttagcg cgcataattt
     3181 ttctttcctc tttctttcgt aatcaattta atcacactgt taaaaactaa actcatcaag
     3241 agcagtacca aggccagtga ccagagaaca ttattattta cagttcccat gacagtgttc
     3301 ccaattccca tagttaatat agaagttaaa gttgcagctg gtgtggtcaa ggaagttggg
     3361 ataacagctg agtttccgac aaccatctgg atagctaaag cctcaccaaa ggcacgcgcc
     3421 atcccaaaga ccactgcagt gaaaatacca gaacgggccg ccttcaagat cacacgccag
     3481 atagtctgcc agcgagtggc tcccatagcg aaactggctt cacgataata acgaggaacc
     3541 gcacgcaagc tatccgttgt cataaaggtt acggtcggca aaatcatgac aaagaggacg
     3601 gaaatccctg acaaaatccc aaaaccagtc ccaccaaaga cactgcgaac aaagggaacg
     3661 acgacttgca agccaataaa tccgtacact actgaaggaa tcccaaccag gagttcaata
     3721 gctggttgca aaatcttcgc cccttttggt gatacttcgg tcataaaaac tgctgcacca
     3781 atagcaaagg gggttgcgat aagggctgag agaatggtaa cgataaagga acccaaaatc
     3841 ataggaaggg caccaaattc tttactagaa ggattccaag ttcctcccaa aagaaagtca
     3901 aagatattca caccattgac aaagaaggtc gacaagcctt tttgcgctac gaaaaccaaa
     3961 atcatggcca caaggatgac tatcaaagaa agacaggcaa aggtcaaacc ttttcctaat
     4021 ttctccagac gagaattctt tgatggaagc aacattttct tagctaattc ttcttgattc
     4081 attattgtct cccttccaac actgtcacag ttccggcagc atctttttca accttcattt
     4141 ccttaatcgg aatatacttc aatcctttga caatcccttc ttgggtctca tccgagagaa
     4201 caaaattgag aaattctgca gccaactcat tgggctgccc caatgtatac atatgctcat
     4261 aagaccacaa gggccaatta ttgctactta tattttctgg acttaagtca tagccattca
     4321 acttcatgct tttgaccgaa tcatctatat aggtaagaga taaataagag atagctcctg
     4381 gactttttga tacgattgat tttaccgctc catttgaatc ctgctcctga ctttgcatgg
     4441 cagactgacc ttccataatg acagtatcaa aggtagcacg agaaccagag ccggctgccc
     4501 gattgataac agagatgggt aagtccttac caccaacctc tttccaattg gttacctcac
     4561 ctatgaagat ttgacgaagt tgctctgtcg ttaggttatc aacatcaacc tccttattga
     4621 caatcagagc caagccagct accgcgacct tgtggtcaac aagagcagaa gcatcaattc
     4681 cgtctttttc ctcagcaaat acatctgagt ttcctatatc aactgcccca gactgaacct
     4741 gggacaagcc tgtaccagaa cctccccctt ggacattgac cgtttttcca acatggatcg
     4801 tgccaaattc atctgccgct acttcaacca agggttgcaa ggcagttgag ccaacagccg
     4861 ttatggattc tccacgatca atccagctag cacagcctac taaacaagcc gtcagccaaa
     4921 aagcgataag agacagagca agcttttttc tttttttcac tgtttttctc ctcgaaaata
     4981 attatgaata ctgtgaattt tttaagtagt tctttatgag ttgacgcacg aattcttacc
     5041 aaatttctgc gcaattgatt atttatataa tataggctat attactcttt cctaacctcc
     5101 ttttttcata tgtggataaa atctcttgtc tatcccttcc cccattgtca cccattatag
     5161 tcatttcgtg tctctttttc ccctttttaa tgcaagggaa attactctcc ttagatgata
     5221 atccaaaagc tagaaaggta tctcaaacct ctctactctc ccagactagt ttacaactaa
     5281 aaggaaaaga ttctatttta tgagaaatct agtttacaag cggtaagaac gctaataact
     5341 aaacttcttg tactctttga aaatctcttc aaaccagtgt tttgagctat ctatggctag
     5401 cttcctagtt tgctctttga ttttcattga gtagtaaaac tacatgtaat ggcaatcaag
     5461 atatcaagaa tcatcctact aaaaaaatcc atactttcac tataacatag aataagatat
     5521 ttgactagca ttttcatttg aatctgaggc cttttggaaa ataatttttc aaaacatttc
     5581 cagtaacctt tgcaaagccc aagccattgc ctttaaccaa aacttggtac caaccatttg
     5641 gtagactttc tgccagctga acgatttctc cagccgcata cttgacaaac gcttcttggc
     5701 caatttcaac cgactgttcg acctgactcg atttcaaggc taaaccaaga gcgaaactgg
     5761 gctcaaagcg tttcttctta aaagtaccca gatgcagtcc attgcgagca atcttgagct
     5821 tccctaaatc tggcaaaagt tctggcaaga gataaagctg gtctccaaaa gtctgcaaga
     5881 tacccggtag attgaccttc aaatggtttt gggcaaattc ctgccacaag gcaacttgtt
     5941 cacggctgag gttactctta cttgccttaa atttaggagc tggattgtta cccttaaact
     6001 gtagatgggc aacaaactga ccctctccct taaactgatg agggtacatc cgagccgttt
     6061 ctggcaggtc aataccagct accattccat tgatatgctc tactggcaac aagtcaaaat
     6121 catactcttc cagcaaccaa ttgacaatct cttcgttttc ctcgggtgcc caggtacagg
     6181 tcgaataaac cagacgacca ccttcagcta acatggtcac tgcatcctcc agaatttctc
     6241 tttgcaagct agcacattga ctcggataat ctaagctcca atagtccata gcatcaggtt
     6301 gcttacgaaa cattccttca ccagagcaag gggcatcaag aacgattaag tcaaaatagc
     6361 ctttaaagac cttgaccaag cggtcggcag attcattggt caccacgaca tttgtcgctc
     6421 caaaacgctc catgttttca accaaaatct tagcccgttt gcttgaaatt tcattggaaa
     6481 caagtagccc ctcccctgct agataggctg ccagttgagt tgatttgccc cccggtgcag
     6541 cagccaagtc caagaccttc ataccaggac tgggttgggc tacttgagcc accatttgag
     6601 cagcaggttc ttgcgaataa actaaacctg tagcatgctc aggcgatttc cctgaaacct
     6661 tcccatagtg gccccaaggg gtttgagtaa tggcatcaga aaaggaaagt tgctcttctt
     6721 ttaagggatt gacccgaaag gccgaaaccg cttcctcctc aaaagaggca agaaaatctc
     6781 ttgcctcatc tcctagtatt tctttatatt tttcaacaaa tccttctgga aatttcattt
     6841 aagttctttt cctttcgtaa atataggact gaatttcctc ctgcatctca agaggcacca
     6901 tcatgaccgg ctgtctggtt tgaaaatcag gagcttcacc aaaaagggtc acaacccgat
     6961 agcccagact ttcccctaaa atactagctg cggcataatc ccatggttgc agataggtga
     7021 gataggtcaa caaacgccct gacaaaatct tggcaaaact aatggccgca cttccataga
     7081 cacgaacacc aagaaccgct cggctcaaat cagccagccc ccattcattg gtttccagca
     7141 taccactatt ccctgcaatg agaaaatctc caagtggttt agttttaaaa ggagctaggg
     7201 gcctatcatt tagacaaact ggaaattccc caccaccgtg gtaacaatcc cctttgacca
     7261 catcataaat cagaccaaac tgtccctgac cattttcaaa ataagccatc ataacagcaa
     7321 aatcttcctg ctgggctaca aaattattgg taccatcaat gggatcaatg acccaaacct
     7381 tgccctcttg aaccgaggct cgcagacaac cttcttcagc acaaatctta tcctcaggat
     7441 aacgggacaa aatctcatca accaagagtt cctgaacttc tttgtccagt ctggtcacca
     7501 aatctgttgg agaggacttg gtttcaacac gcaagtcttc ctgcatatgg tcaagaatgt
     7561 actggcctgc tttcttaaca agctctttag caaattcaaa tttactttcc aagagaaatc
     7621 tttccttccc ctttttcttt ggcagcctga actgctcggt aaagtgaata gccactaacc
     7681 gtttcaaatt ctcgtcccaa acgtttctct tcgctcttgc ttggcacgac cgctttgaat
     7741 cccttatagg agtccagtaa ctttttagcc tctactttgc cttcataggc agcttcaaca
     7801 tcattaaaaa aagaaagcac tgaagcaagt tcttcagtgc tccacgacaa atctagtggg
     7861 taactatact gtttgttcat taactaatac cagctctcat tcttgcttct tttagttctt
     7921 gcttacgata actacgaggg agaaaagcac gaatctcatc ttcattaaaa ccgatttgca
     7981 tacgcttggc atcaataata attggacgac gcaaaagact aggatactgc tcaatcaaat
     8041 gaagcaattc cgataccgaa atactctcta catcaatatt caatttttga aaaatttttg
     8101 aacgagttga aatgatgtca tcagtaccat tttcggtcaa ggaaaggatg tgttgcaatt
     8161 cttttcttgt taaaggactg gtcataatat tgtgttccac aaagggaacc ttatgttttt
     8221 ctaaccaggc cttagcctta cgacatgatg tacagctcgg tgatagaaat agtgtaatca
     8281 tgcttttctc ttcttatcta tactttgcta cttctattat acaaaaaaat aaagcgcttg
     8341 actagggatt tttagaaaaa aagcctattt tttcaagaaa aataggcttt ttgcgaacga
     8401 ttgacacaat tggatttggt taattcactc ttaacgatgg ttttaaacga tatatatttt
     8461 tatatatgta aattaaaaac atctttcctt tcacttccta cgacttttca gatacagata
     8521 gccaaagaag ttttcataga gggcaaaaaa gaggaggaag gcatgaagaa agaaggtctc
     8581 tggcaaaatc ataataacag gatccttggc tggatcaaaa agccaggtat catctcccac
     8641 aaagagaatt tgatggaaaa gagtaaagaa ttggtcaaaa ccaatcaaaa ctcccccaag
     8701 tccaatcatc acaggtaaga ctactagagc caggagactt tttcgataaa gagacaaaaa
     8761 gtcctttttc acaatcctat tgacaaagac atagaaactt ggcagtgtca ctagagctac
     8821 tagctgaacc aaatgaaaga gattcttgac cactgcgaaa tggtgcagac cagctgctga
     8881 cgaacgaaaa tcaggcatct gtaagacctg actaaaagga ttggtcagat aattcatcaa
     8941 gatatgaaaa ttgtattgaa tggtttctgg ttttagatag actcgattcg ttaagtttag
     9001 ccactgaatc tccataggat agaaaatcca agccagataa atagtcagaa ggattgagag
     9061 ggagaggaga aagagcatag agccccaaaa ggtcaattta gttttcatca aaatcccact
     9121 ccgcaaggct agaaaccaca tgtgtcggtg cgattggcag gccagctact tcttctgcct
     9181 tagtaaaacc tgtcgtcacc aagagcgttg gaatgccatt gtcaatccca gcccggatat
     9241 cagtcaaata attgtcccca accatgatta actcttcacg ttccaaacct aagtgctcaa
     9301 ccgccttgtc cataatgatg gcatttggtt ttccgatata aaccggcttc actcgtgtcg
     9361 ctacttcaag tagcgtaatc agtgagccag cacctggcaa aagaccacgt tccgtcggga
     9421 tgttgaggtc aggattggtt ccgataaaat gggcaccctt ttgaatagca agagttgctg
     9481 tggcaaattt ttcatagtcg acttgccaat ccagaccaac taccacgtag gcaggttttt
     9541 ccttgtcttc cacataacca gccgccttga tggcttcctt gagtcctgct tctccgacga
     9601 catagacggt cttttcaagc cccaaatcat tcatatagtc gatggttgcc aaagtcgctg
     9661 tgtagacagt cgataggggc gtatcgatat taaaattctg agccaacatc tccttaacac
     9721 tctctggagt gcgagttgta ttgttggtta caaagagata gggaatgccc cgcttttgca
     9781 attcatgaac aaaagtctct ccagcaggga ttcggtcttt ccccttataa atggttccgt
     9841 ctaaatcaat taaatagcct ttatatttca tctatttctc cctaagcctt ttttatttct
     9901 tgccaagtaa tgattgcttg ggcattgata accccagcac ttgtaatttc atgcttgctt
     9961 tccagtccag tccgttcaac agccgatgta atcaccccac ctggtcgaac ttccttgaca
    10021 tacttgaggt tgattttctt gggaatatag tgggtcaaaa aatccgctcc catgacctca
    10081 aaaatccagt ccaagtattt actgttattg acatgaccat tcatatccaa gtcgtaaaaa
    10141 cgaacatggt aatccttgct gatcggctct tccaaggatt catacttcgg tccacggata
    10201 agttttttat caaaatcaga ctggtaagga gccacaatct caggttcaac agcatggact
    10261 tttcgactgt cgcggtccat gagaacaaag gtcgccatca tatggatgag ctcctgcccc
    10321 gcttcattat aaatagtaaa gcgacggtag caaaaaagtc gattgtagct caaggcttcc
    10381 gtttcgatgg taatttcttc cgcaaaacga ggcaaacgaa ccacctcaat atcatattct
    10441 gcgataatcc agaccagatt atagtcttcc aaaatggcct tatcactaac tcccagttca
    10501 atcgactgca tccctgaaac ttgcagtgac agcaaaatca catctggaag tttgatatga
    10561 ccgttcatat cagccatatc aaaaggaatt ttcattttca tttgataagt taagcccatg
    10621 atcctactcc aaaataaatc gttctgctac agtatctccc aaaaagagac ctctctttgt
    10681 catgcgaacg tggtcaccct caatctgcat gaggccttgt tgaaccaaat ctctgacaat
    10741 ttctccataa agtccagcaa aagactgtcc aaatttttcc tcaaatcgcg ccatggaaac
    10801 cccggatttc ttgcggagtc ccaagaacat ttcttcttcc atttgctcct tttgactcag
    10861 gtgatcttct gtaatacaag cattgccttc ctcaaccgca ctgagataat gacgaatggg
    10921 accatgattt ttatagcgta ctccattgac ataaccagat gcccctgcac caataccata
    10981 gtattcagca ttgtcccagt acatgagatt atgacgactt tcaaagccgg gtttggagaa
    11041 attagaaatc tcataatgct caaaacccgc tcgctccagc tctgcaatga tgtactcaaa
    11101 catctccgct tctagttcct ccttaggcag aggcaatttc ccacgtcgca tccggttcat
    11161 aaagaccgta tggttttcta aaatcaaact atacaaactc atgtggggaa tatccaatcc
    11221 aatggcttta gccacatttt cctttacttg ctccatggtc tgaccaggca gagcataaat
    11281 caaatcaatg gagatgttgt caaaaccagc cagtttcagg cgatcgatat tttcataaat
    11341 atccttctcc aaatgactgc gcccaatctt tttcaacatc ttatcatcaa aggtctggac
    11401 acctagcgaa acacgattga cagccgaatt tttcaaaaca gctatcttat ccgcatccaa
    11461 atcgcctgga ttggcttcaa tggtcaactc ttccaagaca gacaaatcca agtttttagt
    11521 caagccattc agtaacacct ccagttgcgg agccgacagg gctgtcggtg ttccaccacc
    11581 gatataaagg gttgacaact tttcaatatc ataagaacga aactcttcca gcagatgctc
    11641 taaatagctg tcgactggct gatttttgat gaagaccttt gaaaaatcac aataataaca
    11701 aatctgggta caaaatggga tgtgcacata ggctgacgtt ggttttttct gcatagtaat
    11761 tattatacca caaagactag attccagata aaaatcacca tccccagata catagtccgt
    11821 ccggagatgg tgatggttta ttcttctgtt atatcaatca caatctcttc tgagtcatca
    11881 agagcttcgg ctttttcttg ccattgctcc ttgagattat ttaattgatt ttttgatgct
    11941 tctgtcgctt gaaaagcata ggatttagct tgagcaagta tactgtccac agtgatttca
    12001 cctgactcaa cctgttcttt tgttttcaga acaaaatctg tagcctgctc cttaacttct
    12061 gtcagttttt cacagacttg ctccttggca tactccggat cttctctcaa atcatctaga
    12121 aaatcttgag cctgactgca aacttgtttg cccttatcac ttgttaaaaa caaggcaaga
    12181 gctgcacctg aaaccgttcc taaaaggatt gaggataatt tacccataag gattctcctt
    12241 ttttattttt tgaaaaattt acttgcaaga cgaagagctg acagacttgc accagtcttg
    12301 agtgtttttg aaccagctga tgaagctttc ttgctcaaga cacgcgcatg gtcattgagg
    12361 tctgaaacag atagagataa atctgcaaca gcactgaaga gtggatcaat cgtagccacc
    12421 ttgacattga tatcatctgc caagacattg accttagcca acaactcatt ggtgtgatgc
    12481 aaggtcacat ccacatctga agtcaaggtt ttaatcgtct tttctgtttc atcgatgaca
    12541 cgaccaagct tttgtacagt aatgatcaga tagaccaaaa agacaatcaa agctagggca
    12601 acaagaatat atgcaacttc taacatttag ttttcctcct ctgtaatata gtaaggggcc
    12661 ttctttcgat tttgataaat aacgatcatt ataccgagac cgataaggac aactgacagc
    12721 cattgggaca ctcgaagtcc gaagaacatg agactatctg ttcgcatacc ttcgatgacc
    12781 atacgaccga aaccatacca aatcaagtaa aaggccgtga tatgacctcg tctgagactc
    12841 ttccatttcc gtctaaaaat cagaatcaag gcaaagccaa gcagattcca tagagactca
    12901 taaaggaaag tcggttgacg gtagctcccc tcaatataca tctggtcacg gataaagcca
    12961 ggtagataat ccagattatc cactgttgca ccataagctt cttggttaaa gaaattaccc
    13021 caacgcccca aactttgagc aatcataacg ctaggcgccg caatatctag aaaatcccaa
    13081 gtattgatga gtttacggtc agcaaagata tagagcacaa gagccccagt tatcaaacca
    13141 ccgtaaatgg ccaaaccacc attccaaatg gcaaaaatct ctcctaaatt ctgactatag
    13201 taatcaaatc ggaaaataac atagtagaga cgagctccta aaatagccaa gggaaaggct
    13261 actaagataa aatctaaaat atcgtctggt atgatcttct ttctaggtgc ttctttcatg
    13321 gtcaaataaa ccgcaagaat caagcctgtc acaatacata aggcatacca acgaatggct
    13381 aggggtccta gttgaatagc aattggatca agcattttgc acctcatttc gagcgatgag
    13441 acttgtcagt cgttcgtcga acaaacgggt cgcatcaaag cccatttcct tggcacgata
    13501 attcatggca gctgcctcaa tcacaacaga gatattacga cctgttttaa ctggaatacg
    13561 aatacgagga atggctacgc cagaaacttc aagttcctct gcattgtttc caaggcgatc
    13621 aaaggtctta tgcgtatcgt aattttccaa atagacagca agctgaacct gtgaagaatc
    13681 cttgacagca ctcgcaccgt agagactcat aacatcgata ataccaaccc cacgaatttc
    13741 aatcaagtgt ttcaaaattt cagctggttc accccagaga gtaatctcat ccttggcaaa
    13801 gatatcgaca cggtcatcgg ctaccaaacg gtgaccacgt ttgacaagct caagacctgt
    13861 ctcgctctta ccaattccac tatctccctg aatcaagacg cccatcccat aaatatccat
    13921 caagacaccg tgcacactgg tacgttctgc caaacgagaa tccagatagc tagataactc
    13981 tccagataaa cgactggtag ctgcacggct ggttaaaata gcaatcttac attctctagc
    14041 agcctttaac atctcctctg gaaccaccaa accacgggca acaatgactg ctggtgtctc
    14101 aggtagaaac atttttttca aaacttcata acggctgttg gaaggcatgc tgatcagata
    14161 agaccactcc ttcatcccca aaagttgaat ccgctctggt gtatagtagt caaaatagcc
    14221 tgtcatttca agaccaggtc gcgtaatatc cgctgtattg atttcctttt caagcaactc
    14281 tggttcacca tagacaatat ctagtctaag cttttcaatc acttctttta ctaaaaccga
    14341 catcatttcc tccttcaaat gagttcttct ctctattata tcaaattgta ggaggaagtt
    14401 tcagattttt gcaggctttg gagtatttat ttaaaagaaa aaggactaga tttctctagc
    14461 ctttcatttc aattagaatt ttttacgttt acgagctact tctgatttac gtttacgttt
    14521 tacagaaggt ttttcataga attcacgttt gcgtgtttct tgaagagtac cagctttagt
    14581 aaccgcacgt ttgaaacggc gaagtgcgtc gtcaagagat tcattcttac gtactactgt
    14641 tttagacatt tttttcactc ccttcaagtt caagatctat tctattctac acgtaaaagg
    14701 aataattgtc aagaggaaac taagtttttt attcttcata atgattaata atatagttca
    14761 ccatctcctc catatctgct tcagataaag gtaattccca gtttgttgtt tccattttaa
    14821 gaaaagcatc actgacttta gttattgtat agcaatcttt aaaaattgga atttttttag
    14881 attgttcaaa taagatgcct attctatcat ataagacgtg aagttcttta ctatttggat
    14941 caatatcaaa agactgtaaa gagattaaga tgtcatttcc tttatatgac aaatcaatcc
    15001 acatgtgatt tgataaatca tatggcctag ataatctact acccgaggtg tactttttat
    15061 ttcctccgcc tcctgaatat tgatttcttg tggtatatgg tttatctttt gacttagtaa
    15121 aaagaaaatt cccctcctta tctttctttt ttgtaatgaa gcctattata ctttttcttt
    15181 cttccgttct agcactctta tatgcttctt ttatttttaa aaattcttct ttattcatgt
    15241 gtcctcctaa agttaagttg aagttttata tgtctaaaac cactcactcc aaagagcaag
    15301 cggttcttat actttttatt tttcgagtaa gctaagcgct tcagcatctg cgatgatggt
    15361 tacatcaggg tgattttgga ggctacttgc aggtaggttc tcagtcactg ggccagatac
    15421 tgttccggca atggcttcta ctttcgactc accgtaagca aaaagaataa tagacttggc
    15481 atccaaaatg tttttaatcc ccattgaaat agcttgggtt gggacgtctt caatcttggc
    15541 aaagaagcgt gcattggctt cgatagtaga ctggtcaagt tctactagat gcgtttgact
    15601 gtcaaatgga gtaccaggct cattaaagcc gatatgccca ttgcgaccga ttcccaagat
    15661 ttggaggtca actggatggt cagccaaaat ttggttgtag cgttcaactt cagcttcagc
    15721 attatcctta acaccacgag gcaagaaact ttctttaaat ggtttttggt tgaacaagtt
    15781 ttcttgcatg aagtaacgat aagactgtgg attgtcccca tcaaggccca catactcatc
    15841 aaggttgaca ctggttagat ttgaaaaatc aaggtcactc tcaacaattt ccttgtaaaa
    15901 ttcaagtggg ctacttcctg tcgcaagtcc tagagtttga gctccattag ccaatttttc
    15961 cttcaaaatc tcaaaagcaa cttttccacc ttgaacttgg ttttcaactt taataacttt
    16021 catcttttca tcctccattt ctgtctatat ttattatatg gtatagacca atttttgtca
    16081 agtgaaaacg atttaatttc taaaaaaaga gcgtccaaac ttgaaacgtg ttataatgga
    16141 taggattaga acttagaaag aatgaacaaa tatatgaata cagctgattt tgatttccac
    16201 ttgccagagg aattgattgc ccaaacgccc cttgaaaaac gtgatgcttc caaactcctc
    16261 atcgtcaacc gtgagacagg agaaatgcaa gataaacact tccactctat tattgatatg
    16321 ctggaacctg gtgatgccct tgtcatgaac gacacccgag ttctccctgc ccgcctctat
    16381 ggtcaaaaag tggagacagg aggtcatgtg gaacttctcc tccttaagaa cactagtgga
    16441 gacgagtggg aagttctggc taaacctgcc aaacgcctca aggtcggtac tcgtatcagc
    16501 tttggtgatg gccgcctcag cgctgtcgtt acagaagaat tgacccacgg gggacgcatt
    16561 gtccgctttg aataccaagg aattttccta gaagtcttgg aaagtctggg agaaatgcct
    16621 ctgccacctt atatccacga aaaattagat gaccgtgaac gttatcaaac cgtctacgcc
    16681 aaggaaagcg gctctgctgc agcaccgact gctggtcttc acttcaccaa agaactgctg
    16741 gcagaaatcc aagctaaggg tgttcatcta gtctatctga ctctccatgt cggactcgga
    16801 acctttagac ctgtttctgt ggataatctg gacgaacacg aaatgcactc agagttctat
    16861 caactttctg aggaagctgc tgccaccctt cgctctgtca aaaaaaatgg tggtcgtgtc
    16921 atcgctgtcg gaaccacttc tatccgcacc ttggaaacta ttggttccaa gtttgatggg
    16981 caaatccaag cagattctgg ttggaccaat atctttatca aacctgggta tgagtggaag
    17041 gtcgtggatg ccttctcaac caacttccac ctgccaaaat caactctggt catgttggtt
    17101 tctgcctttg caggccgtga attagtctta gatgcctacc accattccat ccaagaacac
    17161 taccgcttct tcagttttgg tgacgccatg tttatttatt gagaaagaat ttctctaaat
    17221 cttctaatac caataaatcg ctaagatatt atttcaaaga acatctacaa ttgaaactct
    17281 agctagctgt agaagacgcc tagtacattg aaattaaaat gcttccccct agcttcgaaa
    17341 atattgccat agattgcgtt gactctccaa attgattcat ctatatttta tttcagcttc
    17401 ctatattttc ttcgctgttt gtaaatcaaa atgcaagaca catgagtagc accatatttg
    17461 ttactcttat ctgtcctctc aagagactat tatgagttat ttcagaatca ttcactactt
    17521 tgaccctgac tctccttagt ctcaaaatca aagacttata ctcttcaaaa atctcttcaa
    17581 accgcgtcaa cgtcaccttg gattatatat gtgactgact tcgtcagttc tatctacaac
    17641 ctcaaagcag tactttgagc aacctgcgac tagttttcta gtttgctctt tgattttcat
    17701 tgagtattaa acaaaaagtg aacaaatctg aattctaatg tacagaagac taggcttgtt
    17761 cactttttta tagtcgctat aagatgacct tatctatagc tttttatata taattatata
    17821 ttcagacata ctattatcaa ttttgtcgca gggaggaatc tgttaacgca cccattcacc
    17881 attatcattg actctatagc catctatact tgtattgacc gctaactcac ccgatgcatt
    17941 tacataatac catttaccac caacttggaa ccattgattg actttcatag aaccgttgct
    18001 gttgaggtag taccatgaac cattaacttg tacccaacct gttgccatgg aaccatcagt
    18061 attataaaaa taccacatac cattttcttg tttccagtct gttgttggag caactgcttt
    18121 agctggttct actgctacat ctgttccttg gttagatgta acagatacag gatacgaagg
    18181 aatagatgat tgctcaggaa caacaacttt ttcaggttct ctcgtccctc tcattatacg
    18241 tctttttacc atctctttag taatttgacg agaagtagtt tcttcaattg ttccatcacg
    18301 ttcatctaca gtatagattg tagtaagagt aatttaccaa tttctcctac ttcttgactt
    18361 ttatcaagag ttgggccatc gagatattct gtttcgattg gaatttcttg gacaagaact
    18421 tggggcttgg ttcctttttt aacaactctt gtttgagagt cttctttttg acttaaagta
    18481 ctctcagtta cttgtccact ctttccatct acatcataag ttatcgttgt aactgttttc
    18541 ccattctttc ctagagtaat ctcttgctcc tgtcctgcag aaaggtcatt gtctgcttca
    18601 tatttagtag caaatggaac aagaacttct tcaaccttgc ttttagctgg aactttgata
    18661 actgtatccg tggcttcttt tctatcaaca gtaacctttt cggtaacatg accagtctct
    18721 ggattaacat cgtaggtcct tgtcgtagtt acatagccat cctctccatc aattgtaaca
    18781 ggattttcac tacggtcttt tgtttcatct ttttcataac gaattcgcgt acttgaaatt
    18841 ttcttggtta ctaccttagg tttagtcgct acttttacaa taatatcccc attgtcagcg
    18901 tcatcatact ctattccctc ttctttatct ctagtatcat ctctgacata ttgaatccca
    18961 tcagcagcat gaacaaaact tgtattcaga ttcctcctaa aaataaagtt agcccgatta
    19021 ccgcagaacc aaaaatcttt ccgagtttac gtattgcata gcgcttatta gtattagatt
    19081 ttgccattac atcctacttc tagtatagca tcttttctat caaacgttaa acaatatact
    19141 ttatatataa aatagactta gaatgatata ttgattattg aactaa
//
LOCUS       DAVGJJ010000032        18906 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000032 DAVGJJ010000000
VERSION     DAVGJJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.032"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(1..805)
                     /locus_tag="VVE35_001053"
                     /pseudo
     CDS             complement(1..805)
                     /locus_tag="VVE35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            870..1151
                     /locus_tag="VVE35_001054"
     CDS             870..1151
                     /locus_tag="VVE35_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU3061263.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDLVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            1283..2227
                     /gene="manA"
                     /locus_tag="VVE35_001055"
     CDS             1283..2227
                     /gene="manA"
                     /locus_tag="VVE35_001055"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU3061264.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(2267..3718)
                     /locus_tag="VVE35_001056"
     CDS             complement(2267..3718)
                     /locus_tag="VVE35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU3061265.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINMGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWIFLLRFVIPIIIIVVFIAQFM"
     gene            3823..4572
                     /locus_tag="VVE35_001057"
     CDS             3823..4572
                     /locus_tag="VVE35_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU3061266.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(4592..5047)
                     /locus_tag="VVE35_001058"
     CDS             complement(4592..5047)
                     /locus_tag="VVE35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3061267.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5067..6242)
                     /locus_tag="VVE35_001059"
     CDS             complement(5067..6242)
                     /locus_tag="VVE35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU3061268.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVNGGEVLSLDVLPQLSEKNLVTFLEKNREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(6301..7146)
                     /locus_tag="VVE35_001060"
                     /pseudo
     CDS             complement(6301..7146)
                     /locus_tag="VVE35_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
     gene            7271..7828
                     /locus_tag="VVE35_001061"
     CDS             7271..7828
                     /locus_tag="VVE35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU3061269.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7847..8314
                     /locus_tag="VVE35_001062"
     CDS             7847..8314
                     /locus_tag="VVE35_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU3061270.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMNDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8403..9032
                     /gene="upp"
                     /locus_tag="VVE35_001063"
     CDS             8403..9032
                     /gene="upp"
                     /locus_tag="VVE35_001063"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU3061271.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKVLQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9206..9796
                     /gene="clpP"
                     /locus_tag="VVE35_001064"
     CDS             9206..9796
                     /gene="clpP"
                     /locus_tag="VVE35_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU3061272.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9875..10123
                     /locus_tag="VVE35_001065"
     CDS             9875..10123
                     /locus_tag="VVE35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU3061273.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10224..11384
                     /locus_tag="VVE35_001066"
     CDS             10224..11384
                     /locus_tag="VVE35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3061274.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11652..12521
                     /locus_tag="VVE35_001067"
     CDS             11652..12521
                     /locus_tag="VVE35_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU3061275.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12525..13481
                     /locus_tag="VVE35_001068"
     CDS             12525..13481
                     /locus_tag="VVE35_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU3061276.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13481..14245
                     /locus_tag="VVE35_001069"
     CDS             13481..14245
                     /locus_tag="VVE35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061277.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            14245..14954
                     /locus_tag="VVE35_001070"
                     /pseudo
     CDS             14245..14954
                     /locus_tag="VVE35_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            15262..15918
                     /locus_tag="VVE35_001071"
     CDS             15262..15918
                     /locus_tag="VVE35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU3061278.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16026..17121
                     /gene="prfB"
                     /locus_tag="VVE35_001072"
     CDS             join(16026..16097,16099..17121)
                     /gene="prfB"
                     /locus_tag="VVE35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU3061279.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQGEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            17139..17831
                     /gene="ftsE"
                     /locus_tag="VVE35_001073"
     CDS             17139..17831
                     /gene="ftsE"
                     /locus_tag="VVE35_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU3061280.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17824..18750
                     /locus_tag="VVE35_001074"
     CDS             17824..18750
                     /locus_tag="VVE35_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061281.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5791 a         3574 c         4084 g         5457 t
ORIGIN      
        1 ctcagtatta catccaccag ctattaagaa aactgatgcg cctgtatggg catcaacagc
       61 tccataacaa tagcgaaatt ctcgtatata gtgactatgg atatgtggac ctactcctat
      121 tggagcccaa caagatccca gtttactgat tctaccgaaa ccagcctcat cttggtacat
      181 caagcgaacc ttacgaaaac gtctactggt tttaaagcgc tttcttgtct tcttgaattg
      241 agattttatt tttagacgcg acaatggttt gagcgtctgc tttcttagga tgttctggac
      301 gtggcataat atttcgccaa ccatggcgct tcaatagttg atagaaggcg tcacgtgtgt
      361 aggaacgacc taactccttt ttataagcct gaaataaggc atcaattgca acaaattctc
      421 ctgactctgc agccttcaaa tgacgggcaa gaaaggcttt ctcttcctca actgtcatat
      481 atgcatggtt acgaccacta cgtgtttctt gaaggagaga gtcgagtccg aactcctcat
      541 atttttttac atttcgctaa atcgttgttt gattacagtc taaaagttct ataatctctt
      601 tataagattt gcccatcaga cgaaatataa taaattgaaa ctagaatagt acacctctac
      661 ttctaaaata ttattagaaa tcgatttgtc ctgttcttgt ttcattttac tatagaacga
      721 tttgaaggcg tttatgatat ttagctgtac gagagtcttt taaaagtgtt ttgatggttt
      781 ggatttcttc tttagttgat ttcatatcac tgttatataa tgctttttga ttttagttta
      841 gtattatcgt agaaaatgga ctcggtgcta tggatacacc ggatgagaat ggttatgtag
      901 cagatgacta tcggattact tacttagagg cccacatcaa ggccatgcga gatgccattt
      961 acaaagacgg ggttgacttg cttggttata cgacatgggg ctgtatcgat ttggtttcag
     1021 ctggcacagg ggaaatgaat aagcgctatg gctttatcta tgtagatcga gataatgtag
     1081 gaaatgggac tctcaaacgt agcaagaaga agtccttcta ttggtacatg tcatttatag
     1141 caatggtgta agcatcgagt agagcacatt tgtttactat ttttataaaa tcttctccct
     1201 gctttataag atgtaaaaaa gagttcaatt cgaactcttt ttttgctata atgaggggag
     1261 aaaaatcaga caggagatcg acatgtcaga accattattt ttacaatcag ttatgcaaga
     1321 aaaaatctgg ggtggagcca agctacgtga tgagtttggc tacgacatcc caagtgaaaa
     1381 aatcggagaa tattgggcca tctcagccca tccaaatgga gtctctaaag ttgccaatgg
     1441 tcgttacgag ggaacagatc ttgctacttt gtatgcggaa caccgtgaat tatttggcaa
     1501 tcgtccagaa cctgtatttc cacttttgac caagatcctc gatgccaacg actggctcag
     1561 tgtccaagtt cacccagacg atgcttatgg actcgagcat gaaggcgaac tcggaaaaac
     1621 agaatgctgg tacattatcg cagcggatga aggttcagag attatctacg gtcacaatgc
     1681 caagtcaaaa gaagaactcc gccagcaaat cgaggacaag aactgggatg acttgttgac
     1741 caaagtccct gttaaggctg gagatttctt ctatgtacca agcgggacta tgcacgctat
     1801 cggggcgggt atcttaatcc ttgaaaccca gcagtctagc gataccacct atcgcgtcta
     1861 tgactttgac cgcaaggatg acaaaggcaa cttgcgtgaa cttcatcttg aaaaatccat
     1921 cgatgtcttg aacattggtg agccagcaaa tagccgtcct gtaactgtta aagcagatga
     1981 tttgcgttcc actctccttg tatctaatga tttcttcgca gtttacaagt gggaaattac
     2041 tggaaaagtt gactttgaaa agacagctga ctacagctta ttgagtgtct tagctggtca
     2101 aggtcagcta actgttgacg ggaaaaatta tccaattcaa aaaggcagcc actttatcct
     2161 accaagtgat gttgaagctt ggactctgga agggcaaggt ttggaattga ttgttagcca
     2221 tccataaaaa gaaagggcct gagttcacta ctcaagtcct ttttgattac ataaattggg
     2281 caatgaagac cacaatgatg atgattggaa tgacgaaacg aagaaggaag atccagactt
     2341 ggaacagtcc ttgtttccat gctctttcat cgagatggag ttcctccatt gcaagagcct
     2401 ttttaaagat atagcctgta aaaagtgaaa ggtagagagc tccaaatggc atgaggagat
     2461 tggaaaccaa gaagtccata gcgtcaaaga aggtcttacc aaaaatgtga acatccgcca
     2521 tgacaccgta agatagggct gaaggaatgc caaagacaaa ggtcaaaatt cctaaaataa
     2581 cactccattt ggcacgtttg ctgttatcct ggttggtgat attgcccata ttgatctcca
     2641 gcatcacgac agaagaagtg acagtcgcaa agaggaagag gacgtagaaa atggttccaa
     2701 aaggcatctt gtcaaagagt tgaggcaaga cgataaagag caagctcggt cccccttcag
     2761 actggatatt gaagggggat cgagcttgga aaatggctag acctgccatg atggatatcg
     2821 agatattcat ggctacgatg gagattcctg actggactag attggttttc ttgtccaagt
     2881 aagaagcata ggtcaacatg actgtaaccc ctagtgagag ggcaaagaaa gattgtccca
     2941 gagcatagag gagaccagcg ctagtcagtt ttgaaaagtc tggtttgagg aagtaaagaa
     3001 ccccttccat ggcatttggc aaactgagag agcgaccgat gataacaaca aagatgataa
     3061 agagcagggg catcatgact ttcgaagctc tttcaatccc tttttgaacc ccacgtgata
     3121 caataaagat atttaataag ataaaggctg cttgagctac tagggcaatg gctggatttg
     3181 aaatgattga agtaaataac tgagcataat cacccgttcc accaagttgg aacaatttcc
     3241 caaactcaat acctagatag actagaatcc atcctccgat aacactgtaa aaagataaga
     3301 ggataaagag ggcaaaggcg ccaatccaac cgataaagtt gtacttgtta ttcttgccca
     3361 gttttccaaa ggttttgata gcggaaacgc cagcactacg gccaagggca aactcagcca
     3421 gcaggagagg gaaaccgatt aaaatagtgg aaatgagaaa gattagtaaa aagcctccac
     3481 cgccattagc agcagtcatg tagggaaact tccaaacgga accaagcccg atgaccgagc
     3541 cagcagatgc tagaataaag ccaagtttcg acccccattg tgatttttct gacatagtta
     3601 aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa tagccttctg
     3661 tttgattgat agacttgact atcctatcat gttttctaag gtctgtcaag aaaatcattt
     3721 tcaagttttc acacctttct caaaaaagtt aaaaaatttt ctcaaaaacg cttgactctg
     3781 acctaagggg aggggttata ctatcattgt aaggaggaaa tcatgtacca tataaaagaa
     3841 gctgcgcagc tttcaggtgt ctctgtcaag accctgcatc actatgacaa gataggactc
     3901 ttggtcccct taaagtcgga aaacggctat cgaacctaca gtcaagagga tttggaacgc
     3961 cttcaggtca ttctttacta caaatatcta ggcttttctt tagagaaaat agcagagctg
     4021 ttaaaggaag aaaggacaga tttattgccc catttgacta ggcagttgga ctatctaact
     4081 cgcgaaaggc aacatctgga taccttgatt tccaccttgc aaaaaactat tcaagaacaa
     4141 aaaggagaaa gaaaaatgac cattgaggaa aaattcacgg gatttagcta tcaagacaat
     4201 caaaaatacc accaagaagc ggtagagaaa tatggtcaag aagtcatggg acaagcgctc
     4261 gaacgccaaa aaggtcacga agacgaggct acggccgcct ttaaccaagt ctttcaaact
     4321 ttggcacaaa atcttcaagt tggtttacct gcaacagcaa ccgaaaacca agagcaagca
     4381 gccaagctct tgcaagccat tcgcacttat ggatttgact gctctattga ggtattcggt
     4441 catatcggta aaggttacgt ctacaaccca gagtttaagg aaaacattga caagtttggt
     4501 tctggaacag cccagtacac gtcagatgcc attgcagctt acgttcagac aaatgcagaa
     4561 aaaataggct aggaatttcc tagcctattt tttacttcaa atcataaagc cagtcgtcac
     4621 cgtttttgta gtaaaagaat tcactgagat cttcttctag aaacacacga agcatatcag
     4681 acatatcatc ggttgcaagt tttagatgag aaagattttc aaagtcctcc caccaaactt
     4741 tcccttcgtc tgaagactgg agttcaccag taaagtgttc tgtcttgtaa aaaaggacga
     4801 cataacgata atccttgtcg tcataccagt ttttgatacc acagagttgg ggtttggaaa
     4861 tgatcagacc agtttcttct ttcacttcac gaatgacagc atcgacaaag gattcgccac
     4921 gttcaacatg accaccagga aaagtaatgc caggccagtc gggactaact cggtcttgga
     4981 ccaggacctt atctccgttt ttaatcatac acatgttaac aaattcgact gcctctcttc
     5041 tgttcattct tcacaacctt taatctttaa tcataatgca gacttcccgc cacccagccg
     5101 gtacagaggg cagaagtgat gttaaagcca cccgtgtggg cattgatatc cataacttcg
     5161 cctgcaaagt ggaggccagg taccagctta ctttcaaggg ttttaggatt gatttccttg
     5221 agactgactc cacccttggt aacaaaggac tttgcaaggg acatttttcc agttacagga
     5281 attttaagtt ctttaatgga ctggacaagc tgttctcgtt ccttttcagt cagctgtttg
     5341 actttttcag gatatccttg tacaaaaaat tcggccaagc gttctggtaa caaggttttt
     5401 aaagcgtttt tcaaggattt ttcccgattt ttttctagaa atgtaaccaa gttcttctca
     5461 gaaagttgag gcaaaacatc gagtgagaga acctccccac cgtttacaaa gctagacatg
     5521 cgtagggcag caggacctga caaaccaaag tgggtaaaga gtaaatcatg agtgatgaca
     5581 tgcttaccat aacttagggt cacatcgtcc agagaaatcc cttgtaaggc tttatgtgga
     5641 aaatctgtta ataaaggact ttcagcagcc tcaagatctg tgatggtatg cttaaaatgg
     5701 cgagcaatct cgtgaccaaa accagtcgaa ccagtcgaag gataagactt accacctgtt
     5761 gtgacaatga gtttctcaca agtgaaggtt tgatccgctg acttaaggac aaactggtca
     5821 tctacttttt taacagaaac gatttctatt tgagtagcaa cttgaccacc tagttcggtg
     5881 attttctttt ccaaagcttc gataatagtc cgagacttgt cactggctgg aaagacgcgt
     5941 ccgtggtctt cgaccttaag tttaacacca ttttctgtaa aaaagttgat gatgtcatga
     6001 ttatcgaact gggagaaaac actgtaaaga aagcgtccgt ttccaggaat tccagctagc
     6061 aggttgtcta agctaccatt gttggtcaca ttgcaacgtc ccccaccagt cccagctaat
     6121 ttttttccaa gtttccgatt tttttcgatg aggagggttt tctgtccata aaagctactg
     6181 gaaatcgtag ccatcatacc agcaggtccc ccaccgatga caatagtatc aaaatgtttc
     6241 atagctctat tgtaccacaa aaaaacaaga gatgatggtc acctcttgtc aagaatgcaa
     6301 ttaatcaatt tcatagccca tcagcaaacc gccctcttct gcatagaaac tgcagagacc
     6361 agaggttggt agaattttaa tatccgcttg tgggaaggtt tcacggattc gctctgagag
     6421 ctgttgacaa catttttcgt tattgcgttg ggccatgaca atacggccac cagcatatcc
     6481 agcttttact aactcatcat aggcagcttg aactgatttc tttgatcccc ttgctttttg
     6541 tagcaattcg agagttccag tttcactagc ttttccgacc atacgaatgt tgagaaggcc
     6601 aacgaccgta ccgataagct tgctcaaacg gccgttcttc accaagttat cgactttggc
     6661 taggacaaag agcaacttag ttttttctta ataggcagtg atagcttcaa ccacttcttc
     6721 aaaagacaag ccctggtcaa tcaagtcatt caatttttct acgagtaggt caacttcacc
     6781 accagcagat aaactatcaa tcacatgaat cttagtgtca ggatggtctt ccagataaat
     6841 attctttgct agttgagcac tattgtgact gccagaaagg gtacctgtga tggttactag
     6901 gaaaatgttt ttggcacctt caaatgctcg caaatagtca tctgggcttg gacaagccga
     6961 ttttgaagct tctgcagttg catacatggt ttccatcatt tggtcaatat cgagactggc
     7021 gtcatcaaca aagacctgat cagctacttg aatggttaag gggacactta caaaggttgt
     7081 gttaatagct ggtgttggca gttgacgata atcacaacca gagtcagcaa taatcttcca
     7141 agtcatagaa attctccatc tttgtcagtt atacattgac aaaggttctg tcttttttta
     7201 caattatatc atgaaaaccc ttgaaacaaa agcatcatcc gtctgttgtg acaagtagaa
     7261 agaaaatgtt atgtctgaac gtagaatctc tgaaaagtct cttgaaaatc tcagaaaatc
     7321 aaaccaagaa tccaatttat taactagaga agccattgaa acagccctct tgcaactctt
     7381 ggaaaaaaag gaactgacca agattagtat ttctgaattg gtcaaacgtg caggtgtttc
     7441 gcgtgcggcc ttttatcgca attatgactc caaagaggaa attttagaga gcgtctttaa
     7501 acgaactgtc cacaatatta tggaacagct gcatcattac gatttaaaga cagaccttta
     7561 tttggtttgg gttcaccttt tccgggaggc cagaaaggaa gctagagtaa ttcaattggc
     7621 cttggattac catctggaaa aaatctttgt ccaagccatg caggaatttc tagaaaaata
     7681 ccatgggaaa tcaaaaggtg tcagctctta tcttcattcc ttctggagct cagccatcgt
     7741 ctctgtcctt ctaaaatgga tcaaggatgg catgaaggta ccagctgaaa agattgcaga
     7801 tttaggttta ccatttttta aaaaatagag aaaaaggaga aaagagatga ctgaaaaaag
     7861 actggcatgg gatgagtatt ttgcagccca agccctacta attgcgaatc gttccacttg
     7921 taaacgtgct aaagtgggcg cgattctggt aaaagataat aaggttattt ccactggtta
     7981 caatggttcg gtgtcaggga ctgagcattg tattgatcac gaatgtttgg tcattgaagg
     8041 ccactgtgtt cgcacccttc acgctgaggt caatgctatc cttcaaggtg ctgagcgtgg
     8101 tgttcccaaa ggatttacag cctatgtaac ccattttcct tgtctcaact gtacaaaaca
     8161 attgcttcag gtcggctgca agcgcgtggt ttatatcaac cagtaccgaa tgaacgacta
     8221 cgcccaatac ctttatcaag aaaaagggac agaattgacc catttaccac ttgagacagt
     8281 acagacagct cttaaagagg ctgatctaat gtaaaaatta tcaaaaataa atggtttaga
     8341 aagattttta aaccgtattt tggtataata agaagaataa attgaaagaa ggaattccaa
     8401 aaatgggaaa aattgaagtt attaatcacc cactgattca acacaaattg tcaattttgc
     8461 gtcgtacaga tacttctaca aaagcttttc gtgagctagt agatgagatt gcaatgttga
     8521 tggggtatga agtacttcgt gatcttccac tagaagatgt ggaaatcgaa acaccaatta
     8581 caaaaacagt tcaaaaacaa ttggcaggta agaaattggc catcgtccca atcttgcgtg
     8641 caggtatcgg gatggttgat ggtctcttga gcttggttcc agctgctaaa gttggccaca
     8701 tcggtatgta ccgtgatgaa gaaacacttc aaccagttga atacttggtg aaattgcctg
     8761 aggacattga ccaacgtcaa atttttgtag tagacccaat gttggcaaca ggtggctcag
     8821 caatcttggc tgttgattct cttaaaaaac gtggcgcatc aaatatcaaa tttgtctgcc
     8881 ttgtatctgc tccagagggt gtaaaagtcc ttcaagaagc tcatccagat gtagaaatct
     8941 ttacagcagc cttggatgaa cgtttgaacg aacacggtta tatcgttcca ggtcttggag
     9001 atgctggaga ccgcttgttc ggtacaaaat aagatcgaaa agaagaatga cttggaaaag
     9061 tatttctagt cacgaaagga ggttgggttt ttgtttctgt ctaatgaaag cagagcaaaa
     9121 atttgacctt ttttgaccaa gatattataa tagtcttatc ttcagtcatt tgaccaacaa
     9181 aattaaaact caaaaggaga aatgaatgat tcctgtagtt attgaacaaa caagccgtgg
     9241 agaacgttct tacgatattt actcacgcct tctcaaagac cgcatcatta tgctgacagg
     9301 tccggttgaa gacaatatgg ctaactctgt tattgcccaa ttgcttttct tggatgccca
     9361 agatagtacg aaagatattt acctttatgt caatacacca ggtggttctg tttcagctgg
     9421 tttggcaatc gtagatacca tgaactttat caaggcagat gtccaaacca ttgttatggg
     9481 aatggctgca tctatgggga ctgtcatcgc atcaagtgga gcaaaaggca aacgtttcat
     9541 gcttccaaat gctgaataca tgattcacca accaatgggc ggtacaggtg gtggtaccca
     9601 acaaactgat atggctatcg ctgcagaaca cttgctcaaa actcgtaata ccttggaaaa
     9661 aatcttggct gaaaattcag gtcagtcaat ggaaaaagtc catgcagatg cagaacgtga
     9721 taactggatg agcgcccagg aaacacttga atatggcttt attgatgaaa ttatggccaa
     9781 caattcattg aactaatgat gatagaaggc aaactcgact gggtttgctt tttttggtat
     9841 aatagggaga gatttcttag aaagaggatt tatcatgttt gaaaaagtca atcgctctgg
     9901 cttgattatc tatctttact ataatcgtga tgccaaaaaa ctgcaggatt atggagatat
     9961 tacctatcat tccaagaaac atcgttactt acaactctat gttccaactc aagaagtgga
    10021 gcaattggtc ggacgcttga gcaaggaaaa atttattaaa aaagttaggg tttgtcatat
    10081 ccaagagttg gaaacaccct ttgtgggcaa tctttatcga taggaaaacg ttatcatcga
    10141 aaaagttcaa ggaaaacatt gacaattttc tgataattcg gtatattctt aacagactat
    10201 ttaagaaata aggagacaaa aagatgaaga aaaaatttgc cctatcgttt gtggcgcttg
    10261 caagtgtagc acttcttgca gcctgtggag aagtgaagtc tggagcagtc aacactgctg
    10321 gtaactcagt agaggaaaag acaattaaaa tcgggtttaa ctttgaagaa tcaggttctt
    10381 tagctgcata cggaacagct gaacaaaaag gtgcccaatt ggctgttgat gaaatcaatg
    10441 ccgcaggtgg tatcgatgga aaacaaatcg aagtaatcga taaagataat aagtctgaaa
    10501 cagctgaggc tgcttcagtt acaactaacc ttgtaaccca atctaaagta tcagcagtcg
    10561 taggacctgc gacatctggt gcgactgcag ctgcggtagc gaacgctaca aaagcaggtg
    10621 ttccattgat ctcaccaagt gcgactcaag atggattgac taaaggtcaa gattacctct
    10681 ttattggaac tttccaagat agcttccaag gaaaaattat ctcaaactat gtttctgaaa
    10741 aattaaatgc taagaaagtt gttctttaca ctgacaatgc cagtgactat gctaaaggga
    10801 ttgccaaatc tttccgcgag tcatacaagg gtgaaatcgt tgcagatgaa actttcgtag
    10861 caggtgacac agacttccaa gcagccctta caaaaatgaa agggaaagac tttgatgcta
    10921 tcgttgttcc tggttactat aatgaggctg gtaagattgt aaaccaagcg cgtggcatgg
    10981 gaattgacaa accaatcgtt ggtggtgatg gattcaacgg tgaggagttt gtacaacaag
    11041 caactgctga aaaagcatca aacatctact ttatctcagg cttctcaact actgtagaag
    11101 tttcagctaa agctaaagcc ttccttgacg cttaccgtgc taagtacaat gaagagcctt
    11161 caacatttgc agccttggct tatgattcag ttcaccttgt agcaaacgca gcaaaaggtg
    11221 ctaaaaattc aggtgaaatc aaggataacc ttgctaaaac aaaagatttt gaaggtgtaa
    11281 ctggtcaaac aagcttcgat gcagaccaca acacagtcaa aactgcttac atgatgacca
    11341 tgaacaatgg taaagttgaa gcagcagaag ttgtaaaacc ataatagaaa aatgttgaaa
    11401 tagggaatga gcctttgact cactccctgt ttcgatattt aatactcttc gaaaatctct
    11461 tcaaactgcg tcaacgtcgc cttggattat atatgtgact gacttcgtca gtcttatcta
    11521 caacctcaaa gcagtgcttt gagcaacctg cggctagttt cctagtttgc tctttgattt
    11581 tcattgagta taagaaccta tcaaaaagtg agggaaaccc ctcggaatta taaatagaaa
    11641 gagtgaatct tatgctccaa caactcgtaa atggtttgat tctaggtagt gtttacgcgc
    11701 tgttagccct aggatatacc atggtttacg gaattatcaa gctcatcaac ttcgcccatg
    11761 gtgatattta tatgatggga gcctttatcg gttatttctt gatcaattct ttccaaatga
    11821 atttctttgt agcgcttatt gtagctatgc tagcgacagc tattcttggt gtcgtgattg
    11881 agtttcttgc ttaccgacct ttgcgccact ctactcgtat tgctgttttg attacggcta
    11941 ttggggtttc tttcctattg gagtatggaa tggtctatct ggttggtgcc aatacccgtg
    12001 ccttccctca agcgattcaa acagttcgat atgatttggg accaattagc ttaacaaatg
    12061 tgcagttaat gattttgggc atttccttga ttttgatgat tttgttacaa gtcattgtcc
    12121 aaaagactaa gatggggaaa gccatgcgtg cagtatcagt agatagcgac gcggcgcaat
    12181 tgatggggat caatgtaaac cgtacgatta gctttacctt cgctttgggt tctgctcttg
    12241 cgggtgcggc tggtgttctg attgctcttt attataactc tcttgagcct ttgatggggg
    12301 ttactccagg tcttaaatct ttcgttgccg cagtacttgg tggtatcgga attattcctg
    12361 gtgctgctct tggtggcttt gtgattggtc tattggaaac ctttgcgaca gcctttggga
    12421 tgtcagattt ccgtgatgcc attgtttatg gaatcttgtt gttgatcttg attgtccgcc
    12481 cagctggtat ccttggtaag aatgtgaaag agaaggtgta aacgatgaag gaaaatttaa
    12541 aagttaatat tctatggtta ctccttttgt tagctggcta tagcttgatt agtgtactgg
    12601 tttcagtcgg agtacttaat ctattctatg tacagatttt acaacaaatt ggaattaata
    12661 ttattttggc tgttggtctc aacttaatcg ttggtttttc aggacaattt tcacttggtc
    12721 atgctggttt catggcgatt ggtgcctatg cagcagctat tattggttct aaatcaccaa
    12781 cctacggtgc cttctttgga gctatgcttg taggggcttt gctttcagga gcagttgcct
    12841 tacttgtcgg cattccaacc ttgcgcttga agggggacta tcttgcggta gcaactctag
    12901 gtgtttctga aattatccgt atctttatca tcaatggtgg aagccttaca aatggtgcgg
    12961 caggtatctt agggattcct aactttacaa cttggcaaat ggtttacttc tttgtcgtga
    13021 ttacaaccat tgcaaccttg aacttcttgc gtagcccaat tggtcgttca accctctctg
    13081 ttcgtgaaga tgaaatcgct gctgagtcag ttggggttaa tacgactaaa attaaaatca
    13141 tcgcttttgt ctttggtgcc attactgcaa gtattgctgg gtcacttcag gcaggattta
    13201 tcgggtctgt tgtaccgaaa gattacacct tcatcaactc aatcaacgtt ttgattattg
    13261 ttgtatttgg tggactcggt tccattacag gtgcgattgt ttcggctatt gttctgggaa
    13321 ttttgaatat gcttctccaa gatgttgcta gtgtgcgtat gattatttac gctttggcct
    13381 tggtattggt aatgattttc agaccaggtg gactccttgg aacatgggaa ttgagcctat
    13441 cacgtttctt taaaaaatct aagaaggagg aacaaaacta atggcattac ttgaagtaaa
    13501 acagttaacc aaacattttg gtggtctaac agctgttgga gatgtgactc ttgaattgaa
    13561 cgaaggggaa ctggttggat taatcggtcc aaacggagct gggaaaacca cccttttcaa
    13621 ccttttgacc ggtgtttatg aaccaagtga gggaacagta accctagatg gtcacctttt
    13681 gaatgggaaa tcaccttata agattgcctc tttgggactt ggacgtactt tccaaaatat
    13741 ccgtcttttt aaagatttaa cagttttaga caatgttttg attgcttttg gaaaccatca
    13801 caaacagcat gtttttacta gtttcttacg cttaccagct ttttacaaga gtgaaaaaga
    13861 attaaaggct aaagctttgg aattgttgaa aatctttgat ttagatggtg atgcagagac
    13921 tcttgctaaa aatctttcct acggacaaca acgtcgtttg gaaattgttc gtgcccttgc
    13981 tacggaacct aaaattctct tcttagatga accagcagca ggtatgaacc cacaggaaac
    14041 agccgaattg actgagttaa ttcgtcgtat caaagatgag tttaagatta caatcatgtt
    14101 gattgaacac gatatgaatc tggtcatgga agtaacagaa cgtatctacg tacttgaata
    14161 tggccgttta atcgctcaag gaactccaga cgaaattaag accaataaac gcgttatcga
    14221 agcttatcta ggaggtgaag cctaatgtct atgttaaaag ttgaaaatct ttctgtgcat
    14281 tacggtatga tccaagcagt tcgtgatgta agctttgaag ttaatgaagg agaagttgtt
    14341 tcccttatcg gtgccaacgg tgcaggtaag acaactattc ttcgcacctt gtcaggtttg
    14401 gttcgaccaa gttcaggaaa gattgaattt ttaggtcaag aaatccaaaa aatgccagct
    14461 cagaaaatcg tggcaagtgg tctttcacaa gttccagaag gacgccacgt ctttcctggc
    14521 ttgactgtta tggaaaatct tgaaatggga gctttcttaa agaaaaatcg tgaagaaaat
    14581 caagctaact tgaagaaggt tttctcacgc tttcctcgtc ttgaagaacg gaagaaccaa
    14641 gatgcagcca ctctttcagg ggggaacaac aaatgcttgc catgggacgc gccctcatgt
    14701 caacaccaaa acttcttctt ttagatgaac catcaatggg acttgcccca atctttatcc
    14761 aagaaatttt tgatatcatt caagatattc agaagcaagg aacaacggtc ctcttgattg
    14821 aacaaaatgc caataaagca cttgcaatct ctgaccgagg atatgtactg gaaacaggga
    14881 aaatcgtcct atcaggaaca ggaaaagaac tcgcttcatc agaagaagtc agaaaagcat
    14941 atctaggtgg ctaaaacaat ccagtggatt gttttagtcg gcagatggag attacgaagt
    15001 aatcatcaat atagtccggg ggaccttttt agtcggtaga ttgagattgc aaacaaatct
    15061 gcatctacat tgaaagctta atttctaata attgaaaaaa tcgaatgaaa aatttcttac
    15121 cttcattcac agagctcgat ttcagagctc tttttgctag cttattcata cttttctgaa
    15181 tttcgaaaaa gaaatgtaag cgtttgatag atttacaaaa agattgtata atagggataa
    15241 gaatagaaaa ggagaagtct catggcagtt aaagatttta tgacccgcaa ggtagtttat
    15301 attagtccag atacaacagt atctcatgca gcagatttga tgagagagca aggtttgcac
    15361 cgtctgcctg ttatcgaaaa tgatcaatta gttggtttgg tgactgaggg aaccattgca
    15421 caagcaagtc catctaaagc aacaagtctt tctatctatg agatgaatta tcttctgaat
    15481 aagacaaaag taaaagatgt catgattcgc gatgttgtca ctgtctcagg ctatgctagt
    15541 ctagaagatg caacttatct gatgttgaaa aataagatta gtattctccc tgtcgtagat
    15601 aaccatcaag tatacggagt tattactgac cgtgacgttt tccaagcctt tcttgaaatt
    15661 gcaggttatg gcgaagaagg gattcgtgta cgctttgtta cagaagatga agttggtgtt
    15721 cttggaaaaa ttgtttcttt gattgtagaa gaaaatttga atatctccca tacagtcaat
    15781 attccgcgta aggatggtaa ggtgattatc gaagtgcaaa tcgatggatc aattgattta
    15841 ccagccttga aagaaaaatt tgaagcaaat ggtattcaag tggaagaaat cgctcgcact
    15901 tcagcaaaag tcttgtaaga agggaagccc aaaggcttct tttttcatga aaaggggatt
    15961 agagaaaaag atggaaagaa atgataaaat atgctataat gaaataatgt aaaaaaggag
    16021 tatttatgga catttcagta attcgtcaga aaattgacgc aaatcgtgaa aaattagctt
    16081 ctttcagggg gtctctttga cctcgaaggg ctagaggaag agattgccat cttggaaaac
    16141 aagatgacag aacctgattt ttggaacgat aatattgcgg cccaaaaaac gtcgcaagaa
    16201 ttgaatgaat taaaaaacac ttacaatacc ttccataaga tggaagagtt gcagggtgaa
    16261 gtcgaaattt tattggattt tttggctgaa gacgagtcag tgcatgatga actggtagcg
    16321 cagttagccg aacttgataa gataatgacc agctacgaga tgactctact cttgtcagaa
    16381 ccttatgacc acaacaatgc catcttggaa atccatccag gttctggtgg tactgaggcg
    16441 caggactggg gtgatatgtt gcttcgtatg tatactcgtt atggtaatgc taaaggcttt
    16501 aaagtggaag tgttggatta ccaagcaggt gatgaggctg gtattaagtc ggtaacttta
    16561 tcatttgaag ggcctaatgc ctatggtctc ctcaagtcag aaatgggtgt tcaccgctta
    16621 gtgcgaatct caccatttga ctctgccaaa cgtcgccata cctctttcac atctgtagaa
    16681 gtgatgccag aattggatga tactattgaa gtggaaatcc gtgaagatga tatcaagatg
    16741 gataccttcc gttcaggtgg tgccggtgga caaaacgtca ataaggtttc aacaggtgta
    16801 cgtttaaccc acattccaac tggaattgtt gtccaatcaa cagtggatcg tacccagtat
    16861 ggaaatagag atcgtgccat gaagatgttg caggctaagc tctatcaaat ggagcaagat
    16921 aagaaggctg cggaggtaga ttctctcaaa ggtgagaaaa aggagatcac ttggggaagc
    16981 caaatccgtt cttatgtctt cacgccttat actatggtaa aagatcaccg aactagcttt
    17041 gaggttgctc aggtagataa ggttatggat ggggacctag atggttttat cgatgcttat
    17101 ctcaagtggc gaattagcta agatagaaag gaactcacat gtcaattatt gaaatgagag
    17161 atgtcgttaa aaaatacgac aacggaacga ctgctctacg cggtgtttcg gtcagcgttc
    17221 aaccggggga atttgcttac atcgtaggac cttcaggagc agggaagtca acttttattc
    17281 gttctctgta tcgtgaagta aaaatcgata aaggaagtct atcagttgct ggttttaatc
    17341 tggttaagat caaaaagaaa gatgtcccgc ttctacgtcg tagtgttggg gttgtcttcc
    17401 aagattataa attgttacca aagaaaactg tctatgaaaa tattgcttac gctatggaag
    17461 taatcgggga aaatcgccgt aatatcaaaa gacgagtgat ggaagttttg gacttggttg
    17521 gattgaagca taaggttcgt tctttcccaa atgaactctc aggtggggag caacagcgaa
    17581 ttgcgattgc gcgtgcaatt gtaaataatc ccaaagtatt gatagctgat gagccaacag
    17641 gaaatctgga tccggataat tcatgggaaa ttatgaatct cttggaacgg attaacctac
    17701 aagggacaac tattttgatg gcgactcata atagccagat tgtaaatacc ttgcgccacc
    17761 gtgtcattgc cattgaaaat ggccgtgtcg ttcgtgacga atcaaaagga gagtatggat
    17821 acgatgatta gtagattttt tcgccattta tttgaagcct taaaaagttt gaaacgaaat
    17881 ggttggatga cagtagctgc tgtcagttca gtcatgatta ctttgacctt ggtggcaata
    17941 tttgcatctg ttattttcaa tacagcgaaa ctagctacag atattgaaaa taatgtccgt
    18001 gtagtagttt atatccgaaa ggatgtggaa gataatagtc agacaattga aaaagaaggt
    18061 caaactgtta caaataatga ctaccacaag gtatatgatt ctttgaagaa catgtctacg
    18121 gttaaaagtg ttaccttttc aagtaaagaa gaacaatatg aaaaattaac cgagataatg
    18181 ggagataact ggaaaatctt tgaaggagat gccaatcctc tctatgatgc ctatattgta
    18241 gaggcaaaca ctccaaatga tgtaaaaact atagccgaag atgctaaaaa aattgaaggt
    18301 gtctctgagg ttcaagatgg cggtgccaat acagaaagac tcttcaagtt agcttcattt
    18361 atccgtgttt ggggactagg gattgctgct ttgttaattt ttatcgcagt tttcttgatt
    18421 tcaaatacca ttcgtattac cattatttcc cgcagtcgcg aaattcaaat catgcgcttg
    18481 gtcggagcta aaaacagtta tatccgtgga ccgttcttgt tagaaggagc ctttatcggt
    18541 ttattgggag ctatcgcacc atctgttttg gtctttattg tttatcaaat tgtttaccaa
    18601 tctgtcaaca aatcgttggt agggcaaaat ctatccatga ttagtccaga tttatttagt
    18661 ccgttgatga ttgccctact atttgtgatt ggggttttca ttggttcatt gggatcagga
    18721 atatccatgc gccgattctt gaagatttag gtaaaatagc tgcttttatg aggagattgt
    18781 aaaatctcct tttttgctac aagagttttt gaaaagagat gcgcagaaga aaagagcttc
    18841 caaagaagtc ctccagagaa gacttcttga tggctaattt gaacttgaaa tttggcgcac
    18901 tagaaa
//
LOCUS       DAVGJJ010000033        18840 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000033 DAVGJJ010000000
VERSION     DAVGJJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.033"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(183..554)
                     /locus_tag="VVE35_001075"
     CDS             complement(183..554)
                     /locus_tag="VVE35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU3061282.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDSQLRIG"
     gene            complement(700..2052)
                     /locus_tag="VVE35_001076"
     CDS             complement(700..2052)
                     /locus_tag="VVE35_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU3061283.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDDDLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLISEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILASNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKVDVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVILQKDMPVEVQIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(2049..2516)
                     /locus_tag="VVE35_001077"
     CDS             complement(2049..2516)
                     /locus_tag="VVE35_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU3061284.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2509..4728)
                     /locus_tag="VVE35_001078"
     CDS             complement(2509..4728)
                     /locus_tag="VVE35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU3061285.1"
                     /translation="MLKKYPCTIQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGTLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            5194..6057
                     /locus_tag="VVE35_001079"
     CDS             5194..6057
                     /locus_tag="VVE35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061286.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(6346..7665)
                     /gene="argH"
                     /locus_tag="VVE35_001080"
                     /pseudo
     CDS             complement(6346..7665)
                     /gene="argH"
                     /locus_tag="VVE35_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(7645..8764)
                     /locus_tag="VVE35_001081"
                     /pseudo
     CDS             complement(7645..8764)
                     /locus_tag="VVE35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(8781..9587)
                     /locus_tag="VVE35_001082"
     CDS             complement(8781..9587)
                     /locus_tag="VVE35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU3061287.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(9794..10435)
                     /locus_tag="VVE35_001083"
     CDS             complement(9794..10435)
                     /locus_tag="VVE35_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061288.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(10432..12540)
                     /locus_tag="VVE35_001084"
     CDS             complement(10432..12540)
                     /locus_tag="VVE35_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3061289.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(12595..12888)
                     /locus_tag="VVE35_001085"
     CDS             complement(12595..12888)
                     /locus_tag="VVE35_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU3061290.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            13295..13615
                     /locus_tag="VVE35_001086"
     CDS             13295..13615
                     /locus_tag="VVE35_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU3061291.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLVICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(13667..14239)
                     /locus_tag="VVE35_001087"
     CDS             complement(13667..14239)
                     /locus_tag="VVE35_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU3061292.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            14481..15152
                     /gene="sdaAB"
                     /locus_tag="VVE35_001088"
     CDS             14481..15152
                     /gene="sdaAB"
                     /locus_tag="VVE35_001088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU3061293.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            15161..16033
                     /gene="sdaAA"
                     /locus_tag="VVE35_001089"
     CDS             15161..16033
                     /gene="sdaAA"
                     /locus_tag="VVE35_001089"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU3061294.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            16055..16687
                     /locus_tag="VVE35_001090"
     CDS             16055..16687
                     /locus_tag="VVE35_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3061295.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            16737..16814
                     /locus_tag="VVE35_001091"
                     /pseudo
     CDS             16737..16814
                     /locus_tag="VVE35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(16990..18840)
                     /locus_tag="VVE35_001092"
     CDS             complement(16990..18840)
                     /locus_tag="VVE35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU3061296.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
BASE COUNT         5844 a         4047 c         3148 g         5801 t
ORIGIN      
        1 aatttgctcc actgattgct atcaatattt caattggtaa aaaatagtaa ttccaaataa
       61 aatgagaaat cagcaatgat agaaaaataa caatatcaat tttcctgttg ttgacatact
      121 ctgtaatttt atggtacagg aatgcaacaa taactgaaat taaaacaacc aacataagat
      181 tattaaccta ttctcaattg actatcacgt cgcccatccc agccatcaat aatattggta
      241 aaaatccaat cagctccagc accaattcca gcagatgcaa tacttctaat aacagtattt
      301 gcaaaagcat ttccaatatg aattcctaat actcctaaac ctttaacaat cccagctacc
      361 agtgcggagc gtaatccata agatgcaaga actagttgaa ggcctccacc tcctgctgct
      421 gctaatgctg catatgctgc accagtaatg gctcctgtaa ttgcccacct gggtatatat
      481 atagcccccc catcaaggta catcatctct tcttcatcaa tcacaacata attatttggt
      541 aatacgagtt ccatttattt tctccttttt tattaagcat ggttaggata gtttcctaac
      601 aagtgagcat cgactttgtt tatagcactt agttacctag ataagatatc ttccctttat
      661 cttactttat tttataggtg acttaatgtt taaaaccaat tattgcttga aacttaacat
      721 ttccaaaatc caatcaagat aggtttcttt atcatagaca atctgcactt caactggcat
      781 atccttttgg agtatgaccg tctcgcttcc atgttttaga gttaaggttt ctaattctat
      841 catgacttta tagaggctaa tattcccctc tttcgtttct tgggaaattg ttcctgaatc
      901 aatctgtctg acttgtccct ttagtgttcc atatttttga ctatttacac cagtaatagc
      961 aacatcaact tttgctcctt ttgagacacg agaaatatca ctcgcaagta caaaagcctc
     1021 tacatagtaa cctttttctt tccctgaaac ttctgctatc gtttgcccct gctgaataga
     1081 cattccttgt ttcaaaggta ccagataatg cagagtacct tcatttgacg ctaatatact
     1141 atgagcttta tcttgacctg ttgccactcc aagattagcc tcaagctctg taatactttt
     1201 attgttgttt gaacgagcag tacctaactc actaattaat tgagcataaa tattataagc
     1261 ctgagaagtt ggatcattat aggtacggtt catatcctta atattgcttt gttgactatc
     1321 aatctcgcta ttcttagaag taatatttgt ttttaatgct tcgatttttg ttgttatttc
     1381 tgatatctta gtttctaact tagctttgtt atcagctgat gtcgtatctg atagagaagg
     1441 cgagtctcct tgctgttgaa gttgaagcaa ttcattttgt agagcttgca agtcgtcatc
     1501 taactgattt ctttcagctg ttattttatt caacttcgta tattcatcct gaatctttga
     1561 atactgggta ccattattat aattttccga gtttaactga gacagatagt attcaacttt
     1621 cccataatat tcctgttctt caccagaatt agataaatga ttgtatttct catttaatga
     1681 ttgttcaaat ttttgaaaga tagcttcttt tgcacgtaac ttctccaatt gtttatttaa
     1741 agagctcgct tgtaaacctt cttgtccact tgataacgaa ataatgacat ctccagcatt
     1801 taccatatct ccttctgact tatgtaaagt aactaccttc cctgaaccaa ttgctgatag
     1861 gaactctgta cctgttataa ctgaatttcc attcgctttt acaatatagt ttttgggtat
     1921 ataagctgcg ccaaccaatg caccgcttaa gataatagca gttgaaataa tgagaataaa
     1981 cgcaaaagct ggtggtctct tatcaaagaa aatacgagaa taacgtaatt ctgatttatt
     2041 atataatttc ataggcttac aattggtcta aaaatatcta ctaccatttt ttcaggagaa
     2101 gaattaacat aaactgtata gacaatccca tccgtttgaa tatcattttc atagacatat
     2161 agatccaatt tagaatacgc atactgtaga tactctggac tgtcttcaaa acgaacatat
     2221 aaacaatatg gaacagagat agaatcctgt acatcataaa tgttactgta ctgttgagca
     2281 ttatgagctt gaatataaaa ctcaaaatca gtcgttatta atccatcatc atgaatagta
     2341 gtaccacaac tttttacaat taatggacca aaaatttgtg cttttaacaa ctgcaaatgt
     2401 tgatgaaatt tattaatttc ctgatctaca tcttctactt ttgtatcatg taacttttta
     2461 cagataactg actttagtac cagtttttta ttatctttta cctctaactt agccataagt
     2521 aacctcctct gtatctaaca cagcctgtga ctgaatttgt tgattcactt gaacgctctg
     2581 caaaccaacc attctagcat actttccatt tttcgccatt agttcttcat gagtcccata
     2641 ttccacaata gtcccatttt ctaaaaagca tatcttatca catcgaagaa ttgtagacag
     2701 cctgtgggct actacaattg ttgtcttatc cattatttta ttaaagatta aatcctgaat
     2761 aatctgttca ctaaatgaat ctaagttaga ggttgcctca tcaaatatat acaaatcagc
     2821 tttactcagt agtgctcttg caatagccaa tcgttgtttc tctccacccg agaaattttg
     2881 accgccttct tctacaaagg agccataacg attttgaagg cgttgaatcg tatcatgaat
     2941 accaacaata cgacaaactc tcaccatatc ctcatatgta acagaaggat taccaatttt
     3001 tagattatca ataattgtcc ctgtaaagag ttctatggtc tgaggaatgt aggaaatttt
     3061 cttacgtagt ttaccatagt caagatctga taagtcattt tctcctatac gaattttccc
     3121 ctcactcgga tttataaatc ttaataacaa acgaactagc gtactttttc ctgctccgct
     3181 ttctccaaca aaagctattt tttctccttg tttaattcgc aaattaaagt ccttgataac
     3241 aggagggcgt gaaccatagg caaatgttac atctttaaat tcaatgtcat cacataaggt
     3301 gaagtctcta atactataat ccaaggcaat gtcttcacga tctacttcca tcaattcttg
     3361 aagtcgacta accgcaacct gtacctcttg aaaggttaac tgcaaaccaa ctaaattctg
     3421 aataggttct gtaaaatatt gacttaatgt ctgaaaaacc aagaggtccc caatagtcat
     3481 ttttccatca atgataaata aggcgcccac tcccataaat aataacccac ccatggttga
     3541 ggtcattgaa gatattgtag attgaatatt ctgcaaaaca ccctctttat aaccaatttc
     3601 taaagtatga acaaattttt cttcaatttt gtttaaacgt tgttcctcgt catgctgaga
     3661 cttaatggta tcaatgttac gaatagattc aattaactgt gaattaagaa gtccatttgc
     3721 ttccatttgt tcatgattaa tttttttata aggcttttta aaacagtata taagaatgat
     3781 gttaacaata accataaaaa ctaatatcag aaataaagat tgattaatag tccaaaggac
     3841 aaccgcacta ataactgaca aggtgatatc catgactaat gaaatagaga ctgacgtaaa
     3901 cacattcttt atcgtcatcg catcttgaaa tctcgttaaa acatcaccaa ctctacgact
     3961 tccaaaaaaa ctataaggaa gatgaataat atggtcataa tatcccatta aaaccggtat
     4021 atcaattttt cgagatagaa acaaaagaac gtgctgacga aatgcagata gaagagtctg
     4081 taagaacgac actattccaa atactatcaa aaacatatac agactatttt ttaaagcata
     4141 tggaatcacc tcgtccatga taacttttga aaagagactc gataaaatac caatgatact
     4201 taaaacaaag gaagctaata tgactgtact aatcaatcct ttttgattaa aaattaacgt
     4261 tccaaataaa tcaagaagtc cttttccctt tagtttccct ctttcaaaat ccgaatttgg
     4321 aaccataaaa agagtaatac cttgataaat ttctcccagt tcgtcacttg acatcttgcg
     4381 tatgccacta gcagggtctg ctacgtaaaa tttactattc cttttgatac tatgtaatac
     4441 cacaaaatga ttttgaccta aatcattctt aacttgaaga atcgcaggaa atgttaattt
     4501 tggagtcaaa tcttccagtg ctactcgaac agcttttact gtaaaattca acttattcag
     4561 acctgaaaca ataccactga cagtcgttcc atacatgtct gtaccaataa tttcccgaat
     4621 cttcatgatt gatagttcct ttttgtaaga taaaagaact gttgaaacaa ctgccgcagc
     4681 acaatctgac tgatcgtgtt gtatcgtaca aggatatttt tttaacatag aaatactcaa
     4741 ttctttagaa ttaatctaac ttttctttga tgtcacccaa acctcaatca ctgcccagta
     4801 gttcttgaat tagtttattt tcaatcgtat cattctttgt tcttgatatc tttccaagca
     4861 ttgtagtaca gcagattttt gtccaaggac tgagcctcct ttaacaactc aagctccctt
     4921 acattatatc agccagcccc atggaaagat tatggccaga ctaatcaatg atataataca
     4981 tactagaata caaaatattt ttttattcat actttttctc ctatctttat tttctaccta
     5041 catcatacct cttattaagc tcattttaaa attacgttaa cttttttcac aaatttattt
     5101 cgactttctt ttttactttt tacattgcta tgatacaata tttttaacaa aattttattg
     5161 tttgttaact tttttcacga aaaggagaaa atcatgcgtt atgatttcgg aaaggtctat
     5221 aaagaaatac gtgagtcaaa ggggctgacc caagaagagg tctgtggggg tgttctctca
     5281 agaaccagct tatcaaaaat cgaaagtggc aagacaaccc caaaatatga aaatatggaa
     5341 tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg aatatatctg tcaactttat
     5401 caaccaagtc aacgcacaga gattatgcaa acctatctca atatgcgttc cattatcggg
     5461 accagcgatc ttgtcaattt atttcaaaaa tgccaagact acctcaagac ccaccacgat
     5521 ctccctatag aagaaatcag agatatgctt gaagttgtta tttatctccg tcaacacggg
     5581 gcaggggaac tatcaaaaca cgcagaacag gttgtcaaaa aactttggaa aaaaattgaa
     5641 aaacaagata catggtatga gagtgatcta aaaatcctca ataccattct tttcagtttt
     5701 cccattgaat acctccatct catcactgga aaaatattgc aacgcctaga agtctataaa
     5761 aactatcaac atttatatga cttgcgaatg acaattctat taaacctttc cacactctat
     5821 ctatacaatc aagataaaaa catgtgtaag caaatctgct acactttact ggaggacgcc
     5881 aagaataaga aaagctacga taggctggct atctgctatg tccgtattgg gatttgtagg
     5941 gatgatgcga aacttatcca aaaagggttc tcccttctgg agctaaccga ggaaacttct
     6001 atactgtctc atctcaaaaa agaagtagag atctattacc aagcgaagga aagataaaaa
     6061 agccgaggga tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc
     6121 attgttaagc cagaagtttt tagctctgcc aagccacata gtgtgtttac tactatgtgt
     6181 ccatgtcacc atgaatcgcc gaacgattta acgagaaagt atgactttta cgtttatccc
     6241 aactcaatta tgacattttt ttcaaaagtc aatatatctc actttttcaa cgacaagaaa
     6301 gaggctgcca atcgaccaac ctcttattct gaacccatca ctccatcact ttttagcttc
     6361 attcgctttc ttagcgactg caatctggta ttcgacttgg tcaaaccctg tgcctcccaa
     6421 ggaatttcgt ctttgaacag cagttttagg ttgcaaatag acatagatat cctcggtaat
     6481 gagagaatga taggtttgta attcctccaa agtccaatct tgaagatttt tagcagactt
     6541 gatagagtct agcactaatc ttcccacaac ctcatgagct tctctaaatg gcaagccttt
     6601 ccctgccaaa taatctgcca actcagttgc atttgaaaag tccttctctg ttgattcttg
     6661 cattttttcc ttgtttacct gcaaactaga taacatacct gccaacacat cgagggaatt
     6721 taaaatcgtt tcgaccgtat caaacattcc ttccttatct tcttgcaaat ccttattata
     6781 agctaaaggc aaggacttca tgactgtcaa cagtccaaac aaatgcccat aaactctgcc
     6841 agtctttcct cgaatcaact cagccatatc tggatttttc ttttggggca taatagagga
     6901 acctattgta aacgtatctg acaaggtaat gaattggtac tcaaaactac accaattaat
     6961 catttcttcg caaaaacgac tcatatgcat catgagtatg ctggcatttg atagaaattc
     7021 taagataaaa tcgcggtcac taacagcatc caaagaattt gtataaggtt gcttaaactc
     7081 cagcaaatcg ctagacaatt ggcgatcaat ggggaaagtc gttcctgcca aagctgccgc
     7141 acctaaaggg cataggtctg tatgcttctg gttaaattca aaacgttcac tgtctctttg
     7201 aaacatattg tagtaagcca tcaggtggta ggcaaaacta ataggctggg catgttgcag
     7261 atgggtatag cccggcatga tcgtcgccac atgattttcc gccaagtcta ataaaacacc
     7321 cttaagatga gccagtttat ctaggacata gcctagttgt tccttgagat acaagtgcat
     7381 atctgtcgca acttggtcat ttcgagaacg agccgtatgt aacttccctg caaggggacc
     7441 aattttttct gtcagcaaca cttccatatt catatgaata tcttcgtttg cgatatcaaa
     7501 gtcaagttga cctgcctcta gctcttctaa aagctctttc aagcctactt gaatcttttc
     7561 tgactcctcc aaactcaaaa tgcccgtctg acccaacatc tgaacgtggg ctaaagaacc
     7621 aatcacatca aatttagcta atttctggtc aaaggtatct gcactagtat acgtcgccaa
     7681 attttcatcg taaagagaat ttggtgattt ccgagccacg acctgagcgc tcccccttat
     7741 acagtttgac ttttgcagtc ccattgacaa ctttttgagt ctctttaata taagcaatca
     7801 aggcttgtgt agccgaacta aaccacaaag cattataaat gagatttgat aattcatttt
     7861 ctatgattgg tttaaaatga gccacttctc tcacaagcgt caagtcctca atttccttat
     7921 gagctgtcag cagggtcact gcacctggac actcatagat ttctcttgat ttaatcccaa
     7981 ccaagcgatt ttccacgtgg tcaatccgtc caaccccatg ttttccagct atttcattga
     8041 gtttttgaat caaatctgcc aattttaaca cttctccatt gagggaaact ggaacacctt
     8101 cactaaactc aatctcaatg tattctggca tatctggagc ttgctctgga gaagttgtga
     8161 tgccaaaggc ttcttctgga gcctgattcc atggattttc caaaatacca cactcattgg
     8221 cacgtcccca aagattttgg tcgacagagt agggattgtc aaggtcagca ggaacaggca
     8281 ccccattttt cttggcataa taaatttctt cctcccgaga ccatttccat tcacgaactg
     8341 gagcaatcac ttttaaattg ggatccaagg ctgcaatcga tacttcaaaa cgtacttggt
     8401 cattcccctt accggtacaa ccatgagcaa ttgtagtcgc tcctatctga tgcgctattt
     8461 caaccaattt tttagaaatc agagggcggc tcaaggcaga taccaaggga tacttttgtt
     8521 cataataggc atgtgactga tgagccacta gcacataatc tgtagcaaat tcgtccttaa
     8581 catcaatgac ataagattct actgccccaa ccttaagagc cttatcatgg atgaaatcta
     8641 agtctttccc ttcacccaca tccatacaaa ctgaaacaac atcatagtct ttctttaacc
     8701 aggtaatagc aactgatgtg tccaaaccgc cagaataggc caaaattact ttttctttac
     8761 tcatgttctt ttccttcttt ctatttttca atggatgctt taaaggcatc ctcaatgagt
     8821 ttgtctaatt ccccagactc cttcaacttt tgaatggttt tatcgactgc ctctttcaat
     8881 tccttgctat cttttttcat ggctaccgcg taggaatcat cttgctcttt ttcaaaattg
     8941 aggtctgcga ttgctaaatc aggattattt tccacaaatc ccttggaaac aggttcttca
     9001 aagataacgg catccacttg tcctgatttt aaatctgtga ttaaattccc atttttaggc
     9061 agagatacga gggaagaatt ttgtagcaaa tctttcgcca tcgtctcttg aatcgaacct
     9121 ttctgcgctc cgaccttttt ctgcgccaaa tcgttgacag actgataagt ggctaaatca
     9181 gattttttga caatgagttt attttttgca gtatagtagg gagttgaaaa atcaaacacc
     9241 ttgctccgtt catctgtctt agaaacacct gatatggcaa ggtcagcttt tcctgattga
     9301 acgctagcca gtacattgtc aaaactcata ggagatagtt ccaattcgac acctagttct
     9361 gttgcgatag ccttggctaa ttcgatatct gaacccacaa tctgattttt cccatcaacc
     9421 actttttgat attcaaatgg agcaaaatct ggattgaggg ctacaaccaa ttttcctttg
     9481 gacttgatgg cttcaattcc agccgattga ttgttactac aagcaaatag tagggggagc
     9541 atcactaaac caaacatcgt catcaacacc ttcttcatct tattcataac aaagcctcct
     9601 ttatgtttat tcttttttat tgtataaata atactcctat actcatcctt tgtcaagcct
     9661 aaattgaaaa aattttcaat ttgaaacaaa aaaaacctag aaatactaca taaaatgtca
     9721 ttcctaggcg tatttatgct atttttctct ggaaaacatg agtattcagc cagtcaaaag
     9781 gagctgaatg aactcatttt ccctcgccca attcaatgac acgatgacat tgttgggcta
     9841 cataagcatc gtgggtcaca ataatgactg ttttcccttc tcgattcatc tctaagagaa
     9901 acttcaagac caaatctcta ttttcaggat ccagcgaacc tgtaggttca tcggctaaaa
     9961 tcagctggct gggttttaag atggctctag caactgcaat tcgttgttgt tcgcccccag
    10021 acaactcgga gaccctttga tgcaaagtag ctgacaaacc tactctctct aaaatctctt
    10081 ccaccttttt gagcttgtct ttcttaggca atttcacata tttcagcgcc agcatgagat
    10141 tgtactcgac cgtttcatca tcaatcaggg caaaattttg aaacagataa gagatatgtt
    10201 cacggattat tgtttgcgac ttagcagaat taaccgctag atttgtctga ccaaaaatct
    10261 cataccgtcc gctataatca ccatctatca aacccaataa atttaacaag gtcgacttcc
    10321 cactaccact cttaccaaca atagctacca aatccccctg atcaatcctg agagataagt
    10381 tatccaaaat cacttttccc ccaatggttt tggtaatatt tttcaactca atcataagat
    10441 gccccctttc aataactcta ctagacttct tttctccatc ctagaagcta agcctagcac
    10501 aaatagtata tccagacatg taaaacctgc aaacagtaga agtggtaaga acgcatgggc
    10561 aaagaaaatc aagactagaa gagggaaact atagcccagc aagagcagaa cgaggagagg
    10621 acggtagcga tcgaccagtt tccaccccat aaacttcttg gtaatgatat ccctgcgctt
    10681 caataagaaa gttgttacta gtaagaagta ggaaatcatc atgctaagga gaccaaacaa
    10741 agcaaagagt aggttaaaat tccgaacagc atctcgataa gaatccactt tctcttgttg
    10801 aatggcttga atagatgaaa attttaaata atttccatct gacaatttct caactaactc
    10861 tgtaatctct ttttgatgtt gaaccgtatt ttcaatttta atcggattat ttaagccagt
    10921 tgttgacagg gaggctttct catcccacat catatcagaa tcattgacca agctaataat
    10981 tggattggag agattttcct ttcgcttatc actatatggg aaaaatgacc aatctccttc
    11041 ataataggca atctcgacat ccatctcctc tatcgttcgt ttttgctgct cttcatactt
    11101 catcgaatga aaggcaatta acttccccaa gagctgattt ttatcttctt cacctttcgt
    11161 acttgctggc atcaaaataa cttttttaat accggtatct ggtagcttga atcccttgct
    11221 ctttagaaaa ttgcgattgg catagtaaac atccaccgta tctgttaact gatattgctg
    11281 aatctgttct gattggacaa aattttttac aggaagactg ctactctgca catagcccgc
    11341 ctgcgttttt tctaccaaat cctgataaaa tcgatagaaa taatctgtag atttccctga
    11401 ccctgctagc tcttcttgcc acagattatc attgagtttg aaggtttcta aggtcaggta
    11461 attaccttga cttacccact gttgctgata agcaagttct ttgttttctt gttctaaact
    11521 tctgcccacc ccaatcagta aggccgtcag taaaatagtt gtccctattt tcatcacata
    11581 attgaagata agaccaaatt tgaaagatga aaaacctttc agcagagagc tgattgtcat
    11641 tttttggatt aagaggtaag tcaaccaact gataaagaga taaagctgca acagcaaaaa
    11701 atgagacaac cacagcatag gaaacaaatc ttttggctta taatcaagca agaaaaacac
    11761 gcctagattg atcacaagag ccccacctag gaggaggtaa aggttgcctt ttacaacatc
    11821 agctaaaaca gccctatctt gaaaaccaag taatttttgt accccaactc ttttcatctc
    11881 catcatcggt tgatacactg tcactaacac aagaagcaaa atagccaaga caaaaacaat
    11941 ggcagataaa agcaaatctc gatttatgac ttccactgca cttttgtagg tcggctctag
    12001 caaggtagcc tggtctatct tgaaaaaatc gctccatttc tgtacaatcc tatccttgtc
    12061 catctcttgt gtagaagtta tcgtatagcg accatttaaa ctacgagatg tatccttgat
    12121 ataggtttga aaagtcataa gctgaatagg tttggctttt agaaaggtcg gaatcgtacc
    12181 aagtttattg gaaatttctt tattactata gactccttca ccatctgtgg taaaatcaag
    12241 agaagaaatc ccaaactctt ggtaggggaa ggtatcttta tcaaaaacac cagacttgac
    12301 cacctcatca ccactgtctg ttttgatgat ggagacttta tactcctttg atacatcctc
    12361 aaaaaatcga agaacagacg ctgcaggttc gttaatatct ttcaaataca aatccaaaga
    12421 atctacagtc tttcccattt cttgaatccg aaccacttct cgtgtatagt ttacattaaa
    12481 aacaaaaaaa gtagtacaca gaagcaggag taacatacaa agatgactga tttttttcat
    12541 agagatacct cccgtgaaga aactccctct ccaaaagggg agggagaatt ttaattaaaa
    12601 tccatagaaa tattccattc ccgttggtgg gaatttgaag attgaagccc gagcccatgc
    12661 ctcagcttct gcacgatcct tagagaattt atttccatct ttaacagttg ctgtatgaga
    12721 gcgagtagaa tgtaaataat cagaataacc aaaatttcct gaccatccta ctccatagtt
    12781 ccattgacca ccatcaaccc atactgccaa aacacttgta gaagcaagta tactacatac
    12841 cattaaagat gcaacaccaa ttttctcatg atttatttct cctattatcg agagctattc
    12901 cgtagaaaca gctatagtaa tttgtcaaca agtatcgtag tccattggac tcaccttcct
    12961 tctatctcat ttcattttta aacaacgaac ttactcttaa ctagaagaat agttctcttc
    13021 tgtatcaggt ctttgaacaa ccagagtagt attaatcaca ctctaactat tactcaagaa
    13081 tcagcccaat aaacaatatg gatagcgctt tcaaattttt aaactcttat cccatccttt
    13141 tatctatata ataagtgaaa atataataac tgtcaagtaa tcgaagtgaa ttttataaaa
    13201 aaaattacaa gccaaatttg caaagtttac aataagccgc tagggcaatc gtctatcaga
    13261 atgtccgttt atttgtcaat aatccgagaa aatcttgcaa cgcttagaag tctataaaaa
    13321 ctatcaacat ttatatgact tgcgaatagc aatcctgctc aacctttcca cactctatct
    13381 atacaatcaa gataaaaaca tgtgtaagca aatctgctac actttactag aggacgccaa
    13441 gaataagaaa agctacgata ggctggttat ctgctatgtc cgtattggga tttgtacaga
    13501 cgattctaaa cttatccaaa aagggttctc ccttctggag ctaaccgagg aaacttctat
    13561 gctgtctcat ctcaaaaaag aagtagagat ctattaccaa gcgaaggaaa gataaaaaag
    13621 ccgagggatt tcctcgactc tttacatatt atttgttcgt tagttcttaa taccagccat
    13681 tgttaagcca gaagttttta gcagcagtcc atgaaccgta acgtcctgca acgtaggcat
    13741 ctgctacacg ttcttggttt tcagctgagt agtcaccgtt caggtatgaa tctgttaatt
    13801 ggtaacgtcc gatataacgt ccatttgtag ctgtatagct accacctgat tctttttgag
    13861 cgatccattc tttggcttct gcttcagatc cgcttacagt tgaaacaact gtttcactta
    13921 ctactggagc tgaaacagtt tcatcttgag cggctggtgc cgcataagta gctggcgctg
    13981 gtgttgcaac aggcgctaca gggccatcga taaccaactc ttgaccaaca taaatcaaat
    14041 gaatgttatc aatgtggttg ttttctgcca atttttcaac tgttgtgttg tgagtttcag
    14101 cgatttctga aagtgtatca ccttctttaa cagtgtaagt tgatgattct tgagcagata
    14161 caaatgatgg agcaaatact gcaaacaagg cagctactcc tgcaagagtt gctttaatct
    14221 ttttagttat tgatttcata ttcgaaaatt ctccttcttt ctatagttct atcataccct
    14281 ttaaatatta ccgtttcttg acacttttat gtagaaatat tacaaaatta ttttttattt
    14341 ccctaaataa ggtgtttttt gtcataaaag atactatttt tatgctattt gaggtattag
    14401 aaaggttttc atccacaatt aaagccaagc ccctcattct ttgatataat agagataaga
    14461 atttttataa ggggaaactg atgaaatcac ttcgttttca atctgtcttt gatatcatcg
    14521 gaccagttat gattggccca tctagtagcc ataccgctgg tgctgttcgt attgggaaga
    14581 ttgtctcttc catttttgat gatactccga cagaagttga attccaactt tttaactcat
    14641 ttgccaagac ctatcgtggt cacggaacag acctagccct tgttgcaggt attttaggca
    14701 tggatacaga tgatcctgaa attccaaaca gtctggagat tgcccacaag cgtggtatca
    14761 agattgtctg gaccattcag aaagacagca atgcccctca ccccaacacc actaaaatta
    14821 ccgtcaaaaa tgcccacaag accatcagcg tgactggtat ttctatcggt ggaggaaata
    14881 ttcaggtaac cgaactcaat ggttttgccg tctctctcaa tatgaataca ccgactatca
    14941 tcatcgttca tcaagatatt ccaggtatga ttgcccttgt tacagaggcg ctttcccgct
    15001 atggtatcaa tatcgcccag atgaatgtca ctcgtgaaaa agctggtgaa aaagccatta
    15061 tgattatcga agttgacagt cgcaactgtg atgaggctat cgaagaaatt cgaaaaatcc
    15121 ctcatctcca caatgtcaat ttctttaaat aggaggaagc atgttttatt ctatcaaaga
    15181 attggtcgag caagcagatc tggactttca aggaaatgtc gcagaactca tgattacaac
    15241 agagtttgaa ttgaccggtc gcgaacgtga agaagtcttc cttctcatgg aacgcaatct
    15301 ggaagttatg aaagcctctg tccaacttgg cctcaatgaa aataaatctc gtagtggcct
    15361 gacaggtgga gatgctgcca aattggatca ctacatcgaa aacggaaaaa ctctgtcaga
    15421 ttatacgatt ctctcggctg cccgaaatgc catcgcagtt aatgaacaca atgccaaaat
    15481 gggcttggtc tgtgccactc caaccgctgg aagtgctggc tgtctgccat ccgttctcac
    15541 tgctgctatt gaaaaattag acctcagcca cgagcaacaa ttggatttcc tctttgctgc
    15601 tggtgccttt ggactagtca tcgcaaacaa tgcctccatc tcaggtgctg agggtgggtg
    15661 tcaagctgaa gttggttcag cctctgctat gagtgctgcc gccttgactc tggctgcagg
    15721 tggaacacct tatcaggcca gtcaagctat tgcatttgtc attaaaaata tgctaggcct
    15781 catctgtgac cctgttgcag gtttggtcga agttccctgt gtcaaacgta atgccatggg
    15841 agctagcttt gctttcatcg cagcagacat ggccttggca ggtatcgaat ctaaaatccc
    15901 tgtggatgaa gtgatcgatg ccatgtacca agtaggagca agcatgccaa ctgcctttcg
    15961 tgaaacagct gaaggtggac tcgctaccac ccctactggt cgtcgcctcc aaaaagaaat
    16021 tttcggagaa taagcttatc aaataggaga aaccatgacc tctatcacag cgattttttt
    16081 cgatttggat ggaaccctcg ttgatagttc tatcgggatt cacaatgcct ttacctatac
    16141 ctttaaagag ctgggggtgc ctagccctga tgccaaaact attcgtggtt ttatgggacc
    16201 acctctcgaa agtagttttg cgacctgcct gtctaaagac caaatttctg aagccgtgca
    16261 gatatatcgt tcttactata aggcaaaagg catctatgaa gctcaactct ttcctcagat
    16321 tatagacttg cttgaggagt tatcgagcag ttatccactc tacatcacca cgacaaagaa
    16381 tacatctact gctcaagaca tggcaaaaaa cttggaaatc catcatttct ttgatggcat
    16441 ttatggttct agccctgaag caccccataa ggcagatgtc attcaccaag ccttgcagac
    16501 acatcaacta gcaccagaac aagccatcat catcggagat accaagtttg atatgctggg
    16561 agctcgagaa acaggtattc agaaattggc catcacttgg ggatttggag agcaagcaga
    16621 tctactaaac tatcaacctg attatatcgc tcacaaaccc ttagaagttt tggcgtattt
    16681 tcaatagcat agactgggta acaattccat ttcagaagaa aatgaagtaa tactcaatga
    16741 aaatcaaaga gcaaactagg aaactagctg cgggctgctc aaaacactgt tttgaggttg
    16801 cagatagaac tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg
    16861 aagtcagtaa ccatatacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag
    16921 tataagtctc tacataataa aacgcatagt accaaggtta acaaacttga tactatgcgt
    16981 ttttcttttt tattcaatgt gggttgtttg attagcaaag gcgataatag cttgcttcaa
    17041 ctcatctcca ctgagagtgc ggaactctcc aatcttttga ttgatggatt ctaaaggcat
    17101 gacattaacc ttaccaacga aaatcttatc cataacttga ttgtcaacga gttcggttga
    17161 taccaagagt tcttcgtaga gtttttcacc tgggcggatt ccaacttcaa cgattggaat
    17221 ttcactttca gtgtggccac ttagaagcac catcttcttg gccaagtcat aaatcttgac
    17281 tggtttgccc atatcaagga taaagacttc cccatctttg gcataagcac cagcatggat
    17341 aaccagacgg ctagcttctg gaatggtcat aaagtaacgg gtcatgcgga agtctgtcac
    17401 agttacaggc ccaccttcag caatctgacg ttcaaagact ggaatgacac taccacggct
    17461 accaagaaca ttcccaaaac gaactgcaca gtaggttgat tggctacgtt ggttaaaacc
    17521 agtgacaatc aactccgcca cgcgcttggt tgctcccata acatttggtg gattgactgc
    17581 cttatctgtc gaaatcataa ccatcttaga cactttagct tcatcaacag ccttagcaac
    17641 attgtaagtt ccacggatat tgtttttgaa ggcttctttt ggattgcgct ccatcatagg
    17701 aacatgcttg tgggctgccg catgataaac aatagcaggt ttgtactgct caaagacttg
    17761 caacaaacga tcatagtctt gaatgtccgc aatcacaggt acataatcaa tcccttggaa
    17821 cttacgaatc aattcatgat aaacaaggta gattgagttt tccccatgac cgagcaagac
    17881 aatgcgttca ggattgaagc gactaacttg acgacagatt tcagaaccga ttgaacctcc
    17941 agctcctgtg actaagatgg tcttacctgt cagttctgca cccagacgcg attcgtcaag
    18001 acggatttcc tgacgaccca aaaggtccgt aatatcaatt ttttggaagc cagtacctgc
    18061 ttggtgaagg ccctgaacaa cagtttcaac cttaggcatc ttgtaacatt tgacacccag
    18121 cttattacac atctgcaaga tacgctcata ttctgacgga tccagcgacg gaatcgcaac
    18181 gatgacacgc tcgatttgat ggcgtttggc taattcaggc agattgtcat aagagcccaa
    18241 aacaggaata ccaccaagtt tttgaccctt tttcttagaa tccttatcca aaataccgac
    18301 cagttctaat tcactggttg gatgttggta actatccata aagagagccc caccatcacc
    18361 ggcaccaatc aagaaggtcc gacggtgttc tccatcacca ctaccttttt tgcgtctgga
    18421 gtagattaac tgccaagtaa tccgtggcaa taaaatcaag aaggtactca acaagataaa
    18481 gagaatgata aaacggatgg agaagagtgg caagaaggca taacagatac tatatgacaa
    18541 gacactgcta gcagtcacac caaaaaagat tttcatgaaa tccgtaatct tgctgtaacg
    18601 actaatgctc gcgttcaacc cccaaaaacc aatcatcaat tgatagaaca ggaaggccaa
    18661 actcgtatag ataatgtagt caacaggtgc tggattaatc agcccataaa ataaaatata
    18721 agatacaatg atggaaacca ccatactgaa aatatcaaat attccccaga aaacctgctt
    18781 ttgttgttta tttaaaattt ccaccagatc aatcacataa tctgttagtt ttttattcat
//
LOCUS       DAVGJJ010000034        17898 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000034 DAVGJJ010000000
VERSION     DAVGJJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.034"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..5262
                     /locus_tag="VVE35_001093"
     CDS             <1..5262
                     /locus_tag="VVE35_001093"
                     /inference="COORDINATES: protein motif:HMM:NF016529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU3061297.1"
                     /translation="RKFSLGVASVMIGAAFFGTSPVLADSVQSGSTANLPADLATALA
                     TAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAEEKPKEDKPAAAKPE
                     TPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAGKPALFEKKGLTVDA
                     NGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWFWEYKSPTTSTWYRG
                     SRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVNDHLKNEKKILLKAG
                     TYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTIQSKVLKAVIDQAFP
                     RVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAEYLMKLRDDAHLINA
                     EMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISFLGNALVSVSSDQTG
                     AKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLAAGVWSNSQNSYGGG
                     SNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELPSAKVVITEDANA
                     DKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQNPFLMTLDGIKKINL
                     HTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVN
                     ASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKL
                     GDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFHHWAA
                     DLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLLGGYSMKDFEGWQGR
                     SDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYKWTPEMRVELVDTDN
                     NKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLSTDKEKMYYF
                     NTQTGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRS
                     KQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANDMLRIQGNKEKV
                     SLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYVKAYAH
                     NTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQATYFDEIRTFENNSSM
                     YGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKV
                     DDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGK
                     GEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGDTWVGIYSTGNASNT
                     RSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPTVAMTNYTKESMDAL
                     KEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAEDFASLTAPAQAQEGL
                     ANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRGSGSNGNLRDVKLVV
                     TDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYGDGGDKYQSAAELIF
                     TRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYATDNHLLTERMVEYF
                     ADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEIDRPETPEQILPATGE
                     SQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(5429..7588)
                     /locus_tag="VVE35_001094"
     CDS             complement(5429..7588)
                     /locus_tag="VVE35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU3061298.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7585..8181)
                     /gene="recU"
                     /locus_tag="VVE35_001095"
     CDS             complement(7585..8181)
                     /gene="recU"
                     /locus_tag="VVE35_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU3061299.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYHGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHKDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8247..8774
                     /locus_tag="VVE35_001096"
     CDS             8247..8774
                     /locus_tag="VVE35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU3061300.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8844..9173
                     /gene="gpsB"
                     /locus_tag="VVE35_001097"
     CDS             8844..9173
                     /gene="gpsB"
                     /locus_tag="VVE35_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU3061301.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9194..9574
                     /gene="rnpB"
                     /locus_tag="VVE35_001098"
     ncRNA           9194..9574
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVE35_001098"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9659..10816
                     /locus_tag="VVE35_001099"
     CDS             9659..10816
                     /locus_tag="VVE35_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU3061302.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10829..12223
                     /gene="mapZ"
                     /locus_tag="VVE35_001100"
     CDS             10829..12223
                     /gene="mapZ"
                     /locus_tag="VVE35_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU3061303.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12299..13723
                     /gene="gndA"
                     /locus_tag="VVE35_001101"
     CDS             12299..13723
                     /gene="gndA"
                     /locus_tag="VVE35_001101"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU3061304.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13735..14424
                     /locus_tag="VVE35_001102"
     CDS             13735..14424
                     /locus_tag="VVE35_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061305.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTIQE"
     gene            14522..15541
                     /gene="cbpC"
                     /locus_tag="VVE35_001103"
     CDS             14522..15541
                     /gene="cbpC"
                     /locus_tag="VVE35_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU3061306.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPTTGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMVTGWVKDDSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVQ"
     gene            complement(15659..16891)
                     /locus_tag="VVE35_001104"
     CDS             complement(15659..16891)
                     /locus_tag="VVE35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3061307.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALHFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(16934..17737)
                     /locus_tag="VVE35_001105"
                     /pseudo
     CDS             complement(16934..17737)
                     /locus_tag="VVE35_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632445.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
BASE COUNT         5488 a         3283 c         4077 g         5050 t
ORIGIN      
        1 cggaaatttt cattaggtgt tgcttctgtt atgattggag ctgcattctt tgggacaagt
       61 ccggttcttg cagatagcgt gcagtctggt tccacggcga acttaccagc tgatttagct
      121 actgctcttg caacagcaaa agagaatgat ggacgtgatt ttgaagcgcc taaggtggga
      181 gaagaccaag gttctccaga agttacagat ggacctaaga cagaagaaga actattagca
      241 cttgaaaaag aaaaaccggc tgaagaaaaa ccaaaagagg ataaacctgc agctgctaaa
      301 cctgaaacac ctaagacggt aacccctgaa tggcaaacgg tagagaaaaa agaacaaaag
      361 ggaacagtca ctatccgaga agaaaaaggt gtccgctaca accaactatc ctcaactgct
      421 caaaatgata acgcaggtaa accagccctg tttgaaaaga agggcttgac cgttgatgcc
      481 aatggaaatg caactgttga tttaaccttc aaagaagatt ctgaaaaggg caaatcacgc
      541 tttggtgtct tcctgaaatt taaagatacc aataataatg tttttgtcgg ttatgacaag
      601 gatggctggt tctgggagta taaatctcca acaactagca cttggtatag aggtagtcgt
      661 gttgctgctc ctgaaacagg atcaataaac cgtctttcta tcactctcaa gtcagacggt
      721 cagctaaatg ccagcaataa cgatgtcaat ctctttgaca cagtgactct accagctgcg
      781 gtcaatgacc atcttaaaaa tgagaagaag atccttctca aggcaggaac ttatggcaat
      841 gaccgtacgg ttgtcagcgt taaaacagac aatcaagaag gcgtaaaagc ggatgatact
      901 cctgcccaga aagaaacagg tccggttgtt gacgatagca aggtgactta tgatacgatc
      961 cagtctaagg tcctcaaagc agtgattgac caagccttcc ctcgtgtcaa ggaatacagc
     1021 ttgaacgggc atactttgcc aggacaagtg caacagttca accaagtctt tatcaataac
     1081 caccgaatca cccctgaagt cacttataag aaaatcaatg agacaacagc agagtacttg
     1141 atgaagcttc gcgatgatgc tcacttaatc aatgcggaaa tgacagtacg cctgcaagtt
     1201 gtggacaatc aattgcactt tgatgtgacc aagattgtca accacaatca agtcactcca
     1261 ggtcaaaaga ttgatgacga aagaaaacta ctttcttcta ttagtttcct cggcaatgct
     1321 ttagtctctg tttctagtga tcaaactggt gctaagtttg atggggcaac catgtcaaac
     1381 aatacgcatg tcagcggaga tgatcatatc gatgtaacca atccaatgaa agacctagcc
     1441 aagggttaca tgtatggatt tgtttctaca gataagcttg ctgctggtgt ttggagtaac
     1501 tctcaaaaca gctatggtgg tggttcgaat gactggactc gtttgacagc ttataaagaa
     1561 acagtcggaa atgccaacta tgtaggaatc cacagctctg aatggcaatg ggaaaaagct
     1621 tataagggca ttgttttccc agaatacacg aaggaacttc cgagtgctaa ggttgttatc
     1681 actgaagatg ccaatgcaga caagaaagtt gattggcaag atggtgccat tgcttatcgt
     1741 agcattatga acaaccctca aggttgggaa aaagttaagg atatcacagc ttaccgtatc
     1801 gcgatgaact ttggttctca agcacaaaac ccattcctta tgaccttgga tggtatcaag
     1861 aaaatcaatc tccacacaga tggtcttggg caaggtgttc tccttaaagg atatggtagt
     1921 gaaggtcacg actctggtca cttgaactat gctgatattg gtaagcgtat cggtggtgtc
     1981 gaagacttca agaccctaat tgagaaggct aagaaatatg gagctcatct aggtatccac
     2041 gttaacgctt cagaaactta tcctgagtct aaatacttca atgaaaaaat tctccgtaag
     2101 aatccagatg gaagctatag ctatggttgg aactggctag atcaaggtat caacattgat
     2161 gctgcctatg acctagctca tggtcgtttg gcacgttggg aagatttgaa gaaaaaactt
     2221 ggtgacggtc tcgactttat ctatgtggac gtttggggta atggtcaatc aggtgataac
     2281 ggtgcctggg ctacccacgt tcttgctaaa gaaattaaca aacaaggctg gcgctttgcg
     2341 atcgagtggg gccatggtgg tgagtacgac tctaccttcc atcactgggc agctgacttg
     2401 acctacggtg gctacaccaa taaaggtatc aacagtgcca tcacccgctt tatccgtaac
     2461 caccaaaaag atgcttgggt aggggactac agaagttatg gtggtgcagc caactatcca
     2521 ctgctaggtg gctacagcat gaaagacttt gaaggctggc aaggaagaag tgactacaat
     2581 ggctatgtaa ccaacttatt tgctcatgac gtcatgacca agtacttcca acacttcact
     2641 gtaagtaaat gggaaaatgg tacaccggtg actatgaccg ataacggtag cacctataaa
     2701 tggactccag aaatgcgagt ggaattggta gatactgaca ataataaagt agttgtaact
     2761 cgtaagtcaa atgatgtcaa tagcccacaa tatcgcgaac gtacagtaac tctcaacgga
     2821 cgtgtcatcc aagatggttc agcttacttg actccttgga actgggatgc aaatggtaag
     2881 aaactttcta ctgataagga aaagatgtac tacttcaata cgcagactgg tgcaacaact
     2941 tggacccttc caagcgattg ggcaaagagc aaggtttacc tttacaagct aactgaccaa
     3001 ggtaagacag aagagcaaga actaactgta aaagatggta aaattaccct agatcttcta
     3061 gcaaatcaac catacgttct ctatcgttcg aaacaaacca atcctgaaat gtcatggagt
     3121 gaaggcatgc acatctatga tcaaggattt aatagcggta ctttgaaaca ttggaccatt
     3181 tcaggcgatg cttctaaggc agaaattgtc aagtctcaag gggcaaacga tatgcttcgt
     3241 attcaaggaa acaaagaaaa agttagtctc actcagaaat taactggctt gaaaccaaat
     3301 accaagtatg ccgtttatgt cggtgtcgat aaccgtagta atgccaaggc aagtatcact
     3361 gtgaatactg gtgaaaaaga agtgactact tataccaata agtctctcgc cctcaactat
     3421 gtaaaagcct acgcccacaa tacacgtcgt aacaatgcta cagttgacga tacaagttac
     3481 ttccaaaaca tgtacgcctt ctttacaact ggatcggacg tctcaaatgt tactctgaca
     3541 ttgagtcgtg aagctggtga tcaagcaact tactttgatg aaattcgtac ctttgaaaac
     3601 aattcaagca tgtacggaga caagcatgat acaggtaaag gcaccttcaa gcaagacttt
     3661 gaaaatgttg ctcagggtat cttcccattt gtagtgggtg gtgtcgaagg tgttgaagat
     3721 aaccgcactc acttgtctga aaaacacgat ccatatacac aacgtggttg gaatggtaag
     3781 aaagtcgatg atgttatcga aggaaattgg tcactcaaga caaatggact agtgagccgt
     3841 cgtaacttgg tttaccaaac catcccacaa aacttccgtt ttgaagcagg taagacctac
     3901 cgtgtaacct ttgaatacga agcaggttca gacaatacct atgcttttgt agtcggtaag
     3961 ggagaattcc agtcaggtcg tcgtggtact caagcaagca acttggaaat gcatgaattg
     4021 ccaaatactt ggacagattc taagaaagcc aagaaggcaa ccttccttgt gacaggtgca
     4081 gaaacagggg atacttgggt aggtatctac tcaactggca atgcaagtaa tactcgtagt
     4141 gattctggtg gaaatgccaa cttccgtggt tataacgact tcatgatgga taatcttcaa
     4201 atcgaagaaa ttaccctaac aggtaagatg ttgacagaaa atgctctgaa gaactacttg
     4261 ccaacggttg ccatgactaa ctacaccaaa gagtctatgg atgctttgaa agaggcggtc
     4321 tttaacctca gtcaggccga tgatgatatc agtgtggaag aagcgcgtgc agagattgcc
     4381 aagattgaag ccttgaagaa tgctttggtt cagaagaaaa cggctttggt agcagaagac
     4441 tttgcaagtc ttacagctcc tgctcaggct caagaaggtc ttgcaaatgc ctttgatgga
     4501 aacttatcta gtttatggca tacatcatgg ggcggaggag atgtaggcaa gcctgcaacc
     4561 atggtcttga aagaagcaac tgaaatcact ggacttcgtt atgttccacg tggatcaggt
     4621 tcaaatggta acttgcgtga tgtgaaactt gttgtgacag atgagtctgg caaggagcat
     4681 acctttactg caactgattg gccagataac aataagccaa aagacattga ttttggtaag
     4741 acaattaagg ctaagaaaat tgtccttaca ggtactaaga cttacggaga tggtggcgat
     4801 aaataccaat ctgcagcgga acttatcttt actcgtccac aggtagcaga aacacctctt
     4861 gacttgtcag gctatgaagc agctttggct aaggctcaga aattaacaga caaagacaat
     4921 caagaggaag tagctagcgt tcaggcaagc atgaaatatg cgacggataa ccatctcttg
     4981 acggaaagaa tggtggaata ctttgcagat tatctcaacc aattaaaaga ttctgctacg
     5041 aaaccagatg ctccaactgt agagaaacct gagtttaaac ttagctcttt agtttccaat
     5101 caaggtaaga cgccagatta taagcaagaa atagatagac cagaaacacc tgaacaaatc
     5161 ttgccagcaa caggtgagag tcaatctgac acagccctct tcctagcaag tgttagtcta
     5221 gccctatctg ctctctttgt agtaaaaacg aagaaagact agtatttagt aaaacctctt
     5281 aacaagatta cggaagcagt ctctatcttt tccaatgagg tttatagtac agaaaaagcc
     5341 tgagaagatg tcttctcagg cttttgttaa gcacataaat acaatagtgc tatgacaaaa
     5401 tcacccagaa aaatctgggt gataaatgtt atggttgtgc tggttgagga ttctgatttt
     5461 gttgatcagg ggttgtattt gattgttgcg tattattgtt aggattggta gtcgtactat
     5521 tatttgtgct tggagtggtt gagctagact gtgaagttga actatctgat gatgagcttg
     5581 aactttcagt tgatgggggt tgttgtggag caggtgagtt ccacgtagaa cgagcaccat
     5641 ttttaaatac gaattctcca tttctgtaga gcccctctgg tatattccaa tcctctggat
     5701 tgcttccttc agacagatag gtcatcatag agcggtaaac tttggcagcg accgtaaggc
     5761 cattgcctac aagtggtgtc agacggttag aatagcctgt ccatacagcc attgaatatt
     5821 tacgcgtata gccagcaaat agttcatcag gtgctacaaa ttgagaggtc ttgatgtggt
     5881 tttcaatttc ctcgtctgta tagttagagg ttcctgtttt accagcctga gggagccaag
     5941 caagataggc atttcgtcca gttccataag tcaagactgt tttcatcatg tcggtcatca
     6001 tataggctgt cgtttccttc atggcacgag ttccgacatt agagaactct ttttcactcc
     6061 catcactaaa gacgacttta tggatataca ttggtttata gtaagttcca ccatttgcaa
     6121 aggcagcgta agcagcagcc atcttttcac tacttgctcc atattttttg tctgattcgg
     6181 ttgtgttact tgaaatggca tttgagtagt gaatacttgg gtagtcgatt ccgagaccat
     6241 ttaggaaagt cttggcgcgg ttgagtccga ccttgtttag agtttccacg gctgggacgt
     6301 ttcgcgattg ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc ctatcccagt
     6361 tataaacagg ggtatttgtc ccagggtagt tatagggttc atcgtgaacg atagtagcag
     6421 ttgaatcgta gacaccgtac tccaaggcag gagcatagtc tgtgatcggt ttcatagttg
     6481 atccccagtc gcggtttgtt tctactgctt ggttaattcc gaaggaaaca ttacttgact
     6541 gatggcgtgc tcctagctgg gcaatgactt taccgttaga aacatcaaca atggtagaag
     6601 cgacttgcaa ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa atatcccaca
     6661 gatgtttttg agcttcttgg tctacatttg tgtagacatc cattccagtt gtgagcaggt
     6721 tatagcctgt ttcttcttca acttgattga tgacttcctt gaggtaatta tccatgtaag
     6781 cagggtaatt acttgctgat ttgagacttt gtagtccatc agtaattggt gtattgactg
     6841 ctttctcata ctgttcagca gagatgtagc cttgattttt catttcagat aagaccaagt
     6901 ttcggcggtc ttgggctgct tctggatgtg aataggggtc atattggttt ggtgcctgag
     6961 gcattccagc cagcaaggct aactgaggta aacttaaatt attgaggtct ttaccatagt
     7021 agttttgagc tgctgtctgc attccatagt tcccattaga catgtagacc ttatttatat
     7081 agtaggtcaa gatttcttgc ttggttgctt tttgttctaa ctgaatcgct aaccaagctt
     7141 cctgagcctt acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa gtcaacttaa
     7201 tcaactgttg ggtgagagtt gatccacctt ggagggaatt gctttgcaga ttgcgcaaga
     7261 aagctcccag gatacggatg gtatcaatcc ccctgtggtc gaagaagcga tggtcttcga
     7321 tagaaacgat tgccttaacc aaatctgtgg gaatatcatt agcttgggca ttgacgcggc
     7381 gttcagaacc caagtcagca atgagttgat ttttattgtc gtagatttta ctagaagttg
     7441 ttgcaactag tttactctcg gataggctag gagccttgct aacgtagtag aaaaaaactc
     7501 ctccgcctaa gacaatggct gcgataacca agcttaagaa gctaatgctc agatacttga
     7561 ttaggcgcag aatcgttggt ttgttcatct tgttttacca cctaataaat gttctttgat
     7621 aacattgaga taaggaattt gagggaaggc accagccttg atttcatatc catattctcg
     7681 aatatattca agtggcattg atttttgtcc cttatcttta tgatagaagc gaatcaaatc
     7741 gaatgccggc aataagtagg tttcttgctg agaagaaaag tgaagaagga caaagcagat
     7801 tccttgttgg gcaaggactt gttccatatg ctgaatctga tgtggatgaa aatttttcat
     7861 cggaatcgca cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat atccatgata
     7921 aacgccagaa tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa tcttggcacg
     7981 acttcgttgt ggatagtcca cttgtacgat ttgaatagga gttggtttct tatgtataac
     8041 agccaagccc tgagacaaat agtagtcgtt ggtagcattg atcatctttt caaaagacat
     8101 tcctcgattt gcgaaatttt tgggttgaga aagagatgtt tgtctttttt gtgatgaaac
     8161 tttatgtgga tagttgacca taattctcct tattggtaca ataacatcac tttattatac
     8221 cataatttgc acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc tattctgctt
     8281 ttgatttggg tttattttct gataaggatc ctcgtcttaa attgattaaa aaagcgatac
     8341 gtaaagattt agaggctatg gcagcagatg gggtgtcttg gcttgtgttt acagggagtt
     8401 tgggttttga atattgggtt ttagaggttg ctcaggagat gaaaactgaa tacggtttcc
     8461 agttggccac catttttgct tttgaaaccc atggggaaaa ttggaatgaa ggcaatcaga
     8521 tgaaactgag tcgctttaag caggtagact ttgtcaaata tgcctatcct cgctatgaac
     8581 acaaggggca gttaagagat taccagcagt ttttgctgga aaacacgact agttcctatc
     8641 ttttttatga tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag atgaaaaatc
     8701 aagaggacta ttttataaag agattaacat ttgatcaatt aaatgaactt gctgaaaatt
     8761 tttccgaaaa ttgaagcttt gaccttgatt tttgtttgcc tttttttata taataatact
     8821 agcaagcgag aatggagaga gacatggcaa gtattatttt ttcagcgaaa gatatttttg
     8881 aacaagagtt tggacgtgaa gtccgtggct ataataaagt agaagttgac gagtttttag
     8941 acgatgtcat caaggactat gaaacctatg ctgccttggt caagtcactt cgtcaggaaa
     9001 ttgcggattt gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt caagcagaac
     9061 cccttgaagc ggcaattaca agttctatga cgaattttga tattttgaaa cgcctgaata
     9121 gattggaaaa agaagttttt ggtaaacaaa ttttagataa ctcagatttt taagtagtta
     9181 tttgagatgt gcaatttttg gataatcgcg tgaggagaat tgcttctcat gaggaaagtc
     9241 catgctagca caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca taagcctagg
     9301 gacgagaaat cgttacggca gttgaaatgg ctaagtcctt ggataggcca gagtaggctt
     9361 gaaagtgcca cagtgacgaa gtctttctgg aaacagagag agtggaacgc ggtaaacccc
     9421 tcaagctagc aacccaaatt ttggtcgggg catggagtac gcggaaacga acgtagtatt
     9481 ctgactgcta tcagctagag ctgttagtgg tagacagatg attatcgaag gaagtggtcc
     9541 tagtcacttc tggaacaaaa catggcttat agaaaattgc atataggttg gggctgagaa
     9601 attttctcaa cctcattttt taaagtggac atatagaaag gtcttgcaag actgtaacat
     9661 gaaaaaagaa tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg tcgttggtcg
     9721 tgaagtgcga gagttgggct acgattgtca gattgaaaat ggacgtgttc gttttcaagg
     9781 agacgtgaga gctattatcg aaaccaacct ttggcttcgg gcagcagatc gtatcaaaat
     9841 tatcgtagga acgttccctg ctaagacttt tgaagagcta tttcagggag ttttcgcttt
     9901 agattgggaa aattatttac cacttggagc tcggttcccg atttcaaaag ctaaatgtgt
     9961 taagtccaaa cttcacaatg agcccagtgt tcaggctatt tctaagaaag ctgttgtcaa
    10021 gaaattgcag aaacactatg ctcgcccaga aggggttcct ctgatggaga atggcccaga
    10081 gtttaagatt gaggtctcta ttctcaaaga tgtggcaact gtcatgattg atacgaccgg
    10141 gtctagcctc tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta tcaaggaaaa
    10201 tatggcagca gccattttac aactttctaa ctggtatcca gacaagcctt tgattgatcc
    10261 gacctgtggt tcggggactt tctgtattga ggcagttatg attgctagaa agatggcgcc
    10321 aggtcttcgt cgctcttttg catttgagga atggaactgg atcagcgatc gcttgattca
    10381 agaagtgcgc acagaagcgg ctaaaaaagt agaccgtgag cttgagctgg atatcatggg
    10441 ctgtgatatt gatgctcgca tggtggaaat tgctaaggcc aatgctcagg cagctggtgt
    10501 tgcaggagac attactttta agcagatgcg cgtgcaggat ttacgttccg ataaaatcaa
    10561 tggagtaatc atttccaatc cgccttatgg tgaacgtttg tcagatgatg caggggtgac
    10621 caagctctat gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga gcaaatttat
    10681 cctgactagt gatgaagctt ttgaaagcaa gtatggtagc caagcagata agaagcgtaa
    10741 gttatacaac ggaaccttga aagtggatct atatcaatat tttggtcagc gtgtcaaacg
    10801 gcaagaggta aaatagaaag ggatactcat gagtaaaaaa agacgaaatc gtcataaaaa
    10861 agaaggtcaa gaaccgcaat ttgattttga tgaagcaaaa gagctaacag ttggtcaagc
    10921 tattcgtaaa aatgaagaag tggaagcagg agtcttgcct gaggattcca ttttggacaa
    10981 gtatgttaag caacacagag atgaaattga ggcggataag tttgcgactc gtcaatacaa
    11041 aaaagaggag ttcgttgaaa ctcagagtct ggatgattta attcaagaga tgcgtgaggc
    11101 tgtagagaag tcagaagctt cttcggagga agttccatct tctgaagaca tcttactacc
    11161 cttgcctctg gacgatgagg agcaaggctt ggatcctcta ttgctagatg atgaaaatcc
    11221 aacagaaatg actgaagaag tggaagagga gcaaaacctt tctcgtctgg atcaagagga
    11281 ctcagaaaag aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg tatcagtaat
    11341 tatttgtgtc agtgcttatt atgtctaccg tcaagtggct cgttcgacca aggaaattga
    11401 aacttctcaa tcaactacag ccaatcaatc ggatgtggat gattttaata cactttatga
    11461 cgccttttac acagatagca ataaaacggc tttgaaaaat agccagtttg ataaactgaa
    11521 tcaactcaag actttacttg ataagctgga aggtagtcgt gaacatacgc ttgccaaatc
    11581 taaatatgat agtctagcaa cgcaaatcaa ggctattcaa gatgtcaatg ctcaatttga
    11641 gaaaccagct attgtggatg gtgtgttgga tgccaatgcc aaagccaaat cggatgctaa
    11701 atttacggat attaaaactg gaaatacgga gcttgataaa gtgctagata aggctatcag
    11761 tcttggtaag agccagcaaa caagtacttc tagctcaagt tcaagtcaaa ctagcagctc
    11821 aagttcaagt caagcaagct caaatacgac aagtgagcca aaaccaagta gttcaaatga
    11881 gactagaagt agtcgcagtg aagtcaatat gggtctctcg agtgcagggg ttgctgttca
    11941 aagaagtgcc agtcgtgttg cctataatca gtctgctatt gatgatagta ataactctgc
    12001 ctgggatttt gcggatggtg tcttagaaca aattctagcg acttcacgtt cacgtggcta
    12061 tatcactgga gaccaatata tccttgaacg tgtcaatatc gttaacggca atggttatta
    12121 caacctctat aagccagatg gaacctatct ctttaccctt aactgtaaga caggatattt
    12181 tgtcggaaat ggcgctggtc atgcggatga cttggattac taagcagtcg ttacaaaatt
    12241 ctttcttttc aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg agtgtcaaat
    12301 gacaaaagct aactttggtg ttgtaggtat ggccgtaatg ggtcgtaacc ttgcccttaa
    12361 tattgaatct cgtggttaca cagttgctat ctacaaccgt agtaaagaaa aaacggaaga
    12421 tgtgattgct tgccatcctg aaaagaactt tgtaccaagc tatgacgttg aaagttttgt
    12481 aaactcaatc gaaaaacctc gtcgtatcat gctgatggtt caagctggac ctggtacaga
    12541 tgctactatc caagcccttc ttccgcacct tgacaagggt gatatcttga ttgacggtgg
    12601 aaatactttc tacaaagata ccatccgtcg taatgaagaa ttggcaaact caggtatcaa
    12661 ctttatcggt actggagttt ctggtggtga aaaaggtgcc cttgaaggtc cttctatcat
    12721 gcctggtgga caaaaagagg cctacgaatt ggttgcggat gttcttgaag aaatctcagc
    12781 taaagcacca gaagatggca aaccatgtgt gacttacatc ggtcctgatg gagctggtca
    12841 ctatgtgaaa atggttcaca atggtattga gtacggtgat atgcaattga tcgcagaaag
    12901 ctatgacttg atgcaacact tgctaggtct ttctgcagag gatatggctg aaatctttac
    12961 tgagtggaac aagggtgaat tggatagcta cttgatcgaa atcacagctg atatcttgag
    13021 ccgtaaagac gatgaaggcc aagatggacc aatcgtagac tacatccttg atgctgcagg
    13081 taacaaggga actggtaaat ggactagcca atcatctctt gaccttggtg taccattgtc
    13141 actgattact gagtcagtgt ttgcacgcta catttcaact tacaaagaag aacgtgtaca
    13201 tgctagcaag gtgcttccaa aaccagctgc cttcaacttt gaaggagaca aggctgaatt
    13261 gattgaaaag atccgtcaag ccctttactt ctcaaaaatc atttcatacg cacaaggatt
    13321 tgctcaattg cgtgtagcct ctaaagaaaa caactggaac ttgccatttg cagatatcgc
    13381 atctatctgg cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga ttacagatgc
    13441 ttacaaccgc gatgcagatc ttgccaacct tcttttggac gagtacttct tggatgttac
    13501 tgctaagtac caacaagcag tacgtgatat cgtagctctt gcggttcaag caggtgtgcc
    13561 agtgccaact ttctcagcag ctattactta ctttgatagc taccgttcag ctgaccttcc
    13621 agctaacttg atccaagcac aacgtgacta ctttggtgct cacacttacc aacgtaaaga
    13681 caaagaagga accttccact actcttggta tgacgaaaaa taagtaggtc agccatgggg
    13741 aaacggattt tattacttga gaaagaacga aatctagctc attttttaag tttggaactc
    13801 cagaaagagc agtatcgggt tgatctggta gaggaggggc aaaaagccct ctccatggct
    13861 cttcagacag actatgattt gattttattg aacgttaatc tgggagatat gatggctcag
    13921 gattttgcag aaaaattgag ccgaactaaa cctgcctcag tcatcatgat tttagatcat
    13981 tgggaagact tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc atacatctat
    14041 aagccagtcc ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg aggtcgggac
    14101 ttcattgatc aacactgcag tctgatgaaa gttccaagga cctaccgcaa tcttaggata
    14161 gatgttgaac atcacacggt ttatcgtggt gaagagatga ttgctctgac ccgccgtgag
    14221 tatgaccttt tggcgacact tatgggaagc aagaaagtat tgactcgtga gcaattgttg
    14281 gaaagtgttt ggaagtatga aagtgcgacc gagacaaata tcgtagatgt ctatatccgc
    14341 tatctacgga gcaagcttga tgttaaaggc caaaaaagct acattaaaac cgtgcgtggt
    14401 gttggatata ccattcaaga atagataagc agttgcagtt gtgtaactgc ttttttgagg
    14461 agtttttata tattgacatg cagtcgggtc tttgctacaa tcgttttagg aggatatata
    14521 gatgaaactt ttgaaaaaaa tgatgcaagt tctactagca gtctttttct ttggtttgct
    14581 agctacaaat acggtatttg cgaataccac aggtggccga tttgttgata aggataatag
    14641 aaaatattat gtaaaagatg atcataaagc aatctattgg cataaaatag acggtaaaac
    14701 ttactatttt ggtgatattg gagagatggt tgtcggttgg caatacttag aaattcctgg
    14761 aacaggttat cgtgatgatt tatttgataa tcgtccagtc ttcgaaattg gccttcagga
    14821 gaagtggtac tattttggac aagatggtgc tttgctagaa caaacagata aacaagtact
    14881 agaggcaaaa acgtctgaaa atacaggaaa agtatacggt gaacaatatc ctctatctgc
    14941 tgaaaagaga acttattatt ttgataataa ttatgctgta aagacaggct ggatttatga
    15001 agagggtcat tggtattatt taaataagct aggaaatttt ggcgatgatt cttacaatcc
    15061 actaccaatt ggtgaagttg ctaagggttg gactcaagat tttcatgtta ctattgacat
    15121 tgatagaagc aaacctgctc catggtacta cctagatcca acaactggta ttatgcaaac
    15181 aggttggcaa tatctaggta ataagtggta ctacctccgt tcctcaggag caatggccac
    15241 tggctggtat caggaaggta ccacttggta ttatttagac cagccaaatg gcgatatgaa
    15301 aacaggatgg caaaaccttg ggaacaaatg gtactatctc cgttcgtcag gcgccatggt
    15361 gactggctgg gtgaaagatg actcaacttg gtattaccta aatgcaggta atggagacat
    15421 gaagacaggt tggttccagg tcaatggcag atggtactac gcttatagct caggtgcctt
    15481 ggcagtgaat acgaccgtag atggctattc tgtcaactat aatggcgaat gggttcagta
    15541 atgacagggc gcttataatt atattagaac actcgcttaa tcaaagaagt gactttgttg
    15601 tacttgacct gacgattggg gagattaaaa gcatgtgaaa tctacacatg ctttttcgtt
    15661 agttatgagt gttgaacttg actaaccatt gacaaaagat aagagcaatt aaaataagat
    15721 acaaaaatat caatgctaac tgcaccgaaa cgcccgatgc tatggatagg gctaataggg
    15781 agagcaggga cggggcgaga atgtccactg tgctcagagc agagcccacc gtccccatct
    15841 tgtcactggg gatatgggca aagacagctg cttgtaggcg agggctgaga atgccgataa
    15901 tcgtagaact gatgaaactg gtcatgaaaa ttacgatata ggcttgacaa aggatactag
    15961 ttattagagt caatagggaa atctcacaga aaaccaagag gtgcgtgaaa cggatatttt
    16021 taaatagatt actgctggtc atattgccaa ggatagctcc acaagacatg atgatggaga
    16081 gcagggcgag gagttgccca atgctcagtt gtgctagtgt ctgttggatg aagtggaggg
    16141 caatcagccg aggggacaca tccaagatgg caatcatact ggtcgtcaga aacagtagta
    16201 tgacggtatc ttttaacctc aggagcacag ccagcgactg tttaagatgt tgaaaatatt
    16261 tcttaaaact cagtgctgta tgtgacattt caattctttt ttcaacctca tacaaggtat
    16321 gtcgaataac atacaggccc aaaaaggcaa tgacaaaagt cagagtgttg ataagggaaa
    16381 tagtttgaat acttataaca ttgataagga atgcgccagc cagattggcg acaatacgga
    16441 caaccctcat cagagactgc ctgaacccca tggcatcatg caggtcgtcc ttaattacac
    16501 tgatgtagag ggcgttcatc atgtaggcag acaggtagct caaggtgtca gagatgaggt
    16561 tgatgatgac aatcataatg actgaaacta atgaaaaggg cgacaagacg agaaatactg
    16621 tcaaaatagc gtagagcagg attttgattt tggcaatcca gagtgcgtgt ttgactctat
    16681 tttcttgaaa atctcctaga atcccgagaa agagttgcga tagtagggga accatctctg
    16741 aaatggaaat cagtaaaatc gctagcggag cgaaattgta ctgagccaca taattgataa
    16801 tggcgaggta gaaaatactg tcgccgaatg atgaaaaaca gctgtttagc agcaactgtc
    16861 tatatatgtt gtttgtccaa aataatttca tacatttctc cttatgtctg cacatcgaat
    16921 gggaatttat tatttaatag acaccaatat accccatgct tcctattccg aagtcaaaca
    16981 aatctgcttt accactatct ccttgaaaca tcagtaaacc gtgatcatct cgttcagaac
    17041 atgctaaaat tacctgttgg atttttttca tcagtaattt gttttgattg tagacagtgg
    17101 tttgtagtaa gctagttatt ccgtggcaat atcctgtcat catttttaga tgatggttaa
    17161 aaacaaattc ttgatattta ctaataatat ctttgtttcc gtcacaatcg tacatacaaa
    17221 gatataagat tattccggaa attccttcgc accaagaaag ttgtaaattt cctaaatttt
    17281 cagtattaga agtaactttt tctaataatt tttttaattc agtatggaat gtatggagat
    17341 cattatagaa catagaaggt tctaagactt tagaataggc aaataaagca tatgcaattc
    17401 cacaatatcc atgagcaaag ctatagtcta aaatgctttc ttcggctgta tttctttgta
    17461 ggaaataatg ctaaatttcc cctatactat tgtggatgaa aattttaaga gtattgtcat
    17521 tggtaaattg atagtatttt attaatgata ataggacacc agattttctc aatgcaaagt
    17581 ctatttcctc tagagtatca taattctcta ttaatttctt agaggtgacg tttgctaatt
    17641 gtagatagta ttgatattca tatgtgtgtg tcgttctaat tgtcactgcc ttcttgatga
    17701 tagagagtat tttagaatcc agttctgggt ggtacaattt atttagagga aacagtagtc
    17761 cagctattcc gtgttggaat gataaattac ttacgaaatt tccaaattct gttgaagaag
    17821 tgatccttcc ttcagaattt acagttttat ctagtaaaaa tcgtagaaca ctattacatt
    17881 ctgttttgta atcatatt
//
LOCUS       DAVGJJ010000035        17642 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000035 DAVGJJ010000000
VERSION     DAVGJJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.035"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1455
                     /locus_tag="VVE35_001106"
     CDS             <1..1455
                     /locus_tag="VVE35_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3061308.1"
                     /translation="HRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAF
                     PDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFY
                     ISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPF
                     ENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQ
                     SLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDS
                     YVEKSYHLLSDFIDQISVKYQIEIENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNT
                     IRNFQNIFPKFVSDVKKELSHYLETLEVCSSSMMVNHLSYTFVTHTKHLVINLLQNQP
                     KLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSPYDIIISNFI
                     IPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1817..2812
                     /locus_tag="VVE35_001107"
     CDS             1817..2812
                     /locus_tag="VVE35_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3061309.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            2939..4249
                     /locus_tag="VVE35_001108"
     CDS             2939..4249
                     /locus_tag="VVE35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU3061310.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4345..5247
                     /locus_tag="VVE35_001109"
     CDS             4345..5247
                     /locus_tag="VVE35_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3061311.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGFENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5249..6094
                     /locus_tag="VVE35_001110"
     CDS             5249..6094
                     /locus_tag="VVE35_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3061312.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRFAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6104..7423
                     /locus_tag="VVE35_001111"
     CDS             6104..7423
                     /locus_tag="VVE35_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU3061313.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFCGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7631..7703)
                     /locus_tag="VVE35_001112"
     tRNA            complement(7631..7703)
                     /locus_tag="VVE35_001112"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7668..7670),aa:Lys,seq:ctt)
     gene            complement(7968..9239)
                     /locus_tag="VVE35_001113"
     CDS             complement(7968..9239)
                     /locus_tag="VVE35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU3061314.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFNFPIELDEDALDFIATSTNGDLRSAFNSLDLAALSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9509..9748
                     /locus_tag="VVE35_001114"
     CDS             9509..9748
                     /locus_tag="VVE35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061315.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            9913..10092
                     /locus_tag="VVE35_001115"
     CDS             9913..10092
                     /locus_tag="VVE35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061316.1"
                     /translation="MNLIFEEHQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10229..10408
                     /locus_tag="VVE35_001116"
     CDS             10229..10408
                     /locus_tag="VVE35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU3061317.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGK
                     LNKYTERGIRKQAGL"
     gene            10445..10897
                     /locus_tag="VVE35_001117"
     CDS             10445..10897
                     /locus_tag="VVE35_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU3061318.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11188..11658
                     /locus_tag="VVE35_001118"
     CDS             11188..11658
                     /locus_tag="VVE35_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU3061319.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11677..12765
                     /locus_tag="VVE35_001119"
     CDS             11677..12765
                     /locus_tag="VVE35_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU3061320.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTANLKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            12746..13174
                     /locus_tag="VVE35_001120"
     CDS             12746..13174
                     /locus_tag="VVE35_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3061321.1"
                     /translation="MELEISDFPGCKIALFCWDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13310..14260
                     /locus_tag="VVE35_001121"
     CDS             13310..14260
                     /locus_tag="VVE35_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU3061322.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            14262..15005
                     /locus_tag="VVE35_001122"
     CDS             14262..15005
                     /locus_tag="VVE35_001122"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU3061323.1"
                     /translation="MQQYFVKGSAISPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDG
                     IKRLARVLDVEARQFELVQELDDNVELPVQVTISSGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15016..16812
                     /gene="pepF"
                     /locus_tag="VVE35_001123"
     CDS             15016..16812
                     /gene="pepF"
                     /locus_tag="VVE35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU3061324.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(16872..17549)
                     /locus_tag="VVE35_001124"
     CDS             complement(16872..17549)
                     /locus_tag="VVE35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU3061325.1"
                     /translation="MKPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWEI"
BASE COUNT         5444 a         2941 c         3845 g         5412 t
ORIGIN      
        1 cataggcaat tagaattatt agaattatta tttgaacata aacgttggtt tcatcgttct
       61 gaactagcag agttattaaa ttgtacagaa cgtgcagtca aagatgatct atcccatgtt
      121 aaatctgctt ttcctgactt gatttttcat tcttctacta atggtatacg cattattaat
      181 accgatgata gtgatattga aatggtttac catcatttct ttaaacattc aactcatttt
      241 tcgattttag aattcatctt ctttaatgaa ggttgtcaag ctgagagtat ttgtaaagaa
      301 ttttatatca gttcatcttc gctctatcgt attattagcc aaatcaataa agtgattaaa
      361 aggcaatttc aatttgaagt cagtctgacc cctgttcaaa tcattggaaa cgagagagac
      421 attcgttact tttttgcaca atatttttca gaaaaatatt atttcctaga atggccgttt
      481 gaaaattttt catcagagcc actatctcaa ttgttagaat tggtttataa ggaaacaagc
      541 tttccaatga atttgtcaac gcatagaatg ctaaagttgc tcctagttac gaacctatat
      601 agaataaagt ttggtcattt tatggaagta gataaagatt cttttaacga ccaaagtttg
      661 gattttttaa tgcaggcaga aggaatagaa ggtgttgctc agagttttga atcagaatac
      721 aatatctctt tagatgaaga agttgtttgc caattatttg tgtcttactt tcaaaaaatg
      781 tttttcatag atgaaagtct ctttatgaaa tgcgtaaaaa aggatagtta tgttgaaaaa
      841 tcttaccatc tattgagtga ttttattgat cagatttcag tcaagtatca gattgaaatt
      901 gagaataagg ataatctgat ttggcatctg cataataccg cacatctgta tcgtcaagag
      961 ttgtttactg agtttatttt atttgatcaa aaagggaata caatcaggaa ctttcaaaat
     1021 atttttccaa aatttgtttc agatgtaaaa aaagagcttt ctcactattt agagactctt
     1081 gaggtttgtt ctagttcaat gatggtaaat catctatctt ataccttcgt cactcatacc
     1141 aaacatttgg tgattaattt gctacaaaat cagcctaaac taaaggtttt ggttatgagt
     1201 aattttgatc agtatcatgc aaaattcgtt gcagagacac tttcttatta ctgtagcaat
     1261 aattttgaac tggaagtttg gactgaatta gaattatcaa aggaatcttt agaagattca
     1321 ccttatgata tcattatttc taattttatt attcctccta ttgaaaataa gagactcatc
     1381 tattcaaata atataaacac ggtctcactc atatacttgt taaatgccat gatgtttatt
     1441 cgattagatg agtaaccaaa aataacaaaa aaacgctatt tcgcgtttga actgtacctc
     1501 aaaagttaga cagaaaaagt ctaattttta gggtgttttt gttaagaaaa gaccttcaat
     1561 catttaagaa aaatgtacat ctagatttat agccctctaa aactcttgct ttttttgtag
     1621 aagatacgaa aaaagccata aaaccaaggc ttttcctgtt ttatgtagat gtttacacat
     1681 gaaaattgaa gtattttcaa aagctaatta gagtgatttt attcatgatg ggataaatga
     1741 tctggccaaa aatgattgag aaatagttgc ttaaaaagca tttgcatgat actatatatt
     1801 gaaaggaggt gataatatgg tagaacaaag aaaatcaatt accatgaaag atgttgcttt
     1861 agaagcagga gttagtgttg gaactgtttc acgtgtaatt aataaagaaa aaggcattaa
     1921 agaagtaact ttaaggaaag ttcaaagggc tataaaaaca ttggactata tccctgataa
     1981 ttatgctcga ggtatgaaaa agaatagaac agaaactatc gctttgatta ttccgagcat
     2041 atggcatcca tttttttcgg agtttgccta tcatgttgag aaagagttat taaaatataa
     2101 ttataaatta tttatttgta acgctgatag cgattcacaa aatgaaatcg aatatatcga
     2161 aatgttgaaa caaaataaag tggatggaat tattggtatt acatattctg atattgacaa
     2221 atatatttta tctgatttac cgtttgtaag tatagataga catttttcgg aaaatgtatg
     2281 ctatgttact tcagcaaatt acaaaggagg acaaactgca gcagaggaac tggttagaag
     2341 ggataataag caccttgctt ttattggttc aattaatatg tatgagaatg agactatact
     2401 cagaagtaaa ggattcagag aaaaagctaa ggaattagga gtagatgtct ataattttga
     2461 aatgaaagaa cctttaataa atcctgaaga tcagataaga atttttttgc aatctcataa
     2521 agaaattgat ggtatattta caataaatga tatgatggct ctgagagtta ttagtgttat
     2581 gaaagaactg gggaaaatag cacctgatga ttaccagata attgggtttg atggtttaaa
     2641 aacttctgtc gatcaatctt atctagtgtc tacaatagtt caagatattg gtatgatggc
     2701 aaaagaatct gttgcaatga tactgaaaat gattaagcga gaagagattc tagaaaaacg
     2761 aatcgtaatt cccacatatt tttggggaga taaaacaaca aaaaaaattt aaaaagcgct
     2821 tgacaattaa tgaaagcgct gttataatat aatcaacaaa tgaaacgttt caaaaatagg
     2881 ctagaagctt gtttaaaaaa taaataaaaa atgaaacgtt tcaaaaagga gggaaatgat
     2941 gcgtaaaggt acaatgttat ctgttgttgc ggggctatcg ttagctatac ttgcaggttg
     3001 tagtggagga actaactcaa aacaagcgag ttcttcaaat gatattaaag tctgggtaca
     3061 attctctgac gaaacaaatg aaggaaaagc ttggcaaaaa gttgttgata actttaatca
     3121 atcaggaaaa agtaagtaca aggttgtaac tgagtatatt cctcgtagtg gtagtggtgg
     3181 aggatatgaa gacaaggtta atgctgctat tacgactaat agtcttccag atgttattac
     3241 ccttgatgga ccaaatacgg cagcttatgc aaaatctaaa gtaattactc cgctagatga
     3301 ttatctaaaa gataataata tggatgatgt tttggacagt attaaacaac agggaactta
     3361 taatgggaaa ttctatgcat ttggattttc tgaatctaat gttggtgttt attacaataa
     3421 aaaaatgttc aaagaggccg gaattgcgga atcagagctt ccaacgctag aaaagccatg
     3481 gacatgggac gaatttaata cgattgctaa aaaattgaaa gatcattata acaaaccagc
     3541 aattgatttt cgtattaatt caaacgatga aatgttgcca tatgcttata tgccgttgat
     3601 ttggtcaaat aatggttctg tcgtaaacga agatggaaca aaggcagaag gatacttcaa
     3661 ctccaaagag agtgtagaag cagttcaatt cattcaaaat cttgtaaaag aaggctatac
     3721 aactgttagt ccagtcgaaa aaggttttga aacaggagag tatccaatgc ttttgagtgg
     3781 ttcttggacg attgctgaca tgaatacaaa ctacaaagat attgattttg gtatccttcc
     3841 ttacccagta tctagtaaga cgaaaaaatt agtatcccct tcaggtagtt ggcaattagc
     3901 tgtgacgacc aaatctgata aaaaagaggc cgcatctgaa tttgttaaat ttgctacaaa
     3961 tacagaatca agtgaaatta ttagcttggg gaattcggtc cttccaattc gtaaatctac
     4021 aattgaaaat atcaaagata aagtatccga accaatgcgt ttcttgatgg aacaaaattc
     4081 aaaaacagct catgctcgcc cagttgtcgt agcatatcct caggtttctc gagcttttca
     4141 acaagcaatg caagatatta gctattatga agaaaatcca aatgtgcaaa aagtcttgga
     4201 tactcgtaca aaagaaatgc aaactgctat tgatcagtca ctcaaataat ctgtaaaaaa
     4261 gaaggtggaa ggaaaatgtt tggaactacc ttcccctttc ttgttaatag cctagtttga
     4321 tatatatgat taaaggagat ggatatgagt aaagaaactg ttgtggatac aaaaagacgt
     4381 gaaaaaataa aagataacat actaggttat agttttttag ctccagcttt aattttatta
     4441 ggtatctttt tggtaattcc agttggaatg gtcatttatt acgcttttac agattattat
     4501 ttgttgacac cagatgaaag aaaatttgtt ggatttgaga attttattcg attaacaaaa
     4561 gatccgattt tcttaaagag cttcttgaat actttgaaat ttgttgtttg gattattcca
     4621 gttcaattgg gagcggcctt aggaatggct ttgattgtca ataaaaagcg aaaaggaaat
     4681 atgtttttta aagtggcttt ttttgctcca gttgtcatgt ccttggtagt tatttctatt
     4741 ctttggcttt atcttttaaa tccaaatagt ggcttattaa atgctatttt aaataaggta
     4801 ggtattgctt ctcaaccttt cttgactagt cctaaacaag ctatgtatgc aattgttttt
     4861 gtttctgctt ggcagggtgc aggttatcag atgcttttat ttttaggagg aatgcagaat
     4921 attccacaag atgtctatga agctgctgaa ttagatggat tctcaaaatg ggctcagttt
     4981 cgttatatca cgatgcccct actaaaacca acagccctgt ttgtattgtt aacaacttta
     5041 atttctgctt ttaagctgat tgtacaacca atggttatga cgcaaggtgg gccattaaac
     5101 tcaacaatta ctatggtgta ctatatttat caacaaggtt ttactgatcg tcttgtagga
     5161 tattctagct caatcgcttt agtatttaca acgttaattg gtatgattag cttagtacaa
     5221 cgtcgcgttc tgaaggagga tgactaagat gaaacgattt aaaccaacaa caatcttaga
     5281 atatattctg ttaactttat tggcaggact cttccttttt cctatggttt ggatggtagt
     5341 ttcagctatg aagccagaag cggatgttta taaaaatttg actagttggc aagccttctt
     5401 accaagttta aatcccgctg attggtttaa accttatcag gaagtatttg agcgctttag
     5461 tatttttaca tatctaggga acagcatttt ttatgctgga acgtttgcta ttggttctat
     5521 tattgtcaat gcactggccg gtttcgcttt tgcaaaagtc aatttttcag gtaagaaaat
     5581 tttatttggt ttcttacttg ctttacttat tattccagtg gaaactgtgt taattccaca
     5641 atttaccatt atcaattcct taggtttggt caataatcgt tttgcagtta ttattccagg
     5701 attggcgagt gtatttaata tttacttatt taggaatttc tttattgcaa ttccagaaga
     5761 gattcttgag tctgcaaaga tggacggagc aagtatcgtt agaattttct ttagaattat
     5821 gcttccaatg tctaaacctg cagttgctac agttggtgtt ttatcattta tttcaagctg
     5881 gaatgattat atctggccat tgatggtttt gacagattct tctaaatttt caatgcaggt
     5941 tgcaattaca actattaata caactcaacc agtttatatc aatcaggtaa tggctgtatt
     6001 aactatttca acgattccgt tgattttggt ctatgtagtc gctcagaaat atattgttca
     6061 aggtctgggt ggttccggaa ctggaataaa ataggatata cttatgataa ataaaacatt
     6121 tacagtagaa aaagccaatc gttttatagc agaaaataaa catctcgtta atactcaata
     6181 taagcctgaa gaacattttt cagctgagat tggttggatc aatgatccaa atggatttgt
     6241 ttatttttgt ggagaatacc atctctttta tcaattctat ccatatgata gtgtttgggg
     6301 acctatgcac tggggacatg ctaaaagtaa ggacttggtg acttgggagc acttgccagt
     6361 ggcacttgct cctgatcaag attatgaccg aaatggttgt ttctcaggct ctgccattgt
     6421 caaggatgat cgcctctggc tcatgtacac tgggcatatc gaagaaaaaa ccggtgtccg
     6481 ccaagtgcaa aatatggcat tttcagatga cgggattcac tttgaaaaga tttcccaaaa
     6541 tccagttgta actggaacag acttgccaga tgagttgatt gctgctgatt tccgtgatcc
     6601 aaaactcttt gaaaaagatg gacgctatta ctctgtagta gctgccaaac acaaggataa
     6661 tgtgggctgt atcgttctac tagggtccga taacctagta gaatggcagt tcgaatccat
     6721 ctttttaaaa gggggagaac accaaggctt tatgtgggaa tgcccagatt acttcgagtt
     6781 agatgggaaa gattgcctta ttatgtcacc catgcgttat cagcgtgagg gagactcata
     6841 tcataacatc aactcatcgc ttttgttcac gggtaaggta gattggggag aaaaacgttt
     6901 tatcccagaa tcagttcaag aaattgatca tggccaagac ttctatgcgc ctcaaacatt
     6961 gttggacaat caaaatcgtc gtatcctgat tgcttggatg cagacatggg ggcgtaccct
     7021 tccaacccat gaccaagaac acaaatgggc atgtgccatg actttaccta gaattctaag
     7081 attggaagat ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc aaatccaaat
     7141 agataaagat tgtcattacc acttaggaaa tgatatagat tatcttgaat ttggttatga
     7201 cagtaatgcg cagcaagttt acattgatcg tagccatctt attcaaaaaa ttctaggtga
     7261 agaagaacag gacactagtc gacggtatgt agatattgaa gctaaagaat tggaagttgt
     7321 tctagataaa aattccatcg agatttttgt caatcaaggt gaagcaagct tgactgcaac
     7381 ttattactta acggtgccag ctgagctatc acgaattgat taaaaattaa gttatttctc
     7441 ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga ctttttgtgt tttgggtata
     7501 taagcttagt ttatggtatt tgtaaaattg gtgttggatt atgatttaag ctagttttct
     7561 aaagaatttg aaaaaaattt tatttaagca aaaaaacctt ggttccaagg cttttcctgt
     7621 tgtatttaga tgccccctac agggatcgaa cctgtgaccc acggattaag agtccgctgc
     7681 tctgccagct gagctaagga ggcaaagaaa aaagctgtat tggtgccgaa acttcacggt
     7741 ttgtattgaa cccgcgcaat taagcaggtg ggcaactcgc tctaactgaa gctgtttccg
     7801 tgtgagacgg cctacatgct gttagaagac ttttgtttcc ctaataatac aaaaaatagt
     7861 cggtcaacac ttaagtgtga agtcgtacac cacagcgttt ctatacttat atgataccac
     7921 tttttcaaaa aaaatcaaga ggaaagtgca attttttgaa aaggatttca gatttttcgc
     7981 aaacggtcga tagcttcctt tctttgagcc aaagcccgtt catatttacc agtatcttct
     8041 gcttggaaat agtgatgatt acgaattttt tctggcagat agtcttgctt gacccaattt
     8101 ccaggatagt tgtgtggata gagatagtct tgggcattcc ccagttcctt gcttccactg
     8161 tagtgcccat cacgtaggtg tcgcggaata ggcaagtgcc ctgatgtttt gaggtcagca
     8221 agtgccttat ccatagctac ataggctgag ttggattttg gagaaagtgc caaatcaatc
     8281 acgacattgg caatgagaat gcgggcttct gggaacccaa tcttctgggc agcatccaga
     8341 gcagtcacgg tatgaatctg ggcctcagga ttggctaagc cgatatcttc ataggcgata
     8401 acagtcaagc gacgagcgag actaggcaga tccccagcct caatcaagcg ggcagcatag
     8461 tggagactgg catccacatc tgagccacgg atagactttt gcagggcaga tagaacatca
     8521 tagtgaccgt ctccatcctt gtccatagtg atgtagcttc tctgaagact attttccatg
     8581 atgtctaagg tgatgtggcg aatgccctcg tcattctcag gggtagagag agcagccaag
     8641 tccagtgagt taaaggcaga gcgaaggtct ccgtttgtag aggttgcgat gaaatccagc
     8701 gcatcctcat ctagttctat tggaaaatta aaaccacgtt cagggttact tagagctatc
     8761 tgcagggcct ctttgacgtc ttggttagac agaggttcca actcaaaaat ttgaacacgg
     8821 ctacgaatgg caggagtgac ggaaaagaaa ggattttcag tcgttgctcc aatcatgatg
     8881 accagtccac tttccaagag aggcaagagg aagtcttgct tggtcttatc tagtcgatga
     8941 atttcgtcta gcaataggac gagaccacca gaaaatttag cttcttccga gatttcttgc
     9001 agtcgctttt tactatcaac tgtcgcattg aaagttcgaa aggcatactt ggtcgttcca
     9061 gcgatggcag aggcaatact ggttttgccg attccaggag ggccatatag aatcatggag
     9121 gacaggcggt tggcttccac catgcggcgg atgatttttc caggtccgac cagatgctcc
     9181 tgaccgatga cctggtcgat ggttttaggg cgcatgcgaa gcgcgagatt gtctggcata
     9241 gcagtccttt ctaacgtgga tttgagtaga atgactcatc tactatatct tactatgtcc
     9301 attctagcct attagttgtg atatttcaat ggttttgtaa taatcaggga gtgagataga
     9361 atctttttta ctatttaatt taatgaggag aggtatcagc atgagagatt tgattttcct
     9421 taaagctcaa aataggatag ttgagcctaa agaagtggaa gtgctgactt gagaaataat
     9481 attgctaaat gtcattgtat gaactataat gaaaagagag gtgataagca atggaaatga
     9541 tggaccaagt caggaaattt tgatttttac aaaacaaatt cgtcattgga ttcttagtga
     9601 tcaagttatt tcagggaaaa gaaaactttt cttccgggaa gatactccaa aagagatttt
     9661 agatttgtac gaaaacatta aatctaaact tgattttgct taccaagaag ttcattctaa
     9721 taatgggttg aaaaaatatg aaaaatagaa taattgatgt ttttgaagta gtaaatcgcc
     9781 tccttgtaat aactgttgaa aatcctgatt tcgaagactt gagagttaat catactcaat
     9841 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgt acttgagtac ggtaaggcga
     9901 cgttgacgtg gtttgaattt gattttcgaa gagcatcagt ttgtaaaaat tggagataaa
     9961 aaatatagag tacgcagtgt tcctatgatt cattcaactc cgcctcaatc tgtcttagat
    10021 cgagatactt ttacaattga ctatacagat gatgaattgc tcgataaaga agcagttttt
    10081 actactcatt agaaaaatag gtatgaataa attggcttcc cttagaacca catctagggg
    10141 cttttatgta taatagaata ataagtatca ctcctttaca atacgtgtaa cacgtgttgt
    10201 aatggtgttg tcaggaggta aaagcgctat gcctatgaca caaaaagaga tggttaaact
    10261 ccttacggcc catggttgga taaagactag aggcggtaaa ggctcccata ttaaaatgga
    10321 aaagcaaggg gaaagaccta tcacaatcct tcatggtaag ctgaataagt acactgaaag
    10381 agggataaga aagcaagctg ggttgtaaaa ttcagcccct gctttttgat atggaggtag
    10441 tgaaatgtta gttacgtatc cagctttatt ttattatgac gacacagacg gaacagaagc
    10501 gacttatttt gtccatttcc cagattttga atactcagct acacaaggag aggggatttc
    10561 tgaggctttg gctatggggt cggagtggct agggataact gttgcagatt tgatagagag
    10621 tgatggagag ttacctcagc catcagatat caatagcttg tctttaattg ataatgatcc
    10681 ttttaaagat gatgaagatt tcgtgtcaac ctatgacctt gataaatctt ttatttctat
    10741 ggtatctgtt gatgtatcag aatacctagg aagtcaggaa cccattaaaa agactttgac
    10801 cataccaaaa tgggcagata agttgggacg agaaatggga cttaactttt ctcagacttt
    10861 gacagacgct attgcagata agaaagttca agcctaagat taagcgtggt atcgtggtaa
    10921 tagtcaataa aaaagcacgt ccacttgtgc tagttactcg cctattgatt ttatagattt
    10981 attagccctt tcgcgggctt tttatattga tttttatgag aaatgaagaa atcagacttt
    11041 taagaaaatc attgatacca agggtttaaa tgaggtaata tggtataatt aggacataaa
    11101 ataattttgt ggtaagatgg tagtatctat tttagcatat ttccgagcaa tcggggcgat
    11161 taaagagtcg catagaaaga ggacaaaatg gcaacatacg gatttttaga tattttagag
    11221 gaagagttgg acaagaactt tccctttgac tttgagatta gttgggacaa gcgtaaccat
    11281 gcggtagaag tgagttttct attggaagcg caaaatgctg caggtgtgga gatggtggat
    11341 gaagacggag aggtttcatc agatgacatt ctttttgagg aagcggtcct tttctacaat
    11401 cctgccaagt caacagtcaa tgaggaagac tatttgactg ttattcctta cctgcctaaa
    11461 aaaggatttt ctcgcgagtt tttagcttat tttgcccttt tcctcaaaga tactgcggaa
    11521 gttgggctag ataccctcat ggactttttg gaagacccag aagcagagga atttgttatg
    11581 gaatggaatc aagaagtctt tgaagaagga aaagttggct tggaagaggg agaattttat
    11641 ccttatccaa gatactagga gttggttgga ggtgttatga agaaaattgg gacttatttg
    11701 gtttatgtgc tagctgttgt ctttattatg ctagcttttg cttgtggaac aatcgcattt
    11761 gcagagttgg ggtattccgc agttttagcc tttacttttg gttatgcctt cgctcttcta
    11821 agcatgtatt taatctttat tcttcatgag ctgggtcatg ctttttgtgg ctacttgaca
    11881 ggctatcggc tggtggcttt tggattagga cattttattt tgaccaaaaa gtcaggcagg
    11941 tttcatctta gtagaacagc caatctgaaa aatgttggtg ctcaatacat tggtttaaaa
    12001 gaggatgaaa gcgatcaaag aatcatcctg atgctttcag gaggcttgat ggttcatctc
    12061 ggcttgatat tattggcgat agtgtttgga tttttgacaa gaagctggta ttttgcaggg
    12121 acttggattt ttcttaattt atctttcttc ctaaataaca ttttgccagt cgacatcacc
    12181 gatggagcaa aaatttggga attactacaa caccctgaaa ataccaaata cgcctacctg
    12241 gtattgagac attctgctca aactttgctg gctcctcaag aatatgattt gaaagacttt
    12301 gttcaagcag ttcctgagga tgcacgaggg agttttgcgg atggtgtatt aggtatgcaa
    12361 ggtgaagttt ccattttgga agggaaggaa gaagtagcta aacagcagtt tcaagcatta
    12421 cttgagagga caactactcc gatgatgcag acagttgccc aactatccct gcttcatatc
    12481 gccttgttgg aaggagattt tgagcaagcg gagcaatatg ctagtattcg acaggtcaaa
    12541 tcgtttctgt ctctcaaact gtccaatctt caagtgatac aagcctggta tcaattaaag
    12601 gtgaagaaag atgtagatcg aactcgtaag gctctcaaga ttgctagaca gaaaatggat
    12661 agtagtcgaa tgttgaggga cgagaaacgc tattatgaaa acttgttagc cgagctggaa
    12721 aaggaaatgt tagaaggaac tgaagatgga actagaaatt tctgatttcc caggttgcaa
    12781 gattgctttg ttttgttggg ataagctttt gactatctta cgcgatgata aggcaagtat
    12841 tccctgggcc aatatgtggg aactgccagg tggtggacgt gaaggtgatg aaagtccttt
    12901 tgagtgtgta gcgcgtgaag tttatgaaga actgggcatt catctgactg aagattgtct
    12961 gctttggagt aaggtttacc caagtatgct ttttgcagac aaacagtccg tttttctagt
    13021 tggtcagttg acacagaacc agtttgacag tatcgtattt ggagatgaag gacagggcta
    13081 tcagctgatg aatgttgagg aatttcttag ttccagtcaa gttgtgcctc agttgcaaga
    13141 gagattaaaa gattatttaa aagtaagtga ttagaaagga tggttcggtt aaatttctaa
    13201 actgaacccg ccctaaacac ggtgccaaaa agataaactt ctcttagaca caaacgtctt
    13261 cagagagttt cctattttgg ctttgtgttt tacgggcttg gtattaataa tggaaacatg
    13321 gcaagagtta aaagttacag tgaagcgtga gggagaggag ttggtttcca atctcttgat
    13381 tgagctggga gcgcaagggg tagcaattga agacagcatg gactatgtgg gaaatgttga
    13441 ccgctttggc gagattttcc cagaggtgga gcagcaagaa gaaatcgtag tgacagccta
    13501 ctaccctgac acggtggatg tgacagtggt tgaggcggac ttgcaggctc gtctagcaga
    13561 attgacagat tttatggatt tgggagagtt aaaaataggt acgactgcct tggctgagga
    13621 agactgggca gacaactgga agaaatacta tgaacctgct cgcatcactc atgatttgac
    13681 catcgttccc tcttggacag actatgaggc gactgcggga gaaaaaatta tcaagctgga
    13741 tcctggcatg gcttttggta cgggaaccca cccaactacc aagatgagcc tttttgcctt
    13801 ggaacaggtt cttcgtggtg gcgaaacggt gctagatgtg ggaactggtt caggggttct
    13861 ctctattgcc agctcgcttc tgggggctaa agaaattttc gcctatgacc tggatgatgt
    13921 ggcagttcgt gtggctcagg aaaatattga gctcaaccct ggaatggaaa atatccatgt
    13981 agcggcaggc gatttgctta aaggcgtgga gattgaagca gatgtcatcg tggcaaacat
    14041 cttggcggat atcctcattc atctgacaga ggatgcttat cgcttggtta aggatgaagg
    14101 ttacctgatc atgagtggca ttatcaagga taagtgggac atggtgcgcg agttggctga
    14161 atcagctgga tttttcctcg aaactcacat ggttcaaggg gaatggaatg cctgtgtctt
    14221 taagaaaacc aaggatatct ctggtgtgat tggaggctag catgcagcag tattttgtaa
    14281 aaggcagtgc tatctctcct gtcactatcg aggacaggga aaccagcaag catatgtttc
    14341 aggttatgcg cttgaaagaa gatgatgagg ttactttggt ctttgatgat ggaattaagc
    14401 gcttggcgcg tgtgctggat gtggaagctc gtcagtttga attggtccaa gaactagatg
    14461 acaatgtgga actgcccgtt caagtgacca tttcatctgg ttttcccaag ggagataagt
    14521 tggagtttat cactcaaaag gtgacggaac taggtgccag ccagatttgg gccttccctg
    14581 ccgattggtc cgttgctaag tgggatggca agaaattggg taaaaaggtt gaaaaactag
    14641 aaaaaattgc ccttggagca gccgagcaaa gcaagcgtaa tattgtacca agtattaaac
    14701 ttttcgagaa aaaagcagat tttctagctc agttggacca gtttgactct atcatagtag
    14761 cctatgaaga atcagctaaa gaaggagaag ccgctgctct tataaaagca gttactggac
    14821 tcgagaaagg agccaaattg ctctttatct ttggtccaga aggcggtctc tcacctgcag
    14881 aaatcgaaag ttttgaagct aaaggagccg ttttggcagg cctaggtcct cgtattttgc
    14941 gagcagaaac agcaccgctt tacgctttat cagcccttag tgttttagta gaattagaga
    15001 aataagagga agaaaatgga acaaaaacac cgttcagaat ttccagagaa ggaactctgg
    15061 gacttaacag ccctatacca agaccgtgag gatttcttgc gtgcaatcga gaaagctcgc
    15121 gaagacatca accagtttag ccgtgattac aagggcaatc ttcacacttt tgaggatttc
    15181 gagaaggcct ttgcggaatt ggaacagatc tacattcaga tgagccatat tggcaactat
    15241 ggttttatgc ctcagacgac ggactatagc aatgacgaat ttgccaatat tgcccaagct
    15301 gggatggaat ttgaaacagc tgccagcgta gccttgacct tctttgacga tgccttggtg
    15361 gcagcagacg aggaagtctt ggaccgtttg ggtaaattgc cacatttaac agctgccatt
    15421 cgtcaggcta aaatcaaaaa agcccactac ttaggggcag atgtggagaa ggccttgaca
    15481 aatctcggtg aagtttttta cagtccgcag gacatttata ctaagatgcg agctggggat
    15541 tttgaaatgg ctgactttga agcccatggc aagacctaca aaaacagctt tgtgacctat
    15601 gagaatttct accaaaatca cgaggatgct gaggttcgtg agaaatcctt ccgttccttc
    15661 tcagagggac ttcgtaagca ccaaaatacg gctgcagcag cctatctggc tcaggtcaag
    15721 tctgaaaaac tcttggctga tatgaaggga tacgactctg tctttgacta tcttctagct
    15781 gaacaagaag tggaccgtgt catgtttgac cgccagattg acctcatcat gaaggacttt
    15841 gcaccagtcg ctcagagata cctcaagcat gttgccaagg taaatggtct tgaaaagatg
    15901 acctttgcag actggaaatt ggacttggac agcgccctga atcctgaagt gactattgac
    15961 gatgcctatg atttggtcat gaagtcggta gaacctttgg ggcaagaata ttgtcaggaa
    16021 gttgctcgtt accaagaaga gcgctgggtg gactttgctg ctaacagtgg caaggattcc
    16081 ggtggttatg cggcggaccc atatcgcgta cacccttatg tactcatgag ctggacaggc
    16141 cgtttgagcg atgtctatac cttgattcat gaaatcgggc attctggtca attcatcttt
    16201 tcagacaatc atcaaagcta tttcaacgcc cacatgtcga cctattatgt tgaagcaccg
    16261 tcaaccttca atgaattgct actcagtgat tacttagaga accagtctaa tgacccacgt
    16321 caaaaacgct tcgctctggc tcatcgcttg acagacacct acttccataa tttcatcacc
    16381 cacctcttgg aagccgcctt ccagcgtaag gtgtatacat tgattgaaga aggggagacc
    16441 tttggagcaa gcaagctcaa cagcattatg aaggaagttt tgacggattt ctggggagat
    16501 gctattgaaa ttgatgatga tgcagctctg acttggatgc gccaagctca ctactatatg
    16561 ggcttgtata gttacactta ctctgctggt cttgtcatct cgacagctgg ttaccttcat
    16621 cttaaaaact cagaaactgg agctgaagac tggctcaatc tcctcaaatc aggtggtagc
    16681 aagacaccac ttgagtcagc catgattatc ggtgctgata tttcaacaga caaaccactc
    16741 cgtgatacca tccaattctt gtctgacaca gttgaccaga ttatcgccta cagtgctgag
    16801 ttgggggagt aaggggatag agaggttgtt atagaatggg tatggacagt tatttgctta
    16861 aaagtccatg tttaaatctc ccattccttt tcgaggaatt ctttgccctt ctctgtaatg
    16921 gtaagtgaac gtggcgtttg tgcagggatg accaattgtt tttcactgaa aaattctaaa
    16981 agagcattgc cgagagctcc gccgacatga tttttacgtt cgctccaatc caaacattta
    17041 gggcaaaatt tccgtttttt ctttttcaat tcatccgtat caatgtgtaa ttttctttgg
    17101 aaataagact tcccagcttc agtgagttgg aagtcttttt ctgattcgct gaggagattt
    17161 tcttgcaaga gataattggt aatggaaatg ccaagctcac cagcaatatg gtcatagcag
    17221 gtgcgagcga cctttaattc ctttttgtga atgtgttgtt tataactttt gacatcacgc
    17281 tgaggagagt agaagctgag aaattccagc atttctgcaa tggcagcact ttttaaagaa
    17341 taataatgaa aacgtccata ggtttgtaga gtggttacct gcgcttcggt aagtaatttg
    17401 aggtgatagc tgacagtatg ttgcttgatt ttggtaaagc tagcaatttc gttgaccgtg
    17461 tgagcttttc catccattag aatatctaaa atgtgcatcc gagaagggtc tgataagagt
    17521 tttgtgatga tgaaaatatc tggtttcata aaaattttct ccaaatttca tatatcgatg
    17581 attttctatc ttttattata ctataattaa agcacaagaa aaatagattt taaaaatgtt
    17641 tt
//
LOCUS       DAVGJJ010000036        17013 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000036 DAVGJJ010000000
VERSION     DAVGJJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.036"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(252..1886)
                     /locus_tag="VVE35_001125"
     CDS             complement(252..1886)
                     /locus_tag="VVE35_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061326.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1867..2616)
                     /locus_tag="VVE35_001126"
     CDS             complement(1867..2616)
                     /locus_tag="VVE35_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061327.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMIKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGNLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2795..4249
                     /locus_tag="VVE35_001127"
     CDS             2795..4249
                     /locus_tag="VVE35_001127"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU3061328.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4339..6957)
                     /gene="alaS"
                     /locus_tag="VVE35_001128"
     CDS             complement(4339..6957)
                     /gene="alaS"
                     /locus_tag="VVE35_001128"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU3061329.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNCRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVSHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(6979..7464)
                     /locus_tag="VVE35_001129"
     CDS             complement(6979..7464)
                     /locus_tag="VVE35_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU3061330.1"
                     /translation="MKIFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKISKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYAGLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7739..7978)
                     /locus_tag="VVE35_001130"
     CDS             complement(7739..7978)
                     /locus_tag="VVE35_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061331.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8239..9309)
                     /locus_tag="VVE35_001131"
     CDS             complement(8239..9309)
                     /locus_tag="VVE35_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU3061332.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGMENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9306..10079)
                     /locus_tag="VVE35_001132"
     CDS             complement(9306..10079)
                     /locus_tag="VVE35_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061333.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10076..10882)
                     /locus_tag="VVE35_001133"
     CDS             complement(10076..10882)
                     /locus_tag="VVE35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061334.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10863..12020)
                     /locus_tag="VVE35_001134"
     CDS             complement(10863..12020)
                     /locus_tag="VVE35_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061335.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDDVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12181..13086)
                     /gene="murB"
                     /locus_tag="VVE35_001135"
     CDS             complement(12181..13086)
                     /gene="murB"
                     /locus_tag="VVE35_001135"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU3061336.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13198..13950)
                     /locus_tag="VVE35_001136"
                     /pseudo
     CDS             complement(13198..13950)
                     /locus_tag="VVE35_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(13960..14691)
                     /gene="budA"
                     /locus_tag="VVE35_001137"
     CDS             complement(13960..14691)
                     /gene="budA"
                     /locus_tag="VVE35_001137"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU3061337.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGTGEQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14833..16104
                     /locus_tag="VVE35_001138"
     CDS             14833..16104
                     /locus_tag="VVE35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU3061338.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNTIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16124..16939)
                     /locus_tag="VVE35_001139"
     CDS             complement(16124..16939)
                     /locus_tag="VVE35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU3061339.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT         5150 a         3831 c         3306 g         4726 t
ORIGIN      
        1 tttcgaactc taaatatatg ttctatcaaa aatacttgga tcacacacat tataggaatt
       61 aacgtttttg aaattgaaaa atatcccaat aaataaacta taaacaacaa taatagaact
      121 atattatatt tcttattcaa aacattcctc cctatatttt tgattaccaa tcttaatcat
      181 ttacaactac attctaacaa actataaaag cgtttgtcga attgaattta tcaagcaagc
      241 aaccaactat ctcatctttt ttctatttct gccaatatgc gtgacaggta ataatgatag
      301 ccaaaaatag caagagcaag caagacgata agagctccta ctcccaagtt gatggcaagg
      361 ataggggaga gagactgaac caagaatatg ctcccaatta caagggccat caggattgca
      421 ctataaataa acaataaaac tatggcgact atgccatttg aacgattcac caggtccgta
      481 atgctactcc aatttgttga cagattttta acgtccttaa agtaatggtg gcaagaaagg
      541 atgacactgg caatgatcca gactacaaga aggtaaatca tcgaaaggat ggacaagcct
      601 agatatagag aaagaccgag caaagtcaga actggtaaaa aggactgaac agcaaagata
      661 atccaaaatt tcactttcac ataacgagca aagtcaaagg gtaaactctt cagaaaatca
      721 acattttccc tctccaagga caaggcaatt gaaggcaggc tggtgatatt gttattgaca
      781 actgctataa acactcctac aaaaaacaag ggtaaccagt atggaggatg aatgtctgga
      841 actatctgag aatctcggat tttggaaatc agactgatca tcatgagata aggaaggaaa
      901 gcacttgtaa aaagcactgt aatcacgcca gtcccctgtc ccaagagggt gaggtggtag
      961 cgtaaaacca tacggaaaaa gcccttttta gtagttgaaa tcctttcctt gctgcgacgt
     1021 tcttttttga ccttctcctc actgttaagc aggatcacgt cataaaaacg aggaaggacc
     1081 ttctttttgg tcaggtaaag caagaaaaca gttaaagcta tccaagcgag cagacccaat
     1141 atggcttctg tcgaaaaagg ctccactgct attttgtaaa agatatgaag aggataaagg
     1201 agaaatggaa tgtctctaac tttgtcaaca atacttccaa aagtcgactg aagaaagaag
     1261 ataaatatta aaggtatgag aactcctatc ccaatcatca cattcgaaaa aatagactga
     1321 tactttctga agaccgtagt ttgagccaag aaatgtactg ccactaccat cactaaagta
     1381 acagagacaa ataataaggt caaggacagt agcatcaaag gcaaacccaa ccacagagaa
     1441 ggtgctagcc taatgtagag gaccagaaaa taagctagga ttggtacaat tccagctaga
     1501 gctggcaaga ggacagacag acctttggcc attataatct ctgattcttt aaaggcatag
     1561 ggcctatagg aggccaggtc cttactctca taaaagacat tgtaaaatgc agtgaaagaa
     1621 gttgaaaagg ccagcaccag taaaatagca accatcgtac taaaaaaact tggcatttcc
     1681 tcaaaaggaa aacgaaaggc tatatttata aacatgaaaa gcatcaagag actagaaaaa
     1741 atgtaagaac ttaggactct agcggaaaca tttacttttt tcccaggatt cttagcctgc
     1801 ttctttcgta gattagccag attagcttct tgagatgaat agaggatatt gatatcaact
     1861 aattttttaa tgaccttgag acgcatctga aacctcctct tttctaccag caaggctaag
     1921 gtagatactt tccaaagact ggtctgggtt gtcttttctc aagtcctcta ccttaccaca
     1981 ataaatcaaa ttcccctttt tcaaaatagc aatccgatca cagacttgct cagctacctc
     2041 tagcacatga gttgaaaaca aaactgtctt acctttttgc gcatgctcct taatcatttg
     2101 cttcaaatca aaggcggcct ggggatccaa accagtcaag ggttcgtcca aaacccaaat
     2161 atcaggatca gacaagagtg ccccgatgac aaagactttc tgacgcattc catgagaaag
     2221 agtttcaata acctgatagc gattttcagc aaaatcaaaa acgttcaata gcctagctag
     2281 actagcctcc aagtcagatc tactcagatc ataggatgag gcgatcaatt cccaaaattc
     2341 attggccgtt aagcgtaaaa ataagtcagg cgagtctgct acgtagccaa tctttcgttt
     2401 aatagccaag cgattttccg ataactcctg accgtctacc aaaatacgac cactgctggg
     2461 tgaaatgata ctgactaggg attttatagt ggtcgatttt ccagccccat tatgaccaat
     2521 caagcccata atctctccat tttcaatctg caaattgaga ttactcaagg cctctttatc
     2581 accatacaac ttactaacat tttgaaattc aatcatggga caactcctat ctttatctgt
     2641 attatatatc gctatactag cactttgata caaaaaaatc aatactttat tttaagtagt
     2701 taaaatagtt tccttctatc ttacgcaaac gtttgcgcta atagatactt tatgatagaa
     2761 taaagaacaa gattgacaag taagaggaaa cattatgcaa aatcaaacac tcatgcaata
     2821 ctttgaatgg tatctgcccc acgacggtca acactggacg cgtctggctg aaaatgctcc
     2881 acacctagct catctgggga tcagtcacgt ctggatgcca ccagccttca aggcaaccaa
     2941 cgaaaaagat gtcggctatg gggtctatga cttatttgac ttaggagagt tcaaccaaaa
     3001 agggactgtc cgcaccaagt atggtttcaa agaagactat cttcaagcca ttcaagccct
     3061 taaagcgcag ggaattcaac ctatggccga tgtggttctc aaccacaagg ctgctgctga
     3121 tcacagggaa gcctttcaag ttattgaagt ggatcctgta gaccgtacag ttgaacttgg
     3181 agaacccttc accatcaatg gctggactag ttttaccttc gatggtcgcc aagataccta
     3241 caatgacttc cactggcact ggtaccactt cacaggtaca gactatgatg ccaaacgccg
     3301 taagtctggg atttatctga tccaagggga caacaagggc tgggctaacg aggaattggt
     3361 cgataacgaa aacggaaact acgactacct catgtatgcc gacctagact ttaaacatcc
     3421 tgaagtcatc caaaacatct atgactgggc tgattggttc atggaaacga ctggtgtagc
     3481 tggtttccgt ttggatgccg ttaagcatat tgactctttc tttatgcgca acttcatccg
     3541 cgatatgaag gaaaaatacg gtgacgattt ctatgttttt ggtgaatttt ggaacccaga
     3601 caaggaagcc aatctggact atctcgaaaa aacggaagaa cactttgacc ttgtcgatgt
     3661 tcgtctccat cagaatctct ttgaagccag tcaagctggc gcaaactatg accttcgtgg
     3721 cattttcaca gatagcctgg ttgaactcaa gcctgacaag gctgtgactt ttgtcgacaa
     3781 ccacgatacc caacgaggac aagcccttga gtctaccgtt gaagaatggt tcaagccagc
     3841 agcctatgcc ctcattttgt tacgccaaga cggccttcca tgtgtctttt acggagacta
     3901 ctatgggatt tcaggccagt atgctcaaga agatttcaaa gaaatccttg accgcctcct
     3961 agccatccga aaagatttgg cctatggaga acaaaatgac tactttgacc atgctaactg
     4021 tatcggttgg gtacgttcag gtgctgaaaa tcaatcccca atcgcagtcc ttatctcaaa
     4081 tgaccaagaa aacagcaagt caatgtttgt cggtcaagaa tggactaatc aaacctttgt
     4141 agatttactt ggtaaccacc aaggtcaagt tacaattgat gaggaaggtt atggacaatt
     4201 ccctgtctca gctagatccg taagtgtctg ggcagtcaat accatctaat agctcataat
     4261 aaccaagcta ggtccaagcg gatttggctt ttttgtattc acaaaaagac ctacccaaat
     4321 ggatagatct ttacttgatt acaatttacc tgctactgca tccaacaatt cttggatctt
     4381 aggttggttg cttcctcctg ccatggccat gtctggttta ccaccaccac gtccatcgat
     4441 gattggtgct aattctttga caaggtttcc tgcatgaagg tcttttgtct tgcttgctac
     4501 aaggacattg actttgtcac cgatagcggc aactaggaca agaagatcag agtagtcttt
     4561 ttgtttccag ttatctgcaa aggtacgaag ggcacctgca tcggatacag acacttgact
     4621 agcaatgtaa cgatgaccgt tgacttcctt aacatctttg aagatatcgc ctgcggctgc
     4681 agctgcggct ttttctttca actcagcatt ttctttttga agttgacgaa gttgttcttg
     4741 aagtccttct accttgtgag atacttcctt gacttgaggt gctttcaagg ttgctgcgac
     4801 agctttaaga gcatcctctt gttcacgata ggcttcaaag gcttccttac cagtcactgc
     4861 caagatacgg cgagttcctg aaccgattcc ttcttctttg acaattttga agagaccaat
     4921 ctcagaagtg ttgccaacat gagtaccacc acaaagttca atagagtagt caccaatagt
     4981 cacgacacga acttccttgc cgtatttctc accaaagagg gccatcgctc ccatttcttt
     5041 agcagtgtca atatccgttt caactgtctt aacttcaaga gcttcccaga ttttttcgtt
     5101 gacttgctgt tcaatcgcac gcaattcttc agcagttaca gcttggaagt gggtaaagtc
     5161 aaagcgaagg aattcaactt cgttaagaga tcctgcctgt gttgcgtggt ttccaaggat
     5221 attgtgaagg gcagcgtgaa gcaaatgagt cgcagtgtgg tttttcatga cacggtgacg
     5281 gcaattgcta tcaattgcca aggtatattc ttggttcaag gcaagcggtg caaggacttc
     5341 aactgtatga agggcttgac catttggggc tttctgaaca ttggtcacag tagccacaac
     5401 cttacctgac tcatccaaga tttgtccgtg gtcagctacc tgtccaccca tttcagcata
     5461 aaatgacgtt tccgcaaaga taagagaggc agttccttct gaaacagctc ctacttctgc
     5521 attgtcagca acgatagcta ccaatttaga agacaattgg ctagcattgt agttgaagac
     5581 actttctaca gtgatatttt gaagagtttc attttgcata cccattgagc cacccttgac
     5641 agctgacgca cgcgcgcgtt cttgctgttc tttcatggct gcttcaaaac cttcacggtc
     5701 tacagtcata ccagcttctt cagcaatttc ttcagtcaat tcaactggga atccgtatgt
     5761 atcatagagt ttaaagacat cttgaccagc aatgacagat tgaccttttt ctttcaagtc
     5821 tgccacaatg ccttgggcaa agtgttgacc tgagtgaagg gtacgagcaa atgactcttc
     5881 ctcgctctta acgatttttt caataaagtc acgtttttca agcacttctg ggtagtagct
     5941 ttccatgatt tttccaacag ttggaacgag tttgtaaagg aaaggctcgt tgatacccaa
     6001 tttttgacca tgcatagaag cacgacggag aagacgacga agaacataac cacgaccttc
     6061 atttcctgga agggcaccat caccgatagc aaatgaaaga gaacgaatgt ggtctgcgat
     6121 aaccttgaag ctcatgttgt cgccatcttg gtcataaacc ttaccagaca atttctcgac
     6181 ttcacggata atcggcatga agaggtccgt ttcaaagttg gtcttagccc cttggataac
     6241 agccaccaaa cgctccaaac cagcgcccgt atcaatgttc ttatgtggca attccttgta
     6301 ttcgctacga ggaacagcag ggtctgcgtt aaattgtgac aaaacgatgt tccagatttc
     6361 aatataacgg tcgttttcaa tatcttctgc aagcaggcga agaccgatat tttctgggtc
     6421 aaaggcttcc ccacggtcaa agaagatttc tgtatctggt ccagaaggtc ccgcaccgat
     6481 ttcccagaag ttgtcctcaa ttggaatcaa gtgacttgga tccactccca cttcaatcca
     6541 gcggttgtaa gaatctttat cgtctggata gtaggtcatg taaagttttt cagcagggaa
     6601 atcaaaccat tcagggcttg tcaaaagctc ataagcccaa gtgatagctt cgtcacggaa
     6661 gtaatccccg atagagaagt tccccaacat ttcaaacatg gtatggtgac gcgcagtctt
     6721 ccctacgttt tcgatgtcgt tggtacggat agccttttgg gcattggtaa tacgtggatt
     6781 ttcagggata atggtcccgt caaagtattt cttgagggtt gctaccccag agttgatcca
     6841 caaaagagtt gggtcattta caggaaccaa acttactgat ggttctactg agtgaccttt
     6901 ggtcgcccag aaatcaagcc acatttggcg tacttgtgca ctagatagtt gtttcatatt
     6961 gtctccttat ttacttgttt aatgtgattg gctttccagc atttccacat agtcaatcgc
     7021 gacacagagg gaaatgacta ggcctgcata agcgtcctca agaaccgtta cggtataggt
     7081 agaggtcaga tggaagagtt ccttcttaat ttccgcaatc agctgatcgc gatcatccag
     7141 caatttgaaa ttcaaatccc agatattgcc ctcgatacga agacctagat tatcaaactc
     7201 atacttatct cgccagaagg tcaacttctt acgaatgaca aaactcgagc catcccgaag
     7261 ctgaatctca aaacgaggaa gcaaggtcaa gatttcttta ctgatctgac tgacttgttc
     7321 accagccgca tcatagatgg taaaagtttt ggaaatctta aaaaatgatc cctccacctg
     7381 ataggcaatt tctcccctgt catccttgat agcgaagcgt tcgcctccaa gacgaaactt
     7441 ttgtttgaca agaaatattt tcatcaacac ctccaaaaat caaaagacaa gctcatatca
     7501 cgaagggcga aaaaccgcgg taccaccttc attcaatgaa cttgtcattc tcttgttctt
     7561 atgcaattgt atgattgagt agcatgactt cctagcttag atggctcgca gcaccgccat
     7621 ttctctggac taagacaagt gaaaattaat tctcaacttt cttattataa cgttttttta
     7681 agcttgcgtc aactggaaat gatctccgtt gaattagacc aattccctac atctctgatt
     7741 actttttcag gatatatttt ttcttactgc cgtttttctt tttatcccaa attttcatat
     7801 tactaaacac agctactaga atatttccaa atataaaggt gcctatcacc caatatatgg
     7861 actcagttgt taagtattgt cgatccaagc catcctttaa atggaatagt ataacagttt
     7921 ggttaacaat cataaaggtt ggccagaaac tttttttgaa aaaagtagac attttcatta
     7981 tttgttgccg ctttctgtaa ggttaatact caataaaaat caaaaagcaa actaggaagc
     8041 tagcctcaag ctgtacttga gtacggcaag gcaacgctga catggtttga agagtatagg
     8101 cttagtatac tactaggcaa gcaaataagc aaataagcaa ataaacaact agaatagaaa
     8161 aagatagggc tctaaaaact gacttctatt ccttaaaaac gaaccagctt gactgattcg
     8221 tcttcttacg tttatctcct acttccgata cattttaaac tgtaggaaga ggtcgctata
     8281 tttccctgtc catttatggt caaatttctc ataaacttct aggtgtttca tggtttcaac
     8341 atcgggatag aaggccttat cttcctttgt ttcctctggg agcaattcct tcgctggtag
     8401 gtttggtgtt gaatagccga catactccgc attttggaga gcattttcag gtttcaacat
     8461 aaagttgata aaggcatagg ctgagttttg gtttttaact gttttgggaa tgaccatatt
     8521 gtcaaaccaa agattgctgg cctctgtcgg taccacataa cgtagatttt catttttttc
     8581 taacatttgg ctggcttcac cagagaaggt cacgccgatt gcaacattat tctgaatcat
     8641 atagcccttc atctcgtccg caacgatagc cttgatattt ggagtcagtt tgtagagctt
     8701 atccactgtc tcttccaact gctgcagatc cttggagttg aggctgtagc cgagggaatt
     8761 gagtcctagt cccagcacct cacgcgcccc atcaaagagc atgatagaat tcttatactc
     8821 cggcttccaa aggtcatccc aatgctcagg cgcttcatct accatggttt cgttgtagac
     8881 aattcctaag gttccccaga agtaagggat ggagaattta ttacctgggt caaaggactg
     8941 gttgagaaac tctggtccga tattttccat tccttcaatt tttgaataat caagcggaac
     9001 caagaggtct tcgtccttca tcttgttaat catgtattca cttggaatgg caatatcgta
     9061 ggtcgttcca ccctgcttta tcttagtgta catggcttcg ttggagtcaa aagtctcgta
     9121 ctgaacttga attcctgttt cttctgtaaa ctgagtcaag agttcaggat cgatatagtc
     9181 tccccagtta tagataacca atttttgact atctcgacta ttgattttac tatctaaatg
     9241 agtcgcaatt ccccacaaga caaggataat cgctgcaatt cctgctaaaa atgaatagat
     9301 ttttttcatg cttgctcctc cttctcacga gagataaagt aataacctac aactaggata
     9361 atactaaaga gaaagactag agcagaaagg gcattgattt ctagcgaaat ccccttgcga
     9421 gcacgagagt aaatctcgac tgatagggtt gaaaagccat ttcctgttac aaagaaggtc
     9481 acggcaaagt catctaacga ataggtgaag gccatgaaat aaccagcaat gatagacgga
     9541 gtcaggtaag gaagcatgat ttccttaaac atctgaaatt gactagctcc caagtcatag
     9601 gccgcatgaa tcatgtcgcc attcatttcc ttgagtcgag gcaagaccat caagaccacg
     9661 ataggaatgg agaaggccac gtgactagat agaacggtca aaaagccaag tgaaaacttg
     9721 agttgggtaa agagaatcaa gaagctagca ccaatcataa cgtcaggcgc aaccatgagg
     9781 atattattga gtgatagaaa ggcttcttgg tatttcttac gagactggta gatgtaaatg
     9841 gcaccaaaag tcccgataat ggtcgctatc aaggctgata ggaaggccaa gaaaaatgtc
     9901 tgagccaaaa tcagcatgag tctcccatct ccaaacatgg tttcaaagtg agtccagcta
     9961 aaacctgtaa agctattcat atcatcacca gcattaaagg catagccaat caagtaaaag
    10021 ataggcaggt agaggaccag aaagaccagt cccagataaa ggttggcaaa ttttttcatc
    10081 gttctctcct ttccttagtc acccacatgg tgatgaacat ggtcaggatg agaatcacac
    10141 cgatagttga acccatacca tagttgtcat tggttagaaa attctgctca atagccgtcc
    10201 ccaaggtgat aacgcggttc ccaccaatca aacgggtcag catgaagaga ctcaaacttg
    10261 ggataaagac cgactgaacc ccacttcgca caccgttcat agatagaggg aagatgacat
    10321 gacggaaggt ctcccactta gttgcaccaa ggtcgtaact agcattgatg agattattat
    10381 ccatatcgtc taagacattg aaaatcggca aaatcataaa ggggagctcg atgtagcttg
    10441 cgacaaagat aaatgagaaa tcagtaaata gcaactgctg tgaaccaatt ccgataaatt
    10501 ccaagaattg gttaatagag ccattctgac caaaaatccc gataaaggca taagccttaa
    10561 gaagaaggtt gatccaagtt ggcagaataa tcagcataat ccagagttga cggtgtttga
    10621 gacgggtcaa aaagagggcc gtcggataac tgataagcag tgtcacaaag gtcacaatgc
    10681 ctgcataaag cactgagttg aaactcattt taagataggt caagttttgt gacgcaaagt
    10741 aagatttgta attttctaaa ctgaactggc cttcgatgtt gaaaaaggat tgaccgaaaa
    10801 tcaagaccaa gggtgccaat acaaagagcg caatccaaag catgtagggt actacaaaga
    10861 gtttagagct tgttttcttc atctctttcc tcctcgattg cattgatcaa acctgcttct
    10921 tgctcttcga tttctacgta ctcctcaata cgagcatcga actcttcttc ggtttcattg
    10981 agacgcatga tgtggatgtc ttctggttca aagtccagac cgatttcctc acccacgatg
    11041 gccttacggg ttgagtggat catccattca tttccaagtt cgtcataggc gataatttca
    11101 taatgcaccc cacggaagag ctgggtatcg accttaactt gaagcttgcc ttcttcagga
    11161 agggtaatgc gcaagtcctc tggacgaata acgacctcaa caggttcatt tggcttcatc
    11221 ccaccatcaa ccgcttcaaa gcgtttgccg ttaaattcga ccaagtagtc ctcaatcatg
    11281 gtacctggca agatgtttga ctccccgata aaggtggcaa caaagtggtt gattggctca
    11341 tcgtagatgt ccacaggggt tccagactgg acaatctcgc catcattcat aacgaaaatc
    11401 cagtcactca tggcaagagc ttcttcctga tcgtgagtga caaagacaaa ggtaatgccc
    11461 aatcgttgtt gcagttcacg cagttcgtac tgcatgtctg ttcttaattt caagtccagc
    11521 gctgacaagg gctcatccaa caaaaccaca cggggttggt tgatgatagc acgggcgata
    11581 gccacacgct gacgttgtcc tccagaaagt ttgcggatgg aacgtttttc ataaccttcc
    11641 aactgaacca tcttgagaac ttccgctaca cgttgctcga tttctttctt atcaatttta
    11701 cgcaagcgaa gtggaaaggc aacattttca aacacattca tatgtgggaa caaggcatag
    11761 gattggaaga cagtatgtac gtcgcgcttg ttggttggaa tatcattgat acgaacatcg
    11821 tctagcatga tatctcctgt cgtcgcatcc agtaaacctg caataatgtt taggatagtt
    11881 gatttccccg aaccagatgc acctagaagg gtgtagaatt tcccttcttc caactcaaag
    11941 ttgatgtctt tgagaacctt ggtgttgctg tcttcaaaaa ctttagagac gtttttgaat
    12001 tcgataattg gttttttcaa ttggcataaa ttccttcttt ttcatagatt aaccgatcgg
    12061 ggctctgtca ggtccccact acctcttgca gggagtaaaa ccacctgcat acatcttcgc
    12121 taccgatagg ctttcacccc cctttccatg acatagcagc agcttttcaa atacagcttc
    12181 ctacttgctt tcacccaaga tccggacttc tctttcaagc gtaatacctg agtgttcctt
    12241 gactttttcg ataaccgatt ggatcaagtc ctcgtagtct ttggccgttc catctgcgac
    12301 attgatcata aatcctgcat gcttttctga cacttctacg ccaccgatac gatagccttt
    12361 caagccagct tctgaaatta actgacctgc aaaatgcccg actggacgct taaagaccga
    12421 gccacaagat gggtattcca aaggttgctt gagttcacgt aggtgcgtca agcggtccat
    12481 ttcctgcttg ataacctgat gggttcctgg ggctagggca aatttaactg acaagacaac
    12541 tgcaccagac tcctgaatag ctgaatggcg gtaaccaaaa gccaagtctt tagcagacag
    12601 ggtttcgatt tctccatcct tggtcaagac cttacaagac tgcaatatgt gagcaatctc
    12661 gccaccatag gcacccgcat tcataaagac agcaccgcca acgcttcctg gaataccaca
    12721 agcaaactca aagccagtca aactatgacg gagggcaatg cgagttgttt caatcaagtt
    12781 agccccagct tctgcttcaa tggtatagcc atcaacagaa acgttattga gcttgtcaca
    12841 caagatgaca aatccacgaa tcccaccatc acgaacgatg atattgcttg cattgccaag
    12901 aaccatccaa gggatatttt cttggttggc aaatttcaca aggcgagcca actcaaaacg
    12961 atttcgtgga aagaccaaat aatcagcctc tccacctact tttgtataac tatagctatg
    13021 caagggttcc ttaaaacgga tatcaattcc ttctaagatt tcaagcattt tttctcttac
    13081 agacatgtca ctcttccttt tacaaaattc attccattat accattttta gagacatttg
    13141 acgaccataa aaataccttg tttggatttt gcataagaaa aagaggttcc ccccttttta
    13201 tgatttttta caaaagattt ccttggttcc ataggcgacc agaacgagct ccagtgctag
    13261 aatcacttca accaagactg gatttgtcaa ccagcctact tgggaaagag atggtgccag
    13321 atcaaagaag gcatgcaagc cataggctgc taggagataa atccatttct tctggcgaac
    13381 agcttggtaa acccaaactg tcaaaagtaa ttggaaacca agcgccaaga ttcgctcaaa
    13441 accaagcaaa taaatctgcc agaccgaaag tgactgaatg gtttttaaca tattttcaga
    13501 cagtaattgc ataacctgtg gattctgagt ttgaactgcc gaaagaacaa tgtaaagatt
    13561 gagtaaacta gtaaggccta gaaaaatcaa ctccaagcca ccatgcccta ggccataggc
    13621 caaggcatct gccttttcca aacttctctt tttctccaac catttgaaga aaacaagacg
    13681 agccgtttcc tcaaaaaatg ctgccatggc taggccatag ataatataga taagcggatg
    13741 atcttacaag agggcaatac taccgtcttt ttgaggatgc aaaatcaaga tatgcaccag
    13801 tttttccaaa atctgcgaag agacaaagaa agcgactgca cccaagccca agacagctag
    13861 attaatctgg tatttctttt tggcaaacca aatgcttcct atcagaaaag ctagcaacag
    13921 caacatggta ataatgatat gaatggtcat tttcttctcc tattctgcct tttcaatatc
    13981 ttttttcatc tcgtcaacat tgaacttagc aaacaagtat tgacggtctt ggactgggaa
    14041 acgttggtcc aactggtcaa ctgctcccac ctcgataatg ccttccttga tgacaaaatc
    14101 catgacatgt ccaccgaagg tcaaatcatc tgagatgaag tgcagatggt agcctgccac
    14161 actgacccca tggaaaatct caggcgtcca gaaaccaaca atagtcccca caacattgtc
    14221 acgactatat tccggttgat gggttgcgac atcagcaaac ttggtatcgg gtgttgactt
    14281 aggaatcata cgcacatgca tatgtgaaaa ttctccccga atcttgatag agcggaaaag
    14341 attttcccca tcataataag actcaattcg ttcttccaat tccttgtctg tcatctcaaa
    14401 gcgctggcgg aaaatgacct ctgcctgatg cggtactact gctgcataag gaataagggc
    14461 atctggtgac acttccacaa tttctggctg ttctcctgtt ccttttgcct gataagcctt
    14521 accatctaag acaatcaatt ccccatcaat agaatccaag gttcccaaac caaggtcacc
    14581 atgttctagc aattctccta ctgtcatagt accaccataa aggccagcca tcaaggctcc
    14641 aagagtattg tattgaaata atttcactgg ttcctgcacg ttcttatcca ttcactttct
    14701 tgtctgttat tctataaatc ttactcctaa gtataccaca tttgccccta gatgtgaacg
    14761 agagaaacgc tctagacatt gccaagaagg aaaaaaaagg gtacaatgta acaaaatcaa
    14821 gggaggtctg gaatgaagaa acaaagcaag tacaaagagg tcgtttccta tctgaaaaat
    14881 ggtatcgagt ctgaacgatt tccgacgggt agtcgcctgc cttctatccg tcaactgagc
    14941 cttgactttc actgcagcaa ggacaccatt caacgagccc tgctggaatt acggcacgaa
    15001 caatacctct atgccaagcc ccagagcggc tactatgtat tagaacaagg gcaacatcaa
    15061 gacctagaaa tcgaggttac cgacgaacat gccagtgcct atgacgattt ccgactctgt
    15121 gtcaatgaaa ccttgattgg acgagaaaac tacctcttca actactatga caatcaagaa
    15181 ggattagaag acctaagaca gtccattcac aaactcctat ttgagcaagc tctctactgc
    15241 aaggctaacc aactagtact gacttctgga acccaacaag ccttatttat cctctctcaa
    15301 atatcctttc ctagacaagc caaggaaatc ttggtggaac agccaaccta ccatcggatg
    15361 aatcgcctct tgattgcaca ggggctggac tatcaaacga ttgaacgagg cattgatggg
    15421 attgacttgg aggagctgga aggccacttc aaaacaggaa aaattaaatt tttctacacc
    15481 attcctagat ttcactatcc tctgggacat tcctattctg agcaagacaa acgatctatt
    15541 cttaacttag ctgccaagta tgatgtctat atcgtagagg acgattatct gggtgatttg
    15601 gactccaaga agggccaaac cttccactat cttgatacag aggagcatgt catttatatc
    15661 aagtccttct cgaccagcct ttttcctgcc cttcgtatta cagcactcat tcttccaaat
    15721 actatcaaag aagcatttgt ggcctacaaa aatatcctag actacgacag caacctcatt
    15781 atgcaaaagg ccctgtcact ctatattgac agtcaattgt ttgaaaaaaa tcgtttggct
    15841 cgcttgacca atcatgaatc ttaccaaaaa caaatcgagg aaaggataac taaaacacct
    15901 tgtccccttc ctcattatcc cctacacgat ggtttattgc tagacctgag acagtatcct
    15961 aaaatcgcca gtctcaaaca cagtcaactg ggcttggact tctttgaaga ggcctattta
    16021 agcacctgtc cttatcaatt tgccaaggtg tccctagaca atctggaaaa tgttttaaac
    16081 tatttaaaag cagaattgga atgacatcca actctgcttt ttcttattct tcaacttcag
    16141 ttaaacttgc tgctttttca agatagcttt ggtaagttcc ttcatcctta agtttttgga
    16201 tgaccttgtc taccacttct ttcaaatcat cactattttt tctaagagca acggcattgg
    16261 cgtcgccgtc cttcatcttc aagctgacag ttgcaacagc taagccagcg tttttagcag
    16321 cataactaag tgcaacaggc tcatccatat gaacagcatc tacttttcca gcctgcaatt
    16381 cattgactgc ttcacccata ttagttaggg aagttaattg agcttttggc aattgttcct
    16441 tgaccattga ttctggaaca gtcccttttt gggctgcaat attagcactt tctaggctag
    16501 ttaaatcctt gtatttttcc acatcagcct tacgaaccaa gaaactaatc ttgttttcat
    16561 agtatgggat tgaaaaatca aagacttctt ttctctcgtc agtagcacta attcctgcaa
    16621 ctgctaggtc agccttacca gtttgaagac tggtcaaaac attgtcaaaa ctcatgcttg
    16681 agatttccaa cttaacccca agttcatcag cgatagcctg agccatgtcg atgtctgcac
    16741 cgactacctg gttctttcca tcaaccaatg attgaaattc aaagggtgca tagtcaggac
    16801 tggttgccac aactaatttc cctttttgtt tgatagcatc cacagcagac tgagaaccat
    16861 tagaacctga ctggcaagct actaagaaga aacttgcaag aaaattacat aaaacaaata
    16921 tccatttttt tgatttcata aataaacaac ctctctgtta attttgtata attataacga
    16981 tatccaagtt acttgtcaag tgttttttaa att
//
LOCUS       DAVGJJ010000037        16798 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000037 DAVGJJ010000000
VERSION     DAVGJJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.037"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            33..1703
                     /locus_tag="VVE35_001140"
     CDS             33..1703
                     /locus_tag="VVE35_001140"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU3061340.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2031..3206
                     /gene="mutY"
                     /locus_tag="VVE35_001141"
     CDS             2031..3206
                     /gene="mutY"
                     /locus_tag="VVE35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU3061341.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            3261..3965
                     /locus_tag="VVE35_001142"
     CDS             3261..3965
                     /locus_tag="VVE35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061342.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            3958..5307
                     /gene="vicK"
                     /locus_tag="VVE35_001143"
     CDS             3958..5307
                     /gene="vicK"
                     /locus_tag="VVE35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU3061343.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            5309..6118
                     /locus_tag="VVE35_001144"
     CDS             5309..6118
                     /locus_tag="VVE35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU3061344.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            6209..6451
                     /locus_tag="VVE35_001145"
     CDS             6209..6451
                     /locus_tag="VVE35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU3061345.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            6453..6707
                     /locus_tag="VVE35_001146"
     CDS             6453..6707
                     /locus_tag="VVE35_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU3061346.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            6782..8653
                     /locus_tag="VVE35_001147"
     CDS             6782..8653
                     /locus_tag="VVE35_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU3061347.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVT
                     QHNSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            8818..12072
                     /locus_tag="VVE35_001148"
     CDS             8818..12072
                     /locus_tag="VVE35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU3061348.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(12370..13356)
                     /locus_tag="VVE35_001149"
     CDS             complement(12370..13356)
                     /locus_tag="VVE35_001149"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU3061349.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVSKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            13550..16018
                     /gene="gyrA"
                     /locus_tag="VVE35_001150"
     CDS             13550..16018
                     /gene="gyrA"
                     /locus_tag="VVE35_001150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU3061350.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            16018..16761
                     /gene="srtA"
                     /locus_tag="VVE35_001151"
     CDS             16018..16761
                     /gene="srtA"
                     /locus_tag="VVE35_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU3061351.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         5305 a         2902 c         3688 g         4903 t
ORIGIN      
        1 aattatcata taagaggtta gaggaatttt gaatgaaaac agatattgaa atcgcacaga
       61 gtattgagtt gaagccaatt gttgatgttg tagagaaact tggtatttct tacgacgatt
      121 tggagttgta cggaaagtac aaggctaaac tcagctttga taaaattcgt gcagttgaga
      181 gcaatccagt cggtaaattg atcttggtta ctgccatcaa cccaacacct gcaggtgaag
      241 gaaagtcgac gcttaccatt ggtcttgcgg atgccttgaa caagattggc aaaaaaacca
      301 tgattgctat ccgcgaaccg tctcttggtc cagtcatggg gatcaagggt ggtgctgctg
      361 gtggtgggta tgcacaagtt ctgccaatgg aagacatcaa cctccacttt actggagata
      421 tgcatgctat tacaactgcc aacaatgccc tttctgcctt gattgacaac cacttgcacc
      481 aagggaatga gctgggaatt gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga
      541 acgaccgtgc gctccgccat gtgactgttg gtcttggtgg tcctctaaac ggtattccac
      601 gtgaggacgg ttttgatatt acagttgctt cagaaatcat ggcaattctt tgcttggcaa
      661 cggacatcga ggacttgaaa cgtcgtttgg cgaatatcgt tattggttat cgctatgacc
      721 gtacgcctgt ttctgtaggt gatttgcagg ttgagggtgc cttggctttg attttgaagg
      781 atgctattaa gccaaacttg gttcagacaa tttacggtac acctgccttt gtacacggtg
      841 gtccatttgc caatatcgct catggctgta actctgtttt ggcgacgaca acagcccttc
      901 acttggctga ttacactgtt actgaagctg gttttggtgc ggaccttggt gctgagaaat
      961 tccttgatat caagacacca aacttgccaa catctccaga tgcagttgtt attgtcgcaa
     1021 cccttcgtgc ccttaagatg aatggtggtg tggctaaaga cgctctgact gaagaaaatg
     1081 tagaggcagt tcgtgcaggt tttgctaact tgaaacgcca cgttgaaaat atccgtaagt
     1141 tcggtattcc agcagttgta gctattaacg aatttgtatc tgatacagaa gctgaaattg
     1201 cagtcttaaa agaactctgt gcctcaatcg atgtaccagt tgaattggct agtgtctggg
     1261 ctgatggagc agaaggtgga gtagcacttg ccgaaacagt tgttaagaca attgctgaaa
     1321 atccagctaa ctataaacgt ttgtatgata atgacctttc tgtccaagaa aagattgaaa
     1381 aaattgtcac tgaaatctat cgtggtagca aagtgaactt tgagaagaaa tctcaaacac
     1441 aaattgctca aatcgttcaa aacggttggg acaaattgcc aatctgtatg gctaaaactc
     1501 aatacagttt ctcagacaat ccaaatgcgc ttggagcacc agaaaacttt gaaattacca
     1561 ttcgtgaatt ggtaccaaaa ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta
     1621 tgaccatgcc aggtcttcca aaacgtccag cagctctcaa catggatgtt gaaagcgatg
     1681 gaactgtact aggcttgttc tagtggattg aaattgactt taagtgagtt tggaaataat
     1741 atccaagctc attttttctc agactgcaag cagttcatca ataagattta tactcttcga
     1801 aaatctcttc aaactacgtc agcttcacct tgccgtaggt atatgatact gactctgtca
     1861 gttctatcca caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc
     1921 tctttgattt tcattgagta taagtagata gaaaagttat atactatcta ttgaaaatat
     1981 tataaatcag atacaaagac aggtgaaacc ttctgtgtta tactagagat atgttagatt
     2041 tgaaagaata cggtatcgtc atgtggccgg aggagaaagt catttctttc cgtgagaaac
     2101 ttctcgcttg gtatgatgaa aacaaaagag atttgccttg gaggagaagt aaaaatcctt
     2161 atcacatctg ggtatctgaa atcatgcttc agcagaccag ggtggataca gttatccctt
     2221 actacgaaag attcctggac tggtttccaa ctgtcgaaag tctggcaact gcgtctgagg
     2281 agagtttact gaaagcttgg gagggcttgg gctattattc tcgagttcgc aatatgcagg
     2341 ctgcagccca gcagattatg actgactttg gtggccaatt tccaaatacc tatgaaggaa
     2401 tttccagctt gaaagggatt ggaccttaca cagcaggagc catttccagt attgctttta
     2461 acttgcctga gccagctgta gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag
     2521 tcaaccacga tattgggatt ccaagtaatc gaaaaatttt tcaggcaatg atggagatct
     2581 tgattaatcc ggatcgtccg ggtgatttta atcaagcatt gatggactta gggtctgata
     2641 ttgagtctcc tgtaaatccc agacctgaag aaagcccagt caaggacttt agtgcggcat
     2701 atcagaatgg aaccatggac cgttatccaa ttaaatctcc taagaaaaag ccggttccta
     2761 tttatcttaa agccttagtt gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg
     2821 aaagcgaaaa gctcttggca ggtttttggc atttcccctt tatagaagtt gataactttt
     2881 cgcaagaaga gcagtttgac ctctttcatc aggttgcgga agaaagtgtg aactttggtc
     2941 ccagtccaga agagagtttc cagcaggact atgacctaga tgttgattgg cttgatgttt
     3001 gttttgatac agtccagcat gtttttagtc atcgaaaatg gcatgtacag attgtagcag
     3061 gtcaggtgag tgacttccat gatttttcag atagggaagt tcgttggctt tcaccagaag
     3121 aattcaagaa ttacccactt gccaaacccc aacaaaaaat ctggcaggct tatgcacaag
     3181 ccaacttaga cagtagccaa gactagctat tgtgtcttct gactattttt tggtataata
     3241 gtagagaaaa aggtgaacat atgaaaaaaa tactaattgt agatgatgag aaaccaatct
     3301 cggatattat caagtttaat atgaccaagg aaggttacga agttgtaact gcttttaatg
     3361 gtcgtgaagc gctagagcaa tttgaagcag agcaaccaga tattattatt ctggatttga
     3421 tgcttccaga aattgatggt ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc
     3481 ccattcttat gctttcagcc aaagatagtg aatttgataa ggttatcggt ttggaacttg
     3541 gggcagatga ctatgtaacg aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag
     3601 ctcttctgcg tcgttctcaa cctatgccag tagatggtca ggaagcagat agtaaacctc
     3661 aacctatcca aattggggat ttagaaattg ttccagacgc ctacgtggct aaaaaatatg
     3721 gcgaagaact agacttaacc catcgtgaat ttgagctttt gtatcattta gcatcgcata
     3781 caggtcaagt catcacgcgc gaacacttgc ttgagactgt ctggggttat gactattttg
     3841 gtgatgtccg tacagttgat gtgactgtac gacgtctgcg tgagaagatt gaagatacgc
     3901 ccagccgacc agagtatatc ttgacgcgcc gtggtgtagg gtattacatg agaaataatg
     3961 cttgatttac tgaaacaaac catttttacc agagatttta tctttatcct gattttgtta
     4021 ggtttcatcc ttgttgtgac cctcttatta ctggaaaata gacgtgataa tattcagttg
     4081 aagcaaatca atcaaaaggt taaagatttg attgcaggag attattccaa ggttcttgat
     4141 atgcaaggtg ggtctgaaat caccaatatt accaataatt tgaatgactt gtcggaggtt
     4201 attcgtctca ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag tattctgttt
     4261 tatatgacag atggggttct tgcgaccaac cgtcggggtc agattatcat gattaacgat
     4321 acagccaaga agcaactggg gttggttaag gaagatgttc tgaatagaag cattttggaa
     4381 ttgctcaaga tagaagaaaa ctatgaattg cgtgatttga ttacccaaag tccagaattg
     4441 ttgctagatt cccaagatat caatggtgaa tatttgaacc ttcgagttcg ctttgccttg
     4501 atacgtcgag agtctggctt tatttcaggt ttggtggctg ttttgcatga tacgacggag
     4561 caggagaagg aagaacgcga acgaagactc tttgtttcca atgttagcca tgagttacgg
     4621 actcctctga ctagcgtaaa atcctatctt gaagccttgg atgagggggc tttgtgtgaa
     4681 actgtagcac cagactttat caaggtttct cttgatgaga ccaaccgtat gatgcgcatg
     4741 gtgacggatc tcctccatct ttcacgtatt gataatgcta ccagtcacct agatgtggaa
     4801 ctgattaact tcactgcttt tattaccttt atcctcaatc gttttgacaa gatgaaagga
     4861 caggaaaagg agaaaaaata tgagttggtg agagattatc ccatcaattc tatctggatg
     4921 gaaattgata cagataagat gacgcaggtt gtcgacaata ttttaaataa tgctattaag
     4981 tattcgccag atgggggtaa aatcactgtc agaatgaaga caactgaaga ccagatgatt
     5041 ttatccattt ctgaccacgg tttggggatt cctaagcagg atttaccacg tatctttgac
     5101 cgtttctatc gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg tctaggactg
     5161 tctatcgcta aagaaattat taaacaacat aagggcttta tttgggccaa gagtgaatac
     5221 ggcaagggtt caacctttac cattgtgctc ccttatgata aggatgcagt gaaagaagaa
     5281 gtatgggagg atgaagtaga agactagaat gagtgaaata ggctttaaat acagtatttt
     5341 agcgtcgggt tccagtggaa attcttttta tctggaaacc tcaaaaaaga agcttttagt
     5401 ggatgcaggc ttgtctggca agaaaattac cagtctgcta gctgaaatta accgcaagcc
     5461 agaagacctg gatgccatct tgattaccca tgagcattca gatcatatcc atggagtagg
     5521 cgttttggct cgcaagtatg gtatggatct ttatgccaat gaaaagacct ggcaagctat
     5581 ggaaaatagt aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg
     5641 taaaaccaaa acttttggag atatcgacat cgagagtttt ggtgtaagcc atgatgcagt
     5701 cgcaccgcag ttctatcgct ttatgaagga tgacaagagt tttgtccttt tgacagatac
     5761 aggttatgtc agtgaccgta tggcgggcat tgtcgaaaat gcggatggct atcttatcga
     5821 ggccaaccat gatgtagaga ttttgcgatc aggttcttac gcttggcgac tcaaacaacg
     5881 aatcctatct gaccttggtc acctttctaa cgaggacggt gctgaagcta tgattcggac
     5941 gctaggaaat cgtactaaga agatttacct tgggcattta tctaaggaaa acaatatcaa
     6001 ggaactggct catatgacca tggtcaatca gctggctcaa gctgatctgg gagtcggagt
     6061 agactttaag gtttatgata cctcaccaga taccgcaaca ccattgacag agatatagaa
     6121 agaacgctga gaaggtgttc tttttatatt gactgaacac ctaaaaagta atacaatggt
     6181 gttaccatta aaaaagggag cacaaaagat gactactatt acattaaaag tttctgaagc
     6241 tgataaaaca tttatgaaag caatggctaa gtttgaagga gtttcccttt cggaacttat
     6301 tcgcaccaaa actcttgaag ccctagaaga tgaatacgat gctcgtgtgg cagatttagc
     6361 ctatcaagag tatttagaag acttggaaaa aggagttgaa cccattactt gggaagaaat
     6421 gatgcatgat ttaggcttga aggatgaata atttgtataa attagttcca acaagacgtt
     6481 ttatcaagca attgaaaaaa ttggaccgtt atacgcagaa gctaattaca aactatttac
     6541 aaaccaatgt tttggaagac ccaagacgac acggaaaggc tttggttggt aatcgcgttg
     6601 gtcaatggcg ctatagaatt ggtaattatc gagttatcgt acaaattgta gatgatgaat
     6661 tagtcgttgc tactctagaa gttggtcatc ggagagatat ttattgaatt acttttttca
     6721 agtaaatttt cattctttaa aatatgatat aatagggctt acaatataag agaggttgct
     6781 gatgacaata gatattagtg aagaaagttt ggctaaagaa tcggctgact tattaaaaat
     6841 tcttttaaaa gatcgaacga caaagaaatc aattgtttgg gctacgcatt cttatgaatt
     6901 gttaggaaag ggatttgctc caagtgatcg catcaatcca agtaaggtta ctggaaactt
     6961 tgccaacttg attcagccca gatctgaaaa atccaaatat gaacaaaaag atcggacaaa
     7021 aataagggca gaagtgttta caccaacgtg gttggtcgca aagcaaaatg gatatgtaga
     7081 atcgaaatta ggctcgttaa gtcttgagga gtatgtggac ttacgatggt tagaggtaac
     7141 ttgtggtgaa gcaccttata tggtaacgag atatgatacc gtaactggtg aggaaattcc
     7201 attatctgag cgagttggtt ttgttgatag aaaactgcaa cggattagtc gtgaggtttc
     7261 agatgaagtc acattttata aacttgtaaa agatgcttat catgcttcgt atggttatga
     7321 gtatcaagga gattctctcc ttttggcgcg tgagaatctt ttggcaactt ttgaggacta
     7381 ttatctagct aagactggaa atcaaccaac attagagcaa aagaaagaaa ttgcgactat
     7441 tatctcttac aatgtctttc agatggatgg attgaaaaaa agtagtcctt attcagctaa
     7501 acaaaagcaa tctcaacaat tgagtttgtt tgcggatgag ctagaagtcc aagaagtaga
     7561 agaatctaaa acccatatca aagactggaa aaagaataga atgataggtt ttgagcgcct
     7621 atctagcgag gaaagtgaaa tgaaatttga tgtcgtgata ggaaatcctc cgtatcagga
     7681 aacaggagaa gccagagatg agccgatata tcactatttt attgatgaag cttataaaat
     7741 cgcagataaa tctatactaa tcaccccagc aaggttttta tttaaagctg gtcaaactcc
     7801 taaaaattgg atggaaaaga tgcttgaaga caagcattta aaagttcaat tctatgaact
     7861 taaaagcggt aaagtattta caggaacgga tattaagggt ggagtagcaa taacttatcg
     7921 tgatgttgat aaagatttgg gaccaatcgg agtatttact atttttgaaa ctttgaatgg
     7981 tattgtaaaa aaaatcgaaa aacatccaga atttgaaact ttttctgatt tagtttatcc
     8041 tcaaggtatt tatagatttt caaataaact tttttctgat tatccaaatg ctgaaaagct
     8101 gcaaggaaaa gggacaaaaa ataaaattgt ttcgaaatca ctaactcagt tagattttgt
     8161 tttcaaaaac aacgaagaaa aagaccatgt tgctctactt ggaagaattg gctccgaacg
     8221 tgtttatcga tatattaata aaaaatattt agatttaccg gaaacattcg aaaattataa
     8281 tgtttttgta ccagaagcta atggaagtgg tgccttaggt gaagtcttat caacacccct
     8341 aatcggggaa cccctaatcg ggcatacaga tactttttta tctattggta attttaaaac
     8401 aaaatttgaa gccgatgctt gtattaaatt tattaaaact aaattcgcta gagtattatt
     8461 aggtgttttg aaagttactc agcataattc acgcaaaact tggtattacg tccccctcca
     8521 agactttacg gtcaattcgg acattgattg gacacaatca gtgactgata ttgaccgcca
     8581 gcttgatcaa aaatatgacc tttcccctga agaaattgcc tttattgaga atcatgtaag
     8641 ggagatggat tagaaaagta tttttatttg acaaatagtg ctcaatgatc taaaatgact
     8701 atataggatt aggtcaggaa gcatacgatg ccctgaccct ttttgtactt atgagatgag
     8761 aaagtcattt gttagataaa ttgacttcgt tagcaaacgt tcaaaaaagg aaaacttatg
     8821 ccagtagaaa ttaaaaccac taaagaaatt catcctaaaa tctatgccta caccacaccg
     8881 acagtaacca gtaatgaagg ctggattaag attgggtata cagaacgtga tgtcacacaa
     8941 cgtatcaagg agcaaacgca tacagctcat atagctacag atgtcttatg gactggtgat
     9001 gcagcttata cagaagagcc tgataagggg aaaactttca aggaccatga tttccaccat
     9061 ttcctttctt tccatgatgt agaacgtcgt cccaagacgg aatggttcta ttttaatgga
     9121 actcctgaaa aatcaaaaaa tctttttgat aagtttgttc agcatgattt gtctggttat
     9181 cagcctggaa aaggacagga ctatactctg cgacaagagc aagaagaagc agttgctaag
     9241 acattagctt atttccaaga acatgctgga ggcaagtttc tctggaatgc caagccacgc
     9301 tttggtaaaa ccttgtctac ctatgaccta gctcgacgga tggaagctgt caatgtccta
     9361 attgtaacaa accgccctgc cattgctaac tcatggtatg atgattttga aacattcata
     9421 gcaggtcaaa cgacttacaa gtttgtttct gaatcagata gccttaagag tcgtccaatc
     9481 ttgtcacgac aagaatttct tggtatttta gctgacgatg taagacaact tgcttttatc
     9541 agtctccaag acttgaaagg atctgtttat ttaggtggag agcacgataa actcaaatgg
     9601 gtaactgatc tgcattggga cttgttggtt attgacgagg ctcatgaagg agttgatacc
     9661 ttcaagactg accaagcctt taataagatt cgacgaaatt ttactctgca tttgtcaggt
     9721 acatcattta aagcattggc taaaggagat tttacagagg aacaaatcta caactggtct
     9781 tatgctgatg agcaggctgc taagtattcg tggtctcttg agcaagaaga ggaaaatcct
     9841 tatgaaagct tgcctcagtt gaatctcttt acctatcaaa tgtctcagat gattggcgaa
     9901 aagttagaaa aaggcgctca gatcgatggt gaaaatattg actatgtttt tgacttaagt
     9961 gaatttttcg ctacagatga taaagggaaa tttattcatg agcatgatgt cagaaattgg
    10021 ttagatactc tatcaagcaa tgaaaaatat ccattttcaa ccaaagaact ccgtaatgaa
    10081 ctcaagcata ctttttggct tttagaacgt gtcgcttcgg ccaaagcatt aaaagcccta
    10141 ctagaagaac acccaatcta tgaaaactat gagatcgttc tagctgctgg tgacggacgt
    10201 atgtccgaag aagacgataa agtcaaactc aaatccttgg acttggttag aaaagcgata
    10261 gcagagaatg acaaaaccat taccctatcc gttggtcagc tgacgacagg tgtcactatc
    10321 cctgaatgga caggtgtatt gatgttatca aatttgaaat caccagctct ttatatgcag
    10381 gccgccttcc gtgctcaaaa tccttactca tggagcgata acaaaggaaa tcactttcgc
    10441 aaagaaagag cctatgtatt tgactttgcg ccggaaagaa ccttgattct ctttgatgag
    10501 tttgccaaca acttatcgct tgtaactgca gctggtagag gaacttcagc tacacgcgaa
    10561 gaaaatatta gagaattatt aaacttcttt ccaattattg ccgaagaccg tgctggtaag
    10621 atggttgaaa ttgatgcaaa ggcagttcta accactcctc gccagataaa agctagagaa
    10681 gttcttaaac gaggttttat gtccaatctc ttatttgata atattagtgg tattttccaa
    10741 gcaagtcaaa cagttttaga tattttaaat gagctgccag ttgaaaagga agggaaggta
    10801 caagatagtt ctgatttatt agatttttca gatgttacag tcgatgatga gggaaatgca
    10861 gtagtagacc atgaaattgt agttaatcag caaatgcgac tttttggtga aaaagtttat
    10921 ggacttggtg aatctgttgc tgagttagtc acaaaagatg aggaacgaac tcaaaaacag
    10981 ctggtcaatg acttgagtaa gaccgtttct tcagtgattg tagaggaatt gaaagcagat
    11041 tattctctaa aaacaaggga aactgagcaa attaagaaac aaattacagc aacacttgag
    11101 aatgaaattc gaaaaaatga tatcgaaaga aaaatttctg aagctcatat caagcaagag
    11161 ttgcaacagc agctcaaaga agcaaatgat aaagcgcaaa aagataagat tcaagaagat
    11221 ttggaaaaac gtttagaaga aaataaactc attcataaag aaaaactaga acaaacactc
    11281 aaaaaagaag tggaaaaaat gcctgagaaa tttatcgaac aggttgagat aaaacgtgtg
    11341 gaacagttga aacaatcagc tcaagatgaa attcgtgacc atttacgagg gtttgcaaga
    11401 acaattccaa gttttattat ggcttacggt gatcaaactc taacacttga taattttgat
    11461 gcctttgttc ctgaacatgt tttttatgaa gtaacaggga ttacgattga tcagtttaga
    11521 tatttgcgag atggtgggca ggattttgca gggcatctct ttgataaagc aacatttgac
    11581 gaagctattc aagaatttct tcgcaagaaa aaggagttgg cggattattt taaagatcaa
    11641 aaagaagaca tttttgacta tattccaccg cagaagacca accaaatttt cactcctaaa
    11701 cgagtggtga aaaggatggt agatgatttg gaaaaggaaa atccagggat ttttgatgat
    11761 ccatctaaga cttttattga tttatatatg aagtcaggcc tctatattgc agaacttgtg
    11821 aagcggttat ataatagcaa tggcttgaaa gaggcctttc caaatcctga agaacgctta
    11881 aaacatattt tggaaaagca agtttatgga tttgctccgt ctgagattat ctataacatt
    11941 tccactaatt ttatatttgg caatctttct aaagatatca gtaggaagaa ttttgtttta
    12001 gcagatacca ttccagcggc taaagaaggg agcattcaaa agttggttga ttcctatttt
    12061 gaaaataatt aaaaagaagg ccgagtcaaa attctttgaa atcagaaaaa acgcataata
    12121 ttgagtgctt ttgtactgcc ccccaaaagt tagacagaaa aaatctaact tttggggggc
    12181 agttcagaca atccttggta ttatgcgttt tattgtggga agatgtataa tggattgaaa
    12241 taagatatga acaaatcgat taggaattta aagcatttta taacaacgtt ttagagtaac
    12301 ggggtactat ttcatataat acaggaaaaa acaactccct gatgattcaa ggagttgtct
    12361 atagttaaat tagtttttag aagcttcttg gaattctgga tttttccatg cttcgtcaat
    12421 aatagcttgc aattctttag cagatgcttg cattttttga gtttctgcat cgttcaatgg
    12481 aatgtttact ggacgaacga taccatgtgc accaacaaca gctggttgac cgataaagac
    12541 attctcaact ccgtattgac cttcttggaa tactgaaagt ggaagtactg cgttttcatc
    12601 gtcaaggatt gctttagtga tacgagcaag ggctactgcg ataccgtagt atgttgcacc
    12661 ttttttgttg atgattgtgt aggctgcatc acgaacacct tcgaacaatt caatcaattc
    12721 agcttcttga acattttgag tgtctttaag gaattcttca aggtttacac cagcgatgtt
    12781 agcgtgtgac caaacagcga actcagagtc accgtgttca cccatgatgt aggcgtgcac
    12841 tgaacgagca tccacatcca atttttcagc aagtgcttga cggaaacgag ctgagtcaag
    12901 tgaagtacct gaaccgataa cgcgttcttt agggaaacca gagaatttcc aagttgagta
    12961 agtcaaaacg tcaactgggt tagcagcaac aaggaagata cctttgaaac cagattcaac
    13021 aacttgagtt acgattgatt tgttgatagc aaggttttta cttacaaggt caagacgagt
    13081 ttcacctggt ttttgaggtg cacctgcagt gatcacaaca aggtcagcgt ctgcacagtc
    13141 agagtattga gctgcataga tttttttagg tgaagtgaag gcaagggcgt gactaaggtc
    13201 aagcgcatca ccaacagctt tttcatgcaa ttgtggaatt tcgataattc caagctcttg
    13261 tgcaattcct tggttaacaa gtgcaaaagc gtaagatgaa cctacagcac catcaccgac
    13321 aaggataact tttttgtgtt gtttagttga agtcattgtt ttaaacatct ccttaatttt
    13381 attaggggat tttccctaga caacttcatt ctatcacttt taaaaaactt tgtcacgaat
    13441 atgccttata gttctcgatg taaacgtttt agtggtttag aggctgaaat agatgggaat
    13501 ttatggtata atgttgttac ttactaattg tgaaatgagg catttattaa tgcaggataa
    13561 aaatttagtg aatgtcaatc tgacaaagga gatgaaggca agttttatcg actacgccat
    13621 gagtgttatc gtagcgcgag ctcttcctga tgttcgagat ggcttaaaac ctgttcaccg
    13681 tcgcattctc tacggaatga atgaattggg tgtgacccca gacaaacccc ataaaaaatc
    13741 tgctcgtatt acaggggatg tcatgggtaa atatcaccca cacggggatt cctctattta
    13801 tgaagccatg gtccgtatgg ctcaatggtg gagctaccgt tacatgcttg tagatggtca
    13861 tgggaatttt ggttccatgg atggagatag tgctgccgct caacgttata ccgaggcacg
    13921 tatgagcaag attgctctgg aaatgcttcg tgatatcaac aaaaatacag ttgatttcgt
    13981 tgataactat gatgccaatg aacgggaacc cttggtcttg ccagcgcgtt ttccaaacct
    14041 tttggttaat ggagcaactg gtatcgcggt tgggatggca accaatattc cacctcataa
    14101 tctgggtgaa accattgatg cagtgaagtt ggtcatggat aatcctgaag tgactaccaa
    14161 ggacttgatg gaagtcttgc ctggaccaga ttttccaact ggtgctcttg tcatggggaa
    14221 atcaggtatc cataaggctt atgaaacagg taaaggttcg attgtcctac gttctcgtac
    14281 agagattgaa acgactaaga ctggtcgtga gcgtatcgtt gtaacagaat ttccttatat
    14341 ggtcaataaa accaaggtgc atgagcatat tgttcgcttg gttcaggaaa aacgcattga
    14401 gggtatcaca gcagtacgtg atgagtcaaa ccgtgaaggt gttcgatttg ttattgaagt
    14461 caagcgcgac gcctcagcca atgttattct caataacctc ttcaaaatga cccaaatgca
    14521 aaccaatttt ggtttcaata tgctcgctat ccaaaatggt ataccgaaaa ttttgtctct
    14581 tcgtcagatt ttggatgctt atatcgagca ccaaaaagaa gtggttgttc gtcgtacacg
    14641 ttttgataag gaaaaagcgg aagcgcgcgc tcatatctta gaaggtctct tgattgcgct
    14701 agaccatatc gacgaagtga ttcgtatcat ccgtgctagt gaaacggatg cggaagctca
    14761 agctgagttg atgagcaagt ttaagctttc tgaacgtcaa agtcaagcta tccttgatat
    14821 gcgtcttcgt cgtttgacag gtttggaacg cgataagatt caatctgagt atgatgacct
    14881 cttggctctg attgcggatt tagcagatat tcttgctaag cctgaacgtg tttctcaaat
    14941 tatcaaagac gaattggatg aagttaaacg taaattttct gataagcgcc gtacagagtt
    15001 gatgattgga caggtcttga gtctcgagga tgaggacttg attgaagaat cggatgtctt
    15061 gattaccctt tctaacagag gctacattaa gcgtttggat caggacgagt tcactgctca
    15121 aaaacgtggg ggtcgtggtg tccaaggaac gggagtgaaa gatgatgact ttgttcgtga
    15181 gttagtgtca actagcaccc atgatcatct gctcttcttc acaaacaagg gacgtgtcta
    15241 tcgtcttaaa ggttatgaaa ttcctgagta tggtcggact gccaaagggt taccagtagt
    15301 caatctcttg aaattggatg aagacgaaag tattcagacg gttatcaatg ttgagtctga
    15361 tcgcagtgat gatgcttatc tcttctttac aacccgtcac ggtattgtga agagaactag
    15421 tgttaaggag tttgccaata ttcgtcaaaa tggtctcaaa gcgctgaatt taaaggatga
    15481 agatgagtta atcaatgtct tgttggcaga aggagatatg gatattatca ttggtaccaa
    15541 gtttggttat gcagttcgct ttaatcaatc agccgttcgt ggtatgagcc gtatcgccac
    15601 tggtgtgaaa ggggttaacc ttcgtgaagg agacacagtt gttggtgcca gcttgattac
    15661 tgatcaagat gaggttctta ttatcacaga aaaaggatat ggtaagcgta cagtcgctac
    15721 tgaataccca acaaaaggtc gtggtggtaa gggaatgcag acagctaaaa ttaccgaaaa
    15781 aaatggcttg ctggccggtc ttatgactgt tcaaggggat gaggatttga tgattatcac
    15841 tgatacaggt gtcatgattc gaaccaatct tgccaatatt tcacaaacag gacgtgcaac
    15901 tatgggagtt aaagtaatgc gcctggatca agatgctcag atagtgactt tcacaacggt
    15961 tgcggtggca gaaaaagaag aagttgggac agaaaacgaa acagaaggtg aagcataatg
    16021 tctcaaaaaa ataataaaaa gaaaaacaag cgaaaaaatc tgctgacaaa tatcctagca
    16081 ggatttctga tattactgtc actggctttg atttttaata ctcaaattcg aaatattttc
    16141 atagtctgga ataccaataa gtatcaagtt agccaggtat caaaagaaaa attagaagaa
    16201 aatcaggata cagaaggcaa ttttgacttt gattctgtca aagctatctc ttcggaagct
    16261 gttctaactt ctcaatggga tgctcaaaaa ttaccagtta ttgggggaat tgcaattcct
    16321 gaattggaaa tgaatttgcc gatttttaaa ggacttgata atgttaatct cttctacgga
    16381 gctggtacaa tgaaacgcga gcaagtaatg ggagaaggaa attatagtct agctagtcac
    16441 catatctttg gtgttgataa tgctaataaa atgttatttt ctcctttaga taatgctaaa
    16501 aatggcatga agatttatct aaccgataaa aataaagttt atacttatga aatacgtgaa
    16561 gtcaaacgtg tgacaccgga tcgtgttgat gaagttgatg atagagatgg ggtcaatgaa
    16621 atcacattag taacctgtga agaccttgct gctacagaac gtattattgt caaaggtgat
    16681 ttgaaagaaa caaaagatta ttcacaaaca tctgatgaaa ttctaacagc tttcaatcaa
    16741 ccatataaac aattttatta atacaaatca gtgaaatcat ggatttcact gatttttt
//
LOCUS       DAVGJJ010000038        16567 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000038 DAVGJJ010000000
VERSION     DAVGJJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.038"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(66..299)
                     /locus_tag="VVE35_001152"
     CDS             complement(66..299)
                     /locus_tag="VVE35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU3061352.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(305..1297)
                     /gene="plsX"
                     /locus_tag="VVE35_001153"
     CDS             complement(305..1297)
                     /gene="plsX"
                     /locus_tag="VVE35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU3061353.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1294..2064)
                     /gene="recO"
                     /locus_tag="VVE35_001154"
     CDS             complement(1294..2064)
                     /gene="recO"
                     /locus_tag="VVE35_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU3061354.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2061..3230)
                     /locus_tag="VVE35_001155"
     CDS             complement(2061..3230)
                     /locus_tag="VVE35_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3061355.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKVVILNYPANPTGITYSREQLETLAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3379..4389
                     /locus_tag="VVE35_001156"
     CDS             3379..4389
                     /locus_tag="VVE35_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU3061356.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITIFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4502..4582)
                     /locus_tag="VVE35_001157"
                     /pseudo
     CDS             complement(4502..4582)
                     /locus_tag="VVE35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4617..5054)
                     /locus_tag="VVE35_001158"
     CDS             complement(4617..5054)
                     /locus_tag="VVE35_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU3061357.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLKAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5139..7772)
                     /gene="polA"
                     /locus_tag="VVE35_001159"
     CDS             complement(5139..7772)
                     /gene="polA"
                     /locus_tag="VVE35_001159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU3061358.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHIELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            8028..8378
                     /locus_tag="VVE35_001160"
     CDS             8028..8378
                     /locus_tag="VVE35_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU3061359.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            8593..8709
                     /locus_tag="VVE35_001161"
     CDS             8593..8709
                     /locus_tag="VVE35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU3061360.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            8702..8935
                     /locus_tag="VVE35_001162"
     CDS             8702..8935
                     /locus_tag="VVE35_001162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061361.1"
                     /translation="MFSQLHMREEQVLLAQDYPLETARAEGLEQGLERGKVEGREERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            9089..9367
                     /locus_tag="VVE35_001163"
                     /pseudo
     CDS             9089..9367
                     /locus_tag="VVE35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9504..10472)
                     /locus_tag="VVE35_001164"
     CDS             complement(9504..10472)
                     /locus_tag="VVE35_001164"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU3061362.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(10617..11159)
                     /locus_tag="VVE35_001165"
     CDS             complement(10617..11159)
                     /locus_tag="VVE35_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU3061363.1"
                     /translation="MDQLLGVLFPDSTFLNAFESAIVAPLVEEPLKLLPLVFVLALIP
                     VRKLKSLFLLGIASGLGFQMIEDIGYIRTDLPEGFDFTISRILERIISGIASHWTFSG
                     LAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAIPVVTAI
                     ALYGFYHAYCFVEKHNELMT"
     gene            complement(11163..11447)
                     /locus_tag="VVE35_001166"
     CDS             complement(11163..11447)
                     /locus_tag="VVE35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061364.1"
                     /translation="MKKSSILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFLALFYM
                     IPALYFLFHIGKKWELPKKVLILSLLGAICSFTSLLLFGIYNHRRKSSKV"
     gene            complement(11472..11969)
                     /locus_tag="VVE35_001167"
     CDS             complement(11472..11969)
                     /locus_tag="VVE35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU3061365.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(12042..13403)
                     /gene="radA"
                     /locus_tag="VVE35_001168"
     CDS             complement(12042..13403)
                     /gene="radA"
                     /locus_tag="VVE35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU3061366.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(13417..13932)
                     /locus_tag="VVE35_001169"
     CDS             complement(13417..13932)
                     /locus_tag="VVE35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3061367.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(13934..14377)
                     /locus_tag="VVE35_001170"
     CDS             complement(13934..14377)
                     /locus_tag="VVE35_001170"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU3061368.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(14437..14530)
                     /gene="ffs"
                     /locus_tag="VVE35_001171"
     ncRNA           complement(14437..14530)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVE35_001171"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(14563..15030)
                     /locus_tag="VVE35_001172"
     CDS             complement(14563..15030)
                     /locus_tag="VVE35_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU3061369.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(15231..16517)
                     /locus_tag="VVE35_001173"
     CDS             complement(15231..16517)
                     /locus_tag="VVE35_001173"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU3061370.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT         4899 a         4123 c         2941 g         4604 t
ORIGIN      
        1 ccagcttgtc cgatatttct catttctaaa aaccatctac ttccgttgac ttggaactcc
       61 gattgctatt ttccttgaat gattttaacc acatctccta cgttttggag ttggtcaatt
      121 tcttcatcgc tgatttcgat actaaattca tcttccagag tcaagataaa ctccatcaag
      181 tcaacagaat ccgcatccaa atcgtctttc agactcaagg attctgtcac gacaaagtcc
      241 tctccctgtc gctcttggat aatggtcaca atacggtcaa aaatttcttt ttctgtcatc
      301 tcttttattc tcctgaaaat tcacgcgcag tctgggcaac tacgtctgtt tctagcatgg
      361 tacgaatctg gcgaatcgta ctataaacag ccttggcatc gcttgagcca tgagtcttga
      421 caacaggtgc cttaacacca aacaagaccg ctccaccaac atctgaataa ttgagctgtt
      481 ttttcaaacc tctgaggctg tccttgagaa ggagggcacc tagtttcgct cgaagaccac
      541 cacctgtaat agctgtcttg agcaagccca tgattcccat agctgtccct tcgatggatt
      601 tgagcacagc gtttcccgtg aaaccatctg ccacaacaac atctgcaacg ccattcatca
      661 aatcacgcgc ttccacattt ccgataaagt tcaaactttc atcagccact agtaattcat
      721 aagtttcctt acgaagcgga tcgcccttgc tactctctgt tccgttattg agcaagccaa
      781 cacgtggttg cgcaatgcca cgaacatttt tagcatagaa ggatcctaga accgcgtatt
      841 gatggaggtg ctgggctgta ttttccgcat tggcaccgag gtctagcatg tcaaatccct
      901 tcccatctac agtcggcaaa gtcgacataa gcccaggacg gtcgatattc ttgatacgac
      961 ccacgatgaa gaatccagca gccaacaagg cacctgtatt cccagccgaa aggacagcgt
     1021 ctgcttcacc atctttgaca gccttggctg ccaataccat actggcattt ttcttattcc
     1081 taatagctct cgtaggttca tcatccgaat caatcttctc atccgtatgg ataatgctga
     1141 cgcgctctgt cgctgtcaga tattgcttga ttttagcttc atctccgtaa agttgaacct
     1201 cgatatctga aaagtcagat agggcttgat tgacaccctc aacaatggcc tgaggtgcgt
     1261 aatcgccccc catggcatct actgcgattt ttttcatttc ttttcctctt ttagtaattg
     1321 tccccagtct gctagggaat caataaattt ctttgatttt aggtgaatcc caacgtactc
     1381 ttcatataat tgatccataa attggcgtag ctcttgcttg attccaggct tgagcgaaat
     1441 ggtctccaaa gtctcaaaat caatagcttg aaattgattg agcagatagg ggatattggg
     1501 attgagatga caacgtctct tatcctcatg ataatgctct ggacagaggc aggctccata
     1561 tttgaaagaa aagtcaaaag cctgaccaac ccgatggcag aagacacact cattaaaatt
     1621 gaggctgatt ccaaatcgag tcaaaatttg aatttcaaaa atattggtca aaacctgata
     1681 atccaagcct gcttccatca actccaaagt cttttgcaaa aaagcaaaca agggagcatc
     1741 ctgctgattg tcctgcaaac tagcatctgc aagagctgcc acataggtcg cataggccat
     1801 gacaaagagg tcactattaa tcttgggaaa agtcatgacc tcatgatagt cttcgatgta
     1861 actgagtccg tcatcattga ttcgcaagag aaatcgtgcc agcaccaagg gctgaataac
     1921 aggcgccagc ttagactgac cagcgtgttt gacaaaaaac atgcgtttgc caacctgctc
     1981 tgtaaaaatt ttgacgagct tgtcatcctc acgaaaattg cgattgtaaa gcaccaagcc
     2041 ttgactcgtg atagactgaa tcatgcttct ctcatgtact cctcaagtcg tttcatggct
     2101 tctttgatag tctccatgct ggctgcataa gataggcgga cgtagccttc cccgtaacgt
     2161 ccaaaggctg caccagggat aaaggcaacg gccttcttct gagcaaaatc cttcagaaaa
     2221 gcaaaggagt cttgattgta gcccgctgga attttagcaa aaatatagaa ggcaccgtct
     2281 ggtttgataa tctcaaaacc aagagcagtc attttttcga tgatatagtc ccgacgttgg
     2341 atatattcct tcttcatggg ctccgcatcg tttttaccag ccgtcaaggc ttctaccgca
     2401 gcatgttgcg ccatggtatt tgcggcagtg accaagtact ggtgactctt gattaactgg
     2461 gctgtgaagg tcgcaggagc gaaaatcagc cccaaacgcc aacctgtcat ggcatgcgat
     2521 ttagacaaac cattgataat aatagcctgg tctctcaaca tcgttcctag agacacatgg
     2581 gcttcgcctg tgtaggtcaa ttctgagtaa acctcatcac agacaacaaa aatttcatac
     2641 ttgcgtaaaa cagctgccaa ggtctctaac tgctctcgac tgtaggtaat tcctgtcgga
     2701 ttggctggat agttgagaat aaccaccttg agcttatcac cctgctccaa aatggccttc
     2761 tccaacatct caggagtcaa gacaaaacca ttttcagtcg tatcaatctc aacaatttct
     2821 gccccaacta agttaacaat cggttcatag cctggataag caggagctgg caaaagtacc
     2881 ttgtctccct cttccaaaat agccgtcaaa gtcgcagata aagcctctgt cgccccaatt
     2941 gtaaccaaga tttcattttc aggagcatag tctagttggt acttttcctt aacaaagtca
     3001 ctagctgcct gacgtagagt cagcagacca ctcatccctg tatagtagga ttggttctga
     3061 tcaatcgctc gcttggccgc ctccttgaca tggtctggcg ttgtaaaatc aggttccccc
     3121 aaggtcaaac gcaagacccc aggaatctcc gaaatagcct ggtcaaactg acgaatcaac
     3181 gaaacttgaa ttttatctaa ctgtttatta aagcgcttag ttaagtccat agatacctcc
     3241 tactttcaaa acgtatctct attatactac aaaagcctcc cgaccgcaaa aatacattag
     3301 agaatagctt tccactttct attttacttt tcctctttac aggtctataa tggtaacaga
     3361 ttctatttgg aggtttttat gtcatttcta tcaaaaaatg gagcaggcat cttggtctgc
     3421 cttctcattt ctatcgtatc ttggtactta ggaggattct tccctgtgat tggcgcaccc
     3481 gtttttgcga tttttatggg catgctccta catccctttc tctcgtctta taaacaactg
     3541 gatgctggat tgacctttag ttccaagaaa ttgctccagt atgccgttat cttgcttggt
     3601 tttggtctca atatctctca agtcttcgca gttgggcaat cttcactccc tgtcatcctt
     3661 tccaccattt caatagcctt gattgttgcc tacctcttcc agcgcttctt tgccctggat
     3721 acaaaactgg ctaccttgat tggagtaggt tcttctatct gtgggggttc tgccattgca
     3781 gcgacagcgc ctgttatcca tgctaaggaa aaggaagtag cccaagccat ctccgttatc
     3841 tttttcttca atgtcttggc tgcgctcatc tttccaaccc tcggcacctg gcttcatcta
     3901 tccaatgacg gcttcgccct ctttgcagga actgcggtca atgatacttc ctctgtaacc
     3961 gccacagcta gcgcttggga cagtctctac cagaccaata ccctcgaatc cgcaaccatt
     4021 gtcaaactca cgcgcaccct agctatcatc cccatcacta tctttctctc ctactggcaa
     4081 agtcgcgagc aaaaaaataa gcaaggctta caactgaaaa aagtcttccc actttttatc
     4141 ctttacttca tccttgcatc gctcctaacc acccttctga cctctctcgg tgtgtctagt
     4201 agtttcttta cccctctcaa acaactctct aaattcctca ttatcatggc catgagtgcc
     4261 atcggtctca aaactaatct ggtcgctatg gtcaaatcca gtggaaaatc cattgttctc
     4321 ggagccgtct gctggattgc catcatcctc accagccttg gtatgcagac ccttatcggc
     4381 attttctaat actcttcgaa aatctcttca aaccacgtca acgtcgcctt gccgtatata
     4441 tgttactgac ttcgtcagtt ctatctgcaa cctcaaaacg gtgttttgag ctgacttcgt
     4501 cagtcgtatc tacaacctca aaacagtgtt ttgagcagcc cgcggctagt ttcctagttt
     4561 gctctttgat tttcattgag tataacacaa aaggtagccc atcagctacc tttttcttat
     4621 gcttcctcaa tcaagcgagt atgttctctc ttgatacagc gattcatcac gatatcatca
     4681 catccaccat cacgcaaaat ctctttcgct tttaaacttt caagtcctag ctgtgcccaa
     4741 aaaatcttgg catcagcttt gagaaaatca cgcgccacat cgggcagaaa ttcactgcga
     4801 cgataaacat tgacaatatc tacaggaaaa ggaatttcag cgaggctagc ataagccttt
     4861 tcacccaaga tttcgccacc tgccgccttg ggattgactg ggatgatttt atagccccga
     4921 gcctgcattt cctttgtcac tcgattgctg gttgtttctt cacggtcaga caaacccacc
     4981 acagcaaggg ttttactcgt tgcgagatac tgacgaatca cgccatcact tggattgata
     5041 aattcttgac tcatagaaat cctccttttt catcagtata gcacattttg aaaaggtttg
     5101 cagaattcta ctacaaaaaa ggaggactag cccccttttt atttagcctc gtaccaggtt
     5161 gccccttcat tctcatctgc gataagagga acactgagtt gaatggcttc ttccatggtt
     5221 tgtttcacca attttttcat ctctaccaat tcagatttag gcacttcaag gacgatttca
     5281 tcgtgcactt gtaacagcat cttagtctga taaccacctg caaccaaggc tttatccagc
     5341 tgaatcatgg caatcttgag aatatctgct gccgaaccct ggataggtga gttgatagca
     5401 gttcgctccg caaaaccacg aatattgaag ttgcgcgaat tgatatctgg caactcacgg
     5461 cgacgcttaa agagggtctc tacatagccc ttatcacgcg cctcccgcac cacttcatcc
     5521 atgtagtttt taatacctgg aaaacgttca aagtaggtat caatgtaggc tttggcttcc
     5581 ttacgactaa ttcccaaatt attagacaag ccaaagtctg aaatcccata aaccactcca
     5641 aagttaactg ccttggcatt gcgacggtca tttgcagtca cattatcagg acgctcaatg
     5701 ccaaagaccc gcatggctgt cgaagtatgg atatctgccc cctcttggaa ggccttaatc
     5761 aagtgctcat ccttagaaat atgcgccaaa acgcgcaatt caatctgtga atagtcagag
     5821 ctgagtagca cactatcctc ccactctggc acaaaagcct tccgaatcaa gcgcccttgt
     5881 tccaatcggg caggaatatt ttgcaagttt ggatccacac tagacaaacg cccggtctgg
     5941 gtcaaatcct gcatatagcg agtatgaatc tttccatcag ccaaaatcca gtcctgcaag
     6001 ccaattacat aagtagattg aatcttagca atttgacggt aatccaggat tttcttaaca
     6061 atcggagcaa taggagcgag acgctccaac acatccactg ctgtcgaata acctgtcttg
     6121 gttttcttag tgtattctag aggaagtccc aatttctcaa agagaagcac gcccaactgc
     6181 ttaggcgagt tgacattaaa ctcctcacca gccagctcgt aaatctcttg agtcagtttt
     6241 tcaatgacaa gctcattttc agcctgcatc tcaagcaagg tctctttctt gaccacaatc
     6301 ccagcaattt ccatcttggc aagaacaaaa gccagaggtt gctccatatc ataaagaagc
     6361 tctaattgcc cattttcgct gagtttttca agtaaaatag gctctgtttc taccaaaaca
     6421 gcaagtttac aagctaagtg ttccaagaat ttctcacgtt caggaatggc ctttttaaca
     6481 cccttaccgt agaaagtttc atcatcaacc aagtaagtct gaccataaag actagcgatg
     6541 gtcgcaattt cattgtcctc cacagtcgaa aggaggtatt tagccaaacg gatgtcaaaa
     6601 gcaggcgcct gcaaatccac accaaaacgt tgcaaaagaa ctttaacctt cttaaagtca
     6661 taaactctca gagatgtttt ttctaagaaa tccttgaaaa tcgggtcttg caacagctca
     6721 agcttgtctg tggcatagag cttatcccca caagaccagg caaatccaac caaattatcc
     6781 gtatggtaat tctcaccaaa aagctcaatg tggaagatag actcttcact cagcatatct
     6841 tgactgattt ggtcaacaat agtaaaatcc aaaccctcag ccacatcagc tgacgacaca
     6901 tttaaagcct gcttgagctg tttgaagccc atctcatcgt aaaatttccc aagattttca
     6961 acatctggac cactatagac caagtcctct aaaccaatcg caatcggtgc cttggtatca
     7021 atggtcgcta gtgttttaga caaaaaggcc tgttccttgt cattgatgag attttccttc
     7081 atcttagaag tcttcattcc atcaatattt tcataaatcc cctcaagcga accatgctcc
     7141 agcaagagct taatacccgt cttttcaccg actttggtca ccccagggat attatccgac
     7201 ttatcaccca tgagcgcctt gagatcgata aactgagctg gtgtgaggcc catttcttcc
     7261 atgaggtaat ctggcgtaaa ggcctcaaac tcagccacac ctttcttgga aatttcaacc
     7321 accgtatgct catccgtcag ctgaatcaaa tccttgtccc cactgacaat ggtaatatca
     7381 aaaccatcct gctctgctag cttatccagc gtcccaatga tgtcatccgc ctcatactga
     7441 gccagctcat agtgacgaat ccccatatga tccagcaact cacgaatgaa aggaaattgc
     7501 tcacgaaact catcaggagt cttggcccga ccacccttat agtccgcata catctctgtc
     7561 cggaaggtcg tctttcccgc atcaaaagcc accaaaatat gactcggctc aacccgctcc
     7621 aacaaatgac tcaacatcaa ctggaaacca taaatcgcat tggtatgcaa accagccgca
     7681 ttcttaaaac ggtccaactg ctgatacagc gcaaaaaacg cccgaaaagc tacagaagac
     7741 ccatcaatca ataataattt tttcttatcc atacacccat tataaaggaa agaatcaaaa
     7801 aataccattg ggaagagcta gagcaagtat ttttcaaact ttttccgaat aaatagatag
     7861 agccagagaa tttagtaaac ctagatttaa aaatgtgcta taacatagta tattgaatct
     7921 ataatagtac accttgactg ctaaaatatt tctataaatt aatttgactt tcctgataga
     7981 gttattcaca tcttatttca actcactata gaaggaggaa taggaggatt ctcagacatc
     8041 cgggcatcag cccaactaat gatttgattg ctaagaaaat attcagcaat ccagaaatca
     8101 cttgtcaatt tattcgcgat atgctggact tgccagcaaa aaatgtgacc attttggagg
     8161 gaagcgatat tcacgtatta ctctccatgc cttactcagt gcaggatttt tataccagta
     8221 tagacgtctt ggcggagttg gataacggta ctcaagtaat tattgagatt caagtccatc
     8281 atcagaattt tttcatcaat cacttgtggg cttacctgtg cagtcaggtt aatcaaaatc
     8341 ttgaaaaaat tcgtcagcga gaaggtgata ctcactagag ctacaaacac atcgctcctg
     8401 tttacgccat tgctatcgtg gatagtaatt atttctcaga tgacctggct tttcatagct
     8461 ttagtatgcg cgaagacaca acaggtgagg tattggcgat taccaacaat ggacaggaaa
     8521 accatctggt taagatggca ttcttggaat taaaaaatac agagaaacca gcaaagacaa
     8581 ggttcgcaag ccatggttgg agtttttcgg caacaagccc tttacccagc aaccgcaacg
     8641 agccattacc caagcaaatc aactgctgga ctacaagagc tggtccgagg aggacaggaa
     8701 aatgtttagt caactacata tgcgagaaga acaagtcttg ttagcacagg actatccctt
     8761 ggaaactgct agggctgaag gccttgaaca aggactagag cgtgggaaag ttgaaggaag
     8821 ggaagaaagg aaactttttg ccttcctaga catagtacgc caaggtcttc tgacttctga
     8881 ggttgccagc cagcaattag gtatgtcagt atctgaattt gaggcactgt tgtaaaatgg
     8941 ctccataata tccatagtgg gtaaatcggc tctataatat ttgtagtggg taaatcccct
     9001 atggatatta tggagcctat tgtgtagaaa aaaagtccca taagatctat aatgaaaagc
     9061 gacaaaacaa ctcattagaa agaatcatat ggaacaatta cattctatca caaaattact
     9121 agacattaaa gaccctaata tccagatttt agacatcatc aataaagata cacacaagga
     9181 aatcatcgcc aaactggact acgacgcccc atcttgccct gagtgcggaa accaattgaa
     9241 gaaatatgac tttcaaaaac cttctaaaat tccttatctt gaaacgactg gtatacctac
     9301 tagaattctc cttagaaagc gtcgattcaa gtgctatcac tgttcaaaaa tgatggtcgc
     9361 tgaaacttat gatgacgtac agtcatattt cttctctttt tattatatca cagttttaaa
     9421 tctagcttta ctagattcac cgctactatc tatttattcg gaaaaaagac gaaaaaacct
     9481 gagaatcatc tcaggcttgg tcattaaatt tttttctcaa tatcgaaaag tggagaaagt
     9541 ggtcgttttt catgaatacg tacgatagca tcccctagga gatgagcgat tgaaatctgc
     9601 tcaatcttat caatcaaacg ctcttctggc agatagatgg tatccaaaac aaccaatttc
     9661 ttaatagctg atttttggat attgtccgta gcaggaccag aaagaactgg gtgcgtacag
     9721 cttgcataga cttcaacagc accagcttcc gcaagagcat ctgccgcatg acaaatcgtt
     9781 ccagcggtat caatcatatc atcaatcaag atacaagtct tgccttcaac cttaccgatg
     9841 atgttcataa cttcactagt attcatctta tcaacgctac gacgtttatc aataatagcg
     9901 atagatgttt tcaaaaattc tgccaactta cgagcacgag tcacccctcc atggtccggg
     9961 ctgacaacca catagtcaga accaaccata ccacgacgct caaaataatc tgcaatcaga
    10021 ggagcaccca tcaaatgatc cacaggaata tcaaagaatc cttgaatttg cgcagcatgc
    10081 aagtcgatgg tcaataaacg atccactcca gctacttcaa gcatatttgc gacaagtttt
    10141 gaagtgattg gctcacgcgc tctcgccttt ctatcctgac gtgcataccc atagtaaggc
    10201 atgacaacat tgacagattc tgcactcgca cgcttcaaag catctaccat aatcaaaatt
    10261 tcaagcagat tgtcatttac aggcgaacta gttgattgta agataaagac gtgtttccca
    10321 cggattgatt cttcaatgtt gacctgaatc tctccatctg aaaattggcg aacacttgat
    10381 ttccccaact ctatcccaat ctcctgcgcc acacgttctg ccaattcttt attagaagaa
    10441 agggcaaaca gctttaaatc agaaaaagac atgatttcct ccggtatata tgtataactt
    10501 gtgcttttca caagattttc catctaccat tgtagcgctt tttgcactat ttttcaatca
    10561 aaaataaaag aagggcacca tatttgtacc cttgcatcat tcttttgaaa aatattctag
    10621 gtcatcaact cattgtgttt ctcaacaaag caataagcat gataaaaacc atagagagca
    10681 atagccgtaa ccactggaat cgctaaaggc aactctgttt ccaactccac aaaaggagag
    10741 ttaaacaaga agtgagttcc caaggctaaa cctagaaaaa taaggccctg tttcttgcca
    10801 accttctgtc ctttataggc tctgtaaagc aagtaaacac ctactacagc tagacctgaa
    10861 aaagtccagt gagaggcaat tcctgagatg atacgctcta aaattcgcga aatagtaaag
    10921 tcaaagccct ctggcaaatc cgtacgaata taaccaatat cctcaatcat ttggaatccc
    10981 aaaccggaag caattccaag taaaaacaaa gattttaatt ttcgcacagg aatcaaagcc
    11041 aaaacaaaaa caagtggcaa taatttcaag ggttcttcta ccaaaggagc cacaatagca
    11101 ctttcaaagg catttaaaaa tgtactatct gggaaaagaa cccccagtaa ctggtcgatt
    11161 ttttatacct tagatgactt tcgtctgtga ttatagattc caaatagtaa gagagaagta
    11221 aaggaacaga ttgctcccaa taaagacaga atcaaaacct tctttggcaa ttcccatttt
    11281 ttcccaatat ggaagagaaa ataaagagcc ggaatcatgt aaaagagagc tagaaagata
    11341 gaaactccca ttagtccata ttccgcacct gacctcgaac cgtccgtata gtagatggtt
    11401 tcatactgta aaccaataca tagcaataaa atactgcttt tcttcataca ctttctttct
    11461 aaatgaagta tttataattc tacgactgtc atacttcctg tatcaacatt gtaaatagca
    11521 ccagagataa tgacatcgtc tggtattagg ggagactcga taagcagttg catatcctcg
    11581 cgtacactct cttctatatc ttggaagggc aagaagtcct ggtctgacac atctacacct
    11641 aattcctctt ttaaatactc ctgaaaaggt tcattttcaa aggtctgagc accacagtct
    11701 gtatggtgca ataccacaat ctctcttgtc cccatttgtt gctgggaaat aactagcgaa
    11761 cgaatcatgt cttcagtcac tcgaccacct gcattccgca agatatgagc atccccaagt
    11821 gccaagccca gagcttgcgc aacgtgcaga cgagagtcca tacaggtcac aatagctact
    11881 cttgttttgg gtttaagtgg cagatttaac tgcccatgta gggcaacata agcctgatta
    11941 gcttgcataa actgttcaaa atacgacacg attccctcct tgaaaatttg atagtcaaat
    12001 atttctccta tcttatcatt tttaagagaa tttgtcacgg attatgcaaa gacctttttc
    12061 aaaacttcct gaatcgttgt cacgccaatg acctgaattt ccttaggcag agtgattcct
    12121 gtcaaggaat tcttaggtac ataaatctta gtaaagccca gtttagcagc ttcgttgatg
    12181 cgttgctcaa tacgattcac gcgccgaatc tctcctgtca agcccagttc tccgacaaaa
    12241 cattcctgag gattagttgg cttgtctttg tagctcgaag caatagcaac tgcaacagcc
    12301 aagtcaatcg caggttcatc caatttaaca ccaccagcag atttgagata ggcatcctga
    12361 ttttgcaaga gaagccctgc ccgtttttcc aaaacagcca taatcaagct agcacggtta
    12421 aaatcaagtc ctgtcgtagt acgcttggca tttccaaaca tggtcggtgt taccaaagcc
    12481 tgaacctccg ccaaaatcgg acgcgtccct tccatggtta caacgatgga ggaaccagtc
    12541 gccccatcca aacgctcttc taggaaaact tgactcggat ttagtacctc aaccaagccg
    12601 cccgactgca tctcaaaaat cccaatctca ttagtggaac caaaacgatt tttgaccgct
    12661 ctcaaaatac gaaaggtgtg gtgacgctcc ccttcaaagt aaagcaccgt atccaccata
    12721 tgctccaaca tacgaggccc agccaaggtt ccttctttgg tcacatgacc tacgataaag
    12781 atggcaatgt tattggtctt ggccaactgc atgagttcag cggtcacttc acgcacctga
    12841 gaaacagacc cctgcacccc tgaaatctca ggagacatga tggtctggat ggaatcaata
    12901 atgagaaagt ctggctggat acgctccact tctgcacgaa cactctgcat attggtctct
    12961 gcatagagat aaaactcact atcaatatca cctaagcgct ctgcacgtag tttaatctgc
    13021 tgggcagact cctccccact gacatagaga actgtcccca cttgggacaa ctgggttgag
    13081 acttgtagga gaagggttga tttcccaatc ccaggatccc caccgatgag gacgagactt
    13141 cctggtacca ctccgcctcc aagcacacgg ttgaattcct ccatctccgt cttggttcga
    13201 ttgacattga tggaagtcac ctcagctagt ttcatgggct tggttttctc acctgtcaag
    13261 gacacacgcg cattcttgac ctcggcaacc tcaacctctt ccacaaaaga agaccaagac
    13321 ccacagttgg ggcaacgtcc cagatattta ggggaattat acccacaatt ttgacataca
    13381 aatgtcgctt ttttctttgc gatgacaaac ctctttctat atctctaact cacactcaat
    13441 cacttggcaa aaatcaatct tctcatttgg cacaaactgg cgcatgagca ttcgatgagc
    13501 aacaactacc acagtctgat gttctcgata cttagacata cattctagaa accgagactt
    13561 catttccgta gctgtctcat attgaatagg actattagga agcaactccc ccttgttttc
    13621 taaaaacagt cttctagctg tttcaaagtt ttctattcct gttttataga cctgccattc
    13681 atgtaataaa ggctctactc ttaaaggaag acccgtagca cagaccacat acgaagccgt
    13741 ttctaaagct cttgtgactg cagaagatac gattatttca gctgacgaga gtaaaggatt
    13801 tttgctcaat ttctggactt gctgccgtcc catctcagac aagggtgcca aatctatccc
    13861 aaatcctata taagaacgct cctctaactc acggtaatct ggctccccat gacgtacaaa
    13921 gataatcttc attctagtgc cctgtcgatc caaatccacc agttcgaacg ccatcagccg
    13981 catctccatc tgcaattaag aaagtagcaa aaacagcctg gacaatacgc tccccaactt
    14041 caagaacaac ctcttggtct gtgatattct tcatctgcgc aaaaatatgc ccttcatttc
    14101 caggatttcc ataataatct ccatcaatga ccccaactga gttaattaaa accaagccct
    14161 tcttacgagg atttgaagaa cgatcataga ggtagagaac ctcagtcggc tgcatataag
    14221 ccttaacccc tgtcggaacc aagacaatct ctcctggcgc aacaactgta cgcacagcaa
    14281 cctttaagtc gtaaccagcc gcatgcgctg tctcacgctt gggcaataaa ttttcatctg
    14341 taaaactcga aaccaattca aaaccacgaa ttttcataat tttctctttt ctattatcat
    14401 ttattctaga ttattctatc ttatttattc ggaaaaagca cgaaaaaaga gcacacaatt
    14461 caaatcgctt agggctgctg gattcctccc ctgacccgct tcacgcagaa ctgttgctcc
    14521 actatttatt atatcacatt cctattcatt ttaaaagcaa aattattttt tccgtctatt
    14581 tctaaaaaag tcctgcataa tagctgcgca ttcatcttcc aaaattccta tttcaacctc
    14641 cacacgatga ttgagacgct catctgtcaa gatatcgtac aaactcccag cagcgccaaa
    14701 tttctggttt ttagccccat agaccacatt tggaatgcgg gcaagcccaa tcgccccact
    14761 acacatgaca caaggttcaa tggtcacaaa aagtgtgcaa tccagcaagc gccagctctc
    14821 ctcactcaag ttcgcatcct ctatagccat aatttccgca tgcataaccg ctcgctgtaa
    14881 ttcctcacgc gcattatgcc cacgaccaat gatttcccca tctttgacaa tcacacaacc
    14941 aattggaatt tcatcgtgtt caagagcaat ctcagcctct ctcaaagcct ccctcataaa
    15001 gacttctttt tcttcaagcg tataatacat ctcttttctc ttttcctact tatcgatttt
    15061 attattatat catgaatccc aacacaaaaa aagccaccga atgcggtgac tctataggga
    15121 gattattatg aaaaagaaaa gtttaggata tttgttacaa caagttagga ggtcttcttg
    15181 taactgtcta tagtataccc gacctatctt aaacaaatct taaaaatctc ttaggaccaa
    15241 acactttcta aaatatttgt ttgttcacga ccaggaccta ctgagaaagt agaaatacga
    15301 acgccaacca attcactcac acgacgaaca tagttacgcg cattctcagg aagatcttcc
    15361 aaattgcgaa ctccggtaat atcttctgac caacctggca actcttcata gataggcttg
    15421 caacgtttca attgctcaag actagctgga tagtagtcaa tacgttgacc gtcaagatca
    15481 taggccacac agattttcac agtatccaaa ccgctcaaaa catcaataga gttcaaagaa
    15541 aggttagtaa taccagaaac acgacggcta tgacgcatca caactgagtc aaaccaacct
    15601 acacgacgtg gacgaccagt tgttgtacca tactcatgac ccacttcacg gatacgttct
    15661 cccacttcat caaacaactc agttgggaaa ggaccatctc ctacacgact cgtataagct
    15721 ttacatacac ctacaacctt gtcaatcttg cttggaccga caccagaacc aattgtcaca
    15781 ccaccagcta cagggtttga tgacgtaaca aatggataag taccttggtc gatatctagc
    15841 ataacacctt gtgcaccttc aaaaagcaca cgtttgccat tatcaagcgc atcattcaag
    15901 ataacagatg tatctatcac gtatttcttg atttgttgac catattcgta atattcttca
    15961 aaaatatcat cgaaaacaat cgctttactg tcatacaatt tttcaaaaag acgattcttt
    16021 tcagcaaggt tacgttctaa acgctcacgg aaaatatctt tatctaaaag atctgcaata
    16081 cgaattccaa cacgagcagc cttgtccata taagctggac caattccctt aattgtcgta
    16141 ccaatcttat tgtcgccctt agcttcttct tgcaagcgat ccaactcgat atgataaggc
    16201 aaaataacat gcgcacgatc agaaatacgc aagttatcag ttgttacacc ttcctcatga
    16261 agatagctca actcttttac aagagattta ggatttacaa ccataccatt cccaatgaca
    16321 gatatttttt cagggaagaa aatcccagat ggaatcaagt gcaacttaaa tttcttaccg
    16381 tcaatcacaa tcgtgtgacc agcattatca ccaccttggt aacgtgcaat cacttctgca
    16441 ttcgctgaaa ggaagtctgt aatcttccct ttaccttcat caccccattg ggtacctaca
    16501 acaacaactg aagtcataat tttgtctgag ccctcaggct cttcctttct cacatacatg
    16561 gcaggac
//
LOCUS       DAVGJJ010000039        16473 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000039 DAVGJJ010000000
VERSION     DAVGJJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.039"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..270
                     /locus_tag="VVE35_001174"
     CDS             <1..270
                     /locus_tag="VVE35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061371.1"
                     /translation="NHVLTLIFLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDV
                     IAYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELFKNSK"
     gene            1405..1644
                     /locus_tag="VVE35_001175"
     CDS             1405..1644
                     /locus_tag="VVE35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061372.1"
                     /translation="MKLLDCILDYQERFNRKACQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1629..1907
                     /locus_tag="VVE35_001176"
     CDS             1629..1907
                     /locus_tag="VVE35_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061373.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKLTSCCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            2193..4220
                     /gene="pspA"
                     /locus_tag="VVE35_001177"
     CDS             2193..4220
                     /gene="pspA"
                     /locus_tag="VVE35_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU3061374.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSSPTVVRAEEAPQVVEKSSLE
                     KKYEEAKAKYDAAKKDYDEAKKKAAEAQKKYDEDQKKTEDKAKAVKKVDEELQKANLE
                     LQKAHVKYQKAQRELSESPDRRKSAARKKLADALSYIGEVELKQKEAEANFNTEQAKV
                     IPKETELAVTKQKAEEAKKEAEVAKKKFDKAAQEVEVAKKEVEAKELEIEKLQDEIST
                     LEQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKL
                     LDSLDPEGKTQDELDKEAAEAELDKKVESLQNKVADLEKEISNLEILLGGADSEDDTA
                     ALQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPA
                     PKPEQPAPAPKPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYN
                     TDGSMAIGWLQNNGSWYYLNANGAMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWY
                     YVNSNGAMATGWLQYNGSWYYVNSNGDMATGWLQYNGSWYYLNANGDMATGWLQYNGS
                     WYYLNANGDMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDG
                     DTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            4620..5741
                     /gene="mnmA"
                     /locus_tag="VVE35_001178"
     CDS             4620..5741
                     /gene="mnmA"
                     /locus_tag="VVE35_001178"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU3061375.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5882..6337
                     /locus_tag="VVE35_001179"
     CDS             5882..6337
                     /locus_tag="VVE35_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3061376.1"
                     /translation="MTQQDFRTKVDNTVFDVRATALILQNRKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6347..8260
                     /gene="mnmG"
                     /locus_tag="VVE35_001180"
     CDS             6347..8260
                     /gene="mnmG"
                     /locus_tag="VVE35_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU3061377.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(8595..10274)
                     /locus_tag="VVE35_001181"
     CDS             complement(8595..10274)
                     /locus_tag="VVE35_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU3061378.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTVIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10276..10509)
                     /locus_tag="VVE35_001182"
     CDS             complement(10276..10509)
                     /locus_tag="VVE35_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU3061379.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11327..11479)
                     /gene="cibB"
                     /locus_tag="VVE35_001183"
     CDS             complement(11327..11479)
                     /gene="cibB"
                     /locus_tag="VVE35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU3061380.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11481..11666)
                     /gene="cibA"
                     /locus_tag="VVE35_001184"
     CDS             complement(11481..11666)
                     /gene="cibA"
                     /locus_tag="VVE35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU3061381.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11844..12527
                     /gene="tsaB"
                     /locus_tag="VVE35_001185"
     CDS             11844..12527
                     /gene="tsaB"
                     /locus_tag="VVE35_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU3061382.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12524..12961
                     /gene="rimI"
                     /locus_tag="VVE35_001186"
     CDS             12524..12961
                     /gene="rimI"
                     /locus_tag="VVE35_001186"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU3061383.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12951..13961
                     /gene="tsaD"
                     /locus_tag="VVE35_001187"
     CDS             12951..13961
                     /gene="tsaD"
                     /locus_tag="VVE35_001187"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU3061384.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(14001..15307)
                     /locus_tag="VVE35_001188"
                     /pseudo
     CDS             complement(14001..15307)
                     /locus_tag="VVE35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15640..15934)
                     /locus_tag="VVE35_001189"
                     /pseudo
     CDS             complement(15640..15934)
                     /locus_tag="VVE35_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16041..16340)
                     /locus_tag="VVE35_001190"
                     /pseudo
     CDS             complement(16041..16340)
                     /locus_tag="VVE35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4972 a         3088 c         3737 g         4676 t
ORIGIN      
        1 aatcatgtac tgactcttat ctttttgata aaaatttgtg cagataccac gataaaattt
       61 ttattcatca gattatttga cacaagtact ttaatgaaga ggagaatatt ttttcttttc
      121 gcattggatg ttatagctta cttattttta ggatatcttt tagtgattca aaaagcaggc
      181 tatttgtttt cggttttact tcttttttct aatttttcag ttccatttat caaagaaaag
      241 gaattcgaat tatttaaaaa tagcaagtaa tagaaacata ggttaaatct tagaacggct
      301 taaaatcaga tatgataaag ggaaatagtc ttatctaggt aactaagtgc tacaaacaca
      361 gtcgaggatc acttgttagg attacatttt aatcatgctt aataagaatg gagaattcct
      421 atggggattg tattactctg aagtttcgaa taccttagtt ctcgtgattt tgagctattc
      481 taggtttaga atccttcaga atgtaaaatt ttaagcagtc aaagacttag aatagcccga
      541 aaacatttat tttatagcaa ttctaggctt agaattgctc atatatatgt atttgagctg
      601 ctcgatagtt aggacagctt tattctgaga aatctgagct atcaatgctt cagaatagta
      661 tttcgccaat ggtttttatt gaagaggtga ttgttttatg atagtctaaa ataaaactga
      721 tttaatggag taaatggaga atcgttacat gaaacatttt tttgcaggaa ttggtgagat
      781 aaaacttgtt ggctacttat tgtatatatg tgtaggacta gcccctcttt ttcatatata
      841 tgtcataggt tcggaactga gttttgtgaa aatagtgtta tctattttgg gagttatatt
      901 tgtaagtatg ttaactattg ctcgaattta tcggactttt ctataaagaa tagtttggac
      961 ttgaactgta tctaaaaaca gaggttggtc gattatcagc ctctttcttg tcgttgaaaa
     1021 gagtgagata tattgacttt tgaaaaaaag atgtcataat tgagttggga taaacctaaa
     1081 agtaatatag tagattgaat ctggaatagt ccgttatgac tgttaaaata tttctagaaa
     1141 ttaatttgac tttcctaatc gatttgttca catcttgttt caatctacta tactttctcg
     1201 ttaaaccgtt cggcaattca tggtgacatg gacacatagt agtaagcaca ctatgtggct
     1261 tggcagagct aaaaactgtt cccttgcgat aagcctaata agcacaacat agggagttag
     1321 agaagccgac tctaatcaac cactttgggc agtagtgaga actgccccgt gcttttttat
     1381 tttgagaaaa tatggagttt gtcgttgaaa ttacttgatt gtattttgga ttatcaagaa
     1441 agattcaata gaaaagcatg tcaagtatca acgaattata agtatttaga gactttcaaa
     1501 gtgaattttt gcttgactga tttacatcat ttatttggct tacacaaaat cacacgagat
     1561 tatgctagtc aaacaaaacc tgctattcaa gctggtgttt ttattttaga agattttaga
     1621 aatatcctat gtacaatgat gtaatcgaaa ggatatcttt atatgagttt atcggcgata
     1681 tcttctattc taagctaaca agttgttgca tcgtagctaa ggatttatct aaaaatacta
     1741 tgaaattgga cgtcatattt tttgaggata aaaataaaag atccgcagtt ttaggtttac
     1801 gaagagacaa aagcggagta tttaaatcag ttactctaca ttttacaagc gctaagaaat
     1861 atgctaaagt tcgtaaaaca gatgtgaaag aaatgaaatg gctataaaga ttgtccgcag
     1921 gcttaagctt gcgttttttg ttgtttaacg aaattaaaaa ataagctctc tcatcggaaa
     1981 tgtttttata ggaatgaagg aagatgatgc tatgtttaaa aagagaatcg aaaagggcaa
     2041 aagtagtgtc tttatctttt tggagtagaa gcttatgata tagaaatttg taataaaaat
     2101 gtaatataaa acatttgaca aatatttacg gggggagtat acttaatata agtatagtct
     2161 aaaaatgact atcagaaaag aggtaaattt agatgaataa gaaaaaaatg attttaacaa
     2221 gtctagccag cgtcgctatc ttaggggctg gttttgttac gtcttcgcct actgttgtaa
     2281 gagcagaaga agctccacaa gttgtcgaaa aatcttcatt agagaagaaa tatgaggaag
     2341 caaaagcaaa atatgacgct gctaagaagg attacgacga agctaaaaag aaagcagcag
     2401 aagctcagaa gaaatatgat gaggatcaga agaaaactga ggataaggca aaagcggtta
     2461 agaaagttga tgaagaactt caaaaagcaa atttagaact tcaaaaagct catgtaaaat
     2521 atcaaaaagc acaaagagaa cttagtgaaa gtccagacag aagaaaatct gctgctagaa
     2581 agaaattagc tgatgcatta tcttatattg gagaggtaga gttaaaacaa aaagaggcgg
     2641 aagcgaattt taatacggaa caagcaaaag taattcccaa agaaactgag ttagctgtta
     2701 ctaaacaaaa agcggaagaa gctaaaaaag aagcagaagt agctaagaaa aaatttgata
     2761 aggcagctca agaggtagaa gtagcgaaga aagaagtaga ggctaaggaa cttgaaattg
     2821 aaaaacttca agatgaaatt tctactttgg aacaagaagt tgctactgct caacatcaag
     2881 tagacaattt gaaaaaactt cttgctggtg tggatcctga tgatacagaa gctatagaag
     2941 ctaaattaaa aaaaggagaa gctgagctaa acgctaaaca agctgagtta gcaaaaaaac
     3001 aaacagaact tgaaaaactt cttgacagcc ttgatcctga aggtaagact caggatgaat
     3061 tagataaaga agctgctgaa gctgagttgg ataaaaaagt tgaatctctt caaaacaaag
     3121 ttgctgattt agaaaaagaa attagtaacc ttgaaatatt acttggaggg gctgattctg
     3181 aagatgatac tgctgctctt caaaataaat tagctactaa aaaagctgaa ttggaaaaaa
     3241 ctcaaaaaga attagatgca gctcttaatg agttaggccc tgatggagat gaagaagaaa
     3301 ctccagcgcc ggctcctcaa ccagagcaac cagctcctgc accaaaacca gagcaaccag
     3361 ctccagctcc aaaaccagag caaccagctc cagctccaaa accagagcaa ccagctccag
     3421 ctccaaaacc agagcaacca gctaagccgg agaaaccagc tgaagagcct actcaaccag
     3481 aaaaaccagc cactccaaaa acaggctgga aacaagaaaa cggtatgtgg tatttctaca
     3541 atactgatgg ttcaatggca ataggttggc tccaaaacaa cggttcatgg tactacctaa
     3601 acgctaacgg cgctatggca acaggttggg tgaaagatgg agatacctgg tactatcttg
     3661 aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt atcagataaa tggtactatg
     3721 tcaacagcaa tggcgctatg gcgacaggct ggctccaata caatggctca tggtactatg
     3781 tcaacagcaa tggtgatatg gcgacaggct ggctccaata caacggttca tggtattacc
     3841 tcaacgctaa tggtgatatg gcgacaggat ggctccaata caacggttca tggtattacc
     3901 tcaacgctaa tggtgatatg gcgacaggat gggctaaagt caacggttca tggtactacc
     3961 taaacgctaa cggtgctatg gctacaggtt gggctaaagt caacggttca tggtactacc
     4021 taaacgctaa cggttcaatg gcaacaggtt gggtgaaaga tggagatacc tggtactatc
     4081 ttgaagcatc aggtgctatg aaagcaagcc aatggttcaa agtatcagat aaatggtact
     4141 atgtcaatgg cttaggtgcc cttgcagtca acacaactgt agatggctat aaagtcaatg
     4201 ccaatggtga atgggtttaa gccgattaaa ttaaatcatg ttaagaacat ttgacatttt
     4261 aattttgaaa caaagataag gttcgattga atagatttat gttcgtattc tttaggtacc
     4321 tatcttatga tttcaggaaa tgtcattaaa aaaacgactc cttttctcta acttgaaaaa
     4381 tagattagag aaaatgggtt gttttattta ttatagttat ttgaatgaag ataagaagaa
     4441 ggtatactca catcattcac ataatctgta tattgactat aagttttaaa aaacaatttt
     4501 taagctcttc cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct
     4561 tcaatcgtga agcacacacg accttcaatc gtgaataaac gaatagatgg agacttacca
     4621 tgagtgataa ctctaaaaca cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg
     4681 tgacggctct tttgctcaag gagcagggct acgatgtgat cggtatcttc atgaagaact
     4741 gggatgacac agatgaaaac ggcgtttgta cggcgaccga agattacaag gatgtggttg
     4801 cggtggcaga ccagattggc attccctact actctgtcaa ttttgaaaaa gagtactggg
     4861 accgcgtttt tgagtatttc ctagcggaat accgtgcagg gcgcacgcca aatccggacg
     4921 ttatgtgcaa caaggaaatc aagttcaagg cctttttgga ctatgccatg accttggggg
     4981 cagactatgt agcgactggg cattatgctc gagtggcgcg tgatgaggac ggcactgttc
     5041 acatgcttcg tggcgtggac aatggcaagg accagaccta tttcctcagc caactttcgc
     5101 aagaacaact ccaaaaaacc atgttccctt tgggacattt ggaaaagcct gaagtacgca
     5161 gactagcaga agaagcaggc ctggcgactg ctaagaagaa agattcgaca gggatttgtt
     5221 ttatcggaga aaagaacttt aaaaacttcc tcagcaacta cctgccagct cagcctggtc
     5281 gcatgatgac tgtggatggt cgcgatatgg gcgagcatgc aggtcttatg tactatacaa
     5341 tcggtcagcg tggcggactc ggtatcggtg ggcaacacgg cggtgacaat gccccttggt
     5401 tcgttgtcgg aaaagatcta agcaagaata ttctctatgt aggacaagga ttctaccatg
     5461 attcgctcat gtcaactagc ctagaagcca gtcaagtcca ctttactcgt gaaatgccag
     5521 aagagtttac gctagaatgt acggctaaat tccgttaccg tcagcctgac tctaaggtga
     5581 ccgtccatgt caaaggagac aaggcagaag ttatctttgc agaaccacaa cgcgcgatta
     5641 caccaggaca ggcagttgtc ttttacgatg gcgaagagtg tctaggtggc ggtttgattg
     5701 acaatgctta ccgcgatgga caagtttgtc agtacattta gattgacaaa ttttctcaat
     5761 ttgctacaat aataacagca atagaaatga tggtcaaagt tcatggatgt tgcaggcttt
     5821 tttgtcctgc acttctttgt agttttgact gtttttgtgt cgtttaaggg aaaggacaag
     5881 aatgactcaa caagactttc ggacaaaagt agacaatacg gtttttgatg ttcgggcgac
     5941 agctctgatt cttcaaaatc gcaagcttct agtcaccaaa gacaagggca agtattacac
     6001 tatcggcggt gcgattcaag tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa
     6061 ggaagaactg ggtgtcaaag ctcaagctgg gcagctagct tttgtggttg aaaatcgttt
     6121 tgaacaggac ggtgtttcct atcacaatat cgagtttcat tatctggtgg acttacttga
     6181 ggatgcccca ttgaccatgc aggaagatga gaaaaggcag ccctgtgagt ggattgactt
     6241 ggataagctc caggatatcc agctagttcc agccttttta aaaacggccc taccagattg
     6301 ggaaggccaa ctaagacaca ttcatcttga ggaataggag agaaacatga cttatcattt
     6361 tactgaagaa tacgatatta ttgtaattgg tgcgggacac gctggggttg aggcttcctt
     6421 ggccgctagc cgtatgggct gtaaggtcct gcttgcgacc atcaatattg aaatgctggc
     6481 tttcatgcct tgtaatccct ctatcggtgg ttctgccaag gggattgtcg tgcgtgaagt
     6541 cgatgccctc ggtggcgaga tggccaaaac cattgacaag acttacatcc agatgaagat
     6601 gctaaacaca gggaaggggc cagctgtccg tgcccttcgt gcgcaggctg acaaggaact
     6661 ttactctaag gagatgcgca agacggttga aaaccaagaa aatctgaccc ttcgtcaaac
     6721 catgattgat gagattttgg tggaagatgg caaggttgtc ggtgtgcgta cagccaccca
     6781 tcaagaatat gctgctaagg ctgttattgt gacgacaggg actgctctcc gtggggaaat
     6841 tatcatcgga gacctcaagt actcatcagg tcctaaccac agcttggctt ctattaacct
     6901 agctgacaat ctcaaggaac tgggcctcga aatcggtcgt ttcaagacag gaacccctcc
     6961 acgtgtcaag gcttcttcta tcaattacga tgtgacagaa atccaaccag gagatgaagc
     7021 gcctaatcac ttctcataca cttcacgtga tgaggattat gtcaaggacc aagtgccatg
     7081 ctggttgacc tacaccaatg gtaccagtca tgaaattatc caaaacaacc tccaccgtgc
     7141 gcctatgttt acaggtgtgg tcaagggagt ggggcctcgt tactgtccgt cgattgaaga
     7201 caagattgtg cgctttgcgg acaaggaacg ccaccaactc ttccttgagc cagaagggcg
     7261 caatactgag gaagtctatg tgcaaggact ttcaaccagt ctgcctgagg atgtccagcg
     7321 tgacttggtt cattccatca aaggtttgga aaatgcagag atgatgcgga caggttatgc
     7381 tattgagtat gatatggtct tacctcatca gttgcgtgca actttggaaa ccaagaaaat
     7441 ctcaggtctc ttcactgctg gtcagacaaa tggaacgtca ggttacgaag aggcagcagg
     7501 ccaagggatt atcgcgggta tcaatgcggc tctgaaaatc caaggcaagc ctgagttgat
     7561 tttgaagcgc agtgatggtt atataggggt gatgattgat gacttggtga ccaagggaac
     7621 cattgaaccc taccgtctct tgactagtcg tgctgaatac cgtctcattc ttcgtcatga
     7681 caatgctgat atgcgcttga ctgagatggg acgcgagatt ggccttgtgg acgatgaacg
     7741 ctgggctcgt tttgaaatca agaaaaatca atttgataat gagatgaaac gcctagacag
     7801 tatcaaactc aagccagtca aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc
     7861 gttgacagat gcggtgacag ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga
     7921 tgtggtggcc ttcatcggac cagctgcaga agacttggat gacaagatta tcgaattgat
     7981 tgaaacagaa atcaagtacg aaggttacat ctcaaaagcc atggatcagg ttgctaagat
     8041 gaagcgcatg gaagaaaaac gcattccagc taatattgac tgggatgaca tcgattctat
     8101 tgcgacggaa gctcgtcaga agttcaaact catcaatcca gaaaccatcg gccaagccag
     8161 ccgtatttcg ggagtaaacc cagcagatat ttctattttg atggtgtatc tggaaggtaa
     8221 aaatcgtagt atttctaaaa ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg
     8281 aatgagttca aagcttggct ttgattcatc tccaacctcc catagttccc cgaactatgg
     8341 gagctaactc aagttatgca ttttcctagc cttctggtct gattttcatt gagttttaat
     8401 ctcaaaagaa agcatagaga aaaacagctc cgaagacggg gctgttttgt tgacttgtac
     8461 tcaatgaaaa tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactgcttt
     8521 gaggttgtgg atagaactga cgaagtcagt aaccatatat acggcaaggc gaagctgacg
     8581 tggtttgaag agtattattc ttcatctggt gtgaggatca tcgggatgat gatcggttca
     8641 cgttcggtat tttcatagag gaaggggcga atagcgttga caatggcacc attgacagat
     8701 tgcacgctag catccttatt tttcagtgcg atacgaatgg cattgaagag gatacgctgg
     8761 ctttggcgaa tcaagtcgcc agactctctc atgtagacaa agcctcggct gaggatgtct
     8821 ggaccagata gaatcatctg cgatttgaag tcaacagttg caactgccag aacgacaccg
     8881 tcttcagata gatcgcgacg atctttgagg acagctgcgc caatttcacc gatacgattt
     8941 ccatcgacat agatatcttg ggcgttgaaa tgacctgcga tacgagctga gtcagcagta
     9001 agggcaagca catcgccatt gctcatgata aagatattgt ccttctcaac accagtatcc
     9061 actgctagtc cagcgtggac tttttgcatg cggtattcac cgtggacagg catgaagtat
     9121 tttggcttaa tcaagcagag catgagtttt tgctcttgct gaccaccgtg tccagatgta
     9181 tggatattgt tcactttacc gtggataact tcgacaccag cttcagaaat gatgttaatc
     9241 agcttgttga cgctagtagt gtttccaggg atgggactag aagagaagat aacggtatca
     9301 cctggttgta attgtacttg acggtgggtt ccgttggcga tacgagagag ggctgccata
     9361 ggctcaccct gactacctgt acagaggata agaacttctc ctgcaggata atctttgatt
     9421 tcatttggct cgataacggt tcccttagga gctttgatgt agccaagatc gattccgttg
     9481 acaatggcct tttccataga acgaccaaag accgcaatct tgcgtccagt cttaacagca
     9541 gcttctgttg cctgctggag acggaagata tttgaggcaa aggatgcaaa gatgatacgt
     9601 ccttcaatac cttggataat cttcataatg gactgaccaa cgactttttc agagttggta
     9661 aaggttggta cttccgcatt tgtcgagtca gacaggagac agagcacgcc ttcttcacca
     9721 agcgcagcca tacgatgcaa gtccgcaggt tctccaactg gagtaaagtc gaacttaaag
     9781 tcacccgtac agacgatttt cccttgagga gtatgaatga caatccccaa aggctctgga
     9841 atagagtgag tcgttctaaa gaaagttgcc ttgagatttt taaaggtcaa ctcggtgttg
     9901 tggttgattt cgtaaagttt ggcgttgcgc aagaggccgt gttcttcgag tttcccacgg
     9961 atcaaagcca aggcaagcgg tccagcgtag atagggacat ttgcttgctt gagtaggaac
    10021 ggaatcccac caatgtggtc ctcgtgtccg tgtgtgatta aaacagcctt gacgcggtcg
    10081 atattgtcca cgatgtaaga gtagtcagga atgacatagt cgataccaag caagtcatct
    10141 tctgggaatt taatcccagc atcgacgatg ataatctcgt cttggtattc aattccgtaa
    10201 gtgtttttcc cgatttctcc tagaccaccg atggcaaaaa cgccgacttc ttcaggttta
    10261 agagtgtagg ccatattaga actccgtaat ttcgaaggcg ccagtttctt tttcgtaatc
    10321 gagcaatttg tcagacaaga gttcgatata ttcgatattg tactctgggc gattttcttc
    10381 gacaagttgg cgagcagtga tacggccctc aagttctgag ctggcatcga tgtctaggta
    10441 aagcgcgcgt gttgtttcac ggcgtgggct acgttctttt gtttcttgat aaaaaacttt
    10501 gtaaatcata tagttccttt ctatttacag gtcagcttat tccaaacttt tagcagatat
    10561 ttgcttgatt tcattccatt ttgtgtctag attgaccttg attcgttaca attatttcaa
    10621 ttataccaca aaatgaaaaa ttagtaaaag acggaaacga gaaagttagg ggacgatttc
    10681 tttggtgagc gtttgcaaaa ataaagaaaa tgtattatgt tgataacgga agtgaaattt
    10741 attgcttgtc ataattccat atagatttta agtcaaggag gttgccctat gtataactta
    10801 tttgcttttg ctatcggagt tcgtttgtta gcttttatcg tttttgttgc ttgcgtttat
    10861 ggaggaaatc ttctatttgc caaactggaa cgatgacaaa ccaagttctt gaggaaaatt
    10921 acctttgtga ctctctactc gctttttctc ctcctcgtga cctatgtcgt ttggtttgta
    10981 ttttactttg gattaaatgc ttagatgccc tgtttatgct ggggtttttt gtttgggaat
    11041 gtagagatag tagatttacg acacgaaaat tccaccccat ctatcaagat gaggtggtct
    11101 ttgcattttt ctaacatgac atgacaatgg aattttcaaa gcttattatt tttatgggag
    11161 tcattgttct gctcaaaaat agagttgaag cataaaaata aaacaataca aacagataaa
    11221 tcgaatggta aagggatagt agtaatgttc aaatcattga gaatgctgag caaactgtaa
    11281 gagatgaaga caataaaatt tatccttcta aaaatgtgtt tcttatctag catgacttac
    11341 caaacttttt acgaaggtct tttccaatgg tataacctgc cataaataaa gcgataccaa
    11401 cacctacacc aaagccaccc ttgatttctt gcaattcctt attagaaatt tcacgatagt
    11461 tgttcaaatt tttcatcata ttaatctcct cctaaaaaat aaccagctgc caaaccagaa
    11521 cctataagtg ctgttccacc tgcaatagct ttccaagata ctgctactcc aaagataacc
    11581 aagggagcga gaccgccatc aatatctgat aattcatttt cagataagga taaaaattga
    11641 ttgtcaagaa tgtcaaaatt tgtcattgaa ctttcctcct tttttcttgt tcacttttat
    11701 attcggaaaa gaaaaggaaa agtgagcatt tttttaaatt atttttagtt aagtgagggc
    11761 actcttgtct gggggcaaag aaaattccca tgtgccagct tttgtagtat aatagtaagc
    11821 agacaaaaag ataggaatgt gttatgaaag tattagcttt tgatacgtcc agcaaggctc
    11881 tttctctggc tattttagag gataagcagg ttcttgccga gacgacgatt aatattaaga
    11941 aaaatcacag tattactctt atgcctgcca tcgatttttt gatggcaagt ttggattgga
    12001 cacccaagga tttggaccga atcgtggtag ctgaagggcc gggtagctat acaggcttgc
    12061 gaattgcggt agcaactgct aagaccttag cttacaccct gaacatcgag ttggttggta
    12121 tgtcgagtct cttggctctg gtgccccacc aacaagaagg tttgtttgtc cccttgatgg
    12181 atgcgcgtcg caataatgtt tatgcaggat tttatgaaaa tgctaaaccc gtcatgccag
    12241 aagctcactt accctttgag cgagtgattg agttaatcaa gggtgctagt caggttacct
    12301 ttgtcggaga agttggcccc tttgttgagc agattcaaga atacttgcca aggactaatt
    12361 tcaaagaaac cttgccaaat gcagctaatc tagctctatg ggcctgggac aaggaagcag
    12421 actccttgca tgattttgtg ccgaattacc tcaaacgagt cgaggctgag gaaaactggc
    12481 tcaagaacca taccgagtct ggcgagtctt acattaaacg cctatgatag aaatcaagcg
    12541 aattcaacaa cagcctgacc tagctcaagc catctacgct gttatggcag ctgtttacct
    12601 agtcagtcct tggactctgg agcaaatcca agcagatctg tcccaagacc agacttggta
    12661 tgcattggct tatgatgggg cagaagtgat tggatttcta gctgtgcagg agaatctttt
    12721 tgaagcagaa gtcctgcaaa tcgctgtcaa aggagcttat cagggtcagg gaattgcgtc
    12781 agccttgttt gctcaattgc cgacagacaa ggaaattttc ctcgaagtca gacagtcaaa
    12841 tcaacgagcg caagcatttt acaagaaaga aaagatggca gttatcgctg agcgaaaggc
    12901 ctactaccat gacccagtcg aggacgccat tatcatgaag agagaaatag atgaaggata
    12961 gatatatttt agcatttgag acatcctgtg atgagaccag tgtcgccgtc ttgaaaaacg
    13021 acgatgagct cttgtccaat gtcattgcta gtcaaattga gagtcacaaa cgttttggtg
    13081 gcgtagtgcc cgaagtagcc agtcgtcacc atgtcgaggt cattacagcc tgtatcgagg
    13141 aggcattggc agaagcaggg attaccgaag aggacgtgac agctgttgcg gttacctacg
    13201 gaccaggctt ggtcggagcc ttgctagttg gtttgtcagc tgccaaggcc tttgcttggg
    13261 ctcacggact tccactgatt cctgttaatc acatggctgg gcacctcatg gcagctcaga
    13321 gtgtggagcc tttggagttt cccttgctag cccttttagt cagtggtggg cacacagagt
    13381 tggtctatgt ttctgaggct ggcgattaca agattgttgg ggagacacga gacgatgcag
    13441 ttggggaggc ttatgacaag gtcggtcgtg tcatgggctt gacctatcct gcaggtcgtg
    13501 agattgacga gctggctcat caggggcagg atatttatga tttcccccgt gccatgatta
    13561 aggaagataa tctggagttc tccttctcag gtttgaaatc tgcctttatc aatcttcatc
    13621 acaatgccga gcaaaaggga gaaagcctgt ctacagaaga tttgtgtgct tccttccaag
    13681 cagcagttat ggacattctc atggcaaaaa ccaagaaggc tttggagaaa tatcctgtta
    13741 aaaccctagt tgtggcaggt ggtgtggcag ccaataaagg tctcagagaa cgcctagcag
    13801 ccgaaatcac agatgtcaag gttatcatcc cccctctgcg actctgcgga gacaatgcag
    13861 gtatgattgc ctatgccagc gtcagcgagt ggaacaaaga aaactttgca aacttggacc
    13921 tcaatgccaa accaagtctt gcctttgata ccatggaata aagagtggct ctttgtcaag
    13981 tgtagtgggt aggcgaaaag ctacaatctg gagagtacga aattcgtact ctcttttttg
    14041 atgttcaaag tcatcaaaat ccgtttttta aaattgttaa agttccggaa accaaaggct
    14101 ttgcgcttga taaccttaat caaattattg gtcgcttcaa gtttagcgtt ggaataaggc
    14161 agcttaagtg cgttgataat cttatctcta tcccttaaaa aagtccaaaa gaccgtttga
    14221 aaaagcggat gaaccgttgg cagttcttgc tcaatcaatc caaagaaatg gtcggcattc
    14281 ttctcttgaa aatgaaagag caggagttga tagagttcgt agtaaacctg taatccctca
    14341 gagtaagaaa gcagcttatc tcgtacttcc cgatgggtta agtgcattcg aaacgtgggc
    14401 tgatagaatc gtttctgaca gagcttgaag ctatcctggt gtagtaaatc agtccagtgg
    14461 actgatttag cccgagccta gaaacgaaaa agcgagggtt ccaaaagcgc ttgagcgccc
    14521 gatactctaa ggattttcgg tcaaattggt tcatgatttg gattcgtact cggttcatag
    14581 ctcggctcag atgttggaca atgtggaagc ggtcaagaac aatcttcgcc tttggaaaga
    14641 gttgcttagc gagccgataa taagggctgt acatgtccat ggtgatgacc tcgaccagct
    14701 cccgaacctc tctctgatag cgttggaaat ggtttcgaat caccgcataa gttcgcccgt
    14761 ctaaaattgc gaggatggat ttggactcaa aatcttgggc aatgaagctc attttgctct
    14821 ttttgaagct atactcatcc caactcataa cttttggcaa cttggtccaa tcggtttcaa
    14881 acttaaattc cctcagtttt cggatgacgg tggaagttga gaccgccaat ctgtgggcaa
    14941 tctcagtcat agactgcttt tcaaggagga gctgagcgat tttttgtctc accatgttgg
    15001 aaatctggca atttttcttg acaatggatg tttgagaaac gaccacctta aggcaattct
    15061 tgcactgaaa gcggcgcttt ttgagatgta gtaccgtggg taaaccctga cagtcgagaa
    15121 gcggaatttt agaggctttc tggaagtcgt atttgatcat cttcccttga caatgaggac
    15181 aaggaggagc gggggaatcc aactttgcct gaacgactag atgggtttgg tatttcagaa
    15241 cagacaagat tttgatattt ttgtctttca atccgagcaa atctgtggta ttcttaagtt
    15301 gttccatatg aaccctttct aatgagttgg ttagacgctt ttcattatag gtcatatggg
    15361 gctttttttc tacaagaaac gaccctataa ttcctggggt gggattaccc actacagaaa
    15421 ttatagagcc aaagcattcc aaagtcttgt ctgactctgg aatgttttct gttcacagtt
    15481 gttttgtaac tgcctaataa gtaaccgcgc tataacgagg tatctaccga gattgtcaat
    15541 tctccagtat actgttataa aaaacaaaac tggaggattt ttttatgtcc ccccatcgtc
    15601 ccatcgactg ttccgtagct gtccaataac ggagtataat cagctgaaac aagaatagga
    15661 caaaagtgcc tcaagaaaag catcgtaatt tgacaatatt attctgagat gtttttgatg
    15721 taagcccata ttttctcaat gggattatac tcgggtgaat aggtaggaag tggtaacagt
    15781 ctatgccctg ctccttacac atgttcattc tgtgaaacct tgcattgtcc ataatgataa
    15841 gggatggtgt atctaaagta ggtagtaaga atttttggaa gcaagcttcg aaaaagtcac
    15901 tcgtcatcgt atctttatag gtcatcggag ccatagtata ttgaaacaaa aaagggcatt
    15961 agtacttcta taacagtatc gcaatctctg gtgtgcgttg tctcgatctt aattcaaata
    16021 attatatctt cttccagaga gactaactgc ctgctcaagg ctcgatcata ttcgagataa
    16081 aaataggtct caacccctgt ctcataaata taaatataaa taggagtcag gtagcttaag
    16141 tgattcaatt ctttaaggaa ccgatttact ttttctgggt cttgttcgca gtaggtacgg
    16201 ctctttttta gactatatcc catagccttt agagcgtaat gaatagttgt tggaggacag
    16261 ttgaattcag cagctatttc agtcaaataa gcatcgggat gagtttcaat atagatcttt
    16321 aatttatctc tatcaatttt ttcaattaat agtggaagtt ttcttgaagt tagtgtattg
    16381 aaacgttttt atacagtgct aaaatttgaa gtgtagtgca gatgtatttg taatagtatt
    16441 atagcaattc tttggagaag gtcgagacaa gag
//
LOCUS       DAVGJJ010000040        16011 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000040 DAVGJJ010000000
VERSION     DAVGJJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.040"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            103..561
                     /locus_tag="VVE35_001191"
     CDS             103..561
                     /locus_tag="VVE35_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU3061385.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            574..1182
                     /locus_tag="VVE35_001192"
     CDS             574..1182
                     /locus_tag="VVE35_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061386.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRFENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1253..1585
                     /locus_tag="VVE35_001193"
     CDS             1253..1585
                     /locus_tag="VVE35_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061387.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1596..3005
                     /gene="ply"
                     /locus_tag="VVE35_001194"
     CDS             1596..3005
                     /gene="ply"
                     /locus_tag="VVE35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU3061388.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
     gene            3888..4604
                     /locus_tag="VVE35_001195"
     CDS             3888..4604
                     /locus_tag="VVE35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3061389.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4728..5197
                     /locus_tag="VVE35_001196"
                     /pseudo
     CDS             4728..5197
                     /locus_tag="VVE35_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5214..6035
                     /locus_tag="VVE35_001197"
     CDS             5214..6035
                     /locus_tag="VVE35_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061390.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQSA
                     SMMMGMPFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLM
                     RTTNDITQVQMLITMDLQVVTCGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPVMMGISSGLSVAIYWIGAYVINDVAPIARLPLFSDMIVFMSYAM"
     gene            6042..7073
                     /locus_tag="VVE35_001198"
     CDS             6042..7073
                     /locus_tag="VVE35_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3061391.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPAQVQLADE
                     NFKGQVEFKDVTFRYAANLEAVIEHVSFKVETGQTVAFIGSIGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARSLLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            7139..7501
                     /locus_tag="VVE35_001199"
     CDS             7139..7501
                     /locus_tag="VVE35_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU3061392.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7498..8001
                     /locus_tag="VVE35_001200"
     CDS             7498..8001
                     /locus_tag="VVE35_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU3061393.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYEAWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8115..8561
                     /locus_tag="VVE35_001201"
     CDS             8115..8561
                     /locus_tag="VVE35_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061394.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8566..9240
                     /locus_tag="VVE35_001202"
     CDS             8566..9240
                     /locus_tag="VVE35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061395.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            9401..9592
                     /locus_tag="VVE35_001203"
     CDS             9401..9592
                     /locus_tag="VVE35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU3061396.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            9877..10176
                     /locus_tag="VVE35_001204"
     CDS             9877..10176
                     /locus_tag="VVE35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU3061397.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10173..10490
                     /locus_tag="VVE35_001205"
     CDS             10173..10490
                     /locus_tag="VVE35_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU3061398.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            10506..11132
                     /locus_tag="VVE35_001206"
     CDS             10506..11132
                     /locus_tag="VVE35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU3061399.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            11174..11935
                     /locus_tag="VVE35_001207"
     CDS             11174..11935
                     /locus_tag="VVE35_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU3061400.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12013..12408
                     /locus_tag="VVE35_001208"
     CDS             12013..12408
                     /locus_tag="VVE35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU3061401.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12678..12962
                     /gene="groES"
                     /locus_tag="VVE35_001209"
     CDS             12678..12962
                     /gene="groES"
                     /locus_tag="VVE35_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU3061402.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            12978..14600
                     /gene="groL"
                     /locus_tag="VVE35_001210"
     CDS             12978..14600
                     /gene="groL"
                     /locus_tag="VVE35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU3061403.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            14738..15493
                     /locus_tag="VVE35_001211"
                     /pseudo
     CDS             14738..15493
                     /locus_tag="VVE35_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4818 a         2891 c         3658 g         4644 t
ORIGIN      
        1 tttcgcagga agtctaataa gttatagtcc agtttttact atactttgat attcaaatat
       61 gttatactat gattatcaaa gtaaatgaaa ggagttacga atatgacgcc agaagaaatg
      121 tacctgacag agcgattaga cgtacagata gctcattttt taaagaaaag cgttcaacat
      181 cgtaggcgct ataaggtatt aaaaataaca gaaatcgtgg caggtttcct catagctgtc
      241 ttttgtgcta ttcctatgcc aggtgatcgc taccgtttga tttcggttgc cttatccagt
      301 ctcggcttgc tgtgtgaggg gattatcaat ttgtataatg caaaggaaaa ttggatttct
      361 taccaaaaaa ctgcgcaact cctggagaaa gaaaaattcc tctatcaatg ccaaacggag
      421 aaatatgcag gaaagaccaa ggcttttgcc ctatttgtca agacatgcga aggtcttatc
      481 tcagaggaga ttaaccagtg ggaaagtatc cagtcaaaag aagtggcagc tagtgcagat
      541 gctccagtta aaaaagagta ggaggtagag gaaatgtctc aatccagcta cctgtcgccc
      601 ttgctctggt taaaaaaaga agccgataag gaaaagatga gcgcgaccca gtgccagata
      661 tttttctttt actatcaaat gtttgagctc ttatttgcta gagaaagcga catgaaagac
      721 ttatgtctgg gaacgaaagg tttttatttc tcgcagttag agaaaaattt gctttctgga
      781 gtttcccgat ttctaaaaaa cttggagggg aaagtaactc tcaaggctaa ccaagaagta
      841 tcagctcgca aagccctttt tctagccttg acaactagcc aatcagattg gcaggagtta
      901 gctcctgttt ttgattttta tcagactatc gggaggtttg aaaatccttc tctcttgagt
      961 tctcaggaca gacaacatct gatgtggatt taccagtcag ctttggagaa ggattatatt
     1021 gtcaaggtta ttggcgacaa gcattttgta ttgaagagac aagatgctac taaattgaca
     1081 gcgcgccaaa ctcaaacttt ggaaattctg agtcaatcag aagacttggt caatcctgtc
     1141 tatgttacat taggagaaaa gggggtgctc ttgcttgatt aagagaggag atgttgtagt
     1201 tctttatttg ccttttccga ctattagtag cgatttggct gtgaagaatc atatgtatat
     1261 ctgtattgac aacagcatga ctaaaaacaa agagttggtt aaaaatcaga ccttcaaacc
     1321 agctctcttg accagacgtt tggtcaagaa ctttatgata gaagagccgg atctagctcg
     1381 taatcctttt acaagaccaa ccttgattga cttagataag gtatttatgt tggataatac
     1441 ggttattccg acttcttatc tagccagacg gcgacgcaat gtctcagaag aattgtacga
     1501 ggaaattttg gatcacttag tccaaccacg gctgatttcg ctgaacaagt ctgagtttat
     1561 gcaactcaat ccaggaactt attaggaggt agaagatggc aaataaagca gtaaatgact
     1621 ttatactagc tatgaattac gataaaaaga aactcttgac ccatcaggga gaaagtattg
     1681 aaaatcgttt catcaaagag ggtaatcagc tacccgatga gtttgttgtt atcgaaagaa
     1741 agaagcggag cttgtcgaca aatacaagtg atatttctgt aacagctacc aacgacagtc
     1801 gcctctatcc tggagcactt ctcgtagtgg atgagacctt gttagagaat aatcccactc
     1861 ttcttgcggt cgatcgtgct ccgatgactt atagtattga tttgcctggt ttggcaagta
     1921 gcgatagctt tctccaagtg gaagacccca gcaattcaag tgttcgcgga gcggtaaacg
     1981 atttgttggc taagtggcat caagattatg gtcaggtcaa taatgtccca gctagaatgc
     2041 agtatgaaaa aatcacggct cacagcatgg aacaactcaa ggtcaagttt ggttctgact
     2101 ttgaaaagat agggaattct cttgatattg attttaactc tgtccattca ggagaaaagc
     2161 agattcagat tgttaatttt aagcagattt attatacagt cagcgtagat gctgttaaaa
     2221 atccaggaga tgtgtttcaa gatactgtaa cggtagagga tttaaggcag agaggaattt
     2281 ctgcagagcg tcctttggtc tatatttcga gtgttgctta tgggcgccaa gtctatctca
     2341 agttggaaac cacgagtaag agtgatgaag tagaggctgc ttttgaatct ttgataaaag
     2401 gagtagctcc tcagacagag tggaagcaga ttttggacaa tacagaagtg aaggcggtta
     2461 ttttaggggg cgacccaagt tcgggtgccc gagttgtaac aggcaaggtg gatatggtag
     2521 aggacttgat tcaagaaggc agtcgcttta cagccgatca tccaggcttg ccgatttcct
     2581 atacaacttc ttttttacgt gacaatgtag ttgcgacctt tcaaaacagt acagactatg
     2641 ttgagactaa ggttacagct tacagaaacg gagatttact gctggatcat agtggtgcct
     2701 atgttgctca atattatatt acttgggatg aattatccta tgatcatcaa ggcaaggaag
     2761 tcttgactcc taaggcttgg gacagaaatg ggcaggattt aacggctcac tttaccacta
     2821 gtattccttt aaaagggaat gttcgtaatc tctctgtcaa aattagagag tgtaccgggc
     2881 ttgcctggga atggtggcgt acggtttatg aaaaaaccga tttgccacta gtgcgtaagc
     2941 ggacgatttc tatttgggga acaactctct atcctcaggt agaagataag gtagaaaatg
     3001 actaggagag gagaatgctt gcgacaaaaa gaggcgatga tctctctgcg gatatctgaa
     3061 agtttatctc ttgcctagcg atttccattg gagtatgctc gtgatgttaa aatgaatcta
     3121 tcaaacagaa tgtgtaaaaa agtaaaaagg gtagccaatt ccttgatata caggggattg
     3181 attatccttt acttgtttta gatgattgga aattcaagaa tgacacgtat cggaaatatt
     3241 ttgctgttta tatctaatac ccatttatgg aaaaaatgaa attaaatatg aacaaatcaa
     3301 ttgctagcaa tggtttagaa gtgccagtgt actagtctaa cttctccatc ttgggaacac
     3361 gtaaattata ctcttcgaaa atctcttcaa accatgtcag ccctatccgc aacctcaaaa
     3421 cagtgttttg agcaacctga ggctagcttt ctagtttgct ctttgatttt tattgagtat
     3481 tagagatttg cttagaaact cttctattga tttcttagaa tacaatagcg cctataagaa
     3541 ctgttggatt tcaaagtatt tacttgaact atgatatgtc tggagtagac tgtcaacaaa
     3601 aggtgcagta tgattgtttt tgtcgcttat atctacaaac ttcaaacagt aatttgagct
     3661 gactttgtca aacttggctg aaactttcta tttgcagata atggtctagt ttgttttttg
     3721 aatgtggatg agtgtctgtt gataaaaaga tagaaaggca agaccatctt agcaggtaaa
     3781 gtgctatcct agcttgattt gctaataaaa tccatttcct ctagtgaaaa tcaaaaaaac
     3841 ttgtgttata ataagaaaga ttaaaatgtg aaaaaaggag attcctaatg ggacgtaaat
     3901 gggccaatat cgtagccaag aaaacggcta aagatggagc aaactctaaa gtatatgcaa
     3961 aatttggtgt agaaatctat gtagcagcta aaaaaggtga tccagatcca gaatcaaact
     4021 cagctttgaa attcgttatc gaccgtgcta aacaagccca agtgccaaaa cacattatcg
     4081 ataaagcgat tgataaagcc aaaggaaaca cagacgaaac ctttacagaa ggacgttacg
     4141 aaggttttgg gccaaatggc tctatgctaa ttgtggatac tttgacttct aacgtcaacc
     4201 gtacagcagc caatgtccgt gcagcctttg gtaaaaacgg cggaaacatg ggcgcttcag
     4261 gttctgtttc ttacctcttt gacaacaaag gtgttattgt atttggaggt gaagatgcgg
     4321 acgcagtctt tgagcaattg ctcgaagcgg atgtggatgt ggatgacgta gaagcacaag
     4381 aaggtacaat cacagtttac acagcgccaa ctgaccttca caaggctatc gttgccctcc
     4441 gtgagtctgg tattgaagaa ttccaagtga ctgaattgga aatgattcct cagtcagaag
     4501 tggaattgtc aggcgaagac cttgaaacct ttgaaaaact ttacagcgtt cttgaagacg
     4561 acgaagacgt ccaaaagatt tatacgaacg tagatgggtt ttaatttaac ctatagaaca
     4621 tgatcctaag tgagaaaatc acttgggatt tttttaatca agaaaatagt tctcatagaa
     4681 atttaattat tctccagaga actattttta tttagaaggg ggagattatg ggtaaaccga
     4741 tgttagtctt taagcgtttt ggtcatcaaa ttcacctgat ggtgcaaaag gaagccaaac
     4801 gttgcggcat tgaatttatg ggtgggccgc aaggtcaggt tgtgcgtttt ttggataatc
     4861 gcgagaaaaa ccaagacttg gtcttgatta aagatatcga gcaggaactc aatattacca
     4921 agtctgttgc tagtaatctg gttaagcgta tagtgcaaaa tggtttggtg gaattggagg
     4981 cgagtcctgt tgataagcgg gctaagtttg ttcgtctgac ggacaaagca cgttctcaga
     5041 tgcaacaggt taaggctttt ttgaacgcat agacaagcag ttgatggaag acattgatga
     5101 agatgaatta ctgatttttg agaaagttct cggtcaacta caggcaaata tcaagggaat
     5161 aggaggagag aataaagaaa ttagtcaaaa gaattagtag aaaagaatgg gggatgattt
     5221 tactagccat tctctttact tgcttttcgg tctatctaga gttggaagtg ccgacctata
     5281 tctcgaaaat tacggatttg ctaggtagtc aaggaactaa tttagatgag ttgtggcagt
     5341 cggcaagcat gatgatggga atgccctttc ttgccttctt gtccgtagtt gcagttggat
     5401 tttttgcatc ccgagtggcg gcttcttata ctagtaggct gagaagtgat atttttaacc
     5461 gagttttgga ttactcgcag acagagatta agaaattttc aattcctagc ctcttgatgc
     5521 gtactaccaa tgacattact caagttcaaa tgttgattac tatggacttg caagtggtaa
     5581 cgtgtggtcc aattatggct atctgggcta ttgggaagat tttaggtcat tcagaatact
     5641 ggctctgggc cgtacttgtg gcagtgattg tcaacgtcct gatgacgacc gttttgatga
     5701 cgctagcctt tccaaaacag tccttgattc aggggctgac agataaactg aacagtatca
     5761 ctcgtgagag tttaacaggt attcgtgtcg ttcgtgccta caatgcagag gattatcaaa
     5821 atgaaaaatt tgcagcagta aatgatgaat tgacccgttt gaatttgttt gtcaaccgtc
     5881 ttatggctat tttgaatcct gtcatgatgg ggatttcaag tggtttgagt gtagcgattt
     5941 actggattgg ggcctatgtg attaacgacg ttgctccgat agcgcgtctg cctctcttta
     6001 gtgacatgat tgttttcatg tcttatgcca tgtaggttgt catgggcttc cttctcatgg
     6061 gagcactctt catcgttctt ccccgaacta tggtctctgc taagcggatt aatcaagttt
     6121 tagatttgca ttcttctatc caaaaccctg ctcaagtgca gctggctgat gaaaacttca
     6181 aaggtcaggt cgagtttaag gatgtgacct ttcgctatgc ggcaaatttg gaggcagtta
     6241 ttgaacatgt tagctttaaa gtagaaactg gtcaaacagt ggcctttatt gggtcaatag
     6301 gttctggtaa atcaactctg gtcaatctga ttccacgttt ctacgacgtg tcagcaggag
     6361 aaattctggt ggacggtgtc aatgttcaag actatgactt ctctgcgaca gctcatgctg
     6421 gtcaaaaggt tgccattgtt gggccgactg gggctggtaa gacaaccatt gtcaatcttt
     6481 tgatgaaatt ctatgagatt gataagggaa gtattcgcat tgatggtgtg gataccaagg
     6541 ctatgacgcg ttcagaagtg catgatgcct tttcaatggt cttgcaggat acctggctct
     6601 ttgaaggaac tattcgagac aatctcatct ataatcaaat agggattagt gatgaacgaa
     6661 tgatggaagc tagtaaggct gtgggaattc accactttat tatgaccttg ccagatggct
     6721 atgataccat cttggatgac accgtgacct tgtctgtagg acaaaaacaa ttattgacta
     6781 ttgctcgttc ccttcttaag gatgcaccgc ttttgatttt ggatgaggcg acttcttctg
     6841 ttgacacacg gacagaggaa ttgatccaaa aagccatgga ccgtttgatg gaaggacgca
     6901 catcctttgt cattgcccac cgcttgtcaa ccatccgaaa tgcagacttg atcttggtca
     6961 tgaaagatgg aaatatcatc gagcaaggca actatgagga actgatggcg caaggtggct
     7021 tctacgctga cttgtacaat agtcaattta cagaagatga agcagaagaa taaatcaaaa
     7081 gaaggcttga cggccttctt tttcttattt atactagtac acatagaaag agagaagtat
     7141 gagtcagaaa ttatacaata tgaaatttgc tgctgtctac ttagccttga ttgcaaaagt
     7201 tgagcgaaag ggtggaaagg cagagtcagt tcaccaagtg accagttggt tgacagggta
     7261 tgaagtgagt gatgttcttg cttgtctgga tagggatgtt acttacggag atttttttcg
     7321 acaagcacca tattatgtcc ctgaacgaat tgcaattaca gggaagattt gcggtgtccg
     7381 tattgaggaa attgatgatc ctctgatgca ggaaatacgt cgcttggaca agctagtgga
     7441 ttggcttgcc aaggggaaaa catctcaaca agttctagaa aagtatgaga agcataaatg
     7501 aaaaattatc aagaatggta tcgaaatatc agctccagac taactagtcg tccaattctt
     7561 ttattcctat tacgcagttt caatcgtttg atgacagtcg ccatgcccct ggtctatctg
     7621 atcttgctag tcaccactta cctgcaacta ggacttggtc agcaagttgg ggtttatgtg
     7681 cttattcctg catcaggttt tgtggccttg tccctgtttc gtaagaaaat caatcacccg
     7741 agaccttatg aagcttggga tatctgtccc cttcttgata aggatagttc gggacggtcg
     7801 atgcccagtc gccatgtctt ttcggcaact atcatctcca tggcctgtct gcatgctagt
     7861 ctacctattg gcttagtatg cttgatcttt tcagctttgc tggggttggt gagggtttta
     7921 gggggtgttc attatcccaa ggatgtcttg gttggctatg cttgtggttt ggtgtgggga
     7981 ttccttttct tcctattctg acatgtaagt taagctagtc ctacaaccac ttactgcttc
     8041 aaattgacca cttgcttttt tgcccttgta ttcctaactt agctttgata tagtagagtc
     8101 agaaaggaga tgtgatgaag ttacgaattg agattgacgg caatttagag gagactgaaa
     8161 ttgtcatcaa gacccccact ttgacagatg aaattgcaga cttgcaacgg ctcttgcaag
     8221 agtcaaaggc tccgaggttg actttttaca aggggacagg tgaatattat ctagacctgt
     8281 cagaaattct cttctttgaa acagaaggga gcaagatcta cgctcataac cagaaggaag
     8341 cttatgaggt tcgcctcaag ctctatgagt tggagtctat cttgcctcgc tattttaatc
     8401 gagtttccaa gtcaacgatc gcaaacatcc gtcagattta ctcagtggac aagtcctttt
     8461 caggaacggg caccatttcc ttttatcaga cgcacaagga ggttcatgtc tcacggcatt
     8521 accaatccct cctaaaagaa aatctaagaa atatgaggta agaacatgaa aaagaaagca
     8581 tttggtattg tgttgcttgt actagcagct ttaatattag tacaaggaaa ttttggaatc
     8641 ccttctctag gagggcacat ttggcccttg attggtattg ctttttttgc ctaccaatca
     8701 gttggagctt tgttgcgtcg ccacttaact tcagcctctt ttacagctct agtagcctta
     8761 atgattgcta accactttta tggcatttta ccgattccaa accagacact aatctgggca
     8821 ggagttctaa tcgttctcgg agtaaatatg ttgactcatt ctaacagaac ttggaatggg
     8881 aaaaaatggt ggtatgatgg tgaaaaaaac attcttatag ataaggaagt tgcttttggg
     8941 acagggactt tctacaagca agaccaagaa ttggtagaag accaagtaga agttggtttt
     9001 ggcaatgcaa aaatctacta tgacaatgca gagatgttag gtgattttgc aactttaaat
     9061 attgaagtag cttttggtaa tgcaactgtc tatgttccgc aacactggcg tgtcgatttg
     9121 aaagtagaaa catcctttgg tgcagccaag gcggacgctc ctgtagcccc aaccagcaaa
     9181 accttgatta tccgtggaga tgtggctttt gggaagttgg aaattgtcta cgctaaataa
     9241 aaaaatcttc acttcaacca tcaaaatagg cgtactaaga gtaggaaatt gatgccttgc
     9301 tctgatttca gttctatggt tgttagactt taaaaaatga aatgctgcct ttaaaagttg
     9361 tatatttttc gatattttgg cttttacgtt tgatgtatct atgtactaca gcgtagatga
     9421 tgtagtgtcg aatgctttta aaaaacgaat gatattggac agtttttttg cctttaattg
     9481 ctcaggaacc atgaaagtca gtacttgggt ttatgacaag ggagaatggt attatgtaag
     9541 ttcttctggt tccatgattg cgaatgattg ggtcaaagac aatggcaagt agtattatct
     9601 ggcttcatca ggtaagatgc ttcgcaatac ctacacacct gatggttact acgttggcaa
     9661 cttaggtgcc tggcaataag aataatactc tttgaaaatc tcttcaaacc acgtcaacgt
     9721 cgccttatct acaacctcaa aaccgtgttt tgagcagcct gcggctagct ttctagtttg
     9781 ctcttagtat aagaagtcct tttccttaaa ggaagaggct ttttacttgc ttttctgctg
     9841 cttcctcatt tgtcctgaag ccttttttgt gttaaaatga atggtatgaa agctaaaaaa
     9901 atgtggatgg caggcttggc tctgctaggt atcggaagcc ttgctcttgc tacgaaaaaa
     9961 gttgcagatg accgtaagct catgaagact caggaagagt tgacagagat tgtgcgagac
    10021 catttttccg acatggggga aattgcgacc ctttatgttc aagtttacga aagcagtctg
    10081 gagagcttgg ttggtggcgt catttttgag gatggccgtc attatacctt tgtctatgaa
    10141 aatgaagacc tagtctatga ggaggaagtc ttatgataca gccagcaagt ttagaagaat
    10201 tagcatcttt agtggaaaaa gcgggcaaga aggtcttcct ttttgtggcg gactggtgtg
    10261 gcgattgccg ttatatctat cctgccttac cagaaattga ggagactaat ccagagttta
    10321 cctttattcg agtggataga gaccagtata tagatttggc caaactctgg gatgtatatg
    10381 gaatccctag ccttgttgtt ctagaaaagg acaaggaaat cggtcgtttt gtcaatcgcg
    10441 accgtaaaag caagcaacaa atcaatgact ttttagcagg actgaaatag gagaaaaagg
    10501 aaacaatgat ttttacatat aataaagaac atgtcggtga tgtccttatg gtcatcgtga
    10561 aaaatagcgg agatgccaaa ctggatgtgg agcgcaaagg caaggtagcc cgtgtttttc
    10621 tcaaagataa tggggaaaca gtggcttgga atattttcga agtttcaagt ttatttgaaa
    10681 ctgcagagcg cggtcaagtc tttttgacag atgagcaagt cgctcgtttg aaccaagagt
    10741 tgcaggcgga aggttttaca gaagaaattg ttaatgataa ggaacctaag tttgttgtcg
    10801 gtgaaattgt tgagatggta gcccatccag atagtgacca cctcaacatc tgccaagttg
    10861 cagtcgcaag tgacaagaca gtacaaatcg ttgcaggagc tcccaatgcg cgtgtcgggt
    10921 tgaaaaccat tgtcgcttta cctggtgcca tgatgcccaa aggcaatctt attttcccag
    10981 gcgaacttcg tggtgaaaag agttttggta tgatgtgcag tcctcgtgaa ttgcatctgc
    11041 caaatgctcc acaaaaacgt ggggtgcttg aattatcaga agaccaagtt gtcggaacac
    11101 catttgactc agctaaacac tggactgcct aagaagttgt taatatctga tagaccagct
    11161 agaaggaaat aagatggcaa aaaatgttgt gattacagga gcgacatcag gaatcggtga
    11221 agcgattgcg cgtgcttatc tggagcaggg tgaggatgtc gttctaacag gacgacggat
    11281 agacagatta gaaatcctca agtcggagtt tgcagtaagc tttccaaatc aaaccgtctg
    11341 gacttttcca ctagatgtga cggatatggt catggtgaag actgtttgct ctgatattct
    11401 agaaacgata gggaggattg atatcttggt caacaacgcc ggactggctc ttgacttggc
    11461 tccctatcaa gactatgagg agttggatat gttgaccatg ttggatacca atgttaaagg
    11521 tctgatggcg gttactcgct gtttcttgcc agcaatgata aaagtcaatc aaggtcatat
    11581 tatcaatatg gggtcaaccg caggaatcta cgcctatgct ggtgccgctg tttactcagc
    11641 taccaaggct gcggttaaga ccttttcgga tggactgcga attgatacca tcgcaacgga
    11701 tatcaaggtg acaaccattc agcctgggat tgtcgaaaca gatttctcaa ctgttcgttt
    11761 tcatggtgat aaagagcgag ctgcgtccgt ttaccaagga atagaagcct tgcaagctca
    11821 ggatattgca gacacagtag tctatgtgac cagtcagcct cgccgtgttc agattacaga
    11881 tatgaccatt atggccaatc aacaggcgac aggtttcatg attcataaaa aataagaaat
    11941 ttcctcgaaa agttacaaat ttctgtaact ttttttgatt tcctacgaat agataagtag
    12001 gaggaagaaa atatgtataa taaagttatc atgattgggc gtttaacgtc tacaccagaa
    12061 ttgcacaaaa ccaacaatga caagtcggta gcgcgagcaa ctatcgctgt caaccgtcgt
    12121 tacaaagacc aaaacggtga acgtgaagct gattttgtca atatggtcct atggggcaga
    12181 ctagcagaaa ctttggcaag ctacgcaacc aaaggtagtc tcatttccgt tgatggagaa
    12241 ttgcgtaccc gtcgctttga gaaaaatggc caaatgaact acgtgaccga agtacttgta
    12301 acaggattcc aactcttgga aagtcgtgct caacgtgcca tgcgtgaaaa taatgcaggc
    12361 caagatttgg cagatttagt cttggaagag gaagaattgc cattttaata ctcttcgaaa
    12421 atctcttcaa accacgttag ctttatccac aacatcaaag caatgctttg agcagcctgc
    12481 ggctagcttc ctagtttgat ctttgatttt cattgagtat aaacattaaa aagtctgagt
    12541 tggtctcagg ctttttatct tgagaaagtc agactttttt cttgattatt tctgactaag
    12601 tgatacaata gaactatgaa ttagcactca ggtataaaga gtgctaataa tatctatctc
    12661 attatggagg aaatcagatg ttgaaaccat taggggaccg tgtggtctta aaagtagaag
    12721 aaaaagaaca aactgttgga ggctttgttc tcgcaggttc agcccaagaa aaaaccaaaa
    12781 cagcccaagt tgtggctact ggacaaggtg ttcgtacctt gaacggtgac ttggttgctc
    12841 caagtgttaa aactggagac cgtgtcttag ttgaagccca cgcaggtctt gatgtcaaag
    12901 atggcgatga aaagtacatc atcgtaggcg aagctaacat cttggcaatc attgaagaat
    12961 agaaggagaa agtaagtatg tcaaaagaaa ttaaattttc atcagatgcc cgttcagcca
    13021 tggtccgtgg tgtcgatatc cttgcagata ctgttaaagt aactttggga ccaaaaggtc
    13081 gcaatgtcgt tcttgaaaaa tcatttggct caccattgat taccaatgac ggtgtgacta
    13141 ttgccaaaga aattgaatta gaagaccatt ttgaaaatat gggtgccaaa ttggtatcag
    13201 aagtagcttc aaaaaccaat gatatcgcag gtgatggaac tacaactgca actgttttga
    13261 cccaagcaat cgtccgtgaa ggaatcaaaa acgtcacagc aggtgcaaat ccaatcggta
    13321 ttcgtcgtgg gattgaaaca gcagttgccg cagcagttga agctttgaaa aacaacgcca
    13381 tccctgttgc caataaagaa gctatcgctc aagttgcagc cgtatcttct cgttctgaaa
    13441 aagttggtga gtacatctct gaagcaatgg aaaaagttgg caaagacggt gtcatcacca
    13501 tcgaagagtc acgtggtatg gaaacagagc ttgaagtcgt agaaggaatg cagtttgacc
    13561 gtggttacct ttcacagtac atggtgacag atagcgaaaa aatggtggct gaccttgaaa
    13621 atccgtacat tttgattaca gacaagaaaa tttccaatat ccaagaaatc ttgccacttt
    13681 tggaaagcat tctccaaagc aatcgtccac tcttgattat tgcggatgat gtggatggcg
    13741 aggctcttcc aactcttgtt ttgaacaaga ttcgtggaac cttcaacgta gtagcagtca
    13801 aggcacctgg ttttggtgac cgtcgcaaag ccatgcttga agatatcgcc atcttaacag
    13861 gcggaacagt tatcacagaa gaccttggtc ttgagttgaa agatgcgaca attgaagctc
    13921 ttggtcaagc agcgagagtg accgtggaca aagatagcac ggttattgta gaaggtgcag
    13981 gaaatcctga agcgatttct caccgtgttg cggttatcaa gtctcaaatc gaaactacaa
    14041 cttctgaatt tgaccgtgaa aaattgcaag aacgcttggc caaattgtca ggtggtgtag
    14101 cggttattaa ggtcggagcc gcaactgaaa ctgagttgaa agaaatgaaa ctccgcattg
    14161 aagatgccct caacgctact cgtgcagctg ttgaagaagg tattgttgca ggtggtggaa
    14221 cagctcttgc caatgtgatt ccagctgttg ctaccttgga attgacagga gatgaagcaa
    14281 caggacgtaa tattgttctc cgtgctttgg aagaacctgt tcgtcaaatt gctcacaatg
    14341 caggatttga aggatctatc gttatcgatc gtttgaaaaa tgctgagctt ggtataggat
    14401 tcaacgcagc aactggcgag tgggttaaca tgattgatca aggtatcatt gatccagtta
    14461 aagtgagtcg ttcagcccta caaaatgcag catctgtagc cagcttgatt ttgacaacag
    14521 aagcagtcgt agccaataaa ccagaaccag tagccccagc tccagcaatg gatccaagta
    14581 tgatgggcgg gatgatgtaa gctttctata gaaaacaact tataaaaaac acaaaaggag
    14641 ggaatgacta acccttcttt ttataatatt cacttctaaa ttgatttgag ctctcctaac
    14701 ttatatgata aaataagact agaaaaagga gaagaacatg atcgatgtag aagaaattct
    14761 gtgcaagatg acccccaatc agaagattaa ttatgaccgt gtcatgcaga aaatggtaca
    14821 agcatgggaa aaaaatgagt agcggccaac cattctcgtg catgtttgct gtgccccttg
    14881 tagtacctat acactagaat atttgaccaa gtatgcagat gtgaccatct attttgccaa
    14941 ttctaatatc catcccaagg cagaatacca taagcgggtc tatgtcacca agaaatttgt
    15001 tagtgatttt aatgagcgga caggaaatac ggttcagtac ctagaagctc cctacgaacc
    15061 caattaatac cgaaaactag ttagggggct agaggaggag cccgaaggtg gcgaccgttg
    15121 caaggtttgt tttgactacc gactggataa aacagcgcaa gtggctatgg acttgggctt
    15181 tgactacttt ggttcagcct tgaccatcag tcctcataag aattctcaaa ctatcaatag
    15241 catcggaatc gatgtgcaaa aaatttacac gccccactat cttcccaacg atttcaagaa
    15301 aaatcaaggc tacaaacgtt cagtagagat gtgtgaggag tatgatatct atcgtcaatg
    15361 ttattgtggc tgcgtctatg cagcccaagc ccagaatatt gacctggttt aagttaagaa
    15421 ggacgccaca gcatgcttgt tggataagga tgttgagaaa gactattctc atatcaaatt
    15481 tatagtagat tgaaactaga atagtacacc tttacttcta aaacattgtt agaaatcgat
    15541 ttgactgtcc ttatttcatt ttactatact gttacgaaat tagatatata aatgaaaact
    15601 tgcctcaagg taggtttttt gctagtagaa aagcgataga cagaaattgt tggactctta
    15661 aaaaaatgaa gttttgctca aaagagcttt ttaaattaaa acgagcaatt atcagttcaa
    15721 atctgtttca aaagaagaac ggataaatta atcgatgctt tgtttttgag ttaggaagtt
    15781 aagcatgcca aactgagtcg taggaataaa tttctttata gaaatgtgtt acaactctgc
    15841 tataacggaa tattcctttt ttgcatttat ataacttatt taacactatc tttattcttg
    15901 tgatataatg ccaaattata gagtggaggt catttaaaat gattgataaa gtaattgaac
    15961 aatataaaag tcatgataat cttgatattc gagtagaatt gcataagaag t
//
LOCUS       DAVGJJ010000041        15926 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000041 DAVGJJ010000000
VERSION     DAVGJJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.041"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1104)
                     /locus_tag="VVE35_001212"
     CDS             complement(<1..1104)
                     /locus_tag="VVE35_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3061404.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYK"
     gene            1162..2328
                     /locus_tag="VVE35_001213"
     CDS             1162..2328
                     /locus_tag="VVE35_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU3061405.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSASEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            2466..2792
                     /locus_tag="VVE35_001214"
     CDS             2466..2792
                     /locus_tag="VVE35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU3061406.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            2779..3372
                     /locus_tag="VVE35_001215"
     CDS             2779..3372
                     /locus_tag="VVE35_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU3061407.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3365..4276
                     /locus_tag="VVE35_001216"
     CDS             3365..4276
                     /locus_tag="VVE35_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU3061408.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKSESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4339..5403
                     /locus_tag="VVE35_001217"
     CDS             4339..5403
                     /locus_tag="VVE35_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061409.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5581..6441)
                     /locus_tag="VVE35_001218"
     CDS             complement(5581..6441)
                     /locus_tag="VVE35_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU3061410.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWLIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(6563..7549)
                     /locus_tag="VVE35_001219"
     CDS             complement(6563..7549)
                     /locus_tag="VVE35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU3061411.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7821..9296)
                     /locus_tag="VVE35_001220"
     CDS             complement(7821..9296)
                     /locus_tag="VVE35_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU3061412.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9554..10477)
                     /locus_tag="VVE35_001221"
     CDS             complement(9554..10477)
                     /locus_tag="VVE35_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU3061413.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10491..11420)
                     /locus_tag="VVE35_001222"
     CDS             complement(10491..11420)
                     /locus_tag="VVE35_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3061414.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11739..11960)
                     /locus_tag="VVE35_001223"
     CDS             complement(11739..11960)
                     /locus_tag="VVE35_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061415.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(11914..12066)
                     /locus_tag="VVE35_001224"
     CDS             complement(11914..12066)
                     /locus_tag="VVE35_001224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061416.1"
                     /translation="MQTVKDAIASLESIKESQSATKDGGQTAGKETAGEDESANQTQE
                     ISQGIK"
     gene            12254..12391
                     /locus_tag="VVE35_001225"
                     /pseudo
     CDS             12254..12391
                     /locus_tag="VVE35_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(12495..12650)
                     /locus_tag="VVE35_001226"
                     /pseudo
     CDS             complement(12495..12650)
                     /locus_tag="VVE35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(12835..13446)
                     /gene="rpsD"
                     /locus_tag="VVE35_001227"
     CDS             complement(12835..13446)
                     /gene="rpsD"
                     /locus_tag="VVE35_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU3061417.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(13641..14693)
                     /locus_tag="VVE35_001228"
     CDS             complement(13641..14693)
                     /locus_tag="VVE35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU3061418.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(14690..15388)
                     /locus_tag="VVE35_001229"
     CDS             complement(14690..15388)
                     /locus_tag="VVE35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061419.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(15555..>15926)
                     /locus_tag="VVE35_001230"
     CDS             complement(15555..>15926)
                     /locus_tag="VVE35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3061420.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMVASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT         4684 a         3425 c         2943 g         4874 t
ORIGIN      
        1 cttgtactcc ttcttccatt taaatcctct aattaaacct acaatcgtcc caaagccata
       61 agcaaagtga atggaaaata aaagaaaggg catcacaatt agaaatccat ttttatgttt
      121 taataaagtc agcaaagtga gtagtgacaa aagtagaaaa taggcaccta atagtaaagt
      181 tatgaatacg aatgtgatcg gtaacaatgc tagactaaac acaagactca aaacaaataa
      241 acaaggaaca tagtgaaata atgataaaca cttaaactga acatgacttg tcaagccaat
      301 ccacaaacca tttaaatact tttgatgcag cattttcttg aatgttggtc gaatatactg
      361 ataagataga atacttgggc tatagcggat tttataacca tattctcgaa ttctataatg
      421 aatatcatta tcttcagttc ggccaagttg ctcatttact aaaccaacct tctggaaaac
      481 ctctcgttta tacattccat gaaaaataga agaaacatat ctatcctcag aactatttcg
      541 ataattggca atgctactgc caaacatatt ttcctcaaca agatgcaagg tctctgccca
      601 ttttcctttt ccttcgacaa tcgtcggtct aggcccccca cagacaaatt caccttgttg
      661 aataatagcc acattgttca ttacaaaagt ctcagtaact tttgaatgag catcaatttt
      721 taaaataagg tcccctacag aatgtttaac tcccaggtta aaaccactag cttgattttt
      781 cttaggattg ttatacaatc taattgagtt aaactctgta tcttccttta taaattgctg
      841 aatgatagct gtggtcccat ctgtggacat agcatttata aatagaattt caatattctc
      901 tttaggatag gtttgatttt ttaagtcttc aattagacca ggcagatatt tttcttcatt
      961 gtatgcactg attacaatgg atacgagaag actttcaaca ttttgagtca ctactactct
     1021 cctgaatttt tccttccacc attctatcat aattttcaag actttcccat ttttatcttg
     1081 aaagccagaa accttacttg acattatagt gaaaaagcct taaaataaac tgttattaaa
     1141 atcagctaga agaggtatta tatgaaaaag caatcactct tttttgttcc aggtattatc
     1201 ctgattggtg tttccttgcg aactcctttt actgttttac ccattatttt gggaaatatt
     1261 tcgcaaggtc tggaggtaga agttagttcg cttggtgtct tgaccagcct gcctctcctt
     1321 atgtttaccc tcttttcacc attttctacc caactggctc agaaaatcgg cttggagcat
     1381 ctcttcacct acagcctctt cttcttgacc atcggctcac ttattcgact aatcaatctg
     1441 cccctgctct atctaggaac cttgatggtt ggggcaagtg tcgcagtcat caatgtcctg
     1501 cttcctagtc ttatccaagc caatcaacca aagaaaattg gttttctgac caccttatat
     1561 gtaacgtcta tggggattgc aacggctctg gcttcctatc tagctgtgcc cattacacaa
     1621 gccagttctt ggaaaggact tatccttctc ctcacgttac tctgtctagc aacttttttg
     1681 gtctggctcc caaatcaccg ctataatcat agactagctc cacaaaccaa acaaaaaagt
     1741 caaataaagg tcatgcgtaa taaacaggtt tgggcaatta ttatcttttc aggttttcaa
     1801 tccttgatct tttacaccgt catgacctgg ttacctacca tgtctatcca tgcaggtcta
     1861 tccagtcacg aagctggctt gctgacttct atcttatctc tgattagcat tcctttttca
     1921 atgaccatcc caagcctgac aaccagttta tctactcgca accgtcagct catgctcact
     1981 ctggtttcac tagctggtgt ggtcggcatt tccatgctct ttttcccaat caataatttc
     2041 atttactggc ttgccatcca tctcctcatc ggaaccgcaa ccagtgccct cttcccttat
     2101 ctcatggtca acttttcact caagacaagc gcctctgaaa agacagccca attgtccggc
     2161 ctatctcaaa caggaggcta tatcctagca gcctttgggc caaccctctt tggttacagt
     2221 tttgacctgt tccactcttg ggtaccatct gtagctgccc tcttgctcat cgatatcctg
     2281 atgactgtgg ccctctttac agtggacaga gcggataaga tcctttaaac ttctagtttt
     2341 agctagaagt ttttatatat aaaaatttta cacatttctc ttgacagggc tttcttttag
     2401 atgtacaatg tatgtgtaga aaaattatat ataaaaatct tacacattag aaaaggaggt
     2461 ttcccatgta ctttccaaca tcctctgcct tgattgaatt tctcatcttg gccgtactgg
     2521 agcagggtga ttcttatggt tatgagatta gccaaaccat taagctgatc gctaatatca
     2581 aagaatccac actctatccc attctcaaaa aattggaagg caatagcttt ctgacaacct
     2641 attctagaga gttccaaggt cgcatgcgca aatactactc cttgacaaac ggtggtatag
     2701 agcagctctt gaccctaaaa gatgaatggg cactctatac agacaccatc aatggcatca
     2761 tagaagggag tatccgccat gacaagaact gaatacctga ctcagctaga actctatctc
     2821 aagaaactac ctgaagctga ccgtatcgaa gccatggact atttcagaga gctctttgac
     2881 gatgctggag tcgaaggaga agaagaactc atcgctagtt tgggaactcc caaagaagcg
     2941 gcccacgaag ttctatccaa tcttctcgat aaaaaaatca atgaagcacc cgctcaaaaa
     3001 aataaccgac aaattttaca tatcgccttg ttagccctcc ttgcagcacc tatcggcatt
     3061 cctctgggaa tcgccatcct cgtgaccctg ttcgcaattc ttgtagccgc tttgactgtc
     3121 attctggctt tctttgcagt ttccatactg ggtatcatcg gcggattcct atttttagtt
     3181 gaaagtttca ctgtcctcgc ccaagccaaa tcagccttta tcttgatttt tggttctggt
     3241 ttactggcta tcggtgcttc ttcgctagtt ttactaggca tttcctatgt aactcgcttc
     3301 ttcggtctac tcattgttcg tctggtacaa tttgttctta aaaaaggaaa gagaggtaat
     3361 cagcatgcgt aaatggacaa aaggatttct catctttggt gtggtgacta ccgttatcgg
     3421 ctttatcctg ctttttgtag gtatccaatc tgacgggatt aagagcctac tttccatgtc
     3481 caaagaacct gtctatgata gccgtacgga aaagctaacc tttggcaagg aagtcgaaaa
     3541 cctagaaatt actctccacc aacacacgct caccatcaca gactctttcg atgatcaaat
     3601 ccacatttct taccatccat ctctttctgc tcaccatgat tttatcacca atcagaacga
     3661 tagaactctg agtctcactg ataagaaact gtctgaaact ccgtttctct cttctggaat
     3721 tggtgggatt cttcatatcg caagtagcta ctctagtcgt tttgaagaag ttattctccg
     3781 actaccaaaa gggagaactc taaaagggat caacatctca gccaatcgcg gacaaaccac
     3841 catcataaat gctagccttg aaaatgcgac cctcaataca aacagctata tcctccgaat
     3901 tgaaggaagt cgtatcaaaa acagtaaact cacaacgccc aatatcgtta atatctttga
     3961 tacagttctt acagatagtc agctagagtc aacagagaat cacttccacg ctgaaaatat
     4021 ccaagtccat ggcaaggttg aactgactgc caaagattat ctcagaatca tcctagacca
     4081 gaaagaaagc caacgaatta actgggacat ctcaagcaac tatggttcta tcttccaatt
     4141 cacaagagaa aagtctgaat caagaggtac ggaattaagc aacccttaca aaactgaaaa
     4201 aaccgatgtc aaggatcaac tcattgcgag atctgatgat aatattgatc taatatccac
     4261 accaagcaga cgttgacaaa aacgcttaca tctgctacta tggaagcata tttatctaac
     4321 taaaaaggag gttctaccat gaaacaagaa tggtttgaaa gtaatgattt tgtaaaaaca
     4381 acaagcaaga acaagcctga agagcaagct caagaggttg cagacaaggc tgaagaaacg
     4441 atagccgatt tcgatacacc aattgaaaaa aatactcagt tagaggagga agtctctcaa
     4501 gctgaagtcg aattggaaag ccagcaagaa gagaaaattg aagctcctga agacagtgaa
     4561 gcgagaacag gaatagaaga aaagaaggca tctaattcta ctgaagaaga gccagacctt
     4621 tctaaagaaa cagaaaaagt cactatagct gaagagagcc aagaagctct tcctcagcaa
     4681 aaagcaacca cgaaagaacc acttcttatc agtaaatctt tagaaagtcc ttatatcccc
     4741 gaccaagctc caaaatctag ggataaatgg aaagagcaag tgcttgattt ttggtcttgg
     4801 ctagtggaag cgatcaaatc tcctacaagt aagtttgaaa caagtatcac acacagttac
     4861 acagcctttc tcttgctcat tctgttttct gcatcttcct ttttctttag tatctatcac
     4921 atcaaacatg cttactatgg acatatagca agcattaaca gtcgcttccc tgagcagcta
     4981 gctcctttaa ctcttttttc tatcgtctct atcctagtag cgacaacact cttcttcttt
     5041 tcattcctct tgggtagttt cgttgtgaga cgatttatcc accaggaaaa ggactggacg
     5101 ctagacaagg ttctccaaca atatagtcaa ctcttggcaa ttccaatctt cctcactgct
     5161 attgctagtt tctttgcttt ctttgatagc ctacgattta cagctctctt gtgtgtgatt
     5221 agcattggaa tcattctgct tgccagtctc catatcatta caagaccaag tcaagcaagt
     5281 gaaaccgact ccttctatca attattcttg tctgtccttg tgaacggagt tattatcctc
     5341 ctcttctttg tagctgaagt ggcactgatt ggagattatc ttcgtatctt ggcctttctt
     5401 taaacttcat actcttcgaa aatcaaattc aaaccacgtc agcttcgcct taccgtactc
     5461 aagtacagcc tgcggctagc ttcctagttt gctctttgat tttcattgag tagcaatcct
     5521 gtcatccttg gcaggatttt ttctatgtca aaaaagcaag tcgattgacc tgcttctgct
     5581 ttactcttct tgtgctaatg ctttaaaatc tttgtctaat atgggttgca cctctatttg
     5641 attggcgtct agttggtggt aaaaagatag gggtaatacc tctaagaatt ttgcatagtc
     5701 caagcgggca atggcttcat agtcttctgg tttagagcca tctcctgtga tttgaaccaa
     5761 gtcaatctcc cactgcactt ccttatccac caattgctca atataggctg caggaacaaa
     5821 cggttctctc ggtaaaactg tcttgactcc ttgagctaga tggtaaacgc catgcacttt
     5881 tccttctcca tcctgataga aggaaactct cgcaaagtag gcatctgtct cttgaaccgc
     5941 acgcacacgc gcttcaaact gagccaagcc atctgcctct aaaaagcttt tcaagttctc
     6001 acgactaaag aaaataggat taaaaattcc ttggcaaatc gtaaaacgag ctacagggtc
     6061 tgctagatag gcttgaatac ttgcttggaa ataagcttca aaatcaaaac catctagccc
     6121 ttcaatatca gtccctgtat aagcaagcaa agcatctaaa aatgacttga taatcagcca
     6181 gtctgtcttt gttagtaggt caggaagatc gacacggtaa gccttttgac catcagcata
     6241 actgacctta aaaagaaatt gtgattgccc cactatcgca cactcaatat attcgagacg
     6301 attaagaggc tgacggagat agactacatc atagctatgt gactccaaac cgtctagcaa
     6361 ggccaagata gacttggcat tcaaaatctc ttgttctcct aaaatgctct gtttatttgg
     6421 aataaaaaat gttttcgcca taactggact cctttgaaat cgtttacata cagtatacct
     6481 tatttttctg aaagatacaa aaacaaactt tagatttttg agtacaaagc atagaaaaac
     6541 agcccctaag gactgtttga tcttatacag tagctgcctg atccaagctt tcaccgatag
     6601 cggctaggcg ctcaatcact tcagcttgtg tcaattcatt ttttgaaaca tagcggttac
     6661 gtgggtgaac acggcactcg tgtgagcatc cacgaaggta cttgtcttca ttttcttctg
     6721 atgtcaagat acgacggtta cagaatggat ttccacagtt gacataacgt tcacatggcg
     6781 ttccatcaaa ccagtctttc cctacgatag ttgggttgac atggttgaca tcaacggcaa
     6841 tacgctcgtc aaagacgtac attttcccat cccaaagctc accttgaact tctgggtctt
     6901 taccgtaagt tgcgattcct ccgtgcaatt ggccaacatc tttgtaacct tcacgaacca
     6961 tccagcctga gaatttctca cagcgaacac cacctgtaca gtaaaccacg acacgcttgt
     7021 ccatgaattt ttctttgttg tcacggaccc attgtggtaa ctcacggaag ttgcgaatat
     7081 ctgggcgaat agccccacgg aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa
     7141 ggacaacggt atctttatca agaagcgctt ctttgaactc ttttggagac aagtaagcac
     7201 ctgttgtttc aagtgggttg atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga
     7261 caatttcttt cttgtagcga acaaacatct tcttgaaggc ttgttcactt tcttcgtcaa
     7321 tcttgaacca gagatcttcc attcctggaa ggctgtgaac gtagtccatg tatttttgag
     7381 ttgtttcata gtcacctgaa actgttccgt taattccctc gtcagcgact aggatacggc
     7441 ctttaaggcc gattgattta cagaaagcca agtggtctgc agcaaattgc tctgcatttt
     7501 caattggagt ataaaggtag taaagtaaga cacgaatatc ttttgccata agatttgttc
     7561 tcttttctat tcttaaattt tctaaatttt ttatttatcc atactagtat acctgcttga
     7621 gaaaacgaat gcaatttatt tgactggaaa ttgtagaggg ttctcgaaat ttcctgaatg
     7681 gttaaaataa ggacaagaga aaacatggat atctatatcc ttgtgccaaa aaaaccactg
     7741 ccctccccag accaacctga ggaaagcagt gattcttatt ttaggagtta ggaatgaata
     7801 cacgaaatca atttagctga ttattttttg tttttcaaga attcatcgta ttgtttttgc
     7861 atttcgttca atactttttc gtaggcacct tcagatttca atttttccat caattctgga
     7921 atcgctttat ctgggtctac agtaccagtg ttgatagctg tatcaaattg ttgcattgtg
     7981 ttagcaatag ctgagatttc agatttcaca ttgtcagtat tgaagataaa tccaagcgct
     8041 ggagattctt tagcttctgc caattctttc ttagaatttt cgatttgttg gtctgtaacg
     8101 ttttcgttga tgtaaaggat ccagttgtta ccagtgttcc atccacccat gtgagtgttt
     8161 cctttgtagc catcaagaac gcgaacacgg ttttctttac cttcaatttt ttcccagttc
     8221 ttgccttctg gaccgtaaac aagaccgttc aagagttctg ggttcgtatt caagaggttc
     8281 aagatttcca ttgatttttc tttgttctta gagttgtttg agatgacaaa gttagcaact
     8341 tgtgttgttt ggtttttctt gatgaagtta gtaattggtt tgatttggat atctttgttg
     8401 gcaacacgtg aaagcaagct gttaccgtag tcagctggtc ctactgtttc ttcacgaacg
     8461 aaccaagtat cttgttgaag gtcaaatgaa gtatcgcttg ttgcgacatc ttttggaatg
     8521 tagccagctt catagaattt gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg
     8581 taacggttta caactttagt agtatcgcct tcaagatcaa taacgaatgg aagcccgttt
     8641 gctactgggt agtcaaagtt atcagatggg atgaaaactt taccaatagc aaatggtact
     8701 acgtctggag ctttttcttt aatttgtttc aagactggct caagagtttc gtaagaagta
     8761 acacctgaaa tatcgatacc atatttggca aggagagttc cgttgaaggc aaagttttga
     8821 gatgatgcaa cgttggctgc aactggaaca gcgtaaattt taccgtttac agtattaccc
     8881 ttgatgtaag ctgggtcaag tgccttgtaa aggtctttac cttctttttt gtacaattct
     8941 gtcaagtcag cataagcacc tttttgagca tttacaatat agttatctgc aaaggcgata
     9001 tcatagtttt caccagatga tgtgataact gacattttct taccatagtc accccagcca
     9061 aggtattgga tatccaattt agcaccaact ttttcttcga tgattttgtt ggcatttgct
     9121 aacaattcat ccaagttatc tggtttgtca ccgatttggt acattttgat aacaggtttg
     9181 tcacctgaat cagcagcttt tttgctgtta cctgtcaaat ttccacaagc agcaagacct
     9241 gcagccagag cgactacact agcagatgca aaagcatatt ttttccagtt tttcatgata
     9301 aaaactcctt tttttatttt taaacttata aacaatgtaa tgatcttata ctcaatgaaa
     9361 atcaaagagc aaactagaaa actagccgca ggctgctcaa agcactgctt tgaggttgta
     9421 gataagactg acgaagtcag ttacatatat ctacggcaag gcgacgttga cgcggtttga
     9481 atttgatttt cgaagagtat taacttcaca caagggaagt tgggaactga gaaatgttat
     9541 ttctcaataa gcactattct ttcacaccac cgatagtcaa accttttaca aagtagcgtt
     9601 ggaagaatgg atacaaaatc gcgattggaa gggttgcaac cacaaccatg gccatacgac
     9661 ctgtttcttt cggtagagca actcccagtt gaccagtcaa gccgaccgct ttggcaatgt
     9721 agtccatatt ttgttggatt tgcatgagca aatattgcaa tggatacaag ttgtcactct
     9781 tgatgtaaag aagggcgttg aaccagtcat tccagaaacc aagagctgtt aagagcgtga
     9841 tggttgcgat acctggtagt gacaatggca aacagatttg gaagaaaatc cgggcctcac
     9901 tggcaccatc gatacgagcc gattcgagaa tggcttctgg aatggtcttc ttgaagaagg
     9961 aacgcatcaa gatgatgtta aagggtgaga gaagcattgg cacaatcaag gcccaaacag
    10021 tgtcaccaag ctgaagtaca cgggtcacca tgatataacc tggtaccaaa ccagcgttga
    10081 acaacatact gagaaggacg aagatggtaa aaaatctgcg atacttaaag gttgtccgtg
    10141 aaatagcgta ggcataggtt gttgtgataa agacatttgt caatgtccca actacggtta
    10201 caaagacaga gatgaagagg gcttgtagga ttttatcctt aaactgtgcc aaaaactcaa
    10261 aaccgtctaa gccaaattgg gatgggaaga agctatagcc gtattggagg aggcttttct
    10321 cgtctgtcac tgaaataatg ataacgaata caaatggtag gatacaagag agggcaatca
    10381 aacccgaaat gatactgaag aagatatctg ctttcttact gaaggagtga atgccgacat
    10441 tatcaatttt ttctttttta attttctttt ttgccatatt ctcctccttt ctagaacaaa
    10501 gctgagtttg gatcgactcg tcttgcaagc aagtttgata ggataaccag aatcaaacca
    10561 acaacggatt ggtaaagacc ggctgctgca gccataccga tatctgctgt ctgagtcaaa
    10621 ccattaaaga catatacgtc caaaacgttg gttacattgt aaagctgacc agcattgtgt
    10681 gggatttgat agaagagacc gaagtctgcg cggaagatat ttccgactgc aaggatggtc
    10741 aatacagtta caagcggagt caactgagga atggttacgt tgcgaatacg ttgccacttg
    10801 ctagctccgt ccactgtcgc tgcttcgtag taggttggat caattcccat gatcgtcgca
    10861 tagtacatga cactgctata tccaaagcct ttccaaatac ctaggaaaag taggagatag
    10921 ggccagatgc ccaggtcagc gtagaaattg acttctttga gaccaagact ttccaataga
    10981 tgattgaaca cccctttatc aatatttagg aaggcatctg taaagaaact aatgataacc
    11041 caagacaaga agtaagggaa caacatagaa gtttgaaaaa tcttcaccat tctcttagaa
    11101 cggagctcgc tgaggataat ggcaatccct acagatacaa ctaaacctag aaagataaag
    11161 ccaagattgt agaggacagt atttcgtgtg ataataaagg cgtctcttga actaaataag
    11221 aatctgaaat tatcgagtcc gacccattta ctatttatga tactatctat gaaaccatta
    11281 ctggtcatgt ggtagtcttt gaaggcaacc acgttcccaa atactggaat gtaaaagaat
    11341 agaatcaacc agagtgcccc tggcaaaacc atcaagagaa agatccagtt gtctctcaat
    11401 gtttttgaaa actttttcat aatttcctcc ctttttattt tgatatccat ctaaaaattc
    11461 ttttttagac ttttgataac gattacatta ttagtatact cctatttgca ggttaggtta
    11521 aactcctaat tatagaaaaa actccacaaa ttatgtagca gatttaaaac tttatcacca
    11581 ctatcaaaca aatgtcctaa atcaattgtt tattttatct ctattagccc agtgatggcg
    11641 tcactctgtt ataagcatcc aacaacgggg tatactgaaa aatctccaga ctagggaact
    11701 cagagatagt tcctaatctg gagattttta atatgttatt aggcgtttgc tttcaactta
    11761 gcaataacct ctttaagatt atcaatcaac tctgctgcag tatgctcaga gcctttttca
    11821 tctgccaaga acaaaactgc tttttgaagt tcttcttgag agttttcaag gacatcctta
    11881 tctactgttt caaggtttga gtctttaaga agtttactta attccttggc taatttcttg
    11941 agtttgattt gcagactcat cttctcctgc tgtttctttg cccgctgttt gtcctccatc
    12001 cttagttgct gactggcttt ccttaatgga ctctagggaa gcaatggcat ctttgactgt
    12061 ttgcaagata tcacgtaaac cttactctgt caaactatca tctgcaaaag ctttcttagc
    12121 ctctgccaaa accagacgtg ctgaatctgt ggtaggattc gatacacctg tcaatgatct
    12181 caaaagattt tctaaggttt gagtctgctt actaatacta gactaaaatc aaaaagcatt
    12241 atataacagt gatatgaaat caactaaaga agaaatccaa accatcaaaa cacttttaaa
    12301 agactctcgt acagctaaat atcataaacg ccttcaaatc gttctatttc gtctgatggg
    12361 caaatcttat aaagagatta tagagctttt atagtgattt gaaataagat gtgaacaact
    12421 ctatcaggaa agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata
    12481 gattcaatac actatacttg gtggtttagc tctcctgttt tctcttttag ctttaaccag
    12541 ccataaatgg tattacgtga gatttggaaa acgtgtgatg cttctgttat actatctgtt
    12601 cgctcacaat aagagagaac ttttttacga aaatctattg aatattccat aaaagattat
    12661 accacattgt gtactatttt tggttcattt tactatatca ctttggacta tatcggtcta
    12721 gttatgtcta aacgattata gctggatact aagagaaatc aaaaaagcac tctaggatag
    12781 aggcctaaag tgcttagttt caaggcttta cagcctatca tatttaataa aatattacaa
    12841 catcttgttg tagaattcaa cgacaagtgc ttcgttgatt tctgggttga tttcgtcgcg
    12901 ttctggcaag cgagtcaatg aaccttccaa tttttcagcg tcgaatgata cgaatgctgg
    12961 acgtccaaga gtagcttcta ctgcttcaag gattgctgga actttcaatg atttttcacg
    13021 aactgagatc acttgacctg gagttacgcg gtatgatggg atatcaacgc gtttcccgtc
    13081 aacaaggatg tgaccgtggt ttacgaattg acgagcttga cgacgagtag tcgcgagacc
    13141 aagacggtaa acaacgttat ccaaacgacg ttccaaaaga agcataaagt tgaaacctag
    13201 gattccgcct ttgatttttg tagcttgtac gaacaagtta cggaattgtt tttcacctac
    13261 accgtaagtg aaacgaagtt tttgtttttc agccaattgc aaaccgtatt ctgacaattt
    13321 agaacggttg tttggtccgt gttgtcctgg tacgtagtta cgacgtgcca attctttacc
    13381 tgtacctgta agtgaaaggc caaggcgacg agcttgtttc caagatggtc ctgtataacg
    13441 tgacatatga atgtcctcct gatataaata atatttcggt ggaaatagtc acttagaaag
    13501 ccctgattcg tgcagatgcc cttcgcctaa acagccaagg ttacttgtca taagacacct
    13561 gttgacgagc ttcatgcttt cctgctgata tttcacacaa aattcattct accatgaata
    13621 gctggatttg taaaggggtt ttaggcttta ttttcactac cagagagatt gagaacgagg
    13681 gtaaaggtac ttcctagacc gtactggctg ctgactgtga tttccccacc caattgatgg
    13741 gccaattcac gcgcaatcgc aagtcctaat ccatgaccac ctgtcttcat gttacgcgaa
    13801 gtttcgacac gataaaggcg tttgaaaata ttttccaaat cctctggggc aatgccctgc
    13861 ccttcatcgg tcacactgat tgaaagctgg cccttctcca gcttagccac cacttccagc
    13921 ttggttcctg gagcagaata tttaaaagcg ttatcgacca gattcaccaa gatacgagaa
    13981 agcttagcat aatctccctc aatccgggca gactctggga ttacctgcaa gtggacatct
    14041 cttctctcct gctcaatcaa aaactgaaat tcactcatgc actcaattaa gagcttgtcc
    14101 agaaaaatac tgtctttact ggtagtttcc acctgatttc tagctgtgtt tagggtcaaa
    14161 aaattcaact cctcaaccag tttattgagc ctctccgtct ggcgtccaat ggttgctaga
    14221 taatgagctt gctccgactc cttgataatc ccatccaaaa tcccttctac cgtcgcttgg
    14281 atcgaagtga taggagtctt aatatcatgc gacaactggg caatcatcaa gcccttttct
    14341 cgttcgcttt cttccaagga atcaaagctt acctgcaaat catgggacat ctcattaaaa
    14401 gtttgcccta attgctgaaa ttctacagga ccttgaacct ccaaatttga aggaaaatcc
    14461 ttggccgcta cccgcttggc atgctccttg agtttgccca acgacgtaaa gactggcaat
    14521 aggagaaaga gactaatccc agcaccgaca aggctggcaa cgatggtcat cccaagcaaa
    14581 aagtaaatct cgcctttcgc aatcagcatt ttttgaacag cccaaaaaac aaccaaaatg
    14641 gttaagaggg ttgaaataat atatccaacc aaaatataac tttttagttt catgtttgtc
    14701 ctctcggttt ctctatctta tatcccaacc cccaaactgt cttaatagtg ggcgtttggt
    14761 cactactata ttttgccagc tcctgtcgaa gagcatggat atgcacattc aaggtattgg
    14821 tgtcatccac gtagtcttct ttccagatct tttcatagag gtctgtcttg gagaaaactc
    14881 gctctggatt gctagctaaa atccacagca attcaaatga tttaacagtt aaatccagca
    14941 tttcttctcc tatttgaact tcatgactac tatgattcat ttttagattg ccaagggaaa
    15001 tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat attgtggaca cgcaaaacca
    15061 gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc tcccaagctc aggccgtaaa
    15121 tcttgtcctg ttcactggtc ttagcagtaa taaataggaa aggctgctct ggtgataagt
    15181 attgaacctc actgattaaa tcataaccat ccatccgagg catcatgaca tctgtgataa
    15241 tcaaatcaat cggttttttc ttgaacagct ctagcgcttc cagtccatca tgggctacca
    15301 agacctgata acctgcctga attaagtatc tctgatgaat atctgtgatt tctacctcgt
    15361 cgtcaacgag taaaattgtc tttcccatct gtctctcctt tgataaaaac agtgctatac
    15421 tgctttaagt ataacactat ttttattgtt ttacgatgat tttaatctgc tattgaaaga
    15481 aagtcctgat tgctgattga gttgttaaac taatcttagt cagcccacta tccatcattt
    15541 atttccttag ctgattagtt ttcttctttt ttgtttttca aacctagacc accgagtaaa
    15601 cctgcaagcg caagcccaag gaaaccaata cttgccattg atgtttgagt ctcaccagta
    15661 tttggtagca tagctttatc ctctgacatc atcgtatcag acatcttgtt agcagaagca
    15721 accatgtttt cacctgccat cgtgttggta gaacttgtca tggtgtcagc aggcatgcta
    15781 tctgtagtac ctgtagcatg attgtgattc atcggagtca cgccagaacc agagttagaa
    15841 ggtgataatg aaccatttgc tgtgtctgat gtttctttaa catttatctt aatagtgact
    15901 tttttagttg ctacgatgtt gtccaa
//
LOCUS       DAVGJJ010000042        15573 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000042 DAVGJJ010000000
VERSION     DAVGJJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.042"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..508)
                     /locus_tag="VVE35_001231"
     CDS             complement(<1..508)
                     /locus_tag="VVE35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3061421.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLL"
     gene            complement(521..2014)
                     /locus_tag="VVE35_001232"
     CDS             complement(521..2014)
                     /locus_tag="VVE35_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU3061422.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            complement(2522..3688)
                     /locus_tag="VVE35_001233"
     CDS             complement(2522..3688)
                     /locus_tag="VVE35_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU3061423.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDHKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(3701..4440)
                     /locus_tag="VVE35_001234"
                     /pseudo
     CDS             complement(3701..4440)
                     /locus_tag="VVE35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(4462..5275)
                     /locus_tag="VVE35_001235"
                     /pseudo
     CDS             complement(4462..5275)
                     /locus_tag="VVE35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5412..6626)
                     /gene="thiI"
                     /locus_tag="VVE35_001236"
     CDS             complement(5412..6626)
                     /gene="thiI"
                     /locus_tag="VVE35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU3061424.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(6635..7777)
                     /locus_tag="VVE35_001237"
     CDS             complement(6635..7777)
                     /locus_tag="VVE35_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3061425.1"
                     /translation="MIYFDNSATTKPYPEALETYVQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            7915..8340
                     /locus_tag="VVE35_001238"
     CDS             7915..8340
                     /locus_tag="VVE35_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061426.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(8491..10716)
                     /locus_tag="VVE35_001239"
     CDS             complement(8491..10716)
                     /locus_tag="VVE35_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU3061427.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     TENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNAQSEYKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     INENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(10880..12841)
                     /locus_tag="VVE35_001240"
     CDS             complement(10880..12841)
                     /locus_tag="VVE35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU3061428.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATAATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLG
                     NLGSYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFG
                     KIPYAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLP
                     LFGTILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNK
                     AAYVFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTN
                     IIMGLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALT
                     SNALLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(12838..13749)
                     /gene="pfkB"
                     /locus_tag="VVE35_001241"
     CDS             complement(12838..13749)
                     /gene="pfkB"
                     /locus_tag="VVE35_001241"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU3061429.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(13746..14486)
                     /locus_tag="VVE35_001242"
     CDS             complement(13746..14486)
                     /locus_tag="VVE35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3061430.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            14648..14735
                     /locus_tag="VVE35_001243"
     tRNA            14648..14735
                     /locus_tag="VVE35_001243"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14682..14684,aa:Ser,seq:gga)
     gene            complement(14905..15252)
                     /gene="ssrA"
                     /locus_tag="VVE35_001244"
     tmRNA           complement(14905..15252)
                     /gene="ssrA"
                     /locus_tag="VVE35_001244"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4584 a         3445 c         2887 g         4657 t
ORIGIN      
        1 cgagcaagtt tagttcatct aactgaaatt ttcttttagt aataagcctt ttaatcgtat
       61 taagaataca gataatgttc tcttgttctt cgatacctag caattctaat tgtaaatcaa
      121 gtaatatatt cttgtttaaa tgaggaattg ttgcacctgt tgaatgatct cgtaaatact
      181 gcgatttact ttccaaaaag actcccaagt aatctgatat aattttttct ttgtatcgct
      241 cattcttttt taagaccgta attgtactac caacagctcc cgataatcca taaccaactg
      301 ttcctgcatt agctccatcc catgctatca gaatatcatc tgggagtgct tcagtcatat
      361 ttaaactttc agtgaatttt aaattattat tatttcttaa atcatctatt tgaatataac
      421 gttggcttag agttgtttgt tcagcaagta cagtggcttt cttgcctttt tttagagata
      481 agacttcccc caacttcact tttttcatac agccacctcc ctacttgagt aatttttcca
      541 attcagccaa gccagcttga atttcttttt ctaaatcatt gattttcttt aatatgactt
      601 ctgttggttc atactcaact ttttcatact caatctcttt atatttattg atagacaaat
      661 cataatcatt ttcctttatc tcagcaactg gaacaaagaa agattgatcc gttctctgac
      721 gttctgcttc tttttcaaga tgatgaaagc gttcgataat atctggaata tcattgtcgc
      781 taatcggttg tcgcttatca tccaaactta aaccatccgc tttcatatcg taaaaccaga
      841 ctttgtcagt accaccatta ccagtttttg taaagatgag aatggcagtt gaaactccag
      901 cataaggctt gaacacacca ctaggcattg agattacagc atcaagctta tgattctcta
      961 caatttcctg acgaattcct ttatgagctt tagacgaacc aaaaaggaca ccatcaggta
     1021 cgataactgc tgctcgtcca cctggtttta aagttcgcaa gaaaagagaa agaaagagta
     1081 attctgtttt tttggttttt acggttgcaa gaaggtcatt agaggttgaa ttgtagtcaa
     1141 gtgagccctt aaaaggagga tttgctaaaa ccaaagtata tttatcggct tcttcattat
     1201 cttgagacag cgagtcaagg taactgattt gtggattttc tactccatgt agcatcatgt
     1261 tcatcgcccc aagtctcaac atagtcgtat ccgtatcatt tccatgaaac atctgattat
     1321 gaaaatgatt gatattatct gtattggttt cccattcatc tttcttacgc tttaagtaac
     1381 ggctagcaga tactaagaag ccagcagaac ccatagcggg atctgagatg atatctttga
     1441 tagtcggttg catcaactca accatcatat cgatgatgtg acgaggtgta cggaactgtc
     1501 catttttacc tgcggtcgac aattttgata acagatattc atagatatct ccgatatcag
     1561 taatactttg tttgtcatta tcaaaatcta catctaatcc cctagttgga aaaacatcta
     1621 agatagaaat tgccttttga agcgtagcag gtttatttat ttgaaaaata gcttctcgca
     1681 tatatcgtga aaaggctgta tcatctgtat cccccttgag atttttaata aacggaaaaa
     1741 tctcctgagt cattaaacga taaacttcct gagcatctcc tatattttta aaagttgacc
     1801 aacggtattc aggtttatct tttggaaaaa ctccctcata aggaatccct agaaattcag
     1861 catcactttc acgtccaagc tcgacactat ccaaatcttt cataaataag agataagtca
     1921 actgttcaat atttgttaaa ggatttgcgt ttccttctgt ccaaagaatt tcccacagct
     1981 gatcgatttt attttttaat tcgcctgtaa tcatatcttc ccttttcttt agtttttaaa
     2041 acttagtaat atcattattt tatcagattt caaacaaagc ttcaattaac tatatctatg
     2101 cgtcacctga atattttata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg
     2161 ccgtaggtat atgatactga ctctgtcagt tctatccaca acctcaaaac agtgttttga
     2221 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatt aatagacttg
     2281 tttggtatcc tgaaaactat atcgtatagg tgtcgtcatg gatcctttta gctgatttaa
     2341 ggatacgaaa acacaatact atgactcaat tgaaatgtta aaattcaatt tctagccacc
     2401 taatcctgct tcaaatagtt atcgaacaag ttttatgata gctaccttgt taggatgctc
     2461 ttatttttta ccagacaaaa caaaaggatt aagggagtga atttccttaa tccgatagtt
     2521 cttattcttg acgttgtccg aaatcagcaa tcatctgatc catttcttgt cgactcggag
     2581 ttgtcagcat atacaggtaa tctccttgaa gttccgcaaa agcctctggc atgatttttt
     2641 taaccttgaa ctgctggcta tatttggcaa gtaagttctc ctcagaatag acatagtgag
     2701 catttgcacg acgtgatgta gccaaacaat actgaggttc aaactctgac gctgggaact
     2761 cttctcctgc gacaatggct gcatatccac gatagaggtc caaggaatga gcaaagttat
     2821 aaacatcaat ggtgaaacca cctgcagggc ggttattgta ctcaatggta atataatcgt
     2881 ccccctcacg gaagaactca atatggaaaa accgttcttt cataccaaat tctttgacaa
     2941 ttgcttcccc atacttgcgc agtttatgat ccatatcctt gagcacataa taagaattgt
     3001 ccatcttata aatcatgagg tcaagcggtg tataggcgta gtcaaaggtt gttgagaaga
     3061 caatctttcc atccttgtcc acgagcccgt caaaggtaca gatttcgctg gaagtgacaa
     3121 atttttcaaa gaaataaagg gttgaatggt cccattcttg cttgaagtga ttgatatcgt
     3181 cttctgtctc aagtttaaag gttgcggctg ctcccactcc attatcaggt ttggcaatca
     3241 ttggaagacc gatttctttc actgcttgat caacatctgc ttccgtcttg ataacagctc
     3301 caggtaccac aggaacacct gcttttttga aaagtttctt catttcagac ttatatttcg
     3361 tctttttgag atcctctggt ttggcaccaa aaacattgaa ttgttctctg agtgttgcgt
     3421 ctagctcaag ccagtattca ttgtgagact cgatgcggtc aattggacca tgtttataaa
     3481 agagaaaagc aactgcacgt ttgacttcat ctatgttctc aagattatca acacgaaaat
     3541 actcggtcaa gctattgcgc aagggctcat ccaattgctc gtaagactct tgaccaattc
     3601 ccaagactgt gatgccttta ttagctagtt cgatggtaaa ctgttgaaag ttttgtggat
     3661 agtagggaga aataacaagg taattcatag gtaactcctt ttataaatag agattaccga
     3721 ggaaataagg catttgttta cgccaccatt cccagtcatg ggcgacatca tgtccccatt
     3781 cagcaaacca ggctggaatt tgtttcttgt caaaggcttc tttgagcttg taaaaggatg
     3841 gcaaaccatc ttgttcccag gctccaagcc ccgtacacag cacaatctct gcctgacggt
     3901 aacggtcaat aaaccagccg tcgttttggt tccaaatata atctactggc gagttttggt
     3961 aaatagcatc atcgttgtag taatcaccga caaagaaacg tgcgtcgtaa acaccactga
     4021 gagcaatcac tttggtaaag acatctggat gctggaggaa gaaattgagt gcatgatagg
     4081 ctcccataga gcaacctgtc gtcatcatgc catcaaacca acctgtcttg tgcttgataa
     4141 aagaatggcc tcctcaatca cataacgttc gtaggcacgg tgcatttccg cttggtcatg
     4201 agcatttttc caagtagcca accagctctc actatccaca ctagataggg taaagaactg
     4261 gacacggcct tcctcgataa aggaagcaca ggcatcaatc atgccaaaat catagtattc
     4321 gttgtgacta ccacctgatg aagcaaagac cacaactgga atcccaccat gtccataacg
     4381 gttaaggtac atttcacggt taggatgacc actccagtgg ctaagatgtt caatatgcat
     4441 ttcgtttttt cctttcttaa cttaccattt ttctgcaaaa aatctcagac aatctggtag
     4501 attttctgac caagggattt cactatggat ggcaccagac tgaactttta gcacaagatt
     4561 atccagatgt actccccctg ctatcaaatc atggtaatag caaagcgacg agtcgatata
     4621 ggcttgtttg atattgccat ccatcaaggt cttgtctgta tcatctgctt cttctgttcc
     4681 tacatagatg aagatgcgtt ggtcaggcga tagtttctgg cactcgatat agcggttggt
     4741 ggagccagtt tacagatgaa aaaacgccca agcaaccaat tttgtcttgg tattccaaac
     4801 cgataaactg ggtaatattg cctcctagtg aggaaccaat catagccgta tgctggcagt
     4861 ctgcttttgt acgataggtc tcatcgataa aaggcttgac cacctccatg acaaactcag
     4921 catactccac acccttacca ccaaactgct gctctgggat aggagattct tggaacttcc
     4981 aagccgcata ctcattcatt cgccccatac catcattgtc aatagcaacg acaatcatgc
     5041 gactgatatc cggatttcgt ttgatagctg ggataatctt ccatgaatat ccaatgaaag
     5101 actctttgct ataaaaaaca ttttgcccgt catgaaagta tacaacagga taggaacggt
     5161 ctgtatcttt ctcataatct ttaggaagaa gaatacgtac acggcgctcc ttacctgtat
     5221 aaggaacctt gagtttgtgt tctttcattt ttagataaaa gtaggattga ttcattgtca
     5281 gaaaactctc tatattcaaa attttatatc attatatcat gaaaatagaa aaaagttagt
     5341 tttttacctg tttttagaaa aaaaaactaa ctttttttac tgattttcgc tattcttttg
     5401 gattttctga attagagtag attatcaatc aagtcatcaa cttcatcttt ttcggcttga
     5461 ggtgtgattt cagtaatcat aattccagcc actgctcgct caaccaagcc ttcaacatcc
     5521 atacgcgctt cgtactgctc cgcattctta attttaggat ttgtttttgg acgatctggt
     5581 gcaaaaatgg tacaacagtc ttcaaacggt tggattgaaa tgtcaaaggt atcgatttcc
     5641 tgggcgatgt caatgatttc caacttgtcc atggtaacca caggacgaat gatgggagtg
     5701 ttggtaacag cattgatagc cttcatactt tcaagggttt ggctggctac ttgacctaga
     5761 ctttccccat tgatgataac caaaccattt cgtacctcac gaatacggtc agtaatccgc
     5821 atcataaagc gacgagttag agtcatcaaa taagcttctg gcgctttggc tttgatttcc
     5881 tcttgaatct ctgtgaaagg cacctcgata aactggatat ttccgccaaa cttggtcaat
     5941 ttacgggtca agtcctgcgc tttcttgagg gcaccaggac tagtatatgg tggactagca
     6001 aagtgaactg cctcgatatc caccccacgc ttaagagcaa gataacctgc tacaggtgag
     6061 tcaatccctc ctgacaacat gagcatccct ttacctgaag ttccaactgg caaaccacca
     6121 gccccacgaa tggtttcata agaaagatag gctgcttctt cacgaatctc cacctgaaga
     6181 ttgatgtcag gacttttcat ttgaacttgc acatttggaa tggcttcgaa tacagcccct
     6241 ccaagtgttt ggttgagttc acgactatca agttcaaagt tgtggtcgct acgcttgcta
     6301 gaaatcttaa aggtcatacc ttccttgtag atgtcccgca taatctcttg gacagaagac
     6361 ttcaaaactt ctacagattt ttcaacctta taaacaggag aaaagttttg aattccaaaa
     6421 acttgtttga gagattctgc aactgctgtg taatcagctc cattgaggta agcgtgggca
     6481 cggtcgcgat ctgctgttac cttaacttgg gtatagatag acaaaacgtc cgaaatatta
     6541 ttacgaagtt tattgatgaa acgcatacgg tttttaccct tggttgacaa ctctccgtag
     6601 cgaatcataa tttctgaata ctgcatgaat gctcctatct tacttttcta gtttgattgt
     6661 aaatcaattt taacttggtc aaaaactgct cgacctgact catatcattt tccaagtcta
     6721 ggctaagacg cacagctgac ttggccttat ctttgtccac tcccatggca atcaaggtac
     6781 cggctggttt tcctgcctta gatgaacaag ctgaggttgt tgagatgaaa atatcatagt
     6841 cttcaaaggc gtgaacgatg acttcacctc gaacaccttt gattccaaaa gtcagaatat
     6901 gaggtgcaaa gtcttcctca tctgaaaaga caaaaatatc cggatagttc agaagagctt
     6961 ggtgaatcac tgccttcatc tgcccagtct tgctcctaaa gatatctagc ttttccatag
     7021 ataaacggag agccttggct gtcgctgcaa tccctgccac attttcagtt gtcgaacgat
     7081 aatctcgctc ctggccacca cctgtaagaa gaggtgtaat cttcttgcca gatttgatat
     7141 agacaaagcc aacaccacgg acaccatgaa atttatgact cgagaaggtc gcgcaatcca
     7201 cccgttctgt cagatacttt tcagtcggaa ttttggcaag cgcctgaacc gcatcaacgt
     7261 ggaaggaaat agtcggcttg tctgccaaga attctgaaat agcctcaata ggttggatag
     7321 agccgatttc attgttcaca gccatgatgg aaacgaggat tgtatcaggc cgtatcaaac
     7381 ctgctaacgc ctcaacatcg accaagcctt tcttatcaac tggagcaaaa tccacttcaa
     7441 atccttgact tttcaaccag agggctgact ctttgactgc tggatgttca atggctgaaa
     7501 caatgatgtg cttgccaaac tgagcttttt caaaggccac acccttgata acccagttat
     7561 ccccttctgt tccaccagag gtaaagaaga tttcatcgct tttcttaccg attaaatctg
     7621 caatctgttg gcgagaagca tctaagattc gtgttgcttg gtctcccaaa cgatggagac
     7681 tagatgggtt tcctaaaatt tttgaagcga cctgcacata ggtttcaagg gcttcaggat
     7741 aaggcttggt cgtcgctgaa ttatcaaagt aaatcatgtt tctcacgctt tctaaaatca
     7801 ctccctctat tgtatcatga aacggagctt gcgacaagaa agggcaattt ctcttttttt
     7861 aagatttttt taaagaaaca gagtataata agtcataatc aaaggagaga atgtatgtct
     7921 aactatcgta gaacttcaaa accgaaaaca gaacatatca aaaaaggctt tactgttttt
     7981 caaaaaacca ttactactat cggtagtatc cttggtttga ttaccgcggg tatcacgatc
     8041 atgaacgcct tggataataa taaaaatact aaaaaagaac ctacgacaag ccagacgaca
     8101 acctttgtca aagaaattca aaaagaatcc cctcaggaaa atactactcc aaataaggaa
     8161 aataacactt ctcaagaaaa aacacaacaa gaagaaacgc caaaatctag cgtcaaggaa
     8221 gagaaaaaag aagatcagaa aacagcaact caggactcta ctacacctgc tacaagtaaa
     8281 cctgccactg aaaatgaaaa acaacccaat actccaactt cagaaaataa tactcaatga
     8341 aaatcaaaga gcaaactagg aagctagccg taggcagtac ttgagtacgg caaggcaaag
     8401 ctgacgtggt ttgaagagat ttgcgaagag tataaaagta atcaatagcc agtaaaatag
     8461 ctccttccaa ccttggaaag aagctatttt ttattgctgc aatacttttc ttggcttggt
     8521 accttcagct ggaccaatga cacctgccat ctcaagctct tccatgagac gggtcgcacg
     8581 gttaaatcca actgacaaac gacgctgaat catggatgca ctggctttct gtgtttcgat
     8641 aaccaaagac ttagcttctt caaaaagcgg atcaccacca gcatctccat ccgaaaattc
     8701 tccttcattt tcagaaacct cacctggatc aaaactctca tcgtagtctg catctgcctg
     8761 agtcttgatg aagttcacaa tgcgctcaac atcgtcatcc gagataaagg agccttggag
     8821 acgaactgga tgattttcat taatcggttt aaagagcatg tctcctcgac caagaagttt
     8881 ttctgctcca ttttcatcca aaatcgtacg ggagtctgtt cctgatgaaa ccgcaaatgc
     8941 tacacgagat ggaacattgg ccttaatcaa accagagatg acatcaacag atggacgctg
     9001 agttgcaaga atcatgtgga tacctgcagc acgcgccttc tgcccaagac ggatgatagc
     9061 atcttccact tccttgctgg ccaccatcat gaggtcagcc aactcatcca caatcacgac
     9121 aatgagtggt agcggaattt gcttgtactc agactgggca ttgaactctt ctaccttggc
     9181 attaaaacct gcaatattcc gaactcccac cttggcaaag agttcataac ggttttccat
     9241 ttcatccaca accttttgca gagccttgct ggctttgcgt ggattggtca cgactggaat
     9301 caagaggtgg ggaatatcat tgtaaacaga taactcaacc atcttgggat cgaccatcat
     9361 aaatttaact tgatctggtc tcgccttcat gagaatgcta gcaataatgc cgttaactgc
     9421 tactgacttc cctgaacccg ttgaacctgc aactagcaag tggggcattt tagaaaggtc
     9481 aaaagctctt gcggttccat taacagcctt ccctaaagga atttccaaga aattttctgt
     9541 tttcgtttgc gattgttccc atagttctcg gaaagataca gtggcaatat cggagttggg
     9601 cacttcaatt ccgattaggg atttcccagg gattggtgct tcaattcgga catccttggc
     9661 agccaaggct agagcgaggt catctgatag attggaaatg cggttgaccc ttacaccaac
     9721 agccggcttg acttcatact tggtcactga tggcccaatt tcggcccgtt caactgttac
     9781 cttaatacca aagctagcaa aggttgcttc taagattttg atattttctc tgacaatttt
     9841 cttctcttta gactgatctt ttggtttatc tggtgcaaag agttgtaagc ttggaagttt
     9901 gtattcaagg gcttctttgg ctgaaaaatc gacctgaaca tcttggtcat ctgagtcatc
     9961 ttcctgttca gggaatttaa gttcagcttg aggcaggatg atttctggtt ccacccactt
    10021 ttcttctgga ataggtggaa gattttgaac agcttcctct gtcagaattt cacccgtttc
    10081 catatcaaca ggaggcaaat cgagtaaggc tttttcagtc tcttcttgtt ctaatctagc
    10141 ctctttctca gccttttggc gagctttttc ttcttgtttg acaaagcgtt cctcttttcg
    10201 acgctcgtgc ccttcccacc atttagcaaa gcctctactg aaaaattcag caatatcgta
    10261 aacagaccaa gggctgacta ggagagaacc cactaaaatc aagatagaac caataaagta
    10321 agttccgata tttgaaaaga gaaaggctgt tggaatataa agagcgaccc cgatcaagcc
    10381 ccctccagca aagctagtcg ttcgaaaacc agtcagatct gtcacaacct gagccatggt
    10441 cccttttaga acggacttgt ccaaaccata tttccaaacc aagtaggcct caaaaatcaa
    10501 gagtaagcca gcaaatatgg tgaaaaagcc agataagagt ccttcctgtt ttcgtatcca
    10561 cttgaaaaag aagagataga ttaataggcc gaatatcgcc agataagcta ggctacccac
    10621 tagcaagcga attaaattat aaagggttat acctgcagcc cctaatttga aggctgcgaa
    10681 aatcaataaa atcgcaattc ctaacgaaat caacattcgt tgaatcgctt cttttctttc
    10741 cagttctgct ttagacggtc tccgtcttgt tgtacttgta ttcttgtttg ccattcttct
    10801 attataccat atttcacagg catttcggga agagaaaaag actgcaccaa acggtccaat
    10861 cttttcattt ttctattttt tatgcttgtg gtttgcgtag gtaaccataa accacaccac
    10921 ttacgattgc tcctaccaag acagaaacga ggtaaaggag agcatttgaa gtaagggcga
    10981 taacgaagat tcctccgtgt ggcgccatga gtttgatacc agtaagacca acgagtccac
    11041 ctgctactgc tgaaccaagg atgaagcttg gaatcgcacg agctgggtca gcggcaccaa
    11101 atggaatcgc tccctcagtg ataaatgaca agcccatgat gatgtttgtc aaaccagagt
    11161 tacgttcttc cttagtaaat ttatctttga aaagaagagt tgcgacaaag attgcaagtg
    11221 gtggcaccat tcctccagcc ataactgctg ccatggctac agaaccacct gaagaaacag
    11281 ttgctgcaag cgtacctgta ccaaagacat aagctgcttt attaactggt ccacccatgt
    11341 caacagccat cattccacca aggacgatac caagaaggac agctgaacct cctccaagac
    11401 cacctaggaa gtcattcata gcagtgttga ttgcagccat tgggatattc acagctagca
    11461 taacaaatcc tgtcaagatt gttccaaaaa gtggcaatag aaggattgat ttagcacctt
    11521 cgagtgaacg aggaacttta acgtatttct tgatggcaag aaccaaggca cctgcgataa
    11581 atccaccaac aagggcacct aggaaaccag atgagacacc tgcaagagtt gaagttgctt
    11641 caccacctgc ggcataagga attttaccaa aggcaaaacc ttctttggca atagcaccag
    11701 ccacgaaacc tgctaccaaa cctggttttt cagcaataga gtaggcaaca taacccgcaa
    11761 agactggaag catcaaacca aaggcagctc caccaatttt catgaacata gaagctaact
    11821 catggtaaga accaagattg ccaaggtttt catttggaac acccaaagca ccgtcaatca
    11881 agaaggcaag ggcaatcatg ataccaccac cgataacgaa tggtaacatt tgagatacac
    11941 cactcatcaa gtgtttgtag aaggcaccac caaggctttg tttttcgtta gaggctgttg
    12001 cagctgttgc agcttttgca ccattagcgg cacggtagac ttcagtatct cctgaaagag
    12061 ccaagttaat tagctcttct gtcttacgga taccgtcagc aactggacga ttgatcaatg
    12121 gttttccatc aaatcgatcc atttcaacgg ccttgtctgc tgcaatgata atagctttag
    12181 ccttacggat atcttctgca gttagttgat ttccaacacc gctagcaccg ttggtttcga
    12241 ccttgatacc aacccccatt tcagcagcta ctttttgaag ggcttcttgg gccatgtaag
    12301 tgtgggcaat acctgttgta caagctgtaa cagctacgat aaagtcacca gagtcattag
    12361 caggtgcttg aacaagttcc tcagtttttt ctgaagcttg gtcaaaaagt tcgataactt
    12421 ggtctgcaga tgttgcttga cgaagtttgt ctgcaaaacc gtctttcatc aagtattgag
    12481 acaattctgc caaggctgcc aagtgagtat cattggcacc ttctggagct gcaatcatga
    12541 agaagaggtc agttgcttgt ccatccaagc tctcgtagtc aacaccctta tttgacttag
    12601 caaatagaac tgtcgcttct ttgacagcag cgtttttgct gtgaggcatt gcgattccat
    12661 cacccaaacc agtagaagtc aaagcttcac gcgccaaaat tccttcttta aatgtttcaa
    12721 aatctgttac ataaccgtgg tctgtcaaat ttttaatcat ctcgtcgatg acagctgttt
    12781 tttcagttgc ctgcaaatct agcaacatga catcttttct caataggtct tgaattttca
    12841 tcgtttttct acctcaactt ttccatatgt ttctttaata aattccgccg ttgccaagtc
    12901 atctgagaag gtagttgccg ttccgcaagc cactccccat ttgaaggctt ctactacgtc
    12961 ttttgatttg acaaattcac ctgtgaatcc agcaaccata gaatcaccag ctccaactga
    13021 atttttgact gttcctttga ttggtttagc gaagtaagct ccctcagatg tgacaagaag
    13081 ggcaccatca ccagccatag agataataac attttgagca cccttagcca gtaactcacg
    13141 agcgtatttc tcaatttcat ctaaactttc gagtttaacc ccaaaaatcg ctccaagttc
    13201 atgattgttt ggttttacaa gaagaggctg gtagtccaaa ctatcaatta aggtctgtcc
    13261 ttcaaagtca cagaccactt gcgcaccagt ctggcgcgtc aaggaaatca aatccttata
    13321 gataacattg cctagatttt tagcacttga acctgcaaag acaactgtat cttctgctgt
    13381 cagactagat aaaatagctt tcaattcttc tagctgaacc gattcaacag ttggacccgt
    13441 tccgttgatt tctgtttctt ggtctgcttt gattttaaca ttgatacgag tatcttctgc
    13501 cacctggaca aaacgtgtct cgatttcttc ctctgctaaa gtatctgtga taaatttacc
    13561 agtaaagcca ccgataaatc ccgtcgctgt atttggtata ttcaaacgtt tcaagacacg
    13621 gctgacattg attcctttcc caccagcaaa cttatcatca ctgtccatac gatttacact
    13681 accaactttg acttggtcca aacgaacgat atagtcaatg gatggattga gtgtgactgt
    13741 ataaatcata cttctattac ctccgttttc tccttaataa cctgcaagag ctcatgccct
    13801 tgactagtga taacgatagc gcgtttgagt ggtgctacct tggcaaagca agtttgtcca
    13861 atttttgacg aatccaccaa gacgtaggtc tgcttggcat tctccaaaat agctcttttc
    13921 acagctccct cctccatatc aggagtcgta taatagccat cgtcaacacc attcattccg
    13981 ataaaggcac ggtcaaagtg caattggtta atctggttaa gagcaacgcc cccgatacta
    14041 gcatctgtcg ccgtcttgac gtttcctcca accatgacag ttggaatctg cttttcaacc
    14101 aactgagcag catggtgaat ggagttggtc acaactgtaa cattcttatt gaccaattca
    14161 tgaatcaaaa aagcagttgt tgttccagca tcgataaaga tgacatcttt ttctttaatg
    14221 agagaggctg ctttctgagc cagcaacttc ttttcttgaa ggtttttgac agatttttct
    14281 tgaatggttt cttcttcctg taaggagtgg gggagttctg ctccaccatg cacacgacga
    14341 agcttgtttt ccgcttccaa ctcatccaag tctcttcgaa ccgttgattc tgacgtttct
    14401 agcaaactaa ctaatttttc tagagaaact acatgatgtt gatttaactc ctctaaaatc
    14461 agttgtttac gttctgtttt taacactgaa tcacctcctg ttatcgttta cactattcat
    14521 tctaccatac tttatttcga agtcaagcat tttttgtcat tttctttcaa aatctatcat
    14581 ttttcttatt tctagaattt ttattgcaag ataagagcct ttatggtaga ctatttgagt
    14641 aagtattgga agattactca agaggcttaa gaggccgtgt tggaaacgcg gtaggcgtgt
    14701 aatagcgtgc gtgggttcga atcccatgtc ttccgtcgaa aaaagattat ttttaggatg
    14761 cgacaaatgt tgtatccttt tttaaaatag ggtctgtttg tgactccctt tttagcaaaa
    14821 gaatgcaaag gaataccaaa tataaaaata aaaaacgttg atttaacaac gttttaccaa
    14881 ggaatgcaaa agaatgcaaa ggaataatgg agccggtggg agtcgaaccc acgtccaaac
    14941 acctgccaac atatttgtct acaaccatag gttatgtatt attttaacag cccctcgaca
    15001 cataactcaa gtctagaaac tgcgagtcta tcaatctctt atcaagccgc tagacaaggc
    15061 ttaatcgtat ctcgctaata ataagacctg tcattgaaca cgagcaatcc aaatcgggtc
    15121 acgcctgctg gtttttaggc agctagagcg taagaagtgt tattttttgc agttatattt
    15181 aactgagcgt ttacgtcgcc acacgagtcg caaaatatgc ctcataatgc ctgtcgaatc
    15241 cgtaacgacc ccaaaagaca ataaaactat tataccttat ctaactcaaa aatgcaaaaa
    15301 gaaatcacat acctaaaaaa gatactattc gagaaattta gcaaactgga agactaacta
    15361 caactttctc agtgcataac tttacgattg taaatagagc ttacgaaatc caattaacac
    15421 atacctaaga tgaagctatc tgttttgaag aaattcacga agagaattat tctaaaatac
    15481 tttcagatga gaattttcgt actctcttta ttttaattca tttgagttta aaaactataa
    15541 aatcaatcct tatattattc ggactacttt tca
//
LOCUS       DAVGJJ010000043        15503 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000043 DAVGJJ010000000
VERSION     DAVGJJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15503
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.043"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..364)
                     /locus_tag="VVE35_001245"
     CDS             complement(<1..364)
                     /locus_tag="VVE35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU3061431.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIET"
     regulatory      393..489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            487..996
                     /locus_tag="VVE35_001246"
     CDS             487..996
                     /locus_tag="VVE35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU3061432.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     gene            1096..1168
                     /locus_tag="VVE35_001247"
     tRNA            1096..1168
                     /locus_tag="VVE35_001247"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:Thr,seq:ggt)
     gene            1799..2875
                     /locus_tag="VVE35_001248"
     CDS             1799..2875
                     /locus_tag="VVE35_001248"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU3061433.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEKSTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRNTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            2931..3866
                     /gene="dapA"
                     /locus_tag="VVE35_001249"
     CDS             2931..3866
                     /gene="dapA"
                     /locus_tag="VVE35_001249"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU3061434.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(3913..5286)
                     /gene="mnmE"
                     /locus_tag="VVE35_001250"
     CDS             complement(3913..5286)
                     /gene="mnmE"
                     /locus_tag="VVE35_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU3061435.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(5423..5605)
                     /locus_tag="VVE35_001251"
     CDS             complement(5423..5605)
                     /locus_tag="VVE35_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU3061436.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            5728..6318
                     /locus_tag="VVE35_001252"
     CDS             5728..6318
                     /locus_tag="VVE35_001252"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU3061437.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALTIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            6315..7394
                     /gene="prfA"
                     /locus_tag="VVE35_001253"
     CDS             6315..7394
                     /gene="prfA"
                     /locus_tag="VVE35_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU3061438.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            7394..8233
                     /gene="prmC"
                     /locus_tag="VVE35_001254"
     CDS             7394..8233
                     /gene="prmC"
                     /locus_tag="VVE35_001254"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU3061439.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLATHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADISQEALDLARENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            8217..8819
                     /locus_tag="VVE35_001255"
     CDS             8217..8819
                     /locus_tag="VVE35_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU3061440.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            8821..9252
                     /locus_tag="VVE35_001256"
     CDS             8821..9252
                     /locus_tag="VVE35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061441.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            9314..10570
                     /locus_tag="VVE35_001257"
     CDS             9314..10570
                     /locus_tag="VVE35_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU3061442.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAVNVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     AENEAVLEEVRSEVKELTDAFLLYED"
     gene            10578..11552
                     /locus_tag="VVE35_001258"
     CDS             10578..11552
                     /locus_tag="VVE35_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU3061443.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLTNLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSENLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            11552..12166
                     /gene="pmp23"
                     /locus_tag="VVE35_001259"
     CDS             11552..12166
                     /gene="pmp23"
                     /locus_tag="VVE35_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU3061444.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            12240..13214
                     /locus_tag="VVE35_001260"
     CDS             12240..13214
                     /locus_tag="VVE35_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU3061445.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            13288..14919
                     /gene="rlmD"
                     /locus_tag="VVE35_001261"
     CDS             13288..14919
                     /gene="rlmD"
                     /locus_tag="VVE35_001261"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU3061446.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            15133..15411
                     /locus_tag="VVE35_001262"
     CDS             15133..15411
                     /locus_tag="VVE35_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061447.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
BASE COUNT         4767 a         2958 c         3311 g         4467 t
ORIGIN      
        1 ttgtttcaat agcatttatc ttatcaatgt tttcagttac gaattgccct ataatgtgag
       61 gaaattgggt tacaaagaga aaagttaaaa acaagtctaa cagaaaagta gaaaggcgag
      121 caagagaaaa tttcactaaa cggataagtc tatttttctt tttttgctta aatacaatcg
      181 tatcatttgt gataaaggca aataggatac cgataatatt tgctagggca gtagccagga
      241 gttcctgatg acttagttga taaatgacta gtcgtgagag aatcgaaact agagttgtcg
      301 caaggccaaa aaagagatag gctaagattt cattgttaaa aaatttttga ataggttttt
      361 tcatgcttta agtatagcat aaagcaggct attgtgctat actagtaagg ttgaatgaat
      421 caacccttgg tgcttagctt ctttcaccaa gcatattaca cgcggataac cgccaaagga
      481 gaaaagatga aaaaattaac tattcgtgat gttgcagata ttgcaatcgt cgctgctatc
      541 tacgtcgttt tgactgtcac accgccccta aatgccatca gctatggtgc ttatcagttt
      601 cgtatttcag aaatgatgaa ctttatggct ttttacaatc ctaagtacat catcggagtt
      661 accatcggtt gtatgattgc taatttcttt agcttcggac tgctagatgt ctttgttggt
      721 ggtggttcaa ccttagtatt cctcagttta ggtgtttggc tttttgcaaa atatagcaag
      781 gattacctct ttaacggatt gattcgaaaa gatcatttct tcttttcaat cctcttttca
      841 atttccatgt ttactatcgc tgcagagtta aatattgtag cagaattacc attcttcctt
      901 acttggttta caacaggaat cggtgaattt gcttcattga ttattggagc gattattatt
      961 ggtaaaattg gtcgtcgaat tgatttaagt aaatagaaga agataagcta ggtagttctt
     1021 acctggcttt ttcctatgga aaatttctaa ggaaacttga caagtttttc tgattatagt
     1081 atacttctta agtaagctga tttagctcag ttggcagagc gcatccatgg taaggatgag
     1141 gtcgccggtt caatcccggc aattagcatt aaatagacag aaaagccctt gatttacaag
     1201 ggtttttgtt atgtctgccc ccaatttttc caatatattt cttatgcacc tgtgtaaata
     1261 aaactaatac tcaatgaaaa tcaaagagta aactaggaaa ctagccgcag gttgctcaaa
     1321 gcactgcttt gaggttgtag atagaactga cgaagtcagc tcaaaatatg tttttgaggt
     1381 tgtagatgaa actgacgaag tcagctcaaa acatgttttt gaggttgtag atgaaactga
     1441 cgaagtcagt aaccatacat acggcaaggc gacgctgacg aagtttgaag agattttcga
     1501 agagtataaa gagtttcgtc tgttgacaaa agtcattctc tttagtagaa tagtagatgc
     1561 gatgagtcga taggtagtct tcgggctact attgagcata aggaggtcat aacgcaggag
     1621 cggaccttga tgagttgtgt gaacctgctc atcacatgaa gatgcctctt aatccctagt
     1681 ctagcgattg gggattttta ttttccaaaa aatgatgaaa aagctttgca attcatggaa
     1741 aaagtagtat aatacttcta ttatagaaat ttttagaaaa ttccgaaaga ggttatttat
     1801 gggatataca gttgctgtag tcggcgcgac aggtgctgtc ggtgctcaga tgataaaaat
     1861 gttggaaaaa tcaacacttc caatcgataa aatccgttac cttgcttctg cacgttcagc
     1921 aggtaagtca ttgaaattta aagatcaaga tattacgatt gaagaaacga ctgaaacagc
     1981 ttttgaagga gttgatattg ctctcttttc agcaggtggt tcgacatcag ctaagtatgc
     2041 accatacgca gtaaaagctg gcgtggtagt agtggataat acatcttatt tccgtcaaaa
     2101 tccagatgtt cctttggttg ttccagaggt caatgctcat gcacttgatg cccacaacgg
     2161 aatcattgcc tgccctaact gttcaacaat ccaaatgatg gtggctcttg agccggttcg
     2221 ccaaaaatgg ggcttggacc gtatcattgt ttcaacttat caagccgttt caggtgctgg
     2281 tatgggagca attcttgaga cacaacgtga acttcgtgag gtcttgaatg atggagtgaa
     2341 accacgtgat ttgcatgcgg aaatcttacc ttcaggcggt gacaagaaac attatcctat
     2401 cgccttcaat gctcttccac aaatcgatgt cttcactgac aatgattaca cttacgaaga
     2461 gatgaagatg accaaggaaa ctaagaaaat tatggaagat gatagcattg cagtatctgc
     2521 aacatgtgtg cgtattccag tcttgtcagc tcactctgag tctgtttata tcgaaacaaa
     2581 agaagtggct ccaatcgaag aagtaaaagc agctatcgca gccttcccag gtgctgttct
     2641 tgaagatgat gtagctcatc aaatctatcc tcaagccatc aatgcagttg gttcgcgtaa
     2701 tacctttgtt ggtcgtatcc gtaaagactt ggatgcagaa aaaggaattc acatgtgggt
     2761 tgtttcagat aaccttctca aaggtgctgc ttggaactca gttcagattg ctgaaactct
     2821 tcatgaacgt ggattggttc gtccaacagc cgaattgaaa tttgaattaa aatagtcata
     2881 tcgtttagga gttcagatga actccttctt tgaaatagag aggtgttttc atgtcttatc
     2941 aagatttaaa agaatgtaaa atcattacag cctttattac ccccttccat gaggatggtt
     3001 ccattaactt tgatgctatt ccagccttga ttgagcattt attggcccat catacggatg
     3061 gaattcttct cgcaggaacg actgctgaga gtccaacttt gacccacgat gaggagttgg
     3121 agttgtttgc ggctgtacaa aaggttgtca atggacgcgt tcctttgatt gcgggtgtag
     3181 gtactaatga tacgcgtgac tctattgagt ttgtcaaaga agtagcggaa tttggtggtt
     3241 tcgcagctgg gcttgctatt gttccttact acaacaaacc ttctcaagaa gggatgtatc
     3301 agcactttaa ggctattgca gatgcttctg acctaccaat tattatctat aacattccag
     3361 ggcgtgtagt tgtcgaattg actccagaaa ccatgcttcg cttggctgac catccaaata
     3421 ttatcggtgt caaagaatgt actagcttgg ctaatatggc ttacttgatt gagcacaagc
     3481 ctgaagagtt cttgatttat acaggtgagg atggagatgc tttccatgcc atgaaccttg
     3541 gggcggatgg ggttatttct gttgcctctc atacaaatgg ggatgaaatg cacgagatgt
     3601 ttactgccat tgcagaaagc gatatgaaga aagccgcagc aattcagcgt aaattcattc
     3661 ctaaggttaa tgctctcttc tcttatccaa gtcctgctcc agttaaggca attcttaact
     3721 atatgggatt tgaagctgga cccactcgtc tacctcttgt tccagcacca gaagaagatg
     3781 ccaaacgcat tatcaaggtt gtcgtagatg gcgactacga agcaactaag gcaactgtaa
     3841 caggggtctt aagaccagat tactaataaa gacaataaaa tccatgaccg aatgaaggat
     3901 catggatttt tcttattttc ctaaacagaa ttggctaaag agttgggtga tgagttcatc
     3961 tggagcagca tccccagtga tttctccgag gatttcccaa gtacgagtca agtcaacttg
     4021 aagcaaatca actggcatcc ccagctcaag accttgatta acggcttgta ggctttcaac
     4081 tgctttttca atcagggaaa tgtgacgggc gtttgacaag taagtagcat cttgctcgac
     4141 caagccagca ttttcaaaga agaggttgtt gattcgctct tcaatcttgt cgatgttttg
     4201 gtttttaagg actgaaatac ggataacgtc ttcaggtagt ttcgaagttt caatcgtttc
     4261 tggcaggtcg gttttattaa gtagaataat gcgattggta tcttggctaa tttcaagaag
     4321 ttgtctgtct tgcgcagtca gtggttcact ggcatttagc actagtagaa ccaagtcggc
     4381 ttccttgagg gcttttttcg aacgctcaac accgatttgt tccactatgt catctgtttc
     4441 acgaataccg gctgtatcaa tcaatttgag aggtacacca ttgatgttga cgtactcttc
     4501 gatgacatct cgtgttgtcc cagcgatatc tgttacgata gccttgtcct cacgcaagag
     4561 gttgttgaga aggcttgatt tcccaacgtt gggacgtcca atgatagccg ttgaaattcc
     4621 ttcacgaagg attttaccac gacgtgctgt cctaaggagt ttggttagta attgctcaaa
     4681 ctccattgtc ttctctcgga caacagcagt agtggcttcc tcaacatcgt catactcagg
     4741 atagtcgata ttgacctcaa cttgggcaag tgtgttaagg atttcttgac ggatattatt
     4801 gataagatca gaaagggaac catcaagttg tttgaccgcg atgttcatgg ccttgtctgt
     4861 cttggctcgg atgatatcca tcaccgcctc ggcctgtgtc aaatctacgc gaccgtttaa
     4921 aaaggcacgt ttggtaaatt caccaggttc tgccaaccga gctccttcac ggatagctag
     4981 ctggagaatc tcattggtca ccgcaatacc accgtgggtg ttaatctcga taatatcctc
     5041 acgagtgaag gtctttggag acttcatagc cccaaccata acctcgtcca tgactttacc
     5101 agtcagagga tcaataatgt gaccgtagtt gagagtatgg ctagcaacct gactcaagtc
     5161 ttttccttta aaaatctttt gcgcaatagc aaaactgtct gttccgctca ggcggacaat
     5221 accaatagcc ccttcaccta gtggagtaga gatagcagcg atggtatcaa attcacgtgt
     5281 aatcataatg tttcctttaa ttaatagcta ttttcttgga aattctccaa gtttcttttc
     5341 aaattgtaac aaatttagga tatttcttca atgaatgcta cttggagatt gaaaaagcct
     5401 aagcaattct gcttaagcta gcttatttgg tgcgcatttc cccctgtggg aaataagttc
     5461 cttctggcat gtcgttgatg atgacatgaa cagcagattg aggggctcca gtgttgcgaa
     5521 caaccgcttc cgttacttcc ttagcaagag ctttcttttg ctcgagcgtg cgtccttcaa
     5581 ataaatcgat gcgtacaaat ggcataatag cttcctccac tagttttgat ttcttccatt
     5641 ttaccacatt ttgccgttta aagcttaaga aaattatgat atactagaat gtagcaaaaa
     5701 tttagaaatg gacgtgaagc aagaaacatg gcacagttgt attatcgtta tgggaccatg
     5761 aactctggca aaacgattga gattctcaaa gtggcctata actacgagga gcaaggaaaa
     5821 ggtgttgtca ttatgacttc ggctctggat acgcgtgacg gtgttggcta tgtgtcgagt
     5881 cgaattggca tgaaacgccc tgcccttacg attgaggaaa caacggatat ctttggctat
     5941 atccgagacc taccagaaaa accttactgt gttttggtcg atgaagccca gtttctcaaa
     6001 cgccaccatg tttacgacct agctcgtgtt gtggacgagt tagacatacc cgtcatggct
     6061 tttggcttga aaaatgactt tcgcaatgaa ttgttcgaag gttccaaata tctcttgctt
     6121 ttagcagaca agattgacga aatcaagacc atatgtcagt attgcaagaa aaaggcgacc
     6181 atggtgttgc gaacacagga tggactgccc gtttacgatg gagaacagat ccagattggt
     6241 ggtaatgaaa cctatatctc ggtttgccgt aaacattatt ttgcccctga aatcaataag
     6301 gagaatgaag aaaaatgaac atctatgatc aactacaagc tgtagaagac cgttatgaag
     6361 aactaggaga attgctgagt gaccctgatg tcgtttcaga caccaagcgt tttatggagc
     6421 tttcaaaaga agaagcttcc aatcgtgaca ccgtaatagc ctaccgtgag tataaacaag
     6481 tccttcaaaa tatcgtcgat gccgaagaga tgattaagga atcaggcgga gatgcggact
     6541 tggaagaaat ggccaagcaa gaactcaaag atgccaaggc tgaaaaagaa gaatatgaag
     6601 aaaaactgaa aattttgctc cttccaaagg atccaaacga tgacaagaat atcatccttg
     6661 aaatccgtgg agcagctggt ggagacgaag cggcactttt cgctggagat ttgctaacta
     6721 tgtaccaaaa gtatgcggaa gcccaaggtt ggcgctttga agtcatggaa gcctctatga
     6781 atggtgtcgg tggttttaaa gaagtggttg ctatggtttc aggtcagtct gtatactcta
     6841 agcttaagta tgaatcaggt gcccaccgtg tgcaacgtgt tcctgtgaca gaaagccaag
     6901 gccgtgttca tacttcgaca gcgacagttc ttgttatgcc agaagttgaa gaggttgaat
     6961 acgacattga tccaaaagac cttcgtgtcg acatctatca cgcctctggt gctggtggac
     7021 agaacgtcaa taaggttgcg actgccgttc gtattgttca cttgccaacc aatatcaagg
     7081 ttgagatgca ggaagaacgt acccagcaga agaaccgcga gaaggctatg aagattatcc
     7141 gtgcacgcgt cgctgaccac tttgctcaga ttgctcagga tgaacaagac gctgagcgta
     7201 agtcgacaat cggtactggt gaccgttcag aacggatccg aacttataac ttccctcaaa
     7261 accgtgtcac agaccaccgt atcggcttga ccctccaaaa actagatacg attttgtctg
     7321 gtaaattgga cgaagttgtg gatgccttgg tgctttatga ccaaacacaa aaactagaag
     7381 aattaaacaa ataatgaaat tagctcaatt attttcaaat tttgaagaag agttgataag
     7441 acaaggagag gaagcagaaa gcctctcttt tgtctatcgt agtctgaaaa atctatcttt
     7501 tacagacttt atttttgccc tccagcagga ggtgacaaca gaggaagaaa aacaatttgt
     7561 agaagatatt taccagcaac tagcaactca caagccagct cagtacatca ttggacaggc
     7621 agatttttat ggaatgcatt taaaggtgga tgaacgggtt ttgattcctc gtccagaaac
     7681 agaggagtta gtggagctta ttctaactga aaatcttgag acgaatcttt cagttctgga
     7741 tattggaaca ggtagtggag ctattgctct cgctctagca aaaaatagac cagattggtc
     7801 agtgacagca gcagatattt ctcaagaggc cttagattta gctagagaaa atgctagcaa
     7861 tcaaaatctt aatatatttt ttaaaaaatc tgattgtttt gcagaaattt ctgaaaaata
     7921 tgatataatt gtatccaatc caccctatat ctctcgtgaa gatgagtcag aggtaggctt
     7981 gaatgttttg cattcggagc ctcatctagc tctctttgca gacgaggatg gcctagctat
     8041 ttactgtaga attgcggaag atgcaaaaga ctatctcaag gatggtggta agatttacct
     8101 tgaaattgga tacaagcaag gtcaaagtgt tcccgaactt tttagaaaac atcttcctga
     8161 aaaaaaagtt cgaacactca aagaccaatt tggtcaaaat aggatggttg tggttgatga
     8221 tggacaggat tagacaagag ttggaaaatg gtggagctgt cgttctacct acagagactg
     8281 tttatggtct ttttgccaag gccttagatg aaaaagcagt tgaccatgtt taccaactca
     8341 aacgtcgtcc tagagataag gcactcaatc tcaatatcgc ctctttcgag gatatcttgc
     8401 acttttctaa gaatcagcca acttatctac aaaaacttgt agagaccttt ttgccaggtc
     8461 ccttgaccat tattctcgaa gccaatgacc gagttcccta ttgggtaaat tctgaccttg
     8521 caactattgg atttcggatg cccagtcacc ctatcacact ggatttaatt cgagagacag
     8581 gtcccttgat tgggccgtct gccaatatct caggtcaggc aagtggtgta acctttgaac
     8641 aaattctgaa ggattttgac caagaggttc tgggtctgga agacgatgct tttctaactg
     8701 gacaggattc aactattgtg gatttgtctg gagacaaggt gaaaatctta cgccaaggcg
     8761 caattaaacg agaagatatt cttgctcggt tgccagagat ttcttttgag gaggcttgaa
     8821 atgctaagag atttgcaaga aacagatgtg aaagcgatat gtgacatcaa ccaagaggct
     8881 ttgggttata cttttagtcc agaggaaacg gctagccaac tagctagact gtctcaggat
     8941 tcccatcatt tcctacttgg ctatgaggat gcagctaatc atgtcttact tggatatgtc
     9001 cacgctgaag tttacgaatc actctattcc aaagcaggat ttaatatctt agctttagca
     9061 gtttcacctc aagcgcaagg tcaaggtatc ggtaaaagtt tactacaagg gttggaacaa
     9121 gaagccaaaa gatgtggtta tgggtttatc cgcttaaatt ctgccaatca tcgtctgggt
     9181 gctcatgcat tttatgaaaa agttggctat acttgtgata aaatgcagaa acggtttatt
     9241 cgcatctttt agtttgattt tcttattgta aaatcaaact aatggactag tcacacaata
     9301 aaggagaaga cctatgattt ttgacaaaga tgattttaaa gcatatgatg ctgatctctg
     9361 gaatgctatt gccaaagaag aagaacgcca acaaaataat atcgagttaa ttgcttcgga
     9421 aaacgtagtt tccaaggctg ttatggcagc tcaagggtct atcttgacaa ataaatatgc
     9481 cgagggttac ccaggacgcc gttattatgg tggaactgat gtagtagacg ttgtagagac
     9541 tcttgctatt gaacgcgcaa aagaaatttt cggtgctaaa tttgccaatg tccaaccaca
     9601 ttcaggaagc caagctaact gtgcggctta catgtccttg attgagccag gtgatacggt
     9661 tatgggaatg gatttggcag caggtggaca cttgacccac ggagctccag ttagcttctc
     9721 tggtcaaacc tacaactttc tttcctatag tgtggatcct gaaacggaac ttttggactt
     9781 tgatgctatc ttgaaacaag cccaagaagt caaaccaaaa ctgattgtag ctggtgcttc
     9841 agcatattct caaattatcg atttttcaaa attccgtgaa atcgcagatg ctgtcggtgc
     9901 gaagctcatg gtggacatgg cccatatcgc tggtttggtt gcggctggcc ttcatccaag
     9961 cccagttcca tacgctcata tcacaacaac aacgacccac aaaacccttc gtggacctcg
    10021 tggtggtttg attttgacca atgacgaaga acttgctaaa aaaatcaatt cagctatttt
    10081 cccaggtatt cagggcggtc ctttagagca tgttgtggcg gctaaggcag tttccttcaa
    10141 agaagttttg gatccagcct tcaaggaata tgctgtcaat gtaattaaga acagcaaggc
    10201 tatggcagat gtcttcttgc aagaccctga tttccgtatt atttcaggtg gaactgaaaa
    10261 ccatctcttc cttgttgatg tgactaaagt tgtagaaaac ggcaaagttg ctcaaaactt
    10321 gttggatgaa gtcaatatta ccttaaataa aaactcaatc ccttacgaaa gcttgtcacc
    10381 attcaagaca agtgggattc gtatcggagc agcagccatt actgcacgtg gatttggtga
    10441 agaagaaagt cgcaaagtgg ctgaactcat cattaaaacc cttaaaaatg cagaaaatga
    10501 ggctgtctta gaagaagtga gaagtgaagt taaagaattg acagatgcct tcctactata
    10561 cgaggactaa aacttttatg gacatttata ttaagaaagc cattattcac cagttcagtc
    10621 cggatgatac cgaactgttc ttagcagata agtttctcaa tattactcca aaaatcgaag
    10681 aatacctgcg taaaaaaatt gaacatgtgt attcagatga agccaagact gggattttcg
    10741 aagaagaaaa tcccttcttc aatcatatta cagacgattt gttggagaca tcagtaacac
    10801 tgactaatct ctggaaagag gagttcagta tttctgaaaa tctaaaaact aatgacttga
    10861 tttttgtgca attttctaaa gaaggtgtag aacatttcgc tttcttgcga attgccctgc
    10921 gtgagacctt gacccatctc ggtggagaag ttgataatcc aatcaagctg actcagaata
    10981 acctgcctgg atttggaacg ggtgctgacg aggccttggt ggtcaatctt cagagtcgca
    11041 agtaccatct gattgaaaaa cgaatcaagt acaacgggac ttttttgaac tatttttcag
    11101 aaaatctcct agctgttgcc cctaagattt cacctaagaa atccatcaag gaactggaaa
    11161 aaacagccca gagaattgcc gaatctttta acacagatga ttttcaattt caatccaagg
    11221 tcaaatcagc tatttttaac aacctagaag aaagcaatga attgtcacct gagaaattgg
    11281 ctaatgacct ttttgacaac aatctgacgg ctcgtttgag ctttattgac caagtcaaag
    11341 aagccgtacc agaacctgtt caatttgatg aaattgatgc cagtcgccaa ttaaagaaat
    11401 ttgaaaacca aaaactctcc ttatcaaatg gaattgagct catcgttccc aataacgtct
    11461 atcaagacgc cgagtctgtt gagtttatcc aaaacgaaaa tggaacctac tctatcttaa
    11521 tcaaaaatat cgaggatatc caaagtaaat aatgtttaaa cgaattcgaa gagtgcttgt
    11581 actagcagtc ttcctttttg ctggctataa agcttaccgc gttcatcaag atgtcaaaca
    11641 agtcatgacc tatcaaccca tggtgcgaga aatcttgagt gaacaagaca ccccagcaaa
    11701 cgaagagctt gtgcttgcta tgatttatac tgaaacaaaa ggaaaagaag gcgatgttat
    11761 gcagtctagt gagtctgcaa gtggttccac caacaccatc aatgataatg cctctagcat
    11821 tcggcaaggc attcaaactc tgacaggcaa tctctatctg gcgcagaaga agggtgtaga
    11881 tatctggaca gctgttcaag cctataattt tggacctgcc tatatcgatt ttatcgccca
    11941 aaatggcaag gaaaatacct tggctctagc caaacagtac tctcgtgaga ctgttgcccc
    12001 cttgcttggt aatacgactg gaaagactta tagttatatt catcccattt ccatttttca
    12061 cggtgctgaa ctctatgtaa atggaggaaa ctattattat tctagacagg tacgacttaa
    12121 cctttacatc atcaaatgtt tcactctctt ttcaacatct ggctagacca ggtgtttttg
    12181 ttataagttt tctttaagat agatatatta ctctaaagaa gtaaaggagg aaattcccta
    12241 tgagaaagaa actctttctg actagcgctg cgatcttgtg ggcagtaaca gctatgaata
    12301 gcgtccatgc agcaacagat gttcaaaaag ttatcgatga aacctatgtc caacctgaat
    12361 atgtcctagg ttcttcccta tctgaagacc aaaaaaatca aactcttaaa aaactgggct
    12421 acaatgcctc aacagatacc aaagaactca agaccatgac acctgatgtt tattctaaaa
    12481 tcatgaatgt ggccaatgac tctagcttac agttgtattc atcagccaag attcaaaagc
    12541 taggtgacaa atcgccactt gaggtcaaga ttgaaacacc tgaaaacatc actaaggtaa
    12601 ctcaggatat gtaccgaaat gcagccgtaa ctttgggtgt ggaacacgcc aaaatcactg
    12661 tagcagcacc cattccagtt acaggtgaga gtgctttggc tgggatctac tattcgctag
    12721 aagctaatgg agccaaggtg ccgcaagcca ataaagattt ggctcaagaa gagctaaagg
    12781 ctttgtcaga tatcaatgct gaaaacaagg acaaatcagg ctatgatgct aataaattaa
    12841 acgttgccct agctgatatc aagtcaggac tcgccaaagc taaagaaagc aagggaaatc
    12901 tgacagaaga agatatccgc aagattgttg aagatacctt aaaaaattac aaacttgatc
    12961 aggtcataac aggaaaccag atcaatatca tcatcaattt tgccttgaat ctctcaaaga
    13021 gtgatatcct cagcaatgca gatttcacta aaaccttaaa tgaccttaaa caaagcatcg
    13081 tatcacaagc tggcgacagt tttaaaaata tcaaccttaa ctttgatgcg gataaggcgc
    13141 tagaggacgg tggtaacttc ttaagctccc tctggcaagc ccttgtcaac ttcttcaaga
    13201 gttttggttc ttaagaaatt tcatggtata atggatggta accgtaacgt tgccgtcttt
    13261 tttgtcggcc aatagaaaga gaaaagaatg ttaaagaaaa atgatattgt agaagttgaa
    13321 attgttgatt tgacccatga aggggcagga gttgccaagg tagatggttt ggtctttttt
    13381 gtagagaatg ctttaccgag tgaaaaaatt ctcatgcgtg tcctcaaggt caataaaaag
    13441 attggctttg gaaaagttga aaaatacctt gtccagtcac cacaccgtaa tcaagatcta
    13501 gatttggctt acctgcgttc aggaatcgcg gatttaggac acctttctta tccagaacag
    13561 ctcaagttta aaaccaagca agtcaaggac agtctctaca agattgctgg aattgcagat
    13621 gtagaagttg ctgaaacgct tggtatggaa catccagtca agtatcgcaa taaggcgcag
    13681 gtgcccgttc gtcgagtgaa tggtgtcttg gaaacaggat ttttccgtaa gaattcgcac
    13741 aacctcatgc cccttgaaga tttctttatc caggatcctg tcattgacca agtcgtagta
    13801 gctcttcgag acctgctccg tcgttttgat ttaaaacctt atgacgaaaa ggaacagtct
    13861 ggattgattc ggaatcttgt ggtgcgtcgt ggtcactatt caggacaaat catggtcgtt
    13921 ttggtgacaa ctcgtccaaa agtttttcgt gttgaccaat tgattgaaca agttatcaag
    13981 cagttcccag agattgtgtc tgtcatgcaa aatatcaacg accagaatac caatgcgatt
    14041 tttggtaagg agtggcgcac tctttatggt caagactata ttacggacca gatgttggga
    14101 aatgacttcc aaatcgctgg cccagccttt taccaagtca atactgaaat ggcggagaaa
    14161 ctctatcaaa cagccattga ctttgcagag ttaaaaaaag atgatgtggt tattgatgct
    14221 tattctggta ttggaaccat tggtttatca gtcgccaagc atgtcaaaga agtctacggt
    14281 gttgaactga ttccagaagc ggttgagaat agtaaaaaaa atgctcagct gaacaatatt
    14341 tcaaacgccc actatgtctg tgacacagct gaaaatgcta tgaagaattg gcttaaagat
    14401 gggattcaac caaccgttat cttggttgat cctccacgca agggcttgac agaaagcttt
    14461 atcaaagcaa gcgcccaaac aggagccgat cgcatcgcct atatctcctg caatgtcgca
    14521 accatggcgc gtgatattaa actataccaa gagttgggat atgaattgaa gaaagtccag
    14581 ccggtggatc tatttcctca aacgcatcac gtcgagacgg tagcactttt gtccaaactc
    14641 gatgtcgata agcacataag tgttgaaatt gagctggatg agatggattt gacaagtgcg
    14701 gagagcaaag caacatatgc tcaaatcaaa gaatatgttt ggaataaatt tgaattaaaa
    14761 gtttcgacat tatatattgc acagataaaa aagaaatgtg gaatagaatt acgagaacat
    14821 tacaacaagt ctaaaaagga taaacaaatt attccacagt gtacacctga aaaagaagaa
    14881 gccatcatgg atgctttgag acacttcaaa atgatttaat agaaaagaat gacagtatat
    14941 gactttctgc atttattaca ttcctacttg gtataggaac agctattatt cctttcttgc
    15001 aaggtatcaa ttagaaaata ggctcaatat aaagattgat aggatcattt ttatatttaa
    15061 aggagcgttg aaatgattga taaaggcaac aaaaaatttt aggataaatt tgctaagttg
    15121 tatgcccctt ttatgaaaaa agataaagag gtttatgata aagtttgtga atatcttagt
    15181 cctcatttga ataaagatat ggaggtgctt gaacttgctt gttggtttcg tgtcataaca
    15241 gttatagagg caaatagtta tgtaaatata aggagttcaa gacttctacc aaagtttaaa
    15301 actcaaaaaa taaatagttg gtgtgctgct tacaatatcc attttaataa tggatattgt
    15361 aagcagcata ccaatgaatt taaagattct ttaaagagtc ttattttgtg atgaaaattt
    15421 aatatgtaaa tctcagacga tagaaattaa aaactctatc gtctttttta tactcaaaat
    15481 taggaggtaa aaatggtaag gat
//
LOCUS       DAVGJJ010000044        15355 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000044 DAVGJJ010000000
VERSION     DAVGJJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.044"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1451
                     /locus_tag="VVE35_001263"
     CDS             <1..1451
                     /locus_tag="VVE35_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU3061448.1"
                     /translation="FFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDFVLQ
                     NLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQEIDY
                     ITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLITHLM
                     TLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVSLHF
                     LAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQEKLK
                     GIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINKFEN
                     NSFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSSVVF
                     SEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTK
                     KIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            1461..1775
                     /locus_tag="VVE35_001264"
     CDS             1461..1775
                     /locus_tag="VVE35_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU3061449.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            1806..2312
                     /locus_tag="VVE35_001265"
     CDS             1806..2312
                     /locus_tag="VVE35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061450.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKVYQQFVQ
                     LEKNSLSL"
     gene            2392..3738
                     /gene="celB"
                     /locus_tag="VVE35_001266"
     CDS             2392..3738
                     /gene="celB"
                     /locus_tag="VVE35_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU3061451.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            4118..4294
                     /locus_tag="VVE35_001267"
     CDS             4118..4294
                     /locus_tag="VVE35_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061452.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            4695..6908
                     /locus_tag="VVE35_001268"
     CDS             4695..6908
                     /locus_tag="VVE35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEU3061453.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSNIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(7130..7606)
                     /locus_tag="VVE35_001269"
     CDS             complement(7130..7606)
                     /locus_tag="VVE35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU3061454.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLISRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            7829..11032
                     /locus_tag="VVE35_001270"
     CDS             7829..11032
                     /locus_tag="VVE35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU3061455.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRIYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKYVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            11416..11792
                     /gene="tnpA"
                     /locus_tag="VVE35_001271"
                     /pseudo
     CDS             11416..11792
                     /gene="tnpA"
                     /locus_tag="VVE35_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12180..12809)
                     /locus_tag="VVE35_001272"
     CDS             complement(12180..12809)
                     /locus_tag="VVE35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU3061456.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(12819..13820)
                     /locus_tag="VVE35_001273"
     CDS             complement(12819..13820)
                     /locus_tag="VVE35_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU3061457.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(13851..14492)
                     /locus_tag="VVE35_001274"
     CDS             complement(13851..14492)
                     /locus_tag="VVE35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU3061458.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(14511..15326)
                     /locus_tag="VVE35_001275"
     CDS             complement(14511..15326)
                     /locus_tag="VVE35_001275"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU3061459.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         4898 a         2530 c         3207 g         4720 t
ORIGIN      
        1 aattttttaa aacaatacat tgctatgtaa aatttaactt ttttgatcaa actgttccat
       61 tagaagaatt tgctagaatt gttttggatg aatgccaaga ggccaaccta aaactatctg
      121 attttgtact acagaattta gtagttcaca tagcattgtc tgtgattcgt ttaaaatccg
      181 gatttgaaat aaaaaatata gactgtcaga tgactgatga tgcaatagag agaaaggttg
      241 cccaaagaat tctttctaaa gtaagagaag taacaaatca ggaatttcca gttcaggaaa
      301 tagactacat tactttgcat ttgttagcta agagtcaaca atgtcaaaaa aatcagaaga
      361 atatttctga agaagtttta aaaaaatctt tatttaaaac atttcagaat ttaggattag
      421 atgatatgta taacttttct tcagatttcc aactaattga aggattgata actcatctaa
      481 tgacactaca agtacgttta gaaagccgga ttaccttaaa taaccctttg gtagatgaaa
      541 ttaaacagaa ttatagtgat atattcttta tgactagaga aattcttgca aatatggaca
      601 agttcttgga gtggacaata tcagatgatg aagtagcata tgtaagtctt cactttttag
      661 ctgctatgga acgtagcaaa gagagtacta agtttaatat tcttgctatt tgtgcaacag
      721 gatttggtgc agcccaaatg ttaagaaatc gtttggaaac ggagtttggg aagcgagtag
      781 aagttgttga tgtaataggc tattatgaat tgaatcaaga aaaacttaaa ggaatagatt
      841 ttatagtgtc agctgttgat ttatcaaatc tatattttca aatcccagtt tttaaagtta
      901 gtgtgtttct taagagtgat gagatggaaa tgattagaaa agctatggat cagatgcaag
      961 tttctagtta tgtacaatca tcaaagatta acaaatttga aaacaacagc tttagacagt
     1021 attttagtaa agaaaatttt ttaatatgta ctgaatcaga caaggtaaat ttacttgaaa
     1081 agatggttga gggtttatct gttggtgaat caaatgagtt tgaacaatct ttactttatg
     1141 gaattaaaca gcgcgaagaa ttaagttcag ttgttttttc tgaaaaaatt gcagttccgc
     1201 atcctattca gccctttggt acagagggaa aggtttcggt tgcaatttgt aaagattcgt
     1261 tactctggga taatcaaagt tcacatgtcc agctagtatt tcttctatct ccatcaatat
     1321 atgggaacga aggtttggca actgtgacta aaaagatagt ctctctgaca gagaatgatg
     1381 aattgcaaaa tcagctaata tcttgcaata actttgaaga ttttattaac atatttgaga
     1441 agataaaata ggaggttcat atgaatcaag aggaactgca ggttgcagca tttgaaatta
     1501 tcctgcattc gggaaatgca aggtcagaaa tacatgaagc atttgcaaaa atgagagaag
     1561 gtagttttga tgatgcagaa agtaaattaa atcaatcaaa tgagataatt cttgaagcgc
     1621 atcatgcaca aaccaaactc ttgcaagaat acgcaagtgg agtggaaatt aaaattgaaa
     1681 ttatcatggt acatgctcaa gatcatttaa tgaccactat gacattatta gaagtagcaa
     1741 aggaaatgtt agctctttat aagaaagtta actaaatatg tgttgttata taaggaggaa
     1801 atgatatgga taaaaaagaa cgacaaaaaa ttgaacaaca acgtcgagag atggctctaa
     1861 ctaatacttt ttttaatcga tatcttcttc tacgttattc aatagcactc ttcttttttg
     1921 ggaatattta ttggcttttg agtcagttta ttagtccctc gcctattatt attttcccca
     1981 ttatgctcat agtattttct attttagcaa ctgttgaaca atttaaactc tatggaaata
     2041 ggaaagaaaa gttgggaata acactaatgt ttgttagaat acaaatgctt atttcaatag
     2101 gattacttgt tttaacttgg acaagttggt ttaagaatct ttttccgatt tttgaaaata
     2161 accaagtagc acgtttattt gtttttgtag ttcttctatt aggtttagtt ctgagtttgt
     2221 tagacattag aagaattaaa aaaatttata aacgaacaga taaggtttat caacaatttg
     2281 tccaattgga aaagaactca cttagtttat aatcctgatc tagtataatt tgtatttgaa
     2341 actagtcttt agaaagtaaa aattattatg ttttattaag gaggtctatt tatggatcaa
     2401 caaaacgggt tgtttggttt tcttgaaaac catgttatgg gaccaatggg caaacttgct
     2461 cagtttaaag tagtacgtgc tatcacggct gcaggtatgg ctgctgtacc atttactatt
     2521 gtaggatcaa tgtttttggt attcagtatt ttgccacaag ctttctcatt ttggccaatt
     2581 gtggcagata ttttctctgc ttcatttgat aaattcacat cactttacat ggttgcaaac
     2641 tatgcgacta tgggttctct atctctttat ttcgttctat cacttgcata tgaattgaca
     2701 aaaatttatg cagaggaaga agaactcaat atgaatcctc ttaatggtgc cttgcttgcc
     2761 ttgatggctt ttgtcatgac agtaccgcaa atcatttttg atggtggaat gatgaagact
     2821 gtgacaagtc taaaagaagg tgcagtaatt gcagatggat gggcaatggg aaatggagtc
     2881 gcacgttttg ggacaacagg gatttttacc gcaatcatta tggcaattgt gactgttctt
     2941 atttatcgta tgtgtgttaa acataattgg gttattaaaa tgcctgaagc tgttccagaa
     3001 ggagtttctc gtggatttac cgctttggtt ccgggatttg ttgttgcatt tgttgttatc
     3061 tttatcaacg gtcttcttgt agcaatggga acagatattt ttaaagtcat tgcaattcca
     3121 tttggttttg tatccaatct gactaattcg tggattggtt taatgattat ttatctattg
     3181 actcaactac tttggattgt aggtatccac ggtgcgaaca ttgtttttgc atttgttagt
     3241 ccaattgctc ttgctaacat ggctgaaaat gctgctggcg ggcacttcgc tgttgcaggt
     3301 gaattttcta atatgtttgt aattgcaggt ggttctggtg caactttagg actatgttta
     3361 tatattgctt ttgcctctaa atctgaacag cttaaagcaa taggacgagc atctgtagtt
     3421 ccagccttat ttaatattaa tgaaccatta atttttggat tacctattat ctataatcca
     3481 gccttggcta taccatttat tttagcacca atggttactg ctactattta ttacgtagcg
     3541 aattctctaa actttattaa gccaattatc gcacaggttc catggccaac tccagtaggg
     3601 attggagctt tcttagggac agcagatctt cgagctgtat tagttgctct agtatgtgca
     3661 tttgcagcat tcctagtcta tcttccattc atccgtgtat atgatcaaaa attggtgaaa
     3721 gaagagcaag gtatctaata agtaaaaaag tatactcgat gatagaatcc atatagttta
     3781 aataatgagt gttaatatta gcagtgaaga ggtcttttta agatattctt ctctgctttt
     3841 tcatattttt tcgctatgta gggtatgaag caaaatagaa agtagttcga atagttgata
     3901 aacagggaac tgctgttatt ttaaaaaaac aataaagtaa agtatgttat cgtagaatac
     3961 gatacaatag taaaaaagca aaaagaatgc gttttatatt tgggtttgag gcttataaaa
     4021 taggataagc gtgctgaaaa cttagatatg acaaacaaaa taaaattttt aaaagctaca
     4081 gagaaacatg gtataatgta tctaaaggag gtagcatatg tccttagata tagataaaga
     4141 aaagatgaca atcatgggaa tagcttttga aaacagatcc gtttttaaga gtgtttggta
     4201 tgctttaagt acaaatatga ttgaaggatg gcgacctacg gttagtgatg ttgaaaaatt
     4261 acgagatgaa gctcttgctc tggggatgac ttaatgaagc gcaactgtta tgagtcttat
     4321 gactatgttg atccggataa tttatatacc gatccgggtt cttcagttct aagaaataag
     4381 caggaaggaa aaaagcacgc gagcttgaat atcgtatgat tgctagtaag agtttgaaat
     4441 tatttatcaa tcctatctta gttaatagtt taaaagtagt ttgtttcaca actgcataag
     4501 cttagaaaag ttgaaaagtg ataactcttt aataaactct tggtttgtta aaagatattt
     4561 tgtccactac attttttcaa ttgactatta tttgtcatag atataacaga tagaacattg
     4621 cattcagaat aatgaaaaac gcatcaaaaa tggtgcgttc tgtttttatg ctaccataaa
     4681 aggaggaaaa caaattgaaa atttttaagg gagagtttta tcgaatctct gtattaacag
     4741 acaagctagt aaggttagaa tactctcaaa ctggaagttt tgaggataga acgacacaac
     4801 ttatctataa tagagatttt ggccaagttt cgttagatta tatcgagaca tcaaacgtac
     4861 tagatattat gacggactat tttcatctgc actttaataa aggagaattt aacgccgaaa
     4921 atttatttat agaattaaaa ggaaattttg ccgtatatgg tagtcgctgg tattttggtg
     4981 aatctattga aacgttaaaa ggaacagctc ggactctgga taaggcagat ggagcaatct
     5041 cgttagaaga tggaattatt agccgaaatg gtatagcctt attggatgat tctcaaggat
     5101 ttatttggga tgaacaatct ggttatattg agagagaaaa tcaaattgac ctgtatttct
     5161 ttgcctatgg gcatgattat agaggagcaa tcagagactt ttaccatttg actggttcaa
     5221 cacccttgtt gccaagatat gctttaggca attggtggag tagatattgg ccttatacgt
     5281 cggatgaata cttggattta atagacagat ttgaaacaga gaaaattcca ttatctatcg
     5341 gtgtgttaga tatggattgg catataactg acattccagc tcgttttgga agtggctgga
     5401 caggatatag ttggaataga aacttaatac caaatccaga acagttattg caacaacttc
     5461 atgatagaaa gttaaaactc tccttaaatg tccatcctgc tgatgggata cgggcttatg
     5521 aagaagctta tcctcaaatc gcaaaacggt tggggttaaa tgtagaacta gaagaacctg
     5581 ctatttttga tttttttaat ccctctttta gggaagccta ctttaaagat gttcattatg
     5641 agctagaaaa gcagggagta gatttttggt ggattgactg gcaacaaggg acacaaggta
     5701 tgctagatcc actttggctt ttaaaccatt atcactatca ggatagttgt aaaaatgcag
     5761 aaggtggttt gattttatca agatatgcag gtcctggtag ccaccgctac cctgttggtt
     5821 tttcagggga tactattatt agttggaatt ccttaagatt tcaaccctat tttacagcga
     5881 cagcatctaa tatcggttat agttggtgga gtcatgatat cggtggacat atgttggggg
     5941 attatgacga agagctacaa actagatggc tacagtttgg tgtttttagt ccgattactc
     6001 gattacatag ttctagaagt ccttttaata gtaaagaacc ttggtttttt tcagaaacaa
     6061 catctaatat tatgaagaaa taccttcgtt tgagacatca gatgattcct tatctatata
     6121 ccatgaatgt aaagacacat gaggaaggtg ccccattaat cagtccaatc tattatttct
     6181 atccagagaa tgatgagagc tataatgttc caaaccaata cttttttgga acagaactga
     6241 tggtggctcc cattgtagaa aagatggatt tgacattcca atctgcaaaa gtagatgtat
     6301 ggttccctga aggtgaatgg tatgacttct tttcagagaa aaaatacaca ggtggtgtga
     6361 agttaagtgt ttatagggac atctcgacta cgcctgtgtt tgcaaaaagt ggtgcaatca
     6421 ttcccttggt tggttctgag ataggtatgg gtgttgattt acctgaggtt gtagattggt
     6481 atgtattccc agggaaacaa cattcttttg aaatgcttga agatcaaaat ggtcaaagat
     6541 ataaaacaag attatcaatc gattgggaaa tgggaatggt agagttagca ttacaaggag
     6601 attctagtat cgttccaagc aatagaaaac atagaattca ttttaaagga acgaatgtgt
     6661 ctataattga attgccaaat aagaatgata cagctaaatt tgaatggaaa gataataaaa
     6721 ggacatcttt aaatgacgaa gtttttagac tactaaagac agcttctctt ccatatgaat
     6781 taaaagatag attgttaaat caattcatca atgcgaaaaa ctctcatgac ttaatgaata
     6841 tcttgcatca tcaggataag gaattgagag ggcgtttgtt ggaaatgata tttactagcc
     6901 aaaactaatt gatataatta gaaagtcatt tgtttagtgt atatgaagat tctgtatggt
     6961 attcgttatc actggtcaca ttgaaaatgt gaaataagca cctacaacat agactttaca
     7021 acacttcatg tttcatatca tggggtgttt ttctatttaa gtcaaagcca atcaaaaaat
     7081 ctcactattc atcaggttaa aggaaaccta atcatagtga gattgtgaat tatagtagag
     7141 tttgtatcgc ctcttcaatt tgttttgggt ctgtttttga agaaaagcgt tcaaagactt
     7201 gcccatcacg actaatgaga aacttagcga aattccattc gattcgtttt cctagtgggc
     7261 cagatttatg gtcttttaac cagacataga gagggtctgc ttccttaccg ttgaccttaa
     7321 tcttggcaaa acgtgggaag gtggtttgaa aatgtaggct acagaaggcg ttgatttcct
     7381 ctgcgctgcc gggtgcttgt cccataaact gattgcaagg gaaatccaat atttcgaagc
     7441 cctgatcttg atagcgttca tagagttctt gaagtccctg gtactggggc gttaaaccac
     7501 atccagtagc agtgttgaca atcaagagaa ctttgccacg atagctatcc aagggagttg
     7561 cttggttgtt ttggttcaaa acggaaaaat cataaagtga agtcatgagg gcctcttttc
     7621 tttttctaca ttgtaacagt ttttactttt ttcgtcgagt cggatggaag atattgttga
     7681 agaataagcc ctcaatatga tagaaaccgt ttacaaataa ctttaaaaat ggtatgattt
     7741 aattaaaccg gtttactttg ttgctataaa tctctaattt gagatttagt tcctattgat
     7801 tttacaatat gtttattgga gtgtatacat gcaaacaaaa acaaagaagc tcattgtgag
     7861 tttgtcttca cttgttttat caggattttt attaaaccat tatatgacag ttggagcgga
     7921 agaaacgact acgaatacca ttcagcaaag ccagaaggaa gttcagtatc agcaaaggga
     7981 tacaaaaaat ttagttgaaa atggtgattt tggtcagacg gaggacggaa gcagtccgtg
     8041 gacaggaagc aaagctcagg ggtggtcagc ttgggtagac cagaagaata gttctgcaga
     8101 tgcctcaact cgagtcattg aggctaagga tggggctatc actatctcaa gccctgagaa
     8161 attaagggca gcggttcacc gtatggttcc tattgaagct aagaaaaagt ataaactgcg
     8221 tttcaagatt aaaacagatc ataaagtcgg gattgccaaa gttcgtatca ttgaggaaag
     8281 tggtaaggac aagcgattgt ggaattctgc aacgacgtca ggaacaaagg actggcagac
     8341 cattgaagca gactatagcc cgactttaga tgttgataaa atcaagctgg agttattcta
     8401 tgaaacagga actgggactg tttcctttaa ggatattgag ctggtagagg tagcagacca
     8461 gccttctgag gattctcaaa cagataaaca gcttgaggaa aagattgatt taccaattgg
     8521 aaaaaaacat gttttttctc ttgcggacta tacttataag gtagaaaatc ctgacgttgc
     8581 ttcagtcaaa aatggaattt tagaacctct taaggaaggg acaaccaatg tcattgtcag
     8641 taaagatggc aaggaagtga aaaagattcc tttgaagatt ctagcctctg ttaaggatgc
     8701 atacacagac cgtttggatg actggaatgg catcatcgct gggaatcaat actatgattc
     8761 taaaaatgaa cagatggcca aattaaacca ggaattggaa ggaaaggtag ctgatagcct
     8821 atccagtatt tcaagtcagg cggaccgcat ctatttgtgg gaaaaatttt caaattataa
     8881 aacgtctgca aatctgactg ccacttatcg gaaattggag gagatggcca agcaagtgac
     8941 caatccttct tctcgttatt atcaagatga aactgtcgtt cgaacagtca gggattccat
     9001 ggaatggatg cataaatatg tctacaatag tgaaaagagc attgttggga actggtggga
     9061 ttatgaaatc ggtacacctc gtgccatcaa caataccttg tctctgatga aagaatactt
     9121 ctctgatgag gaaattaaaa aatatacaga tgtgattgaa aaatttgtac cagatcccga
     9181 acatttccga aagacgactg ataacccatt caaggctcta ggtggaaact tagttgatat
     9241 gggaagggta aaagtaatag ctggtttact gcgtaaggat gatcaagaaa tttcttctac
     9301 cattcgctcg attgagcaag tgttcaagtt ggtagaccaa ggtgaaggtt tttatcaaga
     9361 tggatcctat atcgaccaca ccaatgttgc ctatacgggt gcttatggga atgttttgat
     9421 tgatggcctg tctcaactgt tgccagtcat tcaaaagacc aagaatccaa tcgataaaga
     9481 taaaatgcaa accatgtacc actggattga taaatcgttt gctcctttgc tggtgaatgg
     9541 agagctgatg gatatgagtc gtggacgctc gatcagtcgt gcaaatagcg aggggcacgt
     9601 ggccgcagta gaagtactaa gagggattca ccgaatagcg gatatgtctg aaggagaaac
     9661 caaacaacgt ttgcagagtc ttgtgaagac cattgttcaa tcggatagtt attatgatgt
     9721 ctttaagaat ttgaagactt ataaggatat cagtttgatg caatccttgt taagtgatgc
     9781 aggagtcgca agtgttccaa gaacaagtta cctatctgcc tttaacaaga tggataaaac
     9841 agccatgtac aatgcagaga aagggtttgg atttggcttg tcactctttt ccagtcgtac
     9901 cttgaattac gaacacatga acaaggaaaa taaacgtggt tggtatacga gtgatgggat
     9961 gttctatctt tacaatggcg atttgagtca ctatagcgat ggctactggc caacagttaa
    10021 tccatataag atgcctggta caacagagac ggatgctaag agagcggata gcgatacagg
    10081 taaagtttta ccgtctgctt tcgttggaac gagcaaacta gatgatgcca atgcgacagc
    10141 aaccatggat ttcaccaact ggaatcaaac attgactgct cataagagct ggtttatgct
    10201 aaaggataag atcgcctttt taggaagcaa tatccaaaac acttcaacag atactgctgc
    10261 aactacaatt gaccagagaa aactggaatc aagtaatcca tataaagtct atgtcaatga
    10321 taaagaagcc tcccttacag aacaagaaaa ggattatcct gaaacccaaa gtgtcttttt
    10381 agaatcgtcc gattcgaaaa agaatattgg ttactttttc tttaagaaga gttcaatcag
    10441 tatgagtaag gctttgcaaa agggagcctg gaaggatatc aatgaaggac agtcagacaa
    10501 ggaagttgaa aatgaatttc ttacgattag tcaggctcat aagcaaaatg gagattctta
    10561 tggctatatg ctcatcccta acgtggatcg tgccaccttc aatcaaatga taaaagagtt
    10621 agaaagtagc ctcatcgaaa ataacgaaac ccttcagtct gtttatgatg ctaaacaagg
    10681 agtttggggc attgtgaaat atgatgattc tgtctctact atttccaacc aattccaagt
    10741 tttgaaacgt ggagtctata ccattcgaaa agaaggggat gaatataaga ttgcctacta
    10801 taatcctgaa acccaggaat cagctccaga tcaggaagtc tttaaaaagc tagagcaagc
    10861 agctcagcca caagtacaga attcaaaaga aaaggaaaaa tctgaagagg aaaagaacca
    10921 ttcggatcaa aagaatctcc ctcagacagg agaaggtcag tcaatcttgg caagtctagg
    10981 gttcttgcta cttggggcat tttatctatt ccgtagagga aagaacaact aatttgttca
    11041 tcatctagat gaattacatg aaactgtgga aaatagaaaa aatccgaaga aggccaaata
    11101 tcttcggatt tttcttttaa aaaaggataa gttgatactt atcctttttt acttcattat
    11161 tatgtgacta tagcattttg aaactagaat aggacgttat aactactaaa agatttctat
    11221 aaattcattt gattttccta atcaatttgt tcgtatccta tttcactcca ctatattagt
    11281 ccgtctcgct ccgtgaggtg cgagacaaaa caaccacccg ctatgcgggt gcgcgtcgaa
    11341 ggttatacca aaaaaactcc aaacgcgata caataaaggt gttcaatcca attgtaaagc
    11401 gaaaggagaa aaaatatggc acaaaaggct catagtttat catacacaaa gtggatgtgt
    11461 tctatcatat tgtgttcacc cctaagtata gacgaaaagt catctataat caatatagaa
    11521 gtagtttagg cgaaatattt catcgcttgt gtagttataa aggtgttgaa atgatcgagg
    11581 gtcacttaat gccagaccat gtacacatgt tagtcagtat tccgtcgagg ctaagtgttt
    11641 caagttttat ggggtattta aaaggcaaaa gtgcactcat gatgtttgac aaacacgcca
    11701 atctcaagta caagtttggg aatcggcatt tctgggcgga aggttattat gtaagtccag
    11761 tagggcttaa tgaagccaca attaagaaat atagtcaaga ttaaagaaat tatcgagtgg
    11821 atgatttctt cacgagtatg aaaatttgag aacgagtaaa gcatgatata tagtaaaatg
    11881 aaataagaac aggacaaaat cgatcaggac agtcaaatcg atttttaaca atgttttaga
    11941 agtagaggtg tactattcca gtttcaatct actatagcac tagataaatt gagtgtaaaa
    12001 gaatatgagg atccctttag ggatagtggt aagtaatacc aaagtctctt aaagaggcaa
    12061 gtgacgagtc aagagcaata aggcttgaac aacgtgaaag ccagcgtctt taggcgctgg
    12121 ctgatgattt gggcttatag ctctgagata aaccacccgt tagacgggta gttatgattt
    12181 tatctgagtg taatatactg ttgggcaatc tcgctgatgc ggtcaaagtt gccttgggaa
    12241 gcgagtttat tgagttcgcc accaattcca acggcatctg caccagcagc gaaccattga
    12301 gggatgttgt ttaggccgac tcctccggtt accattacgg aaacttgtgg gatcggtgcc
    12361 ttgactgcag agatatatgc tggactgaga gtactacctg ggaagagttt gatgatttca
    12421 ctaccggctt caagtgcagt cgtgatctct gtgagggtaa tacagcctgg aatgtacggt
    12481 gtgctgtaga gattgcacat tttcgcagtt tcagcatgga aagatggaga aacaacgtaa
    12541 tttgctccag ctagaatggc atctctagca gttacggcat caagcacagt acctgcaccg
    12601 atacaaacac tctgatcgtc ctgatacaag tctacaagtt ccttgatgat ttgtcctgca
    12661 tactgattgg tataggcgat ttcaatagct ttgataccgc ccttgataca agcaatcgag
    12721 gcttgtagtc cttcttcctt tgtatttcct cgaatgacag cgacaatttt ctgttttttt
    12781 agttcaataa tcgtatctga tttggtcatg taattctcct aacgaatgat atcttgtgca
    12841 tttgccagta aattttcaat actagttgcg gaagtggaga gatggtctcc tggaagagtg
    12901 catttgagag ttgcgctcgc aactgcaaag tcaatggtag tttttaggga ggaatgatgg
    12961 agtagttggt aaagggcacc agatataaag gcatccccgc taccaattcg ttgatagact
    13021 gcagttttta gttggacaga ctcttcaaat cgttcttcca gagcataggc ttgatagaca
    13081 tttttgtctt gctcatcact agatcggagg gtatggaaaa tggccttgaa accataggct
    13141 tcttttaaaa gtcgcatgcg attttccacc tcttctaggc tagcactgtc tcttggaaac
    13201 atctctagat tttggtcatc aatcatgaga ggatcaatac cgaagcaata gtcagtaaaa
    13261 cgtgcaaact tagaaaattc atacttggct tctaggactg aaatcatctt tgttctcaga
    13321 ttgagatcca ttgaaacgac aattcctcgg cgcttggctt cttccaagag taggagaagg
    13381 atcgcacgga cctcttgacc gatagctacg gtgattccac taaaatgaaa atggctaatc
    13441 ccctgaaaga gagaatccat atctagcatg tttggccgaa tctggctgat actcgtatgc
    13501 ttacgatcgt agaaaacttc actttgacga caaccaaagc cgttctccaa atagtagagg
    13561 ccgattcgat cgccaagccg acagattgaa ctggtatcga tttggtgctg tttcaagaat
    13621 gtgagaaaac gatctccaat ctcgttggca gggagtgcgg taaagacttt cgttgagata
    13681 cccagggctt gcaaattaca agcgatgtta atttctgatc cgccaaaata agtcgaactg
    13741 gcaacatgat cgccgatact ggtggcatct aatggtgaga ttcgaattaa tggttctcca
    13801 aacagtaata ttttcgtcat ccattagttc ctttctggtt tagaatagct ttacttggct
    13861 aatacttctt tcaaataagc agcaacttca tcatcttggc agttttcaaa gaagtattct
    13921 tggaagtatt tgccagagat ggtgtctttg aggaagtctt ggtcgattat tttaaggatg
    13981 gtcatcaaat cattgtgggt gatttgtttc acctcgttta agatacgagc gttgcgttgt
    14041 tcagggatta cacgttctct tgggtagcca ccgcccattt cttcagcaaa taagcgttca
    14101 aacatcaatt tgagggtcag ttctgccccc cagccaaatc ctttggcata agggatagac
    14161 aaggcgttac caccattgat ttgagaataa aggtaagcgt cagttgggtc cactgctaga
    14221 ccacagacaa caccagggaa gctgtttaaa gcaagcatag cccctacacc cgtaccacag
    14281 ccggtaacaa caaagtcaac tgcctttgta tttaaaagga tggcagccat tagtccgttc
    14341 tgcacataag ttaattgact ttctccttct tctccacgca taccatagtt aaataattgg
    14401 tagccttttt tatctgtcgc ttcttttaga ctatcgtaaa taatgtgatt cttgctagct
    14461 tgactatttt cattgattaa tgcgattttc ataagatctt ctttctattt ttactcaggt
    14521 tgttttccga tgtaggctaa gataccgcca tctacatata ggatgtggcc attgacaaaa
    14581 ttgctggcat cactagcgag aaagacagca gggcccatca aatcttcagg atttccccaa
    14641 cgtgcagcag gtgtttttgc aatgatgaac tggtcaaatg ggtggcgaga accatcttct
    14701 tgcaactcac gaagaggtgc tgtttgagga gtggcaatat aacccggtcc aattccgtta
    14761 cattggatat tggctccacc gtattcagac gcaatgttgc gggtcaacat tttcaagccc
    14821 cctttagcag cagcataagc gctaactgtt tcacgtccca gttcgctcat catcgaacaa
    14881 atattgataa tctttccatg ccctttcttt atcatagaag gaataactgc ctttgaaacg
    14941 ataaatggtg cgtttaagtc aatatcgatg accttacgga aatcagcggc gctcatttcg
    15001 cacattggaa ctcggcggat aataccagcg ttattaacga ggatgtcaat gacaccaacc
    15061 tcttgttcga tttgcttgac catggcttgg ataccgtcct cgtctgtcac gtcacagaca
    15121 tatccatggg cttcgatgcc aacttcacga taagcttcaa tccctttatt gaccagatct
    15181 tgattgatat cgttaaagac aatagtagcg ccggcttcag cgtaggattt ggcaatagca
    15241 aatccaattc cataagaagc gccggtgatg agagcaattt ttccttttaa agaaaactgc
    15301 tcaattgaga atgatgtatt tgtcatttga tacttccttt caaatttgtt aagac
//
LOCUS       DAVGJJ010000045        15281 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000045 DAVGJJ010000000
VERSION     DAVGJJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.045"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..380
                     /locus_tag="VVE35_001276"
     CDS             <1..380
                     /locus_tag="VVE35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3061460.1"
                     /translation="TNLELSWCEGTSGILLYFELLYDKRYSNIISKFQQLAFKFNLIQ
                     SSCYCHGLSSLLQTLNYQDKLNLRENVIEILLSRSKRDKNGILVFECEESSDTLFDFG
                     IGNLGIYWSILGERFPFELKKEL"
     gene            380..1606
                     /locus_tag="VVE35_001277"
     CDS             380..1606
                     /locus_tag="VVE35_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3061461.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVTQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            2054..2710
                     /locus_tag="VVE35_001278"
     CDS             2054..2710
                     /locus_tag="VVE35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU3061462.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            2700..3023
                     /locus_tag="VVE35_001279"
     CDS             2700..3023
                     /locus_tag="VVE35_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU3061463.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            3127..3957
                     /locus_tag="VVE35_001280"
     CDS             3127..3957
                     /locus_tag="VVE35_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3061464.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            4111..4965
                     /locus_tag="VVE35_001281"
     CDS             4111..4965
                     /locus_tag="VVE35_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU3061465.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            5065..6438
                     /locus_tag="VVE35_001282"
     CDS             5065..6438
                     /locus_tag="VVE35_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU3061466.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAVDGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            6431..7492
                     /locus_tag="VVE35_001283"
     CDS             6431..7492
                     /locus_tag="VVE35_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU3061467.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            7494..8186
                     /locus_tag="VVE35_001284"
     CDS             7494..8186
                     /locus_tag="VVE35_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061468.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(8215..8784)
                     /locus_tag="VVE35_001285"
     CDS             complement(8215..8784)
                     /locus_tag="VVE35_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU3061469.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            8919..9533
                     /locus_tag="VVE35_001286"
     CDS             8919..9533
                     /locus_tag="VVE35_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU3061470.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            9535..11169
                     /locus_tag="VVE35_001287"
     CDS             9535..11169
                     /locus_tag="VVE35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3061471.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDRESEN
                     WLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANYQKQV
                     VDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLELSQKD
                     ANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERETLLK
                     QELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVDHRRT
                     DNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRNFEHQASYSDQRQSIGI
                     VNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            11181..12467
                     /locus_tag="VVE35_001288"
     CDS             11181..12467
                     /locus_tag="VVE35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061472.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATVSHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            12528..13061
                     /locus_tag="VVE35_001289"
     CDS             12528..13061
                     /locus_tag="VVE35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061473.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            13139..13609
                     /gene="nrdI"
                     /locus_tag="VVE35_001290"
     CDS             13139..13609
                     /gene="nrdI"
                     /locus_tag="VVE35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU3061474.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(13902..15164)
                     /locus_tag="VVE35_001291"
     CDS             complement(13902..15164)
                     /locus_tag="VVE35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU3061475.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFSAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         4434 a         2852 c         3405 g         4590 t
ORIGIN      
        1 ctacaaattt agaactatcg tggtgcgaag gaacttcagg aatcttactt tattttgagt
       61 tattatatga caaaaggtat tcaaacataa tttcaaagtt tcaacaactt gctttcaaat
      121 tcaatcttat ccagtcaagt tgttattgtc atggcttatc cagtttgttg caaactctaa
      181 actatcagga taaattaaat ttacgtgaga atgtgattga gattttatta agtagatcca
      241 aaagagataa aaatggtatt ttggtatttg aatgtgaaga aagttcagat acattatttg
      301 attttggtat cggaaatcta gggatttatt ggtctatcct tggggaaaga ttcccatttg
      361 aacttaagaa ggagttgtga tgaaagtatt tcttcaaaat agagatttta ggcaattaac
      421 tatcaaccag tggatttcaa cgcttgggga tacgattttt tatctggcct tcctgaatta
      481 tgtggcagat gcatcttttg cccctttggc gattttactc atcacgattt cagaaaccct
      541 gccccaagtt ctacaaatct ttctgggagt tttggcggat tttcaacatc atcgtgtctt
      601 gaaatacaca gtcattagtt ttgcaaaatt tttgctttac tctatagtgt ctttatcact
      661 ttcagggcag tccttttcct tgttattagt agcatttatt tgtctgatta acctcttgtc
      721 tgacacgttg agttattttt caggcgccat gctcactccg attttcatta gaattattgg
      781 gcaagaccat ctggcagaag ctattggatt taaacagtca actgttagtt tagtgaaaac
      841 aatcagtaat attctaggag gagtcttatt aggtattcta tccatccagt ttatttcctt
      901 gctgaacgct cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct
      961 cttgaaagta gaaaaaacga ttaactatca agaaggactc tctgtaaaat ccttttgcca
     1021 gcatttgctc caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt
     1081 tattatctct actagtcaag cagtgataaa tgttacagtt cctgtctcta ctctattttt
     1141 gaggaatcaa ccctttttga atttacaaac aggtcaatct cttgccttgc tatccacttt
     1201 tgaactgtca gccctgattg tcggaagcct tgtaagtggc tatctgcaac ataccatttc
     1261 cataaaaaca gctctctatg cctcacttgt cacccagttg cttcttctag taggatttgc
     1321 cacagttcgt tttgactgga ttctcatctt tagtaccttg gatgcctttt tcgcaggtgt
     1381 tctctctcct agattgcaag aactcgtttt taaacaaata cctgaggagt caatgggagc
     1441 agttcaatcc tctatcggtg ccattacggt tgttttacct agcttattta caatagcttt
     1501 ggtaaccatt gctactagct ttggaactct ggcagttagc tttgttttat tgctatttct
     1561 tctagttgcc tttgttatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat
     1621 aatatatgtt tgagactgga ttgccagcct ttttatgatg gtcaaaatca acagactttt
     1681 ttctcttttc tgaatgtgtt ataatagact gaatctaaaa tagtacgaaa caattgctaa
     1741 aacatttatt tatagaaatt aattttactt tcccaatcga tttgttctca tcttatttca
     1801 atctgctata taatagtcca tgctgtggct ggattctttt cagcctactt atactcttcg
     1861 aaactctctt caaaccacgt cagcttcacc ttgccgtagg tatggttact gacttcgtca
     1921 gttctatcca caacctcaaa acagtgtttt gagctgacta cgtcagttct atctgctttt
     1981 cattgagtac tattaagaca atatgaggag aaagatgaaa gaaaaaggat tttaggaggg
     2041 ggcgcaggca gccatgccaa cggctcttgg ctatgtcagt atcggcctgg cctgtgggac
     2101 tatcggtgcg ccctatgtga cacctgttga gatgggcttg atgagtctct ttgtttatgc
     2161 tgggagtgcc cagtttgcca tgttggcact gattgtggtt caagctcctg tggcagctat
     2221 tgctatgacg gtttttctaa tcaacttgcg tctctttttg ttgagtttac acgcatcgac
     2281 ttatttccgt cataccagtc tttggtacaa tatcggtatg tctagtatct tgacagatga
     2341 gacctatggc gttttgatgg gtgaattggc ccatacagac aaggtaaatc ctatgtggat
     2401 gcacggaaac aatcttaaca gctatgtggc ttggtttgtg gggacagtag tcggaacggc
     2461 tctgggtggc ctgctaccaa atccagaaat ctttggcttg gattttgccc tggttgggat
     2521 gtttattggt atttttgctt cgcaatttca gattatgcaa agacggattc ctgtccgcaa
     2581 tctgctcatt atcctagcag ttgttgcggt gtccttcttt ttgctcttga cagtggtgtc
     2641 tcagtcacta gctgttctgt ttgcgacgct acttggttgt agcatggggg tggttttaga
     2701 tggtcagtaa gtatctttta ttagcagtta ttttctctgg cttggtgact tggattctcc
     2761 gtatgattcc cttcatctta gtcaagtata agggcttgcc tgcaatcgtt gagcgttttt
     2821 tgaagttctt gcccgtttcc attatctttg ccttgattct ttcaagcgta gtgacaggta
     2881 aggttgggag ccttcctcaa attaaatggc tagacttctt agccgtcttt ccaacagctt
     2941 gggtagcctt tcgctaccgc aatctagtcg gaacagttct ctttggagtg gtcttgattg
     3001 ccatcttgcg tttggtcttt taaattaccc ataaaaaaaa cttatcacag agatagatat
     3061 catataatgg cgtaaatgct ctttttctgt taagattata aggtattcta ttttggagga
     3121 aatgacatga aaaaaatcgt taaatactca tctcttgctg ccctaggact tgttgctgca
     3181 ggtgtgcttg cggcttgctc agggggtgct aagaaagaag gagaagcagc tagcaagaaa
     3241 gaaatcatcg ttgcaaccaa tggatcacca aggccattta tctatgaaga aaatggcgaa
     3301 ttgactggtt acgagattga agtcgttcgc gctatcttta aagattctga caaatatgat
     3361 gtcaagtttg aaaagacaga atggtcaggt gtctttgctg gtcttgacgc tgaccgttac
     3421 aatatggctg tcaacaatct tagctacact aaagaacgtg cggagaaata cctctatgcc
     3481 gcaccaattg cccaaaatcc taatgtcctt gtcgtgaaga aagatgactc tagtatcaag
     3541 tctctcgatg atatcggtgg aaaatcgacg gaagtcgttc aagccactac atcagctaag
     3601 cagttagaag catacaatgc tgaacacacg gacaacccaa ctatccttaa ctatactaag
     3661 gcagacttcc aacaaatcat ggtacgtttg agcgatggac aatttgacta caagattttt
     3721 gataaaatcg gtgttgaaac agtgatcaag aaccaaggtt tggacaactt gaaagttatc
     3781 gaacttccaa gcgaccaaca accgtacgtt tacccacttc ttgctcaagg tcaagatgag
     3841 ttgaaatcat ttgtagacaa acgcatcaaa gaactttata aagatggaac tcttgaaaaa
     3901 ttgtctaaac aattcttcgg agacacttat ctaccggcag aagctgatat taaataattt
     3961 cttgaaatca tccattctga gtaaggtggg tggtttctca gtttttaggg atatagtttt
     4021 aaactatata tctgactatc taataacaat ttgtgaaatc aaacaaattg tgagatacta
     4081 gtacggtatt atttttaagg agaaagaatc atgaaaatca aaaaatggct tggcctagca
     4141 gcccttgcta cagtcgcagg tttggctctt gcagcttgcg gaaactcaga aaagaaagca
     4201 gacaatgcaa caactatcaa aatcgcaact gttaaccgta gcggttctga agaaaaacgt
     4261 tgggacaaaa tccaagaatt ggttaaaaaa gacggaatta ccttggaatt tacagagttc
     4321 acagactatt cacaaccaaa caaagcaact gctgatggcg aagtagattt gaacgctttc
     4381 caacactata acttcttgaa caactggaac aaagaaaacg gaaaagacct tgtagcgatt
     4441 ggagatactt acatctctcc aatccgcctt tactcaggtt tgaatggaag tgccaacaag
     4501 tacactaaag tagaagacat cccagcaaac ggagaaatcg ctgtaccgaa tgacgctaca
     4561 aacgaaagcc gtgcgcttta cttgcttcaa tcagctggct tgattaaatt ggatgtttct
     4621 ggaactgctc ttgcaacagt tgccaacatc aaagaaaatc caaagaactt gaaaatcact
     4681 gaattggacg ctagccaaac agctcgttca ttgtcatcag ttgacgctgc cgttgtaaac
     4741 aataccttcg ttacagaagc aaaattggac tacaagaaag cacttttcaa agaacaagct
     4801 gatgaaaact caaaacaatg gtacaacatc attgttgcga aaaaagattg ggaaacatca
     4861 cctaaggctg atgctatcaa gaaagtaatc gcagcttacc acacagatga cgtgaaaaaa
     4921 gttatcgaag aatcatcaga cggtttggat caaccagttt ggtaataaga aacagggagg
     4981 tgggagagag aattccacct cttgcttttg tatagagcac ggagtgtaaa gaagactatt
     5041 cgttcataga aaggtagaga gaatatggtt tttcctagcg aacaagaaca gattgaaaaa
     5101 tttgaaaagg atcatgtagc ccaacattat tttgaggttt tgcgtacctt gatttctaag
     5161 aaatcagtct ttgcccagca ggttggactc aaggaagtcg caaattatct gggtgagatt
     5221 ttcaagcgtg ttggagctga agtggagatt gatgagagct atacagcgcc ctttgtcatg
     5281 gcacatttca agagttcgcg tccagatgcc aagaccttga ttttctataa ccactatgac
     5341 actgtgccag cggatgggga tcaggtctgg acagaggatc cttttacgct ttcggtccgc
     5401 aatggcttca tgtatgggcg tggggttgat gacgacaagg gtcatatcac agctcgcttg
     5461 agtgctttga gaaaatatat gcagcaccat gatgatttac ctgtcaatat cagctttatc
     5521 atggagggag cggaggaatc ggcttcaaca gacctagata agtatttgga aaagcatgca
     5581 gacaaactcc gtggggcgga tttgttggtc tgggaacaag ggaccaaaaa tgccttggaa
     5641 cagctggaaa tttctggtgg caataagggg attgtgacct ttgatgccaa ggtaaaaagc
     5701 gctgatgtgg atatccactc gagttatggt ggtgttgtgg aatcagctcc ttggtatctc
     5761 ctccaagcct tacagtctct tcgtgctgtg gatggccgta tcttggttga aggcttgtac
     5821 gaagaagtac aagagcccaa tgaacgagaa atggccttgc tagaaactta tggtcaacga
     5881 aacccagagg aagttagtcg gatttatgga ttggagttgc ctctcttaca ggaggagcgg
     5941 atggcctttc taaaacgttt ctttttcgag ccagcgctta atatcgaagg aatccagtct
     6001 ggttatcaag gtcagggtgt taagactatt ttgcctgcag aagccagtgc caagctagag
     6061 gttcgtctgg ttccgggctt agaaccgcat gatgttctgg aaaaaattcg gaaacagcta
     6121 gacaaaaatg gctttgataa ggtagaatta tactatacct tgggagagat gagctatcga
     6181 agcgatatga gcgcaccagc cattctcaat gtgatcgagt tggccaagaa attctatcca
     6241 cagggcgttt cagtcttgcc gacgacagcg gggacaggac ctatgcatac ggtctttgat
     6301 gccctagagg taccaatggt tgcattcggt ctaggaaatg ccaatagccg agaccacggt
     6361 ggagatgaaa atgtgcgaat cgctgattat tacacccata tcgaattagt agaggagctg
     6421 attagaagct atgagtagag atattatcaa gttagatcag atcgatgtga cttttcacca
     6481 aaagaagaga accattacag cggttaagga tgtgaccatt cacatccaag aaggggatat
     6541 ctacggaatc gttggatatt ctggagcagg gaaatcaacc cttgtacggg tgattaacct
     6601 cttgcaaaaa ccatctgcag ggaaaattac cattgacgac gatgtgattt ttgacggcaa
     6661 ggtgaccttg acggcagagc agttgcgtcg taaacgtcaa gatatcggaa tgattttcca
     6721 gcattttaac ctgatgagcc aaaagacagc agaggagaat gtagcctttg cccttaaaca
     6781 ctctgaactc agcaaggaag aaaagaaggc taaagtagct aagttgttgg acttagttgg
     6841 tttggcagat cgtgctgaaa actacccttc acaactatct ggagggcaaa aacagcgtgt
     6901 ggcaattgcg cgtgccttgg ccaatgatcc aaaaatcttg atttcagacg agtcaacttc
     6961 tgcccttgac cctaagacaa ccaagcagat tttggccttg ttgcaagatt tgaaccaaaa
     7021 attaggattg acagttgtct tgattacgca tgaaatgcag attgtcaaag acattgccaa
     7081 ccgtgtggcg gttatgcagg atgggcattt gattgaagag gggagtgtcc ttgaaatctt
     7141 ctcaaaccct aaacaacctt tgactcaaga ctttatctca acagccacag gtattgacga
     7201 agccatggtc aaaatcgaga agcaagaaat cgtggaacac ttgtctgaaa acagtctctt
     7261 ggtgcaactc aagtacgctg gagcttcaac agacgagcca cttttgaatg aattgtacaa
     7321 gcattaccaa gtaatggcta atattctcta tgggaatatc gaaattctcg atggtactcc
     7381 tgttggagaa ttggtggtgg tcttgtcagg tgaaaaagca gccttggcag gtgcccaaga
     7441 agccattcgt caagcaggtg tacaactaaa agtattgaag ggagtacagt aagatggaat
     7501 cattgattca aacctattta ccaaatgtct ataaaatggg ttgggctggt caggcaggct
     7561 ggggaacggc tatctactta actctttata tgacagttct ttccttcatt atcggaggct
     7621 tcttggggct agtggcaggt ctctttctcg tcttgacagc gccaggtggt gtcttggaga
     7681 ataaagtcgt attctggatt ttagacaaaa ttacctcaat ttttcgtgcg gttcccttta
     7741 tcatcctctt ggcaatcttg tcaccacttt ctcacttgat tgttaaaaca agtatcgggc
     7801 caaatgcagc ccttgtccca ctttcttttg cagtctttgc cttctttgcc cgtcaggtgc
     7861 aggttgtctt ggctgaattg gatggcggtg tcattgaggc ggctcaagcg agcggagcga
     7921 ctttctggga catcgtgggt gtttacctat cagaaggtct tccagatttg atccgtgtga
     7981 cgactgtgac cttgatttcc cttgttgggg aaacagctat ggccggtgcg gttggagctg
     8041 gtggtatcgg taacgtagcc atcgcttatg gatttaaccg ctacaatcac gatgtgacca
     8101 tcttggcaac cattattatc attttgatta tctttgcaat ccaattcttg ggagatttct
     8161 tgactaagaa attgagccat aaataaaaag agccgcgtgg ctctttttaa ctgatcagat
     8221 tttctgtgca aattttttac tcaaggcttg tccaatcaag gcacccacta gggctccgat
     8281 gacaatactt gcgataaata gaaggacagt tccagggttt ggagcgacca tgatacggtc
     8341 gatatattct tgggattttc ctcttgccag aagagtagcc atataggctt tgggcgcaat
     8401 ccacataagc aagattggtc ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt
     8461 ctttgttttg tccttgtatt ttcctaaatg agctactcca tctgctagga ggccacagat
     8521 aattccagga aggaaggaac cagcaccgtg tttagttccc aagaaaaaga gggcaatgac
     8581 aaggccgata gtggtaatgg ctccaaagcg cggaactttt gcgactagga tcatatagac
     8641 gctaccgccg acaagggcag taaaggcagg cgcataaaac atgtttccag tttggtcaaa
     8701 gagattgccc aaaaggacac caatccccat gcagaggaag taaagaactg caaaaagcag
     8761 tgtagttaag atagatttct tcatgaattg tctcctgata attttttcac aattctcatt
     8821 gtaccacggt ttggtgggat tgtaaatgga tactagaagt ttttgaaaac gcttgaaata
     8881 tgatataatg gtttcatagt tatttttagg ggaatatcat gggtagattt ttagactttg
     8941 tctttaatcg tttcttttta gggatgattg cgacagcctt cttttggcta ttaactttag
     9001 taggagggat tatccttggt ctagcgccag ctagtgccac cttgatgagc ttatatgcag
     9061 aacatggtta tagctttcgg gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa
     9121 attttgtctc aagcaacctg attttctata gctttttagg tgtgggtcta gttttgacct
     9181 atggtttgta tctattggtg caattgcctc atcagaccat tgttcatttg attgcgaccc
     9241 ttttgaatgt cctagtagtt gccctgatct ttttggctta tacagtatct ttaaaattac
     9301 aagtttattt tgccttgtcc tatcgaaata gtctcaaatt atccttgatt ggcatcttta
     9361 tgagtctagc agctgtggcc aaggttctcc ttgggactgt gctacttgta gcaattggtt
     9421 actatatgcc tgccctgcta ttttttgtag gaattgggat gtggcatttc tttatcagtg
     9481 atatgttgga acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag
     9541 cagttttggc tacatacgct tctaagaacc tatagttcag tgatgatcat tatcattgcg
     9601 agttttgcaa tcttactctc ttacgctgac tgggattcac gtgaaaagga agcccagaga
     9661 gtagcccagc gtgtaactgc tagaacagtg agtgaaattg aatattacca tagagagtca
     9721 acccagatag ctcaggcttt agttgaaaat caagctcgta ttgagggaat ctataaatac
     9781 tttagcctta gcatgccaga ctatttttac tggcaattag agcggaaagc ttcgccttat
     9841 atatcagtct ctctgtatga aaatgttgat gacctctatg ttcgaaatga ttttgtaact
     9901 ggggtggcca ttgcttttca agattacaag gaagtctatg tttctactaa agacaaacgt
     9961 agtggagaaa aaatcagggc tgaggatttc aaaccagcag gaaatagttt tgccattccg
    10021 gtgtcagatc aagatttagg agtgatttac atctccttgg atcctgctgt tttataccat
    10081 gccattgata atactagagg tcatactccg atggcagtaa cagtgacctc accttttgat
    10141 acggagattt ttcatattgg tgagacagtt gatagggaga gtgaaaattg gctagttggc
    10201 ttaacttctc atggatatca ggttcaggtg gcagttccta aaaactttgt tttacaagga
    10261 acagtgacta gctctgcttt gattgtgggt ttgagccttc tctttattgt cattctttat
    10321 ctgactttga ggcagacttt tgctaattac caaaagcagg tagtggattt agtggaatcc
    10381 attcaagtca ttgctcaagg cgaagagggg cgtcggattg acatttccga aaaagatcag
    10441 gaattactcc taatcgcgga gacgaccaat gatatgttgg atcgattgga aaagaatatc
    10501 catgatattt accagttaga gcttagtcaa aaagatgcca atatgcgagc cttgcaggcg
    10561 caaatcaatc ctcattttat gtataatacg ctggagttct tgcgcatgta tgcagttatg
    10621 cagagtcaag atgagttggc agatatcatt tatgaattca gtagtctctt gcgtaacaat
    10681 atttccgacg aaagagagac cctcctcaaa caggaattag aattttgccg taaatacagc
    10741 tatctctgca tggttcgcta tcccaagtcc attgcctatg gtttcaagat agatccagag
    10801 ttagagaata tgaagattcc caagtttacc ttgcaaccgc tggtagaaaa ctatttcgcg
    10861 catggtgttg accacaggcg gacagataat gtgattagca tcaaggctct taaacaggat
    10921 ggttttgtgg aaattttggt ggtcgataat ggtagaggaa tgtcggctga aaagctggca
    10981 aatatccgag aaaaattaag tcagagaaac tttgaacacc aagccagcta cagtgatcaa
    11041 aggcagtcta tcggaattgt caatgtacac gagcgttttg tgctctattt tggagaccgc
    11101 tatgccatta ctatagagtc tgcagagcaa gccggtgttc agtatcgtat tacaattcaa
    11161 gatgagtaga aagggagaaa atgtataaag tattattagt agatgatgag tacatggtga
    11221 cagaaggtct gaagcgtttg attccctttg ataagtggga tatggaggtc gtcgcaacag
    11281 tcagtcatgc cgatgaagct ctagaatatg ttcaggaaaa tcctgtcgat gtcatcattt
    11341 ccgatgtcaa tatgccagac aaaacagggc ttgatatgat tcgggagatg aaagagatct
    11401 taccagatgc tgcctatatc ctgctctcag gttatcagga gtttgattat gtaaaaagag
    11461 caatgaacct tagtgtggtg gactatttgg tcaagcctgt tgataaggta gagctgggaa
    11521 atctgctgga gaagattgca ggtcagctcg gcgagagagg gaagaaaagt cagactctta
    11581 gtcaagaatt agacgaggct ggatttgtta gttatttagg ggataaggag aattggtgga
    11641 taggtctatc caaggaaaaa caaggttcct tcgccattcc ctactatgtc ttgggccaag
    11701 actggcagat tttcatttct gaccaacccc tagatggttt agtcgttaca ccttttgaag
    11761 ctccttatca agaacacttt gaacgctgga agctgaatgc tgagaaaacc ctcttttacg
    11821 gttctgtaaa tctgcagcag tctgagagtc tctttgccta ttacgaaccg atttataggg
    11881 ttatcattca gggaaatctc aatcaaatcg tagaagagtt aaatctcttg gagaaggtag
    11941 ttcttgaaaa tacgccgcgt gttccgatta ctaaacagct ttttattcag tttgtcatgg
    12001 atgttttcca tttatttgaa catctcaaag ctgatgacct gacagacatc gtcaaaacca
    12061 ttcatgctat tcaatccttc gatgaattgg ttccttatat caaggaaact ctgagcagct
    12121 ttttcggtca ataccgtatg aatgaaaatg tggtcagtgt gctggaagtc attggtcgtg
    12181 attaccaaaa agagctttcc ctcaaggata tcagtaaggc tctctttatc aatcctgtct
    12241 atctagggca gttgattaag cgtgaaaccg attcgacttt tgcagagtta ctaaacaaac
    12301 aacgtattaa ggctgcccaa caacttttgc tttcaactag tgacagcatc gaagatattt
    12361 gttatgctgt tggttacagt aaccttggat atttctataa agttttccga aaattgtgcg
    12421 gaaaatcgcc aaaagcctac cgaaaacagg tagaaactat actataagat ttgtattcct
    12481 ttacaaaatg tgctataata taaatattat tattaggagg ttctatcatg aaaaagaaac
    12541 caatttatct atgggtcttg ctaatcttgt ctgcccttat ttcagttccg tctctgtttg
    12601 gaattgtgag tcccttgcct agtaaagaag cccttcgtac tgctcagaaa caagttgcag
    12661 gggtcaatgc tcagcaatta gaagaccagc ttaattatac ctatagagta gcagaagcat
    12721 ctcattctat ttttaatgtt gctttgattg tgctatctac tattttagtg gtcgtagcga
    12781 ttgtcttcct tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt ggctatgttt
    12841 tgttagctat tattggttcg atttatggct atgttggttt acaagacgct gtgcagttgg
    12901 ttcaagatga gagcatgcgt ttgacagtga gtattggttc aaaagctgtt agcattttct
    12961 atatcgtcat caatgttctc ttcctagccc ttgtcttcta taagatgtgg cgacaacaga
    13021 aagccttggc agaagaagag gaaacagaag agcttgccta agtattgaca aaaaagaact
    13081 atcaagatac aatcttggta gttgtttttt gattatagat aaaatcatgg aggtacatat
    13141 gaagacaatt tctttagttt atattagttt gagtggcaac actgaaagtt ttgtgacgcg
    13201 cttgaaagac tatctcttgt cccagtacaa agggattgag gttcaaaaga ttcatatcaa
    13261 ggatttggtc aaggaaggca aaaatttcta tgaaatggac catccctatg tcgctttttt
    13321 gccgacctat ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga ttttgacgac
    13381 accggtggga gattttattg cctatggtaa caatgctagt aagtgttttg gtgtggttgg
    13441 gtcaggaaat cgtaacttta ataaccaata ctgcctaaca gccaagcaat acagtcaacg
    13501 ctttggcttc cctgtattgg ctgactttga aatgcgaggc atgctggaag atatcaaaca
    13561 tgtcgcagct attatcgcag atttgtatga gttggaaaag gagaattaat actcaatgaa
    13621 aatcaaagag caaactagga agctagtcgc aggttgctca aagcacagct ttgaggttgc
    13681 agatagagct gacgtggttt gaagagattc tcgaatagta taaagttctt ataagaggtg
    13741 gttgaagttg ttcaactgtt ttttgagtat aaacagtctt taagaacgta aaaaagcata
    13801 agttcaggtc ttcaaaacct tgttcttatg ctttttatca tagaaatatt tgctgaattt
    13861 tctcttgaaa tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc ttttccgatt
    13921 gcgagtgcgt agataaagaa gatggcgaag ccaaagagga aaatcaatcc cgcaatggca
    13981 aggtgtttga gccaagaagg aagatttttg ttttcacgcg ttaccaaggc gtaattcaaa
    14041 agagcaaaga aaggtgttgt caggaaagat ccaatcatgg caaagcggag catggttgaa
    14101 acctgaccag cgaagaactt gataatgacg ataccgatga tagcagtgat ggtcatccag
    14161 atgttcaaag atttacgatt gtcctctttt tgactgatta gcagtcggag agattcctga
    14221 ttaacgcgag aatagccatc gataacagtt ataactgttc caaagataca gaggaaggca
    14281 ataaaggtaa tcaagtaacg ggaccattcg ccaagaacag aggcatacat gcccacgaat
    14341 tgagagatgt atttggctga agcagcttca accgcctgcc ctgtaggata ctgaatcagt
    14401 gctcccagtg ccacaaagaa gacggctagg atagctgttc caatataacc agtgttaaag
    14461 tcaaacagag cgtcttctgt gttaaagttg acggtctttc tcttttcagc tgaccaaagt
    14521 gaattgatgg ctgaaatttc aataggagcc ggcatccatc ctaggaggga gacgatgaag
    14581 ggcagagctg ccatttgcca aggtgtcttc tcgacaaaat cagaactgta ttctggatgc
    14641 ttgaccgctg ctatgataac tgccagaaca gtcgcaatgg ttaaggcaga cataatccat
    14701 ttgaccatgc cgtctaaaag tttgtagcct ccaaagagta gcatagccca aatgattgca
    14761 acgagaatga gggaccactg agtaatgcta agtccaatca ttgggaaggc actggcgatg
    14821 atagctgagc acagaatggc aacaccagcc gtgttgacca tagccgaaaa gacattgagg
    14881 ataaagaaaa tccagagata gagttttcct ttttcggcat aaccttcaac caaagtcttt
    14941 ccagtatcag ctgtgtattc agcactaaaa cggaaaaatg gatatttaaa gacattggct
    15001 aagatgacca agagaagtag agaccaaccg taagaaccgc cagcttgagt tgaggataca
    15061 atgtgggaac ctccaacagc ggcagttgcc attaggattc caggtcccat tgcttttagc
    15121 ttgcttgccc aggttgattg atttaaggaa atagcttgcg acatgataaa tactcctttt
    15181 tgaattttca gaataatctg tatatgtaat ctatattcta caaaaaattt tggaaaaatg
    15241 caagaatatt ttgaaaaaag atagaaaatt tacaaagaag a
//
LOCUS       DAVGJJ010000046        14825 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000046 DAVGJJ010000000
VERSION     DAVGJJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.046"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..379
                     /locus_tag="VVE35_001292"
     CDS             <1..379
                     /locus_tag="VVE35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU3061476.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
     gene            801..1175
                     /locus_tag="VVE35_001293"
     CDS             801..1175
                     /locus_tag="VVE35_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU3061477.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            1633..2346
                     /locus_tag="VVE35_001294"
     CDS             1633..2346
                     /locus_tag="VVE35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU3061478.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2404..3006
                     /locus_tag="VVE35_001295"
     CDS             2404..3006
                     /locus_tag="VVE35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061479.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3144..3797
                     /locus_tag="VVE35_001296"
     CDS             3144..3797
                     /locus_tag="VVE35_001296"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU3061480.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            3807..4271
                     /locus_tag="VVE35_001297"
     CDS             3807..4271
                     /locus_tag="VVE35_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3061481.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4284..5552
                     /locus_tag="VVE35_001298"
     CDS             4284..5552
                     /locus_tag="VVE35_001298"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU3061482.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5566..5826
                     /locus_tag="VVE35_001299"
     CDS             5566..5826
                     /locus_tag="VVE35_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061483.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5837..7198
                     /gene="pdrM"
                     /locus_tag="VVE35_001300"
     CDS             5837..7198
                     /gene="pdrM"
                     /locus_tag="VVE35_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU3061484.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            7352..8320
                     /locus_tag="VVE35_001301"
     CDS             7352..8320
                     /locus_tag="VVE35_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU3061485.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8336..9328
                     /locus_tag="VVE35_001302"
     CDS             8336..9328
                     /locus_tag="VVE35_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU3061486.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEVFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9546..10589
                     /locus_tag="VVE35_001303"
     CDS             9546..10589
                     /locus_tag="VVE35_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU3061487.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSLAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMNLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10653..12338
                     /gene="lpdA"
                     /locus_tag="VVE35_001304"
     CDS             10653..12338
                     /gene="lpdA"
                     /locus_tag="VVE35_001304"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU3061488.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESSLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12402..13391
                     /locus_tag="VVE35_001305"
     CDS             12402..13391
                     /locus_tag="VVE35_001305"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU3061489.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13466..14536)
                     /gene="xerS"
                     /locus_tag="VVE35_001306"
     CDS             complement(13466..14536)
                     /gene="xerS"
                     /locus_tag="VVE35_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU3061490.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         4562 a         2667 c         3225 g         4371 t
ORIGIN      
        1 aagaaacaaa aatggtcaag ctgataccaa tcaaacggaa aaaccaagcg aggagaaacc
       61 tcagacagaa aaacctgagg aagaaacccc tcgagaagag aaaccgcaaa gcgagaaacc
      121 agagtctcca aaaccaacag aggaaccaga agaatcacca gaggaatcag aagaacctca
      181 ggtcgagact gaaaaggttg aagaaaaact gagagaggct gaagatttac ttggaaaaat
      241 ccaggatcca attatcaagt ccaatgccaa agagactctc acaggattaa aaaataattt
      301 actatttggc acccaggaca acaatactat tatggcagaa gctgaaaaac tattggcttt
      361 attaaaggag agtaagtaaa ggtagcagca ttttctaact cctaaaaaca ggataggaga
      421 acgggaaaac gaaaaatgag agcagaatgt gagttctagt tctcattttt ttcatgaaaa
      481 tgtgcaaaat atagtagatt gaaactagaa tagtatacct ctacttctaa aacattgtta
      541 gaaatcgatt tgactgtcct gttcttattt cattttacta tatcttaaca gatagtgtaa
      601 ataaagataa actatttact ggctaattaa tcagttaaac actagttaag gagtaatgat
      661 gaaaaaaaga acaatactat tattgatggc cagtctgtta gctcttgtct taggagcatg
      721 tggtttcttg gacatattga tcctggatca ttctcatcag gattactctt tactgctatt
      781 ttagaaactg gggtggtttg atggaaagta ttggtcttgt tatcgtttca cattccaaac
      841 acattgcaga aggtgttgtt gaactgatta gtaaagtagc taaagatgtt ccgattactt
      901 atgtaagagg aaccgagggc ggaggaattg gaacgagttt tgaacaagta gatagggttg
      961 tttccgaaaa tccagcagat actttacttg ccttttttga cctaggttct gctaaaatga
     1021 acttaaaaat ggtgactgat ttcagtgata aaagtatcat catcaacagg gttccaattg
     1081 tagaaggtgc ctataatgca gctgctcttc ttcaggctgg tgcagaactg tcagttattc
     1141 aaacacagtt agcggagctt gaaatcaata aataaggaat tttactataa ctctttttat
     1201 aggtaagcta ttgattatct caactataaa atgttaagta aatgatttca aaatcagaaa
     1261 gggattgctt tatgcagttc ctttttattt taacaggagt gaaactatag tgtttctaaa
     1321 ttgtgaatca atcaaaactg attgtgatgg ggctattcta gctttagaaa ccttcaaaaa
     1381 ttaaaattta aggcaatcaa ttacttggaa gagtatgaaa gcatttagtt tataggaatt
     1441 ctaggtctag aattacatat atatttatga agacggggtg ttcgatagtt agtattgttc
     1501 tattctgaaa gatttgagct gtcagttgta tagaaagtgt tcgaattttt ttaagtgatt
     1561 aaattagtta attgtatgag gtgctttatg atataatgtt cttaatgaat tttcagaaag
     1621 gaaaacctca aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat
     1681 tttgatgctg ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat
     1741 attcaggctt ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag
     1801 ctgaagaaaa tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat
     1861 tttggacagg aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag
     1921 cagggacaaa cactatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat
     1981 aacttgtatg ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct
     2041 ggtctagcac cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg
     2101 gatgctcttt tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg
     2161 ctgatgattg gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgac
     2221 actatctggt ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa
     2281 gtctattctt accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca
     2341 ttttaaagga gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt
     2401 gaaatgtctg aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt
     2461 caattagcaa atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct
     2521 ggcgatttaa tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg
     2581 agaagggatt cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt
     2641 actaatcgaa aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat
     2701 gacttttttg atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat
     2761 aatattaaag agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat
     2821 tttcgatttt tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg
     2881 caagtttcta aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca
     2941 gaaaagataa tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa
     3001 aaatagccag tctttataaa ctgtattcac gtattcaaat cgtatatata gtcgatttat
     3061 taaatcttta gataaactgt aaaaagtggt ttccatagcc actttttgct ataatagagg
     3121 cagtaaaaat gaaggagtcg gctatggaac actcgtcttg gcatgctttg attaaggcgc
     3181 aattacctga gggttatttc gggaaaatca atcagtttat ggagcaggtc tattctcagg
     3241 ggattattta tccacccaag gaaaaggttt ttcaggctct cttgacaaca ctgcttgaag
     3301 aagttaaggt ggtaattcta gggcaagacc cctatcacgg accaggtcaa gcgcagggct
     3361 tgagtttttc tgtacctgac tctatcccag ctccaccatc cttgcaaaat atcttgaaag
     3421 aattgtcaga tgatatcgga gttaagaaat ctcatgattt gacagcttgg gctgagcaag
     3481 gagtcttgct tcttaatgct tgtttgactg ttcctgctgg acaggccaat ggtcatgctg
     3541 gtcaaatctg ggagcctttt actgatgctg tgattcaggt ggtcaatcat ctagatagac
     3601 cagtcgtttt tgtactctgg ggagcttatg cacgtaagaa gaaggcatta gttaccaatc
     3661 ctcatcactt gattatcgaa tcagcccatc caagtccttt gtctgtttat agaggatttt
     3721 ggggttccaa gcctttttcc aaggccaata cattcttaaa agagacagga caagagccaa
     3781 tcgattggct tagataagga gaaaatatgc ctcagttagc gacgatttgt tacattgata
     3841 atgggaaaga actgctcatg ctccatcgta ataagaaacc caatgatgtc catgaaggga
     3901 aatggattgg tgtgggtggt aagctagaga gaggagagac gccccaggaa tgcgcggtac
     3961 gtgaaatcct tgaagaaaca gggctcaaag ccaagccagt tctaaaaggt gtcatcactt
     4021 ttcctgaatt tacaccagat ttagactggt acacctatgt ttttaaggtg acggagtttg
     4081 agggcgactt gattgactgc aatgagggga tgctagaatg ggttccctat gatgaggttt
     4141 tgagcaagcc gacttgggaa ggtgaccaca cctttgttga gtggctttta gaggataaac
     4201 ccttcttttc agccaagttt gtttatgatg gggataaatt gttggatacc caagttgatt
     4261 tctatgaata aaggagaaag cagatgctac taatcaaaaa tggtcgtgta atggatccca
     4321 agtctggttt ggatcaagtt tgtgatgtct tagttcaaga tgggaaaatt atcaaaattg
     4381 cgcctgagat cacggaagaa ggagcagaaa cgattgatgc tactggtcat gtggttgctc
     4441 ctggtttggt cgatattcat gtgcatttcc gtgaacctgg tcaaacacat aaagaagaca
     4501 ttcatactgg tgccctagca gccgctgcag gtggttttac tactgtcgtc atgatggcta
     4561 ataccagtcc aaccatttca gacgtggaga ctttgcaagc agttctccag tcagctgcca
     4621 aagagaagat taatgtcaag acagttgcga ccattactaa aaactttaat ggtaaaaact
     4681 tgactgactt taaggcactc ttagaagctg gtgcggttgg tttctctgat gacggtattc
     4741 cgcttgagag cagtaagatt gtcaaggaag ccatggagga agccaaaaaa ctcaatacct
     4801 ttatcagcct tcatgaggaa gatccaggtt tgaacggtgt tcttggcttt aatgaaaata
     4861 ttgctagaga acatttccat atctgcggtg ctactggggt ggctgagtac gctatgatgg
     4921 cgcgtgatgt catgattgcc tatgcaacta aagcccatgt tcacatccag catttgtcta
     4981 aggaagaaag tgttaaagta gtggagtttg ctcaggggtt aggtgcagaa gtcacagcag
     5041 aagtagcgcc acagcatttc tctaagacag aagcactttt tttaacacaa ggtagcaatg
     5101 ctaagatgaa tccaccgctt cgtttggaat cagaccgtcg tgccgttatc gaaggtctca
     5161 aatcaggtgt catcacagtt attgcgactg accacgcgcc tcatcatgta gatgaaaaaa
     5221 atgttgagga tattaccaaa gcgccatctg gtatgactgg cttagaaaca tccctgtctc
     5281 tcagcttgac ctatttagta gaagctggtg agttgagctt gatggaatta cttgaaaaaa
     5341 tgacatacaa cccagccaag ctttacaact ttgaagcagg ttacttggct gagaatggtc
     5401 cagcagatat cactattttt gatgccaagg ctgaccgcct tgtggactcc cattttgctt
     5461 ccaaagcagc taattcacca ttcatcggtg aaaccttaaa agggcaggtt aaatatacca
     5521 tctgtaaggg acaaatcgtc tatcaagctt gataggagtt agaatatgaa ttattttcga
     5581 aaacgtaggg agagacaagc gaaatctaat agtggaattt actgtcccgc agctaatcgt
     5641 tccaaaattc aatatgaaac taaagaaaaa gctgaccatg cggttcaata tgataacgga
     5701 ggtttagtta gaagctatta ttgtcgtaca tgtgcgtgtt ggcatactac ttcaaaatct
     5761 aataaacctc cgctaagttt gaaaaactat ggtctgaaac tctttggttt agataaatca
     5821 gaatagaaag agaagtatgt ataagacaaa gtgtttacga gagaagttag tattattttt
     5881 aaaaattttc ttcccaatcc tgatttacca atttgccaat tattctgcct cttttgttga
     5941 tactgcaatg acaggtcaat acaacactat ggacttggct ggtgtatcta tggcaaccag
     6001 tatctggaat cctttcttta catttctaac agggattgtg tcagccttgg tgcctatcat
     6061 tggtcaccat cttggtcgag gaaaaaagga agaagttgcg tctgattttt accaatttat
     6121 ttatttggcc ttgggcctat ctgtggtatt gctggggatg gtacttttct tggcaccaac
     6181 aatcttgaat catattgggt tagaagcagc agtagcggca gtagcggttc gctatctttg
     6241 gtttttatct atcgggatta tccccttgtt gctctttagc gtcattcgtt ccttgctgga
     6301 ttcgctgggc ttgaccaaac tgtccatgta cctcatgctt ttgttactcc ctctcaatag
     6361 cggatttaac tatctcttga tttacggtgc ctttggtgtt ccagaactgg gaggggctgg
     6421 tgctggttta ggaacatcct tggcctactg ggtcttgctt gggatttctg ttctggtttt
     6481 atttaaacag gagaagctca aagccttaca ccttgagaaa cgaattccac ttaatatgga
     6541 taaaattaag gaaggagttc gtttaggtct gcctattggg ggaactgtct tcgcggaagt
     6601 ggctatcttt tcagtggttg gcttgattat ggctaagttt tcgtccttga ttatagctag
     6661 tcaccagtca gctatgaact tttcaagtct tatgtacgcc tttcctatga gtatctcatc
     6721 ggctatggct attgtcgttt cctatgaagt gggagccaag cgatttgatg atgcgaaaac
     6781 ctatattggt ctaggaagat ggactgccct catttttgcg gccttcacct taaccttcct
     6841 ttacattttt aggggaaatg tggccagtct ttatggtaac gacccaaaat ttatcgattt
     6901 gacagtgcgt tttttaactt atagtctttt cttccagtta gcagatacct ttgcggcgcc
     6961 gcttcaggga attttgcggg ggtataagga tacagttatt cctttttacc ttggtttgct
     7021 tggttattgg ggcgtagcaa tccctgtggc tacgctattt gattctctaa cagattttgg
     7081 agcttattct tactggatcg gcttgattat tagtttgatt gtgagtgggg cgctctaccg
     7141 ttggcgttta actgtgatta tgaagagatt tgaatcttta gcaaaatcca aatgctaaaa
     7201 agtttgtgaa aaaatattct tgacaagaaa atcccttgtt ttcacggggt tttctttttt
     7261 attttgtgaa atttctttta gaaaaacttt gacagtattt gccaaaagcg gtaaaatagt
     7321 aaggtaagaa gaaagttaga aaggcaagaa gatgtcaact ttagataaaa atcttttgct
     7381 agaaatgttc cgtaagatgg aagaaatccg tcgcatggac ttaaaaattg cacaattagt
     7441 aaagaaaggg aaagtgccag gaatgacgca cttttctgtt ggtgaagagg cagctaacgt
     7501 gggggctatg ttagctctca atccagatga tctgattacc tcaaaccacc gtggacacgg
     7561 gcaagctatt gctaaaggga ttgacctcaa cggaatgatg gctgaaatcc ttggaaaata
     7621 cactggaacc tgtaaaggga aaggtggatc tatgcatatc gctgaccttg atgctgggaa
     7681 ccttggtgcc aatggtattg taggtggtgg tatggggatc gctgtcggtg cagccctcag
     7741 tcagcaaatg caaaataccg gtaaaatagt tgtctgcttc tttggagatg gtgcgaccaa
     7801 tgaaggtgtt ttccacgaag cagtgaacat ggcttctatc tggaacctgc cagtcatttt
     7861 ctattgcatt aacaacggtt acggtatctc tgcggatatc aagaaaatga ccaatataga
     7921 acatatccat caacgtagcg ccgcttatgg aattcctgga atgttcatcg aagatggtaa
     7981 caatgtcatc gatgtctatg aaggatttca gaaagctgta gaccatgttc gcagtggcaa
     8041 tggtccagtc ttgattgaaa gtgtaactta tcgctggctc ggtcactcat catctgaccc
     8101 tggtaaatat cgtacgcgtg aagaagtgga attgtggaaa caaaaagatc caatcgaaaa
     8161 cctccgcaat taccttattg aaaataacat tgcaagtgcc gaagaattgg aagaaatcca
     8221 agcgcaagta aaggaagcag tagaagcttc tgttaaattt gcagaggaaa gcccattccc
     8281 accgcttgaa tcagcctttg aagatattta cgcagactaa ggagaaagag ataaaatgga
     8341 aacaaaaaca atgtccttcc gtgacaccat tatccttgct atgtctgagg aaatgcgtcg
     8401 cgatgaaaat gtgttcttga tgggagaaga cgtcggtgtc ttcggaggag acttcggtac
     8461 ttctgttgga atgcttgaag aatttggtcc agaacgtgtc cgtgactgtc cgatttctga
     8521 agctgccatc tctggagctg cagcaggagc agccatgaca ggacttcgtc caatcgtcga
     8581 tatgaccttc atggacttct ctgttattgc catggacaat atcgtcaacc aagctgctaa
     8641 aacacgttac atgtttggtg gtaaaggtca ggttccaatg actgttcgtt gtgcagctgg
     8701 taacggagtt ggctctgccg cccagcactc acaatctcta gagtcttggt ttactcacat
     8761 tccaggactt aaggttgtgg cacctggaac acctgcggac atgaaaggcc tgcttaagtc
     8821 ttctatccgt gataacaacc cagttattat acttgagtac aagtcagaat ttaaccaaaa
     8881 aggggaagtg ccagttgatc cagactacac aattccactt ggggttggtg aaatcaaacg
     8941 ccaaggaacg gatgtaacag ttgttactta tggaaaaatg cttcgccgtg tggttcaagc
     9001 tgctgaagaa ttagcagaag aaggaatttc agttgaaatt gttgacccac gtacccttgt
     9061 tccgcttgat aaggatatca tcataaactc agtgaagaag actggtaagg ttgttctggt
     9121 caacgatgcc cacaaaacaa gtggctatat cggtgaaatt tcagctatta tttcagaatc
     9181 agaagtattt gactatctag atgcaccaat ccgccgttgt gcaggagaag atgtgccgat
     9241 gccttatgca caaaacctag aaaatgcaat gattccaaca gttgaaagta tcaaagatgc
     9301 aatccgaaaa acttataaca aagaatagag ttacataaga taagttatgt aaataaaata
     9361 aagctgacga gaaactttgt atcttttagg tgcagagctc ttcgtcatta atatcttata
     9421 ttcgagcacg ggctaaaagc tcggaaaaaa gataaatctc cttggcttca tcgaagccat
     9481 cgtcgatttc ctatttttca gtcgcttttg acgccctttg tatcataaat agaattggag
     9541 aggtcatggc tgatgacaag ctaagagcga ctcctgcagc tagaaagtta gcggatgatt
     9601 tagggataaa cctctacgac gtttctggct caggtgcaaa cggtcgtgtc cacaaagaag
     9661 acgtggaaac ttataaagac acaaacgtgg ttcgcatttc gccacttgca aaacgaattg
     9721 ccctcgaaca taacattgct tggcaggaaa tccaaggaac cggtcatcgt ggtaaaatca
     9781 tgaagaagga tgttttggcc ctgcttcctg aaaatatcga aaacgatagc atcaaatctc
     9841 ttgctcagat tgaaaaagtg gaagaagtcc ctgataacgt aacaccatat ggtgaaatcg
     9901 agcgtattcc aatgacacca atgcgtaagg ttattgccca acgcatggtt gaatcttact
     9961 tgactgcgcc aaccttcacc ctcaactatg aagttgatat gactgaaatg ttggctcttc
    10021 gtaagaaggt tcttgagcca atcatggaag caactgggaa gaagactact gtaacagacc
    10081 ttctttcgct tgcagttgtt aagactctta tgaaacaccc atacatcaac gcttcattga
    10141 cagaagatgg caagactatt atcactcaca actatgtcaa tcttgccatg gcagttggga
    10201 tggataatgg attgatgaca cctgttgttt acaatgctga gaagatgagt ctttcagaac
    10261 tggttgtagc ctttaaggat gttattggcc gtaccttgga tggtaaattg gctccaagtg
    10321 agttacaaaa ttcaacattc acaatcagta atttgggaat gtttggtgtt cagtcctttg
    10381 gtccgattat taaccaaccc aactcagcta tccttggtgt cagttcgaca atcgagaagc
    10441 cagttgtcgt caatggtgaa attgtgattc gcccaatcat gaatttagga ttaaccattg
    10501 accaccgtgt cgtagatggt atggctggtg ctaagtttat gaaggacttg aaagagttga
    10561 ttgaaacgcc aatctcaatg ttgatttaag agcaagagta ttcattttta agatatagat
    10621 aaaggaagga agacatggcc ttagaagtaa ttatgccaaa agccggcgtg gatatgacag
    10681 aaggacaaat cgtccaatgg aataaaaaag tcggagaatt tgtaaaagaa ggagaaatcc
    10741 ttttggaaat catgactgat aaagtcagca tggaattgga agccgaagaa gatgggtact
    10801 tgattgccat tctcaaagga gatggtgaaa ctgtccctgt aacggaagtt atcggttacc
    10861 ttggtgaaga aagggaaaac atcccaacag ctggagcagc atcgccagaa gctagccctg
    10921 tacctgtagc aagtacctca aacgatgatg gtaagagcga tgatgctttt gatatcgttg
    10981 tgattggtgg aggtcctgct ggttatgttg cagccattaa agctgcccaa ctcggcggta
    11041 aggttgccct tgttgagaaa tctgaacttg gtggaacctg cttaaaccgt ggatgtattc
    11101 caaccaagac ctaccttcat aacgctgaaa ttattgaaaa tatcggtcat gctgcaaatc
    11161 gtggtatcgt gattgaaaat cctaacttca ctgttgatat ggaaaaactt ttagaaacta
    11221 aatctaaagt tgttaatact cttgttggtg gtgttgcagg acttcttcgt agctacggag
    11281 ttactgttca taaaggaatt ggtacaatca ctaaagacaa gaacgtcttg gtaaatggtt
    11341 ctgaattgct tgaaaccaag aaaatcattc ttgctggtgg ttcaaaagtc agcaagatca
    11401 acgtccctgg tatggaatca tcacttgtca tgactagtga tgacattctt gaaatgaacg
    11461 aagtgccaga aagccttgtt atcatcggtg gtggagttgt cggtatcgaa ctcggtcagg
    11521 ccttcatgac atttggttca aaagtgactg ttatcgaaat gatggaccgt atagttccag
    11581 ctatggatgc ggaagtttct aagaaccttc gcttgatcct tgagcgtaaa ggaatgacca
    11641 tcttgactgg tactaaactg caagaaatca ttgaggaaaa tggtcaactt cgtatcaagg
    11701 ttgaaggaaa agacgatatc atcgcaagca aagctcttct ttcaattggt cgtatgccag
    11761 accttgaagg tattggagag gttgagtttg aattggatcg tggttgtatc aaggtcaatg
    11821 aatacatgga aacttcagtt ccaggcattt atgcaccagg tgacatcaac ggtactaaga
    11881 tgttggctca cgcagctttc cgcatgggtg aagtttccgc tgaaaatgcc cttaaaggaa
    11941 atcatgcagt tgccaaattg aatttgactc ctgcagccat ctacactctc cctgaagtag
    12001 cagcagtagg tttgacagaa gaacaagccc gtgagaaata cgatgttgcc atcggtaagt
    12061 ttaactttgc tgctaacggt cgtgctattg catctgacgc agctcaaggt ttcgtaaaag
    12121 ttatcgctga taagaaatac ggagaaatcc ttggtgtaca cattattggt cctgcagccg
    12181 cagaattaat aaacgaagca tcaagcatca tcgaaatgga aatcactgtt gaggaaatgc
    12241 tgaagaccat ccacgggcac ccaacttact ctgaagtgat gtacgaagcg tttgcagatg
    12301 ttctaggaat ggccatccat tcacctaaga aaaaataaaa cgaagataga ttagactgga
    12361 aggttatttt cagtctctat catataaagt aaagggatat catgaaatac attatcaatc
    12421 attcaaatga cactgctttt aatattgcct tggaagaata cgcctttaaa caccttttgg
    12481 atgaggatca aatcttccta ctttggatta acaagccatc tatcattgtt ggtcgtcacc
    12541 agaacactat cgaagaaatc aaccgtgatt atgttcgtga aaatggtatt gaggtagttc
    12601 gccgtattag tggtggtgga gctgtttacc acgatttaaa taacctcaac tacacgatca
    12661 tctccaaaga agatgaaaat aaggcatttg acttcaagag cttctcaact ccggttatca
    12721 ataccctggc tcaacttggg gttaaagctg agttcacagg tcgtaatgac cttgagattg
    12781 atggtaagaa gttctgtggc aatgcccaag cctatatcaa cggccgtatc atgcaccacg
    12841 gttgcttgct ctttgacgtt gatttgtcag tcctcgcaaa tgcccttaag gtttcaaaag
    12901 ataaatttga atcaaaaggt gtgaaatccg tccgtgcccg tgtaaccaat attatcaatg
    12961 aattaccaaa aaaaatcaca gtcgaaaaat tccgtgattt actcctggaa tacatgaaaa
    13021 aagagtaccc agagatgact gaatacgtct tttcagaaga agaattggcc gaaatcaatc
    13081 gcatcaagga tactaagttt ggaacttggg actggaacta tggtaaatca cctgaattta
    13141 acgtccgtcg tggaaccaaa ttcactagtg gtaaggttga agtctttgct aacgttactg
    13201 aatcaaaaat ccaagacatc aagatttatg gtgacttctt tggtattgaa gacgttgctg
    13261 cagtagaagc tgtccttcgt ggggtgaaat acgaacgcga agatgttctt aaggcgctaa
    13321 aaaccattga tatcacacgc tacttcgctg gtattagccg tgaagaaatc gctgaagcgg
    13381 tagttggata aatcaaaaaa agaagttggc caactaacca acttcttttt ttgatattta
    13441 tttacataat ttaaaatacg taaaatcata aactatctag agcattcttt tgttcatcat
    13501 taacaatatg ggtatagagg tcagtgactt gtgtgctggc atgtcctagt tggtgactga
    13561 ctaaaacttg tgatttagtc gcatcataga gcctagttgc tagcgtatgg cgcagtttat
    13621 ggggtgttac acgcacttta aaatcctctg agtatttagc aaccattttc tcaacgctag
    13681 aagcatcgat acgattagga acacctctgt agagagttaa aaaaagggct gtatctgttt
    13741 tttccgtttt atagcgttga ttccgaatgg ccagataatt ctctaaataa ggtttagcaa
    13801 aagcagcgac attgactgag tcacgtttgc caccttttcg agtaacatca ataaccatca
    13861 tttttagatt gagatctctt agatctagat taacagcttc agataagcga acaccagatg
    13921 ccaagagaag ggcaataatg gctaaatctc gttctttatt tttgttgaat gatgagagag
    13981 ctcgatttga aagctgttgt gggtgctctt gatcgatata agttagaaaa ccttctgttt
    14041 catcacctag aaagagtttt tgcttgatat tttcagctct ggcagcaagg gtttctttct
    14101 ttttcttggt tgaaactttt ttcattacat tacgatagaa ataaggttcc ccctgatcgt
    14161 tttcaacctc ctcggttaga tacttgtaaa gactagaaag tgctgataag gttcgattga
    14221 tagttgtctg tgaaacacct tgttttgttg tattagcatt cagcaaggga cgttcacgta
    14281 gataaaggat aaaggattcc atgtctttct tagacatatt ttccaaaact gataaaggaa
    14341 tatcggatat tttatcagcg tttgaaatac cagactccaa aacccagctg aaaaatcggt
    14401 catattcctt aaggtattcg tacagggttg taaaactgta gggcacagcc agcttagatt
    14461 ggtagtattc cagaacatac cagggcatga gttgttttag tttgtcgatt cgttccagta
    14521 aaatctcacg tttcatgtat tttctccata agtaagtata ctagtagtat agcatagact
    14581 tgtatatttt tcaagaaaat tatagttttt cagaaagatg ttataggagt ttttcaaagt
    14641 gataaaaaga cttagaccta caaacaaaac agaataagga aaataatgaa aatctcaccc
    14701 actaaacttc tgttaatact acataactga agagctatat aaggagctat gagatacaaa
    14761 acacattgta taatagattg aaactagaat agtacatctg gacttctaaa acattgctag
    14821 aaatt
//
LOCUS       DAVGJJ010000047        14630 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000047 DAVGJJ010000000
VERSION     DAVGJJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.047"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            391..612
                     /locus_tag="VVE35_001307"
     CDS             391..612
                     /locus_tag="VVE35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061491.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            696..1499
                     /locus_tag="VVE35_001308"
     CDS             696..1499
                     /locus_tag="VVE35_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU3061492.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQTNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDNIEVVKDYDFKS"
     gene            complement(1555..3096)
                     /locus_tag="VVE35_001309"
     CDS             complement(1555..3096)
                     /locus_tag="VVE35_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3061493.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVEDQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKNFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(3098..3580)
                     /locus_tag="VVE35_001310"
     CDS             complement(3098..3580)
                     /locus_tag="VVE35_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU3061494.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(3580..4665)
                     /gene="rlmN"
                     /locus_tag="VVE35_001311"
     CDS             complement(3580..4665)
                     /gene="rlmN"
                     /locus_tag="VVE35_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU3061495.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMTIGARHITVSTLGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(4686..5216)
                     /locus_tag="VVE35_001312"
     CDS             complement(4686..5216)
                     /locus_tag="VVE35_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU3061496.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(5372..5977)
                     /locus_tag="VVE35_001313"
     CDS             complement(5372..5977)
                     /locus_tag="VVE35_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU3061497.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(6150..7187)
                     /locus_tag="VVE35_001314"
     CDS             complement(6150..7187)
                     /locus_tag="VVE35_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU3061498.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDMELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(7221..8156)
                     /locus_tag="VVE35_001315"
     CDS             complement(7221..8156)
                     /locus_tag="VVE35_001315"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU3061499.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(8709..9899)
                     /locus_tag="VVE35_001316"
     CDS             complement(8709..9899)
                     /locus_tag="VVE35_001316"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU3061500.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(10114..11457)
                     /locus_tag="VVE35_001317"
     CDS             complement(10114..11457)
                     /locus_tag="VVE35_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3061501.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(11597..12412)
                     /locus_tag="VVE35_001318"
     CDS             complement(11597..12412)
                     /locus_tag="VVE35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU3061502.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(12464..14617)
                     /locus_tag="VVE35_001319"
     CDS             complement(12464..14617)
                     /locus_tag="VVE35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU3061503.1"
                     /translation="MSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVMINPTFAPLV
                     ITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINRITGTIFGVS
                     GDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGATAYNKYYNF
                     RKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWIANSQETAPI
                     LAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFGQDPLWLAWV
                     TDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNVDADKKHKYK
                     GMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVNLRMHSFGSI
                     EFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPGRNGNYETAE
                     GSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADKVGNAEQWKA
                     EGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEAQQNTVHFKD
                     LTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGTVSSIFPTKH
                     AFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADLDAIRAAGRE
                     TSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT         4348 a         3209 c         2727 g         4346 t
ORIGIN      
        1 taaataactt tcaattaagt gtgaattttg cttaaaatca ttaaaaaaca ccccatggtg
       61 tgaaccatga agtgttgtaa atgctgtgtt gtagctagtt cttagcgttt agagaactgg
      121 ctagctttac gagctttctt aagatctggt ttccaaaata ctttgagtac acttatggta
      181 ttgatgcccc taaacatcta aaaactctgg tagaaaaagg gtatgctctt gtcgaaactt
      241 cttttgactc acttgaccat ctaaataata ccatgaaaaa gaatatcctg aagaagaagg
      301 gagttactgg actttctaaa atgaaatctt gcgacctgga tcaagccctt catgagcatt
      361 tttcagaaga agaattagct ggtcactttc atgtccttct atggactttt ttacaatggc
      421 attgctatca cacccaatac ctatctaagc gcctggttcg taaactttat tgcagctctt
      481 cctctaaatt tcctaattgt tgaaccaatt gcccgtttta tactaagttc ttttcagaaa
      541 ccatttactg gggaagaagt tgaagaagtt gaagattttc aagatgatga tgaaatccca
      601 actattatct aagccagttc tgtaaactac taatatttga aatccacttc cttttagggt
      661 acaatggtta taaatgaatt tttgagagga tcagaatgaa aaaactagca acccttcttt
      721 tactgtctac tgtagcccta gctgggtgta gcagcgtcca acgcagtctg cgtggtgatg
      781 attatgttga ttccagtctt gctgctgaag aaagttccaa agtagctgcc caatctgcca
      841 aggagttaaa cgatgcttta acaaacgaaa acgccaattt cccacaacta tctaaggaag
      901 ttgctgaaga tgaagccgaa gtgattctcc acacaagcca aggtgatatt cgcattaaac
      961 tcttccctaa actcgctcct ctagcggttg aaaatttcct cactcacgcc aaagaaggct
     1021 actataacgg tattaccttc caccgtgtca tcgatggctt tatggtccaa actggagatc
     1081 caaaagggga cggtacaggt ggtcagtcca tctggcatga caaggataag actaaagaca
     1141 aaggaactgg tttcaagaac gagattactc cttatttgta taacatccgt ggtgctcttg
     1201 ctatggctaa tactggtcaa acaaacacca atggcagcca gttcttcatc aaccaaaact
     1261 ctacagatac ctcttctaaa ctccctacaa gcaagtatcc acagaaaatt atcgaagcct
     1321 acaaagaagg tggaaaccct agtctagatg gcaaacaccc agtctttggt caagtgattg
     1381 acggtatgga tgttgtggat aagattgcta aggccgaaaa agatgaaaaa gacaagccaa
     1441 ctactgctat cacaatcgac aacatcgaag tggtgaaaga ctacgatttt aaatcttaaa
     1501 aaccaaaaaa atacagtatc cacattcggt actgtatttc ttttactctc attcttaagt
     1561 taaattatta aaatcccata tttggtctat ccagccttca taaaagtctg gctcgtggca
     1621 gaccataagg atagatcccc tatattcttt gagagcgcgt ttgagctcat cctttgcatc
     1681 cacatccaaa tggttggtcg gctcgtccag cactaaaacg ttgttttcac gattcatcaa
     1741 gagacagaaa cgaaccttgg cttgctctcc ccctgataat acttgaatct ggctttcaat
     1801 atgtttggtt gtcaaaccac aacgggcaag ggctgcacgg acttctgctt gattaagggc
     1861 aggaaaggca ttccagacag cttcaagagg agtttggcga ttaccgcctt ctacttcctg
     1921 ctcaaaataa ccaagttcta aataatctcc acgctccact tccccagcga ttggcgagat
     1981 aatgcccaag agactcttca agagagttgt ttttccaata ccattagcac caataatcgc
     2041 aaccttttga ttgcgttcga aggtaagatt taaaggctta gtaagaggac ggtcgtaacc
     2101 aatttgcaag ttcttggctt ggaagataaa gcgccctggt gtacgagctg gtttgaaatc
     2161 aaaggatggt tttggtttct cactttggag ttcgataata tccatcttat ccaatttctt
     2221 ttgacgagac atagccatat tacgagttgc aacacgggct ttattacgag ccacaaagtt
     2281 cttgaggtct gcaatctctt tctgctggcg ttcgtaggct gcctctagct gagatttctt
     2341 catagcataa acttcttgga actggtagta gtcaccagag taacgcgtca gctgttgatc
     2401 ttccacatga tagacaatat taataacgtc attgaggaat ggaatatcgt gcgaaatgag
     2461 aacaaaggca ttctcatagt tttggagata gcgcttgagc caatcaatat gctcagcatc
     2521 caagtagttg gtcggctcgt ccaacagcaa gatatcaggc ttttcaagga gaagttttgc
     2581 caaaagcacc ttggttcttt gcccacctga caaagaagtt acatccgtat ccatgccaaa
     2641 gtccataaca ccaagagcac gcgctacttc gtcaatctta gcatccaagg tatagaaatc
     2701 acgactctcc agacggtctt gaagttctcc tacttcttcc atgagagcat caacatccgc
     2761 gccgtcttca gccattttca tatagaggtc attgatacga gcttcagctt tgaaaagctc
     2821 atcaaaagcc gtacggagaa catcacgcac cgactgtctt tcagcaagga cagagtgctg
     2881 atccaagtaa ccagccgtca catatttgga ccactcaacc tttccttcat ctggcagcat
     2941 tttaccagtc acgatactca taaaggttga ttttccttca ccattggcac cgaccaggcc
     3001 gatatgttct cccttgagga gacggaagga cacatcttca aaaattgcac ggtcaccaaa
     3061 accgtgactc agatttttaa cttctaaaat actcatttta attccttatc ttgtttttat
     3121 gtaatcgttt ataaaggagc caagccagat agccacccaa agtgttggtc cacaaatcat
     3181 caatctcaaa gacgcgattg aaatcaaaga aaaagtccaa gattaattgc gtacactcga
     3241 ttccaagact cacaagaaaa ctaaaaagaa ggaccttttt tgttttccgc aaatttggaa
     3301 atagataaag gagttggaaa atcagaggaa aaaacaagaa gacattgagg acattttgta
     3361 aaaaaatcca acataattgt ccaatgtcac tcacttcgcc cagtttccag agagaattga
     3421 aaggagtcaa aagaaaaacc aggcgtccaa gatgctgaat acctggagtt cccactccca
     3481 cggtagattg ttcttgagga gtaaagcaaa aacagacaat gcaaatgcta tagaaaatga
     3541 ctccccagac caaaatatga ttataagtct tcttcatcat taaggattta ccgctgcgac
     3601 tgccttctgg cggtcacgtt tcattgtgtt agagcgcaat tgtccacaag ctgcgtcaat
     3661 atctgtacca tgctcttgac gaaccacaca gttgacccct tttttcttaa gcgtatcata
     3721 gaaagccaac acgcgctctt tgggactacg gctatattgg tcatgctcac taactgggtt
     3781 ataaggaatc aagtttacat aagacaattt cttgatgttc ttgagcaatt cagtcaattc
     3841 caaggcttgt tctacactgt cgttgacttc attaagcatg atatattcaa aggttacacg
     3901 acggtttgtt gtctcaatgt agtattcaat agcagcaaag agtttttcaa tcggaaaggc
     3961 tcggttaatc ttcatgatac ttgaacgaag ttcattgtta ggtgcgtgaa gagacacggc
     4021 aagattgacc tgaacccctt catcagcaaa gtcacgaatt ttatgagcca aacctaaggt
     4081 tgaaaccgtg atgtgacgag caccgatagt cattccttta tcatcattga tagtacaaac
     4141 gaaattcaag acattgttgt aattatcaaa gggctcaccg attcccatga caacgatatg
     4201 gctgacgcgt tcatcctgac cacgctcagc aaagtatttc tgaaccagca tgatttgcgc
     4261 tacgatttca ccgttattga ggtcacgttg cttcttaatc aaaccagagg cacagaaggt
     4321 acaaccgata ttacagccga cctgagtggt cacacagaca gataaaccat agtgttgacg
     4381 catgagtaca gtctcaatta acataccgtc gggcaattca aagagatatt tgactgtacc
     4441 atcagcagac tcttgcacaa tacgttgttt caagggattg accacaaact ggtcattgag
     4501 cttagcaatc aaatccttgg aaaggttggt catttcttca aatgactgca cacgtttacg
     4561 gtagagccat tcccagattt gatctgcacg gaatttcttt tctccctgct ccaataccca
     4621 ttcctgcatg gtttgatgtg ccaaactatg aattgagggt ttcatttctt ctccttattc
     4681 tctactcact tctgacgaat gacaaaatga cgttgtccct tgtcctcttt ctgagaatgc
     4741 tggtctttct gacgacgtct atttttctta tctgcattcg actttcgttt agtttgagtc
     4801 ggtttctttc cttttctaga aggtgtttct tcttccgtct tacgcatttt cttgtcaaat
     4861 gatgctcgct taggggcttc attttctaag acaaaatagg cacaaccata actacaatac
     4921 tctaaaaggt agtcttgtaa acgactgatt ttttcaagtt tttcttctgt tcggtcatcc
     4981 ttgtaaaaac ctcgtaggcg aagctgttcg ttgctccagt cccccacgat ataatcaaac
     5041 ttggttaata cttctgaaaa acgctgatta aaagtcgtca catcaaaggc atccttgata
     5101 ttttcaacca aggaaaaagc tatcccttcc gtttcgacct tgtccccgtg taaatggaac
     5161 tccggaccag gaaacttgtt atagttgtat aattcaggtg caatttcttt tcgcatagat
     5221 atcctttttt cacgattact taatacttta ttctaccata atttctagca gttagcacgt
     5281 ttctcataaa aatgaaaaaa gtctgacgat tttgtcagac cagaatctta taacctaaaa
     5341 agagaagaac aattcttccc tccaactatc attatttagc agctgcgtac aattcatcta
     5401 ctttattcca gttgattact gaaaagaaag ctttgatgta gtcaggacgc acgttgcggt
     5461 atttcacgta gtaagcatgt tcccaaacgt ccaagcccaa gattggtttt ttaccttctg
     5521 agattggtgt gtcttggttt gctgttgaag tcacttcaag tttcccttct ttgttgacaa
     5581 ccaaccatgc ccaacctgaa ccaaaacgag ttgttgctgc tgcagtgaag gctgcttgga
     5641 attcttcaaa tgaaccaaat gttgcatcga ttgctgctgc cagttctgct gaaggagctg
     5701 ttttctcggg agtcatcaat tcccagaaaa gagcgtggtt caagtgtccg ccaccattgt
     5761 tgataagtgc ttgacggata tcagctggga tagattctac atcagcaagc aaggcttcaa
     5821 ggtcttcacc gatttcaggg tgtttttcta aagctgcatt ggcattgttg acataagttt
     5881 gatggtgttt gtcatggtgc aaatgcattg tttccgcatc gatgtatggt tccaaagcgt
     5941 cgtatgcata tggaagttct ggtaagataa tagccatctg taatacctct ttttctttct
     6001 atatgaaaat gataacgcaa acattcactt ttttcaagtt ttttgcttat agataaggaa
     6061 atcactgaca tactttctaa taaaataagc aaatcagcaa gaaacccacg acaagatagt
     6121 cctgacgcgg gttgtagttt caacaaatgt caattgacct gactagcaat ctgtaagagt
     6181 gccttttcaa aaaggaaacc tttttcataa agacctgtct taatctgata gtctgtctca
     6241 atcaaatagg aaatagcttg cttcaaaaag ctcaaagaaa gtcctctcga atctcttaat
     6301 gcaaacttga tttgataagg atttgggtta cgtcccagat aactacctaa actacttgca
     6361 atctgcgatt ctgtttggcc agactccgcc aaaatcttca cctgagtaaa agtccgaaat
     6421 tgtcccagca tgactgcaat cagtttgatt tcatcttccc cttgcaaggt caagtctctc
     6481 accaaatcgc gcgcctgatc catcttttta gtcagaataa actgagttaa atcaaaaata
     6541 ttgtcctgca aggtcttggg aattgcgtta acaatatcct cttcctcaat aacagaattc
     6601 gccttatagg actgtaaaaa gagaagattt ttctggattt cgctaaattg aaaccccgac
     6661 ttgatgagga gattttcaaa agaatgattg gtaaactgca gaccttgttt ctgactccac
     6721 ttttggaaat actggcgcaa ttcttgttct tttacttcta ctgcatcgaa gaccttggca
     6781 tcacgcttaa gtaatttgac taaccgtctt ttgctatcca gctttccttc tgcaaagatt
     6841 atcaacttgg ttgttggaga agaattgtca aggtattcct caaatgactt aagctcatca
     6901 tctgtcaaaa agcgtttctt agcagtcgtg atatccataa aataatctaa tatcacgatt
     6961 ttctcatccg caaagaaagg aaggctgacc aactccagtt ccatatcctt gtaaactact
     7021 tctttcatat caaagtaggc aaagttgagg tcagcagaat catacccaat ctgtttcaac
     7081 acttgactct tcatcacttc aaactgaccc tgatctgtcc ctgtaaatag gctcaggctc
     7141 ggtaaatttg ataaagtcaa cttctgactt tcttcaatgg ctagcatcgt ctctcctttc
     7201 ttcagatttt tcgatttaat ttagtcaata tagcgcaatt tcccacggaa atcttctaag
     7261 ctctcgtagc ctttttccac catgattgct ttcagttcat tggtaatgcg gtcaaaagca
     7321 ctgacgcctt ctttgtgaag ggtcgttccc acctgcacca tacttgctcc acagaggatg
     7381 tgttcaaagg catctcgacc agtcagaacg ccacctgttc cgataatttg gatttgagga
     7441 tttaaacgtt gataaaaggc gtgaacattg gctagagcag tcggtttgat gtattctcca
     7501 ccaattccac caaaaccatt cttaggccga ataacgacag attcgtcttc tatatagagg
     7561 ccgtttccga tagagttaac gcagttgaca aacttgagcg gatatttgtt gaaaatagct
     7621 gccgcttggt caaagtgaac aatatcaaaa taaggtggca atttaattcc aagaggtttg
     7681 gtgaagtaag caaacacttc tgccaaaatc cggtctgttg tctcaaaatc ataggcaatc
     7741 tgaggtttac ctggaacatt tggacaggaa agatttagct cagtcaaacc acgaaaatca
     7801 ctctcttgga cttttttcaa aatagtatgg gtttcctctg gagacatgcc gaccagagat
     7861 aagaagaaag ttcggttcga ctctttttcc tgcaaatcta aaagataatc caaataatag
     7921 tctaagccat tatttggcaa gcccatagag ttgatggaac caagtggaac atcttggtag
     7981 cgtggctcag gattcccctg acggaagtcc aaggtcgctg tcttagtaac aaaggttcct
     8041 gccgctgagt ttttgacctc ttctaactcc tctatcgtca tacaagccac acctgctgca
     8101 ttcatcaagc aattgtcaaa ctcaaaacca gcaatttgtg ttttcgttga taccatgatt
     8161 ctaatcctcc agatttcttt tattgctaat attataccat aattttaatt ctaggtttac
     8221 tagattctgt ggttctatct atttattcgg aaaagaaagt ctagtacaga agtcatgcta
     8281 tggaaacagc tagtgtattg gaagtttgaa caaggcttgg gtaacagact tttgaatgaa
     8341 gtccaagcaa caaggagaaa tctcaaccat gagtccattc catgactctg ttgctctaga
     8401 aaaccttgca atagtcaagt gataacgaat aaaactttgg attgaaatat ccccatgatt
     8461 atctattacg aggagaaatc tcaaccttta tcatttttaa agaaatatag taaaatgaaa
     8521 caagaacagg agaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagtt
     8581 gaagtgtact attctagttt caatctacta tacatgtaaa tctctacttt cgcaaaatgg
     8641 attttcagaa cagtatccca atatagttaa aaactataaa aaagagagga cgttcccctc
     8701 ttaaaatctt attttactgc ttctttcaaa gcatctacct tatccaaacg ttcccatgga
     8761 agatcaatat ctgtacgtcc catgtgaccg taagccgatg tttgacggta aattggacgc
     8821 ttaaggtcca gcatttggat aatccctgca gggcgaaggt caaagatttg acgagccgct
     8881 ttttcaagtt gactttcagc tactgttcct gtaccgaaag tatcgatacg aacagaaaca
     8941 ggttgcgcaa caccgatagc ataggccaac tgcacttctg ccttcttagc aaggcctgct
     9001 gcaacgatat tcttggcaat atagcgagcc gcataagagg ctgaacgatc caccttagtc
     9061 gcatctttac cagagaaggc accaccacca tgacgagagt agccaccata agtatctaca
     9121 ataatcttac gaccagtcaa acctgagtcc ccttgaggac caccgattac aaaacgacct
     9181 gtcggattga taaagaattt tgtcttatca tcaagataag aagatggaat aacttctttg
     9241 atgaccttgt caatcacatc ttgatggatt tgttcattag tggcctctgg atcatgctga
     9301 gtagaaataa cgactgtatc tacacgtacc ggacggtcat tttcatcgta ctcaactgta
     9361 acttgtgatt ttgcatctgg acggagatag ctaatttctc cagacttacg aagttctgcc
     9421 agacgacgaa ccaatttatg actgagtgca attggcaatg gcatgagctc ttctgtttca
     9481 tctactgcaa atccaaacat gagcccttgg tcacctgctc caatcaagtc cagtggatct
     9541 tgatcagcat ttccacgaac ctccaaggct tcgttaacac cttgagcgat gtcaggagat
     9601 tgttccacca aagatgggtg tactcccacc gtctcagcag aaaatccata ttctgtattg
     9661 gtataaccaa tctctgcaat ggtatcacga accacacggt taatatccac ataggcattt
     9721 gtagaaattt caccaaaaac gtggacagaa ccagtatata cagctgtttc agcagcaacg
     9781 tgcgcctctg gatcctttgc taaaatagca tccaaaatcg catctgaaat ttggtctgca
     9841 atcttatccg gatgcccctc agatacagat tcagacgtga ataatttacg ttctgacata
     9901 aaaatgtccc cccttaaaaa atattgttat agagttacaa agtactgata ggaaaactct
     9961 tcgtaataaa tacctaccat cttatcggga ctatataact ttatcattat accattttta
    10021 atcgcaattt gcagtcttta atgtagtgtt tcggtttaga tagttcgttc tcataaataa
    10081 aaaagttgga gctcagtact ccaacaattt ctattaaaat gtttggcgtt tagctttacg
    10141 ctctgcacga ccgcgagcgc gattttcagc acgcttggtt ttacggcgct tttcatcaac
    10201 cgcccattga attttcttct tataacccgg tttgactttt ttctttttct ttttaaccaa
    10261 accaatcatt tcgatatcaa gtttatcttg ttttttctca cggttggcac gacgatcacg
    10321 gtcataggta tcttgaaatt ccccgtcttt gaccatctta ggactaaact tgattcccaa
    10381 tttctccaac tcacggatat ccgagtcatc acttggctga taaagggtaa tagctgtacc
    10441 tggtaggcca tttcgtccag tacgaccaac acggtgaaca aaaaaagata agtcttgcgg
    10501 aatggcatca ttgatgacat ggctgacacc ttcaatgtca atcccacgcg ctgctaaatc
    10561 tgttgcgaca atatactcaa aatccagatt ttgcacctga ttcatgattc gcttgcgttc
    10621 acgaggggca atatcgccat ggatttttgc aaccttcaag ccttgagcag tcagatatga
    10681 atgcaattca tcagcacgcg ttttagtgtt aacaaaaatc attgccaaat acggctgcat
    10741 caactgagtc aactggtaaa tttgagcatt cttatcatgt cccttggtcg aaatcaacca
    10801 attatcaatg gtgtcagaaa taaccgtttt agtcttaatt ttctccataa caggatttga
    10861 taagtatttt ttcaagaatg gttgcagttt ttgtgggata gtcgctgaga agaccatgaa
    10921 ttgcaagtct tttggaagac tgccagcaat cttatcaaca gtttccaaga atcccatatc
    10981 caaggtcata tctgcttcat caacaacaaa tgtcttggct ttatgaatag ctaaatcacc
    11041 agatttaacc aagtcgtaga tacggcctgg tgttccaata acaatatgag gctgattgct
    11101 ttccaatttc tcaatctggc gagccttatc cgtaccaccc acataattaa ccacacgaac
    11161 ttcgacatct gagtgagctg aaatctgacg cgctacttgg taaatttgag tagccaactc
    11221 acgactcggt gcagtaatca ctgcttgtac actatcgcta gcttcatcta attgctggaa
    11281 aatcggtaac aagaaagtgt gagtcttacc tgaacctgtt tttgattctc ctactaggtc
    11341 acgacctgcc aaaacaatag gaatcaactt gtcttgcacc tctgttggag ttgtaaattt
    11401 taactccttc aaggcttctc taatatagtt tttaaattga aatttcgtaa atgacataac
    11461 atcctcgatt ctatctatct tatcaattat accatatttt attccattac agtagtctca
    11521 cttatttagg ctatttccag tagcttctct agtaagaaaa ggctggaatt ttatagttcc
    11581 aacctctttt cagttattat ttccagttta acatagcatt caagccatag tgatcactca
    11641 cttgtggact cttgttacca tcaaatacga catgtaaatt ttccaccgct aactctttgg
    11701 tagtaaagac ataatcgatt cgaaggggtt cagtgttccc tttccagcca tcaatttcag
    11761 gcggaacagt atagctacca cttttctctt gagcaacttc aaatgcgtct tgtaagccta
    11821 atggactagc taaaatagct tggtaacctt cctgacctgc tgggttgtta aaatctccag
    11881 ctagtaaaag tggcttgttc aattttttca agacagcctc aaatcgtgcc cattcttctt
    11941 ggaaaccttt atcccaccaa gagagatgaa cactggcaac tgctagctcc ttgccatcga
    12001 ctacagtttc agctagggca acacggcgag tatgatagtc tgttggatca tccacatctg
    12061 aaaccaaaat ttctctggct tcaataggtg ttttagacaa gatagccaca ccttcgtggt
    12121 agcggtcata gccgatatgg ttataagccc aggtccagta gtaatttttc ccttgctcta
    12181 acaacttttc aaccaagagt ctaacataat ggtcttggtg aataggctca gctgctggca
    12241 aagcttgata aaggtcatta acctccacct ctgacgaggt catctcctga ttaatttctt
    12301 ggaaacaaat caaatcatag tccttttcaa gaatatcttc aagcaaaatc tggaattttt
    12361 cctctgcttc tttctccatc caactatgag tattgagtgt taaaaatttc attcttttct
    12421 cccaaaataa gaggttggaa tacagcttcc aacctcaagt atattacaat tctactttag
    12481 caactgctgt tttagctgca agagaacctg ttttttctaa tttaactgat ttaattgcat
    12541 caccatttgt gaagacaact actgttgaag tttcacgtcc tgctgcacgg atagcatcca
    12601 agtcagctgt gacaaggaga tctcctgctg caactttttg tccttcagca acatgaactg
    12661 taaatggttt accttcaaga cttactgtgt ccaaaccaat gtgaaccaat acttcaagac
    12721 ctgcttccgt cacaatacca aaagcatgtt ttgttgggaa gatgcttgac acagtacctg
    12781 aaactggaga tacaatgttt ccatttgcag gttctactgc aaatccatca cccatcattt
    12841 tttgagcaaa tactggatcc tttacttgtt ccaaaccaac aacttgaccg tctgctactg
    12901 aataaacttc ctcagtaaga tctttgaagt gaacagtgtt ttgttgtgct tcagtcattt
    12961 ggcttggaag agtttcagga atgatttcac ctgaatcaag gatatcttgg atatcagatt
    13021 tcaaaatgtc agcttttgga ccgtagatag cttgaacccc ttgtcctttc atcacaagac
    13081 ccatagctcc ttctgctttc cattgctctg catttcctac tttatctgca tctttaacag
    13141 ttacacgaag acgagtcata catgcatcaa catcaacgat gtttacacgt ccaccaagaa
    13201 ggttgataat gtttacagct tgagagcctg ctgcaacttt cacttcgctg ctggtttctt
    13261 ctgaaccttc agcagtttcg tagtttccgt tgcgccctgg agttgcgtaa ttgaattttt
    13321 gaatcatgaa gtttgcgata aagtacatga ttacagcaaa gagaacagtt acccaaacga
    13381 agttaacgat atccatacca ataccagcac tgattgcaat aggtgtacga gtcaagaact
    13441 cgattgaacc gaatgagtgc atacgtaggt ttacgacgtc agccatagcg aaggcagcac
    13501 cttgaacaag tgagtaaaca agatacatag gtgttgcgat gaacatgaac atgtattcga
    13561 ttggttcagt aacccctgtc aagaatgttg caagagctgt tgcaatcatc atacctttgt
    13621 atttatgttt cttgtcagca tcaacattac ggtagatagc aacaatcaca cccatcaaga
    13681 taccgaatga accgatcatt tgtccaactt tgaaacgagc tggatgtact gtatctaaca
    13741 agtgttgata ttgactagca tcagtacctt taaggtttac aaggtctgtt acccatgcaa
    13801 gccatagtgg gtcttgaccg aatacttgag tacctttagc tgcaccagtt aaaatgtcat
    13861 aagtaccacc aagagctgtg tagttcattg ggatagtcaa catgtggtga agaccaaatg
    13921 gcaagagcaa acgttccaaa gtaccataca agaatggtgc aagaattgga gcagtttctt
    13981 gtgagttggc aatccagata ccgaagttat tgatacctgt ttgaactact ggccagaaag
    14041 cagcaagtag aattgcagcg attgctgaac gaagaataac tacaaatggt acgaaacgtt
    14101 tcccgttgaa gaatgaaagt gcatcaggaa gtttacggaa gttgtagtat ttgttgtaag
    14161 cagttgcccc tacaaaacct gagataatcc ctacgaatac ccccatgttc aaggctggag
    14221 cttcaagaac actgataaag taatcagcaa ctttgattga accaccaaag aaagtagtta
    14281 ccatagcatc tggatttttc aacatatcgc ctgatacacc aaagattgta ccagtgatac
    14341 ggttaatcaa gatgaaggca agaccagcgg cgaaagcacc accagcacgt tctttagccc
    14401 agcttcctcc aatggctagg gcaaacaaaa tgtgaaggtt accgataacc ccccaaccga
    14461 tttgctcaag aattccacct gtgatgacaa gtggtgcaaa ggttgggtta atcatcacga
    14521 tagacttacc gattgaaatc atcaaaccag cagccggcat aaccgcgata actaccatca
    14581 aagccttacc gaatttttgc caaaattcga aagacaagac atttttgaat
//
LOCUS       DAVGJJ010000048        14582 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000048 DAVGJJ010000000
VERSION     DAVGJJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.048"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            32..895
                     /locus_tag="VVE35_001320"
     CDS             32..895
                     /locus_tag="VVE35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061504.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1278..1898
                     /locus_tag="VVE35_001321"
     CDS             1278..1898
                     /locus_tag="VVE35_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061505.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFVAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1984..2427
                     /gene="tsaE"
                     /locus_tag="VVE35_001322"
     CDS             1984..2427
                     /gene="tsaE"
                     /locus_tag="VVE35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU3061506.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2417..2935
                     /locus_tag="VVE35_001323"
     CDS             2417..2935
                     /locus_tag="VVE35_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061507.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2943..3959
                     /locus_tag="VVE35_001324"
     CDS             2943..3959
                     /locus_tag="VVE35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU3061508.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4043..5299
                     /locus_tag="VVE35_001325"
     CDS             4043..5299
                     /locus_tag="VVE35_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU3061509.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5354..6520
                     /gene="recA"
                     /locus_tag="VVE35_001326"
     CDS             5354..6520
                     /gene="recA"
                     /locus_tag="VVE35_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU3061510.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6831..8201
                     /locus_tag="VVE35_001327"
     CDS             6831..8201
                     /locus_tag="VVE35_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU3061511.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAIGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8577..9533
                     /gene="lytA"
                     /locus_tag="VVE35_001328"
     CDS             8577..9533
                     /gene="lytA"
                     /locus_tag="VVE35_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU3061512.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            9892..10053
                     /locus_tag="VVE35_001329"
     CDS             9892..10053
                     /locus_tag="VVE35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061513.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            10040..10246
                     /locus_tag="VVE35_001330"
     CDS             10040..10246
                     /locus_tag="VVE35_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061514.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            10292..10651
                     /locus_tag="VVE35_001331"
     CDS             10292..10651
                     /locus_tag="VVE35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061515.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKETVLG"
     gene            10780..11205
                     /locus_tag="VVE35_001332"
     CDS             10780..11205
                     /locus_tag="VVE35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061516.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILESNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            11614..12408
                     /locus_tag="VVE35_001333"
     CDS             11614..12408
                     /locus_tag="VVE35_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061517.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            12412..12609
                     /locus_tag="VVE35_001334"
     CDS             12412..12609
                     /locus_tag="VVE35_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061518.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            12599..13150
                     /locus_tag="VVE35_001335"
     CDS             12599..13150
                     /locus_tag="VVE35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061519.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            13209..13469
                     /locus_tag="VVE35_001336"
     CDS             13209..13469
                     /locus_tag="VVE35_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061520.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(14233..14517)
                     /locus_tag="VVE35_001337"
                     /pseudo
     CDS             complement(14233..14517)
                     /locus_tag="VVE35_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4602 a         2350 c         3117 g         4513 t
ORIGIN      
        1 attaagcaaa taggaaggtt tattatcttt aatgaataca ctcgcagaga aattcagatt
       61 gaaaagaaaa gagttgagac tctcccaaca aactcttgca gaaggaattt gtgaacaaag
      121 tcagattagt aaaattgaga gagggcattt cattccctcc gcagaccttt tgttcaaact
      181 ctctcaacga cttgaagtac cattagatta tttttttaat gaacaaattg aaattaaatc
      241 taacctctct aatttcaagc aattgtctgc tcgactatta gacgatagaa attatgacga
      301 tttggaatat atttatagaa tagagattga acgaagtact tttctaacac tagaagaccg
      361 aacttacctt gaatggatta aagctattat tgacttctat caatatgaca gtaagtgtga
      421 ggcaatttct tcattggaaa atatattatt aaaagtctct tcaaatactc tgatttattt
      481 aaaggcgttg aatactttat ctaatttcta ttccttagtg ggtcgtgaac aagaatatga
      541 ggcaaactac tctcatttaa tagagttgta tcagacaaaa aatttggacc atcaagagtt
      601 tttatttggc tacatcagag ttcgttacaa ctacgctcac tacctagtat caaaggaaaa
      661 atataatgaa gccatccaag aagctcttga aacgattgaa ctctgtaaac aaagacagac
      721 aagctaccaa ttggctcctc tacttattct tgtaggaaat gctggagcca aatttctaga
      781 cagagaacaa gtcaaaaatt attatataga agcaagagag ttatgtaaga tttataacaa
      841 tcctttaatg ttgatgaaga tagaaaatta tttgaaggaa ctagatactg tttagttaat
      901 cttaacatta tagtttttct gaaacgttaa ataacctgta aggctgatag ttagattaat
      961 actatcagcc ttataattat gtgactctga gtccgtctcg ctccgtgagg tgcgagacaa
     1021 aacaaccacc cgtttgacgg attttcatga tttcaaattt atattcttcg aaaatctctt
     1081 caaaccacgt cagctctatc tgcaatctca aaacactgtt ttgagcaacc tgtggctagc
     1141 tacctagttt gatctttgat tttcattgag tattagtctc tagtgtttgt ttagcgtctt
     1201 aaagagacat ttgagaagat tagaaacagt tctctctttt ctcctagtat aatgattggt
     1261 aggaagggag agagaagatg aaatcaatga gaatcttatt tttgttagct ttaattcaaa
     1321 tcagtttgag tagctgtttc ctatggaagg aatgcatctt gtcctttaaa caaagtacag
     1381 cttttttcat cggaagcatg gttttcgttt caggaatctg tgctggagta aattatcttt
     1441 atacccgtaa gcaagaagtc catagtgtcc tagccagtaa gaagtcggtg aagctttttt
     1501 acagtatgtt actcttaatt aatttgttag gagctgttct tgttttgtca gataacttgt
     1561 tcatcaaaaa tacgctgcag caagaattag ttgacttttt attgccatcc ttctttttcc
     1621 tatttgggct agatttgctg atttttttac ccttgaaaaa atacgtgcgc gattttcttg
     1681 ctatgctaga cagaaaaaag acagtgttgg tgactatttt agcaacactt cttttcttaa
     1741 gaaatccaat gaccattgtc tcacttctga tttatactgg actgggcttg tttgttgcag
     1801 cctatcttgt cccaaattcg gttaagaagg aagtttcctt ttatggtcat attttccgag
     1861 atcttgtatt ggtcattgtt acgctcattt tcttttagag aaagcctgtt ttttatggat
     1921 gttatgatga taagtttagt aggaaaatag acacaaaaga aaagtttttg gtataataag
     1981 agtatgtaca caaaaaatga agaagagttg caagccttag gggagcgttt gggccatcta
     2041 ttagcaaaga atgatgtttt aatcttaatt ggagaactgg gtgcaggtaa aacgaccttt
     2101 actaaaggac ttgcaaaagg attacagatt tctcaaatga ttaaaagtcc tacctatact
     2161 atcgtgagag agtatgaagg tcgacttcca ctttatcacc tagatgttta tcgtattgaa
     2221 ggagatgctg attctatcga cttggatgag tttatctttg gtggcggcgt gactgttatt
     2281 gagtggggaa atctcttagg agatgccttg ccagatgctt atttagaatt ggaaattcta
     2341 aaagaagcag atggacgccg tttaaatttt caggcaaagg gtttgcgtgc tgagaaattg
     2401 ttagaggagc ttcaatatgg agtatgaatt gctcattagg gaagcagagc ccaaagatgc
     2461 agctgaatta gtggcctttt taaatcgtgt gagtttggag acagacttta ccagcctaga
     2521 cggagatggt attctcttga ctagtgagga gatggaaata ttcctcaaca agcaagctag
     2581 ttcggacaat cagataacct tacttgcatt cttaaatggc aaaattgctg gtattgtaaa
     2641 tattacagct gaccagcgta agagagtccg tcatattgga gatctcttca ttgtgattgg
     2701 aaaaagatat tggaataatg gcttgggaag tttgttgcta gaagaagcga tagagtgggc
     2761 acaagcaagt ggcattctgc gtcgtctcca actgactgtc caaactcgta atcaagcagc
     2821 agtccatctt tatcaaaagc atggctttgt cattgaaggt agccaagagc gtggggcata
     2881 tatagaagaa gggaaattta tcgatgttta cttgatgggt aaactgatag attagtagaa
     2941 atatggttaa aaaaattatt ggaatggtgc tagctttact ttctgtaact gtagtaggag
     3001 taggtgtttt tgcttatact atttatcaac aagggacaga aaccttagct aaaacctata
     3061 aaaaaatcgg cgaagaaacc aaggttattg aagcgactga acctctaacc attctgttaa
     3121 tgggagtgga caccggaaat gctgaacgaa ctgaaacttg ggtcggtaga agtgatagca
     3181 tgatcttgat gacagtgaat cctaaaacga aaaaaacaac aatgatgagt ttagagcggg
     3241 atattctgac gcgcattgaa tcagggaatg gtcaggctca tgaagcgaaa ctgaactcag
     3301 catatgcaga tggtggagca gagcttgcta tagaaaccat tcaaaaaatg atgaatatcc
     3361 atattgatcg ctatgtgatg gtcaatatga gagggttgca aaaactagtg gatgcagtag
     3421 gaggtattac agtcaataat atcctaggtt tcccaatttc tatcagtgac caagaagaat
     3481 ttaatactat ttctatcggt gttggggagc aacatattgg gggagaagaa gccctagtct
     3541 atgcacgaat gcgttaccaa gatcctgagg gggattatgg tcgtcaaaaa cgtcaacgtg
     3601 aagttattca aaaagtcatg gaaaaagctc tcagtttaaa tagcgttggt cattatcaag
     3661 agattctaaa agctttgagt gacaatatgc agaccaatat tgatttgtct gcaaaaagta
     3721 tccctaactt gctaggctat aaagattcat ttaaaaccat tgaaactcag cagttgcagg
     3781 gtgaaggaga gatacttcaa ggtgtttctt accagattgt ttcgagagca catatgttgg
     3841 aaatgcaaaa tatactccga cgttctttgg gacaagaaga agttactcag cttgaaacca
     3901 atgcggtttt atttgaagat ttatttggca gagcacctgt tggtgatgaa gataattaac
     3961 ttttgataca aataaaaaat caatcgtagg aaaattcctg cgattttttc aaaaaaatac
     4021 gaatagatag gtaggaggaa acatgaaagc agaaatcatt gctgttggaa cagagatttt
     4081 gacaggacag attgtcaaca ccaatgccca gtttttgtcg gaaaaactag ctgagattgg
     4141 ggtagatgta tattttcaga cggctgtagg agacaatgaa gttcgtctct tgtctttgct
     4201 tgagattgcc agtcaacgta gcagtctggt gattttgaca ggcggtttgg ggccaactga
     4261 ggacgaccta accaaacaaa ccctagctaa atttttaggg aaagcattag tctttgatcc
     4321 tcaggctcag gagaagttgg atatcttttt tgccctgcga ccagactatg cccgaacacc
     4381 gaataacgaa agacaagctc aaattgtaga aggagcgatt ccactgccaa acgaaacagg
     4441 actggctgtt ggaggaaaat tagaagtaga cggagtgacc tatgtcgtcc ttccaggtcc
     4501 gccaagtgaa ttgaaaccca tggtcttaaa ccaacttcta cccaagttga tgacagggag
     4561 caagctgtat tcccgagttc ttcgtttctt tgggattggc gagagccagt tggttacgat
     4621 tttggctgat ttaattgata atcagatcga tcctaccttg gccccttatg ccaagacagg
     4681 agaagtcact ctacgtctgt caacaaaggc tagcagtcaa gaagaggcga atcaagcgct
     4741 ggatatcttg gaaaatcaaa tcttggactg ccagactttc gaaggaattt ctttacgaga
     4801 cttttgctat ggttatgggg aagaaactag tttagccagc attgtggtag aagaactgaa
     4861 aaggcaaggg aaaaccatcg cggctgcaga gagtttgacg gcaggtcttt tccaagctac
     4921 cgtggcgaat ttttctggag tttcaagtat atttaagggt ggttttgtga cctatagctt
     4981 ggaggaaaaa tcaaggatgt tggatattcc tgccaagaat ttggaagaac atggtgtggt
     5041 gtctgaattt acagctcaga agatggctga gcaggcacga agcaagaccc agtctgattt
     5101 tggaattagt ttgactggag tggcaggacc agatagccta gaaggacacc cagttgggac
     5161 agtcttcata ggcttggcgc aagatcaagg aactgaggtt atcaaggtta atattggagg
     5221 cagaagccga gcagatgtac gtcacattgc ggttatgcat gcctttaacc tagttcgcaa
     5281 ggctttatta agtgactaac ttttgatata atagtagata ggtctgagga tcattagaat
     5341 gtaggagaat agaatggcga aaaaaccaaa aaaattagaa gaaatttcaa aaaaatttgg
     5401 ggcagaacgt gaaaaggcct tgaatgacgc tcttaaattg attgagaaag actttggtaa
     5461 aggatcaatc atgcgtttgg gtgaacgtgc ggagcaaaag gtgcaagtga tgagctcagg
     5521 ttctttagct cttgacattg cccttggctc aggtggttat cctaagggac gtatcatcga
     5581 aatctatggc ccagagtcat ctggtaagac aacggttgcc cttcatgcag ttgcacaagc
     5641 gcaaaaagaa ggtgggattg ctgcctttat cgatgcggaa catgcccttg atccagctta
     5701 tgctgcggcc cttggtgtca atattgacga attgctcttg tctcaaccag actcaggaga
     5761 gcaaggtctt gaaattgcgg gaaaattgat tgactcaggt gcagttgatc ttgtcgtagt
     5821 cgactcagtt gctgcccttg ttcctcgtgc ggaaattgat ggagatatcg gagatagcca
     5881 tgttggtttg caggctcgta tgatgagcca ggccatgcgt aaacttggcg cctctatcaa
     5941 taaaaccaaa acaattgcca tttttatcaa ccaattgcgt gaaaaagttg gagtgatgtt
     6001 tggaaatcca gaaacaacac cgggcggacg tgctttgaaa ttctatgctt cagtccgctt
     6061 ggatgttcgt ggtaatacac aaattaaggg aactggtgac caaaaagaaa ccaatgtcgg
     6121 taaagaaact aagattaagg ttgtaaaaaa taaggtagct ccaccgttta aggaagccgt
     6181 agttgaaatt atgtacggag aaggaatttc taagactggt gagcttttga agattgcaag
     6241 cgatttggat attatcaaaa aagcaggggc ttggtattct tacaaagatg aaaaaattgg
     6301 gcaaggttct gagaatgcta agaaatactt ggcagagcac ccagaaatct ttgatgaaat
     6361 tgataagcaa gtccgttcta aatttggctt gattgatgga gaagaagttt cagaacaaga
     6421 tactgaaaac aaaaaagatg agccaaagaa agaagaagca gtgaatgaag aagttacgct
     6481 tgacttaggc gatgaacttg aaatcgaaat tgaagaataa gctgttaaag cagtggagaa
     6541 atccgctact ttttcgattt ttgattcaag tttttagatt atatatagta gcttgaaata
     6601 agatatgaac aactttatta ggaaagtcaa attaatttct agaaatgttt tagcagctac
     6661 agcgtactat tccaaactca accaactata atagatcgaa actagaatag tacacatcta
     6721 cttctaaaac attgttaaaa atcgatttga ctgtccttat ttcattccgc tatattatat
     6781 atagttcgct gtttcttgtc gctcctctag gaagctgata taatagcttt atgaataaaa
     6841 aacgaacagt ggacctgata catggtccga ttcttccctc gctcttaagc ttcgccttcc
     6901 caatcttgct atcaaatatt tttcaacagc tctataacac tgctgatgtc ttgattgttg
     6961 gacgatttct tggtcaagaa tccttggctg caataggagc gacgacagcg atttttgacc
     7021 tgattgtagg ttttacactt ggtgttggca atggcatggg gattgtcatt gctcgttatt
     7081 atggggctcg gaatttcact aaaatcaagg aagcagtagc agccacctgg attttaggtg
     7141 ctcttttgag cattctagtt atgttgctgg gttttcttgg cttgtatcct ctcttgcaat
     7201 acttagatac tcctgcagaa attcttcctc aatcttatca atatatttct atgattgtga
     7261 cctgtgtagg tgtcagcttt gcttataatc tttttgcagg cttgttgcgg tctattggtg
     7321 acagtctagc agccctggga tttctgattt tctctgcctt ggttaatgtg gttctggatc
     7381 tctattttat tacgcaattg catctgggag ttcaatccgc aggacttgct accattattt
     7441 cacaaggttt atcagcggtt ctctgctttt attatattcg taaaagtgtg ccagaactct
     7501 tgccacagtt taaacatttc aaatgggaca aaagcttgta cgcggatctc ttggagcaag
     7561 gtttggctat gggcttgatg agttcaattg tatctatcgg cagtgtgatt ttacagtttt
     7621 ctgttaatac atttggtgca gtgattatta gtgcccagac ggcagctcga cgcattatga
     7681 cctttgccct tcttcctatg accgctattt ctgcatcaat gacgaccttt gcttctcaga
     7741 atctaggagc taagcgacct gaccgcattg ttcaaggtct tcgaatcggc agtcgtttaa
     7801 gtatatcctg ggcagttttt gtttgtattt tcctcttttt tgccagtcca gccttggttt
     7861 ccttcttggc tagttcgaca gatggttact tgatagaaaa tggaagtctc tatctgcaaa
     7921 tcagttcagc cttttatccc attttgagcc tcttgttgat ttatcgcaat tgcttgcagg
     7981 gcttggggca gaagatcctt cctctagttt ctagctttat tgaactaatc ggaaaaatcg
     8041 tttttgtggt tttgattatt ccttgggcag gatataaggg tgttatcctt tgtgaacctc
     8101 ttatctgggt tgccatgaca gttcaactgt acttctcact attccgtcat cccttgataa
     8161 aagaaggaaa ggcaattttg gcaaccaaag tgcaatccta gttggattta ctgaataaaa
     8221 tccatttcct ctagtgaaca tcgaaaaaac ttgtgttctc ttctttagtt tggtgttgaa
     8281 aatagtttaa cagacttttg acttctttta tatgatataa taaagtatag tatttatgaa
     8341 aaggacatat agagactgta aaaatatact tttgaaaagc tttttagtct ggggtgttat
     8401 tgtagataga atgcagacct tgtcagtcct atttacagtg tcaaaatagt gcgttttgag
     8461 gttctatcta caagcctaat cgtgactaag attgtcttct ttgtaaggta gaaataaagg
     8521 agtttctggt tctggattgt aaaaaatgag ttgttttaat tgataaggag tagaatatgg
     8581 aaattaatgt gagtaaatta agaacagatt tgcctcaagt cggcgtgcaa ccatataggc
     8641 aagtacacgc acactcaact gggaatccgc attcaaccgt acagaatgaa gcggattatc
     8701 actggcggaa agacccagaa ttaggttttt tctcgcacat tgttgggaac ggttgcatca
     8761 tgcaggtagg acctgttgat aatggtgcct gggacgttgg gggcggttgg aatgctgaga
     8821 cctatgcagc ggttgaactg attgaaagcc attcaactaa agaagagttc atgacggact
     8881 accgccttta tatcgaactc ttacgcaatc tagcagatga agcaggtttg ccgaaaacgc
     8941 ttgatacagg gagtttagct ggaattaaaa cgcacgagta ttgcacgaat aaccaaccaa
     9001 acaaccactc agaccatgtg gatccatacc cttacttggc aaaatggggc attagccgtg
     9061 agcagtttaa gtatgatatt gagaacggct tgacgattga aacaggctgg cagaagaatg
     9121 acactggcta ctggtacgta cattcagacg gctcttatcc aaaagacaag tttgagaaaa
     9181 tcaatggcac ttggtactac tttgacagtt caggctatat gcttgcagac cgctggagga
     9241 agcacacaga cggcaactgg tactggttcg acaactcagg cgaaatggct acaggctgga
     9301 agaaaatcgc tgataagtgg tactatttca acgaagaagg tgccatgaag acaggctggg
     9361 tcaagtacaa ggacacttgg tactacttag acgctaaaga aggcgccatg gtatcaaatg
     9421 cctttatcca gtcagcggac ggaacaggct ggtactacct caaaccagac ggaacactgg
     9481 cagacaagcc agaattcaca gtagagccag atggcttgat tacagtaaaa taataatgga
     9541 atgtctttca aatcagaaca gcgcatatta ttaggtcttg aaaaagctta atagtatgcg
     9601 tttttttgtg gagatatttc cttcaatgtt tgctactata ttaaacaaaa atcagaaagc
     9661 aaactagaaa gttatgctca aataaaatct aaatttgaca atgtaaaccg agtcggatag
     9721 ctttaagtac tgttttgagg ttgaagatac gatttttgat aggaactcat caattttaga
     9781 tttttaagca gcatcaataa attgcttcct tgttttgtca taattttttt atttaaaaaa
     9841 ttatgacaag agtgtgctat tctttttatg agaggtgtat gaatatgata aatgtatgtg
     9901 ataaatgtat gtgatgttgg aaaaagaata aaagaactta gaatatcttc aaatcttact
     9961 caagataaga ttgctgagta tttgtctttg aatcaaagca tgattgccaa aatggaaaaa
    10021 ggtgaaagga atatcacgaa tggatttaag taataaagct ccaaatctta gaaaaaagtt
    10081 gggagctgat ggtgaatcgc cgatagatat ttttaaattg gtacaaaaga tagaaaattt
    10141 gacgctggta ttttatggac tcggaaagaa tattagcgga gtctgttata aaggaactca
    10201 gttcagtctc attgcagtca attcagacat gtcattagga aggtaaagat tttctttagc
    10261 acatgaactg tatcatcttt attatgatga ggtgaagaag agttcagtca gtcttatctt
    10321 gattggtgaa ggagatgaaa ctgaaagaaa agcggatcag tttgcttctt attttttaat
    10381 tttcccatct tcactgtata ggatggttga ggaaattaga gaaaatgcca atagaactca
    10441 tcttgaagta gaagatatta taaaattggg tcagttttat ggtatcagtc ataaagctat
    10501 gttatataga ttgaggaatg atggatacct tgatgcagaa gaaattaaaa atatggatat
    10561 tagtgttata gagacagctt caagattagg ctatgataca agtttatatc gtcctttgtc
    10621 agaaagtaaa aaggaaacgg tgttaggata atatattaat tcaactgaac aacttttaga
    10681 aaataacaga atttcgcaag ggaagtatga ggaactgtta ctagatgctt tcagatatga
    10741 tattgtatat gggctatatg aagagggggg agttgtcgtt tgactagtcg tgtatttatt
    10801 gatgcagatt gtatttcagt atttttatgg gttggcactg aacatctttt agaaaagctc
    10861 tattcgggta aaattgttat tccacaagag gtgtatgatg aaatcaatat acctacaatt
    10921 ccccatttaa aatctaggat agaccagttg gtaactaagg gttcagctga gattgtgagc
    10981 atagacattg gaactgaaga atacgcatta tatagagatt taacaagaaa tcatgatagt
    11041 aacaagatta ttggtaaggg agaagtggct tctatttcct tagcgaaaaa gcataatggg
    11101 atattagaaa gtaataacct aagagatgtt aaaccatatg tagaagaatt ttctttagaa
    11161 catatgacaa caggagatat actgattgaa gcgtttaaag cgtaatttat tactgaacaa
    11221 gagggcaatc atatctggaa taatatgctt aaaaagagaa ggaaaattgg tgcaaattca
    11281 ttttcagact atcttcgtgg aagtattcat caaaatagac aaaaataaat ttggataaat
    11341 cgaactcact attcaggagg catatgagca attcgaaaaa gaaaagtgtc aaattgagcc
    11401 tataggatgt agaagtgaaa tagtaagtcc tgcatagtgg atgagagaaa agttctcctt
    11461 gaagttttcc tgaactatca gtcgcatgtc aaacgatatg tagggtaatg tgagagggga
    11521 tagcgagtag tttttggtta ttttatcaaa aaacttatat tttattatac cgaatgataa
    11581 aatataataa aaatgataga ataaggaaaa aacatgaatg tcaaaaagat aatgtcaatt
    11641 tttcaatcct tttatgttga tgtcagtatt gaggaactga ctttgacttt accaatcagt
    11701 tttgtaaaaa ggtttgagta tactcaaatg acttttcata aggaatcatt tttattgatt
    11761 aaagaaaaga gaagggggag tttgagttca tttgttactc aggctcgcac tatgggtgaa
    11821 aaagccaata tggatgttgt tttggtgttt tcgaagttat cagacagtga aaaaaagcaa
    11881 ttacttcaag ctagagttcc gtttgtagac tttaagggaa acctcttctt ccctccattg
    11941 ggactagtac tcaatgcgaa tgatactgaa gtccctaagg aattaacacc tagcgaacaa
    12001 ttaacgtgga ttgccttttt attgacaaaa ggtcaaaaag tagtagatgt tgatttgctt
    12061 tcacaagtca ctggacttcc aaactcaaca atttataggt gtttgaggac ttttaaagct
    12121 ttatattggt taaacaagca aaataagctt tacacatata cggtgtcaaa gaaagaatta
    12181 ttcttaaaat ccgtgtcatg tttatttaat cccatcaaaa aacggatttt attgccagat
    12241 ggcgatataa agcagataaa atctgtttct aaccttctat atggtggtgc ttatgctttg
    12301 tcgcattcaa cttttttagc tgaaacggat gaaaatatta gctatgtcat atggcagaga
    12361 aaattcaatc agttatcctt gccactttct cagcatgttt taaaatgaaa gatgctagag
    12421 atatggaaat atcgtccttt tgtatctgag ttttggaatg attttaaaaa taatcatgat
    12481 aaacaatttg tagatccgat ttctctttat ttgaccttaa aagatgatga tgacccacgt
    12541 atagaggaag agagtgaagc actagaaaat atgatattac agtatctggg agaagatgat
    12601 gccagctaat acgaaagtta tttttcaaga aatgtttgcg gattttcaga actattatgt
    12661 tctgattggg ggaactgcta cctctatcgt attggattcg caaggattta aaagtcgcac
    12721 aacaaaagat tatgatatgg tcatcattga tgaagtaaaa aataaggaat tttatactac
    12781 cttgaatcat tttttagaat tgggagagta tcaaggaagt cagaaagatg agaaagcgca
    12841 gctttttcga tttacaacaa ctaattctga gtttccttct atgattgaac tatttagtat
    12901 cttaccagaa tatccattaa agaaggacgg tcgagaaatt cccttatatt ttgaccaaga
    12961 tgctagttta tcagccttat tattggatga agattattat aatatattgg tgcatgtaaa
    13021 agaaaccatt caggggtatt cggtattgag taattgtggt ctatactctt cgaaaatctc
    13081 ttcaaaccac gtcagcttcc atctacaacc tcaaaacagt gttttgagca gcctgcagct
    13141 agcttcctag tttgctcttt gattttcatt gagtattaat tatttttaag gctaaagctt
    13201 ggctggatat gagggagcgc tctgccacag gtgctcaagg tttaagtaag tccattaaaa
    13261 agcatttgaa tgaccttacc cgtttgacag cttccttgct aggagatgaa aagttatcgg
    13321 ctataacatc aagtagtgcg gtaaaagcag acatgcaccg ctttgtgata gaattagagc
    13381 ctgtgaagtc aactattctt caaaataatg acatttcatt ggatcaaaat gaaatttttg
    13441 aaattctgaa aaattttctc gatggttaaa ataattgtag cgagatggct atattgaatt
    13501 cgtctatatc tggaaactag aaaaaacttc aatttcagga gaaaatgaag tcaatcttcc
    13561 cacaatcaaa cgtatagtat caaggttttt caagacctga tattatgcgt tttttgcttt
    13621 tcaaaacttt ttgcctagtc tttgttttta tcctctagtc acttgatttg tttcaggtgg
    13681 ttttttagta tagtgaaatg aaacgagaac aggacaaatt gatcaggaca gtcaaatcga
    13741 tttctaacaa tgttttagaa gcagagatgt actattctag tttcaatcta ctatagttaa
    13801 atctgcggtc aagtctactg gtgaatctat gattgtaata ctcttccaaa atctcatcaa
    13861 ccacgtcagt ctcgccttgc agtctgtatc ttactgacca agctagtgat ggacttagaa
    13921 taggtgattt ggagcgtcct attggctagg aaatgctgat catagtcctt tgctgaggct
    13981 agggtgtttc aacattcaac actcaactgg ttgatctagt tgataggaag ggaattacta
    14041 taaaatactt aggcttccat catatttttt gaaacgattg tgtaatcaaa atgtaccaat
    14101 attgtagtat tggtacagaa gatgttgtgt atggataaat atatcataac tgctgtctct
    14161 ctatgtaaaa agaaggctgt atcaaaaaga tttcatatgt ctgtgcatat atataataga
    14221 cttcctgcaa aactagaatc ctagttcatg attgataata ccagcactca aattcattcg
    14281 taatccgaag cgtttacgat gatttcgata ggttgttgaa aacattttaa acgtttttac
    14341 tttggcaaag atgttctcaa ccttgcttct ctccttggat agcgcatggt tataagcttt
    14401 atcttcagct attagcggct tgagtttgct ggatttacgt ggagtttgtg cttgaggata
    14461 tatcttcatg agcccttgat aaccactgtc agccaagatt ttaccagctt gtccgacttt
    14521 tttgataagc tgtttttaat ccagctaaca tctcttcaaa agtggtacgc tgaacaccaa
    14581 ca
//
LOCUS       DAVGJJ010000049        14344 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000049 DAVGJJ010000000
VERSION     DAVGJJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.049"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            213..1574
                     /gene="dnaA"
                     /locus_tag="VVE35_001338"
     CDS             213..1574
                     /gene="dnaA"
                     /locus_tag="VVE35_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU3061521.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            1733..2869
                     /gene="dnaN"
                     /locus_tag="VVE35_001339"
     CDS             1733..2869
                     /gene="dnaN"
                     /locus_tag="VVE35_001339"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU3061522.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            2934..3128
                     /locus_tag="VVE35_001340"
     CDS             2934..3128
                     /locus_tag="VVE35_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU3061523.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            3212..4327
                     /gene="ychF"
                     /locus_tag="VVE35_001341"
     CDS             3212..4327
                     /gene="ychF"
                     /locus_tag="VVE35_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU3061524.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            4398..4967
                     /locus_tag="VVE35_001342"
     CDS             4398..4967
                     /locus_tag="VVE35_001342"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU3061525.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTHVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            4968..8477
                     /gene="mfd"
                     /locus_tag="VVE35_001343"
     CDS             4968..8477
                     /gene="mfd"
                     /locus_tag="VVE35_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU3061526.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            8535..8801
                     /locus_tag="VVE35_001344"
     CDS             8535..8801
                     /locus_tag="VVE35_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU3061527.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            8794..9162
                     /locus_tag="VVE35_001345"
     CDS             8794..9162
                     /locus_tag="VVE35_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU3061528.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            9282..10550
                     /locus_tag="VVE35_001346"
     CDS             9282..10550
                     /locus_tag="VVE35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU3061529.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGELTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            10547..11824
                     /gene="tilS"
                     /locus_tag="VVE35_001347"
     CDS             10547..11824
                     /gene="tilS"
                     /locus_tag="VVE35_001347"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU3061530.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            11828..12370
                     /gene="hpt"
                     /locus_tag="VVE35_001348"
     CDS             11828..12370
                     /gene="hpt"
                     /locus_tag="VVE35_001348"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU3061531.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYVGVLKEEVYSN"
     gene            12386..14344
                     /gene="ftsH"
                     /locus_tag="VVE35_001349"
     CDS             12386..14344
                     /gene="ftsH"
                     /locus_tag="VVE35_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU3061532.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         4933 a         2196 c         2863 g         4352 t
ORIGIN      
        1 ggctgttctt ttattttttt atctcacaag gttatccact atgtttttcg ataaaaagct
       61 taataaatca ataatttctt cttttatccc caacctgtgg ataaagtttg gtaacattgt
      121 ggattatttt tcacagcttg tggaaaattc ttgctatcta tggtaaaata tctctagtat
      181 taaactttta aatagtaaag gaggagaaag gattgaaaga aaaacaattt tggaatcgta
      241 tattagaatt tgcacaagaa agactgactc gatccatgta tgatttctat gctattcaag
      301 ctgaactcat caaggtagag gaaaatgttg ccactatatt tctacctcgc tctgaaatgg
      361 aaatggtctg ggaaaaacaa ctaaaagata ttattgtagt agctggtttt gaaatttatg
      421 acgctgaaat aactccccac tatattttca ccaaacctca agatacgact agctcacaag
      481 ttgaagaagc tacaaattta actctttatg actatagtcc aaagttagta tctattcctt
      541 attcagatac gggattaaaa gaaaagtata cctttgataa ctttattcaa ggggatggaa
      601 atgtttgggc tgtatcagcc gctttagctg tctctgaaga tttggctctg acctataacc
      661 ctctttttat ctatggagga ccaggccttg gtaagactca cttattaaac gctattggaa
      721 atgaaattct aaaaaatatt cctaatgcgc gtgttaaata tatccctgcc gaaagcttta
      781 ttaatgactt tcttgatcac ctaagacttg gggaaatgga aaagtttaaa aagacctatc
      841 gtagtcttga tcttttgtta atcgatgata tccagtcact cagcggaaaa aaagtcgcaa
      901 ctcaggaaga atttttcaat acctttaacg cccttcatga caagcaaaaa caaattgtcc
      961 taacgagtga tcgtagtcca aaacatctag aagggctcga ggagaggctt gtcacgcgtt
     1021 ttagttgggg attgacacaa actatcacac cccctgactt tgaaacacgt attgccattt
     1081 tacaaagtaa aacggaacat ttaggctaca atttccaaag tgatactcta gaatacctag
     1141 ctgggcaatt tgattcaaat gttcgagatc ttgagggagc catcaacgac atcactttaa
     1201 ttgccagagt aaaaaaaatc aaggatatca ctattgatat tgctgcagaa gccattagag
     1261 cccgcaaaca agatgttagc caaatgctcg tcatcccaat tgataaaatc caaactgaag
     1321 ttggtaactt ttatggtgtt agtatcaaag aaatgaaggg aagtagacgc cttcaaaata
     1381 ttgttttggc ccgtcaagta gccatgtatt tatctagaga actaacagat aatagtcttc
     1441 caaaaattgg gaaggaattt gggggaaaag atcataccac agtcattcat gcccatgcca
     1501 aaataaaatc tttgattgat caagacgata atttacgttt agaaattgaa tcaatcaaaa
     1561 agaaaatcaa ataatttgtg gataactttt agttttttat cttttttatc cacatttttt
     1621 aaacaagcta aaaaacttga tatgacttgt ttaaaggctg ttttccacag atttcacaga
     1681 ctctattatt actattatct ttctaatact aaaaataaat aaaggagaat ccatgattca
     1741 tttttcaatt aataaaaatt tatttctaca agcattaaat actactaaga gagctattag
     1801 ttctaaaaat gccattccta ttttatcaac agtaaaaatt gacgtgacca atgaaggtat
     1861 tactttaatt ggttcaaatg gtcaaatttc aattgaaaat tttatttctc aaaaaaatga
     1921 agatgctggt ttgttaatta cttctttagg ttcgatcctt cttgaagctt ctttctttat
     1981 caatgtagta tctagtttac ctgatgtaac tcttgatttt aaagaaattg aacaaaatca
     2041 aattgtttta accagtggca aatcagaaat taccctaaaa ggaaaagata gcgaacaata
     2101 tccacgaatc caagaaattt cagcaagcac tcctttaata cttgaaacaa aattactcaa
     2161 gaaaattatt aatgaaacag cctttgctgc aagtacacaa gagagtcgtc cgattttaac
     2221 aggtgtccac ttcgtattga gtcaacacaa agagttaaaa acagttgcaa cagactctca
     2281 tcgcctaagc cagaaaaaat tgactcttga aaaaaatagt gatgattttg atgtcgtaat
     2341 tcctagccgt tctctacgcg aattttcagc ggtatttaca gatgatatcg aaactgtaga
     2401 gattttcttt gccaataacc aaatcctctt tagaagcgaa aatattagct tctatactcg
     2461 tctcctagaa ggaaactatc ctgatacaga tcgcttgatt ccaacagact ttaacactac
     2521 tattactttt aatgtggtaa acttacgcca gtcaatggag cgtgcccgtc ttttatcaag
     2581 tgcgactcaa aatggtactg tgaaacttga aattaaggat ggggttgtta gcgcccatgt
     2641 tcactctcca gaagttggta aagtaaacga agaaatcgat actgatcagg ttactggtga
     2701 agatttgacc attagtttca acccaactta cttgattgat tctcttaaag ctttaaatag
     2761 cgaaaaggtg accattagct ttatctcagc tgttcgtcca tttactcttg tgccagcaga
     2821 tactgacgaa gacttcatgc agctcattac accagttcgt acaaattaag tgaaagaggt
     2881 tgagcctggc ttgcctcttt tatgatataa tcgaaaaaga aaaggagagt agtatgtatc
     2941 aagttggaaa ttttgttgag atgaaaaaat cacacgcttg tacaatcaag tcgactggta
     3001 aaaaggctaa tcgttgggaa attacacgtg taggagcaga tatcaaaata aaatgcagta
     3061 attgtgagca tgttgtcatg atggggcgat atgattttga gcgaaaaatg aataaaatta
     3121 ttgactgaga acccttagtt agagggttag cactttatcc ctttttgtgt tataatatta
     3181 gggattgaaa tgaaaacgga gaatgagaaa tatggctttg acagcaggta tcgttggttt
     3241 gccaaacgtt ggtaaatcaa cactatttaa tgcaattaca aaagcaggag cagaggcagc
     3301 aaactaccca tttgcgacta ttgatccaaa tgttggaatg gtggaagttc cagatgaacg
     3361 cctacaaaaa ctaactgaaa tgataactcc taaaaagaca gttcccacaa catttgaatt
     3421 tacggatatt gcagggattg taaaaggagc ttcaaaagga gaagggctag ggaataaatt
     3481 cttggccaat attcgtgaag tagatgcgat tgttcacgta gttcgtgctt ttgatgatga
     3541 aaatgtgatg cgcgagcaag gacgtgaaga cgcctttgta gatccacttg cagatattga
     3601 tacaattaat ctggaattaa ttcttgctga cttagaatca gtgaacaaac gatatgcgcg
     3661 tgtagaaaag atggcacgta cgcaaaaaga taaagaatca gtagcagaat tcaatgttct
     3721 tcaaaagatt aaaccagtcc tagaagacgg gaaatcagct cgtaccattg aatttacaga
     3781 tgaggaacaa aaggttgtca aaggtctttt ccttttgacg actaaaccag ttctttatgt
     3841 agctaatgtg gacgaggatg tggtttcaga acctgactct atcgactatg tcaaacaaat
     3901 tcgtgaattt gcagcgacag aaaatgctga agtagtcgtt atttctgcgc gtgctgagga
     3961 agaaatttct gaattggatg atgaagataa aaaagagttt cttgaagcca ttggtttgac
     4021 agaatcaggt gtagataagt tgacgcgtgc agcttaccac ttgcttggat tgggaactta
     4081 cttcacagct ggtgaaaaag aagttcgcgc ttggactttc aaacgtggta tgaaggctcc
     4141 tcaagcagct ggtattatcc actcagactt tgaaaaaggc tttattcgtg cagtaaccat
     4201 gtcatatgaa gatctagtga aatacggatc tgaaaaggcc gtaaaagaag ctggacgctt
     4261 gcgtgaagaa ggaaaagaat atatcgttca agatggcgat atcatggaat tccgctttaa
     4321 tgtctaaaaa ttaataaatg gtgtcaatta ggttggaaaa aaattccaac ccttttggct
     4381 tttgaaagga aaaataaatg accaaattac ttgtaggctt gggaaatcca ggggataaat
     4441 attttgaaac aaaacacaat gttggtttta tgttgattga tcaactagcg aagaaacaga
     4501 atgtcacttt tacacacgat aagatatttc aagctgacct agcatccttt ttcctaaatg
     4561 gagaaaaaat ttatctggtt aaaccaacga cctttatgaa tgaaagtgga aaagcagttc
     4621 atgctttatt aacttactat ggtttggata ttgacgattt acttatcatt tacgatgatc
     4681 ttgacatgga agttgggaaa attcgtttaa gagcaaaagg ctcagcaggt ggtcataatg
     4741 gtatcaagtc tattattcaa catataggaa ctcatgtctt taaccgtgtt aagattggaa
     4801 ttggaagacc taaaaatggt atgtcagttg ttcatcatgt tttgagtaag tttgacaggg
     4861 atgattatat cggtatttta cagtctgttg acaaagttga cgattctgta aactactatt
     4921 tacaagagaa aaattttgag aaaacaatgc agaggtataa cggataaatg gtgaccttat
     4981 tagatttatt ctcagaaaat gatcagatta aaaaatggca tcaaaattta acagataaga
     5041 aaagacaact aatacttggt ttatcaacat ctactaaggc tcttgcaatt gcaagcagtt
     5101 tagaaaaaga agataggatt gtgttattga cgtcaactta tggagaagca gaaggacttg
     5161 ttagtgatct tatatctatc ttgggtgagg aactcgtcta tccatttttg gtagatgatg
     5221 ctcctatggt ggagtttttg atgtcttcac aggaaaaaat tatttcacgg gttgaagcct
     5281 tgcgtttttt gactgattca tctaagaaag ggattttagt ttgtaatatc gcagcaagtc
     5341 gattgatttt accgtctccc aatgcattca aagatagtat tgtaaaaatc tcagttggtg
     5401 aagaatatga tcaacacgcg tttatccatc agttaaagga aaatggctat cgaaaagtta
     5461 ctcaagtaca aactcagggc gaatttagtc ttcgaggaga tattttagat atttttgaaa
     5521 tatcccagtt agaaccttgt cgaattgagt tttttggtga tgaaattgat ggtatcaggt
     5581 catttgaagt agaaacacaa ttatcgaaag aaaataagac agaactcact atctttccag
     5641 ctagtgatat gcttttgaga gaaaaggatt atcaacgagg acagtcagct ttagaaaaac
     5701 aaatttcaaa aactttatca cctattttga aatcatacct agaagaaatt ctttcaagtt
     5761 ttcaccaaaa acaaagtcat gcagactctc ggaagttttt atctttgtgc tatgataaga
     5821 catggactgt ctttgattat attgaaaaag atactccaat attctttgat gattatcaaa
     5881 aattgatgaa tcagtatgaa gtctttgaaa gagaattagc gcagtacttt acagaagaat
     5941 tacagaatag taaagcattt tctgatatgc agtatttttc tgatattgaa caaatctata
     6001 aaaaacaaag tccagtgacc tttttttcta atcttcaaaa gggtttagga aatctcaaat
     6061 ttgacaaaat ttatcaattc aatcaatatc ctatgcagga atttttcaat cagttttctt
     6121 ttctaaaaga agaaattgaa cgatataaaa aaatggatta caccattatt ctgcagtcta
     6181 gcaattcaat gggaagtaaa acattggagg atatgttaga ggaatatcag attaaattgg
     6241 attctagaga taagacaaat atctgtaaag aatctgtaaa cttaatagag ggtaatctca
     6301 gacatggttt tcattttgta gatgaaaaga ttttattgat aactgaacat gagatttttc
     6361 aaaagaaatt aaagcgtcgt tttcgaagac aacatgtttc aaatgcagag agattaaaag
     6421 attacaatga acttgaaaaa ggggactatg ttgtccatca tatccatggg attggtcaat
     6481 atctaggaat tgaaaccatt gaaatcaagg gaattcatcg tgattatgtc agtgtccaat
     6541 accaaaatgg tgatcaaatt tctattcccg tggaacagat tcatctactg tccaaatata
     6601 tttcaagtga tggtaaagct ccaaaactca ataaattaaa tgacggtcat tttaaaaagg
     6661 ccaagcaaaa ggttaagaac caggtagagg atatagctga tgatttaatc aaactctact
     6721 ctgaacgtag tcagttgaag ggttttgctt tctcagctga tgatgatgat caagatgcct
     6781 ttgatgatgc tttcccttat gttgaaacgg atgatcaact tcgtagtatt gaggaaatca
     6841 agagggatat gcaggcttct cagccaatgg atcgactttt agttggggat gttggttttg
     6901 gaaagactga agttgctatg cgtgcagcct ttaaagcagt caatgatcac aaacaggttg
     6961 tcattctagt tccgacgacg gttttagcgc aacagcacta tacgaatttt aaggaacgat
     7021 tccaaaattt tgcagttaat attgatgtgt tgagtcgctt tagaagtaaa aaagagcaga
     7081 ctgcaacact tgaaaaattg aaaaacggtc aagtcgatat tttgattgga acacatcgtg
     7141 ttttgtcaaa agatgttgtg tttgctgatt tgggcttgat gattattgat gaggaacagc
     7201 gatttggtgt caagcataag gaaactttga aagaactgaa gaaacaagtg gatgtcctaa
     7261 ccttgaccgc tacgccaatc cctcgtaccc tccatatgtc tatgctggga atcagagatt
     7321 tatctgttat tgaaactccg ccgactaatc gctatcctgt tcagacctat gttttggaaa
     7381 agaatgatag tgtcattcgt gatgctgtct tgcgtgaaat ggagcgtgga ggtcaagttt
     7441 attatcttta caacaaagtt gacacaattg ttcagaaggt ttcagaatta caggagttga
     7501 ttccggaggc ttcgattgga tatgttcatg gtcgaatgag tgaagtccag ttggaaaata
     7561 ctctattaga ctttattgag ggacaatacg atatcttggt gacgactact attattgaga
     7621 caggggtgga cattccaaat gctaatactt tatttattga aaatgcggac catatgggct
     7681 tgtcaacctt atatcagtta agaggaagag tcggtcgtag taatcgtatt gcttatgctt
     7741 atctcatgta tcgtccagaa aaatcaatca gtgaagtctc tgaaaagaga ttagaagcga
     7801 ttaaaggatt tacagaattg ggctctggct ttaagattgc aatgcgagat ctttcgattc
     7861 gtggagcagg aaatctttta ggaaaatccc agtctggttt cattgattct gttggttttg
     7921 aattgtattc gcagttatta gaggaagcta ttgctaaacg aaacggtaat gctaacgcta
     7981 acacaagaac caaagggaat gctgagttga ttttgcaaat tgatgcctat cttcctgata
     8041 cttatatttc tgatcaacga cataagattg aaatttacaa gaaaattcgt caaattgaca
     8101 accgtgtcaa ttatgaagag ttacaagagg agttgataga ccgttttgga gaatacccag
     8161 atgtagtagc ctatctttta gagattggtt tggtcaaatc atacttggac aaggtctttg
     8221 ttcaacgtgt ggaaagaaaa gataataaaa ttacaattca atttgaaaaa gtcactcaac
     8281 gactgttttt agctcaagat tattttaaag ctttatccgt aacgaactta aaagcaggca
     8341 tcgctgagaa taaggaatta atggagcttg tatttgatgt tcaaaataag aaagattatg
     8401 aaattttaga aggtctgctg atttttggag aaagtttatt agagataaaa gagtctaagg
     8461 aagaaaattc catttgatat ttttcttcta taaaatagat aaaaatggta caataataaa
     8521 ttgaggtaat aaggatgaga ttagataaat atttaaaagt atcgcgaatt atcaagcgtc
     8581 gtacagtcgc aaaggaagta gcagataaag gtagaatcaa ggttaatgga atcttggcca
     8641 aaagttcaac ggacttgaaa gttaatgacc aagttgaaat tcgctttggc aataagttgc
     8701 tgcttgtaaa agtactagag atgaaagata gtacaaaaaa agaagatgca gcaggaatgt
     8761 atgaaattat cagtgaaaca cgggtagaag aaaatgtcta aaaatattgt acaattgaat
     8821 aattctttta ttcaaaatga ataccaacgt cgtcgctacc tgatgaaaga acgacaaaaa
     8881 cggaatcgtt ttatgggagg ggtattgatt ttgattatgc tattatttat cttgccaact
     8941 tttaatttag cgcagagtta tcagcaatta ctccaaagac gtcagcaatt agcagacttg
     9001 caaactcagt atcaaacttt gagtgatgaa aaggataagg agacagcatt tgctaccaag
     9061 ttgaaagatg aagattatgc tgctaaatat acacgagcga agtactatta ttctaagtcg
     9121 agggaaaaag tttatacgat tcctgacttg cttcaaaggt gataaaatgg aaaatttatt
     9181 agacgtaata gagcaatttt tgggtttgtc agatgaaaag ttggaagaat tggctgataa
     9241 aaatcaatta ttgcgtttac aagaagaaaa ggaaaggaag aatgcgtaaa ttcttaatta
     9301 ttttgttgct accaagtttt ttgaccattt caaaagtcgt tagcacagaa aaagaagtcg
     9361 tctatacttc gaaagaaatt tattaccttt cacaatctga ctttggtatt tattttagag
     9421 aaaaattaag ttctcccatg gtttatggag aggttcctgt ttatgcgaat gaagatttag
     9481 tagtggaatc tggggaattg actcccaaaa caagttttca aataaccgag tggcgcttaa
     9541 ataaacaagg gattccagta tttaagctat caaatcatca atttatagct gcggacaaac
     9601 gatttttata tgatcaatca gaggtaactc caacaataaa aaaagtatgg ttagaatctg
     9661 actttaaact gtacaatagt ccttatgatt taaaagaagt gaaatcatcc ttatcagctt
     9721 attcgcaagt atcaatcgac aagaccatgt ttgtagaagg aagagaattt ctacatattg
     9781 atcaggctgg atgggtagct aaagaatcaa cttctgaaga agataatcgg atgagtaaag
     9841 ttcaagaaat gttatctgaa aaatatcaga aagattcttt ctctatttat gttaagcaac
     9901 tgactactgg aaaagaagct ggtatcaatc aagatgaaaa gatgtatgca gccagcgttt
     9961 tgaaactctc ctatctctat tatacgcaag aaaaaataaa tgagggtctt tatcagttag
    10021 atacgactgt aaaatacgta tctgcagtca atgattttcc aggttcttat aaaccagagg
    10081 gaagtggtag tcttcctaaa aaagaagata ataaagaata ttctttaaag gatttaatta
    10141 cgaaagtatc aaaagaatct gataatgtag ctcataatct attgggatat tacatttcaa
    10201 accaatctga tgccacattc aaatccaaaa tgtctgccat tatgggagat gattgggatc
    10261 caaaagaaaa attgatttct tctaagatgg ccgggaagtt tatggaagct atttataatc
    10321 aaaatggatt tgtgctagag tctttgacta aaacagattt tgatagtcag cgaattgcca
    10381 aaggtgtttc tgttaaagta gctcataaaa ttggagatgc ggatgaattt aagcatgata
    10441 cgggtgttgt ctatgcagat tctccattta ttctttctat tttcactaag aattctgatt
    10501 atgatacgat ttctaagata gccaaggatg tttatgaggt tctaaaatga gggaaccaga
    10561 ttttttaaat cattttctca agaagggata tttcaaaaag catgctaagg cggttctagc
    10621 tctttctggt ggattagatt ctatgtttct atttaaggta ttgtctactt atcaaaaaga
    10681 gttagagatt gaattgattc tggctcatgt gaatcataag cagagaattg aatcagattg
    10741 ggaagaaaag gaattaagga agttggctgc tgaagcagag cttcctattt atatcagcaa
    10801 tttttcagga gaattttcag aagcgcgtgc acgaaatttt cgttatgatt tttttcaaga
    10861 ggttatgaaa aagacaggtg cgacagcttt agtcactgcc caccatgctg atgatcaggt
    10921 ggaaacgatt ttgatgcgct tgattcgagg aactcgcttg cgctatctat caggaattaa
    10981 ggagaagcaa gtagtcggag agatagaaat cattcgtccc ttcttgcatt ttcagaaaaa
    11041 agactttcca tcaatttttc actttgaaga tacatcaaat caggagaatc attattttcg
    11101 aaatcgtatt cgaaactctt acttaccaga attggaaaaa gaaaatcctc gatttaggga
    11161 tgcaatctta ggcatcggca atgaaatttt agattatgat ttggcaatag ctgaattatc
    11221 taacaatatt aatgtggaag atttacagca gttattttct tactctgagt ctacacaaag
    11281 agttttactt caaacttatc tgaatcgttt tccagatttg aatcttacaa aagctcagtt
    11341 tgctgaagtt cagcagattt taaaatctaa aagccagtat cgtcatccga ttaaaaatgg
    11401 ctatgaattg ataaaagagt accaacagtt tcagatttgt aaaatcagtc cgcaggctga
    11461 tgaaaaggaa gatgaacttg tgttacacta tcaaaatcag gtagcttatc aaggatattt
    11521 attttccttt ggacttccat tagaaggtga atcaattcaa caaatacctg tttcacgtga
    11581 aacatccata cacattcgtc atcgaaaaac aggagatgtt ttgattcaaa atgggcatag
    11641 aaaaaaactc agacgtttat ttattgattt gaaaatccct atggaaaaga gaaactctgc
    11701 tcttattatt gagcaatttg gtgaaattgt ctcaattttg ggaattgcga ccaataattt
    11761 gagtaaaaaa acgaaaaatg atataatgaa cactgtactt tatatagaaa aaatagatag
    11821 gtaaaaaatg ttagaaaacg atattaaaaa agtcctcgtt tcacacgatg aaattacaga
    11881 agcagctaaa aaactaggtg ctcaattaac taaagattat gcaggaaaaa atccaatctt
    11941 agttgggatt ttaaaaggat ctattccttt tatggctgaa ttggtcaaac atattgatac
    12001 acatattgaa atggacttca tgatggtttc tagctaccat ggtggaacag caagtagtgg
    12061 tgttatcaat attaaacaag atgtgactca agatatcaaa ggaagacatg ttctatttgt
    12121 agaagatatc attgatacag gtcaaacttt gaagaatttg cgagatatgt ttaaagcaag
    12181 agaagcagct tctgttaaaa ttgcaacctt gttggataaa ccagaaggac gtgttgtaga
    12241 aattgaggca gactatacct gctttactat cccaaatgag tttgtagtag gttatggttt
    12301 agactacaaa gaaaattatc gtaatcttcc ttatgttgga gtattgaaag aggaagtgta
    12361 ttcaaattag aaagaataat ctttaatgaa aaaacaaaat aatggtttaa ttaaaaatcc
    12421 ttttctatgg ttattattta tctttttcct tgtgacagga ttccagtatt tctattctgg
    12481 gaataactca ggaggaagtc agcaaatcaa ctatactgag ttggtacaag aaattaccga
    12541 tggtaatgta aaagaattaa cttaccaacc aaatggtagt gttatcgaag tttctggtgt
    12601 ctataaaaat cctaaaacaa gtaaagaagg aacaggtatt cagtttttca cgccatctgt
    12661 tactaaggta gagaaattta ccagcactat tcttcctgca gatactaccg tatcagaatt
    12721 gcaaaaactt gctactgacc ataaagcaga agtaactgtt aagcatgaaa gttcaagtgg
    12781 tatatggatt aatctactcg tatccattgt gccatttgga attctattct tcttcctatt
    12841 ctctatgatg ggaaatatgg gaggaggcaa tggccgtaat ccaatgagtt ttggacgtag
    12901 taaggctaaa gcagcaaata aagaagatat taaagtaaga ttttcagatg ttgctggagc
    12961 tgaggaagaa aaacaagaac tagttgaagt tgttgagttc ttaaaagatc caaaacgatt
    13021 cacaaaactt ggagcccgta ttccagcagg tgttcttttg gagggacctc cggggacagg
    13081 taagactttg cttgctaagg cagtcgctgg agaagcaggt gttccattct ttagtatctc
    13141 aggttctgac tttgtagaaa tgtttgtcgg agttggagct agtcgtgttc gctctctttt
    13201 tgaggatgcc aaaaaagcag caccagctat catctttatc gatgaaattg atgctgttgg
    13261 acgtcaacgt ggagtcggtc tcggcggagg taatgacgaa cgtgaacaaa ccttgaacca
    13321 acttttgatt gagatggatg gttttgaggg aaatgaaggg attatcgtca tcgctgcgac
    13381 aaaccgttca gatgtacttg atcctgccct tttgcgtcca ggacgttttg atagaaaagt
    13441 attggttggc cgtcctgatg ttaaaggtcg tgaagcaatc ttgaaagttc acgctaagaa
    13501 caagccttta gcagaagatg ttgatttgaa attagtggct caacaaactc caggctttgt
    13561 tggtgctgat ttagagaatg tcttgaatga agcagcttta gttgctgctc gtcgcaataa
    13621 atcgataatt gatgcttcag atattgatga agcagaagat agagttattg ctggaccttc
    13681 taagaaagat aagacagttt cacaaaaaga acgagaattg gttgcttacc atgaggcagg
    13741 acataccatt gttggtctag tcttgtcgaa tgctcgcgtt gtccataagg ttacaattgt
    13801 accacgcggc cgtgcaggcg gatacatgat tgcacttcct aaagaggatc aaatgcttct
    13861 atctaaagaa gatatgaaag agcaattggc tggcttaatg ggtggacgtg tagctgaaga
    13921 aattatcttt aatgtccaaa ctacaggagc ttcaaacgac tttgaacaag cgacacaaat
    13981 ggcacgtgca atggttacag agtacggtat gagtgaaaaa cttggcccag tacaatatga
    14041 aggaaaccat gctatgcttg gtgcacagag tcctcaaaaa tcaatttcag aacaaacagc
    14101 ttatgaaatt gatgaagagg ttcgttcatt attaaatgag gcacgaaata aagctgctga
    14161 aattattcag tcaaatcgtg aaactcacaa gttaattgca gaagcattat tgaaatacga
    14221 aacattggat agtacacaaa ttaaagctct ttacgaaaca ggaaagatgc ctgaagcagt
    14281 agaagaggaa tctcatgcac tatcctatga tgaagtaaag tcaaaaatga atgacgaaaa
    14341 ataa
//
LOCUS       DAVGJJ010000050        13510 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000050 DAVGJJ010000000
VERSION     DAVGJJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.050"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            87..1265
                     /locus_tag="VVE35_001350"
     CDS             87..1265
                     /locus_tag="VVE35_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU3061533.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NELIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(1493..1939)
                     /locus_tag="VVE35_001351"
     CDS             complement(1493..1939)
                     /locus_tag="VVE35_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061534.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
     gene            2009..2668
                     /locus_tag="VVE35_001352"
     CDS             2009..2668
                     /locus_tag="VVE35_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU3061535.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(2720..3871)
                     /locus_tag="VVE35_001353"
     CDS             complement(2720..3871)
                     /locus_tag="VVE35_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU3061536.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3886..4617)
                     /locus_tag="VVE35_001354"
     CDS             complement(3886..4617)
                     /locus_tag="VVE35_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061537.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADLENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            4846..4947
                     /locus_tag="VVE35_001355"
                     /pseudo
     CDS             4846..4947
                     /locus_tag="VVE35_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(4919..5695)
                     /locus_tag="VVE35_001356"
     CDS             complement(4919..5695)
                     /locus_tag="VVE35_001356"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU3061538.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQV
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(5688..6911)
                     /gene="trpB"
                     /locus_tag="VVE35_001357"
     CDS             complement(5688..6911)
                     /gene="trpB"
                     /locus_tag="VVE35_001357"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU3061539.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(6889..7488)
                     /locus_tag="VVE35_001358"
     CDS             complement(6889..7488)
                     /locus_tag="VVE35_001358"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU3061540.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFSPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSQVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(7475..8242)
                     /gene="trpC"
                     /locus_tag="VVE35_001359"
     CDS             complement(7475..8242)
                     /gene="trpC"
                     /locus_tag="VVE35_001359"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU3061541.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(8239..9243)
                     /gene="trpD"
                     /locus_tag="VVE35_001360"
     CDS             complement(8239..9243)
                     /gene="trpD"
                     /locus_tag="VVE35_001360"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU3061542.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(9254..9820)
                     /locus_tag="VVE35_001361"
     CDS             complement(9254..9820)
                     /locus_tag="VVE35_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU3061543.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(9817..11178)
                     /gene="trpE"
                     /locus_tag="VVE35_001362"
     CDS             complement(9817..11178)
                     /gene="trpE"
                     /locus_tag="VVE35_001362"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU3061544.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIENALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYLYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(11818..11898)
                     /locus_tag="VVE35_001363"
                     /pseudo
     CDS             complement(11818..11898)
                     /locus_tag="VVE35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(12500..13501)
                     /locus_tag="VVE35_001364"
     CDS             complement(12500..13501)
                     /locus_tag="VVE35_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU3061545.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         3896 a         3032 c         2330 g         4252 t
ORIGIN      
        1 aaagcttaaa aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga
       61 atctgacaac taggaagaaa actaatatga ccttatctaa gaaacaatta caactacgag
      121 ctaaaatact ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta
      181 aaacaggaat aacacctgca acaactagca gtatcacaaa tgagctgatt aaagaaaaca
      241 ttctcttaga acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc
      301 tagacattca agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca
      361 cttttgcttt aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta
      421 aacagttaat ccaagaaaag ggaaatcaac tcattagcca gacactaaag caattcttaa
      481 ataactgctc tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag
      541 atgattataa aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc
      601 aatcccactt tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta
      661 aacgcctatt tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag
      721 gtatgcactg ttcttatatc tacgacggaa acatttatgg aaaaggaaat ctaatgattg
      781 gagaaatagg gcatacagtt gtttcatctg agggagaaga atgtagttgc gggcgcaagg
      841 gatgcttaca aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc
      901 atcaatctcc ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc
      961 aagttatttt aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag
     1021 ccttactcta tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa
     1081 tctacctaca tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa
     1141 tgaactacaa acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca
     1201 atgattttac agccgcacac tcagctattg cactttgttt gtatcataca attctacatt
     1261 cctaattttc tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg
     1321 aacaaacctg ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat
     1381 tatactccta tctccccttt ttcataatgt atagtaaaat gaaataataa caggacgaat
     1441 cgatcgggac agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta
     1501 gtttcaatat actatatttt cgtacaggtg cttcaaccat ttgaacgatt tcaaatcctt
     1561 ctttttggta aagattctga gctctttgat ttgcctcgaa gacatttaga gaaatactat
     1621 ctatatcttc attttcaaag gccaaactaa caaatttcct taaagcctgg ctacctaagc
     1681 cttgctcctg tttctggggg ttgataaaaa atctcccgat atgaagattg ctgtcttcta
     1741 gcctgatttt ctggataaat cccacaaact cttgttcatc aaagattgaa aagactcctt
     1801 ccaaggcttg aagggtcagt agaaaaggaa tccttggtcc catccattgt tcttgaaagg
     1861 atttgcctag ggagttggac cactggcata caaattgagc gttttctgtg ctcacctttt
     1921 cttcaaaacg aattgtcatc ttttcctcac caccttatct atgtttctcc attatactat
     1981 ttctcccatt ttttacgaat agataagtat gattgatttt tatttttttc tcgtcgggag
     2041 cattctcgct tcctttcttg gtttggtcat tgaccgtttt ccagagcaat ccattatcag
     2101 ttcagccagt cactgcgatt cctgtcagac tcgcttgcgt cccttagatt tgattccgat
     2161 tctctcacag gtcttcaatc gctttcgctg tcgctactgc aaagttcgct atcctgtctg
     2221 gtatgccctc tttgaattag tcttaggact cctctttctg ctttactctt gggaattgct
     2281 ttccttgggg caagtcgtcc taatcaccgc tggtttgacc ttgggtatct acgactttca
     2341 ccatcaggaa tatcccttac tggtctggat gactttccac ctaatcctaa tagcttcctc
     2401 tggctggaat ctggtcatgg tctccttcct tgctcttgga attttggctc attttatcga
     2461 tatccgcatg ggcgcaggag atttcctctt tttagcttct tgtgctctcg tctttagcgt
     2521 aacggagtta ctgatcttga ttcagtttgc ttctgcgacg ggtatcctgg cctttatcct
     2581 gcaaaagaaa aaggaaagac ttcctttcgt gcctttcctc ttacttgcta cttgtttgat
     2641 tatttttggt aagctactgc ttgtctgata aaatccaatt tctgccatat gtccttcatg
     2701 aaattatttc acagttaaat tataaattat ttcttttgta caaagggatg atgttatcaa
     2761 atcgatctgt tcttctatct tcttgatact gatcaaaaaa tttcatttcg actgaaaata
     2821 tttcacttat aaactgtaaa cgaatacttt gtttagacat tatagtcgct agactgacta
     2881 gatgattact caaaacgacg tccagaatac tctttacttt gcttggtttt ttaacaaaaa
     2941 tttgatcatc caaaggttca atcattttgt aacctttttg cgcaatttga cgataaaagt
     3001 aagaatgttg ctttggagtc aataatccta acttaaaagc tcgatactct aaagcctgta
     3061 tcgaaacatt caaatccgac ttcaataaaa tataactatc aggattgctg acacgcttgc
     3121 caaccctctc ttcaaatttg actaaaaact cttcttttgg caataaaaaa catgatgcaa
     3181 aataatttgc ttcttgctcc aaacgatcgc catcttcatt catatcttta tatttatgta
     3241 aaagaatatg tcctagctca tgagctaagt caaaatttcg acgtacagat gatttattcg
     3301 ttcccaacac aatataaggt cttcccaatt ttgaccatgc gctataagca tcagcttggc
     3361 cattaattaa tcgttccacg atatagatgc ctgaacgttc taatttataa agcaaatcat
     3421 gattatcttt tgaaatacct aatttttccc tggcataaag agccaattcc tcaatggatt
     3481 ctcccttatg ataagattca ctcactacat tacttaggtc atgaattata atattaggta
     3541 taattacaaa actttcaaaa taatcaatca aactatctac cttatgtaaa tacatagttt
     3601 gaatatctat tgttttccgt gttgctaggt ctgcatttct aaaggcaatt acagaagaat
     3661 caaatcgaat gctctcttct tcctgttcaa aataagttaa atcaacatga aattggttgg
     3721 ccaaatgcat tttggttgat aatttaggtt tcgtttcgtt ggactcaaac tgccaaatgg
     3781 cttgttccgt taaattaatt ctctgagcta attctgctct acttaaacca tttaacagcc
     3841 gtaattcttt caatacccga ccattaaaca tttacatact ccttactact tttgaccttc
     3901 ttgtttttct attcttggaa taatttcaaa atcttctgtt tccgataatt ctgaaaaatt
     3961 aggaatatct tgatatttag cttcttcgaa atggtacgaa cttgattgaa tatactcaga
     4021 caaatcttga atcaagtgta actgaccagt tcgtgcatca ggcataacaa catctacaga
     4081 tataatattg ttttctaagt ccgcctcata agttaaaatc ataaattttt cgatattcga
     4141 atttttagta gcttgttcaa tttcttgaat catttcatca gaaactaact ccatctgaat
     4201 tggaaaggaa tgactatctt catcattttt gtaggaagaa tgttgattaa gataaagtgt
     4261 attcatttga gcatattcaa ataagtagcc actcttattt ttttgtacca aaggaaattg
     4321 gttcgtaagt cgcttcttac cctttataat taacaatact ttcccatatt tttctgtatt
     4381 tgtttcaaat tctaaatatc cccaagtctg tcctgctaat tgtaatttat actcaaacaa
     4441 atctgctgat gcaaatgcag tatcaatatg attaggtcgc gtccatgcat aaccattcga
     4501 cactatcatt gtctctcttt tttctagacg ttcatctaca taatcttttt gccctttcat
     4561 caaagtatct acaatttttt gtgcctcaag cgaatcaaag agatcctgat tcaacataat
     4621 tcttcctcct ccaaatactt tttaatgaat tatatcattt tcttaaagaa attactacaa
     4681 taattagctt tttcttaaag ttctgtgtca gagtaattta gaaaattata tcttctatag
     4741 taaaatcaat taaaaactga acaaatttat tgggaaattc aaatcgcttt ctgaaaatat
     4801 tttaggaacc gtagtgtaat attccagatt caattcacta taaaactagc ctttctcctg
     4861 caaaagaaaa aggaaagact tcctttcgtg cctttcctct tacttgctac ttgtttgatt
     4921 atttttggta agctactgct tgtttgataa agtcctgaat cggctctcct tggtggagag
     4981 cttttactat tttcgaaccg acgataacac catctgacac cgcattgaag cgttccagat
     5041 cgacttgact agatacacca aaacctgtca agactgggat gtcggccact tgatgaagtt
     5101 gcgccaagtg cttgtccaaa tctgcacggt aattgcctga tttccctgtt accccattga
     5161 tggcaacggc atagacgaat ccctccgccc cttcaatcaa ctctttctgg cgctcaattc
     5221 ctgtggtcaa gcttactaaa ggaatcaagg cgatatctgt atctgccaaa aatggttcta
     5281 caaagttggc atgttcatga ggcaggtctg ggataatcaa gcccttcaca gctgtatcag
     5341 ccagatcttt gacaaagttc tccacaccgt actgaaagag ggggttgaag taggtcatga
     5401 tgaccagtgg aatctctgtt tcaatggttt tcaaggtttc aactaaagcc tgggtagagg
     5461 tcccgtgggc taaactgcgc aagccagctt cttcgataac aggtccatct gcaacagggt
     5521 ctgaaaaggg aatacccact tcaattgcag agacacccaa atcttctaaa aagtgaattg
     5581 tttcagcaag accatccaaa cctttttcgt ggtcaccagc catgatatag ggaacaaaaa
     5641 ttccttttcc agctgcttta atagcattta atttttttgt tagtgtctta ggcatgagct
     5701 tctcccttct ttgctgcatc tgcttccaag cggtccttga cttgaaccac atccttgtcc
     5761 ccacgacctg ataggcagac aatcatagac ttttctggtc caagttcttt ggccaatttc
     5821 accgcaaaag cgatagcatg gctagattcc aaagctggga taatcccttc cacacgagac
     5881 aagagttgga atccttccaa ggcttcttcg tctgtcacag ggacatagct ggcacgttta
     5941 atatcgtggt agtgagaatg ctctggaccg ataccaggat agtccaaacc tgctgagata
     6001 gagaaggctt caagaatttg accatgggca tcttggagca catccatgag ggaaccgtga
     6061 aggacacctg gacgaccctt ggtcaaggta gctgcgtggt gctccgtatc cacaccaaat
     6121 ccagccgctt cagctccata catggctact gactcatctt ctacaaaggg atggaagaga
     6181 ccgatggcat tcgacccacc accaacacag gctactaggg catctggcag attttgtcct
     6241 gtcaagtcac ggtactgttg tttagcctct cgaccaatga cactttggaa gtcacgaaca
     6301 atttctggaa atggatgagg ccccaaggca gaaccaagga tatagtgggt atcatctata
     6361 ttagccaccc atgaacgaag ggctgcattg accgcatcct tgagcacgcg cgaaccatct
     6421 gtcacggcct cgaccttagc tcccaaaagc tccatacgga agacattgag ggcttggcgt
     6481 ttgacatctt cctcacccat gtagatggta cattccatgt taaagagggc cgcagcagtt
     6541 gcagttgcca caccgtgctg accagcacca gtttctgcga taattttctt tttacccatg
     6601 cgtttggcaa gccaaacttg tcccaaggca ttgttaatct tgtgggctcc tgtatggtta
     6661 aggtcttccc gtttgagata aatcttggct ccgccgatat gctgggtcaa gttttttgcg
     6721 taataaagag gagtttcacg tcctacgtac tggcgcaaaa gctggtttaa ttcctcttgg
     6781 aaacttgggt ctgcctgact ttcacggtag gccttctcca actccaaaac tgctgtcatc
     6841 aatgtttctg ggacaaaacg tccgccgaat tttccgtaaa atccatcttt atttggttcc
     6901 tgatatgcca tgctttaccc tctctataaa tcttctaatc ttttcatgat ctttttgtcc
     6961 atctgtctct actccgctcg atacatctac tgcataggga gtaaaatgtt gaattgcttt
     7021 tactacatta tcttcattaa ggccacctgc gataaagaag ggctgtgcta gtccagtcgt
     7081 atccagttga ccccaatcaa aggactggcc acttcctgcc acaggggcat caaagagtag
     7141 ataatctgcc tgagaattgg ggacatgccc atttccatct acctgcacag cctgaatact
     7201 ggcacaaggc aaattctcaa ataaatcatc tgccacctga ccgtgaactt gaaccaagtc
     7261 caagccaact ttgtcaatcg cttccagcag ttctacctga cttggtgaaa caaatactcc
     7321 aacctttttc acatctgcag gaataagctt tgccaactca gctgcctctt ctaaagtcac
     7381 ctgtctttta ctaggtgaaa agacaaaacc gatatagtcg gctcctgctg aaacggctgt
     7441 ttccaccgct tctttggtcg atagtccaca aattttaacc tttgtcaatc tgcaactcct
     7501 tgattctctg ggccacattt tctgcctgca taagagctgt ccctaccaaa attccgttaa
     7561 agtatggggc tagtcgttcc gcatcctgcc ctgtgaaaat ggcagattca gaaatgtaat
     7621 agcgaccttc ctcaaagtaa ggggctaaat ctacactggt ctgcaagtcg acctcaaagg
     7681 tagtcaagtt gcggttgttg accccgataa tctcagcacc aagtctgtgg gctacctcta
     7741 gttcagctaa attgtgagtc tccactaaga cttccagacc aagctctgtc gcgtagtcat
     7801 acagttcctt gaggcgttct tcggacaagg ctgccacaat aagcaagata actgtcgcac
     7861 ctgcattgcg agcgcggatg atttgctttt catctatgat aaagtctttg ttgagcgtcg
     7921 gaatctctac ctgactggaa atttcccgta gataatccaa atgcccttta aagaaaacct
     7981 catctgtcaa caccgaaatc atcactgctc cgttttcttc ataagtctgg gcctgttgca
     8041 caatatccac atcgagattg atatctccca aactagggct agctttcttg acctcagcga
     8101 ttacctgcaa gcggtcctga tgattcttca aaaattctgc caagcgatag gtctggcgca
     8161 gaggctggat ttgctccagc ttcatctgct ccacctcacg cgccttctgc tctaagattc
     8221 gtgctaaaaa ttcctgactc atttttggta ctcctgtaac agtctgagtt tttcaagggc
     8281 cttgcctcta gcaatcactt gacgggccaa ggcaactcct tccttgatgc tatcaatctt
     8341 accattagca tagaaaccaa gaccagcatt caagactgtc gtttccaaga atggacttgc
     8401 ttcgtttttc agaacgctaa gcaaaatttc tgcattttcc tgagcattcc caccacgaat
     8461 atcttccata gcatagcctt ccattcccaa atcctctgga gtaaagcttg acaagctgat
     8521 ttcgccattt tcaagaagtg caatcttggt tgttccgttc aagccagctt catccaatcc
     8581 ttctggtcca gcaaccacga tggcacgttt gcgacccata tttttcaaaa cctgagctgt
     8641 actttctagg agttctggac gactaattcc aagaagctgt gtttctaaag ccattggatg
     8701 aatcagtgga ccagtcaagt tcataatcgt tggaattccc aattccaaac gagctggcat
     8761 gatgtatttc atagctgggt gcatattttt agcgaagaga aagacgattc cagttttatc
     8821 aaagacctta cctagttcag ctggtttgag gtcaagattg attcccaagg cttcgaggac
     8881 atctgcggaa ccagatttag aagaaatcga gcggttacca tgcttggcca tgtgaatacc
     8941 gccaccagcc aagacaaagg ctgcagttgt ggaaatatta aaactgaaag acttgtcccc
     9001 acctgtacca cagttgtcca tggcatcatg aatctcagtt ggaatatgct gggcatgtcc
     9061 tctcatgact tgggcaatgg ctgtgcgttc ttcaggtgtt tcccccttca tcttaagagc
     9121 taagaggaga gaagcaatct gcgcttcagt tacacgccca gttacgatac gctcaatgac
     9181 atccgtcatt tccacacctg ataaattttc aaattttgct agtttttcaa taatctcttt
     9241 catcctagtt tcctcacttt acaaccttct cgataaaatt ccgaatagaa gacaagccgt
     9301 ctggcgttcc aatgctctct ggatggtact ggaagccata aatcggtagg tttttatgtt
     9361 gaatccccat gatggcttgg tcatcagtcg aacgagctgt cacttcaaag tcttctggca
     9421 tttcctcaat caaaatactg tgataacgca tgaccgcacg tccatcctca ataccttgat
     9481 acaaaacaga tggcgcttca aagttgatat tgctctgttt cccatgcatg acttttggag
     9541 ccaaacctag cttaccacca aagacttctg caatggcttg gtggcccaaa caaatcccta
     9601 gaatcggctt cttgcctgca aaatcacgaa tcatgtcttc catctttcca gcatcaactg
     9661 gccaaccagg accaggagaa aagaccagac catctgcttt ttcagcttct tcatacagct
     9721 tggaatcatc atttctcaga acctgaactt ctgcaaaatt cccaatgtat tgggccaagt
     9781 tataggtaaa agaatcatag ttgtcaatca ataaaatcat ggtcttagtt ctccaattct
     9841 agtcatagat tttgctttgt taatggtttc ttggtattcg ttttgggcga tagagtcgta
     9901 gacaatccct gccccagcct gcacataggc tctttgattt ttgagaatca tggttcggat
     9961 ggcgatggcc aaatccatat cacccgtcgc agacaagtag ccgattgccc cagcgtatac
    10021 tccccgtttt tccgtttcca gttcatagat acgtctcatc gctcgaatct ttggtgctcc
    10081 agaaacggtt ccagcaggaa gtgtagcttt caaggcatcc atggcagtga gttctggaag
    10141 caaacgcccc ttgaccacgc tggtcagatg cataacataa cggaagagct ccacttccat
    10201 atacttagtg acttggacac tggtcgtttc agagatgcga ccaatatcat tacgacccag
    10261 gtctactaac attcgatgct ctgctgtttc cttctcatca gagaggagat cagtcgccaa
    10321 ggccttgtct tcttcatccg tagcccctct tggtcgcgtc cctgcaatcg gattggttgt
    10381 cacgatgcca tttttgacag aaaccaaact ttctggactg gctccgatga tttgataatc
    10441 cccaaagtca tataaataga ggtaattgga tggattggtc acgcggagat ttctgtagaa
    10501 gtcaaatgga tttccagtaa cttctgctga gaagcgctga ctgagtacac attggaacat
    10561 atcgccatta cgaatcaagt cgcgagctgt ttccaccatt tcctcaaact tctgaggagc
    10621 gatatggggt ttgaagtcta acggagatag atccaagtct tcaaattcat ttggagcagg
    10681 aatgcgtaat tcctcaagca cttggttcaa ggatttttcc aaggcctctt gactgcgctc
    10741 actataaagt gcattctcta tgacatgtat cttctccttc ttgtggtcaa agaccatata
    10801 gctctcatag acaaagaaat gcatgtctgg cgtcccaatt gtatcctcag ggatttgacc
    10861 aatttcttca taaagcgaaa tcatatcgta acccacaaaa ccaatggctc caccaccaaa
    10921 aggtagctct gagtggtgct gactcttatg aatcacttca taaaggaaat ccaagggatc
    10981 ccgatcaatc acttgaccat tttgatagag aactccattt tcaaacttaa tctcaaaaac
    11041 tggattatag gctaggatag aaaaacgagc tgtttccttg tctctcggaa tactctctaa
    11101 aataacctta tgttgcccct ttaagcgcat ataagccaag attggtgata agacatctcc
    11161 atgaatgatt cgttccattg taatttccct ttcagttcta attctagttc gtggcgactg
    11221 tatgaaaaat ccccacgcaa aataacttgc gtgaggacga aattcgcggt gccacctcaa
    11281 ttataggatt tctcctatct ctcattcctg tctcagatac ctcctgtaac aggctgtgcg
    11341 ataaagggca ctcccttgag aatgatgttt tcttctctcg tttcagatga acccaacttt
    11401 acagctttct ctgcttgttt tcagcaacca caagctctct gtgagagaaa agactgtaat
    11461 ttttccatct attatttttt agcttctagg taatctgcaa tcgcagctag gtccttgcct
    11521 ccacgaccag agatattgat gaggagattt ttatttcgat acacctttat actcttcgaa
    11581 aatctcttca aaccgcgtca acgtcgcctt gccgtaggta tggttactga cttcgtcagt
    11641 tctatctgca acctcaaaac agtgttttga gctgacttcg tcagttctat ccacaacctc
    11701 aaaacagtgt tttgagctga cttcgtcagt tctatccaca acctcaaaac agtgttttga
    11761 gctgacttcg tcagttctat ccacaacctc aaaacagtat tttgagctga cttcgtcagt
    11821 tctatccaca acctcaaaac agtgttttga gcaacctgcg gctagtttct tagtttgctc
    11881 tttgattttc attgagtatt actagctttt ttcgtattag tccagccttt ttgtttgctt
    11941 ttagtagtag gcaaggagct gtagatagaa ctcaagttca tcaaagcgac ttaaggccct
    12001 aataaaagat aaaccaaacg acggatagaa aaaagcccac acacagaata tacttccgtg
    12061 tgagggcgtt ggtaacgcgg tgccacctca attataaagg gactatccct ttacatctct
    12121 gccttgttta acaacaagct gcactgtaag gtgtgcgcac cgaattttca ttgtttcaaa
    12181 ttcattttca aaatcagccc actttcacta cttccaacca cctattcaca atcaccacag
    12241 gctccctgaa gatcaaaaat agttactttt ctgatttgtt gaacttattt taatactttg
    12301 ttttttcttt gtcaagactt ttttacgatt tttttgaaaa tatcattcga atgtgaccat
    12361 gtcttcctta gatcgaacat gaacatgtct cactcgttag aaattggatc caactcaata
    12421 gaaactgaat ggaggctaaa cagaacttat tttagaacac tccatctttt ccactaggat
    12481 tttcaagaat taaacaatac tagaaactct gtctcctaac aaatttagga gaaacttcaa
    12541 cagatgtgac actttcccct ttaataattg ctaaaacacc ttctatcatt tctttagcca
    12601 atttaacata attgggagca attgtagaca aagctggagt ataatactga gaaataggaa
    12661 tattatcaaa tccaatgata gaaatatcat ctggaataag aattcctttc tcatagcacg
    12721 cacgaatcaa gccctgaacc ttttcatctc ctgaaacaaa aataatgtcc ggataatttt
    12781 gggtagtcaa gtgctgcatt gcataagaat aaactgaatc aattgtagat aagccataaa
    12841 tgacttttaa atccataaag taatttttat cattcagaaa agaacgcaca cctctttcac
    12901 gatccttatt aacatgggat tctcctccca taagcaacca catattttta aatttttctt
    12961 cagttacagc tttcatcata tcataagtag cttgaaaatt attattagat acatagacta
    13021 ctccagacgt ttgagattca ccgaaaacaa gaaaaggcat atggttcttc tttaaatact
    13081 gaattctgat atcatctaca ctttcataaa aaacaataac accatctact aggctacctg
    13141 tgcttgatat aattgaatta ctaattgtat cctcctctcc aaaatactca actatagcat
    13201 taacaccaaa ttctttacac gtccgtaaca ctttatctaa cagcgtatga aaccaaatta
    13261 aaggaaaaga gtcgattttt tttacagaaa tcaatatatt tatagcttct tttttagtta
    13321 aattttttgc atacgcattt ggaatatacg acaattcctc tataactttt tgaatcgctt
    13381 gataagtttc ttctttaaca tttactccac cattaataac tcgtgaaact gtttttggag
    13441 aaaaacctga taaacgtgca atatcataaa tagttacctt tttcccattt atatttttca
    13501 tttcagtcct
//
LOCUS       DAVGJJ010000051        13216 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000051 DAVGJJ010000000
VERSION     DAVGJJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.051"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            114..2279
                     /locus_tag="VVE35_001365"
     CDS             114..2279
                     /locus_tag="VVE35_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU3061546.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKFKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2279..3019
                     /locus_tag="VVE35_001366"
     CDS             2279..3019
                     /locus_tag="VVE35_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU3061547.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMKLLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3168..4655
                     /locus_tag="VVE35_001367"
     CDS             3168..4655
                     /locus_tag="VVE35_001367"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU3061548.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(4699..5976)
                     /gene="ftsY"
                     /locus_tag="VVE35_001368"
     CDS             complement(4699..5976)
                     /gene="ftsY"
                     /locus_tag="VVE35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU3061549.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5980..6798)
                     /locus_tag="VVE35_001369"
     CDS             complement(5980..6798)
                     /locus_tag="VVE35_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3061550.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6798..7592)
                     /locus_tag="VVE35_001370"
     CDS             complement(6798..7592)
                     /locus_tag="VVE35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3061551.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7589..11128)
                     /gene="smc"
                     /locus_tag="VVE35_001371"
     CDS             complement(7589..11128)
                     /gene="smc"
                     /locus_tag="VVE35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU3061552.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNMEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNTEQEEIQRLLQEKVDN
                     LEKVDTELLAQQVEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHDLENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHSRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11119..11817)
                     /locus_tag="VVE35_001372"
     CDS             complement(11119..11817)
                     /locus_tag="VVE35_001372"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU3061553.1"
                     /translation="MKELQTVLKNHFAIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12000..12071)
                     /locus_tag="VVE35_001373"
     tRNA            complement(12000..12071)
                     /locus_tag="VVE35_001373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12036..12038),aa:Arg,seq:ccg)
     gene            complement(12135..13121)
                     /locus_tag="VVE35_001374"
     CDS             complement(12135..13121)
                     /locus_tag="VVE35_001374"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU3061554.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
BASE COUNT         3746 a         3030 c         2441 g         3999 t
ORIGIN      
        1 aagaaagaaa aggaaaaatc aatgttatat tttctgacac ggaaaatata aaaatttgcc
       61 tttttttact ttttctgata taatgagaga atattcggaa aaggagacta aaaatgaaga
      121 aaaaatttct agcatttttg ctaattttat tcccaatttt ctcattaggt attgccaaag
      181 cagaaacgat taagattgtt tctgataccg cctatgcacc ttttgagttt aaagattcag
      241 atcaaactta taaaggaatt gatgttgaca ttattaacaa agtcgctgag attaaaggct
      301 ggaacattca gatgtcctat cctggatttg acgcagcagt caatgcggtt caagctgggc
      361 aagccgacgc tatcatggca gggatgacaa agactaaaga acgtgaaaaa gtcttcacca
      421 tgtctgatac ttactatgat acaaaagttg tcattgctac tacaaagtca cacaaaatta
      481 gcaagtacga ccaattaact ggcaaaaccg ttggtgttaa aaacggaact gccgctcaac
      541 gtttccttga aacaatcaaa gataaatacg gctttactat taaaacattt gacactggtg
      601 atttaatgaa caacagcttg agtgctggtg ccatcgatgc catgatggat gacaaacctg
      661 ttatcgaata tgccattaac caaggtcaag acctccatat tgaaatggat ggtgaagctg
      721 taggaagttt tgctttcggt gtgaaaaaag gaagtaaata cgagcacctg gttactgaat
      781 ttaaccaagc cttgtctgaa atgaaaaaag atggtagtct tgataaaatt atcaagaaat
      841 ggactgcttc atcatcttca gcagtgccaa ctacaactac tctcgcagga ttaaaagcta
      901 ttcctgttaa ggctaaatat atcattgcca gcgattcttc ttttgcacct tttgttttcc
      961 aaaattcaag caaccaatac actggtattg atatggaatt gattaaggca atcgctaaag
     1021 accaaggttt tgaaattgaa atcaccaacc ctggttttga tgctgctatc agtgctgtcc
     1081 aagctggtca agccgatggt atcatcgctg gtatgtctgt cacagatgct cgtaaggcaa
     1141 cttttgactt ctcagaatca tactacactg ctaataccat tcttggtgtc aaagaatcaa
     1201 gtaatattgc ttcttatgaa gatctaaaag gaaagacagt cggtgttaaa aacggaactg
     1261 cttctcaaac cttcctaaca gaaaatcaaa gcaaatacgg ctacaaaatc aaaacctttg
     1321 ctgatggttc ttcaatgtat gacagtttaa acactggtgc cattgatgcc gttatggatg
     1381 atgaacctgt tctcaaatat tctatcagcc aaggtcaaaa atttaaaact ccaatctctg
     1441 gaactccaat cggtgaaaca gcctttgccg ttaaaaaagg agcaaatcca gaactgattg
     1501 aaatgttcaa caacggactt gcaaacctta aagcaaacgg tgaattccaa aagattcttg
     1561 acaaatacct agctagcgaa tcttcaactg cttcaacaag tactgttgac gaaacaacgc
     1621 tctggggctt gcttcaaaac aactacaaac aactccttag cggtcttggt atcactcttg
     1681 ctctagctct tatctcattt gctattgcca ttgtcatcgg aattatcttc ggtatgttta
     1741 gcgttagccc atacaaatct cttcgcgtca tctctgagat tttcgttgac gttattcgtg
     1801 gtattccatt gatgattctt gcagccttca tcttctgggg gattccaaac ttcatcgagt
     1861 ctatcacagg ccaacaaagc ccaattaacg actttgtagc tggaaccatt gccctctcac
     1921 tcaatgcggc tgcttatatc gctgaaatcg ttcgtggtgg tattcaggcc gttccagttg
     1981 gccaaatgga agccagccga agcttgggta tctcttatgg aaaaaccatg cgtaagatta
     2041 tcttgccaca agcaactaaa ttgatgttgc caaactttgt caaccaattc gttatcgctc
     2101 ttaaagatac aactatcgta tctgctatcg gtttggttga actcttccaa actggtaaga
     2161 ttatcattgc tcgtaactac caaagtttca agatgtatgc aatccttgct atcttctatc
     2221 ttgtaattat cacacttttg actagactag cgaaacgctt agaaaagagg attcgttaat
     2281 ggcaaaatta aaaattgatg taaatgattt acacaagcac tatggaaaaa atgaagtcct
     2341 aaaaggaatt acgactaagt tctatgaagg agatgttgtt tgtatcatcg gtccttcagg
     2401 ttctggtaag tcaactttcc tccgtagcct caatctttta gaagaagtca ctagcggtca
     2461 catcactgtg aacggctatg atttaactga aaaaacaacc aatgttgacc acgtccgtga
     2521 aaatatcggc atggtattcc aacacttcaa cctcttccct catatgtctg tattggacaa
     2581 catcaccttt gctcctattg agcacaagtt gatgactaag gaagaagctg aggaattggg
     2641 aatgaagttg cttgaaaagg ttggactagc agataaagct aatgccaatc cagatagcct
     2701 atcaggtggt caaaaacaac gtgtggccat cgctcgtggc ctagcaatga atccagacat
     2761 catgctcttc gatgaaccaa cttctgccct tgaccctgag atggttggag acgtacttaa
     2821 cgttatgaag gaattggctg agcaaggcat gaccatgatt atcgtaaccc atgagatggg
     2881 atttgctcgt caggttgcca accgcgttat ctttactgca gatggcgagt tccttgaaga
     2941 cggaacacct gaccaaatct ttgataaccc acaacaccct cgtctgaaag agttcttaga
     3001 taaggtctta aacgtctaaa ctcaaactgt aaggatttcc ttgcagtttt tctacttcgt
     3061 attggaattt ttgatttttc ggaaaattat gttagaatta agtttatgaa atgaggtttc
     3121 ctcataccta gcaagactag gaataaaaat agaaattagg tagctagatg tcatctaagg
     3181 ttattgttac aattttcggt gcgagtggag acctggctaa acgcaagctc tacccttccc
     3241 tttttagact atataaatcc ggcaatcttt ccaagcactt tgccgttatt ggaactgccc
     3301 gtagaccttg gagtaaggaa tattttgaat ctgtagttgt cgagtccatc cttgatttgg
     3361 cagatagtac cgagcaagcc caagaatttg ctagccactt ctactatcaa agccatgatg
     3421 tcaatgattc ggaacattat attgctttgc gtcaattaca agctgagctt aatgaaaaat
     3481 accaagctga acacaataag ctcttcttct tgtctatggc acctcagttc tttggaacca
     3541 ttgccaaaca cctcaaatct gaaaacattg tcgatggcaa aggttttgag cgcttgatcg
     3601 ttgaaaaacc atttggtaca gattacgcaa ctgcaagcaa gttgaatgac gaactcctag
     3661 caacatttga cgaagaacaa attttccgta tcgaccatta tcttggtaag gaaatgatcc
     3721 aaagcatctt tgcagttcgc tttgcaaact tgatttttga aaacgtttgg aacaaggatt
     3781 ttatcgacaa tgttcaaatt acctttgcgg agcgcttggg tgtagaagaa cgtggtggct
     3841 actatgacca atccggtgcc ctccgtgaca tggtccaaaa ccacactcta caacttcttt
     3901 cgcttctcgc catggacaaa ccagcaagct tcacaaaaga cgagattcgt gctgaaaaga
     3961 ttaaggtctt taaaaacctc tatcatccaa ctgatgaaga actcaaagaa cactttatcc
     4021 gtggacaata ccgctctggt aagattgatg gcatgaaata catctcttat cgtagcgagc
     4081 caaatgtgaa tccagaatca acaactgaaa cctttacatc aggtgccttc tttgtagaca
     4141 gcgatcgatt ccgtggtgtt cctttctttt tccgtacagg taaacgactg actgaaaaag
     4201 gaacccatgt caacatcgtc tttaaacaaa tggattctat ctttggagaa ccacttgctc
     4261 caaatatttt gaccatctat attcaaccaa cagaaggctt ctctcttagc ctaaatggga
     4321 agcaagtagg agaagaattt aacttggctc ctaactcact tgattaccgt acagatgcga
     4381 ctgcaactgg tgcttctcca gaaccatacg aaaaattgat ttatgatgtc ctaaataaca
     4441 actcaactaa ctttagccac tgggatgaag ttggtgcgtc atggaagttg attgaccgta
     4501 ttgaagagct ctgggccgaa aatggtgccc cacttcatga ctataaagct ggaagcatgg
     4561 gacctcaagc cagctttgac ctacttgaaa aattcgatgc caaatggact tggcaaccag
     4621 atatcgccta tcgtcaagat ggtcgtttcg aataaaaaaa tcctgcaagt ttgtaccttg
     4681 caggattttt gcttctgatt agattaagcc ttccaagaga cctttcataa agttttctga
     4741 gttaaactct ccaatatcat cgattttttc accaaaacca atcaatttta caggaatatt
     4801 gagttcttca cgaatggcta gaaccacacc tcctcgagca gttccatcaa tcttagtcaa
     4861 aacaattccc gttaaaggtg tgattttcga aaattctttg gcctgtacta gggcattttg
     4921 acctgttgat gcatcaagtg ccaagaaggt ttcatgtggt gcttctggca caacacgttt
     4981 gataatacga ccaatctttt ccaactcagc cataaggtta tccttatttt gcagacgacc
     5041 agcagtatca atcatgagaa tatcgatacc ttcagccacg gcacgttcca taccatcaaa
     5101 gaccacgctg gctggatcag ctttttcagg tccagttact actggaacat ctactcgtcg
     5161 gccccattca gctagctgag ctactgcacc cgcacggaag gtatctgctg caaccagcat
     5221 gaccttctta ccagctcgtt tgtagcggtg ggctagtttt ccgatagaag ttgttttccc
     5281 aacaccattc acaccaacaa agagcataac tgtcaagtta tcttggaagt ggatgctttc
     5341 atcgtagcta ccatcctttt cataaagctc aaccaatttc tcaatgatga cacgacgaag
     5401 tgcatcaggt ttcttggcat tttcaagctt ggcttcgtaa cgtagttcct ccgttaagtt
     5461 agaagcgact tggacaccaa catcactcat aatcaacagt tcttccagtt cctcgaaaaa
     5521 ttcttcgtca acagagcgga agttagcaaa gaaggcattc aagcgggcac cgaaacctgt
     5581 gcgagttttc ttaagactgc ggtcatattt ttcctgaaca gtttcttctg tttgaggcct
     5641 ttctgcttca agaatttcag aattattttc ttctacagtc tctagaaact cagtattccc
     5701 ttcttctgag tgtggaaatt cttcgacttc tttttgagaa acttctacaa ctggctctaa
     5761 atccagactt tcttcaactg tgtcttggat ttcctcttct tggaacacag cttgttcaac
     5821 aatttcaacc tctgcttctt cctgagaaac ttcctcaact tctgtgaagg taggatcaac
     5881 atcttcagac aaatcaagat tttccagagc ttcttttaca acttcttcga ttttaggttc
     5941 ttcttttttt ccgaatagac ggtcaaacaa tcccatatct tagttctcct ttagcacata
     6001 ttcttcgata gcccaggcga cagcttcctc atcgttggtc atcggcgtca ctacatttgc
     6061 ggctgccttt acttcaggaa cagcgttttg catagcaaca ccaagacctg cccattcaat
     6121 catagagagg tcattggcct cgtcaccaca agccatcact tgactttggt cgattccaag
     6181 atggctgatt agttttgcca aacctgttgc tttatgaaca ttctttggtg accattctag
     6241 caacatttca cgtgatttaa agatttcata ttggtcaaac aattctggag aaatcttctg
     6301 aatggctgca tccaagggtt cttgagcaaa ggcagtcacg catttgttgt aggtcatttg
     6361 actagataag tcttcaaagt ccactggaac aaaggtcaaa gctggattga atttggcata
     6421 aagactttct tggtccgatt ggatttgata aactgttcct tctgagatgg catcaagagg
     6481 cagtgataat ttctctgttt cttcatacaa acgtgccaca tcatcatatg aaaagactgt
     6541 tttatcaagg atttctcctg tatttttctg aactaatcca ccattaaaag taatggtata
     6601 ctcatcttcc tgaccgtcag tccctaactc atggagaaag aaatccatag cttttaaggg
     6661 acgaccagtt gtcaatacga ccttgatacc acgatcacgc gcagctttca aggtttcctt
     6721 ggtacgatcc gtcagccttt tatcagtagt cagcaaggtc ccgtccaagt ccaatgcaat
     6781 caattttata tctgccatta taagccctcc atataagcta taaccgaccg ttccttatgg
     6841 tgaccaatca cagtctttgc taattctaaa atttcaggtc gtgcattttc aggagctaca
     6901 ggatgtccca caacctgcat catatgtaag tcattaagat tgtctccaaa agccatgacc
     6961 tgatccattg tgataccaag ttttttagct aattcaacaa tagctacacc cttatcgaca
     7021 tagtccagaa caatatcaat agattcaaag ccagttgtca tagccttgac accagggaca
     7081 ttatcattga cccaagcttc accagcttct agcgtttctt ctgtgaagtt ggttgtaaat
     7141 ttgaaaatgt catctgtgat atcttccaaa ctcgctactt tttggatatt ttcattataa
     7201 tgctgactca ctttcaaata ggtctcatca accgtatcta gaacatatga acccttctta
     7261 cccgtcaaga gcagtttatt gatatctaca taaggtgacg ttttcagctt ttcaaaagtt
     7321 gccagataaa agtcacgaga catagtcgct tcatacaagt cctgaccttg atactctacc
     7381 aaactgccat tttccgcgat gaaaataatg tcatcacgaa caccagcaaa taatttttcc
     7441 agagacagaa atccccgacc cgaagccacc gcaaagtaaa tccctttttc cttgtaggaa
     7501 gccaagagag acttgagacg atccatatca aagcgtccat tcccatctag gaaggttccg
     7561 tccatatccg ttgctactag tttaattgtc atccttcaat actttctaaa tcttttaact
     7621 taaccgaaac aatttttgag acacctgatt cttgcatggt cactccatag atggaatcag
     7681 ccgccgccat ggttccctta cggtgggtta cgacgataaa ctggctgtcc ttgtcaaatc
     7741 gattgaggta atccccgaaa cgcttaacat tagcttcgtc cagcgcagcc tctacctcat
     7801 ccaaaataac aaatgggata gtcttaacac ggataatgga gaagagcaag gcaagagccg
     7861 atagggcttt ttccccacca ctcatgaggt taagagactg gattttctta cctggaggtt
     7921 gaacagaaat ctcaacccca gctgtcagta agtctccctc agtcaaaatc aggtcagcct
     7981 gacctcctcc aaacatctgc ttaaaggtca ctttaaagga ctcacgaatt gcctcaaagg
     8041 ttgatttaaa gcgttccttg acctcatcat tcatctctgt aatggtctca aggagcagat
     8101 ttttcgctga caaaatatca tctcgctgac tatttagaaa gtccagacgg ctgtgaactt
     8161 cttcgtactg gtcaatagct tccaaattga caggacccag tgagcgaata gccttctcta
     8221 aatcctgcac ctcttgctct gccagattga gattttccaa atcatgtgcc ttttctagag
     8281 cttcagtata gctaatctga tactggtctg ttaattgact ttgtagatgg cgcaagcgct
     8341 cgctgacctt ttctttcttg gcttcagcac gagtttgctt gcgaatccac tcttcattct
     8401 gctggcgagc ctgatccaaa tgactagcaa tatcatccag ctgaccttct atatcatcca
     8461 attcaaactg cttgcgaatc aaaccttgtt ggagatttgt tttctgagtt ttggcctctt
     8521 ctacctgctg ggctagaagt tctgtgtcaa ctttctcaag attatcaacc ttttcttgga
     8581 gaaggcgctg aatttcttct tgctcggtat tcagattgtc caattccttg cctaaacgct
     8641 caatatcagc tacttcataa cgtttttgcc cttgcagttc tgtcttaagc aagcgagctt
     8701 gcgccacctc ttcctgcaag ttttgatagc gttcttggat ggcgttttta ttagacttaa
     8761 tttcttcaat ctcagcttcc atattttgct tttcactggc gatagtagca aggctctctt
     8821 ggcatttttc cttatccgct tgccaatctc cctcggaaag acgatctatt tcctcttctt
     8881 ggagtttcca aagagtttcc agttcttcaa cttgctgact agtttgctga taagcgagga
     8941 acaagccttg ctcctgaata cgtgcctgct ctccttgaga tttaatagct tctaatgact
     9001 cggtcaatct ggccatctca tcttgcaagg tcttcaaagc cacttcttct gaacccaagc
     9061 ttgcttcatc tgcagcaatt tctttttgta attgctccag ttctggcttg atgaagatgc
     9121 tgttgttctg acgattggct ccacctgcat aggaaccacc cgtgcgtaat tctgtcccat
     9181 ccaatgtcac catacgaacc tgataacgaa cttggcgagc tgctgcacgc gcatgttcta
     9241 cggtatcaaa gatagccgtc gtagctagca agttcttgaa aatggcttcc agtctagtat
     9301 caaaagtcac caactcatct gccatcccaa ggaaacctgg gcttacagcg atagcatctt
     9361 ggttctgact agaaatcgta cgcgccttga tagtggtcaa aggaagaaag gttgcacgac
     9421 cggctctgtt ccgtttaagg aagtcaatag ccttggttgc cgactcttca tcttctacga
     9481 tgatatgttg gctactcgct ccaagcgcaa tttctagggc agtttgataa tgcatatcaa
     9541 aggtcagatg ctcactgact gcaccaataa tcccacctag gcgatctttt tcttggagaa
     9601 cactcttaac acctgcataa aagttactat gatttctcag gatattttcc aaactttgag
     9661 ctctggcctg cttgtttttg agattatcca gacggtcaaa gagttggctt tgttgagctt
     9721 gataggaagt tttctgctcc tcttgctcct tggcaatagc ttggtagtca gccaataatt
     9781 tctgaacctg ctccttggca gtttcaagct cttccttttg ctgactagcc ttctctttag
     9841 ctatagccaa ttgctctttc agcttttcta gttgatctgc ttgtttttga gaaagctgac
     9901 gactattttc caactcattc tcaatacggg tcaactggtt tgagacatcc gcttcttctt
     9961 gtaaaagagc tacaaagcgt tcacgtaaga gctcaatcat ctgatcagga tcgtctgaga
    10021 aagccagcaa ttcagcttct aaacgattga gtttttgatt attttggact agatttccct
    10081 ctaacagagc taaagagctt tctttatcat acttttcttt gctgagtgaa tttctcttat
    10141 cctccaaagc agccaaacga gcttgtgcct cctgttgatt cagggctact tgctcggact
    10201 ccagtttcga tagggctaat tttctttcta aatcactaat cagactagtc aagtccatca
    10261 aactgccttg gtctttggcc atttcagcct gtaaatcttg gcgttgcttt ttaagagttt
    10321 gattttcttc ttctaatttt tcacgctttt ggtaataact catcaagagt tcttgaacct
    10381 gagtcaactc ttcttctgtc gactctagtt cagccttatt ttccttgatt tgagcaacca
    10441 gaacatctaa ataaatagcc ttacgttgtc cttccaagtc taaaaactta cgggcattct
    10501 cagcttgctt ctcaagaggc ttgatttgat tatccaactc gtagataatg tcctctaagc
    10561 ggtccagatt atcctgagtt tgctgcagtt tactctcggt ttcttttctg cgagtcttgt
    10621 attttaaaac tccagcagct tcttcaaaaa tagctcgtcg ttcctcaggc ttagaattaa
    10681 aaatctcctc aaccttccct tgggaaataa tagagaagga atctcgtccc aatccagtat
    10741 ccaagaagag gtcatgaata tcacgcagac ggactttctt gccgtcaatc ttgtattcgc
    10801 tatctccact acgatagata tggcgttcca ccctgatttc ttgacctgca tccttgataa
    10861 atccgtcatg attatccaga gtcacaacta cagaagcata attgagcggt ttgcgacttt
    10921 cggttccagc aaagatgaca tccggcatct tgcccccacg gagactcttg acactagact
    10981 cccccaaggc ccaacgcaga ctttctgtaa tattggactt tccagatcca ttgggtccaa
    11041 caactgccgt cacaccttgg tcaaaaacga ccttggtctt atcagcaaaa gacttgaacc
    11101 cctgaatttc gatttccttt aaatacatga atccagcccc ttctcaacgg catttttggc
    11161 agcttcctgc tctgctaatt tcttagaacg accttggcct tgaccgatgc tcttaccttc
    11221 aacaagaact tctacatcaa agaccttatc gtgagcaggc cctgtttcag aaatcacctg
    11281 ataacgaata gccacatcac cattgacctg aagcaactct tggagatagg ttttatagtc
    11341 tgtaatcatc tcaaactcgc ctgcttcaac cttaggaatc atgacttgat agataaattc
    11401 cttgaccttg gccacatcct tatccaaaag aagggcacca agaaaggctt caaaggcatc
    11461 accaagaatg gtgtcacgat tgcgaccacc tgatttttct tcccctttac ccaacttgat
    11521 aaactggtca aactggcaat cacgcgcaaa accagctaaa ctctcctcac ggacaatcat
    11581 agcacggagt tttgataggt caccttcagg ctttttagga tattttttat atagatattc
    11641 tgaaatcaat aactgtagaa cagcgtctcc taaaaattcc aagcgttcat tgtgtgaaat
    11701 ttttaagagg cggtgctcat tggcataact cgtatgagta aaggcagttt ccagtaactt
    11761 tttgtctgca aattcgattg caaaatgatt ctttagtaca gtttgtaatt ctttcatacc
    11821 aacctctttc taactgataa tagtcctttt tattatatca aaaaaagccc cctgagtcac
    11881 tctaaaacgg gactggaaag catttgggaa ttctttagac agagattctc agttttagcg
    11941 gcaaatttgg gtcaggataa agaaaaaagc cctattaaag gctttttagg atgtttacat
    12001 ccaccctgag ggaatcgaac ccccatctca agaaccggaa tcttacgtga tatccattac
    12061 actaagggtg gaaacttgtt ttattataac agaaatttgc tctaataaca agttttttgg
    12121 tcaaagaccc cgtcttagtg ggaagcatcc ccattccaga tggagttttt cacgatcaca
    12181 taatcaacgt gtttaaggtc agcaacctga cgtccacctg cataagaaat agcactttga
    12241 aggtcttgtt ccatctcagt taaagtgtct tgcagatgac ctttagcagg aagcaagata
    12301 cgtttgcctt ccacattttt gtaagcacct ttttgatatt gtgaggctga accataatat
    12361 tctttgaact gttcaccatc gacttcaatc gttttccctg gactttcaat gtgtcctgca
    12421 aagagggaac caatcatgat catgctagca ccgaagcgga tagacttggc aatatcaccg
    12481 tgagtacgaa ttcctccatc agcgataatc ggtttacgcg cagccttggc acaccagcgt
    12541 agagcagcca actgccaacc acctgtacca aaaccagtct taaccttggt gatacaaacc
    12601 ttaccaggac cgattccgac cttagtagca tccgcaccag cattttccaa ttcacgcaca
    12661 gcttctggtg ttcccacatt tccagcaatt acaaaggtat ctggcaattc tttcttgatg
    12721 tgttgaatca tagaaatcac gctatccgca tgaccatgag caatatcaat agtgatatac
    12781 tcaggagcat cagtcttgag ctgtctaacg aaatcatact catagtcctt aacaccgaca
    12841 gatatggaag caatgagccc ttgatcgtgc attcgtttaa taaaaggaat gcgtcctgcc
    12901 tcatcaaaac ggtgcataat gtagaagtaa ccacctttag ccagttgctc tgctacattt
    12961 tcatccaaaa tcgtctgcat attcgctggc acaacaggta gtttaaaggt gtgatttcct
    13021 aaagtgacac ttgtatccgc ttctgcacgg cttttaatga cacatttatt tggaatcaat
    13081 tgaatatctt cgtaatcaaa aattggaaat tcatttaaca tatcgatgtc tcgtttcttt
    13141 tgtaatgacc tacctatgct cttgcatcac tacgcctttt ccgacgtttc cttaatttat
    13201 tataaaccaa agtaca
//
LOCUS       DAVGJJ010000052        13084 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000052 DAVGJJ010000000
VERSION     DAVGJJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.052"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            496..1611
                     /locus_tag="VVE35_001375"
     CDS             496..1611
                     /locus_tag="VVE35_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3061555.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVIKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLP
                     QQPYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGI
                     SGGQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTI
                     AERTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            1624..2973
                     /locus_tag="VVE35_001376"
     CDS             1624..2973
                     /locus_tag="VVE35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU3061556.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3143..3850
                     /locus_tag="VVE35_001377"
     CDS             3143..3850
                     /locus_tag="VVE35_001377"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU3061557.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKNQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4052..7777
                     /locus_tag="VVE35_001378"
     CDS             4052..7777
                     /locus_tag="VVE35_001378"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU3061558.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANNAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHSLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            7870..9312
                     /locus_tag="VVE35_001379"
     CDS             7870..9312
                     /locus_tag="VVE35_001379"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU3061559.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FYDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            9349..10371
                     /locus_tag="VVE35_001380"
     CDS             9349..10371
                     /locus_tag="VVE35_001380"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU3061560.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            10368..10913
                     /gene="purN"
                     /locus_tag="VVE35_001381"
     CDS             10368..10913
                     /gene="purN"
                     /locus_tag="VVE35_001381"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU3061561.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFSVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            10997..11506
                     /locus_tag="VVE35_001382"
     CDS             10997..11506
                     /locus_tag="VVE35_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU3061562.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            11531..13078
                     /gene="purH"
                     /locus_tag="VVE35_001383"
     CDS             11531..13078
                     /gene="purH"
                     /locus_tag="VVE35_001383"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU3061563.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
BASE COUNT         3659 a         2554 c         3196 g         3675 t
ORIGIN      
        1 cagtattact aattgggata acaaattatg cgagatttaa aaatttaaga ttgacagtag
       61 tgttttctgt accattttta tttaatgcta taatgtctat tataaatagt ttttagaaat
      121 actaaaattt ttttctttac ttaataatga tatgaaatca tttcggtttt gttaacttgt
      181 aattcttata ttttctaggt agaacgtttg ttctgcctat ttttttatcc aaaaagtgca
      241 gttgggaggg agataggctc atttgggaag gaagtccagt ttttgtttag tgattggggt
      301 aagatagttg ttatcagatg agtttatact cttcgaaaat caaattcaaa ccacgtcaac
      361 gtcgccttgc cgtatatatg tgactgactt cgtcagtcct atctacaacc tcaaaacagt
      421 gttttgagca gcctgcggct agtttcctag tttgctcttt gattttcatt gagtattagg
      481 gaaaaggaga tgaatatgaa atttgggaaa cgtcactatc gtccgcaagt ggatcagatg
      541 gactgcggtg tagcttcatt agccatggtt tttggctact atggtagtta ttattttttg
      601 gctcacttgc gagaattggc taagacgacc atggatggga cgacggcttt gggcttggtc
      661 aaggtggcag aggagattgg ttttgagacg cgagccatta aggcggatat gacgcttttt
      721 gacttgccgg atttgacttt tccttttgtt gcccatgtgc ttaaggaagg gaaattgctc
      781 cactactatg tggtgactgg gcaggataag gatagcattc atattgccga tccagatccc
      841 ggggtgaagt tgactaaact gccacgtgag cgttttgagg aagaatggac aggagtgact
      901 ctttttatgg cacctagtcc agactataag cctcataagg aacaaaaaaa tggtctgctc
      961 tcttttatcc ctatattagt gaagcagcgt ggcttgattg ccaatatcgt tttggcaaca
     1021 ctcttggtaa ccgtgattaa gatgatggtt aatttttacg acccaagtca aggggagatt
     1081 agtctgggtg gtgtcaatct caatcagatt gataaaaaag ccctgcgcca gtacatcaac
     1141 tatctgcctc aacagcccta tgtctttaac ggaacgattt tggagaatct tcttttggga
     1201 gccaaggagg ggacgacaca ggaagatatc ttacgggcgg tcgaattggc agagattcga
     1261 gaggatatcg agcgcatgcc actgaattac cagacagaat tgacttcgga tggggcaggg
     1321 atttcaggtg gtcaacgtca gagaatcgct ttggcgcgtg ctctcttgac agatgcgccg
     1381 gtcttgattt tggatgaggc gactagcagt ttggatattt tgacagagaa gcggattgtc
     1441 gataatctca tggctttgga caagaccttg attttcattg ctcaccgctt gactattgct
     1501 gagcggacag agaaggtagt tgtcttggat cagggcaaga ttgtcgaaga aggaaagcat
     1561 gctgatttgc ttgcacaggg tggcttttac gcccatttgg tcaatagcta gaaagaggag
     1621 aggatgaaac cagaattttt agaaagtgcg gagttttata atcgtcgtta ccataatttt
     1681 tccagtagtg tgattgtacc catggccctt ctgctcgtgt ttttacttgg ctttgcaact
     1741 gttgcagaaa aggagatgag tttgtccact agagctactg tcgaacctag tcgtatcctt
     1801 gcaaatatcc agtcaactag caacaatcgt attcttgtca atcatttgga agaaaataag
     1861 ctggttaaga agggggatct tttggttcaa taccaagaag gggcagaggg tgtccaagcg
     1921 gagtcctatg ccagtcagtt ggacatgcta aaggatcaaa aaaagcaatt ggagtatctg
     1981 caaaagagcc tgcaagaagg ggagaaccac tttccagagg aggataagtt tggctaccaa
     2041 gccacctttc gcgactacat cagtcaagca ggcagtctta gggctagtac atcgcaacaa
     2101 aatgagacca tcgcgtccca gaatgcagca gctagccaaa cccaagccga aatcggcaac
     2161 ctcatcagtc aaacagaggc taaaattcgc gattaccaga cagctaagtc agctatggaa
     2221 acaggtgctt ccttggccag tcagaatcta gcctactctc tttaccagtc ctacaagtct
     2281 cagggcgagg aaaatcccca aactaaggtt caggcagttg cacaggttga agcacagatt
     2341 tctcagttag aatctagtct tgctacttac cgtgtccagt atgcaggttc aggtacccag
     2401 caagcctatg cgtcagggtt aagcagtcaa ttggaatccc ttaaatccca acatttggca
     2461 aaggttggtc aggaattgac ccttctagcc cagaaaatct tggaggcaga gtcaggtaag
     2521 aaggtacagg gaaatctttt agacaagggg aaaattacgg cgagtgagga tggggtgctt
     2581 catcttaatc ctgagaccag tgattctagc atggttgcag aaggtgccct actagcccaa
     2641 ctttatccat ctttggaaag agaagggaaa gccaaactca cagcctatct aagttcaaaa
     2701 gatgtagcaa gaatcaaggt cggtgattct gttcgctata ctacgactca tgatgccggg
     2761 aatcaacttt tcctagattc aactattaca agtattgatg cgacagctac taagactgag
     2821 aaagggaatt tctttaaaat cgaggcggag actaatctaa cttcggagca ggctgaaaaa
     2881 cttaggtacg gggtggaagg ccgcttgcag atgattacgg gcaagaaaag ttatctacgt
     2941 tattatttgg atcaattttt gaacaaagag taatgttcgt gtttttagag ttaaataatt
     3001 tttaaactgt gagaaagatt cttcttgcag ttttttcttt acaatttttg aaaaacatct
     3061 actatttatt cggttaagtt cttgtgtttt ttggtttttt gtggtaaaat gtgctcaagt
     3121 aatacgaaag gcgaacttta aaatgtcaaa acaattgatc tattcgggaa aagctaaaga
     3181 tatctataca actgaggatg aaaatcttat tatttcaact tacaagaacc aggcgactgc
     3241 tttcaacggt gtcaagaagg agcagattgc aggtaaggga gtcttgaata atcagatctc
     3301 atcttttatt tttgagaaat taaatgcggc tggtgtggcg actcactttg tggagaaact
     3361 ttcagacacg gaacaactca ataaaaaggt taagattatt cctttggaag tcgtgctccg
     3421 caactatact gctggttcct tttcaaaacg ttttggtgtg gatgagggaa tcgccttgga
     3481 gactccgatt gtcgaatttt actacaaaaa tgatgatttg gatgatccat ttatcaatga
     3541 tgagcatgtg aaattcctac agattgcggg tgaccagcag attgcctact tgaaggaaga
     3601 aacgcgtcgt atcaatgaac tattgaaagt ctggtttgct gagattgggc ttaaattgat
     3661 tgactttaag ctagagttcg gttttgacaa ggatggcaag attatcttgg cagacgaatt
     3721 ttcaccagat aactgccgct tgtgggacgc tgatggcaac cacatggata aggatgtttt
     3781 ccgtagagga ttgggagaac taaccgacgt ttatgagatt gtttgggaaa agttgcagga
     3841 attgaaataa tctgtttgca acggaaaacc ttcgtctctc aactaaaagg actcaggctg
     3901 aaaaggtccc ccagaccttt tcactctgta gagaactagg tgaactaaca gatgtttacg
     3961 aaattgtctg ggaaaagttg cagggtttaa aataacaacc tcaaggctgt ttgggaatat
     4021 tgcaagagct gaaataaagg aataagaatt gatggataaa cgtatttttg ttgaaaaaaa
     4081 ggctgatttt caggtcaagt cagagagttt ggttagagag ctccagcaca acttgggact
     4141 gtcaagcttg aaaagtattc gtattgtgca agtatatgat gtatttgact tggctgagga
     4201 cttgtttgca cctgcagaga agcacatttt ctctgagcag ggaaccgacc atgttttaga
     4261 tgaagtatct gtgcaggcgg atcttgctaa caatgctttc tttgccattg aaagtctgcc
     4321 agggcagttt gaccagcgtg cagcttcgtc acaggaagcc ttgcttttgt tgggaagttc
     4381 gagtgacgtg acagtcaaca cagcccaact ttacttggtg aataaagata ttgatgcgac
     4441 tgagttggaa gctgtcaaaa actacctgct caatccagtt gattctcgtt tcaaggatat
     4501 cacgacaggg attgccaagc aggagttttc agagtcagac aagaccattc caaaattgac
     4561 tttctttgaa agctatacag cagaagactt tgctcgctac aaggccgagc aagggatggc
     4621 catggaagtg gatgatttgc tctttatcca agactacttt aagtcaatcg ggcgcgtgcc
     4681 aactgagact gaactcaagg ttttggacac ttactggtct gaccactgcc gtcatacgac
     4741 ttttgagaca gagttgaaac acatcgactt ttcagcttct aaatttcaaa agcaattgca
     4801 gtcaacctat gacaagtata ttgccatgcg cgaggagtta ggtcggtctg aaaaaccaca
     4861 aaccttgatg gatatggcga ctattttcgg tcgttatgag cgtgctaacg gacgattgga
     4921 tgatatggaa gtctctgacg aaatcaatgc ctgctcagtt gaaattgaag tggatgttga
     4981 tggtgtcaag gaaccttggc tcctcatgtt taaaaatgaa acccacaacc atccaacaga
     5041 aattgagcca tttggtggag cggctacctg tatcggtgga gctattcgtg atccgttgtc
     5101 aggccgttcc tatgtttacc aagccatgcg tatttcaggt gctggtgata ttacagcacc
     5161 gatttcggaa actcgtgctg ggaaattgcc acaacaagtc atttctaaaa cagcagctca
     5221 tggttattct tcatatggta accagattgg gcttgccaca acctacgttc gtgaatactt
     5281 ccacccaggc tttgtagcta aacgtatgga acttggtgcc gttgttggtg cggctcccaa
     5341 gggcaatgtt gtccgtgaaa aacctgaagc aggtgatgtg atcatccttc tcggaggcaa
     5401 aacaggtcgt gatggtgtcg gtggtgcgac gggctcttct aaggttcaaa cagttgagtc
     5461 tgtagagact gctggtgctg aggttcaaaa aggaaatgcc atcgaagaac gcaagattca
     5521 gcgcctcttc cgtaatggca atgtcactcg tctgatcaag aagtccaatg actttggggc
     5581 aggcggcgtc tgtgtggcta tcggtgaatt ggcagacggt cttgaaatcg acctcaacaa
     5641 ggtgcctctt aaataccagg gcttgaatgg tacagaaatt gccatctctg aatcacaaga
     5701 acggatggcg gtcgtggttc gtcctgaaga tgtggatgcc ttcgttgccg aatgtaacaa
     5761 agaaaatatt gatgctgttg tggtggcgac agtaactgaa aaaccaaatc ttgtcatgca
     5821 ctggaatggt gagacaatcg ttgacttgga gcgtcgtttc cttgacacca atggtgtgcg
     5881 cgtggttgtc gatgccaaag ttgtggacaa ggatgtcaaa ctcccagaag agcgtcaaac
     5941 atctgctgaa acactggaat cagataccct tacggttcta tctgacctca accatgcaag
     6001 tcaaaaagga ttacagactg tctttgactg ctctgttgga cgctcaacgg ttaatcactc
     6061 acttggtggt cgttaccaac tcacaccaac tgaggcatct gtgcagaaat tgccagttca
     6121 acacggtgtg actcatactg cgtcggtcat tgctcaaggt ttcaacccat atgtagctga
     6181 atggtctcca taccacggtg ctgcttacgc ggttatcgaa gcaactgctc gtttggtggc
     6241 tgctggtgcc aactggtcta aggctcgttt ctcttaccaa gaatatttcg agcggatgga
     6301 caagcaagca gagcgttttg gtcagccagt agccgccctt ctaggctcta ttgaagctca
     6361 gattcaactt ggcttgccat ctatcggtgg taaggactcc atgtctggta cctttgaaga
     6421 attgaccgtt ccgccaacct tggttgcctt tggggtgacg acggcagata gccgtaaggt
     6481 gctctctcca gaatttaaag ctgttgggga aaatatctac tacatcccag gtcaagccct
     6541 ctctgcagag attgattttg acttgattaa gaaaaatttt gctcagtttg aagcaagcca
     6601 agctgaccat aaagtgacat ctgcatcagc tgtcaaatac ggtggtgtag ttgaaagttt
     6661 ggctcttgct acctttggaa actatattgg tgcagaggtg accttgcctg aacttaaaac
     6721 agctttgaca gctcaattag gcggctttgt cttcacatct cctgaagaaa ttgctggagt
     6781 agagaagatt ggacaaacga aagcagactt tacactgact gtcaacggtg tgaagctaga
     6841 tggacacaag cttgacagtg catttcaagg gacattggaa gaagtttacc caacagaatt
     6901 tacccaagcg aaagaactag aagaagtacc agctgtggca tcagatgttg tgattaaagc
     6961 caaagaaaag gttgaaaaac ctgtggttta catcccagtc tttccaggaa ccaactcaga
     7021 atatgattca gctaaggcct tcgaaaaaga aggtgcagag gtcaatttgg tgccattcgt
     7081 gaccttgaat gaagaagcta ttgtcaagtc agttgaaact atggttgaca atatcgacaa
     7141 gactaatatt ctcttctttg ccggtggatt ctcggctgcg gatgaaccag atggttcagc
     7201 taagtttatc gtcaatatcc tgcttaatga aaaagtgcgt gtggctattg atagctttat
     7261 cgcccgtggt ggcttgatta tcggtatttg taatggattc caagccttag tcaaatcggg
     7321 tctcctacca tacggaaact ttgaagatgc taacagtact agtccaaccc tcttctacaa
     7381 tgatgccaac caacacgtgg ccaagatggt ggaaactcgc attgccaata ccaactcacc
     7441 atggttggct ggtgtgcaag tgggcgatat ccacgctatt cctgtttcgc acggtgaagg
     7501 gaagtttgtc gtgacggctg aggaatttgc agagctccgt gacaatggac aaattttcag
     7561 ccaatacgtt gactttaacg gtaaaccaag tatggattct aagtacaatc cgaatggttc
     7621 tgtccatgcc atcgaaggaa ttaccagcaa gaatggtcaa atcatcggta agatgggcca
     7681 ctcagaacgt tatgaggatg gtcttttcca aaatatccca ggcaataaag accaacacct
     7741 gttcgcatca gcggttaaac atttcactgg aaaataagac ttacagattt tctaatagat
     7801 agtatcagta atgtaaaagt catgtaaatc tagctcttga tgattacaaa tgaaaattag
     7861 gtataaaaaa tgacatacga agtaaaatct cttaatgaag aatgtggtgt tttcggtatt
     7921 tggggacatc cagatgctgc taagttgacc tattttggac tccacagtct tcaacatcgt
     7981 ggtcaggagg gggcaggaat cctctccaat gatcaaggac aactgaagcg ccatcgtgac
     8041 atggggcttt tatcagaagt tttcagaaat ccagctaatt tggataaatt gacaggagct
     8101 ggtgcgattg ggcatgtgcg ttatgcgact gctggcgaag cttctgttga taacatccaa
     8161 cccttcctct tccgttttta cgatatgcag tttggcttgg ctcataatgg aaatctgacc
     8221 aatgcagcct ctctcaagaa agaactggaa caaagaggag caattttcag cgcgacttcg
     8281 gactctgaaa tcttggctca cctcattcgt cgcagtcata atcctagcct gatgggcaaa
     8341 atcaaggaag cgctcagcct tgtcaaaggt ggttttgcct atatcttgct gtttgaggac
     8401 aagttgattg cggctcttga cccaaatgga ttccgaccgc tttcgattgg taaaatggct
     8461 aatggagcag tagtcgtttc ctctgaaacc tgtgcttttg aggtcattgg tgccgagtgg
     8521 attcgtgatt tgaagccagg tgagattgtg atcattgatg acgagggcat tcagtatgat
     8581 agctatacag atgataccca gttggcgatt tgttctatgg agtatatcta ctttgctcgc
     8641 cctgattcta atatccacgg tgtcaatgtc catacggcac gtaagagaat gggagcacaa
     8701 ttggcgcgag aatttaagca tgaggcagat attgtagttg gtgtgcccaa ttcttcccta
     8761 agcgcggcta tgggatttgc ggaagaatca ggcttaccaa atgaaatggg tctgatcaaa
     8821 aaccaataca cccagcgaac ttttatccaa ccgactcaag aattgcggga gcaaggagtg
     8881 cggatgaaac tgtctgctgt ttcgggtgtt gtcaaaggca aacgtgtggt catggtggat
     8941 gattccattg tacgtggaac aacctctcgt cgtatcgttc agctcttgaa agaagcgggt
     9001 gcgactgagg ttcacgttgc cattggaagt cctgcactag cgtatccatg tttctacggg
     9061 attgatatcc agacccgtca ggagctgatt gcagccaatc atacggtcga agaaactcgc
     9121 caaatcattg gtgcggacag tctgacttat ctttcaattg atggcttgat tgagtcgatt
     9181 ggtatcgaaa cagatgcgcc gaacggtggt ctctgtgtcg cttactttga cggtgactac
     9241 ccaacgcctc tttatgacta cgaagaagac tatcgtagaa gtttggaaga aaagaccagt
     9301 ttttacaagt aggcgacaga ttctccatta aagaaaagga aaaaacaaat gacaaataaa
     9361 aatgcatatg cccagtcggg tgtggatgtt gaagcgggtt atgaagttgt tgaacggatt
     9421 aaaaagcacg tggcccgtac ggagcgtgca ggtgtcatgg gagctcttgg tggctttggt
     9481 ggtatgtttg acctttccaa gactggggtt aaagaacccg tcttgatttc agggactgac
     9541 ggtgtcggaa ccaagctcat gttggctatc aagtacgaca agcacgatac catcgggcag
     9601 gactgtgtgg ccatgtgtgt caacgacatc attgctgcag gtgcggaacc cctctatttt
     9661 ctcgactacg tagcgacagg gaagaatgaa ccagctaagc tagaacaagt ggttgctggt
     9721 gtggcagaag gttgtgtgca ggctggtgct gccctcatcg gtggggaaac ggctgaaatg
     9781 ccgggcatgt acggcgaaga cgactatgac ttggctggtt ttgcggtcgg tgtggctgaa
     9841 aaatctcaaa tcattgacgg ttcaaaggtg gtagagggag atgttcttct cggacttgct
     9901 tcaagtggga ttcactcaaa tggttactct ttggttcgtc gtgtctttgc ggattacaca
     9961 ggtgaggaag tcctaccaga attggaaggc aagaaactta aggaagttct acttgagccg
    10021 actcgtatct atgtcaaggc tgtcttgccg ctcatcaaag aagagttggt caacggcatt
    10081 gcccacatca caggtggtgg ctttatcgaa aatgtccctc gtatgtttgc agatgaccta
    10141 gctgctgaaa ttgatgaaag caaagttcca gtgcttccaa ttttcaaagc tcttgaaaaa
    10201 tatggtcaga ttaaacacga agaaatgttt gaaatcttca atatgggtgt gggacttatg
    10261 ttggcggtca gccctgaaaa tgtagagcgt gtaaaagaat tgttggatga agcagtctat
    10321 gaaattggtc gcatcgtcaa gaaagaaaac gaaagtgtca ttatcaaatg aaaaaaatag
    10381 cggtttttgc ctctggtaat ggctcaaatt ttcaggtgat tgccgaagaa ttttcagtgg
    10441 agtttgtctt ttcagaccat cgtgatgcct atgtgcttga gcgtgcaaag cagctcggcg
    10501 ttctgtccta tgcttttgaa ctcaaggagt ttgagagcaa gacagactac gaagcagccc
    10561 ttgtcgaact cttggaagaa caccagattg acttggtttg cctagcaggc tacatgaaaa
    10621 tcgttggacc aaccttattg tcggcttatg aaggtcggat tgtcaacatt catccagcct
    10681 acttgccaga atttccagga gctcatggga ttgaggatgc ttggaatgct ggcgtgggtc
    10741 agtctggtgt gaccattcac tgggtggatt cgggtgtgga tacaggccag gtcatcaaac
    10801 aggttcgtgt gccacgacta gctgatgata ccattgacag atttgaagct cgcatccatg
    10861 aagcagagta caggttgtat ccggaagtag tgaaggctct atttacagat tgactttttg
    10921 atgattcata tgatatcttt gattttaaat tggagtcagt gtttgttgaa gacggcttca
    10981 aacggaggta tttgtaatgt tagaatctaa aaaaacaact cgatatgtat tttatgtcta
    11041 tctgatgtta ttaacttggg gaatcttatt taagtttgaa acaaatcctg aatttatagc
    11101 atttttctta gctccaaggt atatcaattg gattccattt tcagaaccac taatagtcga
    11161 tggaaaaatt gtttttgctg aaatgttatt taatctgatt tcctttattc cattaggtgt
    11221 ttgtttccct ttgataaaaa ctaatttatc tagtttaaga atagtcggga caggtttctt
    11281 gattagttta ttgtttgagt gcttacagta tattttagca ataggtataa cagatataac
    11341 ggatttgact ttaaatacgc taggtgtctg tgtaggctta ctgatttatc aaatttttat
    11401 aagagtgttc aaatcacaga ctagaaaatg gatcaatatc ttaggtatgc ttagccttgg
    11461 ttttgcttat cttgttttac tgttactgca tttaattggt gtttaactaa tgattaaaaa
    11521 ggagaatata atgactaaac gcgtcttaat cagcgtctca gacaaagcgg gcattgttga
    11581 atttgcccaa gaactcaaaa aacttggttg ggagattatc tcaacaggtg gaactaaggt
    11641 tgcccttgat aatgctgggg tggataccat tgctatcgat gatgtgactg gtttcccaga
    11701 aatgatggac ggtcgtgtga agaccctcca cccaaatatc cacggagggc ttctcgctcg
    11761 tcgtgacttg gatagccact tggaagcggc taaggacaac aagattgagc tcattgacct
    11821 tgtggtggtc aacctttacc catttaagga aactatcctt aaaccagatg tgacttatgc
    11881 tgatgcagtt gaaaatatcg atattggtgg gccgtctatg cttcgttcag cagcgaaaaa
    11941 tcatgccagt gttacagttg tggtagatcc tgctgactac gctgtggttt tggacgaatt
    12001 ggcagcaaac ggcgaaacct cttatgaaac tcgccaacgt ttagcagcca aagtatttcg
    12061 tcacacagcg gcttatgacg ccttgattgc agaatacttc acagctcaag tgggtgaaag
    12121 caagcctgaa aaactcactt tgacttatga cctcaagcaa ccaatgcgtt acggtgagaa
    12181 tcctcaacaa gacgcggact tttaccagaa agctttgcct acagattact ctattgcttc
    12241 agccaaacag ctcaacggga aagaattgtc atttaataat atccgtgatg cagatgctgc
    12301 tatccgtatc atccgtgact tcaaagatag tccaaccgtt gtggctctca aacacatgaa
    12361 tccatgtgga attggtcaag ctgatgacat cgagactgct tgggactacg cttatgagtc
    12421 tgacccagta tctatctttg gtgggattgt cgtcctcaac cgtgaggtgg atgctgcgac
    12481 agctgagaag atgcacggcg ttttcctcga aatcatcatt gcaccaagct atacggatga
    12541 agcgctagcc attttgatca ataaaaagaa aaacttgcgt atccttgcct tgccatttaa
    12601 tgctcaagag gctagcgaag tggaagcaga atacacaggt gtagtcggtg gacttctcgt
    12661 gcaaaatcaa gacgtggtca aggaaagccc agctgactgg caagtggtga ccaaacgtca
    12721 gccaaccgag acagaagcga ctgctcttga gttcgcttgg aaggctatca agtacgtcaa
    12781 atcaaatggt attatcgtga ccaacgacca catgacactt ggtgttggtc caggtcaaac
    12841 caaccgtgtg gcttctgttc gccttgccat tgaccaagcc aaagatcgtc tggacggggc
    12901 ggtccttgct tcagatgcct tcttcccatt tgcggataac gtggaagaaa tcgccaaagc
    12961 aggaattaag gccatcatcc agcccggtgg ctctgtccgt gaccaagaat ccatcgaagc
    13021 agcggataaa tacggcttga ctatggtctt tacaggtgtg agacatttta gacattaaga
    13081 aaaa
//
LOCUS       DAVGJJ010000053        12922 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000053 DAVGJJ010000000
VERSION     DAVGJJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.053"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            16..738
                     /locus_tag="VVE35_001384"
                     /pseudo
     CDS             16..738
                     /locus_tag="VVE35_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            929..3220
                     /gene="pcrA"
                     /locus_tag="VVE35_001385"
     CDS             929..3220
                     /gene="pcrA"
                     /locus_tag="VVE35_001385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU3061564.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3263..3943
                     /gene="radC"
                     /locus_tag="VVE35_001386"
     CDS             3263..3943
                     /gene="radC"
                     /locus_tag="VVE35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU3061565.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEVCELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3940..4629)
                     /locus_tag="VVE35_001387"
     CDS             complement(3940..4629)
                     /locus_tag="VVE35_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU3061566.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4647..5288)
                     /locus_tag="VVE35_001388"
     CDS             complement(4647..5288)
                     /locus_tag="VVE35_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU3061567.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5443..5586)
                     /locus_tag="VVE35_001389"
     CDS             complement(5443..5586)
                     /locus_tag="VVE35_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU3061568.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRG"
     gene            complement(5594..5941)
                     /locus_tag="VVE35_001390"
     CDS             complement(5594..5941)
                     /locus_tag="VVE35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU3061569.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5946..7061)
                     /locus_tag="VVE35_001391"
     CDS             complement(5946..7061)
                     /locus_tag="VVE35_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3061570.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHPEESGIDFLTTSAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEELEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGIVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(7071..8030)
                     /locus_tag="VVE35_001392"
     CDS             complement(7071..8030)
                     /locus_tag="VVE35_001392"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU3061571.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(8143..8712)
                     /locus_tag="VVE35_001393"
     CDS             complement(8143..8712)
                     /locus_tag="VVE35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU3061572.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8840..9511
                     /locus_tag="VVE35_001394"
     CDS             8840..9511
                     /locus_tag="VVE35_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU3061573.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9495..10313
                     /locus_tag="VVE35_001395"
     CDS             9495..10313
                     /locus_tag="VVE35_001395"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU3061574.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10310..11206
                     /locus_tag="VVE35_001396"
     CDS             10310..11206
                     /locus_tag="VVE35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3061575.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     DIRLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLDDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11250..12224
                     /gene="pta"
                     /locus_tag="VVE35_001397"
     CDS             11250..12224
                     /gene="pta"
                     /locus_tag="VVE35_001397"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU3061576.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(12565..12864)
                     /locus_tag="VVE35_001398"
     CDS             complement(12565..12864)
                     /locus_tag="VVE35_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU3061577.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVIDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         3775 a         2480 c         2771 g         3896 t
ORIGIN      
        1 accacttttg aagagatgtt agctgtatta aaaacagctt atcaacttaa acacgcaaaa
       61 ggtggacgaa aacctaaatt aagcctagaa gaccttctta tgcccactct tcaatagtgc
      121 gagaatatcg aacttatgaa caaattgcgg ctgattttgg tattcacgaa agcaacttaa
      181 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggttttacg gtttcaagaa
      241 ctcctctcag ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc
      301 ctaaaaaaac aattagcgaa tgattctggt aaaaagaaat ttcacgctat gaaggctcaa
      361 gcgattgtca caagtcaagg gagaattgtt tctttggata tcgctgtgaa ctatagtcat
      421 gatatgaagt tgttcaaaat gagtcgtaga aatatcgaac aagctggtaa aatcttggct
      481 gacagtggtt atcaagggct catgaagata tatcctcaag cacaaactcc acgtaaatcc
      541 agcaaactca agccgctaac agctgaagat aaagcctata accatgcgct atctaaggaa
      601 agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatatt ttcaacaacc
      661 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat
      721 catgaactag gattctagtt ttgcaggaag tctattgagg tattgagcta gtttatgaaa
      781 aaattgggtg aaaagtcgag tgttttagaa acccacagtg tagtattcta gtttcaatcc
      841 actatatttt gctactcccc gtaaagtttc tattttccct gatttctgat ataatagaaa
      901 tattgacttc aagagtaagg aagagaagat gaacgcatta ttaaatggaa tgaatgaccg
      961 tcaggctgag gcggtgcaaa cgacagaagg tcccttgcta atcatggcag gggctggttc
     1021 tggaaagact cgtgttttga cccaccgtat cgcttatttg attgatgaaa agctggtcaa
     1081 tccttggaat atcttggcca ttacctttac caacaaggct gcgcgtgaga tgaaagagcg
     1141 tgcttatagc ctcaatccag cgactcagga ctgtctgatt gcgaccttcc actccatgtg
     1201 tgtgcgtatt ttgcgtcgcg atgcggacca tattggctac aatcgtaatt ttacaattgt
     1261 ggatcctggt gaacagcgaa cgctcatgaa acgtattctc aaacagttga acttggatcc
     1321 taaaaaatgg aatgaacgaa ctattttggg gaccatttcc aatgctaaga atgatttgat
     1381 tgatgatgtt gcttatgctg cccaagctgg cgatatgtat acgcaaattg tggcccagtg
     1441 ttatacagcc tatcaaaaag aacttcgtca gtctgaatcc gttgactttg atgatttgat
     1501 tatgctgacc ttgcgtctct ttgatcaaaa tcctgatgtt ttgacctact accagcaaaa
     1561 attccaatac atccacgttg atgagtacca agataccaac cacgctcagt accaattggt
     1621 caaactcttg gcttcccgtt ttaaaaatat ctgtgtggtt ggggatgcgg accagtctat
     1681 ctacggttgg cgtggtgctg atatgcagaa tatcttggac tttgaaaagg attaccccaa
     1741 agccaaggtt gttttgttgg aggaaaatta ccgctcaacc aaaaccattc tccaagcggc
     1801 caacgaggtt attaaaaata ataaaaatcg ccgtcctaaa aatctctgga ctcaaaacgc
     1861 tgatggggag caaatcgttt actatcgtgc cgatgatgag ctggatgagg ctgtatttgt
     1921 agccagaacc atcgatgaac ttagtcgcag tcaaaacttc cttcataagg attttgcagt
     1981 tctctatcgg actaatgccc agtcccgtac aattgaggaa gccctgctca agtctaacat
     2041 tccttatacc atggttggcg gaaccaaatt ctacagccgt aaggaaattc gcgatattat
     2101 tgcttatctc aaccttattg ctaatttgag tgacaatatt agttttgagc gtattatcaa
     2161 cgagcctaaa cgtggaattg gtctaggtac agttgagaaa atccgtgatt ttgcaaattt
     2221 gcaaaatatg tctatgctgg atgcttctgc taatattatg ttgtctggta tcaagggtaa
     2281 ggcagcccaa tctatctggg attttgccaa tatgatgctt gatttgcggg agcagctaga
     2341 ccacttaagc attacagagt tggttgagtc cgtcctagaa aaaacaggtt atgtcgatat
     2401 tcttaacgcc caagcgactc tagaaagcaa ggcacgggtt gaaaatatcg aagagtttct
     2461 ttctgttacg aagaactttg atgacaccac ggatgtgaca gaagaggaaa ctggtctgga
     2521 caaactgagt cgtttcttaa atgacttggc tttgattgcc gacacagatt caggtagtca
     2581 ggagacatca gaagtgacct tgatgaccct gcatgctgcc aaaggtctcg aatttccagt
     2641 tgtctttttg attgggatgg aagaaaatgt ctttccactt agtcgtgcga ctgaagatcc
     2701 agatgaatta gaagaagagc gccgtctagc ctatgtaggt atcacgcgtg cagagaaaat
     2761 tctctatctg accaatgcca actcacgctt gctttttggt cgtaccaatt ataaccgtcc
     2821 gactcgtttt attaacgaaa tcagttcaga cttgcttgag tatcaaggtc tggctcgtcc
     2881 tgcaaataca agctttaagg catcatatag cagtggtagt atttcctttg gtcaaggtat
     2941 gagtttggct caggctcttc aagaccgtaa acgcggtgct gccccaaaat caatccagtc
     3001 aagcggtctt ccatttggtc aatttacagc tggcgcaaaa ccagcatcta gcgaggcaaa
     3061 ttggtccatt ggtgatattg ctctccacaa gaaatgggga gagggaaccg ttctggaagt
     3121 ttcaggtagc ggtgctaggc aggaattgaa aatcaatttc ccagaagtag gtttgaaaaa
     3181 acttttagcc agtgtggctc caattgagaa aaaaatctaa ttttccatcc ttctcacgaa
     3241 taataaagtg aggaggattt ttatgtacag tatttcattc caagaagatt cactattacc
     3301 aagagaaagg ctggccaagg aaggagttga agcgcttagt aaccaagagt tgctagctat
     3361 tttactcagg acaggaacac gtcaagctag cgtttttgaa attgcccaaa aagtcttgaa
     3421 caatctttca agcctaacgg atttgaaaaa aatgaccctg caggaattgc agagtttgtc
     3481 tggtattggg cgtgttaagg ccatagaatt acaagctatg attgaactgg ggcatcgtat
     3541 tcacaaacac gagactcttg aaatggaaag tattctcagc agtcaaaagt tggccaagaa
     3601 gatgcagcag gaattagggg ataaaaaaca agagcacctg gtggcactct atctcaatac
     3661 tcaaaatcaa atcatccatc agcagaccat ttttatcggg tctgtaactc gtagtatcgc
     3721 tgaaccgcga gagattcttc actatgcaat caagcatatg gcgacttctc ttatcttggt
     3781 ccacaatcat ccttcaggag cggtagcgcc tagccaaaat gatgatcatg tcactaaact
     3841 tgttaaagaa gtctgcgaac tgatggggat tgttctcttg gaccatttga ttgtctctca
     3901 ttctaattac tttagttatc gtgaaaagac agatttaatc taaagttcat taacgacata
     3961 gtcaaagagt tttttatctt tgggacgatt ttcaaaaaga agttctggat gccattggac
     4021 accgagaaag gcgacatcat ccgtactcat gacagcctca atgataccat ctttaggatc
     4081 atgagccaca acttttaaat ttggtgctaa gtccttgatg ctctggtggt ggaaggagtt
     4141 gatatgagag atttctccat agatttcttg gagaacggta tctggttctg tcaccaagcg
     4201 ttgagttgtg tactcaacag aagaatcctg ccaatggtct tcgatatctt ggtacaaagt
     4261 tccacccatg gcaacgttaa agagttgggt accacggcag acagagaaaa tgggcttttt
     4321 ctgtttaata gcttccttga tgagggccag ttcgaagata tctctttgaa ggtgatagtc
     4381 atcgctatca atggttttgg gttcgccata gaattttgga tcgacatttt gcccacctgt
     4441 caagatgagc ttgtcaatca aactgatata gtggcaggcc atttcttgat caccaatcgg
     4501 taggatgatg ggaatccctc cagcatcttt aacgccttca acaaagcctt ttgctgcgta
     4561 gctcatcatg atgtcatcat ctggatgagc tttttcgttt cctgtaatcc caataactgg
     4621 ttttttcata aaatgatttt cgctttctaa tcctcttttc gcatgaagta gaggagggtt
     4681 tggagttcac ttgtcaaatc gacatactga acgaccacgt cttttggtaa atgcagatgg
     4741 actggtgaaa aactgagaat tcctttcaca ccagcatcaa ccaagagatt agcaacctct
     4801 tgtgacttga cgctgggaac agttaggata gcagtcttca catcagcatc cttgatttta
     4861 tccttgatct gagaaatccc gtaaatggga atcccgtcag gagtttgggt accgacttca
     4921 ggatggtcgt ctaggtcaaa ggccatgata atcttcatct tgttacgttc gtggaagcgg
     4981 tagtggagaa gggcatggcc catatttcca ataccaacca gcatgacatt ggtaatagag
     5041 ttgtcattga gcaaatcggc aaaaaatgtc attagttttt tgacatcata gccaaaacca
     5101 cgacgaccaa gttcaccaaa ataggaaaaa tcacgacgta cggtcgctga atcaataccg
     5161 atagcctctg caatttgctt agagttggca cgttcaatct tttctgcatg aaatctctta
     5221 aaaattcgat agtagagaga gagtcttttt gctgtagctt ttggaatagc aaactgttta
     5281 tctttcacaa aatcacaacc tttctattct tctattttat agaaacattg tgaaaaaatc
     5341 aacaaaaata agaaaaaact aagaaaaatc ttagttttga tgtaaaaaat ctgcatgaga
     5401 tagaaaacgg tagaggtctc cgaccagacc ctgataaact ttttagcccc taaaagtcag
     5461 agaagtcaca taaagtgtat ctggtaaggt tacacatcct gacaaagtca acatgagagc
     5521 ctcatgatcc tcatacttga gagtacgctc tacatgatag cagtccttat aggtcagttc
     5581 aaacattttg gctctatctt tccgattttg taaagacacc acgttctacc aagctatcca
     5641 tgaggaagta gaatttttcc tgatgaatat ggtggtcttc tgatttgaaa atatcaacta
     5701 gacgaaggcc aaacttgtca gtgatattga ttttagcccc tgtaagttcc ttgttaatga
     5761 tgattttgag ttggaagcct tcaccgctgt ttggcacttt ttccaaaagg cgagtcagtt
     5821 catagttacc aaccttagtt tcaaaaaagg tgttatcttt gagggtgaat tttttaacag
     5881 aaggactaag agtgtaatcg taacgacaat tttttaactg aatgattttt tcaaatgcca
     5941 tatggctaac ctccgataat ttcttttaag gtttttgcga gggtttgtag gtcttcaacg
     6001 gtattttgtg gcgacaaact gatgcgaagg gattccttca agcgttctga atttgcgcca
     6061 tacatggctt caagaacatg gctggattgg acaatgcctg cagtacaggc tgagccagta
     6121 gagattgaaa ttccagctaa atctagccga aggagtaaga ggtcattttt ctgaccagga
     6181 aatccaatat tgagaacata agggagatga tgttttcctc tattcaagta atactgaatg
     6241 ccctccagct ctgccagaaa ggcagtttct agattttgta catgttgaaa atgttcttct
     6301 tgtttttcta gttcttcttt taaggctgca accatgccta caatggcagg cagattttca
     6361 gttcctgcac gttttttctg ttcctggtct ccgccatgta gataggaatc aaagtccatg
     6421 ctagatgcgt agagaaaacc gattccctta ggaccatgga atttgtgggc agaagtagtg
     6481 agaaaatcaa tgcccgattc ttctggatga atagggattt taccaatagc ctgaactgca
     6541 tcgacatgat aggcggctgg gtgttgcttg agtatttggc caatttcagc gatgggcagt
     6601 aggtttcctg tctcattatt ggcaaacatg gtagaaacca aaatcgtatc gtcacgtaaa
     6661 gccttttgaa tttgctgggc tgtgatttct tgattttctg gctggataat ggttgcttca
     6721 aacccaaagt gttgaaccaa gtaatcaatt gtttcaagga cagcatggtg ctcgatggca
     6781 gttgtgatga tatgttttcc ttgttcttgg tgacgaagac agtagccaat gatggtagta
     6841 ttattgcctt cagtcccacc agaagtgaaa aagatatgtt gaggttttgt ccttagtaac
     6901 tgggctagtt cctgacgggc ttctcgcaag agtttgccag cttgacgacc atgaccatga
     6961 atactagaag gatttccgtg ggtttcttgc ataaccttgg tcatagctga aatagcaact
     7021 gctgacatag gagtcgttgc agcattgtcc aaataaatca aagaatcacc ttatttcttt
     7081 ttattgtagg caaagagtgg gctgactggt tttctttcgt gaatacggac gatagcatca
     7141 ccaattaact cactagcagt gatgtagcat acatttttag gagttttttc ttttgttgct
     7201 actgaatcag tcacaagaat ttctttaata ttagtattgt caagaagctc agcagctccc
     7261 tcgacgaaga gaccgtggct agaaacagca taaatttctg tagctccttc acgttcaacg
     7321 attttagaag cttcagagaa ggtacgtcct gtatttaaaa tatcatcaat caagatagct
     7381 ttcttacctt caacatcacc aataatataa ccttcgttac gagttgcatc gtcttgaggg
     7441 tagtcgataa tggcgatagg agcatcaaga tattcagcca ggctacgcgc acgtttgaca
     7501 cctgaatttt tagggctaac gacaacaaca tctgaaccaa gcaatccttt atcgcagtaa
     7561 tgttttgcga ataggggaac agtgaaaaga ttatccactg gaatatcaaa aaaaccttga
     7621 acctgaacgg catgcaaatc aagagtcagg atacgatcaa ctccagcctt aaccagcata
     7681 ttggcaacta gttttgctgt aagtggctca cgaggacaag caatgcggtc ttgacgtgca
     7741 tagccaaaat atggaaggac aacgttgata ctgtgggcac ttgcacgcac acaagcatcg
     7801 accatgatta acagttccat taggtggttg ttgacaggga aacttgttga ttggatgatg
     7861 taaacatcat aaccacggac actttcttcg atatttactt ggatttctcc gtctgaaaat
     7921 tgacgtgatg atagttttcc aagtgggaca ccaacagctt gggcaatttt ctgtgcaatc
     7981 tcttggttag agttaagtgc gaaaagtttc atgttttttc tatctgacat tatagaccgt
     8041 cctctgtaaa ctttataaat cctagttata tttaccttac atatatgaac tagggtttgt
     8101 gtatttttat cttttctatt ttaccaaaaa atggagatta tttcagctat ttttcatact
     8161 tttgacaaat cgaaccaatt ttgaaggagc tttttggtag gaaatctgat ttttctctaa
     8221 aaattgtcgg aaatcctgtt tgccttgctc atgattttcc acttcaagct ccaattcgta
     8281 atctgtcata tcaaagtatc ggctctgatc cagtgccatg agaccaatag ctgttttcat
     8341 ttcatagcga agcgttgtta gacaaccaag aacctgccag ttcttacttt ggataccatg
     8401 tttcgccaat tcatccagta ctagtccttg aggaagttct tccttactca gatagttctc
     8461 agcatctttt agttgcaatt tttggttgta ttccatgttt ccaacactct gcgggacttt
     8521 gagtgtcaac tcagcccagt cttcaaaggt tcgaatgcgc atagcgactt tcttttctcg
     8581 cagttcaaaa tcaggcgtgt ctatgtagta atttgtttga agaacaggag tgacacctgt
     8641 gaactggtct tttagacgat tgtattcatc ttttttcaat agtgttttca attcaatttc
     8701 taaatgtttc atttttctta ccttttttta tcgttgaaag cggatttatg gtataataag
     8761 cattgtattt attgtatatg aatctggaga aaaaatcaaa gatatttttg acggataata
     8821 tgagaacaag ggagaatata tgaccttaga atgggaagaa tttctagatc cttacattca
     8881 agctgttggt gagttaaaga ttaaacttcg tggtattcgt aagcaatatc gtaagcaaaa
     8941 taagcattct ccaattgagt ttgtgaccgg tcgagtcaag ccaattgaga gcatcaaaga
     9001 aaaaatggct cgtcgtggca ttacttatgc gaccttggaa cacgatttgc aggatattgc
     9061 tggcttacgt gtgatggttc agtttgtaga tgacgtcaag gaagtagtgg atattttgca
     9121 caagcgtcag gatatgcgaa tcatacagga gcgagattac attactcata gaaaagcatc
     9181 aggctatcgt tcctatcatg tggtagtaga atatacggtt gataccatca atggagctaa
     9241 gactattttg gcagaaattc aaattcgtac tttggccatg aatttctggg caacgataga
     9301 acattctctc aactacaagt accaagggga tttcccagat gagattaaga agcgactgga
     9361 aattacagct agaatcgccc atcagttgga tgaagaaatg ggtgaaattc gtgatgatat
     9421 ccaagaagcc caggcacttt ttgatccttt gagtagaaaa ttaaatgacg gtgtaggaaa
     9481 cagtgacgat acagatgaag aatacaggta aacgaattga tctgatagcc aatagaaaac
     9541 cgcagagtca aagggttttg tatgaattgc gagatcgttt gaagagaaat cagtttatac
     9601 tcaatgatac caatccggat attgtcattt ccattggcgg ggatggtatg ctcttgtcgg
     9661 cctttcataa gtacgaaaat cagcttgaca aggtccgctt tatcggtctt catactggac
     9721 atttgggctt ctatacagat tatcgtgatt ttgagttgga caagctagtg actaatttgc
     9781 agctagatac tggggcaagg gtttcttacc ctgttctgaa tgtgaaggtc tttcttgaaa
     9841 atggtgaagt taagattttc agagcactca acgaagccag catccgcagg tctgatcgaa
     9901 ccatggtggc agatattgta ataaatggtg ttccctttga acgttttcgt ggagacgggc
     9961 taacagtttc gacaccgact ggtagtactg cctataacaa gtctcttggc ggtgctgttt
    10021 tacaccctac cattgaagct ttgcaattaa cggaaattgc cagccttaat aatcgtgttt
    10081 atcgaacact gggctcttcc attattgtgc ctaagaagga taagattgaa cttattccaa
    10141 caagaaacga ttatcatact atttcggttg acaatagcgt ttattctttc cgtaatattg
    10201 agcgtattga gtatcaaatc gaccatcata agattcactt tgtcgcgact cctagccata
    10261 ccagtttctg gaaccgtgtt aaggatgcct ttatcggtga ggtggatgaa tgaggtttga
    10321 atttatcgca gatgaacatg tcaaggttaa gaccttttta aaaaagcacg aggtttctaa
    10381 gggattgctg gccaagatta agtttcgagg tggagctatt ctggtcaata atcaaccgca
    10441 aaatgcaacg tatctattgg acgttggaga ctacgttacc attgacattc ccgctgagaa
    10501 aggctttgaa accttggagg ctattgagct tccattagat attctctatg aggatgacca
    10561 ctttctagtc ttgaataaac cctatggagt ggcttctatt cctagtgtca atcactctaa
    10621 taccattgcc aattttatca agggttacta tgtcaagcaa aattatgaaa atcagcaggt
    10681 tcacattgtt accagactag atagggacac ttctggcttg atgctctttg ccaagcacgg
    10741 ttatgcccat gcacgattag acaagcagtt gcagaagaaa tctatcgaga aacgctactt
    10801 tgctttggtt aagggagatg gacatttgga gccagaaggg gaaattattg ctccgattgc
    10861 gcgtgatgaa gactccatta ttaccagacg ggtggctaaa ggtggaaaat atgcccatac
    10921 ttcctacaag attgtagctt cttatggaaa tatccacttg gtcgacattc ggctgcacac
    10981 tggtcgaacc caccaaattc gagtccactt ttctcatatt ggttttcctt tgttgggcga
    11041 tgatttgtac ggtggtagtc tggacgacgg catccaacgt caggccctgc attgccatta
    11101 cctatccttt tatcatccat ttttagagca agatttgcag ttagaaagtc ccttgccgga
    11161 tgatttcagc aaccttatta cccagttatc aactaatact ctataaaaac tgtttcagag
    11221 tataattatt atcttaaagg agaaaactca tggaagtttt tgaaagtctc aaagccaacc
    11281 ttgttggtaa aaatgctcgt atcgttctcc ctgaagggga agagccacgt attcttcaag
    11341 cgactaaacg tttggtaaaa gaaacagaag tgattcctgt tttgcttgga aatcctgaaa
    11401 aaattaaaat ttatcttgaa attgaaggaa tcatggatgg ttatgaggtc atcgaccctc
    11461 aacattatcc tcaatttgaa gaaatggttt ctgccttggt ggagcgtcgc aagggcaaaa
    11521 tgactgaaga agatgtacgc aaggttttgg ttgaagatgt caactacttt ggtgtgatgt
    11581 tggtttactt gggcttggtt gatggaatgg tgtcaggagc gattcactca acagcttcaa
    11641 cagttcgtcc agctctacaa atcatcaaaa ctcgtccaaa tgtaactcgt acttcaggag
    11701 ccttcctcat ggttcgtggt acggaacgtt acctatttgg agactgtgcc attaacatca
    11761 atccagatgc agaagccttg gctgaaattg ccatcaactc agcaatcaca gctaagatgt
    11821 ttggcatcga acctaaaatt gccatgttga gctattctac taaaggttca gggtttggtg
    11881 aaagcgttga taaggtcgtt gaagcaacta aaattgctca cgacttgcgt cctgaccttg
    11941 aaatcgatgg tgagttgcaa tttgatgcag cctttgttcc tgaaactgca gctctgaaag
    12001 ctcctggaag tacggtagct ggtcaagcaa atgtcttcat cttcccaggt atcgaggcag
    12061 gaaatatcgg ttacaagatg gctgaacgcc tgggtggctt tgcggctgta ggacctgttt
    12121 tgcaaggttt aaacaagcca gttaatgatc tttctcgtgg atgtaatgca gatgatgttt
    12181 acaagttgac cctcatcaca gcagctcaag cagttcatca atagtgaaaa ctataaagcg
    12241 atatactatg ctatactgta gttatgaaac tatgtacgaa aagcactgcc attaattcct
    12301 gagaactaaa ttactgattg gtgtcaaaaa ggaaaacttc caagcgatga tatcctgtct
    12361 atacacgacc tatagaaatc tgtaatatgt atgtccgtaa aacgataatt tccctttttg
    12421 attttaaatg agtatgaaaa gagaattttt catgagaaaa cgcataatat taagtgtctt
    12481 gacacctgct attatgcgtt ttttataatt tttgcacctt tcttagcctt tgttatcgac
    12541 aagtattgtt ctagctcctt gtttttagaa tggcaatttc ccagcgaaag cacccagttt
    12601 tttcttagta gtttcatcga tttgttcaag agcagagttg atggcttgaa cggtcatatc
    12661 agaaagagtt tcaaggtcct ctgggtcaat gacagctgga ttgaaatcaa tgctgacaac
    12721 tttcttatct ccagttaagg tcgcttggac aagatcttga gcagatttgc caacaaattg
    12781 catagcagca agttcagctt ggctttgttc catttgtttt tgaagttttt gtgcttgacg
    12841 catcatgttt tgcatgttca tcataagatt tctaaagttc ctttcatctc aaggggataa
    12901 tccttgttgt taacaagtgc ct
//
LOCUS       DAVGJJ010000054        12752 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000054 DAVGJJ010000000
VERSION     DAVGJJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.054"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..242
                     /locus_tag="VVE35_001399"
                     /pseudo
     CDS             1..242
                     /locus_tag="VVE35_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(359..706)
                     /locus_tag="VVE35_001400"
     CDS             complement(359..706)
                     /locus_tag="VVE35_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3061578.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(817..2592)
                     /gene="spxB"
                     /locus_tag="VVE35_001401"
     CDS             complement(817..2592)
                     /gene="spxB"
                     /locus_tag="VVE35_001401"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU3061579.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2799..5042)
                     /locus_tag="VVE35_001402"
     CDS             complement(2799..5042)
                     /locus_tag="VVE35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU3061580.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QAAGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5052..5423)
                     /locus_tag="VVE35_001403"
     CDS             complement(5052..5423)
                     /locus_tag="VVE35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU3061581.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5434..5829)
                     /locus_tag="VVE35_001404"
     CDS             complement(5434..5829)
                     /locus_tag="VVE35_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU3061582.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6013..6111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6195..6986
                     /gene="thiD"
                     /locus_tag="VVE35_001405"
     CDS             6195..6986
                     /gene="thiD"
                     /locus_tag="VVE35_001405"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU3061583.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIHPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7196..7828)
                     /locus_tag="VVE35_001406"
     CDS             complement(7196..7828)
                     /locus_tag="VVE35_001406"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU3061584.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7821..8624)
                     /locus_tag="VVE35_001407"
     CDS             complement(7821..8624)
                     /locus_tag="VVE35_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU3061585.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8624..9148)
                     /gene="thiW"
                     /locus_tag="VVE35_001408"
     CDS             complement(8624..9148)
                     /gene="thiW"
                     /locus_tag="VVE35_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU3061586.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9153..9845)
                     /gene="tenA"
                     /locus_tag="VVE35_001409"
     CDS             complement(9153..9845)
                     /gene="tenA"
                     /locus_tag="VVE35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU3061587.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQVMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9856..10506)
                     /locus_tag="VVE35_001410"
     CDS             complement(9856..10506)
                     /locus_tag="VVE35_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU3061588.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10508..11893)
                     /locus_tag="VVE35_001411"
     CDS             complement(10508..11893)
                     /locus_tag="VVE35_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061589.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVNQISIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAILFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMIQVTSKKVASSPELFHMNRVIGELGNRPLFSIANFTFYQGISCILGDNGVGKSTF
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDSTDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11894..12454)
                     /locus_tag="VVE35_001412"
     CDS             complement(11894..12454)
                     /locus_tag="VVE35_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3061590.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3844 a         2827 c         2440 g         3641 t
ORIGIN      
        1 gacaatgcta tatggcataa atcaagtgcc ttaaagattc cgactaatat tggctttgca
       61 tttattcctc catacacacc agagatgaac ccattgaaca agtgttgaaa gagattcgta
      121 aacgtggatt taagaataaa gcctttcgaa ctttggaaga tgtcatacaa ggactggaga
      181 aggagatgat aaagtccatc gttaatcgga gatggactag aatgctttgt gaagacagat
      241 gagtattata tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt
      301 catattctca atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct
      361 atttcatacg ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg
      421 ttccccaatc ggttacagtt ggtccgtgta aaacctttat accaagctcg ttcaaccgtt
      481 tgtagttctg gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt
      541 ccaaaggaac caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag
      601 caaaaccatc atcagcataa tctgcctttt tatccaaaat atgctccaag tcagcacaga
      661 cttggggaac atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat
      721 aaaaagaccg gattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag
      781 tctacaagtt tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa
      841 tccttcttct tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt
      901 gaaggctttg atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg
      961 aagtggacgg tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa
     1021 tttaacagct tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac
     1081 agctccttga gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa
     1141 gtgtttgttt gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac
     1201 aaggttgata actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt
     1261 gaatgctccg tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc
     1321 gataccacca ggaagggcaa tacccattgt cgcaaagagt ggagatgtac gccacatgtt
     1381 cttaggtgtc atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga
     1441 gtagatagcg tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa
     1501 ttcaccctca gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt
     1561 aacgtttgca cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt
     1621 agctgcttga ccagcatcac caaggattga agcgtcaagg gcatgacgtt taccaagttt
     1681 gtaagggtcg atatcgactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc
     1741 agcaaatggg aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt
     1801 ggctggtttc caaccaacac ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa
     1861 agcttcaaag tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt
     1921 aatcacttca ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt
     1981 gttcaagatt tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga
     2041 gcgttcgtat gaacctgaac cgtagtatga gttttcatcg atttcttgga aaccgaagtt
     2101 tactggaatt tcaacaacag ctggaccttt tttagaaatt gcagcacggc aggcttcgtc
     2161 aattactttt ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt
     2221 gtacattggg ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg
     2281 tgatccaagg atcgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat
     2341 caagtgagtc gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt
     2401 agcttgcata accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaagcggat
     2461 atctttgtct tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta
     2521 gattgtatct acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt
     2581 cccttgagtc ataatgataa ctctccttca atttttttaa acttggagaa tacgattaca
     2641 tagaattgga aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt
     2701 ttctaaaaat ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagtgttgt
     2761 catttctgtt ttataacaaa gaaatctagt cattactttt agtctatttt actaaaattt
     2821 aacagaaggg aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat
     2881 tctgggttga gagccagtcc aacacctgaa aagactcctg ctgcaatcgg aattccgaca
     2941 acattgtaga taaaagccca gaaaagattg agtagaattc gatgaaaggt tttcttactc
     3001 atatcaaagg cacgaaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac
     3061 tcgatggcga tatctgttcc agcacccata gcaattccca catctgctac actaagggca
     3121 ggagcgtcat tgataccgtc cccaacaaag gctactttgc cagccgcttg cagtttatgg
     3181 atttcatggg ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga
     3241 tctgcaatag cacgcgccac accagcattg tctcctgtca gcatgactgt tcggagacca
     3301 cgttttttta gctgactgat ggctagctta gcattttcct taggaatatc ttgcaaagca
     3361 agcaagcctt tgatttcatt gtcaacagct aaaaacacaa ctgtcttagc ttctttttct
     3421 agttcttcta gtttatcttg ataagtatta gaaatatcca tgccatccag cattttagca
     3481 tttccaagta aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggcc
     3541 tgaaaatttt caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc
     3601 tcagccagtg ggtgttgaga agcatcttcc aaggaggctg ccaatccaaa cacttctact
     3661 tcgtcgccga tgatatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca
     3721 aagacaagag tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc
     3781 cccatcttgg cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag
     3841 gcacaaggac aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta
     3901 gctccaagca cgaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt
     3961 cctaaaatga caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc
     4021 ggcgcacgac ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct
     4081 gagccaactt tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg
     4141 acagtatctc caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca
     4201 atactagaga caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact
     4261 cgaatcaggt cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc
     4321 aagacttctg cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt
     4381 tttctcattt tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca
     4441 ctttcgaagt aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact
     4501 agagttccca gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa
     4561 gcactctgga tatatggctt acctgcaact aacataatag gcgttgttgc tagaaaggtt
     4621 ccccaatgca tgacttgatg actaatgcta cctgtcaaca tcccaatcat gagaatcaca
     4681 agaggcacag taaagatact agtaatccaa aaacgttgca ggagagatag agattttcga
     4741 gtcttctcaa cgactatata gcttcccttt tgcatcttca tgccacaaga aaattcatgt
     4801 cgccctaatt cttgaggtgt aaaacgaatg actttctcct catctacgcc gattggttcc
     4861 aagatacctt cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga
     4921 aaggtaatct cagctggaat tcccttttga agctggatat gggctggatg atagcctttt
     4981 tcagctcgga tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc
     5041 atagtctcca cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag
     5101 ccaaactctc cagcctgctc aggcgtgatt tccactacat actcttcccc cattggcagg
     5161 ttcgtatgta caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatccttg
     5221 cggtcaaaga caatgcgtgc tggcactgat ttcttgagga caatcaactc aggagtatag
     5281 cctcccatga cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca
     5341 gatttttcag gctttttgaa aaaccaaaac aagataaacg cgataagggc aatacaaata
     5401 atggttacaa tactatttaa catgacgtct cctttacata caattacatc ttacttctgt
     5461 tacagcactt gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt
     5521 aaattcacat tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt
     5581 gtctttgata tcttggacaa gtaaatcccg actttggtct agagttaaaa gggctgaata
     5641 aacaaaggac ttgccttctt tttttcgagt caaacactct ttctcaacca gccgagccaa
     5701 aagtgtctga accgtggact tggaccaatc aaaccgctct gccaaaaccc taatcaaatc
     5761 cgtactggtc tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga
     5821 aatctgcatt accatacctc caaaatctac atttgtcaat tacactcatc agtatactct
     5881 taaaatctac atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc
     5941 atttgaaaaa cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta
     6001 aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc
     6061 ctctgactca atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca
     6121 gtcttctatt gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg
     6181 ttgggaggtt ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta
     6241 gtggtggtgc tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa
     6301 tggctgttgt aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg
     6361 tttctcctca aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg
     6421 ctgtaaaaac tggaatgttg gctactactg aaatcatgga aatcatccac ccctatctta
     6481 aaaaactgga ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct
     6541 tgattgactc aagtgctaga gactatctca aaacaaactt actacctcta gcaactatta
     6601 ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag
     6661 acatgcagcg tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca
     6721 aaggcggaca tctcaaaggt ggtgctaaag atttcctctt taccaagaat gaacaatttg
     6781 tctgggaaag cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg
     6841 cagtgattac tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca
     6901 aggcctttat cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag
     6961 tcaaccatac aacttttaaa gattaagaaa aaaaactctc tagttcccac tttaagggaa
     7021 ttagagagtt tttatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcaac
     7081 ctcaaaacac tgttttgagc tgacttcgtc agtcttatct aaaacttcaa ggcagtactt
     7141 tgagcaacct gcgactagct ttctagttta ctctttgatt ttcattgagt attaattagg
     7201 aaagaatgtc atgcaacttt gttaaaaagg cttgcgtttt tgcctcaata tcttctgctt
     7261 gcatcaaatc acgtacaaca gctacaccag ctatgccagt gcccataagc tgatcaatat
     7321 tctccgaagt caagcctcca atagcaacta ctggaatggc aaccgtttgg caaattgttt
     7381 tcaaggtcga tatcagagta atgggcgcat tttccttggt ggtggttggg aaaatggctc
     7441 ctgtacccaa gtaatctgca cctgatttct ccgcttccag agctctttta accgttttag
     7501 cggtgacacc gaggattttt tcaggaccca agactttgcg agctaccgaa actggtaatt
     7561 catcatctcc gatatgcaga cctgctgcat caaccgcaag acaaacatcc aaccgatcat
     7621 cgattatcaa gggtacctga taagcatctg ttatttcctt gacttgtttt gccagttgat
     7681 aatattgatt ggttgtgaga tttttttctc gcaattggac tatggtaacc cctgaacggc
     7741 aggccgtctc aacttttgca agaaagcttt ccacggaatc ttgatagcga ttggttacca
     7801 gatatagtct aagtgcttct ctattcataa acctctcctt tgatggtatc tagccaattt
     7861 tcatctcttc ttaggagcga aagctgattg agtacttggt aacgaaattc ttccaatccc
     7921 attccttgaa caactatttt ctcagcagcg atattgagat aagagactgc taagcaagaa
     7981 gcttcaaaac cagtctttcc ttggctgaga aaaacggctg ttaaggctcc aaccaagtct
     8041 cctgtccctg ttatccagtc taattcagta cagccattct ccagtacagc aacttgattc
     8101 tccgaaacaa taaggtcctt ggggccagta actaagaatg acataccagg ataggtctga
     8161 caccagtctt tcaagacttg aagcaaatcc tccgtttctt gatctttagc actcgcatcg
     8221 accccaacgc cgtggtgctt taatccaaca agacttcgaa tttctgacat gtttccttta
     8281 aggaccgtag gtctatagtc taaaaggtct ctgactaagc tcttacgaat ggatgaagct
     8341 gttacgccaa ccgcatctac taccattggg agacaaactt gagctgcata agaagctgct
     8401 atacggattg ctttctcctt ctcagctgac aaatgcccca aattgatgaa gagagcctga
     8461 ctttgcttag taaaatcaag aacctcacgg ggatcatctg ccatgacagg tttgcatccc
     8521 agagccaaaa tcccatttgc cagcatctca caagaaatct cattggtcat acagtgaatg
     8581 agggaactag agcctatagg aaagggattt gtaaattcct gcatcagtct atcctttcac
     8641 taaagaaata tccctgcact tttttaaaga attcctgctt gattaaaaat cgaaaggcaa
     8701 taaaggaaat cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc
     8761 agctaagctt agcagctgat cctgtaaaga gtaccataac aggataggaa acaatggaac
     8821 caataatacc tgttcccaaa atctctccta gagcagaata gtgaaatttt cgaccgtact
     8881 tataaaagag acctgccaga agggctccaa aagtcgctcc tgtgagagct aaaggcggaa
     8941 tcccttgagt cgtcatacgg ataaaggctg tgactgtagc catagccaag gcataaacag
     9001 gtcccatcat gattcccgct agaatattga ctacactgga catcggtgcc attccctcaa
     9061 ttcgaaagat aggtgtaagg actacatcaa gggcaatcat catagataaa atggttaatt
     9121 tgtgaacttg taattggtgc tttctcatgc ttctattctt ctcctttttc caaagactgt
     9181 aaatcgctct tccatgtctg gtgttggtag gccatttccc aaaacttggc ttccatatga
     9241 acactgatgt ggaaggcatc tagcattttt tgcttgtctg tctcgtcact ttctcgatag
     9301 agctgattga ccagtgctcc ctcctctctg atctgttgct ctaactcatc cgtaatataa
     9361 gtttcaatcc attgttgata gagaggattt ggtgatggtt taagattaag tgatttgcct
     9421 atatcatggt ataaccaagg acaaggaagc aagcttgcaa aagcgatggc taagttcggt
     9481 tctgcaaatt gcctataaat atgagaaatg taatgataac aggttggagc gattggatgt
     9541 tgctccattt cctggtcgtt gatttccaat tccttgaaaa attgttggcg aataaataac
     9601 tcaccctcca ctaaaccctg agcattttgt ttcaagagtc ttttcatcac ttggtttgaa
     9661 gtcttatcag ccaaaagatg atagatttct gagaaggcct tcagatagta ggcatcctga
     9721 atcaggtaat agcggaaaat ggcaggttct aaattcccct cttgtaattg taaaataaag
     9781 ggatgatgaa aggaagcctg ccaagctttc ttggataatt ccatcgcaat atctgtaaat
     9841 tccataataa ctcctttata aaaatagact ggtttgaagc aataaaaaga aaagcaggta
     9901 gattaatttt gtttttttag gaatataaaa agtccgatag ctattcttca actgtgcatg
     9961 ttcgtcatat ccatgagcag acagagctct caggtaaaga tggcgccacc taaagactgt
    10021 catcagaacc ttactgtaaa tcaagggcga ccaaaaatgt agttcttgac cacgtaatag
    10081 gcaagcttct ttgagggact tgatttcttg ctgaatgaga ggaaaggaat tgaataccac
    10141 aatcaaggca taggaccaag agcgtgatag ccccttttga gccaagtaca agagaagctc
    10201 ttttagtgaa atagaggaaa caaagacaag gccgatacaa actgtcacaa aggccctcgt
    10261 tccaagcatg actgcctgtg aagcatctcc gtgtaactga actgcccagt agttggcaaa
    10321 agatggtaaa atggcaagta tgatcatcca agctaacatt ttaaatcgac ggtaatagag
    10381 cataaagaga atacaaaatg cgactaccga aagattcaga gcaatcgaag gaatgaaaga
    10441 tgtttccaag gataaaatca gcaagaagag actgataatc ggcgtctggg ttgctacttt
    10501 gaccatacta tctcacctcc ccttgggtat tgctactctg agatgtaagt ggtttggtaa
    10561 tggtcacttc tttcacatgc cgaagcccct gactagtcat ctcaatccaa taatcaacca
    10621 cagaaatcaa agggtctaaa cgatgactaa tgagcagaaa acttcttcct tgattcctct
    10681 cctccacaat ccacttgcaa aaataatggc aggctctatc atccaaacct gcaaaaggtt
    10741 catctagcaa gatcacggaa gccttactgg tcaagatggt caagagctga agaatttttt
    10801 gctgaccacc acttaattga tagggactct tatcgactgc ctgctccaaa tcaaaatatc
    10861 gtaaagcttg aaaaatccgc tgatttcttt cagagtctgt actatctaat tgcagttcct
    10921 ctcgcagact gactcggata aactgcttct cagcttcctg aacaacacca gtcagatcac
    10981 gatacaaact ctttttcttt ttcaggaccg aacccttcca agtaatgctc cccttatact
    11041 tttgaaattg aagaatagag cgaaagaagg ttgatttccc gacaccattg tcacccagga
    11101 tacaggaaat cccttgatag aaagtaaaat tcgcaatcga aaagaggggg cgattaccaa
    11161 gctcaccaat cacacggttc atatggaata gttccgggct agaagcaact ttctttgaag
    11221 taacctgtat catctcatag gaagggattt gaaacacttc ccttagtttt ccgtctctta
    11281 gctcaaccat atggtcgata taggctttat agtcagataa atcatggtcg cacaaaataa
    11341 ctgtcttccc atcatagacc aactctttta gaatctccaa tatctcgatt ctgctcttgc
    11401 ggtcaatgga agcgaagggc tcatccaaga gatagaccct aggattcatg gcaaagagga
    11461 tagccagcgc tgctttttgc ttttccccac ctgataggtg atgaatggga cggtgtaaga
    11521 ttgcctcgca acgacattgc tggacaacct ctgctatttt agaatcaatt tcctgaaggt
    11581 gatagccgat attttccatg gtaaaaacca actcctcaaa caagctctcc atggtaaatt
    11641 gatgattagg attttgcaag agaataccaa ccgtctggac acgttcgacg atagaaatct
    11701 gattgacctc gctcccatct atcaggactt gaccgctata gggaagagaa ctaacttggg
    11761 caatcatttg aaagaggctg gattttccag acccactact cccaactaac aaggtaaagg
    11821 cttgcgcatg aaaagtaaaa tcaaacggct cagagaagat tggggactga atcgctcgta
    11881 gttccagacc catctatgct tttcctccag ttgcaaactg atgatagagt ttgacaatgg
    11941 cacgaaccaa gatggtacag aagaaataaa cagaaataaa acgtaccaca agcaaggaaa
    12001 ggacaaacgg aagggaaaag gcgtagtaac ctaacttaat gtattcatag acaaagctaa
    12061 caagcgtaat cccaatacta ttagcagtta gagagagcca actttcatag cgattcttag
    12121 ttacgataaa accaaattca cttcccaaac cttgaaccaa gccagacaaa agagctccta
    12181 gaccaaattg gctaccataa aggacttcag caagcgcagc tagcacttct ccaatcgttg
    12241 cacttccgac tctcggaaca aagatggcag caatgggcgc agccatacac cagagaccga
    12301 agaggatttc attggcaaag gcctgcaaac caagaggtgt taagagtaga ctgagaatat
    12361 tatacacata tcctgaacca acgaaaaccc caccaaaaaa gatagacaag aaagcaagca
    12421 agataacatc ttttaactgc cattttttca acataaaaaa ctcctttttt aaagaaaagt
    12481 ggggcactca agaagaccga cctaaatact ttgtatagta gattgaattt agaacagtac
    12541 acaagaatac taaaatattt ctagagatta atttgaattt tctaattaat ttgttcgcat
    12601 cttatttcaa tctactatat catcttcatc cagtttcgta aaagaaaaaa ctctaattac
    12661 agatacgaat tagagttcag cttacaagat tagacggttc ttttcgacat acgaaaaaaa
    12721 catttcacat ttcccttcgc cagtcttaac tg
//
LOCUS       DAVGJJ010000055        12415 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000055 DAVGJJ010000000
VERSION     DAVGJJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.055"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..266
                     /locus_tag="VVE35_001413"
     CDS             <1..266
                     /locus_tag="VVE35_001413"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061591.1"
                     /translation="FKKAYFKEVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVKKD
                     AETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTDSFKTSIFR"
     gene            327..2048
                     /gene="pabB"
                     /locus_tag="VVE35_001414"
     CDS             327..2048
                     /gene="pabB"
                     /locus_tag="VVE35_001414"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU3061592.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVAEVADLLAQVESYQ
                     EQDYYVVGYVSYEAAPAFEEKLAVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAENYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLGQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTIWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTVQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            2049..2666
                     /locus_tag="VVE35_001415"
     CDS             2049..2666
                     /locus_tag="VVE35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU3061593.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            2716..3706
                     /locus_tag="VVE35_001416"
                     /pseudo
     CDS             2716..3706
                     /locus_tag="VVE35_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            3841..4800
                     /locus_tag="VVE35_001417"
     CDS             3841..4800
                     /locus_tag="VVE35_001417"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU3061594.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            4898..5737
                     /locus_tag="VVE35_001418"
     CDS             4898..5737
                     /locus_tag="VVE35_001418"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU3061595.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(5785..5955)
                     /locus_tag="VVE35_001419"
     CDS             complement(5785..5955)
                     /locus_tag="VVE35_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU3061596.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            6076..6960
                     /gene="miaA"
                     /locus_tag="VVE35_001420"
     CDS             6076..6960
                     /gene="miaA"
                     /locus_tag="VVE35_001420"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU3061597.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            6953..8191
                     /gene="hflX"
                     /locus_tag="VVE35_001421"
     CDS             6953..8191
                     /gene="hflX"
                     /locus_tag="VVE35_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU3061598.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            8184..8807
                     /locus_tag="VVE35_001422"
     CDS             8184..8807
                     /locus_tag="VVE35_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU3061599.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            8822..9751
                     /locus_tag="VVE35_001423"
     CDS             8822..9751
                     /locus_tag="VVE35_001423"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU3061600.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            9772..10527
                     /locus_tag="VVE35_001424"
     CDS             9772..10527
                     /locus_tag="VVE35_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU3061601.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(10581..11489)
                     /locus_tag="VVE35_001425"
     CDS             complement(10581..11489)
                     /locus_tag="VVE35_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU3061602.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            11553..11822
                     /locus_tag="VVE35_001426"
     CDS             11553..11822
                     /locus_tag="VVE35_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU3061603.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            11822..12202
                     /locus_tag="VVE35_001427"
     CDS             11822..12202
                     /locus_tag="VVE35_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU3061604.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         3669 a         2370 c         2855 g         3521 t
ORIGIN      
        1 aatttaaaaa agcttatttc aaagaagtta aagataaagc aacgaaagga ttaacaacat
       61 tcgaagtaaa tggttcttcc gtttcatcat acgatgattt acttacacta tttaaagaag
      121 ctgttaaaaa agatgccgaa actcttaaac aagaagcaaa cggtaataaa acagtatcta
      181 tgaataatac agttaaatta aaagaagctg tttataagaa acttcttcaa caaacagata
      241 gctttaaaac ttcaatcttt agataatcga aatctctcat ctgctttgcg gatgagggtt
      301 ttcttatttt atgctatact taaggtatgc atagaaaaac agtgattgat tttaggactt
      361 taggggagag atataccttt acccagccta ttaaagagtt gaaaacgaga aatgtagcag
      421 aagtggcaga tttgctggca caagtggaaa gctaccaaga gcaagattat tatgtggtgg
      481 ggtatgtcag ctacgaggct gcacctgctt ttgaggagaa attagcagtt cacaaggttc
      541 ctctactggg cgagtacttg ctttacttta ctgttcacga tagggtggag acctccccta
      601 ttcctctgac ttatgaggat attgatttgc cctcaaattg gcaggaagta acgtctgcag
      661 agaactatga aaaggccatt gcccagatac accatcactt gaggcaggga gacacctatc
      721 aggtcaacta caccgtccaa ctcaagcaaa agttaagtgc caatcctttt gccatctaca
      781 atcgtatggt ggtagagcag gaggcgggct acaatgccta tgtggaacat gacgagatgg
      841 cagtgatttc catgagccca gagctctttt ttgagcaaaa tgatcgcgag ttgacaacac
      901 gaccaatgaa ggggacgact cagcgtgggg taactgacca agaagatctt gaacaggcca
      961 gttggttggg acaggatccc aaaaatcgct ctgaaaatat gatgattgtg gacctcttgc
     1021 gcaatgatat gaaccgtatt tctgaagttg ggagcgagca cgtggagcgt ctgtgccagg
     1081 tagagcagta ttcaactatt tggcagatga cttcgaccat caagagtcag ttgcgagagg
     1141 atgtggacct tgttgaaatc ttccgctcac tctttccttg tggttccata acgggtgcac
     1201 cgaaaattgc gacaatggag attatcaagg acttggagcc tcaaccgcgt ggagtctact
     1261 gtggaacgat tggtctcttg cttccaaatg gacgacggat ttttaatgtg gccattcgta
     1321 ccgttcaact tcacaaaggt caagctattt atggagttgg cggagggatt acttgggata
     1381 gcacatggga atctgaatac cgagaggttc atcaaaaggc agcagttctt tatcgtaaac
     1441 aagctcgctt ccaactgatt acgacagggg aaatcagcca gaagaacctg ctgtttgaag
     1501 atcaacatct ggaaagactg agaaaagcta gtcgttattt tgcctttcct tttgatgcag
     1561 aagacttggg acacaagatt gaggaagagt gtcaggattg tgaagctaat cgagattacc
     1621 gcttgcgaat cagccttagc aaatctggag agatagaagt caatcgtcaa gtattaaccc
     1681 ctctcagtac aagcttttgt caggcccaag tctgccttca ggaagctgct ttgaatcaat
     1741 cctttaccta ctttaaaacc actcaccgac cgcatttgag cctaggagaa caagagaaga
     1801 tttaccacaa taagtcagga gaactgcttg aaacctctat aggaaatttg gttctgaaaa
     1861 tcgctggaaa actctacaca ccgcctatcc gacttggaat cttgccagga atttaccgtc
     1921 agtatttgct agaaacagga caggtagaag agaaagtctt gaccttggca gatttagccc
     1981 aagcagaagc tatttacggc tgtaatgcag tgagaggctt gtatgaacta agccttgagg
     2041 agaactagat gaaattgata tttttgcatg ggttagggca gtcagcagag tcttggaaag
     2101 aagttcggaa tctactgaca gattatcctt ctgaggctat cgagttattt ccttctggag
     2161 ttagtaacta tcaacaagct aaagagcgag tttatcagca cctagctcaa gagacagaac
     2221 cttttgtttt gattggattg tccttaggtg ctgccctgac actagaactt tccagttacg
     2281 atttaccaaa tcttcgagct ttgattctgt caggatgccc gcttaaatta gcagggaata
     2341 tcctatttta tcttcaattg ctgattttta aactacttcc caaaagagta tttgaaaaac
     2401 agggagcaga caagactctt atggtcggag tttctgagga attgaagaca cttgatttaa
     2461 cggatatagc agggacttgc tcctatccaa ccttactaat ttgtggcagc aaagataaac
     2521 cgaatcttag ttctatgaaa gctctccata gactgttaac agattcgcag tttcagatca
     2581 ttcctgatgg ccctcatgtc ttgaataagg caaaaccaaa ggagtttgta gaaaagacca
     2641 gaagtttcct tgaattgctg aaataagttt gataaaataa tattgaaatc gatatcataa
     2701 ttatgggaga aagaaatgaa taaacgtcta tttttaaaaa tgagtctggt gacgttgcca
     2761 attttagcct tgttttcaca accagttttg gcggaagaaa acatccattt ttcgagctgt
     2821 aaggaagctt gggcgaatgg ctattcggat attcacgagg gggaacctgg ttattctgcc
     2881 aagttagacc gtgatcatga tggtgtggct tgcgaattga aaaatgctcc taagggtgct
     2941 tttaaagcaa aacagtcaac ggctattcaa atcaacacaa gttcagcaac aacaagtggt
     3001 tgggttaagc aggacggcgc ttggtactac tttgatggaa atggaaatct agtgaaaaat
     3061 gcatggcagg gaagctatta cctgaaagct gatggtaaaa tggcacagag tgaatggatt
     3121 tatgactctt cttatcaagc ttggtattat ttgaaatcag atggttctta tgcaaaaaat
     3181 gcatggcaag gagcttatta ccttaaatca aacggtaaaa tggcacaagg tgagtgggtt
     3241 tatgattctt cttaccaagc atggtattac ttgaaatcag atggttcata tgctcgcaat
     3301 gcatggcaag gaaactacta tttgaaatca gatggtaaaa tggctaaagg tgaatgggtt
     3361 tatgatgcca cctatcaagc ttggtattat ttgacatcag atggttctta tgcttacagt
     3421 acatggcaag gaaattacta tctaaaatcg gatggtaaaa tggctgtcaa tgaatgggtt
     3481 gatggtggac gttattatgt tggcgctgac ggagtttgga aggaaggtca agcaagtaca
     3541 gcttcttcta gtaatgatag caatagtgaa tattctgctg ctttaggaaa ggcaaaaagt
     3601 tataattcgt tattccacat gtcaaaaaaa acgtatgtat agacaattaa cttctgattt
     3661 tgataaattt tcaaatgatg cagctcaata tgccattgat cattagatga ttaacagata
     3721 ctggtaggcg aaaaaattcc ttataattca cttacgcaaa cctttgcata agtgaatgga
     3781 ttttgatata ctagtactgt aagcattttc aattactaca caatgaaaaa ggagaatatg
     3841 atgagtcaaa agattattgg gattgacctt ggtggaactt ctatcaaatt tgcaatctta
     3901 acaacagcag gagaaatcca aggaaaatgg tcaatcaaga ccaacatttt ggatgaggga
     3961 agtcatatcg ttgatgatat gattgagtct attcaacatc gtttggactt gcttggattg
     4021 gcagcagcgg acttccaagg cattggaatg ggatcaccag gtgtggttga ccgtgacaaa
     4081 gggactgtta tcggtgccta caacttgaac tggaaaaccc ttcaaccaat caaacaaaag
     4141 attgaaaaag ccttgggcat tccatttttc atcgataatg atgccaacgt agcagctctt
     4201 ggtgagcgct ggatgggtgc tggagataac caaccagacg ttgtctttat gacactcggt
     4261 actggtgttg gtggtggtat tgtcgcagaa ggcaaattgc ttcatggtgt tgctggtgca
     4321 gcaggtgaac ttggtcatat cactgttgat tttgaccaac caattgcatg tacttgtggt
     4381 aaaaaaggct gccttgagac agttgcttca gcaacaggaa ttgtcaactt gactcgtcgc
     4441 tatgccgatg aatacgaagg cgatgcagcc ttgaaacgct tgattgataa cggagaagaa
     4501 gtaactgcta agactgtctt tgatctcgca aaagaaggag acgaccttgc tttgattgtt
     4561 taccgtaact tctcacgtta cttgggaatc gcttgtgcta acattggttc aatcctaaac
     4621 ccatcaacaa tcgtcatcgg tggtggtgtg tcagctgcgg gagaattcct tctacaaggt
     4681 gttcaaaaag tctatgatga aaatactttc ccacaagtac gcacatctac taaattagct
     4741 cttgcaactc taggaaatga cgctggagtt atcggagcag catcacttgt attgcaataa
     4801 gataatgaga gaggaaaaat gctaacctag agttagtaat gttcctcttt tattttttat
     4861 gctatactag ttaggacaat gaatgaggtg agagtaaatg acaaaagcag atacgatttt
     4921 taaagagaat attgaacgca ttctaaagga aggtgttttc tcagagcagg ctcgtcctaa
     4981 gtacaaggat gggactgttg ctaattccaa gtatgtaact ggtgcctttt ccgagtacga
     5041 cttatctaaa ggagaatttc ctatcacaac cttgcgtccc attgcaatca aatccgccat
     5101 caaagaagtt ctctggattt accaagacca gtcaaatagc ttagaagtgc ttaatgacaa
     5161 gtacaatgtt cactactgga atgactggga agttggagac acgggaacca ttggtgagcg
     5221 ctatggtgcc gttgttaaga aacacgacat tatcaataag cttctcaaac agttggaaac
     5281 caatccttgg aatcgccgca atattatttc gctctgggat taccaagctt tcgaagaaac
     5341 agatgggctg ctcccgtgcg cctttcagac catgtttgat gtccggcgtg ttgatgggga
     5401 aatctatctg gatgcgacct tgacccagcg ctccaatgat atgctggtgg cccaccacat
     5461 caacgctatg cagtatgtgg ctttacagat gatgattgcc aaacattttg gttggaaggt
     5521 tgggaagttc ttctatttca tcaacaacct ccatatctat gataatcaat ttgaacaagc
     5581 tcaggaattg ctccgtcggg agccgtcaaa ctgccaacca cgcttggttt taaatgttcc
     5641 tgatgggact aatttctttg atatcaaagc agaagatttt gagttggtgg attatgaccc
     5701 tgttaagcca cagttgaagt ttgacctagc tatttaaaag aatagaaaaa agaagttgag
     5761 aataatccca acttcttttg tttcttaacg tgatacgcgg cgacgagctg cttttttacg
     5821 attttcttcg atgaaagctg ctttttgctc ttctggttcg attactttct ttttaaatgc
     5881 gtatactgca cctgcaacgg cagcgacagt tcctgcgaca cctgttacaa gacctttagc
     5941 gaatccttta gccatgagtc ttcctccttt atattctcaa tcagccagcc tcctcaagag
     6001 gtcacatttt tctgactgac ctttttgtgt tataataata gtaacgaaaa aatgggaatt
     6061 tttcaaggaa aaaagatgaa aacaaaaata attgtgattg ttggaccgac tgctgttgga
     6121 aagacggctc tagctattga agttgcaaag cgctttaatg gcgaagtggt tagtggagat
     6181 agccagcaag tctatcgagg acttgatatt gggacggcca aggctagtcc agaagagcag
     6241 gcagctgttc ctcatcattt aatcgatgtt agagagataa ccgagtctta ctcggctttt
     6301 gattttgttt cagaagctaa gatgactatt gaggatattc agagccgtgg caagctagcc
     6361 attattgccg gtgggactgg actttatatc cagagcttgc tagaaggtta ccacctaggt
     6421 ggggagactc ctcatgagga gattttagct tatcgagcta gtttggagcc atattcagat
     6481 gaggaattag cccatttggt ggagcaagca ggccttgaga ttccccagtt taatcgtcgt
     6541 cgtgctatgc gtgccttaga aattgcccat tttggtcagg atttggaaaa tcaagagatc
     6601 ttgtatgaac cgctgattat ctgcttggat gatgaacgta gtcaacttta tgagcgtatc
     6661 aaccaccgag tggatttgat gtttgaggct gggcttttgg atgaggccaa gtggctgttt
     6721 gaccattccc ctaatgtaca ggctgctaaa ggaattggct ataaggaact ctttccttat
     6781 ttccgtggag agcagacctt tgaggaagct cgtgagagtc ttaaacaggc gacccgtcgt
     6841 tttgccaagc gtcagctgac ctggttccgt aatcgcatgc aggtcacctt ttatcagatt
     6901 ggagaatctg gtgtacaaga ccgcatttta agccagatag aggagttttt agatgattga
     6961 aacggagaaa aaagaggagc gagtcctgct gattggtgtg gaattgcagg gtatggacag
     7021 ttttgacctc tccatggaag aattggctag tttagcgaaa acggcagggg cagtcgttgt
     7081 agatagctac agacaaaaac gtgaaaaata tgattccaag accttcgtcg gctctggtaa
     7141 gttggaagag attgcgctta tggtggatgc agaagaaatc actactgtca tcgtcaacaa
     7201 ccgtctgacc ccaaggcaga atgtcaatct agaggaagtt ctcggtgtta aggtcattga
     7261 ccgtatgcag ttgattttgg atatctttgc catgcgggct cgaagccatg aagggaagct
     7321 ccaagtccac ctagcccaac tcaaatacct cttgcctcgc ttggttggtc aggggattat
     7381 gctcagccgt caggcagggg gaattggttc ccgtggtcct ggtgaaagcc agctggagct
     7441 gaaccgtcgt agcgttcgca atcaaatcac ggatatcgag cgccagctca aggtggttga
     7501 gaaaaatcgg gcgactgtca gagaaaaacg tttggagtct agcaccttta agattggttt
     7561 gattggttat accaatgctg ggaaatcaac tatcatgaac atcttgacca gtaagaccca
     7621 gtatgaagca gatgagctct ttgcgactct ggatgcgaca accaagagta ttcatctggg
     7681 aggcaatctc caagtaactt tgacagatac cgttggcttt atccaagatt tgccgacaga
     7741 gttggtgtcc agtttcaagt caaccttgga agaaagcaag catgtggacc ttctggttca
     7801 tgttatcgat gctagcaatc cttaccacga ggagcatgaa aaaacggttc tctccatcat
     7861 gaaagacctg gacatggaag atattcctca cttgacgctt tataataaag cggatttggt
     7921 ggaggatttc acgcctactc aaacgccata taccctcatt tctgccaagt ctgaggacag
     7981 tcgtgaaaac ttgcaagcat tattgctaga taagattaag gaaatttttg aagcatttac
     8041 cctgcgagtg cctttttcaa agtcctacaa gattcatgat ttagagagtg ttgcaattct
     8101 ggaagaacgt gattatcagg aagacggcga agtgattaca ggctacattt ccgagaaaaa
     8161 taaatggagg ttagaagaat tttatgactg atattaaaac tttggctcta aagtatggag
     8221 gttatacaag tctggacaag gtctatctgg atcagcttct agctggtaaa acagagcagg
     8281 agcagttaac acttattaca cctccgccaa gtgttgttaa tgcctacttt gcagaactct
     8341 atcagaaaaa aagtcctcag gctgcgacgg attattttgc agaactcagt caggaactga
     8401 atctttacaa tactgagcca agtttcaccc tagaaaataa gccttttatt cggcttaatc
     8461 tgtctggtaa atcctttggt ttttgttatg agagtgacgg ctttggtcgg attttctctg
     8521 aaaatgagga gaaaatctcg gatgacttgc tctttgaaat tgcgcaaatt ttctcccatc
     8581 aattagtctt tgaagagtct ggtaagattt acatgaagcc tgtcaaggac gaggaagttg
     8641 tcagcctaga aagtctcaca gctttgactg atttggaaag attggctgat ggtcgaaaac
     8701 gtctcaaagg ctacagccaa gaggatttat tgcaagaagc tgctgctttt tctggcaaac
     8761 gctatttccg atcggaaaac cgcacagcca tgttatatat tgattaatta gaaagtatcg
     8821 aatggatatt caatttttag gaacgggggc tggtcagccc tctaaagccc gcaacgtttc
     8881 aagtctcgcc ctgaaacttt tggacgagat taacgaagtt tggctctttg actgtggaga
     8941 aggtacgcaa aatcgcattc tggaaaccac aattcgacca cgtaaggtca gcaaaatctt
     9001 tattacccat ctgcatggag accacatttt tggtttgcca ggtttccttt ctagccgtgc
     9061 ctttcaggcc aatgaagagc agacagattt ggaaatctac ggacctcaag gaatcaagtc
     9121 atttgtctta accagccttc gtgtgtcagg ttctcgtctg ccctaccgca ttcatttcca
     9181 tgagtttgac caagattctc tgggtaaaat tcttgaaacc gataaattca ctgtgtatgc
     9241 agaggagctg gaccacacta ttttctgtgt tggctatcgt gtcatgcaaa aggatctaga
     9301 agggacgctg gatgctgaaa aactcaaggc tgctggtgtt ccgttcggcc cgctttttgg
     9361 taaaatcaaa aacggccagg atcttgtttt ggaagacgga actgaaatca aggcagcaga
     9421 ctatatctca gcgccacgtc caggtaagat tatcactatt ttaggagaca ctcgaaaaac
     9481 ggatgccagt gtgcgtctgg ctgtcaatgc agatgtccta gttcatgagt ccacttatgg
     9541 caagggtgat gaaaaaattg ctcgtaacca tggtcactca actaatatgc aagctgcaca
     9601 agtagcggta gaagcaggtg ccaaacgcct cctactcaac catatcagtg cccgtttcct
     9661 ctcaaaagat ataagcaaac tcaagaagga cgctgccaca atttttgaaa atgtccatgt
     9721 ggtcaaagac ttggaagaag tggaaatcta gcagtcacag aaaggataag tatgcctact
     9781 attctcatta ccggagctag cggtggtcta gctcaagaaa tggtcaaact cctgcccaat
     9841 gaccaactca tcttgcttgg tagaaataag gaaaaattag cccaactcta cggaaattat
     9901 tcccatgcag aattgattga aattgatatt accgacgatt cagccctaga agctctggta
     9961 actgatcttt atctccgcta tggcaagatt gatgtcttga ttaacaacgc tggttacggg
    10021 atttttgagg gatttgacca gattgctgat aaagatattc accagatgtt tgaggtcaat
    10081 acctttgccc tgatgaatct gtctcgtcac cttgcggctc gtatgaagga aagcagcaaa
    10141 gggcatatca tcaacatcgt cagcatggca ggtctaatag ctactggcaa gtctagtctt
    10201 tactcagcga ccaagtttgc ggctattggt ttttcaaatg ctctgcgact cgaacttatg
    10261 ccctatggag tctatgtgac aacagtcaat ccaggtccaa tccgaacagg attttttgac
    10321 caagctgacc cagatggaac ttatcttaaa tcggttgacc gcttcctctt agaggcagat
    10381 gcagtggcta aaaagattgt caagattata ggcaaaaata aacgagaact caatctcccg
    10441 attttgttga acctagccca taagttttat actctctttc ccaagctagc tgataagttg
    10501 gcaggggaaa cttttaatta taagtaaaaa gaaccaatgt gcaggttgtt gctagcctac
    10561 atattggttc ttatcatttc tcaactatca aactgaactt cttctagtag atagtcgata
    10621 aagcgttcgc ccatcttaga taggctggtt ttctcatgct ggatatagac cagctcaatc
    10681 gggtcatcaa tatccagtgg gatagaaacg atattgtctc cgtttaggtt gctgttcaaa
    10741 atccctgtcg caatggtata accatccaaa ccaatcaaga gattaaagag ggtggcacgg
    10801 tcactgacca caatggattt cttgtggtgt tcctgagaaa gaatctcttc tgaaaagtag
    10861 aaggagttgt gcgtcccttg gtcatagctg aggtaaggga aattctccaa atcagacagt
    10921 ttcaccttgt ctttctttgc cagagggttg gtcttgctga caaagatatg cggttgcgct
    10981 gtgaagagat ggtgggctag caggtgattg tcatccagca tcttggttaa aacatcacgg
    11041 ttgtaactgt ttaagaagag gaccccgacc tcactgcgga agttcttgac gtcgtcgata
    11101 atctcccaag tccgagtttc acgaaggaag agttcgtatt tctccatatc gcttttcttg
    11161 agcaaagaga caaaggcatt gaccacaaag gcatagtgtt gagacgaaac gctaaagagt
    11221 tcgcggtggg cgacaggatt tttatagcgt tcctccagaa gctgggtctg ctcgacaacc
    11281 tgacgggcat aagagagaaa ctccatccca tcacgggtca aggtgattcc cttgggattg
    11341 cggataaaga tctcaatgcc catttcattt tccaaatctc gcactgcatt ggagagactt
    11401 ggctgagtga taaagagttg cttggctgcc tcattcatgg agccagtttc gacgattttg
    11461 ataatataat gtaattgttg aattctcatg tttttattgt accatacttg ccttagaatc
    11521 gctaaagaag ataggaaaat aaggagcatt gtgtgagtct cttcttgaaa aaaacacaaa
    11581 attttgctag aatgagtctt atgaaaacat tctatgatgt gcagcaattc ctcaaacgat
    11641 ttggtattat tgtttacatg ggaaaacgct tatatgatat tgaactgatg aagttggaac
    11701 tatctcggat ttacgatgca gggttgatgg ataaattaga ctatctagag gcagaagcag
    11761 ttcttcgcag agagcacaag gtagaattgg actatattga gaaaaatgga gaaaagaact
    11821 aatggttact tggattttgt gggcacttat actagcaatg ttggcgtgga tgggctttaa
    11881 ctatcttcgt attcgccgtg cggctaaaat tgtggacaat gaggagtttg aagccttgat
    11941 tcgtacgggt caattgattg atttgcgcga cccagcagaa ttccacagaa aacatatcct
    12001 tggtgcacgc aatattcctt caagtcagtt gaaaactagt cttgcagccc ttcgtaaaga
    12061 taaacctgtc cttctctacg aaaaccaacg tgcgcaacga gttacaaatg cagctcttta
    12121 cttgaaaaaa caaggttttt ctgagattta tatcctttct tatggcttgg attcttggaa
    12181 agggaaagtg aagactagct aaccttattt ccaaattagt cgaatgatag gatgaagcta
    12241 ttgttttata gtataattaa tttgtataaa atttaaagag ttcttagaac atgaaagaaa
    12301 agaagaaaaa tccacttttt gtgggtatct tgtcgattat tcttggtttg ttatttccaa
    12361 ttgtaggctt gattttgggt atcataggat tggtcttggc tatttcatac caaaa
//
LOCUS       DAVGJJ010000056        12189 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000056 DAVGJJ010000000
VERSION     DAVGJJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.056"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            128..1357
                     /gene="arcA"
                     /locus_tag="VVE35_001428"
     CDS             128..1357
                     /gene="arcA"
                     /locus_tag="VVE35_001428"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU3061605.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1415..2431
                     /gene="argF"
                     /locus_tag="VVE35_001429"
     CDS             1415..2431
                     /gene="argF"
                     /locus_tag="VVE35_001429"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU3061606.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2596..3543
                     /gene="arcC"
                     /locus_tag="VVE35_001430"
     CDS             2596..3543
                     /gene="arcC"
                     /locus_tag="VVE35_001430"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU3061607.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            3752..5263
                     /locus_tag="VVE35_001431"
     CDS             3752..5263
                     /locus_tag="VVE35_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU3061608.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESILSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            5285..6616
                     /locus_tag="VVE35_001432"
     CDS             5285..6616
                     /locus_tag="VVE35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU3061609.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLYGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7022..7312)
                     /locus_tag="VVE35_001433"
     CDS             complement(7022..7312)
                     /locus_tag="VVE35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061610.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(7300..7552)
                     /locus_tag="VVE35_001434"
                     /pseudo
     CDS             complement(7300..7552)
                     /locus_tag="VVE35_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            7756..7833
                     /locus_tag="VVE35_001435"
                     /pseudo
     CDS             7756..7833
                     /locus_tag="VVE35_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7951..8835)
                     /locus_tag="VVE35_001436"
     CDS             complement(7951..8835)
                     /locus_tag="VVE35_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU3061611.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(8980..10131)
                     /locus_tag="VVE35_001437"
     CDS             complement(8980..10131)
                     /locus_tag="VVE35_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU3061612.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(10277..12043)
                     /locus_tag="VVE35_001438"
     CDS             complement(10277..12043)
                     /locus_tag="VVE35_001438"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU3061613.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEALESADYRACQLLGPLHK"
BASE COUNT         3586 a         2557 c         2372 g         3674 t
ORIGIN      
        1 aacatcataa tttatattat tttccgagaa attatagcca ttgacattac ctaggaattg
       61 cgttataaca tacatgtaag cggtacccaa aatggtgcca agtcaaaatt tttaaggagg
      121 aaaatacatg tcttcacatc caattcaggt cttctcagaa attgggaaac tgaaaaaagt
      181 tatgttgcac cgtccaggca aggagttaga aaacttgttg ccggactatc ttgaaaggct
      241 tctttttgat gatattcctt tcttggaaga tgctcaaaaa gaacatgatg catttgccca
      301 agctcttcgc gatgaaggaa ttgaggttct ctacctagaa caactcgctg ctgaatcatt
      361 gacctctcca gaaatccgcg atcaatttat cgaggaatac ttagacgaag ccaacatccg
      421 tgatcgtcaa accaaggttg ctattcgtga attgcttcac ggcatcaagg acaaccaaga
      481 attggttgaa aaaacaatgg ctgggattca aaaagttgaa ttgccagaaa ttcctgacga
      541 agctaaagat ctaactgact tagttgaatc agattatcca tttgcaattg acccgatgcc
      601 aaacctctat ttcactcgcg acccatttgc aacaattgga aacgccgtat cgcttaacca
      661 catgtttgca gacactcgta accgtgaaac actctacggt aagtatatct tcaaatacca
      721 cccaatctat ggcggaaaag tggatttggt ctacaaccgt gaagaagata cgcgtatcga
      781 aggtggagac gagctagttc tttctaaaga cgtccttgca gtaggtatct ctcaacgtac
      841 agacgcagct tctatcgaaa aacttttggt caacatcttc aagaaaaatg ttggcttcaa
      901 gaaagttttg gcctttgaat ttgctaacaa ccgtaaattc atgcacttgg atactgtctt
      961 cactatggta gactatgaca agttcactat tcacccagaa atcgaaggcg accttcacgt
     1021 ttactcagtt acttacgaaa acgaaaaact taaaattgtt gaagagaaag gtgacttagc
     1081 tgaacttctt gctcaaaacc ttggtgtaga aaaagttcat ttgattcgtt gcggtggtgg
     1141 caatatcgta gcagctgcgc gtgaacaatg gaacgacggt tctaacacct tgaccatcgc
     1201 acctggtgtg gtagttgttt atgaccgcaa taccgtgacc aataagattt tggaagaata
     1261 cgggcttcgc ttgattaaga ttcgcggaag tgaattggtt cggggccgtg gtggacctcg
     1321 ttgtatgtct atgccatttg aacgtgaaga agtgtaatcg ctgttcggca ttcgtcaata
     1381 gaaaatgtaa aaaatagaaa gaggaaataa taaaatgaca aattcagtat tccaaggacg
     1441 cagcttctta gcagaaaaag actttacccg tgcagagtta gaatacctta ttggtctttc
     1501 agctcacttg aaagatttga aaaaacgcaa tattcaacac cactaccttg ctggcaagaa
     1561 tatcgctctc ctatttgaaa aaacatctac tcgtactcgt gcagccttta caactgcggc
     1621 tatcgacctt ggtgctcacc cagaatacct cggagcaaat gatattcagt tgggtaaaaa
     1681 agaatctact gaagatactg ctaaagtatt gggacgtatg tttgacggga ttgaattccg
     1741 cggattcagc caacgtatgg ttgaagaatt ggcagaattc tcaggcgttc cagtatggaa
     1801 cggtctaact gacgaatggc acccaactca aatgctcgct gactacttga ctgttcaaga
     1861 aaacttcggt cgcttggaag gcttgacatt ggtatactgt ggtgatggac gtaacaacgt
     1921 tgccaacagc ttgctcgtaa caggtgctat ccttggtgtc aatgttcaca tcttctcacc
     1981 aaaagaactc ttcccagaaa aagaaatcgt tgaattggca gaaggatttg ctaaagaaag
     2041 tggcgcacat gttctcatca ctgaagatgc tgatgaagca gttaaagatg cagacgttct
     2101 ttacacagac gtttgggtat caatgggtga agaagacaaa ttcgcagaac gtgtagctct
     2161 tcttaaacct taccaagtca atatggactt agttaaaaaa gcaggcaatg aaaacttgat
     2221 cttcctacac tgcttgccag cattccacga tactcacact gtttatggta aagacgttgc
     2281 tgaaaaattt ggtgtagaag aaatggaagt aacagacgaa gtcttccgca gcaagtacgc
     2341 tcgccacttc gatcaagcag aaaaccgtat gcacactatc aaagctgtta tggctgctac
     2401 acttggtaac ctttatattc ctaaagtata attttagata ataaaccgtc taccaacagc
     2461 tatgagggct gcgactaata gctttagtcc ggtcctcttt tatgtaatgg taatctatta
     2521 tttcttataa aatatgtgaa aaatcattaa attgaaatct aaacgcattc tattgagtgt
     2581 gataaaggag aatttatggc aaatcgtaaa attgtagtag ctttgggagg aaatgcgatt
     2641 ctttcttctg acccatcagc aaaggctcaa caagaagctt tagttgaaac agctaagcat
     2701 cttgtaaaat tgattaaaaa tggagatgat ctgattatca ctcacggtaa tggacctcaa
     2761 gttgggaatc tcttgctcca acatttggca tcagactctg aaaagaaccc tgccttccca
     2821 ctcgactcac ttgtcgctat gacagaaggt agcatcggtt tctggttgaa aaatgctttg
     2881 caaaatgctc tcttggatga aggcatcgaa aaaaatgttg cctctgttgt aacgcaagtt
     2941 gtcgtagata aaaatgatcc agcttttgtt aacttgagta aaccaatcgg tcctttctat
     3001 tcagaagaag aagcaaaagc agaagccgaa aagagcggag cgactttcaa ggaagatgct
     3061 ggccgtggct ggcgtaaggt cgttgcctca ccaaaacctg ttgacatcaa agaaattgaa
     3121 accatccgta ctcttttaaa tagtggtcaa gtcgtcgtag ctgcaggtgg tggcggtatt
     3181 cccgtcgtca aagaaaacaa tggacatttg actggtgtcg aagcggttat tgataaagac
     3241 ttcgcttccc aacgtttggc agaattggtt gatgcagacc tcttcatcat tttgacaggt
     3301 gtagattatg tatttgttaa ctacaacaag ccaaaccagg aaaaattgga acatgtgaat
     3361 gttgcccagc tggaagaata tatcaaacaa gatcagtttg caccaggtag catgcttcca
     3421 aaagtagaag cagctatcgc ttttgtcaat ggtcgtccag aaggaaaagc agttattact
     3481 tcccttgaaa atctaggcgc cttgattgaa tctgaaagcg gaacaattat tgaaaaagga
     3541 taagttgttt tactaataag atgtattcta tttctagtat ctttatatca aattagaaat
     3601 tattcttgaa aacatgtaca atatttcaaa agatactagt tttagacttt aatatggtaa
     3661 aacaaatata aatagaaagc gttttcttga atgtttattt aagaaagtag ttggtttttt
     3721 acactttgtt agacatcagg aggaaaaaca aatgagtgaa aaagctaaaa aagggtttaa
     3781 gatgccttca tcttacaccg tattattgat aatcattgct attatggcag tgctaacttg
     3841 gtttatccct gcgggggcct ttatagaagg tatttacgag actcagcctc aaaatccaca
     3901 agggatttgg gatgtcctca tggcaccgat tcgggctatg ctaggtactc atccagagga
     3961 aggttcgctc attaaagaaa cgagcgcagc gattgatgta gccttcttca tccttatggt
     4021 tggtggtttc cttggcattg tcaacaaaac tggtgctctt gacgtaggga ttgcctctat
     4081 cgtgaagaag tataagggcc gcgaaaaaat gttaattttg gtactgatgc ctttgtttgc
     4141 cctcggtggt acaacttatg gtatgggtga agaaacaatg gccttctatc cactccttgt
     4201 gccagttatg atggccgttg gttttgatag cctgactggt gttgcaatta ttttgctcgg
     4261 ttctcaaatc ggctgtttgg catctactct gaatccattt gcgacaggta ttgcttcagc
     4321 gactgcggga gttggtacag gggacggtat cgtacttcgt ctgatcttct gggttacctt
     4381 gactgctctt agtacttggt ttgtttaccg ttatgcggat aagattcaaa aagatccgac
     4441 taagtcactg gtttatagta ctcgcaaaga agatttgaaa cactttaacg tagaagaatc
     4501 ttcatctgta gaatctatac ttagcagcaa acaaaaatca gttctcttct tatttgtgtt
     4561 gacattcatc ttgatggtat tgagcttcat tccatggaca gaccttggcg ttaccatttt
     4621 tgatgacttt aatgcttggt tgactggtct tccagttatt ggtaatattg tcggttcatc
     4681 tacttctgca ctaggtactt ggtacttccc agaaggcgca atgctctttg cctttatggg
     4741 tatcctgatt ggtgttattt atggtcttaa agaagataag attatctctt ccttcatgaa
     4801 tggtgctgct gacttgctca gtgttgcctt gatcgtagcg attgctcgtg gtattcaagt
     4861 tatcatgaac gacggtatga ttaccgatac aatcctcaac tggggtaaag aaggcttgag
     4921 cggtctatct tcacaagtct ttatcgttgt aacttatatc ttctatctac ctatgtcatt
     4981 cttgatccca tcttcatctg gtcttgccag cgcaactatg ggtatcatgg ctccacttgg
     5041 agaatttgta aatgtccgtc ctagcttgat tatcactgct taccaatctg cttcaggtgt
     5101 cttgaacttg attgcaccaa catctggtat tgtgatggga gctcttgcac ttggacgtat
     5161 caacattggt acttggtgga aattcatggg caaactcgta gtcgctatta ttgtagtgac
     5221 catcgccctt cttctccttg gaaccttcct tccattccta taaaatagtg agtgaggtga
     5281 ttccatgaaa atagatataa caaatcaagt taaagatgaa tttcttatat cattaaaaac
     5341 cttgatttcc tatccttcag tactcaatga aggagaaaat ggaacacctt ttggacaagc
     5401 aatacaagat gtcctagaaa aaactttaga gatttgtcga gacataggtt tcactaccta
     5461 tcttgaccct aaaggttatt acggatatgc agaaatcggt cagggagcag agcttctggc
     5521 cattctctgt catttggatg ttgttccatc aggtgatgaa gcagattggc agacaccgcc
     5581 atttgaagca actatcaaag acggctgggt attcggacgt ggtgtccaag atgataaagg
     5641 cccttcgctc gcagctctct atgcagtaaa aagcttgctg gaccaaggta ttcagttcaa
     5701 aaagcgcgta cgctttatct ttggtaccga tgaggaaacc ctctggcgct gcatggcacg
     5761 ctacaatacc atcgaagaac aggccagcat gggctttgca cctgactcat cttttcctct
     5821 gacctatgct gaaaaagggc ttctacaggt caaactttat ggccctggat cggatcaact
     5881 agagcttgaa gtaggaggcg cctttaacgt tgtaccagac aaggccaact accaaggtct
     5941 cctctatgaa caggtttgta acggtctcaa agaagctggt tatgattacc aaaccactga
     6001 acaaaccgta acggttctcg gagtgccaaa gcatgctaag gatgctagtc aaggtatcaa
     6061 tgctgtcatc cgactagcta ccattcttgc tcctctccaa gaacaccctg ctctcagttt
     6121 tcttgcaaca caagcaggtc aagacggcac aggaagacaa atctttggtg atatagcaga
     6181 tgaaccttct ggtcacctat cctttaatgt cgcaggtctc atgatcaatc atgaacgttc
     6241 tgaaatccgt attgacattc ggactcctgt cttagctgac aaggaagaac tagtagagtt
     6301 gcttacaaga tgtgcacaaa actaccaact ccgctacgaa gagtttgact atctagcgcc
     6361 tctatacgtc gcaaaagaca gtaaactcgt tagcacactg atgcaaatct accaagaaaa
     6421 gactggcgat aacagtcctg ctatttcatc cggtggtgcc acttttgctc gcaccatgcc
     6481 aaattgtgta gccttcggcg ccttattccc aggagcgaag cagacagaac atcaggcaaa
     6541 tgaatgtgcc gttctagaag atttgtaccg tgctatggat atttatgccg aagccgtcta
     6601 tcgacttgca acttaatcag gcaactgttt ctacaaaaaa aaatcgaccg attaatgaac
     6661 tgcaccccaa aagttagaca gaataaatct aacttttggg gtgttttatt atgaaattga
     6721 gttatgaaga taaagttcag atctatgaac taagaaagca aggacaaagc ttcaaacagc
     6781 tttcaaaaag atttggtgtg gatgtttctg gtctaaagtc atctgaatct ttgagatgag
     6841 ctttataaat cgcttttttc agtttttgca ctggtatttc gataaactca aactttttag
     6901 ccgttgtatt gcctgatttt atagtatgtt gaaactagaa tagtacacct ctacttctaa
     6961 aacattttta gaaatcaatt tgactgtcct gattgatttg tcctgttctt atttcatttt
     7021 actatatttg agccacttcg tctttaacgg ctttattcat aagctcttgt aatttttctt
     7081 tactatcaat tacttctgat tttccgttgt aatttattgt aataggtttt aacttaccta
     7141 atttctcgac acgctcatta atttgatctt ttttgaaggc tgcatatgtt tttcctaaga
     7201 ttttttcgaa aatatattta tcagatagcg gtttgccttc ttcttcagct tggtttttgt
     7261 attgatttga aacataagga acaaatcctt catagtaacc taatgctccc ataagttcaa
     7321 aagcttgttt tctaattcaa accattgcaa ctcagatttc agcttttcag ataaatcctg
     7381 ctcatccaaa taatgacttg aaattagtgc tgaactcgtt tctgtatcct gtacaggctg
     7441 agcacccata ccagcaaaaa aataaactcg ttcctagcaa gaccgaacaa gctcctattg
     7501 catatttcct caaagaaaaa cgctgctttc tctcaaattg aaattctttc atcccatctc
     7561 ccatcattca ttattactgt atattttgta tatcagaaat agtttgtatt cacaaatctt
     7621 tctagttatt cccttatcat tcctaattaa gggagataac atacaataat ttttagttaa
     7681 atgtatatcg atgttttttg tttttcttaa taaacgcaat acaaaaagag cctgttgcca
     7741 agctctttat actcaatgaa aatcaaagag caaactagga aactagccac aggttgctca
     7801 aaacaccgtt ttgaggtagt agataagact gacgaagtca gctcaaaaca ctgttttgag
     7861 gttgcagata gaactgacga agtcagtaac atctatacgg caagacgacg ctgacgtagt
     7921 ttgaagagat tttcgaagag tattagtcta ttatttcttc tcagcgcgaa gggctgacaa
     7981 gatttgtgta cggatatcat ccacaccatt tggagtattt ggtaaaaaga tggtttgatt
     8041 tcctttagag gcaaaggtat tcaaggtatc caaatactgg ttggtcaaga ggatagacat
     8101 gatttgttct tctgtcatgc caacattggc ttccttgagt tcggtgatag actctgccaa
     8161 tccatccaca atcgccttac gttgttgggc aatccccaca ccatgaaggc ggtctttttc
     8221 tgcttcggct tcagctgcag tgacaatttt aatcttgtca gcttccgcca attcttgtgc
     8281 tgcgacccgc ttacgttgcg ccgcattgat ttcattcata gattgcttaa cttctgcatc
     8341 tggttcgacc ttggtaatca aggttttcac gataatgtag ccgtaagtgg tcatttcttc
     8401 tgctacttgg tgttgaacct caagggcaat ctcatctttt ttctcaaaca attcatccaa
     8461 ggttaatttt ggaacagaag agcgaagagc atcttcgata taagatttaa tctgagattc
     8521 tggacgtatg agtttatagt aagcatctgt cacgctctgc tcgttgacac ggtactgagt
     8581 cgctacattc atcataacga acacattgtc cttggtctta gtctcaacca caatatcact
     8641 ttgcaacaag cgcaactgaa tccgtgctgc aatcgagtca atcccaaaag gcaagcgaat
     8701 atgaataccg ctattagcaa ccttttggta tttcccaaag cgttcaataa tcgccaccga
     8761 ctgctgacga accacataaa ctgtactcag tgtgactatc accaatagga gcacacaaac
     8821 aatcagaaaa atcatgaaaa atattgccat aatggaacct ccacaagtat ttttctagta
     8881 ttatagcaca tttaaagaag gctgtgccgt ttttactgcg atttttcctg aaatgtcaat
     8941 aattagaggt gaattgtcct attgtcgtct aatctcttgc taaaataact ctttataaaa
     9001 ggcaatcgtt tcttctaagg ttggcataaa tggatttcct ggtgcgcagg catcaatcaa
     9061 ggcattctta gaaaggtatt caaagtcgaa atctttttct tcaataccaa gttcagtcaa
     9121 tttcttagga atacctactg tctcagaaag cttctcaatc tcagcaatcg cataatcggc
     9181 acactcttga tctgatttac cttccacatg aaggcccaag gctttggcaa cattgcggaa
     9241 agcttctggt acacgtttag cattttcacg ttctataact ggtagcaaca tggcacagca
     9301 aacaccatgt ggcaaattat atactgcacc gagttggtga gccattgagt gaacataacc
     9361 aagaccagca ttattaaagc tcataccacc aaggaagatg gcattgacca taccttcacg
     9421 cgcttcaata tcatgtccat tttctacagc acgaggaaga tattccttga taagctcaat
     9481 agcaccaata gacagtttct tggttacatc ataagcacca ggtgttacca aagcttcaac
     9541 agcgtgagtc agagcatcca taccagtagc agcagtcagg cctttaggtt tggaaagcat
     9601 gagttccggg tcattaacag agataagagc aaggctattc ttgtcaacca ttaccatctt
     9661 aaccttgcgt tcttcatcag taatcacata gttaatggta atttctgctg atgtacctgc
     9721 cgttgtattg atagccacaa ctggcaagcc ttttttagca gacttatgaa gcccttcata
     9781 atcctgtggt tttccaccat ttgtagccat gatagaaata caactagctg catcctgtgg
     9841 agaaccccca ccaagactga tgataaaatc acagccatgt tcttgcaaag cagctacccc
     9901 atctgtgaca ttcttacaag tagggtttgg ctctacatcg ctaaagatga catattcgat
     9961 tccttctgta tccagtggtt ttaaagtctt aggtaaaata tcactgcctt cgatgtactt
    10021 atctgtaacc aaaagtgcct ttttatagcc aagttccttg atataaggac ccacttcatt
    10081 tacaacacct tttccaataa ggttgactgc cggaacataa aatgtagcca tataattttc
    10141 ctcctgcgcc tccgcgctat ctatttaaca tcattatatt actttttgta ttcgttttta
    10201 attatttgtg aattgtttaa caaatctttt taaaaggata ggagggacgt aagttcacct
    10261 cctatataaa caagttttat ttatgtagtg gtcccaacaa ctgacatgca cgatagtctg
    10321 ctgattctag agcttctgtt ccaaataagg accaattttt aggtctaaag atatcttcct
    10381 caggtacatt atgcatattg acaggaattc tcaacataga agccaaggta atcaagtctg
    10441 ctccaatgtg tccatatgtt atggctccgt gattagctcc ccaattattc atgacgtcat
    10501 agacagactt gaaagcacct tttcctgtca aacgtggagc aaaccaagta gttggccatc
    10561 ctggatctgt acgattatct aaagtatggt gaacatcttc aggaagttca agtgtataac
    10621 cttctgcaat ttgtagcact ggaccaaccc cttttagaag attgagacgt accattgtta
    10681 ctggcatatc ccccttcgtc aagaaacgag ttgagaatcc tcctccacgg aagtattcgc
    10741 ggtttgctgg tgggaagtct gtattttcaa gcatagcctg cacttcactt tcatccaact
    10801 cccagaatgg tttcataaca ggtttgccat ctcgagtagc ttgccctgta ccatccaatg
    10861 tacaagaacc agagttgatt agatgtaaga agccagctgc agcacgaccc tctaaagtat
    10921 gtcccgttac acgtttaaca gcctctggac tccaataagt gcgcacatca gcaaagattt
    10981 gtggagtatt tgttaataga taattaaaga gcatagacac accatttagt gaatcattct
    11041 ctgtcgcaaa tacaaatggt tttcgaatac cattccagtc aaactgagta ttgaggaaag
    11101 tttccataaa gtccccattt gggaaatggt ctgtccactg gcgttgacct tggaaaccag
    11161 ctactaaagc atggtgacca actgcttctt cctcaaaacc aagttcagca agtcttgggt
    11221 taccaaccat taagtcacgt ccaatcatga acatcttaat aacaaattcc cattgttttt
    11281 ctttctcttc tttagaaaga atcaaatcct cacgatttcg gtcaacccct tctttgatat
    11341 gttcttttac ccaaaccata gcacgttcga actcttctgg gtcataaata ccacggtcca
    11401 tacggcgcgt gaactccgtc atatctaccg attcatttcg cattcctaag tattcttgga
    11461 agaaatctgg atttacaata gaaccgccaa tccccatcga aacactaccc attgataggt
    11521 aagcagtgtc tctcatcaag ccagttgcaa gagctgcacg cgcataacgt aaaagttttt
    11581 ctttgacatc ttctggaata tctgtatcat tagcttcctg aacatctcta ccataaatcc
    11641 caaaggctgg aatccctttt tgagcatgtg aagctagtac agctgcaaga tagacagctc
    11701 ctgggcgttc tgtcccatta aatccccaaa tagcatgagg aatatctgga gacatatcca
    11761 tagtttcact accataacac cagcatggtg taactgtaat tgttgcgcaa acatttgatt
    11821 ttttaaacaa ctcatgggaa gctgcagcct ctggaacacg accaatggta gatggagaaa
    11881 tcacgcattc cacaggttcc ccatctggat atttcaatgt gcttgaaatc aaatctgcca
    11941 cacttttagc catgttcatt gtttgcactt caagtgattc gcgtacacct tgacgacgac
    12001 catcaatagt cggacgaatc ccaatacgtg gatgttgaat catactattt tcctcttatt
    12061 tttctgtttc tttcatacta ccgatgataa tttttaaaat tgcttataag tataaacctc
    12121 tacctctgcc aaacttaaag gaattcctgc gtttctcaaa gaaattctaa ccttttttcc
    12181 cttcagccc
//
LOCUS       DAVGJJ010000057        12160 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000057 DAVGJJ010000000
VERSION     DAVGJJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.057"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(169..1371)
                     /gene="rpsA"
                     /locus_tag="VVE35_001439"
     CDS             complement(169..1371)
                     /gene="rpsA"
                     /locus_tag="VVE35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU3061614.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1533..1604)
                     /locus_tag="VVE35_001440"
     tRNA            complement(1533..1604)
                     /locus_tag="VVE35_001440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Gln,seq:ttg)
     gene            complement(1614..1694)
                     /locus_tag="VVE35_001441"
     tRNA            complement(1614..1694)
                     /locus_tag="VVE35_001441"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1658..1660),aa:Tyr,seq:gta)
     gene            complement(1744..1974)
                     /locus_tag="VVE35_001442"
     CDS             complement(1744..1974)
                     /locus_tag="VVE35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU3061615.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2142..2786)
                     /gene="pcp"
                     /locus_tag="VVE35_001443"
     CDS             complement(2142..2786)
                     /gene="pcp"
                     /locus_tag="VVE35_001443"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU3061616.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2801..3724)
                     /locus_tag="VVE35_001444"
     CDS             complement(2801..3724)
                     /locus_tag="VVE35_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU3061617.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3721..4410)
                     /locus_tag="VVE35_001445"
     CDS             complement(3721..4410)
                     /locus_tag="VVE35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU3061618.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4475..5179)
                     /locus_tag="VVE35_001446"
     CDS             complement(4475..5179)
                     /locus_tag="VVE35_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3061619.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5235..6257)
                     /locus_tag="VVE35_001447"
     CDS             complement(5235..6257)
                     /locus_tag="VVE35_001447"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU3061620.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGALYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6389..8860)
                     /gene="parC"
                     /locus_tag="VVE35_001448"
     CDS             complement(6389..8860)
                     /gene="parC"
                     /locus_tag="VVE35_001448"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU3061621.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9280..11223)
                     /gene="parE"
                     /locus_tag="VVE35_001449"
     CDS             complement(9280..11223)
                     /gene="parE"
                     /locus_tag="VVE35_001449"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU3061622.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11359..12000
                     /gene="plsY"
                     /locus_tag="VVE35_001450"
     CDS             11359..12000
                     /gene="plsY"
                     /locus_tag="VVE35_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU3061623.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12045..>12160)
                     /locus_tag="VVE35_001451"
     CDS             complement(12045..>12160)
                     /locus_tag="VVE35_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU3061624.1"
                     /translation="KQLEQAIIDYIDYYNNKRIKVKLKGLIPVQYRTKSFG"
BASE COUNT         3506 a         2753 c         2132 g         3769 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttt atcgctttga acatcaaaaa agaaaggacg
       61 aaatttgtcc tttctcgagc ttagcttttt ttcaacccac tacagttgac aaagagccga
      121 aaaatccctt gtttagtaaa caaaggattt tgtgaatttt caatttgatt aaagttcgat
      181 atcaccaaac aaatcagcca ttgaaaatcc tgtttgtgtt tctggaagtt cgaaatcacg
      241 cttttcttga cgtcttggac gacgtggacg agcagcacgt ttttcttctt tttgtccttc
      301 ttcttgggct ggacgttctt caagagcttt aatagaaagt gatacgcgtt ctgcatctgc
      361 gttaacttca agaactttaa cttgaacttc ttgaccaact ttaagagctt cttttggatt
      421 ttcaatccgt ttgtgtgaaa tttgtgatac gtgaacaagt ccatcgatac ctggcaatac
      481 ttcaacaaat gcaccgaagt cagtcaaacg tttaactgtt ccttctacta catcaccttt
      541 agccaatttt tgctcaacgc catcccatgg tcctggtact gttgctttaa gtgaaagtga
      601 tacacgtcct tcttcttcgt taagatcaag gattttcact tcaatttctt caccaacagt
      661 tacaactgat tttggtgata cgttacgttc atgtgacaat tcagtcaagt gaaccaatcc
      721 gtcaacacca ccaaggtcta tgaaagcgcc gaagcttgtg atacgagcaa ctttaccagt
      781 tacaacatca ccaacagcca atttaccgaa tacttcagcg cgagctgctg cagtagctgc
      841 ttcaacaact tcacgacgtg aaaggatgaa gcggttttct ttagcgttaa cttctttgat
      901 tttagtatca aattcttgac ctacaaaacg ctcagcgtta cgtacgaaac gagtatccaa
      961 cattgaagct gggataaatc cacgaacacc ttcaaattct actgaaagtc cacctttaac
     1021 ggcacgcgtt cctttaacag taacaacttc ttcttcgcga ccaacaagtt tgtcccatgc
     1081 tttgcgagct tcaaggcgtt ttttagatac aaggtatgta actgtatcag tatctttacc
     1141 aactacttga cgaagtacaa gaacatccaa tacttctcct actttaacaa agtcattgat
     1201 atctgcatca cgatcgtttg tcaattcgcg aagagtcaag acaccttcaa caccagttcc
     1261 agagattgca acgttagctt gagtcgcatc aactgtcaat acttcagcac taacaacatc
     1321 accagtctca acttgactaa cgctatttag caaatcttca aattcgttca tctaaaaaat
     1381 cctccaacaa tcaagttttt cttaaacttg acatacttat aatttttttc ctaagcaccc
     1441 gcaaggacat gttcatatca ttcacgtctt tcaattataa aaataaaata ccctaagata
     1501 ttttgctttt tatcttgatt attacctaag aactggggta gctggattcg aaccaacgca
     1561 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccattaatg aaatggagag
     1621 agagggattc gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc
     1681 ttcgctatct ctcctacaat caacatggac tattatatca tgaaaatttc aaaaaaacaa
     1741 gacttatttt gctaaattat aattttcaat caaaatttcc acagcttttt ccaatttttt
     1801 ataaaaacta tcgacattcc cattctttat aatggcaaat tgtccgtcta attcggcaat
     1861 ttctccgata acttttttac cgatagtcaa tgtataacct tcaaaactgt ctttaccaac
     1921 attaactttg gcatccgcta cttgaatttc gattttttta tctttcttgc tcatttcata
     1981 cctctcttca ctttttctat tttacaagaa aatcctaaaa ctagcaagaa aaaactctat
     2041 atcaggcttc tctgatatag agttttatgt aaacaacctc aatcgattta gcatagctaa
     2101 tagttagtaa acaagcataa ttgactatcc tttttaggtt atcagtgagt ctcaccgcct
     2161 actaactgca aatccttatc tttgtagtct acaatagctt caatagctac ctctatgcct
     2221 cttacaatat cacatagact cattcctgca gtattcggct tatttactac ttgttccatc
     2281 atatatggaa tatgcatgaa acctgctctc atattaggga attttttatc cactaaataa
     2341 agagcttggt acatcaaatg attgcaaaca aaggttcctg cactattgga tacaactgcc
     2401 ggaagtccct gttttttgat agcttgtacc atcgctttga taggtaaact actaaaatag
     2461 gccgatgctc catcaatacg aatcggtgta tcaattggtt gattgccttc gttatcaggt
     2521 atgcgagcat catcttgatt aatagccact cgttcaggtg ttaagccggt cctgccgcct
     2581 gcttgtccaa tacaaagtac agcatctggt tgatatcgta atatttctgc ctctaaaact
     2641 tctgacgact tataaaaaac cgttggaatt tctacccagc gaacttcagc cccattaatc
     2701 tcagatggta ataattttac agcctccaaa gctggattaa tcttttcacc tccaaaagga
     2761 ttaaaacctg taaccaatat tttcatttta ttttccttta ctaaaatgcg agaaagtaca
     2821 ttaagaatat gtgaataaca atcattacta gagcaacacc tgcttgagcc tttataacgc
     2881 cattctgatc tttcatatcc atcaatgctg ctggtagagc gttaaaatta gcagccattg
     2941 gggtcaataa ggtcccacaa taacctgctg tcatggcaag agcaccagcc acaattggat
     3001 tagctcccag agcaaataca aagggaactc caacacctgc tgtaataacg gtgaatgctg
     3061 caaaagcatt tcccataatc attgtgaata gaaccattcc aagaacatag gccaaaactc
     3121 ctataaagcg actatctgaa ggaacaatac cgctaatcag atgagagata acatcaccaa
     3181 cacctgctac agtaaaaata gcccccaaag cccctaataa ttgaggaaca ataccacttg
     3241 ttgaaacttg ctgagtcatt cgattatttt ctgataacag actcttaggg tgactattgg
     3301 taatcacaag aacagaaatt gtagcaaaca aggcggcaag gctaatcgaa atcttgctaa
     3361 attctggaat catttgcgct aagaccaacg caagtattgc catcagcata actggaataa
     3421 aaattttatt tttcaacctg ttagattcaa tattggcttt catttcatct aaggatggca
     3481 aggttccgat acggacttgc ttaaacaatg ttaacagcga taataggatt acaataatac
     3541 caatactcat atttggcata taggaaccac ctataaacgt aatagacaat agagtccaaa
     3601 atgcagatgt cccaagtcga actgggtttg ttttatcttt ataactacaa taggctgtat
     3661 ggagaaattg acaaccaatc acaatatagg tcaactctaa tagttgcttt gccaactctg
     3721 tcatttttgt tctcctcccc tagtcttttt tgatatcaat tttttatcaa ataaataatt
     3781 gtaaatcccc actacaataa gtgttataac agcaacaata atagatgtag aagcaatccc
     3841 tgcataattg ctttcatagc ctaactgatc taatgttccc cctatcaaga ggactccccc
     3901 agcacctaca aacgtatttt gagcaaagaa atttccaaaa ttttcattcg cagccgcacg
     3961 cgcttttatt gtctcatctt caacctctgt taactttcta cctaattgag actctgcagc
     4021 tgcttctccc ataggttgaa ccaaaggtct gacaaactga gggtgtcctc ctagacgaat
     4081 tgaaaagaaa ccagctaact ctcgaataaa aaaataaact gtatagaagt ttccaactgt
     4141 cagaccttta atctttcgaa tcaaatcgat tgatcgttgc ttgagtccaa aggtttctga
     4201 cagccccaca agaggcaagg taaccataaa aatcgtgagc actcgctgat tgctaaattc
     4261 ttttcccaaa atctccaaaa attcaacgag agaaacacct gaaactaaag ctgtaaccaa
     4321 accagctaag actactgttg caattgtatc aaattttaaa ataaaaccca caacaatgat
     4381 tgctattcct attaatctaa tccactccat atcaaactcc tttatattca aaatgacagt
     4441 attttaaaaa ttttatcaag atcaatacca ttccttattt aatgtatttt tctagttctt
     4501 tttggtattt gctattggat tccaattttt ctttttgcca ttttttaaaa acctcgttat
     4561 attcttttgt tgtaacaata tctttttgca atttcattcc tttaaagata tatggttccc
     4621 ccttaatacc aacttgtgag tatggttttg agaatggtac tacgttactt acaactggag
     4681 aaccaccaga tgaagctgtt ggcatcaata atgaactatc tgtcgaccaa gcttgagctt
     4741 tggcatattt ttcatatctc ttctctaggt cagtggtctc agaaacagca tcttctaaca
     4801 atttcttata tttatccaaa ccaggtttag ctacaacatc cttatctttt cctttcgtaa
     4861 taccaaggtg tttcatggca gaaccagatt ttggatccat aatattcaag taagacgctg
     4921 gatcttgata gcttggagcc catcctgtac tattcaaatc atagtctttt tgagaaggag
     4981 caacattgcc gtatttatca ttttccatca aaccatcaat aacatttcca ataacgtctg
     5041 tcctcgatgt tcgagtcgct atactgtagc ccaatgatgc tggatctact gcatagacat
     5101 aagaaaatgt tgtcggtgca tctgcttctt tatcagtttt tccacaagcc actaaaatag
     5161 ctgacgtgct caggaccact cctgctgtta agagccactt tttctatttc ataaaaaatc
     5221 tcctttggtt tattttaatc tacttttaca atccaacctt ctggcgcttc aatatcgcca
     5281 aactgaatac ccgtcaattc attatataat ttacgcgtca caggacctac ttctgtttca
     5341 ctatagaata catggaaatc atcaccatgt tgaatacctc caattggaga aataaccgct
     5401 gctgtaccac aggcacctgc ctctacaaaa cggtcaagat tatcaattgg aacatcaccc
     5461 tcaataggag ttaatcccaa gcgatgttct gccaaataaa gcaaggaata cttggtaata
     5521 gatggcaaga tagatggact caatggtgtt acaaattcat tatcagctgt aattccaaag
     5581 aagttagctg atccgacttc ttcaatcttt gtatgagttg atgggtccag atagataaca
     5641 tctgagaaat gacgtgactt ggccattttt cctggtaaga gacttgcagc atagtttcca
     5701 ccaaccttag ccgcacctgt accatttggt gctgcacggt cgtactcatc ctgaatcaag
     5761 aagttggttg ggaccaaacc acctttaaag taatttccaa ctggcatagc aaagatggtg
     5821 aaaatgtact cttctgccgg ttttaccccg ataatatctc cgacaccaat caaaagaggg
     5881 cgaagatata aggctccacc tgttccgtat ggtggtacgt attcttcatt cgcacggaca
     5941 actgctttac aagcttctac aaacatgtct gtcggaactt gtggcatcaa gagacggtca
     6001 catgtacgtt gcagacgttt agcattttca tcaggacgga acagttgaac actgccatcc
     6061 ttagtacgat aagctttcaa accttcaaat gcttgttgtc catagtgaag acttggagaa
     6121 gactctgaaa tatgcaaagt tgcatcctct gtaagctctc cttgatccca ttgtccattt
     6181 ttgaaatgag caagatagcg ataaggtaat ttcatatagg aaaaaccgag gttttcccaa
     6241 tcaatcgtta ctgtcatatt ccactcctta ttctaaaaac ctatttctta tattctacac
     6301 tatttttcta aaatagcaag tatattttgt aattttcaga aaatttctcc aataaaaacc
     6361 agctcttaga actgattctt catttcactt atttatcttc agtaactact tcctgaagat
     6421 aagcgtcaaa aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc
     6481 gttctgacaa gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga
     6541 caagcagagt ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt
     6601 gctctgcaac tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga
     6661 cttgtaatcc tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca
     6721 agcttccacg ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga
     6781 ggacatcatc ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg
     6841 gaacctcttc gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat
     6901 ctagtttaat cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact
     6961 tgacagactt agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga
     7021 tttgaccaag gcgagtcact gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt
     7081 ccacataaag gatttcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc
     7141 cgatgtcctt ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa
     7201 gacttgtgaa catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt
     7261 catcatcacg cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac
     7321 gcttgatgta acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag
     7381 ctgtatcaat ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa
     7441 atttcttctt gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac
     7501 cgataatagc cgccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga
     7561 caaccacatc ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt
     7621 cttccgtaaa atcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag
     7681 cacggataag agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga
     7741 tatggagacg tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc
     7801 gacggtgagc gatatagcta gacaggattg gaacaatccc aacctgacga ggtgtgaaat
     7861 tgtcaatcgc caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt
     7921 agttgagaac aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat
     7981 cacggtcaga ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat
     8041 catcgatttt cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga
     8101 tttgttcctt accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc
     8161 ctttcccagt ctcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag
     8221 ggaagtctgg tccaggcaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa
     8281 tcatgtaaac tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat
     8341 aaccagccga aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga
     8401 ccgttggttc tttctccgta tcgtcaaagt tccatgcaaa aggaactgtc tttttctcga
     8461 tatcctgaag aaggtagcct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg
     8521 caggaggatc tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat
     8581 ttttccagtt ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt
     8641 ggaaattccc catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag
     8701 tattgctatc cttattcata gaataaagaa tacggcgctg aaccggcttc aacccatcac
     8761 gaatatctgg caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc
     8821 ccatgatgtc ctccagggac atgttttgaa tgttagacat aagatacaaa gcccataaaa
     8881 taccaagtga aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt
     8941 cacacagtat ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca
     9001 gtaaaagata ttttacggga attcctcccg tgttcagtta cgataagtaa ccaaactatc
     9061 ctgtttgtat ttttcaatat gaaaatctgg ttttccaaaa ttagtcttag tttgtgtctt
     9121 agccgctccc ttaagcgcct ctttgagata agcactcata gcagattctt cattaataat
     9181 cctgcaattt tttcaaacca agattttcaa actgcttttt cacatagtca ttcacatccg
     9241 actctaattt ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc
     9301 tagcgtaaac ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat
     9361 gagaacattg acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt
     9421 acgtgtttct gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc
     9481 tttgtatcgt tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc
     9541 gtccgtccaa gcgtaggcca cttcttcttt cttgccttta cctttggaca tcttataaag
     9601 aggtgggagg gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa
     9661 aaatgtcaag agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat
     9721 gatcttatca tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt
     9781 ataaatcatg gtattgatct cttcattttt gaggatatcc gccatcttgg ccttggctgt
     9841 attgacaacc ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt
     9901 ggcagaacca ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt
     9961 agattgggct ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc
    10021 atttcggctc tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc
    10081 cttgcggatg aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc
    10141 agccactatt ccatccacaa ctgggcgagc taaggggctt cctagtttat ccttggtctg
    10201 tccttcaaac tgcaagtgtt cttcaggaac taagatagaa agaacggccg ctagtccctc
    10261 acgatagtct gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc
    10321 atagtcattc atgaccttgg taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc
    10381 cttggtgcga acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg
    10441 gagggctact tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa
    10501 gatttcctta tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa
    10561 ctcaatcgct tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa
    10621 ggctgattca ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaagat
    10681 agtcgcgtca ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat
    10741 tttcttcaaa gtcgtgacag gttttccacc attttcgaaa cgttgcttgt aaactgcgcc
    10801 atcacgggta atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac
    10861 tccgtgaagt ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag
    10921 aatggtaaag ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc
    10981 acgtccatgg tcttgaaccg ttagactacc gtctttattg atagttacat cgatacgatc
    11041 accaaaccca gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg
    11101 aagaccagcg ccatcggtcg atccaatata catccccgga cgttttcgga ccgcatccaa
    11161 cccttctagc acctgaatag catcatcatt ataattgtta atattgattt ccttttttga
    11221 cacaaggaac ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca
    11281 aaatttttct ttctccgatg tgacaatttc agcagagatt ctctgctttt ctttcccaat
    11341 tcatgatata ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt
    11401 tcgattccat ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat
    11461 ggttctggta acactggaac gaccaatacc ttccgcattt taggtaagaa agctggtatg
    11521 gcaacctttg tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt
    11581 catctacaag gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc
    11641 cctatctttg caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtaattttc
    11701 ggatttgcgc ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt
    11761 ggcagtatga tttcactgtc tagtgtcaca gcatcgatcg cggctgttat cggggttctg
    11821 ctctttccac tttttggttt tatcctgagt aactatgact ctctcttcat cgctattatc
    11881 ttagcacttg ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat
    11941 aaaactgaaa atttggtccc ttggggattg aacctaaccc atcaagatcc taaaaaataa
    12001 aatgccagtt ctgtgaactg caccccaaaa gttagacaat taatttatcc gaaggattta
    12061 gttctgtatt gcacagggat aagtcctttt agttttacct taattcgttt attgttgtag
    12121 taatcaatat agtctataat agcttgttcc aattgcttaa
//
LOCUS       DAVGJJ010000058        12156 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000058 DAVGJJ010000000
VERSION     DAVGJJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.058"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            394..2409
                     /locus_tag="VVE35_001452"
     CDS             394..2409
                     /locus_tag="VVE35_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3061625.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYSNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2411..3043
                     /locus_tag="VVE35_001453"
     CDS             2411..3043
                     /locus_tag="VVE35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061626.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLTG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            complement(3971..4384)
                     /gene="ndk"
                     /locus_tag="VVE35_001454"
     CDS             complement(3971..4384)
                     /gene="ndk"
                     /locus_tag="VVE35_001454"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU3061627.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4495..8172)
                     /gene="rpoC"
                     /locus_tag="VVE35_001455"
     CDS             complement(4495..8172)
                     /gene="rpoC"
                     /locus_tag="VVE35_001455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU3061628.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8205..11816)
                     /gene="rpoB"
                     /locus_tag="VVE35_001456"
     CDS             complement(8205..11816)
                     /gene="rpoB"
                     /locus_tag="VVE35_001456"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU3061629.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
BASE COUNT         3417 a         2604 c         2533 g         3602 t
ORIGIN      
        1 aaaattacat aaaaatgctt ggctaaataa aatatatctg ttaaaataag gataatgaaa
       61 aagaaaaggg tttcacgaat gaagaaaaat cgtggaattc aaaaattagc tatattagta
      121 ttactaggtg tttttatgtt tagtaataca attccttacc aacagtttat tcagaagaat
      181 agacaattgg agattcgagt gcaatcgcaa aagaagtcca atggtcttga tgttgggaag
      241 gctgattgat ttcgtattac tttaaaattc aactatttag agaaaggtat tctgatgaga
      301 gcttgtaaca ataccagata gatattagta agcttgttag tttatagagg gcaggggtat
      361 ttctgccctc tgttttagaa taaggaatgc actatgaaaa aattatttat actcatatcc
      421 aacctgcttg ctagtttgtt ctttgtttgg gtatttacca tttggactga tacatatgtc
      481 tcctattatt actccaacgt tgttgtacat gattcttccc ctgaaacaac ttttcagcat
      541 gttgctacac gcttggaaaa actagcagaa gaaacagata gctttattgc gattcaacac
      601 caagatccta actcagaagg tactacagtt ttttcttata cgacctttgg ggacggaaag
      661 ttgcctgatg ggcttcagga aaaaaaacta gaagatgctc aaagtagtag tgttgaaaca
      721 aactattttg tattcgatgg acacctagat attcacttgc taagggagga gctaagtcaa
      781 cttggcttga ctaacatgaa cctgacaatt ccatctaaac tatctacgtt aatggctatc
      841 tttagtaacg gatttcagtt aatcagtcta ttgattttca tcttaacctt tgtagcactg
      901 actttaatta gtcaaattag ccaattacgg tcgtcgggca ttcgcctcat ttcaggagag
      961 aaacggtggt ccattttcct tagaccagtt ggtgaggatt tgaaagggat agctgttggt
     1021 ttcagtttag ctggcgtgct cgccattctt atgcaaaaaa ttctgtcgct tccaacgcaa
     1081 tccctaatga ccataggagc aggcttgctc agttataatc tcatcttgtt gtcgatctcc
     1141 ctgtttttcg ctcaactctt cgcagttggg ataaaaaaga tacacctgat gcagattata
     1201 aaagggcaag tgcctgtcag aggaatcatt agtttgattt taatcggtca actattagcg
     1261 attattattg taacgctggg aatagggagt agcttaaagt attcccaagc ctggcaacag
     1321 catcggattg gacaagaggc ttggagtcag gaaaggcaac tgattaccct atcaatcagc
     1381 cgtgaaggaa cgagtcctgg ttttgatgaa caagctcaaa ggaaactcag aacttggtat
     1441 caattgatgg atctggctgt ttcagaacaa aaggctttct tatctagaca ccagttaatt
     1501 gaccgtactt tgcaaaatgg catggcttcc tccaaaaact tgataacctc tacagaatgg
     1561 cacgattaca acccgaatgg caatgtcctt atcgtcacac cgcaatactt ggagcgtcaa
     1621 aacattcctg tagatacaac tattgagcaa aagatgaatc accttgatgt aggggagttt
     1681 gtcttattgc tgcctgaaca cctccgttca gaggaagaac attataaatc tgtttttgaa
     1741 gacgacttaa ccagtcgcat gtctagtcaa gatgaacgac agcaaatgac tgctacggta
     1801 ggttatttag aatcaggtca ggatcgtttt gtgtataata cgacccctat ttcttaccag
     1861 cagtttttga aagatccaat catcattgtt ataacacccc aatcaactgg tccacagtcc
     1921 attttgtttt ggatagacgc agtacagaac tacgttctct ttaatcaatt gtctgatgcc
     1981 caggagctta tccagagaca aggcattgaa aattgggtct cagaaatgca aacaggttac
     2041 cacaactaca tcacattatt ggataatatc cagagagaac gttgggtaat gctagcagga
     2101 gctgtgcttg ggattgcaac ttcaatcttg ttgtttaaca ctatgaatag gctctacttt
     2161 gaagaattta gacgtgccat ttttatcaaa cgcattgcag gtctcaggtt cttagaaatc
     2221 catcgcactt atctctttgc tcaactgggt gtgtttttac tgggatttgt tgcgagtgta
     2281 tttcttcagg tagagatagg agttgctttc ttagtcttgt tactctttac tggtctatct
     2341 cttttacagt tacatgtcca aatgcagaaa gaaaacaaga tgtccattct tgttttgaag
     2401 ggaggttaat atgattgaac ttaaacaggt gagtaaatct tttggagaac gagagttatt
     2461 ttcgaatctt tcaatgacat ttgaggctgg aaaagtctat gccttaattg gttcaagtgg
     2521 tagcggaaaa acaaccttga tgaacatgat tgggaaatta gaaccttatg atgggacgat
     2581 tttttaccga ggtaaagact tggccaatta taaatcgagt gattttttcc gtcacgaatt
     2641 gggttacctc ttccagaact ttggcttaat tgaaaaccaa agtattgaag aaaaccttaa
     2701 gctaggtctc actggtcaaa agttgagtcg gtcggaacag cggttgaggc agaagcaggc
     2761 tttagaacag gtcggcctgg cttatcttga cctagataag cgcatctttg agttatcggg
     2821 cggagaatcg caacgggttg ccttggcaaa agttatctta aagaatccac cctttattct
     2881 ggcagatgag ccaacagctt caatagaccc agcaacctct cagttgatta tggagatttt
     2941 gctatctctt cgagatgata ataggctaat cattatcgca acacataatc cagcaatttg
     3001 ggagatggct gatgaagtgt tcacgatgga tcgtctgaaa taaaaatcct tgtttttaat
     3061 tgcacgatga gttactgaaa tattatcatg aatcaataat tggagttaat ttagaattgt
     3121 actttattaa tattgaggta actttttctt gataaaggaa gaaatagtgg agaggaagtt
     3181 agaatgaaaa aattcgacaa ttatattatt gagaagcctt gcgattctaa ttcagataaa
     3241 ctgcaaaaaa tcttaataat tgaaagtttg gtagatgata ttttgcaatt ttctctcaga
     3301 atcaataata gtgtaggaga gattttcctc ctacaaccgt tttaaaagaa aactatcttt
     3361 attccatgtt attttgagga agatattgtg aaagtcaaag atgatgataa agttgagtgg
     3421 aatttgttag aatttcaaaa atttagagca tttttggctt agtaatctgt gttgaaggct
     3481 caaaacctat ggtaaaaaag tagctttgaa aacgtattgc ctccaaagat ttagttaaat
     3541 aatgatttaa cacaaaaaga aattattgaa gttctggaaa gatgttgttt cagtattgag
     3601 aaaaggtggg aaaaacttgc gattttcaca gagaaaggaa gaaaaagtat agaaatatag
     3661 tcaattgaaa caagaacagg acaaaagaac cttttgtgcc atatttttct cctttcgctt
     3721 tacaattggc ttgaacacct ttattgtatc gcgtttggag tttttttggt ataaccttcg
     3781 acgcgcaccc gcatagcggg tgtttttttg tctcgcacct aacggagcga gacgaactaa
     3841 tagtcactta atcaaaaaac gcaccatatc aaaaactaaa aagtttgata tcatgcgtca
     3901 tgtcttaaac taattgacta tactttctat tcaaatgagc ttttaaccaa ttaattgagc
     3961 caatccactc ttaaaaccaa agagcaattt ctcgcttagc tgactcttct gaatctgaac
     4021 catgtacaac attttggata acctcatttt ctccagcagc ttttgcaaag tcacctcgaa
     4081 tagtgcctgg taaagcttct tctggacgag ttgcacccat catggtccgc caagtttcga
     4141 ttactttggg accagaaatg acacccacaa gaactggacc tgaagtcatg aattcacgaa
     4201 tcggtaggta aaaactctga ccaaccaagt cctgatagtg ctggtcaatc aactcttctg
     4261 aaacctgtga acgaaactcc aatttttcga ttgtaaatcc acgttgttcg atgcgcttta
     4321 acacttcacc cactagccct ctttttacac catctggttt gatgataaag aatgtttgtt
     4381 ccatacccgt ctcctttgtc agcttctttc ttttatttta ccacatttcg tggaaaaatg
     4441 gagaaagttt tcagaagaga gaatgagaga acccgagggt tctctcattc tctcttattc
     4501 tactgtttct tccacagttt caacggcagt atccacaact acttctgttg tttcttcatt
     4561 tccttcttcc tctactggag gattaaggta ttcttcttcg ttgacagcat gtggttcaag
     4621 gttacggtaa cgggccatac cagtaccagc tgggatgatc ttaccgatga taacattttc
     4681 tttaagtcca aggagatggt ctttcttacc acggatagct gcgtcagtaa ggacacgagt
     4741 tgtttcttgg aaggaagctg ctgacaagaa actgtttgtt tcaagtgagg ctttggtaat
     4801 tcccataagg actgggcgac ctgtcgctgg aactccacct gcgataagga catctttgtt
     4861 ggcatctgta aagtcattga tatccatgag ggtacccatg agaagatctg tatcacctgg
     4921 atccatgaca cggactttac ggatcatttg acgaaccatt acctcgatgt gtttgtcacc
     4981 gatttctacc ccttggctac ggtaaacttt ttgtacttca ccgagaaggt acgtttcaac
     5041 tgacaagaca tcacgaactg caaggagacg ttttggttgg atagaacctt ctgtcagagc
     5101 agcaccacgc gctacttggc ccccaacttc gacacgcata cgagctgtaa atggaacgac
     5161 atattcacct tcgccagttt cacccttaac aaagactttc ttggtacgag ttgatgcatc
     5221 ttcttcgata gcagtaactt gtcctttaac ctctgtgata accgcttccc ctttaggatt
     5281 acgggcttca aagatttctt ggacacgagg aagaccctga gtgatatcgg tatttgaggc
     5341 aaccccacct gtgtggaagg tacgcattgt aagctgtgta ccaggttccc cgatagattg
     5401 ggcagcaatt gtaccaactg cttcaccaac ttcaaccgca tcaccagtcg ccaagtttat
     5461 accgtaacag tgacggcaga caccgtgacg agtgttacat gtaaatacag aacggatagt
     5521 cacttcttcc acaccagcat tgacaatttc acgcgccttg tcttctgtaa tcaattcatt
     5581 tggaccaata atcactgcac cagtttctgg atgtttaaca gttttcttag tgtaacgacc
     5641 gttgagacgc tcttcgagag actcgatcat ctcttttcct tctgcgatag aacggatcaa
     5701 gagaccacgg tcagttccac agtcgtcctc acggatgata acgtcttggg caacgtcgac
     5761 caaacgacga gtcaagtaac ctgagtcggc tgtcttaagg gccgtatcgg tcataccttt
     5821 acgagcaccg tgagttgaga agaacatttc caataccgac aaaccttcgc ggaagtttga
     5881 aaggattggc aattccatga tacgtccatt cggagcagcc atcagaccac gcataccggc
     5941 aagctgtgag aagtttgaga tgttaccacg ggctccagag tccatcatca taacgattgg
     6001 gttcttagga tcttggttag caatcaagcg tttctcaagt ttttcacggg cagcacgcca
     6061 ttcagctgta acagcattgt aacgctcgtc gtctgtgatc ataccacgac ggaattgttt
     6121 ggtgatttgc tcgacacgtt tgtgtgattc ttcaatgatt tcagccttgt catcaacgac
     6181 tgggatatcg gcaataccca ctgtcaatcc tgcaagagtt gagtggtggt aaccgagatt
     6241 cttcatgcgg tcaagtaggg cagaagtttc tgtcgtacgg aaacgtttga agatttcagc
     6301 gatgatattt ccgaggtttt tcttcttgaa tggagggttg agctcaagat tgctgatagc
     6361 ttccttgata tctccaccaa gtggcaagaa gtatttagct ggaacacctt ctgtcaagtt
     6421 ggcattgttt ggttcttgca agtatggtag cccctctggc atgatatcgt tgaagagaat
     6481 tttaccaact gttgtaagca agaccttatg tctttgctct tctgtccaag gcttgttgag
     6541 gctgtctgtt gcgataccaa cacgtgagtg gaggtgaaca taaccattgc ggtaagccat
     6601 aaccgcttcg tcacggtctt tgaagaccat tccttcacct tcgcgaccag cttcttccat
     6661 ggtcaagtag tagttaccca aaaccatgtc ctgagatgga gtaactaccg gtttcccatc
     6721 tttcggattc aagatgtgct cagcagctag catgaggata cgagcttctg cttgtgcttc
     6781 ttctgaaagt ggtacgtgga tggccatttg gtccccgtca aagtcagcat tgtaggcttc
     6841 acagacaagt gggtgcaagc gaagagcctt accatcaatc aagactggct cgaaggcttg
     6901 gatacccaaa cggtgaaggg tcggtgcgcg gttcaaaagc actgggtgtt ctttaatcac
     6961 ttcttcaagg atatcccaga tacgctcatc tccgcgttcc accaagcgtt tagctgcttt
     7021 gacgttttgc acgatatcac gggcaacgat ttcacgcatg acaaatggtt taaagagttc
     7081 aatcgccatt tcacgtggca caccacattg gtacatctta agagttggac caacggcgat
     7141 aacggaacgt cctgagaagt caacacgttt accgagcaag ttttgacgga agcgtccttg
     7201 tttaccttta agcatgtggc tcaatgattt caatggacgg ctacctggtc ctgtgattgg
     7261 acgaccacga cgaccattgt caatcaaagc gtcaactgct tcttgaagca tacgcttctc
     7321 attttgaacg atgatacctg gtgcatttaa ctcaagcaaa cgagccaaac ggttgttacg
     7381 gttgataaca cggcggtaaa ggtcattcaa gtcagatgag gcaaaacggc caccatccaa
     7441 ctgcaacatt ggacgaagat ctggtgggat aaccggaagg atgttaagaa tcatccattc
     7501 aggtttgttt ccagacttgt aaaaggcatc caaaacatcc aaacgacgga tggctttgac
     7561 acgcttttgt ccagtagctg ttttcaattc ttctttgagt tcagcaattt ctttttcaag
     7621 atctacttgc ttcaaaaggt cttggatggc ttccgcaccc atcttggcaa caaatgaacc
     7681 ataaccatat tcacgcaagc gctctcggta ttcgcgctct gtcatgatag acttgtgctc
     7741 aagtggtgta tccttaggat caatcaccac ataagccgca aagtagataa cttcctcgag
     7801 ggcacgaggg ctcatatcaa gggtcaagcc catacggctt ggaatcccct tgaagtacca
     7861 gatgtgagat acaggagctt tcaattcgat atgtcccata cgctcacgac gaactttcgt
     7921 acgcgttact tcaaccccac agcggtcaca aacaattcct ctgtaacgaa tgcgtttgta
     7981 cttaccacaa gcacattccc agtcttttgt aggaccaaag atcacttcat caaagagtcc
     8041 ttcacgttct ggtttcaagg tacgataatt gattgtttca ggttttttga cttctccata
     8101 agaccatgaa cggactttac ttggagaagc tagggtgatt tgcatacttt taaaacgatt
     8161 tacatcaacc actatttctt ccctttctat tctaagtgaa ctgcttattc ctgttcagca
     8221 gcttcttctg ttgcttccgc ttttgttgct ttctcagctt cttcagcttc aaaggctgct
     8281 ttagcctctt gggctgcttt ttcgcgggct ttttcaaggt catctacgtg gatgacatct
     8341 tcgtccattc cttcatccaa gtcgcgaagt tccacttctt ggtcatcttc gtctaggaca
     8401 cgcatgtcaa gaccaagaga ttgcaattct ttgacaagaa ctcggaagga ttctggaaca
     8461 cctggttttg gaattggttt gccttttgta atagcttcat aggctttcaa acgtccgttg
     8521 atatcgtcag acttgtaagt caagatttct tgaaggacat ttgacgcacc gtaggcttca
     8581 agagcccaaa cctccatctc accgaaacgt tgtccaccaa actgagcttt acctccgagt
     8641 ggttgttggg taacagttga gtaaggtccg actgaacgcg cgtgcaattt atcgtcaacc
     8701 atgtggtgga gtttgatcat gtacatgact ccaacagaaa cacggttatc aaacggttca
     8761 cctgtacgtc catcgtaaag gattgtcttg gcatcgctat ccatacctgc ttctttaaca
     8821 gttgaccaaa gatcttcaga acttgctcca tcaaagactg gtgtcgcaat gtgaatacca
     8881 agagtacgag ctgccatacc aaggtgaagc tccataacct gaccgatatt catacgtgat
     8941 ggcaccccaa gtgggttcaa catgatgtcg actggagttc cgtctggaag gtaaggcatg
     9001 tcttctacag gaacgatacg agagacaacc cctttgtttc cgtgacgtcc ggccatttta
     9061 tctccgacct taatcttacg tttttgagcg atgtaaacac gaaccaacat gttaacacct
     9121 gattgcaact catctccatt tacacgtgta aagatcttaa catcacgaac gacaccatcg
     9181 gcaccgtgtg gtacacgaag agaagtatca cgcacttcac gagacttgtc tccaaagata
     9241 gcgtgcaaga gacgttcttc agctgaaaga tctttctcac ccttaggtgt tactttacct
     9301 acaagaatat caccttcttt aacctcagca ccaatacgga taatccccat ttcgtcaagg
     9361 tctttgaggg catcttcacc aacgtttgga atttcgcgag tgatttcttc aggcccaagc
     9421 tttgtatcgc gcgtttctga ttcgtattct tcaaggtgaa cagatgtgta gacatcgtcc
     9481 ttcaccaagc gttcgctcat gataacggca tcctcgaagt tgtaaccttc ccaagtcatg
     9541 taggcaacga ttgggttttg tccaagcgcc atttctccat tttccataga aggtccgtca
     9601 gcgatgaaat cgcctttttc aacgacatca ccaactttta cgagagtgcg ttggttgtaa
     9661 gcagtacctg agtttgaacg acggaatttt tggatgtggt aaacatccaa tgaaccatct
     9721 tcacgacgaa cttctacctt gtcagcatct gcgtaagtaa ctttaccatc atactgagca
     9781 atcacagccg caccagaatc gtgggctgct tggtattcca taccagtacc aacgtaaggt
     9841 gcctgaggat taatcaatgg cacagcctga cgttgcatat tggctcccat gagggcacgg
     9901 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacctgtttt
     9961 ggtgaaacgt ccatgtagtc aacaatatta gctggatact cttggttgac cccttggtga
    10021 cgtcccatga caatcttctc agcaaaggtt ccatcttcat tcagacgaga gttagcctga
    10081 gctacagtat attcatcttc ttcatcagct gtcaaccaaa caatttcgtt cgtgacaaca
    10141 cctgtttcac ggtcaacctt acggtatggt gtttgaacaa aaccatattt gttcaagtgt
    10201 ccgtaagatg acaagttatt gatcaaaccg atgttaggtc cttcaggtgt ctcgattgga
    10261 cacatacgac catagtgagt gtagtgcacg tcacgtactt catatccagc acggtcacga
    10321 gtcaaaccac caggtcctaa ggctgacaaa cggcgtttgt gagacaactc agaaagcggg
    10381 ttgtgttggt ccatgaactg tgacaactgt gatgaaccaa agaattcttt aactgcagct
    10441 gttacaggac ggatattgat aatttgttgt ggtgtcaaga cttcattgtc ctgaacagac
    10501 atacgttcac ggacattacg ttccatacga gaaagtccca aacgtacttg gttggcaagc
    10561 aattcaccaa ccgcacggat acgacgattt ccaaggtggt cgatatcatc tacacggcca
    10621 agtccttcag ccaagttgag gaagtagctc atctcagcaa ggatatctgc aggagtcacc
    10681 gtacgaacct tgtcatctgg gttagcatta ccaatgatcg ttacgacgcg atctggatca
    10741 gttggagcaa taaccttgaa tttttgaaga acaacaggct cagtcacaac ggctgcatcg
    10801 tttgggatgt agacaatctt gttcaagtcg ccatccaaat ggctttcaat gctttcaatc
    10861 acgctacgag tcataatcgt accagcttct accaagattt ctccagtttc agggtctacc
    10921 aatggctctg caatggtttg gttgagcaaa cgtgttttaa cattgagttt tttattgatt
    10981 ttgtaacgac caactgctgc caagtcatag cgacgtgggt caaagaagcg agccacaagc
    11041 aagctacgtg agctttcagc tgtcttaggc tcacctggac gaaggcgttc gtaaatttct
    11101 ttcaaggctt cgtctgtacg agagtccatt ggattcttgt ggatatcttt ttcaacagtg
    11161 ttgcgaacca attcgctgtc accaaagata tcaaagattt catcatcacc tgagaaacca
    11221 agagcacgaa ccaaggttgt aaatggaatc ttacgagtac ggtcgatacg agtgtaggtg
    11281 atatcttttg agtcgctttc aagttccaac caagctccac ggttagggat aacagttgaa
    11341 ccatagccca ccttaccatt tttgtctact ttgtcgttaa agtaaacacc tggtgagcgg
    11401 accaactgag aaacgataat acgttcacca ccattgatga tgaaagtacc catttctgtc
    11461 atgattggga aatcaccaaa gaaaacttct tgggtcttga tttcgcctgt ttctttattg
    11521 atcaagcgga aggttacaaa aattggtgct gagtagctag catcgtggat acgagcttct
    11581 tctagcgtgt attttggttc cttgatttca tatccaacaa attccaactc cattgtgtct
    11641 gtgaagtttg aaattggcaa tacatcttca aacacttcct taagaccgtg gtctaggaaa
    11701 gctttgaatg agtcagtttg aatttcaatc aaatttggta agtcaagaac ttctttgatt
    11761 cttgaaaaac tacgacgggt acgatgtttc ccgtattgaa cgtcatgtcc tgccaagatg
    11821 attctccttt gtaaataagt tccaagcctt gtcaatcagg cttttctaat cgccatatgg
    11881 ttgtaaaccc cttatcaccg tgtcctcttg acgaattttc agaatcttta agcctctgtt
    11941 acaaatgctc aaaatcttga aaaaaagcac aaaaagagca gctaaatctg actttttcag
    12001 aagatttaac tgctgtgagc cttgtctgga caatatttca gacaaaacct acgacaaatg
    12061 attacccata ttatacccta tttagctaga tttttcaaga ggattcagta ggtttttggt
    12121 aaattttttc ccatagaaaa cttggcatca cattcg
//
LOCUS       DAVGJJ010000059        12084 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000059 DAVGJJ010000000
VERSION     DAVGJJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.059"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            22..177
                     /locus_tag="VVE35_001457"
                     /pseudo
     CDS             22..177
                     /locus_tag="VVE35_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            377..3652
                     /gene="rexB"
                     /locus_tag="VVE35_001458"
     CDS             377..3652
                     /gene="rexB"
                     /locus_tag="VVE35_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU3061630.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMIFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKVSRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDAHSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGSIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3649..7299
                     /gene="addA"
                     /locus_tag="VVE35_001459"
     CDS             3649..7299
                     /gene="addA"
                     /locus_tag="VVE35_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU3061631.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFVGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQFTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7514..8692
                     /locus_tag="VVE35_001460"
     CDS             7514..8692
                     /locus_tag="VVE35_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061632.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8901..>12084
                     /locus_tag="VVE35_001461"
     CDS             8901..>12084
                     /locus_tag="VVE35_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU3061633.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIQEEIQE
                     NPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTKKT
                     QVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSEAVVTDKGEP
                     AVQPELPEAVVSDKGEPAVQPELPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAPLP
                     EYTGPQASAIVEPEQVAPLPEYTGVQAGAIVEPAVQPALPEAVVSDKGVPEVQPELPE
                     AVVSDKGVPEVQPELPEAVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKLQEEEPEK
                     TIELRSVSDVELYSYEANQSKQHTILDKVPDNKSAYFIKVKSAKFKDVLLPVSNIEDT
                     TKDGKVVYKITARLEHLKQSVDNQYQDNFTYYLPKVVQNDGPIYTSFKKLVTDMNSRP
                     DGTFILGATMNAREFELGENQESYVTKNFTGTLIGSHQGKHYAIYNLKKPLFNTLNNA
                     AIQDLTLKDVNISGKNHVASVAMEATNSSTLDNVHANGIIAGELGIGGLVQKVDNSTV
                     RNSSFTGRITNTFVTTSQYAIGGLVGQLTGANARVAHSISTVVISSNGEHSNQTIGGI
                     VGIVENNATVENSYAEGEINNAKPFARVGGVAGSLWTQEGGTDNNTGRLTNVLSDMNI
                     TNGNAISGYHFNGIKETNVYSNKENKVVNVQAVGDEVMSVDSTVERGTEIEKTSVQAK
                     KDEITKKHTITVEDFTNSVKQDVDYTKAAHY"
BASE COUNT         3924 a         2395 c         2544 g         3221 t
ORIGIN      
        1 tagttcaatc attctatcaa gaatattgaa tatataaagg cacgtaatat taaaacttct
       61 tctgatatcg aattagagtc cgatgttgat ggcgataaat cagacaatct acccgtcgac
      121 attaagatac tgggacaaca cattgaagtg tattccgtac ctaagaaaca atcataaaac
      181 tcaaagcctt gttaccgagg cttttttatt atgtgatttt cagtagccag acttattcta
      241 tctaatacaa gtaaagctaa gtatttaaga tttagcagat gatgaagaaa agaaaggatg
      301 gcctaaatat tttaacagat ttacgaggct aatcatggta taatataggg tgatgaaaaa
      361 ttccaacgag gctgagatga aattacttta tactgatatt cgaacttctt tgacagaaat
      421 tctaacaaga gaggcagaag agctagttgc agctggcaag cgggtcttct acattgcccc
      481 caactctctt tcttttgaaa aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc
      541 tttttcgatt accgtcacgc gctttgctca aatggctcgt tatctggtct tgaatgattt
      601 accagctaaa actactcttg atgatatcgg tcttgggttg gccttttaca aatgccttgc
      661 cgaactcgat cccaaggact tgcgtgttta tggcgctatt aagcaggatc ctcaattgat
      721 ccagcagtta attgagcttt accatgagat gaccaaatct cagatgattt ttttggactt
      781 ggagaattta acagatgagg ataagagggc ggatttactc ttgatttttg agaaagtaac
      841 agcctatctt aatcaagggc agttagccca gggaagtcag ttgtcccatt tgattgaggc
      901 tattgagaat gacaaggtaa gtagtgattt taatcaaatc gccttggtca ttgacggctt
      961 tactcgtttt tctgctgagg aagagcgggt tgtggactta cttcacggca aaggtgctga
     1021 gattgttatc ggggcttatg ctagtaagaa agcctatacc agtcctttta gcgagggcaa
     1081 tctctaccaa gccagcgtaa aatttctcca tcatctggct tctaaatacc aaacgcccgc
     1141 tcaggactgt tctcaaactc atgagaagat ggatagtttt gacaaggtct ctcgtttgtt
     1201 ggagtcttct tatgactttt cagaactcgc tttggatgtc gatgagaaag accgtgaaaa
     1261 tttacaaatc tggtcttgtt tgacgcaaaa ggaggagttg gagctagtag cccgtagcat
     1321 tcgtcagaaa ttacatgaga actcagacct gagctacaag cattttcgta ttctcttggg
     1381 ggatgtagct tcttaccagt tatctctcaa aaccattttt gaccagtatc agattccttt
     1441 ttatcttggt agaagcgaag ccatggctca tcatcccttg actcagtttg tcgagtctat
     1501 tttagcttta aaacgttacc gttttcgtca ggaggatttg attaatcttc ttagaactga
     1561 cttgtatact gacctcagtc agtctgatat tgatgctttt gagcaatata tccgctatct
     1621 tggtattaat ggcttgccag cctttcagca aaccttcacc aaatcccacc atggaaaatt
     1681 taatcttgag cgtttgaatg tcctccgcct gagaatttta gcacctcttg aaaccctctt
     1741 tgccagccga aaacaaaagg ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga
     1801 aggagctgtg accaagcagt tacaagattt gacaaccact ttggaagctg tagaacagga
     1861 aagacaagcc gaagtttgga aggctttctg ccatgtttta gaacaatttg cgactgtttt
     1921 tgctggttca caggttagtc tggaagactt cctagccttg ctccattctg gaatgagttt
     1981 gtcccaatac cgtaccattc cagcaacagt ggacactgtt ctggtgcaga gttacgattt
     2041 gattgcacca ttgactgctg actttgtcta tgctattgga ctaactcagg acaatttacc
     2101 aaaaatttct caaaacacca gtcttctgac agatgaagaa aggcaaaacc taaaccaagc
     2161 gaccgaagaa ggcgttcaat tactgattgc cagcagtgaa aatctcaaga aaaatcgcta
     2221 cactatgctt tccttggtca attctgctcg taagcagttg ttcttgtcgg ctccaagcct
     2281 ttttaacgaa agtgaaagta aggaatctgc ctatcttcaa gagttgatcc attttggatt
     2341 taggcggaga gagaagagga tgaatcacaa aggactgtct aaggaggata tggggtccta
     2401 tcacagtctt ttgtctagtc tggttgccta tcaccagcag ggtgagatga gcgatactga
     2461 gcaagatttg acttttgtca aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca
     2521 aggtctggaa aatccagcca tcccaaccag tccaagcagc aagaccttag ccaaggacac
     2581 cttgcaagct ctctatcctg ccaaacagga gttttacctg tctacgtcgg gtttgacaga
     2641 gttttatcgc aatgaataca gttatttcct acgctacgtt ttaggcttgc aggaggaatt
     2701 acgtttgcgt cctgatgccc atagtcacgg gaatttcttg catcgtatct ttgaacgcgc
     2761 cttacagttg cctaatgaag attcctttga ccaacgtcta gaacaagcta ttcaagaaac
     2821 cagtcaagaa cgcgaatttg aagctattta tcaagaaagt ttggaagccc agtttaccaa
     2881 ggaagttttg cttgatgttg cacggacgac tggacatatt ctccgacaca atccagccat
     2941 cgaaaccatc aaagaagaag caaattttgg tggaaaagac caagccttta ttcaattaga
     3001 caatggacgc agtgtctttg tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa
     3061 tggatcgata ggagtagtag actacaaatc cagtctgact cagttccagt ttcctcattt
     3121 ctttaatggg ctcaattctc agttaccaac ctatcttgct gccctaaaaa gagaagggga
     3181 gcagaacttt ttcggcgcca tgtacttgga aatggctgaa cctgtccaat ctctgatggc
     3241 ggtaaaaagt ctggcaggag cagtggtaga agccagcaaa tctatgaaat accaagggct
     3301 cttcttggaa aaagaaagca gttatttagg cgaattttat aacaaaaaca aggcgaatca
     3361 actgacagat gaggaatttc agctcctact ggactacaat gcctatcttt acaagaaagc
     3421 tgctgagaag attttagcag gccggttcgc catcaatcct tatactgaaa atggcagaag
     3481 cattgcccca tacgtccagc aacatcaggc tattacaggc tttgaagcca attaccatct
     3541 gggccaagcc cgtttcctag aaaaattgga cctagctgat ggcaagcgtc tggtcggaga
     3601 aaaactcaag caagcttggt ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt
     3661 ccctttttaa gtgaggagga gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag
     3721 gaacagaaga aaactgccga acaaatcgaa gctatctaca cttctggtca gaatatcctg
     3781 gtctcagcat cggctggttc tggaaagacc tttgtcatgg tagagcgcat tctggaccaa
     3841 ttggcgcgtg gtgtcgaaat ttctcaactc tttatctcaa cctttaccgt caaggctgca
     3901 actgaactta aagaacgttt agagaaaaaa atcagcaaga aaatccaaga aacagatgat
     3961 gtcgatctca aacaacactt gggtcgccag ttggcagacc tacccaacgc tgccatcgga
     4021 accatggact ctttcacaca aaaattcctt ggcaaacatg gttatctgct tgatattgca
     4081 cctaatttcc gtattttaca aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt
     4141 catgaggtct ttgaagcgca ttaccaaggt aaacagaaag agagctttag tcatttgctg
     4201 aaaaactttg ttgggcgtgg caaggacgaa cggggtctgc ggcagcaagt ctataaaatc
     4261 tatgacttcc tccaatccac cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa
     4321 ggctttgaga aagctgattt taccagtgaa aaagaaaaac tgactgagca aatcaaacaa
     4381 gccctctggg atttggaaag ctttttccgt taccatctgg ataacgatgc caaggagttt
     4441 gcaaaggctg cctatttaga aaatgttcag ttaattctgg atgaaattgg ctccctaaat
     4501 caggagttcg atagtcaggc ttatcaggca gtacttgcgc gtgttgtcgc catctctaag
     4561 gagaaaaacg gtcgtgctct gactaatgcc agccgtaagg ctgatttgaa gcccctggct
     4621 gatgcctaca acgaagagag aaagacccag tttactaaac taggacaatt atcagaccag
     4681 atagcgattc tcgactatca agaacgttat catggagaca cttggaaact agctaaaacc
     4741 ttccaatctt tcatgagcga ttttgtagag gcttatcgtc agagaaaacg acaggaaaat
     4801 gcctttgaat tcgctgatat cagccattac accattgaga ttttagaaaa tttcccacaa
     4861 gttcgtgagt cttatcagga gcgcttccat gaagtcatgg tcgatgagta tcaggatacc
     4921 aaccatattc aagaacggat gctggaattg ttgtctaatg gccacaatcg ctttatggtg
     4981 ggagatatca agcaatccat ctatcgtttc cgtcaggcag atccgcagat tttcaatgag
     5041 aaattccaac gctatgcgca aaatccccaa gaaggcaagc tcattctcct caaggaaaat
     5101 ttccgtagta gttcagaagt gctgtcagca accaatgatg tctttgaacg tctcatggac
     5161 caagaggtcg gcgaaatcaa ctatgataac aagcaccagc ttgtttttgc caataccaaa
     5221 ctgactccca atccagacaa caaggcagaa tttctcctct acgacaagga cgatacaggt
     5281 gaggaagaag agagtcaaac agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag
     5341 gagattctga aacttcatca agaaaaaggt gttgccttta aggaaattgc ccttctgacc
     5401 tccagccgca gtcgtaatga ccagattctc ctcgccctgt ctgagtacgg aattcctgtc
     5461 aaaactgacg gagagcaaaa caattatctc caatccctag aagtgcaagt catgctagac
     5521 actcttcgtg tcattcacaa tcccctgcaa gactacgcct tggttgccct tatgaagtct
     5581 ccaatgtttg gttttgatga ggatgagcta gcacgtttgt cccttcagaa agcagaggat
     5641 aaagtccacg aaaatctcta tgaaaaactg gtcaatgcac aaaaaatggc aagtagtcaa
     5701 aaaggtttga ttcacacagc tctagctgaa aaactaaagc aattcatgga tatcctagct
     5761 tcttggcgct tgtatgccaa aacccactct ctctatgact tgatttggaa gatttacaac
     5821 gaccgttttt attatgacta tgttggggct ttgccgaatg gtcctgctag gcaggccaat
     5881 ctctatgccc tagcactgcg tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg
     5941 cgttttattc gtatgattga ccaagtctta gaagcccagc acgatttggc aagcgtggcc
     6001 gtcgcaccgc caaaagatgc agtagagctc atgaccatcc acaagagtaa agggctggag
     6061 tttccttacg tctttatcct caatatggat caagatttca acaagcagga ctctatgtca
     6121 gaagtcattc tcagtcgtca gaatggtctt ggtgtcaaat atattgccaa gatggagaca
     6181 ggggcagtag aagaccacta tcctaaaacc atcaaactct ccattcctag tctgacctat
     6241 aggcagaacg aagaggaatt acagctagca agctattctg agcagatgcg tttgctgtat
     6301 gttgctatga cgcgggctga gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag
     6361 ctggaagcca agcaataccc agcagccaaa aatgggaaac taaatagcaa tactagactg
     6421 caagcacgga atttccaaga ttggctttgg gctatcagta aagtgtttac taaggacaag
     6481 ctcaacttta gttatcgttt tattggcgaa gatcagttta ccagagaagc tatcggagag
     6541 ttggaaacca agagtcctct ccaagatagc tcccaagcag acaatcgtca gtcagatacc
     6601 atcaaagaag ctctggaaat gctgaaggag gtggaagttt ataatactct tcaccgcgca
     6661 gctattgaac ttcctagtgt tcaaacccca agtcaaatca agaaattcta cgaaccagtt
     6721 atggatatgg aaggtgtcga gattgctggt caaggtcagt cagtaggcaa gaaaatcagc
     6781 ttcgatttgc cagatttttc aaccaaagaa aaggtaactg gagctgagat tggtagtgct
     6841 actcacgaac tcatgcagag aattgacctc agccagcaac taacccttgc tagcctaaca
     6901 gaaacactca aacaagttca aactagccaa gctgtcagag acaagatcaa tcttgataaa
     6961 attcttgctt tctttgacac agtactcggt caggaaattc ttgctaatac cgaccatctc
     7021 tatcgcgagc aacctttctc catgctcaaa cgagaccaaa agagtcagga agactttgtt
     7081 gtccgtggta tccttgatgg ctatctgctt tacgaaaaca aaattgttct gttcgactac
     7141 aagacagacc gctatgatga accaagtcaa ctcgtagacc gctatcgtgg tcagttagct
     7201 ctatacgaag aggctttatc acgagcctat tcgattgaaa atattgaaaa atacttgatt
     7261 ttactcggta aagacgaggt tcaagttgta aaagtataac ttatactctt tgaaaatctc
     7321 ttcaaaccac gtcagcttcg ccttgctgta tatatggtta ctgacttcgt cagttctatc
     7381 cacaacctca aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca
     7441 gtgttttgag ctgattgcgg ctagctttct agtttgctct ttgattttta ttgagtatta
     7501 gaaaggagac ctcatgacac ttccagttag aaaatccctg cacgatgcag ttttacaggc
     7561 ttcgaaagcg gatacttggg aacaagctac caaggaatgg aatgaagttt ccttgatttt
     7621 taacggtatt ggccgtagca attgtgtctg tggaaatgcc atcaaatacg cttatgaact
     7681 ctttaacggt gtcacaggcc aacgcctctt tccaattggt agcgactgtg ttcgtcattt
     7741 tcatcgattg tcccttgacc agcaattgga agaggaagaa aaactgctca gaaaggttga
     7801 aaatctaacc agaaaagctc agaaaaagga aaaaatcaag gtcaataaaa gtgactttga
     7861 cgagcgactt ctaaaatggc tctgggaaaa aggtgttttc aaacccaatc gtggcaatca
     7921 atttgcgcct gagagagact accagctttt cctagaagtc tttcagggag gaagttggac
     7981 caaggcggaa ccaaagaaaa aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc
     8041 ctttttactt gggaaatcgg atgaccaact ctaccttgtc aagctaggca aggagaaaat
     8101 tgactacgaa caagaactcc gtatccaggc agagaaagaa cgtaagaaga gggataaaat
     8161 cgccaagcaa tatgcagaca atctggttct agccatggga cccgcagagc gtgcttatca
     8221 agattacttt ggctttacag agaccctgac tcaagaagaa cgcaagtggg aaaaaattct
     8281 ttttggtaaa aatagagcag aacgggctat caaggccaaa caataccaaa aagagctgga
     8341 aaaagaccaa cgaattgcca gtcaggatcc aattgaaaga aaacagaagc agacttggct
     8401 cctcaattcc tattttcgtg agcttcctga agaaaaagct cgattttcta ggctcttatt
     8461 agaatatcga aaaaatggtg aagtaccctt ttcaactgaa tacctgtcag atcatctcat
     8521 cgactttttc tataaaatga aagcctttga atttgaaatc gcaccagaac aagtccgaga
     8581 ttttctaaaa aaaagcctgc aggaagacca tcgctcatca gcacaaggaa gctggataga
     8641 aggaattctc ctcaactgcc tcaaaccatt tttagaacga ttagtgatat gagtaacacc
     8701 taccgtgtaa atttcatggt aggtgttatt gtacccccaa ataatataaa acaaagtatg
     8761 aaagcgcatc tcaattttaa gacttttttg taaattaaga gtataattaa ggtatactgc
     8821 cttttctata gataatagat aatacactaa tataataaag gtctttgtaa catatataaa
     8881 ttttatttca aggaggaata atggaaaagt attttggtga aaaacaagag cgtttttcat
     8941 ttagaaaatt atcagtagga cttgtatctg caacgatttc aagtttattt tttatgtctg
     9001 tattagctag ttcatctgtg gatgctcaag aaactgcggg agttcactat aaatatgtgg
     9061 cagattcaga gctatcatca gaagaaaaga agcagcttgt ctatgatatt ccgacatacg
     9121 tggagaatga tgatgaaact tattatcttg tttataagtt aaattctcaa aatcaactgg
     9181 cggaattacc aaatactgga agcaagaatg agaggcaagc cctagttgct ggtgctagct
     9241 tagctgctct gggaatttta atttttgctg tttccaagaa aaaggttaag aataaaacgg
     9301 tattacattt agtattggtt gcgggaatag gaaatggtgt cttagtttca gtccatgctt
     9361 tagaaaatca tcttttgcta aattacaata cggactatga attgacctct ggagaaaaat
     9421 tacctcttcc taaagagatt tcaggttaca cttatattgg atatatcaaa gagggaaaaa
     9481 cgacttctga ttttgaagta agtaatcaag aaaaatcagc agccactcct acaaaacaac
     9541 aaaaggtgga ttataatgtt acaccaaatt ttgtagacca tccatcaaca gtacaagcta
     9601 ttcaggaaca aacacctgtt tcttcaacta agccgacaga agttcaagta gttgaaaaac
     9661 ctttctctac tgaattaatc aatccaagaa aagaagagaa acaatcttca gattctcaag
     9721 aacaattagc cgaacataag aatctagaaa cgaagaaaga ggagaagatt tctccaaaag
     9781 aaaagactgg ggtaaataca ttaaatccac aggatgaagt tttatcaggt caattgaaca
     9841 aacctgaact cttatatcgt gaggaaacta tagagacaaa aatacaagaa gaaattcaag
     9901 aaaatcctga tttagctgaa ggaactgtaa gagtaaaaca agaaggtaaa ttaggtaaga
     9961 aagttgaaat cgtcagaata ttctctgtaa acaaggaaga agtttcgcga gaaattgttt
    10021 caacttcaac gactgcgcct agtccaagaa tagtcgaaaa aggtactaaa aaaactcaag
    10081 ttataaagga acaacctgag actggtgtag aacataagga cgtacagtct ggagctattg
    10141 ttgaacccgc aattcagcct gagttgcccg aagcagtagt aagtgataaa ggcgtaccag
    10201 aagttcaacc agcgttatcg gaagcagtcg taaccgacaa aggtgagccc gctgtccaac
    10261 cagagctacc ggaagcagtt gtaagtgata aaggtgaacc agcagtccaa ccagagctac
    10321 cggaagcggt tgtgactgac aaaggtgaga ctgaggttca accagagtcg ccagatactg
    10381 tggtaagtga taaaggtgaa cctaaacagg tagcaccgct tccagaatat acaggaccac
    10441 aagccagcgc tattgttgaa ccagagcagg tagcaccact accagaatac acaggggtac
    10501 aagccggagc tattgttgaa cccgcagtcc agccagcgtt acccgaagca gttgtaagcg
    10561 ataaaggcgt accagaagtt cagcctgagt tgcccgaagc agtagtaagt gataaaggcg
    10621 taccagaagt tcagcctgaa ttacccgaag cagttgtaag tgataaaggt gaaccagagc
    10681 aggtagcacc attgccagaa tataagggga atattgagca agtaaaacct gaaactccgg
    10741 ttgagaaact tcaagaggaa gaaccagaga aaacaataga actaagaagt gtttctgatg
    10801 tggaactcta tagttatgaa gctaatcaat caaaacaaca tacaatctta gataaagtac
    10861 cagataataa atctgcttat ttcatcaaag tgaagtcagc taaatttaaa gatgtactgc
    10921 tcccagtttc aaacattgag gacactacaa aagacggtaa agtagtctat aaaattacag
    10981 ctagattgga acacttaaaa caatctgtag acaatcaata ccaagataat tttacttatt
    11041 atcttccaaa agttgttcaa aacgatggcc ctatctacac atcatttaaa aaattagtaa
    11101 ctgatatgaa tagtagacct gatggaacat ttatacttgg cgcgaccatg aatgctcgtg
    11161 agtttgagtt gggtgaaaat caagaaagtt atgttacaaa gaatttcaca gggacattaa
    11221 ttggtagtca tcaaggtaaa cattatgcta tctataattt gaaaaagcca ttattcaata
    11281 cattaaataa tgctgctatt caggatctca ccttgaaaga tgtcaatatt tctggtaaga
    11341 atcatgtcgc atcagttgct atggaagcca caaattcttc aactttagat aacgttcacg
    11401 caaatggaat cattgctggt gaactaggta tcggaggact tgtccaaaaa gttgataatt
    11461 ctacagtaag aaatagtagc ttcaccggaa gaataacaaa tacttttgtt acaacttctc
    11521 agtatgcaat tggtggtcta gtcggtcaat taaccggtgc taatgctagg gtagctcatt
    11581 ctatttcaac agtagtcatt tcaagtaatg gagaacattc taatcaaaca attggtggta
    11641 ttgttggtat tgtagaaaat aatgcgactg tagaaaatag ttatgctgaa ggtgaaataa
    11701 ataatgctaa accattcgct agagttggtg gagttgcagg ttccctatgg acacaagaag
    11761 gcggaacaga taacaatact ggtcgcttga caaatgttct cagcgatatg aatattacaa
    11821 atgggaatgc gatttctggt taccacttca atggtataaa ggaaacaaat gtctatagta
    11881 ataaagaaaa caaagtagtc aatgttcaag cagttggaga tgaggtaatg tcagttgatt
    11941 ccactgtaga acgtggtact gagattgaaa aaacatcagt tcaagctaag aaagatgaga
    12001 ttactaaaaa acacactatc acagtggaag atttcacaaa ttctgttaaa caggatgtag
    12061 attataccaa ggcagcacat tatc
//
LOCUS       DAVGJJ010000060        11889 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000060 DAVGJJ010000000
VERSION     DAVGJJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.060"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(167..1798)
                     /locus_tag="VVE35_001462"
     CDS             complement(167..1798)
                     /locus_tag="VVE35_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU3061634.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2091..7001
                     /locus_tag="VVE35_001463"
     CDS             2091..7001
                     /locus_tag="VVE35_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU3061635.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEFNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVALSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNKVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQDHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            7091..8287
                     /locus_tag="VVE35_001464"
     CDS             7091..8287
                     /locus_tag="VVE35_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU3061636.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8423..8950
                     /locus_tag="VVE35_001465"
     CDS             8423..8950
                     /locus_tag="VVE35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU3061637.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9027..9383
                     /gene="glnR"
                     /locus_tag="VVE35_001466"
     CDS             9027..9383
                     /gene="glnR"
                     /locus_tag="VVE35_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU3061638.1"
                     /translation="MKEKEFRRNMAVFPISSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9420..10766
                     /gene="glnA"
                     /locus_tag="VVE35_001467"
     CDS             9420..10766
                     /gene="glnA"
                     /locus_tag="VVE35_001467"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU3061639.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10856..10975)
                     /locus_tag="VVE35_001468"
     CDS             complement(10856..10975)
                     /locus_tag="VVE35_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU3061640.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11463..11889
                     /locus_tag="VVE35_001469"
                     /pseudo
     CDS             11463..11889
                     /locus_tag="VVE35_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3810 a         2166 c         2558 g         3355 t
ORIGIN      
        1 tcaggacagt caaatcgatt tctaacaatg ttttagaaat agaggtgtac tattctagtt
       61 tcaatatact atatatctgc agaatactct ttcagtttca ttcagcctag tcttgcttag
      121 gaaaaagtaa gaagccatcc aattatgatg gatagcttct tggttcttag atttgttctg
      181 caaaaacctt ttcagcaagg ttcatagcgt ggtctgatac acgagtgtag tgtgagatga
      241 tgtcgataaa gttgacccca gcttgtgttg aacattcgcc tttgttgagg cgtttgatgt
      301 gggtttttct gagaacacgt tctatcttat tgattgcttc atgacgttct acaagactgc
      361 gtgctttttc aatatcattg ttttccacac tatccagagc atctttgata aagtcactag
      421 tttggcggta aacttcctct aattctttca aggcggcatc agaaaattca acatttttcc
      481 gttgaagata gtcagtcaga ttgagtagag cctccgtgtg gtctccaatc cgttccaaat
      541 cacgggagga atcaaggata ttggtgagca cttcactttc tttttggctg agagcttcgc
      601 ttgaaagggc aatgagataa cgtgttaatt gctcatcgat ggtgttaatt gcttcttcgg
      661 ttttatgccc tttttcagca actttttcat ccaagtcaat gatgtactta taggaaaggt
      721 caaaggcttt agcagcgtag tttcctaagt gcaagagctc tttcttagca tttcctagag
      781 cgatagatgg ggcctgtttg atgaaatgtt catcaagata taagggttcg tattttacaa
      841 cctcgtcctc tccaggaata atcttggtta caaagtaagc cagagctccg ataaatggaa
      901 attggacaat ggtgttggta atattaaagg ttccgtgagc aaaggcgatg gtcatttccg
      961 gtgctagatt tagcgtagct tcaaaccaat gaatcaggac agtaaaagga actagaaaaa
     1021 taacgcagac aactgttccg ataacgttga aggcaacatg agctcctgct acccgtttag
     1081 ctgcaatatt agcccctaaa gaggcaatga tggctgtaat ggttgtcccg atattgtcac
     1141 caaatagaac tggcaaagct ccctgtaggt caattagatt gccggcgtag aggttttgta
     1201 aaatcccaat ggtagccgaa gaagcttgaa ttagcaaggt caagccagta ccgacaaaga
     1261 cacccaaaac aggattttta cttagctcaa tcatatagtc cttaaagacc tgtaaatcct
     1321 tgagtggagc cattgcgccg ctcatgagat tgagggcaaa aaagatacca ccgacaccaa
     1381 agaggatgcg tccgatatta ttgactgtcc gatttttcgt aaaaaagaga cagacggcac
     1441 cgataaagag cataggtagg gcatagttac ctaatttaaa accgatgaga aaggatgtga
     1501 cagttgtccc aatattagca cccatgacaa tcccgatagc ctgacgtaag gttaagagac
     1561 cggcactgac caggccgact gtaataactg ttacaccaga actagactga attagagcag
     1621 tcatcccaat accaaccaga actccaaaga aaggattact agtatatttg tcaatgtaaa
     1681 aacgaaggcg atctccagca gcttgttgca aaccgtctcc catggtcttg atgctatata
     1741 agaatagccc cagaccacct aaaaagtgaa ataaaatttc ctgccaatta atggacattt
     1801 ctttttcctc cgaaaaataa tcgcggaata tctcctattc tattttaaag gataaaagta
     1861 aatctaacaa gtgttaatgt caagtttgta aaagaaaatt ataattgata agtgtattat
     1921 ttttaagagt ttttaacctt attttagatt tgtgcaaaca gttattgttt tttgtctaaa
     1981 aatgttttag gataaaaatg tgattatcat ggttctagag tttgataaaa aactacagaa
     2041 tataaagcgc tttctttaaa acaacaaaat caatctttta ggaggagaaa atgaagaatc
     2101 cattttttga aagacgttgt cgttacagta ttcgtaagtt atcagtagga gcctgctcgc
     2161 tgatgattgg tgctgtttta tttgctggtc cagccttggc tgaagaaact gcagttcctg
     2221 aaaatagcgg agctaataca gagcttgttt caggagagag tgagcattcg accaatgaag
     2281 ctgataagca gaatgaaggg gaacatgcta gagaaaacaa gctagaaaag gcagaagcag
     2341 ctagcgcagc taaaccagag gaaaaagcag gtgaggtggt tgcagaaaca ccatctgcag
     2401 aagcaaaacc taagtctgac aaggaaacag aagcaaagcc cgaagcaact aaccaagggg
     2461 atgagtctaa gccagcagca gaagctaata agactgaaaa agaagtccag ccagatgtcc
     2521 ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa atttaattct tgggaagaat
     2581 tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat taaccgcgga tctgttgtcc
     2641 tcgcttcacg tcggacaggt catttagtca atgaaaaagc tagcaaggaa gcaaaagttc
     2701 aagccttatc aaacaccaat tctaaagcaa aagaccatgc ttctgttggt ggagaagagt
     2761 tcaaggccta tgcttttgac tattggcaat atctagattc aatggtcttc tgggaaggtc
     2821 tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa cggggttcct gtatacggta
     2881 cactcttctt caactggtct aatagtattg cagatcaaga aagatttgct gaagctttga
     2941 agcaagacgc agatggtagc ttcccaattg cccgtaaatt ggtagacatg gccaagtatt
     3001 atggctatga tggctatttc atcaaccaag aaacaactgg agatttggtt aaacctcttg
     3061 gagaaaagat gcgccagttt atgctctata gcaaggaata tgctgctaag gtaaaccatc
     3121 caatcaagta ttcttggtat gatgccatga cctataacta tggacgttac catcaagatg
     3181 gtttgggaga atacaactac caattcatgc aaccagaagg agataaggtt ccggcagata
     3241 acttctttgc taactttaac tgggataagg ctaaaaatga ttacactatt gcaactgcca
     3301 actggattgg tcgtaatcct tatgatgtat ttgcaggttt ggaattgcaa cagggtggtt
     3361 cctacaagac aaaggttaag tggaatgaca ttttagacga aaatgggaaa ttgcgccttt
     3421 ctcttggttt atttgcccca gataccatta caagtttagg aaaaactggt gaagattatc
     3481 ataaaaatga agatatcttc tttacaggtt atcaaggaga ccctactggt caaaaaccag
     3541 gtgacaaaga ttggtatggt attgctaacc tagttgcgga ccgtacgcca gcggtaggta
     3601 atacttttac tacttctttt aatacaggtc atggtaaaaa atggttcgta gatggtaagg
     3661 tttctaagga ttctgagtgg aattatcgtt cagtatcagg tgttcttcca acatggcgct
     3721 ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata tgattttaca gatgcctata
     3781 atggcggaaa ttcccttaaa ttctctggtg atgtagccgg taagacagat caggatgtga
     3841 gactttattc tactaagtta gaagtaactg agaagaccaa acttcgtgtt gcccacaagg
     3901 gaggaaaagg ttctaaagtt tatatggcat tctctacaac tccagactac aaattcgatg
     3961 atgcagatgc atggaaagag ctaacccttt ctgacaactg gacaaatgaa gaatttgatc
     4021 ttagctcact agcgggtaaa accatctatg cagtcaaact atttttcgag catgaaggtg
     4081 ctgtaaaaga ttatcagttt aacctaggac aattaactat ctcggacaat caccaagaac
     4141 cacaatcgcc gacaagcttt tctgtagtga aacaatctct taaaaatgcc caagaagcgg
     4201 aagcagttgt gcaatttaaa ggcaacaagg atgcagattt ctatgaagtt tatgaaaaag
     4261 atggagacag ctggaaatta ctaactggct catcttctac aactatttat ctaccaaaag
     4321 ttagccgctc agcaagtgct cagggtacaa ctcaagaact gaaggttgta gcagtcggta
     4381 aaaatggagt tcgttcagaa gctgcaacca caacctttga gtgggggatg actgtccaag
     4441 acacaagtct tccaagacct ctagcagaaa acatcgttcc aggggctact gttatcggaa
     4501 gtaccttccc agatacagaa ggtggagaag gtattgaagg tatgttgaac ggtaccatta
     4561 ctagcttgtc agataaatgg tcttcagctc agttgagtgg tagtgtggat attcgtttga
     4621 ccaagccacg taccgttgtt agatgggtca tggatcatgc aggggctggt ggtgagtctg
     4681 ttaacgatgg cttgatgaac accaaagact ttgaccttta ttataaagat gcagatggtg
     4741 agtggaagct agctaaggaa gtccgtggca acaaagcaca cgtgacagat atcactcttg
     4801 ataaaccaat cactgctcaa gactggcgct tgaatgttgt cacttctgac aatggaactc
     4861 catggaaggc tattcgtatc tataactgga aaatgtatga aaagcttgat actgagagtg
     4921 tcaatattcc gatggccaag gctgcagccc gttctctagg caataacaag gtacaagttg
     4981 gctttgcaga tgtaccggct ggagcaacta ttaccgttta tgataatcca aattctcaaa
     5041 ctccactcgc aaccttgaag agcgaagttg gaggagacct agcaagtgca ccattggatt
     5101 tgacaaatca atctggtctt ctttattatc gtacccagtt gccaggcaag gaaattagta
     5161 atgtcctagc agtttccgtt ccaaaagatg acagaagaat caagtcagtc agcctagaaa
     5221 caggacctaa gaaaacaagc tacgccgaag gggaggattt ggaccttaga ggtggtgttc
     5281 ttcgagttca gtatgaagga ggaactgagg acgaactcat tcgcctaact cacgcaggtg
     5341 tatcagtatc aggttttgat acgcatcata agggagaaca gaatcttact ctccaatatt
     5401 tgggacaacc agtaaatgct aatttgtcag tgactgtcac tggccaagac gaagcaagtc
     5461 cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa aaaagattac ctagttggtg
     5521 atagcttaga cttgtctgaa ggacgctttg cagtggctta tagcaatgac accatggaag
     5581 aacattcctt tactgatgag ggagttgaaa tttctggtta cgatgctcaa aagactggtc
     5641 gtcaaacctt gacgcttcgt taccaaggtc atgaattcaa ttttgatgtt ttggtatctc
     5701 caaaagcagc attgaacgat gagtacctca aacaaaaatt agcagaagtt gaagctgcta
     5761 agaacaaggt ggtctataac tttgcttcac cagaagtaaa agaagctttc ttgaaagcaa
     5821 ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag cacccaagat caagtcaatg
     5881 accgacttaa taaattgaca gaagctcata aagctctgaa tggtcaagag aaatttaagg
     5941 aagaaaagac agagcttgat cgcttaacag gtgaggttca agaactcttg gctgccaaac
     6001 caaaccatcc ttcaggttct gccctagctc cgcttcttga gaaaaacaag gccttggttg
     6061 aaaaagtaga tttgagtcca gaagagcttg caacagcgaa acagagtcta aaagatctgg
     6121 ttgctttatt gaaagaagac aagccagcag tcttttctga tagtaaaaca ggtgttgaag
     6181 tacacttctc aaataaagag aagactgtca tcaagggttt gaaagtagag cgtgttcaag
     6241 caagtgctga agagaagaaa tactttgctg gagaagatgc tcatgtcttt gaaatagaag
     6301 gtttggatga aaaaggtcaa gatgttgctc tctcttacgc ttctattgtg aaaatcccaa
     6361 ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc tgaaggcaaa gaggcagtag
     6421 aattggcttt tgaacaaacg gatagtcatg ttatctttac agcaccacac tttactcatt
     6481 atgcctttgt ttatgaatct gctgaaaaac cacaacctgc taaaccagca ccacaaaaca
     6541 aagtccttcc aaaacctact tatcaaccgg cttctgatca acaaaaggct cctaaattgg
     6601 aagttcaaga ggaaaaggtt gcctttcatc gtcaagatca tgaaaatgct gagatgctag
     6661 ttggggaaca acgagtcatc atacagggac gagatggact gttaagacat gtctttgaag
     6721 ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt catccaagaa gcgattccag
     6781 aaattgttga aattggaaca aaagtaaaaa cagtaccagc agtagtagct acacaggaaa
     6841 aaccagctca aaatacagca gttaaatcag aagaagcaag caaacaattg ccaaatacag
     6901 gaacagctga tgctaatgaa gccctaatag caggcttagc cagccttggt cttgctagtt
     6961 tagccttgac cttgagacgg aaaagagaag ataaagatta aatatcgaaa aatcttgtga
     7021 aatctttcct tatatttcca aagtgtgata taatagtttc gaataaaata aataaaggag
     7081 tttttgtaac atggcaaaac ttactgttaa agacgttgac ttgaaaggta aaaaagtcct
     7141 cgttcgtgtt gacttcaacg taccattgaa agatggcgta atcactaacg ataaccgtat
     7201 cacagcagct cttccaacta ttaagtacat catcgaacaa ggtggacgtg caattctttt
     7261 ctctcacctt ggacgtgtga aagaagaagc tgataaagct ggtaaatcac ttgctcctgt
     7321 agcagctgac ttggcagcaa aacttggtca agatgttgtt ttcccaggtg tcactcgtgg
     7381 tgctgaattg gaagcggcaa tcaacgctct tgaagatgga caagttctct tggttgaaaa
     7441 cactcgttac gaagatgttg acggcaagaa agaatctaaa aacgatcctg aacttggtaa
     7501 atactgggca tcacttggag atggtatctt cgtaaacgat gcattcggta cagctcaccg
     7561 tgcacacgca tctaacgttg gtatctcagc aaacgttgaa aaagcagttg ctggtttcct
     7621 tcttgaaaac gaaattgcct acatccaaga agcagttgaa actccagaac gtccatttgt
     7681 ggctatcctt ggtggttcaa aagtttcaga caagatcggt gttatcgaaa acttgcttga
     7741 aaaagctgat aaagtcctta tcggtggtgg gatgacttac acattctaca aagcacaagg
     7801 tatcgaaatc ggtaactcac ttgtagaaga agacaaactg gatgttgcga aagctcttct
     7861 tgaaaaagca aatggtaaat tgatcttgcc agttgactca aaagaagcta acgcatttgc
     7921 tggttacact gaagtgcgtg acactgaagg tgaagcagtt tctgaaggct tccttggtct
     7981 tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct ttgactggtg ctaaaacagt
     8041 tgtatggaac ggacctatgg gtgtatttga aaacccagat ttccaagctg gtacaatcgg
     8101 tgtgatggac gctatcgtga aacaaccagg agttaaatca atcatcggtg gtggtgactc
     8161 agctgccgca gcgattaacc ttggccgtgc agacaagttc tcatggatta gtacgggtgg
     8221 tggagcatca atggaacttc ttgaaggtaa ggttcttcca ggacttgcag ccttgacaga
     8281 aaaataagat tttataaata aatcaaagaa gagagggatg aaagttcctc ttttcttttg
     8341 cttaaaataa aaacgcttcc tctcaactat tactcataaa aatcaccgat ttatgataaa
     8401 atggaaatag aaagttgaga ttatgagtta ttttaaaaaa tataaattcg ataaatccca
     8461 gttcaaactt ggtatgcgaa cctttaaaac aggtattgct gtttttctag ttctcttgat
     8521 ttttggcttt tttggctgga aaggtcttca aattggtgct ttgacagccg tttttagcct
     8581 gagggagagt tttgatgaga gtgttcattt tgggacttcg cgtattctag gaaatagtat
     8641 cggtggactc tatgccttgg tcttcttctt attaaatacc tttttccacg aagccttttg
     8701 ggtgaccttg gtagttgttc caatctgcac catgttaacc attatgacaa atgtagccat
     8761 gaataacaaa gcaggggtta ttggtggtgt agcagctatg ttaatcatta ccctatcaat
     8821 tccaagtggt gagacaattt tgtacgtgtt tgtgcgtgta ttagaaacgt ttatgggagt
     8881 ttttgtcgca attatcgtaa attacgatat tgatcgtatt cgtctctttt tagagaaaaa
     8941 agaaaaataa tgttacattt tataacatta tcaattgacg tttgtctttt tttagactat
     9001 aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga atttcgccga aatatggctg
     9061 tttttcctat cagcagtgtt atgaagttga ccgatctatc ggcgcgtcag attcgttatt
     9121 atgaagatca agagttgatc aagcccgatc gaaacgaagg aaatcgtcgc atgtattcct
     9181 tgaatgacat ggatcgtctg cttgaaatca aagattatat ctctgaaggt tataatatcg
     9241 ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc caagaaagcg gttagtcaga
     9301 ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca ggggcgtttt gcttcagtac
     9361 agtcaccttt tggtcgcggt taggcaaccg caagtagtca tagattaagg agaaaactta
     9421 tgccaatcac agctgcagat attcgtcgtg aagtcaagga aaaaaatgtt acctttattc
     9481 gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt cgaaattcct gctacagatg
     9541 aacagttaga taaggtcttg tcgaacaagg ttatgtttga tggatcttct attgaaggtt
     9601 ttgtacgtat caatgagtcg gatatgtact tgtacccgga cttggataca tggacagtct
     9661 tcccttgggg agatgaaaat ggaagtgttg caggtctgat ctgtgatgtc tatacaacag
     9721 aaggtgaacc atttgcgggt gaccctcgtg gtaatttgaa acgagctctt cgtcacatgg
     9781 aagaagttgg attcaaatcc ttcaatcttg gtccagagcc agaattcttc ctatttaagt
     9841 tggatgaaaa tggggaccca acacttgaag tgaatgacaa gggtggctac tttgatttgg
     9901 cacctactga ccttgcggac aacacacgtc gtgagattgt gaatgtcttg accaaaatgg
     9961 gatttgaagt agaagcgagt caccacgagg ttgcggttgg acagcatgag attgacttta
    10021 agtacgatga agttctccgt gcttgtgata agattcaaat ctttaaactt gttgttaaaa
    10081 ccattgctcg caaacacgga ctttacgcaa catttatggc gaagccaaaa tttggtattg
    10141 ctggatcagg tatgcactgt aatatgtcct tgtttgatgc agaaggaaat aacgccttct
    10201 ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac agcttaccat ttcctaggcg
    10261 gtttgatcaa gcatgcttac aactatactg ccatcatgaa cccaacagtt aactcataca
    10321 aacgtttggt tccaggttat gaagcgcctg tttacattgc ttgggctggt cgtaaccgtt
    10381 cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac tcgtcttgag ttgcgttcag
    10441 tggatccaat ggcgaaccct tacgttgcta tggctgttct tttggaagtt ggtttgtatg
    10501 gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga aaatatctac atcatgacag
    10561 cagaagagcg caaggaagct ggtattacag accttccatc aactcttcac aacgctttga
    10621 aagctttgac agaagatgaa gtggttaaag ctgctctcgg agatcacatc tacactagct
    10681 tccttgaagc caaacgaatc gaatgggcaa gttatgcaac cttcgtttca caatgggaaa
    10741 ttgataatta tttagacctt tactaatata gacgaaagat tgcctgtggg taaacagtca
    10801 cacagcagtc taagccatca aatacctata acccttaaaa tagcgatttc ggtcgctatt
    10861 tatttttcga aaaatcctct tgactctttt gcgaattaga tttatcatca gacgtgtaga
    10921 agaaaatcaa ggagtcacta tggaaaaatt caatttcaaa aacaatatcg ggcaagaaaa
    10981 caagttactt caaatcgaaa tctacaagtt tactaacttt tgcaagcttc aaaattacac
    11041 atctgtaaat attttttcga aagatatatt tgaagctata gtaaattgag actagaatag
    11101 tacactacat ctgctaaaaa tttctagaaa ctaatttaac tttcctaatc gatttgttta
    11161 catcttattt caatctacta tattatctca ataaatgatg atggcgactg ttttgtttgt
    11221 gcttgtcgat ttttgaatcc tgatcgagcg gaagaatatt tctgccgagg ttgccaaggg
    11281 gaaaatcctg aagatattga attttatgat gaacaattac aggcagaaaa ggtggaggtt
    11341 ttgaatattc tattggaagt aaaaaattga attatctaca atctcatcaa caataaacct
    11401 atttcaaccc cattatctat aggcgtattt ttacgccttt ttatatactt gaagcacata
    11461 agaaacatta tgggaagttt gctgatggaa gtactcaaga aattgatgtt ccttatgata
    11521 ttcctgatac ttgggagtgg gtgaggataa aatcaattta ttggaatttt gggcaaaata
    11581 agccagagaa atcctttagg tatatagata cgtctagtat tgatagaaaa aagaacataa
    11641 tcaactacaa aaatctacaa tatctttcac ctgaacaagc gccttcccgt gctagaaaat
    11701 tagtttcgca gaatagtgtc ttattttcaa cagttagacc atatctaaaa aatattgctg
    11761 tagttagaga acttaaagag tatttgatag ctagtacagc atttattgtt ttggatactt
    11821 tacttaacga aacatatttg aaatattatt tattatcaga taattttatc aatcgtgtta
    11881 ataataaat
//
LOCUS       DAVGJJ010000061        11552 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000061 DAVGJJ010000000
VERSION     DAVGJJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.061"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..223
                     /locus_tag="VVE35_001470"
     CDS             <1..223
                     /locus_tag="VVE35_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061641.1"
                     /translation="FKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMVNSN
                     PYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            233..931
                     /locus_tag="VVE35_001471"
     CDS             233..931
                     /locus_tag="VVE35_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061642.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAIVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            928..1575
                     /locus_tag="VVE35_001472"
     CDS             928..1575
                     /locus_tag="VVE35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061643.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1663..2529)
                     /locus_tag="VVE35_001473"
     CDS             complement(1663..2529)
                     /locus_tag="VVE35_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3061644.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAKDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(2541..3299)
                     /locus_tag="VVE35_001474"
                     /pseudo
     CDS             complement(2541..3299)
                     /locus_tag="VVE35_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3300..3977)
                     /locus_tag="VVE35_001475"
     CDS             complement(3300..3977)
                     /locus_tag="VVE35_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3061645.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     TKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(3937..4617)
                     /locus_tag="VVE35_001476"
     CDS             complement(3937..4617)
                     /locus_tag="VVE35_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU3061646.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKKTSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSGK"
     gene            4833..4979
                     /locus_tag="VVE35_001477"
     CDS             4833..4979
                     /locus_tag="VVE35_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU3061647.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            5107..6597
                     /gene="lysS"
                     /locus_tag="VVE35_001478"
     CDS             5107..6597
                     /gene="lysS"
                     /locus_tag="VVE35_001478"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU3061648.1"
                     /translation="MSIEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREIAGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            7221..8357
                     /gene="lctO"
                     /locus_tag="VVE35_001479"
     CDS             7221..8357
                     /gene="lctO"
                     /locus_tag="VVE35_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU3061649.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      8611..8699
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8914..9582
                     /locus_tag="VVE35_001480"
     CDS             8914..9582
                     /locus_tag="VVE35_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU3061650.1"
                     /translation="METQDYAFQPGLPVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     gene            complement(9732..9818)
                     /locus_tag="VVE35_001481"
                     /pseudo
     CDS             complement(9732..9818)
                     /locus_tag="VVE35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9909..10691
                     /gene="thiM"
                     /locus_tag="VVE35_001482"
     CDS             9909..10691
                     /gene="thiM"
                     /locus_tag="VVE35_001482"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU3061651.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGAFKVEFINALYEITDEDVKEFKRVK"
     gene            10693..11322
                     /locus_tag="VVE35_001483"
     CDS             10693..11322
                     /locus_tag="VVE35_001483"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU3061652.1"
                     /translation="MFHKELLRLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNDEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
BASE COUNT         3595 a         1966 c         2454 g         3537 t
ORIGIN      
        1 gtttaaaaaa tcaaacatga gcttagtatt tctgtttact tttttatttg tagaatcctt
       61 attctggatt tatcagttgg acaatgggat aattggatta ttgccaattt ttcagtatat
      121 ggtaaattcc aatccgtatg cattgattta ttggcttaca ttactatcta tcataattcc
      181 attgactgta ttttctgttc atagaaactg gaggagagtg taaaagttgg aaatgggaaa
      241 gttaagtagt cacatgtgga ggttgaatca gataatctat accaagtact tttggggtta
      301 tgttcttttt tggatattga tttgtttagg attatggtat tggttagaag gaaatgatag
      361 acttgttata gaaattttaa aagggcctaa tctgagtcaa aactcttttt tagtcttatc
      421 tatatggttg cttcattggt ttattattca tacatttttt ctagcaattg tatatcgtag
      481 aagagcatcc gatttcttta tggaagtgat tcgattttct tctattaagc tctggattag
      541 gtatcagatt tggacctgtt ttctttatgg acttatttta atcatggtaa aagttctagt
      601 gattcaattt atgttacagt taccaaactg ggatatagga gttttgttta tagttgattc
      661 tttgaatgct tgtgtgttag tcttgttttg ctttatgtta tacgcactag gagcgaatgt
      721 acaaatgaac tttgcttgcg ttagtttctt tttactcatg attgtgtttg gtggtctatt
      781 tgtaggaaat cgaactaatt atctatttta tattttgaat cgagggaatg gagatatagg
      841 acgagattta tttcttcagt tgctattttt agtctttctt tttcaaagca ttttctattt
      901 cactcgtcaa aagaggaggt ttatagaatg aatgaaataa ttacattaaa aaatatccag
      961 ttggaattaa aaaaaacatg tgtttttcaa aaccttaatt ttagttgtaa acagggggaa
     1021 attataggaa ttactggtgc gaatggctca gggaaaagtg tattgtttaa attaatagct
     1081 ggtttatata gtccgtctta tggagaagtg ttaatcaatg gggaaaatat tgttcctgag
     1141 agaaaaattc cagctaattt gggagctttg attgaagaac ctggttttat aaattattat
     1201 agtggcttta agaatttaca atatttggca agcatacgag gagtagttgg taatcaggaa
     1261 atcaatgata cactgaaaat agttggtcta tatgagcaaa aagaccagaa agttaaaact
     1321 tattcgctag gtatgaggaa aaagctaggg attgctcaag caattatgga gaatccctct
     1381 attcttttac tagatgaacc tatgaatgcc ttggataaat caagtgtaga aaatatgaga
     1441 acattgttta gaaagctctc tagtgaaaaa gggacaacaa ttttgattgc tagtcatagt
     1501 gaagaggata ttcgtatctt atgtgataaa gtatatgcaa tagaagataa agtatgtaca
     1561 ctgtgttcag attgaacatc ttagatagac tcaggatgtt ataggtaaaa tcttaaaaag
     1621 ggattggatc ttaaaatcca gttccttttt aagagttaat gactaatcaa cttttccacc
     1681 ttcaacaacc aggtcatctg ctttagcagc gtcaccgtag gttgggtgaa gtgttttttc
     1741 ataggccttg tggaagaagt tttccttgcc taatttttca atatctttat tgatgaagtc
     1801 tagcaattct tggttgcctt tttgaactgc tgccgcaatg gtatcgggat caccgaggga
     1861 agtaattcct acttcaaatc ctttattttc aagcgcccaa gctagaactt ccgtattgtc
     1921 agttgaaaag gcatctccac gtccgtcaag aagagcttgg taagagtcac tgtattggtc
     1981 gtatttttgg agtttgattt ctggatgatt cttttcaaaa taagtctcag cagtcgttcc
     2041 ttttgtgaca attaaggttt taccttcaag ttgtttgacg tctgtaatga gaccagtctt
     2101 aggtgatacg acacccagag aaactttcat atatggaagg gcaaaatcaa cttgtttctt
     2161 acgttcgtca gttactgtaa agttagcaag agtaatatct accttgtttg aaatcaagta
     2221 ttccgcacgg ttggcagcat cgactgaaat gtatttaacc ttgacaccaa ggtctttagc
     2281 tagttggttc cctagttcaa tatcgtagcc ttggtaagaa ccatcattgt caacgtagcc
     2341 aaacggtttt ttatctccaa acacggcgat tcgcagttca ccgctttttt tgatttcatc
     2401 gatagtgcga gctttggcag ttgtttttcc agaggatgaa ccagcgtttc cacctgagct
     2461 acaagcagtg ataaagataa gggcaaaggc aagtgctaaa acagttaaga gtggtttgaa
     2521 tagtttcata agaatctcct ttatagatat gagccaaatt ggctaaagtc aaagacgttt
     2581 aaaaattcct gggctcgttt ggtttgcgga ttggtaaaga aggcttgagc tgttccttct
     2641 tcagcgattt tcccttggtc gaggaagata atccggtcag caatggcttg ggcaaactgc
     2701 atttcgtggg ttactaaaat catggtacgg ccttcttggg ccaaatcatt gataagttcc
     2761 agcacctcac gcaccatttc tggatccagc gaagcagtca cctcgtcaaa aaggatgatt
     2821 tctggatgca ttaggagggc acggacaatt gcaacacgtt gcttctgtcc accagataat
     2881 tgacgggcaa agctatgttg tttatccagc aaaccgacac gatctagtaa ttgcaaagct
     2941 tcttccgtta cttctttctt gtcccatcct tgagttttga tagggcctag gatgaggttt
     3001 tgtaagacat ccagatgggg aaagagttca taactttaaa agaccatgcc aatcttttgg
     3061 cgaactagct gaaaatcttt tttattttca acgatagact gaccatccag aagaatatct
     3121 ccaccttgaa tactttctaa gccgttgagg caacgaagga gggtactttt cccacaacca
     3181 gatggcccta ggatgacaac aacttcccct tttttgattt ctagagaaag tccttggagg
     3241 atgggattgt ctccgaagga tttttttagt tccttgattt ctaagatagt ttcagacatt
     3301 tagtttctcc aatgtttttc taagtgagtg gatagtttgg aaatagggta gcaaactgcg
     3361 aaatataaga ctagaatggt tccataaatc caaaatgaag cagttgggat ggtcaggcga
     3421 ttgctatcga tgatttgttg tccaactttg gtcacttcca caaccccaat caaaacaact
     3481 aatgaagtgg ttttagtcat ccgagtgaca agattgatag cctgcggtag cagtcttctt
     3541 aagacttgtg ggatgatgat gtggtagtaa agttgaacat tagtcaagcc gagtgcctgt
     3601 ccactttcaa actgatgttt agggagagaa gtgatagctc cacgtaccaa gtctcccatt
     3661 tcagctgctc cccagagggt aaaaacgata atagctgaag tctcacctga gatattgata
     3721 ttaaagtttc gagctaagcc aaagtaaacg atgaagagta gcaccagctg gggcatgata
     3781 cgggtaaagt ccagatacaa tcgtgttaaa aatcgtatga ttctagaatg ggaggtcatg
     3841 atgattccca tgactgttcc gaacatcatg gataagagga cagacaggat ggatatctca
     3901 atcgtaacgc ccaatccctg taagattctc aggagattat ttccctgaaa gagtacttgg
     3961 attcccgaat cctgcatggc ggagcctcct ttctatccag ctaaagacca gtgagatggg
     4021 tagcagcatg atcagataag caactaccaa catagctagc gcaatgtctg tctcatagta
     4081 gagaccaatc aaatccttgg cgacatacat gaggtcggcc aaagccactg ctgagaaaac
     4141 agaggttttc ttgataagga aaatgacatt ggcactaaag gaggggagtg ccaccgctgt
     4201 tgcttgcgga agaaccacat agcgaaagac ctgtagaggt gtcagaccaa tagcgaggcc
     4261 aatctcctgc tgggtttgac tgatggcttc cagcccactt cggaaagatt ctgccatata
     4321 ggaacctcct aaaaagacaa gcccaagcgt tgcacagact tctgaagata ggacaatccc
     4381 gattcgggga agaccgaagt agagaaagaa gagttgaatc aaaaggggcg tattacgtga
     4441 caattcaatg taggctgtcg ctacttgcgc caaaacaggg attcgataat gtcggatgat
     4501 actaacgatt aaaccgagca gaaaggatcc caaaattccc caaactgcaa tatgcaaggt
     4561 cagaaagaat gccttttgat atagtggtag atattgttca acaatggacc aatccaaaaa
     4621 tagaacctcc catctagaaa taatacagtt attgtagcac ttaaaatctt ctttggataa
     4681 tatctatttt ttattgccgt tataaggatt ttttaccata gacataaaat ttctgaaatt
     4741 tccaaacaaa atatttgaaa agttttgaaa aaagagttaa gatattttta taatatacaa
     4801 agtaaacgct tacttattaa ggaggacatt ttatgtcata caaaacaagc aatgcagaag
     4861 gtcatgtaga tttcatcaat acctatgatt tggagccaat ggcgcaacaa gttattccta
     4921 aagcagcatt tggctatatc gctagtgggg cggaagatac tttcacttct ttccagtgat
     4981 tttagcgtca ggttcttttt agtttttaaa gattatccgt gaatttcttg cttatttatg
     5041 ataaaatggg agtatcgcaa aaaatgactc atcgtattca attttgagta aaactaggag
     5101 gatcccatgt ctatagaaca tatggaagaa ctaaatgacc agcagatcgt tcgccgtgaa
     5161 aaaatggctg cgctccgcga acaaggaatc gatcctttcg gaaaacgttt tgaacgtact
     5221 gcaaattcac aagaattaaa agataaatat gccaacctcg ataaagaaca attacacgat
     5281 aaaaacgaaa cagctactat cgcaggacgc ttggtaacca aacgtggtaa aggaaaagtt
     5341 ggttttgccc accttcaaga ccgcgaaggc cagattcaga tctacgttcg taaggatgct
     5401 gtcggtgaag aaaactacga aatcttcaaa aaagcagacc ttggtgactt ccttggtgtc
     5461 gaaggtgaag tgatgcgtac ggatatggga gaactctcta tcaaggcaac ccacatcaca
     5521 cacttgtcta aggctcttcg tcctcttcct gagaaattcc atggtttgac agacgttgaa
     5581 acaatttacc gtaaacgtta ccttgacttg atttctaatc gtgaaagctt tgaacgcttt
     5641 gtcactcgtt caaaaatcat ctctgaaatc cgtcgttacc ttgaccaaaa aggattcctt
     5701 gaagtggaaa cacctgttct tcataatgaa gccggtggtg ctgctgcccg tccatttatc
     5761 acccaccaca atgcccaaaa cattgacatg gtgcttcgta tcgcgactga gcttcactta
     5821 aaacgcctta tcgtgggtgg tatggaacgt gtctatgaaa ttggccgtat cttccgtaac
     5881 gaaggaatgg atgctactca taaccctgag ttcacttcta tcgaagttta ccaagcttat
     5941 gcagacttcc aagacatcat ggacttgact gaaggtatta tccaacacgc tgctaaatca
     6001 gtcaaaggtg atggcccagt caactaccaa ggtactgaaa tcaagattaa cgaaccattt
     6061 aagcgtgttc atatggtgga tgctatcaga gaaattgctg gtgtcgattt ctggcaagac
     6121 atgactttgg aagaagctaa agctatcgct gctgagaaga aagttccagt tgagaaacac
     6181 tacactgagg ttggtcacat catcaatgcc ttctttgaag agtttgttga agaaacttta
     6241 atccaaccaa cctttgtcta tggacatcca gtagctgtat ctccactcgc taagaaaaat
     6301 cctgaagacc aacgctttac tgaccgtttc gagctcttta tcatgactaa ggagtacggt
     6361 aatgccttta ctgagttgaa cgacccaatc gaccaactta gccgttttga agcccaagct
     6421 aaagctaaag aacttggtga tgatgaagcg acaggaatcg actatgacta catcgaggct
     6481 cttgaatacg gtatgccacc aacaggtggt ttgggaatcg gtatcgaccg tctctgcatg
     6541 ctcctcactg atacaacaac tatccgtgat gtattactct tcccaacaat gaaataaatt
     6601 cttatcctct ggctcttacc agaggatttt ttgattcaaa aagagactga atttaaggag
     6661 aaaatgaagt gtagtatatt gaagttgaaa tagtacactt tgatttctaa gacattgtta
     6721 gaaattggtt taaattccct aatcaatttg tgcatgtttt atttcatttt acgatagtac
     6781 gctgaaactg ttcaaaaagt actagaaatt gacttggatt ccccaattga tttgttcaga
     6841 ttcactataa ataaaaaatt aataagtggg ataggaagtt agcgtcaact aggatagtat
     6901 cttgcttaaa cagtatatat gggactgata taagtccata ggtcctatta gaggatgttc
     6961 tggtgtctta ttcacttgtt tttttatagt attagtagat agaatccgca aataaaaaag
     7021 tagttcaaat gcctggcagc aaaaaatctc aacgattcac ttaatataga ctaaggttta
     7081 gagtttaagg aaagtagggc tagtatattg tatgaaaaaa taagatggtt tttaagcaaa
     7141 actcttgaaa atgattgaaa aaagagttat tatagttgta tagtaaaaag caaacgctta
     7201 cttattaagg aggacatttt atgtcataca aaacaagcaa tgcagaaggt catgtagact
     7261 tcatcaatac ctatgatttg gagccaatgg cgcaacaagt tattcctaaa gcagcatttg
     7321 gctatatcgc tagtggggcg gaagatactt tcactttaag agagaatatc cgcgccttta
     7381 accacaaact catcgttcct catacacttt gcgatgttga aaatccaagt acagagattg
     7441 aatttgcagg tgaaaaacta tcttcgccaa tcattatggc acctgttgcg gctcataaat
     7501 tggcaaatga acagggggaa gtggcgactg cgcgtggtgt gcatgagttt ggttctcttt
     7561 atacaaccag ttcttactct actgttgacc ttccagagat ttctgaagcc cttcaaggga
     7621 cacctcattg gtttcaattt tactttagta aggatgacgg tatcaaccgc cacatcatgg
     7681 accgtgtgaa ggctgaaggt tataaagcga ttgtcttgac ggcagatgct actgtagggg
     7741 ggaatcgtga agtggataag cgtaatggtt ttgtcttccc agttggcatg ccgattgttg
     7801 aagaatacct gccagaaggt gctggtaaat caatggactt tgtttacaaa tcagctaaac
     7861 aacgcttgtc tccacgtgat gtagaattta tcgctgaata ctcaggactt cctgtgtatg
     7921 tcaagggacc acaatgccgt gaggacgttg aacgttcgct tgctgcagga gcttctggta
     7981 tctgggtaac caaccacggt ggtcgtcaaa tcgacggtgg accagctgcc tttgactcgc
     8041 ttcaagaagt ggcagaagca gttgataaac gtgtgccaat tgtctttgac tctggtgttc
     8101 gtcgtggtca acatgtcttt aaagccttgg cttcaggagc agacttggta gctattggtc
     8161 gcccggttat ctatggcttg gctctcggcg gtagtgttgg tgtgcgtcaa gtctttgaac
     8221 acttgaatgc ggaattgaag acagttatgc agttgtctgg aactcagact attgaagatg
     8281 ttaaacactt caaactccgt cacaacccat acaacccaac tttcccagtt gaccctcgtg
     8341 acttaaaatt gtattgataa aagagattgc ctccactgaa tgtggaggtt tttcttcgtg
     8401 tattggaaga ataaatcgct atcgaaaacc atagtaaaat ggaataaaaa caggacaaat
     8461 caatcaggac agttaaatcg atttttaaca atgttttaga agtagatgtg tactattcta
     8521 gtttcaatct actatatata aagatatttt tcggtcaaat atcttgctag ggctttcatt
     8581 ttttgctata ctggttcagt aattaaataa tagagacatt tggggtgctt taagctgaga
     8641 tgatacccat tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa acgttttttc
     8701 gtatggcgaa atgaacgatc taatcttgta agtcaactct aattcttgat tgtaattgga
     8761 gttttttgtt tatataaact ggataggctt gtttagttag ctctctgcaa atgctctcga
     8821 tgtgctttaa aaggatcaag tggaattacg attttgcttg atatttcttg ctgttttatt
     8881 aattactaac aagtaaagag aggagaagta aatatggaaa cacaagacta tgcatttcag
     8941 ccaggtttgc ctgttggaga attattaaaa agcagtcaga aggattggca ggctgcaatc
     9001 aatcatcgtt ttgttaagga actttttgcg gggacaattg agaataaggt cttaaaggac
     9061 tatctgattc aagattatca cttctttgat gccttcttat ccatgctggg tgcctgcgta
     9121 gctcacgcag ataaacttga atccaagctt cgttttgcca agcaactagg ctttcttgaa
     9181 gcagatgaag atggttattt ccaaaaggct tttaaagagt taaaagtagc agagaatgac
     9241 tatctagaag tgactttgca ccctgtaaca aaagcctttc aggatctaat gtattcggca
     9301 gtggcttcat cagactatgc ccatcttttg gttatgctgg tcattgcaga aggtctctat
     9361 ttagactggg gttctaaaga tttggctcta cctgaagtct atattcattc ggaatggatc
     9421 aatctccaca gaggtccttt ctttgcagag tgggttcaat ttctggttga cgaactcaat
     9481 cgtgtcggta aaaatcgaga agatttgaca gaacttcagc aacgctggaa tcaagcagtc
     9541 gctttagaat tagctttttt tgatattggt tacaatgtct agagaaggct catgatattt
     9601 acaaatttga tgaggtaaga ctcttcgaaa atctcttcaa acaacgtcag tttcaccttg
     9661 ccgtaggtat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag
     9721 ctgacttcgt cagtcttatc tacaggttca aagcagtgtt ttgagcaacc tgcggttagc
     9781 ttcctagttt gctctttgat tttcattgag tataaatcat tcaatgtaaa tgaaaaaaaa
     9841 ttcttaacga aagatagaga taaatcgaaa tcagtagatc gtataaagtg aaaaggaagg
     9901 atttcaatat gacaagttta aaattattaa aagaaaaagc accattggtc atttgtataa
     9961 ccaatgatgt agtaaaaaat ttcacagcaa atggattagt agcactgggc gcctcaccag
    10021 ccatgagtga gtttccagca gatttagagg atttgttaaa gtatgctggt ggtttattaa
    10081 taaacatagg aacattgaca gatgaaaatt ggaaattata ccaagctgct ctgaaaattg
    10141 cggagaaata taatgtccca gcagttttag atcctgtagc ctgtggagca ggagaatata
    10201 gaaaaaaagt agcagatgat ctaattaata attacaaact agctgcgatt aggggaaatg
    10261 ctggcgagat tgcctcttta gtaggaatag atgtggcatc taaaggagta gatagtgcgg
    10321 gcgtagataa tattgacgaa attgctctag cagcaaatga gaagttcaat attccaatag
    10381 tagtaacagg tgaagtggat gccattgctg ttaatggaga agtggtaacg attcataatg
    10441 gtagtgccat gatgccaaaa gttattggaa ctggatgttt attaggggca gtggtagcaa
    10501 gctttatcgg actagaaaaa ggtcaagaat tgaaatcatt agaaacagca atgttagttt
    10561 acaatatcgc tggagaaatg gcagaaaaac gtcctaatgg acatcttcct ggagcattta
    10621 aagttgaatt tataaatgcc ttatatgaga ttacagatga agatgtaaag gaattcaaaa
    10681 gagtgaagta agatgtttca taaagaatta ctaagactat attttatttg tggaacgact
    10741 acttgccaag gaaaaaatct atatacagtc gttgaggaag ccttaaaagg tggtataacc
    10801 ttatttcaat ttcgagaaaa aggtgagggt gctttagaag gcttagaaaa acttgagcta
    10861 gctatccaaa ttaaagaact ttgtaaaaaa tacaatgttc catttatcgt taatgacgat
    10921 atagatttag caatggaaat tgacgccgat ggcgtacatg taggtcaaga tgatattggt
    10981 gttgatgaaa ttagaaaatt gatgccagat aaaataattg gtctttctat aagaaacgac
    11041 gaagaatttc aacaatcaaa agttgaatat gtagattatg taggtgttgg gcctgtattt
    11101 gatacccagt caaaagatga tgctggtggt gctataggtt atgaaggtct tgaattgatg
    11161 agaaaactat tgccacaaat gcctttagtt gcaattggtg ggatacagac gaaacatatt
    11221 aaagacatta taaagaccaa tatggatggc gtttcaatca tttcagcaat atcgtatgca
    11281 aaaaatatag aaaaaactgt tcgggaaatg agtgaacaat agatatctat ccttggattc
    11341 taagtaagta ggattttcct tttttcaaaa gctatgtcca gattcatctt gaatggctaa
    11401 ttcatcaaac aggtcatttg ttttttgcaa aagaaagatc tgctgtcttt gtgagtgcaa
    11461 ggctatattt ccagttcaat ttcttgaact agtataaaat gtgtgttata ataatcacat
    11521 aattaaataa taaagacatt tggggtgctt ta
//
LOCUS       DAVGJJ010000062        11353 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000062 DAVGJJ010000000
VERSION     DAVGJJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.062"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            120..797
                     /locus_tag="VVE35_001484"
     CDS             120..797
                     /locus_tag="VVE35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU3061653.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            784..1581
                     /locus_tag="VVE35_001485"
     CDS             784..1581
                     /locus_tag="VVE35_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU3061654.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQLFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLSQWKEEKGWTIDILPSQASTNPFHHI"
     gene            1598..2701
                     /locus_tag="VVE35_001486"
     CDS             1598..2701
                     /locus_tag="VVE35_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU3061655.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSKESDLEVTVFDHGQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALKRREESPLIGQLAILEQASANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            2708..2899
                     /locus_tag="VVE35_001487"
     CDS             2708..2899
                     /locus_tag="VVE35_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061656.1"
                     /translation="MKIKEQTRKLAADYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            3002..4021
                     /gene="galE"
                     /locus_tag="VVE35_001488"
     CDS             3002..4021
                     /gene="galE"
                     /locus_tag="VVE35_001488"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU3061657.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGIIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4023..4988
                     /locus_tag="VVE35_001489"
     CDS             4023..4988
                     /locus_tag="VVE35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3061658.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(5039..5245)
                     /locus_tag="VVE35_001490"
     CDS             complement(5039..5245)
                     /locus_tag="VVE35_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU3061659.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5297..5773
                     /locus_tag="VVE35_001491"
     CDS             5297..5773
                     /locus_tag="VVE35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU3061660.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            5782..6453
                     /locus_tag="VVE35_001492"
     CDS             5782..6453
                     /locus_tag="VVE35_001492"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU3061661.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            6584..6922
                     /locus_tag="VVE35_001493"
     CDS             6584..6922
                     /locus_tag="VVE35_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU3061662.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            6925..7554
                     /locus_tag="VVE35_001494"
     CDS             6925..7554
                     /locus_tag="VVE35_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU3061663.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            7795..8946
                     /locus_tag="VVE35_001495"
     CDS             7795..8946
                     /locus_tag="VVE35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3061664.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            9258..10007
                     /gene="truA"
                     /locus_tag="VVE35_001496"
     CDS             9258..10007
                     /gene="truA"
                     /locus_tag="VVE35_001496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU3061665.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            9997..10770
                     /locus_tag="VVE35_001497"
     CDS             9997..10770
                     /locus_tag="VVE35_001497"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU3061666.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            10748..11209
                     /locus_tag="VVE35_001498"
     CDS             10748..11209
                     /locus_tag="VVE35_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3061667.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         3205 a         2146 c         2645 g         3357 t
ORIGIN      
        1 ttcacttatt actttaggac attgaaactt ctttcgccag aggtgcttat attactggga
       61 agttgaagat tgaaatgtgg tataataaga ggtaatagag ttttggaaag tgagagaaga
      121 tgatttcaaa gagattagaa ttggtagctt cctttgtgtc acagggggct attttactag
      181 atgtgggaag tgaccatgct tatctgccta tcgagttggt tgagagaggc caaatcaaaa
      241 gcgctattgc aggtgaggtg gtggaaggtc cctatcagtc tgcggttaaa aatgttgagg
      301 ctcacggcct aaaggagaaa atccaagtcc gtttagccaa tggcttggca gcttttgaag
      361 agactgacca agtgtctgtc attaccattg ctggcatggg tggtcgtttg attgctagga
      421 ttttagaaga aggtttgggg aagttagcta atgtagagcg tttgatcctc cagcccaata
      481 atcgtgaaga cgacttgcgt atctggctac aggatcatgg attccagatt gtagcagaaa
      541 gcatcttaga agaagctgga aagttttatg agattttggt ggtggaagca ggacaaatga
      601 agctatcagc cagtgatgtt cgctttggtc ccttcttgtc caaagaagtc agtccagtat
      661 ttgtccaaaa atggcaaaaa gaagctgaga agctagagtt tgccctcgga caaatcccag
      721 aaaaaaatct ggaagaacgt caagttctag tagataagat tcaagctatc aaggaggtgc
      781 tccatgttag caagtgaagt gatccaagct tatgaagcct tttgccctca ggaattttct
      841 atggagggag acagccgtgg tctgcaaatt ggcactttag acaagggtat ccaaagggtc
      901 atggttgccc tcgatattcg tgaagagaca gtggcagagg ccattgaaaa gggtgtggac
      961 ttgattatcg tcaagcacgc gcctatcttt cgtcctatca aggacttgct tgccagtcgt
     1021 ccgcaaaatc agatttacat cgacctgatt aagcatgaca tcgcagttta tgtcagccat
     1081 accaatattg atatcgttga aaatgggctc aatgactggt tttgtcagat gctaggaatc
     1141 gaggagacga cttatctgca ggaaacaggt ccagaacgtg gaattggacg tattgggaat
     1201 attcagcctc agacattttg ggaattggcc caacaggtca agcaagtctt tgacctagat
     1261 agccttcgaa tggtgcatta tcaagaggat gatttgcaga agcctatttc aagagtagca
     1321 atttgtggtg gaagtgggca gttattctat aaggatgctt tggcaaaggg ggcagatgtc
     1381 tatatcactg gcgatatcta ctaccatact gctcaggata tgctgtctga tggcttgctg
     1441 gcattggacc caggtcacta tattgaagtg ctttttgtgg aaaaaattgc agcactcctt
     1501 agccaatgga aagaagagaa gggctggact attgatatct tgcctagtca agcatcgacc
     1561 aatcctttcc accatatcta gttagaaagt gaaaacaatg aaaaaagttg ccattatcgg
     1621 agcaggaatt gtgggagcaa cagctgccta ctacctctcg aaagaaagtg acctagaagt
     1681 aaccgtcttt gaccatggac aaggtcaggc taccaaggcc gcagcaggaa ttatcagccc
     1741 ttggttttcc aaacgccgca ataaggcctg gtacaaaatg gcgcgcttgg gggctgactt
     1801 ttatgtggat ttattagctg atttagagaa atcaggacaa gaaatcgact tttaccagcg
     1861 ttcgggagtt tttctcttga aaaaggatga atccaagttg gaagaactct atcaactagc
     1921 tcttaagcgc agggaagaat ctcccttgat aggccagtta gccattctgg aacaagcatc
     1981 agctaatgaa ttattccctg gcttgcaggg attcgacaac ttgctctatg cttctggtgg
     2041 agcgagagta gatggtcacc tcttagtgac tcgtttgctg gaagccagtc aggtcaagtt
     2101 ggtcaaagaa aaagtgactc tgacactctt atcatcaggc taccagattg gcgaagaggt
     2161 gtttgatcag gttattttgg caacgggagc ttggttgggg gacatgttag agcctttagg
     2221 ttatgaagtg gatgtccgtc ctcaaaaagg acaactacga gattatcagc ttgcccaaga
     2281 catggaagat taccctgttg tcatgccaga aggggagtgg gatttgattc cctttgcagg
     2341 tgggaaatta tccttgggcg ctacccatga aaatgacatg ggatttgatt tgacggtaga
     2401 tgaacccttg ctccaacaaa tggaggaggc ggccttgcct cattacccag ttttagctag
     2461 agcgacttca agggctgagc gtgtgggaat ccgtgcctat accagtgatt tctctccctt
     2521 ctttgggcag gtgccagaat tggcaggtgt ctatgcagcc agtggactag gttcatcagg
     2581 cctcacaact ggtcctatca ttggttacca tctagcccaa ctgatccaag acaaggagtt
     2641 gaccttggac cctgaaaact acccaattga aaactatgtc aaacgagtaa aaagcgaata
     2701 atactcaatg aaaatcaaag aacaaactag gaaactagcc gcggattact caaagcactg
     2761 ctttgaggtt gtggatagaa ctgacgaagt cagctcaaaa cactgttttg aggttgtaga
     2821 tagaactgac gaagtcagta accataccca tggcaaggcg acgttgacgt ggtttgaatt
     2881 ggattttcga agagtataag cattttactg aaattttatc aggtagttta ggatggcaaa
     2941 tgacattccc tgtcaaaaat ggtaaaataa gaaaaaataa tctgagaatc gaggaaaaaa
     3001 gatgcaagaa aagattttgg taactggtgg tgccggtttt attggaaccc acactgttat
     3061 tgaattgatc caagcaggcc atcaagttgt tgtggtggat aatcttgtca atagcaatcg
     3121 taagagttta gaagttgttg aaagaatcac aggagttgag attcctttct atgaggcaga
     3181 tattcgtgac acagataccc tcagagatat tttcaagcag gaagaaccga ctggtatcat
     3241 tcactttgct ggtttgaagg ctgttggcga atcaacacgt atccctcttg cctactatga
     3301 caacaatatc gctggaactg tcagcctttt gaaagccatg gaagaaaaca actgtaaaaa
     3361 catcatcttc agttcttctg cgacagttta cggggatccg cacacagtgc ccatcttgga
     3421 agatttccca ctttcagtga ccaacccata cggtcgtact aagctcatgc tagaggaaat
     3481 tttgactgat atttacaaag cagactcaga atggaatgtt gtcttgcttc gttactttaa
     3541 cccaatcgga gcccatgaga gtggtgattt gggagaaaat ccaaacggta ttccaaacaa
     3601 tctcttgcca tatgtgactc aagtagccgt tggaaaatta gagcaagtgc aagtgtttgg
     3661 agacgattac gatacggaag atggaacagg tgttcgtgac tatatccacg ttgtcgattt
     3721 ggctaagggt cacgttgcag ctttgaaaaa aatccaaaaa ggttcaggac taaacgttta
     3781 taaccttgga actggtaaag gttactcagt tcttgaaatt atccaaaaca tggaaaaagc
     3841 ggtgggacgt cctattcctt accgcatcgt agaacgtcgc ccaggtgata tcgctgcctg
     3901 ctactcagac ccagcaaaag ctaaagcaga actcggttgg gaagcagaac tcgacatcac
     3961 ccaaatgtgt gaagacgcat ggcgttggca aagcaagcat ccaaatggat ttgaagacta
     4021 agatgatgat ttcaatcatc gtcccttgtt taaacgaaga ggaagtactt cctctttttt
     4081 atcaggctct ggaagcttta cttccagatt tggaaacaga aatcgagtat gtctttgtcg
     4141 atgatggatc aagtgatggg accttggaac tcttaaaggc ctatcgggag caaaatccgg
     4201 cagtccatta tatttctttc tctcgaaatt ttggcaaaga agcagccctt tatgcaggct
     4261 tgcaatatgc gacaggagat ttggtggtgg tgatggatgc agacctccaa gatcctccta
     4321 gtatgttgtt tgagatgaaa aatgtactag acaaaaatgt agacttggac tgcgttggga
     4381 cacggagaac tagtcgggag ggagaaccct tctttcgcag tttctgtgct gttctctttt
     4441 atcgcctcat gcaaaaaatc agcccagtag ctctgccgtc gggtgtccgt gattttcgta
     4501 tgatgagaag gtctgtggtc gatgccattt taagtttgac tgagtccaat cgtttttcta
     4561 agggactctt tgcctgggtc ggctttaaaa cccactatct ggactatcca aatgtcgaaa
     4621 ggcaggctgg caagaccagt tggagtttta ggcaactctt tttctactcc attgaaggga
     4681 ttattaattt ttcagatttt cccttgatta tagcctttgt agctggtctc ctatcttgtt
     4741 ttctttctct gctgatgacc ttttttgttg tggttcggac cctcattttg ggcaatccga
     4801 catcgggttg gacctctctg atggctgtca ttctctttct tggaggcatt caactcttga
     4861 ccattgggat tctcggcaag tatatcagta agatttattt agagactaaa aaaagaccac
     4921 tttatcttat caaagaaaaa agtgaccttc ctgattttac agaaaaaaat aaagtgaaaa
     4981 gactataatt ttacatggaa atgtgctaaa ctagagggag tggattatcg ccattgattt
     5041 aggcttcctg gtttccaatc agggcgcgag ttgggcaatt tttaacagcc tctaagacat
     5101 cctgactagg agaaatttct ttttccagtt ggtcagggtc atcgtaaaaa cgcacgattc
     5161 cattatcgtg gtaatcaaat aaatcagaat aagtttggca aagcccacag gcgatacatc
     5221 gttcaggtat aagtgtgatt ttcatgttca tatttatatt gtaataagaa aagtaaaaaa
     5281 aaacaaggag taaggtatgg caaaagaacc gtggcaagaa gatatctatg atcaagaaga
     5341 atcaagagca gagcgtcggc atcgaaacca cggaggggct gataggatgg ctaatcgtat
     5401 tttgacgatc ctagctagta ttttctttgt aattgtggtg gtgatggtca tcgttctcat
     5461 ctatctatca tcggggggaa gtaatcgcac agcagcctta aaagactttc atgattctga
     5521 tgcaagtgta gtacaaatct catcttcaag cagttctcag cctgagcaga gttcagagcc
     5581 agaatctact tctagtagtt cagaagaagc tgctaatcct gaaggaacga ttaaagttct
     5641 cgcaggagaa ggggaagcag ctattgccgc tcgtgcagga atctccattg ctcagttaga
     5701 ggccttgaat cctgggcaca tggctacagg atcttggttt gctaatccag gtgatgttat
     5761 aaaaataaaa taggagccga tatgaaaaca attcaaattg ctattgatgg tcctgcttcc
     5821 agcggtaaga gtacggtcgc aaagattatt gctaaggatt ttggattcac ctaccttgat
     5881 acaggagcta tgtatcgtgc agcgacctat atggctctta agaaccaatt aggagttgaa
     5941 gaagtcgaag cccttctagc cttgttggac cagcatccaa tcagctttgg acgttcagaa
     6001 actggagacc agcttgtttt tgtaggagat gtggatatta cccatcctat ccgtgaaaat
     6061 gaagtgacca atcatgtttc tgctattgca gcaattcctg aagtgcgtga gaaactggtt
     6121 tctctccaac aagaaattgc ccagcaaggc gggattgtca tggatggtcg cgatattgga
     6181 actgttgtat tgccacaagc agaattgaaa attttcctag tagcttctgt tgatgagaga
     6241 gcagagcgtc gttacaagga aaatattgcc aagggaattg aaacagacct tgaaacccta
     6301 aaaaaggaaa ttgctgcgcg tgactacaag gatagtcatc gtgagacttc tcctctcaaa
     6361 caagcagagg atgctgtcta ccttgataca actggtttga acattcaaga agtagttgaa
     6421 aaaatcaaag cagaagctga aaaaagaatg taaatgtaga aaagccgata gattgatatc
     6481 ggtttttttc taaatttgtc aaaatgctga ttttagggta taatagttgc tgttcgagaa
     6541 attttgattt tcaaagaata gtgaatgatg ataaggagaa accatgaaca atttaccaaa
     6601 ttgtccaaaa tgtaactcag agtatgtcta cgaagacggt gccctactgg tttgcccaga
     6661 gtgtgctcat gagtggaatc ctgctgaagt tgcagaagta gaagaaggtc ttgtcgctat
     6721 cgatgccaac ggaaataaat tgactgatgg tgatacagta actctcatca aggacttgaa
     6781 agtaaaaggt gcgccaaaag atttgaaaca agggacgcgc gtgaaaaata tccgcatcgt
     6841 agaaggcgat cacaatatcg actgtaagat tgatggtttt ggtgccatga aacttaaatc
     6901 agagtttgtg aggaagattt aagcatgtca aaacactata aacttgtatt ttatagccgt
     6961 atcttcttgt ttctagcggc ttttacggga gtttatcttg aaattactag gcatggtggt
     7021 tttgggatgc ttctctatta cacggttctg tccaacctct tggtaactat ttttaccctt
     7081 tatcttctaa aggttatgag ccgtgtaggt gaaaattggc aaaggccaag tctcttgcgc
     7141 ttaaaaggtg gggtcaccat gagtatcatg attacctgtg tgatttacca tttcctcttg
     7201 gcgcccattg cgactaattt ctatacccta gaaaatttcc tttgccacta tatcgttccc
     7261 atctggtttt tagcggatac cctctttttt gacaaacagg gtcaatacaa gatttgggat
     7321 ccagcagtgt ggaccatttt accctttctg tatatgatgt ttgctctttt taatggcttg
     7381 gttctaaaac tcaatattcc aaatgccaag gataatcctt tcccttactt ctttttgaat
     7441 gtgaacaagg gttggaatgt tgtgtttaag tggtgtctga ttatctttgt tgcctatatg
     7501 gtagcaggat ttattttcta ctttatcaag caaatcaaga gaaagtcatc ctaatactct
     7561 tcgaaaatct cttcaaacca cgtcagtttc catctgcaac ctcaaaacac tgttttgagc
     7621 agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag atacaaggga
     7681 ttcattttcg aatttaagaa ggagtctaca aaccaatgag gcttcttctt ttcttgctta
     7741 tttgataact atcaaaaaaa gtagaagatt aagagaaata tagaaagaga tttcatgaaa
     7801 caatttttag aacgggccag cattttggct ctctccctcg ttttgattac ctccttttcc
     7861 atttcgagtg ccctaccagc catgtttgac tattatcagg gttattctaa ggaacaaatt
     7921 gagctcttgg tgagcttgcc ttcctttgga atcatgatga tgttactgct aaatggtttc
     7981 ttagaaaaaa tatttcctga gcgcttacag attagtttgg gcttgctgat tttatcattg
     8041 agcggtacag ctcccttctg gtaccaagcc tatccctttg tctttggaac acggcttctc
     8101 tttggtttgg gtcttgggat gatcaatgcc aaggccattt ctattatcag tgaacgctac
     8161 caaggaaaaa ggcgaattca gatgttaggg ctacgcgctt ctgcagaggt cgttggagct
     8221 tctctcatta ccttggccgt cggtcagttg ttggcctttg gttggacagc tatctttcta
     8281 gcctatagtg ctggattttt ggtgctgccc ctttatctgc tctttgtccc ttatggaaaa
     8341 tcaaagaaag aagtcaagaa aagagcgaag gaagcaagtc gtttaactcg agaaatgaaa
     8401 ggcttgattt ttaccttagc tatcgaagcg gcagttgtag tttgtaccaa tacagctatt
     8461 accatccgta ttccaagttt gatggtggaa agaggattgg gggatgccca gttatctagt
     8521 tttgttctta gtatcatgca gttgatcggg attgtggctg gggtgagttt ttctttcttg
     8581 atttctatct ttaaagagaa actgctcctc tggtctggta ttacctttgg cttggggcaa
     8641 atcgtgattg ccttgtcttc atccttgtgg gtggtagtag caggaagtgt tctggctgga
     8701 tttgcctata gtgtagtctt gacgacggtc tttcaacttg tctctgaacg aattccagct
     8761 aaactcctca atcaagcaac ttcatttgct gtattaggct gtagtttcgg agcctttacg
     8821 accccattcg ttctaggtgc aattggctta ctaactcaca atgggatgtt ggtctttagt
     8881 atcttaggag gttggttgat tgtaatctct atctttgtca tgtacctact tcagaagaga
     8941 gcttaggatt gattcctaag tttttctttt gatactcaat gaaaatcaaa gagcatagtt
     9001 gattgagttt ggaatagtac gctgtagctg ctaaaatatt tctagaaatt aatttgactt
     9061 tcctaataga gttgttcata tcttatttca atctactata gaaagctagc cgcaggttgc
     9121 tcaaaatagt gttttgaggt tgcagatgga aactgacgtg gtttgaagag attttcaaga
     9181 gtataagatt tttataccca tacaatattt gttttaggtc cttgtttaca gactaatttc
     9241 ttgataaaat agaggttatg acgagatata aagcaactat ttcctatgat ggttatgcct
     9301 ttgctggctt tcagcgccag cctcatgcgc gtagcgttca ggaagaaatt gaaaaaacct
     9361 tgaccagatt aaataaaggg caaaccatta ctgttcacgg tgctggtagg acagatagtg
     9421 gggttcatgc cctgggacag gtcattcatt ttgacctgcc ttatcagatg gatgaggaga
     9481 aactccgttt tgccttggat acccagtctc ctgaagatat tgatgtgatt tcgattgagc
     9541 ttgtggcagc tgattttcat tgccgttatg ccaagcatag caagacctat gagtttattg
     9601 tggatagagg acgtcccaaa aatccgatgc gccgtcacta tgccacccac tttccctacc
     9661 cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct agagggaacc catgatttta
     9721 ccggttttac agcctctggg actagtgtag aggataaggt tcgcaccatc acagaagcta
     9781 gtttaatagt cgatgagaca ggacaatttt tgacctttac cttttcagga aatggtttct
     9841 tgtataaaca gattcgcaat atggtgggga ctcttctcaa aatcggtaac aaccgcatgc
     9901 cagtagagca gattgatctc atcttggaga agaaggacag gcaacttgca ggtcccactg
     9961 cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga agaataatcg tattttagca
    10021 ctttctggaa atgatatttt tagtggtggt ggactgtcag ctgatttggc tacctatacc
    10081 ttgaacggct tgcatgggtt tgtagcagtg acttgtttga cagccttgac agaaaaagga
    10141 tttgaagtct ttccaactga tgataccatt tttcaacatg aattagatag cttgcgtgat
    10201 gtggaatttg ggggaattaa gattggtctt ctccctactg tcagtgtggc tgagaaggcc
    10261 ttggacttta tcaaacaacg cccaggagta cctgtggtgt tggatcctgt cttggtctgc
    10321 aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag agttgattcg ctttttccct
    10381 tatgtcagtg tgattacgcc taatctccca gaagcagaat tattatccgg tcaggaaatt
    10441 aaaaccttgg aagacatgaa aactgcagcg cagaaattgc atgatttagg agcgccagca
    10501 gtcattatca agggaggcaa tcgtcttagt caggacaagg ctgtggatgt cttttatgat
    10561 ggacagacct ttactatcct agaaaatcca gttatccaag gccaaaatgc tggtgcaggt
    10621 tgtacctttg cctctagcat tgccagtcac ttgattaaag gtgataaact tttgccagca
    10681 gtagaaagct ctaaggcttt cgtttatcgt gctattgcac aagcagatca gtatggagta
    10741 agacaatatg aagcaaacaa aaacaactaa aatcgccctt gtatccctat taaccgccct
    10801 ttctgtggtt ctaggttatt tcttaaaaat cccaacacct acaggaattc taactctttt
    10861 agatgctggt gtcttctttg cggcctttta ctttggtagt cgtgaaggag cggtagtcgg
    10921 aggactagca agtttcttga ttgacctctt atcaggctac cctcagtgga tgttctttag
    10981 cttggtcaac catggcttgc agggattttt cgcaggattt aaaggaaaaa gtcagtggtt
    11041 aggccttatt ttagcaacta ttgccatggt aggaggctac gccttgggtt ctactttgat
    11101 gaatggctgg gcagcagccc tcccagaaat tctaccaaat ttcatgcaaa atatggtagg
    11161 gatgattgta ggatttattc ttagtcaaag tatcaagaag attaagtaat actcttcgaa
    11221 aatctcttca aaccacgtca gcttccatct gcaacctcaa accactgttt tgagcagcct
    11281 gaggctagct ttctagtttg cactttgatt ttcattgagt ataaagaggt tgagtaaaaa
    11341 gtatttcaaa taa
//
LOCUS       DAVGJJ010000063        11319 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000063 DAVGJJ010000000
VERSION     DAVGJJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.063"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(127..1263)
                     /gene="dnaJ"
                     /locus_tag="VVE35_001499"
     CDS             complement(127..1263)
                     /gene="dnaJ"
                     /locus_tag="VVE35_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU3061668.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPET
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1468..1821)
                     /locus_tag="VVE35_001500"
     CDS             complement(1468..1821)
                     /locus_tag="VVE35_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061669.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(1834..3657)
                     /gene="dnaK"
                     /locus_tag="VVE35_001501"
     CDS             complement(1834..3657)
                     /gene="dnaK"
                     /locus_tag="VVE35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU3061670.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4137..4661)
                     /gene="grpE"
                     /locus_tag="VVE35_001502"
     CDS             complement(4137..4661)
                     /gene="grpE"
                     /locus_tag="VVE35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU3061671.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4688..5722)
                     /gene="hrcA"
                     /locus_tag="VVE35_001503"
     CDS             complement(4688..5722)
                     /gene="hrcA"
                     /locus_tag="VVE35_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU3061672.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5886..6395)
                     /locus_tag="VVE35_001504"
     CDS             complement(5886..6395)
                     /locus_tag="VVE35_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU3061673.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7002..9335
                     /locus_tag="VVE35_001505"
     CDS             7002..9335
                     /locus_tag="VVE35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU3061674.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9348..10811
                     /locus_tag="VVE35_001506"
     CDS             9348..10811
                     /locus_tag="VVE35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU3061675.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            10811..>11319
                     /locus_tag="VVE35_001507"
     CDS             10811..>11319
                     /locus_tag="VVE35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3061676.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPY"
BASE COUNT         3467 a         2187 c         2224 g         3441 t
ORIGIN      
        1 aaatcaaaaa gcaaactagg aaactagcca cgggctgctc aaaacactgt tttgaggttg
       61 tagatacgac tcacatatat acggcaaggc aacgctgacg tggtttgaag agattttcga
      121 agagtattat tctccatcaa aggcatcttt tatatggtca aagaagcctt ttttctttgg
      181 atttactttc aagtcaccag cagtcgcgaa ttctttcaag gctacttttt ggcggtcgtt
      241 caagcctgtc ggtgttacga cattaacagt aacgtattgg tcaccaactg caccgccacg
      301 aaggctcggt gcccccttac tacgtaggcg gaatttctta ccagtctgag ttccctctgg
      361 aataaccaat tcaacatcac cgtgaacagt tggaatatct actgtatcac caagagccgc
      421 ttggacaaag ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc
      481 gctagcttct acagaaacta ctacatacaa gtcaccataa ggtccaccgt taaagcctgc
      541 ttcaccttga ccagcgaggc gaatttgttg acctgtttcc acaccagcag ggattttcac
      601 atgtacgcta tgagcttgtt tctcatgacc tgttccatga caggttgtac atggatattt
      661 gatttctttt cctcgaccgt gacagacatc acaggttact tggcgacgca tcataccaag
      721 aggagtctgc gtatcgacgt taatgacacc agcgccatga cagcgtccac aagtgactgg
      781 acttgtctct ggcttagcac cagagccatt acatgtacga cagccagctt cacgatgata
      841 cttaacttcc ttctcagttc cgaagatagc ttcttcaaat gtcaaattga cacgatactg
      901 gagatcatct ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa
      961 acttgagaaa atatcctcaa aaccaccgaa gccacctgcc ccattgaaac caccgaaacc
     1021 accagctcca ccaaaaccac cattggcgcc tgcagcacca tactggtcat aggcagcacg
     1081 tttttggtcg tcactcaaag tctcataggc ttcttgaact tccttgtact tgtcctcagc
     1141 accaggctcc ttgttgatat ctgggtgata ttttttggaa agcttacgat aagccttttt
     1201 gatctcgtct gccgaagcgt tttttgacac ccccagacga tcataaaatt cagtattgtt
     1261 catacaagat accaagaccg taaaattcga ctgaaaaata ggaaatctga cgctggagcg
     1321 acgctcctag acagatttct cttttttccg agaatttagg tcgggttcaa ttcctttctt
     1381 ttatattgat gagataatac tccagccact cggccctatc cctttttacc gaaaaacaga
     1441 ggctgggttg caacctctgt ggttagatta ttcatttgaa ataaaaatgg ttgtattatc
     1501 tctagtagta atcttcaaag accttttagc taaatcaaac tcaaatttat tatagtgtgg
     1561 gtcttgtgtt ttatataggt gtaactcctg acatatatga tccgaaaagt aaatcataac
     1621 atctttaaac acagaagtgt agtcatattt actcttacca acagctactt ctgtaaattc
     1681 tttataaatg acatttccat ttctttccac agtcaccagc atttctgttg gattataatc
     1741 caaattaaat aattcttcaa aaaactttat cttttcatga taatctgcaa tgtcagaaag
     1801 attattttta ttaacactca taacactagc cccttatttt tcagtaaact ctccgtctac
     1861 gacgtcatcg cctgcgtttc ctgttgcttg tgcgccttct gctccttctt gagcttgttg
     1921 cgctgctgcg gcttgttcgt agagtttaac agcaagtcct tgagcttttt cgttcaatgc
     1981 ttcaagtttc gctttcatgt cgtccaagtt gttgtcttct tgagctttct taaggtcatc
     2041 aagggcagct tgggcagcgt cacgttctgc gtcgaagcct ttaccttcag tttccttgat
     2101 tgtcttttca gtcgcaaaga ttgcttggtc tacttcatta cgaaggtcta cttcttcttt
     2161 acgtttctta tcggcttcag cgtttgcttc tgcatctttc atcatgcggt cgatttcttc
     2221 gtcagtcaaa cctgagttcg attggatgac aatagtttgt tctttttgag ttccaaggtc
     2281 tttggcctta acagacacga taccgttctt gtcgatgtca aatgttactt cgatttgagg
     2341 aattccacga ggtgcagctg ggatatcagt caattggaag cgtccaagag tcttgttatc
     2401 tgctgccatt gggcgttcac cttgaagaac gtggatatca acggctggtt ggttgtctgc
     2461 tgctgttgag aagacttgtg atttagatgt tgggattgta gtgttgcgat cgataagttt
     2521 tgtaaatact ccacccattg tttcgatacc aagtgacaat ggcgttacat caagaaggac
     2581 aacgtccttg acatcaccag taatcacacc accttggata gccgcaccca tagcaactac
     2641 ttcatcaggg tttactgatt tgtttggttc tttaccagtt tcagctttaa cagcttcaac
     2701 aacggcaggg atacgagttg aaccaccaac aaggataact tcgtcgattt ctgacaagct
     2761 caaacctgca tctgaaaggg cttgacgaac tggaactttt gtacgttcaa caaggtcacg
     2821 agtcaaatca tcaaatttcg cacgagtcaa agtcatttcc aagtgaagag gtccagcctc
     2881 acctgcagtg ataaatggca agctgatttg tgttgaagtt acaccagaaa ggtctttctt
     2941 cgctttttca gccgcatctt tcaaacgttg cattgccatc ttgtcagtag acaagtcgat
     3001 accgttttct ttcttgaatt ctgctaccaa gtggtcaatg attttttggt caaagtcgtc
     3061 accaccaagt ttgttgtccc ctgcagttga caatacgtcg aagacaccgt cacccaattc
     3121 aaggatagag acgtcgaatg taccaccacc aaggtcaaat accaagattt tttcttcttt
     3181 atcagtcttg tccaaaccat aagcaagagc tgctgcagtt ggttcgttaa caatacgttc
     3241 tacttcaaga ccagcaattt taccagcgtc ttttgttgct tgacgttgag cgtcgttgaa
     3301 gtaagccgga actgtgataa cagctttggt tactttctca ccaaggtagt cttcagcgta
     3361 gcctttcaag tattgaagga tcatagctga gatttcttgt ggagtgtatt cttttccatt
     3421 tgcagaaact ttttcagaag ttcccatctt agatttgata gagataactg tatctgggtt
     3481 tgtgactgct tgacgttttg cagcatcacc aacgatgatt tctccgtttt tgaatgagac
     3541 tacagatgga gttgtgcggt ttccttctgg gtttgcgatg attttgcttt cagttccttc
     3601 aagaactgca actgctgagt ttgttgtacc taagtcaata ccgataattt tagacatgtg
     3661 tttttctcct tgttagttaa ttttctattt ttctttttga tactcttctt aagtggcgac
     3721 gccgtcagag cttgacttcg tcaatctctt tgactaaact ttgagcctaa ggtctcaaaa
     3781 tttgcgcaaa tagcgccacg gcgaagagta tcgtgtttgg ttcgcttcgc tcactattac
     3841 aaagccaaac atattttatc tttcttcata gtttattgtc gtttcggaca agttttctat
     3901 tatgtaaatt gcgacacgaa gagtcgaaat cgattttatt tcgacgacga gttagtaagg
     3961 aagctaggca aacgccatag cgattgccgt tttctgacga gttgctttag ctaccgtcag
     4021 aattgccgaa tcgaacacgc cctaagctct gagtaaaaaa gatagatttt cttgtgttca
     4081 tcgaacactt cgttaatctc ctatttttac tgcgagcttt ttacgggctt tgtatcttag
     4141 ttatacacca ctaccattgc tgggcgtagg atgcggtcat ggagtttgta gcctttttga
     4201 aagacttgag cgatggtatc tactgggtgt tcatcgtctg ctgggagagt ttggatggcc
     4261 atatggtagt tatggtcaaa ttcgccatct gctgcgattt cttcaattcc ttcttctttc
     4321 aaagcgtgaa tcaagctttc ttgcaccatc cccaagccct tcttcacatc atctgtcaaa
     4381 ccttcaactg caagtgcacg ctcaaggttg tcaagagatg gtaagattgc ttttgccaag
     4441 tcctggctac gataacgttg caagttttga cgttcttcat tggcacggcg ttggatattt
     4501 tgcatttctg catgagcgcg aagatatttg ttttcgaact catctgcacg ttcatttgcc
     4561 aagtccaact cagacttttc aggagttgtt tcttcagctg tttccacaac ttcctcttct
     4621 tgaacttctt ctacttcttc attttttata tcttgggcca tgttcgcctc ctttaatgat
     4681 ttcaatctta atgtacttcg taatgattac tgctgaggta gcggtaaaaa tctgtcaact
     4741 tcatggtcaa aacacggttg accacattga tttgattgat tagctgttgg taatccagat
     4801 taactggacc gataatggct agaattccaa ctccccgata aggaatgagg aacttactac
     4861 taatcaccgc taggtcagct aaacaggact cttgaccgtc tgcaatacga acattttgca
     4921 tctgatcctc acgcaacccc tcacgaatct ccaaggccac cttttgcggt tggtcaaaga
     4981 actgataggc tgctagattg gcaaaattca agagattgac cttgcccgcc atcacaatgt
     5041 tttcgttgaa catttcctta aagatgtgtt caaagagatc gatgacatta tccgttgttg
     5101 taaagtaacg ctggataatc tgcggaatct ccgtccgaat cttgtagtga atatctaaaa
     5161 cggtgtgacc gaggaaacgt tcctgaatga tgctcttcag tttcagcaaa tcctcctgca
     5221 agaagttcct tggaatcaga aactgactag taaccgttcg cgactcgtct agggtaaata
     5281 ccgccaaggc tgtatgttgc cccaaaacaa cgatatcaaa ggctgtcaaa cgttgcctgc
     5341 tcggctcaac atccagtgcc actaccgtac agccactcag gtctgttagc aagttagcag
     5401 cctcttgcag aatatcctcc aatttgaaga attcctgatc aaaggctttg acaatctcat
     5461 atacctcatt ttcagccagc cggtgaaaat ccagtgagtg tttcacatag tactgaaaac
     5521 cagcaacact tggcatccga ccgcttgaag tatgagcctt ctcaagcaac ccttgttttt
     5581 ctagttccgc catgtcatta cgaatggttg cactgctaga gttaatagac tcttgcaagg
     5641 cttttgatcc gacaggttcg tgcgttttgg taaagatgtc aataatcaga tttaaaatat
     5701 cctgctgacg ctctgtaacc atctcatcac tcctctctgt ctgatcaatt agcactcaat
     5761 gcatccaagt gctaacttat gattctatta tacaccctta gaagagaatg tcaagacaaa
     5821 aactcaaaaa attagcactc ttttatcaag agtgctaaaa atcaatctga agacctagaa
     5881 ccttgctatt catctactcg gtatttcctt tttagtctga aataaccatg aattagataa
     5941 gaaatcagaa aagcatataa aacaaaaata agaaagaaag acatagaaag atcctctcta
     6001 aacaaactcg tacaaacaac aagaccacct aagaaagtag ttgtacagga acgaagtcta
     6061 gatcgcataa cttcccattt gagcccctta ctcatataga cttgatcctc tggaagtaaa
     6121 tcagaagacg atttacgaag aatcgaacaa gaacctgctc ctatcaattc ccaacctcta
     6181 tctctaaaat cttgcagttc atgcttatat tttttaagaa atctagaatc atagatacgg
     6241 tagatgacat cgtctggttg acattggtca aaatagaaca aaccaaaacg actcgttcta
     6301 tacctccaac ctttcaaatg catctcatgt aaatattctt cttccttgtc caaatcaaca
     6361 atagtgaaaa tccgaaattc tactctgcta ttcattgtct taccccaaaa ttagaaaaca
     6421 tgcctggcgt tatttattag ataattcttt ccgcttttga ctcaatctcc aaaaaatata
     6481 agaaatctga atcgcaaaaa ctatcaataa aacccaatct attatgaaag tcaaaaacac
     6541 ttcccaactg aaagaactac ctccagtgac aaactttgag aaaaacggta gtagagctaa
     6601 aaagagaagc aaaataggaa gcatccgcat tgttaaaatc cttttggcat aaaaaaatct
     6661 ttatttaaac gaaaatatta tggcaaaatt tacgccagtt tttgaacggc tgatgtagat
     6721 attttatact ttcaaaatgt ttaaatgtga ttatttattt ttgaaaaata gctcaccagc
     6781 ccgactgaaa gtgcttataa atgataataa gtcgcctgcc gaaaacagcg aaaaatagcg
     6841 gtgttatgcg gagataatct gacgcgatgc gaaagtatat tgcatactta ttttcaacaa
     6901 tttagcagag tatttttata agtgtgatat aatagaagta taatttgttc tgatagttta
     6961 ttttatggag aagtagattt ttagaatgcg gagggttcaa tatggttgag tttataaagt
     7021 ctaagaaaga aatgagtgag gaggatatta aagcaaattt catcactcct gctattgtat
     7081 ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga atacttcact gatggtcgaa
     7141 ttgaggttag aggagataag gctcgtcgta aagaaggaaa aaaatcagac tattcactgt
     7201 attaccaatt tggaactcga attgcaattg ttgaggcaaa ggataataaa cacagcgttc
     7261 gagcaggatt acaacaagct attgaatatg gagagatttt agatgttcca tttgtttatt
     7321 cttcgaatgg tgatggcttt attgaacacg accgtatcac gagagaagaa cgtgagctgg
     7381 agttagacga attccctact cgtgaagaat tattttctcg tatgacgaag gaaaaaggat
     7441 tgacgtacga aattacagaa gctatctcaa ctccatacta tacagacgcc ttctcaatga
     7501 aaacgccacg ctattatcag caaatagcta tcaaccgtac tattgaaaca gttgccagag
     7561 gacaaaaacg agtaatgttt gtgatggcaa caggaacggg gaaaacgttc atggcttttc
     7621 aaattattca tcgccttcga aaagctggtt tggctaaacg agttttattc ttagcggata
     7681 gaaacatctt agtagaccaa acgatggctg aagactttag gccattcgaa aaggtaatga
     7741 cgaaaattac accaaaactt ttgactgctc ctgaaaaatt aaattctttt gaaatttatc
     7801 tagggcttta tcagcaacta actggtgaag atggaactga aacacattat caaaaatttg
     7861 acaaagactt ctttgattta atcgtaattg atgaagcgca ccgtggttca gctaaggaaa
     7921 acagtaactg gcgtaagata attgattatt tcagttctgc gacacagatt gggatgaccg
     7981 ctactcctaa agaaaccaag aatgcttcca atacggaata ctttggtgag ccaatctata
     8041 cttatagttt aaaacaggga atcgaggatg gttttttggc tccatatcgt gttatgagag
     8101 ttaatttaga tgtagatgtg gatggttatc gtccagaaac tggaaaagtt gatgctaacg
     8161 gacaattaat agaagatagg tactacggca ggaaagattt tgataaaacc attgtcattg
     8221 atgatagaac gcaaagagtt gccaagtttg tttctgatta tatgaagcaa aacaatgcac
     8281 gatttgataa aacaattgtt ttttgtgttg atattgacca tgccgagcga atgcgtgctg
     8341 cacttgtaaa agagaatcta gacttagtcc aagaagacta tcgttatgtc atgcaagtaa
     8401 ctggtgacaa cgctgaagga aaagctcaac tggataactt tatggatgtc aattctaatt
     8461 ttcccgctat tgtaacaacg tctaaattat taacgacagg agttaatgct aaaacatgtc
     8521 gtttgattgt tttagactct aatatccaat ccatgactga atttaaacaa attattggtc
     8581 gtggcacacg tctttatcct caaaagggga aagaattttt tacgattatt gattttcgaa
     8641 atgttaccaa tttgtttgct gaccctgatt ttgatggtga gccagtgaag gtgctggaaa
     8701 caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt agatgaggaa ggtgacccag
     8761 tagaaaaata tatcgttaca gacaagcagg ttaccattct taattctact gttcaagtat
     8821 tggatgaaaa cgggaaactg attaccgaaa gcctgaccga ctacactcgg aagaatatct
     8881 taggtagcta cgccactttg aacgatttta tcacagtttg gcatacggcg gataaaaaga
     8941 agcttatctt agacgaactt tataaaaaag gagtttatct agatgctatt cgagagtcgg
     9001 agggaatatc agaacaagaa atcgatgatt ttgatttact tctaaaactt gcctatggtc
     9061 aaaaagaatt aaccaaaacg gaacgtatca ataaactcaa acaaagcgga tatttatata
     9121 aatatagtga ggaagcgcgt gctgttttgg aaattttact gaacaaatac atggataaag
     9181 gtattggaga actcgaaagc attgaaacat taaaacttcc agaatttcag atatatggtg
     9241 gaaccttcaa aatcatcaat acttattttg gagataaaaa acgatattta caagcaatta
     9301 aagaattgga gcaagagcta tttacagtag cttaatgaaa ggaaagtatg tcaattacat
     9361 catttgtaaa aagaattcaa gatatcactc gaaacgatgc tggtgttaat ggtgatgctc
     9421 aacgtattga gcaaatgtct tggttattat tcttaaaaat ttatgatagc cgtgaaatgg
     9481 tttgggaatt agaagaagac gagtatgagt caattatccc agaggaatta aaatggcgaa
     9541 attgggctca tgctcaaaat ggggaacggg tattgacagg cgatgaatta cttgattttg
     9601 tcaataacaa gttattcaaa gagttgaaag agcttgaaat aacttcaatt atgcctattc
     9661 gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct
     9721 tgttacgcca agtcatcaat gttattgatg aagttgattt caatagccct gaagatcgtc
     9781 attcgtttaa tgatatttac gaaaaaattc ttaaagatat tcaaaatgct gggaactcag
     9841 gagaatttta tacgccacgt gcagcgactg attttattgc cgaagttctt aacccaaaac
     9901 ttggagaatc aatggcagac cttgcttgcg gaacaggagg cttcttgact tcgactctga
     9961 accgtttaag tagtcaacgt aaaactagtg aagataccaa aaaatataat acagctgttt
    10021 ttggtattga aaagaaagca tttcctcatc ttttagcagt tacaaatctg tttcttcacg
    10081 aaattgatga ccctaaaatt gttcatggaa atactttgga gaaaaatgtt cgtgaatata
    10141 cggatgatga aaaatttgac attattatga tgaatccacc ttttggaggg tcagaattag
    10201 aaacaataaa aaataacttt ccagcagaat tacggagttc tgaaacagct gatttattta
    10261 tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg
    10321 gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca aaaactggta gatgagttca
    10381 acttgcatac gattattagg ttgcctcata gtgtctttgc accgtataca ggaatccata
    10441 cgaacattct tttctttgat aaaacaaaga aaacagaaga aacttggttt tatcgtttag
    10501 atatgccaga tggttataaa aatttctcga aaactaagcc gatgaagtca gaacacttca
    10561 atcctgttcg tgactggtgg gaaaatcgtg aagagattct ggaaggtaag ttctacaaat
    10621 ctaaatcatt tacacctagt gaattggctg agttgaatta taatttagac cagtgtggct
    10681 ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt gattcagaat tatcaagcgg
    10741 aaagagcaac tttaaatcat aagattgata atgtattagc tgatattttg cagttgttgg
    10801 aggacaaata atgacaccag aacaacttaa agcaagtatt ctccaaagag cgatggaagg
    10861 gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt gaattattaa agagaattaa
    10921 agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga
    10981 gatatttcgt ggtgatgatg ggaaacatta tgggaagttt gctgatggaa gtactcaaga
    11041 aattgatgtt ccttatgata ttcctgatac ttgggagtgg gtgaggtttt ctacattggt
    11101 tgaaattgtc agaggtggct ctccacgacc aatcaaagat tatcttactt ctgaagtaga
    11161 tggaataaat tggataaaaa taggtgatac tgaaaagggt gaaaagtata taaataatgt
    11221 taaagaaaaa atcaaaaaat caggacttaa caaaactaga tttgtaaaaa aaggtacatt
    11281 tttgttaact aattctatga gttttggtag accttatat
//
LOCUS       DAVGJJ010000064        11268 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000064 DAVGJJ010000000
VERSION     DAVGJJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.064"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            510..1502
                     /locus_tag="VVE35_001508"
     CDS             510..1502
                     /locus_tag="VVE35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3061677.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1504..2322
                     /locus_tag="VVE35_001509"
     CDS             1504..2322
                     /locus_tag="VVE35_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU3061678.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2324..3109
                     /locus_tag="VVE35_001510"
     CDS             2324..3109
                     /locus_tag="VVE35_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061679.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3207..3425
                     /locus_tag="VVE35_001511"
     CDS             3207..3425
                     /locus_tag="VVE35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU3061680.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3410..3607
                     /locus_tag="VVE35_001512"
     CDS             3410..3607
                     /locus_tag="VVE35_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061681.1"
                     /translation="MLATLESEAHKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4078..4164)
                     /locus_tag="VVE35_001513"
                     /pseudo
     CDS             complement(4078..4164)
                     /locus_tag="VVE35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4385..10819
                     /locus_tag="VVE35_001514"
     CDS             4385..10819
                     /locus_tag="VVE35_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU3061682.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKGLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVEDFTSETGKRKQEYDYKYDDKGNIIA
                     YDDDSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAEIKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         3907 a         1701 c         2280 g         3380 t
ORIGIN      
        1 aaaatattca agtgagctaa aaaaatttat tggattttat aatgagatta tttgtgagga
       61 aaatagtttc ctacatgtac gaaagaggtg gtcgagtggg tttaggtagt cggtgcgtga
      121 gttgataatt ctcagggtat ggacttcttt ttcatgaatg aggtaaaaga gcaggtattg
      181 tttagagaca atcattctga gcatattttc tggatagagg gagtatccga ttttatgatc
      241 aaagttaata ccgccctctg gtgagaagat gagtaggttg gtaatttaaa ctattaaaca
      301 gaatttttga ttaaaagtat tattttatga gagaaatcct aatttcacaa tccataggca
      361 atcgcttgca ttctgttttt tattggacta taataggttg gtataaagcc ttctgtagta
      421 ataaaatgta gaaggtgtag aaagtaagga tttagaatat ttgtagttaa aaacacaatg
      481 ttgctattcc ttacgatagg gagatagata tggcaatgat agaagtggaa catcttcaga
      541 aaaattttgt gaagactgtt aaggaaccgg gcttgaaggg ggctttgcgc tcctttattc
      601 atcctgaaaa gcagaccttt gaagcggtca aggatttgac ctttgaggtt ccaaaagggc
      661 agattttagg atttatcggg gcaaatggtg ctgggaagtc gacaaccatt aaaatgctga
      721 caggaatttt gaaaccaaca tctggttttt gtcggattaa cggcaagatt ccccaggata
      781 atcggcaaga ttatgtcaaa gatattggcg tagtctttgg acaacgcacc cagctatggt
      841 gggatttggc tctgcaagag acctacactg tcttaaaaga gatttatgat gtgccagact
      901 cgctctttca taagcgtatg gactttttga atgaagtctt ggatttgaag gactttatca
      961 aggatcccgt gcggactctt tcactgggac aacggatgcg ggcggatatt gcggcctcct
     1021 tgctccacaa tcccaaggtt ctttttttag atgagccgac cattggtttg gacgtttcgg
     1081 ttaaggataa tattcgtcgg gcaattactc agattaatca agaggaagaa actaccattc
     1141 ttttgaccac tcacgatttg agtgatattg agcaactttg tgatcggatt ttcatgattg
     1201 acaaggggca agagattttt gatggaacgg tgagccaact caaggagacc tttggtaaga
     1261 tgaagactct ctcttttgaa ctgctaccag gtcaaagtca tctcgtctct cactatgaag
     1321 gtctgtctga tatgaccatt gatagacaag gaaacagcct caacattgaa tttgatagtt
     1381 ctcgctacca gtcagctgac attatcaagc aaaccctatc tgattttgaa atccgcgatt
     1441 tgaagatggt ggatacggat attgaggata ttatccgtcg cttctaccga aaggagctct
     1501 aggatgatca aattgtggag acgttataaa ccctttatca atgcaggggt tcaggagttg
     1561 attacttacc gagtcaactt tattctctat cggattggcg atgtcatggg ggcttttgtg
     1621 gccttttatc tctggaaggc tgtctttgat tcttcgcaag agtctttgat tcagggcttc
     1681 agtatggcgg atatcaccct ctacatcatc atgagttttg tgaccaatct tctgactaga
     1741 tccgattcgt cctttatgat tggggaggag gtcaaggatg gctccattat catgcgtttg
     1801 ttgcgaccag tgcattttgc ggcctcctat cttttcaccg agcttggttc caagtggttg
     1861 atttttatca gcgttggcct tccattttta agtgtcattg tcttgatgaa aatcatatcg
     1921 ggtcaaggta ttgtagaggt gctaggatta actgtccttt atctttttag cttaacgctc
     1981 gcctatctga ttaacttttt ctttaatatt tgctttggat tttcagcctt tgtgtttaaa
     2041 aatctttggg gttccaacct acttaagact tccatagtgg cttttatgtc ggggagtttg
     2101 attcccttgg cattctttcc aaaggttgtt tcagatattc tctccttttt gcctttttca
     2161 tccttgattt atactccagt tatgatcatt gttggaaaat acgatgccag tcagattctt
     2221 caggcactcc ttttgcagtt cttctggctc ttagtgatgg tgggattgtc tcagttgatt
     2281 tggaaacggg tccagtcctt tatcaccatt caaggaggtt agtatgaaaa aatatcaacg
     2341 aatgcatctg atttttatca gacaatacat caaacaaatc atggaatata aggtggattt
     2401 tgtggttggt gtcttgggag tctttctggc tcaaggcttg aatctcttgt ttctcaatgt
     2461 catctttcaa catattccat tcctagaagg ctggaccttt caagagatag ctttcattta
     2521 tggattttcc ttgattccca agggaatgga ccatctcttt tttgacaatc tctgggcact
     2581 agggcaacgc ctagtccgaa aaggggagtt tgacaagtat ctgactcgtc ccatcaatcc
     2641 tctctttcac atcctagttg aaacctttca gattgatgcc ttgggtgaac tcttagtcgg
     2701 tggtatttta ttgggaacaa cagtgaccag cattgtttgg actcttccaa aattcctgct
     2761 tttcctagtt tgtattcctt ttgcgacctt gatttatact tctcttaaaa tcgcaacagc
     2821 cagtatcgcc ttttggacta agcagtcagg cgccatgatt tacatcttct atatgttcaa
     2881 tgactttgct aagtatccga tttctattta caattctctt cttcgttggt tgattagctt
     2941 tatcgtgcct ttcgccttta cagcctacta tccagctagc tatttcttac aggaaaagga
     3001 tgtgttcttt aacgtaggag gtttgatgtt gatttctctg gttttctttg ttatttccct
     3061 taaactttgg gataagggct tagattccta cgaaagtgcg ggttcgtaaa agctaaagta
     3121 agactaaaat caagaaagga acttatgatg tttgtaattg aagaagtcaa ggatgaaaat
     3181 caaaaaaggc agttgtcgct gaggttttga aggatttgcc agaatggttt ggaatcccag
     3241 aaagcacaca agcctatata gaaggaacca cgacactgca agtttggacc gcctatcagg
     3301 agagtgattt gactggattt gtaagcttat cctattcgag tgaagattgt gcagagattg
     3361 attgtctcgg cgtaaaaaag cttatcaagg tagaaaaatt gggagccaat tgcttgctac
     3421 tttagagagt gaagctcata aaaaagttgg ttatctgcag gtcaaaacag tggcagaagg
     3481 ttctaataaa gattatgatc gaacaaatga cttttatcga ggtcttggct ttaaaaagtt
     3541 agagattttt cctcaactat ggaatccgca aaatccttgt cagattttga ttaaaaagct
     3601 tgaataatat tacttgacat ctattctcag agtgctatac tgtaagtgta atcgccgatt
     3661 tagcttagtt ggtagagcaa ggcactcgta aagcctaggt tataggtaga taaacgactg
     3721 aggatttgaa aaaatagata ggtagaagat aaccgttaag ccttactctt agcggttatt
     3781 tatattgttt aatagcgcta atatttcatc aattatgcct gttttcgtgt ttctggtagt
     3841 tgttcaagtt tattgctact atttttgatg gtatgaatgt gcttataatg tatcccggtt
     3901 aacgaaagtt ttggacttat actcttcgaa aatctcttca aaccacgtca acgtcgcctt
     3961 gccgtgcgta tggttactga ctacgtcagt tctatccaca acctcaaaac agtgttttga
     4021 gctgactacg tcagttctat ccacaacctc aaaacagtgt tttgagctga ctacgtcagt
     4081 tctatccaca acctcaaaac agtgttttga gcaacctgcg gctagtttcc tagtttgctc
     4141 tttgattttc attgagtact aatcttattt tttaacgtgt gctaaaaaat aaattaatgc
     4201 atgttaattt ttcttgaatt aaattttttt aaatgatata ctgtttttat ggagagataa
     4261 caattatata taaatgtact actccatttc ctagataata cttagtaaaa ctttttataa
     4321 caaaggctag caaagttaaa gtttattatc tattggaagt taaataaata tgtaaggaat
     4381 taaaatgaaa aaaagtacgg tattgtcatt aaccacagct gcagttattt tagcagccta
     4441 tgcccctaat gaggtagtct tagcagacac atctaactct gaagatgctt taagcatctc
     4501 tgataaagaa aaagtagtag tagataagga aacagaaaat aaagagaaac ataaagatat
     4561 tcataatgct atagaaactt caaaggatac tgaagaaaag aaaacaacag ttattgagga
     4621 aaaagaagtt gttagtaaaa atcctgtgat agacactaaa actagcaatg aagaagcaag
     4681 aatcaaagaa gattccaatc aatcccaagg agataatgca cactcgtctg caaataaagg
     4741 cacagaaaat ccgaaaaaag aagatagact tgtctatatt gctgaattta aagataaaga
     4801 atctggagaa aaagcaatca agggactatc aaatcttaag aatacaaaag ttttatatac
     4861 ttatgataga atttttaacg gtagtgccat agaaacaact ccagataact tggacaaaat
     4921 taaacaaata gaaggtattt catcggttga aagggcacaa aaagtccaac ccatgatgaa
     4981 tcatgccaga aaggaaattg gagttgagga agctattgat tacctaaagt ctatcaatgc
     5041 tccgtttggg aaaaattttg atggtagagg tatggtcatt tcaaatatcg atactggaac
     5101 agattataga cataaggcta tgagaatcga tgatgatgcc aaagcctcaa tgagatttaa
     5161 aaaagaagac ttaaaaggta ctgataaaaa ttattggttg agtgataaaa tccctcatgc
     5221 gttcaattat tataatggtg gcaaaatcac tgtagaaaaa tatgatgatg gaagggatta
     5281 ttttgaccca catgggatgc atattgcagg gattcttgct ggaaatgata ctgaacaaga
     5341 tatcaaaaac tttaacggca tagatggaat tgcacctaat gcacaaattt tctcttacaa
     5401 aatgtattct gacgcaggat ctgggtttgc gggtgatgaa acaatgtttc atgctattga
     5461 agattctatc aaacacaacg ttgatgttgt ttcggtatca tctggtttta caggaacagg
     5521 tcttgtaggt gagaaatatt ggcaagctat tagggcatta agaaaagcag gcattccaat
     5581 ggttgtcgct acgggtaact atgcgacttc tgcttcaagt tcttcatggg atttagtagc
     5641 caataatcat ctgaaaatga ccgacactgg aaatgtaaca cgaactgcag cacatgaaga
     5701 tgcgatagcg gtcgcttctg ctaaaaatca aacagttgag tttgataaag ttaacatagg
     5761 tggagaaagt tttaaataca gaaatatagg ggcctttttc gataagaata aaatcacaac
     5821 aaatgaagat ggaacaaaag ctcctagtaa attaaaattt gtatatatag gcaaggggca
     5881 agaccaagat ttgataggtt tggatcttag gggcaaaatt gcagtaatgg atagaattta
     5941 tacaaaggat ttaaaaaatg cttttaaaaa agctatggat aagggtgcac gcgccattat
     6001 ggttgtaaat actgtaaatt actacaatag agataattgg acagagcttc cagctatggg
     6061 atatgaagcg gatgaaggta ctaaaagtca agtgttttca atttcaggag atgatggtgt
     6121 aaagctatgg aacatgatta atcctaataa aaaaactgaa gtcaaaagaa ataataaaga
     6181 agattttaaa gataaattgg agcaatacta tccaattgat atggaaagtt ttaattccaa
     6241 caaaccgaat gtaggtgacg aaaaagagat tgactttaag tttgcacctg acacagacaa
     6301 agaactctat aaagaagata tcatcgttcc agcaggatct acatcttggg ggccaagaat
     6361 agatttactt ttaaaacccg atgtttcagc acctggtaaa aatattaaat ccacgcttaa
     6421 tgttattaat ggcaaatcaa cttatggcta tatgtcagga actagtatgg cgactccaat
     6481 cgtggcagct tctactgttt tgattagacc gaaattaaag gaaatgcttg aaagacctgt
     6541 attgaaaaat cttaagggag atgacaaaat agatcttaca agtcttacaa aaattgccct
     6601 acaaaatact gcgcgaccta tgatggatgc aacttcttgg aaagaaaaaa gtcaatactt
     6661 tgcatcacct agacaacagg gagcaggcct aattaatgtg gccaatgctt tgagaaatga
     6721 agttgtagca actttcaaaa acactgattc taaaggtttg gtaaactcat atggttccat
     6781 ttctcttaaa gaaataaaag gtgataaaaa atactttaca atcaagcttc acaatacatc
     6841 aaacagacct ttgactttta aagtttcagc atcagcgata actacagatt ctctaactga
     6901 cagattaaaa cttgatgaaa catataaaga tgaaaaatct ccagatggta agcaaattgt
     6961 tccagaaatt cacccagaaa aagtcaaagg agcaaatatc acatttgagc atgatacttt
     7021 cactataggc gcaaattcta gctttgattt gaatgcggtt ataaatgttg gagaggctaa
     7081 aaacaaaaat aaatttgtag aatcatttat tcattttgag tcagtggaag aaatggaagc
     7141 tctaaactcc aacgggaaga aaataaactt ccaaccttct ttgtcgatgc ctctaatggg
     7201 atttgctggg aattggaacc acgaaccaat ccttgataaa tgggcttggg aagaagggtc
     7261 aagatcaaaa acactgggag gttatgatga tgatggtaaa ccgaaaattc caggaacctt
     7321 aaataaggga attggtggag aacatggtat agataaattt aatccagcag gagttataca
     7381 aaatagaaaa gataaaaata caacatccct ggatcaaaat ccagaattat ttgctttcaa
     7441 taacgagggg atcaacgctc catcatcaag tggttctaag attgctaaca tttatccttt
     7501 agattcaaat ggaaatcctc aagatgctca acttgaaaga ggattaacac cttctccact
     7561 tgtattaaga agtgcagaag aaggattgat ttcaatagta aatacaaata aagagggaga
     7621 aaatcaaaga gacttaaaag tcatttcgag agaacacttt attagaggaa ttttaaattc
     7681 taaaagcaat gatgcaaagg gaatcaaatc atctaaacta aaagtttggg gtgacttgaa
     7741 gtgggatgga ctcatctata atcctagagg tagagaagaa aatgcaccag aaagtaagga
     7801 taatcaagat cctgctacta agataagagg tcaatttgaa ccgattgcgg aaggtcaata
     7861 tttctataaa tttaaatata gattaactaa agattaccca tggcaggttt cctatattcc
     7921 tgtaaaaatt gataacaccg cccctaagat tgtttcggtt gatttttcaa atcctgaaaa
     7981 aattaagttg attacaaagg atacttatca taaggtaaaa gatcagtata agaatgaaac
     8041 gctatttgcg agagatcaaa aagaacatcc tgaaaaattt gacgagattg cgaacgaagt
     8101 ttggtatgct ggcgccgctc ttgttaatga agatggagag gttgaaaaaa atcttgaagt
     8161 aacttacgca ggtgagggtc aaggaagaaa tagaaaactt gacaaagacg gaaataccat
     8221 ttatgaaatt aaaggtgcgg gagatttaag gggaaaaatc attgaagtca ttgcattaga
     8281 tggttctagc aatttcacaa agattcatag aattaaattt gctaatcagg ctgatgaaaa
     8341 ggggatgatt tcctattatc tagtagatcc tgatcaagat tcatctaaat atcaaaagct
     8401 tggcgagatt gcagaatcta aatttaaaaa tttaggaaat ggaaaagagg gtagtctaaa
     8461 aaaagataca actggggtag aacatcatca tcaagaaaat gaagagtcta ttaaagaaaa
     8521 atctagtttt actattgata gaaatatttc aacaattaga gactttgaaa ataaagactt
     8581 aaagaaactc attaaaaaga aatttagaga agttgaggac tttacaagtg aaactggtaa
     8641 gaggaaacaa gaatacgatt ataaatacga tgataaggga aatatcattg cttatgacga
     8701 tgatagtgcc ttacaatatg aaactgaaaa gcttgatgaa ataaaatcaa agatttatgg
     8761 tgttctaagc ccgtctaaag atggacactt tgaaattctt ggaaagataa gcaatgtttc
     8821 taaaaatgcc aaggtatatt atggaaataa ctataaattt atagaaatca aagcgactaa
     8881 gtatgactca cattcaaaaa cgatgacatt tgatttatac gctaatatta atgatattgt
     8941 ggatggatta gcttttgctg gagatatgcg gttttttgtt aaagatgatg atcgaataaa
     9001 agctgaaatt aaaattagaa tgcctgaaaa aaataaggaa actaaagcag aatatcccta
     9061 tgtatcaagt tatgggaatg taatcgaatt aggagaagga gatctttcaa agaacaaacc
     9121 agacaattta actaaaatgg aatctggtaa aatctattct gattcagaaa aacaacaata
     9181 tctgttaaag gataacatca ttctaagaaa aggctatgca ctaaaagtga ctacctataa
     9241 tcctggaaaa acggatatgt tagaagggaa tggagtctat agcaaggaag atatagcaaa
     9301 aatacaaaag gccaatccta atctaagagt cctttcagaa acaacaattt atgctgatag
     9361 tagaaatgtt gaagatggaa gaagtaccca agctgtatta atgtcggctt tggacggctt
     9421 taacattata aggtatcaag tgtttacatt taaaatgaac gataaagggg aagctatcga
     9481 taaagacgga aatcttgtga cagattcttc taaacttgta ttatttggta aggatgataa
     9541 agaatacact ggagaggata agtccaatgt agaagctata aaagaagatg gctccatgtt
     9601 atttattgat accaaaccag taaacctttc aatggataag aactacttta atccatctaa
     9661 atctaataaa atttatgtac gaaatccaga attttattta agaggtaaga tttctgataa
     9721 gggtggtttt aactgggaat tgagagttaa tgaatcggtt gtagataatt atttaatcta
     9781 cggagattta cacattgata acactagaga ttttaatatt aagctgaatg ttaaagacgg
     9841 tgacatcatg gactggggaa tgaaagacta taaagcaaac ggatttccag ataaggtaac
     9901 agatatggat ggaaatgttt atcttcaaac tggctatagc gatttgaatg ctaaagcagt
     9961 tggagtccac tatcagtttt tatatgataa tgttaaacct gaagtaaaca ttgatcctaa
    10021 gggaaatact agtatcgaat atgctgatgg aaaatctgta gtctttaaca tcaatgataa
    10081 aagaaataat ggattcgatg gtgagattca agaacaacat atttatgtaa atggaaaaga
    10141 atatacatca tttgatgata ttaaacaaat aatagacaag acactaaaca ttaagattgt
    10201 tgtaaaagat tttgcaagaa atacaaccgt aaaagaattc attttaaata aagatacggg
    10261 agaggtaagt gaattaaaac ctcatagggt aactgtgacc attcaaaatg gaaaagaaat
    10321 gagttcaacg atagtgtcgg aagaagattt tattttacct gtttataagg gtgaattaga
    10381 aaaaggatac caatttgatg gttgggaaat ttctggtttc gaaggtaaaa aagacgctgg
    10441 ctatgttatt aatctatcaa aagatacctt tataaaacct gtattcaaga aaatagagga
    10501 gaaaaaggag gaagaaaata aacctacttt tgatgtatcg aaaaagaaag ataacccaca
    10561 agtaaaccat agtcaattaa atgaaagtca cagaaaagag gatttacaaa gagaagatca
    10621 ttcacaaaaa tctgattcaa ctaaggatgt tacagctaca gttcttgata aaaacaatat
    10681 cagtagtaaa tcaactacta acaatcctaa taagttgcca aaaactggaa cagcaagcgg
    10741 agcccagaca ctattagctg ccggaataat gtttatagta ggaatttttc ttggattgaa
    10801 gaaaaaaaat caagattaag acaaaagcta tagaaaaaaa tggtttatgt actgagatta
    10861 gatagtgagg tgatgacata gttttgtgaa aacagccatt tataactcaa ttatttagtt
    10921 tacttcacta ttgatactat ttggagttat taatggactt agtttagata actaatgaat
    10981 tgattgaaag ggttagtatt gacaatattg gtcatattga ttagaaaata gagtctatca
    11041 aaatttaaag gctaatagag gtgatgagac aatttcaagg aattaagtag cgtttggttg
    11101 cttaattcct ttttatttgg aaggaagttt ttcgtaaccg cccaataacg aagtctattg
    11161 gacggttacg atatgatcac tgacttcgtc agtcttatct acaacctcaa aactgtgttt
    11221 tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattga
//
LOCUS       DAVGJJ010000065        11261 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000065 DAVGJJ010000000
VERSION     DAVGJJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.065"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            266..2716
                     /locus_tag="VVE35_001515"
     CDS             266..2716
                     /locus_tag="VVE35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU3061683.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVENGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            2753..3976
                     /locus_tag="VVE35_001516"
     CDS             2753..3976
                     /locus_tag="VVE35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU3061684.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            3987..4541
                     /locus_tag="VVE35_001517"
     CDS             3987..4541
                     /locus_tag="VVE35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU3061685.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            4626..5189
                     /locus_tag="VVE35_001518"
     CDS             4626..5189
                     /locus_tag="VVE35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU3061686.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            5204..7150
                     /gene="gyrB"
                     /locus_tag="VVE35_001519"
     CDS             5204..7150
                     /gene="gyrB"
                     /locus_tag="VVE35_001519"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU3061687.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            7232..8959
                     /gene="ezrA"
                     /locus_tag="VVE35_001520"
     CDS             7232..8959
                     /gene="ezrA"
                     /locus_tag="VVE35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU3061688.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(9688..10011)
                     /gene="tnpB"
                     /locus_tag="VVE35_001521"
                     /pseudo
     CDS             complement(9688..10011)
                     /gene="tnpB"
                     /locus_tag="VVE35_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(10272..10571)
                     /locus_tag="VVE35_001522"
     CDS             complement(10272..10571)
                     /locus_tag="VVE35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU3061689.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(10736..11191)
                     /locus_tag="VVE35_001523"
     CDS             complement(10736..11191)
                     /locus_tag="VVE35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3061690.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYSNPAYLY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
BASE COUNT         3414 a         2029 c         2349 g         3469 t
ORIGIN      
        1 gtcgcttttc attatagatc ttatgggact ttttttctac aataaaatag gctccataat
       61 atctatagtg gatttaccca ctacaaatat tatagagcca catttcttct ctcaattttt
      121 cgtatttctt cactatttta caaaaatcag caggctagaa tgctctattc gatgggattt
      181 ttaagaaaag tagtgttctt gagtttgaaa attatcctat gtttgcaggt gccaaatggc
      241 cctttttttg gtataatttt ttataatgaa aacgattggt aatcgctatg ttgtggtgga
      301 tttagaggca actagcacag gtagtaaggc taaaattatc caagtgggaa ttgtcgtgat
      361 tgaggacgga gaaatcgtcg atcactatac gacggatgtc aatccacatg aacccttgga
      421 tgctcatatc aaagaactga caggattgac agaccaacgt ctggcgcaag cacctgattt
      481 ttcgcaagtt gccagaaaaa tatttgactt ggtggagaat gggatttttg tagcccataa
      541 tgttcagttt gatgctaatc tcttggcgga aaatttattt tttgaaggct atgagctaag
      601 aaaccctcgt gttgatacgg tcgaattggc ccaggtcttt ttccctgaac tggaaaaata
      661 tagcttgccg attttgtgtc gagaattagg aattcctctt aaacacgcac acacagccct
      721 ttcagatgcc caagctacag cagaattact tcttttttta cgggaaaaga tgacccagct
      781 tcctaaaggt ctcttggaac gcttgctgga aatggctgac gctctcctat atgagtccta
      841 cctggttatt gaggaaactt atcgcaacca atctatcctg agttctccag acttggtcca
      901 agttcaaggt ctgtatttta agaaaacggc agcttctctg gagctacgaa aactatctca
      961 agacttttct aaaaatattt ctctgttgaa ccttgaagtg agggaggaac aagaaagttt
     1021 tgctaaagag gttggcttgc tattgaaaga tgaacctgtc tctctgattc aagcgccgac
     1081 agggattggg aaaacctatg gctatctctt acccgcttta tctcaatcca aagagcgaca
     1141 aattgttctt agtgttccga caaagattct tcaaaatcaa atcatggaag aagaaggtaa
     1201 acgtctcaag gaagtgttcc atacagatat tcatagctta aagggaccac aaaattatct
     1261 gaagttggat gccttttatc attccttgca ggaaaatgat gaaaatcgct tatttagacg
     1321 ctttaaaatg caagtcttgg tctggcttac tgagacagag acaggagatt tggatgaaat
     1381 cgggcaactc taccgttacc aacattttct agcagacctt cgtcatgatg ggaatttatc
     1441 atcccagagc ttatttgtga cggaagattt ttggaaacgt agtcaagaaa gggcagagac
     1501 ttgcaagctt ttagtgacta atcatgccta tctcgtaacc agacttgaag ataatcctga
     1561 atttgtcagt gaccgtttac tgattattga tgaagtacaa aagattttgt tagctctaga
     1621 aaatctgctt caagagacct acgatataca atctattatc gatttaattg ataaggcttt
     1681 agtaggagaa gaaaacaggg ttcaacaacg gatactagaa agtattcgct ttgagtgtct
     1741 ctacttgata gaacaatttc agtctggtaa atctaggaaa aatatcttag attctctgga
     1801 caatctccat cagtattttt cagaattaga agtggaaggc tttgatgagc tggttcgcta
     1861 ttttacagct gaaggtgatt actggcttga agtaactgaa acgagtcaaa agaaaattca
     1921 gatttcttct acaaaatcag gccgtactct tctgtcctct ttacttcctg agagttgcca
     1981 agtcttggga gtatcggcta ctcttgagat tagtcagagg gtttctttgg cagacctttt
     2041 aggctatcct gaagccaaat ttgtcaagat tgaatctcgg ggaaaacagg aacaagaagt
     2101 ggttatggtc aaagatttcc ctctggtaac agaaacctcc ttagaagtct atgccagaga
     2161 ggtagctgct ttactagtgg aaattcaagc tttccagcaa ccgattttgg ttctctttac
     2221 cgctaaagac atgcttctag cagtatcgga tttacttaca gttagccact tggcccagta
     2281 taaaaatggg gatgttcatc agctaaagaa acgctttgaa aaaggtgaac aacaaatctt
     2341 gcttggtgca gcaagtttct gggagggagt tgatttttca agccatcctt ctgtgattca
     2401 agttgtaccg aggcttcctt tccaaaatcc tcaagaaccc ttgacgaaaa agattaatca
     2461 agaactgaat caagaaggga aaaatgcctt ttatgattat caattgccaa tggccattat
     2521 tcgtttaaaa caggctttgg gaagaagtat gagacgtgaa taccaacgtt ccttaactct
     2581 tattttggat aggagaatcg tcggaaaacg atacggcaaa caaatagtag catctctagc
     2641 agaagaagcg actgttaaaa ccatctctcg atccgaagtt gacgaggcta ttgatagatt
     2701 ttttaatgaa ctttgataaa tagtattgta tgaaagtata aggttagtat atatgaaacg
     2761 ttctctcgac tcaagagtcg attacagttt gctcttgcca gtattttttc tactggtcat
     2821 cggtgtggtg gctatctata tagccgttag tcatgattat cccaataata ttctgcccat
     2881 tttagggcag caggtcgcct ggattgcctt ggggcttgtg attggttttg tggtcatgct
     2941 ctttaataca gaatttcttt ggaaggtgac cccctttcta tatattttag gcttgggact
     3001 tatgatcttg ccgattgtat tttataatcc aagcttagtt gcatcaacgg gtgccaaaaa
     3061 ctgggtatca ataaatggaa ttaccctatt tcaaccgtca gaatttatga agatatccta
     3121 tatcctcatg ttggctcgtg tcattgtcca atttacaaag aaacataagg aatggagacg
     3181 cacggtttcg ctggactttt tgttaatttt ctggatgatt ctctttacca ttccagtcct
     3241 agttctttta gcacttcaaa gtgacttggg gacggctttg gtttttgtag ccattttctc
     3301 aggaatcgtt ttattatcag gggtttcttg gaaaattatt atcccagtat ttgtgactgc
     3361 tgtaacagga gttgctggtt tcttagctat ctttattagc aaggacggac gagcttttct
     3421 tcaccagatt ggaatgccga cctaccaaat caatcggatt ttggcttggc tcaatccctt
     3481 tgagtttgcc caaacaacga cttaccagca ggctcaaggg cagattgcca ttgggagtgg
     3541 tggcttattt ggtcagggat ttaatgcttc gaatctgctt atcccagttc gagagtcaga
     3601 tatgattttt acggttattg cagaagattt tggctttatt ggctctgtcc tggttattgc
     3661 cctctatctc atgttgattt accgtatgtt gaagattact cttaaatcaa ataaccagtt
     3721 ctacacttat atttccacag gtttgattat gatgttgctc ttccacatct ttgagaatat
     3781 cggtgctgtg actggactac ttcctttgac ggggattccc ttgcctttca tttcgcaagg
     3841 gggatcagct attatcagta atctgattgg tgttggtttg cttttatcga tgagttacca
     3901 gactaatcta gctgaagaaa agagcggaaa agtcccattc aaacggaaaa aggttgtatt
     3961 aaaacaaatt aaataaggag aaaatcatgg taaaagtagc agttatgtta gctcagggct
     4021 ttgaagaaat tgaagccttg acagttgtag atgtcttgcg tcgagccaat atcacatgtg
     4081 atatggttgg ttttgaagag caagtaacgg gttcgcatgc aatccaagta agagcagatc
     4141 atgtctttga tggaaattta tcagactatg atatgattgt tcttcctgga ggtatgcctg
     4201 gttctgcaca tttacgtgat aatcagacct tgattcaaga attgcaaagc ttcgagcaag
     4261 aagggaagaa actagcagcc atttgtgcgg caccaattgc cctcaatcaa gcagagatat
     4321 tgaaaaataa gcgatacact tgttatgacg gcgttcaaga gcaaatcctt gatggtcact
     4381 acgtcaagga aacagtagtg gtagatggtc agttgacaac cagtcggggt ccttcaacag
     4441 cccttgcctt tgcctacgag ttggtggagc aactaggagg ggacgcagag agtttacgaa
     4501 caggaatgct ctatcgagat gtctttggta aaaatcagta aaacgggagt tattctctcg
     4561 ttttttatgt ggaaaactca gggaaatcat cgcttttttc ataaaaaaat gctataatga
     4621 agggtatgaa atatcacgat tacatctggg atttaggtgg aactttactg gataattatg
     4681 aaacttcaac agctgcattt gttgaaacat tggcactgta tggtatcaca caagaccatg
     4741 acagtgtcta tcaagcttta aaggtttcta ctccttttgc gattgagaca ttcgctccca
     4801 atttagagaa ttttttagaa aagtacaagg aaaatgaagc cagagagctt gaacacccga
     4861 ttttatttga aggagtttct gacctattgg aagtcatttc aaatcaaggt ggccgtcatt
     4921 ttttggtctc tcatcgaaat gatcaggttt tggaaatttt agaaaaaacc tctatagcag
     4981 cttattttac agaagtggtg acttctagct caggctttaa gagaaagcca aatcccgaat
     5041 ccatgcttta tttaagagaa aagtatcaga ttagctctgg tcttgtcatt ggtgatcggc
     5101 cgattgatat cgaagcaggt caagctgcag gacttgatac ccacttgttt accagtatcg
     5161 tgaatttaag acaagtatta gacatataag aaaaaggaat aagatgacag aagaaatcaa
     5221 aaatctgcag gcacaggatt atgatgccag tcaaattcaa gttttagagg gcttagaggc
     5281 tgttcgtatg cgtccaggga tgtacattgg atcaacctca aaagaaggtc ttcaccatct
     5341 agtctgggaa attgttgata actcaattga cgaggccttg gcaggatttg ccagccatat
     5401 tcaagttttt attgagccag atgattcgat tactgttgtg gatgatgggc gtggtatccc
     5461 agtcgatatt caggaaaaaa caggccgtcc tgctgttgag accgtcttta cagtccttca
     5521 cgctggagga aagttcggcg gtggtggata caaggtttca ggtggtcttc acggggtggg
     5581 gtcgtcagta gttaatgccc tttccactca attagacgtt catgtccata aaaacggtaa
     5641 gattcattac caagaatacc gtcgtggtca tgttgttgca gaccttgagg tggttgggga
     5701 tacagataga actggaacaa cggttcactt cataccggac ccaaaaatct ttactgaaac
     5761 aacaaccttt gattttgata aattaaataa acggattcaa gagttggcct ttctaaatcg
     5821 cggtcttcaa atttctatca ctgataagcg ccaaggtttg gaacaaacca agcattatca
     5881 ttatgaaggt gggattgcta gttacgttga atatatcaac gagaacaagg atgtaatctt
     5941 tgatacacca atctatacag acggtgagat ggatgatatc acagttgagg tagccatgca
     6001 gtacacaaca ggttaccatg aaaatgtcat gagtttcgcc aataatattc atacccatga
     6061 aggtggaacg catgaacaag gtttccgtac ggcactgact cgtgttatca atgattatgc
     6121 tcgtaagaat aagttactga aagacaatga agataattta acaggggaag atgttcgcga
     6181 aggcttaact gcagttatct cagttaaaca cccaaatcca cagtttgaag gacaaaccaa
     6241 gaccaaattg ggaaatagcg aagtggtcaa gattaccaat cgcctcttca gtgaagcctt
     6301 ctccgatttc ctcatggaaa atccacagat tgccaaacgt atcgtggaaa aagggatttt
     6361 agctgccaag gctcgtgtgg ctgccaagcg tgcgcgtgaa gtcacacgta aaaaatctgg
     6421 tttggaaatt tccaaccttc cagggaaact agcagactgt tcttctaata accctgctga
     6481 aacagaactc ttcatcgtcg aaggagactc agctggtgga tcagccaaat ctggtcgtaa
     6541 ccgtgagttt caggctatcc ttccaattcg cggtaagatt ttgaacgttg aaaaagcaag
     6601 tatggataag attctagcta acgaagaaat tcgtagtctt ttcacagcca tgggaacagg
     6661 atttggcgca gaatttgatg tttcgaaagc ccgttaccaa aaactcgttt tgatgaccga
     6721 tgccgatgtc gatggagccc atattcgtac ccttctttta accttgattt atcgttatat
     6781 gaaaccaatc ctagaagctg gttatgttta tattgcccaa ccaccaatct atggtgtcaa
     6841 ggttggaagc gagattaaag aatatatcca gccgggtgca gatcaagaaa tcaaactcca
     6901 agaagcttta gcccgttata gtgaaggtcg taccaaaccg actattcagc gttataaggg
     6961 gctaggtgaa atggacgatc atcagctgtg ggaaacaacc atggatcccg aacatcgctt
     7021 gatggctaga gtttctgtag atgatgctgc agaagcagat aaaatctttg atatgttgat
     7081 gggggatcga gtagagcctc gtcgtgagtt tatcgaagaa aatgctgtct atagtacact
     7141 tgatgtctaa aaaataggga gaagtagttc ccttggtcta aaaaatatga tataatcgtt
     7201 acgattgttt caagtaaaaa aggagtttga tatgtctaat ggacaactaa tttatttaat
     7261 ggttgcaatt gcagtcattt tagttctggc ttatgtagtg gcaatctttc tacgtaagcg
     7321 aaacgagggg agattagagg cgctagaaga aagaaaagaa gaactataca atcttccagt
     7381 aaatgatgaa gtagaagctg taaaaaatat gcacttgatt ggacaaagtc aagtggcttt
     7441 ccgtgaatgg aatcaaaaat gggtcgattt atctctcaac tcttttgccg atattgaaaa
     7501 taatctcttt gaagcagaag gctataacca ttcatttcgt tttctcaagg ccagtcatca
     7561 aattgaccaa attgagagtc aaattacttt gattgaagaa gatattgcgg caattcgcaa
     7621 tgctttggca gacttagaga agcaagaatc taaaaatagt ggtcgtgttc ttcatgcttt
     7681 ggatttattt gaggaacttc agcatagagt tgctgaaaat tcagaacagt atggtcaagc
     7741 cttggatgaa attgaaaaac aattagaaaa tatccaatct gaattttcac aatttgtaac
     7801 cttgaattca tcgggtgacc ctgtggaagc cgcagtgatt ttggataata cagaaaatca
     7861 cattttggcc ttaagtcata ttgtggatcg tgttccagcc ttggttacga cgctttctac
     7921 agaattgcca gatcaattac aggatttgga agccggttat cgtaaactaa ttgatgctaa
     7981 ttatcatttt gttgaaacgg atattgaagc gcgtttccac ttgctttatg aagcattcaa
     8041 gaaaaaccaa gagaatattc gtcagttgga attggataat gccgaatatg agaatggaca
     8101 ggcacaagag gaaatcaatg ccttgtatga tatttttact cgagaaattg ctgctcagaa
     8161 agtagtggaa aatctacttg caactcttcc aacttacctt caacatatga aagagaataa
     8221 tactttattg ggagaagata ttgcacgttt gaacaagacc tatttacttc ctgagacagc
     8281 tgcaagccat gttcgtcgta ttcagacaga attagagagt tttgaggcag ctattgttga
     8341 ggtaacttca aatcaagaag aaccaaccca agcttattca gttcttgaag aaaatcttga
     8401 ggatttacaa actcaactaa aagatattga agatgagcaa atttcagtta gtgagcgcct
     8461 gacacaaatt gagaaagatg atattaatgc acgtcaaaag gccaatgttt atgtcaatcg
     8521 tctccatact atcaagcgat acatggaaaa acgcaatctg ccaggtattc cacaaacttt
     8581 cttgaagtta ttctttacgg caagcaataa taccgaggat ttaatggttg agttagaaca
     8641 aaaaatgatt aacattgaat ctgttacccg agttcttgaa attgcaacga atgatatgga
     8701 agctttagaa acggaaactt ataatattgt acaatatgca actttgacag agcaactctt
     8761 gcaatattct aaccgctatc gctcatttga tgaacgcatt caagaagcat ttaacgaagc
     8821 tttagatatt tttgaaaaag aatttgatta tcacgcttca tttgacaaga tttctcaagc
     8881 attggaagtg gcagagcctg gtgtaactaa tcgctttgtt acctcatatg agaaaacacg
     8941 tgaaacgatt cgtttttaat aaaagaaaaa gattttattg tgtgaggagc agaatcaaat
     9001 ctttttctat agttgtgggg agatttactt cattttctcc tgagattgag tttttaccca
     9061 gccgatttat ccaccacctc aaaacagtgt tttatactct tcgaaaatct cttcaaatca
     9121 cgtcagcgtc gccttaccgt actcaagtac agcctgaggc tagcttctta gtttgctttt
     9181 tgattttcat ttagtattaa agtgatttcg tcagtcttat ctgcagcttc aaatctgtac
     9241 tttgagtaac ttggtaaccg tccaataacg aagtctattg aaaaatctcc agactagaga
     9301 actcacggat agttcctaat ctggagattt cttatttgca cttttcttgt acaactttag
     9361 tctacggtaa ataggcctct aaaacctctt tgtttacgag agtttcctcg tttggaagac
     9421 attctagaag ataggataga tatttctcgc tatttatacc agactaaaat aaaaaagcac
     9481 tatataatag taatatgaaa tcaactaaag aagaaatcca aaccatcaaa acacttttaa
     9541 aagactctcg tacagctaaa tatcataaac accttcaaat cgttctatag taaaatgaaa
     9601 taagaacagt acaaatcgat taggacagtc aaattgattt ctaacaatgt tttagaagta
     9661 ggggtgtact attctagttt aaatccacta tatttgggga gtgatagaaa agcccttcat
     9721 aagccagtct acttgttctg gtgtgagagc tttgacatcc ttttctgtac ttggtcaagt
     9781 cagttttccg ttctcaaagc gtttatatag tagccaaaat ccttgaccat cccagtaaag
     9841 ggctttaaag cggtctttac gtccaccaca aaagagaaag acttgaccgg agaaaggatc
     9901 caattcaaag tgggttttaa ctacataggc taatgagtct attccctgcc tcatatctgt
     9961 cttgccacaa acaatgtgaa cttgacctaa atcacttagt tgaatcatca tagtacaata
    10021 cctttcctcc gataattatt ttttatctag tatactggaa gttggggaat taggatagat
    10081 accttgttat gacgcgctta cgtaacttgt aactagctgc ctagtttgat ctttgcttct
    10141 tcattgatta gcagtagatt tcaaaatgat aaaaacgcat aatagtgggt gtagaaactc
    10201 ttgctactat gcgttttatt atggaaagac ttattggact ttctctcaaa tcgagttttt
    10261 actcaatttt cttacttgat tgggattgaa attccaatta atttctctga gtagagtgtc
    10321 ttgatattgg cttcatcaac agaggtctta tcaattttac gtttcaagaa aaattcttga
    10381 atggtttcga tttcaggctc acgaatagca cggtgtttgt ttgagatgag gatttcatag
    10441 tgaagcggag cttgggtaaa aataacatct gtattccctg cagaataaac ctcaacaagg
    10501 gttgcatcgg tactttctag ctgacttttt accagttgcg agtgtgagtt tgtcgtattg
    10561 ataagcttca taatatttcc tccgattttc taattctatt atagcacttt ttgaataaag
    10621 tcgcttgatt tatactcaat gaaaataaaa gagcaaacta ggaagctagc cgcaggctat
    10681 acttgagtac ggtaaggcga cgctgacgtg gtttgaattt tattttcatt gagtattagt
    10741 caatcttatg ctgttttttc caagattcaa tggcccattt atggctacca cgtttaaggt
    10801 ttttgatagc ctcgtcaata gggaaccagg caatatgatt aaagttttct agtggctttt
    10861 gtacttcttt gaaaggagtt gcttcataga ggtaggcagg attggagtag taggtatcac
    10921 gatgacgaga atagaaatat tcgtcagctt gtccgtaata ggtaccaatt tctgctgtga
    10981 aaccaagctc ttcaatcaac tcacgcttta gggcttcctg atgattttca cctgcttcaa
    11041 tttctccaca tggtaggaac caagcaccat ttggttcttg aacaagaaca atttgttttt
    11101 gttcaggatt agggataact gcatatacgc catagcgagc aatatagtct gtattcgctt
    11161 ttttctccga aagttgggtt tgccattgca ttttcctcat tatctagtat cgttattatt
    11221 atagtgaaat gaaccaaaaa tagtacacaa tgtggtataa t
//
LOCUS       DAVGJJ010000066        11216 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000066 DAVGJJ010000000
VERSION     DAVGJJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.066"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            128..508
                     /locus_tag="VVE35_001524"
     CDS             128..508
                     /locus_tag="VVE35_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061691.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            1043..1735
                     /locus_tag="VVE35_001525"
     CDS             1043..1735
                     /locus_tag="VVE35_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU3061692.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1788..4199
                     /locus_tag="VVE35_001526"
     CDS             1788..4199
                     /locus_tag="VVE35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU3061693.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4449..5150
                     /locus_tag="VVE35_001527"
     CDS             4449..5150
                     /locus_tag="VVE35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061694.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5152..7860
                     /locus_tag="VVE35_001528"
     CDS             5152..7860
                     /locus_tag="VVE35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU3061695.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMETTANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     IVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATDQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNVSAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPSTI
                     LFYPQVGWEVYVIPVVAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7956..8447)
                     /gene="tpx"
                     /locus_tag="VVE35_001529"
     CDS             complement(7956..8447)
                     /gene="tpx"
                     /locus_tag="VVE35_001529"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU3061696.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8570..9499)
                     /gene="psaA"
                     /locus_tag="VVE35_001530"
     CDS             complement(8570..9499)
                     /gene="psaA"
                     /locus_tag="VVE35_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU3061697.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9531..10379)
                     /locus_tag="VVE35_001531"
     CDS             complement(9531..10379)
                     /locus_tag="VVE35_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU3061698.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10376..11098)
                     /locus_tag="VVE35_001532"
     CDS             complement(10376..11098)
                     /locus_tag="VVE35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU3061699.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKTKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3306 a         2071 c         2539 g         3300 t
ORIGIN      
        1 tttctgaaaa gtattatatt ttatttcata ttatacaaat ttttatttta taatatcaga
       61 aaatactttt tttaaaagca aatatgatac aattttattt gaaaaaaata aaaaaggaga
      121 ttttattatg aaattaaaaa cacttgcttt aattagtggt atcgtcggtc ttgtgggagg
      181 aattttactt cttattggtc cttttgtctt gttgggaaca gcggtaaaca cagctgctac
      241 aactcttaat ggaggagcta ctgcaggggc tttttcaggt gttgccttac tcttgaatgc
      301 cttgaagatt gcaaatcttg ttcttggtat cattgctatt gtttactata aaggagataa
      361 gcgtgtaggt gcagctccgt ctgtactaat gattgtttct ggtggagtta gtctcattcc
      421 attcttagga tgggttgggg ggattcttgc tattatcgga ggatctctat tccttgcaac
      481 attgaagaaa ttcaaatcag aagaataaaa ggtattttag catgaaaaga acaaaaaagt
      541 ttatcggtat aggagtagct ctattatctc tttcttttct agttgcatgt ggaacataaa
      601 gttcaaagaa tacttcaaca agtaatgatg agaagacagt agcaacatcc aatagttcaa
      661 aagaaacaat cactttcgat acaccggttg taacagacaa tgcgattgaa tcaatacgca
      721 cttatgcaga ttatatagat ctttataaaa atatttttga tgattatttt actaaagctg
      781 aggaagattt caaaggcaca gctatggaaa ataatgactc gtttactaaa ctaaaagagt
      841 caactcaaaa attattcgat gcgcagaaaa aaaggttaaa taatgaagat agaatagaaa
      901 caaccaaaaa caatgtgatt gccaaacatt gtcaaacagt cctttccttt ttggttttga
      961 ctagcttttt tgtgaaaaat tgtgtaaaat agaatagata aacgagggga aacctcggaa
     1021 aatttaaagg agaatccatc taatggtaaa attggttttt gctcgccacg gtgagtctga
     1081 atggaacaaa gctaaccttt tcactggttg ggctgatgtt gatttgtctg aaaaaggtac
     1141 acaacaagcg attgacgctg gtaaattgat caaagaagct ggtatcgaat ttgaccaagc
     1201 ttacacttca gtattgaaac gtgctatcaa aacaactaac ttggctcttg aagcttctga
     1261 ccaattgtgg gttccagttg aaaaatcatg gcgcttgaac gaacgtcact acggtggttt
     1321 gactggtaaa aacaaagctg aagctgctga acaatttggt gatgagcaag ttcacatctg
     1381 gcgtcgttca tacgatgtat tgcctccaaa catggaccgt gatgatgagc actcagctca
     1441 cacagaccgt cgttacgctt cacttgacga ctcagttatc ccagatgctg aaaacttgaa
     1501 agtgactttg gaacgtgctc ttccattctg ggaagataaa atcgctccag ctcttaaaga
     1561 tggtaaaaac gtattcgtag gagctcacgg taactcaatc cgtgcccttg taaaacacat
     1621 caaaggtttg tcagatgatg agatcatgga cgtggaaatc cctaacttcc caccattggt
     1681 attcgaattc gacgaaaaat tgaacgttgt ttctgaatac taccttggaa aataaaaaat
     1741 tgtaagccta ggattgattt ctaggctttt tatgttagta tggaagtatg ataaggaata
     1801 aaaaacaaga ttatgtactg gcctacaagc aaccagcttc aaccacttac atgggttggg
     1861 aagaagaagc tttaccgata ggtaatggtt ctttaggagc aaaagtattt ggccttatag
     1921 gggctgaacg gattcaattt aatgaaaaaa gtctctggtc tggaggtcca cttcctgata
     1981 gttcagatta tcagggtgga aatcttcagg atcagtatgt ttttttagct gagattcggc
     2041 aggctttgga gaagagagat tacaatctgg ctaaggaact ggctgagcag cacctaattg
     2101 ggccaaaaac gagtcaatat gggacctatc tgtcttttgg ggatattcac attgagttca
     2161 gccagcaagg tacgactttg tctcaggtga cggactatca gagacagttg aatattagta
     2221 aggcacttgc gacgacttct tatgtctata agggaacgcg atttgaacgt gaagcttttg
     2281 cgagttttcc agatgatctc ttggttcaat gttttactaa ggaagggttg gaaactctag
     2341 attttactat agaactatcc ttgacctgtg atttggcttc taatggaaag tatgagcagg
     2401 aaaaatctga ttacaaggag tgtaagttgg atattactga ttctcatatc ttgatgaagg
     2461 gaagagttaa ggataatgat ctgcggtttg ctagttatct agcttgggaa acggatggag
     2521 atattagagt ttggtcagat agggttcaga tatcaggagc cagttatgcc aatctcttct
     2581 tggccgctaa gacggatttt gcccaaaatc ctgctagcaa ttatcgcaag aaactagatt
     2641 tagagcaaca ggtgatagac ttggtggaca cagctaaaga aaagggctat acccaattga
     2701 aatcaaggca tatcgaggac taccaagcct tattccagcg tgttcaattg gatttggaag
     2761 ctgatgttga cgcatccact acagatgatt tgttaaaaaa ttataagcca caagaagggc
     2821 aggctttgga ggagctgttc ttccagtatg gacggtattt attgattagt tcgtccagag
     2881 actgcccaga tgctctacca gctaacctac agggagtctg gaatgcggtc gacaatcctc
     2941 cttggaattc ggactatcac ttaaatgtca atctgcagat gaattattgg ccagcctatg
     3001 ttaccaatct cctagagacg gtctttccag tcatcaacta tgtagatgat ttgcgtgtct
     3061 atggtcgtct agcggctgta aagtatgcag gaatcgtctc tcagaaaggt gaggagaatg
     3121 gttggttggt tcatactcaa gcgactccct ttggttggac ggcacctggt tgggattact
     3181 attggggttg gtcaccagct gccaatgcgt ggatgatgca aaccgtttat gaagcctatt
     3241 cattttatag ggaccaagac tatctcaggg agaaaattta tcccatgttg agggaaacgg
     3301 ttcgtttttg gaatgccttt ttacataagg atcagcaggc gcagcgttgg gtgtcttctc
     3361 cgtcttattc cccagaacat gggccgattt cgattggcaa tacctatgac caatctctga
     3421 tttggcagtt atttcatgat tttattcagg ctgctcagga attgggactg gatgaggact
     3481 tgttgactga ggttaaggag aagtctgatt tactaaatcc tttgcaaatc actcaatctg
     3541 gtcgaatcag ggagtggtat gaggaggaag agcagtattt tcaaaatgag aaagtggagg
     3601 cccagcatcg gcacgcttcc catctagtgg gactctatcc tggcaatctc tttagctaca
     3661 agggacaaga gtatattgaa gcggcacgtg ctagcctcaa tgatcgtgga gatggcggca
     3721 caggctggtc caaggctaat aagatcaatc tctgggcgcg tttgggagat ggcaatcgag
     3781 cccataaatt attggcagag cagttaaaaa catccacctt gccaaatctt tggtgtagcc
     3841 atcctccttt tcagatagat ggtaattttg gtgctactag tggcatggca gaaatgttac
     3901 tccagtctca tgcagcttat ctggtacctc tagctgccct acctgatgct tggtcaacag
     3961 gttctgtttc aggcttaatg gcacgtggac attttgaagt gagcatgagc tgggaagata
     4021 aaaaactctt acagttgacc attttatcaa ggagtggagg agatttgcga gtttcttatc
     4081 cagatattga gaagagtgtg attaaaatga atcaagaaaa aataaaagcg aaatgcatgg
     4141 ggaaagattg tatttcggtg gcaacagcag aaggtgatct tgttcaattt tatttttaag
     4201 aagatgttat aaggcagtaa tttgaaactg ccttttaata aggatttaag aatataggca
     4261 gtttccaact agttgaaaaa gcgttataat gataatagga agtaatactc aatgaaaatc
     4321 aaagagcaca aactaggaag ctagccgcag gttgctcaaa acagtgtttt gaggttgtag
     4381 atggaagctg acgtggtttg aagagagatt ttcgaggagt ataatttgtt tgatagaggg
     4441 tgggtctgat ggcttatatt gagatgaaac actgttacaa gcgttatcag gttggggaca
     4501 cggagattgt ggccaatcgt gatgtgaatt ttgagattga aaagggggag ctggttatta
     4561 tccttggtgc ttcaggtgca ggcaagtcaa cagttcttaa ccttcttggg ggaatggata
     4621 ccaatgatga aggggaaatc tggattgatg gtgttaatat tgcggattat agttcccacc
     4681 agcgcaccaa ttaccgtaga aatgatgtgg ggtttgtttt tcagttttat aatctagttt
     4741 ctaatctgac agctaaggaa aatgtggaac tggcttctga aattgtgaca gatgccttga
     4801 attctgatca ggtcttgaca gatgtaggtc tggctcatcg tctaaataac tttccagccc
     4861 agctttctgg aggggagcaa cagcgagtct ccattgcacg cgcggtagcc aaaaatccta
     4921 aaattcttct ttgtgatgaa ccgactggag ccttggatta tcagacgggc aagcaggttt
     4981 tgaaaattct ccaagatatg tctcgtcaaa agggagcgac ggtgatcatc gtgactcata
     5041 atggagcttt ggcgcccatt gctgatcgcg tgattcatat gcacgatgcc agtgtcaagg
     5101 atgtggtgct caaccagcat cctcaggata ttgacagttt ggagtactag catgatcaag
     5161 cgaaaaactt attggaagga cttaattcag tccttcacag gctccaaggg gcgtttttta
     5221 tccatcttga tcctgatgat gttgggatct ctagccttag taggcctcaa agtaaccagt
     5281 cccaacatgg agacgacagc taatgcttat ttaacaactg ctcaaacctt ggatttggca
     5341 gtcatgtcta actatggctt ggatcaagca gaccaagaag aactaaaaca gacggagggc
     5401 gcagaggtcg agtttggcta tttgacagat gtgactatgg ataatgggca ggatgccatt
     5461 cggctgtact ccaaaccaga gcgaatttca acctttcagc taagaaaggg acgacttcct
     5521 cagtcagaca aggaaatcgc tttggccact catttgcaag gccaatacag cgtgggacag
     5581 gagattagtt ttaaagaaaa agaagagggt cattcctctt taaaagacca tacttatacc
     5641 attactggtt ttgtggattc ggctgaaatc ctctcccagc gagatatggg ctacgcagga
     5701 agtggaagtg ggactctgac agcctatggg gtgattttac ctagtcaatt tgatcagaaa
     5761 gtctacaata tagctcgttt gaaatatcaa gatttagcag gtttaaatgc cttttcatca
     5821 gcttatgaag aaaaatccaa gcaacatcag gaagagcttg aacaaatttt atcagataat
     5881 ggcaaggtac gtctgcaact tttgaaaaaa gaaggacaag agtctctaga caaggggcaa
     5941 gagacccttg acaaggctca gactaatttg caggaaggca agcgtcgttt agcagctgct
     6001 caagctcgta tacaggctca agaaagtcaa ctagccttgt ttcctcaagt tcagagagag
     6061 caggctagtg ctcaacttac ccaagccaag caggaattgg gcaaggaaga ggccaaacta
     6121 aagcaagctg aacaaaatct agcccaagaa aaggaaaaat tagaaaaaca tcagcaagtc
     6181 ttggacgatt tggcggagcc aaggtatcag gtctataatc gtcagaccat gccaggtggt
     6241 cagggctatc ttatgtatag caatgcttca tccagtattc gagcagtggg caatatcttt
     6301 cctgtggtac tttatgccgt agcatccata gtgaccttta cgaccatgac tcgctttgta
     6361 gacgaagagc gaactcatgc agggattttt aaggccttgg gttaccgtag taaggatatt
     6421 atcaccaagt ttctccttta tggactagta gctgggactg tcggaacggc tctaggtagt
     6481 atacttggtc attatttgct agccagtgta atttcaagtg tcattacaaa aggcatggtg
     6541 gtgggagaaa cccagattca gttctattgg acctatagct tactagctct tgtcttgagt
     6601 tggttggcga gtgtgttacc agcctatctg gtggcttgga gggaacttca tgccgaagca
     6661 gcccagcttc tacttcctaa acctcctgtc aaaggagcta aaatcttatt ggagcgtatc
     6721 ggttttatct ggcgtcgtct cagttttact cataaggtaa cagcccgcaa catctttcgt
     6781 tataagcaga gaatgttgat gacaatcttt ggtgtggcag gttctgtagc tctgctcttt
     6841 gcaggtttgg gaatccaatc ttctgtagca ggagttccgt ctaaacagtt tcaacaaatc
     6901 caacagtatc agatgcttgt ctctgaaaat cctagtgcga ccgatcagga caaggtagag
     6961 ctagcagaag tgttgaaagg gcaggagata ctagcctacc agaaaatcta ttctaaaacg
     7021 ctagacaagg atttcaaagg caaggctggt cttcaaaaca ttactcttat gatgatagag
     7081 aaggaagatt tgactccctt tatccatctt caacatcatc agcaggagct gacattaaaa
     7141 gatggcatcg ttattacagc taaactcgcc cagctggcag gtgtcaaggt tgggcagact
     7201 ttagaaattg aaggtaagga actaaaggtc gctgctatta ctgagaacta cgttggtcac
     7261 tttatttata tgagtcaggc tagctatgag caactttacg gacagctacc ccaagccaac
     7321 acttatctgg tctcattaag ggataccagt gcaactagta tcgaaagtca ggcgggcttg
     7381 cttatgaatc aatctgcggt gtccagcgtt gtccaaaatg tttcagccat tcgactcttc
     7441 gactctatcg ctagctcact caatcagacc atgaccatct tggtcatcgt atcggttcta
     7501 ttagctattg tcatccttta caatttgacc aatatcaacg tagctgagag aatccgtgaa
     7561 ctctccacta tcaaggttct cggttttcat aataatgaag tcacactcta catttaccgt
     7621 gagacgattg tgctgtccct tgtgggaatc gtacttggtc tggtagctgg tttctattta
     7681 caccaatttt tgattcaaat gatttcgcct tcgactattc tcttttatcc gcaggtaggc
     7741 tgggaagtct atgtaatccc agtggtagca gtaagcatca ttttgacctt gcttggtttc
     7801 ttcgtcaatt attatctgag aaaggttgat atgttagaag ccctgaaatc tgtagagtaa
     7861 ggtagttatt tttagctgat tgaacttcta tttactaata ttcaaaaatc ctccgtttca
     7921 aagagcaggg aactctttgt gacagaggat tttttctata gggctttagc agctgcaatt
     7981 gcggcttcga agtttggctc agaattgata ttatccacgt attcaacgta gcgaatcgta
     8041 ttgtcagtat cgaggacaaa gactgcgcgt gctaataggt gccattcgtt gatcaagagg
     8101 gcataatcgt gcccgaaaga atggtcaaag tagtctgaaa gcataatggc attgtcaagg
     8161 ccttcagcac cgcaccaacg tttttgagca aaaggtaggt ccattgaaac agtcaatacg
     8221 accgtgttgt ccagtccagc caattcttca ttaaaacgac gtgtttgagt tgagcagatg
     8281 cctgtatcga tagaaggaac gacactcaag acttttttct tgccatcaaa atcagccaga
     8341 gattttttag aaagatctgt tgtagtaaga gaaaaatcaa gcgccttgtc gccgacttgt
     8401 agttgtttac ctgtaaagct cacaggattt ccgagaaaag ttaccatagg atactccaat
     8461 cttttttctt ccattgtatc tgaaacagtc agaattttcc aatgatttga ccggaaatgt
     8521 gggcatagaa aaaacgccag ctcacatgag aatgacgttt ttcagaggct tattttgcca
     8581 atccttcagc aatcttgtca aggttgtatt tcatcatgct gtagtagctg tcgccttctt
     8641 taccttgttc tgcgatagag tcagtaaaga tttgtgcgta gattgggatg tttgtgtctt
     8701 gagaaacagt tttcattgga cggtcatcca cacttgattc tacaaagagt gatggaactt
     8761 ttgtttggcg aagtttttca accaaggtct tgatttgttc aggagttcct tcttcttcag
     8821 tattgatttc ccagatgtag gcacttggga caccataggc tttagagaag tatttgaatg
     8881 ctccttcgct ggttacaatg agtttctttt cagcagggat cttattaaat ttatccttac
     8941 tttctttatc aagtttgtct aacttatcag tatattcttt gagatttttt tcatagaatt
     9001 ctttattgtt agggtctttg gcgctcaatt gtttggcgat atttttagca aaaataatac
     9061 cgttttcaag gttaagccaa gcgtgtgggt cttcttttcc tttttcattt tgaccttcaa
     9121 ggtagataac atcaacgccg tcgctgactg cgaagtagtc tttgttttca gttttcttgg
     9181 cattttctac caattttgta aaccaagcat tgccacctgt ttcaaggttg ataccgttat
     9241 agaaaatcaa atcagcctca gaagttttct taacgtcttc aggaagtggt tcgtattcgt
     9301 gtgggtcttg cccaatcgga acgatactat gaaggtcaat tttgtcacca gcaatatttt
     9361 tagtaatatc agcgatgatt gagtttgtag caacaacttt tagtttttga ccagaagttg
     9421 tatctttttt tccgctagca catgctacaa gaatgattgc agaaagaaag agaacgagta
     9481 atgtacctaa ttttttcatt agatcctcca atttattagg gctttgcccc ttattttaac
     9541 aaatgtttat ttttcagttt caaatatcgt tgtttgggag cgataaagaa gctaatgaga
     9601 aagaaactag cagctgtaag cacgatacta gaacctgccg caacattaaa actatagcca
     9661 ataaagagtc ccaaaactga agcagtagct ccgaaggttg aggaaaggaa aatcatgctt
     9721 ttcagactat tagcatacag ataagcagtt gcagctgggg taatcagcat ggctacaatc
     9781 aggatagttc cgacactttg catggctgtc acagacacga gagtcaggag taccatgaga
     9841 aggtagtgat agaaattgac aggcattccc atggctttag ccaagagttc atcaaaggaa
     9901 gttatcaaga gttgcttgaa gaaaatccag attaacaaga ggatggctgc ccccacaccc
     9961 atagtaataa acatatccgt atcttggacg gccaggatat taccaaaaag gatatggaaa
    10021 aggtcagttg aacttttagc gacaccaatc aagatgatac cgagggctaa gaaagaagaa
    10081 aaggtaatgc cgatggcggt atcgcttttg ataatcgagt ttcctttgat gtaggtaatg
    10141 atgatggcag ctagcaatcc aaagacaatg gctccgataa agaagtcaag gcccaagatg
    10201 aaggagaggg ctacacctgg taagacagca tgtgaaatgg catctcccat gagtgacatc
    10261 ccgcgtagaa taatgaaaca tcccacagct ccagctacaa tcccgacgac aatagctgtt
    10321 atcaaggcat tttgtaggaa atggaatttt tgcaatccat cgataaattc tgcaatcata
    10381 ggtcacctcc attgaaaaag agttgattac cgtaagcttc ttttagattg gtttcggtaa
    10441 aagtttcttt tgttggacca aaggcaatca cttctcgatt gacaagtaag acttgatcga
    10501 agtagtgggg aatcttgctg aggtcgtggt gaacgatgag aaccgtcttc ccagcttttt
    10561 tcaaatctct cagcgtattc atgatgattt cctcactgac agagtcaatc ccagcaaagg
    10621 gttcatccaa gaggatatag tcggcttcct gcaccaaaca tctggcaatc aaaacccgct
    10681 ggaattgacc tccagacagt tgactaattt gacgttcagc gtagtcagct aggccgacga
    10741 tttcaagggc ctcttgcact ttcttccaat gtttagtctt taaacttcga aagagaggaa
    10801 tagagggaaa tagtcctaac gagacgcatt ccttgacctt gatgggaaag ttgtagtcga
    10861 tattgatttt ttgttcgaca taggcaattc ggtgtaagga ttttttaact tccttgtcat
    10921 cgagaaatgc ctgaccttga tgtgggataa ttcccagcat accttttaat agtgttgatt
    10981 tcccagcgcc gtttggacca atgatgccgg taattgttgg tccatggagc actagtgaaa
    11041 tatccttaag tgccaacgtt tctttgtagg agacactgag gttttcgata cgtatcataa
    11101 acttgtattc ctcctgtctc ttaatataca ttaaaaaaaa aattaagtca agttaatttt
    11161 tgaaaaaatt aaaataataa ctgaaaaata gattctaaag ataactttca ggataa
//
LOCUS       DAVGJJ010000067        11193 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000067 DAVGJJ010000000
VERSION     DAVGJJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.067"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            321..734
                     /gene="rpsL"
                     /locus_tag="VVE35_001533"
     CDS             321..734
                     /gene="rpsL"
                     /locus_tag="VVE35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU3061700.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            754..1224
                     /gene="rpsG"
                     /locus_tag="VVE35_001534"
     CDS             754..1224
                     /gene="rpsG"
                     /locus_tag="VVE35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU3061701.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1649..3730
                     /gene="fusA"
                     /locus_tag="VVE35_001535"
     CDS             1649..3730
                     /gene="fusA"
                     /locus_tag="VVE35_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU3061702.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3834..8225
                     /locus_tag="VVE35_001536"
     CDS             3834..8225
                     /locus_tag="VVE35_001536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU3061703.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDVQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGYKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTLEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8327..8590
                     /locus_tag="VVE35_001537"
     CDS             8327..8590
                     /locus_tag="VVE35_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU3061704.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8583..8861
                     /locus_tag="VVE35_001538"
     CDS             8583..8861
                     /locus_tag="VVE35_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU3061705.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8882..9034
                     /locus_tag="VVE35_001539"
     CDS             8882..9034
                     /locus_tag="VVE35_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061706.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            9056..10297
                     /locus_tag="VVE35_001540"
     CDS             9056..10297
                     /locus_tag="VVE35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU3061707.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDLLWDQIFKTCRVYEEDPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAINAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(10351..>11193)
                     /locus_tag="VVE35_001541"
     CDS             complement(10351..>11193)
                     /locus_tag="VVE35_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU3061708.1"
                     /translation="SPGFLEQKQKIADTIIEFNEISEYVKDIPNKNQFIVKDITGKHA
                     HLAHFENTSQHDYKRDKHKKTLNQSNVYSTSLQVVRRASEEPIHYLCKYFGITATEES
                     LKQLEEIGENISRMENTIDNLYQREKQIKTDFNPPKFILKYFSKDLMKELDIHLPDIK
                     VDYTSYIFEYVSAGGNSSQKTTILFNGETVEATAEYIAKKIKSKQTAQAQRSLMTNKL
                     RTQIKERDHYTCQICAASTAEQSLLLLEIDHIVPVSKGGLSTPDNLQTLCWKCNRSKS
                     NKIL"
BASE COUNT         3302 a         2133 c         2587 g         3171 t
ORIGIN      
        1 tgagaagttt tctgtactat tttatatatc agtataaatg acggagataa aaatgtcaca
       61 taagccaagc cgtattatta ggaagatgat gtgttcccag ctatttttat tcagaaattg
      121 atctctaaca ttaccaataa aaaagaaaaa tatcttgaca agcaagggaa aattttgtta
      181 caatgataga cggtactttt tacttttggt ctctcaagag tgtacaggga cgtgctgaca
      241 aatgttgcaa aagtacacac agatgatatc tgtcaccaag tgtatcatca ccaaaaataa
      301 aaaaacacag gagaatgtag atgcctacaa ttaaccaatt ggttcgcaaa ccgcgtaaat
      361 caaaagtaga aaaatctaaa tcaccagctt tgaacgttgg ttacaatagt cataaaaaag
      421 ttcaaacaaa cgtttcttca ccacaaaaac gtggtgttgc aactcgtgtt ggaacaatga
      481 cacctaaaaa acctaactca gcccttcgta aattcgctcg tgtacgtttg agcaacctta
      541 tcgaagttac tgcctacatc ccaggtatcg gacacaactt gcaggagcac agcgtggtgc
      601 ttcttcgcgg tggacgtgta aaagaccttc caggggtacg ttaccatatc gtccgtggtg
      661 cacttgatac tgcaggtgtt aacgatcgta aacaaggccg ttctaaatac ggtactaaac
      721 gtccaaaagc ataaggaaag gggataaaga gaaatgagtc gtaaaaatag agctccaaaa
      781 cgtgacgtat tgccagatcc gctatacaat tcacaactag ttactcgtct tatcaaccgc
      841 gttatgcttg atggtaaacg tggtactgct gcttcaatcg tttacggtgc ttttgagcaa
      901 atcaaagaag ctactggcaa cgatgcactt gaagtatttg aaacagctat ggaaaacatc
      961 atgcctgtac ttgaagtacg tgcacgtcgt gttggtggtt ctaactacca agtcccagtt
     1021 gaagttcgtc cagaacgtcg tacaacactt ggacttcgtt ggttggtaac aatcgctcgt
     1081 cttcgtggtg aacacacaat gcaagaccgt cttgcaaaag aaatcttgga tgctgctaac
     1141 aacactggtg cagcagttaa gaaacgtgaa gatactcacc gtatggctga agctaaccgt
     1201 gcattcgcac acttccgttg gtaagatagg atgcgaaagc gttaagaaag tcccagagaa
     1261 aatagggaat cgaagcaggt tgcggttgca accaatgaga ttcatctttt tctccagact
     1321 tttagcttga gctcaactaa atcatgatgc taggaacggt aaggatgcaa ggtgaaaata
     1381 ggaaactgac gcagtattcg acgaatacaa ggagttttat ctttttcacg cagcatcccg
     1441 ttccagctca catcggctaa ctaactttag cccgggttca aattagctaa atcgattagt
     1501 attagctata actcagctta ccatctcgta agttgaaacc aacaatagca tgaaaacatt
     1561 gagaacgggt aggtcctgcc tatccgtttt tattaaaatc gtgttataat agaatagaaa
     1621 tcaaaaataa ataggagaaa caaacctcat ggcacgcgaa ttttcacttg aaaaaactcg
     1681 taatatcggt atcatggctc acgtcgatgc cggtaaaaca acaactactg agcgtattct
     1741 ttactacact ggtaaaatcc acaaaatcgg tgaaactcac gaaggtgcgt cacaaatgga
     1801 ctggatggag caagagcaag aacgtggtat cacgatcaca tctgctgcga caacagctca
     1861 atggaacaac caccgcgtaa acatcatcga cacaccagga cacgtggact tcacaatcga
     1921 agtacaacgt tctcttcgtg tattggatgg tgcggttacc gttcttgact cacaatcagg
     1981 tgttgagcct caaactgaaa cagtttggcg tcaagcaact gagtacggag ttccacgtat
     2041 cgtatttgcc aacaaaatgg acaaaatcgg tgctgacttc ctttactctg taagcacact
     2101 tcacgatcgc cttcaagcaa atgcacaccc aatccaattg ccaatcggtt ctgaagatga
     2161 cttccgtggt atcattgact tgatcaagat gaaagctgaa atctatacta acgaccttgg
     2221 tacggatatc cttgaagaag acatcccagc tgaatacctt gaccaagctc aagaataccg
     2281 tgaaaaattg attgaagcag ttgctgaaac tgacgaagaa ttgatgatga aatacctcga
     2341 aggtgaagaa atcactaacg aagaattgaa agctggtatc cgtaaagcga ctatcaacgt
     2401 tgaattcttc ccagtattgt gtggttcagc cttcaaaaac aaaggtgttc aattgatgct
     2461 tgatgcggtt atcgactacc ttccaagtcc acttgacatc ccagcaatca aaggtattaa
     2521 cccagataca gacgctgaag aaattcgtcc agcatctgac gaagagccat ttgcagctct
     2581 tgccttcaag atcatgactg acccattcgt aggtcgcttg acattcttcc gtgtttactc
     2641 aggtgttctt caatcaggtt catacgtatt gaatacttct aaaggtaaac gtgaacgtat
     2701 cggacgtatc cttcaaatgc acgctaacag ccgtcaagaa atcgacactg tttactcagg
     2761 tgatatcgct gctgccgttg gtttgaaaga tactacaact ggtgactcat tgacagatga
     2821 aaaagctaaa atcatccttg agtcaatcaa cgttccagaa ccagttatcc aattgatggt
     2881 tgagccaaaa tctaaagctg accaagacaa gatgggtatc gcccttcaaa aattggctga
     2941 agaagatcca acattccgcg ttgaaacaaa cgttgaaact ggtgaaacag ttatctcagg
     3001 tatgggtgaa cttcaccttg acgtccttgt tgatcgtatg cgtcgtgagt tcaaagttga
     3061 agcgaacgta ggtgctcctc aagtatctta ccgtgaaaca ttccgcgctt ctacccaagc
     3121 acgtggattc ttcaaacgtc agtctggtgg taaaggtcaa ttcggtgatg tatggattga
     3181 atttactcca aacgaagaag gtaaaggatt cgaattcgaa aacgcaatcg tcggtggtgt
     3241 ggttcctcgt gaatttatcc cagcggttga aaaaggtttg gtagaatcta tggctaacgg
     3301 tgttcttgca ggttacccaa tggttgacgt taaagctaag ctttatgatg gttcatatca
     3361 cgatgtcgac tcatctgaaa ctgccttcaa gattgcggct tcactttccc ttaaagaagc
     3421 tgctaaatca gcacaaccag ctatccttga accaatgatg cttgtaacaa tcactgttcc
     3481 agaagaaaac cttggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgtagatgg
     3541 tatggaagca cacggtaaca gccaaatcgt tcgtgcttac gttccacttg ctgaaatgtt
     3601 cggttacgca acagttcttc gttctgcatc tcaaggacgt ggtacattca tgatggtatt
     3661 tgaccactac gaagatgtac ctaagtcagt acaagaagaa attattaaga aaaataaagg
     3721 tgaagactaa tccgtcctca ctctagaagg aagtcactta gtggcttcct tttgtcttta
     3781 gaaaatacct ctaaatatgg taaaatagta gaagaataat gtgaggaaaa tgaatgtcaa
     3841 atagttttga aattttgatg aatcaattgg ggatgcctgc tgaaatgaga caggctcctg
     3901 ctttagcaca ggctaatatt gagcgagttg tggttcataa aattagtaag gtatgggagt
     3961 ttcatttcgt attttctaat attttaccta ttgaaatctt tttagaatta aagaaaggtt
     4021 tgagcgaaga attttctaag acaggcaata aagctgtttt tgaaattaag gctcggtctc
     4081 aagaattttc aaatcagctc ttgcagtcct actataggga ggctttctct gaaggtccat
     4141 gtgctagtca aggttttaag tccctttatc aaaatttgca agttcgtgct gagggcaatc
     4201 agctatttat tgaaggatct gaagcgattg ataaggaaca ttttaagaag aatcatcttc
     4261 ctaatttagc caaacaactt gaaaagtttg gttttccaac ttttaactgt caagtagaga
     4321 agaatgatgt cctgacccaa gagcaggaag aggcctttca tgctgaaaat gagcagattg
     4381 ttcaagctgc caatgaggaa gcgctccgtg ctatggaaca actggagcag atggcacctc
     4441 ctccagcgga agagaaacca gtctttgatt ttcaagcgaa aaaagctgca gctaaaccca
     4501 agctggataa ggcggagatt actcctatga tcgaagtgac gacagaggaa aatcgtctgg
     4561 tctttgaagg ggttgttttt gatgtggagc aaaaagtgac tagaacaggt cgtgttttaa
     4621 tcaactttaa aatgacggac tacacttcaa gtttttctat gcaaaagtgg gttaaaaacg
     4681 aggaagaggc ccagaagttt gacctcatca agaagaattc ttggctccga gttcgaggga
     4741 atgtggagat gaataacttc acacgcgatt tgactatgaa cgtacaggat gtgcaggaag
     4801 ttgttcacta tgagcggaag gatttgatgc cagaaggtga gcgtcgggtt gagtttcatg
     4861 ctcatactaa catgtcgact atggatgctt tgccagaggt cgaagagatt gttgcaacag
     4921 ctgctaagtg gggatacaag gcggttgcta tcacggacca tgggaatgtc cagtcctttc
     4981 cacatggcta taaggcggct aagaaagcgg gaatccagct gatctatggg atggaagcca
     5041 atatcgtgga ggaccgtgtc cctatcgtct ataacgaagt ggagatggac ttgtcagaag
     5101 caacctacgt ggtctttgac gtggaaacga cgggactttc agctatctat aatgacttga
     5161 ttcaggttgc ggcctctaag atgtacaagg ggaatgttat tgctgaattt gatgaattta
     5221 tcaatcctgg gcatcccttg tcagccttta ctacagagtt aactggaatt acagatgatc
     5281 atgtcaaaaa tgccaaacca ctagaacaag ttttgcaaga attccaagaa ttttgcaagg
     5341 atacggtcct agttgcccac aatgctacct ttgacgttgg ctttatgaat gctaattatg
     5401 agcgtcatgg tcttccaaag attagtcagc cagttattga tacgctggag tttgctagaa
     5461 acctctatcc tgagtataaa cgccatggtt tggggccttt gaccaagcgt tttggtgtgg
     5521 ccttggaaca tcaccacatg gccaactacg atgcggaagc gactggtcgt ctgcttttca
     5581 tctttatcaa agaggtagca gaaaaacatg gtgtgaccga tttagctaga ctcaacattg
     5641 atctaatcag tccagattct tacaaaaaag ctcggatcaa gcatgcgacc atctatgtca
     5701 agaatcaggt aggtctaaaa aatatcttta agctggtttc cttgtctaat accaagtatt
     5761 ttgaaggagt gccacggatt ccgagaacgg ttctagatgc ccatcgagag ggcttgattt
     5821 taggttcagc ctgttcagag ggtgaagttt ttgacgtggt cgtttctcaa ggtgtggatg
     5881 cggcggttga ggtggccaag tattatgact ttatcgaggt catgccaccg gctatctatg
     5941 cgcccttgat tgccaaagag caggtcaagg atatggagga actccagacc attatcaaga
     6001 gtttgataga ggttggagat cgccttggca agcctgttct ggctacggga aatgttcact
     6061 atatcgaacc ggaagaagag atttatcgtg aaattatcgt ccgtagtttg ggacagggtg
     6121 cgatgattaa ccgaactatc ggtcatggtg aacatgccca accagcgcca cttccaaagg
     6181 ctcattttcg aacgactaat gagatgttgg atgaatttgc ctttttggga gaggaactgg
     6241 ctcgtaaact ggttattgaa aacactaatg ccttggcaga aatatttgaa cccgttgaag
     6301 tcgttaaggg tgacttgtat acgcctttca tcgacaaggc tgaagaaaca gttgctgagt
     6361 tgacctataa gaaagctttt gagatttatg gaaatccgct gccagatatt gttgatttgc
     6421 ggattgaaaa agaattaaca tccatactgg ggaatggatt tgctgtgatt tatctggcat
     6481 cgcagatgct ggtgcaacgt tctaatgaac ggggttattt ggttggttct cgtgggtctg
     6541 tcggatctag tttcgttgcg accatgattg ggattacgga ggtcaatcct ctctctcctc
     6601 actatgtctg tggtcagtgt cagtacagtg agtttatcac agatggttcg tacggttcag
     6661 gatttgatat gccccataag gactgtccaa actgtggtca caaactcagt aaaaacggac
     6721 aggatattcc gtttgagacc ttccttggtt ttgatgggga taaggttcct gatattgact
     6781 tgaacttctc gggagaagat cagcctagcg cccacttgga tgtgcgtgat atctttggtg
     6841 aagaatatgc cttccgtgcg ggaacggttg gtacggtagc tgccaagact gcctatggat
     6901 ttgtcaaggg ttacgagcga gattatggca agttttatcg tgatgcagaa gtagaacgcc
     6961 tcgctcaagg agcggcgggt gtcaagcgga caacaggcca acacccgggg ggaatcgttg
     7021 ttattccgaa ctacatggat gtctacgatt ttacgcctgt ccagtatcca gcagatgatg
     7081 tcacggctga atggcagacc actcacttta acttccacga tatcgatgag aacgtcctca
     7141 aactcgatgt actgggacat gatgatccga ccatgattcg aaaacttcag gatttgtctg
     7201 gtattgaccc taataaaatt cctatggatg acgaaggcgt gatggcactc ttttctggga
     7261 ctgatgtact aggggtaaca cttgaacaaa ttggaacgcc tacgggtatg ttggggattc
     7321 cagagtttgg aacaaatttc gtacgtggaa tggtagacga aacccatccg acaacctttg
     7381 cggaattgct tcagctgtct ggtctgtccc acggtactga cgtttggttg gggaatgctc
     7441 aggatctgat taagcaagga atagcggacc tatcgactgt tatcggttgt cgggacgaca
     7501 tcatggttta cctcatgcat gcgggtctgg aacctaagat ggcctttacc attatggaac
     7561 gggtacgtaa gggtttgtgg ctaaagattt cagaagagga gagaaatggc tatatcgaag
     7621 ccatgaaggc taataaggtg ccagagtggt atatcgaatc ctgtgggaaa attaagtaca
     7681 tgttccctaa ggcccatgcg gcagcctacg ttatgatggc cttgcgtgta gcttacttca
     7741 aggttcacca tcctatttat tactactgtg cttacttctc cattcgtgct aaggcttttg
     7801 atatcaagac catgggtgcg ggcttggagg tcatcaagcg cagaatggaa gaaatctctg
     7861 aaaaacggaa gaacaatgaa gcctctaatg tggaaatcga tctctataca actcttgaga
     7921 ttgtcaatga gatgtgggaa cgaggtttca agtttggtaa attagatctc taccgtagtc
     7981 aggcgacaga gttcctcatc gacggggata cccttatccc accatttgta gcaatggatg
     8041 gtctgggaga gaacgttgcc aagcaactgg tgcgggcgcg tgaagaggga gaattcctct
     8101 ctaaaacaga actacgcaag cgtggtggac tctcatcaac cttggttgaa aagatggatg
     8161 agatgggtat tcttggaaat atgccagagg ataaccagtt gagtttgttt gatgagttgt
     8221 tttaaaaaat tgcttaataa tctattaaaa gaggctaacg tatatccaat agatttacat
     8281 tagctttctt ttttgttaaa atagtctatg gaaagagggt gagagtatgt caaagatgag
     8341 tataagcatc cgtctggata gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct
     8401 gggaatggat atgacaacag ctattaatat tttccttcgt caggcaattc aatatcaggg
     8461 attacctttt gatgttagac tagacgaaaa tcggaagttg ctccaagtgt taacggattt
     8521 agaccaaaat cgtaatatga gccagtcttt tgaatcagtc tcagatttga tggaggactt
     8581 acgtgcttaa gattcgttat cataaacagt ttaaaaaaga ttttaagttg gctatgaagc
     8641 gtggtttgaa ggcagaatta ttagaagaag ttttgaattt tctggttcaa gaaaaagaac
     8701 atcctgccag atatcgtgat cattcattga cggcatccaa gcattttcaa ggagttcgtg
     8761 aatgccatac ccagccagat tggcttttgg tttataaagt agacaagtcg gaattgattt
     8821 taaatttgct gaggacaggc agtcacagtg atttatttta atctatttta agggggttct
     8881 catgaaacta agaatatttg cggaagataa gccggctaag aaggtatttg aatatcaatt
     8941 agaacttgct gatcgtacaa ttcttctatc gacagcactc ttgtcaggtg ctattgcttt
     9001 agcaggaatc ttttctgcct tgaaagaaaa ataaaaatag aaaagagaaa acagaatggt
     9061 tttaccaaat tttaaagaaa atctagaaaa atatgcgaaa ttgttggttg cgaacggaat
     9121 taacgtgcaa cctggtcaca ctttggctct ctctattgat gtggagcaac gtgaattggc
     9181 acatctaatc gtgaaagaag cttatgcctt gggtgcgcat gaggttattg ttcagtggac
     9241 agatgatgtg attaaccgtg agaaatttct ccatgcgccg atggagcgtc tggacaatgt
     9301 accagaatac aagattgctg agatgaacta tctcttggag aacaaggcta gtcgtcttgg
     9361 tgtccgttct tctgacccag gtgctttgaa cggagtcgat gctgacaagc tttcagcttc
     9421 tgccaaagct atgggacttg ctatgaagcc aatgcgtatc gcaactcagt ctaacaaggt
     9481 tagctggaca gtggctgccg ctgctggact agagtgggct aagaaggtct tcccaaatgc
     9541 tgcgagcgac gaagaagcag ttgatctcct ttgggaccaa atcttcaaaa cttgccgtgt
     9601 ctacgaagaa gatcccgtta aggcttggga agaacatgca gctatcttaa agagcaaggc
     9661 ggatatgctc aataaagagc aattttcagc ccttcactac acagcgccag gaacagattt
     9721 gacacttggt ttgccaaaga accacgtttg ggaatcagct ggtgctatca atgcacaggg
     9781 cgaaggattc ttgccaaata tgccgacaga ggaagtcttc acagcacctg acttccgtcg
     9841 cgcagatggt tatgtcactt ctacaaaacc gcttagctat aacggaaata tcattgaagg
     9901 tattaaggta acctttaagg atggacaaat tgtggatatc actgcagaaa aaggcgatca
     9961 agtcatgaaa gaccttgtct ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt
    10021 ggtaccagat ccaagtccaa tttctcagtc aggcattacc ttctttaaca cccttttcga
    10081 tgaaaatgcg tcaaaccact tggctatcgg tgcagcctat gcgactagcg ttgttgatgg
    10141 agcggagatg agcgaagagg agcttgaagc tgcagggctt aaccgttcag atgttcacgt
    10201 agactttatg attggttcta accaaatgga tatcgatggt attcgtgagg atggaacgcg
    10261 agtacctctt ttccgtaatg ggaattgggc aaattagcaa aaatatccca aaagcaaatt
    10321 gaattgtaag tatgcttttg ggatatttat ttataagatt ttattagatt ttgaacgatt
    10381 acatttccaa cagagagttt gaagattgtc aggtgtggaa agtcctcctt ttgatacagg
    10441 gacaatatgg tctatctcaa gtaacaggag agattgttca gctgtagagg cagcacagat
    10501 ttgacaagta tagtggtctc tttcttttat ttgagttcga agtttgtttg tcatcagtga
    10561 tcgttgagct tgagcagttt gttttgattt tattttcttt gcaatatact ctgctgttgc
    10621 ttcaacagtt tcaccgttaa acagaattgt cgttttctga gaactattgc ctcctgcact
    10681 cacgtattca aaaatataag aagtgtaatc aacctttatg tctggtaggt ggatatctaa
    10741 ctctttcatt aaatctttag aaaagtattt taaaataaat tttggaggat taaagtcggt
    10801 ttttatttgt ttctctcgtt gataaagatt atcaattgtg ttttccattc gagagatatt
    10861 ctctccgatt tcttcaagct gtttcaagct ttcttcggtt gcagttattc cgaaatactt
    10921 acagagataa tgtataggtt cttcagatgc acgtcgcaca acttgtaggg aagttgaata
    10981 aacgttactt tgatttaaag ttttcttatg tttatctcgt ttatagtcat gttggctagt
    11041 attttcaaag tgagctaaat gtgcatgttt cccagtaata tcctttacaa taaattgatt
    11101 tttatttggg atatctttta catattctga aatttcatta aactcaataa tagtatctgc
    11161 aattttttgt ttttgttcta gaaaacctgg act
//
LOCUS       DAVGJJ010000068        10828 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000068 DAVGJJ010000000
VERSION     DAVGJJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.068"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..493
                     /locus_tag="VVE35_001542"
                     /pseudo
     CDS             1..493
                     /locus_tag="VVE35_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            593..802
                     /locus_tag="VVE35_001543"
                     /pseudo
     CDS             593..802
                     /locus_tag="VVE35_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            702..1148
                     /locus_tag="VVE35_001544"
     CDS             702..1148
                     /locus_tag="VVE35_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3061709.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFYPALVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1286..2590)
                     /gene="eno"
                     /locus_tag="VVE35_001545"
     CDS             complement(1286..2590)
                     /gene="eno"
                     /locus_tag="VVE35_001545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU3061710.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2754..3200
                     /locus_tag="VVE35_001546"
     CDS             2754..3200
                     /locus_tag="VVE35_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HEU3061711.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPLGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3197..4312)
                     /locus_tag="VVE35_001547"
     CDS             complement(3197..4312)
                     /locus_tag="VVE35_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU3061712.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4321..4966)
                     /gene="serB"
                     /locus_tag="VVE35_001548"
                     /pseudo
     CDS             complement(4321..4966)
                     /gene="serB"
                     /locus_tag="VVE35_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(5025..6458)
                     /gene="glgA"
                     /locus_tag="VVE35_001549"
     CDS             complement(5025..6458)
                     /gene="glgA"
                     /locus_tag="VVE35_001549"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU3061713.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6455..7594)
                     /gene="glgD"
                     /locus_tag="VVE35_001550"
     CDS             complement(6455..7594)
                     /gene="glgD"
                     /locus_tag="VVE35_001550"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU3061714.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKFFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7584..8726)
                     /locus_tag="VVE35_001551"
     CDS             complement(7584..8726)
                     /locus_tag="VVE35_001551"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU3061715.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8716..10644)
                     /gene="glgB"
                     /locus_tag="VVE35_001552"
     CDS             complement(8716..10644)
                     /gene="glgB"
                     /locus_tag="VVE35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU3061716.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTYNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     EPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3324 a         2369 c         1907 g         3228 t
ORIGIN      
        1 aaaaggaaca aaaccaagaa aggttgatag agatagactt aaaaactatc ttactgacaa
       61 tccagacgct tatttgactg aaatagcttc tgaatttggc tgtcatccaa ctaccatcca
      121 ctatgcactc aaagctatgg gctacactcg aaaaaaaaga accacaccta ctatgaataa
      181 gacccagaaa aagtagcctt atttcttaag aattttaata gtttaaagca cctagcacct
      241 gtttagattg acgaaacagg attcgatact tatttttatc gagaatatgg tcgctcatta
      301 aaaggtcagt taagaagagg caaagtatct ggaagaagat atcagagaat ttctttggtt
      361 gcaggtctaa caaatgaatg gtgagttaat cgctccaatg acttacgaag agacgatgac
      421 gagcgacttt tttgaagctt ggtttcagaa gtttctctta ccaacattaa acacaccatc
      481 ggttattata gtaaaatgaa ataagaatag gacaaatcga tcaggacagt aaaatcgaat
      541 tctaacaatg ttttagaagt agaggtgtac tattttagtt tcaatctact atattatgga
      601 taatgcaaga ttccatagaa tggggaagtt agaactttta tgcgaagagt ttgggcataa
      661 acttttacct cttcctccct actcacctga gtacaatcct attgagaaaa catgggctca
      721 tatcaaaaag cacctcaaaa aggtattacc aagttgcaat accttttacg aggctttttt
      781 atcctgctct tgtttcaatt gactatataa attgtctaag cgaaacaacc gataagaatt
      841 ggcacaaaag cgaccgtatt tttgttacca atacaggaaa accagttcat agttctatct
      901 tgagcaagtc tctccagcga gcaaacgaac gccttaaaaa accaattccc aaacatctgt
      961 cccctcacat cttcagacac accactatta gcatcttatc agaaaataaa attcctttaa
     1021 aaacaatcac ggacagggtt ggtcattccg actctgaagt cactacttcc atctacacac
     1081 acgtcacaaa gaacatgaaa gatgaagcaa tcaatgtact ggataaagtt atgaaaaaga
     1141 ttttttaaaa agttttgccc cttttttgtc ctctaaatac aaaaatagcc cttcggataa
     1201 aatccgaggg gctagaaacg ttgttaaatc aacggccgaa cttttgaact tcatggttcg
     1261 ggacaaaata gttcactgaa ctattttatt ttttaaggtt atagaatgat ttcaatccac
     1321 gatattcagc tacttcacca agttggtctt cgatacgaag caattggttg tatttagcga
     1381 tgcggtctgt acgtgaaagt gaaccagtct tgatttgtcc tgcgttagtt gcaaccgcaa
     1441 tatcagcgat tgttgaatct tcagtttcac ctgaacggtg tgatacaaca gcagtgtaac
     1501 cagcttcttt agccatttcg atagcttcaa aagtttcagt aagagtaccg atttggttaa
     1561 ctttgataag gattgagtta gcagcacctt cttggatacc acgtgcaagg tagtcagtgt
     1621 ttgttacgaa gaagtcgtca ccaacaagtt gtactttctt accaagacgt tcagtaagag
     1681 ctttccaacc atcccagtcg ttttcatcca taccatcttc aatagtgatg attgggtatt
     1741 tgttaaccaa ttcttcaagg tagtcgattt gttctgcaga tgtacgaaca gcagcacctt
     1801 caccttcaaa tttagtgtag tcgtaaactt tacgttcttt atcgtagaat tctgatgaag
     1861 cacagtcaaa tccgataaat acgtctttac ctggtacata tccagcagct tcaatcgcag
     1921 caaggatagt ttcaacacca tcttcagttc cttcgaaacg aggagcgaat ccaccttcgt
     1981 cacctacggc agtttccaaa ccacgtgatt taaggatttt cttaagagcg tggaagattt
     2041 cagcaccgta acgaagggct tctttaaatg ttggcgcacc aactggcaag atcatgaact
     2101 cttggaaagc gattggagcg tcagagtgag aaccaccgtt gatgatgttc atcattggag
     2161 ttggaagaac tttagtgttg aatccaccaa ggtagctgta aagtgggatt tcaaggtagt
     2221 cagcagcagc acgagctaca gcgatagaca caccgaggat tgcattcgca cccaatttac
     2281 ctttgttagg agtaccgtca agtgcgatca tagcacggtc aatagcttgt tgatcacgta
     2341 catcgtagcc aatgatagct tcagcaatga tgttgtttac gttgtcaaca gctttttgtg
     2401 taccaagacc accgtaacga gatttgtcac cgtcgcgaag ttcaactgct tcgtgttcac
     2461 cagtagaagc tcctgatgga accataccac gtccgaaagc acctgattca gtgtaaactt
     2521 ctacttcaag tgttgggtta ccgcgtgagt ctaggacttc gcgagcgtaa acatcagtaa
     2581 taattgacat tttttactct ccttatgagt taaatttttt acacctctat aataccttaa
     2641 aacccctcct ttttcaagaa aaaacgttat ctttgtgcaa cttttcctta actttataaa
     2701 gtaatcgctt tcttttgtct gttttattct aacttttatg atatactgtt ttcatgacag
     2761 atttatcaaa acaattactt gaaaaagctc atggtgggtt aaaaataaat ccggatgagc
     2821 aaagacgcta tcttggtact tttgaggaaa gagttcttgg atatgtagat attgacacag
     2881 caaatagccc tcagttagaa aaaggctttt tatttatttt agaaaacctt caggaaaaag
     2941 caaagccact atttgtgaag atttcaccaa ctatcgaatt tgataagcaa gttttctact
     3001 taaaagaagc aaaagaaact gatagtcaag ccaccatagt atctgaagag catattactt
     3061 ctcctttggg cctggttatt catagcaatg caccagttca agtagaagaa aaagaccttc
     3121 gacttgcttt tccaaaactt tgggaagtta aaaaggaaga accagccaaa acatccttat
     3181 ggaagaaatg gtttagctaa atcttgcaca tatttaataa gtgcccaata ttggcagccg
     3241 tgcgctccag atagagactg gcatttttca aactatcttc taaaggttca cttttctcca
     3301 aaatagaaaa gacagcttgg atattttcaa atggtagggg aggtaaatct tcagcaagac
     3361 taccgcaaat agcaataaca ggaactccaa caggggttct ttttgcaaca cctataggcg
     3421 ctttcccagc aaagctttga ctatcaagtc ttccttctcc aacaacaacc aagtcagcat
     3481 ctgaaacttt cttatcaaag ttgattaagt ccaagcaggt atcaattcca gacacgatac
     3541 ttgcctgagc aaaggcacac aaaccaccag caaggcctcc acctgctcct gctcctttaa
     3601 tttctaatgt tgcaggtgag aatttttcat aaaaatcttg gatcgcctga tctacgactg
     3661 caaacatagt cggatgtaga cctttttgat tgccaaaagt gtaagtcgca ccttgatgac
     3721 cacataaggg actcacgaca tctgctaaaa tatgaatttg aacaccttca ggaattttat
     3781 agcaattttc tgttgaaaca gaagctaagt ttaataagga ttgaccggaa gcaggcaaga
     3841 catttccatc cctatcataa aattgataac ctaaaccagc agcaatcccc agtcctccat
     3901 cattactggc cgtgccacca acaccgatat aaatatcttt aatcccttta gaaatgagat
     3961 ggagaatcaa ctctccaata ccacaagttt ggatttgaag tggatttcgt ttctctagcg
     4021 gaatttttcc aagaccaacc aagtcagcta cttcaaatag tgccagttcc cctttttgaa
     4081 aatagcgcat ggcttctttt tgtccaaaag ggtctgtcac ttggatccat ttttctttta
     4141 ggtcaagaga atgtcggata gcatctacag taccttctcc cccatcacca acagggcaga
     4201 ggagacattc tacatctgct atcgattgtt ggaagcctct ttttattgct tcagctacct
     4261 gttgagctgt caagctttcc ttaaacgaat ccggtgcaat tacaatcttc atattttccc
     4321 tcattctaaa cagtcaatca aaggaagaac ttctaaaaaa tccctcttgt caacatgatg
     4381 tggtatttct tttttgagca cttctttggc acaaaaggcg attcctaact ccgccgactt
     4441 caacattaat agattattga ccccatcacc gattgccacc attctttctt tagaaagttt
     4501 tagtttcttt ctccattttt ccagagtttc ttttttgacc tggggactta taatttgtcc
     4561 aactaatttt cctgttaaaa gaccttcttt gacttcaagc tggttggcag tgaaataggc
     4621 aataccaagg gattttgcta atctctccaa ctattggtgt aaatccacca gacaccagac
     4681 caactaggat gccattcttt tggagaatag agatgaactc tgggacattt agctatagat
     4741 gaattgagtt gaagacgtta tcaaagacca aaataggaag accttccaac aaggacactc
     4801 tttttcttaa actgctttca aagaccaact ctcctcgcat tgctcgactt gtaatctgcg
     4861 aaatttccgc ctcatgacct gcctctctcc ctaaaagatc aatcacttct tctaggatta
     4921 aggttccatc tacatccaaa acacacaagc cttttacttg agacatcagt tctcctctct
     4981 aaacagccta aaaatcgtat gaagtcatca tacgatttta tctattaatt aactaaacta
     5041 tggtacaagt caaggtatga cttgcaggct gtatcccatg agaagtcact ctccatagct
     5101 tgtttttgta ggtttctcca aatgtctgga tggtttctat acaagtccaa tgctgtttgg
     5161 aaagtccaat ttaaccaata aggagataga ttgtcaaagc taaagccagt accgcttcct
     5221 tcgattggat tgaaagcgcg aactgtatct cgcaagcctc caacttcatg gaccaatggc
     5281 aaggttccat aacgcatagc catcatttga gacaagccac acggttcaaa acgacttggc
     5341 atgaggaaga ggtcacaagc agcgtagatt tcctgagcaa gtttgacatc aaaagtgata
     5401 tttgttgata gcttgtctgg ataaatctga gcaaaccatg agaaagctcc ttcaaaggct
     5461 ggatcgccag ttcccaaaag aacaatctga acatcttctt gcaagatatg gtgaagactt
     5521 tcgaccacca catcaaaacc tttttgacgt gtcaaacgag aaacaattcc caccagtgga
     5581 acgtctgctc taacaggcaa gccaactctt tcttgcaatt ttgccttatt tttggctttc
     5641 ccagacaaat cttcctgatt gaaatgatag tctaaaagag catccgtctg aggattataa
     5701 agatcagcat caatcccatt cacgatacca gatactttac cagactccat tttaagaatc
     5761 tgatccaaat tacatccaaa ctgactagtc ataatttcat gagcatagct aggtgaaacg
     5821 gttgaaacac ggttcgcata gagaatacct gccttcatcc agttcagaca gttgttccat
     5881 cgaagggtgc catcagcgta acgttcaaag ccaactccaa acaaatcacc caacattcct
     5941 tctgaaaatt gtccttggaa ttctaaatta tgaatggtta aaactgtttc aatgtcctca
     6001 taggcttgaa tccaacggta tttttccttc aacaagaaag gaatcatagc tgtatggtag
     6061 tcatgaacat ggagaagatc aggaataaag tcaatccttt ccatagcctc aatggcagcc
     6121 agttggaaaa aggcaaagcg ttctccgtca tcaaaatcac cgtaaacatg accacggaag
     6181 aaataatatt gattgtcaat aaagtagaag gttacaccat ttaatactgt tttcttaatt
     6241 ccacaatact gtctgcgcca accaacgctc acctcaaaat gaagcacatc ttcaatctga
     6301 tttccaaatt tagcctctac catatcatag tagggtaaaa tcactgcaac ttcgtgccca
     6361 gcttttacca gtgattttgg aagagcgcca atgacgtctc ccaaaccacc tgtttttgaa
     6421 aagggtgcac cctctgctgc tacaaataaa attttcatga atgaatatcc tctgttactt
     6481 tagcaccttt cttaaccaca actggatgtt ctgcagttcc tcgaatcaca acaccatgct
     6541 caacttcaac ccctttgtcc aagatagcat attcgacctg agccccttct ccaataacaa
     6601 cacgagggaa gagcaggcta tctttaacca agctatcctt atggacatga atattacgtg
     6661 atagaacaga attagctact tgaccttcaa taatactacc agaggcaaac tgagaagtgc
     6721 ttaccttaga tgtattagca tagtaagttg gctcttcgtt tttgaccttt gtataaatct
     6781 tttggtttgg tgagaaaaga gaatagaatt tttgtgattc aagcatatcg atattcgctt
     6841 gataataaga tttaacagag tgaatattgg ctagatagcc cgtgtactcg taggcgaaag
     6901 ctccctcttt tacagccaaa tcccgtaaaa catagcgcaa tttctctgga tgttcttttt
     6961 tagcttcttc ttccaagtgt tcaatcaacc aaggtgtatc aacgacaaag atatctgtag
     7021 acatattgaa cgtttcagct gttgacttgc tatcaaagaa tttatgagaa agaacatggt
     7081 ctgtttcatc tacatccaag attgcattta cttctgaaat atctttctta gctagttttt
     7141 tataaactac agtgataggc tcttttgttg tactatgtag gtggaaaact tggttcaaat
     7201 caatgttaat aagaacatcg cagttgaggg caaccgtttg gtttgagcca gaacgtttca
     7261 aataagtaag aagctgttgg tagtattctt ttccaactgt actactttct acacgggtat
     7321 tgtaaattcc tagatagtaa tggctaagaa gggttgataa gccccactcg cgtcctgaac
     7381 gaatatggtc aaatactgag ctgatattat cctgctggaa aataccaaag acactacgaa
     7441 cacctgcatt agcaaggctt gaaagtggga agtcaatcaa acgatatttc ccaccaaatg
     7501 gcaaacttgc tactggacgg tggtccgtca atgtcgacat attgtgaaaa ccaactgtat
     7561 ttcctaaaat ggcagaatat ttatcaatct tcatctgttg ctacccccac tacttcatta
     7621 tatcctacaa cttgtacttc atctgttcca tcaatttcga caccgtcaga aataatcgca
     7681 ccttcaccaa taatggcacg tttaatctta gctccttgac caatgatagc tccactcatg
     7741 ataactgaat caaggacttc cgctccttcg cgaacttgcg cgcctgttga aaggatagaa
     7801 tgtttaacag ttccatcaac gaaacatccg tctacaacta atgagtcttc cacatgagca
     7861 tttgccccga ggaagtttgg tggtgaaatc aagtttcttg agtaaatctt ccattgacgg
     7921 ttacgactat ccaaggcatt ttctggagaa atatactcca tgttcgcttc ccaaagtgac
     7981 tcaatagtac caacatcttt ccaataacca ctaaattcgt aagcataaac actttcacct
     8041 gactcaaggt aatttggaat gacattttta ccaaagtctg acatgccaac cttgctcttt
     8101 tcagcagcga ctaacatatt acgaaggcgt tgccaatcaa aaatgtagat tcccatagaa
     8161 gcttttgtag atttaggttg agctggtttt tcttcaaatt caacaatacg attgttagca
     8221 tctgtgttca tgataccaaa acggcttgct tctttaagag ggacgtctaa aactgctact
     8281 gtcaagctgg cattattatc cttatgagac tggagcatat catcatagtc cattttgtag
     8341 atgtggtccc cagacaaaat caagacatac tcaggattga cactgtcgat atagtcgata
     8401 ttttggtaaa tagcgtgact agtcccctca aaccaacgat ttccttcact tgcagaataa
     8461 ggttgaagaa tagagacacc tgaattaata ccgtctagtc cccagcttga accattccca
     8521 atatggttgt tgagagcaag tggttgatac tgtgtaacga ccccaacatt atgaatccct
     8581 gagttggcac agtttgatag ggcaaagtca atgatacggt agcgcccacc aaattgcaca
     8641 gctggttttg cgatgctttg agtgagttta ccgagacgag ttccttgccc accagcaaga
     8701 atcaaagcta acatttcatt tttcattttc tactcctttt tggtttttat ttgtgacggt
     8761 tttagtagat ttcaagcgac gtttgatttt ccatacactt gctcccatag ccggtagggt
     8821 aaaggttaag gtctgctcat aatctttcca tagtccttct tgcgtttgaa cagtttgatt
     8881 atgttctttc caaacgcctc cccactcttc caactcagta ttccatactt cttcgtaaat
     8941 tcctgcaacg ggtagtccga ttgtaaaatc tttccgctca acaggtacca tgttaaagat
     9001 acagactaac atttctccct ttttaccctt acgaataaag gaaagaacac tctggtctcg
     9061 attatccgca tcaatgattt caataccatc atagctggta tcgatttccc acagacagcg
     9121 atgatctttg taaaactggt ttagctgaga agtgaaatac ttcatcttag cattcattgg
     9181 gtcttctagg ttagaccatt ccaactgttc ttcagatttc cattctagga attgaccgta
     9241 ttcgctaccc atgaagagca atttcttacc agggtgacaa atttggtacg tatagagatt
     9301 gcgcaagcct gcgaattgat tgtaacgatc tccccacatc ttatgcatca tactcttctt
     9361 gccatgaacc acttcatcgt gcgagaatgg caagagataa ttctccttga aaacatacat
     9421 aaagctgaaa gtcaccaggt taaagtcata tttacgataa atcggctctt cttcgtagaa
     9481 acggaggata tcattcatcc agcccatgtt ccatttgtag tcaaatccta gaccaccaat
     9541 ctctttcatt cccgtaatct tggttgcaga cgaactttct tctgcaatca tcatcacatc
     9601 tggatattct aacttaataa cctcattcaa gcgctgaagg aaataataac cttcatagtt
     9661 gagatttccg ccatctttat taggtgtcca tggagcatca tcatagtcca aatagagcat
     9721 gttgctaaca gcatccacac gaataccatc caaatgatag acatcaatcc aatgcttaat
     9781 gcaagaaatt aagaaggact ggacttcatt ttttccaagg tcaaaattaa gggcacccca
     9841 accatggtta tgagccttat tatggtcttg gtattcaaaa gtcggtgtcc catcataata
     9901 ggctaaggca tcatcgttga tggtaaagtg acctggtacc cagtccacaa taaccccaat
     9961 attataggta tgacactcct cgacaaaatc ttgaaactcc tctggtcggc cataagcatg
    10021 ctctaaagcg aagtaaccca taagctgata cccccaactc aagcccaaag gatgggacat
    10081 caagggcata aactcaatat gagtatagtt catttcaacg agataaggaa tgagttcatc
    10141 cttgagctgg gcaaaactat aaggactgcc atcagaattt cttttccatg atccagcgtg
    10201 aacttcataa atattgacag gacgctcttc aaagccccaa cgttttcttc gtgccagcca
    10261 aagtccatcc ttccatttct tctcaggaag ctctgttaag attgcccctg ttcctggacg
    10321 agcctcatac ctgacggcaa aagggtcaat cttcatcagt tgatgaccat tttgacgtgt
    10381 gacatgatat ttgtaaatat gcccttcttg agccatattg gtaaagactt cccagacccc
    10441 aaagtcattt cttaccattg gaatctgatt ttcaatccaa ttggtaaaat caccaaccaa
    10501 gtgaacagcc tgagcattag gtgcccaaac acggaaggta tagccatact ctccatttag
    10561 ttcttcccta tgtgctccta gataatgttg gagataaaaa ttttcacccg tcataaaggt
    10621 ttttaatgct tctctattat ccatatactc cctttctcct gtaagcgttt tctatgtttt
    10681 tattatacta cctttttaga gaagattcaa gtaaattact atacttcttt aattattttg
    10741 aaaatctaca acaagttcac ttactcgttc aattgtaaat caatattttt tcaaaaaatt
    10801 gcgaaaacac ctttcttttt ctactata
//
LOCUS       DAVGJJ010000069        10806 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000069 DAVGJJ010000000
VERSION     DAVGJJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.069"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..348
                     /locus_tag="VVE35_001553"
     CDS             <1..348
                     /locus_tag="VVE35_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU3061717.1"
                     /translation="KSMLEQAFTEKYYENTILHSDQGWQYRHDSYHRFLESKGVQASM
                     SRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSLKQLEQAIIDYIDYYNNKRIKIKLK
                     GLSLVQYRTKSFG"
     gene            complement(464..1267)
                     /locus_tag="VVE35_001554"
     CDS             complement(464..1267)
                     /locus_tag="VVE35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU3061718.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1544..2299)
                     /locus_tag="VVE35_001555"
     CDS             complement(1544..2299)
                     /locus_tag="VVE35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU3061719.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2369..5020)
                     /locus_tag="VVE35_001556"
     CDS             complement(2369..5020)
                     /locus_tag="VVE35_001556"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU3061720.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRVLEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNDLAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(5013..5591)
                     /locus_tag="VVE35_001557"
     CDS             complement(5013..5591)
                     /locus_tag="VVE35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU3061721.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5591..6151)
                     /locus_tag="VVE35_001558"
     CDS             complement(5591..6151)
                     /locus_tag="VVE35_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061722.1"
                     /translation="MARTELSDKIETERLVLRVRTVADAEDIHAYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIAGSVDFNHRHEDDVLEIGY
                     TLPPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEVI"
     gene            complement(6138..6587)
                     /locus_tag="VVE35_001559"
     CDS             complement(6138..6587)
                     /locus_tag="VVE35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU3061723.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQISILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPEFLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7000..7290)
                     /locus_tag="VVE35_001560"
     CDS             complement(7000..7290)
                     /locus_tag="VVE35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU3061724.1"
                     /translation="MKRAEQSASAAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANL
                     GGLPLDRAKTFYDEGIKSASDFKNWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7490..7744)
                     /locus_tag="VVE35_001561"
     CDS             complement(7490..7744)
                     /locus_tag="VVE35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU3061725.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7754..9088)
                     /locus_tag="VVE35_001562"
     CDS             complement(7754..9088)
                     /locus_tag="VVE35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU3061726.1"
                     /translation="MTDERIHVLRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9088..9321)
                     /locus_tag="VVE35_001563"
     CDS             complement(9088..9321)
                     /locus_tag="VVE35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU3061727.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            9352..9429
                     /locus_tag="VVE35_001564"
                     /pseudo
     CDS             9352..9429
                     /locus_tag="VVE35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9725..9907)
                     /locus_tag="VVE35_001565"
     CDS             complement(9725..9907)
                     /locus_tag="VVE35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061728.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9909..10688)
                     /locus_tag="VVE35_001566"
     CDS             complement(9909..10688)
                     /locus_tag="VVE35_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061729.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         3125 a         2416 c         2034 g         3231 t
ORIGIN      
        1 aagagtatgt tagaacaggc ctttacagag aaatactatg agaatacgat tctccatagt
       61 gaccaaggct ggcaataccg acacgattct tatcatcggt tcctagagag taagggagtt
      121 caagcatcca tgtcacgtaa gggtaacagc ccagacaatg gtatgatgga gtccttcttt
      181 gggattctga aatcggaaat gttttacggt tatgagaagt cgtttcagtc gcttaagcaa
      241 ttggaacaag ccattataga ctatattgat tactacaaca ataaacgaat taagataaaa
      301 ctaaaaggac ttagccttgt gcaatacaga actaaatcct tcggataaat tatttgtcta
      361 actttttggg gtcagtacac ctaaaacttt gatgatatac gttttcttgt gagaatattt
      421 acttcatttt tgcctaaaat tcaatgttta ctcagtattt ggattatgaa aaatcgaggt
      481 ctaaatctag atacattttt tctgaagaca aatcattttg accaccgagc aagagatttt
      541 caaaaaaagc tgttaaaaac tcagaacgtc gctgtaaaat ctttgcatta tctaatacca
      601 aggcatcacg aaaatatttg gaatgttgct gaaatggtgt attatcaata tcaaaaccaa
      661 actcacgaag atactgaatc aaaaagaccg ttactgtccg agtgtttcct tcgcgaaatg
      721 gatgaatctg ccagattcct gaaataaaat gctggatttg tttaaccaca tccgcctgag
      781 ttagtgtcgc atatgcaact tgtttttcct gattaaaatc ataatctaag gtcatttgaa
      841 tcatggagta atcagagtac acaacacttt caccattcaa aacaggttca ttctttgtga
      901 tattggtctg acgaaattgc cccaccggaa tagagggttc aaatatatct tgaaacaact
      961 ccttatgaat agcaagtaag gtcgcaggac taaagctaaa gcctcttcga gacaatagtt
     1021 ctacaatacg tagagaaacc aagtctgcct cttccgtact tgcatcaata gtatggtgat
     1081 aagccgttgc atcctcataa acctgctcat aagtcagttc tccccgggac tgtttctcag
     1141 ccaaagattc catatacgct gatggcacta gattgtcaac tttctgcaga ccaaaaccta
     1201 tccgccataa atcacgcttc gcttcataag acaagtttgg attgtcaatg ttgtaagttg
     1261 gttgcataga aatatccttt caattgtttt tcttcattac cagtaaatca cgaaattggt
     1321 tggaaactgg cttaaactat aaaaagtcct cacaataggt tctatgatat atatatatac
     1381 ttcatgtaaa taataaaaga gttataatca gcactactga taaattcaat accgatttcg
     1441 ccatcttaaa acttagtcaa acgaataccg ttgatttcca gttcattaag tcgttgacct
     1501 gtcaagactt ctcgttcctt caactggaaa agttaagggg gaactaatac aaatcctcta
     1561 cgcttctata ttcaatgatt tcatttttga taatattctt tttaaactca aaatgtttta
     1621 aaggattatc gattaacact aactttggaa tatcatctag atttgtgacc aaggataaaa
     1681 taaaatccga ttgaaactcc ttgacttttt caaattcttt tgcagttaaa cgcacacgcc
     1741 ccttagattt tcggatacct tttacttcgg caaggtaaga atactcttgg acatctacct
     1801 gaaagtcata tccatcacca taaagacggg catcggttaa ctgtccacct tggaattttt
     1861 cttctttatc aaaatgaagg ataaaataat tctcagcctc cattcccgtt tcctgtagac
     1921 gtttgaattt agttcgaact attggttttt caacgatggt tatgcctgtt ttcttccctt
     1981 cactggcaag taacatttta actatttcag cataactatg aacatcttca tttccaaaca
     2041 tcatatcaat taaatcttta cgaaaacgat aaacttcagc tttctgccac caaccttttc
     2101 gattattgtc aaaataagga tcaaataaat ccattctatt ttttacaact ccagttgttt
     2161 cagcaattcc cagagaaacc acatagcgat aaaactccga cttgctagag cattgaaatt
     2221 cttttataaa aagcttgtca aacttggcaa gaccgtagcc aattaaattt aacaattcat
     2281 aatgagggtg tttggacatc actttctcca tcaatagata ttgatttcat tataccaaaa
     2341 aacacggctc atcaccgtgt ttctgtgttt atttcaccaa cttcttcatc tcatcaatac
     2401 gtgctacggt cacatcatac ttggcttggt agtcggcttg tttgtctttt tctttttgga
     2461 caacttctgg tttagcgttg gcgacgaagc gttcgttgct gagttttttg ccaaccatgt
     2521 cgagttcttt ctgccatttg gcaagttctt tttccagacg tgccaattct tcttcgacat
     2581 tcaagaggtc taccagtggc aagaagattt cggctcctgt aataacgctt gacatagcca
     2641 gttcaggtgc agggatggtt gatgcgattt ccaagtgttc tggatttgta aagcgtttga
     2701 tgtagttgac attgctgtta aagaaggctt ccaagtcact atcgctagtc ttaacaagga
     2761 tggtgatagg tttgcttggt gctacattta cttccgcacg cgcattacga acggcacgga
     2821 tcaagtcttt gagactttcc acaccagtgt gggcagcaag gtcctcaaag gctgggttga
     2881 cagttgggta tgctgctgtc acgatagagc cttcagcgta ctgaccaaag atttcttctg
     2941 tcacgaacgg cattattgag tgaaggagac gaaggatttt atccagcgta tagaggagaa
     3001 cagatcgagt aattacctta tcgtcttcat tgtcgctgta aagaacttcc ttggtcaact
     3061 caacatacca gttggcaaat tcttcccaaa tgaagttgta aaggatatga ccagccacac
     3121 caaactcgaa cttatcaaag ttttcagtaa cttttgcaat ggtttcgttg agattgtgga
     3181 gaatccagcg gtccgtcaca ttaccagcct cacctgttgc aacttttgtg acattgtcat
     3241 gcgccacatc cagcgtcaaa cctccattgt tcatgaggat atagcgagaa atgttccaaa
     3301 tcttgttaat aaagttccat gaagcatcca ttttctcata agagaaacgc acgtcttgtc
     3361 ctggcgcaga accgtttgaa aggaaccatc gaagggcatc ggcaccgtat ttctcgataa
     3421 catccattgg gtcaatcccg ttaccgagag acttagacat cttgcgtcct tgctcgtcac
     3481 gaatgagacc gtggataagg acgttttgga atggttgacg gcctgtgaat tccaatgact
     3541 ggaagatcat acgagacacc cagaagaaga tgatgtcgta acctgttacc aaggttgaag
     3601 ttgggaaata acgtttaaag tcttctgagt cgacttcagg ccagcccatg gttgaaaatg
     3661 gccagagggc agaactgaac caagtatcca agacgtcttc gtcctgagtc catccgtcac
     3721 cttctggagc ttcttcgccg acatacattt caccatcagc attgtaccag gcagggattt
     3781 ggtgacccca ccagagctga cgagagataa cccagtcgtg gacattttcc atccattgaa
     3841 ggaaggtatc gttgaaacga ggtgggtaga attcgacctt gtcctctgtg tcttggttgg
     3901 caatggcgtt cttagccaat tggtccatct tgacgaacca ttgagtagac aagcgaggtt
     3961 caaccacaac acctgtacgc tctgagtgac caacactgtg gacacgtttt tcgattttga
     4021 cgagggcacc gatttcttcc aacttagcaa cgactgcctt acgagcttca aaacggtcca
     4081 tgcctgaaaa ttcaaaggcc aagtcattca tggttccgtc gtcgttcatg acgttgactt
     4141 gtggcaagtt atgacgttgg ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt
     4201 tcacgacacc agtaccaaac tcaggatctg cgtgctcatc tccaacgatt gggatgagtt
     4261 tattagcgat tggaaggatg acatttttac caatcaagtc cttgtagcgc gggtcttctg
     4321 ggttgaccgc aaccgcaacg tccccaaaca tggtctcagg acgagttgta gcaacttcaa
     4381 ggacgcgtga accatcttcc agcatgtaat tcatgtggta gaaggcacct tccacatcct
     4441 tgtgaatcac ctcaatatca gaaagggctg tgcgagctgc tgggtcccag ttgatgataa
     4501 actcaccacg gtagatccag cctttcttgt aaaggtccac aaagacctta cgaacagctt
     4561 ttgacaaacc ttcgtcaaga gtgaaacgct cacgagaata gtctacagag agccccatct
     4621 tgccccattg ttccttgata gtagtggcat attcgtcttt ccattcccag actttcgtca
     4681 agaaagactc acgaccaagg tcatagcggg aaatgccctc accacgcaag cgctcctcta
     4741 ccttagcctg agtggcaatc cctgcgtggt ccatcccagg aagccaaagg gtatcaaaac
     4801 cttgcatgcg tttttgacgg atgataatat cttgcaaagt tgtatcccaa gcgtgaccaa
     4861 ggtgaagttt acctgtaacg tttggtggtg gaataacgat tgaataaggc ttagcctttt
     4921 gatcgcctga aggcttgaaa acatcagcat caagccattt ttggtaacga ccagcctcaa
     4981 cctcggctgg attgtattta gatgaaagtt ctttagacat gtgtgtgtcc tttctctatt
     5041 ttgtttattt tattttgaat ctgcttagca gcttcttctg cagacaaatt cgtattattt
     5101 attttaaagt agtggtgcaa ctcattcggt tgatattggg aatttaattg aagtgtttca
     5161 gcggtctcta aaatttctct ttcagatacc tcaatatgtc gttttaaggg tttgtgcttt
     5221 aatcgattct ccgttcgatt tcgacgtatg cactcttcaa gacttgtttc caactcaaca
     5281 aaaagaatct cttgatgata atcatccaac aaattctgaa tttgctttaa atatatcaga
     5341 tgatactgat ttgaaaaatc aattacatct gttaaaatta ctgatcgctg atttcttgca
     5401 cttgctccaa ggaatgaaaa agtgattcca cgaacaaatt cccacatttc ctctgtatag
     5461 tcctgataga tttctagtgc aaaatcaatg gcttgatggt tataaaatag ggtagcatcc
     5521 gtcagtcgag ataattcttg accaatggtc atttttcctg atgctggagc accaatgatg
     5581 aaaagatgca tcaaatcacc tcccactcgc tcttcagcaa gccatatatc agactgtcac
     5641 agcggtttcc ttgaacatcc tttcggtctc ggatgcgagc ttcgagggta aagccaagct
     5701 tttccgcgac tcgttgactt tgaaggttat atccaaagca agttagttca atcttgtgaa
     5761 gacccaaatc tttaaaggct aagtcaatca aggcacgcgc agcttctggc acataacctc
     5821 gcccccaata gtctgggggt aaggtatagc cgatttccag cacatcatct tcatggcgat
     5881 ggttgaaatc gacagagcca gcgatcttat ccgttccttt gacgacaatc ccgtagcctg
     5941 ctgggagatt ttccttttga ttacgctccg gaagaatgtg ctccagataa taaatctcat
     6001 cttccaaggt cttgactgga ggaaaacctg ctggataggc gacctctggc aaactagcgt
     6061 aggcatggat atcctcagca tccgccacag tacggactcg taaaacgaga cgttctgttt
     6121 cgattttatc tgacagctca gttcttgcca tccttcttcc tcgctttttt gatgaaactg
     6181 cccttcatat ctacacgctt gtccagatag cgataaacgc gctgatatcc atctcccatg
     6241 aaataggttg gggcaaacag ttgattttta aaatgtccct tttcatccag gaattctggg
     6301 gcaacaagtc gctcaagaat cttggcaaag atgtggcaaa taccgtcttc ctcaacaatc
     6361 ctatctaccc gacaatctaa aacaagtgga caggcgtcta aaatagaaat ctgagttcgt
     6421 tcagaaattt cataatgcac tcccaaacgt tccaatttct cctgatgact gataaaacca
     6481 gcctgctcca tcgcaagcat agaagtttca tcagaaatat tcacagtaaa tttttgatac
     6541 tgtttgatct gctctgcggc attctctctc gcaacgactc caatcacaac ccaatctcct
     6601 agactataag aggaactaca ggtcgtgatg ttatagccaa aattctaatc ttgatatcct
     6661 aaaataaaaa caggaaaacc ataatatagt ttacttgtgt taaaagattg cttcataaca
     6721 accccctttg actaagacgt aaaagaaaag ccctgccatc tacatgacag ggacgaatgt
     6781 gtttatccgc ggtaccaccc aatttcgggc agttgcccgc aactctcgtc tttaaagcaa
     6841 aaagacactt ttatttcaat ttttcatcca ttagcaacca gttttgacac atttgtggac
     6901 ttctcagcgc cgccactttc tgtaaaatgt gggattcaaa acacctctaa tggctctatt
     6961 gtagcaagat tttttcggaa atgcaaggct caagaaaaat tacttgaact tgatgccatt
     7021 ttctttcaat ttcttgatgg tagctgggcc gattcctttc aaggcaagga gttctttttc
     7081 agtccagttt ttgaaatctg aagcagactt gattccttca tcatagaaag tcttggcacg
     7141 atcaagtgga agtccaccca aatttgctgc aaaatcttct acagaattag ctactttttg
     7201 agcttgctct ttggtagctt ctactgcttg ttcaactttt ttagaaacag ccttaccagc
     7261 tgcgcttgct gactgttctg cacgcttcac gacttttttg tctcatctac aaccttagtc
     7321 acagtacttg aaaaggcacc tgcgcgacgg aggccatttc gtaattgttt tttacgattg
     7381 agtttctttg acatgataaa atcctttcaa taaaaaatcc ctgctctgac aaggatttaa
     7441 cataaacatt ctatcaagat tttagtgaaa aatcaagaat ctttctcgtt taataatttc
     7501 gctcaccaaa caagccttct ggcaaatcat ggaatccctt gcctactttg aagttttcaa
     7561 acttaccaag caagaactga taagcatcca agcgactctc gtagcgaatc atggcatctt
     7621 tggcacgctc gtcctggtct tcttcgtaaa ctttttggct ttccttgtgc gccatgtcgt
     7681 taatcatgat ttgggtattg acccaagctt caaattcctt cataaattct tgttcgtaac
     7741 tcattttttc tccttattct aatccacgga aatagtccgt atcaatctca cggtagggct
     7801 ggatatcctg aacgtattcc agtacacctt ggaattctcc gttttcatcg tgtactgcag
     7861 cataggtgat gtggacaaac tttcctcgcg actcagactt gaaccacatt tcatacttgt
     7921 ccttggtccc ctcacgaagt cccttcatga tagccttgac cttgtccaaa tacttaggcg
     7981 gatgacagag ttcgacattg cgcccgactt gggacggcgt ccgtttgaaa atcatctcat
     8041 cagctggcgt attgtcattg taatactgga aaatatcttc tttattgaca aaggtaatct
     8101 ccatagggag atgattgagg atgagattgg cctgctcgac tgaaagatag ccattaccaa
     8161 aagcctgttg actatggcgg tccagcacag cttccttttc cttaggggta aaggtaatgg
     8221 taaaatggcc ttctggcgta tctatgactt gttgaacttg accttctgcc gtatctagct
     8281 gtacaggctc ctctgcaatc ttttcctcaa taaagatctg tcgttctggc acccatttct
     8341 ctgacggacg gatgatggca tagccatagg catcactctc ctccgcaatc tgaagccagt
     8401 catcctgagt aaaagactca aggagaatca tgagcaggat ggactcttcc ttgaaaatca
     8461 tactttcaaa ctctgtcgca aaagcttcaa aagcttcctt tacactgcta attgacactt
     8521 ctggtagtga cttggctgtc gttagagctg tttgaaagag ttccctaatc tgatcatcca
     8581 ctccccacat aactttggga ggtgaatcat gtccatagcg ctccatgata ggaaagaaga
     8641 gttcttcctt acgttggtaa tggatgtcaa attgacccac aagtcccatc tgacgcacca
     8701 aacccttacg catctccgcc agcatttcct cgtcttccat agactcatag gtatctaaca
     8761 atctacgaat gcgaatcaag gccgcacgga gagccagatt ttcttctttg aagacacgaa
     8821 ctgggtgacc tggatgctca gtatctgaaa cttcgacacc tttgatagca tttttaaaaa
     8881 gattggcatg gacatcacag agttccataa catcttcaaa agtgacgccc gaatccgagt
     8941 tcatcagctc gtgctccata agggaaatct cgatggctga cacgcccgta aaggtcgcat
     9001 caaagcgatc ttgaaccgac tcaggagagg cgccattgtg caattctaac aaaatatccc
     9061 gtaggacatg aatccgttca tctgtcatta gtctaatcca atcacttcgt agccattcgc
     9121 ttccagtgtg cgtacaatct tgtccatagg agttcctgct agcttagaac cctgtttaag
     9181 tgatacttta cgaccaactg tattgcgcat taagggattg gcaaggggtt taaaacccaa
     9241 ctccactaga atttccaaga cttctggatg cttgtccacc acttctgcaa caggaattga
     9301 cacatcgatg atattgtcca tgacgacctc cattgtatgt tctaatactc aatgaaaatc
     9361 aaagagcaaa ctaggaagct agccgcaggt tgcttaaaac agtgttttga ggttgtggat
     9421 agaactgacg aagtcagtaa ccatacctac agcaaggtga agctgacgtg gtttgaagag
     9481 attttcgaag agtataaaat gattttactg cttatattat accatatgga gagagattaa
     9541 aaaactagca gtggggttcc tgctagtttt ctgcgacttg gattaccatc tagataagtt
     9601 taaagaacca atccaatatc ttgtaaaaca gcaattgaac tgcaaaagaa atgatcatcc
     9661 aaaagaattt cacaatccca ataatcggtt tgataaaaag aatagcaaga agaccaaaaa
     9721 aatttcattc tctctcctct ggcttaatga gccaccgagc cgtatccatt agcttatctg
     9781 caataaaaag tttgcccaga ccaacaacga catggtcagc tttctttatc gttttcatca
     9841 cttttacacc ttgcacggtc aaaaagtaat taccataaat tttagctaga gtttttatga
     9901 gtcccatatt actctccttc gatgatttca gcctcttttc ggattgtttc aacatcttct
     9961 tgatattgaa cttcactata gttgactagc ttggataatt ctttctgcaa gctttccccc
    10021 attggtttca tagttgcaaa ggttaaacca cctgaaatga taccacccaa gataggaata
    10081 aatttcccca ttcctttggc cagccctccc ttggtaagat tcacaccaaa tattcttaag
    10141 acttttttca aaataggata ccaaagcgtc tttgttaaag ctttattagg tactgttttc
    10201 attacctgtt tggcaattgt tataccacca gcacgtagca aagcagcagt tccattcacc
    10261 cccaacatga cgcctagata aagcaaaagg gtattttgag catcttcact caactcatcg
    10321 cgtgaagccc aaagatcctc ataaccataa atataaccta attcttgggc caatttcaga
    10381 gagaaaccat aaaattgagc cacatcagct ggaatggtaa ttgccatagc aagccctcca
    10441 ggtaatcctg ccaaaacaga agtcccactc gctaataaaa cattatccct aatacaggca
    10501 ttagccactc gatctaagat ttcttgagat aagagagacg ttggaccttg ttccaataag
    10561 gtaggaatgt cctgtggacc caattctttg gaaaacttat ccactaaaaa tttcgaacga
    10621 tcaaccttaa caacaggtag tttcaccact tgttgcaata cttgcatagc taagtcttgt
    10681 tcagccatat acaaactcct tatttctttt ttgcacctta tcatattata tttcctacaa
    10741 aaaaagcatt ttctaattag ataacattaa aaaatacaga accccttttt tatagtgaaa
    10801 tgaaac
//
LOCUS       DAVGJJ010000070        10634 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000070 DAVGJJ010000000
VERSION     DAVGJJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.070"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            69..566
                     /locus_tag="VVE35_001567"
     CDS             69..566
                     /locus_tag="VVE35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU3061730.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            566..2221
                     /gene="dnaX"
                     /locus_tag="VVE35_001568"
     CDS             566..2221
                     /gene="dnaX"
                     /locus_tag="VVE35_001568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU3061731.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFKMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2249..2443
                     /locus_tag="VVE35_001569"
     CDS             2249..2443
                     /locus_tag="VVE35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU3061732.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2593..3363
                     /gene="sufC"
                     /locus_tag="VVE35_001570"
     CDS             2593..3363
                     /gene="sufC"
                     /locus_tag="VVE35_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU3061733.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3391..4653
                     /gene="sufD"
                     /locus_tag="VVE35_001571"
     CDS             3391..4653
                     /gene="sufD"
                     /locus_tag="VVE35_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU3061734.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4664..5890
                     /locus_tag="VVE35_001572"
     CDS             4664..5890
                     /locus_tag="VVE35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU3061735.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5877..6317
                     /locus_tag="VVE35_001573"
     CDS             5877..6317
                     /locus_tag="VVE35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU3061736.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6371..7783
                     /gene="sufB"
                     /locus_tag="VVE35_001574"
     CDS             6371..7783
                     /gene="sufB"
                     /locus_tag="VVE35_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU3061737.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     VKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(7982..9223)
                     /locus_tag="VVE35_001575"
     CDS             complement(7982..9223)
                     /locus_tag="VVE35_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU3061738.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9326..10435
                     /locus_tag="VVE35_001576"
     CDS             9326..10435
                     /locus_tag="VVE35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU3061739.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3147 a         1948 c         2321 g         3218 t
ORIGIN      
        1 aaaatccttt gtttactaaa caagggattt ttcttttgtc tctgctcctt ttttgatata
       61 atagttctat gttaaaatca gaaaaacaat cacgttatca aatgttaaat gaagaattgt
      121 ccttcctatt ggaaggcgaa accaatgttt tggctaatct ttccaacgcc agtgctctca
      181 taaaatcacg ttttcctaat accgtatttg caggctttta tttgttcgat ggaaaggaat
      241 tggttttagg tcccttccaa ggaggtgttt cctgcatccg tattgcacta ggcaagggtg
      301 tttgtggtga ggcagctcac tttcaggaaa ctgttattgt tggagatgtg acgacctatc
      361 tcaactatat ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg atgatgaaga
      421 atggtcagtt acttggagtt ctggatctgg attcttcaga gattgaggat tacgatgcta
      481 tggatcgaga ttatttggaa caatttgtcg ctattttgct tgaaaagaca gcatgggact
      541 ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat atagaagtca
      601 aaacttctcc cagttagttg gtcaagaagt tgtggctaag actcttaaac aagcggtgga
      661 gcaagagaaa ataagtcacg cttatctttt ttctggtcct cgtggaacgg gaaaaaccag
      721 tgttgctaaa atctttgcca aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg
      781 caataactgc tatatttgtc aagcagtgac ggacggtagt ttagaagatg tcattgaaat
      841 ggatgcagct tctaataatg gggtagatga aattcgcgaa attcgtgata aatctaccta
      901 tgcgcctagc cttgctcgtt ataaggttta tatcatagat gaggttcaca tgctgtctac
      961 aggggctttt aatgccctcc taaagacgct ggaagaacca acacagaatg tagtctttat
     1021 tttggccact actgaattgc acaagattcc tgctactatt ctatcccgtg tgcaacgttt
     1081 tgagtttaaa tcaattaaga cacaggatat taaggaacat attcactata tcttagaaaa
     1141 agaaaatatc agttctgaac cagaggctgt ggaaatcatt gccagacggg cggaaggtgg
     1201 aatgcgggac gccttgtcta ttttggatca agccctgagt ttgacacagg gaaatgagct
     1261 gacgactgct atctctgaag aaattactgg caccattagc ctatcagcct tggatgatta
     1321 tgtggcggcc ttgtctcaac aggatgttcc caaagctttg tcttgcttga atcttctttt
     1381 tgacaatggt aagagcatga ctcgttttgt gaccgatctt ttgcactatt taagagactt
     1441 gttaattgtt caaacagggg gagaaaatac tcatcatagt tcagtctttg tagaaaattt
     1501 ggcacttcct caaaaaaatc tgtttaaaat gattcgctta gcaacagtga gtttagcaga
     1561 tattaagtct agtttgcagc ccaagattta tgctgaaatg atgaccgtcc gtttggcgga
     1621 aatcaagccc gaaccagctc tatcaggagc ggttgaaaat gaaattgcta cgctgagaca
     1681 ggaagttgcc cgtctcaaac aagagctttc taatgcaggt gcggttccta aacaagttgc
     1741 accagctcct agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt
     1801 gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa atctaattcg
     1861 tttgcagaat gcctggggag aggtaattga aagtctaggt gggccggaca aggctctgct
     1921 agttggttct caaccggttg ctgccaatga acaccatgct attcttgctt ttgagtctaa
     1981 cttcaatgct ggtcaaacta tgaaacgaga caatctcaat accatgtttg gtaatatcct
     2041 cagtcaggcg gcaggttttt cacctgagat tttagctatt tccatggagg aatggaaaga
     2101 agttcgcgca gccttttcag ccaaagccaa atcttctcaa actgaaaaag aagtagaaga
     2161 aagcctgatt ccagaaggat ttgaattttt ggctgataaa gtgaaggtag aggaagacta
     2221 aagaaagatt tcatgataca ataagtttat gaataaacaa caatttatta ttatggcgct
     2281 atttacagct gctgagacct attttttcaa tgaagcctgg atgactggcc gctatattat
     2341 ggcagccttt tgggcaattt tactctttag aaatttccga gtcagttatg tgatgggtaa
     2401 aatcgttgat gtcatcgatc agcattttaa taggaaagac tagccctcag cttccagaca
     2461 aaatcaaagc cttttaggct tttttttgtt atactagaaa agtatattta tagaattttt
     2521 gctctatttc tggggaaatc agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag
     2581 aaaggaacta tcatgtcagt aatagagatc aaagatcttc acgttgagat tgaaggaaaa
     2641 gaaattttaa aaggggttaa cctgaccctg aaaacaggag aaattgccgc tatcatggga
     2701 ccaaatggta caggtaaatc gactctttct gccgctatca tgggaaatcc aaactatgaa
     2761 gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga agtggatgag
     2821 cgtgcgcgta tgggactttt ccttgctatg caatacccat cagaaatccc tggaattacc
     2881 aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag aagatgatga gaagatttca
     2941 gttcgtgagt ttattactaa gctagatgaa aaaatggaat tgctcaacat gaaagaagaa
     3001 atggcagagc gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt
     3061 cttcaacttt tgatgttgga gccaacattt gctcttttgg acgagattga ctcaggtctt
     3121 gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt
     3181 ggtgctatga tcatcactca ctaccaacgt cttttgaact atatcacacc tgatgtggta
     3241 cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg
     3301 gaacgtgaag gatacgcaaa attagctgaa gaacttggct acgactacaa ggaagaattg
     3361 taattccctc gtatctttta ggagaagtaa atgactagag aaaatattaa acttttttca
     3421 gaaatgcacg ctgaaccaag ctggttggct gatctccgtc aaaaagcttt tgacaagatt
     3481 gagactttgg aattaccagt tattgagcgt gtcaaattcc accgttggaa tctgggtgat
     3541 ggaacgatta cagaaaatga gccatcagca aatgttccag atttcacagc tttagatcat
     3601 cacttgaagt tggtgcaagt aggaactcaa actgttttcg aacaaactcc agttgagtta
     3661 gctgaacagg gtgttgtctt cacagacttt cactcagctt tagaagaaat tccagagctg
     3721 atcgaagaat tcttcatgtc atctgttaag tatgatgatg acaagttggc ggcttaccac
     3781 acagcttact ttaacagtgg tgctgtactc tatattccag ataacgtaga aattacagag
     3841 ccaattgaag gaattttcta ccaagatagc gatagcaatg tgccgtttaa caagcatatt
     3901 atgattatcg ctggtaaaaa ttctaagatt agttatctgg agcgtttaga gtcacgcggt
     3961 gaaggaagtg acaaagcaac tgccaatatc acagtggaag tgattgcacg ttctggtgcg
     4021 caagtcaagt ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta cattagccgt
     4081 cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga
     4141 aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag
     4201 gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat
     4261 ggctgcaact caatcggaaa cattctacaa catggggtta tccttgaaaa agcaactttg
     4321 actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc gcaacaagag
     4381 agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt
     4441 gatgaaaatg acgtaactgc aggacatgca gcctctattg gtcaggtaga tccagaagac
     4501 atgtattacc tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt
     4561 ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt
     4621 gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc
     4681 gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct
     4741 ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctatta acagctacta
     4801 tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc
     4861 ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga
     4921 agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga
     4981 aattctcgct gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat
     5041 tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga
     5101 cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc
     5161 cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt
     5221 agcccaccaa gttggggcaa ttatggtagt ggatggtgct caatctacac ctcatatgaa
     5281 gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg
     5341 tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt
     5401 agaatttggc ggcgagatga ttgattttgt ctacgagcaa tttgctagtt ggaaggaatt
     5461 gccttggaaa tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc
     5521 agttgattat ctggaaaaga ttggtatgga tgccgttgaa gctcatgaac aggaattgat
     5581 tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga
     5641 tttggctcaa cgttcgggtg ttattgcctt taacctaggt gatctccatc ctcacgatct
     5701 tgcgacggct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc
     5761 cttgcttcag tatttggaag tcccagcaac agctcgtgca agtttttata tctacaatac
     5821 caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg
     5881 cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac
     5941 atcaccaagg gaagttagaa gatgctgagc aaatcagtct caacaatccg acttgtgggg
     6001 atgtcatcaa cctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc
     6061 taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag
     6121 gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc
     6181 aaaaagatga gcgtcaaggc caacttggag acgcggcatt cttgtcaggt gttgccaaat
     6241 tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa
     6301 atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag
     6361 aaaggatact atggctgaag aaagagtaga accaaaacca attgaccttg gtgaatataa
     6421 atttggtttc catgacgatg tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg
     6481 tgttattcgt gaattatctg ctgttaaggg tgagcctgag tggatgttgg agttccgttt
     6541 gaagtcttat gaaaccttca aaaaaatgcc catgcaaact tggggagcag acttgtcaga
     6601 gattgacttt gatgacttaa tctactacca aaaaccatct gacaaaccag cccgttcttg
     6661 ggatgatgta cctgaaaaga ttaaagaaac ctttgaacgt atcgggattc cagaagctga
     6721 acgtgcttat ttagcagggg cttctgccca gtacgagtca gaagtggttt accacaacat
     6781 gaaggaagag ttccaaaaat taggtattat ctttacagat acagattccg cactcaagga
     6841 atacccagac ttatttaaac aatactttgc gaagttggta ccgccgacag ataacaagtt
     6901 ggcagccctc aactcagcag tatggtcggg tggaactttt atctacgtgc caaaaggtgt
     6961 caaggtagat attccacttc aaacttattt ccgtatcaat aacgaaaata taggtcagtt
     7021 cgaacgtacc ttgattatcg ttgatgaggg agcaagcgtc cactacgtag aaggatgtac
     7081 agcaccaaca tattcaagca atagcttgca cgctgccatt gtagaaattt ttgctttgga
     7141 cggagcttat atgcgttata ccactatcca aaactggtct gataacgtct ataacttggt
     7201 aacaaagcgt gctaaggctc aaaaggatgc cactgttgag tggattgatg gaaacttggg
     7261 tgccaaaacg actatgaaat atccatctgt ttaccttgat ggagaaggag cgcgtggtac
     7321 catgctctct atcgcctttg ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat
     7381 tcacaatgct ccacatacca gctcgtctat tgtgtctaaa tccatcgcta aaggtggagg
     7441 aaaggttgac taccgtggac aagtcacctt taacaagaac tctaagaaat ctgtttccca
     7501 cattgaatgt gataccatta tcatggatga cttgtcagca tcagatacta ttccatttaa
     7561 tgaaattcac aactcgcaag tggctttgga acacgaagcc aaagtatcta agatttcaga
     7621 agagcaattg tattatctca tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat
     7681 tgtcatggga tttgtagaac cctttacaaa agaacttcca atggaatacg cagttgagct
     7741 gaaccgcttg attagctatg aaatggaggg atcagttgga taaaatttga ttttatactc
     7801 ttcgaaaatc tcttcaaacc acgtcagcat cgccttaccg tatgtatggt tactgacttc
     7861 gtcagtttca tctacaacct caaaacagtg ttttgagcaa cctgcggcta gcttcctagt
     7921 ttgttctttg attttgaaga tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt
     7981 tttataattt ctcgttaaca aaacggacaa actgattcca ccaaactttt aagaagaagg
     8041 ctttttcaat tttcttgtct gctaccattt cgaaactagg gcgctctgtg gtgatgtaac
     8101 cttgaccaat caagtccttg tcttcataag tcaaatggcc aaccactgtt ccagcttcaa
     8161 gcggtgctgg gattgctttg gaatcaggtg tgaattgaac agattgggaa gattgattcc
     8221 caacacgttc gattagatag atatcctctg gagccactgc agttactgta tcttcttttc
     8281 catcttgtac aggggctttg ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa
     8341 gtgtaaatgt agaagaaata taatccatta gggaagatgt agctgtaaat cgagcgtaag
     8401 gattattgtc ttgatgatct gcatttaaaa caactgtgat gactctcatg cctttttcga
     8461 cagtagtacc aacaaaagac tctccagcct tatctgttgt tcctgttttt agcccatcaa
     8521 aaccaccacg gtaagcaggc ataccttcta acatgtagtt ggttgaagtg attgtcattc
     8581 cagcaaaagt agaagaaggt tttttggtga tttccaagac ttgtgggtat tttttgatga
     8641 ggttgcgagc aacaatagcg acatcataag cactaagctt attttcctca tcttttttag
     8701 aacctgggta aatgttatcc cctagagttt cattgttaag acctgtcgta ttgacaacag
     8761 tggcatcctg aattccccat tccaagagtt ttgcccgcat catatcgacg aaatcttttt
     8821 ctgagccagc aattttctca gctagggcaa tagcggcgct gttggcacta gataccagag
     8881 ttgcttcaag caactcttcg acagtataat tacgggcctc cataggaata ttactggctt
     8941 cagaatttgt cgtcaattga taaggataat cagaaatatc tacaggagtg gagagggtaa
     9001 tacttccgtt ttccaaagct tcatagacca gataaacagt aatcaatttt gttatggaag
     9061 caatttcgac aggttgcgtt gcatccttct catagagaat tttaccagta tttgcctcaa
     9121 cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc aacagcagta gaagcacccc
     9181 ctaaaagaga gacagttaac aaagttaaaa atattttttt catagtagtc ttattctatc
     9241 ataaagaaaa aaaatattct tgctttaata attcatctgt taagcttttt gaaaatatgg
     9301 taaaataaag tgagggaggt aactcatgtt tcgtagaaat aaattatttt tttggaccac
     9361 agaaatttta ctcttaacca tcatctttta cctatggaga cagatggggt ctttgattaa
     9421 cccttttgtt agcgtgctta atacaattat gattccattt ttattagggg gctttcttta
     9481 ttatttgaca aaccctattg ttactttctt aaataaagtc tgtaaactca atcgtttgct
     9541 tggtatttta attaccttgt gtactttggt ctggggaatg gtcataggtg ttgtctatct
     9601 cttacctatt ttgattaatc agttatctag tttgattata tctagtcaaa ctatttatag
     9661 tcgagtacaa gacttaatca tagacttatc taattatcct gcgctccaga atttggatgt
     9721 agaagctaca attcagcagt taaacttatc ctatgttgat attcttcaaa atatcctaaa
     9781 tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt atcagtactg ttttgatttt
     9841 gattatgact ccagtttttt tggtttattt cttattagat ggacataaat tcttgcccat
     9901 gcttgaaaga acgattctaa agagggatcg cttgcatatt gcaggcttat taaagaattt
     9961 aaatgcgacg attgctcgct atattagtgg agtttcgatt gacgcaatca ttataggttg
    10021 tttggcttat attggctata gtattattgg tttaaaatat gctttagttt ttgccatttt
    10081 ttctggtgta gccaatttaa ttccttatgt ggggccaagt attggtttga ttcctatgat
    10141 catcgcaaat atattcactg atccccatag actgctgatt gcagtgattt atatgcttgt
    10201 tgttcagcag gtagatggca atatcttata tcctcgaatc gtaggaagtg ttatgaaggt
    10261 tcatccaatc acgattttag ttttactttt gttgtcaagc aatatctatg gtgtagttgg
    10321 aatgattgtc gcagtgccaa cctattctat cttgaaagaa atttctaagt tcttatcccg
    10381 tttgtatgaa aatcataaaa taatgaaaga acgagaaaga gaattagcta agtaaaagtc
    10441 aggagaaccc tgatttttct ttactggaag tggcctttag attagaagac tgaaaataag
    10501 ttaaagtctt aaactaattt tcacagctaa gaatagtaga agttaatctg ataaaaatcg
    10561 aaaaaaccag tggaattctg tgtcagggta agttccactg gttttcatag tctattaaag
    10621 ttcgaatgaa acct
//
LOCUS       DAVGJJ010000071        10529 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000071 DAVGJJ010000000
VERSION     DAVGJJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.071"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(56..1480)
                     /locus_tag="VVE35_001577"
     CDS             complement(56..1480)
                     /locus_tag="VVE35_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU3061740.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVAEKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2025..4304)
                     /gene="pulA"
                     /locus_tag="VVE35_001578"
     CDS             complement(2025..4304)
                     /gene="pulA"
                     /locus_tag="VVE35_001578"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU3061741.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4416..6374)
                     /gene="ligA"
                     /locus_tag="VVE35_001579"
     CDS             complement(4416..6374)
                     /gene="ligA"
                     /locus_tag="VVE35_001579"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU3061742.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6467..7645)
                     /locus_tag="VVE35_001580"
     CDS             complement(6467..7645)
                     /locus_tag="VVE35_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU3061743.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLQSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7725..8589)
                     /locus_tag="VVE35_001581"
                     /pseudo
     CDS             complement(7725..8589)
                     /locus_tag="VVE35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
     gene            complement(8592..10493)
                     /locus_tag="VVE35_001582"
     CDS             complement(8592..10493)
                     /locus_tag="VVE35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU3061744.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3162 a         2332 c         1891 g         3144 t
ORIGIN      
        1 tattatttta tagcaaaaaa ataaaaccag gaaaacaatt tcctgatttt acacgttatt
       61 tcacatcaaa aacaatggat ttgacatttg tcatcgcttc aatgctatat ttaattcctt
      121 gcactccagc tccagaacct ttgacaccaa ggaatgggaa attatctgga ccacgctggg
      181 ttttattatt aatgtggact gtacctactt caagtttttc agcaatttca aaggcttttt
      241 tgaaatcatt tgtaaagact gatgattgaa ggccgaattc agattcgttg gcaaaggcaa
      301 tagcttcctc tacactagcc acacgaatga ttggtaaaac aggaccaaat ggctcttccc
      361 atgccacttt catatctttt gtaacttggt caaaaagcac tggccagagg agattgccct
      421 cacgtttgat tggtgtaaga gcctgagcct ctttttcttg tgcatcctca atcaagcccc
      481 aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat atcagcattg tcaaatggat
      541 caccaactgt taatttagaa acttcttcct gaagcaaagt agctaatttc tctgctacac
      601 tttcgagaac aatgacacgt ttaatggccg tgcaacgttg tcctgagtag ctaaaggctc
      661 ccgcaacaat ttgcttggca gcatgttcca aatctgcatc ttctagtaca agagctgcat
      721 ctttcccacc aagttccaac atgataggac gcataccagc taaacgacca atacgttctc
      781 caataggagt tgaacctgta aagttgatga agttgacttc tttgtgctca atgatataat
      841 ccccaatttc tgaaccacga cctgtaatgg tgttgaaaac acctgccgga atccctgctt
      901 cttcaaatgc tttagccaac aagagtccag aaatggaacc ttgtgttggt ggcttaaaca
      961 tgaccacatt ccctgcaatc aaggcaggtg caattttaga agcagataaa ttaactggat
     1021 aattaaaggg agcaatcgct agcacgatac caactggttc acgacggaca acagccagtt
     1081 tgtttttact tgttgcctca aaaccaccac cttccattgc ttgtccagtg atacggagac
     1141 cttcctcagc agcataacga atcaagtctg ctgtacgcac tacttctcca attgctgctt
     1201 taatcccttt tgctacttct ttggcaagga tagtaccaat ttcttcctta tcgcgttcta
     1261 aaatagctgc tgttttatgc aaataagccg cacgttcaac tgctgataaa gctcgccatg
     1321 ctggcagggc tgcacgcgca gcttgcatag cctcatcagc ttcagtctga gtcatggctg
     1381 gaactgtacc caattcttct tgattgattg gtgaataaat cgtaatttct tgttcagatg
     1441 atttccattt tccatttact aaattctgat atcttgtcaa attcttgtcc tcctgaaaat
     1501 gattaagata tattttacca aattttcaga aaatcacaac cctttttgca aaatttttga
     1561 gattattttc acaaacttga tttttcaaag tatactcaat aaaaattaaa aaaatccact
     1621 acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat
     1681 ctatagtaaa ataaaaaaac tgaacaattt ggttggggac agccaaacca atttctcaca
     1741 atgtttcaga aacaagggtg tgctattcca atttcagcct actataactg tcatagattg
     1801 ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca agtgttcaaa
     1861 aactttgata ggaggtcttg ttttgtgaaa atatttatca aattttctat acaagtgagc
     1921 tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta ctaatactca
     1981 taactgggaa tttgtctgtg taaaaatagc gagatagatg gtatttataa aacactcaag
     2041 acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac
     2101 atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaaccacc
     2161 aagaggtgtt ctttgccgtg aacttcatag ataaggtagc cgctatgttc aatcgcagaa
     2221 tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt
     2281 gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag
     2341 ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta
     2401 tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct
     2461 tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg
     2521 accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga
     2581 agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt
     2641 cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg
     2701 acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg
     2761 tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa
     2821 ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcgatt
     2881 tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca
     2941 aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac
     3001 ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag
     3061 gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca
     3121 acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga
     3181 taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt
     3241 gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca
     3301 tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg
     3361 tagcctagct tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca
     3421 gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat
     3481 ttagtcaaat cacgaatatg catttcacag ataactgcct tacatggatt ttccaagcgc
     3541 caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc
     3601 agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt
     3661 gtttggtgat gagggaattg gacttgatac tgataagtct tacctaccaa atcttcttca
     3721 acatccaaac tccagacacc gattgtattg tccttatgat tataagagta gctattgcct
     3781 cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca
     3841 acttgcactt ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca
     3901 cggcaaccca attctccttg gtaaccccaa tgatgatcaa aactagcact gttaatggcc
     3961 ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca
     4021 gagtaataaa tcctatcatc gccttccaaa atccagacct ctgttaatag gggatagtga
     4081 ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt
     4141 tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg
     4201 taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattct
     4261 ccattcttac gatggtaatg aatgcgcata gggtagttat acatttttta tttttccttt
     4321 ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata
     4381 gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca
     4441 tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct
     4501 accacgacga ggtcggtctt tttagaaaca ctgcctgtca ctttggcacc cagactttcg
     4561 agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc
     4621 aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag attgacccca
     4681 gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga
     4741 cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga atctgcctga
     4801 gacagatttt caattgaatg gaaatattga agtaaaagct gactagcctt gcttccgaca
     4861 tgacgaattc ccaaaccaaa taagagcttc tcggcagaat tttcctttga tgcttggata
     4921 gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct
     4981 tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca
     5041 acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag
     5101 ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca
     5161 aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca
     5221 gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat
     5281 acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg
     5341 gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt
     5401 acaacaccgg tacggccaac tgtccagtca actgataaga gttgagcttc tttttcttca
     5461 gcagggaact tgtaggctac tgcccacttt ggagccttaa cggtaaaacc aagttcttct
     5521 tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg tagattttcc
     5581 cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt
     5641 cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta
     5701 tcacgagttg aagggctggc ttcttgatag agaaacgttg caagattacg cttggcaact
     5761 actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca
     5821 ggctctccat tttcttggcg cacttggtta acttggtcaa aggaagcgcg tggcatgtaa
     5881 cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca
     5941 cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca
     6001 accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa
     6061 atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca
     6121 cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca
     6181 tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa
     6241 gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgaaaccgag
     6301 ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc
     6361 attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa acgagaaggg
     6421 cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt
     6481 caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa
     6541 ttatcaggaa agaagagaga tgtacacttt gcaagactgt cataaatcct tttgcaatag
     6601 gcataaatag aatagctaag gtaaaaattg tactcagtac tctcccaaga aattcgctct
     6661 caaccttggt ttgtacttga gtaaaaaagt gaatattaaa aatcgtcata aacaattcac
     6721 aaactaaatt tccagaaaag gaaagaaaag ttggaagtgg taatcccatc ataaaaactc
     6781 cgacacctgt caaagccagt aaaatcaaaa gattataaat attagcttta attttactag
     6841 ctagaagagc cccaatgatg gaaccaatag cccccatagt taaaatactt gcataggctc
     6901 cttctgaccc gtaaagctga ttcgaaaagg gaagtagaaa ttcaaaagct gcaaaaaaga
     6961 aattaacgct ggaagctacc agcaaaagga agaaaatttc ttgctgatgc cagatatagt
     7021 gtaacccatc cttgatatct acaaaaatat ctctcccagt aaaagccttt ttctcttgaa
     7081 cttttgcttc ctcttttgga aggaaagcca ctagaacaaa agcaatgaaa aaagtcagcg
     7141 agtctagcag tagcgtcata tggagacttg caaactgtaa aacaaggaag gaaagaacag
     7201 gagagctaac acctacaacc tgcaaaacca gctctaagcg agaattatag atcacaatct
     7261 catctttctc caccacttca gttatgatag ctttattggc tgtgcgagaa aaggcaaaag
     7321 caatagcctg cacaatgtta gcaacaatca aagcgccaat catccagcta tcattcctta
     7381 tgaaagaaat agccagacaa agaatcccac aaacaagatc tgccgtcatt aaaatcttac
     7441 gacgagaaaa acggtctgaa ataactccgc caaagggatt gacgagaata gacgtgacga
     7501 gctcagaaat ctgatacatt cctaaaactg tctgtcctat agtccccata gaagccaacc
     7561 agacactatt tccataatca tagagcatat ttcccatttt attgatagcc ccacggctaa
     7621 tcaactgcac tgcatagcga ttcatattaa agctcctctc aaattttgaa actattgtat
     7681 caaaaccgaa aggagctttt tattttttcc cttatttggg aaaattaact tttgacaaat
     7741 ttttcgtagt gttcctgata ataggctact tgctctggaa gacctaacac atcaaaaata
     7801 tgcatggcct cttgcatctg cttacagcct tctttacact gtcctttttg atataaggca
     7861 aaacctttta aataatggaa aacattacgc tcataaagct taataccttt gtcaataatc
     7921 ttctctgtat aagcctcaaa atagttggca ttataaaaag aagaatactc taaacaatgc
     7981 tggtaacaat tgagggccaa aatcaaaact aatctcttat ggcgactaat ctcttggtaa
     8041 aattcctccc tctccataac ttctctacca atccgagtga catagtctac atcgtagaaa
     8101 ctatagaggt taccgaaaag aatcaactca tacatggtcc attcttctgt tttgaagaga
     8161 taatctgcta ccttatccaa atcatcctgc ttcatatcat aagtcgcatc tctttgacaa
     8221 atcagacctt gtagcaaaat ccagttcagc tcaaaataaa gggaagtcgt cgaactctta
     8281 gacttttcaa gttgttctct ttgaagcttt tgaaaacctg caatatcgtt tgaatagtaa
     8341 agtgggataa tctgtgccat catagacaca atgttcatga ttatgaaaat tccttgcctt
     8401 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8461 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8521 tgctgcttcc tttaaagaat aatttccact tgttcgaaat ttacgaaata cttttccaag
     8581 atgttccatc tttacacctg atctgataat tcttcccact caagcatagc ttcttcctga
     8641 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8701 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8761 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8821 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8881 ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt aatctccaag gtagagagtt
     8941 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9001 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9061 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9121 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9181 ttaacatcat ctcctgagaa aaggaaggcc ccaagacggt tgcggatttc aacttctggt
     9241 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9301 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9361 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9421 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caaaacttcc
     9481 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9541 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9601 ttttctagtt gtttacggcg agattgagca cgtttggtcg ttgaagcacg aactagattg
     9661 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9721 tcagtagcta gcttttgctc cttcaactca acaaaacgag agtaattccc cacatagcga
     9781 tccaaggaat gcttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9841 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggag
     9901 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
     9961 aggagcattt tagctagagc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10021 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10081 taggtaaagc cacctgcttg gcgaaaattt tcagataagc ggtcataatc tgacatcagt
    10141 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgttta
    10201 tctgtccgac gtaagtcatc aaagacatga agcatctcat catagatggt attttcagac
    10261 tcaaaacggc tatcttgagc taggtaagac agagaaatat cttttttctt attgatttct
    10321 ccactagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10381 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10441 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat catacaagta
    10501 gtatagcatg tttccctaag gcattcaag
//
LOCUS       DAVGJJ010000072        10528 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000072 DAVGJJ010000000
VERSION     DAVGJJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.072"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(6..1220)
                     /locus_tag="VVE35_001583"
     CDS             complement(6..1220)
                     /locus_tag="VVE35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU3061745.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKEFLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1669)
                     /locus_tag="VVE35_001584"
     CDS             complement(1376..1669)
                     /locus_tag="VVE35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061746.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIYQLESLVPLLYPGFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1831..2283
                     /locus_tag="VVE35_001585"
     CDS             1831..2283
                     /locus_tag="VVE35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU3061747.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2318..3706)
                     /locus_tag="VVE35_001586"
     CDS             complement(2318..3706)
                     /locus_tag="VVE35_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3061748.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3772..4734
                     /locus_tag="VVE35_001587"
     CDS             3772..4734
                     /locus_tag="VVE35_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU3061749.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQDLKDYMEG"
     gene            complement(4731..5102)
                     /locus_tag="VVE35_001588"
     CDS             complement(4731..5102)
                     /locus_tag="VVE35_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061750.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHCFEVADRTDEVSSKHVFEVADRTDEVSSKHVFEVVDRTDEVSSKHVFEVVDRTDE
                     VSNHTYGRATLTWFAEIFEEY"
     gene            complement(5166..6176)
                     /gene="ccpA"
                     /locus_tag="VVE35_001589"
     CDS             complement(5166..6176)
                     /gene="ccpA"
                     /locus_tag="VVE35_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU3061751.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKDVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6601..7200)
                     /locus_tag="VVE35_001590"
     CDS             complement(6601..7200)
                     /locus_tag="VVE35_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061752.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7202..8299)
                     /locus_tag="VVE35_001591"
     CDS             complement(7202..8299)
                     /locus_tag="VVE35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3061753.1"
                     /translation="MLERLKSIHYMFWTSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8299..9036)
                     /locus_tag="VVE35_001592"
     CDS             complement(8299..9036)
                     /locus_tag="VVE35_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061754.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYFVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(9038..9922)
                     /locus_tag="VVE35_001593"
     CDS             complement(9038..9922)
                     /locus_tag="VVE35_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061755.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9909..10103)
                     /locus_tag="VVE35_001594"
     CDS             complement(9909..10103)
                     /locus_tag="VVE35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061756.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMLTDWFDQALLRQFIRGALGFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(10100..10306)
                     /locus_tag="VVE35_001595"
     CDS             complement(10100..10306)
                     /locus_tag="VVE35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061757.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3183 a         2415 c         1919 g         3011 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagccg
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtggccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagaattcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacgattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attcaacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgcgaaag gcgaggttaa gaacgtagag atagcaggta ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca
     1441 tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa
     1501 ataagattag atggtagagt acatgaaaga ctaaaaatcc aataaaacct ggataaagaa
     1561 gcggtaccag actctctaat tgatagatca tgatcacttc taccaagact gaaactagga
     1621 taattccata atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcccttcct
     1681 tcacttcttt cctgctatcc tttcttttat tttatcatat atttatatag attttaagcc
     1741 ctaatattct tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat
     1801 tatagtaaga aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc
     1861 gcaatcgatg gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta
     1921 cgaaacgacg ctaaactcac catcgcacat gtcattgaca cacgcgctct ccaaagcgta
     1981 tccacctttg atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa
     2041 gagtacgaaa aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg
     2101 ggaaatccaa agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc
     2161 ctcgttggcg caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa
     2221 tacatactcc gccatgctaa ggtcgatttg ctggttgtga gagaacaaga aaaaacctta
     2281 taatcacaaa gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat
     2341 ggcgttcgta agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat
     2401 atttttcttc ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct
     2461 tgccatctgg aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg
     2521 ggtccacccc catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat
     2581 ccacttgccc tatcaaatct tgctgagcag gtgtcttctt ggctacttta tctgctgcct
     2641 tatcaagggc ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg
     2701 ctgctcgaac aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct
     2761 ctaaatccca agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct
     2821 tgaccttatt gaaatgatag tcacggctga caaagacttt ttctgaagac aaaacaccaa
     2881 aatgttccaa ggccttgtgg atcccatctt gttgattgct agtggtaatg tgcttggcag
     2941 cttccttgac actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca
     3001 tttcaaggtc attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt
     3061 ctttcccaac tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg
     3121 caaaaggatt gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt
     3181 cttctggtgt catcagcatc aaaacttggt agataggctg attcatcagg tgaagcaggt
     3241 cctcttcctt ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc
     3301 gagttaaaac agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg
     3361 acatgatttt agaacccaac atggcatcct tggtccctag agcaatctcc gtgccctctt
     3421 ttttagcata gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga
     3481 ctctgtcttt actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat
     3541 cgtccatcaa ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc
     3601 cttgttcttt gacaatctta atcgcatcct tagtggattt caaaacactc ttgcgattgt
     3661 tgaccaaggt tccatcgata tcaaaaaaaa cagctttgac ttccatccta tcccaatctc
     3721 cccttttgtg atacaatgat tataccacat ttcagaaaga gtgagtaaat catgcctaag
     3781 aaaatccttg ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct
     3841 gttgtgacga gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa
     3901 gtccacgcct tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg
     3961 gtcctctacc agaaaattaa agaggaagca gataactacg atggagtggt gatcacacac
     4021 ggaaccgata ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg
     4081 cctatcgttc taacaggagc catgcgtagc tccaatgaac tcggtagtga tggtgtttat
     4141 aattacctaa gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg
     4201 gtcgttatga acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat
     4261 gtcagcacct tccagactcc aacacatggc cccctcggtc tcatcatgaa acaggaaatc
     4321 ctctacttca aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta
     4381 gtccctatca tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta
     4441 gaacacttgg acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg
     4501 gctcaaaaat tagaaagcct tctgcaaaaa ggaatcccag tcgctctggt atcacgatgc
     4561 tttaacggta ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa
     4621 gcaggcgttt tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc
     4681 gccctcaatg ccggactaac aggacaggat ttgaaagact atatggaagg ctaatactct
     4741 tcgaaaatct ctgcaaacca cgtcagagtc gccctaccgt atgtatggtt actgacttcg
     4801 tcagttctat ctacaacctc aaaaacatgt tttgagctga cttcgtcagt tctatctaca
     4861 acctcaaaaa catgttttga gctgacttcg tcagttctat ctgcaacctc aaaaacatgt
     4921 tttgagctga cttcgtcagt tctatctgca acctcaaagc agtgctttga gctgacttcg
     4981 tcagttctat ctgcaacctc aaagcagtgc tttgagctga cttcgtcagt tctatctaca
     5041 acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc tatggtattc
     5101 attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg attccctgat
     5161 tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg taagagaact
     5221 tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat actaatggca
     5281 ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa gcgtgagatt
     5341 tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacatc cttatcagcc
     5401 aaaccgttca agactcctgc tgctaactca tcacctgtca caactgctgc agttgcattt
     5461 gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt agattcaaat
     5521 accaaaccct cactataagc gattcctgct tttttcaagg tttccttgta gccaactaaa
     5581 cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat acgctcattt
     5641 tctttagcaa ggtaactcac tgcatcaatt gttgcttgct tatagtcaat attgacactt
     5701 ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg tgaacgcgaa
     5761 aattctgagc gaattttatc tgtcaagtga taacccatat agataatgcc atctacctgc
     5821 tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc gctattagct
     5881 aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc caaactcgaa
     5941 aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt tttacttgca
     6001 agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc tagcactttt
     6061 ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac cgtcgccatg
     6121 gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc attcattcct
     6181 tttcctgtcc tttctatctc acacattctt ttacaagtag aggtgctgat tgaagctcta
     6241 tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac ttattccatt
     6301 ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa aaatttcata
     6361 gaaaacctag gtttagctcc ttgctaccac cttagactaa acaaaaagga ggaaactaag
     6421 ccctcctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag gacagtcaaa
     6481 tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatag
     6541 gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa tattatatca
     6601 tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac cagcatccag
     6661 ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt aaagctgatc
     6721 tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa tctcttgttc
     6781 tgtcaacgga ttgggacggg tcatcaccac ttccatcaat tcaggcgaat actccttgcg
     6841 tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt cctttaatac
     6901 ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct tgaaggtcgt
     6961 caccacaacc acctttgttt caagcctttc tgatcgtatc cactccaaga cttcaagacc
     7021 tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct tttctaatag
     7081 ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg gttgaagcgt
     7141 gagcaattga cacatggcat ctcgcaacat actttgatct tctgcgacta atactttcat
     7201 cttagtttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
     7261 ttacacttac ttctcctgaa aatggaaaaa cacgatctcg gactgtatgg agctcatctc
     7321 cttttagaaa agcaaagccg cagccatcat cacttactgt taaaatgagt tccttctctg
     7381 tccgttctaa ttttaagtaa gctttagacg ctttggcatg tttgatgata ttggtcacca
     7441 actcaagcaa aatcatagaa gccattgact ccaattcttg agtaaggcta gcagtatcta
     7501 gttggttatc cgtttccacc tcaattccag caatttctaa catctttttc acagtctcta
     7561 gttcggatgt caaagttcta gacttaagat tttccacaat ggttcgaact tcacgcattg
     7621 attctttgct aatttgctgt atttctctta attccttttc cacttgtgga taagcctgca
     7681 tttgaaatag ctgcaaggct agatctgtct tgacactaag catagcaaag gtatgtccca
     7741 gactatcatg caaatcctga ccgatacgat tgcgctcatt ttctgctagc aatagattta
     7801 tctgggtatt ttgcttgacc tgagcttctt tcaaatcctc gacaaggcga attcgaaccg
     7861 agcccaaagt catcaaatcg acaaaagtaa gaattacaag catatagact agaaactcaa
     7921 cttcgattct ctgaaaaatc aaaagtcgcc ccacaacaag gacttgagca aggagaaatg
     7981 tccatacatg taaagacttt aaactacgta cgccaaaatg ataaattaag agattggaaa
     8041 ggaaaaagaa aaaccagata taattcccag caacaaaggc ggtattccca gctacataga
     8101 ccagcatgat cccccagaaa agccaagata ggcgctggtt cttagttatt aaaacaccca
     8161 aatacgtcac tacaaataga atatcaacca ataaatgcca agcaggaagc tccccaatca
     8221 ctacaggtag gatgggaaaa agcataaaaa tcaaactggt ccaaaacata tagtgtatgc
     8281 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tagtcaaacc
     8341 aaataaaact gctgaaaaaa caagtaaata aactgtggct gatagattga tgccaccctc
     8401 gtttaagaag gtcttgagca actccatcaa ttgataggtc gggagacact taccaactgc
     8461 ttgcatccag tctggaaata aagaaatggg catccagagt ccgcctaaaa cagccaatcc
     8521 tagataaaga agattgccca caacagacat cagctgacta gttggtaaga gagtcaaggt
     8581 caagccaagt gctacaaaag caatactccc cacaatcagc aaaatcgcag ctccaaccca
     8641 ttttactaga ggcatgtcta cacctcttac aaaatgtcca actgtaaaaa caaccagaat
     8701 tgagacaaaa taatcaacca gcatacttgt tatctttgat agataatatt ctaccatatt
     8761 tacagggcta tgacgcaatg ttttctgcca gttgttcatc ttatcggtat gtaaaacagc
     8821 tggaaatgag aggatagttg tcgacatcat ggaaaaggca gtcatggaga tgaggtaatc
     8881 acgcataaaa ttcgcgggtt cacctggtgt gtcctgatag ataccagaaa aaaataaata
     8941 gaaagccgtc ggcatcccta ccgacaatag ataatagatt aattgtcgtt tggtcaataa
     9001 aaattctatc ttactaagtg ctatccatcg tttcatctta gttatctccc ttctgcgtct
     9061 cttcaaagat tgtattcaac aaactacgat tattaacttc gatttcttgc atcctacatc
     9121 ctgcttgggc taatagttcc cagaaagcat ctgcttctcg agtgactact tgtaaagaat
     9181 cttgctttag ggtccagttt tcaaccaaat ttgactgctc tacgacttcc ttgtaagcta
     9241 taggaaggat aaagtgcttt tctatttcct cgctacgcat ggctagaggc gtcgtatctc
     9301 gaatcaactc tcccttattt aagagcaaaa tgcggtctgc agtgtgttcc acttcctcaa
     9361 tataatggga cgaatagaga atggtgactc cttgcgcttt taactcctgg acaatttccc
     9421 aaaaacgttg acgagttgag gtatccatgg acgcagtagg ctcatctaaa aaaacaatct
     9481 ttggtcgccc aattaaggtc aagatgaaag aaaagagacg cttttgaccg cctgataatt
     9541 tttctgccaa ctgttctttt tgttgcttga caaactgcaa tagttgatca atttcctgat
     9601 tgctcaaagg atttggatag atacgttgaa agaaagcaat caactcttta acttttaatt
     9661 tctgaacgat tacattttct tgagggaggt aagccctagt atagtctaac ttagaactcg
     9721 tcactggtaa accttgaata gatacttgac cgcttgtgac cagtttatct ccaagcaaac
     9781 aatctaagag tgttgtcttt ccggcaccat ttggaccaat taaggcaaca cattcaccat
     9841 cgttgatttc aaaggagatg ttccgcaaaa tttccttgtc ttttattttc ttactcaatt
     9901 tttcaacttt aaccaccgtc atgagactct cctttcaacc actccattcc catagggaaa
     9961 acgacgaaaa tcataaatcc aaaccccaaa gcaccacgaa tgaattggcg aagcaaggct
    10021 tgatcaaacc aatcagtaag catttccact aaccatacca agagtgacag gccgataaag
    10081 aaatagatga tccctctctt cattcctcaa actccttttt cacatctctg actaatttca
    10141 aaccttctct aacaagccaa gacatgattc caaagccagc aaagaccttc caaggaaaat
    10201 ggtagaaatc ctcatccaat cccgaaaaca tgagataggt cataactcct gctactacta
    10261 aacttactgc gataatcatt ttatttctca tctcttcttc ccccatttca tactacaatt
    10321 atagtctttt aaaatcagag gaggcagaag cttctgtcac tagaaaatat gacaaatgtc
    10381 ataaaaaatt ctgttcaaaa caagcaagat acactataca ataaaacaca atcagaaaaa
    10441 tctaaggcaa cttcctcaaa agagatatca aactcaattc acaccataat gtaaactaat
    10501 acttatttaa aatcaaaaag agtagaaa
//
LOCUS       DAVGJJ010000073        10384 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000073 DAVGJJ010000000
VERSION     DAVGJJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.073"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(310..2568)
                     /gene="glgP"
                     /locus_tag="VVE35_001596"
     CDS             complement(310..2568)
                     /gene="glgP"
                     /locus_tag="VVE35_001596"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU3061758.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2594..4111)
                     /gene="malQ"
                     /locus_tag="VVE35_001597"
     CDS             complement(2594..4111)
                     /gene="malQ"
                     /locus_tag="VVE35_001597"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU3061759.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4661..5932
                     /locus_tag="VVE35_001598"
     CDS             4661..5932
                     /locus_tag="VVE35_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3061760.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6043..7335
                     /locus_tag="VVE35_001599"
     CDS             6043..7335
                     /locus_tag="VVE35_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3061761.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7337..8179
                     /locus_tag="VVE35_001600"
     CDS             7337..8179
                     /locus_tag="VVE35_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU3061762.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8825..9169)
                     /locus_tag="VVE35_001601"
                     /pseudo
     CDS             complement(8825..9169)
                     /locus_tag="VVE35_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
     gene            complement(9302..9712)
                     /locus_tag="VVE35_001602"
     CDS             complement(9302..9712)
                     /locus_tag="VVE35_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEU3061763.1"
                     /translation="MYELSNRDLDGIDIELGRYRTLANKIYLRRQELIHNKKHSTEDY
                     TGGKGKTVSSPTEATIIRIEEDQTLRYLEGFKLVVDTLMENLIESDLVIFKMRYLEAG
                     ATWEDVAEKLNKTTRYINSRRKVIAKRFVELKGY"
     gene            complement(10095..>10384)
                     /locus_tag="VVE35_001603"
     CDS             complement(10095..>10384)
                     /locus_tag="VVE35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061764.1"
                     /translation="FKETKRRAEEKKIYKRYQKKLRIILDAYDEIQSLKKDVEEIHKG
                     VVREITQKHSLAVYRTEVNPTSILPFLNPDISGHMNFSKEYHEIKEYLGKE"
BASE COUNT         3017 a         2199 c         1869 g         3299 t
ORIGIN      
        1 aatacaagag atatgaagta accaactacg aaaaaatata aggctgaaga cagtaaaaac
       61 cagcatttaa agccttcact gtatctagga tttccctatt tttagacaag attgacccaa
      121 atccacagca tttaaaaaag cgaatttcaa tcgaaactca cttttttaca aaatgtatta
      181 gtataaattt gtatcttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
      241 ggttgctcaa agcaccgctt tgaggttgca gataaagctg acgtggtttg aagagatttt
      301 cgaagagtat tagttcaagt gccagatgtc ttcattatac tgagcgattg tacggtcaga
      361 tgagaagaat cctgctttag aaatgttaac gatgacttta tccaaccatg cgtcacggtc
      421 ttcgtagtca gcaagcattt gctctttgac tttgatgtag tcttccaaat caagaagagt
      481 catgaaccag tctttgttga tcaattcatt gtaaagacgt tccaagcgct ctttgtttcc
      541 agctgcaaga actgcatcac taacgatgaa gtcaaccaat ggtttgatag cttcacgagc
      601 gtagaattcg cttgatttat atgctgcttt tgcgtaaaga tcgataacag tttctgaatc
      661 ttcaccaaag atgtagatgt tttcgtcacc aaccaactca gcgatttcca cgttagctcc
      721 gtccatagta ccaagagtca aagctccgtt caacatgaat ttcatgttac cagtacctga
      781 agcttcttta gaagcaagtg agatttgttc tgagatatca catgctggga taaggaaact
      841 tgctgcagta acgttgtagt tttcaaccat aactacttgc aagtgtggag ctactgctgg
      901 atcgttagca ataacttctg acatgcaaag gattaaatgg ataatgtctt gagcgattgt
      961 gtaggctgga gctgctttac caccaaagaa gattgtgatt ggacgagcag ggatgttacc
     1021 agctttgatg tcaaggtatt tgtggatcac gtacaaagcg ttcatttgtt ggcgtttgta
     1081 ctcgtgaaga cgtttgattt ggatatcaaa gatagaattt ggattgattt ccacaccttg
     1141 gtgttctttc aagtgacgag ccaatttacg tttgttgtga gccttgatgc tttccaattt
     1201 ttctttgaca gctgctttgt cttcataaga caaaagtttt tcaagctcat ctgcttcatg
     1261 gtgccaacca tctccaagaa tctcatccaa gtagtgagac aatcttgggt tagcatgcat
     1321 aagccaacga cggaaagtga taccgtttgt tttgttgttg aacttttctg ggtaaaggtc
     1381 gtagaaggct ttcaactcag aatttttcaa gatttcagta tggagtgctg caaccccgtt
     1441 aacactgtat ccgtagtgga tatccatgtg agccatgtga acacgtccgc tctcatcgat
     1501 gatttgaaca gctggatctt tgtactctgc cttcacacga cggtccaatt cttcgatgat
     1561 tggtaccaag tgaggaacca cttcttgcaa gaattcaaga ggccattttt caagcgcctc
     1621 agcaaggatt gtgtggttag tgtaggcagt catgctacga acaattgaga ttgcttcgtc
     1681 aagctcgata ccacgtgcag tcaaaagacg aatcaattca ggaatcacca ttgatgggtg
     1741 agtatcgttg atttggacaa ctgcgtagtc agcaaggtca tgcaagttgc ttcctttttc
     1801 gattgcttcg tcgatgatca attgcgcacc gtttgaaacc atgaagtatt gttggaagat
     1861 acggagcaat tcaccttgac ggtcactatc atctgggtaa aggaagagag ttaagttgcg
     1921 agcgatatct gtcttgtcaa agttgatacc atctttaata atagaagaat caactgaatc
     1981 caagtcaaac aaacgcaagc ggtttttagt cgctgtttca taaccagtaa catcaatatc
     2041 gtaaagagtt gatgtcaaag taaagtctgc aaatggtact tggtagctac ggcttgagcg
     2101 aaccaaccag ttttgctctg tcaaccatgc atttggaatt gtttcttgtt ggttgttttt
     2161 aagaacttgt tggaaaagac caaagtggta gttaagacca acaccgtcac cattcaaacc
     2221 aagagtagca attgagtcga taaagcaggc agccaaacgt cccaaaccac cattaccaag
     2281 agatggttcc aattcaactt cttcaacttc gatcaagtct ttacctgcag ctgcaagttc
     2341 ttttttaaca tcgtcgtaaa gaccaaggtt aatcaagttg tttgacaaga gtttaccaat
     2401 caagaactca gctgagatgt agtaaacttt tttcttacca gtgttgactg gtttttggct
     2461 gcttgcaagc ttgctgtagt taagaagagc aaggtaaagc tcttcattgc tacattctgc
     2521 aatggtttta ttgtaacgat tttgtacaaa ttcttgtagt gataacatgt ttagttgtct
     2581 cctgattatt gtcttatttc tttaaatcta ccaaattttc attaattcgg cgataaattg
     2641 ttgtcaagtc aagcaaacct tcctcgacag ctggtgtcaa ttgatcttca gtcatacgcc
     2701 aagaccagtt tccaccaagg gtagatggga agttcatacg agctgcctca tccaattcta
     2761 gtaaatcttg catagttgca attgccataa agctaactga tgaaaatact gtacgaagca
     2821 tagcgtgtac cactgtttcg tattctttac ggttcgtgta acgagccatg tactcacgag
     2881 tcgcatcatc aatctcatta cggtaccaac caagaaccgt attgttatcg tgtgttcctg
     2941 tgtacataac tgagttagca ggtgccaagt gtgggctatc aatgctttcg tcttctgggt
     3001 tgaaggcaaa ttgaagaatc ttcattcctg ggaagccagt acgttcacgc aattcgatca
     3061 cttcatctgt catgaagcca aggtcttctg cgatgatgtt tagctcacca agttcttcct
     3121 taacggctgc aaaaagcttg tagcctggac ctttcaccca ctcaccaggt gctgctgtat
     3181 cggaaccagc agggatttcc cagtaagatt cgaagccacg gaagtggtcg atacgaacga
     3241 tatcgtagat tttgaagctt tcacgcaagc gttcaatcca ccatttgtag ccgtctttgt
     3301 ccattgcttc ccagtcatag attggattac cccaaagctg accagttaca gaaaactcat
     3361 ctggtgggca tcctgcgata caagtagcct taccattgac atctgttttg aagagatgtg
     3421 gatttgccca catatcactt gaatcttccg ctacgtagat tggcatgtcc ccaacgattt
     3481 caatgtggtt gtcgttagcg taagctttca atttcaacca ttgttggaag aagaagtatt
     3541 gagtcacacg gtggtaaacc aacttgtctg ccaattgctc acgatagctt tcaagtgctg
     3601 aagctttacg agcacgagca tctgcatctg gccattcagt ccaagcaaga ttgtcaaaat
     3661 actctttgat agccatatac tcagcaaaga gctcaagcca tgattggttg tcttgagcaa
     3721 atttctcaaa atctttaaca tctccgactt caaagaaacg tttcaccgct ttttctaaaa
     3781 gaggacgacg tgcatagtag attttagcat agtcaacttc agacgcatcg ctaccaaagt
     3841 caactccttc aaggtcactt gcttccaaca aaccttgctc caccaagata tctaaatcga
     3901 taaaatgagt gtttcctgcg aaggctgaga aagattggta aggagaatcc ccgtaactag
     3961 ttgctcctaa tggaaggatt tgccagtaac gttgttttgt acggaccaag aaatcaacga
     4021 agtcgtaagc actttgacca aatgatccga ttccgtaagc tcctggaaga gaagagatgt
     4081 gcatcaacac accactttga cgttttttca taatagcacc tcgtgtgtta aaataatgga
     4141 acgttgcgta ttttgcagac gcaaacgttt gcgttactta taagtatact ccttttcaag
     4201 cataattgca agcgttttta aaaaactttt ttgattattt ttaaggagac taactcattt
     4261 tagcaaagat aacttaaaac tatctaatcc taaggtttga ctctcacgag acagtctaca
     4321 aagtacaaaa cctccttagg gagcttgaat ttatatgtaa caaagcacaa acgtccatag
     4381 aaaatagata gggattagaa ccagggaggt agcccctcct ggtttccctc tttagacaga
     4441 ttccatatat ttttattaaa aatactgtat tcttatctat ttcatacttt tcttctatta
     4501 aaatgcaaaa ttatgctatc aaatcaagct taaaaatttt ttaaaatttt tacaaaaaat
     4561 acttgcaacc gttttctatt tgtgctatac taagctcata aaggaaaacg tttgcgtttc
     4621 cttatcaata aaacttgcta ttctttggga ggaatacact atgtcatcta aatttatgaa
     4681 gagcactgcg gtgcttggaa ctgttacact tgctagcttg cttttggtag cttgcggaag
     4741 caaaactgct gataagcctg ctgattctgg ttcatctgaa gtcaaagaac tcactgtata
     4801 tgtagacgag ggatataaga gctatattga agaggttgct aaagcttatg aaaaagaagc
     4861 tggagtaaaa gtcactctta aaactggtga tgctctagga ggtcttgata aactttctct
     4921 tgacaaccaa tctggtaatg tccctgatgt tatgatggct ccatacgacc gtgtaggtag
     4981 ccttggttct gacggacaac tttcagaagt gaaattgagc gatggtgcta aaacagacga
     5041 cacaactaaa tctcttgtaa cagctgctaa tggtaaagtt tacggtgctc ctgccgttat
     5101 cgagtcactt gttatgtact acaacaaaga cttggtgaaa gatgctccaa aaacatttgc
     5161 tgacttggaa aaccttgcta aagatagcaa atacgcattc gctggtgaag atggtaaaac
     5221 tactgccttc ctagctgact ggacaaactt ctactataca tatggacttc ttgccggtaa
     5281 cggtgcttac gtctttggcc aaaacggtaa agacgctaaa gacatcggtc ttgcaaacga
     5341 cggttctatc gcaggtatca actacgctaa atcttggtac gaaaaatggc ctaaaggtat
     5401 gcaagataca gaaggtgctg gaaacttaat ccaaactcaa ttccaagaag gtaaaacagc
     5461 tgctatcatc gacggacctt ggaaagctca agcctttaaa gatgctaaag taaactacgg
     5521 agttgcaact atcccaactc ttccaaatgg aaaagaatat gctgcattcg gtggtggtaa
     5581 agcttgggtc attcctcaag ccgttaagaa ccttgaagct tctcaaaaat ttgtagactt
     5641 ccttgttgca actgaacaac aaaaagtatt atatgataag actaacgaaa tcccagctaa
     5701 tactgaggct cgttcatacg ctgaaggtaa aaacgatgag ttgacaacag ctgttatcaa
     5761 acagttcaag aacactcaac cactgccaaa catctctcaa atgtctgcag tttgggatcc
     5821 agcgaaaaat atgctctttg atgctgtaag tggtcaaaaa gatgctaaaa cagctgctaa
     5881 cgatgctgta acattgatca aagaaacaat caaacaaaaa tttggtgaat aaaaaatttg
     5941 ttcaaggggg gtggaaatca aatccccctt tgaatttatc aatagagaca caaataattt
     6001 agctttctta taaaaaagta gtatcctatg aaaggagtta atatggaaaa gcaacaacct
     6061 agtaaagcag ccctgctgtc tatcattcct gggttaggac agatttacaa taaacaaaaa
     6121 gccaaaggtt ttatcttcct tggtgtaacc atcgtatttg tcctttactt cctagcactt
     6181 gcaacccctg aattgagcaa cctcatcact cttggtgaca aaccaggtcg tgataattcc
     6241 ctctttatgc tgattcgtgg tgccttccat ctaatctttg taatcgttta tgtactcttt
     6301 tatttctcaa atatcaaaga tgcacatacg attgcaaaac gcattaacaa tggaattcca
     6361 gttccacgca cactcaaaga catgatcaaa gggatttatg aaaatggctt cccttacctc
     6421 ttgatcattc catcttatgt tgccatgacc ttcgcgatta tcttcccagt tatcgtaacc
     6481 ttgatgatcg cctttaccaa ctacgacttc caacacttgc caccaaacaa gttgttggac
     6541 tgggttggtt tgaccaactt tacaaacatt tggagcttga gtaccttccg ttctgccttt
     6601 ggttctgttc tttcttggac tatcatttgg gctttggcag cttctacttt acaaatcgta
     6661 attggtatct tcacagctat cattgccaac caaccattta tcaaaggaaa acgtatcttt
     6721 ggtgttattt tccttcttcc ttgggctgtc ccagccttca tcactatctt gacattctca
     6781 aacatgttta acgatagtgt cggtgctatc aacactcaag tattgccaat cttggctaaa
     6841 ttccttcctt tccttgatgg agctcttatt ccttggaaaa cagacccaac ttggactaag
     6901 attgccttga ttatgatgca aggttggctc ggattcccat acatctacgt tttgactttg
     6961 ggtatcttgc aatctattcc taacgacctt tacgaagcag cttatattga cggtgccaac
     7021 gcttggcaaa aattccgcaa catcactttc ccaatgattt tggctgttgc ggcacctact
     7081 ttgattagcc aatacacctt caactttaac aacttctcta tcatgtacct cttcaatggt
     7141 ggaggacctg gtagtgtcgg aggtggagct ggttcaaccg atatcttgat ctcatggatc
     7201 taccgtttga caacaggtac atctcctcaa tactcaatgg cggcagctgt taccttgatt
     7261 atctctatca ttgtcatctc aatctctatg atcgcattca agaaactaca cgcatttgat
     7321 atggaggacg tctaagatga ataactcaat taaactcaaa cgtagactga ctcaaagcct
     7381 tacttacctt tacctgattg gtctatcaat tgtaattatc tatccactgt tgattaccat
     7441 tatgtcagcc tttaaagcag gtaacgtctc agcctttaaa ctagatacta atatcgacct
     7501 caattttgat aactttaaag gcctcttcac tgaaaccttg tacggtactt ggtacctcaa
     7561 cactttgatt atcgccttaa ttaccatggc tgttcaaaca agtatcatcg tacttgctgg
     7621 ttatgcctac agccgttaca acttcttggc tcgtaaacaa agtttggtct tcttcttgat
     7681 catccaaatg gtgccaacta tggccgcttt gacagccttc ttcgttatgg cgcttatgtt
     7741 gaacgccctt aaccacaact ggttcctcat cttcctctac gttggtggtg gtatcccgat
     7801 gaatgcttgg ctcatgaaag gctacttcga tacagtgcca atgtctttag acgaatctgc
     7861 aaaactagac ggtgcaggac acttccgccg cttctggcaa attgttctac cacttgttcg
     7921 cccaatggtt gccgtacaag ctctctgggc cttcatggga cctttcgggg actacatcct
     7981 ctctagtttc ttgcttcgtg agaaagaata ctttactgtt gccgtaggtc tccaaacctt
     8041 cgttaacaat gcgaaaaact tgaagattgc ctacttctca gcaggtgcta tcctcatcgc
     8101 ccttccaatc tgtattctct tctttttcct acaaaagaac tttgtttcag gacttacaag
     8161 tggtggcgac aagggataat ttatccccgc cacccttttt cattttatac tcttcgaaaa
     8221 tctcttcaaa ccacgtcagc tttatctcca acctcaaagt tgtgctttga gcaacctgtg
     8281 gctagtttgc actttgattt tcattgatta ttagcaattg tcactgtaaa taatatcctt
     8341 gtagcaagca atttttctcc tagacttgaa ataaagcgca tttctctata atttgactta
     8401 ggcatcattt cacacctcaa tatagcgcct aaacccttga tattactgac tttttgaaaa
     8461 atctcatttg attagaaatc attaaaagtt cttaaaaatt ctcaaaaagt ccacaaaaaa
     8521 aagccccaca aaagggcaat aaatgacgag ttcagcaggc aagaaactag cacggtcaaa
     8581 cgtgcttttt taatacctgg taatattata gcatatcctc tcaaacttac atataaccag
     8641 aatcctgcaa aagaaaaaga taaaagcata gaggatagag cgaaaaccgt tcttactttt
     8701 attttgtcaa atagcaatat gagaagcaag gataaaaagc cctcagaaat aaatctaagg
     8761 acttaaataa cgacgtgttg gtttgctcac ttaattagtc tacttaatac tattcaatta
     8821 tagtttactt tgaaatgttc ttaatccttt ctgtaaattt ttgaatcgca ttcagttctg
     8881 caggtcttaa atgaggctac gctttagtcc tccccccatt tcgtttaata tgtccaaatt
     8941 caagtaaatg agtaagagag ccgtatttaa aactatatac gacccaaact ccatttctca
     9001 cccttttctt acgccatcct tttgaatatt tacctgttct tttagggcta gtttccctta
     9061 attctttaac tgtttcttta gcgacttgct ccgcaatttt atcaacttct tcattaacct
     9121 cttcgcacta ttctgctaaa atatttgcta attgtgctgc taaatccatt ttctaaaaac
     9181 tccttctatt ttttaaaata tcaacttttt accccctttt tgtctggagg tctccgactt
     9241 ggaaaaagct cccttcaccg gtaccctact ggccagaaag attttttaaa aggtggggga
     9301 gtcaatatcc tttcaattcc acaaatcttt tagcgattac ctttctacgg ctatttatat
     9361 aacgagtagt tttatttagt ttctctgcca cgtcttccca agtcgcacca gcttctaaat
     9421 atctcatttt aaaaattact agatcacttt caattaagtt ttccatcaag gtatctacaa
     9481 ctagtttgaa accttctaaa tatcttagtg tttggtcttc ttcaattcta atgattgtcg
     9541 cttcagtagg actagatact gtcttgcctt tcccaccagt ataatcttca gtgctatgtt
     9601 tcttattatg tatcagttcc tgtcttctca aataaatttt attagcaagc gttctatatc
     9661 gtcctaactc aatatctatc ccgtccaggt ctctgttact caactcgtac ataggcaagt
     9721 acctccactt aaatttaaaa atttttttat ctttcaattt gtcaaattgt aaattctgtc
     9781 aaactgacaa aaagcgctaa aagccttcca acactccact taccaggtat cattgtttta
     9841 agtttgacaa ctcttcagta tgacaagttc aagggaaatt tctttaattt atcccctcag
     9901 tttctcatat cttacattct gtgaaactca ctccattctg taaacccctg atatacctta
     9961 ctttcaagct attacttctt ttcagtttat gctttcttca ttttgtgaaa ctaatttact
    10021 gaaattaaaa acaaggccat ttttgatata gctatcaatt tcttgatatt caaatcagcc
    10081 ttgtttctga tgaattactc tttacctaga tattctttaa tctcatgata ttccttagaa
    10141 aaattcatat gcccgctgat atcaggattt aagaacggaa ggatacttgt tggatttact
    10201 tctgtccgat atactgcaag agaatgtttc tgagttattt ctctaactac tcctttatgt
    10261 atttcttcta catctttctt tagtgattga atttcatcgt aggcatctaa aattattcta
    10321 agtttcttct ggtaacgttt atatattttc ttttcttcag ctcttcgctt agtctcttta
    10381 aaga
//
LOCUS       DAVGJJ010000074         9860 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000074 DAVGJJ010000000
VERSION     DAVGJJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.074"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            267..362
                     /locus_tag="VVE35_001604"
                     /pseudo
     CDS             267..362
                     /locus_tag="VVE35_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(615..1235)
                     /locus_tag="VVE35_001605"
     CDS             complement(615..1235)
                     /locus_tag="VVE35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU3061765.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1235..1717)
                     /locus_tag="VVE35_001606"
     CDS             complement(1235..1717)
                     /locus_tag="VVE35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU3061766.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1717..2559)
                     /locus_tag="VVE35_001607"
     CDS             complement(1717..2559)
                     /locus_tag="VVE35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU3061767.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVDYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2654..3454)
                     /locus_tag="VVE35_001608"
     CDS             complement(2654..3454)
                     /locus_tag="VVE35_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU3061768.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3598..4977
                     /gene="glmU"
                     /locus_tag="VVE35_001609"
     CDS             3598..4977
                     /gene="glmU"
                     /locus_tag="VVE35_001609"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU3061769.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEITPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4987..5532
                     /locus_tag="VVE35_001610"
     CDS             4987..5532
                     /locus_tag="VVE35_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU3061770.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            5544..5858
                     /locus_tag="VVE35_001611"
     CDS             5544..5858
                     /locus_tag="VVE35_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU3061771.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5875..6567
                     /locus_tag="VVE35_001612"
     CDS             5875..6567
                     /locus_tag="VVE35_001612"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU3061772.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6644..7135
                     /gene="rocS"
                     /locus_tag="VVE35_001613"
     CDS             6644..7135
                     /gene="rocS"
                     /locus_tag="VVE35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU3061773.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7233..7823
                     /locus_tag="VVE35_001614"
     CDS             7233..7823
                     /locus_tag="VVE35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU3061774.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSAPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7832..9595
                     /locus_tag="VVE35_001615"
     CDS             7832..9595
                     /locus_tag="VVE35_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU3061775.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3047 a         1704 c         2095 g         3014 t
ORIGIN      
        1 ccaaaagtta gattttttct gtctaacttt tggggtgtag ttcatgtaca cctgatatga
       61 tgcgttttat aattttaaag actttttgac cagcctcatt tttttaactt gatactcagt
      121 gaaaagcaaa gattaaacta ggaagctagc tgtaggctgc tcaaagaaca gctttgaggt
      181 tgcagataaa acttgtgagg taaccaacat atataatgtg aagctgacgt ggtttgaata
      241 gattttagaa gggtatgagt ctggaagttt taatggataa tgcaagattc catagaatgg
      301 gtaagctaga gttcttatgt gaagagtttg ggcataaact tttacctttt cctccctact
      361 catcttagta tagaaaagtg aatctaaaat agtacataac tgcttctaaa acattcttat
      421 aaattgattt aaattctcaa atcatattat tcagttctta tttcattttg ctctacaatc
      481 ctgttgagaa gacacgtgtt catatcaaaa aggtattggc aagttgcaat acctttttac
      541 aaggttcttt tgtcttattt ttgtttcaac tgactatatc tcctatggtt ctagttcaga
      601 aggctaggct ataattatga ttgataagaa gtatcattcc aagtattgag agtgaatgtt
      661 tcaaaatcat gggtttctat aatggtcagg ctggcatttg ctagaccgcc atctttacga
      721 agaagtggtt ctttatagcc taggagagta cgaagactgg cagtaagatt ggcgccgtgt
      781 ccgacaatta gaatacgctc agctggacta tcttttaatg atttgataaa ttggatggtc
      841 cgctgagttg tactatagag ggattcggct ccgaacattc gagtgtcaaa ttgagcaaga
      901 tttgaacgaa aagcctggat ttgttgcggg taaatagctt ccagggttgc aattttcaaa
      961 ccttctaact tcccaagttg ccattcacgg agattaggaa cgatttctaa agaacagggg
     1021 gtatggagtt gactttggat aatctcagca gatttgaccg ctcgaggtaa atcacttgaa
     1081 taaatctgat caaaaggaat ttccttgaga tactggccaa gtcgttttag ggtttcaatg
     1141 gattcaggaa gaagaggaga atcaccgcta gcaccttgaa aacgaccttc ttggttccag
     1201 agggtacgac cgtggcggac aaagtagagt ttcattactt gatgtcctcc aaaatatcta
     1261 caaagtctgc ctttacaaag ctagccaagt cttgtggcgc gacgataatg ctgtgtccga
     1321 cttcgcctgc agagacaatc atttgatcca aatctagagc aattttatcg ataaaaatgg
     1381 gataattgtg tttctgacga attccgacag gattattggc tccatgaatg taaccagttg
     1441 ttttttctaa gtccttttgt ggaatcatgc tcactttttt attgccagaa attttagcta
     1501 gtttcttttc agacaagtgc tgagtgatag ggacaattcc gataatcggt ccggtcttgt
     1561 ctcccaaaag cgccaaggtt ttgaaaatct gatctcgttc ataaccttga ggaagctctc
     1621 cttctagggc atttatttga atcccctgat gagggatagc tgctttagat aggatttgtt
     1681 ccaccaatgt ttttttgatt ttaacttttt ttgccattat ttatatttat cctccaattg
     1741 actcatccaa ataccaagcc agattcccag cgcaaagaag aaggcgatga tgacataatc
     1801 gacaagtgaa agtcctgtgt attggatact ttcagcgttt cctgcatttg gaattaagat
     1861 caaaagggta cttgatagga cgatacctat gatgaaatga tagacgcgtg agtggtagtt
     1921 gtttaaggca taatccatca attttgaaaa aacgatgaga gttgcacctg caccaattcc
     1981 aatcggaaag aaggttccca agagatcaaa agttttaaaa ccagtcaaca taggagcata
     2041 gagtcccaaa atcaaaagta aattcgatgg gctgaggcca ggaaccaaga cgccaagggc
     2101 caatagtgcg cctgctagga cgaagttaag aaagctggcg cttaaggttc caacgacaaa
     2161 atttaaggca tatagtccta atccagaaat gataaaggtt gtccataacc aagctaaatc
     2221 aatcttgtct cggtcagatt ctcgagttga ttctttgagg aggctaggaa ctgtaccgat
     2281 aatagctccc gcaaagctcc ataatacaaa gacctgataa ttttcaagca ggtattcaat
     2341 cgggtaggaa aataagccga ttcccagaag cataccgatg gcaactggaa taaagtacaa
     2401 aacattttct ttaaggtctt taaagggatg ggccagaaag ccaatcattc gttcatagat
     2461 tcctaagatt gctgctagaa cccctccgga aattcccggt aggataaatc caagagcaat
     2521 tacaatccct ttaataacgc gtgctaacca tgagagcata tatttcctcc aactatttct
     2581 atttcaaaaa atccttacta tattgtatca caatcagaca caaaaagaaa aagcaaatga
     2641 taaacaaatg cttttaaggt tttaaaaaaa gctttcgaaa ggtttcttct ttatttttta
     2701 agggagagat aacgttgata tctaaatcgt ggtcaaagcc ggcaattttt cctttagatg
     2761 tgtattggtg aatatcataa tctaaatcag ttttaggact gctctccaaa aatcctgagt
     2821 ctgagccgta ggacggaatc caaacagagg taaacttgcc tgtatcaata ctgtgttctt
     2881 ccatgaagta gacaccaacg tagatgccga tgtttttagc acctagtgat gctagttttg
     2941 ctcgaaagtt ttcgacacct tcgttcatat tagacatggt tttgtcttcc acgtcaagcc
     3001 aatagtaact agggctgtaa ggagaggcag cattgtagaa aacttcggca gccttttcca
     3061 tttcttggac actttttcca gctacataag cgtagacagc aactgggaca ttccgctttt
     3121 gaagttcagt gatatgactc ttataggcct tgtctattcc attgataaat gaagcatcat
     3181 tttcttttgt cgtttgagca ccactgtgaa cacgaacaat agcacctgaa atattttgtg
     3241 agagggcatc gtagttgatt tcctcaggac gctgccagcc agagaggtca ataatcggtt
     3301 tgtctaagtg tttcaaagcc tgtgcttcaa tctgtgctat attggatttt gttttaaacg
     3361 attggttgtc attaagtggg cgattgatga ttaaaatgaa catcataatc cccaaaaaac
     3421 taaataaaat aagtggatga atttgttttc tcatatctta taattctacc ctaaaaatca
     3481 aaaaaaatca aaaaaatggg ttaaggaaga gactttagag cattttttca ttcaagagtg
     3541 cggaatgatt tgaaatatgg tataataaaa gggaatttct acagaaaaga gaagattatg
     3601 tcaaattttg ccattatttt agcagcgggt aaagggactc gcatgaaatc tgatttgcca
     3661 aaagttttgc acaaggttgc gggtatttct atgttggaac atgttttccg tagtgtggga
     3721 gctatccaac ctgaaaagac agtaacagtt gtaggacaca aggcagaatt ggttgaggag
     3781 gtcttggctg gacagacaga atttgtgact caatctgaac agttgggaac tggtcatgca
     3841 gttatgatga cagagcctat cttagaaggt ttgtcaggac acaccttggt cattgcagga
     3901 gatactcctt taatcactgg tgaaagcttg aaaaacttga ttgatttcca tatcaatcat
     3961 aaaaatgtgg ccactatctt gactgctgaa acggataatc cttttggcta tggacgaatt
     4021 gttcgtaatg acaatgctga ggttcttcgt attgttgagc agaaggatgc tacagatttt
     4081 gaaaagcaaa tcaaggaaat caacactgga acatacgtct ttgacaacga gcgtttgttt
     4141 gaggctttga aaaatatcaa taccaataac gctcaaggcg aatactatat tacagacgtc
     4201 attggtattt tccgtgaaac tggtgaaaaa gttggcgctt atactttgaa agattttgat
     4261 gaaagtcttg gggtaaatga ccgtgtggcg cttgcgacag ctgagtcagt tatgcgtcgt
     4321 cgcatcaatc ataaacacat ggtcaacggt gttagctttg tcaatccaga agcaacttat
     4381 atcgatattg atgttgagat tactccggaa gttcaaatcg aagccaatgt taccttgaaa
     4441 gggcaaacga aaattggtgc tgagactgtt ttgacaaacg gtacttatgt agtggacagc
     4501 actatcggag caggagcggt cattaccaat tctatgattg aggaaagtag tgttgcagac
     4561 ggtgtgacag tcggtcctta tgctcacatt cgtccaaatt caagtctggg tgcccaagtt
     4621 catattggta actttgttga ggtgaaagga tcttcaatcg gtgagaatac caaggctggt
     4681 catttgactt atatcggaaa ctgtgaagtg ggaagcaacg ttaatttcgg tgctggaact
     4741 attacagtca attatgacgg caaaaacaaa tacaagacag tcattggaga caatgtcttt
     4801 gttggttcaa attcaaccat tattgcacca gtagaacttg gtgacaattc cctcgttggt
     4861 gctggttcaa ctattactaa agacgtgcca gcagatgcta ttgctattgg tcgcggtcgt
     4921 cagatcaata aagacgaata tgcaacacgt cttcctcatc atcctaagaa ccagtaggag
     4981 cctatcatgg agtttgaaga aaaaacgctt agccgaaaag aaatctatca aggaccaata
     5041 tttaaactgg tccaagatca ggttgaatta ccagaaggca agggaactgc ccaacgggat
     5101 ttgattttcc acaatggggc tgtctgtgtt ttagcagtaa cggatgaaca aaaacttatc
     5161 ttggtcaagc agtaccgcaa agctatcgag gctgtctctt acgaaattcc agctggaaaa
     5221 ttggaagtag gagaaaacac agcccctgtg gcagctgccc ttcgtgaatt agaggaagaa
     5281 acagcctata cagggaaatt agaactcttg tacgattttt attcagctat tggcttttgt
     5341 aatgagaagt taaaactata tttagcaagc gatttgacaa aagtggaaaa tccgcgtccg
     5401 caggatgagg atgaaacctt ggaagtcctt gaagtgagct tagaagaagc gaaagaatta
     5461 atccaatcag gtcatatttg tgatgccaag acaattatgg ctgttcagta ttgggagttg
     5521 aagaaaaaat agaggaggtc agtatgggta aatctttatt aacggatgaa atgattgaaa
     5581 gagctaatag aggcgaaaaa atttcaggtc ctcctttgct agatgataat gaggagacta
     5641 agattttacc aacctcttct tcccgttttg gttatgccaa tcctaagaat catggtttta
     5701 gccaggaaac cttgaagatt caggtcgaac catctattca taaaagccgt cgcattgaaa
     5761 ataccaagag aaatgtcttc aattctaagt tgaataaaat cttattcgcg gtcatctttc
     5821 tcttgatttt gcttgtttta gcaatgaaac ttttgtaata gaaaaggaat tgaaatgaaa
     5881 ataggaatta ttgctgctat gccagaagaa ctggcttatc tggtccagca tttagataat
     5941 gcccaggagc aagttgtttt ggggaatacc tatcatacag gaaccattgc ttctcatgaa
     6001 gtcgttcttg tagaaagtgg aattggtaag gtcatgtctg ctatgagtgt ggcgattttg
     6061 gctgatcatt tccaggtgga tgcccttatt aatacgggtt cagctggggc agtagcagaa
     6121 ggtatcgctg ttggggatgt cgtgattgct gacaaattag cctatcatga cgtggatgtc
     6181 acagcttttg gctatgctta tggacaaatg gcgcaacaac cgctttattt cgaatcagac
     6241 aaaacctttg ttgctcaaat ccaaaagagt ttatctcaat tggaccaaaa ctggcatctt
     6301 ggtttgattg ctacaggaga tagttttgtt gcaggaaatg acaagataga agcgattaag
     6361 tcccatttcc cagaagtttt agccgtggag atggaggggg cagctattgc tcaagcagcg
     6421 catgccctca atctcccagt cttagtcatc cgagctatga gtgacaatgc caaccatgaa
     6481 gcaaacatct tttttgatga gtttattatc gaagctggac gtcgctctgc ccaagtcttg
     6541 ttgacctttt tgaaggcttt agattaagcg gaaatttgac agtttttcta gcttatgata
     6601 agatttaagt aaagaaaagc tagaaaacgt ttcagaggat attatgagta ttgaaatgac
     6661 cgtcagtgag attgcagagg tcttaggatt atctcgccaa gcaatcaata accgtgtcaa
     6721 agaattacca gaagaagaca cagataaaaa tgacaaaggg gtaacagttg ttaccagaag
     6781 tggcttgatt aagctagaag aaatctataa aaaaacgatt tttgaagatg agcctgtcag
     6841 tgaagatgtc aaacaacgtg aactgatgga gattctagtg gatgagaaga atgcagaaat
     6901 tcttcgtctt tatgaacaat taaaagccaa ggatcgtcag ttatcagaaa aagacgagca
     6961 gatgcgtatc aaagaccgtc agattgctga gaaagacaaa caattagatc agcaacaaca
     7021 attgaccctt caagccatga aggatcaaga aaatcttaag ctagagctgg accaagcaaa
     7081 agaagaagtc caatccacta aaaaaggctt ttttgctcgt ttatttggag gataatcaag
     7141 gagctgttta ggttaaatgc taacctgatg gcttcttttg tttgtgaata gtatagttga
     7201 ttagaacaga gacagtagag gagaggtaca gaatggaacg attagaagat tacattgctt
     7261 ttgatttaga atttaatcag cacgaaggac aaacacactt gattcaagta tcggcagtgc
     7321 gttttagaga tggtcgggaa atcgatgcct atgattccta tgttcatact agcgcccctc
     7381 taaaaagctt tatcaatggt ttgaccggga ttacagctga gaccttaaaa gatgctcctc
     7441 cagttgagaa agtcttagaa gaattccaag agtttgtggg ttctttgctg atgataggct
     7501 acaatgctgc taaaagtgat ttacctattt tagcagagca tggtttagac tatagccaac
     7561 agtacaaggt ggatttgtat gatgaagctt ttgaacgtcg gagttcagat ttacatggta
     7621 ttgccaatct caaattgcaa actgttgcct catttcttgg atttcaaggt cagtctcata
     7681 atagtttaga ggatgctcgg atgacggcgc gtgtttacga atccttctta gagtcggatc
     7741 aagctaggga attgttagag acagaaagta gcttatcaaa caatcctttt ggtggcttgg
     7801 acttgtctca attatttgac taggagtgcc tatgaaacct gaaaaaccta aaaaattggg
     7861 tcttaaacag atttacctca atcttgataa aatcctcttt ctatttttct tgatttttat
     7921 gatagtggag tatttgtggg taccattcaa ttcatggttg gctagcttct tattagatca
     7981 gacaggttat ctttttattt cctataacaa tgtcttagcc atccttactc attctccatt
     8041 gattagtcta gcttttttag ccttgattgt aattaacctt ctggttgctt attttcaaat
     8101 aggactcctc tttattggtg ctcgtcatct cctctataat gaaaagcgaa ggctaattga
     8161 gtatatccgt aaggttttcc atgaaagttt tggatttatg aaaaagctaa cttgcgctaa
     8221 agccttgttt atctttttct atatagctat gctttttcct tttatacgga aaatgctgaa
     8281 gatttattat cttaataaaa tcgtcattcc agagtttatc caagcttatt tagaagataa
     8341 atattggatg tggtggctga gcatcctact tctatctctt atctttctct atgtctctgt
     8401 cagattgatg tttgcccttc cgaagattgt atatgatcag ctgactgttc gagaagcagt
     8461 gatgtttagc ttggagaaaa ccaagaagag agttactttt tatgcttgga atttatttta
     8521 catcttgctc aaagcacatt tattatttta tcttcctttg attcctttat tattggctca
     8581 aactttggta gatgatatca ctcagagaga gtctctgatt cttggtattt tgaattttgt
     8641 cgtgattaaa aatctctatt atatggctct gacttatttt ttggttaaat ttgtttcatt
     8701 tttgacagga aaggagctgg atatgcttcc taggagagaa aaagatcaca tcgtgcgatg
     8761 gggtgtcatg acttgtgcca gtcttttctt tgccttagaa ggttatattt atctggaggc
     8821 tcccatggtt catctacctc aacttatttc tcaccgaggg gtttccaatg caaatgggat
     8881 tcaaaataca gtagagtcct tggaaactac agcacaactc aaaccagact tggtggagac
     8941 ggatgtgcag gaaacaaaag atgggcagtt tgtcatgatg catgatgcta acttgaaaaa
     9001 tctagcaggt atcaataaaa gtcctcaaga cttaaacttg gaggagctta aagggattga
     9061 tatttttgaa aatggctacc agactaaaat ttcaagcttt gaagattatc tcagtcgagc
     9121 caacgaactt ggtcaaaaat tactaattga aattaaaacc agtaaaaaag atagtccaga
     9181 catgatgaac cgctttttag cccgttatgc tgcaaagctc aagatttatg gacatcaaat
     9241 ccagtcttta gactaccatg ttgtcgaaaa agtaagacag tatgatgccg aactgccagt
     9301 ttatttcatc atgccctaca attctgtctt tcctaaaaca agagcgacag gatatactat
     9361 ggagtactca accttggatg aatattttgt aagcaaacta tggacaacgg atcagaaact
     9421 ttatgtctgg actgtcaatg attcagaagc tatcagcaaa tctcttcact taggagtaga
     9481 tggagtgatt acagatgatt tggaaaaagt tcagcgggaa atagaagtag cccaagaaga
     9541 tccagaatat acggatttgc tcttgaaaaa agcattggaa ttctttgaat tttagtaata
     9601 aaaaagagga acggttgttc ctcccatgat aggtaagtat tcttgtgaat gcttgctttt
     9661 ttgtggttgc aatccgttta cgagaattgt ttgtattgtt cttgtttcaa tttactttat
     9721 agtgctttga agttggaatg ctacactata acttctaaaa tatagtaaaa tgaaccaaaa
     9781 atagtacaca atgtggtata atcttcttat ggcatattca atagattttc gtaaaaaagt
     9841 tctctcttat tgtgagcgaa
//
LOCUS       DAVGJJ010000075         9547 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000075 DAVGJJ010000000
VERSION     DAVGJJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.075"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            455..1498
                     /locus_tag="VVE35_001616"
     CDS             455..1498
                     /locus_tag="VVE35_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU3061776.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1665..3752
                     /gene="topA"
                     /locus_tag="VVE35_001617"
     CDS             1665..3752
                     /gene="topA"
                     /locus_tag="VVE35_001617"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU3061777.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAYILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3973..4332
                     /locus_tag="VVE35_001618"
     CDS             3973..4332
                     /locus_tag="VVE35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU3061778.1"
                     /translation="MRIIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4425..5057
                     /locus_tag="VVE35_001619"
     CDS             4425..5057
                     /locus_tag="VVE35_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU3061779.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGVLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            5101..5757
                     /locus_tag="VVE35_001620"
     CDS             5101..5757
                     /locus_tag="VVE35_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU3061780.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5776..6408
                     /locus_tag="VVE35_001621"
     CDS             5776..6408
                     /locus_tag="VVE35_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU3061781.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEETINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6436..6870
                     /locus_tag="VVE35_001622"
     CDS             6436..6870
                     /locus_tag="VVE35_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU3061782.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYAINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6882..7918
                     /gene="leuB"
                     /locus_tag="VVE35_001623"
                     /pseudo
     CDS             6882..7918
                     /gene="leuB"
                     /locus_tag="VVE35_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7915..8184
                     /locus_tag="VVE35_001624"
     CDS             7915..8184
                     /locus_tag="VVE35_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU3061783.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGIVTTLVALYFIWR"
     gene            8188..8547
                     /gene="leuD"
                     /locus_tag="VVE35_001625"
                     /pseudo
     CDS             8188..8547
                     /gene="leuD"
                     /locus_tag="VVE35_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
     gene            8766..8909
                     /locus_tag="VVE35_001626"
     CDS             8766..8909
                     /locus_tag="VVE35_001626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061784.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPDVVLCGSMDELRALAQLNPKIE
                     AFY"
     gene            9009..9182
                     /locus_tag="VVE35_001627"
     CDS             9009..9182
                     /locus_tag="VVE35_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061785.1"
                     /translation="MTDVRGTSCFVIKFGKAGKQLAAKLWEEGKMVYASSSSMIKRLK
                     LAMRARCNGVYGR"
BASE COUNT         2965 a         1673 c         2118 g         2791 t
ORIGIN      
        1 aatttagtgt aattgagaaa ggagaaatga ttgtgattga tgttggctag gttatgttca
       61 atgattccta ccgtctcaaa tcttgtcagt aaggaaaaat aaattcttca aaagtagaga
      121 ttacaaggct tgtttaagaa agaattcaaa gaccttgaca aataaaaata aaatggttat
      181 tataagaaat ggtctaaaat agatgatgat acttttcgaa aatcttttta cgtcagctca
      241 gctttgcctt gctgtgtttt gagcaagcta cggttagctt ccgagtttga ttttcattta
      301 ctagaaatga aactgatgag agatatcagt agacatttga gtcaggatat tatggaaaat
      361 gataaaaaga gctcgtgaga ttggcatatc agactactaa agtattgagt ttgttaggat
      421 tttagcgact agttagctgg gaaaggaaga tattgtgaca aataataaac tgtattcgtt
      481 gatagaattt agaaataaaa tatatgaaga attagaactt tccagaagtg atttagcgat
      541 tttactatgt gccatgctta tcgcctctat cggattaaat atggattcga ctcccgtgat
      601 tattggagcc atgttaatct ctcctttgat gacacctatt ctgggagtgg ggctctctct
      661 agctatattt gattttaaat tgttaagaaa atcttttaaa atattagcta ttcaaattct
      721 tgccagttta atagcttcaa cactttattt ttatctttct cccatttcgt atgctagttc
      781 ggaaattgtt gctagaacct ctccgactat ttgggatgtt ctcattgctt ttgtaggagg
      841 gatagcaggt attattggtg ctaggaaaaa agagaccaat aatattgttc ctggtgttgc
      901 tattgcaacc gccttgatgc ctcctctttg tacagtaggt tatgctattg cttctgctaa
      961 tctaaaattt atcataggct cctcttacct attcctcatc aattgtagct ttattgtcat
     1021 tgcgacttat ataggtgtta ggttgatgat ggttaagaaa cattatttta aagataatga
     1081 agaagactct aaaatgcgta ggattttgct tctagttgct gttttgctga tgattccgag
     1141 tttcatctct gcaacgactt tagtgagaga aacgttgaaa aaagagtccc ttaagaaatt
     1201 tatatcagag cagtttcagg ggtataatat tttgaaaaaa acctattcta aaaagactca
     1261 taccctaaag ctaaccattt caggaaatta tttgacagaa gaagaactcg atatgatttc
     1321 aagtaagaga ggtgactatg gtttaagtga tgtttctgtt caagtttcac aattgtctga
     1381 ttcagaacaa cttagtaagg aagagctggt ggagtatttc ttccagtata tcaaggataa
     1441 ggaagcaaaa gaaaaggaga aagccaataa atttgataca gagtctgagg agcaataatt
     1501 tcttgagaat agctggtttc tctcgtgagt cttctatgta tatcaaagga agactgaggt
     1561 tttaagtata aaactttttt tctattatag tagctaagtc ttgacagggt tcaaaaaatg
     1621 gtttacactt tataaagttt attactttga aaaggtgtga tactgtggct acggcaacaa
     1681 aaaagaaaaa atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct gctaaggcca
     1741 agacgattga aaaatatcta ggcagaaact acaaggtttt agccagtgtc gggcatatcc
     1801 gtgatttgaa gaaatccagt atgtccgtcg atattgaaaa taattatgaa ccgcaatata
     1861 ttaatatccg aggaaaaggc cctcttatca atgacttgaa aaaagaagct aaaaaagcta
     1921 ataaagtttt tctcgcgagt gacccggacc gtgaaggaga agcgatttct tggcatttgg
     1981 cctatattct caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat gaaatcacca
     2041 aggatgcagt caaaaatgct tttaaagaac ctcgtaagat cgatatggac ttggtcgatg
     2101 cccaacaagc tcgtcggatc ttggaccgct tggtagggta ttcgatttcg cctattttgt
     2161 ggaagaaggt caagaagggc ttgtcagcag gtcgcgttca gtccattgcc cttaaactca
     2221 tcattgaccg tgaaaatgaa atcaatgcct tccagccaga agaatactgg acagttgatg
     2281 ctgtctttaa aaagggaacc aaacaatttc atgcttcctt ctatggagta gatggtaaaa
     2341 agatgaaact gaccagcaat gacgaagtca aggaagtctt gtctcgtctg acgagtaaag
     2401 acttttcagt agatcaggtg gataagaaag agcgcaagcg caatgctcct ttaccctata
     2461 ccacttcatc tatgcagatg gatgctgcca ataaaatcaa tttccgtact cgaaaaacca
     2521 tgatggttgc ccaacagctc tatgaaggaa ttaatatcgg ttctggtgtt caaggtttga
     2581 ttacctatat gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat gaggcggcaa
     2641 gcttcattac ggatcgtttt ggtagtaagt attctaagca cggtagcaag gtcaaaaacg
     2701 catcaggtgc tcaggatgcc catgaggcta ttcgtccgtc aagtgtcttt aatacaccag
     2761 aaagcatcgc taagtatctg gacaaggatc agctcaagct atataccctt atctggaatc
     2821 gttttgtggc tagccagatg acagcggccg tttttgatac catggctgtt aaattgtctc
     2881 aaaaaggggt tcaatttgct gccaatggta gtcaggttaa gtttgatggt tatcttgcca
     2941 tttataatga ttctgacaag aataagatgt taccggacat ggttgttgga gatgtggtca
     3001 aacaggtcaa tagcaaacca gagcaacatt tcacccaacc gcctgcccgt tattctgaag
     3061 caacactgat taaaacctta gaggaaaatg gggttggacg tccatcaacc tacgcgccaa
     3121 ccattgaaac cattcagaaa cgttattatg ttcgcctggc agccaaacgt tttgaaccga
     3181 cagagttggg agaaattgtc aataagctca tcgttgaata tttcccagat atcgtaaacg
     3241 tgaccttcac agctgaaatg gaaggtaaac tggatgatgt cgaagttgga aaagagcagt
     3301 ggcgacgggt cattgatgcc ttttacaaac cattctctaa agaagttgcc aaggctgaag
     3361 aagaaatgga aaaaatccag attaaggatg aaccagctgg atttgactgt gaagtgtgtg
     3421 gcagtccaat ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt agcaatttcc
     3481 cagattgccg tcatacccaa gcaatcgtga aagagattgg tgttgagtgt ccaagctgtc
     3541 atcagggaca aattattgag cgaaaaacca agcgtaatcg cctattctat ggttgcaatc
     3601 gctatccaga atgtgaattt acctcttggg acaagcctgt tggtcgtgac tgtccaaaat
     3661 gtggcaactt cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt gtttgtagca
     3721 aaggcgacta cgaggaagaa aagattaaat aagaggaggg gcccgaaagt aaatttcagg
     3781 ctccttttgg ttagaacttg acaaaataca tcattttatg cgaaactaga agaagattat
     3841 tttatactca atgagaatca aagagcaaat tagaaagcta gtcgcaggtt gctcaaagta
     3901 ctactttgag gttgaagatg gaagctgacc tggtttgaag agattttcga agagtattaa
     3961 ctaggagaag ttatgcgtat tatctatcta attattggtt ttttatcgct ggccttggct
     4021 attgttgggg ttgttttacc cttgttgcct acaacacctt tccttttgtt gtctattgct
     4081 tgtttctcca gaagttccaa gcgattcgaa gattggcttt atcataccaa gctctatcaa
     4141 gcatatgtag ctgattttcg tgagaccaag tctattgcgc gtgaacgtaa gaaaaaaatc
     4201 atcgtatcca tttacatttt gatggggatt tctatttatt ttgcccctct cttaccagtc
     4261 aaaatcggtc tgggagcttt gaccatcttt atcacctatt atctcttcaa ggtcattcca
     4321 gacaaagaat aattaaaaca gtagttattt cccttgataa aattgaaagc atattcataa
     4381 caatatgata taataaattt gaagtaatat tcaaggagaa tcaaatgatt tacgaatttt
     4441 gtgctgaaaa tgtgaccttg cttgaaaaag cgatgcaggc tggagctcgt cggattgaac
     4501 tctgtgataa tctagcagtt ggtgggacaa cacccagcta tggagtgact aaggcagcgg
     4561 ttgaactggc agctaactac gatacaacca tcatgaccat gattcggcca cgtggtggtg
     4621 actttgtcta taatgaccta gaaattgcta tcatgctaga agacattcgt ttgactgctc
     4681 aggctggaag tcaaggggtt gtatttggag ttttaactgc tgataaaaag ttggataagc
     4741 ctaatctgga aaagttaatt gctgcatcaa aaggaatgga aattgtcttt cacatggcct
     4801 ttgatgaact aagtgatgaa gatcaaccgg aagctattga ctggctcagt caagccggtg
     4861 tcactcgtat cctaactcgt gctggtgtgt ctggcgactc cttagaaaaa cgttttgttc
     4921 actatcacag aattttggag tacgctaaag gtaaaattga aattctacca ggtgggggga
     4981 ttgaccttga caaccgtcaa acctttattg accaggtggg ggtaacacaa ttgcatggta
     5041 ctaaggttgt tttttaaaaa atagaaagga actgctagcc ttgggtagca gttttcactt
     5101 atgtttgaaa tttttaaatc ctatcaattt aatcaagaaa aggctcatga ttatggtttt
     5161 atagaaaata gcgaagtctg gacatatagt tgccagattt tgcaaggtga ctttgtcatg
     5221 actgtgtcca tcactgctga taatgtgaac tttcaagtct ttgaccaaga gactggtgac
     5281 ctctatcctc acgttcatat ggaaagcatg aggggaagtt ttgtcggaaa tgtccgtgag
     5341 gcttgtctgg agattcttta ccagattcgg aaggcttgtt ttgatgtgca agattttatc
     5401 tgtcatcaga ctaagcgtat catgactcaa gttcaggaaa agtatggaaa ccagttggag
     5461 tatctgtggg aaaaatcgcc tgatacagct gtattgcgcc atgaaggcaa tcaaaagtgg
     5521 tatgccgtct tgatgaaaat ctcttggaat aagctggaaa agggcagaga aggacaagtg
     5581 gaagcagtca acctcaagca tgaccaagta gctaatttgc tttcacaaaa ggggatttat
     5641 ccagccttcc atatgagcaa gcgctactgg attagtgtgt cccttgatga tactttatca
     5701 gatgaagaag tactggaatt gatagaaaaa agttggaact taacctctaa aaaatgaaat
     5761 attttaataa ttttcatgaa ctttcaatta gctaaatatt ctttactgaa gaaattttca
     5821 gaaaatatag gatttaccac accagaggaa tgtggtgcca tcttcaaata cctgattgag
     5881 aatgtcaaga cggatcgtca gatcatctat tcgcctcact gtcatgatga cctcggaatg
     5941 gcagtggcaa atagccttgc tgctgtcaag aatggtgcag gacgtgttga agagactatc
     6001 aatggtatta gggagcgagc tgaaaatgct gctttggaag aaattgcagt ggctctcaat
     6061 attcgccaag attactacca agtagaaacc agtattgtcc taaatgagac catcaatacg
     6121 tcagaaatgg tttctcgctt ctctggtatt ccagttccta aaaacaaagc cgtcgttggt
     6181 ggcaatacct tctcccacga atctggtatt caccaagatg gagtccttaa aaatcctctc
     6241 acttatgaga tcatcacacc tgaattggtt ggtgttaaga ttccgcttgg aaaattatct
     6301 ggtcgccatg cttttgttga gaaactgaga gaattggccc tagattttac agaagaggat
     6361 atcaaaccac tctttgctaa gttcaaggca ctggccgata agaagtaaga aatcacagat
     6421 gcagatactc gagctttggt agctggaacc atggttgaaa atccagaagg cttccacttt
     6481 gatgatttac aacttcaaac tcatgcagat aatgacattg aagcgctcgt tagcctagcc
     6541 aatatggatg gtgagaaagt cgaatttaat gcgacagggc aaggttccgt tgaagcaatc
     6601 tttaacgcta tcgataagtt ctttaaccaa tctgttcgtt tggtgtccta cgctatcaat
     6661 gcggtaacag atggaatcga tgcccaggat cgggttttgg tcactgttga aaacagagat
     6721 acagaaacca tctttaatgc agcagggctt gattttgatg tgttgaaggc ttctgctatt
     6781 gcctatataa acgctaatac ctttgttcaa aaagagaatg caggtgagat gggacgcagt
     6841 gtttcttacc acgatatgcc tagtgtgtaa aggagaaggc tatggcaaag aaaatagtag
     6901 ctctagtagg agacggaatt ggcccagaaa tcatggaggc tggtttagaa gttctggagg
     6961 ctctagctga aaaaacaggt tttgactatg agattgacag acgaccgttc ggaggtgcag
     7021 atattgatgc agcaggacct cccttacctg atgaaaccct taaggcaagt agggaagtag
     7081 atgctatcct actagcagct atcggtagtc ctcagtatga tggagcagcg gttcgccctg
     7141 aacaaggcct gatggctctc cgtaagaact caatctttac gctaatattc gtcctgtaaa
     7201 aatctttgac agtctcaagt atttgtcacc actcaaaccg gaacgaattt ctggtgtaga
     7261 ctttgtcgtg gtgcgtgaat tgacaggcga gatttacttt ggagatcata ttcttgaaga
     7321 gcacaaagcg cgtgatatca acgactatag ctatgaggaa gtggagcgga ttattcgcaa
     7381 agcctttgaa attgcaagaa atcgcagaaa aatcgttact agtatcgata agcaaaatgt
     7441 tctagcgacc tcaaaactct ggcggaaagt agctgaggaa gtcgcacagg atttcccaga
     7501 tgtaaccttg gaacaccagc tggtagactc agctgctatg cttatgatta ccaatcctgc
     7561 taagtttgat gttattgtaa cggagaatct ttttggagat attttatctg atgaatcaag
     7621 cgtcttatct ggtacacttg aggttatgcc atcagccagt cattctgaaa atggaccaag
     7681 tctctatgaa cctattcacg gttcagcacc tgatattgca ggtcaaggaa ttgccaatcc
     7741 tacttccatg attttatcag ttgccatgat gttgagagat agtttcggag gttatgagga
     7801 tgcagagcgt atcaaacgtg ctgttgagac aagtctggcg gcaggaattt taacgagaga
     7861 tatagggggt caggcttcaa caaaggaaat gacggaagct attattgcaa ggttatgaag
     7921 ttagacgaaa aaattactct agtccttttg atttggaatg tcatcatttt cttgatttat
     7981 ggcattgaca aattcaaggc aagaagaaga gcttggcgca tcccagagaa aatcttactc
     8041 attttagccc ttacttgtgg tggttttggg gcctggttag ctggaatcac ttttcaccac
     8101 aagactcgaa aatggtactt taaaacagtt tggtttcttg ggatagtgac cacactagta
     8161 gccttatatt ttatttggag gtaatggatg gcagggtctt cgagggaaca cgctgcttgg
     8221 gctctagtgg actatggttt taaggtcgtg attgcaggat ctttcggtga cattcattac
     8281 aataatgaac tcaataatgg cctgttgcca atcgttcagc ctagataggt tagagagaaa
     8341 ctagcccagc taaaaccaac cgaccaggta actgtggact tggaacaaca aaaaatcatc
     8401 tcaccagttg aagaattcac cttcgagata gatagcgagt ggaaacataa actcctaaat
     8461 agtttggatg atatcggtat taccttgcag tatgaagagt tgattgctgc ttatgaaaaa
     8521 caacgaccag cctactggca ggattagaaa aaatagaaaa ggaaatatag taaactgaaa
     8581 taagatgtaa acaaatgaat tggagcttaa catccatttc cagcaatttt ttagaaacta
     8641 cagtggacta ttctggattc aacacattat aaaattatga caaaacacat tcacaagaag
     8701 gctacgacat tttaaaaggt gagggcggat gtatcgtttg ccctactaaa gttggttaca
     8761 ttatcatgac cagcgacaag gcaggacttg agcgtaagtt tgcagccaaa gaacgtaagc
     8821 gtaacaaacc agatgttgtt ctctgcggta gcatggatga acttcgcgct ttagcgcaac
     8881 tcaacccaaa aattgaagca ttctactaaa aacattggga tgaagatatt cttcttggtt
     8941 gtatccttcc ttggaaacca gaagcctttg aaaaactcaa agcatacggg gatggccgtg
     9001 aagaacttat gactgatgta cgtggtacta gctgttttgt tatcaagttt ggaaaagcag
     9061 gtaaacaatt ggctgccaag ctttgggaag aaggtaaaat ggtctacgcc tcatcttctt
     9121 caatgataaa acgattgaaa ctcgctatga gagcaaggtg taatggtgtc tatggtcgat
     9181 aaggacggca aactcatccc agaacaagga ggagcacgtt caacttcacc agctccagtt
     9241 gtgatccgta aaggacttga cattgatata gtaaaatgaa gtaagaacag gacaaattaa
     9301 tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt
     9361 tcaatctact ataaaatcat gatgcacctg tcagatactt ttaactcatg ggactaccgt
     9421 cagggtgagt attattaaga taaaaaaaga agtctagtgt tatgagatat taaagctcct
     9481 aacactgggc tttttgttta gaatttcttt tctttttcta taggatatgg tattctatgt
     9541 agaaaat
//
LOCUS       DAVGJJ010000076         9546 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000076 DAVGJJ010000000
VERSION     DAVGJJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.076"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(116..1138)
                     /gene="cbpF"
                     /locus_tag="VVE35_001628"
                     /pseudo
     CDS             complement(116..1138)
                     /gene="cbpF"
                     /locus_tag="VVE35_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1157..1978)
                     /gene="cbpG"
                     /locus_tag="VVE35_001629"
                     /pseudo
     CDS             complement(1157..1978)
                     /gene="cbpG"
                     /locus_tag="VVE35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
     gene            complement(2059..2298)
                     /locus_tag="VVE35_001630"
     CDS             complement(2059..2298)
                     /locus_tag="VVE35_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061786.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDCSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2448..2714)
                     /locus_tag="VVE35_001631"
                     /pseudo
     CDS             complement(2448..2714)
                     /locus_tag="VVE35_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2739..3050)
                     /locus_tag="VVE35_001632"
     CDS             complement(2739..3050)
                     /locus_tag="VVE35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU3061787.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2975..3206)
                     /locus_tag="VVE35_001633"
                     /pseudo
     CDS             complement(2975..3206)
                     /locus_tag="VVE35_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3207..3839)
                     /locus_tag="VVE35_001634"
     CDS             complement(3207..3839)
                     /locus_tag="VVE35_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061788.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(3853..4848)
                     /locus_tag="VVE35_001635"
     CDS             complement(3853..4848)
                     /locus_tag="VVE35_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3061789.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4845..5543)
                     /locus_tag="VVE35_001636"
     CDS             complement(4845..5543)
                     /locus_tag="VVE35_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU3061790.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5621..6631)
                     /locus_tag="VVE35_001637"
     CDS             complement(5621..6631)
                     /locus_tag="VVE35_001637"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU3061791.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(6615..7622)
                     /locus_tag="VVE35_001638"
     CDS             complement(6615..7622)
                     /locus_tag="VVE35_001638"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU3061792.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMERERKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7609..8562)
                     /gene="mvaD"
                     /locus_tag="VVE35_001639"
     CDS             complement(7609..8562)
                     /gene="mvaD"
                     /locus_tag="VVE35_001639"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU3061793.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8544..9422)
                     /gene="mvk"
                     /locus_tag="VVE35_001640"
     CDS             complement(8544..9422)
                     /gene="mvk"
                     /locus_tag="VVE35_001640"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU3061794.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
BASE COUNT         2753 a         2264 c         1676 g         2853 t
ORIGIN      
        1 acttttgggg ggcagtacag aaaaaccttg atactatgcg ttttttgatt gtgaagactt
       61 ttatccacct attatacaaa gttaagtatc catcacagtt tacaattagc cttcattact
      121 taacccattc accattatag tttaagtagt aaccacctac tgtggtatta acagctaaag
      181 cacctgaatc ataggcatag taccagttac cattgacttg gaaccagcct gttttcatat
      241 ctccattact tgcatttaga tagtaccaag tcgaaccttc ctgataccaa ccagttgcca
      301 tagctcctga tgaacggaga tagtaccatt tgttcccaag gttttgccag ccagttctca
      361 tatcaccatt ttcagcatct agatagtacc aagttgagcc ttccttatac cagccagttg
      421 ccatagctcc tgacgaatgg aggtagtacc acttattacc tagatattgc caacctgttt
      481 gcataatgcc agttgctgga tctagatagt accatggagc tgcttttagc ttctcttcat
      541 cataggtaag agggtaatcc ttaacccaac cacgtgctag ctctccaacc gccaatctgt
      601 tgatgcgcga atcaaagcca ccatccttct gtaaataata ccaatgaccc tcttcgtaaa
      661 tccaaccagt ttttaaagta tgataactac gctgatcttc aaaataatag actcatttct
      721 ctgctgggct atcatattct tccccatgat gtttgttggt attcgtagca gtttttgctt
      781 ctaaaacttg cttgccaaca aattcttgta atacaccatc ttgaccaaaa taaaaccaat
      841 ctggtctaag agaaatctct attcttggag aaggaccaat cgtaaccccc ttgtgtggag
      901 caggtatata ttgccagccg acaaccatct ctccagatag agaatcaaaa taatagtact
      961 tcccatctat cactctccag taggtttctt ttaggtcccc ctttttgtaa taggttctac
     1021 cgttttcttg gacaaactgc catccttcag aatcatctgc aaatactgta ctggtcccta
     1081 gcaaaccgaa gaaaaatgtg gctagtgcga cttgcatcat ttttttcaaa agcttcattt
     1141 tatatatcct ccaacatcaa atccactcac cagatgaagc gaagttataa actttaccat
     1201 cgatagtttg gctacctgta accattgctc catttgaatt gagataatac cagtttccat
     1261 ttactttttg ccaatctgta agcatcttac cggaactgtc taaataatac caagtatcat
     1321 ttatctcctg ccaacccgtt tgtttatcat gttgtctata atagtaccaa ctaccattta
     1381 ttttcttcca tccttcaaga gagtaacctt taagaaccga ataaataaat gacaaatttc
     1441 gttcatttaa tttaactgca ctgttaattt gattagctcc atctcctaaa gtatgcactc
     1501 ctactacacg gtgactagca tcataaactg tagatccact agacccctct aaagtatcaa
     1561 cttggtaatc caagaacatg ccatcatcac ttaaaacttg ttttttatct gtatacattt
     1621 gatgaatttt aaaattatat gatggatagc ctgtgatagt cacagttatt cctgtcaaat
     1681 ttttttgact agtaggaaga cccaaagtcc ctaattttgc accaatgggc ttttctagaa
     1741 ttaataaagc caagtcatat tcccttgcat tcttagaatt taaatttcta aattccttca
     1801 aataacgaac ttcctttact ttgatctttc caaatggttc ttaacttgga ctaacagccg
     1861 gaagaacata aatatcatcc gcttctttcc catagtcatg tctgtagtag ttgtgagccg
     1921 ctgtaattaa cacattatct ttaataaaat tcgcacttcc cctcccttct acattcattc
     1981 catcggctac tccataatac ttagataaaa ccatagctga agtcgaatac ggatactgta
     2041 aagtattatc aattttaatc aaatcatcat taccgataat acttctgatt gcttttggta
     2101 gtatgaacca tatgttggtg aaatctcaga taatgaacaa tcattagatc ctggaccttt
     2161 ttctagtgtc tcacttacct catattcttc acctgtacta gaaataacac tcaaagcaga
     2221 tactgtcgat aactggctag ccaataaagt actcgcaata attgaaatac ccaatttttt
     2281 ataaacagtt ttcttcatta ttgtatcctc ctaatgtaat tatagcgtac tattctaaat
     2341 ttcttaatct actatagaat caagaaatct accaccttct ttaaataccc tccattatca
     2401 cataaacagg taaacttttc aattaatgac tgcgcttttc aatcacgcta gaggtactta
     2461 cttgcttctt tgatactaag ttcagccatt ctttccttgt ttttctcaat aaagcatgtt
     2521 acccaagtgg gattcgtttt ggagtagtct cgcagagtcc agccaatagc tttattgata
     2581 aaaaattctg tttggttcaa gttatgaagt agaatctttt ccattaattg agtattggtc
     2641 ttctcttttc ttaacaactg gtggtcaata gcgacacgtc tcagccagat attatctaat
     2701 aggctccatt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcagattg
     2761 ctcaaaacac tgttttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag
     2821 agtattaaga ttatttcttc tagttcaggg tgttcataca ccaaactccc tactactcga
     2881 tctaggatat ctaccgtgtc ccaccaggat tttgtcacga ctaactgctc tagcttaggc
     2941 aaatcggttt cctttagata agactgcatt gctttcaaat agttagcagc cacatattgg
     3001 tattttctag gctccttttc ccagcaagtg tctgcaaaat cccaatcgat aatctttgtt
     3061 tttttcgctt ctggaaaata tttttataga gtttatttct ttcaacctag aaaagaaaat
     3121 tgatggcgca tataggcttc catgggcctt gcttttttag agtcttttgc tgcttctagc
     3181 tcctcaagta aatctgctaa actcatctaa aactcctctt gccccaccaa atggtgctga
     3241 aaggcgtaga cagccgcctg ggtacgatcg ctgacttcaa gtttggcaag aatgttggac
     3301 acgtgggtct tgaccgtctt gagagagata aagaggtcat ctgcgatgcg ctgattttcg
     3361 tagcccttgg cgatgagttg gagaacatct cgctcacgcg cagtcaattc ttcatgaagt
     3421 tccatatgat tgcggtggta ttcaaccttc ttgctaacct cttgctcaat ggccagctcg
     3481 ccagcagcta ccttactgac ggcatgaagc aattcatctg cactagaagt cttgagcata
     3541 tagcctttgg caccagcatc taagactggc atgatttttt cattgtccaa ataagaggtc
     3601 acaatcaaaa tcttggcttc aggccattct ttgaggatag ccaaggtcgc atcaatccca
     3661 ttcatctcag gcatgacaat atccataaca atgacatctg gacgcagttc caaggccaag
     3721 tcaatccctt gagacccgtt ggacgcctca cccacaactt ctacatcgtc ttggaggtca
     3781 aagtagcttt tcaagcccaa acggaccatt tcatggtcat ctactagtaa aattttcatc
     3841 tttactcctt tatcattcct tatctaacag gggaatacgg atatcaactg ccaacccttg
     3901 cttgggagct gttaataact gaaccgtccc tgccatatct tcaacccgct ccttgatatt
     3961 tcgcagtcca taactcaagt cgtctaagct ccctaactgg aaaccaatcc cattgtccac
     4021 caccttcagt tgcaattcga catctgtctg atagaggtag acatctaggc aagatgcctg
     4081 ggcatggcgg agcgtattgc taatcaactc ttgcaggata cggaagatat gctcctcgat
     4141 tttcttaggc aatttcgtca tattctgctt gagactaacc ctaagatcac tcttgtcctc
     4201 aagctctttt aagagaattt gaatcccttc tatcaagctc ttctgctcca gttcaactgg
     4261 tcgcaaatgc aagagcaaaa cccgcaaatc cttctgggct gtttctaaaa tagctgtgac
     4321 actctgcaac tgggtctgca tcttttctct atccaatttc aaagcctgct gactgatacc
     4381 cgataaaatc atgtgggccg caaacaactc ctgactgact gtatcgtgca aatcccgagc
     4441 aattcgcttc cgttctttct cgatgatttc ctcttcctga gcaaggctat gattttcagc
     4501 tttttgaaga gcttctgtca aaaggttaag tttacctgat aaggacttga aactggcatc
     4561 caaatctgga tctgcaacct gaaccacttc ttgccctgcc aataaacgct tgagattagc
     4621 ctgcattttt cttagagaaa gctcttcgat ccctcgccaa aacagggcta agagacaggt
     4681 catggacatg ctgaaaacca acaataaaaa gacaaatttt tctgtttttt cgacatcgtg
     4741 caaaaagata gaccagtcaa aatcaagtat ttccagcaag ctgtgggaga aaaaaaagac
     4801 aaacaggaag gaggtgagag caataatgac ataggcttgt tttttcatcc tctaaccacc
     4861 tccacatcac caatcatagt ggtcaagaaa atcttgacac tcttgttact cttgagatag
     4921 tcttttgttt cttgatgata gtgttcattg cggagggctc gcttgggctg gttgaaaaaa
     4981 gtcaaatccc catagagaca gttaacgctg agactgactt ccacatctac aggtacgatg
     5041 attttggtcg ttcctaccat ctttctgagg ataatgacat tgtcatgatt ggttaagatg
     5101 accctctcca gatgaatagt gtccttgccc atgaagcgaa agagattgat atcatcgaat
     5161 tggcaagtct ggtagcttga aaaatgatga agatttccaa accaacgatt tttctccttc
     5221 ttaaccgtca cgacctcttc aaaaaccaaa ttggtctgct ctttttcctg gttcatcatc
     5281 gggtaaagaa gaaagaggct atagataacc gcaacaaaaa tagctagaat cacaaaagga
     5341 ttgagcataa cgatgaaaaa gaagagaatg gttaccgcta ctaaaagaag attatttccc
     5401 tctttaccag tgtagtagcg aatcaaaagc aaaaagagga atagtatcag cagaaaacgc
     5461 gaaaaatgct ctgataccat caaaatcaga gctcctgtca gaagacaggc ttcgataaat
     5521 aaaaagattt taaattttct cataggttca tcctctccct tctattttat cacaattcaa
     5581 aaaagtcacc tcagtctgag gatggaaaaa aaggcgctgg ttacgccttt ttcatctgat
     5641 catttgcttc ttttaatttt ccataaagaa gatagtctac tttttgtaga tctgctatgg
     5701 tggcacagtt aagggaacac ataatcaagc gtagatctgc tttccagcct tggacaatgc
     5761 caatcacttc ttcaactgtg taggtttcaa ccaattccag aacggttcgt gacaatccca
     5821 cagccttagc accaaaaacc aagcacttaa tcatatccag cggattccga acccctccac
     5881 taaccaagag ttcgacctta tctttccatt cttgggcatt gagaagggcc tgcatggtag
     5941 actgacccca ttgattgagg taatcacgct ggccactacg acggttttcg atataggcaa
     6001 agctggtgcc accacgaccc gataggtcca ctgtacgaac accgaattca taggctcttt
     6061 cgattgtctt ggcatccatt ccaaagccca cttccttgag gacaatagga acgggaattt
     6121 gcttgctata atctgctaaa tgcgattgcc agcttctaaa cttcctttct ccctcgggca
     6181 tgagtaattc ctgcatgaca ttgacatgca cttgcaatag aacaggattc atctcttcta
     6241 cagtctgaag tcctaactcg acaggcttgt ccaatccaat attggttcca aggaggagat
     6301 tgggatgact agacttgaca gaaaaagaat catccgttgg atttttgagg gctgcgctat
     6361 aagaacccgt tacaaataaa ataccacagg attccgccac ctgagccagc ttttgattga
     6421 tttctcttcc cttattactt ccaccagtca tggcattgat ataaaaagga aagtcccact
     6481 ttcgaccagc aaactctgtc gaaagatcga tttcatccag attgtaaaga ggcaaggaag
     6541 aatgaatcag ctccacctca tcaaagctat tataggaact tttctgctca agggcataga
     6601 ggatatgctc gtccttacga tttgtcgtca tgtcctatcc tttcttgata taagagctca
     6661 atccccagat cggcccaacg attttttaag gttttggttg attgcgcatc aaaactcagg
     6721 gcgatgccac agtcaccacc accagcacca ctactcttgg caacggcctg caaatcttga
     6781 ctggcttctt tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa gccttctaaa
     6841 agcttgctgg ctacttctac ttgatcgata atcttttctg atttcccctg ttccaaggct
     6901 tctaccagag aagtcaccgt ttcttttgag gaagttaaaa aattttgatt gatattttgc
     6961 ttgatttgct ggaccatgtg actcgataca gccacttcct tggtccatcc cactaagaaa
     7021 tcacattcta aagttggttt cacttgtgaa attgaaaagc cccaatcacg ctccagaact
     7081 gtcgccaagt tttcttcttc taaccaagca gccaccttct ggcgatcaaa tgactggtag
     7141 agaaccaaat cctctgccac aatacaggca aggtccccca tggaaccatt gtctcctcgc
     7201 ttgagcaaga cagcgctagt cagcttgaac aagagctcct gatcaacaga aacatcatac
     7261 agagccagta aagccttgac aaccaagaca acgacgctgc cactagaacc tagaccaaac
     7321 tttttccttt ctcgttccat tttgccacag atttttaggg aaaaaggtct taaattctga
     7381 ccgcgaacag cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa gctgtagtca
     7441 ggattaggcc ttaagtccac tgcgaaatca aacatatctg aatagatacg gtagctgtca
     7501 gaaaaagcaa tctcagccct catatagatg ggaatatcct ttatcaaagc taactgccct
     7561 ggctctaaaa tagcatattc acctgcccaa tagagttttc cgcaagtttt aacagcaatc
     7621 atcttgactc aaatcctttg tttttgacac aatcaagcga taacgctgac cgaaaatttc
     7681 tgataaatgc tccaagtctt tctcctgaca gaagacctta acattgggac cagcatccat
     7741 ggtaaagtag caggcctctc ctttctcacg aagctggcga acaaagtcca tagcctcata
     7801 agaggcatcc gtcagataag aaaaggctgg actagcagtc tttgtcgtag catgcatagc
     7861 cagagcattt ttctccgtta attctccaat cttggcaaaa tcattttcct tgagataaat
     7921 cagcatatcc tgatagtcct tctcagactg acgaacccag tcgtcaaaag tcgtcgaggt
     7981 ttccacacaa agtttcatcc cgtcacggct agagattggt tttttcttgt cctctagcac
     8041 caacataatc atagctagtt tcaagtctgt ctctacaggg taaatttctc cactatcctt
     8101 atcccaggct cctagtggtc cataaaaact ccgagaagaa gagcctgagg caaatttggc
     8161 ttcctgtgcc aactgacttc tatccaatcc aagcttgaaa taagcattac aagccttgac
     8221 cagggcggac aaaccactag aacttgagga caggcccgct gcagtaggca tattgttttg
     8281 agtatcgata cggacaaagc cctcaccagc tggacgataa cggtcaataa tcttactcat
     8341 cttggcatgc tcgacctcat tttgtagctg accattgatg taaaattcgt cagctgttac
     8401 attggctggt aaaggcgaca aggtcgtctc tgtatacata ttttccaaag ttagagaaat
     8461 actgctagta gcaggcacca tctctttttc ttttttcttt ccccaatatt tgataatagc
     8521 aatatttgcg taggaacgta ctgttacagg ctctctatcc atgtctgaac agctcctttc
     8581 tcttctaatc tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa ggctatgata
     8641 caacctccta gcccaccacc gctcatcttg gcacccagag caccatggct aagagtcgtt
     8701 tcaaccaaaa agtctgcctc agggctactg actccaattt cttttaaatg taaatgcgct
     8761 tgactgagga tttgtcccag tccttcagca tctttttgtg aaatcgcaac ttctgcttgc
     8821 tgggttaatt ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt attttgaacc
     8881 acttggatgg cttcacgagt atgaccataa acacccgtat cggcaatcac caaataggcg
     8941 gataaatcca tctcaagttc tgtaaatcct acgttcttga taaagcgaat aggttggtca
     9001 ctgagacagg tcttagcatc caaaccacta ggattcatat gggcaatcat ttcagctcga
     9061 ttgaccaaga tttctagtac atcatgaggc agatcagcct gatagtagtc aaataccgca
     9121 cgaatggccg ctatgctgat agccgctgac gaacccatcc cccgtttctc agggatagcc
     9181 gagtcaatct cacaacgaat gcaggcttct gtgatatcca aatactccag tgaggcataa
     9241 accgccatgg acaaggtatc ctcctcataa aggcgccaag gactctctgc aggaactacc
     9301 ttacaggtca cctccacctc caaaagaggc agggaaatgg caggataacc gtaaacgacc
     9361 gcatgttccc ctattaaaat tatcttacta tgtgcctgac cgacaccaac tttttttgtc
     9421 attttttcct tttactagac gaaaaaacgt cttatttttc atacaagtat taattctttc
     9481 ctatctattt tattatattt tcacaaaaaa agcgattgtt tccattcaca atcgcttctt
     9541 tcatta
//
LOCUS       DAVGJJ010000077         9457 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000077 DAVGJJ010000000
VERSION     DAVGJJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.077"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1487)
                     /gene="pfbA"
                     /locus_tag="VVE35_001641"
     CDS             complement(<1..1487)
                     /gene="pfbA"
                     /locus_tag="VVE35_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU3061795.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNV"
     gene            complement(2650..3876)
                     /locus_tag="VVE35_001642"
     CDS             complement(2650..3876)
                     /locus_tag="VVE35_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU3061796.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3919..4611)
                     /locus_tag="VVE35_001643"
     CDS             complement(3919..4611)
                     /locus_tag="VVE35_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU3061797.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFELLK"
     gene            complement(4784..6535)
                     /locus_tag="VVE35_001644"
     CDS             complement(4784..6535)
                     /locus_tag="VVE35_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061798.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(6525..8270)
                     /locus_tag="VVE35_001645"
     CDS             complement(6525..8270)
                     /locus_tag="VVE35_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061799.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLFLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8476..8772)
                     /locus_tag="VVE35_001646"
     CDS             complement(8476..8772)
                     /locus_tag="VVE35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU3061800.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8791..8928)
                     /locus_tag="VVE35_001647"
     CDS             complement(8791..8928)
                     /locus_tag="VVE35_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061801.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     P"
     gene            complement(8925..9382)
                     /locus_tag="VVE35_001648"
                     /pseudo
     CDS             complement(8925..9382)
                     /locus_tag="VVE35_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
BASE COUNT         2875 a         1998 c         1831 g         2753 t
ORIGIN      
        1 attacatttt ttgttacagt aatatctgat acttttccta aatattctgc actatctttt
       61 gcaactcgta tcgcttttgt tttaggatcg gcaatattaa atatattttc ggcgataacc
      121 acctgtttat ttaaatctaa taggtcttta gtgtttttat agctataagc atttactaaa
      181 gctgctccgc tttctcgata atgtacactc tctcctttaa ctttcttatc aaagcgattt
      241 cctttgatta atacatcagt gaatcctgta aaacgtacac ctgcatacat catgttatca
      301 aaatgattat ttaaaatttt aatattagag gggttctgtg tcgacaatgt ttgatagtgt
      361 gtgccaattg ctgttactaa ttccccagat ttatcacttt tgccaaaata ggaattttga
      421 atagtcacat tttcagattt tttcccatca tcattcaagg cataaggaaa accttttcta
      481 gttaatggtt caatctgaat gctctcctta cttatgattt gcccatcctt catcgttttg
      541 ggtaaggctt gcccaagaaa acgagaatta tcaactaata catttttcga acctgcaatt
      601 tgaatagcat gcccttgata actatccttg aatgttacat tttttatagt tacgttattt
      661 gaattcccaa tagcaaatgc acctgaagaa tttataagtg gtagattttt tgctttagtt
      721 ccttcttcat tcaaagcacc gttcatatca atcgtaccac cagaataact aatatcttct
      781 gttgggcccc attctacttg cgcaccatca tccgtaaata aacctgtcat aaaaacaatg
      841 gaagggtgat tcttaatatt tataccattt agaattgtag ctttctcatt caattctaag
      901 tgtgtatgac tttttaaaaa aacaatttct tttactaaat aagttccttc aggaaaatat
      961 acatttcctc cacctagccc ttgagctgca gcatctattg catcttgaat tgcttgtcga
     1021 tcatcatgaa tcccatcacc taccgcacca taatctttta cacttaaagc agtatcttca
     1081 aattcttttt tgataattct gtcacttata acatcttcct tataactctt attttcatta
     1141 gtagatgtta ctgtagattc aactgttctg ttgtctaatt gtgaattgga acgaatagta
     1201 ccattagagc tgatactatt aacacttaac gaattatcta aaccattagt ggttgaatta
     1261 gaggcaatca tttcagcaga acgattctga ttaacagttg gtgaattatc taaatcatta
     1321 gtaattgcat tagaagactc ttttgtagcc atcggtgaag aactagtaac aacttcatcc
     1381 gataaaatac cttggcttcc caaaattgaa attgccaata gtaccgagca agtccccacc
     1441 tttaatttac gaatactgaa tacctcatta ggaacaaaat atttcatcag aacctccaat
     1501 ttttttacta tttttaacaa ttgattatta cttttcttaa aatagataga atgacaccgc
     1561 tgtcattatc tttatcatat attttccaaa ttgaattgtc aaggaataac acaagaaaat
     1621 attcaaattt atactcttcg gaaatctctt caaactaggt cagtgccgcc ttgccctaag
     1681 tctaggttac tgacttgatc agttctatag tagactgaaa ctaaaatagt acacctctac
     1741 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatt ggtttgtcct attattattt
     1801 cattttacta tatctacaac ctcaaagcag ggttttgagc tatctatggc caacttccta
     1861 gtttgctctt tagccttcca ttcaaaagtg atgctatctt tcttagcttg aatataaaat
     1921 atcccattta ctatatttaa atttgttgat atgttgtaaa ttataaatga aagaacaaat
     1981 accttgtcat acaagcattc gccagggata tttacatgaa tatcgctcat atggcggatt
     2041 gaaactaaaa cactacaata tatttcttaa atagaccttc tataaaagct agatttaacc
     2101 tcgataaaaa tagatgcagt gcttctaaaa tactggaata gggcatatag gtaatcgtag
     2161 acaagtgttt tatagattgt ctcgcgggag atctaatgat gtatagcaag catcactaaa
     2221 cgtatagaat atttgtatta ttataatatc gatatgctat aaattctatc atgcaaaaga
     2281 atgactagaa catgaaagac aggcacaaaa ctttgaacaa tattctgaaa acaaaaaaag
     2341 acgcgagttt ccacaatctc actcatggaa actcacgtct ttcaaaaaat agggtgaatt
     2401 gaaaactaga acaagacact agattttcta aatgatgtct agtaaggatt ctaaatgcta
     2461 caccaaattg ctcacatctt atttcaaacc acgggactcc cacaagaaag gaaaatccta
     2521 taaaaattct ctctctgagg agaaaggagc ctcaactaca ggtgtaaact attatcaatt
     2581 gaaaacaaca gtttttacac tcaattagac tttttaagga caggatcact ttcgcttgac
     2641 tgtagtttgt catttttttg ataaagttag cactttttca gaaactgttt tgaaagtctc
     2701 aatgatatag tctacttctt catcgcttaa tttagtatga agagggaggg taatttcatt
     2761 ctcaaagaag gcataggcct taggatagtt cgtcatatca aatccaagat tcttataggc
     2821 tgtcaagaga ggaagcggtt tgtagtgaac attacttgca attcctgctt tagccaattc
     2881 ttggatgatg aggttgcgtt cttctaggct tgctccttct acacgggtga tgtagaggtg
     2941 gcgtgaagat tcgacagttt cagtcttgtg tgccaaagga tggatgcgag aacctgcaaa
     3001 accactatca tagcggtcca caatgtcctt acggcgttgt aacaaacttg gatagcggtc
     3061 caattgtacc aaaccaagtg aagccatgat atcggtcatg ttgcacttat aggctggtgt
     3121 aacgatatcg tattcccatg accccagttg catcttggca agagcatcct tagtttgccc
     3181 gtgaagggaa aggatttgga attccttgta catctcttcg tcatcaatca ctggattggc
     3241 tttccaagtc gcacttccac cttctgccgt tgtaaagttc ttaacggcat ggaatgagaa
     3301 ggaagtaaag tcagcgatag aaccagaagg ttgtccttta taagtagatc ccaaagcgtg
     3361 ggcactatca gagacaatga caatacggtt aaaggccttt tgccacttgc ttgaagcggt
     3421 aaagaagtca cgttttttct ccacgacttg gaacaaacga tcataatcgc aaacaatccc
     3481 tgcgagctct actgggataa tcaccttagt tttctcagtg atagcttgct caagcaggtc
     3541 atagtccatc tcaaacgtat ctgcttggat atccaccatg acaggggttg ctcccacgtg
     3601 cgtaatgaca ctacatgaag ccgtataggt catggctgga acgatgactt catcaccagg
     3661 tcccacttcc aaaacgcgta aaatcaactc cagagcggct gtcgcagagt tgagacaaac
     3721 agtcttaggt gtctgtgtgt aaagagacaa gcggcgctcc agttcttttg ttttaggacc
     3781 tgttgtgatc caaccagaac gcagggtatc cgctacttca gcaatttctg cttctgtgat
     3841 atcaggcggt gaaaatggaa tattgtaatt tggcattgat ttgctccttt tatctatact
     3901 cattttctta tgactacttt attttagtag ctcaaatacg gtttgaaaca tgattttgat
     3961 gtccccaaag aaactaaact ctcggagata ggcgaggtta tagcgcatct tttcaggaag
     4021 aacatgctcc acataggcct gatcaactga cagacctttc tccgtcattt gactgatgat
     4081 ggtgtcctca tccttgtagt tgatgctggc tggagaggta attcctgctt gcaagagcaa
     4141 ggttgccatc atttcagggc tatactgctc tgtataacgt ggcacttcag gtcgtgtacc
     4201 gacaaaggac atctcacctt taaggacatt gaccaactga ggcagttcgt ccaaacggac
     4261 acgtcggatg aaatttccaa ccttggtaat gcggctatcg ttagcagaag tcaccagact
     4321 tcctttttta tccgcatccg tcaccatggt acggaacttc caaatcttga accgacggtt
     4381 gtactgggtc acacgctctt gcttgtaaat cactggcccc ttgctatcca acttgatcca
     4441 aatgctcaag atgagaaaga tgggagaggt tagaaccagt aagaccaagg ccaaaaccca
     4501 gtccaagcaa cgcttgaaaa tcagcgaacc cttcctttta gagacaagct ggtagtaaga
     4561 ctcaacctcg cttgatttca tttccacagg caagtcttcc catttcagca tgctgttctc
     4621 ctttgttttt attatactat ttgtcaacat ttttcattat accacaaaat ggaaaaggcg
     4681 gtgaaagaaa tctgtaattt gaaggatttc cttctagtac tcaatgaaaa tcaaagtaca
     4741 gctttgagat tgcagataaa gctgacatgg tttaaagaga ttttcaaaga gtataggcca
     4801 tggcccctgc ctgcaaacta tacatcttgt gataggttcc tcccagagcc aagagttcct
     4861 catgggttcc actctcgata atgcgtccct tatccaagac atagatgcag ttggcgtctt
     4921 gaatagtaga aaggcggtga gcgatagcaa tagttgttcg gccctgtctc atcttcgcca
     4981 gagaagcttg aaccaagctt tctgtttcag agtcaatatt ggctgtcgct tcatccaaaa
     5041 tcaggatttt aggctggctg gcgactgttc tagcaaaggc aagaagctgg cgctgcccag
     5101 tagagaagct cgaaccacgc tcggaaacag gggagtcgta cccctgagga agttcttgaa
     5161 taaaggaatc tgcatccaca aaggctgccg cagcctgaac ctgctcatca ctgatttctt
     5221 ggtacatggc gatattggac ttaatagttc catgatagag gaagggttcc tgtaaaacca
     5281 aaccgatgtt ttttctcagc tcttcttgac tgaaatccct gatatccaca tcatccaaga
     5341 gaactctccc tgactggaat tcataaaagc gcatgaggac attgataata gacgatttcc
     5401 ctgaacctgt atgacctaca aaggcaatgg tttcaccctt attaacagag aaagaaatgt
     5461 catccagaat cggatgttta ccgtcatatg agaaacacac atgttcaaaa cggatattgc
     5521 cttcttggac tttggcttgc ccattttctt gaagaggttc ataggtcctc tcatcgatta
     5581 aggcaaagac acgacctgca gaaaccatag acgtttgcag agttgaaaag ttttgtgtca
     5641 cctcaatcaa ggggtcaaaa aggcggttga tgtactggat aaaggcatac atggtcccga
     5701 ccgttatccc gatagaaaga ccacgatagc caaaataggc catcaagaca gcataaccta
     5761 gaagtttcaa caaactcatg gcaggtctca aaaagagggc atccaaggct acagaacggt
     5821 tggcatagac caagtgctct tggttgattt catcaaattc tgcctgcagg cgcttctctt
     5881 gattaaaggc ctggattatt ctgattccct cgatgttttc agccagcttg ctattgatat
     5941 ctgacaagag acttctggtt ttctcaatga ttttcactga ttttttccga tagagattga
     6001 ccaaaaggaa aatcaagggg agaaagagca aaactaatgc tgtcaaacga aaatccaata
     6061 ccaacatggt ataaagggtt gtcagaaaga tgaaaactgc tgagataaag ctagataaaa
     6121 tccccgaaaa catatcactg atggtctcgg tgtcatttgt caaacgagag acgatagagc
     6181 ctgctggcgt cttgtcaaaa taagacatgc ccaacttttc catattggca aaggcatccc
     6241 gacgaatatc cctaacaata ctgtaagaca cgcgcgcaaa gagaagattg ccgacatact
     6301 gaactacagt ttgtaggatg tagagaccat agtagaccag caaaacggta acggctagtt
     6361 ggttaagatt gctgagatac tggtcgataa agtgggaagc cacgaggggt atgacacttt
     6421 taatgaccgt cgtcgctagg agaaaactga gtgccaaaaa ggtcaggagt ctataaggct
     6481 tgagataaga catcaagcgc ttcaatacag cccattgttc ttgtttattc tgcatcttct
     6541 tctcctttca tttctaactg ctgagactgg taggtttggg cataccagcc atccatcgct
     6601 agcaagtctt cgtgcgtgcc tcgttcgata atttgaccat tttgtagaac taaaataaaa
     6661 tctgcatgga caacagcact gaggcgatgg gcagtgataa tggttgtctt gtcctttcgc
     6721 gtctccttga ggttgtcgat aatcgcatac tctgtcttgg catctacggc ggataaggaa
     6781 tcatccaaaa tcaagatatc agggtctaaa atcatagccc gactcatagc caaccgttgc
     6841 ttttgaccac cagaaagact gactcctttt tcaccaatca gcgtatcaaa tccttgaggc
     6901 atgtctacaa tatcttggta aactcgggct agcttagtag cttcctcgac cgctgaaagg
     6961 ggcaagttag gattgccaaa gcggatattg tctaggattg aagtcgcaaa aagaaactgg
     7021 tcctgaggaa catagcccat gagactgcga aggtctgtca gacgatagtc ccgaatatcg
     7081 tgaccgttta gataaatggc acccttatcc acatcgtatt cacgcaagaa gagcttgatt
     7141 aaggacgttt tcccagagcc tgtctgccca accaagccca gtgtttgccc ttttgccaaa
     7201 ctaaagtgaa tatccgtcag tgtttcctca ttttcaaaag caaagctgtc aatggcatac
     7261 tccaaacgcc cattttcaat accatccaga ggaaactcag ggtcttgtac aggagattcc
     7321 tgagacaaaa gattttcaat ccgctggtaa gaaaccttcc ctcgctgagt agtattaaag
     7381 aggaagccga tggccataag aggccagacc agcatatcca aatagctgat aaaggtgact
     7441 agattcccaa ctgtaatctg cccttcctga accatcaagg agccaaccaa aagcgttaaa
     7501 acataggacg aaccaacaaa caagagaacc atagggtcaa agagactatc atatttcatg
     7561 gtttgcaggt tcttttggaa ggttaattca ttgactgcct gaaaagactt caactcgtct
     7621 gcctgataac cgaaagactt ggtcactttg atacctgata cggactcctg taccttgtta
     7681 ttgagttcag aaaaagcagc ttgggattcg ccaaaggcct tatgagtctt tctccctagg
     7741 cgactagtcg cataggccat gaaaggtagg gggagaatgg caacaagagt catctgccat
     7801 gagatgctaa agagcatggt caacaaagtc accagagccg tgatagaggc atccaccgca
     7861 gacatgacac cgccacctgc taaacgagtc aaggcattga tatcattggt tgcgtgtgcc
     7921 atcagatcac ccgtccgata ggtttgataa aaggctgacg acatttttgt gaaatgctta
     7981 aacaagcgag accgcatgat ctgtcccaag caataagagg tcccaaggat atacatacgc
     8041 cacacatagc gcaaatagta cataccaaag gctgcaagta gcaagtaaaa taggctaaga
     8101 aggaggtcct gctgggttaa ttgccccgat gtgatggcat caataacccg ccccataacc
     8161 ataggaggaa tgagattgag gacggaaacc aagaccaggg ccacaattcc gactagataa
     8221 cggcgttttt ctaacttgaa aaaccaccaa agtttttgaa taatggacat aaaatccctt
     8281 tctgattgca aatggaaacc tgaggccaat actcaatgaa aatcaaagag caaactagga
     8341 aactagccgc aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca
     8401 gtaacctaca tacggcaagg cgacgttgac gcggtttgaa gagattttcg aagagtacaa
     8461 gaccccaggt ttttcttatt cataagttac gactgtaaca gcacccttgt catattcagc
     8521 aataaagata ttggctacat tgtcatgccc ttgtttactg aggttatcaa gcaaccactc
     8581 ttcgctacga ccaatcgatt ccaagacatc tacttgaatc acaccgtcag tcacaactgg
     8641 atacttagga ttttcatctc ccatttgcac aacgatgagt tgcccatttt gctcttgcac
     8701 agctcgtttg acttgcttca tctggaaaat cccttggcta cgaagtttga gggctacatc
     8761 cgatgcagac aaaccaactg aacgacaggt ttatgggtca atattcccat ttttgataag
     8821 gagagttggc ttaccatcaa tcaaacgttt cacaaagtga acattgttgt tgagccattt
     8881 gagagtcaag accagtttga ttaaaatttc gatataatta agtgtcattt gttcacatcc
     8941 accagttcaa ttgcgtctgt cttatacaat tcgactttct ctaacaggta cttgtctggc
     9001 tcacttccgt ttagggcacg ataatagcga tctcccacct tgataagtgc gccatctgtg
     9061 tttgtggaag tattaacata gacttctgac ttgtctactc ccaaatcttt ggaaacaacc
     9121 tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa cttgattgtt ttgataagtc
     9181 gtgatgctga ccaaaagcag agctaaataa ggccagagct gaaatcatga ccaactcacg
     9241 aaattcggtt ccctttttat catggtatgc cttaaaagca aaaaaaaact gtcacagcta
     9301 atagaacaat cccaaagcca accatgatac cattttgctg accgatttgg ctgagtacat
     9361 agtcataaga gtagaatttc atgtattttc actccctttc ataaaatcat tcttaattat
     9421 aaacgaaaga gagtaatttt tcaaaccata cgttggg
//
LOCUS       DAVGJJ010000078         9289 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000078 DAVGJJ010000000
VERSION     DAVGJJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.078"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            236..925
                     /locus_tag="VVE35_001649"
     CDS             236..925
                     /locus_tag="VVE35_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3061802.1"
                     /translation="MKKYQLLFKTSAILSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            967..1215
                     /locus_tag="VVE35_001650"
     CDS             967..1215
                     /locus_tag="VVE35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061803.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLVSDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            1245..1856
                     /locus_tag="VVE35_001651"
     CDS             1245..1856
                     /locus_tag="VVE35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3061804.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGSLLLGFAYKKSQNLWVSTLVHMLYNSWPLLTYL"
     gene            2017..2811
                     /locus_tag="VVE35_001652"
     CDS             2017..2811
                     /locus_tag="VVE35_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU3061805.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            2808..3443
                     /gene="trmB"
                     /locus_tag="VVE35_001653"
     CDS             2808..3443
                     /gene="trmB"
                     /locus_tag="VVE35_001653"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU3061806.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3569..4048
                     /gene="rimP"
                     /locus_tag="VVE35_001654"
     CDS             3569..4048
                     /gene="rimP"
                     /locus_tag="VVE35_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU3061807.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4092..5228
                     /gene="nusA"
                     /locus_tag="VVE35_001655"
     CDS             4092..5228
                     /gene="nusA"
                     /locus_tag="VVE35_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU3061808.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5250..5543
                     /locus_tag="VVE35_001656"
     CDS             5250..5543
                     /locus_tag="VVE35_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU3061809.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5536..5835
                     /locus_tag="VVE35_001657"
     CDS             5536..5835
                     /locus_tag="VVE35_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU3061810.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            5852..8644
                     /gene="infB"
                     /locus_tag="VVE35_001658"
     CDS             5852..8644
                     /gene="infB"
                     /locus_tag="VVE35_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU3061811.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGAAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            8895..9245
                     /gene="rbfA"
                     /locus_tag="VVE35_001659"
     CDS             8895..9245
                     /gene="rbfA"
                     /locus_tag="VVE35_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU3061812.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2819 a         1749 c         2164 g         2557 t
ORIGIN      
        1 attatggata ctgaaatgct tgcttgtata gagggtgggg atgtatctgg tatttataga
       61 gggtatgctt atcaggacag tccatttggt ccttaccctt ctatattaaa aaattcaggt
      121 cctttcccag taagtgggta ctgcccgcgt ggttatcgtg accttggtta tataggagca
      181 ggttttcatt tatgtggaat ataggctgaa atataagaaa tgaggaagaa tttctatgaa
      241 aaagtatcag cttctattca aaacaagtgc aattttatcc tacctatttt tcgtatttgg
      301 tctttctcag ctgacgctta tcgtccaaaa ttattggcaa ttttcttccc agattggtaa
      361 tttcgtctgg attcaaaata tcttgagttt actttttatt ggagtcatga ttgtggttct
      421 tgttaagaca ggccatggtt atctcttccg cattccaaga aaaaaatggc tttggtattc
      481 gattttgaca gtattagtgg tagtgttcca gatctctttt aacgttcaga cagctaaaca
      541 tgttcagtca actgcggaag gttgggctgt attgattggt tatagtggga ctaactttgc
      601 agagctaggt atttatatag ccctgttctt tctggttcca ctgatggaag agttaatcta
      661 tagaggatta ctgcaacatg ctttctttaa gcattcgcga tttggtcttg atttgcttct
      721 tccttctatt ttatttgctc tccctcattt ttcaagcctg cctagtctgt tagatatctt
      781 cgtctttgca acagttggaa tcatctttgc tggtttgacc cgctatacca agagcattta
      841 tccatcctat gcggtgcatg tgatcaataa tattgtagcg accttcccgt ttttgctcac
      901 ttttctacat agggtcttgg ggtaaaaaaa caaaaggctt gcttttcagc catagaggag
      961 gtcatcatgt ataaacactt atttttccta gattccaaaa ctttagatcg gttgacaccc
     1021 tatattctag tcttggtttc tgacaccatt gcttttaatg tttttgtgct aacctttgta
     1081 tctgcggtgg tttttaattt cctaaattcc atgctagctt taatggctat attcataggg
     1141 gctggctatg tggtcggatt ttggttgcta aaatggtttg ttttggaaag gctagatcta
     1201 aagaatgacg tgtaggaagt ctgtttgttg aggaggataa ttttatggag ttttttgata
     1261 aatttcatgc cttgtgtttt ggatttttag tactaataat tgtcattaca gttccttata
     1321 cgattaacca tggggatttt tttcaaaatg aatctgcatt gattcttgta agtcttcttg
     1381 taacctcgct gagtgttgct tatgctagaa agtttgaaat gatttctttt gggatgttaa
     1441 gcaagaaaca acttttgctt ttcattgcaa tctttcttct aagtgtactt gagacgctgg
     1501 tttatattca tttcttcgct gtttcttctg gctcaggggt ccaacacttg gcggaagtca
     1561 gcagaggaat ttccctgtct ttgattttaa ctacctcagt ttttggcccc atccaggagg
     1621 aactcatttt cagaggactt cttcaaggtg cggtttttga caattcttgg ttagggcttg
     1681 tgctaacttc ctctctcttt tctttcatgc atggaccttc taatgtccct tcgtttattt
     1741 tttatctact tgggagtttg ttgctgggct ttgcttataa aaagagtcaa aacctatggg
     1801 tttctactct agtccatatg ctttacaaca gttggccact cttaacttat ttataaaaat
     1861 tatgaaaagg taagcagaag acggtgctta cctttttcaa gctataatga tacccaagcc
     1921 aatccagagg cttttctttg agaatcgggt tagagtggtt gacgaatagg ccaaaaacta
     1981 gtagaatagt aaggaaactt tatacggagg aaagaaatgg atttgggtga taatgagcta
     2041 acactgactc ccatacctgg gaaaagtggc aaggcttata tgggtagcta tcctgatggg
     2101 aagcgcatct ttgtaaaaat gaacacctct ccaatcctac ctggtctagc tagagaacaa
     2161 attgctccac aattattatg gagtcgtcgt ttggcagatg ggcgtgatat gtgtgctcaa
     2221 gaatggttga caggcaagat attgaccccc tatgatatga atcgtaagca aatcgtcaat
     2281 attttaaccc gtcttcatcg ctcacgtccg ttgatgacac aattgagtcg tttgggctat
     2341 gccatggaaa cacctgtaga tttactacag tcttggcagg aaacggctcc agatgctttg
     2401 cgtaaaaatc attttatcag tgaagtgatg gctgatttac gtcagactat tccaggattt
     2461 agagaggacc atgcgaccat tgtccatgga gatgtacgac atagtaattg gattgagaca
     2521 gatagtggct tgatttattt agtagattgg gattcggttc gcttgaccga tcgcatgttt
     2581 gatgtggccc atatgctctg ccattatatt tcagaacatc agtggaagga atggttgacc
     2641 tactacggtt acaagtacaa tcaaacggta ttaagtaaat tgtattggta tggtcaattg
     2701 tcttatttga gtcagatttc caagtattat atgaaccaag atttagaaaa tgtcaatcgg
     2761 gagattcatg gtttgcgtca tttccgagac aagtatggaa agagaagatg agagttagaa
     2821 atcgtaaagg ggcaacagaa ttactagagg caaatcccca gtatgtggtc ctcaatccct
     2881 tggaagccaa ggcaaaatgg cgggacttgt ttggcaatga taatcccatt catgtggaag
     2941 ttggaagtgg aaagggtgcc tttgtttcag gtatggccaa gcaaaaccct gacatcaact
     3001 atatcgggat tgatattcaa aagtctgttt tgagctacgc tttggacaag gtgcttgaag
     3061 ctggagtgtc taacatcaag ctcttgtggg tagatggttc tgacttgact gactactttg
     3121 aagacggtga gattgatcgc ttgtatctga acttttcaga tccatggccg aaaaaacgcc
     3181 atgaaaagcg tcgtttgacc tacaagacct tcttggatac cttcaaacgt atcttgcctg
     3241 aaaatggaga aattcatttc aagacggata accgtggctt gtttgagtac agtttagtga
     3301 gcttttctca atatggcatg aaactcaatg gtgtctggtt agatttgcat gccagtgatt
     3361 ttgaaggcaa tgtcatgaca gaatacgagc aaaaattctc aaacaagggg caagttatct
     3421 accgagttga ggcagaattt taagagataa cctaaaatta ggctgtacaa gtgcttttgc
     3481 tttacataag ttggcaaacg tgctatactg atagtaagaa tatgaaaagt gaggcgggga
     3541 aatatcttcg cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg tagaattagt
     3601 cagagaagtt gtagaacctg tcatagaagc tccttttgaa ctcgtggata tcgagtatgg
     3661 aaagattggc agtgacatga ttctcagtat ttttgtagat aaacccgaag gaattacctt
     3721 gaacgacacg gcagacttga cagaaatgat tagtcctgtc ctagacacca tcaagccaga
     3781 tcccttccca gaacaatatt tcctagaaat taccagtcca ggtttggaac gtcctttgaa
     3841 aaccaaggat gccgtcgctg gagcggttgg aaaatacatc catgtcgggc tctaccaagc
     3901 catcgataag caaaaggtct ttgaaggaac cttgttggcc ttcgaagagg acgagttgac
     3961 tatggaatat atggacaaga cgcgtaagaa aaccgtccaa attccataca gtttagtatc
     4021 aaaagcacgt ttagcagtta aattatagaa aaagaaagga tagcttttga ggattcaaaa
     4081 gtgaagaaaa catgagtaaa gaaatgctag aggccttccg cattttggaa gaagacaagg
     4141 gaatcaaaaa agaagatatc atcgacgcag tagtagagtc gcttcgttcc gcttatcgca
     4201 gacgctatgg tcagtcagac agcgtagcta ttgacttcaa cgaaaaaaca ggtgacttta
     4261 cagtttatac tgtccgtgaa gttgttgatg aagtatttga tagccgtttg gaaatcagct
     4321 tgaaagatgc tcttgccatt aattcagctt atgaacttgg agacaaaatc aagtttgaag
     4381 aagcaccagc tgagtttggt cgtgtagcag cccaatctgc caaacaaacc atcatggaaa
     4441 aaatgcgcaa gcaaacacgt gccatcactt acaatactta caaagaacat gagcaagaaa
     4501 tcatgtctgg tacagtagaa cgctttgaca accgctttat ctatgtcaac cttggtagca
     4561 tcgaagccca attgtcaaaa caagaccaaa ttcctggaga agtttttgct tctcatgatc
     4621 gtatcgaagt ttatgtttac aaggttgaag acaaccctcg tggtgtgaac gtctttgtta
     4681 gccgtagcca tccagaaatg atcaaacgtt tgatggagca agaaattcca gaagtttatg
     4741 atggaactgt tgaaatcatg agcgtggctc gtgaagcagg tgaccgtacg aaggttgctg
     4801 ttcgtagcca caatccaaac gtggatgcta tcggtacaat cgttggacgt ggtggtgcta
     4861 atatcaagaa gattactagc aaattccacc cagctcgtta cgatgctaaa aatgaccgca
     4921 tggtaccaat cgaagaaaat atcgatgtta tcgagtgggt agcagatcca gctgaattta
     4981 tctacaatgc catcgctcct gctgaggttg accaagttat ctttgatgaa aacgacagca
     5041 aacgtgcctt ggtggttgtt ccagataaca agctttctct tgccattggt cgtcgtggac
     5101 aaaacgtgcg cttggcggct cacttgactg gttaccgtat cgatatcaag tctgctagcg
     5161 aatttgaagc catggaagac gctgcttcag tagagttgga agtagaaaac gatactgtag
     5221 aagaataaaa gctgctagag gagggaaaga tgaaaacaag aaaaatccct ttgcgcaagt
     5281 ctgttgtgtc taacgaagtg attgataagc gtgatttgct ccgcattgtc aagaacaagg
     5341 aaggacaagt ctttattgat cctacgggca aggccaatgg ccgcggcgct tatatcaaac
     5401 tagacaatgc agaagcccta gaggcgaaaa agaagaaggt ctttaaccgc agctttagca
     5461 tggaagtgga agaaagcttt tatgacgagt tgatcgctta tgtggatcac aaagtgaaaa
     5521 gaagagagtt gggacttgaa taagcaaaag ataagtaatc tcttggggct tgctcagcga
     5581 gcagggcgca tcatatcggg tgaagaattg gtggtcaagg ccattcaaga cggcaaggcc
     5641 aagttggtct ttctagctca tgatgctgga cccaatctga ccaagaagat tcaagataaa
     5701 agtcattatt atcaagtaga aattgtaacc gtgttttcaa cactggaatt aagcatagca
     5761 gtcgggaaat cgagaaaggt tttggctgta acagatgctg gatttacaaa gaaaatgagg
     5821 tctcttatgg aatagaagag gaggacatga tttgtctaag aaaagattgt acgaaatcgc
     5881 aaaagaactt ggaaaagaaa gtaaagaagt tgtagcgcgt gcaaaagagt tgggcttgga
     5941 tgtgaaaagc cactcatcaa gtgtggaaga agctgtcgct gcaaaaattg ctgccagctt
     6001 taagcctgca gctgctccga aagtagaagc aaaacctgca gcaccaaaag taagtgcaga
     6061 aaagaaagcc gaaaaatctg agccagctaa accagctgta gctaaggaag aggcaaaacc
     6121 ggctgagcca gttgctccga aaacagaaaa agtagcagcg aaaccgcaaa gccgtaattt
     6181 caaggctgag cgtgaagccc gtgctaaaga gcaggcagaa cgacgtaagc aaaataaggg
     6241 caataaccgt gaccaacaac aaaacggaaa ccgtcagaaa aacgacggac gtaatggtgg
     6301 aaaacaaggt caaagcaacc gcgacaatcg tcgctttaat gaccaagcta agaagcagca
     6361 aggtcagcaa aaacgtagaa atgagcgccg tcagcaagag gacaaacgtt caaatcaagt
     6421 ggctccacgt attgacttta aagcccgtgc agcagcccta aaagcagagc aaaatgcaga
     6481 gtacgctcgt tcaagtgagg aacgcttcaa gcagtatcag gctgctaaag aagccttggc
     6541 tcaagctaac aaacgcaagg aaccagagga aatctttgaa gaagcggcta agttagctga
     6601 acaagcacag caagttcaag cagtggttga agtcgtccct gagaaaaaag aacctgcagt
     6661 ggatacacgt cgtaaaaaac aagctcgacc agacaaaaat cgtgacgatt atgatcatga
     6721 agaagatggt cctagaaaac aacaaaagaa tcgaagtagt caaaatcaag tgagaaatca
     6781 aaagaatagt aactggaata acaacaaaaa gaacaaaaaa ggcaataaca agaacaaccg
     6841 taatcagact ccaaaacctg ttacggagcg taaattccat gaattgccaa cagaatttga
     6901 atatacagat ggtatgaccg ttgcggaaat cgcaaaacgt atcaaacgtg aaccagctga
     6961 aattgttaag aaacttttca tgatgggtgt catggccaca caaaaccaat ccttggatgg
     7021 ggaaacaatt gaactcctca tggtggatta cggtatcgaa gccaaacaaa aggttgaagt
     7081 ggataatgct gacatcgaac gtttctttgt cgaagatggt tatctcaatg aagatgaatt
     7141 ggttgagcgt ccaccagttg ttactatcat gggacacgtt gaccacggta aaacaaccct
     7201 tttggatact cttcgtaact cacgtgttgc gacaggtgaa gcaggtggta ttactcagca
     7261 tatcggtgcc taccaaatcg tggaaaatgg taagaagatt accttccttg atacaccagg
     7321 acacgcggcc tttacatcaa tgcgtgcgcg tggtgcttct gttaccgata ttacgatctt
     7381 ggtcgtagcg gcagatgacg gggttatgcc tcagactatt gaagccatca accactcaaa
     7441 agcagctaac gttccaatca tcgtagctat taacaagatt gataaaccag gtgctaaccc
     7501 agaacgcgtt atcggtgaat tggcagagca tggtgtgatg tcaaccgctt ggggtggaga
     7561 ttctgaattt gttgaaatct cggctaaatt caaccaaaat atcgaagaat tgttggaaac
     7621 agtccttctt gtggctgaaa tccaagaact caaagcagac ccaacagttc gtgcgatcgg
     7681 tacggttatc gaagcgcgct tggataaagg aaaaggtgcg gtcgcaaccc ttcttgtaca
     7741 acaaggtacc ttgaatgttc aagacccaat cgttgtcgga aataccttcg gtcgtgtccg
     7801 tgctatgacc aacgaccttg gtcgtcgtgt taaagttgct ggaccatcaa caccagtctc
     7861 tatcacaggt ttgaacgaag caccgatggc gggtgaccac tttgccgttt acgaggatga
     7921 aaaatctgcg cgtgcagcag gtgaagagcg tgccaaacgt gccctcatga aacaacgtca
     7981 agctacccaa cgtgttagcc ttgaaaacct ctttgatacc cttaaagctg gggaactcaa
     8041 atctgttaat gttatcatca aggccgatgt acaaggttct gttgaagccc tttctgcctc
     8101 acttcaaaag attgacgtgg aaggtgtcaa agtgactatc gtccactcag cggtcggtgc
     8161 tatcaacgaa tcagatgtga cccttgccga agcttcaaat gcctttatcg ttggtttcaa
     8221 cgtacgccct acaccacaag ctcgtcaaca agcagaagct gacgatgtgg aaatccgtct
     8281 tcacagcatt atctacaagg ttatcgaaga gatggaagaa gctatgaaag ggatgcttga
     8341 tccagaattt gaagaaaaag ttattggtgc agcggttatc cgtgaaacct tcaaggtgtc
     8401 taaagtcgga actatcggtg gatttatggt tatcaacggt aaggttgccc gtgactctaa
     8461 agtccgtgtt atccgtgatg gtgtcgttat ctatgatggc gaactcgcaa gcttgaaaca
     8521 ctacaaagat gacgtgaaag aagtgacaaa cggtcgtgaa ggtggattga tgatcgacgg
     8581 ctacaatgat attaagatgg atgatgtgat tgaggcgtat gtcatggaag aaatcaagag
     8641 ataagatttt ttgctccttt cttaggtggt gagggacgca agcaaaccga tggtttcatt
     8701 gcttattttt gagcctaggg tctcaaaaat cccctgtgat gggactgata aatcagttcc
     8761 atcactttca ccacggcgaa agaagcagat gacttcaaat tgaacttcgt ttcaatttaa
     8821 actgaaaatc aagaagttta aaatagctag gtctgctggc ctagcttttg gttcaaagta
     8881 gagaaaggaa tatcatggca aatcatttcc gtacagatcg tgtgggcatg gaaatcaagc
     8941 gtgaagtcaa tgagattttg caaaagaaag tccgtgatcc acgtgtccaa ggtgtgacca
     9001 tcacagatgt tcagatgctg ggtgacttgt ctgttgccaa ggtttattac accattttga
     9061 gtaaccttgc ttcggataac caaaaagccc aaatcgggct tgaaaaagca actggtacca
     9121 tcaaacgtga acttggtcgc aatttgaaat tgtacaaaat cccagatttg accttcgtca
     9181 aagacgagtc catcgagtat ggaaacaaga ttgacgagat gctacgcaat ctggataaga
     9241 actaaagaag aggggttgcc cctctttttt ggtggaggaa aataggttg
//
LOCUS       DAVGJJ010000079         8761 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000079 DAVGJJ010000000
VERSION     DAVGJJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.079"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            13..1989
                     /locus_tag="VVE35_001660"
     CDS             13..1989
                     /locus_tag="VVE35_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU3061813.1"
                     /translation="MKKVRFIFLALLFFLASPEGAMASDGTWQGKQYLKEDGSQAANE
                     WVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGYMAKSEWVEDKGVFYYLDQD
                     GKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKADGQHAEKEWLQIKGKDYYF
                     KSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFYIKENGNYADKEWIFENGHY
                     YYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEW
                     IWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKFGGYMTANEWIWDKESWFYLKSDG
                     KMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVV
                     PVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDAS
                     KDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLF
                     KDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESN
                     WGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGN
                     KASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(2050..3705)
                     /gene="pavA"
                     /locus_tag="VVE35_001661"
     CDS             complement(2050..3705)
                     /gene="pavA"
                     /locus_tag="VVE35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU3061814.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            3810..4307
                     /gene="ybeY"
                     /locus_tag="VVE35_001662"
     CDS             3810..4307
                     /gene="ybeY"
                     /locus_tag="VVE35_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU3061815.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            4288..4683
                     /locus_tag="VVE35_001663"
     CDS             4288..4683
                     /locus_tag="VVE35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU3061816.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            4700..5599
                     /gene="era"
                     /locus_tag="VVE35_001664"
     CDS             4700..5599
                     /gene="era"
                     /locus_tag="VVE35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU3061817.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            5647..6471
                     /gene="mutM"
                     /locus_tag="VVE35_001665"
     CDS             5647..6471
                     /gene="mutM"
                     /locus_tag="VVE35_001665"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU3061818.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            6471..7076
                     /locus_tag="VVE35_001666"
     CDS             6471..7076
                     /locus_tag="VVE35_001666"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU3061819.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            7063..8262
                     /locus_tag="VVE35_001667"
     CDS             7063..8262
                     /locus_tag="VVE35_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU3061820.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMIIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKKKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            8262..8411
                     /gene="rpmG"
                     /locus_tag="VVE35_001668"
     CDS             8262..8411
                     /gene="rpmG"
                     /locus_tag="VVE35_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3061821.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            8451..8684
                     /gene="secG"
                     /locus_tag="VVE35_001669"
     CDS             8451..8684
                     /gene="secG"
                     /locus_tag="VVE35_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU3061822.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
BASE COUNT         2481 a         1605 c         2016 g         2659 t
ORIGIN      
        1 ggaagaaggt taatgaagaa agtaagattt atttttttag ctctgctatt tttcttagct
       61 agtccagagg gtgcaatggc tagtgatggt acttggcaag gaaaacagta tctgaaagaa
      121 gatggcagtc aagcagcgaa tgagtgggtt tttgatactc attatcaatc ttggttctat
      181 ataaaagcag atgctaacta tgctgataat gaatggctaa agcaaggtga cgactatttt
      241 tacctcaaat ctggtggcta tatggccaaa tcagaatggg tagaagacaa gggagtcttt
      301 tattatcttg accaagatgg aaagatgaaa agaaatgctt gggtaggaac ttcctatgtt
      361 ggtgcaacag gtgccaaagt aatagaagac tgggtctatg attctcaata cgatgcttgg
      421 ttttatatca aagcagatgg acagcacgca gagaaagaat ggctccaaat taaagggaag
      481 gactattatt tcaaatccgg tggttatcta ctgacaagtc agtggattaa tcaagcttat
      541 gtgaatgcta gtggtgccaa agtgcagcaa ggttggcttt ttgacaaaca ataccaatct
      601 tggttttaca tcaaagaaaa tggaaactat gctgataaag aatggatttt cgagaatggt
      661 cactattatt atctaaaatc cggtggctac atggcagcca atgaatggat ttgggataag
      721 gaatcttggt tttatctcaa atttgatggg aaaatagctg aaaaagaatg ggtctacgat
      781 tctcatagtc aagcctggta ctacttcaaa tccggtggtt acatgacagc caatgaatgg
      841 atttgggata aggaatcttg gttttacctc aaatctgatg ggaaaatagc tgaaaaagaa
      901 tgggtctacg attctcatag tcaagcttgg tactacttca aattcggtgg ttacatgaca
      961 gccaatgaat ggatttggga taaggaatct tggttttacc tcaagtctga tggcaaaatg
     1021 gctgaaaaag aatgggtcta cgattctcat agtcaagctt ggtactactt caaatctggt
     1081 ggctacatgg cgaaaaatga gacagtagat ggttatcagc ttggaagcga tggtaaatgg
     1141 cttggaggaa aagctacaaa tgaaaatgct gcttactatc aagtagtgcc tgttacagcc
     1201 aatgtttatg attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg
     1261 ctagataagg atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca
     1321 ggctatatga aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat
     1381 tatgagagtg atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta
     1441 gcttctcatc tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgcat
     1501 tttgatggtt ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac
     1561 tacagtgctg aagaattgga taaggtattt agtttgctaa acattaacaa tagccttttg
     1621 gagaacaagg gcgctacttt taaggaagcc gaagaacatt accatatcaa tgctctttat
     1681 ctccttgccc atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag
     1741 aataatttct ttggcattac agcctatgat acgacccctt acctttctgc taagacattt
     1801 gatgatgtgg ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatcgat
     1861 aggggaagaa ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac
     1921 ccttattggg gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc
     1981 aaagattagt actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt
     2041 caagtaaaat caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag
     2101 ggtcttttgt cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt
     2161 tttgacttca atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc
     2221 agctaactct gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac
     2281 aacatggctt ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt
     2341 aaaggtcaat tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc
     2401 gcttgctaga tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg
     2461 gataaaacct gtttgaatca attcttcgcg gatttcagcg atttcttcca gtccagcttg
     2521 gttgaggacg gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa
     2581 atcagtcaag tatttgacag cttctttgag tttctgatac cgtttaaaat agcgttgggc
     2641 attctggttg ggagtcagag ccttatcaag cgcaatcatg ataggttggt tggtatagta
     2701 gttgtctagg ataacctggt cttggtcgtt aggcacttgg tgtaggaagg ttgtcagcaa
     2761 ttctcctttt tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt
     2821 tttgagtttg tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc
     2881 ctgctgtttg acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga
     2941 aagatttgca aaaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc
     3001 agtcaagcat ggcttggttt cttgattgaa aaaatttcgg aaagcggaaa gtttttcact
     3061 aaccagtatc ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg
     3121 aagatttttt gctgttagtt cttgggtttg caggatttca aagagctttt catctttgat
     3181 agtaaaagga ttgagagatt ctgtacttgg cggagcgata taggtcgatc ctggaagtaa
     3241 ggtgcggtag ctattttgtg aaaaaccgac gtgtttgata acttcgagga ttttatgact
     3301 gcttttatcg accagtagaa tattactgtg tttccccata atttcaataa tcaaggtagc
     3361 ctggatatgg tctccaatct cgtttttatt ggaaactgta atttccacaa tacggtcatt
     3421 ttccacttgc tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat
     3481 aaaggtagaa ggttgagctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa
     3541 aactggatgg gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac
     3601 caactcttgt tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg
     3661 caattcctca actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg
     3721 attgtattcc atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat
     3781 aaagccaagt aaagagaaac gagaagcaca tgtatattga aatggtagat gaaactggtc
     3841 aagtttcaaa agaaatgttg caacaaaccc aagaaatttt ggaatttgca gcccaaaaat
     3901 taggaaaaga agacaaggag atggcagtca cttttgtgac caatgagcgt agtcatgaac
     3961 ttaatctgga gtaccgtgac accgaccgtc cgacagatgt catcagcctt gagtataaac
     4021 cagaattgga aattgccttt gacgaagagg atttgcttga aaatccagaa ttggcagaga
     4081 tgatgtctga gtttgatgcc tatattgggg aattgttcat ctctatcgat aaggctcatg
     4141 agcaggccga agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg
     4201 gctttttaca tattaacggc tatgatcact atactccgga agaagaagcg gagatgttcg
     4261 gtttacaaga agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa
     4321 aatcgtgact tgatatccag tttagaattt gccatcacag ggatttttac tgctatcaag
     4381 gaagaacgaa atatgcgaaa acatgcagtg acggctctag tggtcatcct tgcaggtttt
     4441 gtttttcagg tgtcacgaat cgaatggctc tttcttctat tgagtatttt cttggtagta
     4501 gcctttgaga ttatcaactc tgctattgaa aatgtggtgg atttggccag tcactatcac
     4561 ttttccatgc tggctaaaaa tgccaaggat atggcggccg gcgcggtatt agtggtttct
     4621 ctttttgcag ccttaacagg cgcattgatt tttctcccac gaatctggga tttattattt
     4681 taaacagtaa gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc
     4741 caatgttggg aagtcaacct ttttaaatca cgttatgggg caaaagattg ccattatgag
     4801 tgacaaggcg cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca
     4861 aattgtcttt atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat
     4921 ggttgagtct gcctacagta cccttcgcga agtggacacc gttcttttca tggtgcctgc
     4981 tgatgaagcg cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt
     5041 tcctgtgatt ttggtggtga ataaaatcga taaggtccat ccagaccagc tcttgtctca
     5101 gattgatgac ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca
     5161 gggaaataac gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca
     5221 atatttcccg tctgatcaaa tcacagacca tccagaacgt ttcttagttt cagaaatggt
     5281 tcgcgagaaa gtcttgcacc taactcgtga agagattccg cattctgtag cagtagttgt
     5341 tgactctatg aaacgagacg aagagacaga caaggttcac atccgtgcaa ccatcatggt
     5401 cgagcgcgat agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat
     5461 cggtagcatg gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac
     5521 ctgggtcaag gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta
     5581 taatgaaaaa gaatactaag tagaggtggg ctcatgcctg cttcttgttt tacagaagga
     5641 ggacttatgc ctgaattacc tgaggttgaa accgtttgtc gtggcttaga aaaattgatt
     5701 ataggaaaga agatttcgag tatagaaatt cgctacccca agatgattaa gacggatttg
     5761 gaagagtttc aaagggaatt gcctagtcag attatcgagt caatgggacg tcgtggaaaa
     5821 tatttgcttt tttatctgac agacaaggtc ttgatttccc atttgcggat ggagggcaag
     5881 tatttttact atccagacca aggacctgaa cgcaagcatg cccatgtttt ctttcatttt
     5941 gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat ggaactcttg
     6001 gtgcctgacc ttttagacgc ctactttatt tctaaaaaat taggtcctga accaagcgaa
     6061 caagactttg atttacaggt ctttcaatct gcccttgcca agtccaaaaa gcctatcaaa
     6121 tcccatctcc tagaccagac cttggtagct ggacttggca atatctatgt ggatgaggtt
     6181 ctctggcgag ctcaggttca tccagctaga tcttcccaga ctttgacagc agaagaagcg
     6241 acagctattc atgaccaaac cattgctgtt ttgagccagg ctgttgaaaa aggcggttcc
     6301 accattcgga cctataccaa tgcctttggg gaagatggaa gcatgcagga ttttcatcag
     6361 gtctatgata agactggtca agaatgtgta cgctgtggta ccatcattga gaaaattcaa
     6421 ctaggcggac gaggcaccca cttttgtcca aactgtcaaa ggagggactg atgagaaaaa
     6481 tcatcggaat cactggggga attgcctctg gtaagtcaac tgtgacaaat tttctaagac
     6541 agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca ccaactacag aaacctggtg
     6601 gtcgtctgtt tgaggctcta gtacagcact ttgggcaaga aatcattctt gaaaacggag
     6661 aactcaatcg ccctctccta gctagtctca tcttttcaaa tcctgaagag caaaaatggt
     6721 ctaagcaaat tcaaggggag attatccgtg aggaactggc tactttgaga gaacagttgg
     6781 ctcagacaga agagattttc ttcatggata ttcccctact ttttgagcag gactacagcg
     6841 attggtttgc tgagacttgg ttggtctatg tggaccgaga tacccaagta gaacgcttaa
     6901 tgaaaaggga ccagttgtcc aaggatgaag ctgagtctcg tctggcagcc cagtggcctt
     6961 tagaaaaaaa gaaagatttg gccagccagg ttcttgataa taatggcaat cagaaccagc
     7021 ttcttaatca agtgcatatc cttcttgagg gaggtaggca agatgacaga gattaactgg
     7081 aaggataatc tgcgcattgc ctggtttggt aattttctga caggagccag tatttctttg
     7141 gttgtacctt ttatgcccat cttcgtggaa aatctaggtg tagggagtga gcaagtcgct
     7201 ttttatgcag gcttagcaat ttctgtctct gctatttccg cggcgctctt ttctcctatt
     7261 tggggtattc ttgctgacaa atacggccga aaacccatga taattcgggc aggtcttgct
     7321 atgactatca ctatgggagg cttggccttt gtcccaaata tctattggtt aatctttctt
     7381 cgtttactaa acggtgtatt tgcaggtttt gttcctaatg caacggcact gatagccagt
     7441 caggttccaa agaagaaatc aggctctgcc ttaggtactt tgtctacagg cgtagttgca
     7501 ggcactctaa ctggtccctt tattggtggc tttatcgcag aattatttgg cattcgtaca
     7561 gttttcttac tggttggtag ttttctattt ttagctgcta ttttgactat ttgctttatc
     7621 aaggaagatt ttcaaccagt agccaaggaa aaggctattc caacaaagga attatttacc
     7681 tcggttaaat atccctatct tttgctcaat ctctttttaa ccagttttgt catccaattt
     7741 tcagctcaat cgattggccc tattttggct ctttatgtac gcgacttagg gcagacagag
     7801 aatcttcttt ttgtctctgg tttgattgtg tccagtatgg gcttttccag catgatgagt
     7861 gcaggagtca tgggcaagct aggtgacaag gtgggcaatc atcgtctctt ggttgtcgcc
     7921 cagttttatt cagtcatcat ctatctcctc tgtgccaatg cctctagccc ccttcaacta
     7981 ggactctatc gtttcctctt tggattggga accggtgcct tgattcccgg ggttaatgcc
     8041 ctactcagca aaatgactcc caaagccggc atttcgaggg tctttgcctt caatcaggta
     8101 ttcttttatc tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc aggtcaattt
     8161 ggctaccatg ctgtctttta tgcgacgagc ctttgtgttg cctttagttg tctctttaac
     8221 ctgattcaat ttcgaacatt attaaaagta aaggaaatct agtgcgagta aaaattaatc
     8281 tcaaatgctc ctcttgtggc agtatcaatt acctaaccag taaaaattca aaaacccatc
     8341 cagacaagat tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc ctacatcttg
     8401 aatctaagta gattttatgg taaaataata gggattttaa ggagtttgat atgtataacc
     8461 tattattaac cattttatta gtattatctg ttgtgattgt gattgcaatt ttcatgcaac
     8521 caaccaaaaa ccaatccagc aatgtatttg atgccagttc aggtgatttg tttgaacgca
     8581 gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac agggatttta gtctttttct
     8641 ggctagccat tgccttagca ttgacggtat tatcaagtag ataagaaaat aatgggcagg
     8701 actaggtctt tgcctctttt tatttttaaa ggatgtttga gaaggtttta cagtaaaaga
     8761 a
//
LOCUS       DAVGJJ010000080         8717 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000080 DAVGJJ010000000
VERSION     DAVGJJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.080"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..2333
                     /gene="rnr"
                     /locus_tag="VVE35_001670"
     CDS             <1..2333
                     /gene="rnr"
                     /locus_tag="VVE35_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU3061823.1"
                     /translation="LQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMERKHQIRFEE
                     DGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAIDGDTVEVV
                     IKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQKISQPIYV
                     KKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVLESMDIVSE
                     FPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIKALKNGNLE
                     LGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSLNPQVDRLT
                     QSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHKIVPSIELM
                     AKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIESFMLMANE
                     TVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQEALQDIMRA
                     VEGEPYVDVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPDLLVHRMIR
                     DYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYVGEEYDAVV
                     SSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVGQQIRIRVE
                     RADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKSGRSNDKRK
                     HSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2296..2763
                     /gene="smpB"
                     /locus_tag="VVE35_001671"
     CDS             2296..2763
                     /gene="smpB"
                     /locus_tag="VVE35_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU3061824.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            2779..3639
                     /gene="tehB"
                     /locus_tag="VVE35_001672"
     CDS             2779..3639
                     /gene="tehB"
                     /locus_tag="VVE35_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU3061825.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            3712..4665
                     /gene="coiA"
                     /locus_tag="VVE35_001673"
     CDS             3712..4665
                     /gene="coiA"
                     /locus_tag="VVE35_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU3061826.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            4684..6495
                     /gene="pepF"
                     /locus_tag="VVE35_001674"
     CDS             4684..6495
                     /gene="pepF"
                     /locus_tag="VVE35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU3061827.1"
                     /translation="MVLQRNERNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVA
                     QYEGHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLY
                     SQLDQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELL
                     AGAGEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAY
                     QALYATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAA
                     VRKHLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGE
                     DYLSRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETG
                     HSMHSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLD
                     GFRGTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQY
                     EWARIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVM
                     RKAGVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6497..7210
                     /locus_tag="VVE35_001675"
     CDS             6497..7210
                     /locus_tag="VVE35_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU3061828.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7277..8218
                     /gene="prsA"
                     /locus_tag="VVE35_001676"
     CDS             7277..8218
                     /gene="prsA"
                     /locus_tag="VVE35_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU3061829.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8337..8678)
                     /locus_tag="VVE35_001677"
                     /pseudo
     CDS             complement(8337..8678)
                     /locus_tag="VVE35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2797 a         1632 c         1915 g         2373 t
ORIGIN      
        1 atttacaaga caagggaaag gtgactgtta atgatttggc tcaggctttg ggaaaagaca
       61 gttccaagga ttttcgtgag ttgattaaaa ccttgtcctt aatggaaaga aagcaccaga
      121 ttcgctttga agaagatggc agtctgacct tggaagccaa gaaaaaacat gagattaccc
      181 tcaaggggat ttttcatgcc cataaaaatg gttttggttt tgtcagccta gaaggggaag
      241 aggacgacct ttttgtgggg aaaaatgatg tcaactatgc tattgatggt gataccgtcg
      301 aggtagtgat taagaaagtc gctgaccgca ataagggaac agcagcagaa gccaaaatta
      361 ttgatatcct agaacacagt ttgacaacag ttgtcgggca aatcgttttg gatcaggaaa
      421 agcctaagta tgctggctat attcgttcaa aaaatcagaa aatcagtcaa ccgatttatg
      481 ttaagaaacc agccctaaag ttagaaggaa cagaagttct caaggtcttt atcgataaat
      541 acccaagcaa gaaacatgat ttctttgtcg cgagtgttct cgatgtagtg ggacactcaa
      601 cggatgtcgg aattgatgtt cttgaggtct tggaatcaat ggacattgta tccgagtttc
      661 cagaagctgt tgtcaaggaa gcagaaagtg tgcctgatgc tccgtctcaa aaggatatgg
      721 aaggtcgtct ggatctaaga gatgaaatta cctttaccat tgacggtgcg gatgccaagg
      781 acttggacga tgcagtgcat atcaaggctc tgaaaaatgg caatctggag cttggggttc
      841 acatcgcaga tgtttcctat tatgtgaccg agggttctgc ccttgacaag gaagccctta
      901 accgtgcgac ttctgtttac gtgacagacc gagtggtgcc aatgcttcca gaacgactat
      961 caaatggcat ctgctctctc aatccccaag ttgaccgcct gacccagtct gctattatgg
     1021 agattgataa acatggtcgt gtggtcaact ataccattac acaaacagtt atcaagacca
     1081 gttttcgtat gacctatagc gatgtcaatg atatcctagc tggcgatgaa gaaaagagaa
     1141 aagaatacca taaaattgta ccaagtatcg aactcatggc caagcttcat gaaactttag
     1201 aaaacatgcg tgtgaaacgt ggagctctca attttgatac caatgaagcg aagattttag
     1261 tggataaaca aggtaagcct gttgatatcg ttcttcggca gcgtggcatt gccgagcgga
     1321 tgattgagtc ttttatgttg atggctaatg aaacagttgc cgaacatttc agcaagttgg
     1381 atttgccttt tatctaccga attcacgagg agcctaaggc tgaaaaggtt cagaagttta
     1441 ttgattatgc ttcgagtttt ggcttgcgca tttatgggac tgccagtgag attagccagg
     1501 aggcacttca agacatcatg cgtgctgttg agggagaacc ttatgtagat gtattgtcca
     1561 tgatgcttct tcgctctatg cagcaggctc gttattcgga gcacaatcac ggccactatg
     1621 gactagctgc tgactattat actcacttta ccagtccaat tcgtcgttat ccagaccttc
     1681 ttgttcaccg tatgattcgg gattacggcc gttctaagga aatagcagag cattttgaac
     1741 aagtgattcc agagattgcg acccagtctt ccaaccgtga acgtcgtgcc atagaagctg
     1801 agcgtgaagt cgaagccatg aaaaaggctg agtatatgga agaatacgtg ggtgaagagt
     1861 atgatgcagt tgtatcaagt attgtcaaat tcggtctctt tgtcgaattg ccaaacacag
     1921 ttgaaggctt gattcacatc actaatctac ctgaatttta tcatttcaat gagcgtgatt
     1981 tgactcttcg tggagaaaaa tcaggtatca ctttccgagt gggtcagcag atccgtatcc
     2041 gtgttgaaag agcggataaa atgactggag agattgattt ttcattcgta cctagtgagt
     2101 ttgatgtgat tgaaaaaggc ttgaaacagt ctagtcgtag tggcagaggg cgtggttcaa
     2161 atcgtcgttc ggataagaag gaagacaaga gaaaatcagg acgctcaaat gataagcgta
     2221 agcattcaca aaaagacaag aagaaaaaag gaaagaaacc tttttacaag gaagtagcta
     2281 agaaaggagc caagcatggc aaagggcgag ggaaaggtcg tcgcacaaaa taaaaaggca
     2341 cgccacgact atacaatcgt agatacgcta gaggcaggga tggtcctgac tggaactgaa
     2401 atcaagagtg tacgagctgc tcgaattaat ctcaaggatg gctttgctca agtgaaaaat
     2461 ggagaagttt ggctgagcaa tgttcatatc gcgccttacg aagagggcaa tatctggaac
     2521 caggaaccag aacgtcgtcg taaactcctg ctccataaaa agcaaattca aaaattggaa
     2581 caagagacca aagggacagg aatgacctta gttcccctta aggtctatat aaaagatggc
     2641 tacgctaagc ttcttttagg acttgccaaa gggaagcatg actatgacaa acgggagtct
     2701 atcaaacgtc gtgagcaaaa tcgagatatc gcgcgtgtga tgaaagctgt taatcagcga
     2761 taaaaagagg aattgaaaat ggaaaaatta gttgcctata aacgcatgcc tttgtggaat
     2821 aaacaaacaa tgcctgaagc tgttcagcaa aagcacaata caaaagttgg gacttggggg
     2881 aaaattactg tcttgaaggg agctctcaag tttattgaat tgacagaaga aggggaagtt
     2941 ctagctgaac acctctttga agcaggggca gacaatccaa tggcccaacc tcaagcctgg
     3001 caccgagtgg aagctgccac agatgatgtg gaatggtact tggaatttta ttgtaaacct
     3061 gaggattatt ttgctaaaaa atacaatacc aatcctgttc attcagaggt cctagaggcc
     3121 atgcagacag tgaaacaagg gaaagctttg gatttaggtt gtggtcaggg gcgtaattct
     3181 ctttttctag cccagcaaga ttttgatgtg acggctgtag atcaaaatgg actagctctt
     3241 gaaatcttgc aaagcattgt ggagcaggaa gatttggaca tgcctgttgg cctttacgat
     3301 atcaattcag ctagcattga acaagaatat gattttatcg tttcaacagt tgttctcatg
     3361 tttctacaag cggaccgcat tccagctatt attcaaaata tgcaggagaa aaccagtgtt
     3421 ggtggttaca accttatcgt ttgtgccatg gacacggagg attatccttg ctcggttaac
     3481 ttcccattca cctttaaaga aggagaactg gcagactatt acaaggattg ggaattggtt
     3541 aagtacaatg aaaatccagg ccatttgcat cgtcgcgatg agaatggcaa tcgtattcaa
     3601 ctacgctttg cgaccttact agctaagaaa atcaagtaaa cacacatgaa gattaggaat
     3661 tttcctgatc ttttttcttt tttacgaatg atatagaaaa ggagggaatt catgtttgtt
     3721 gcgagagatg ctaggggaga attggtaaat gtgttagagg ataaacttga gaagcaagca
     3781 tacacctgcc cagcttgtgg aggccagctc catttgcgtc aaggaccaag tgtacggacg
     3841 cattttgccc ataaatcctt aaaagactgt gattttttct ttgaaaatga aagtccagaa
     3901 cacctggcca ataaggaatc cctctatcac tggttaaaaa aagagacaaa ggttcaatta
     3961 gagtacccgc tttcagaact taaacagatt gcggatgtat ttgtaaatgg caatctagct
     4021 ctagaagttc agtgtagtcc cttgcctcag aaagtcctta aagagcgaag tgagggatat
     4081 cgtagtcagg gttaccaagt actgtggttg ctgggtcaaa aactgtggct caaggagcgt
     4141 ttgactcgtc tacagcaagg ttttctttat ttcagtcaaa acatgggctt ttatgtttgg
     4201 gaattagaca aggaaaaaca agttttaaga ctcaaatacc tgatttacca ggatctccgc
     4261 ggtaaactcc attatcaaat caaggaattt tcctatggtc aaggtagttt attggaaata
     4321 ttgcgtcttc cctataagag acaaaaaata tctcatttta cagtttctga ggacaaggac
     4381 atctgtcgct atatccggca acaactttat tatcaaaatc tcttttggat gaaagaacaa
     4441 gcagaagcct atcaaaaggg agaaaatatc ctgacttatg gactgaaaga atggtatcca
     4501 caaattcgac caatagtggg caaatttttc cagattgaac aagacttgac tagctattat
     4561 cagcactttt atacctatta ccaaaaaaat cctcaaaatg attggcaaaa gctttatcca
     4621 ccagcctttt atcagcaata tttcttgaaa aatatggtag aatagaaagg atggaggaat
     4681 ctaatggtat tacaaagaaa tgaaagaaat gaaataaatg aaaaagatac atgggatcta
     4741 tcaacgatct acccaactga ccaggcttgg gaagaagcct taaaagattt aacagaacaa
     4801 ttggagacag tagcccagta tgaaggccat ctcttggata gtgcggataa cctactagaa
     4861 atcactgaat tttctcttga aatggaacgc cagatggaga agctttacgt ttatgctcat
     4921 atgaagaatg accaggatac acgtgaagct aagtatcaag agtactatgc caaggccatg
     4981 acactctaca gccagttaga ccaagccttt tcattctatg atcctgaatt tatggagatt
     5041 agcgaaaagc agtatgctga ctttttagaa gctcaaccaa agctgcaggt ttatcaacac
     5101 tattttgaca agctcttgaa aggcaaggat cacgttcttt cacaacgtga agaagaatta
     5161 ttggctggag ctggagaaat ctttggttca gcaagtgaaa ccttcgctat cttggacaat
     5221 gcggatattg tgttccctta tgtcctagac gatgatggta aagaagttca gctatctcat
     5281 gggacttaca cacgtttgat ggagtctaaa aaacgtgagg ttcgccgtgg tgcctatcaa
     5341 gctctttatg cgacttacga acaattccaa cacacctatg ccaaaacctt gcaaaccaat
     5401 gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca agagtgctcg tcatgcagcc
     5461 ctcgcagcga attttgttcc agaaagtgtt tatgacaatt tggtagcagc agttcgcaag
     5521 catttgccac tcttacatcg ctatcttgag cttcgttcaa aaatcttggg gatttcagat
     5581 ctcaagatgt acgatgtcta cacaccgctt tcatctgttg aatacagttt tacctaccaa
     5641 gaagccttga aaaaagcaga agatgctttg gcagtcttgg gtgaggatta cttgagccgt
     5701 gttaaacgtg ccttcagcga gcgttggatt gatgtttacg aaaatcaagg caagcgttca
     5761 ggtgcctact ctggtggttc ttacgatacc aatgccttta tgcttctcaa ctggcaagac
     5821 aatctggaca atctctttac tcttgttcat gaaacaggtc acagtatgca ttcaagctat
     5881 actcgtgaaa ctcagcctta tgtttacgga gattactcta tctttttggc tgagattgcc
     5941 tcaactacca atgaaaatat cttgacggag aaattattgg aagaagtgga agacgacgca
     6001 acacgctttg ctattctcaa taacttccta gatggtttcc gtggaacagt tttccgccaa
     6061 actcaatttg ctgagtttga acacgccatt caccaagcag atcaaaatgg ggaggtcttg
     6121 acaagcgatt tcctaaataa actctacgca gacttgaacc aagagtatta tggtttgagt
     6181 aaggaagaca atcctgaaat ccaatacgag tgggctcgca ttccacactt ctactataac
     6241 tactatgtat atcaatattc aactggcttt gcggccgcct cagccttggc tgaaaaaatt
     6301 gtccatggta gtcaagaaga ccgtgaccgc tatatcgact acctcaaggc aggtaagtcg
     6361 gactatccac ttaatgtcat gagaaaagct ggtgttgata tggagaagga agactacctc
     6421 aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt ttgaagccct tgttgaaaaa
     6481 ttaggattgg cataaaatgg ttgaatcgta tagtaagaat gctaaccata acatgcgtcg
     6541 tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag cgtcaaaagc aggtcacagg
     6601 tttcttgaaa gaattggaag actttgcccg caaggaaaat attcctatta ttccccatga
     6661 aacggttgct tatttccgtt ttcttatgga aaccatgcag cctaaaaata ttctggaaat
     6721 tgggacggct atcggttttt cagctctctt gatggctgaa catgcgccaa atgctaagat
     6781 tacaactatt gatcgtaatc cagaaatgat tggttttgcc aaggaaaatt ttgcccagtt
     6841 tgacagtcgc aagcaaatca ctctcctaga gggagatgcg gtggatgtct tatctacact
     6901 gacagagtct tatgatttcg tctttatgga ttctgccaag tctaaataca tcgtctttct
     6961 gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt gtcttggatg atatttttca
     7021 aggtggtgat gttgccaagg atattatgga agtccgtcgt ggtcagcgaa ccatttatcg
     7081 aggccttcaa aaattatttg atgcaacctt agacaatcca gaactcaccg caacattagt
     7141 gcctttagga gatggtattc tcatgcttcg taaaaatgta gcagatgttc aactgtctga
     7201 aagcgaatga ttttcagaaa aatttaagaa aaaatagtaa aatagataga gtaacactta
     7261 tctcaaagga gtagacatga agaaaaaatt attggcaggt gccatcacac tattatcagt
     7321 agcaacttta gcagcttgtt cgaaagggtc agaaggagca gaccttatca gcatgaaagg
     7381 ggatgtcatc acagaacatc aattttatga gcaagtgaaa aacaaccctt cagcccaaca
     7441 agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa caatatggct cagagcttga
     7501 tgataaagag gttgatgata ctattgccga agaaaaaaaa caatatggcg aaaactacca
     7561 acgtgtcttg tcacaagcag gtatgactct tgaaacacgt aaagctcaaa ttcgtacaag
     7621 taaattagtt gagttggcag ttaagaaggt agcagaagct gaattgacag atgaagccta
     7681 taagaaagcc tttgatgagt acactccaga tgtaacggct caaatcatcc gtcttaataa
     7741 tgaagataag gccaaagaag ttctcgaaaa agccaaggca gaaggtgctg attttgctca
     7801 attagccaaa gataattcaa ctgatgaaaa aacaaaagaa aatggtggag aaattacctt
     7861 tgattctgct tcaacagaag tacctgagca agtcaaaaaa gccgctttcg ctttagatgt
     7921 ggatggtgtt tctgatgtga ttacagcaac tggcacacaa gcctacagta gccaatatta
     7981 cattgtaaaa ctcactaaga aaacagaaaa atcatctaat attgatgact acaaagaaaa
     8041 attaaaaact gttatcttga ctcaaaaaca aaatgattca acatttgttc aaagcattat
     8101 cggaaaagaa ttgcaagcag ccaatatcaa ggttaaggac caagccttcc aaaatatctt
     8161 tacccaatat atcggtggtg gagattcaag ctcaagcagt agtacatcaa acgaatagtc
     8221 caaatcaatg agtcagggaa aaaactcgac ttcaggaaaa aatgaagcaa acattcccac
     8281 aataaaacgc atagtacaag gtttgtactg cccccaaaaa aagttagaca attaatttat
     8341 ccgaaggatt tagttctgta ttgcacagag ctaagtcctt ttagttttat cttaattctc
     8401 ttattgttgt aataatcaat atagtctata atggcttgtt ccaattgatt aagtgattta
     8461 aatgtttgct catagccata aaacatttcg gattttaaaa tgccaaagaa agattccatc
     8521 ctaccgttgt cttggctgtt tcctttgcgt gacatagatg cttgaattcc cttactctct
     8581 aggaagcgat gataagaatc gtgttggtat tgccagcctt ggtcactatg gagaatcgta
     8641 ttctcgtagt gcttctcttt gaatgcctgt tccaacatta acgatcaatc aatttaatca
     8701 tgtacctaag attagaa
//
LOCUS       DAVGJJ010000081         8540 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000081 DAVGJJ010000000
VERSION     DAVGJJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.081"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            169..468
                     /locus_tag="VVE35_001678"
     CDS             169..468
                     /locus_tag="VVE35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU3061830.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            539..2560
                     /locus_tag="VVE35_001679"
     CDS             539..2560
                     /locus_tag="VVE35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU3061831.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLPSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYEDGIKLLKNAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2562..3203
                     /locus_tag="VVE35_001680"
     CDS             2562..3203
                     /locus_tag="VVE35_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061832.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3307..4659
                     /locus_tag="VVE35_001681"
     CDS             3307..4659
                     /locus_tag="VVE35_001681"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU3061833.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEALIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGAFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4661..5719
                     /locus_tag="VVE35_001682"
     CDS             4661..5719
                     /locus_tag="VVE35_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU3061834.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNILDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVSPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLTHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5729..6895
                     /gene="divIB"
                     /locus_tag="VVE35_001683"
     CDS             5729..6895
                     /gene="divIB"
                     /locus_tag="VVE35_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU3061835.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKATIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEKSN"
     gene            7161..7862
                     /gene="pyrF"
                     /locus_tag="VVE35_001684"
     CDS             7161..7862
                     /gene="pyrF"
                     /locus_tag="VVE35_001684"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU3061836.1"
                     /translation="MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            7896..8528
                     /locus_tag="VVE35_001685"
     CDS             7896..8528
                     /locus_tag="VVE35_001685"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU3061837.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQKNWQEG"
BASE COUNT         2633 a         1553 c         1908 g         2446 t
ORIGIN      
        1 atagaggcaa tgtagtaaaa tatctaaaaa attcgatttt ttccttgaca acaaataata
       61 aaaatgttat tcttatcaca taagaaagcg gtttcaataa aagagagagg agggtttatt
      121 ttgttgcatt gttgctcaag gtgtgctttt tttgaaagaa aaaataaaat gaaaacgaaa
      181 aaacatagat tacttgctct agctcttatt tcaagtttta cattattggg agctgcatca
      241 gctgctgtac aatatccaga tggaggagta tggacgtatg gagaaggttc aggaggtggt
      301 tgggcttttt caaattacta tcatggtaaa aaatatcatt attcttctct tgtaagtaga
      361 tggaatagtc attcagataa aggagaagct cctgctggaa aaacctccta tgcatggatt
      421 tggactaaat ggggagaaca agtagcattt tactgtgatt atgactaatc gttcattgat
      481 ttaaatcgtt ccccttgtaa gaaattacag ggggaatttt gtttacaagg agtctactat
      541 gaagaaacta ttcatattat tatcaacttt ttttctcagc ttcttccttg cttggattat
      601 tgtcttacgt gcgccacaat atttatatgc aagctatgat tccgtttctt tacttcgtgt
      661 caaaaaagat actcaggaac cgacgcgtga ggtatttgaa caggaattgg agaattttgc
      721 aaactcagaa cagagtttaa tagctagaag aattgtagag ccgagtaagg atgggacgac
      781 tcactttact tatgcaactt atggtcaggg aactttacca aaagaattcc aagaagctag
      841 ccaagaaagt cgtgaacgta gtgatccgct aaatagttat ctccttttgt caggctcctt
      901 gacgaaagaa aagcttgccg ataaattagg agatttgggt tataaagcaa gtgctgaccg
      961 aaagataccg ccctattttc ttgcttttcg aatattacta aatcccctta ttttaattag
     1021 tttagcaata tttggcttat ctttctttgc tttagtgatt atcactcgga ttaaggaaat
     1081 gagagcagca ggtataaaac tcttttctgg tcagactctc ttatccatca tggggcattc
     1141 tttatctact gatatcaaat ggctccttcc atcagccctc ctttccttcc taggtggggg
     1201 agtcgttctt tttagtcaag gtttgtttta tcctatcttg ttagccacct atggttttgg
     1261 gattagtttc tatctgttgt ttttattggc gatttcaatt ttactaatgc tcctttatct
     1321 aatgagtttg aattacaaag cattagttcc cgttattaag gggagattac cccttaaacg
     1381 cctgatgatt ttaaccctat tgtgtcagtt ggttgctgtc tttacagtag gatacgctgt
     1441 taagacaggt ttgacgtctt accaacgatt gaaagaactt gaaatttcaa aacaagcatg
     1501 gcaggataga gcagactatt atcagatttc ctttggctta ggtgatagag gaaaagatac
     1561 agaaaatcag agcaagtggt atgcctttgc caaggaagca atcgaagaag aacaagctct
     1621 ttatgtaaag gataatctgc tccattttgc caatccacaa ggaaaaaatg aacagggaga
     1681 gacactggat acctatagtc cagatgctaa tacgctctat gttagtccca gttatttgga
     1741 caaggaaaag gtcgtggtag atgctgagac caaacagaag ttagcccatc tccaaaaagg
     1801 tgagtttatc ctcttgctcc cagaacattt gcgctctcga gaagcagaac ttaagaaagt
     1861 ttttgaagaa agattgagtt attatggaaa atctggtgag gaggcaagtg ctcctttgga
     1921 ttatgagatg aaagcgcacg ttagttatct ttcaatggga gaaaagcggt ttgtttataa
     1981 taacggtgag aatcccgtat ctactcagta tttgactgat ccgattttag tggtattcac
     2041 gccgacttct acaggtgata gttttatatc cttatctagt tggtctatca atgctggaaa
     2101 acaactcttt atcaaaggat atgaagatgg gattaaactc ttgaaaaatg caggaattta
     2161 tgagcaagta tcctatctta aagaaggaag aagtgtttat ctaactcgtt ataatgaagt
     2221 tcaaactgaa acagcaactt taatcctagg agctattgtt ggaatagcca gttccttgtt
     2281 actcttttat tctgtcaatc ttctatattt cgagcaattc cgccgagata tcttgattaa
     2341 acgaatttca ggtttacgat tttttgaaac acatgctcag tatatggtta gccaatttgc
     2401 cagttttgta tttggtgcta gtctctttat tttaagcagt cgagacttgg tgattggctt
     2461 gctcacttta ttggtatttc tagctagtgc agttttgacg ctttaccgtc aagcgcagaa
     2521 agaatctcgt gtttctatga caattatgaa aggaaaatag gatgattgaa ctaaagaata
     2581 tatctaaaaa atttggaagc cgtcagctat tttcagatac gaatcttcat tttgaaggtg
     2641 ggaaaattta tgccttaatc ggtacaagtg gctgtggtaa gacaacactc ttgaatatga
     2701 ttggacgatt agagccatat gacaaagggc aaatcatcta tgatggcact tctcttaagg
     2761 acatcaagcc ttctgttttc tttagagatt acttgggata cttatttcaa gattttggct
     2821 taattgaaag ccaaaccgtc aaagagaatc tcaatctggg tttagttggt aaaaagttga
     2881 aggaaaaaga gaaaatctct ttgatgaaac aagctctaaa ccgtgtaaac ctctcttatt
     2941 tggatttgaa gcaacctata tttgagttat caggaggaga agcacaacgt gttgcactag
     3001 cgaagataat tttaaaggat ccacctttga tcctcgcaga tgaaccaacc gcttcactag
     3061 accccaaaaa ctctgaggaa ttactttcca tcctagaatc tttaaaaaat ccgaatcgga
     3121 ccattattat tgcgacccac aatcctctga tttgggagca agtggatcag gtcattcgag
     3181 ttaccgattt atcacataga tgatatggta agattcagtt agaagaaaga gtcacaaaca
     3241 cactttgtgg cttttttatt tccataaaaa tggtaaaata gtaggagtag aaatggagtt
     3301 cgagacatga aagtaataga tcaatttaaa aataagaaag ttcttgtttt aggtttggcc
     3361 aagtctggtg aatctgcggc tcgtttgttg gacaagttag gtgccattgt gacagtaaat
     3421 gatgggaaac cattcgagga caatccagct gcgcaatgtt tgctggaaga agggatcaag
     3481 gtcattacag gtggccatcc tttggaactc ttggatgaag agttcgccct catggtaaaa
     3541 aatccaggta tcccctacag caatcccatg attgaaaagg ctttggccaa gggaattcca
     3601 gtcttgactg aggtggaatt ggcttatttg atttcagaag cactgattat tggtatcaca
     3661 ggatcgaacg gtaagacaac cacaacgact atgattgggg aagttttgac tgctgctggg
     3721 caacatggtc ttttatcagg gaatatcggc tatcctgcca gtcaggttgc tcaaatagca
     3781 tcagataagg acacgcttgt tatggaactt tcttctttcc aactcatggg tgttcaagaa
     3841 ttccatccag agattgcggt tattaccaac ctcatgccaa ctcatatcga ctaccatggg
     3901 gcattttcgg aatatgtagc agccaagtgg aatatccaga acaagatgac agcagctgat
     3961 ttccttgtct tgaactttaa tcaagacttg gcaaaagact tggcttccaa gacagaagcc
     4021 actgttgtac cattttcaac acttgaaaag gttgatggag cttatctgga agatggtcaa
     4081 ctctacttcc gtggtgaagt agtcatggca gcgaatgaaa tcggtgttcc aggtagccac
     4141 aatgtggaaa atgcccttgc gactattgct gtagcaaagc ttcgtggtgt ggacaatcaa
     4201 accatcaagg aaactctttc agccttcggt ggtgtcaaac accgtctcca gtttgtggat
     4261 gacatcaagg gtgttaaatt ctataacgac agtaaatcaa ctaatatctt ggctactcaa
     4321 aaagccttgt caggatttga caacagcaag gtcgtcttga ttgcaggtgg tttggaccgt
     4381 ggcaatgagt ttgacgaatt ggtgccagac attactggac tcaagaagat ggtcatcctg
     4441 ggtcaatctg cagaacgtgt caaacgggca gcagacaagg ctggtgtcgc ttatgtggag
     4501 gcgacagata ttgcagatgc gacccgcaag gcctatgagc ttgctactca aggagatgtg
     4561 gttcttctta gtcctgcaaa tgccagctgg gatatgtatg ctaactttga agtacgtggc
     4621 gacctcttta tcgacacagt agcggagtta aaagaataat atgaaaaaaa ttgtctttac
     4681 aggtgggggg acggttggac acgttaccct caatcttttg ttaatgccca agttcatcga
     4741 agatggttgg gaagtccact atatcgggga caagcgtggt atcgaacacc aagaaatcct
     4801 taagtcaggt ttggatgtca ccttccattc tattgcgact ggaaaattgc gtcgctattt
     4861 ctcttggcaa aatatactgg atgtcttcaa agttggttgg ggaattgtcc aatcgctctt
     4921 tatcatgttg cgactgcgtc cacagaccct tttttcaaag gggggctttg tctcagtatc
     4981 gcctgttatc gcagcgcgtg tgtcaggagt gcctgtcttt attcacgaat ctgacctgtc
     5041 tatgggcttg gccaataaaa tcgcctataa atttgcgact aagatgtatt caacctttga
     5101 gcaagcttcg agtttgtcta aggttgagca tgtgggagca gtgaccaagg tttcagatca
     5161 aaaaaatcca gaaccagatg aattggtgga tattcaaacc cactttaatc ataaattgcc
     5221 gactgtattg tttgttggcg gttctgcagg tgctcgtgtc tttaaccaat tggtgacaga
     5281 ccataagaaa gaactaacag agcgctacaa tattatcaat ctaactggag attctagcct
     5341 gaatgagttg agccaaaatc tttttcgtgt tgactatgtg accgatctct atcaaccctt
     5401 gatggaattg gctgatattg ttgtgacacg aggtggtgcc aatacgattt ttgagctctt
     5461 ggcgatagca aaattgcatg tcattgtgcc gcttggtcgt gaagctagtc gtggtgacca
     5521 gattgaaaat gcagcttact ttgtaaaaaa aggctatgca gaagaccttc aagaaagcga
     5581 tttgaccttg gatagtttgg aagagaagct tactcactta ctaagtcaca aggaagatta
     5641 ccaagctaag atgaaagctt ctaaggaatt gaaatctcta gcagattttt atcaattgtt
     5701 gaaaaaagat ttatcataag gaaagtaaat gtcaaaagat aagaaaaatg aggacaaaga
     5761 aatcctcgaa gaattgaaag agttatcaga atggcagaaa cgaaaccaag aatatctaaa
     5821 aaagaaggct gaagaagagg cggctctagc tgaggagaag gaaaaggaaa gacaagctcg
     5881 aatgggagaa gaatctgaga agtcagagga taaacaggac caggagagtg aaacagacca
     5941 ggaagattca gaatcagcta aggaagagtc tgaagaaaaa gtagcatcct cagaggctga
     6001 caaagagaaa gaagagaaag aagaaccaga gtctaaagag aaggaggaac aggataaaaa
     6061 gctttctaaa aaggctacaa aggaaaaacc agccaaagca aagattcctg gtatccatat
     6121 cttgcgagcc ttcacgattt tatttccaag tctgctttta ttgattgtct ctgcctactt
     6181 gctcagtcct tatgcgacca tgaaagatat tcgtgttgag ggaacggtgc aaactacagc
     6241 tgatgatatt cgacaggctt caggcattca ggattcggat tatacgatta accttctgct
     6301 agacaaggca aaatatgaaa agcagattaa gtctaactat tgggttgaat cagctcaact
     6361 tgtctatcaa tttccaacta agttcactat taaggtcaag gaatatgata ttgtggccta
     6421 ctatatttct ggtgaaaatc attatcctat tctttccagt ggtcagcttg agactagttc
     6481 tgtgagtctg aacagtttac cagaaactta tttatcagtt ctctttaatg atagtgaaca
     6541 aatcaaggtt tttgtctcag aacttgctca aattagccca gaactcaagg cgactatcca
     6601 aaaggtggaa ttagccccaa gcaaggtgac atccgattta attcgattga ccatgaatga
     6661 ttcggacgaa gtcttggttc ctctatctga aatgagtaag aaactgccat attacagtaa
     6721 gattaagcct caattgtcag aaccgagtgt ggtcgacatg gaagctggaa tctacagcta
     6781 cactgtggcg gataaattaa ttatggaagc tgaggaaaaa gccaaacaag aggccaagga
     6841 agccgagaaa aaacaggaag aagaacagaa aaaacaagag gaaaagagca attgaaatca
     6901 aacgacccag cgctcattgc gtcgctaggt ttaccttttc tcttatagtt ctttagtgac
     6961 catgttttta cttgacaagt gctagtagaa ataatagaat aatagaaaac ctttaaagca
     7021 gtccagagag gcagctaagg ttagacggtg aaagggtgga gactacccat ttttcgtgga
     7081 accttgctgt tggcaggttc cttttttcgt ggcttctgtt ggccagactc tctcactagt
     7141 aaaggtaaaa ggagaaacct atgcgagaac atcgtccaat cattgctctt gattttccta
     7201 gttttgaggt ggtcaaggaa tttttagctc ttttcccagc agaagaaagc ctttatctca
     7261 aggtagggat ggagctttat tacgcagcgg ggcctgagat tgtgtcctac ttaaaaggtt
     7321 tgggtcatag tgtctttttg gatctcaaac ttcatgacat tcccaataca gtcaagtcag
     7381 caatgaagat cctatctcag cttggtgtgg atatgaccaa tgttcatgcg gctggtggtg
     7441 tagagatgat gaaggctgcg cgtgaaggtc ttggaagtca agctaaattg atcgctgtaa
     7501 ctcagctcac atcaacgtca gaagctcaga tgcaggagtt tcaaaatatc caaaccagtc
     7561 tgcaagagtc tgtgattcac tatgccaaga agacagctga agctggcttg gatggtgttg
     7621 tttgctcggc tcaggaagta caagtcatca agcaggctac caatccagat tttatctgtc
     7681 tgacaccagg gattcgtcca gctggtgttg cagttggaga tcaaaaacga gtcatgacac
     7741 ctgctgatgc ctatcaaatc ggcagtgact atatcgtagt gggacgtccc attacccaag
     7801 ctgaggatcc tgttgcagct tatcatgcca tcaaggatga atggacacag gactggaatt
     7861 aaagaactag attagaaaaa taaaaggaga ataccatgac acttgctaaa gatatcgcta
     7921 gccacctctt gaaaatccaa gccgtttacc tcaaaccaga ggaacccttc acttgggcat
     7981 ctggtatcaa gtcaccgatt tacactgata atcgtgtgac actagcctat ccagaaactc
     8041 gtaccctaat tgaaaatggt tttgtggatg ctatcaaaga agcctttcct gaagtagaag
     8101 tgattgcagg aactgcaaca gcagggattc cacacggagc cattattgct gataagatgg
     8161 acttgccttt tgcctacatc cgtagcaaac caaaagacca tggagctggt aatcaaatcg
     8221 aaggtcgcgt agctcaaggt caaaaaatgg tagtggttga agaccttatt tcaacgggtg
     8281 gttcagttct tgaagctgta gcagcagcca agcgagaagg agcagatgtg cttggagttg
     8341 tagcgatttt cagctaccaa ttgccaaaag cagataagaa ctttgcagat gctggtgtta
     8401 aacttgtgac gctttcaaac tatagcgagc ttatccatct agcccaagaa gaaggttaca
     8461 tcacgccaga gggccttgat cttctaaaac gctttaaaga agaccaaaaa aattggcaag
     8521 aaggttaggt cagtaagata
//
LOCUS       DAVGJJ010000082         8328 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000082 DAVGJJ010000000
VERSION     DAVGJJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.082"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(108..311)
                     /locus_tag="VVE35_001686"
     CDS             complement(108..311)
                     /locus_tag="VVE35_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061838.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(304..1386)
                     /locus_tag="VVE35_001687"
     CDS             complement(304..1386)
                     /locus_tag="VVE35_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU3061839.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1512..2294)
                     /locus_tag="VVE35_001688"
     CDS             complement(1512..2294)
                     /locus_tag="VVE35_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU3061840.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGTHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2294..3637)
                     /locus_tag="VVE35_001689"
     CDS             complement(2294..3637)
                     /locus_tag="VVE35_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU3061841.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3736..5052)
                     /locus_tag="VVE35_001690"
     CDS             complement(3736..5052)
                     /locus_tag="VVE35_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU3061842.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5246..6472
                     /locus_tag="VVE35_001691"
     CDS             5246..6472
                     /locus_tag="VVE35_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU3061843.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLITGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6671..8245
                     /locus_tag="VVE35_001692"
     CDS             6671..8245
                     /locus_tag="VVE35_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU3061844.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2379 a         1617 c         1856 g         2476 t
ORIGIN      
        1 ggggtcagta cagaaaaact ttgatactat gcgttttatt gtgggaagat tttcctcatt
       61 ttctagtgaa attgaacttt ttctaacatc ttttgataga tgacttctta atcaattatc
      121 atttccattg ttgtttcttg gggtttcatc ccttgtcttt gataaaagcg gagagcaccc
      181 tcgttatcat tccagacact tagggttaga ttattgcagc caatttcttt agcataatag
      241 cagaaaaatg acaataaaat agcccctaga tattttgaaa ggaaagcata ggggctatat
      301 agtttaatca aaataaagca aatctttgct ggtgcttgta aagaggtcga agccatcctt
      361 ggtaacaaca ccgcagtctt caatacgaac accgacttta ccagggatat agatacctgg
      421 ttcaacagag aagcacatgc cttcttcgat gaccatgtcg tttccttcca tgatagatgg
      481 gaattcatgg acatccatac cgataccatg cccgagacgg tggttgaagt actcaccata
      541 accagctttt tcgatgacct cacgggcagc gcggtccact tcatgagcag tcacacctgg
      601 cttgataaag tcaagagcag cttgttgggc ttcaagagtc aagttgtaaa tatctttctt
      661 gaattggtct ggtttgccga cagcgactgt acgagtcata tctgacgcat agccattgac
      721 cagaacaccc aggtcaaaga ggagaagagc atcattttca accttattag ctgctggaat
      781 gccgtgtgga ttcgcagcat tatcaccagt caagaccatg gtatcaaagc tcatttcgta
      841 accttcacgt ttcatggcaa agtcaatttg tgcgatgata tctgtctcag tcttatcaag
      901 agaaatattg tcaaaaccaa catgaacagc cttgtcagca taaagacctg caaccatcat
      961 tttttgtact tcatcagctg atttgatgag gcgcatgcgt tggatacgag gagtgaggtt
     1021 ttcaaactca gcagtttcaa aaactgtttt caaaccatgg tatttggtca agatgagatt
     1081 gtcaaactca acagcgacac gtttgaagtc aagttgtgga agagcatgtt tgattttttg
     1141 ccatggattt tcagaatcga catagcccac tactgggaag gaaacggtgc tacttgcacg
     1201 ttctacttca agagctggga caaagaggag aggttcctga tccgctagga caaagaggaa
     1261 catttggcgt tcatggggat cactgtaaaa accagtgagg taattgattg tgacggggtc
     1321 agatacgaca gcgacgtcta gtttttctga ttcaagatat gttaggattt gttgtaattt
     1381 agacatatgc taccttcttt ctacccctct attttggcaa aaaatgagag aaaatgcaag
     1441 ggagaagggt aaaagaaaag acaaaaaact ccgacaagat aacggagttc tttgatatca
     1501 ttttcctttg tttaagaaaa gtcagccttg cttttgacgt cactgtactc ttcagcgatt
     1561 tcttggctga gaatgtcctc ataggcaggg agttggatgt cctgaccata tttcttcttg
     1621 agggcagtag taactaggcg ataagccaga ttggcattac gagagaggat aggcccgtgg
     1681 aagtaggaac caaagacatt cttataatga accccttcac cgaccttttc ttcgttgttt
     1741 ccatttccat agacaacctg tcccagcggt ttttggtcat cagagaggaa agtacgacct
     1801 tggtgatttt caaatccata gtaggtttca tcgaaatctt cattgtgaat cttgatgtca
     1861 ccgataaaac ggttattggt ctggttgagc gtgtagtgtc ccatgacccc tagcccttcg
     1921 atacgttttc ctgaagcttc aacataatat tgacccaata gttggaaacc accgcagata
     1981 gccagaacta caccgtcgtt ttggatgtag ttgtcaatgc tctctttttt agcaggtagg
     2041 tcgtctgcaa tgatactttg ttcaaagtct tgaccaccac cgaaaaaggc gatgtcgtag
     2101 tgattttcat caaagtcatc atggagagaa acgatgtcaa cggtcacatg ggttcccagt
     2161 ttttcagcca catacttgag catgaggatg tttccattgt ccccgtaggt attcatgaga
     2221 tttccgtaga ggtgggcaat gttgagctga taggggtaat tgccatcttt tgaggaaagt
     2281 gaagtataaa ccattagttc atctcctttc taacaatctg acgactagcc agcagttcac
     2341 gaaattccag catggcagta taagttgcca gaatataggc atgcttgcag tcttgattct
     2401 caatggtctt gagaacttgc tccagattac tcgtttcagt gattttctca gctggatagc
     2461 cagtcactcg gaggcgacga gcgatttcag aatgacgaac accgccagcg ttgatttcag
     2521 gaatgtccat gtcagtgatt tgctcaaagt ctgcatccca gatccagcta gtgtcaattc
     2581 catctgcata gttggcatta aggaggacag ataggctaaa tggataaggt gctagtttga
     2641 tcatttcgat agcttgggtt gcaccgactg gatttttaat caagacaagg gtacattcct
     2701 tgtcaccgat atgaaaggtt tcttggcgtc caaagacagc acggctcttg tcaaatccct
     2761 gtttgatgag ttgggaatct gcgccgagga aacgggcgat ggccacagca gctagggcgt
     2821 tatagatatt atagagcccg ccgatttgga taccgtattc ttggccgtct atgacaaagc
     2881 gagagcgatt gttggtcaac tcaaccagtt ttgtcaaacg atagtcgaga tcaggacgtt
     2941 tacatccaca accctcacag atataggcac ccaagtttgc ataggtatta tgctcatatt
     3001 tgaggatgcc ttggcagtca ggacagagaa tcccttcggt attgtagtga gccagttggg
     3061 ctggtccttt ttccaagtca aaaccaaaat actctatagg gtttggaata gttggcttgt
     3121 agaaaagtgg actgtctccg ttaaggagaa cagtagcagt tggaactttc cgaatggcat
     3181 ccaatatcat gttataggta gtatagattt caccgaaacg gtccatctgg tcacggaaga
     3241 tattagtaat gacaaaaaga ctaggctgga tatagtcaca gatacgagat agactggctt
     3301 cgtcaatttc gaggacggca atattttttc cagttttaga agatttggct gttaggaagg
     3361 ttgttgcaat ccctgtaatc atgttggcac cgcttggatt ggttagaact tgaccataga
     3421 cctcttttaa aatgccgaca gtgagggcag ttgtcagggt ttttccattt gttccagtga
     3481 caacgacaat ctcgtagttc ttagctaggt tttgtaaaat atctttatca aattgaaggg
     3541 cgactttccc tgggagcgta cttccacgtc caagacggct taaaacgaag tgggaagaac
     3601 gcccagcaag aaggcccaaa gtagttttta agttcatgtt tctattatat cacaaaagag
     3661 ggaaaagaca gatttggtgt ttcgtagaag taaaaacagc ccaaagaggg ctgtcagtta
     3721 agatgagatt tcaacttaaa tcgcaaacaa gtcattcaag ttctcagcca aggttgggtg
     3781 agtgaagatt tgttttgtga agtaagtgta aggaatcttg ttgtccatag caacagtaat
     3841 gatgttgatg atttcttgag aaccttctga gaagatgctt gcaccaagaa tttcttttgt
     3901 ttcagtatta acaacagctt tgaaagctcc gcgaaggtct ccatttacgt gaccacgagg
     3961 catggctgca acagggattt ctttcactgc gtatggaagt ttcaaatcag ctgcttggct
     4021 ttcagtcaaa ccaacttgtg aaagtgcagg tgtgatgaac atagtatttg gcacattgag
     4081 acggtcctca agtgtgtagc tgccatctcc agcaaggtag ctgtaaacaa cacggaagtc
     4141 atcaagtgaa atgtaagtaa attgaagacc accgttgaca tctccaactg caaagacacc
     4201 aggaacgttt gtttgacagt gtttatctac tttaatagcg ccacgttcag ttagttcaat
     4261 atctgtattt tcaagttgaa gtggttctac atttggtttg cgtccagttg cgtagagaag
     4321 ggcgtcgaaa cggtaagttt cgtcttcagt tacgacaagc acttggtcac cgtcgttttt
     4381 aatttcagta gtatggatat tttgaagcaa ttcaataccg tcttcttcca tgtattgttt
     4441 agcaagagct gcgatggaag gttctgcacg aggtaggaat gtatccaagg catctaggac
     4501 tgtaaccttg cttcctagtt tattgtaaag gccagcaaat tcaagaccga tatttccgcc
     4561 accaaggact ccaagttttt caggcaattt atccaagctt tggatacctg ttgagtcaaa
     4621 gacgtttttg cttgtagcaa gtccagggat tggcaagacg tttgaaacag caccagtgtt
     4681 gatgacgata gtttcagcag tcaattcttg tttttcatca ccagcttgga tttcgatgac
     4741 tttatttgaa aggaagtgag cttcagcatc aaagatattg acgccagtac cagcaacagt
     4801 cgtatagttt ttaccgttga ggcgaccagt gatcgtgttt ttggtagcaa tgacttcttc
     4861 aaaagacaag tctttctcag cagcaaccag caaagtttta gttgggatac aaccgatgtt
     4921 gatacaagtt ccaccgtaca tagctttgct acgttcaacg agggcaactt ttttgccagc
     4981 tgaagccaat ttacctgcta gtgttttacc agctttacca aatccgataa cgattaaatc
     5041 ataagttaac atttagagtt cctcctattc gcatttcatt ctagcacaag aaaaaaactt
     5101 ttgcacaatt ctgctacgga aaagacagaa aaagataaaa acgctttcca gacaaaaaac
     5161 tagtaatttt ttgacaagct tgataaacta aaatcctatc tatttttata taagatagaa
     5221 aaaatgttcg aaaataggag gttttatgaa atcgaatcgt tatattattg cctttgctgg
     5281 ggtcatttta cacttaatgc tgggttcgac ttatgcttgg agtgtttatc gtaaccctat
     5341 tattgaaaaa acgggatggg atcaggcttc tgttgccttt gcctttagtc tagcaatctt
     5401 ttgtttgggc ttatcggctg catttatggg gcgtttggta gaaaaatttg gtccgagagt
     5461 catggggagt ctatctgctt ttctatacgc aggtggaaat atcttaacag gatttgcaat
     5521 agaccgtcag gaactgtggt tgttgtatct agcttatggc attttaggtg ggcttggttt
     5581 gggagcaggc tatattaccc ctgtgtcaac gattataaaa tggtttcctg ataaacgtgg
     5641 tctcgcaaca ggtttagcga ttatggggtt tggttttgct tctttattga ctagtcccat
     5701 agcgcaacat ctcatcacag gggtagggct tgtagaaact ttttatattt taggtgcaag
     5761 ttactttatt atcatgctcc tagcttcaca attcattaag cgtccaaatg agcaagagct
     5821 tgcaatttta tctgcttcag ggaaagaaaa aacagactct ctgacgcaag ggatgactgc
     5881 aaatcaggct ctaaaaagca atcggtttta tatactttgg attattttct ttatcaacat
     5941 agcttgtggt ttaggcttaa tttcagcggc atcgccaatg gcacaggaga tggctggctt
     6001 gtctacaagt catgcagcag taatggtggg cgttttaggg attttcaatg gatttggtcg
     6061 cttgctctgg gcgagcttat ctgactatat cggtcgccct ctaaccttta gtatattact
     6121 gcttgttaat cttttctttt ctctctcact ttggctcttt acagattccg ttttatttgt
     6181 agtcgctatg tctattttga tgacttgcta tggagctggt ttttcattga tcccagctta
     6241 tctcagtgat atttttggaa ccaaggaatt ggccgctctg catggatata ttttaacagc
     6301 ttgggcaatg gctggtttag cgggacctat tttattagca gagacttata aaatggctca
     6361 ttcgtacaca caaaccttgt tcgtttttct cattttatac agtatcgcct tggctttgtc
     6421 ttattatcta ggtcgttcaa tcaaaaaaga aagtcaaaaa ccgcttacat gattttctgt
     6481 agaaatgggg ctatcttttg ctctggtctt gtgttatact agataggttg caaagaaaac
     6541 agtacttttc ttttgtggaa aagaagcaac acgattttat atctagttat atgaaaatac
     6601 gtcataaaaa gaaaagtata aactaagccc tatagggtgg cttcgcacca cctttagaaa
     6661 gaagaataac gtgaaattta atgaattaaa cttgtctgct gatttgctgg cagaaattga
     6721 aaaagctggt tttgtagaag ctagtcctat ccaagaacaa actattcctt tggcccttga
     6781 aggcaaggat gttatcggtc aagctcagac tggtacagga aaaactgcag cctttggctt
     6841 gcctaccctt gaaaaaatcc gtacagaaga agcgactatc caagccttgg tcatcgctcc
     6901 aactcgtgaa ctagctgtcc aaagtcaaga agaactcttc cgctttggtc gtagtaaggg
     6961 agtcaaagtc cgttcagtat atggcggatc aagcattgaa aaacaaatta aggctcttaa
     7021 atctggtgcc catatcgtgg tgggaactcc aggtcgcctc ttggacttga ttaaacgcaa
     7081 ggccttgaaa ttacaaaaca ttgaaaccct tatccttgac gaagcggatg aaatgcttaa
     7141 catgggcttc cttgaagaca tcgaagccat tatttcccgt gtacctgaga accgtcaaac
     7201 tttgcttttc tcagcaacta tgccagatgc catcaaacgt atcggtgttc agtttatgaa
     7261 agcccctgaa catgtcaaga ttgcggctaa ggaattgaca acagaattgg ttgaccagta
     7321 ctatatccgt gttaaggaac aagaaaaatt tgacaccatg actcgtctca tggatgtggc
     7381 acaaccagaa ctcgctattg tatttggtcg taccaaacgc cgtgtggatg aattgactcg
     7441 tggtttgaaa attcgtggct tccgtgcaga aggaattcat ggcgacctag accaaaacaa
     7501 acgtcttcgt gtccttcgtg actttaaaaa tggcaatctt gatgttttgg ttgcgacaga
     7561 cgttgcagcg cgtggtttgg atatttcagg tgtgacccat gtctacaact acgatattcc
     7621 acaagatcct gagagttatg ttcaccgtat cggtcgtaca ggtcgtgctg gtaagtcagg
     7681 tcaatctatt acttttgttg ctccaaacga aatgggttac cttcaaatca ttgaaaactt
     7741 gactaagaaa cgcatgaaag gtctcaaacc tgcaagtgta gaagaatcct tccaatcaaa
     7801 aaaacaggta gctctcaaga aaatcgaacg tgattttgca gatgaaacca ttcgtgccaa
     7861 ctttgagaaa tttggtaagg atgctcgcaa attggctgct gagtttactc cagaagaatt
     7921 ggcaatgtat atcttgagtc tgacagtcca agacccagat agccttccag aagtggagat
     7981 tgcacgtgaa aaaccactac cgtttaaacc atcaggtaat ggtttcggtg gtaaagctaa
     8041 aggaggtcgt ggaggccgtc gtggggacga ccgtcgagag cgtgatcgcc gtggcaatgg
     8101 tcgccgtgat gagttcaaaa aaggaagtcg tggcaacgat cgttttgata aggaaaaacg
     8161 ttaccgtaag gataataaaa aaccacgcaa tactttaagc gaaaagcaaa caggctttgt
     8221 tattcgtaac aaaggtgata aataagaaaa agcggttcaa aatgaatcgc ttttttatat
     8281 aaggagaccg aatgtgaaaa agacaaataa aatcattata ttatcttt
//
LOCUS       DAVGJJ010000083         8172 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000083 DAVGJJ010000000
VERSION     DAVGJJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.083"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            87..1295
                     /gene="sstT"
                     /locus_tag="VVE35_001693"
                     /pseudo
     CDS             87..1295
                     /gene="sstT"
                     /locus_tag="VVE35_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1363..2316
                     /locus_tag="VVE35_001694"
     CDS             1363..2316
                     /locus_tag="VVE35_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU3061845.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREKKIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2460..2840
                     /locus_tag="VVE35_001695"
     CDS             2460..2840
                     /locus_tag="VVE35_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU3061846.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLVSLQKTVEESLEAGLDFLI"
     gene            2939..3160
                     /locus_tag="VVE35_001696"
     CDS             2939..3160
                     /locus_tag="VVE35_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU3061847.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3177..3491
                     /gene="trxA"
                     /locus_tag="VVE35_001697"
     CDS             3177..3491
                     /gene="trxA"
                     /locus_tag="VVE35_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU3061848.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3796..4329
                     /gene="queF"
                     /locus_tag="VVE35_001698"
     CDS             3796..4329
                     /gene="queF"
                     /locus_tag="VVE35_001698"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU3061849.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITVQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGNFHENCINTIGKDLVNLLDPRYLEVWGKFTPRSGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            4341..4415
                     /locus_tag="VVE35_001699"
                     /pseudo
     CDS             4341..4415
                     /locus_tag="VVE35_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4539..5207)
                     /locus_tag="VVE35_001700"
     CDS             complement(4539..5207)
                     /locus_tag="VVE35_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU3061850.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(5318..6109)
                     /locus_tag="VVE35_001701"
     CDS             complement(5318..6109)
                     /locus_tag="VVE35_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU3061851.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEGVSSDGGLDKEIDKVGLPTLERSFRALVYANLLSVDANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLTEAIQSFQY"
     gene            complement(6534..7298)
                     /locus_tag="VVE35_001702"
     CDS             complement(6534..7298)
                     /locus_tag="VVE35_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061852.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGILYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(7291..8061)
                     /locus_tag="VVE35_001703"
     CDS             complement(7291..8061)
                     /locus_tag="VVE35_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061853.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIQEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT         2561 a         1797 c         1479 g         2335 t
ORIGIN      
        1 aaatattcaa aaaatagaca gaaaattctt gattttgtct atcatttata ctataatcat
       61 aatcactaca caaaaaggag attattatga aaagaatcat tcatgcttgg aataaggcaa
      121 gccttgtcaa gcgtatcttg attggtatgc tttatgggga atcctaggac tgacccttcc
      181 taatctctca gggattgggc tactcgggga tttattcgtt ggaggtctaa aagctgttgc
      241 tcctatccta gtctttgccc tcgttgccaa tgccctttcc caacatcaaa agggacaaga
      301 tagcaatatg aaaactgttg ttttcttgta tatcctatga tagggacttt cgccgctgct
      361 cttgtagctg tactagcaag tttcatcgtc cctattgaaa ttaccctaaa tagtgccaat
      421 actgaaattg caccaccaga tgggattggg caggtcctca gcaacctctt gctcaaactg
      481 gttgacagcc cagtcaactc cctgtttact gctaactata ttggaatctt atcttgggca
      541 gtcatttttg gaatcgctat gagagaagcc agtaaaaata gtaaagaatt gctaaaaact
      601 atcgctgacg tgacttctaa aattgtcgaa tggatcatca atctggctcc atttggaatc
      661 cttggtcttg tttttaaaac catttctgac aagggagtcg gaagccttgc caactacggt
      721 attttattgg ttctattagt aacgactatg ctttttgttg cccttgtggt caccctttga
      781 ttgccttctt ctttatgaga cgtaatcctt accctctagt ttggaactgc ctccgtgtca
      841 gcggtgtgac agcctttttc actcgtagtt ctgcggctaa cattcctgtc aacatgaaac
      901 tctgccatga ccttggactc aacccagata cctattctgt ttctatccca ctcggttcta
      961 ctatcaatat ggctggagta gcgattacca ttaacgtttt gacccttgct gcagttaaca
     1021 ctcttggaat tcctgttgac tttgccacag cctttgtcct cagtgtggta gcagctatct
     1081 cagcctgtgg tgcttcaggt attgccggag gttccctcct tcttatccca gttgcttgta
     1141 gccttttcgg tatttctaac gatattgcca tacaagttgt tggggttggt tttgtgattg
     1201 gtgtcatcca agactcatgt gaaacagccc ttaactcttc tacagatgtc ctctttaccg
     1261 ccgttgccga atacgcagca acccgtaaaa aataactcat caaggcaagc ctgctcatgt
     1321 cttgtctttt acgcttttat tctaacttat taggaaattc ttatgtctat tagccaacgt
     1381 acgaccaagc tcatcttagc tacctgtctt gcctgcctgc ttgcttattt tctcaatctt
     1441 tcgtcagcag tttcggctgg aattatcgct ctcttgagcc tatctgatac gcgtagaagt
     1501 actttaaaac tggctcgcaa tcgtcttttt tctatgcttc tagctctggc tatcggtgtt
     1561 ctagcttttc acttgagcgg atttcatatc tggagtctcg gcctctatct ggccttctac
     1621 gttcctttag cctacaagat gggctgggaa attggcatca caccaagcac tgttttggtt
     1681 agccatctct tggttcaaga gtcaacctct ccagaccttc tagtcaatga attccttctc
     1741 tttgctatcg gtacaggatt tgctttactg gtcaatctct acatgccttc acgagaaaag
     1801 aaaatccagc actaccacac gctggtggaa gaaaagttaa aagatatcct ccagcgcttc
     1861 aaatactatt tatccagagg agacggacgc aaccgagcac agctggtagc agaattagac
     1921 acgcttttga aagaagccct cagactggtc tatttggatc actctgacca cctctttcac
     1981 cagaccgact accatatcca ctactttgag atgagacaac gacaaagtcg tatcctgaga
     2041 aacatggccc aacagattaa cacttgtcac cttgccgcca gtgaaagcct gatcttagcg
     2101 caactctttt caaaaattgc aggtcaactg agccagacca atcctgcttc tgatttgcta
     2161 gatgaaattg aacgttatct ggaagtcttc cggaaccgca gtctgcccaa aacaagagaa
     2221 gagtttgaaa cccgcgccac tcttcttcaa ctcctacgtg aagccaaaac cttcatccaa
     2281 gtaaaagttg atttttacca aaaatataga cagtaaaaaa gaaaaccact aaaaaacaga
     2341 gcttgccagc attcttgaaa agtagtataa ttattgcatg cgcaatcatc ttgtgacgca
     2401 gagactgccc gttaatggac tttcttctat tttatgactc taaaaagaaa ggagacacta
     2461 tgacctattt ggaaaaatgg tttgacttca atcggcgtca gaaagaaatt gaaagtctct
     2521 tggaagagac cattgcccaa cagagcgagc aaagtctgac cttgaaagag ttctacctgc
     2581 tctactatct ggacttggct gaagaaaaat ctctacgcca gattgacctg ccagataagc
     2641 ttcatctgag cccgagcgca gtttctagga tggtggctcg cttggaagct aaaaattgcg
     2701 gtctactcag tcgcatgtgt tgccatcaag atagacgctc tagttttatc tgcctgacaa
     2761 atgatgggca aaagacactg gtctcgctac aaaagactgt cgaagaaagc ttggaagcag
     2821 gtttggattt cttgatttaa gatgatttta gaaaaagttg cgtgcgcaat catttttctt
     2881 gacattctct tttataagga gtaaaataaa gttagcatta aacaaaggag ttttaaatat
     2941 gattgaaatt acctatatag atgccagcaa gaacgaaaga actgtgactt tcgagtctta
     3001 tgaagacttt gaacgttcgc aacaagcttg ccttatcggc gtcgcagact actaccctgt
     3061 ccaaaaatta acttacaagg gtcataattt ggactaccat gggacttatg gagatatctt
     3121 cttctatctc atgaaacaag atttaagcca atataactaa aaaaaggaga aatacaatgg
     3181 caaaagcaat tacagatgca acattcgaac aagaaacaaa agacggtttg gtcttagtag
     3241 acttctgggc aacttggtgt ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt
     3301 ctgaagaact ttcagaagat gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa
     3361 acacagctcg tgcttttgga atcatgtcta ttccaactct tctcttcaaa aaagacggcc
     3421 aagttgtcaa acaagttgca ggtgttcaca cagcagaaca aatcaaggcc atcattgctg
     3481 aattgagcta atcaaacgag agaccaagta ctttctttgg tctctttttt cttgcccttt
     3541 gctatttttc aaaaaatatg ctagactata gtatattgaa actagaatag tacgcctcta
     3601 cttctaaaac attgttagaa atcgatttaa ctgtcctgat cgatttgtcc tgttcttatt
     3661 tcattttact atataagtag aatattacga tgtttgggat atgtcatcgc taaaaaaact
     3721 cgatttggta ttttttagct ccctaatggg agctttttgc gtgctctgaa catttcccat
     3781 tttagaagga gtactatgaa acgtcaatca gccttggtcg tctttagtgg tggtcaagat
     3841 tcaacaacct gccttttttg ggtcatgcaa cactatgaaa cagtcgaagc tgtcaccttt
     3901 gcctacggcc aacgtcatca cctcgaaatt caaattacta gagaaatcgc taaggaacag
     3961 ggcattcgtc accatatcct cgatatgtct ctgctgggac aaatcactgt tcaaccagac
     4021 tttgcgacga ttcatatttc ctacattcct gacaagctct gtgtcgagtc aaaatccctc
     4081 aaactatatc tatttagcta ccgaaaccac ggaaatttcc acgaaaactg tatcaacacc
     4141 atcgggaaag acttggtcaa cttgctagac cctcgctatt tagaagtctg gggaaaattc
     4201 actccgcgca gtggcatttc aatcgacccc tactacaact acggtaagca aggaactaag
     4261 tatgagggct tggcagaaca acgcctcttc caacacgacc tttatccaga gaaaattgac
     4321 aaccgctaaa ctcatactca atgaaaatca aagagcaaac taggaagtta gccgcaggtt
     4381 gctcaaaaca gtgttttgag gttgtagatg aaactgacga agtcagtaac catacctacg
     4441 gcaaggtgaa gctgacgtgg tttgaaaaga ttttcgaaga gtatcatacg aaaaagccct
     4501 gttcctcaag atgaggagca aggctttttg agtttcaatt attcttctgt tccaaggaaa
     4561 ttttttgcaa caagggctgc aagaactcca ccagcgattg gtgcaaggat gaaaatccaa
     4621 acttgttgaa gggctgcgcc gcctaccaag acagctggtg ccaagctacg agctgggttt
     4681 actgaaagtc cagtaatctt caatccgaca agaatcatcg ccatcaatga caaaccgatt
     4741 accaaaccag caatcgcgcc attgccctta ctttctgaag tcacagtcat gataactaag
     4801 acaaacaaga aagttgcgat gacttcaaac aagaaaccac caaagacagt gacaccgttt
     4861 gccaaggcat tttcaccaag actagcagtt gacatacctg aattacccaa gaggaagaag
     4921 actgcgccag aagcgatgaa agctccaaca acctgaccaa ggatgtagtt tacaagttct
     4981 gaagatgaca aacgtttgtt tacaaacata gcaatcgaaa cagccgggtt caagtgagca
     5041 cctgaaacag ttccgattga gtaggctgcc accacgattg ccaaaccaaa ggcaaaggcg
     5101 attccaaggt gaccaaggcc atcaaaacca tttccaaaaa caacagctcc tgtcccaacg
     5161 aacacaagca tgaacgtacc gattaactca gcaacaaatt ttttcatttt ctttctcctt
     5221 ttttcaaaaa ctagatacta gtctatcaaa agtaggaaag ggtttcaaga aaattgattg
     5281 gaaatttttt gaaaatcata gaactattag ctaatcccta gtattgaaaa gactggatag
     5341 cttctgtcag gtcatcttgt aaactatttc tctggtcaag ttggacatag acttccacca
     5401 gacaggatct aaagttagaa aatttgtaaa aatcctccct ttcttctatc ggaaaatcaa
     5461 cagtttttat ccaagaagct acttgttctt gctccaactt cccttgtaaa ataggttcat
     5521 agatcactct tgctaaacgc caatcctcat catctgtaaa gcgaatcgac attcttttaa
     5581 atagttggcc aagtatgtca aatacttcat gaactctgtt tttaggaaag tctggatgac
     5641 aaaccacctc tgtcagtaaa tcggctccat gtgcaaaaga atgaacccaa ccatactgac
     5701 ttgagaaacc tgttgtatcc ttttcttttg aaagatagtg caagccttga tttaaaagga
     5761 cattacgaat ttctgattgt aatccctgat aaaaaaccga ttgctggtta gcatctacag
     5821 acaagagatt tgcataaaca agtgccctaa aagaacgttc aagtgttggc agacctacct
     5881 tatcaatctc tttatctagt cctccatcag atgaaacccc ttcagcaatg aaatgaaatt
     5941 gctcctgtgt aaatagctct tcctgaatct ccttagcaaa gcttgtaaaa acaagatcat
     6001 cacgaatttc tggagaagga tctcccaaat gatcaagcaa ccactggatt tcctcctggt
     6061 tataatttgg tttttcttct gctatttttc ttagtaaatc ttgatacatg gtcaatacct
     6121 ctacatttct agcaactgtt caaaaaggca gtcttaaatg accttaccaa tttgtaaaat
     6181 taagttaaaa agaaaaagac atttatattc agagaaacag catatttctg aaaagatata
     6241 taaatgattt attccatatt acaacaaata aacttgtaat gatagagcct tatcacaaaa
     6301 atcacattcc tgtcccaaaa attgcgaaaa actagttata ataaagtgaa ataaaaacta
     6361 tacaaattga ttagggaatt taaaacaatt tctaacaata ttttaaaaac tgaaccgtac
     6421 tatttcaact tccataaatt acaatattga taggcacagt tcccatacct gtccaaattc
     6481 gaataacgtt tagtataaat tagaatatga ctacaacatc acaaataatt agactatcta
     6541 taatatctac gatgtaacat atacatagga agcaccgtta tcaagagcgc caaggctatc
     6601 aaccagataa gatgaagata caaacctgtc acctcacctc cccatataat acgaatcatg
     6661 cgggtagcgt gatacaaggg agaaaaccaa gtcaaatcac caagtattgg gggcaaggaa
     6721 gtaatgggaa agaaggtatc tgaaaacata taaagaggac ggattaccaa aacattgtag
     6781 taattcagaa attccctaga cggcgccaag taggtaaaga ttagtcccaa aacaccaaac
     6841 aaataaccca ctataaataa aatcagagga atcaataaca tcaagacatg aaaatgcgct
     6901 gtgataagca tggaaatcag ccaaaaaatc ccaccgaaaa tcactgcacg aatacctgcc
     6961 cagatagcct gacccaccac aatatcattc gtattgattg gcgtcatcaa catagcatca
     7021 taagtctttt caatcttata ctggataaag gcattgaccg ttgtttctgc agtggccgca
     7081 gacatggcag tcgcagcaat taaacctgta atgacaaaat cacggtagag tataccgtta
     7141 acatgcgtca aataagcacc aactccaaat cccattgcta aaagataaaa gagggggtcg
     7201 ataaggttgg ggacaattga aattttaaat aaatttctaa aactcatgta gttgcgtacc
     7261 acaacgtgtc cgatggagtg aaatgataac ttaaacatgc aagtcctcct tctctgtccc
     7321 agtcaacccc aaaaaaagcg tctcaaggtt gtcagcatca tacttgatga gcaagtcttc
     7381 tacagttcct tcttcttgga tagtcccttg gtggagcatc agcaccctat ctgtcaaata
     7441 ttcaatctca ttcatatagt gggaagtaat aatgatggaa acacctaatt ccttcaactc
     7501 caaaagttta tcccaaataa tattgcggga ttgaatgtct aaaccaactg tcggttcatc
     7561 taaaatgata atatcaggcc gattgatgag agctcttgcc aagacaagac gtcgtcgcat
     7621 cccacccgac agctgattca ccatcttttg cgcatgtttg ctcagttgaa caaaatctaa
     7681 gagagaatcg attctcttcc taagttcgac agatgagata ccatagcata gaccgtgagc
     7741 aaaaagatta tcataaacat tcaaggtcat gtccagcata tcctcctgcc ctacaatacc
     7801 cagacaagat ttactcttac gacgattggt ttcaatattt tgccccaaga tatcaatctc
     7861 tcctgaggaa attgaggtca gtgagtaaat catctttaaa gtggatgttt tgccagcacc
     7921 gttgattccc aagataccca aacactctcc ttctcccaat tcaaagctaa ttcccttgac
     7981 agcttcataa gaaccatatt gcttttttag atttttaaca cttaacttct tcactctttc
     8041 tttcccccac aaatagagca tgttggatac tttgttaatt catctcactt ttgaaaaaat
     8101 gttgagaatg taatcttaag attaacgtcc ttcaactgtg gatactgccg caattacttt
     8161 tgtaatttta cc
//
LOCUS       DAVGJJ010000084         7904 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000084 DAVGJJ010000000
VERSION     DAVGJJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.084"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..296
                     /locus_tag="VVE35_001704"
     CDS             <1..296
                     /locus_tag="VVE35_001704"
                     /inference="COORDINATES: protein motif:HMM:NF016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU3061854.1"
                     /translation="PTLFFSNRRLLFLANSLISSTTKADFIGQYDQKSNKRAIMPSNY
                     YFPPRSAVFEGKTYSVPAKLEDYLKCFYGSDYMELPPIEKRVTHQPITLRFEK"
     gene            324..716
                     /gene="tagD"
                     /locus_tag="VVE35_001705"
     CDS             324..716
                     /gene="tagD"
                     /locus_tag="VVE35_001705"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HEU3061855.1"
                     /translation="MKRVITYGTFDLLHHGHINLLRRAKELGDYLVVVVSSDEFNLIE
                     KNKVCYFNYEHRKSLVEAIRYVDLVIPETSWEQKRSDVKEYHIDTFVMGDDWIGEFDY
                     LKEEGVEVVYLPRTKEISTTKIKKDLSM"
     gene            873..1742
                     /gene="rfbA"
                     /locus_tag="VVE35_001706"
     CDS             873..1742
                     /gene="rfbA"
                     /locus_tag="VVE35_001706"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU3061856.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            1743..2336
                     /locus_tag="VVE35_001707"
     CDS             1743..2336
                     /locus_tag="VVE35_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU3061857.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            2349..3398
                     /gene="rfbB"
                     /locus_tag="VVE35_001708"
     CDS             2349..3398
                     /gene="rfbB"
                     /locus_tag="VVE35_001708"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU3061858.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            3464..4315
                     /gene="rfbD"
                     /locus_tag="VVE35_001709"
     CDS             3464..4315
                     /gene="rfbD"
                     /locus_tag="VVE35_001709"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU3061859.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            4391..4825
                     /locus_tag="VVE35_001710"
     CDS             4391..4825
                     /locus_tag="VVE35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3061860.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLGSDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            4901..5347
                     /locus_tag="VVE35_001711"
                     /pseudo
     CDS             4901..5347
                     /locus_tag="VVE35_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5418..5576
                     /locus_tag="VVE35_001712"
     CDS             5418..5576
                     /locus_tag="VVE35_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU3061861.1"
                     /translation="MSLLETAKRHQLNSEKYLFYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            5775..7757
                     /locus_tag="VVE35_001713"
     CDS             5775..7757
                     /locus_tag="VVE35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU3061862.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         2548 a         1369 c         1732 g         2255 t
ORIGIN      
        1 ttcctaccct gtttttttca aataggagat tactattctt agcgaatagt ttaatttcat
       61 caacgactaa agctgatttt attggtcaat atgatcaaaa aagtaacaaa agagctatca
      121 tgccttcaaa ctattatttt ccaccaaggt ccgctgtgtt tgaaggaaag acctatagtg
      181 ttcctgcgaa attagaagat tatttaaaat gtttttatgg ttcggattat atggaattac
      241 caccaataga aaaaagagtc actcatcaac caataacact tagattcgaa aaataatttg
      301 ctattctgta gaaggagaat ataatgaaaa gagtaattac atatggaacg tttgatttac
      361 ttcatcatgg acatatcaat ttattgcgac gtgcaaaaga attaggtgac tatctagtgg
      421 tagttgtttc aagtgatgaa ttcaacctaa ttgaaaaaaa taaagtttgt tatttcaatt
      481 atgagcatag aaaaagtcta gttgaagcta ttagatatgt tgaccttgtt attcctgaaa
      541 cgagttggga acaaaaacgc agtgatgtca aagagtatca tattgatact tttgtgatgg
      601 gggatgattg gattggagaa tttgattatc taaaagaaga aggagttgaa gtcgtttact
      661 tacctcggac gaaagaaatt tcgacaacta aaataaaaaa agatttatca atgtaatatt
      721 cgtaaataag cattgttctg ttattgttct cgaatagacc actgtatttt taagtagaaa
      781 tgctaaaatc gatagaaaag agaaaaatga taggacaata ccaatatagg taaaaaatat
      841 cgtaattaga taaataagaa aggtacccta ctatgaaagg tattattcta gcaggtggtt
      901 cgggaactcg cttgtatcct ttgactcgag ccgcatcaaa acaacttatg ccggtttatg
      961 ataaaccgat gatttactac ccactttcaa cattaatgtt ggctgggatt agggatattt
     1021 tgattatctc aactcctcaa gatttgcctc gttttaaaga gcttcttcaa gatggttccg
     1081 agtttggtat taaactttct tatgctgagc aaccaagtcc agatggtttg gcacaagcct
     1141 ttatcattgg ggaagagttt atttctgatg atagcgttgc gctaatctta ggtgataata
     1201 tctaccatgg ttctgggctt tccaagatgc tacaaaaggc agcgagtaag gagtcgggag
     1261 caactgtttt tggctaccat gtcaaggatc cagagcgctt tggtgtggtt gagtttgatc
     1321 aggatatgaa ggctatttct attgaagaaa agccagagca acctcgttca aactatgcag
     1381 ttacaggtct ctatttctat gataatgatg tagtagagat tgccaagagt attaaaccaa
     1441 gtcctcgtgg tgaactggaa attacagatg taaacaaagc ttacctagat cgtggtgatt
     1501 tgtctgttga gcttatggga cgtggctttg cttggctgga tactggaact catgaaagtt
     1561 tactagaggc ttcacagtac atcgaaacag tccaacggat gcaaaatgtt caggtagcaa
     1621 acttagaaga aattgcctat cgcatgggtt atatcagtcg tgaagatgta ttgaccttag
     1681 ctcaaccact taagaaaaat gaatacggac agtatctgct ccgtttgatt ggagaagcat
     1741 agatgacaga taattttttc ggtaagacgc ttgcggcacg caaggttgaa gctattccag
     1801 gcatgttgga gtttgatatc cccgttcatg gagataatcg tggctggttt aaagaaaatt
     1861 tccaaaagga aaaaatgctt ccacttggat ttccagagtc tttctttgca gaaggaaaat
     1921 tgcaaaacaa tgtatccttc tcacgtaaaa atgtccttcg agggctccac gcagagcctt
     1981 gggataagta catctctgta gcagatggag ggaaagttct gggttcttgg gttgatctac
     2041 gcgagggtga aacctttggg aatacctatc agacagtaat tgatgcaagc aagggaatct
     2101 ttgttcctcg aggcgtagct aatggcttcc aagttctatc agatacagtg tcatatagct
     2161 atctggtcaa tgattactgg gctctcgagc tcaaacccaa gtatgccttt gtgaactacg
     2221 ctgatccaag ccttggtatt gaatgggaaa atattgcaga agcagaggtt tcagaagcag
     2281 ataaacatca tcccctactt aaggatgtaa aacctttgaa aaaagaagat ttgtaaaaag
     2341 gaaagaatat gactgaatac aaaaatatta tcgtgacagg tggagctggc tttatcggtt
     2401 ctaactttgt ccattatgtt tacgagaact ttccaggtgt tcacgtgaca gtcctagata
     2461 agttgactta tgctggaaat cgcgcgaata ttgaggaaat tttaggtaat cgtgttgagt
     2521 tagttgttgg tgacattgct gatgcggagt tggtagacaa gttggctgct caagcagatg
     2581 ctatcgttca ttatgcagcg gaaagccaca atgataattc gctcaatgat ccatcgccat
     2641 ttattcatac taacttcatc ggaacctata ctcttttaga agctgctcgt aagtatgata
     2701 ttcgcttcca ccatgtatcg acagatgaag tttatgggga tctcccttta cgcgaagatt
     2761 tgccaggtca tggtgaagga ccaggtgaga aatttactgc tgaaacaaaa tacaacccaa
     2821 gctctccgta ctcatcaacc aaggcagcct cagatttgat tgtcaaagcc tgggtgcgtt
     2881 cttttggagt caaggcaacg atttccaact gttcaaataa ctacggtcct tatcaacata
     2941 tcgaaaaatt catcccacgt cagattacta acatcctaag tggtatcaag ccaaaacttt
     3001 acggtgaagg taagaacgtt cgtgattgga ttcataccaa tgaccattct tcaggagttt
     3061 ggacaatctt gacaaaaggg caaatcggtg aaacctactt gattggggct gatggtgaga
     3121 agaacaataa ggaagttttg gaacttatcc ttaaggaaat gggacaagct acggatgcct
     3181 atgatcatgt gactgaccgt gcaggacatg accttcgcta tgcgattgat gccagcaagc
     3241 tccgtgatga gttggggtgg aaacctgaat ttaccaactt tgaagctggg ctcaaggcaa
     3301 caatcaagtg gtatacagat aaccaagaat ggtggaaagc agaaaaagaa gctgttgaag
     3361 ccaattatgc taagactcag gagattatta cagtataaaa agcaggaaat agctgctttt
     3421 tattgctata ttgggaagag ttacatatta gaaaggtcta gagatgattt taattacagg
     3481 ggcaaatggc caattaggaa cggaacttcg ctatttattg gatgaacgta atgaagaata
     3541 cgtggcagta gatgtggcta agatggacat taccaatgaa gaaatggttg agaaagtttt
     3601 tgaagaggtg aaaccgactt tagtctacca ttgtgcagcc tacaccgctg ttgatgcagc
     3661 agaggatgaa ggaaaagagt tggacttcgc catcaatgtg acggggacaa aaaatgtcgc
     3721 aaaagcatct gaaaagcatg gtgcaactct agtttatatt tctacggact atgtctttga
     3781 cggtaagaaa ccagttggac aagagtggga agttgatgac cgaccagatc cacagacaga
     3841 atatggacgc actaagcgta tgggggaaga gttagttgag aagcatgtgt ctaatttcta
     3901 tattatccgt actgcctggg tatttggaaa ttatggcaaa aacttcgttt ttaccatgca
     3961 aaatcttgcg aaaactcata agactttaac agttgtaaat gaccagtacg gtcgtccgac
     4021 ttggactcgt accttggctg agttcatgac ctacctagct gaaaatcgta aggaatttgg
     4081 ttattatcat ttgtcaaatg atgcgacaga agacacaaca tggtatgatt ttgcagttga
     4141 aattttgaaa gatacagatg tcgaagtcaa gccagtagat tccagtcaat ttccagccaa
     4201 agctaaacgt ccgctaaact caacgatgag cctggccaaa gccaaagcta ctggatttgt
     4261 tattccaact tggcaagatg cattgcaaga attttacaaa caagaagtga gataagtagt
     4321 agaatgattt tctagtctaa taaaagaggc agataatgaa ctccaaagga gcttaagatg
     4381 tacgattatc ttgttgttgg tgctggtctc tttggcgcat agctttggct cagtttctat
     4441 tatcgctcac accatccatc agaagtttaa tctgaaggta cccaattatc gtcaagaaga
     4501 agattgggct aggatgggtt taccaatcac acgtaaggaa atctctaatt ggcatatcaa
     4561 ggcaagtcaa tactatttag agtcccttta taacctttta cgagaaaagt tgttagaaca
     4621 acctcttctt catgcggatg aaacctctta tcgggttcta gggagtgata gccatctgac
     4681 ctactattgg acctttttgt ctgggaaagc tgagaatcaa gcaatcacgc tgtaccatca
     4741 tgatcagcgt cggagtggtt cggtagtgca agaattccta ggagattatt ctggctatgt
     4801 gcattgtgat atgttgcggc agtaacttag gactttagtc ctctagttct gcctatgcga
     4861 tagcagtcca aggtttagga gcaaggcgac gctaagcttg gtaaactgcg aaccgctaga
     4921 agcttatcgt caactggaag aagctgaact tgttggatgt tgggcacatg tgagaaggaa
     4981 gttttttgaa gcgaccccca agcaagcaga taaatcatcc ttaggagcta aaggtttagc
     5041 ttattgtgat cagttatttt ccctggaaag agactgggag gctttgccag ctgatgaacg
     5101 actacagaaa cgtcaagaac atctccagcc cttaatggaa gacttctttg cttagtgccg
     5161 gcgtcagtca gttttagcag gttcaaaact aggaagggca attgaataca gcctcaagta
     5221 tgaagaaacc tttaagacta ttttgaaaga cggacatctg gtcctttcca ataatctagc
     5281 tgaacgcgcc attaaatcat tggttatggg acggagtaaa agagttcagt ggactctttt
     5341 agcctaagct aaattttaaa aagcgagggt ggttattttc tcaaagtttt gaaggagcta
     5401 aagcaacagc tattattatg agtttgttgg aaacagctaa acgtcatcaa ttaaatagcg
     5461 agaaatatct attctatctt ctagaatgtc ttccaaacga ggaaactctc gtaaacaaag
     5521 aggttttaga ggcttattta ccatggacta aagttgtaca agaaaagtgc aaataagaaa
     5581 tctccagatt aggaactatc cgtgagttct ctagtctgga gatttttcaa tatacttcgt
     5641 tattggacgg ttacgatatt catatttttt gcaaagatgt tgtttgaaaa ataattttca
     5701 aaaattctga aaattctgtt gacaactttc tgaaaagagt ctataatgga gagaaagttt
     5761 taaaggagaa aatgatgaaa agttcaaaac tatttgccct tgcgggcgtg acattattgg
     5821 cggcgactac tttagctgca tgctctggat caggttcaag cactaaaggt gagaagacat
     5881 tctcatacat ttatgagaca gaccctgata acctcaacta tttgacaact gctaaggctg
     5941 cgacagcaaa tattaccagt aacgtggttg atggtttgct agaaaatgat cgctacggga
     6001 actttgtgcc gtctatggct gaggattggt ctgtatccaa ggatggattg acttacactt
     6061 atactatccg taaggatgca aaatggtata cttctgaagg tgaagaatac gcggcagtca
     6121 aagctcaaga ctttgtaaca ggattaaaat atgctgctga taaaaaatca gatgctcttt
     6181 accttgttca agaatcaatc aaagggttgg atgcctatgt aaaaggggaa atcaaagatt
     6241 tctcacaagt aggaattaag gctctggatg aacagacagt tcagtacact ttgaacaaac
     6301 cagaaagctt ctggaattct aagacaacca tgggtgtgct tgcgccagtt aatgaagagt
     6361 ttttgaattc aaaaggagat gattttgcca aagctacgga tccaagtagt ctcttgtata
     6421 acggtcctta tttgttgaaa tccattgtga ccaaatcctc tgttgaattt gcgaaaaatc
     6481 cgaactactg ggataaggac aatgtgcatg ttgacaaagt taaattgtca ttctgggatg
     6541 gtcaagatac cagcaaacct gcagaaaact ttaaagatgg tagccttaca gcagctcgtc
     6601 tctatccaac aagtgcaagt ttcgcagagc ttgagaagag tatgaaggac aatattgtct
     6661 atactcaaca agactctatt acgtatctag ttggtacaaa tattgaccgt cagtcctata
     6721 aatacacatc taagaccagc gacgaacaaa aggcatcgac taaaaaggct ctcttaaaca
     6781 aggatttccg tcaggctatt gcctttggat ttgaccgtac agcctatgcc tctcagttga
     6841 atggacaaac tggagcaagt aaaatcttgc gtaatctctt tgtgccacca acatttgttc
     6901 aagcagatgg taaaaacttt ggcgatatgg tcaaagagaa attggtcact tatggggatg
     6961 aatggaagga tgttaatctt gcagattctc aggatggtct ttacaatcca gaaaaagcca
     7021 aggctgaatt tgctaaagct aaatcagcct tacaagcaga aggagtccaa ttcccaattc
     7081 atttggatat gccagttgac cagacagcaa ctacaaaagt tcagcgcgtc caatctatga
     7141 aacaatcctt ggaagcaact ttaggagctg ataatgtcat tattgatatt caacaactac
     7201 aaaaagacga agtaaacaat attacatatt ttgctgaaaa tgctgctggc gaagactggg
     7261 atttatcaga taatgtcggt tggggtccag actttgccga tccatcaacc taccttgata
     7321 ttatcaaacc ttctgtagga gaaagtacta aaacatattt agggtttgac tcaggggaag
     7381 ataatgtagc tgctaaaaaa gtaggtctat atgactacga aaaattggtt actgaggctg
     7441 gtgatgagac tacagatgtt gctaaacgct atgataaata cgctgcagcc caagcttggt
     7501 tgacagatag tgctttgatt attccaacta catctcgtac agggcgtcca atcttgtcta
     7561 agatggtacc atttacaata ccatttgcat tgtcaggaaa taaaggtaca agtgaaccag
     7621 tcttgtataa atacttggaa cttcaagaca aggcagtcac tgtagatgaa taccaaaaag
     7681 ctcaggaaaa atggatgaaa gaaaaagaag agtctaataa aaaggctcaa gaagatctcg
     7741 caaaacatgt gaaataactg ttgcaaaata taagaaagga tttagtattt cccttgaatg
     7801 ctgaatcctt ttttacattt gtaaagaaag attctaaatg tacggacccc caaaagttgg
     7861 agcctctttt tgtcagaata gagaaaattt ttgttaattt tact
//
LOCUS       DAVGJJ010000085         7815 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000085 DAVGJJ010000000
VERSION     DAVGJJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.085"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            88..777
                     /gene="coaB"
                     /locus_tag="VVE35_001714"
     CDS             88..777
                     /gene="coaB"
                     /locus_tag="VVE35_001714"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU3061863.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            789..1340
                     /gene="coaC"
                     /locus_tag="VVE35_001715"
     CDS             789..1340
                     /gene="coaC"
                     /locus_tag="VVE35_001715"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU3061864.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1324..1887
                     /locus_tag="VVE35_001716"
     CDS             1324..1887
                     /locus_tag="VVE35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3061865.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2169..2696
                     /locus_tag="VVE35_001717"
     CDS             2169..2696
                     /locus_tag="VVE35_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU3061866.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2708..3223
                     /locus_tag="VVE35_001718"
     CDS             2708..3223
                     /locus_tag="VVE35_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU3061867.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3220..3687)
                     /locus_tag="VVE35_001719"
     CDS             complement(3220..3687)
                     /locus_tag="VVE35_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU3061868.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(3761..4249)
                     /locus_tag="VVE35_001720"
     CDS             complement(3761..4249)
                     /locus_tag="VVE35_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061869.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(4215..4778)
                     /locus_tag="VVE35_001721"
     CDS             complement(4215..4778)
                     /locus_tag="VVE35_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061870.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIEYGILREDWEKINDGYYQINGNS"
     gene            complement(4788..6776)
                     /gene="uvrB"
                     /locus_tag="VVE35_001722"
     CDS             complement(4788..6776)
                     /gene="uvrB"
                     /locus_tag="VVE35_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU3061871.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6853..7806)
                     /locus_tag="VVE35_001723"
     CDS             complement(6853..7806)
                     /locus_tag="VVE35_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3061872.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2342 a         1747 c         1378 g         2348 t
ORIGIN      
        1 agtttgaaac tatagtaaat cttcgttttt accaaaaatt tatcactttc attttactta
       61 ccgcttattt ttgtgtacaa tagtgctatg aaaattttag ttacatcggg cggtaccagt
      121 gaagctatcg atagcgtccg ctctatcact aaccattcta caggtcactt ggggaaaatt
      181 atcacagaga ctttgctttc tgcagggcat gaagtttgtt taattacgac aaaacgagct
      241 ctgaagccag agcctcatcc taacctaagt attcgagaaa ttaccaatac caaggacctt
      301 ctaatagaaa tgcaagaacg tgttcaggat tatcaggtct tgatccactc aatggctgtt
      361 tctgactaca ctcctgttta tatgacaggg cttgaggaag ttcaggctag ctccaatcta
      421 aaagaatttt taagcaagca gaatcatcag gccaagattt cttcaactga tgaggttcag
      481 gttttgttcc ttaaaaagac acccaaaatc atatccctag tcaaggaatg gaatcctact
      541 attcatctga ttggtttcaa actgctggtt gatgttaccg aagatcatct ggttgacatt
      601 gcacgaaaaa gtcttatcaa gaatcaagca gatttaatca tcgcgaatga cctgactcaa
      661 atttcagcag gtcagcaccg agctatattt gttgagaaaa atcagcttca aacagtccag
      721 actaaagaag aaattgcaga actcctcctt gaaaaaattc aagcctatca ttcttagaaa
      781 ggaaaactat ggcaaacatt ctcttggctg taacgggttc aatcgcctct tataagtcgg
      841 cagatttagt cagttctcta aaaaaacaag gccatcaagt cactgtctta atgactcagg
      901 ctgctacaga gtttatccaa cctttgacac tacaggtact ctcacagaat cctgtccact
      961 tggatgtcat gaaggaaccc tatcctgatc aggtcaatca tatcgaactt ggaaaaaaag
     1021 cagatttatt tatcgtggta cctgcaactg ctaacactat tgcaaaacta gctcacggat
     1081 ttgcggacaa catggtaacc agtacagctc tagccctacc aagtcatatt cccaaactaa
     1141 tagctcctgc tatgaataca aaaatgtatg accatccagt aactcagaat aatctgaaaa
     1201 cattagaaac ctacggctat cagctgattg ctcctaagga atctctacta gcttgtggag
     1261 accacggacg aggagcttta gctgacctca caattatttt agaaagaata aaggaaacta
     1321 tcgatgaaaa aacgctctaa tattgcaccc attgctatct tttttgctac catgctcgtg
     1381 atacactttc tgagctcact tatctttaac ctttttccat ttccaatcaa accgaccatt
     1441 gttcatattc ctgtcattat tgccagcatt atttatggtc cacgagttgg ggttacactt
     1501 ggatttttga tgggattact tagcttgacg gttaacacga ttacgattct accgacaagc
     1561 tacctcttct ctcccttcgt accaaacgga aacatctact cagctatcat tgccatcgtc
     1621 ccacgtattt tgattggttt aactccttac ttagtctata aactgatgaa aaacaagact
     1681 ggtctgattt tagctggagc ccttggttcc ttgacaaata ctatctttgt ccttggagga
     1741 atcttcttcc tatttggaaa tgtttataat ggaaatatcc aacttcttct ggcaaccgtt
     1801 atctcaacaa attcaattgc tgaattggtc atttctgcaa ttctaaccct agccattgtt
     1861 ccacgactac aaaccttgaa aaaataaaaa caatctccgc tttcaaacct gaaggtggag
     1921 attttgctct atacagtttc tttttaatga aatctttact atagtaaaac gaaataagaa
     1981 caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga agtagaggtg
     2041 gactattcta gtttcaatct actataactt ttaactaaaa aatgatctat aaacccaagt
     2101 aaattcttgc tttattgtct ggtttattgt accatactag tgtatatgca gttaaaaagg
     2161 agattcttgt gaatacacgg aaaaagacac aatttatgac aatgacagcc cttttaacgg
     2221 ctattgcgat tttgattcca attgttatgc ctttcaagat tgtcattcca cctgcttcct
     2281 atactttggg gagccacatc gctattttta tagccatgtt cttgtcgccc ttgatggcag
     2341 tttttgtcat cctagcctct agttttggat ttttgatggc tggctatccc atggttatcg
     2401 tttttcgggc tttttcccat atatcttttg gtgctttagg agctctttac ctacaaaaat
     2461 tccccgatac cctagataaa ccaaaatctt cctgggtttt caattttgtt ttggctgttg
     2521 ttcatgccct tgctgaagta ttagcctgtg tcctttttta cgcaacttcg ggtaccaatg
     2581 tagaaaacat gttctatgtt ctatttgtac tagttggatt tggcacaatt atccatagta
     2641 tggtagacta tacattagca ctagctgtct ataaagtgct tcgaaaacgc cgttaaaagg
     2701 aaaagctatg acaaaagatc gcaaacaagc cctgctccaa ctcttgaaag aagcccctaa
     2761 agcccttaat ggccaaagtt tggcggaaca ttttcatgtc acacgtcagg tcattgtgca
     2821 ggacattgca attttgagag ccgatggcgc tcctatccta tccaccaatc gtggctacat
     2881 ctataagcaa attgatacca atccttatgt tcacaaactt ttcaaagtga aacatgaagt
     2941 tgaagaaatc ggtcaggaac tccttgctat tgtcgataat ggtgggcatg tccagaatac
     3001 cttgattgac catcccgttt atggagaaat tgaaaccttg cttaaactgt cttgtcgcag
     3061 agatgtgcaa cattttctag aacaagtcga gcattctgac tttagacctc tatctgaatt
     3121 gacagatggc atccattacc acctagtcga agccgaaaca caacaagacc tccactatat
     3181 cgaggaggcc ttggatcagc taggttattt agtaaaagac tagaagattt tcttttccca
     3241 atcgtcttct gtacggcgag gatagaaaaa ctctgacttg tcgggagctt ccatcatttc
     3301 catcaatggt agcatatcat aggccagatt taagtttgga atctggtctt tttgcaccca
     3361 agaaacttct ccctcttctg aagattgaag ggtaccagag aactcagtcg ccttataaca
     3421 aatgacaata tagcgcccac ctgtatctag tggccaattt ttaatgccga caagttgagg
     3481 attttggata gtcaaccctg tttcttcgta gatttcacga atgacagact ccgcaaaagc
     3541 ctcatcattt tctacatgac ctccaggaaa ggcataacca gaccagcgat tgttttcagg
     3601 ggcgcgatac tgcatcacca cgcgctgagt ttcgaggtct tcaatcagac agatatttgt
     3661 taaaattgtt aattgggaac gggacataaa tttactcgct ttctaattta ttaaattttc
     3721 agacttacta ccagagatat tatcttttac ttttttaaaa ttatttagaa tagaacacca
     3781 ttcgtttttc ctttataggc tttccaagtt caagtatttt ttcttgtcca tcaaaagtaa
     3841 aacccatttt ttgatagaaa gcaatggcgc gcttgttatc tttcaatacc cataagaaaa
     3901 tttcagaaaa atgattaaga gcagtcaaag ctgctttcat taacttttgt gcgatacctt
     3961 ttccataata gtcttttaaa acatataaag caataatttc accagcttga atagtctcat
     4021 cacgaaaatt gccataactg ataaaaccaa ccaccttcac gccatccatc gcaatcaatg
     4081 tattttctgg atacttttga ctaaagaatc gacatctttc taatgtcatt gtatcctgaa
     4141 attccgcagg caaaaggtca tcataagact ctctccacgt ttgccagtga acgagggatt
     4201 tgccttctat ctcttcagga gtttccattg atttgataat aaccgtcatt tattttctcc
     4261 cagtcttctc tcaaaatacc atattcaata ctatcaaaat atttaccttg ataataacga
     4321 acttttggaa tatgagcttc ttttttcatt cttaattttt cagcaagttt catcatacca
     4381 agatttcctg accaagttgt caaaaccaga tgctccaact ccaagtaatc ctgaaacgtc
     4441 ctatctatcc actgcaacat agcaactttc ccaataccag tgttccagaa ttttttatca
     4501 taaataccaa ttcccaattc catccatctt gtttctttac atacccaata acgcgaaaca
     4561 gtcccaacta gtttatcatc aacaaaaata ccaaggcgat ttgagttgct taaaatggat
     4621 tctgattttt gtagttcgaa ttctttaaaa tttgaaaaat actgataatc atcataatag
     4681 ggagcatcat actgtttcca aactggatta agctgtgaat aagccatttc ccataaagac
     4741 atcaaatctt ctttctttaa ctttcttaaa taaatcatac tattccccta atccaaggcc
     4801 ttgacttcca acatcatatc acggatctga gccgccagtt caaagtcaag cacttcgacg
     4861 gcttcttgca tttgtttttc tagtttcttg acgagttctt tgcgctcttg tttgttgagg
     4921 ctattgatgt cgacttcctt gtcctcttcc ttagcaactg ccttggtcac ggcaatcagg
     4981 tcacggattt ctttcttgat tgtctgtggt acgataccat gctcttcatt ataggccatc
     5041 tgaattttcc gacggcgggc agtttcatcg atagcacgtt gcatagactg ggtaaccgta
     5101 tctgcataca tgatgacatg accttcactg ttacgggcgg cacgcccaat ggtctggatg
     5161 agtccacgct cgttacgaag gaaaccttcc ttgtcagcat cgagaatggc aaccaagcta
     5221 acctcaggaa cgtcaatccc ttcacgaagg agattgattc caaccaagac atcaaagaca
     5281 cccaagcgga gatcacggat aatctccgtc cgttccaagg tcttgatatc cgagtgcatg
     5341 tacttgacct tgatgcccat ttccttgaaa tagtcggtca agtcctctgc cattttcttg
     5401 gtcaaggtgg tgataaaggt tcgttcattc ttttcaacac gggcattgat ttcacctaag
     5461 agatcatcaa tctgtcccat agtcggacgg acttccacct caggatccaa aagccctgtc
     5521 ggtcgaatga tttgctcaat caccgtctca gtctgttcat tttcatagtc accaggtgtc
     5581 gctgatacgt aaacaatctg atgcacatgg ctttcaaact cctcacgacg gagaggacga
     5641 ttgtccaagg cagacggtaa acggaaacca taatttacta gcatttcctt acgcgaacgg
     5701 tctccattgt acatgccttt tatttgtccc atagtcatat gactctcgtc aatcataatc
     5761 aaaaaatcat ctgggaaaaa gtcgagaagc gtataaggag gctcaccttc actacgtcca
     5821 tccatgtggc gagaataatt ttcaacccca tttgtatagc ccatctcgcg cagcatttcg
     5881 atatcgtact ctgtccgttg tttcaagcgc tgggcttcaa gcaatttacc ttccttctca
     5941 aagacagcta actgctcttc taattctgct tgaatcttgg caacggcaac ttccatatgg
     6001 tcgtcattgg tcacaaagtg agtagctggg aaaatcgcca aatgatccac ctctcccaat
     6061 acctgacctg tcagagcctc aacctcacgg atacggtcaa tttcgtctcc aaaaaattct
     6121 actcgaaagg catgttcatc tcgggaagct gggaaaatct ctaccacatc cccacgaacg
     6181 cgaaatcttc cgcgttggaa atcaatatca ttacgttcaa actgaatatc gaccaagtca
     6241 ttcaagagtt tatcacgaga aatctctaga ccaggacgga gactaacgac actatcagcg
     6301 tattccttgg gcgaacccaa accatagata caagagactg aggccacgac aataacatca
     6361 ttacgctcca aaagggctga ggtagctgag tggcgaagct tgtcaatctc gtcattgaca
     6421 gaactatcct tctcaatata ggtatcgcta gaagggacat aggcctctgg ctggtaataa
     6481 tcatagtagg atacgaaata ctcaactgca ttttcaggga aaaattcctt aaactcccca
     6541 tagagctgac cagccagagt tttattgtgg gcaataacca gagttggttt attgacttta
     6601 gaaatgacct gactcatagt ataggtcttc cctgttccag tcgcccccag cagaatctga
     6661 gctttttctc ccccctcaat gttatccacc aactgctcga tagcttgggg ttgatctcct
     6721 gatggttgat attttgatac tagtttaaat tgattatctg taatgtgatt gatcataaga
     6781 atcctctctc catgtgctat tcctatttta gcatacttat agtattttca cgaagaaaag
     6841 gacggaccga agtcacatcc tttcattttc tttctttaat ttattagcaa gatagacaat
     6901 cagcagtagt aataccagac ttgtcataat ggaaccttct atgccaaaag aaccacctga
     6961 tagccaatct tgattgcctt gtggtaaaaa ggtcatcaga gacgttcctg acggttgacc
     7021 actaactaaa atcccaaaga gattaccctg agcaaaattc caagcaccat gaatacctgc
     7081 aacaccccaa actgtatcag ttttgagaag ataaagagcc atggcaactc cgaataaaaa
     7141 gagatttact agagatagag gggtgaggcc agaattgccc atatgaagca gggtaaagaa
     7201 caggctagat ataagaatag ctagttttag attggttctt gaggccaatt gaggaagtag
     7261 ccaagcacgg gccaccactt cttctgctgt cccctgtaaa atccaaaatg ggatagtaaa
     7321 gacgacaaag gcaagagaat aaggattcaa gtgaatggat tccaaacgat attgacctaa
     7381 gaccactaaa cctaacaagg tcagaagaaa aagtgccaag cctagactaa atcctttcag
     7441 aagattgctg aggaaattct ctctataaaa tcccaaggtt cgaataggtc ttttctcaac
     7501 ttttcttgtc catctgaaca cagtgttaag aataaaacca aaggccagta atcctaaaat
     7561 taaacgaaag tcacttgttt tatccgtcaa gctctgatgc aaggtctgca agtaggttgc
     7621 aagattttga ccagcctctc caaaatttct agcaagtccg atgagagcta acaagccaat
     7681 accatacaaa gtaaaagcca caaactctcc tatgaagaca aaaacaaagg ctaacaaggg
     7741 gcttgtgtaa atgtttaaac tcatttttga agcttggaag tctttaaata ttcttttgtt
     7801 cttcatatcc ccgtc
//
LOCUS       DAVGJJ010000086         7038 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000086 DAVGJJ010000000
VERSION     DAVGJJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.086"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(218..1187)
                     /locus_tag="VVE35_001724"
                     /pseudo
     CDS             complement(218..1187)
                     /locus_tag="VVE35_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1348..1719)
                     /locus_tag="VVE35_001725"
     CDS             complement(1348..1719)
                     /locus_tag="VVE35_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061873.1"
                     /translation="MIKKNHILCEIVPESVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQIMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLTIMLLLIWGLKYYKK"
     gene            complement(2187..2306)
                     /locus_tag="VVE35_001726"
     CDS             complement(2187..2306)
                     /locus_tag="VVE35_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU3061874.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(2410..2559)
                     /gene="blpO"
                     /locus_tag="VVE35_001727"
     CDS             complement(2410..2559)
                     /gene="blpO"
                     /locus_tag="VVE35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU3061875.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(2803..3006)
                     /gene="blpN"
                     /locus_tag="VVE35_001728"
     CDS             complement(2803..3006)
                     /gene="blpN"
                     /locus_tag="VVE35_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU3061876.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(3022..3276)
                     /gene="blpM"
                     /locus_tag="VVE35_001729"
     CDS             complement(3022..3276)
                     /gene="blpM"
                     /locus_tag="VVE35_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU3061877.1"
                     /translation="MDTKMMEQFHEMDIAMLSSIEGGKNNWQTNVFEGGSAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            3573..4160
                     /locus_tag="VVE35_001730"
     CDS             3573..4160
                     /locus_tag="VVE35_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU3061878.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            4087..5730
                     /locus_tag="VVE35_001731"
     CDS             4087..5730
                     /locus_tag="VVE35_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU3061879.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFKPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILKAYELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            5741..>7038
                     /locus_tag="VVE35_001732"
     CDS             5741..>7038
                     /locus_tag="VVE35_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU3061880.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIPPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVT"
BASE COUNT         2325 a         1454 c         1040 g         2219 t
ORIGIN      
        1 ttttgtcctg ttacaacttt gacaagttta gtatatcatc gtttattaat ttttttcatc
       61 caaatcttga attgtcatca aaacgtcttg aattgttaaa aaatttaaaa agcaagcatt
      121 aaaaacatac ttgccctttc actcttgtct ataggaaaat tctagccata tgttattaca
      181 aacaaataat atcattgcac ctgtttacca tctattttta tttatgaatt ctttgtctcc
      241 acttaatcat acctgaagta attaagagtg caaccgctag acgtaataga ttgtaacttt
      301 cttccactcc tgtattaggt agtaattgtt tactcaaatg actggttttg gagggagaat
      361 cattcccctc aaccttggat aaaggagttt gtaacttctc agtattttca gaaatcgtct
      421 gagatgactc ccctcgcttc ttcttatcta tcattaccaa atcttcagac tgaacagtta
      481 tagtgttaga aggaggaata ttatccttca atggctctct gttatcactg ttattagtcg
      541 aattttcctt tgaattcttc acctctcctg ttttatcatc acggacactt tcagttactg
      601 tttcctcaga agatttcact gtctccgttt gcttaggtga gtttctcgaa tacatataat
      661 catacacctg ttgtgcagtt gctccgtcac caacccatcc agatgtcgga tgtccatttc
      721 ttaaatacaa aattgttggt gttcctggaa taccgacttt tttaaaatag aaactctctc
      781 gcttccccat caaaatcctt accatccaaa tcataatact ctaacttctt ttcaatcaag
      841 ttattaaatt ctttcaattc aggagaaaat tgacgacaat gagagcacgt ttttctacca
      901 aaataaatag tatgttccaa ttgatcccct gtaaaagatt gacgaacagc ttcaatatct
      961 atctttttaa agtccgtaac attcttctca tactcttctg gggttacaac agaaggctta
     1021 tgctccacct tttccttagc cgatggcaaa tctggcgcca caagagaact atcattcgcc
     1081 tgggtagatg aagtaatagg ctgttcttct gcatatgcaa gacctgcagt aatagatgca
     1141 aatacaagtg gtacaagtaa tagcgatttt atcttttgtt tcttcataag aatactcctt
     1201 tacatttgtt atctttatct taccttattt aagattacaa attcatctaa atcttaaatg
     1261 gtcatcgaaa catcttgaat tgcaaaaact tcattataaa agggtaagta tttcaaaaac
     1321 acttacccct ttatacttta ttaagctcta ctttttataa tacttcaatc cccaaatgag
     1381 cagaagcata atggtcaagc agagaatagc accaatgtag gcaattattt gttggtagga
     1441 ttctcctgct gtgatccctc tatacaaaca aataatagac ataaaacctg tcaagccgat
     1501 gaacatgatt tgattagttc taggactaac caaatcatcg tcttcaaact cttttatccg
     1561 catttcccta gtgaggtaaa cagtgaccaa aatagaagcc aagttaataa ccactaaaag
     1621 aaattggaaa actgacgaaa tagaaataga taagtagaaa caagcaaagg caactgactc
     1681 aggaacaatc tcacaaagaa tatgattctt tttaatcaag aaaatctttc atttcttttc
     1741 tttcttttta attaaagaaa aatagatcat aaccgcaatc atataggcta tggtataaaa
     1801 tagattatac catatactct ccctaagcgg ataaagaaag atggacatga tcagatacag
     1861 aacgaaaata atcaatattt ttttcttcat aagttttcct cctaaatgtg cgatttatct
     1921 tagttgagca agaacattta cactgctagt atagcactta ttttgacctt ggatcactca
     1981 aatcataaat ggtcatcaaa acctcttgaa ttgtaaaaat taaaaaagca agcatgaaaa
     2041 acatactttc ctctttatat tgtattgata ccaacttgtt tgtagacttt tcatcctgct
     2101 atcacatatc attttgacag gcgaaacaat attaaagaaa ctcccctgta aattaagcta
     2161 gcaaatacag gggagaaatt tattttttag agagtactat ccgtatcctt tttggaagat
     2221 tttgaaaata tttttctaat taagtcatcc atataaggac caaatatacc aactactaaa
     2281 ccaataataa aacttttaaa atccataatt accaccaaca tgttgctgca taggctacac
     2341 ctccaagtat agctccacct gcagcaccag ttacacctat tcctatagca aatggtccca
     2401 atagaaatgt caaaccgttg ttgcacaccc atcaattgcg ccatatgcaa cccctgctgc
     2461 acaactaatt tttcttcccc aatcaatatc tccaccttca acgcaagcaa gcatttcatt
     2521 atccataaca gaaaattgtg acatcatttt tgtattcata tagtatgtca cttttcagtt
     2581 acggaacaag tttaatataa aaattatcaa aaaaacatag gcaataaaga gaaaaattaa
     2641 tttatcatag attagaaata atatgacaaa acaattcaat gatgttaatt caatagtctt
     2701 ttgtttttta tcagagatac ttatggatag ataaataaga taggtttgaa aagcgaagag
     2761 aataataaag aatatagcct tcataaaatt tagctttcat ttttatgatg tagcggtata
     2821 ggctaaatat ccacaaacca ctgctcctcc aattcctcct attgcagcgc cccatggtcc
     2881 tagaattttc ccatatttca ctccacccgc tgcacaacct aaagcagcaa ctacagctgc
     2941 tcctccggaa ttacctccgt aaacctcact cagcattgtt tcatttatat tacaataagt
     3001 attcatacaa gtctcctttt gttaaaatcc acccgttgcc cctgttactc ctgcccaaag
     3061 agccacacca aatttagctc ctatgtatcc acatgctccc ataaatggcg ctccaacacc
     3121 actcgcagca caaatagctg tccctaaccc ccagccacca aaagcagcac taccaccttc
     3181 gaaaacatta gtttgccaat tattcttgcc tccttcaata ctagataaca tagctatatc
     3241 catttcatga aattgttcca tcatttttgt atccatgaca aatactcctt ttttattttt
     3301 aatttttgtc ccgttgcaac tttgacaagt ttagtatatc attatttttt aatttttttc
     3361 atccaaatct tgaattgtca tcgaaacgtc ttgaattagc ttttttattt caagccacct
     3421 ctatttcaag ccacctctaa atgtttaaaa aaaataattt ctaatcactt ttttaccatt
     3481 caggaagttt taatgactat tcaagatttc ataaaatatg aacttagttt tatgacataa
     3541 tagacctatc cactatatga aaggaattgc caatgacttc ttataaacgt acatttgttc
     3601 ctcaaataga tgcgagagac tgtggtgtcg ctgccttagc ctcgattgct aaattctatg
     3661 gttcagattt ttctctagct cacttgagag aacttgcaaa gaccaataaa gaagggacga
     3721 ctgctcttgg cattgtaaaa gccgctgatg aaatgggctt tgaaacaaga cctgttcaag
     3781 cagataaaac gctctttgac atgagtgatg tcccctatcc atttatcgtt cacgttaaca
     3841 aagaaggaaa actccaacat tactatgttg tctatcaaac aaagaaagac tatctgatta
     3901 ttggtgatcc tgacccttct gtaaaaatca ctaaaatgtc aaaagaacgc tttttctatg
     3961 aatggactgg agtagctatt tttctagcta ccaaacccag ctatcaaccc cataaagata
     4021 aaaagaatgg tctactaagc aagcttcctt cctctgattt tcaaacaaaa atctctcatt
     4081 gcttacattg ttctctcaag cttattggtc actattatca atataggtgg ttcttactat
     4141 ctccaaggaa tcttggatga atacattcca aatcagatga aatcaacttt aggaatcatc
     4201 tcagttggtc tggttatcac ctatatcctc caacaagtca tgagcttctc cagagattat
     4261 ctcctaaccg ttctgagcca gagattaagc attgatgtga ttttatccta tattcgccat
     4321 atttttgaac ttcccatgtc tttctttgcg acacgtcgta caggagaaat catttcacgg
     4381 ttcacagatg ctaactctat tatagatgcc ttggcttcta ccattctttc tctttttctg
     4441 gatgtttcta ttctgattct tgtaggggga gtcttgctgg cacaaaaccc taatctcttc
     4501 ctcctttctc ttctttccat tcctatatac atgttcatca tcttttcttt tatgaaacct
     4561 tttgaaaaaa tgaaccatga tgtcatgcaa agtaattcta tggttagctc tgccattatc
     4621 gaagatatca acgggattga aactataaag tcgctcacga gtgaagaaaa tcgctatcaa
     4681 aatatagaca gcgaatttgt agattatttg gaaaaatcct ttaagctcag taaatattct
     4741 attttacaaa cgagtttaaa gcagggaaca aaattagttc tgaatatcct tatcctatgg
     4801 tttggcgctc aattagtcat gtcgagtaaa atttctatcg gtcagctgat tacctttaac
     4861 acactttttt cttactttac aactcctatg gaaaatatta tcaacctcca aaccaaactc
     4921 caatctgcga aggtcgctaa taaccgtttg aacgaagtct atctagtcga atctgaattt
     4981 caagttcaag aaaaccctgt tcattcacat tttttgatgg gcgatattga atttgatgac
     5041 ctttcttata agtatggttt tggacgagat accttaacag atattaatct cacgattaaa
     5101 caaggagata aggttagcct agttggagtt agtggttctg gtaaaacaac tttagccaaa
     5161 atgattgtca atttctttaa accctacaaa gggcatattt ccatcaatca tcaggatatt
     5221 aaaaacattg ataaaaaagt cttgcgccgt catattaatt acctacccca acaagcctat
     5281 atctttaatg gctctatttt ggaaaactta accttgggcg gtaatcatat gattagccaa
     5341 gaagatattc taaaagctta tgaattagct gaaattcgtc aagacattga aagaatgcct
     5401 atgggctatc aaactcagct ctctgatgga gctggtctat caggaggaca aaagcaacga
     5461 atcgctctcg ctcgtgctct tttaactaaa tctcctgttt taatactaga cgaagctact
     5521 agcggtcttg atgtcttgac tgagaaaaaa gttatagata atcttatgtc cctaactgat
     5581 aaaaccattc tctttgtagc ccatcgtctc agtatagccg aacgaactaa ccgtgtcatt
     5641 gttcttgacc aggggaaaat cattgaagtt ggtagtcacc aagagttaat gcaggcgcaa
     5701 ggcttctacc atcatctgtt caataaataa ggagaatgtc atgaatccta atctttttag
     5761 aagcgtcgag ttttatcaga gacgttacca taactatgcg acagtattaa tcatacctct
     5821 ttcattatta tttactttca tcttgatttt ctcccttgtt gccacaaaag aaattactgt
     5881 tacttcccaa ggggaaatcc cccctacaag tgtcattgca tctattcagt cgaccagtga
     5941 taatcctatc cttgctaatc atttagtggc aaatcaagta gttgagaaag gtgacttact
     6001 catcaaatac tctgaaacaa tggaagagag tcagaaaact gccttagaaa ctcaattaca
     6061 aagacttgag aagcaaaaag aaggacttgg aatcttgaaa caaagcttag aaaaagcgac
     6121 tgatcttttt tctagcgagg atgagtttgg ctaccataat acttttatga attttactaa
     6181 acaatcccat gatattgaac tgggtatctc aaagactaac actgaagttt caaatcaagc
     6241 taatcttacc aatagcagtt catcagccat cgaacaagaa attacaaaag ttcaacaaca
     6301 aattggagaa tatcaagagc tgagagatgc tatcgtaaat aaaagagctc gcttaccaac
     6361 tggcaatccg caccagtcaa ttttaaatcg ttatcttata gcctctcaag aacaaacaca
     6421 aggaacggca gaggagccat ttttatctca aattaatcaa agtattgcag gtctggaatc
     6481 atctatcgca agcctcaaaa ttcagcaagc tggtatcgga agtgtagcaa cttatgataa
     6541 tagtttagca accaaaattg aagtactccg cactcagttt ttacaaacag cctcacagca
     6601 acaactaacc gtggagaatc aattaacaga attaaaagta caactagatc aagcgacaca
     6661 gcgcttagaa aataatacct taaccgctcc aagtaaaggt atcgttcatc tgaacagcga
     6721 atttgaaggt aaaaatagaa ttccaactgg tacagaaatt gctcaaatat tccctgtcat
     6781 cacagataca agagaagtac taatcactta ctacgtatct tctgactatc tacctctact
     6841 agataaagga caaactgtaa gattaaaact ggagaagatt ggaaatcacg gcaccaccat
     6901 catcggccaa cttcagacaa ttgatcaaac tcctaccaga acagagcaag gaaatctctt
     6961 taaattaacc gctcttgcaa aactatctaa cgaggatagt aaactcatcc aatatggctt
     7021 acaaggtcgc gtcactag
//
LOCUS       DAVGJJ010000087         6913 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000087 DAVGJJ010000000
VERSION     DAVGJJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.087"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..499
                     /locus_tag="VVE35_001733"
                     /pseudo
     CDS             1..499
                     /locus_tag="VVE35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            560..1522
                     /locus_tag="VVE35_001734"
     CDS             560..1522
                     /locus_tag="VVE35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU3061881.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1699..2706
                     /gene="gap"
                     /locus_tag="VVE35_001735"
     CDS             1699..2706
                     /gene="gap"
                     /locus_tag="VVE35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU3061882.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHNVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2774..3610)
                     /locus_tag="VVE35_001736"
     CDS             complement(2774..3610)
                     /locus_tag="VVE35_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU3061883.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3736..5931
                     /locus_tag="VVE35_001737"
     CDS             3736..5931
                     /locus_tag="VVE35_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU3061884.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSNRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDNYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPVNTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5984..6136
                     /gene="rpmG"
                     /locus_tag="VVE35_001738"
     CDS             5984..6136
                     /gene="rpmG"
                     /locus_tag="VVE35_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU3061885.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6146..6322
                     /gene="secE"
                     /locus_tag="VVE35_001739"
     CDS             6146..6322
                     /gene="secE"
                     /locus_tag="VVE35_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU3061886.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6377..6913
                     /gene="nusG"
                     /locus_tag="VVE35_001740"
     CDS             6377..6913
                     /gene="nusG"
                     /locus_tag="VVE35_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU3061887.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2152 a         1201 c         1485 g         2075 t
ORIGIN      
        1 tagccttatt tcttaaaaat tttaatagtt taaagcacct agcacctgtt tagattgatg
       61 aaacaggatt cgatacttat ttttatcgag aatatggtcg ctcattaaaa ggtcagttaa
      121 tatgaggcaa agtatctgga agaagatatc agaggatttc tttggttgca ggtctaacaa
      181 atggtgaatt aatcgctcca atgacttacg aagagatgat gacgagcgac ttttttgaag
      241 tatggtttca gaagtttttc ttaccaacat taaccacacc atcggttatt attatggata
      301 atgcaagatt ccatagaatg ggtaagttag aactcttgtg tgaagagttt gggcataaac
      361 ttttacctct tcctccctac tcacctgagt acaatcctat tgagaaaaca tgggctcata
      421 tcaaaaagcc tcgtaaaagg tattaccaag ttgcaatacc ttttacgagg cttttttatc
      481 ctgctcttgt ttcaattgac tatagttcac ggatacagtt gggaaagaag ctattaaata
      541 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaatgga
      601 tctgagtcaa acgatgcctt cttatgctca agtagtgagt ctttatgatg atacaagtat
      661 ctctgttagt aataatgaga cggacaaagt gttggctggc tccctctata ctgatacaaa
      721 tgaacaggga ttaacgattc caagcagttt actaaaaaat tggaatgaac agacaggaaa
      781 aaatttgaca gctaatgatc ttattggcaa atcagtctca gccagcattg tagaaagtgc
      841 tgccgaaact agtaagattg ctcaatttca aacgaagatt gtacgtgtaa ttaatgatga
      901 agatgacatg gaggacagca acagtttcat gctgtctcat caaatggaaa cgattttgaa
      961 agaggctgga tttacgaaag ctgtgtctta ttttatcttg gaactcaaag atccatcaca
     1021 gacaaaagta gtaacagaag aattacagaa aaataagaag tatactgtgc tttctcaaca
     1081 gagagttctt gatattgtga ttacctttat tcgtgttatt cagggattat tgattgtgct
     1141 ttcatcacaa gctattgtgg tagcagcggt tatgattggt atcattattt acatcaatat
     1201 catgcaacgt tccaaggaaa taggtgtcat gaaagcagtt ggttatcaga atcgtgatgt
     1261 caaaggaatt tttatttacg aggctatctg gattgtaggc atcgccttgc tgctggcatt
     1321 tttggttgca caaggggtgg gaagtttggc gaatgcgatt gtaagtcact tttacccatc
     1381 catcactaag gtttttgaat taaatctttt atctgtttta ggaactctag ttttcgctct
     1441 attacttggt tatgtctcag cctacttccc ggcgcgtaag attagtaaaa tggatcctgt
     1501 agaatcgtta cgctatgaat aataagggat tccttgattt acagtcttaa aaagaaactc
     1561 acatagaaaa acaatggcca tacttgcaat tcaactcaaa tagttgtata ataggtttgt
     1621 tgaaaggtga tttgtagtga ttcaagttac tcttttcaca ataaaaattt catgattttc
     1681 ataaggagga aatcactaat ggtagttaaa gttggtatta acggtttcgg acgtatcggt
     1741 cgtcttgctt tccgtcgtat ccaaaacgta gaaggtgttg aagttacacg catcaacgac
     1801 cttacagatc cagttatgct tgcacacttg ttgaaatacg acacaactca aggtcgtttc
     1861 gacggtactg ttgaagttaa agaaggtgga tttgaagtta acggtaaatt catcaaagtt
     1921 tctgctgaac gtgatccaga acaaatcgac tgggctactg acggtgtaga aatcgttctt
     1981 gaagctactg gtttctttgc taagaaagaa gcagctgaaa aacaccttaa aggtggagct
     2041 aaaaaagttg ttatcactgc tcctggtgga aacgacgtta aaacagttgt attcaacact
     2101 aaccacaacg ttcttgacgg tactgaaaca gttatctcag gtgcttcatg tactacaaac
     2161 tgcttggctc caatggctaa agctcttcaa gacaactttg gtgttgttga aggattgatg
     2221 actactatcc acgcttacac tggtgaccaa atgatccttg acggaccaca ccgtggtggt
     2281 gaccttcgcc gtgctcgcgc tggtgctgca aacatcgttc ctaactcaac tggtgctgca
     2341 aaagctatcg gtcttgtaat tccagaattg aatggtaaac ttgacggatc tgcacaacgc
     2401 gttccaactc caactggatc agttactgaa ttggtagcag ttcttgaaaa gaacgttact
     2461 gttgatgaag tgaacgcagc tatgaaagca gcttcaaacg aatcatacgg ttacacagaa
     2521 gatccaatcg tatcttcaga tatcgtaggt atgtcttacg gttcattgtt tgacgcaact
     2581 caaactaaag ttcttgacgt tgacggtaaa caattggtta aagttgtatc atggtacgac
     2641 aacgaaatgt catacactgc acaacttgtt cgtactcttg aatacttcgc aaaaattgct
     2701 aaataattct tgagttgata gaaagcaagg ctttgtggtc ttgctttttt atatggaaga
     2761 atggatgaca cgatcatcca ttctttttta attctttttc aaatgtattt gaaagggtag
     2821 tgaaagttag cttctctaaa gtaagtgggt gggtaaagga aagtcggaag gcatgaagca
     2881 taagccggct tgtctttgat ttactattat agagagggtc tcccaggata ggaagattat
     2941 gatgcgaaag gtgcacacga atctgatggg ttcgccctgt ctttagcttg caatgagcca
     3001 aggaagtctt gtttgagaat tgctttaatc tgcttacatg cgtttcagca tattgcccat
     3061 tttttgcatc aactattctt tttctacgat catggcgatc acgtccaatt ttgtctctga
     3121 aaacaagttc ttttctgttg atatttccat caactagagc ccaatattct ctagaaatct
     3181 cttttttctc caataagcga ttgagaatgg gcaggataaa aggatttttg gcaaagagaa
     3241 ctaagccact ggtttccatg tccagacgat gaacgacata gcaggtttgg ccaacatagg
     3301 tactgacatg gttaagaaga gcaatttcgt ttggttgatt accatgcgtt ttcatcccct
     3361 ctggtttgtt tacaataatc aagtgttgat cttgataaac ttcctgcact aagtctgggt
     3421 tgccccaagg gatcgtcttt tggggataat cttcctcgtc aaaagtcaac tggcaaacat
     3481 ctccaggatt tacgatttcg ttccagtgga cttcttcttg atttatcaaa atatgtttct
     3541 tgattctcaa aaaatgacgg atttttctag ggatgaggag ttgttcctca agtaattgct
     3601 ttaccgtcat ttgaggtaga gaggcgggta atgtaaatgt gaattgcata cagatattgt
     3661 aacaaaaaaa gccctatttg gataggaaat agctaaattc ttgtcttcct atgatgaaga
     3721 tgataaaata aacgcatgaa attagataaa ttatttgaga aatttctttc tctttttaaa
     3781 aaagaaacaa gtgaactaga ggactctgat tctactatct tacgtcgctc tcgtagtaat
     3841 cgaaaaaaat tagcccaagt aggtccgatt cgaaaattct ggcgtcgtta tcatctaaca
     3901 aagattatcc ttatactagg tttgagtgca ggcttgctag ttggaatcta tttgtttgct
     3961 gtagccaagt cgaccaatgt caatgatttg caaaatgcct tgaaaactcg gactcttatt
     4021 tttgaccgtg aagaaaaaga ggctggtgcc ttgtctggtc aaaagggaac ctatgttgag
     4081 ctgactgata tcagtaaaaa cttgcagaat gctgttattg cgacagaaga ccgttctttc
     4141 tataaaaatg acgggattaa ctatggccgt ttcttcttgg ctattgtcac tgctggccgt
     4201 tcaggtggtg gctctaccat tacccaacag ctggctaaaa acgcctattt atcacaggat
     4261 caaactgttg agagaaaagc gaaagaattt ttccttgcct tagaattaag caaaaaatat
     4321 agtaaggagc aaattctaac catgtacctt aacaacgctt attttggaaa tggtgtgtgg
     4381 ggtgtagaag atgcgagtaa gaaatacttt ggagtttctg catcagaagt gagtctggat
     4441 caagctgcga ctctggcagg gatgctcaag gggccggaac tgtataatcc cttgaattcc
     4501 gtagaagatt ctactaatcg gcgcgatact gtcttgcaga atatggttgc agcaggatat
     4561 attgataaaa accaagaaac cgaagctgct gaagttgata tgacttcgca attgcacgat
     4621 aagtatgaag gaaaaatctc agattaccgt tacccctctt attttgatgc ggtggttaat
     4681 gaagctgttt ccaagtataa tctaacagag gaagagattg tcaataatgg ctaccgcatt
     4741 tacacagagc tggaccaaaa ctaccaagca aatatgcaga ttgtttatga aaacacatcg
     4801 ctatttccga gggcagagga tggaacgttt gctcaatcag gaagtgtagc tctcgaaccg
     4861 aaaacagggg gagttcgtgg agttgtcggt caagttgctg acaatgataa aactggattc
     4921 cggaatttca actatgcaac ccaatcaaag cgtagtcctg gttctacaat taagccttta
     4981 gttgtttata cgccagcagt tgaagcaggc tgggctttga ataagcagtt ggataaccat
     5041 accatgcagt atgacaacta taaggttgat aactatgcag ggatcaaaac gagtcgagaa
     5101 gttcctatgt atcaagcctt ggcagaatcg cttaatctac ctgctgttgc cactgttaat
     5161 gatttgggtg tcgacaaggc ttttgaggca ggcgaaaaat tcggactcaa catggaaaag
     5221 gtcgaccgtg ttcttggtgt cgccttggga agcggtgttg aaaccaaccc tcttcaaatg
     5281 gctcaagcat acgctgcctt tgcaaatgaa ggtttaatgc ctgaagctca ttttattagt
     5341 agaattgaaa atgctagtgg acaagttatt gcgagtcata aaaattcaca aaaacgggtg
     5401 attgataagt ctgtagctga caagatgacc agtatgatgt tggggacttt caccaacggt
     5461 accggtatta gttcatcgcc tgcagactat attatggcag ggaaaactgg aacaactgaa
     5521 gcagttttca atccggagta cacaagtgac cagtgggtaa ttggttatac tccggatgta
     5581 gtgattagcc actggcttgg ctttccgacc actgatgaaa atcactatct agctggctct
     5641 acttcaaacg gtgcagctca tgtctttaga aacattgcca ataccatttt accctatacg
     5701 ccaggaagta cctttacggt tgaaaatgct tataagcaaa atggaattgc accagtcaat
     5761 acaaaaagac aagtacaaac caatgataat agccagacag atgataattt gtctgatatt
     5821 cgagggcgtg cgcaaagtct agtagatgag gctagccggg ctatctcaga tgcgaagatt
     5881 aaggaaaagg ctcaaacaat atgggattcg atagtcaatc tatttcgcta agatgcttgt
     5941 caaagcctag atttcttgtt ataatggata agatggaggc gttatggcac taaaaaaagc
     6001 aagcctagct tgtgcggttt gtggttcgag aaactattca atcaagatca gcggaaaccc
     6061 caagcctaca cgactagaag taaataaatt ttgtaagcat tgtggcaagt acactacaca
     6121 tagagaaacg agataggaga gagcgatgcg ttttattgga gatattttta gacttcttaa
     6181 agacacaacg tggccaactc gcaaggaaag ctggagagat tttcgttcta tcatggaata
     6241 cacagctttc tttgtagtaa ttatttacat ttttgaccag ttgattgttt caggtttgat
     6301 tcgatttatt aacatttttt agaagattag tggagttaat tacactagaa atcttctatt
     6361 tatgaaagga aatatcatgg atagttttga taaaggatgg tttgttttac aaacttattc
     6421 tggttatgaa aataaggtga aagaaaatct attacaacgt gcacaaacct acaatatgtt
     6481 ggataatatt ctacgcgttg aaattccaac acaaacagtg caagttgaaa aaaatggaaa
     6541 gagaaaagaa gtagaagaaa atcgctttcc aggttatgtt cttgtagaaa tggtcatgac
     6601 agatgaagct tggtttgttg ttcgaaacac accaaatgtt acaggatttg tcggatcaca
     6661 cgggaacaga tcaaaaccaa ctccattatt ggaacaagaa attcgtgaca ttttggtatc
     6721 tatgggacaa actgttcaag aatttgattt cgatgttgag attggtcaaa ctgtacgtat
     6781 tattgatggt gcttttgcag actacactgg taagattaca gaaattgata ataacaaagt
     6841 gaaaatgatt atctctatgt ttggtaatga cacagttgca gaagtaaacc taaaccaaat
     6901 cgcagaatta taa
//
LOCUS       DAVGJJ010000088         6870 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000088 DAVGJJ010000000
VERSION     DAVGJJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.088"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(230..493)
                     /locus_tag="VVE35_001741"
                     /pseudo
     CDS             complement(230..493)
                     /locus_tag="VVE35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            691..2457
                     /gene="patB"
                     /locus_tag="VVE35_001742"
     CDS             691..2457
                     /gene="patB"
                     /locus_tag="VVE35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU3061888.1"
                     /translation="MKTVQFFWNYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNVVLYIGLILV
                     MFSRNVTLALITIASTPLAFFMLIFIVKMARKSTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLVNTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2596..3285
                     /locus_tag="VVE35_001743"
     CDS             2596..3285
                     /locus_tag="VVE35_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU3061889.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3289..3468
                     /locus_tag="VVE35_001744"
     CDS             3289..3468
                     /locus_tag="VVE35_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061890.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3455..4804
                     /locus_tag="VVE35_001745"
     CDS             3455..4804
                     /locus_tag="VVE35_001745"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU3061891.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4928..6388
                     /locus_tag="VVE35_001746"
     CDS             4928..6388
                     /locus_tag="VVE35_001746"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU3061892.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6402..6836
                     /locus_tag="VVE35_001747"
     CDS             6402..6836
                     /locus_tag="VVE35_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU3061893.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2135 a         1319 c         1483 g         1933 t
ORIGIN      
        1 aaaagtccat tttttcaaag gtaatcctgt gttaatttca gaaattacat cactttttgt
       61 tcgtcaaatg gcagctcttt tttaggatat aaaacagggt tcggataagt ttttttgcaa
      121 ggtggatgat ggctacattg taatgttttc cttgttctaa tttagtctta agataggcct
      181 taaaagcagg cgaaaagcga gggcatgctt tggcagcttg tatgagtacc taccgcagat
      241 gaggggaacc ccgtttgacc attcttccag ctaaatcaat ctgacctgac tgataaatag
      301 aagaatccag tccagcgaaa gcttgtaatt gagcaggatt atcaaaggca tgaatatttc
      361 gaatctcggc taaaatgacc gcccctaaac gattcccaat cccagtaacc gtcgttatga
      421 tcgagttgaa ctcagccatc aagtcattga cacatgtttc cgccttgtca atgagcctct
      481 tgtaatgttt gatgttttca ttacacgaga taaaacgttt atgcgttatc aaactcatga
      541 ccaattgaaa caaaaagctg tggttagatc ctttcggaaa ttgtcaagcg attggaggaa
      601 atgaactaat ccacagcagc ttattccaag tataccactt gggctttggc agtagctaac
      661 tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt ttggaattat
      721 tttaaggtct acaagttctc atttgtagtt gtcatcttga tgattgttct ggcgactttt
      781 gcccaagccc tctttccagt cttctctgga caagcggtga cgcagctagc caatttagtt
      841 caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc
      901 aatcttggtc tgctggtttt ggttctattt atttctagtg taatatacat gtgtctcatg
      961 acgcgcgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg taagcttgct
     1021 cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcctgtc tcattttacc
     1081 agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat
     1141 gttgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc
     1201 atcaccattg ccagcacccc attggctttc tttatgctga ttttcatcgt gaaaatggca
     1261 cgcaaatcca ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag
     1321 agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga
     1381 tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga
     1441 attcttttcc ctgttatgaa tgggatgagc ctggttaata cagccatcgt catctttgct
     1501 ggttcggctg tgcttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt
     1561 gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt
     1621 tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca
     1681 gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa
     1741 atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga tgtcagcatt
     1801 tctgccccta aaggccagat gacagcagtt gttgggccga caggttcagg aaaaacgact
     1861 attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt
     1921 aaagacattc gtggctatga cttagatagt cttagaagca aggtgggaat tgtattgcaa
     1981 gattcggtct tgtttagcgg aacgattcga gacaatatcc gatttggtgt gccagatgct
     2041 agtcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt
     2101 ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc aacagggcag
     2161 aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat
     2221 gaagcaactt caaacgtaga tacggtgaca gaaagcaaga ttcagcatgc catggaggtg
     2281 gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca
     2341 gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt
     2401 ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa
     2461 agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta
     2521 aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc gttagcattc
     2581 gaaaggagag gcatcatggc tagaacggtt gtaggagttg ctgcaaatct atgtcccgta
     2641 gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt
     2701 caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt gcgtgattat
     2761 gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc tcagttttat
     2821 ggagagaaaa agaccgtcga gagcgatgat tacaatctgg tccgtgacga atttgaattg
     2881 gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc
     2941 caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa
     3001 ggactacctt tcgggacatc tcactctatt gagacagtag aaggaagcgt ggtggctaag
     3061 ctatttggga aagaaagtca ggtcaactca gtccatcgtc aaagcattaa agatttggca
     3121 cctaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct
     3181 atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa
     3241 gatggcaatt tagaattatt tgagtattta ttgaatgaac tgtaacgatt ggaacatctg
     3301 gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gcattttacg caaacgtttg
     3361 aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt
     3421 tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc
     3481 aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac
     3541 agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt
     3601 ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca
     3661 aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc
     3721 aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg
     3781 caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactt
     3841 ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac
     3901 aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca
     3961 agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt
     4021 tgaagcagac gctaacggtt ggggaacatt tgttgttcca gatgatatcg gtggacgctt
     4081 ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg acatcaaagc
     4141 tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa
     4201 cgaagcttac caatacgcag ccgttcgtaa catcctttat cgtaaaggct atgcaactga
     4261 gatcttggta aactatgagc catcacttca atacttctca gaatggtgga aacaattggc
     4321 tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac
     4381 tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt
     4441 tgtccgtgtt gacaaacctc gtaaaaacgt gcttattcct actttggaag aagaccttga
     4501 cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg
     4561 tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca
     4621 agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg
     4681 ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat
     4741 gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta atgcacgtct
     4801 ataatagaag aaaagagtgg tttgcccact ctttttactc tctttatcca tagaaattgg
     4861 actcagccaa gacttgtgat ataatataga aagcaaaaag gcagacgcct agataatagg
     4921 agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca
     4981 catcggaaat gctcgtacag cattgtttaa ctacttgtat gcgcgccatc atggtggaac
     5041 atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc
     5101 acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga
     5161 gaattatcgc cagtctgagc gtttggactt gtatcaaaaa tacattgacc aactattagc
     5221 tgaaggaaaa gcctataaat cttacgttac agaagaagag ttggcagctg aacgcgaacg
     5281 ccaagaagta gctggcgaaa caccacgcta catcaatgaa taccttggta tgagtgaaga
     5341 agaaaaagca gcttacatcg cagaacgtga agcagcaggg atcatcccaa ctgttcgttt
     5401 ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggcg atatcgaatt
     5461 tgaaggtggc aatatcggtg gtgactgggt tatccaaaag aaagacggtt acccaactta
     5521 caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta tccgtggaga
     5581 tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg gttgggaagc
     5641 tccagagttc ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa
     5701 acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga
     5761 agcagtcttt aactttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt
     5821 ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc
     5881 ctttgatcag aaaaaactag actggatgag caatgattat atcaagaatg cagaccttga
     5941 aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc
     6001 tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt
     6061 gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg aagtcatgac
     6121 gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga
     6181 tgataaattt gtgacagaaa atatcttccc acaaattaaa gcagttcaaa aagaaacagg
     6241 tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg
     6301 gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga
     6361 aaacatgcta aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga
     6421 tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca
     6481 atcatgttgt agctgcagta gaagcagaag atggacaaat atttacagga ttctgtatgg
     6541 agggaacctg tggtgttttc catctctgcg cagaacgggc ggccctcttc aatatgtacc
     6601 aattttcagg acaaactaag gttaagaaag tattagcctt tcgagacaaa ccaccttatg
     6661 gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagag cttaatgctg
     6721 aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt aaagtcgcag
     6781 aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag cgatagaaga
     6841 gtcagtataa ttttggctct ttttactata
//
LOCUS       DAVGJJ010000089         6825 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000089 DAVGJJ010000000
VERSION     DAVGJJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.089"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            87..3437
                     /locus_tag="VVE35_001748"
     CDS             87..3437
                     /locus_tag="VVE35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU3061894.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLEQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3708..3863
                     /locus_tag="VVE35_001749"
     CDS             3708..3863
                     /locus_tag="VVE35_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061895.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYDKATLTRIEEI
                     FEEYKSS"
     gene            complement(3978..4448)
                     /locus_tag="VVE35_001750"
     CDS             complement(3978..4448)
                     /locus_tag="VVE35_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU3061896.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4465..6738)
                     /locus_tag="VVE35_001751"
     CDS             complement(4465..6738)
                     /locus_tag="VVE35_001751"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU3061897.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIHEVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELKVKLPHKIELEKPQLNLVQPQGQAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2088 a         1148 c         1476 g         2113 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tggagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gaacaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagaca
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg agcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccacagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagtt ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagctgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagatgaaa
     3781 ctgacgaagt cagtaaccat acctacgaca aggcgacgtt gacgcggatt gaagagattt
     3841 tcgaagagta taaatcttcc taggataaag caaaacgcat agtatcaagt gttgaaaacc
     3901 cttgatacta tgcgttttct gatgttaaag actttctacc agttttttta agcataattg
     3961 ttagttgtag tcatttatta ttcttcaaag aaaaatggtg gggcgaattt tttcagttct
     4021 tcaaagcact tttgagcagt atctgcatct tcacagatga taagacagac atcattacca
     4081 caaagggtag cgatagcgtc agggaagctc aaagtatcaa tgatagaacc aaaggattga
     4141 gccagtccag gaagagtttt tagtaggact tggtgttgaa ctgggcgcat ccagacaagg
     4201 gcgtcttcca tgtagagttc gagacgtttt tcccattttg agatggaacc attgttaaga
     4261 acataataag cgctatcttc ttcgcggact tttgataggt tcatattttt gatgtcgcgt
     4321 gagagggttg cctgggttac ttgaatgtcg ttcttagcaa gaagggcttg caactcagcc
     4381 tgtgtatgaa tcttgttttt tgtgataaga gcgcgtataa gttggtggcg gtgttctgat
     4441 ttattcataa taatgtaact ccttttagca aggtaaggta agcatggact gagcgaggtc
     4501 gacagtcaag tggtagtctg tattgtcacg gatggtgatt tcaaagtcag tagtatagag
     4561 gactaaacgg agagtgtctc cttcttttag cttgtaaata gttggctgca gttcaaattg
     4621 aacgtccatc cattcatctg cagtaatatc ctctactaac agtaaatcat ttctattttg
     4681 taaattaagg taaccttttg tcacgactcg ttgtgcctct ggtctaaatg gcaattcaca
     4741 gagattttcc aacatgtgat agcgaccgtt gtcaatggtt ctagcactta aaatagctgg
     4801 ataaggttgt aggtatttct tttgcccaaa ttctagcagt tgggcagata agagcccctt
     4861 gtttgtactg gatttgatac gaagattgag ctgagcgcga ccgtttaggt ggagatcttt
     4921 agtcacagga aggttaatag taatctgatt agctttccct tgatagagct ctgtattgaa
     4981 ggtttggtat gtcttactat agcgctcaaa atccttatct gggtactggt tttgaatagc
     5041 ttgctcttct tgaccaagtg agaaggtttt acagttttct tgcccaccga agttatcaag
     5101 tgataaccaa gtctgtggag ctgtattgtc ctgccagata acagtaggaa gttgaaagtc
     5161 tgtttcctgt cctagtaatt tcttggttaa taaggcattt atggactcac ggaagtcaat
     5221 tgattgccaa ttgttcatgt aaacatgggc accattatgg aaaaagagat gcttgtgtat
     5281 atgagtagga agagcatgga acatctggta aacatgaagt ggtttgacat tccaatcctg
     5341 agaaccatga gtaaagacaa cctctgcctt tactttatgg gcattgagca gataattgcg
     5401 gtcatgccaa aactgattgt agtccccagt ttttcggtct agctgagctt tcactttttc
     5461 taagtcagct tggtgagctt cattgccacg gatatagtcg ccagctaaga gattacgaga
     5521 ataggttaac tcagcaaggg agtcaaagtc ctcacctgga taaccacctg ggctagtcac
     5581 cagaccgttt tcacggtagt agttgtacca tgatgaaatt cctgcctcgg caatgataac
     5641 ttctaaacca tcgactcctg tagtcgcaag accattggac atggtaccta gataggaaag
     5701 tcccgttgta gcaacttttc cgtttgacca atcagccttg acttgacgct ggcgcgtgtg
     5761 atcagtaaag gcacggcaac ggccgttaag ccaatcgatg acatttttat aagcctcgat
     5821 ttgctggtag tctccattag tcatgaaacc tgtcgagtct ttggtaccaa cacctgagac
     5881 atagagattg gcaaagcctc tcggaaggaa gtagtcgttt agtgtatagc tagagttgat
     5941 gtgagttagc ttttcctcag cctctgctat aagctcagct tgaccttggg gttggacgag
     6001 atttagttga ggtttctcta gctcaatctt gtgaggaagc ttaaccttaa gctcgccctc
     6061 catcttgtag agagccttgt cactagcctt gtcattggtt ccctgatgat aagggctggc
     6121 tgtcatgatg gcagggattt ttccatcaaa acgagggcga ataatgctaa cctttactag
     6181 gtctgatagc cctttttggt cagtatcgac acgagactca acgtaaacga cttcatgaat
     6241 gacatcctgg ttagaaaaag tagccaaact cttgccgtta aagtagtggt agtcattatc
     6301 ctccggaata agaccatcac taacaagttg gtcgataaga gtatttcctt ttttggtgcg
     6361 agtattgagt aactgataga gattttcaat caagtcacca tatataatgg gaaatccagt
     6421 ttctttacga aaaacgtcac tatcttcgaa gtcaaccaaa taagaaaagc ctaaaagttg
     6481 aaaagcaaca gtataaaaaa tatctgctgt cagttcatct tctgattgaa aaaatgtcag
     6541 caggtctgtt tttttatcag ctgctaggat agaaagtggg tagttggtgt cttgataagt
     6601 gaaaaagaaa cgacgtaaaa aggtttcaag tgagtctttg tgattggctg tattttgtaa
     6661 atcaaagcca cattttttta gttcagataa gacattttct tttggaaaat tgatataact
     6721 atattgatta aaacgcatag aacctccata tagaatgaca gttaaggtta ttatatcaaa
     6781 aaaaaagcag aaagggaatt gttaacttca aaaggaaata atcca
//
LOCUS       DAVGJJ010000090         6812 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000090 DAVGJJ010000000
VERSION     DAVGJJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.090"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..268)
                     /locus_tag="VVE35_001752"
     CDS             complement(<1..268)
                     /locus_tag="VVE35_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061898.1"
                     /translation="MNRLKELRKRDKITQVAFAKDNGIPLRTLQSWENGENQIKPEKA
                     QQLADIFGVSVGYLLGFNIDDVTEDEINFHNNVMERMNKEAFIRF"
     gene            469..666
                     /locus_tag="VVE35_001753"
     CDS             469..666
                     /locus_tag="VVE35_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061899.1"
                     /translation="MKNNMRLLLAKQRKKTADVAEATGISKSTLTALYYERAKNPSLD
                     TLKKVSSYLGVTLDEFLDTKE"
     gene            897..1499
                     /locus_tag="VVE35_001754"
     CDS             897..1499
                     /locus_tag="VVE35_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU3061900.1"
                     /translation="MELVYMDGKKEPYTLSSIVADCAEIKHRHLKILLNKHREDFESF
                     GKVQFKISPSKSGQNVRDYILNEQQATLMITYLRNTEPVKEFKKNLVKAFFEMRDELS
                     KRYLQRELEKPKRKTLTEAIKSWEKAPKHAYSTLTNLLLKGATGKNKAQLMQERESEN
                     GIDSLTSAELTNYQRLEDMAIAMINLNREYSEIKELIFKA"
     gene            1511..1771
                     /locus_tag="VVE35_001755"
     CDS             1511..1771
                     /locus_tag="VVE35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061901.1"
                     /translation="MENEFKTVTNAKGLEIPKYPKDFKKLVEKDRQLAEYLCMNYENL
                     DNEDLGAFLETVEQGFSWILDLIESKDLLYKPKSGSNHAKRK"
     gene            2108..2308
                     /locus_tag="VVE35_001756"
     CDS             2108..2308
                     /locus_tag="VVE35_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061902.1"
                     /translation="MTLDLDNMTRSEFDKLTTKIKDRNPNLFQFIIDFLDDKVSTEEV
                     YDFLKMERSYQVNYIKNYKARA"
     gene            2310..2525
                     /locus_tag="VVE35_001757"
     CDS             2310..2525
                     /locus_tag="VVE35_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061903.1"
                     /translation="MNELDLSNTQALIFTVILIGFLIYLNHRDRKKSAQLERENRKLG
                     ERPSESLSPDYGRYIQLAGINKQGDIK"
     gene            2522..2773
                     /locus_tag="VVE35_001758"
     CDS             2522..2773
                     /locus_tag="VVE35_001758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061904.1"
                     /translation="MNYRIYLHTKEQLEEHGLTREAFIVKRINNIKNLSLILGALNNH
                     LSDEDLRYISKTIETILNDQEDILYGTMSNHITFAYKPA"
     gene            2820..3101
                     /locus_tag="VVE35_001759"
     CDS             2820..3101
                     /locus_tag="VVE35_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU3061905.1"
                     /translation="MFSLSKESEQDLTNRISTVVENYLAVRERPKPRLTGLMSAQEAM
                     DELDIKYKTLQKWESAGLRRYQPPLEDTRKVYYKVTDILKFLGVDDGKD"
     gene            3088..3948
                     /locus_tag="VVE35_001760"
     CDS             3088..3948
                     /locus_tag="VVE35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEU3061906.1"
                     /translation="MAKTKIYFWLKVDKKFFDNLFIKRLKNMPGGYTMTVIYIRLMLE
                     SLEDDCILYYEGYFDSLVQELALKLDVSEDDINMTVAYFTRCGLIQIDDDGHATLSQA
                     KAMVESETNWSKYKREQRKQAPNVARLENVQNSWTNSNSCPTEIEKENRVNSKSNNLY
                     LDNILSGNPDFTFPTWLEETGIKDLEKTKYKELWIPIVYLNQVANKRYKFVDKTKRLL
                     LARFKEGYTLEDFKQVIDIKTAEWKDSPEFSKYLRPETLFGSKFDGYLNQKPKTIKGK
                     SEDNFPDLPF"
     gene            3960..4804
                     /locus_tag="VVE35_001761"
                     /pseudo
     CDS             3960..4804
                     /locus_tag="VVE35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4810..4965
                     /locus_tag="VVE35_001762"
     CDS             4810..4965
                     /locus_tag="VVE35_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061907.1"
                     /translation="MDEMKFSTEKGFIVYEKCGIIEIEKIPRFGEITLVYSDGKFTHL
                     VKKETKK"
     gene            5055..5276
                     /locus_tag="VVE35_001763"
     CDS             5055..5276
                     /locus_tag="VVE35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061908.1"
                     /translation="MSGDTSLGYVVANKFSMDPDKRQKIFSQCKKEGDSLEQRKQEIL
                     EKYANKQDKPKSRKNDSKGSESHKRKAKN"
     gene            5334..5684
                     /locus_tag="VVE35_001764"
     CDS             5334..5684
                     /locus_tag="VVE35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU3061909.1"
                     /translation="MSLTSDLANEIAKTLEAYSEEVEEQIDLIAEEVTNEAVNELKQT
                     SPKRYGKYAKNWRFKKNSKGSFVIYNADPTYRLTHLLEHGHVLRNGGRSKAIPHIKPV
                     EEKIKEKFEQKIKK"
     gene            5721..6572
                     /locus_tag="VVE35_001765"
     CDS             5721..6572
                     /locus_tag="VVE35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU3061910.1"
                     /translation="MAKKFSLVEKYVRSRGMSSDDGKSKTDLILSQDITSIYDVPEEG
                     KELVDLVNVIEHTGTGGTYETVGFYDEHLSELESEDFRDSKSVELRKKQIKTKFEHKT
                     FSGRIPLSSEQVDDGEYNISDFLSNKITRLCRKTRNIEIGKILKEAPEKNVSNFDELK
                     DTINDLNPERHNTIVLSQSLFKFLDKEKSSDGNYILKINKKERFSENLYVDDVIVVSD
                     EVLGVKGDKVAFVGDLYNFATLFERNKNSLRWVSESVIYGMSLMLYTRFVVKKIETDC
                     AFFIKWN"
     gene            6583..>6812
                     /locus_tag="VVE35_001766"
     CDS             6583..>6812
                     /locus_tag="VVE35_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061911.1"
                     /translation="MDIREVLSILENLDDKKDKIAKARTKLEEKRKTITGEKKVSFDN
                     IDSFFENNATSLEQITKMSESIDLLEKEYDTY"
BASE COUNT         2609 a          963 c         1376 g         1864 t
ORIGIN      
        1 aaaatctaat aaatgcttct ttgttcatcc tctccataac attgttatgg aaattgattt
       61 catcttccgt aacatcatca atattgaatc ctaacagata tccaacactg actccgaata
      121 tatccgctag ttgctgggct ttttctggtt ttatctggtt ttctccgttt tcccaacttt
      181 gtaatgttct tagaggtata ccattatctt tagcaaaagc tacttgcgtg attttatctc
      241 gttttctcaa ttcctttaat ctattcattt atatcaacac ctttcaaaga ttattatatc
      301 acgttttcaa aatccaaacg caaaaaaatc gcatcttttt aaaaaaaatg attgacagcg
      361 caaaaaataa gcggtataat tcaaaatgta acgcatatat cttgcgttat aattcaacac
      421 tttttaaatt attcaactga tattttccaa taaaggaggt ttagtactat gaaaaataac
      481 atgagattat tattagccaa gcaacgtaag aaaactgctg atgttgcaga ggctacagga
      541 atttcaaaaa gcactttaac ggctttgtac tatgaacgtg caaagaatcc aagtcttgat
      601 acgttaaaaa aagtatctag ttacttgggc gttacgctgg atgaatttct agatacgaaa
      661 gaatagaaag gagcgaacca atcgtaatac tactctacat ttatagattt ctcatgtggt
      721 gctttactac tggggattga taaacggatc tagctaaata ttcgcttgct tgctacctat
      781 agcagtatca agggtttgta ggggttcata ttctccgatt ttaccctact ttaatgcttt
      841 accttggtac tgttttaggt ggcaagcacg agcaacaaga agaaaggagc gaaccaatgg
      901 aattggttta tatggacggc aagaaagagc cgtatacact gagcagtatc gttgccgact
      961 gcgctgaaat taagcacaga catttgaaga ttttgctgaa caagcaccga gaggactttg
     1021 aaagcttcgg aaaggtgcaa tttaaaattt caccttcaaa gagtgggcaa aatgtacggg
     1081 actatatttt gaatgagcaa caagcaacct tgatgatcac ttacttacga aatacagaac
     1141 ccgtaaaaga gtttaagaag aacctggtta aagccttttt tgaaatgcgt gatgaacttt
     1201 ctaaacgcta tcttcaaaga gaactggaaa agccaaagcg caagacctta accgaagcta
     1261 tcaaatcatg ggagaaagcg cccaagcatg cctatagtac acttacaaac ctactgctaa
     1321 agggagcgac tgggaaaaac aaagcccaac tcatgcaaga gcgagaaagt gaaaacggta
     1381 ttgacagttt aacaagcgcg gaactgacaa actaccaacg tttggaagat atggcaatag
     1441 ctatgattaa cttgaatagg gaatattcag aaattaagga attaattttt aaagcatagg
     1501 aatatagaaa atggaaaatg aatttaagac agttacaaat gccaagggtt tagaaattcc
     1561 caagtatccc aaggatttta aaaagctagt tgagaaagac agacaactag ccgaatatct
     1621 ttgtatgaac tacgagaact tggacaatga agacctaggc gcatttcttg aaacagtgga
     1681 gcagggattc agctggattc tggatcttat cgaaagtaaa gatttgcttt ataaaccaaa
     1741 gtcaggtagt aatcatgcaa aaagaaaata aaaaaatcac ttgctcaaat tttgaccgag
     1801 gagagcaagc gacaagatta aggatataga aattttttct atgctccgat tatatcagaa
     1861 aacagctatt ttatcaaata cataaagaaa aaccgaagag caggcaagca attagaaaag
     1921 gttttgaaat caagtgctga cagggtgatt ctaaggcctt gtttagctga aagatgggta
     1981 attactcacg aaacaccgct acaagcgttc gccaacttgg ggcaactgcc cagcgtttgg
     2041 agtggtggag cataccatat agaaaacagg caagaaaaag gacaaggaaa ggctaatgga
     2101 gaaaaatatg actctagacc tagataacat gacacgatca gaatttgata agctaacgac
     2161 taaaatcaag gatagaaatc cgaacctctt tcagttcatc attgactttt tagatgataa
     2221 agtaagcacc gaagaggtgt atgattttct gaagatggag cgaagctatc aagtaaatta
     2281 tatcaagaat tacaaagcga gggcatagca tgaatgaact agatttaagc aatacacagg
     2341 cgcttatttt taccgtgatt ttgattggct ttctaattta cctaaaccat cgagaccgca
     2401 aaaagagcgc ccaactggag cgagaaaaca ggaaactagg agaaagacct agtgagagtt
     2461 taagccctga ctatgggcgg tatatccaac ttgcaggtat taataaacaa ggagatatca
     2521 aatgaattat agaatatatt tacatactaa agagcaacta gaggagcacg gattaacaag
     2581 agaggctttt atagttaagc gtataaacaa cataaaaaat ctatcactaa ttttaggagc
     2641 attaaataat catctttcgg atgaagactt aagatatatt tcgaaaacta ttgagacgat
     2701 tttaaatgat caggaagata ttttatatgg cacaatgagt aaccatataa ccttcgccta
     2761 taaaccagcc tagaaaacga ggaatagaat gaattttata ctgaaagggg tggaaggata
     2821 tgtttagttt gagtaaagaa agcgaacaag atttaacaaa tagaataagc acagtagtag
     2881 aaaactatct agcagtccga gaaagaccaa aaccacgact aactggttta atgtcagcac
     2941 aggaagccat ggacgagtta gatataaaat acaaaacctt gcagaagtgg gaaagcgcag
     3001 gactaagacg gtatcaacca ccactagaag atactagaaa ggtttactac aaagttacgg
     3061 acattttgaa gttcctgggg gtagatgatg gcaaagacta aaatatattt ttggttaaaa
     3121 gttgataaga agttttttga taatcttttt attaagcgac ttaaaaatat gcctggtggc
     3181 tacactatga cagtgattta tatccgtctt atgttggaaa gtttagaaga tgattgtatt
     3241 ttgtactatg aaggatattt tgatagtttg gtacaggaat tagctttaaa actggatgtt
     3301 tctgaagatg atataaatat gacggttgca tattttacaa gatgtggact gattcagatt
     3361 gatgatgatg gccatgctac attatcgcaa gcaaaagcca tggttgagag tgagacaaac
     3421 tggtctaaat ataaacgtga acagcgaaaa caagccccaa atgtggcaag attggaaaat
     3481 gtccaaaata gttggacaaa ttccaactca tgtccaacag agatagagaa agagaataga
     3541 gttaatagta agagtaataa tttatattta gataatatat tgtcgggaaa tcccgacttc
     3601 acttttccta cttggcttga agaaacaggt ataaaagatt tagagaaaac aaaatataaa
     3661 gaactttgga ttcctattgt ttatctgaat caagtagcta ataagaggta taagtttgtt
     3721 gataagacaa aaaggctttt actagcacga ttcaaagaag gctatacact tgaagatttt
     3781 aaacaggtga tagatattaa aacggcagaa tggaaggata gtcctgaatt ttctaaatat
     3841 ctgagacctg aaacactttt cggatctaag tttgacggtt atttgaatca aaagcctaaa
     3901 accataaaag ggaagtctga agataacttc ccagatctac cattttagga gttgcaaaga
     3961 tgaaggaaca atttaaagag ttcaataaca aaaaaatatc ggataaagtt tgcgatattc
     4021 accaggtaaa ttactgggaa atttctatac ctgtagtagg gagttcagaa agaaaaatac
     4081 aaccattttg cccggaatgt gtgaagggag acattaaaca aaaagagcaa gacctattac
     4141 agaggtttga tgatagacaa acatatttca aaacttatga tgtcttaatg cgtgatagta
     4201 cgattcctaa cgagttgaag gggcaacgtt tgataatttc tttgtgaaga cgacagagga
     4261 aggtcagatg ttagagtttg taaaggggca agctcagaaa taccttgcag gtatgacggg
     4321 aaatacttta atcagcggta gcacaggaat aggaaaaagt catttatcgc ttgccctggc
     4381 caaagaaatc aatgagagtt tcagagagaa gaacgatcct aagagtgtct tatttgtcag
     4441 cttaaccgag attatcaagc agataaaaga aggttgggct tatggaagaa atgcaaactt
     4501 aacagagtat gaggcagtta aaaagctagt tgatgtagat tttctaatca tcgatgacct
     4561 aggggcaaaa aatgggacgg taactcctaa gagtgactgg gaacaggatt tcttgtttga
     4621 tattatcaac aatcgagaaa ctacgatttt caacacgaac ctagatagta gcgaactgcg
     4681 gatggtatac aatgctagaa actcaagtag aattttgaaa ggtttagaag ggaacacttt
     4741 caaggctttt acaatcaaag ataagcgata tacgattaac acagtgagag gagagaaagg
     4801 ttaatagata tggatgaaat gaaattttca acagaaaaag gctttattgt ctacgaaaaa
     4861 tgtggtataa tagagataga aaaaattcca agatttggag aaataacttt ggtctactca
     4921 gatgggaaat ttactcatct agtcaaaaaa gaaactaaaa aataagtcta ttgagaacaa
     4981 ctcagggaca taccgtaagc atttagtgct agtggtatgt ccctttttgt ttgaatagaa
     5041 agggggtgag tattatgtca ggagatactt ctttagggta tgtagtagcc aataagtttt
     5101 ctatggatcc agataaaaga cagaaaatct tttctcagtg taaaaaagaa ggtgatagct
     5161 tagaacaacg gaaacaagaa atactagaga aatatgctaa caaacaagac aaaccaaaat
     5221 ctagaaaaaa tgattctaaa ggctcggaga gtcataaaag aaaagctaag aactaagaac
     5281 tttagaaaaa attataaaca acgaggagca ataaagagat aaaggaggat aaaatgagtt
     5341 taacgagtga tttagcaaat gaaattgcta aaactttgga agcttattct gaagaagttg
     5401 aagaacagat agatttaatt gctgaagagg ttacaaatga agcagtgaat gaattgaaac
     5461 aaacaagtcc taaaagatat gggaaatatg cgaaaaattg gcgctttaag aaaaattcta
     5521 aaggttcttt tgtaatctat aatgcagatc caacatatag attaactcat ttattagaac
     5581 atggacatgt attaagaaat gggggacgca gcaaagcaat tcctcacata aaaccagtag
     5641 aggaaaaaat aaaagagaaa tttgaacaaa aaataaaaaa ataggcagat aatctaaaaa
     5701 ataagataaa ggagtataaa atggctaaaa aatttagttt ggtagaaaag tatgtaagaa
     5761 gtagaggaat gagtagtgat gatggaaaat caaaaacaga tttaatatta tcacaagata
     5821 taacaagtat ctatgacgtt cctgaagaag ggaaagaatt agtggatctt gttaatgtga
     5881 ttgagcatac gggtactggt ggaacatatg aaactgtagg tttttacgat gaacatctat
     5941 cagaacttga atcagaagat tttagagata gtaaaagtgt agaacttaga aaaaaacaga
     6001 ttaaaaccaa gtttgaacat aagacatttt caggacgtat tcccttatcg tctgaacaag
     6061 ttgatgatgg agaatataat atatcagact tcttaagtaa caaaattacc cgtctttgtc
     6121 gtaaaactcg taatattgaa attggaaaaa ttctaaaaga agcacctgaa aaaaatgttt
     6181 ctaattttga cgaattgaaa gatacaataa acgatttaaa tcctgaacgg cataatacta
     6241 ttgtattaag tcagtcacta tttaagtttt tagataaaga gaaatctagt gatggaaatt
     6301 atattttaaa aattaacaag aaggaacgat tctcagaaaa cttatacgtt gatgatgtta
     6361 ttgttgtatc tgatgaagta ctaggagtaa aaggcgataa agttgctttc gttggggatt
     6421 tgtacaattt cgccacttta tttgaaagaa ataaaaatag cttacgttgg gtaagtgaat
     6481 ccgttattta tggaatgagt ttaatgcttt atactcgttt cgttgtgaag aaaattgaaa
     6541 cggattgcgc tttctttata aaatggaatt aggagatagg ggatggatat tagagaagta
     6601 ttatcaatat tagaaaatct tgatgataaa aaagataaga ttgcaaaagc aagaacaaag
     6661 ttggaagaaa aaagaaaaac aattactgga gagaagaagg tttcgtttga taatattgat
     6721 tctttttttg agaataatgc tacttcctta gaacaaatta ctaaaatgag tgaatcaatc
     6781 gatcttttag agaaagagta tgatacttat tt
//
LOCUS       DAVGJJ010000091         6508 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000091 DAVGJJ010000000
VERSION     DAVGJJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.091"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            8..1426
                     /locus_tag="VVE35_001767"
     CDS             8..1426
                     /locus_tag="VVE35_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU3061912.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALIVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            1480..2187
                     /locus_tag="VVE35_001768"
                     /pseudo
     CDS             1480..2187
                     /locus_tag="VVE35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            2282..3256
                     /gene="folP"
                     /locus_tag="VVE35_001769"
     CDS             2282..3256
                     /gene="folP"
                     /locus_tag="VVE35_001769"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU3061913.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            3258..4580
                     /locus_tag="VVE35_001770"
     CDS             3258..4580
                     /locus_tag="VVE35_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU3061914.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            4561..5115
                     /gene="folE"
                     /locus_tag="VVE35_001771"
     CDS             4561..5115
                     /gene="folE"
                     /locus_tag="VVE35_001771"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU3061915.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            5158..5970
                     /gene="folK"
                     /locus_tag="VVE35_001772"
     CDS             5158..5970
                     /gene="folK"
                     /locus_tag="VVE35_001772"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU3061916.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLET
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(6015..6332)
                     /locus_tag="VVE35_001773"
     CDS             complement(6015..6332)
                     /locus_tag="VVE35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU3061917.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT         1798 a         1229 c         1552 g         1929 t
ORIGIN      
        1 attgatgatg gacaaattat ttaaactaaa agagaacggt acagacgttc gtacagaggt
       61 tctcgctggt ttaacaactt tctttgcaat gagctatatt ctctttgtaa acccacaaat
      121 actttcacaa acaggaatgc ctgctcaggg cgtcttccta gcgacgatta ttggtgcagt
      181 agcgggtacc ttgatgatgg ctttttatgc taacttacct tatgcccaag cgccaggtat
      241 gggactcaat gccttcttta cctttacagt tgtattcggg cttggttatt cttggcaaga
      301 agccctagct atggtcttca tctgtgggat tatttcattg attattacct tgacaaatgt
      361 tcgtaaaatg atcattgaat cgattcccaa tgctcttcgc tcagctattt cagctggtat
      421 cggtgtcttc cttgcctatg tagggattaa gaatgctgga cttttgaaat tcacgattga
      481 tccaggcaac tatactgttg taggagaagg ggctgacaaa gctcaagcaa cgattgcagc
      541 aaactcttca gcagttccag gattggtcag ctttaataat ccagctgttt tagtggctct
      601 tgcaggactt gccattacta tcttctttgt catcaaaggg attaaagggg gaattattct
      661 ctctatcttg acaacaactg ttcttgctat tgcagttggt ttggttgatt tgtctagtat
      721 cgattttgct aataaccatg ttggtgcagc ttttgaagat ttgaagacaa tctttggtgc
      781 agctcttggt tcagaaggtt taggagcttt ggtttcagat acagctcgct tgcctgaaac
      841 tctgatggcc attcttgcct tctcattgac agatattttt gacacaattg gtaccttgat
      901 cggtacaggt gaaaaagttg gtatcgtagc gacaaatggt gaaaatcacc aatcagccaa
      961 attggacaag gctctttact ctgatttgat tggaacgaca gtcggtgcca ttgcaggtac
     1021 ttcaaacgta acgacttatg ttgagtctgc tgctggtatc ggtgcaggtg gacgtactgg
     1081 tttgacagcc ttgattgtag ctatctgttt tgcgatttca agcttcttta gcccacttct
     1141 agcgatcgta ccaacagcgg ctacagctcc aatcttgatt atcgttggga ttatgatgct
     1201 tggtagcttg aaaaatatcc attgggatga tatgtctgaa gcagttcctg ccttcttcac
     1261 atctatcttt atgggattca gctactctat cactcaaggg attgcagttg gtttcttgac
     1321 ttacactttg actaagcttg ttaaaggtca agttaaagat gttcatgtca tgatttggat
     1381 tttggatgcc ttgtttatcc ttaactacat cagcatggcc ttataataga atgacccagg
     1441 gggatttccc ccctttttta atacaaaagg agataggtga tgaaagagaa aaatatgtgg
     1501 aaagaattgt tgaatcgtgc aggctggatt ttggtctttt tacttgccgt ccttttatat
     1561 caggttcccc tagtggttac ctctattttg actttaaaag aagtagccct gctacagtca
     1621 gggctgatag ttgctggcct ttcaattgtg gttctggctc tatttattat gggagctcgt
     1681 aaaaccaagt tagctagttt taatttttct ttttttagag ctaaagattt ggcacgtttg
     1741 ggcttgagtt atctagttat tgtcgggtca aatatacttg gttccatttt attgcaactg
     1801 tcaaatgaga cgacaacagc taaccagtct cagattaacg atatggttca gaatagttct
     1861 ctgatttcta gcttcttctt gctagccttg cttgctccaa tttgtgagga aatcttgtgc
     1921 cgtgggattg ttcctaaaaa gattttccga ggcaaggaga acttgggatt tgtagtcggt
     1981 acgattgtgt ttgctttatt gcatcaacca agtaatttac cttctttgtt gatttatgga
     2041 ggtatgtcga cagttctatc ttggacggcc tgcaagaccc aacgtttgga aatgtcgatc
     2101 tagcttcaca tgattgttaa tgggattgct ttctgtttgt tggctcttgt ggtgattatg
     2161 agtcggacat taggaatttc tgtgtaaaag tttttatgta ggaaccgacc tctttctact
     2221 agggaaagat gaatgcaatc gtgtccatct ttttcttttt atggtaaaat agaaaaataa
     2281 tatgatgaaa atccttgagg gagtgaccga tatgtcaagt aaagccaatc atgcaaagac
     2341 agttatttgc ggaattatca atgtaacccc agactccttt tcggacggtg gtcaattttt
     2401 tgctcttgag caggcgctcc agcaggctcg taaattgata gcagaaggag ccagtatgct
     2461 cgatatcggc ggagaatcga ctcggccggg tagtagctat gttgagatag aagaggaaat
     2521 ccagcgtgtt gttccagtga tcaaagcgat tcgcaaggaa agtgatgtcc tcatctctat
     2581 tgatacttgg aagagtcaag tagcagaggc tgctttggct gctggtgccg atctagtcaa
     2641 tgatatcact ggtcttatgg gtgatgagaa aatggctcat gtggtagctg aagcgagagc
     2701 gaaagtggtc atcatgttta acccagttat ggctcgacct cagcatccta gttcgcttat
     2761 cttccctcat tttggttttg gtcaaacctt tacagaaaaa gagttagctg actttgaaac
     2821 attgccaatc gaagacttga tggtggcttt ctttgaacga gcactagcga gagcggcaga
     2881 agctggtatt acaccagaaa atatcctgtt ggatccagga attggctttg gtctgaccaa
     2941 gaaagaaaat ctcctccttt tacgggacct ggataaacta catcagaagg gctatccaat
     3001 ctttctcgga gtgtcgcgca agcgatttgt catcaatatc cttgaagaaa gtgggtttga
     3061 agtcaatcct gagacagagc ttggtttccg aaatcgggac acggcttcgg ctcatgtaac
     3121 tagtatcgct gcgagacagg gtgtagaagt ggtgcgcgtg catgacgtag ctagtcacag
     3181 gatggcagtt gaaattgcct ctgccattcg tctggctgat gaagcggaaa atttagattt
     3241 aaaacaatat aaataagatg aaagaaattg aaaacaatca gtggattgct aactaccgga
     3301 cggatcaacc gcattttgga ttggaacgaa tggtggaact gttagctttg cgtggcaatc
     3361 cccatctcaa actcaaggtc ctccatatcg gagggactaa cggcaagggc tcgactattg
     3421 cttttttgaa aaagatgcta gaaaagctag ggctgagagt tggcgtgttt agctcgccct
     3481 atctcattca ttacacagac cagattagca tcaatgggga atcgatctca gaagcgaggc
     3541 tagaagctct catggcagac tatcagtctt tgctggaggg agaagcggtc gccaatttac
     3601 agggcacaac cgagtttgag attatcacag ccctggccta tgactacttt gcctcagagc
     3661 aagtagatgt ggccatcatg gaagttggca tgggtggact tttggatagt accaatgtct
     3721 gtcagcccat tttgacagga attacaacta ttggcttgga tcatgtggcc ctacttggtg
     3781 acaccttgga ggccatagca gagcagaagg caggtattat caaacaaggg atgcccttgg
     3841 taacagggcg tattgctcca gaagccttga ctgtgattga ccgcattgcg gaagggaaag
     3901 atgcgccgag acttgcctac gggacagatt atcaggttcg tcatcaagaa agtgtggtga
     3961 caggggaagt ctttgactat acaagtgctg tcagacaagg tcgcttccag actagcctgc
     4021 ttggtttgta ccaaatagag aatgctggga tggccatagc tttacttgat actttttgtc
     4081 aagaagatgg tcgagagcta gcaagcaatg actttcttgg tcaagccttg gaagaaacaa
     4141 gttggccagg gcgtttggaa atcgtgtcaa gagatccctt gatgattttg gatggagccc
     4201 ataatcccca tgctatcaag gccttgttgg taaccttgca agaacgtttt gcggattatc
     4261 ataaggaaat cctcttcact tgtatcaaaa ccaaggcctt ggaggatatg ttggatttgc
     4321 tgggagccat gccagatacc gagcttactc taacacattt tgcggatagt cgggcgacgg
     4381 atgaaagcgt gctgaaagag gcagctaagt ctagaaatct cagctaccaa gattggcatg
     4441 attttctaga gcagaatttg acagataaaa aagaagagaa acaaacagtt aggattgtca
     4501 caggttcctt gtatttcttg agccaagtga gggcctatct gatggagagg aagaacgaga
     4561 atggatacac aaaagattga agcagctgta aaaatgatta tcgaggctgt aggagagaac
     4621 gctaatcgcg agggcttgca ggaaacacct gctcgtgtag cccgtatgta tcaagagatt
     4681 ttttcaggtc ttggtcaaac agcagaggaa catttgtcaa aatcctttga aattattgac
     4741 gataatatgg tggtagaaaa ggatatcttt ttccatacca tgtgtgaaca ccacttcttg
     4801 ccattttatg gtagagcgca cattgcctac attccagatg gtcgtgtggc aggcttgtct
     4861 aagctagccc gtacggttga agtttattcg aaaaaaccac aaattcaaga acgtttgaat
     4921 atcgaagtgg ccgatgcctt gatggactat ctaggtgcta aaggagcctt tgttgtcatt
     4981 gaggcggaac atatgtgtat gagtatgcgt ggtgttagaa aaccaggcac tgcaaccttg
     5041 acgacagtag ctcgtggtct atttgaaaca gataaggatc tccgtgacca agcttatcgt
     5101 ttaatggggc tataaaaaga atccgcttca agcggatttt tctagaaagg aatcattatg
     5161 gatcaactgc agattaagga tttggaaatg tttgcctatc atggtctttt tcctagtgag
     5221 aaagaattgg ggcagaaatt tgtcgtttca gccatcctat cctatgatat gaccaaggca
     5281 gctacagact tggatttaac agcctctgtc cattacggag aattgtgtca gcagtggacg
     5341 acttggtttc aggaaacgag tgaagatttg attgaaacgg tagcctataa actggtggaa
     5401 cgtacctttg agtcttatcc tcttgtccaa gaaatgaagt tggaactgaa aaaaccttgg
     5461 gcaccggtgc atttgtcact agatacttgc tcggtaacca ttcatcgccg caagcaacga
     5521 gcctttatcg ccctaggaag caatatggga gataaacaag taaacttgaa gcaagccatt
     5581 gacaaactgc gagctcgtgg catccatatt ctcaaagagt ccagtgtctt agagacggag
     5641 ccttggggtg gagtggagca ggatagcttt gccaatcaag tggttgaggt ggaaacctgg
     5701 ctaccagcac aagacttgtt agaaaccttg ttagccattg agtcagagct gggacgggtg
     5761 agagaagtac attggggacc tcgtttgatt gatttggact tgctctttgt ggaggaccag
     5821 atcctttata cagacgacct catattacct catccttaca tagcggaacg cctttttgtc
     5881 cttgagtcct tacaggaaat tgcgcctcat tttatccatc cgatattaaa acaaccgatc
     5941 cgcaacttgt atgatgcttt gaaaaaatag aaaaactcta gttttcagtt acttgcaact
     6001 gaaggctaga gtttttaaaa taggtcattt tcttctggga ggaggatagt ttctctaccg
     6061 tccatgtcta aaaccagtac tcttggggga taacgagggt cgaaagggtg gttaaagtca
     6121 aaatcaatgg ctgtagggag gtgttgactt gaaaagtgga aggtaatctt tccttggtta
     6181 ttaagcaatt gaaactcgag ttcttcttcc aattcaaaga cattttttaa gaaatgatcg
     6241 atgatatacc aaaaagagtc aatgatgtca tcgggcaagc tggtaacaat gccaaaacta
     6301 gcagaccgca tgtgggtatt ggtaaaagcc atatctctgt cccctttctt ttcccttatc
     6361 atacagcaaa taggattaaa aatcaagaaa aggtgatttt ttgaaaagga ttttaactac
     6421 aaggaaaaag aaggaaaaaa attcccaaaa atctaccgaa gttaataggt aaattcccaa
     6481 attaacttga ttatataact ttcagtta
//
LOCUS       DAVGJJ010000092         6414 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000092 DAVGJJ010000000
VERSION     DAVGJJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.092"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..961
                     /locus_tag="VVE35_001774"
                     /pseudo
     CDS             1..961
                     /locus_tag="VVE35_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1195..1491)
                     /locus_tag="VVE35_001775"
     CDS             complement(1195..1491)
                     /locus_tag="VVE35_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061918.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDTIDEAKSQITFK"
     gene            complement(1649..1936)
                     /locus_tag="VVE35_001776"
     CDS             complement(1649..1936)
                     /locus_tag="VVE35_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061919.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDILQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            complement(2730..2867)
                     /locus_tag="VVE35_001777"
                     /pseudo
     CDS             complement(2730..2867)
                     /locus_tag="VVE35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            complement(3069..3656)
                     /locus_tag="VVE35_001778"
     CDS             complement(3069..3656)
                     /locus_tag="VVE35_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU3061920.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKSRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(3659..3892)
                     /locus_tag="VVE35_001779"
     CDS             complement(3659..3892)
                     /locus_tag="VVE35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU3061921.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            complement(3905..4159)
                     /locus_tag="VVE35_001780"
     CDS             complement(3905..4159)
                     /locus_tag="VVE35_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061922.1"
                     /translation="MMSYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRL
                     PLAVSNKRVYPSLNLEEARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(4279..4602)
                     /locus_tag="VVE35_001781"
     CDS             complement(4279..4602)
                     /locus_tag="VVE35_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061923.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMELNLW
                     EVSYD"
     gene            complement(4616..4864)
                     /locus_tag="VVE35_001782"
                     /pseudo
     CDS             complement(4616..4864)
                     /locus_tag="VVE35_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(4864..5034)
                     /locus_tag="VVE35_001783"
     CDS             complement(4864..5034)
                     /locus_tag="VVE35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061924.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            complement(5221..5589)
                     /gene="rplL"
                     /locus_tag="VVE35_001784"
     CDS             complement(5221..5589)
                     /gene="rplL"
                     /locus_tag="VVE35_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU3061925.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(5665..6165)
                     /locus_tag="VVE35_001785"
     CDS             complement(5665..6165)
                     /locus_tag="VVE35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU3061926.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(6204..6339)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         2061 a         1290 c         1030 g         2033 t
ORIGIN      
        1 cgttctattt cgtctgatgg gcaaatctta taaagagatt atagagcttt tagactgtaa
       61 ttaaacaacg atttagcaaa atgtaaaaaa atatgaggag ttcggactcg actctctcct
      121 tcaagaaaca cgtggtggtc gtaaccatgc atatatgaca gttgaggaag agaaagtctt
      181 tcttgcccgc catttgaagg ctacagaggc aggagaattt gttacaattg atgccttatt
      241 tcaggcttat aaaaaggagt taggtcgttc ctacacacgt gatgccttct atcaactgtt
      301 gaagcgccat ggctggcgaa atattatgcc acgtccagaa catcctaaga aagcagacgc
      361 tcaaaccatt gtcgcgtcta aaaataaaat ctcaattcaa gaagataaga aagcgattta
      421 aaaccagtag acgttttcat aaggttcgct tgatgtacca agatgaggct ggtttcggta
      481 gaatcagtaa actgggatct tgttggtctc caataggagt aggtccacat gtccatagtc
      541 actatatacg agaatttcac tattgttatg gagctgttga tgcccataca ggcgaatcat
      601 ttttcttaat agctggtgga tgtaatactg agtggatgaa cgccttttta gaagagcttt
      661 cacaagctta tccagatgat tatcttttac tcgttatgga caatgctata tggcataaat
      721 caagtacctt aaagattccg actaatattg gttttacctt tattcctcca tacacaccag
      781 agatgaaccc attgaacaag tgtggaaaga gattcgtaaa cgtggattta agaataaggc
      841 ctttcgaact ttggaagatg tcatgaatca acttcaagat gtcatacaag gactggagaa
      901 ggaggtgata aagtccatcg ttaatcggag atggactaga atgctttttg aaaacagatg
      961 agtattagaa ataaatgaaa agtcaatatc aaaacaatca gaatcttgat gatgctaaat
     1021 tcaaaaaaac tacataataa ttttaataag ggtaagtcgt accaagaaac cccaccaaga
     1081 taaaaactta gtggggtttg gcggggaata taattattct cccaattatt atcgccttta
     1141 tattatctca actctcatta aacaagagtt ccgcaatcaa attgcgatac tctattattt
     1201 aaaggtaatt tgggatttag cctcatcaat tgtatcgtct aatgacttat ccatggaaaa
     1261 atcagataat ttctcaggat cattcaccat tttttctaac agatggtcta aatgattgtc
     1321 tagaatcgaa cggtctttcg tgtagaaatg cagagaatcc ccttgccaag cgatggctaa
     1381 aggaatctct tctttttcta aaaaagcttt aaatttctct atatcaattg gtttttctaa
     1441 aaaatctttt ttcagattaa tcgtatcaat cgcataggga gattgtagca attcttctaa
     1501 tttctacacc cctatcttat aggcggaatc ctgttctaaa gcctgacatc tagaccattc
     1561 tagtatcttt aaaagacctt ttacagtaaa taaaagacta cgctcagcat attgaactgc
     1621 cattcgttcc tgttgttcag aggacatctc agacctcctt cttaacaaat agcagctttc
     1681 cttctttata acgataagct atcaatttct gacgttgctt aatcgacttg acaacctgca
     1741 gtatatctct cgaataaggt tctcgaagag taacttctac tgctttccca tcaaagatac
     1801 caacacgttt aaattcaatg tagtcctttt tgagatgtcc taagcccata ccaaaaggaa
     1861 agtgatgaat caattggatg gtacaaccac ctttatttcc catttgtttc aaatcataca
     1921 agtttactac cttcatgatt aactccttta tctagtttgt cgcatcgttt gagtctggaa
     1981 acgataaact ccgtgtctgt tagaaaattc tcacacacgt cttgtgccag ttgcccttca
     2041 cagggaaata ctctcagtcc ctacttacac aggcacgcta atcaagacgg agtggattca
     2101 attttcaaag aacaggtagc tttattatag ataagagtag ttgaaatttt tatcacattt
     2161 tttatttttc tgtagagcta aattcatact aatcaaagag tttgaaataa atttgagtat
     2221 tattaactaa cgttcttcgt ctaaaaatac agtagggaat atttcattct tttatttaaa
     2281 attgtctaaa ttcgacagaa ggaagtagca agcattgtcg ctaaaaaaat gcatcaggtt
     2341 atcctgtgca tttttttgat atcaaaataa tcaatctcct ttatgagcta attcttctct
     2401 acaagctctt tggagcagaa caactgtaag tataaaaacc acacataaaa tcaagatact
     2461 tctctcagcc ccaaaaacac caccagtata taaatcttga cccgttaaat ttgtactgaa
     2521 taaactaaat gaaactttta tcccactcac taatactatt atatcaaccc actacagttg
     2581 acaaaaagac aaactagcct aatgactagc ttgtcttcaa tcaagagagg attctgttcc
     2641 ccccatataa gcactaacat tccaacgtgc acatattgga acgagatcca taactccaga
     2701 gaatctctaa agtttacaat ttaaatgaat taacaatttt cccaactaaa agcactccag
     2761 ttaccgcaac ggtttgtact gaatgtacta aatcgcattc catcaacttc atctgtttcg
     2821 tcaacttgaa cagatactaa ttaaagattt aatacttctt ctgccatagc taactcctcc
     2881 tatttaaatt tttgggatta agtgctttat ccacattcat tatactctct ccagcagtaa
     2941 aatgcaagca atgatacaat gttgtcacat agaaaataat gtttccgtaa cttttcaaag
     3001 taacttccat ctctctccca aaactggaag ttagttttag aagttaccta aaaatcaggt
     3061 tacctatttt aaaaaagcag caaactataa actagtaggt tccacaccaa atgtagcccc
     3121 atactgcccc ataagtccaa tttgtagcgt acaagcccta aaaacatccc cagcgataca
     3181 tacaaacacc aagctataat ggttcctgga tgatgtgcta aggcaaataa aacacttgtc
     3241 aaagcaactc gaatatctaa ttttctaacc aagttccata aaatttctct atacagaaat
     3301 tcttcaacca tactcgcatt gattaagaac aataaaaatg aaaaccaagg aacttgatat
     3361 tgaagcccaa ttaagtttgc ttgattcgtg ctttcttgcg catggattag actaaaacat
     3421 agacttataa tcagtagact ggctaatcca ataccaatcc attttatcct tgatttcata
     3481 ttgaccttat gcgcttgttt acgttgggca tacatccata aaaaagaaat gagtgacgca
     3541 caatatagaa tctgtagtat agttaactca ccgatacaaa gaaatttcag taagtataga
     3601 gataccaata ggacatttac ttgttggaat atataaactg gaattattct tttcatagtt
     3661 acctccgaaa taaatcttca taatctaaat ctaatacctg cacaatcctt tctacccatg
     3721 gactttgagg cattcgttgt tccatcttgt agtgacgaat cttttgatac aaacgattca
     3781 attcacttgg atagtgaaac tctcccgcaa acatttttct ggttagctca atccagctga
     3841 tatttctttc agccaaaata atggacaagt tctcccaaaa tcgttcagcc atattgcttc
     3901 tcctttagtt agataaataa tgtgtttgcg ccatgtaaat caattgtttc gtatctcttg
     3961 gcaatagagc tctagcctct tccaaattca gacttggata aactcgctta tttgaaaccg
     4021 caagaggaag tctgatggtt agttcaggat tttttaatat catttcgatg aaatctgtta
     4081 attttaggtt atctcgattc ttaaaacgta ataaatttgg agataaaaac tcaaaacaat
     4141 ctgaagaata gctcatcatc tcaattaatt tgtcctttgt catttcagaa actgaatgac
     4201 aagatacctc gatgccatag ttttggaaga aagtctaaaa gaagttgatt tcttcggcta
     4261 tttttactta gatagagatc aatcatagga gacctcccaa agattcagtt ccatttgata
     4321 ttctgacacg attaaggaat ctaataaatt tgcgaagtta atcggtttct tgtcttcatc
     4381 ataagctttt acagttactt gggttgtaag tattccctct tttccctcgg ctcgatagcc
     4441 ttgtccatat aaaacaaaaa cgagattttg atgatcatct acaaaggcat caaccccatt
     4501 ctttatgtct tgactttcaa ggaattccat aacgttttga agataggatt cgtaaaatag
     4561 tgggtagtta tgttttttat ggtaatcatc taaaaatgtc acttcaaact cacatggaga
     4621 gtaattttga ctttgaacag cctaaaagtg ccatcaaatt tgaattggaa taaatcaaat
     4681 aaatagtccc atcctcatca atccaacctt tgctcaaaga caactccaac cgatctttta
     4741 aaactgagta aaccacctta acctccggtt tcatattctt ataccgttca ctctcaaata
     4801 aaagtttggg gagcttataa taacgctctg atgtctgata ttgattagcg gtaatacgct
     4861 tcattattgt ccctccaaga ctaaaattcc aacatttcca aattcatcaa atcggattaa
     4921 acctacttgt tccatttcat caactaactg agttgctttt acaatatcaa ctcccatgat
     4981 agtcatgaga tgactcttca cgaattgacg tgatgactgt ccttcctttt gcataattac
     5041 ctccgaaaca caaaaaaagg ggtagacaat ctagtgtcta cccccgaaag tttattaaaa
     5101 caaaaatcct gccaaagaat ttttggcagg aaaccaaatc aatttatcag tttctatcaa
     5161 tcgcttatcg ctctcaaaga ctggtaaata gggattccgc aatcaaattg cgatactcta
     5221 ttatttaaga gtaactgaag ctccagcttc ttccaattta gctttgattt cttcagcttc
     5281 tgcagttgca acgccttctt taacaagtgc tggtgcaccg tcaacaagtt ctttagcttc
     5341 tttaagacca agaccagtga tttcacgtac aactttgata acgccaactt ttttgtcgcc
     5401 tgcagatgtc aattcaacgt cgaatgaatc tttagcagca ccagcatcag ctgcatcagc
     5461 tgcagcaaca gctacaggag cagctgcagt tacaccaaat tcttcttcga tagcttttac
     5521 aaggtcgttc aattcaagga ttgaagcttc tttaatttca gcaataatgt tttcaatgtt
     5581 caatgccatt gttatttcct ccaaataagt tttaaatttt ataatagttt ttttcgtagc
     5641 taggctacgc tgtgtagctt aagattaagc cgcgtcttct ttgctttctg caaccgcttt
     5701 gactgcaaga gcaacgttgc gcactggcgc ttgaagtaca gaaaggagca tagaaagaag
     5761 tccttcgcgg tttggaagag ttgcaagtgc aagaatctct tctttagatg cgacagcgcc
     5821 ttcgattgca ccacctttaa tttcaagtgc ttcagcgttt ttagaaaagt cgttcaagat
     5881 tttcgctggt gcgataacat cttcattaga aaatgctact gcagatggtc caacaaatac
     5941 agatgcaaga tcttcaagac cagctttttc agctgcacga cgcaagattg agtttttaat
     6001 aactttatac tcaacttcgc ttccacgaag ctcacgacga agaactgtat cttgctcaac
     6061 tgtcaaacca cgagcgtcta caacgacgat agatgcagca gctttcattt tttcagctac
     6121 tacgtcaact agttccgctt ttttagcaat aattgcttca ctcattagtg tgttcacctc
     6181 cgtaattatt ttgcttgggg aatttttcaa aaagaaaaac gcgcccaaac ctagacacga
     6241 aagtacaata cgcttctttt tacatgatac gtcttgtcct cggtaggata tttatgagtc
     6301 gagctcccct actgtcttag gcagtttttt taaaccgtat ataagtatag catagtcaaa
     6361 aaaagaatgc aagatttttg caaacttttt ttaaaatttt tcgtgatttt tctt
//
LOCUS       DAVGJJ010000093         6291 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000093 DAVGJJ010000000
VERSION     DAVGJJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.093"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(212..1762)
                     /locus_tag="VVE35_001786"
     CDS             complement(212..1762)
                     /locus_tag="VVE35_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU3061927.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1778..2557)
                     /locus_tag="VVE35_001787"
     CDS             complement(1778..2557)
                     /locus_tag="VVE35_001787"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU3061928.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2544..3395)
                     /gene="ylqF"
                     /locus_tag="VVE35_001788"
     CDS             complement(2544..3395)
                     /gene="ylqF"
                     /locus_tag="VVE35_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU3061929.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVIGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(3602..3778)
                     /locus_tag="VVE35_001789"
     CDS             complement(3602..3778)
                     /locus_tag="VVE35_001789"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU3061930.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(3830..>6291)
                     /locus_tag="VVE35_001790"
     CDS             complement(3830..>6291)
                     /locus_tag="VVE35_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU3061931.1"
                     /translation="NQLSTDHNLFKKELLSVVAMKNDEVVSDITSNKNSINHLLLHYK
                     DGTSEKVDLTYNSDFANLAEYNIENTGLVYTPNAFLKNYTPIIDRVKNDLTAVPYNPT
                     ALKNLLGISDNVKLTELYLDEQFTKTKEHLTETLKKLLSADAAVSGNNDIIDNYIVDK
                     IKRNKEALMLGLTYLERWYDFKFDKASAKDLLMFHMDFFGKGNTSPLDAIIELGKSGY
                     NNLLAKNNVVTYNALLTNNYGTKDLFSALEGYRKAFAPHQTNNEWFKSQTKAYIVEEK
                     SNIEEVKTKQGLVGTKYSIGVYDRITSATWKYRNMVLPLLTLPEKSVFVISTISSLGF
                     GAYDRYRNSEHKAGKDLNDFVEENARETAKRQRDHYDYWYRILDDNAREKLYRNILLY
                     DAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVGNKVGHNQHGAYATGDAVYYMGYR
                     MLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDHPDDATITINS
                     ILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVHNMFDVVYMLEYLEGNSILKLDTN
                     QKQQLLRKVTNEYHPDPDGNKVYATNVVRNITVEEVERLRSFNDLIDNNILSSREYAS
                     GKYERNGYFTIKLFAPIYAALSNDDGTPGDLMGRRMAYELLAAKGFKDGMVPYISNQF
                     EADARANNKTITSYGKTKGLVTDTLVLQKLFNGQYNTWSDFKKAMYKERQDKFNKLNK
                     ISFKDPSQPWTRNIIKTIHSVNELQNLMNEAVRKDTETPHWYNYNPEIDSAVHKLKRA
                     IFKAYLDQTNDFRSSIFENKK"
BASE COUNT         1844 a         1248 c         1144 g         2055 t
ORIGIN      
        1 actgtacaga tttgatacag taaactcctt tctagcttta tgctttatca tttctaactt
       61 aattaattat agtcttattt ttaataaagt tcaattattt atatgtaaaa ttcctcatca
      121 gtaaaaaata acctatctca tttctgagaa tcggttttct aaataaatca agctgagcat
      181 ttacataatt tatgttatgt aaatgctcat actacaataa caaatctttg acttttccaa
      241 tttcactttt tggaataacc aggtgaatca tatcacccag atacattctg gttgagccgt
      301 taactgtttg gctcttgcca ttatggactt gagttgtgat gaggacgttg tgtggtaagt
      361 tgagttcatg aacttgtttc ccagcaattt tatcagaaac tggtatttcg ataagtgtaa
      421 cttctccttc gctagatact tcttctggaa gcattttttc cagcatggct tcatagactg
      481 gcgtaccttt gagcaaatcc atgataatat aagaaacaag agtgacaaga ccaagtggca
      541 tgaggttgcg aatatctcct accatctcag ttacgaggat catagcggtt aagggagctt
      601 ttgatatggc tccaaaatag ccactcattc ctagaatgac aaatataggg aattgctctt
      661 gactgacaag tccaagattg acacagataa caccaactaa ggcaccaagc aaagaaccaa
      721 gagctaaaat ggggaggaaa attcctcctg gcagtccact tccatagcta atcatactcc
      781 aaataaagcg gatcaaaaag taagctaata aaacttggaa actaaaattt tgttcagtta
      841 aagaaaggac aagctgattt ccgccaccaa tgatttgagg taagaagatt ccgactggta
      901 tgataaggat aaaagccaag atgggataat aagccctatc caaatggatt ttttgaccaa
      961 tcaagtcata aactcttcca acgtttaata cagctttctc atagagaaaa cctgaaaatc
     1021 caaggaaaat tcccatgacg agatatatcc aatactggtc tagggtcatg ggaggaatgt
     1081 tatctggcat atccaatact ggtgtcaaac cgaacatgag tagagacaca aagtttgcta
     1141 cgatgctggc tgctagagtt gagacccaga aaaagcgcga aaaatggtga tagacttctt
     1201 ctacaacaaa gagaagtgct gcaataggag cattaaaggc tgcggctaaa cctgctgcag
     1261 ctccgctagc aatcaaggaa cgttcctcta ctggactgga tttgagccac ttggcaattc
     1321 ctttaccacc aactgctcca agttgaatgc tgggtccctc tcgacccagc atgagtccac
     1381 tggcaatagc aagaatacct agcacatatt ttttccaaag aatgccccac cagttgaggg
     1441 acatgaggcc tttcagttcg gcttcgactt gaggaattcc tgagccttta atatcttttt
     1501 ctgaccgtgt tagtttggca ctgagccaac agatgagtat ataaaacaaa accagtacaa
     1561 aaagattgcg cactaagtac ccttgatctt gataaactcc ttgtatcagg tggaagccct
     1621 tttcaattaa gaaacgaaag gatccgacga taattccaac gatgaggccg acaatgattc
     1681 ctcgaccaac ttgggatagt atagtgctgg atgcaaaggc gaattctttc ttagaacgga
     1741 ctatttccga ctgttcctcc atggtatttc ctttcaatta actttctttt ttacctaaaa
     1801 ccagtgattt aacgggttca aagctggttc ggtgaattgg ggtaactcct agttttgtga
     1861 ggccttccag atgtttagct gtgccatatc ctgcattagt agcgaaatca tagccaggga
     1921 actgctgatc gtattctttc atcaattcat cacgtgttac cttggctact atagatgctg
     1981 ctgcgataga gagggagttg gcatctcctt tgataatgga ggtttgtgaa atgggcaagt
     2041 ccagtttcat ggcatcaatc aaaaggtgct ctggttgagg gctgagctgg gagattgctt
     2101 cttgcatggc tagtttggtt gcttcataga tattgacttg gtcgatgacc tgattatcta
     2161 tgataccaat tccaatcgac aaggcttggt cttgaacggc ttggaaaatc tccagatgtt
     2221 tctttttagg aattttcttg ctgtcgttga gacctttaat cttacaattt ttaggtaaaa
     2281 taacggccgc agcgactaca ggaccagcaa gaggaccacg accaacctca tcaatacctg
     2341 caattaaggt caatccttgc ttataaagtt ctttttcata agaaagcatg gattccaagc
     2401 gcaaattttc atctaattca gcttgaatgg ctcttttacg cttgctgatt tccttttgaa
     2461 ctcctgagcg attatccttt tcaagctcta aaaaaatagg gctttctaac tccttgactg
     2521 tcacaaggaa ttctttgatt tctttaatcg ttgccatcga ggtcttccaa tgtatctaag
     2581 gtatagttac cgagtttgcc atcacggact tccttcacga agagactgta aaaacggtca
     2641 tagtcatcac ggaaaccgag ggcgcgggtc atatccataa taatcacagg cgcttcttct
     2701 tcaattttca tttgtttgaa gcgttcagcc agcttttctg gataatgttc tttgaaataa
     2761 ttgataccaa aaatggtaac ctcatccata ggaagcaact gatccttgat agctccagtc
     2821 aatgccaact taagtgcaac agtttcatcc tcaaacttag gccagagaat ccccggtgta
     2881 tccaagattt ccaggtcttt attggtttta agccattgtt gaccttttgt gacccctggc
     2941 ttgtttccaa taacagcaat ctttttacca gccaaacggt tcatcagagt tgatttacca
     3001 gcatttggaa tcccgataat catagtacgc aaggtttcaa tctgaatccc acgttctttc
     3061 tggcgagcaa tcttatcagc catgagcttc ttggccgcat ctgttacaac ttttacagtc
     3121 acttgctctt tggagttgat agctagcgtc tggattcctt gtgattcaaa atactgacgc
     3181 cattccttgg tcattgctgg atcagccaag tcggccttgt ttaaaatcaa gagttttggt
     3241 ttatcaccaa caatcttggt caacatagga ttttgactag atagaggcaa gcgtgcatct
     3301 actaaaatcg tcacaaaatc aacaaatttt aaattctcct gcacctgtcg acgagcttta
     3361 gacatgtgac caggaaacca ttgaatagta gccattgagt tttttccttt cgtagcaagg
     3421 atttagatcc cctattttat tttactatag tttgaacata gagagtcttc aaatggatga
     3481 tgtccaaaca gttcttgttt tatgacatag aaatctactt tatattatat catgccttct
     3541 ttattttgtc cgatgatagc cttttaccct atatagaaaa aagccatcaa atgatgactt
     3601 ttcagaggga gcaaaaaact gttcccgtca tattatgaac caaaagaact aaactaccaa
     3661 cagctaatag gcctaaagct gtaagcgcag aagttgatga acctgttgca ggaagagttt
     3721 cctgctgata aataagagca taacttactt cattatcagt aggaattcct gcttgaatct
     3781 tggtttgttc ttcatcacct ttttaaactt tatttcctaa tagtaaacac tattttttat
     3841 tctcaaaaat tgaacttcta aaatcatttg tttggtcaag ataggcttta aagattgctc
     3901 tcttcaactt atgaactgca ctgtctattt ctggattata gttataccaa tgaggagttt
     3961 ctgtatcctt gcggacagct tcattcatta agttctgcag ttcatttaca ctgtgaattg
     4021 tcttaataat attgcgcgtc caaggttgag acggatcttt aaatgaaatt ttgttcaatt
     4081 tattgaattt atcctgacgt tctttataca tagctttctt aaaatcactc caggtattgt
     4141 attgtccatt aaataatttt tgaagaacta atgtatctgt tactaatcct ttggtcttac
     4201 catatgaggt aatcgtttta ttatttgccc tagcatctgc ttcaaattga tttgagatat
     4261 atggcaccat accatcttta aagccttttg cagctaatag ttcataggcc atacgacgtc
     4321 ccatcagatc acctggcgtt ccgtcatcat tacttaatgc tgcataaatc ggcgcaaata
     4381 atttaatagt gaaatagcca tttctttcgt atttacctga ggcatattcc ctagacgaaa
     4441 gaatattatt atcaatcaaa tcattgaatg aacgtagtct ttcaacttct tctactgtta
     4501 tatttctgac aacatttgtt gcatagacct tatttccatc aggatcagga tggtactcat
     4561 ttgtaacttt tctaagaagt tgttgttttt gattcgtatc caatttaaga attgaatttc
     4621 cttcgagata ttccaacata taaacaacgt caaacatgtt gtggacatat tgcttcaaat
     4681 catctgcatt attaaatctt gtagttggat caagtacttg taatcgtcga ctttctgtac
     4741 tatcagattt cgaatgtttc aagatggagt tgatggtaat ggtcgcatca tctggatggt
     4801 ctggtgcttg taataatcct ttagcaaaga actctggtcc caagccactt cttcgaccat
     4861 atcctccaag ataaatgtcc tgatctgagt catgtgtcat ctcatgcgta taagtaatag
     4921 ctccatcctt atccaacatt cgataaccca tataataaac tgcatcacct gtagcataag
     4981 caccgtgttg attatgccca actttatttc caacaggtcc aaagaaatgt tgcattgcag
     5041 gatttggatt atcaaaatct gccacttctg tagctttccc tacggtatta tcatcgccaa
     5101 atttataagc atcgtaaagc aaaatatttc tataaagttt ttcacgtgca ttgtcgtcta
     5161 aaatacgata ccaataatcg tagtgatctc gctgacgttt ggctgtttca cgtgcatttt
     5221 cttcaacaaa atcattgagg tccttgcctg ctttgtgctc actattgcgg taacgatcat
     5281 aagctccaaa tcctagacta gagatggtcg agatgacaaa gactgatttc tccggcaagg
     5341 ttaggagcgg caagaccata ttgcggtatt tccatgtggc actagtgata cggtcataga
     5401 caccaataga atacttagtg ccaactagtc cttgcttcgt tttcacctct tcgatattgg
     5461 atttttcttc gacaatgtaa gccttagtct gtgatttaaa ccattcattg ttagtttgat
     5521 gcggtgcaaa tgctttacgg tagccttcta aagcactaaa cagatctttt gtaccgtagt
     5581 tattcgttaa caaggcattg taagtaacca cattattttt ggccaagaga ttgttgtagc
     5641 cagatttacc taattcgata attgcatcta gcggagaggt atttccctta ccaaagaaat
     5701 ccatatggaa cataagcaaa tcttttgcgc tagccttatc gaatttgaaa tcataccagc
     5761 gttctagata ggttaatcct agcatcaagg cttctttgtt tcgcttgatt ttatcaacaa
     5821 tgtaattgtc aattatatca ttatttccag aaacagcagc atcagctgaa agtaattttt
     5881 taagagtttc agttaagtgc tctttggtct tagtgaattg ctcatctaga taaagttctg
     5941 ttaatttaac attatcagag attcctaaca aattcttaag agcagttgga ttatacggta
     6001 ctgcagttaa atcattttta actctgtcta ttataggcgt ataatttttt aggaaagcat
     6061 ttggtgtgta tactagacca gtattttcaa tattatactc agccagattt gcaaagtcac
     6121 tattgtaggt taaatctact ttctcagatg ttccgtcttt gtaatgtagt agtaagtgat
     6181 tgatagagtt cttgtttgaa gtgatatctg agactacttc atcatttttc atagctacaa
     6241 cagacaacaa ttctttctta aacaggttat gatcagtaga taattgattt c
//
LOCUS       DAVGJJ010000094         6255 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000094 DAVGJJ010000000
VERSION     DAVGJJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.094"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(48..1619)
                     /gene="ffh"
                     /locus_tag="VVE35_001791"
     CDS             complement(48..1619)
                     /gene="ffh"
                     /locus_tag="VVE35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU3061932.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(1631..1963)
                     /locus_tag="VVE35_001792"
     CDS             complement(1631..1963)
                     /locus_tag="VVE35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU3061933.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(2054..2431)
                     /locus_tag="VVE35_001793"
     CDS             complement(2054..2431)
                     /locus_tag="VVE35_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU3061934.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2503..3807
                     /locus_tag="VVE35_001794"
     CDS             2503..3807
                     /locus_tag="VVE35_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU3061935.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIKNGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            3820..4626
                     /gene="yidA"
                     /locus_tag="VVE35_001795"
     CDS             3820..4626
                     /gene="yidA"
                     /locus_tag="VVE35_001795"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU3061936.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETDTGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYTTKTNDESGVAHAIRTWVL"
     gene            complement(4681..4752)
                     /locus_tag="VVE35_001796"
     tRNA            complement(4681..4752)
                     /locus_tag="VVE35_001796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4717..4719),aa:Arg,seq:cct)
     gene            complement(4794..5141)
                     /gene="rplS"
                     /locus_tag="VVE35_001797"
     CDS             complement(4794..5141)
                     /gene="rplS"
                     /locus_tag="VVE35_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU3061937.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(5260..5589)
                     /gene="crcB"
                     /locus_tag="VVE35_001798"
     CDS             complement(5260..5589)
                     /gene="crcB"
                     /locus_tag="VVE35_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU3061938.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(5583..5957)
                     /gene="crcB"
                     /locus_tag="VVE35_001799"
     CDS             complement(5583..5957)
                     /gene="crcB"
                     /locus_tag="VVE35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU3061939.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDAVKLLDTGRYLSLI
                     LYLLLTIGGGLLLAYYLGRKKW"
     gene            complement(5954..6220)
                     /locus_tag="VVE35_001800"
     CDS             complement(5954..6220)
                     /locus_tag="VVE35_001800"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU3061940.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
BASE COUNT         1851 a         1482 c         1136 g         1786 t
ORIGIN      
        1 ttttacttta ttttctcatc aaatgagatt tgcatcaatc tcttgtctta cttgcgtttc
       61 ttcttcgctt tcttcatttt gttagccata cgtttcatgg actgtttcat ggcaaattca
      121 ccaattttac ctttcaaacc gccaccaaac atctggctca tatctggcat tcctgctcct
      181 ccgagagctg ataagtcagg cataccgcct tgtcccatca ttccttcaag ggcagacata
      241 tccattcctc ccatatttgg catattttta ggaaggttat ttggattaat ccccatttgc
      301 ttcatcattt tattcatatc cccagacata acaccctgca tgagctgttt agcctggtta
      361 aagtccttga tgaatttatt gacttcgacg aatgtatttc cagaaccagc agcaatacga
      421 cggcgacggc ttggatttaa caaatctggg ttttcacgtt cttcaggtgt catcgaagac
      481 acaatggcac gtttacgagc aatctggcgt tcatccacct tcatgttttg aagtgctgga
      541 ttgttggcca tacctggaat catcttgagc aagtcttcca tcggccccat attttgcacc
      601 tgatctaatt gatcgatgaa atcattaaaa tcaaaggtgt tttcgcgcat cttctcagcc
      661 atttcaaggg ctttttgttc atcgtattcc tgagaagctt tctcaatcaa agtgagcata
      721 tcccccatgc caaggatacg gctagacata cggtctgggt ggaaggtttc gatatctgta
      781 attttttcac ctgtaccagt gaacttgatt ggttttccag tgatgtgacg aacagacaga
      841 gcagcaccac cacgagtatc accatcaatc ttggtaagga tgaccccagt cacttccaac
      901 tgagcattaa actcacgcgc aacattggct gcttcctgac caatcatagc atcaacgaca
      961 agcaagattt catttggttg agccaatact ttcacatcac gaagctcatt catgaggagc
     1021 tcatcaatct gcaaacgacc cgcagtatca atcaagacat agtcgttatg attagtttgg
     1081 gcttgctcca aaccttgacg tacaatctca acagctggta cttctgttcc aagtgcaaag
     1141 acaggcacat caatctgttg tcccaaggtc ttaagctggt caatggcagc tggacgataa
     1201 atatccgccg caatcatcaa aggacgagca ttttcttctt tcttgagttt gttggccaat
     1261 ttaccagcaa aggttgtttt accagcccct tgtaaaccaa ccatcatgat gatggttgga
     1321 atcttaggtg acttgataat ttctgccgta tcagaaccta aaacggctgt cagttcctca
     1381 tcaacgattt taataatctg ttgcgcagga ttaagtgtat caatgacctc atgcccgact
     1441 gcacgctcac gaactttctt gataaagtcc tttacaacag gcaaggcaac gtcggcctcg
     1501 agcaaggcca agcgaatttc tttggttgcc tcttggacat cagattcaga gatttttcct
     1561 tttttacgta gatttttaaa gacgttctgc aaacgttctg ttaaactttc aaatgccatt
     1621 tttcttcctc ttattctcta ttatcaatgc ttgttaaaat ttctatctgc tcctgcagaa
     1681 agtcatcctt cggatagcgc tccaaaatct gatcaaaaat ctgactgcgg acaatatagt
     1741 ccgagtacat gtgcaatttc atctcataat cttccagaat cttttctgtt cgcttgatat
     1801 tgtcatagac agcctgacga ctgacaccga actcctcggc aatttcagca aggctgtaat
     1861 catcagcgta gtagagctcg atataattca tttgcttatc tgtcaaaagc gccgcataaa
     1921 attcaaagag cgcattcata cgattggttt tttcgatttc cataactttt attataccaa
     1981 aaattagcct aatctaccac actaggaagc cgatccaaga agatagatag ctaaatttga
     2041 aaaagacatg agcctagccc caagtaattt ccaattgata gctggcaaaa ggatgtccct
     2101 cttgattttg tagttgataa tctagttcaa tcttttgccc atccacttga taacgattcg
     2161 tttggataat aaactcctgc atacccatag gtgtgggaat ataggctaaa ctatcactat
     2221 cctttagaaa gcgcataatg gtcttgggat tggaaaatcg actcatcaca agttcttgac
     2281 catgaaattt aatcactact ttttcctttt cctcattata gaaaagcagg tagctataat
     2341 ctcctttttc atgcacttcc acatcataaa gctggtcaat cacttccaac tgctcatcaa
     2401 actgaattgt atttcgcatc cgaatcttca catcaggccc tctttcttgt ttcttgtcct
     2461 actattttac caaaaagagc aggattttgc tataatggtc atatgaacga aaaagtattc
     2521 cgtgaccctg ttcacaacta catccatgtc aataatcaaa tcatctatga cttgattaat
     2581 acaaaagaat ttcagcgttt gcgccggatc aaacaactgg gaacttccag ttataccttc
     2641 cacggtggag aacacagtcg cttctctcac tgtctaggag tctatgaaat tgcacgacgc
     2701 atcacagaga ttttcgaaga aaaatatcct gaggaatgga atcctgccga gtctctcttg
     2761 accatgaccg ctgctctcct acacgacctt gggcatggtg cctactccca tacttttgaa
     2821 aatctctttg atacagacca tgaagccatt actcaagaga ttattcaaaa tcctgagaca
     2881 gagattcacc aagtcctact acaagtggca cctgatttcc cagaaaaggt ggccagtgtc
     2941 attgaccata cctatcctaa taagcaggtc gtgcagctca tttctagtca gattgacgca
     3001 gatcgcatgg actatctctt gcgcgactcc tattttacag gagcatccta tggggaattt
     3061 gacctgactc gaatcctccg agtcattcgt cctatcaaaa atggtatcgc ctttcagcgc
     3121 aatggcatgc acgccatcga agactacgtc ctcagtcgct accagatgta catgcaggtt
     3181 tatttccacc ccgcaacacg cgccatggaa gttctcctac agaatcttct caaacgcgcc
     3241 aaggaactct atcctgagga caaggatttc tttgcccgaa cttctccaca cctcctgcct
     3301 ttcttcgaaa aaaatgtgac cttgactgac tatctggctc tggatgatgg cgtgatgaat
     3361 acctacttcc agctttggat gaccagtcct gacaagattc ttgcagattt atcgcatcgc
     3421 tttgtcaacc gcaaggtctt taaatccatt accttttcac aagaggacca agatcaactt
     3481 actagcatga gaaaattggt tgaggatatc ggctttgatc ccgactacta cactgccatt
     3541 cataagaact ttgacctccc ttatgatatc tatcgtcccg aatctgaaaa cccacggaca
     3601 cagattgaga ttttacaaaa aaatggagaa ctggccgaac tctctagcct gtctcctatc
     3661 gtccaatccc ttgctggaag tcgccacgga gataatcgct tttatttccc gaaagaaatg
     3721 ttagaccaaa acagcatctt cgcaagcatc acccagcaat ttttacactt gattgagaac
     3781 gatcatttta ccccaaataa aaactaaaag aggaaattta tgagtattaa actaatcgcc
     3841 gttgatatcg acggaaccct agtcaacagt cagaaagaaa tcacacctga agtcttttct
     3901 gccatccaag acgccaaaga agctggtgtc aaagttgtca ttgcaactgg ccgccccatc
     3961 gcaggtgttg ccaaacttct ggacgacttg cagttgagag accaaggtga ctatgtggtg
     4021 accttcaatg gtgcccttgt ccaagagact gatactggcc atgagattat cagcgaatcc
     4081 ttgacctatg aggattatct ggatatggaa tttctcagtc gcaagcttgg tgttcacatg
     4141 catgctatta ctaaggacgg tatctatacc gccaatcgca atatcggaac atacactgtg
     4201 cacgaatcaa ccctcgtcag catgcctatc ttctaccgca ctcctgaaga aatggctggc
     4261 aaggaaattg tcaagtgtat gttcatcgat gaaccagaaa tcctcgatgc tgcgattgaa
     4321 aaaataccag ccgaatttta cgagcactac tctatcaaca aatctgctcc tttctacctc
     4381 gaactcctta aaaagaatgt agacaagggt tcagctatta ctcacttagc tgaaaaacta
     4441 ggattaacca aagatgaaac catggctatc ggagacgaag aaaatgaccg tgccatgctg
     4501 gaagtcgttg gcaaccctgt agtcatggaa aacggaaatc cagaaatcaa aaaaatcgcc
     4561 aaatacacca ccaaaacaaa tgacgaatcc ggcgttgccc atgctattcg tacatgggta
     4621 ctgtaaaagg aaagaataga taaaaaccgc tcagctgagc ggtttttgat gtctaattac
     4681 tgccccctgc aggaatcgaa cctgcaacta ctccttagga gggagttgtt atatccattg
     4741 aactaaggga gctagataaa aactctgctg aaaagcagag ttttttagtc gaattaacga
     4801 cggatttctt tgatacgagc tgctttacct tgaagagcac gcaagtagta caatttcgca
     4861 cgacgtactt taccgtaacg aacaacttcg attttttcaa cacgtggagt gtggattggg
     4921 aagatacgct caacacctac accgttagag attttacgaa ctgtgtagtt ttctgagatt
     4981 ccagcacctt tacgtgcgat aacaacacct tcaaaaatct ggatacgttc acggttacct
     5041 tcgacaactt tcgcgtgtac acgaacagtg tcaccaggac ggaatgatgg gatatctgta
     5101 cgaagttgac cttcagtcaa gctttggatt aatggattca ttttattctc ctatctttgt
     5161 caatcttgag gaaacttcct cagcggataa actgtatttt tgtgcgtcca tcacacacaa
     5221 gatacagtct accaaatttt cacaaaaaag taaagaaatt tatagcaaaa ttcctaaaaa
     5281 aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa gaataaaaga ccttcttatc
     5341 acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa gttgttaaac cgccacaaaa
     5401 tcctgttgct agaatagcat atacttcctt agactctaca tgattgtaga atactccaat
     5461 taaaaaacaa cctaaaagat tagctatgag cgttccaagt ggcaatttag aagcttgatt
     5521 gtagcgggaa aagaaatacc gtacgagtgc tcccaagcca caggcgattg caagatagac
     5581 gattaccatt tcttcctccc caaatagtaa gctaaaagca ggcctccacc gatagtcaaa
     5641 agcaaataca gtatcaaact aagataacgc cctgtatcca gtaatttcac agcatcaagc
     5701 atgagactag aaaaggttgt taaacctccg caaaaacccg tccccagtgc taaaatcaag
     5761 cccttactag tccccttata gaccagatag cccttgacaa gaaaaatcaa gcagaaaatt
     5821 cccagatagt tgacaaagag agtgccccaa ggaaagtctg gactggctgg taaccaggtg
     5881 gaaactagat atcggacaag tccgcccaac atagcagcta gaaaaattcc tagcggataa
     5941 aattgttctt ttttcatttg atattttgat cctgataatc acgcgaacgt ttgagtatgt
     6001 ccgaaaaagt cgctacaata gtctcctgat agcgcttgtc ctctacacga ctcctaacct
     6061 tttcaaaaat aacttcttct ctcttagtat ctaaaatcgg tttacctgaa gctttcttat
     6121 aagcaaccac cccctcaact aaatgcattc gttcttctaa cagtttgaca atttggtcgt
     6181 cgatttgatc gatttcttga cgaataatat ctaaatccat acagtctcct cctttatttg
     6241 aattattgta tcaaa
//
LOCUS       DAVGJJ010000095         6135 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000095 DAVGJJ010000000
VERSION     DAVGJJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.095"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            74..229
                     /gene="blpC"
                     /locus_tag="VVE35_001801"
     CDS             74..229
                     /gene="blpC"
                     /locus_tag="VVE35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU3061941.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(273..1613)
                     /locus_tag="VVE35_001802"
     CDS             complement(273..1613)
                     /locus_tag="VVE35_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3061942.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(1627..2364)
                     /locus_tag="VVE35_001803"
     CDS             complement(1627..2364)
                     /locus_tag="VVE35_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061943.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(2369..2707)
                     /locus_tag="VVE35_001804"
     CDS             complement(2369..2707)
                     /locus_tag="VVE35_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3061944.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2984..3313
                     /locus_tag="VVE35_001805"
     CDS             2984..3313
                     /locus_tag="VVE35_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU3061945.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3481..4530)
                     /locus_tag="VVE35_001806"
     CDS             complement(3481..4530)
                     /locus_tag="VVE35_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU3061946.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4527..5258)
                     /locus_tag="VVE35_001807"
     CDS             complement(4527..5258)
                     /locus_tag="VVE35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3061947.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTMQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5326..5736
                     /locus_tag="VVE35_001808"
     CDS             5326..5736
                     /locus_tag="VVE35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU3061948.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5746..6033
                     /locus_tag="VVE35_001809"
     CDS             5746..6033
                     /locus_tag="VVE35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061949.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         2032 a         1343 c         1120 g         1640 t
ORIGIN      
        1 taacccattc tgattaattt tcagataaca ctctataact atttattatc ttatcaaaaa
       61 ggagaatcat aacatggata agaaacaaaa cctaacttca tttcaagaac taacaactac
      121 cgaactcaac caaattatag gtggaggatg gtgggaagat ttcttatata gatttaatat
      181 aattgaacaa aaaaatacaa aaggatttca tcagccaata caactataaa aaaacaagac
      241 caagagaacg cactctcggt cttatttttt cttcattcta tatgtattac agtaagtacc
      301 tgacgaaaaa cgtgatcttg gcaggtggta ttgagactgg tattgggatg actttccaca
      361 attttcataa cggtatagag accaactcct ctctcctccc ctttagaact tgctccaaag
      421 gagaagattt cagaaatatc gattccctct tctttgatgg aattttcaat gataaaggtc
      481 tcctgtgctc catttttaaa aaaggcaatt gaaacatgag gttgacaggc ttctacacta
      541 gcttcaatag cgttgtcaca aaggatagac acaacggtta gaaaatcaag tagactcatc
      601 ccctctacct gaatctcctc tggaacttcg acattaaaga caatgttctt atctctagct
      661 tttataaatt ttccagctag gagacttttg agggcacgat cccgaacatt caccaatcgg
      721 ccaaggtcat atttattgtc ctgcaatttt tcactagaat cctttaagac cgagtcgtag
      781 atctctttta tctgctccat atcctcctct tcaatgccca gacgtaagct agtcaagagg
      841 ttggtgtagt catggcgaaa actccgtact tccttataaa gctcctctat atgccgacta
      901 taccgctcca tctctctgta gcgcaagtcc tgctcttggt tcagtctctc atggagtttg
      961 tccttcaaat aggtatccaa tttcttgata atccccataa aaaagagcag gtaaaagact
     1021 aggatgagat ggcgaacagt cgttgattga atcccctgtt catattcaaa gtaagacaga
     1081 ttttgtatca ccaaatagta agcccccatt atccagttaa tctgagtcag ggacttttga
     1141 aaatctttat cgagaatccc ctttctcaag ctagtgaaat catagtccaa ccatttcaaa
     1201 aaagctagag aaatgaagaa attgaaaatt attatacata accaaataaa tgagtagtca
     1261 tcatatactt gcccttgtcc caaaaatgga agcacaaaat aggaaacacc tctataaaag
     1321 agattcacta atatcattgg aaagagacca taaaagagaa ggagtttttt aggaagttct
     1381 cttaacaata agaaagataa gcctatgccg tacaagggtt ccataaaata agataggtaa
     1441 acatttccta ccatatagcc aagcattcca aaaacaacgg ccaacagtat cttaaaaaga
     1501 aaggccttaa aaatcctctc aaaagtgaga ctaattccat ctaccttaaa gaatatgaca
     1561 atttttaatc cgtgagtaac aagtgtatac aacaatatcc aagcaacatt cataaactct
     1621 cctagctcag tgtaagttat taatagcctc agacacttcc ctgaccttat aacgcgcgat
     1681 tagacaactt ccaccattgg gaaagaaaag cagtttttct ttcttatcta aatgcaccac
     1741 attggcagga ttgatgagaa aagagcgatg gcactgcaag agacgaggct cctgcttgag
     1801 aacctcctct agactcgctg taaattccag cctgtctgtc ttggtataga gaataacacg
     1861 atggggtctt gtggacgttt cgagatagta aacctcttta aaaggatact ggaattgagc
     1921 aaattttgat ttaaagtaaa agcaatcttc cgctagactt ttactgtctt gaccattggc
     1981 atagaggagg gctgtctcga tacgagattc aaactcctct gccgaaaggg ccttatcaat
     2041 atagtccaga gcagacactt ggtagcgaaa ggacaggggc ataaactccg agtgagtcgt
     2101 cacaaagaca atcagggcat acggatcccg atcccgaatc tttctagcca cttccagacc
     2161 cttcatctcc tcatttcgaa tctcaatatc caaaaagaat agctgatggg cccccttctc
     2221 atgcacctct gccagcaatt gatctggctt accaaaaact tcaaaagaac tcagagtgat
     2281 atgatgttcc ttcaagagtt tctcaatcgt cgtttcaatt ctagtctgtt gggaaaaatc
     2341 atcctctaaa acaaatattc tcatcttctt actctccctc tttcaaccat ttttgacgaa
     2401 tttctctata gcgacgtctg gcatacttga cagcgtattg accttcttct ccgagaaaaa
     2461 ggattctttc ttgaaaatca atcgatttta atttatcaag attaaccaaa caatttcgat
     2521 gacatctcat caaggtttcc ccatattggt tctcaagatt acttaaattt tgaagcatat
     2581 taaaactagc ttcttctgta acaatctgta cggtatgggc tttagttgga tgtgtttgga
     2641 tatagtagat atcgtcgacg tttactttat agactgtttt ctgcgtctga ataatcatat
     2701 gtttcattgg attctcctcc taagaaaaga ataacagaaa agttctagcc caagcccacc
     2761 caatcctaaa cagtctttga aagctcctaa aaggattatt tttctacaac atcatgatga
     2821 gcttacaact attttatcat aaaattcact cagcgcaatt caagacattt caatgacaat
     2881 taaagatttg aataaaaaaa aagaattaaa aatgataaac taacagtatc aaaacacagt
     2941 tgccagaagt aagactggca cccagattaa aggaggcatt cccatgacag atacagaccc
     3001 catcaaaaga gctcatactt tgattactga cttaaacaaa gcctaccaag catgcaaaca
     3061 ggcaagcgct gacgacgtcc gctttcagga gcaattaaac tctattcttg gttttttagc
     3121 caaggctgaa acagtggata atcgattctt gattgaacta gaaaaatttt accagacttc
     3181 cagtcttctc atgggactca gcgctcttga tccagatgct ccaactcgcg ccgcttggcg
     3241 ggcctatgac cgcttccact ttgaccaagt caagaccaag ttaatactca atgaaaatca
     3301 aagagcaaac taggaagcta gccgcaagct gtacttgagt acggtaaggc gacgctgacg
     3361 tggtttgaag agattttcga agagtataag tctctacgga ccaaccatta tcctgtagta
     3421 acaaaaaaga agctgagaca gactgaactc aacttctttt ttagtgtcgt atcagcaatg
     3481 ttagtcctgc atctgacgtt ttacctgata aggcaaatac aagactagta aaaccaaacc
     3541 agctcctagt aaggctagaa gggccagttt ttcttggaag gtaatcaacc caacaactaa
     3601 ttccacaagt aaaacaaaac tggtcaatcc tcgaactacc tcctgtaagc ctttttcctt
     3661 ttgccccttg tccagcggaa agagttgggt caaatactgg tagtcaaagg catgatagag
     3721 ggccagcaac tggaagagca agaggtagtt aaagagaact accactgctg tcgcaatcca
     3781 agcttgctcg ataaaaacct gcgccagtac ggaaagtaag agaagacgaa gactgagagc
     3841 aaagaggtcg ccatttcgca gataagaacg cagatagaga ttttgccaaa tcttcccagg
     3901 caccttctga acagccttta aaataaagtc cagataggca cgacgcttaa cgctgtttga
     3961 aattcccttg acctgcgtaa agagggcaaa gaaacgaagc aagacttgct tacgcttgct
     4021 ttcttgagaa ataagatagt cccagtccag tccagtttca gtgaaaaatt tgctggcctt
     4081 ttgacaaaag tggaaatatt ttcctacccc caataaaagc acatagagca gaaaaactgg
     4141 caagccataa cccattgcta aaaataaagg cgcaaataaa agcaagaaga gggtctggac
     4201 aaaaatccaa aagactaggg aaatgccagt ttgacgcttg agatggagct taatttcctc
     4261 ttctccaact aagagaaaga gcttgtctgg agcctccata taggtggcaa ttcctcccca
     4321 aagtaaaagt aaaacagacg taattcctac aaacaaaagg ataggccaat gattttcagg
     4381 aaaatgttgt aagagttgac tgtactggta ggctagaaag cccaacagga caagcaggaa
     4441 caagacaaag tggtcattga gcacatagcg cagataacca agacactcct tacgaaaggc
     4501 ctgctttctc tttaaaaaca agtctttcat aggtcctcct cttttgtcag agccaagtaa
     4561 atatcattca aactagcctc aggcatatca aaggcttcgc gtagttgcag gagattgcct
     4621 ttggcacgca cctctccctt gtgaagaatg acaaaggcat cacacatctt ctccgccgaa
     4681 tccagcacgt gggtactcat gagaatagac ttgccctttt gcttctccac ttccaaaagc
     4741 tgaatcaaat cagaaatagc tagcggatca agaccaagga aaggctcatc cacgatgaaa
     4801 agacttggat ccaccacaaa agcacagata atcatgacct tctgcttcat cccttttgaa
     4861 aaatgaacag ggaaccagtc taatttctgg tccagacgga acatctttaa caagggctct
     4921 actcgttcga aagccacttt ttgctcaata ccgtaagcca tagcaaccgt ttcgatatgc
     4981 tctctgaggg tcaattcctc atacagacta ggcgtctcag gaatgtagcc aatctgcttg
     5041 cggtagctag tcgcatctcc ttgcatagtc aggccattga tattgatgga gccactataa
     5101 ggtgccaaca gaccgataat ctcattgatc gtcgttgatt tcccagcacc attgagacca
     5161 atcaaaccga ccaactgccc actttcaaca gtaaaggaca catctttcaa aacaggaaca
     5221 tgaacatagc cacctgtcag gtttttaatt tctaacatat tttctccaaa tctggtataa
     5281 tgtagctata ttatatcaaa attcagtaca gtagaggtag attttatgtc agattgcatt
     5341 ttttgtaaaa tcatcgcagg ggaaattcct gcttcaaaag tatatgaaga tgagcaggtc
     5401 cttgcctttc ttgatatctc tcaagtaaca ctaggacaca ccttggtcgt gccaaaagaa
     5461 cactatcgca atcttttgga gatggatgct acgagcgcca gccaactctt tgcccaagta
     5521 ccaaaagtag ctcaaaaagt catgaaagtc actaaggctg ctggtatgaa tatcatttcc
     5581 aactgtgaag aagtcgctgg tcaaacagtt tttcatactc acgttcacct tgtgcctcgc
     5641 tacagtgctg acgatgacct caagattgat tttatcgccc acgaaccaga ctttgacaaa
     5701 cttgctcaag tcgctgaaac catcaaaaac gcttaaggag ttgctatgaa attatccaac
     5761 ctactgctat ttgcaggagc tgcagccgga agttatctgg ttacaaaaaa tcgccaaacc
     5821 atcacagatg aagtcttgaa taccactgac cgcgttcaag ctatcaagga cgatgtggat
     5881 attatccaaa acagcctgca aatcattaac cagcaaaaag aacttatcaa ggaataccaa
     5941 gaagacttga cttacaagtt taaggtcttg gaaaaggata tccagactag actagctgtg
     6001 ataaaagaaa tgcagggaac tgaagataag taaaaagagc cccccggctc ttttttaaaa
     6061 cgtaaacaag ttatagtgaa ttgaatttag aatagcacac catagtttct aaagcattgt
     6121 tagagattac tttaa
//
LOCUS       DAVGJJ010000096         6127 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000096 DAVGJJ010000000
VERSION     DAVGJJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.096"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            62..223
                     /locus_tag="VVE35_001810"
     CDS             62..223
                     /locus_tag="VVE35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061950.1"
                     /translation="MDNDWNGLADLIANLIVKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            315..1994
                     /locus_tag="VVE35_001811"
                     /pseudo
     CDS             315..1994
                     /locus_tag="VVE35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2262..2828
                     /locus_tag="VVE35_001812"
     CDS             2262..2828
                     /locus_tag="VVE35_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061951.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGVEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3150..3338
                     /locus_tag="VVE35_001813"
     CDS             3150..3338
                     /locus_tag="VVE35_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU3061952.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3400..4158
                     /locus_tag="VVE35_001814"
     CDS             3400..4158
                     /locus_tag="VVE35_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU3061953.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            4216..5070
                     /locus_tag="VVE35_001815"
     CDS             4216..5070
                     /locus_tag="VVE35_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU3061954.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5182..6063
                     /locus_tag="VVE35_001816"
     CDS             5182..6063
                     /locus_tag="VVE35_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU3061955.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKE"
BASE COUNT         2197 a          825 c         1300 g         1805 t
ORIGIN      
        1 atgtcaaaaa acttaattaa acaaataatt attagatata aaactttaga ggaggtatat
       61 attggacaat gactggaatg gattggctga tttaatagca aatctgattg taaaatatgc
      121 tggtgcttta gatttagata atctacccga tccaacgcca gcaaaaaatc aagagatgaa
      181 aaatagtttt gacatggcaa aaacacaaat tgagacggac taataaaagt gttataatat
      241 acttgaaatg atagtccaaa cttaaattaa aaaaattata tatgatatag aaaaattata
      301 ttgaggttaa tactatgtca aaagaaaaaa taaaagtata cctctataca cgagtatcta
      361 cgtcaataca gatagagggg tattcgttag aggcacaaaa atcacgaatg aaagcttttg
      421 ctatttacaa cgattatgaa attgttggag agtatgaaga tgccgggaag tctggcaagt
      481 ctattgaagg tagaatacag ttcaatcgaa tgatggaaga tataaaatct ggaaaagatg
      541 gagtatcctt tgttcttgtg tttaagttat caagatttgc aagaaatgcc gctgatgttc
      601 tttctacact tcagataatg caggattatg gagttaatct tatctgcgtt gaagatggca
      661 ttgattcatc taaagatgct gggaaactaa tgatttctgt tttatcagct gtggctgaaa
      721 tagaaagaga aaacatccgt attcaaacaa tggaaggtcg cattcaaaaa gcaagggaag
      781 gaaaatggaa tggtggtttt gctccgtatg gatataaact tgaagacggc aagctgttta
      841 taaatgagga agaggcagtt gcaataagaa cgattttcga ccagtatgta aatactacga
      901 taggagccaa tgggatatct aaatacttag agaatcatgg aattagaaaa atcccaagac
      961 agaatggtaa gaatcctttg tttgatgcag gtcttataag aaagatatta aagaatcctg
     1021 tatataatgg gaaaatagca tttggaagaa gaactttaga aaaagttcat ggtacaagaa
     1081 atgaatataa gcaggttgaa caagatgaat atctaatatc tgaagggata catgaagcta
     1141 tagtttccga tgaagtttgg caagctgctc aggttaagct aaaatctcaa gcaaagaaat
     1201 atgagcatgt gaataaagga aaagatacac gcacacactt gctttctgga attgtaaaat
     1261 gcccgatatg tggagtggga atgtttggga acaagtgtat caagaaaaag aaagatggca
     1321 caaagtataa agatttttat tactatggtt ataaacatag gcagatgata agaggtcata
     1381 agtgtacatt cagtaagcaa attagagaag aattgttaga tgatgctgtt gcagaggtga
     1441 ttgtcaagat agtaagtaat ccgaaatttg cttctatgat gcaagaaaaa atcaacatga
     1501 aggtggatac ctctgaaata gaaaaagaaa tagataatta ccaaaaggaa ttgagaaaga
     1561 gtcattctac aaagtttaag ctaattgagg aaatagataa tttagatgtt gaagataagc
     1621 attataagag aaggaaacag gatttagatg atagactata tcgtatgtat gacaaaatag
     1681 atgaattaga atcatcacta attgatgcga aagcaaagaa acagactatt gaagctgaaa
     1741 agcttacagg agataacata tataaggttc tgatctattt tgataagctc tataaagtca
     1801 tgaatgatgt agagcgtagg cagttaattt cagctttgat ttctgaaatt caagtttatg
     1861 aagaaaaata atctaacgga caatggctaa aatccattac ttttaagcta ccaattatag
     1921 aagaaaatct caacataggt ttggacaatg atgaacaagt tgaatgtgta agtttattag
     1981 agaaacgtag ttaatcctca aaaggttccc cgaacctttt gagtcccact gacgcatcac
     2041 gctgaggcgg tatcgctgtt tgtacgagtt gaagcatcag caaagtaaaa gtagatgttc
     2101 agccaatgcg acgaggtgag accgaaggtc gataaggtgt gttctgggta taggactcgt
     2161 aaagtaagta ggaaatatga tgagatgata cagagagcct gaattggtca ggtgacgtgc
     2221 ttatttacat tagcctgata atgaaaatgg agagattatc gatggttata ttggaattgt
     2281 accaaaatga ttattctaaa gatttagtag catttgattc catagaagat gggaaagcct
     2341 ttgtagctca aatcccggga tacactctgg aaacagaaga tggtgttgaa gtggagtatt
     2401 ttaatcctaa aaacataccg gattatatgg aaattatttt taacggaaat attgttcctt
     2461 tatctagatt tatgttcgat cctggggaaa atgtaaacat tatttggaaa gagatttcaa
     2521 atttatccct caaaaatgat agagtgatcg aaggatattc aaaaattgat gcatatgtcg
     2581 ttaacaacca tgaagttaaa gcctatgtcg aaacaagaga aacgaactat cgtaaagcaa
     2641 aagacttctt agaaagccgc ggatatgaaa tcgacagaag ctttttcgga agtgaagacg
     2701 gtgaagcgat tctttacaga aaaaaaggga tcgaagtttg gcatttcttg tgtcacttag
     2761 acccaatgtt tgtagagatt gaagatgtag aaggatatgt taaagaagaa gttggagaga
     2821 ttcaatagtc aaccaaagtt ccttgtgagt atataccttt gctagtaaaa gagtcaaaac
     2881 gttataacga agggggggaa gaaaaccctt gagtgtaagt ctttttcttg tttagaggga
     2941 aagtattaga gtagccgctc ataatttata ctagatttga taactgattt gagacagatt
     3001 ttatttctat aaatattgtg aaaatttttt aataccaaag atttaaagtt actgtgttaa
     3061 aaatcctctc cacttttaaa ctaatgtatt ataaaagcca gttgacacaa gtgttacacc
     3121 tgtgatagaa ttaacttgag gtgatgaaaa tggcaaccga taaaaataga ataatgatta
     3181 gcttggatga taaaaatcta gagaaacttg aaaatttagt agaagatgct agagatagaa
     3241 ggggaatgcg actaacaaag tcccaggtta ttgaattact cctaaatacc gttgattatt
     3301 tcgatgatat tatgggggct atttactcaa aaaaataaga gcaaaattgc tattattttt
     3361 tacaaattgg tgtaacacta gtgatatagg agtgatgaaa tggttgttta tattagacaa
     3421 agtaaattac caagtgaggt ttcaattaat aaataccatg ctcaagtagg tgcctactta
     3481 caaggagaag aagttatttt atatcaatct ttttctgaaa taaaagagtt aacaagtgaa
     3541 gatatagttg tagattatat aatggagact agagcattac taaaaatgat gggcttaaac
     3601 gttccggttt atgactatcc tattgagctt aaagagtttt atggtcgaaa gatttatgct
     3661 ggtattttag gagagattgt gaatatacct gataattggg gaaaatttat taagcctaaa
     3721 gctggttcaa aagtctttac tggaagagtt gttaatggaa cccatgattt aataggtatt
     3781 ggtctacctt tcgactatcc tatatggatt agtgaggttg tagaattcat agctgaatgg
     3841 cgttgttttg tgttagatgg tcgcgtatta gatgttcgac cctatacagg tgattatcat
     3901 gcacaatttg atgcaagtgt aattgatgaa gcgatatcat gttggaaaga tgcgccaata
     3961 gcttatggac tagatatcgg tgttactcgc gatggcagaa cacttgttgt tgaagtaaat
     4021 gatggttatg cattgggaaa ttatggccta tctcctttaa aatcaatcaa ttttcataga
     4081 gctaggtgga aagaaatggt aaaaccctat tttgaaaaaa atgaaatttt taaaatccaa
     4141 caggatgtta ttttctaaaa taaatatggt agataaccaa tataaaaagt atcaaaatga
     4201 acaaggagga atctgatgct tggagcaatt attggagata ttgtcggttc tgtttacgaa
     4261 tggaacaata tcaaaacaaa ggactttcct ttatttcgga aggactgctt ttttacagat
     4321 gacacggtta tgacctgtgc tgttgcagaa gcaattatga atggtggaca aaaagacgac
     4381 ttcattgacg cgatgaagaa atatggcaga atgtatccga atgctgatta cggtgctcgg
     4441 tttaatgcat ggctaaacag cgataaccgt gagccttata atagctttgg gaatggatca
     4501 gctatgcgta tttctccatg tgcttggatc atggactgtg gtttttatgc gaaaactggt
     4561 atatggccat catctagagg acttacgagt ctttctgcag aggtaactca taatcatcca
     4621 gaaggtgtca aaggcgctat ggctacagct gatgctatct ttctatgtcg tttttacttt
     4681 ggtggttatt gtagagagta cgagcaatca attaacgaca atcctacaga gtgtaaaaga
     4741 cgaattaagg attatataga aaaagaatac gattacaatc tatctcaaac tttagatgaa
     4801 atccgtccta actatcgttt taacgaaaca tgtcaggaaa ctgtccctca agccattatc
     4861 gcctttcttg aaagtagaga cttcgaagat gcgataagaa atgccatctc acttggtggc
     4921 gacagtgata ctctcgcagc aatcacttgt agcatagcag aggcagctta cggtattcct
     4981 gattggatca aggataaggc ctattcttac ttggatgaac ccttaaagga tgtagttagg
     5041 cgatgggaaa atagaataaa agcgtattaa tctccagtac gtcagatttc tcttttctgt
     5101 attttcattg tataatagga ttacatttac ttgcatacga cgaaatgtaa acatttctgt
     5161 ttaaaattaa ggataaaatc aatgaaaaaa gcaatggtaa ttatcaaccc tacttctggt
     5221 ggcgagaagg ctttggatta caaagaaaag ctggagaata aagcaaaaga atactttgaa
     5281 tatgttgaaa ccaaaattac cgaaaaagcg ctggatgcaa cacattttgc tgaagaagct
     5341 tctcgtgagc agtatgatgc agtggttgtg tttggtggag atggaactgt caatgaagtc
     5401 atttcaggta ttgatgagag agactacatt cctaagttag ggattatccc aggcggtacg
     5461 ggtaacctca ttacaaaact tttggaaatc aatcaagaca tcgatggcgc aattgacgaa
     5521 ctggattttg atttaaccaa taagattgat atcggtaaag caaatgacaa ctattttggt
     5581 tatatcttta gtatcggttc tctgcctgag gcgattcaca atgttgaaat agaggacaaa
     5641 acaaaattcg gtattttaac ctatgctgta aataccatga agtctgtcat gacagatcag
     5701 gtctttaaca ttaaggttga gacagaaaat ggaaattatg ttggtgaagc tagccatgtt
     5761 ttggtccttt tgacaaatta cttcgctgat aagaaaatct ttgaagaaaa caaggacggc
     5821 tatgccaaca ttttgattct gaaagatgcc tctatattct ccaaattatc cgtcattcct
     5881 gatttattaa aaggggatgt tgtcgcaaat gataatatcg agtatatcaa agcgcgtaat
     5941 attaaaatct cttcagatag tgaattggag tcagatgttg acggagataa atcagataac
     6001 ctacctgtag aaatcaaagt cctagctcag cgagtagaag tattttcaaa accgaaagag
     6061 taagtttgtc attcactgag aaataaagtt ttatatcaac gaaagtagtt acttgtaaga
     6121 tttgcta
//
LOCUS       DAVGJJ010000097         5937 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000097 DAVGJJ010000000
VERSION     DAVGJJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.097"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            53..820
                     /locus_tag="VVE35_001817"
     CDS             53..820
                     /locus_tag="VVE35_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU3061956.1"
                     /translation="MIGISLIPALYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSKKTTTLSNI
                     QSTAAYQSLTSEQQTGISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGAVTSKLVPASQLIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVS"
     gene            905..1243
                     /locus_tag="VVE35_001818"
     CDS             905..1243
                     /locus_tag="VVE35_001818"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061957.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            1271..1585
                     /locus_tag="VVE35_001819"
     CDS             1271..1585
                     /locus_tag="VVE35_001819"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU3061958.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYSLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1686..4238)
                     /locus_tag="VVE35_001820"
     CDS             complement(1686..4238)
                     /locus_tag="VVE35_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU3061959.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHMKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFMTGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4300..5922)
                     /locus_tag="VVE35_001821"
     CDS             complement(4300..5922)
                     /locus_tag="VVE35_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3061960.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIAQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1982 a          982 c         1103 g         1870 t
ORIGIN      
        1 ctatgtttaa agaatggaaa gcaatttaaa aaaaacaacc tttattattg tcatgatcgg
       61 gatttctctt attccagctc tgtacaatat catatttttg tcatcaatgt gggatccata
      121 tgggcaattg tctgacttac ctgtggcagt tgtaaataat gataaagagg cttcctataa
      181 tggtaatact atggcaatag gaaaagacat ggtgtccaat ttaaaagaaa ataaaacctt
      241 ggattttcat tttgtagatg aagaggaagg aaagaaggga ttggaagatg gcgattacta
      301 tatggtagtg actttaccaa gtgatttatc taaaaaaaca actacattat ccaatattca
      361 atcgacagca gcttatcaat cattgacaag tgagcaacaa actgggataa gtgattctgt
      421 atctcaaaat tcaactgata gtattcaatc ggctcagtca attgtagctt tagtacaagg
      481 tttacaggga agtttagaaa acttacaaaa tcaatcttct aatctttcga ctttaaaaaa
      541 tcaagctaat caagtattac ctattacttc tacttctttg ataggattgt caagtggatt
      601 aacagagata caaggagctg ttactagcaa attagttcct gccagtcagt tgattgcatc
      661 aggtgtaaac gcatatacta caggtgttga taaagtttct cagggcgcaa gtcaactaag
      721 tgaaaaaaat gccaccttga caggtagttt ggataaacta gtttcaggct caaacacctt
      781 gacacaaaaa tcttctagat tgacagcagg agttagttaa ttacaatcag gatctgggca
      841 attagcagac aaatccagtc agttactttc aggtgcttct ccattagaga atagagctaa
      901 taaattggca gatggatctg ggaaactagc agaaggtgga acaaagttaa cttctggatt
      961 ggaagattta cagacaggac ttgcttcttt aggacaagga ctaggtaatg ctagtgatca
     1021 actcaaatca gtatcaacag aatctaaaaa tgcagagatt ttgtcaaatc cactcaatct
     1081 ttcaaaaaca gacaatgatc aagttcctgt aaatggaatc gcaatagctc cttatatgat
     1141 atcagttgct ctttttgttg cagcaatatc aacaaatatg atatttgcga aattgccttc
     1201 aggacgtcat ccagagagcc gttgggcttg gttgaaatct tgagctgaaa taaatggtat
     1261 tatagctgtt ttggcaggaa ttttggtata tggaggagtt cagcttattg gtttaactgc
     1321 taatcatgag atgagaatat ttattctcat catcctaaca agtttagtat tcatgtctat
     1381 ggtgaccact ttagcaacgt ggaatagccg tataggagct tttttttcac ttattttgct
     1441 tttactacag ttagcatcaa gtgcaggtac ttattcactt gctttgacaa atgatttctt
     1501 tagatctatt aatccctggt taccaatgag ctattcagtt tcgggattac gacaaacaat
     1561 ctctatcaac aagtcatttt cctagctgtc atactagctc tatttactgg tttaggtatg
     1621 ctagcctatc aacataagaa aatggaagaa gattaaaaaa atcgaccgat taactggtcg
     1681 attttttatg ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa
     1741 gtaaaggaac agattgctcc agtcataaaa ccattgggaa tgaaggaaag tgtaatagtt
     1801 cctttcccct tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt
     1861 ttcttaccat cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat
     1921 gtatcttgtt ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca
     1981 ggttgttctt taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag
     2041 gtaggagatt caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt
     2101 tttttctctt tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc
     2161 tttttttcac catttacaag gatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg
     2221 tttatgaaag agagataaac ttggctgttt tctggaactt caatttgata ctggattgct
     2281 gcatcttcat ttgaagaact tgtgacacta atcaaatcat tagtattttc tattttttct
     2341 gttttttcat aaggtattgg agaaaaataa tcaaaattga cgttagcaag ttgatttaaa
     2401 aatgaggcct gattatccaa ggtatgttca ttgaacttga catcattgta aacagattga
     2461 ctagcaactg caatcggaag agagtattga ttttcatata gggtaagatt atctttttga
     2521 tagatatctt taaagccata cttatcaata ggactgtctg agatattgta ctggatacca
     2581 aataaactat cagccaaaat actattattt gcatatcgga gattgagatt agtcccagag
     2641 gatttaaaac caagtttatc taaagtagag cttgatgaac gatttcgaac agatgaaaat
     2701 tgagagattc cattgtagtt gaatttcata ctgtcatttc ctgtctgagt ttgtagtttt
     2761 tcagtacgag taaattgatt tccaatatat gttgagaaag attccatagc tgggatatct
     2821 cgactataag cacttcgaga agcaaatccc cattccttag caattccgtc catttgagat
     2881 gaagcattta aactcatttc aaccagtata aataaagaga ttagaatggc aaatagattc
     2941 acagatataa actttttgat aactgcaagg agtaaaagag aatagacaac caaaaattca
     3001 agagtaagca gaatattcaa atctgttaaa aaagaataat gcgattttag atagatggta
     3061 gctaaaaatc ctgctactac aagaaaaagc gaaactaaaa aattccagac tttaagttct
     3121 ttcagacgct ttaagacttc tgctgctgtg taaattaaca aggtagagaa aatccaagca
     3181 tagcgatgta aaaacatgtt tggagtatgc atgccttgcc aaaataagtc aagagcttct
     3241 atgtaaaagc ttgcaattag aaatgcaaag aatattacat atatgagttt catgtgaaac
     3301 ttaatagatt tcagcgtaaa aaataaaatg gtcaaaataa agggaaatag tccaacaaaa
     3361 atcattggga tggccccata ctttgttgtg tcaaaggaac caatgaattg cttagcaaag
     3421 agatcaagat accagctact ttcagtttga aactttgtaa cttcagtcaa tttttcccca
     3481 tgtgtctgta aatcaaatag agtgggaaga gtcataatca aactagccat accagctaaa
     3541 aaggagataa ctatgaaatc aagaacagat gattttcgag tcttaaagtc ccacgaaatt
     3601 tgacagagat accagaaaat aagaaacaat actgtcatat atccaaaata ataattttga
     3661 ataaataaga ttgacagact tgtaaagtac aataggagtt tcttttcagt tatcagtaga
     3721 tgtaaaccag ttataattaa aggaatcaag ataaaaacat ctagccaggt ttttatctct
     3781 aattgactga cagtgaaact catcagagca taggaagtag ataaggctag ttttaaagtc
     3841 tgagggatag attgaaacaa tttattcaaa ctaaaaaagg ttgacagacc aatcaatcca
     3901 aattttaaga gagtggtcag atagacagca tctggcatat tcgttagatc aaaaaagtaa
     3961 actagaggcg cgagaaaact acccaagtaa taactagata gggcatagaa gtttagccct
     4021 agaccacttg taaaggtgta aaacagacta ctatttccat gtaggatatt tcgtaaggct
     4081 acatcaaaaa taacgtattg atgaaagcca tctcctaata gaggagagtt gtcgctattc
     4141 cagtagatac cttgagatag atatactcca gacataatca ctacaggaat gatgaaagaa
     4201 ataaaatagg ttcgatatgt ttttaaaaat gatttcatgt tacctcgtag aatgatagaa
     4261 aactcagttg gttaacccaa ctgagttttg aagttttatt tagtctttcc aaagttcttt
     4321 aacttttgct tgtacttctg cattttctag gaattcatcg taggtttcat cgatacggtc
     4381 aatgacgcca tttttagata agacaatgat atggttagcc aaagtttgaa taaattcgtg
     4441 gtcatggcta gcaaagatga ttgattcttt aaagtttttc aatccatcat tcaagcttga
     4501 gatagattcc aagtccaagt gatttgttgg atcatcaagt acaaggacat ttgattttaa
     4561 gagcatgagt tttgaaagca tgacacgaac tttttctccc cctgacaaga catttacagg
     4621 tttgttaact tcatctccag agaagagcat acggccgagg aagccacgta ggaaagtatt
     4681 gtcatcttct tctttacttg cgaattgacg caaccagtca agaattgatt ctcctcctgc
     4741 aaaatcagct gagttatctt ttggtaggta agattgacta gttgtaactc cccacttgac
     4801 agttccttca tagtcaatat ctcccatgat tgcacgaatt aatgcagtcg tttgaatatc
     4861 attttgtgca ataagtgctg tcttatcatc tggacgcaag atgaaactaa tattatccaa
     4921 gatagtttca ccatcaatct ttacagttag attttctact gtcaagagat cattaccaat
     4981 ctcacgttcc gctttaaagt tgataaatgg atatttacga ctagatggca caatctcttc
     5041 tagctcaatc ttatcaagca ttttcttacg tgatgttgcc tgccttgact tagaagcatt
     5101 ggcagagaaa cgagcaacaa attcttgcaa ttgtttaatt ttttcttctg ctttagcatt
     5161 acggtctgct agcaatttag cagcaagctc agaagattcc ttccagaagt cgtagtttcc
     5221 gacatagagt ttgatttttc caaagtcaag gtcggccatg tgagtacaaa ctttgtttaa
     5281 gaagtgacgg tcgtgggata ctacgataac tgtgttatca aagtcaatta agaagtcttc
     5341 taaccaagta atcgattgga tatccaaacc gttagtaggc tcgtccaaga gaagaacatc
     5401 tggtttacca aaaagtgctt tggcgaggag aacctttact ttttcaccgt tggccaattc
     5461 gctcatgttt tggtagtgta attcttctgg aatgtttagg ttttgaagta gttgagaggc
     5521 ttcactctct gcttcccaac ctccaagttc ggcaaactct ccttcgagtt cggcagcacg
     5581 aaccccgtcc tcgtctgaga aatcttcctt catgtagata gcatctttct ctttcatgat
     5641 gctataaagt ttttcatttc ccatgataac gacatcaatg gcacgttcat cttcgtagtc
     5701 aaagtgattt tgacgaagaa cagagagacg ttcatctgga ccaagagaga tgtgaccagt
     5761 agtaggttcg atatctccag ctaaaatttt taaaaaggtt gattttccgg caccattagc
     5821 accgattaat ccgtaagtat ttccttctgt aaatttgata ttgacatcat caaaaagttt
     5881 gcgatcacta aaacgtagtg aaacatcaga tactgtaagc aatatttttc tcctata
//
LOCUS       DAVGJJ010000098         5918 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000098 DAVGJJ010000000
VERSION     DAVGJJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.098"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            128..1828
                     /locus_tag="VVE35_001822"
     CDS             128..1828
                     /locus_tag="VVE35_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU3061961.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1821..2297
                     /gene="ilvN"
                     /locus_tag="VVE35_001823"
     CDS             1821..2297
                     /gene="ilvN"
                     /locus_tag="VVE35_001823"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU3061962.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2363..3385
                     /gene="ilvC"
                     /locus_tag="VVE35_001824"
     CDS             2363..3385
                     /gene="ilvC"
                     /locus_tag="VVE35_001824"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU3061963.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3492..3728
                     /locus_tag="VVE35_001825"
     CDS             3492..3728
                     /locus_tag="VVE35_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061964.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3966..4322
                     /locus_tag="VVE35_001826"
     CDS             3966..4322
                     /locus_tag="VVE35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU3061965.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4372..5622
                     /gene="ilvA"
                     /locus_tag="VVE35_001827"
     CDS             4372..5622
                     /gene="ilvA"
                     /locus_tag="VVE35_001827"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU3061966.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1839 a          996 c         1348 g         1735 t
ORIGIN      
        1 caaaaataac attcataaat aaacgttaaa atagaaaatt cagaaaattt cttcttttat
       61 cttgaaaaat tttgaaaaaa tggtatgata gtaacaagtt atttttaaga ggaaagaaag
      121 gggaacaatg gagaaaatca gtttagaatc tcctaagacg gggtcggacc tagttttgga
      181 aacacttcgt gatttaggag ttgataccat ctttggttat cctggtggtg cggttttgcc
      241 tttttatgat gcgatatata attttaaagg cattcgccac attttagggc gccatgagca
      301 aggttgtttg catgaagctg aaggttatgc caaatcaact ggaaagttgg gtgttgccgt
      361 cgtcactagt ggaccaggag caacaaatgc cattacaggg attgcggatg ctatgagcga
      421 tagcgttccc cttttggtct ttacaggtca ggtggcgcga gcagggattg ggaaggatgc
      481 ctttcaggag gcagacatcg taggaattac catgccaatc actaagtaca attaccaagt
      541 tcgtgagaca gctgatattc cgcgtatcat tacggaagct gtccatatcg caactacagg
      601 ccgtccaggg ccagttgtaa ttgacctacc aaaagacata tctgctttag aaacagactt
      661 catttattca ccagaagtga atttaccaag ttatcagccg actcttgagc cgaatgatat
      721 gcaaatcaag aaaatcttga agcaattgtc caaggctaaa aagccagtct tgttagctgg
      781 tggtggaatt agttatgctg aggctgctac ggaactaaat gaatttgcag aacgctatca
      841 aattccagtg gtaaccagtc ttttgggaca aggaacgatt gcaacgagtc acccactctt
      901 tcttggaatg ggaggcatgc acgggtcatt cgcagcaaat attgccatga cggaagcgga
      961 ctttatgatt agtattggtt ctcgtttcga tgaccgtttg acggggaatc ctaagacttt
     1021 cgctaagaat gctaaggttg cccacattga tattgaccca gctgagattg gcaagattat
     1081 cagtgcagac attcctgtag ttggagatgc taagaaggcc ttgcaaatgt tgctagcaga
     1141 accaacagtt cacaacaata ctgaaaagtg gattgagaaa gtcactaaag acaagaatcg
     1201 tgttcgttct tatgacaaga aagagcgtgt ggttcaaccg caagcagtta ttgaacgaat
     1261 tggtgaattg acgaatggag atgccattgt ggtaacagac gttggtcaac accaaatgtg
     1321 gacagctcag tattatccct accaaaatga acgtcagtta gtgacttcag gtggtttggg
     1381 aacaatggga tttggaattc cagcagcaat cggtgctaaa attgctaacc cagataagga
     1441 agtagtcttg tttgttgggg atggtggttt ccaaatgacc aaccaggagt tggctatttt
     1501 gaatatttac aaggtgccaa tcaaggtggt tatgctgaac aatcattcac ttggaatggt
     1561 tcgccagtgg caggaatcct tctatgaagg cagaacatca gagtcggtct ttgataccct
     1621 tcctgatttc caattgatgg cgcaggctta tggtattaaa aactataagt ttgacaatcc
     1681 tgagaccttg gctcaagacc ttgaagtcat cactgaggat gttcctatgc taattgaggt
     1741 agatatttct cgtaaggaac aggtgttacc aatggtaccg gctggtaaga gtaatcatga
     1801 gatgttgggg gtgcagttcc atgcgtagaa tgttaacagc aaaactacaa aatcgttcag
     1861 gagtactcaa tcgctttaca ggtgtcctat ctcgtcgtca ggttaatatt gaaagcatct
     1921 ctgttggagc aacagaagat ccgaatgtat cgcgtatcac tattattatt gatgttgctt
     1981 ctcatgatga agtggagcaa atcatcaaac agctcaatcg tcagattgat gtgattcgca
     2041 ttcgagatat tacagacaag cctcatttgg agcgcgaggt gattttggtt aagatgtcag
     2101 cgccagctga gaagagagct gagattttag cgattattca acctttccgt gcaacagtgg
     2161 tagacgtagc gccaagctcg attaccattc agatgacggg aaatgcagaa aagagcgaag
     2221 ccctattgag agtcattcgc ccatacggta ttcgcaatat tgctcgaacg ggtgcaactg
     2281 gatttacccg cgattaaaaa tccaacttaa atttattaaa ccagcctaaa aggcaataaa
     2341 taatagaaaa gagagaaaag ctatgacagt tcaaatggaa tatgaaaaag atgttaaagt
     2401 agcagcactt gacggtaaaa aaatcgccgt tatcggttat ggttcacaag ggcatgcgca
     2461 tgctcaaaac ttgcgtgatt caggtcgtga cgttattata ggtgtacgtc caggtaaatc
     2521 ttttgataaa gcaaaagaag atggatttga tacttacaca gtagcagaag ctactaagtt
     2581 ggctgatgtt atcatgatct tggcgccaga cgaaattcaa caagaattgt acgaagcaga
     2641 aatcgctcca aacttggaag ctggaaacgc agttggattt gcccatggtt tcaacatcca
     2701 ctttgaattt atcaaagttc ctgcggatgt agatgtcttc atgtgtgctc ctaaaggacc
     2761 aggacacttg gtacgtcgta cttacgaaga aggatttggt gttccagctc tttatgcagt
     2821 ataccaagat gcaacaggaa atgctaaaaa cattgctatg gactggtgta aaggtgttgg
     2881 agcggctcgt gtaggtcttc ttgaaacaac ttacaaagaa gaaactgaag aagatttgtt
     2941 tggtgaacaa gctgtacttt gtggtggttt gactgccctt atcgaagcag gtttcgaagt
     3001 cttgacagaa gcaggttacg ctccagaatt ggcttacttt gaagttcttc acgaaatgaa
     3061 attgatcgtt gacttgatct acgaaggtgg attcaagaaa atgcgtcaat ctatttcaaa
     3121 cactgctgaa tacggtgact atgtatcagg tccacgtgta atcactgaac aagttaaaga
     3181 aaatatgaag gctgtcttgg cagacatcca aaatggtaaa tttgcaaatg actttgtaaa
     3241 tgactataaa gctggacgtc caaaattgac tgcttaccgt gaacaagcag ctaaccttga
     3301 aattgaaaaa gttggtgcag aattgcgtaa agcaatgcca ttcgttggta aaaacgacga
     3361 tgatgcattc aaaatctata actaattaga aatatatagc gctggagatg attttatgaa
     3421 aaagattatg agaaaaattg catcgttatt attggttcta gttgtataat gtaattacac
     3481 cgtcggtaat agtgctagca gaccaaaata aagcagattg gtcgtatgat gaaaatactg
     3541 taattaacat ttatgatgat gctaattttg aagatggtag gttgcatatg acctttgaac
     3601 aattcttcaa attggcacaa atagctagag aagaaggtct tgaaattcat tctccgtttg
     3661 agagagctgg tgcgactaaa tctgctcgtt atatagcgaa atggattttg agaaataaaa
     3721 aacattaaca aatatagttg gtaaatcatt aggacctaaa tcagctgtta gattcggaga
     3781 agctttatcc tatattgaag gtcctcttcg cagaataaat gagacgatag atggcggttt
     3841 atatcaaata gagcaaatta ttgcatctgg attgaaagaa tcgggtttaa atgactggac
     3901 tgcgaaaact ttagcttcag ctattcgtgg gatattagat gtacttattt aggggttgaa
     3961 atcatatgaa tattaccaat ttgttttcta tcaagacagg atgtgatgaa actgataggc
     4021 aactgcaaaa actatttttt cagttggatt tacaattggg agaattgaca gatcaactaa
     4081 gaaaattaga ttctaatttt gttcctcgta gtcaatttgt agacacgttg gatttgaatg
     4141 atgtagaata taaagaaatt ttaaactatt ttatcttcca tcgtaatgat agtgaagaaa
     4201 gtttggtaga atggttatat gattggattt ccacaaatcg ttatgaactt cctaaagagt
     4261 tttcgattcg tatggctcat aaataccatg aaagtgttac tgaagttttc ggagatgaat
     4321 aactaaaaaa cagtcattag tgactgtttt ttatagaaaa agaggtttta tatgttaagt
     4381 tcaaaagata taatcaaggc tcacaaggtc ttgaacggtg tggttgtgaa tactccactg
     4441 gattacgatc attatttatc ggagaagtat ggtgctaaga tttatttgaa aaaagaaaat
     4501 gcccagcgtg ttcgctcctt taaaattcgt ggtgcctatt atgccatttc ccagctcagc
     4561 aaggaagaac gtgaacgtgg ggtagtctgc gcttctgcgg gaaatcatgc gcagggagta
     4621 gcctatactt gtaatgaaat gaaaattcct gctactatct ttatgcccat tactacgcca
     4681 caacaaaaga ttggtcaggt tcgctttttt ggtggggatt ttgtaactat taaactagtt
     4741 ggagatacct ttgatgcctc agccaaagca gctcaagaat ttacagtctc tgaaaatcgt
     4801 acctttattg atccttttga tgatgctcat gttcaagcag gtcaaggaac agttgcttat
     4861 gagattttag aagaagctcg aaaagaatcg attgattttg atgctgtctt ggttcctgtt
     4921 ggtggtggcg gtctcattgc cggggtttct acctatatca aggaaacaag tccagagatt
     4981 gaggttatcg gagtagaggc gaatggagcg cgttccatga aagctgcctt tgaggctgga
     5041 ggtccagtaa aactcaagga aattgataaa tttgcggatg ggattgctgt gcaaaaggta
     5101 ggtcagttga cctatgaagc aactcgtcaa catattaaaa ctttggtagg tgtcgatgag
     5161 ggattgattt ctgaaacctt gattgacctt tactctaagc aagggatagt cgcagaacct
     5221 gctggagcgg ctagtatcgc ctctttagag gttttagctg aatatattaa ggggaaaacc
     5281 atttgttgta tcatttctgg aggaaataat gatatcaacc gtatgccaga aatggaagag
     5341 cgtgccttga tttatgatgg tatcaaacat tactttgtgg tcaatttccc acaacgtcca
     5401 ggagctttgc gtgagtttgt aaatgatatc ctggggccaa atgatgatat cacacgtttt
     5461 gagtatatca aacgagctag caagggaaca ggcccagtat taattgggat cgctttagca
     5521 gataagcatg attatgcagg tttgattcgt agaatggaag gttttgatcc agcttatatt
     5581 aacttaaatg gtaatgaaac gctttataat atgcttgtct gaggactaat aaaaaaatat
     5641 catattattt gtacaactga tgtatatgtg ctataatgaa gatgaagaaa ggggacgatt
     5701 cctatggttt tagaaaactt atcgtgcaat tcttacttct gttcattata gcacttatga
     5761 tgtttggctc agatttccat ccttcatttt gatttcctat ctattgacaa gcatagtcac
     5821 actgtcttta atactcttcg aaaatctctt caaaccacgt tagctctatc tgcaacctca
     5881 aaacagtgtt ttgagcaact tgcggctagc ttcctagt
//
LOCUS       DAVGJJ010000099         5291 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000099 DAVGJJ010000000
VERSION     DAVGJJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.099"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            97..168
                     /locus_tag="VVE35_001828"
     tRNA            97..168
                     /locus_tag="VVE35_001828"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Glu,seq:ttc)
     gene            421..1966
                     /locus_tag="VVE35_001829"
     rRNA            421..1966
                     /locus_tag="VVE35_001829"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2018..2090
                     /locus_tag="VVE35_001830"
     tRNA            2018..2090
                     /locus_tag="VVE35_001830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2051..2053,aa:Ala,seq:tgc)
     gene            2215..5116
                     /locus_tag="VVE35_001831"
     rRNA            2215..5116
                     /locus_tag="VVE35_001831"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5193..>5291
                     /gene="rrf"
                     /locus_tag="VVE35_001832"
     rRNA            5193..>5291
                     /gene="rrf"
                     /locus_tag="VVE35_001832"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1523 a         1062 c         1534 g         1172 t
ORIGIN      
        1 tagtaagtca tgcaaaaaaa atgaaaaaaa ttagaaaaag tagttgacaa agtttgaaaa
       61 gactgtataa tagtaagagt tgaaaataac aactcaggtc cgttggtcaa ggggttaaga
      121 caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatg gtatgttgcg
      181 tcaggaccac ttgatgaaaa aaagtttaaa aaaacttaaa aatcttcaaa aaagtgttga
      241 caagcgaaag cagttgtgat atactaatat agttgtcgct tgagagaagc aagtgacaaa
      301 gacctttgaa aactgaacaa gacgaaccaa tgtgcagggc gctacaacat aagttgtagt
      361 actgaacaat gaaaaaaaca ataaatctgt cagtgacaga aatgagtaag aactcaaact
      421 tttaatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      481 gtagaacgct gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg
      541 taggtaacct gcctggtagc gggggataac tattggaaac gatagctaat accgcataag
      601 agtagatgtt gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg
      661 cgttgtatta gctagttggt ggggtaacgg ctcaccaagg cgacgataca tagccgacct
      721 gagagggtga tcggccacac tgggactgag acacggccca gactcctacg ggaggcagca
      781 gtagggaatc ttcggcaatg gacggaagtc tgaccgagca acgccgcgtg agtgaagaag
      841 gttttcggat cgtaaagctc tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac
      901 actgtgacgg tatcttacca gaaagggacg gctaactacg tgccagcagc cgcggtaata
      961 cgtaggtccc gagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggttagata
     1021 agtctgaagt taaaggctgt ggcttaacca tagtaggctt tggaaactgt ttaacttgag
     1081 tgcaagaggg gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa
     1141 caccggtggc gaaagcggct ctctggcttg taactgacgc tgaggctcga aagcgtgggg
     1201 agcaaacagg attagatacc ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag
     1261 accctttccg gggtttagtg ccgtagctaa cgcattaagc actccgcctg gggagtacga
     1321 ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt
     1381 ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atccctctga ccgctctaga
     1441 gatagagctt tccttcggga cagaggtgac aggtggtgca tggttgtcgt cagctcgtgt
     1501 cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc tattgttagt tgccatcatt
     1561 tagttgggca ctctagcgag actgccggta ataaaccgga ggaaggtggg gatgacgtca
     1621 aatcatcatg ccccttatga cctgggctac acacgtgcta caatggctgg tacaacgagt
     1681 cgcaagccgg tgacggcaag ctaatctctt aaagccagtc tcagttcgga ttgtaggctg
     1741 caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat
     1801 acgttcccgg gccttgtaca caccgcccgt cacaccacga gagtttgtaa cacccgaagt
     1861 cggtgaggta accgtaagga gccagccgcc taaggtggga tagatgattg gggtgaagtc
     1921 gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa
     1981 ctgcgcattg gtcttgttta gtcttgagag gtcttgtggg gccttagctc agctgggaga
     2041 gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca ttggtgagag
     2101 atcaccaagt aatgcacatt gaaaattgaa tatctatatc aaatagtaac aagaaaataa
     2161 accgaaaacg ctgtagtatt aataagagtt tatgactgaa aggtcagaaa ataaggttaa
     2221 gttaataagg gcgcacggtg gatgccttgg cactaggagc cgacgaagga cgtgacaaac
     2281 gacgatatgc cttgggtagc tgtaagtaag cgatgatcca gggatttccg aatgggggaa
     2341 cccaacaggt aatacctgtt acccacatct gttaaggatg tgaggaggaa gacgcagtga
     2401 actgaaacat ctaagtagct gcaggaagag aaagcaaaag cgattgcctt agtagcggcg
     2461 agcgaaacgg caggagggca aaccgaagag tttactcttc ggggttgtag gactgcaatg
     2521 tggactcaaa gattatagaa gaatgatttg ggaagatcag ccaaagagag taatagcctc
     2581 gtatttaaaa tagtctttgt acttagcagt atcctgagta cggcgggaca cgtgaaatcc
     2641 cgtcggaatc tgggaggacc atctcccaac cctaaatact ccctagtgac cgatagtgaa
     2701 ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa
     2761 ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg
     2821 aaccggcgag ttacgttatg atgcgaggtt aagttgaaga gacggagccg tagggaaacc
     2881 gagtctgaat agggcgcctt agtatcatga cgtagacccg aaaccatgtg acctacccat
     2941 gagcaggttg aaggtgcggt aagacgcact ggaggaccga accagggcac gttgaaaagt
     3001 gcttggatga cttgtgggta gcggagaaat tccaaacgaa cttggagata gctggttctc
     3061 tccgaaatag ctttagggct agcgtcgaca ttagagattc ttggaggtag agcactgttt
     3121 gggtgagggg tccatcccgg attaccaatc tcagataaac tccgaatgcc aatgaattat
     3181 ggtcggcagt cagactgcga gtgctaagat ccgtagtcga aagggaaaca gcccagacca
     3241 ccagctaagg tcccaaaata attgttaagt ggaaaaggat gtggggttgc acagacaact
     3301 aggatgttag cttagaagca gctattcatt caaagagtgc gtaatagctc actagtcgag
     3361 tgaccctgcg ccgaaaatgt accggggcta aaacaattta ccgaagctgt ggataccttt
     3421 ataggtatgg taggagagcg ttctatgtgt gatgaaggta taccgtgagg agtgctggaa
     3481 cgcatagaag tgagaatgcc ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg
     3541 taagactaag gtttccaggg gaaggctcgt ccgccctggg ttagtcggga cctaaggaga
     3601 gaccgaaagg tgtatccgat ggacaacagg ttgatattcc tgtactagag tatgtagtga
     3661 tggagggacg cagtaggcta actaaagcag acgattggaa gagtctgtct aagcagtgag
     3721 gtgtgaattg agtcaaatgc ttaattctat aacattgagc tgtgatgggg agcgaagttt
     3781 agtagcgaag ttagtgacgt cacactgcca agaaaagctt ctagcgttta aacatactct
     3841 acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga gcgagagaac
     3901 tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt gctgacttaa
     3961 agtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct ctctgctaaa
     4021 tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct
     4081 tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac tataacggtc
     4141 ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt
     4201 gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat gcaggttacc
     4261 cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga gtgtctgtac
     4321 cacatgtaca ggataggtag gagtctaaga gatcgggacg ccagtttcga aggagacgct
     4381 gttgggatac tacccttgtg ttatggccac tctaacccag ataggtgatc cctatcggag
     4441 acagtgtctg acgggcagtt tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc
     4501 caaaggttcc ctcagaatgg ttggaaatca ttcgcagagt gtaaaggtat aagggagctt
     4561 gactgcgaga gctacaactc gagcagggac gaaagtcggg cttagtgatc cggtggttcc
     4621 gtatggaagg gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc
     4681 cccaagagtt cacatcgacg gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg
     4741 ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta aagcggcacg cgagctgggt
     4801 tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga
     4861 tctgctccta gtacgagagg accagagtgg acttaccgct ggtgtaccag ttgtcttgcc
     4921 aaaggcatcg ctgggtagct atgtagggaa gggataaacg ctgaaagcat ctaagtgtga
     4981 aacccacctc aagatgagat ttcccatgat tatatatcag taagagccct gagagatgat
     5041 caggtagata ggttagaagt ggaagtgtgg cgacacatgt agcggactaa tactaatagc
     5101 tcgaggactt atccaaagta actgagaata tgaaagcgaa cggttttctt aaattgaata
     5161 gatattcaat tttgagtagg tattactcag agttaagtga cgatagccta ggagatacac
     5221 ctgtacccat gccgaacaca gaagttaagc cctagaacgc cggaagtagt tgggggttgc
     5281 cccctgtgag a
//
LOCUS       DAVGJJ010000100         5237 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000100 DAVGJJ010000000
VERSION     DAVGJJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.100"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            67..1056
                     /gene="manX"
                     /locus_tag="VVE35_001833"
     CDS             67..1056
                     /gene="manX"
                     /locus_tag="VVE35_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU3061967.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1084..1887
                     /locus_tag="VVE35_001834"
     CDS             1084..1887
                     /locus_tag="VVE35_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU3061968.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1911..2822
                     /locus_tag="VVE35_001835"
     CDS             1911..2822
                     /locus_tag="VVE35_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU3061969.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTARPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2878..4212)
                     /gene="pepC"
                     /locus_tag="VVE35_001836"
     CDS             complement(2878..4212)
                     /gene="pepC"
                     /locus_tag="VVE35_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU3061970.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4430..5152)
                     /locus_tag="VVE35_001837"
     CDS             complement(4430..5152)
                     /locus_tag="VVE35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU3061971.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         1427 a         1155 c         1071 g         1584 t
ORIGIN      
        1 ttatacttaa acagtcttaa agttttctta aatgaaaact aaaacaaaaa aggaggaatg
       61 acaataatga gtatcggaat cattattgcg agccacggcg aatttgctgc gggtattcat
      121 cagtcaggat ctatgatctt tggtgaacaa gaaaaggttc aagttgtgac ctttatgcca
      181 aatgaaggtc ctgatgatct atacgctaag tttaataacg ctgttgctgc atttgacgca
      241 gaagatgagg ttctagtttt ggctgacctt tggagtggtt ctccatttaa ccaagctagt
      301 cgcgtgatgg gagaaaatcc tgagcgtaag tttgccatca tcacaggact taacttaccg
      361 atgttgattc aagcctacac agagcgcctc atggacgctg ctgcaggtgt agaaaaagtc
      421 gctgctaata tcattaaaga agccaaagat ggcatcaaag ctcttccaga agagctaaat
      481 ccagttgaag aagttgcaag cgctgcagct gctccagttg cccaaactgc tatcccagaa
      541 ggaactgtta tcggagacgg taaattgaaa atcaatcttg cccgtcttga cacacgtcta
      601 cttcacggtc aggttgcaac tgcttggact ccagattcaa aagcaaatcg tatcatcgtt
      661 gcttcagata acgtggctaa agacgacctt cgtaaagaat tgattaaaca agcagctcca
      721 ggtaatgtca aggctaacgt ggttccaatt caaaaactga ttgagatttc aaaagaccca
      781 cgttttggag aaacacatgc ccttatcttg tttgaaacac ctcaagatgc ccttcgtgcc
      841 atcgaaggcg gcgtgccaat caagactctt aacgttggtt ctatggctca ctcaacaggt
      901 aaaacattgg tcaataccgt tttgtctatg gacaaagaag atgttgctac atttgaaaaa
      961 atgcgtgact tgggtgttga atttgatgtc cgtaaagtac caaatgattc taaaaaagat
     1021 ttgtttgact tgattaacaa agccaatgtc aaataagcca ttatttatga aaggatttta
     1081 aacatgtcta ttatttctat ggttttagta gtcgttgtag ccttctttgc aggtcttgaa
     1141 ggcatcctcg accagttcca atttcaccaa ccacttgtag cctgtaccct tattgggctt
     1201 gtaacaggtc acttggaagc agggattatc ctcggtggat cgcttcaaat gattgccctt
     1261 ggttggtcaa atatcggtgc tgctatcgct cctgatgctg cacttgcttc tgtcgctgct
     1321 gccattatca tggttcttgg tggtgacttt accaaaactg gtatcggtgt tgcccaagcg
     1381 gttgctatcc ctcttgccgt agctggactt ttcttgacaa tgattgttcg tacaatttca
     1441 gttggtttgg ttcatactgc agatgctgcc gctaaaaaag gtgacttcgg cgctgtggag
     1501 cgtgcgcatt tcattgcgct actcttccaa ggacttcgta tcgcgcttcc tgcagctctt
     1561 ctccttatgg taccaactga aactgtacaa agtatcctta gtgccatgcc agactggctc
     1621 aaagatggta tggctatcgg tggtggtatg gtcgttgccg ttggttacgc catggttatc
     1681 aacatgatgg caactcgtga agtatggcca ttcttcgctc ttggtttcgt tctcgctgct
     1741 gtgtcagata ttactctaat cggattcggt gctatcggtg ttgctatcgc tcttatctac
     1801 cttcaccttt ctaaaactgg tggaaatggt ggcggaggag ccgcaacttc taacgaccca
     1861 atcggcgata tcctagaaga ctactaagat aagaaaggac tgaaaacatc atgactgaaa
     1921 aacttcaatt aactaaatca gatcgtaaaa aagtttggtg gcgttcaacc ttcttacaag
     1981 ggtcttggaa ctttgaacgg atgcaaaact tgggctgggc ttatacactc attccagcta
     2041 tcaaaaaact ctatactaaa aaagaagatc aaatcgctgc tcttgaacgt caccttgagt
     2101 tcttcaacac tcatccatac gtagctgctc cagtcatggg ggttactctt gcgcttgaag
     2161 aagaacgtgc taacggtgtg gaaatcgatg acgctgctat ccaaggggtt aaaatcggta
     2221 tgatgggacc tcttgctggt atcggtgacc cagtattctg gtttacagca cgcccaatcc
     2281 ttggatctct cggtgcttca cttgccctta ctggcaatat cttggggcca ctcctcttct
     2341 ttgttgcatg gaacttgatt cgtatgtcat tcttgtggta tgttcaagag attggataca
     2401 aggctggatc agaaatcact aaagatatgt ctggtggtat ccttcaagat atcactaaag
     2461 gagcttctat ccttgggatg ttcattcttg ctgtccttgt tcaacgctgg gtaaatatta
     2521 aatttgcttt cgatgtttct aaagttcaac tagatgaaaa ggcttatatc cattgggata
     2581 aattgccaga agggtctaaa ggtatccaag aagcattcgc acaagtagga caaggattgt
     2641 ctcaaactcc tgaaaaagtt actactttcc aacaaaactt ggatatgttg attcctggat
     2701 tatcaggact actccttact ttactttgca tgtacttact taagaaaaaa gtatctccaa
     2761 tcactattat ccttgccctc ttcgcagtgg gtattgtggc acatgttctt cacatcatgt
     2821 aatcaagcaa ctaaaaagga accaggttct aaaatctgat tccttttttc tatgctttta
     2881 ttcagccaag gctcccattg gatcccatgg tgcaagtacg attggttctg ctccataggc
     2941 agcttgttct tctgctgtca gcaattcctt acgaacaacg atttggtatg tgtattcgtc
     3001 catccaagcg tctgaggcaa caaagtaacc atctgtaccg accttgtctc cccatgagtt
     3061 ttcaaccttc cacttggttg atttaccatt ttcgtccaag tcaacacctg tcaagaccat
     3121 ggcgtgggtc atcaagcttt cgctatagtc caaacgtcca gccttgtctt gagtaagttt
     3181 aatgtccatg cttgattcaa agtcataaac atctgtcgca aggatgccag ccttacggtt
     3241 gctgagctgg ccgacatcag aaccaaacca aacagtctca cctgcttgca tttgggcaat
     3301 cgccaattct ttcaagcgct ccattggaac gttgatgtag cgaactgcac ggctaccaac
     3361 cacattcccc aacatctcaa ctgtgtaaga ttttccgtaa ggtttatcag cagttggagc
     3421 attgataaca gaaacgtagt cttctaaagg aagattgaca tatttcttgt aaaactcttg
     3481 tggtgtgatt cctttttcac ttttatagtt gttatcttta tcgcgataag caaagtcaaa
     3541 cttgcgtggt ggaagtccta atgacatagc aagaaagtta aagatttctt gcaagaggtc
     3601 ttctttctta gcttgaacag tcgcttgatc tgcaccagaa acaagcaaat cacgcaagat
     3661 ttgagcatct tgacgaagca atttattaag gatcgcattt agctcacgac tgctgctaga
     3721 tgaaacagac tcaggataaa ctgacttagg cacgacaccg tatttttcaa agagggaaac
     3781 gaccatatcc cattgaccgc catcttgttg aggtgtttgg agtaagaagc taaccttgcg
     3841 gctagtcaat tcttggtctg aagtcgcaat gacttgctcc aagaaccagt ttgatttctc
     3901 atacttatcc cagaagaaag tgtgggcttg tgacaactca aagttctcca atttgtattg
     3961 cgagatgagt ttgtggcgga aggtgttgag agccgcaaac atccagcaac gaccagacgc
     4021 tttctggtta gtgaccttgt ccttggttaa atccaatgag aaaacaggtg tgttgtctac
     4081 atggctttgg cgacattcca gagctgcaaa aattccgttg tggctggcag cattttcaat
     4141 cgcttggtat tttacatttg cttcatagtt ggcaaatagt ttatcagtaa atgattcttg
     4201 aatcgcgttc atagattcct ccttttattc tacagtctat tataactctt ttcacaaagg
     4261 aagcaactaa aaaacataaa aggcgaggac attgtccctc acctttttcg aatttatact
     4321 cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc cgtactcaag tacagcctgc
     4381 ggctagtttc ctagtttgct ctttgatttt cattgagtat taaactaaat taaataatat
     4441 tagcgcggag aatttctaat tcttccttgg tcaagcgacg ccattcccct cgttctaggt
     4501 tctcatctaa tactaaagtt cccatagtca atcgttgcaa gtccaccact tccttgccac
     4561 agtagcccac catacgcttg atctgatgaa acttcccttc tgcaatggtc acacggattt
     4621 ggctttgatt cttttctgta tctatggata caagctccag tatagcgggt tgacaggtaa
     4681 agtctttgag aggaataccc tcagcaaatg tctccacatc ttcttgggtc atgattccct
     4741 tgacttgtgc cagataagtc ttgtccacat gacgcttggg cgaaagaaga acatgagcca
     4801 gctgaccatc attggtcaag agcaaaagac catgcgtgtc aatatccaag cgtcctactg
     4861 ggaaaacttc cttactccgc gccaagtcat ccaacaagtc cagaacggtt ctgtgcttgg
     4921 gatcctcagt cgctgagata actcctttgg gcttgttcat catgtagtag acaaactctt
     4981 catactccaa cacttgccca tcaaagcgaa tctcatctat tttttcatca atctgcaatt
     5041 tagctgattt ttctttttta ccatttacag tcacgcgccc agccttgagc aagtttttga
     5101 cctcagtccg acttcccacc gcacaggcaa ctaaaaattt atctaatctc atagaactat
     5161 tatatcatgg aaattattgt tgtacataac taacatttta ttaggtgaac tattatcaat
     5221 tttatggtat agtataa
//
LOCUS       DAVGJJ010000101         5201 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000101 DAVGJJ010000000
VERSION     DAVGJJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.101"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            11..619
                     /locus_tag="VVE35_001838"
     CDS             11..619
                     /locus_tag="VVE35_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU3061972.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            650..853
                     /locus_tag="VVE35_001839"
     CDS             650..853
                     /locus_tag="VVE35_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU3061973.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(963..1241)
                     /locus_tag="VVE35_001840"
                     /pseudo
     CDS             complement(963..1241)
                     /locus_tag="VVE35_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1439..1726
                     /locus_tag="VVE35_001841"
     CDS             1439..1726
                     /locus_tag="VVE35_001841"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU3061974.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(2004..2774)
                     /locus_tag="VVE35_001842"
     CDS             complement(2004..2774)
                     /locus_tag="VVE35_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU3061975.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(2789..3637)
                     /locus_tag="VVE35_001843"
     CDS             complement(2789..3637)
                     /locus_tag="VVE35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU3061976.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFTQFVKETGYKTLAERQEWSFVFILFVPEAERERYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(3634..5109)
                     /locus_tag="VVE35_001844"
     CDS             complement(3634..5109)
                     /locus_tag="VVE35_001844"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU3061977.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKNKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALGGKLKKDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
BASE COUNT         1804 a         1033 c          827 g         1537 t
ORIGIN      
        1 ggagtttcat atgtcaaacg aaaaaaacac aaacactaac gtagaaaaga aagatgctac
       61 tgttgtagct cacgaaatca aaggggaact tacttacgaa gataaagtta tccaaaaaat
      121 cattggtctt tcactagaaa acgtttcagg tcttttggga atcgatggtg gtttcttctc
      181 aaatcttaaa gaaaaaatcg ttaacagcga tgacgtaaca agtggtgtta acgtagaagt
      241 tggtaaaaca caagttgcag ttgacttaaa cgttattgtt gagtaccaaa aaaatgttcc
      301 agctttatat tcagaaatca gagaaatcgt atcttcagaa gttgctaaaa tgactgactt
      361 ggaaattgtt gaaatcaacg taaacgttgt cgacatcaaa actaaagaac agcatgaagc
      421 agactcagta agccttcaag atcgcgtatc tgacgttgct gaatcaacag gagaattcac
      481 ttcagaacaa ttcgaaaaag ctaaatctgg tcttggatct ggtttctcaa ctgttcaaga
      541 aaaagttagc gaaggtgtag aagctgttaa aggtgcagca aatggtgtag tatctcacga
      601 aaacactcgt gtaaactaag ataaaataaa tataacagga gaaattatca tgtcagtaga
      661 agaaaaatta aatcaagcta aaggttctat taaagaaggt gttgggaaag ccatcggtga
      721 tgaaaaaatg gaaaaagaag gagcggctga aaaagttgtt tctaaagtta aagaagttgc
      781 cgaagacgct aaggacgctg tagaaggtgc tgtagaaggt gttaaaaaca tgttgagtgg
      841 cgacgataaa taaggttaaa agttacttta tctttttagt aacattagtc aaaagagtct
      901 gagtcaagat gattctcaga aaacaaaaag ctagagattc ccaattgcgg aactctagct
      961 ttttaatttt gcctctttct cttattatat ttcagcaggt tgttggccat gagtacgaat
     1021 cccatgtcaa ttctcacttg acgcttacct ctcagatgac atctcttata acccaaacaa
     1081 acctttatct gcccaaagac agatttcata tcaatcttac gtttagcgaa aatttgtcta
     1141 cccttggaag ataaaagtgc ctgatattct ttagttttta aacactggta acgttcattc
     1201 atatacagtc ccttttgagg ggctgattca ggttcataat cgcagtcaac attgatttca
     1261 aggctgtttg ctttctatct cccctttttc atgatatatt ttcgtacagg tgcttcaata
     1321 gtttctatag taaaatgaac caaaaatagt acacgttgcg gtataatgtt cttgtggcat
     1381 attcattaga ttttcgtaaa aagttctctc ctatttgtga gcgaacaggt atagtcaatt
     1441 gattgtcagt ttgaagacca agagtcgtaa ggcaaaggat atggcagaat ccatccaagg
     1501 gtggctggca caatttctag tgaacctctt taaatctatt acgtttgact gtggaaaaga
     1561 gttttcaaaa tggaaagata tttccaatca tcacgatagt gagagctttt ttgcgaatct
     1621 aggatgttca agacagcggc gtctcaacga acattcaaat agacttttaa ggtgtcatga
     1681 cttgccaaag caaacagatt tcaacgaagt cagtcaagaa ttttaggctg gacaaaaact
     1741 caatttcagg agaaaatgaa ataaatattc ccacaataaa acgcatagta tcaaagtttt
     1801 tccaacacct gatattatgc attttttaat tttaaagact ttttcccagc ctcaagaaat
     1861 cacatcccac ctaaatcctt tggataccaa atacctcatc aggtattttc tgagttggta
     1921 agactgtcta gcttaatttg acaaatggag ggcgttaaat ccagttttta caggaagtct
     1981 atttaaatat tttcacactg accttacaaa atatgaaata aattatataa agtcattccc
     2041 atgacaaaga ataacatccc tttaaaacag tatcttaccc aattttcagg taagactttt
     2101 gatacaaccg tccccaatac ccctccgcat atagaagcaa gaaaaataac tggaacaggt
     2161 gctaaatgat actgatttaa acctgtaacc acaacaattg tagccaaatg agacagctgg
     2221 gaaaagaaaa taattgcaag agaatacatc gttgcttctt ttatcgaaat agaaaaaaac
     2281 accatcaata aagatacatt gagcggacca ccaccaatcc ctaaaaaact agaaattaag
     2341 cccaatagaa gaccacaaat catatagaat cctatcttat caaattgata agttttttta
     2401 aaatctgtta aaacaaatga tacaagtagc atcacaaata gtaaaatcat ttgaacaatg
     2461 gatactagcc gtactgataa ctgagataac aaaacttgaa aaataagatg acctagaaat
     2521 cctcctaaaa cagaagaaaa ggataccgta aaaatcaatc tccattttat ctctttactt
     2581 tgtgaaaatc ttttggttgt tgaaataatc gccattataa aaacagagaa actagaataa
     2641 aaagcaatat cactgaccga atggtatcca aaactatcca taagtggctt aattataatt
     2701 ccaccaccca tgcctgatat agaacccaat ccactagcta ttataatgat tagaaaataa
     2761 ataatctcaa ccacttttta agtccatcct attctttcaa acatctaaaa cctagatggc
     2821 tactagtgga agtagaaata catccatttc tagcagctac tcggtatcga ttacaataag
     2881 aacaatgaca taaaaaggaa cctcctctga tagcatactc ctcatcttgt atcccatatt
     2941 ttggtaactc atagtcatct tcattaaaac tagctaataa ggtatatctc ggattgcgac
     3001 accattccca aacattccca atcatttgat atagaccaaa atcattaggt aaaaattcat
     3061 agacaggagc cgttcctata aatccatcta aggcagtatt ttcataagga aatcttcctt
     3121 gccaagtatt ggcatgataa tatccacctt ctaaaagagt atctccccaa ggatattcag
     3181 attgtcttcc tcctctagca gcatattccc actgtgcttc agtcggtaaa gacattccgc
     3241 tccaattaca gaaagccaat gcatcttcta aagcaacatg aactactgga tggtcttcta
     3301 aacccactag attactattc tcaccgtagg gatgtttcca gcaagcgttc ggaacttgta
     3361 accaccacgg agccccagca ggatgaggat atctctccct ttctgcctct ggaacgaaca
     3421 atataaaaac aaaagaccac tcttgtcgct cagctagtgt tttatagcct gtttctttca
     3481 caaattgagt aaattcttga ttcgtaactg gagtatccgc aatggagaaa tcctttacac
     3541 taacagcgac ttgaggacct tcaaaatcat ctaaaaagcc ctcttcagaa tttgttccca
     3601 tcataaatgt cccacccttg atttttttca ttttcatgat tgactatccc ccgttttcgt
     3661 tagagttggc gttattttag aaagttcaac aggaactaac tcttcatcca caacaaaacc
     3721 ttcttctcta tatctaagaa agtctactaa ttttttcttc atctgacgaa caataggttg
     3781 gtatttttca cttggataca aatcattcat ttcatgcgga tcttttttca tatcaaataa
     3841 ctgataatga tttaaaacag gaaaccagat aaatttccac tggtctgtca agatatattg
     3901 actagaatct tttcctaaag catgttcacc atgaaattct gttcgccacc cctcgtactg
     3961 tccaaataat aaattcttaa cactccgtcc atctagttca tctgtagtag ttcctcctgc
     4021 caaatccacg agtgaaggaa aaatatcttg aattttcact aattgcttga ttgttcccct
     4081 attaccagct atcaatccag ccggatcgta aataaacgaa ggaatgtgga tacttccctg
     4141 ataaggatat ccctttctaa acagataatg ttcaccaagc tgatctccat gatccgaaac
     4201 aaaccagata atcgtatctt tatcatgacg aaattctttt agggcagtca aaaagcgact
     4261 aatttgatgg tctatatgag taatcaatcc aaaataagca gcaaccattc ttctttgatc
     4321 atctttcttt aatttccctc ctaaagcata aatagacgga atttgctttt ctaatacttc
     4381 ccaattcccg atatgcaaat cgagaaattg aggcaagcgt tccatataca tatcaaaata
     4441 gtattttggg ggatttaatg gagcatgtgg tttttcaaaa gacattttga gaaaaaaagg
     4501 aaccgttgga tctcttcttt gtaaaaatga aatagactca ctcacaaccc agtttgttgg
     4561 atgtaacttc tcatctttat cccaaggtct agcttcccaa gaattacaat ccattccgtc
     4621 atcaatcaaa tcaacatcat aaccaacttt accctttaaa aaggctagat agtcactagc
     4681 ataatcaaat tgacttccat aagccttatc atactttcta tcaacatgta gatacccatc
     4741 gtgaagtaag acatgatcaa acccaagtct ctgtctggaa ggaaaaacat gcatcttacc
     4801 aatacactct gtttgataac ccatatcttt aaagactttt ggaagagtat tagtaaaatt
     4861 ccaaggcacc tcatcttgat aaccaacacg tccactttta tcttggtcta agccagtcaa
     4921 taaagctgcc ctagcaggaa cgcaagaagg aactggagaa taggcatttt caaaattata
     4981 acctacacta gccatcatat ctaatgttgg agtgctaact agcttattct tactattcaa
     5041 ggataaggcg tctgctctca tttgatctac aacaatcaaa ataatatttg gctgttttgt
     5101 ctgaaccata aaatctcctt tctaatatgg caaaagaggc acaagaagat atctaccttt
     5161 actgcacccc tttctatatc aatctctcta tataaaacaa t
//
LOCUS       DAVGJJ010000102         5087 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000102 DAVGJJ010000000
VERSION     DAVGJJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.102"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            101..478
                     /gene="mscL"
                     /locus_tag="VVE35_001845"
     CDS             101..478
                     /gene="mscL"
                     /locus_tag="VVE35_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU3061978.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(520..1614)
                     /gene="hemH"
                     /locus_tag="VVE35_001846"
     CDS             complement(520..1614)
                     /gene="hemH"
                     /locus_tag="VVE35_001846"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU3061979.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPGHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            1769..2992
                     /gene="pepT"
                     /locus_tag="VVE35_001847"
     CDS             1769..2992
                     /gene="pepT"
                     /locus_tag="VVE35_001847"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU3061980.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTTIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(3028..3657)
                     /locus_tag="VVE35_001848"
     CDS             complement(3028..3657)
                     /locus_tag="VVE35_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061981.1"
                     /translation="MNNANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            3622..3843
                     /locus_tag="VVE35_001849"
     CDS             3622..3843
                     /locus_tag="VVE35_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061982.1"
                     /translation="MDLFQELICIVQKTFYFFLSICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(4114..4461)
                     /locus_tag="VVE35_001850"
                     /pseudo
     CDS             complement(4114..4461)
                     /locus_tag="VVE35_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145489.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(4562..5087)
                     /locus_tag="VVE35_001851"
                     /pseudo
     CDS             complement(4562..5087)
                     /locus_tag="VVE35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         1469 a          999 c          930 g         1689 t
ORIGIN      
        1 ctatattttt agaaaataat gctccattta tgagcctttt aattgccttc tataatgatt
       61 taggatacaa tgaaacacga aacattgaaa ggaaaacagt atgttaaaaa atctaaaatc
      121 gttcttgctt cgaggaaatg ttattgacct tgctgtcggt gttgtcattg cctctgcttt
      181 tggtgctatc gttacttcac ttgtaaacga cattatcact cctcttattt taaatcccgc
      241 tttgaaagct gctaaagtcg aacgtatcgc tcaactttct tggcatggag tcggctatgg
      301 taacttttta agtgctatta tcaattttat ctttgtgggt accgccctct tctttattat
      361 caagggcatt gaaaaagcac agaaactgac tggcataaag aaagaaaaaa ctgccgaaaa
      421 aaaaccaacc gaattggaag tccttcaaga aataaaagct ctccttgaga aaaaataatc
      481 gattaaaagg atctagcgca aagctaaatc cttttttctt tactctttgg gtaacttgcc
      541 tgctttttca agcatttttt tgataagata aggcatctta acattctccc gccctttttt
      601 ctcaatcagt tttttcacaa attctggcat ttgtaaatct tcagattcag ctaaaatgtt
      661 tttcgtctcg tctgaaggaa ctaactcatc aaaggtctct tcttctggga gaaattcctt
      721 aaactcttga ttttcattga ctctaactgt attgaccaaa gcatcaatca aacgagaatc
      781 tgtatttggc attggtggac gatggtagtt taccccaaat tcctggcata agtcataaca
      841 ttctacatca ttgtcaaaca agacctcaat atgctcactg atgaaactaa taggtacaaa
      901 aatataatgg cctggatgtt ctgtctgttc tctgagatac tccaaaacat ctggtttaat
      961 ccatggaata ccaatatcac tctcactctg ccaagtgttg gtatattgtt ctgaactcaa
     1021 gcccagtttt tcagcgacta gcttgctatt ttcgaaaatt tggtctatat aaggatcacc
     1081 aaagtccaag gcaaaaatgg gcacactatg ggctgaaaag atgactttaa agctgtcttg
     1141 ctttacttct tcttttaaaa tcttagcaat ttcatctgcc caatagttta agagcgcttc
     1201 ttcctgatac cagtccttaa tgaccaaaaa ttgaatttgt ttgctttcta aaaatttttc
     1261 atagcccatg acagagtaga aagaataatg gggctccaaa atcaagcaaa tacactgttc
     1321 aattccgtct gcttccattt gaccgatcac atctggaata aagggactgg aaaacttatt
     1381 agcaaagtag acactgtatt cattccccag cctagcttct accaaggcaa cctcttgacg
     1441 ggtaattttt tgcagaggcg tgcctccaat tcgtacataa ttatcataga gagtctgaat
     1501 ctcgtggtct tggggtctca ccccacgacg aatatttgtg aaaaaatcag ctacaccttc
     1561 aaaggtaatc tcttctggtg aaccgaaggt catcattaaa attgcttttt tcatgactgc
     1621 ttccttgtga tgtttttatc aagtttattt tatcattaat tcatcgataa gtaaacgcta
     1681 acatagggac attctcaatc ttctgtcttt tgtttttctc ttctttctat aatacaatgg
     1741 aaaaaataaa ttcaaaagga gtttttttat gacttatcca aatctcttgg accgcttctt
     1801 aacctacgtt aaggtcaaca cgcgctctga tgaacactct actactactc caagtacaca
     1861 gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa atgaaacgtg ttggactgca
     1921 aaatgtttac tatctaccga atggttttgc gattggaacc ttgccagcca acgatccgtc
     1981 tttaacacgt aagattggtt ttatatcgca catggatact gctgatttta atgctgaagg
     2041 agtcaatcca caggtaattg aaaactacga tggtggtgtg attgaactag ggaattctgg
     2101 tttcaaactc gatccagctg acttcaagag tcttgaaaaa tatccaggac aaacgctcat
     2161 cacaacagat ggaacaagct tgctaggtgc tgatgacaag tcaggaattg ctgaaattat
     2221 gacaaccatt gaatatctaa ctgctcatcc tgaaattaag cactgtgaga ttcgtgttgg
     2281 ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa tttgatgcag aagattttga
     2341 tgtggatttt gcctacactg ttgatggtgg tccactaggt gaacttcagt acgagacttt
     2401 ctcagccgct ggtgctgaat tgcatttcca aggccgtaat gtccaccctg gtactgccaa
     2461 agggcagatg gtcaatgccc ttcagctagc aattgatttt cataatcaac ttccagaaaa
     2521 tgaccgacct gagttaactg aaggttacca aggtttttac catctaatgg atgtgacagg
     2581 tagtgttgag gaggtgcgtg caagctacat cattcgtgat tttgaaaaag atgcctttga
     2641 agcgcgtaaa gcatccatgc aatctatcgc tgataagatg aatgaagaac ttgggagcga
     2701 ccgtgtcact ctcaacttga cagaccagta ctacaatatg aaagaagtca ttgaaaaaga
     2761 tatgactcca attaccattg ctaaagccgt tatggaagat ctaggtatca cgcctattat
     2821 cgaaccaatc cggggtggaa cagacggctc taagatttcc tttatgggaa tcccaactcc
     2881 gaatatcttt gcaggtggcg aaaatatgca cggacgtttt gaatacgtta gccttcagac
     2941 tatggaacgt gcagttgata ccatcattgg catcgtagct tataaaggct aaaaagacga
     3001 ggtagctcag ctacttcgcc tttcttttta ttctactggt ttttcttgat ttccagtagt
     3061 tgtagaagat tctgttgttt cattttctga agttgattca gcaggtttag aatctcttgt
     3121 attgcttggt ttgttttcgt cgctagcagt ttcaatgtta gattctgcag ttgcgtttgg
     3181 ttggttctca gcactggtgt tatcactatt tgcttcagca tttcttgctg gacttgtttc
     3241 ttcacttgcg ctagcttttg actggatttg atgattcaaa actagaatag cttttgtcga
     3301 ttcaagtaaa gctgttttgt ctttactctt agcagaaagt tgatctaata atgcatccac
     3361 cttatcaaag tccgcatcag atccattatt actttctaaa taagagtgaa gcgacatgag
     3421 aatatcgtag agtttttgat agagtacaag tgtctgagaa tcttgctcag cattttcctt
     3481 ttcttgttga agggcgctag cgatacgagt caaaacatct tttacctgac tgtttacttc
     3541 atccaagtct gcatcagcct tgtttgtggc agcttttaga ttttctactt cttctgccaa
     3601 ggattgtctg attccttctt catggatttg ttccaagagt tgatttgcat tgttcaaaag
     3661 actttctact tcttcctttc tatctgtcgc agattattgg ttgctatcta ccatgtactc
     3721 ctaaaacagg agagttataa tccaagatta caaggcctta cagaaataag aaatccagat
     3781 aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc agcacggatt caatatactt
     3841 taattttaaa gtttaggtgt caagacctct ttttagtgtg cccaaaattt agagaagtaa
     3901 tcaatcaact aacttttatt tttttcaaac tttcagtaaa ctgacctaaa gctaactcaa
     3961 tctgtctttg tagatgcttc tgctatcagc tagaagttga tctacttttg ccaagactgc
     4021 cttctcatca aaagttccag gttgatagtt ggattgcagg gatggaatct tgtttttcaa
     4081 agccgcttca tatcccttag tttgaacctt gatgtagtga ttgtggtcgc catgaggaat
     4141 cacaaaacct tctgaatctt cacttataat tcgattggca tcaaaaacca tgaccatctt
     4201 cttcctcatg gtggacatgt agtgacgatt acttaataca gaactagaag aacttcctac
     4261 ctcttccgtg ttagagtgtg atgggggatt gttaagagat gacttaggaa tatagtgata
     4321 gtgttcccca tgtcttacta tataagcatc acctatatct ctgacaatat cattagggtt
     4381 aaagacatat gtggctgcta attcacctgc cgacaagtca ctctcaggaa tgaaatgata
     4441 gtgaccacca tgtggtacta tagtagattg aaatagaata tgagcaaatt gataagggga
     4501 ttttaaagta atttctaaca atgatttaga aactatgatg tgctattcta aattcaactc
     4561 actatatata accatcatcg gtagtataac gtccctgtaa ttttgctaca gatacttctg
     4621 cactagctcc tttatcgtct ttaccatgtt cttgtttttg gcgattgatt tcatcttttg
     4681 ttcgtacatt ttctgcatga gcttgatctt taaggtaaac ataatacttt ccatctacct
     4741 taataatata tcctccctta acctaactga caatatcttg atctttcggc tgatagttgg
     4801 gggctttcat taatggctct tcactaaaga gcgcatcaaa aggaacttta ccattatagc
     4861 agtgataatg atcgccatga gaagttacat aaccttgacc tgtaatctta ataacaattt
     4921 gttttgcttg aattccttct ttttgactaa cctagtctgg agtcaaattt tcagtcttct
     4981 tagtgtcttt attactgttt acatatgaaa cacgattttt atctgtattg gcctgttagc
     5041 tatgttggtt cagagcataa acacacagac ttaaggaaag gataaca
//
LOCUS       DAVGJJ010000103         4878 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000103 DAVGJJ010000000
VERSION     DAVGJJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.103"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            63..827
                     /locus_tag="VVE35_001852"
     CDS             63..827
                     /locus_tag="VVE35_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061983.1"
                     /translation="MLTRQQSLTRLELEDNELAIKSRIDSISQQIMKSKRIVQEKEEA
                     IKQLQEQFNQYHERLTELKQLSDIDEYVNQNVLSELKKLEIQETSEKSNLDKLIGDIT
                     KEIRMLKKELNQRAKTIEGDYEALKNELSILIGSTGITDKKFRNFDKLKGSGTNLNKD
                     LLVIFLVYMNLIDSKSITSLPFAIDSFVKNETDEKVLKKMFDAINSKFLSLKSQTFFS
                     IIRDNLKYVDSKVNQVNIESPLLKREYYNDLSKEII"
     gene            complement(921..2186)
                     /locus_tag="VVE35_001853"
     CDS             complement(921..2186)
                     /locus_tag="VVE35_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU3061984.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKKS"
     gene            complement(2186..2767)
                     /locus_tag="VVE35_001854"
     CDS             complement(2186..2767)
                     /locus_tag="VVE35_001854"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU3061985.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(3167..3262)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(3645..4031)
                     /locus_tag="VVE35_001855"
     CDS             complement(3645..4031)
                     /locus_tag="VVE35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU3061986.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPIYRPLTKRKF
                     RVSDYYIYPHKFAVLDPNGYFLRFSE"
     gene            complement(4043..4870)
                     /gene="xth"
                     /locus_tag="VVE35_001856"
     CDS             complement(4043..4870)
                     /gene="xth"
                     /locus_tag="VVE35_001856"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU3061987.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
BASE COUNT         1579 a          900 c         1039 g         1360 t
ORIGIN      
        1 tatcaagtaa gattaaggag agattttctg aaattagaca tgaatgtgtt tattgccatt
       61 ctatcttaac tagacaacaa tccctaacac gcttagaact ggaagacaat gaacttgcaa
      121 tcaagagtcg aatagatagt atttctcagc aaattatgaa atctaaaaga atagttcaag
      181 aaaaagaaga agctataaaa cagctacaag aacagtttaa tcaatatcat gaacgtttaa
      241 ctgaactgaa acaattatct gatattgatg agtatgtgaa tcaaaatgtt ttatctgaac
      301 taaaaaagtt agagattcaa gaaacttcgg aaaaatcaaa tcttgacaaa ttgattggtg
      361 acattacaaa agaaataagg atgctcaaaa aagagcttaa tcagagagcg aaaacaattg
      421 aaggagatta tgaagcactt aaaaatgaac tttcaattct aattggttca acaggtatca
      481 cagataaaaa attccgtaat tttgataagt taaagggcag tggaacaaat ttaaataaag
      541 atttattggt aatttttctc gtttatatga acttgattga tagtaaatct atcacaagtc
      601 tgccatttgc tattgattca ttcgtaaaaa atgaaactga tgaaaaggtt ttgaaaaaaa
      661 tgttcgacgc aataaattca aaatttctaa gtttaaaaag tcaaacgttc ttttcgatta
      721 ttagagataa tttgaaatat gttgattcga aagttaatca ggttaacata gaaagcccat
      781 tattaaagcg tgaatattat aacgatctgt ctaaagaaat tatataagtt agaatcaaaa
      841 gtatacttac taaaacaagt aggctacaaa aaagcggtct tcaacgaaaa gttgacaacc
      901 gctttttaat tatgcgactt tcatgatttc tttttatgat ttagtaccgc attgaggaca
      961 atggcgagta ggctggctac gacgattccg tttgagaaga acatttggaa ggctgtcggc
     1021 atgctgacaa agagattact gttgttgaga ccgacacctg cagcgattga aacagctgcg
     1081 ataaggaagt tgtgttcatt gttagcaaag tcaactcggg cgaggatttg catcccttga
     1141 atagatacaa aaccaaacat caccagcatg gcaccaccga ggacggagct tggaatgatt
     1201 tgggcaaggg caccaaactt aggaagcagt ccaaggagaa ccaggaaacc agctgcgtag
     1261 tagattggca ggcgtttttt gatgcctgac aatttaacca aaccaacgtt ttgtgaaaat
     1321 ccggtgtaag ggaaggtgtt aaagattcct ccgagaagta cggccaaacc ttctgcgcgg
     1381 tatccgttgc gaaggcgcgt gctgtcgatt ggatcttttg tgatatcaga caaggctaga
     1441 taaacaccag ttgactcaac catagacacc gttgcgataa tacacatcat gacaatagat
     1501 gagatttcaa aggttggcat cccaaagtag agtggagttg ggacatggac aagtggagct
     1561 accgcaacag gagagaagtc caccaagccc atagtagcag caatggcagt tccaacaacc
     1621 agaccaatca aaatagagat agacttgata aatcctttgg taaagatatt gatcaagagg
     1681 ataatcagaa cagtaatagc tgaaagcaag agactttgac cagttggctc tggaacgtta
     1741 tttcccatat ttccaatagc gacagggatc aaggttaaac caatcgtggt aataacagat
     1801 cctgttacga tagatgggaa aagattggct acttttgaga agatgcctga aacaagaacc
     1861 acgtaaatcc cagatgcgat aagggcacca aacatagcgc cactaccatg gctttgccca
     1921 atcataatca agggagcgac cgactggaat gcaactccaa gaacgactgg gagtccaatc
     1981 ccaaagtatt tgttgagttg gagttggagg aaggttgcca ccccacacat gaagatatct
     2041 gtagaaatca ggtaggtcaa ctgctcagct gaatagccaa gggctgtcgc aatcatgatg
     2101 ggaaccagga tagatcctga gtacatggct agtaagtgct gcaagcccag aacggctgct
     2161 tgcgagtgtt tttcttgagt ttgcattaga gatctgcctc cttaaatacg acctgaccat
     2221 tttcaaaacg atccaaacga gcaagtgata ggacagggta gcctgccttt tcaagcaaat
     2281 cacggccatc ttggaaggat ttctcaatca cgataccgat agcttggact gtggcaccgg
     2341 cctgttcgat gatttgaatc aagcctttag cagcttggcc attagcaagg aaatcgtcga
     2401 taatcaaaac cttgtcctct ggtgagagga attttccagc gatagaaacg gtgctggtca
     2461 cctgtttggt aaaggagtag acttgagcag ttaagatgcc ttcgttcatg gtgatgttct
     2521 tagctttttt ggcgaaaatc atgggaacgt ttaaggcttc agctgtaaaa acggctgggg
     2581 caatacccga cgcttcaatg gttacgacct tggtaatgcc agtagcagca aatttttccg
     2641 caaaaacctt accaatctct cgcatcaagc taaagtcaac ttggtgggtt aaaaaggaat
     2701 ctaccttgag gatgttatca cccaagatat gcccatcctt gaggatgcgc tcttctaata
     2761 atttcataag acctcctaaa gtctaaaagc taatttattt gttgtttaaa tgtttctata
     2821 gtgatccctt ttgctaatac tatatatttg ataaaactat tacgagcgaa gcgagtctta
     2881 tcaaatattt cccgttgtag tggtatcata gacaatgatc ttgttattgt ctatgacggg
     2941 atttttgaga gtaaaatagt tcggggaact attttagcct aagcctagaa atgaaagagc
     3001 taggggctca aaaattaggg atgaaattcc ctggattcct gaaattattc acaggataat
     3061 ttcacctccc gtccgcacta attaagggaa atattaaaaa tattcaaaaa ggacctactt
     3121 aatctctaag taagtccccc aaataggcat ggcaaaaacg gccatatctc actgctgact
     3181 tacttattgt taggtgttcc ggcaccttgt agaaacgtcg tgccaattca cgacataaac
     3241 aagtaaaacg atattcaatt ttaaataggc ttgagccaat gtttttattt tacactaaat
     3301 gactttagaa atcaactatt ttgttagtat tttggtttaa aaaacgaaca aacagaagag
     3361 ggggtgagca aaaactccat tgtaagctaa catttatact aaatgaaaat caaagagcaa
     3421 actaggaagc tatccacaac ctcaaaacac tgttttgagg ttgtggatag aattgacaga
     3481 gtcagtatca tatacctacg gtaaggcgac gttgacgtgg cttgaagaga ttttcgaaga
     3541 gtattagaag atttttccat cataaaaggc atactatcaa gtttttaaac acctgacaat
     3601 atgccttttt ctaactttaa agacttttcc caatttttat tattctactc gctaaatctt
     3661 aaaaaatagc catttggatc caaaactgca aatttatgag gatagatata gtaatcactg
     3721 acacgaaact ttcttttggt caagggacga taaataggat agtttgcctc catcactttt
     3781 tgatagagtt ttggaacatc ctttatgcca aaggagagat tgactccacg accaaatgga
     3841 taggtcagtt cagctagttg atcctttgtt ccctcctcta acattagttg acactcttca
     3901 agagaaagaa agagaaagtt ttcttctgga cgttggtatt caatcctaaa acccagtaaa
     3961 ccacagtaga aggaccggga ctgttcgata ttcgatacaa gcaactcggg aatgaccgca
     4021 ttgtagtcca tatagaaaat ccttacaagt caatttccaa gacaatcggt gtatggtctt
     4081 ggcgagcacc tgagtcaatc atatcagatt tagtgacctt gtcagcgata cggttacttg
     4141 tgagccagta gtcgattctc cagcctgtat tgttgatttt agaagttttg ctgcgttgtg
     4201 cccaccaagt gtagcgttca ggaacatcgc catgaacatg gcggaaggtg tctgtaaatc
     4261 cagttgccaa aaggttggta aatccagcac gttcctcgtc agtaaatcca ggtgaacggc
     4321 ggttgctagc aggatttgca aggtcgattt cattgtgggc tacgttgtag tcaccggtcg
     4381 caaggactgg tttttctttg tctagttcag ccaaatactc agcatattta acatcccaga
     4441 cttggcgttc ttccaagcgt ttgagaccgt caccagcgtt tggagtgtaa acttgggtta
     4501 cgaaaaatgc atcaaattct agagtgatga tacggccttc caagtccatg gtagaagggg
     4561 caccgatttc tgggaagctg atcgtaggcg taagttcttt cttataaagg aacatggttc
     4621 cagcatagcc tttacgggca ggctcttggg aagagcgcca cgtgttttcg tagcctggga
     4681 agagttcttc taaaatttcc acgtgtttct ttgtaggtcc tttggcagaa agcttggttt
     4741 cttggatagc aataatatca gcattttcag cgaccaaggt ttgtaggact tcttgggaca
     4801 atttggcacg agctgagtca ctagttaggg cagcgtttag ggaatcaata ttccatgaga
     4861 taagtttcat aaagttac
//
LOCUS       DAVGJJ010000104         4827 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000104 DAVGJJ010000000
VERSION     DAVGJJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.104"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            108..806
                     /locus_tag="VVE35_001857"
     CDS             108..806
                     /locus_tag="VVE35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU3061988.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDSIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     misc_feature    871..958
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            994..1308
                     /gene="rplU"
                     /locus_tag="VVE35_001858"
     CDS             994..1308
                     /gene="rplU"
                     /locus_tag="VVE35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU3061989.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1324..1668
                     /locus_tag="VVE35_001859"
     CDS             1324..1668
                     /locus_tag="VVE35_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU3061990.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            1685..1978
                     /gene="rpmA"
                     /locus_tag="VVE35_001860"
     CDS             1685..1978
                     /gene="rpmA"
                     /locus_tag="VVE35_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU3061991.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2289..2494)
                     /locus_tag="VVE35_001861"
                     /pseudo
     CDS             complement(2289..2494)
                     /locus_tag="VVE35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2931..3848
                     /locus_tag="VVE35_001862"
     CDS             2931..3848
                     /locus_tag="VVE35_001862"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU3061992.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3941..4789)
                     /locus_tag="VVE35_001863"
     CDS             complement(3941..4789)
                     /locus_tag="VVE35_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU3061993.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1528 a          823 c          984 g         1492 t
ORIGIN      
        1 tggataaata gttgtctatt attattttta tgctaatagt aaaatctaaa tggtagccta
       61 cgatttttta gaaagaaaat ggtacaatat ttctaagaga aaatacaatg ggaggtaaaa
      121 tgaggttatt acctataaga aaaatatcac gtcagtctaa aaggttagca ctttttttga
      181 cgttttgtgc tggatatgtg gatgcttaca cttttattgt tcgcgggaat acccttgtag
      241 ctggacaaac tggaaatgtt gtctttcttt cagtagaatt aattaaaaat aatgtttcgg
      301 atgttaggga caaggttctc accttgctag cgtttatgat gggagtcttt ttattaacga
      361 tttataagga aaaattgaga attgtgaaaa aacctattct gtccttgatt cccttggcaa
      421 tcttatcaat cattattgct tttgtgccgc aaactgtgga tagtatctat ctagtgccgc
      481 ccttggcctt ctgtatggga ctggtgacaa ctgcttttgg agaagtgtcg ggtattgcct
      541 ataataacgc ttttatgaca gggaatatca aacggaccat gctggctttt ggagattatt
      601 tccgaaccaa gcacactcct tttttgcgtg aaggattcat atttgttagc ctgcttagta
      661 gttttgtcct tggcgttgtc ttttcagcct atttgacgat tttctatcat gaaaagacca
      721 ttcttggtgt tcctattatg atgagcgttt tttacctcag catgcttttt gcctcttggc
      781 agaaaaaagt aaaagaaaaa gcttgatttt aggtgttatt acttgtaaga aaataggaga
      841 tatgctatac ttgaagagtt gactatgcac gatcctgtgc aaccgcacga acatcgttgc
      901 ttgattattc aagtttaagt ggttcgtccc acgatgctag gcgagtcttc acaaaaaatc
      961 ggggaaaccc ataaaaatca taggaggtgc ataatgagca catacgcaat tatcaaaact
     1021 ggcggaaaac aagttaaagt tgaagttggt caagcagttt acgttgaaaa attgaacgtt
     1081 gaagctggtc aagaagttac ttttaacgaa gttgttcttg ttggtggtga aaacactgtt
     1141 gtcggaactc cacttgttgc tggagctact gtagttggaa ctgttgaaaa acaaggaaaa
     1201 caaaagaaag tggttactta caagtacaaa cctaaaaaag gtagccaccg taaacaaggt
     1261 caccgtcaac catatacaaa agttgtcatc aacgcaatca acgcttaatt ttaaggagaa
     1321 cacatgatac aggcagtctt tgagagagcc gaagatggcg agctgaggag tgcggaaatt
     1381 actggacacg ccgagagtgg cgaatacggc ttagatgtcg tgtgtgcatc ggtttctacg
     1441 cttgccatta actttatcaa ttctattgag aaatttgcag gctatgaacc aatcctagaa
     1501 ttaaacgaag atgaaggtgg ctatctgatg gttgaaatac caaaagatct tccttcacac
     1561 cagagagaaa tgacccagtt attctttgaa tcatttttct taggtatggc aaacttatcg
     1621 gagaactctt ctgagttcgt ccaaaccaga gttatcacag aaaactaaca cggaggaaaa
     1681 cattatgtta aaaatgactc ttaacaactt gcaacttttc gcccacaaaa aaggtggagg
     1741 ttctacatca aacggacgtg attcacaagc gaaacgtctt ggagctaaag cagctgacgg
     1801 acaaactgta acaggtggat caatccttta ccgtcaacgt ggtacacaca tctatccagg
     1861 tgtaaacgtt ggtcgtggtg gagacgatac tttgttcgct aaagttgaag gcgtagtacg
     1921 ctttgaacgt aaaggacgcg ataaaaaaca agtttctgtt tacccaatcg ctaaataaaa
     1981 aggtccattg aaccttttat cccgaacctt gaaatgtaaa ggtgaggaag ctagaaacag
     2041 cttaaaataa ggctttccga gttttcggag agcctgtttt tttgttttga tatccatact
     2101 ttttaatatg tatagtggat tgatactctt cgaaaatcaa attcaaacca cgtcagtttc
     2161 accttgccgt actcaagtac agcctgcggc taacttccta gtttgcactt taattttcat
     2221 tgagtatgaa tctggaatag aacactgtta aaaattgata tgagtttcct actcgatttc
     2281 ttcagatttt atttcaattg actataagaa ctgctatctg gtttcaaata ttggcttgtc
     2341 gtcccttcac ttgtctgaat ggtttgagcc gttccttctg ataagggaaa agatattctt
     2401 tagctgaaac ggtctgagaa tccaagctaa cagatggaga gaaaaatagc ccagtctgaa
     2461 gcacactaga tgggagttgt tggaaagcag tcatctagtt tttctttgct gttttggctt
     2521 ttttctttgt catcagggaa agtgacttgc ttcaaatcag ggagtttagg aaattcttca
     2581 aacatcttgc ccaagttgtc tcattttgta aattttacag ccataggtgt acctcgattc
     2641 taaattctac cttttcggca ccaattagta atttaaagtt ctcaggagtt agttgcattg
     2701 ctttttcata cataacatag tatgacactt tatggttacc gaacaagtct aatcactcct
     2761 tgcaaaattt tgaagatgtc ctacaagttt ttgagaagaa tattgcaaag ttcatagtta
     2821 agcaacgata gaataaagtg ctttttgaaa acaggtacac ttcatgtgaa aaaaacaaca
     2881 tttcagattt tctgttccag aaatagctat cgctatgata taattatttt atgattatta
     2941 ctattcctat aaaaaaccaa aaagatattg gcacaccatc tgattcagtc gttgttctcg
     3001 gctattttga tggcatacat aaggggcatc aagaattatt tcgtgttgcc aacaaggctg
     3061 cgagaaagga tttattacct atcgtcgtta tgacctttaa tgaatctcca aagatcgctt
     3121 tagagcctta tcatccagat ttgtttttgc atattttgaa ccctgctgaa cgtgaaagaa
     3181 aattaaagcg cgaaggtgta gaagaattat atctccttga ttttagtagt caattcgcta
     3241 gtctcactgc acaagaattc tttgcaactt atatcaaggc tatgaatgcc aaaattattg
     3301 ttgcaggttt tgattataca tttggttctg acaaaaaaac agcagaagat ttaaaggatt
     3361 actttgatgg agaagttatc attgttccac ctgtagaaga tgagaaagga aagattagtt
     3421 caactcgtat ccgtcaagct attttagatg gaaatgtgaa agaagcagga aaacttttgg
     3481 gggcaccgct tccatcaaga ggtatggtgg ttcatggtaa tgctcgtggt cgtacaattg
     3541 gttatccgac agcgaattta gtgcttttag accgtactta tatgccagca gatggcgttt
     3601 atgtcgttga tgttgagatt caaagacaga agtatcgtgc tatggctagt gtcgggaaaa
     3661 atgtgacctt tgatggagaa gaagcacgtt ttgaagtcaa tatttttgat tttaatcaag
     3721 atatttatgg ggaaaccgtc atggtttatt ggcttgatcg cattcgtgat atgaccaaat
     3781 ttgactcagt tgaccaatta gtggatcagt taaaggctga tgaagaagta actcggaatt
     3841 ggtcttaaga gcttgagtaa ataaaacaaa aaagaggttg tctgtaaccc aaaagataga
     3901 tgatttagtc taacttttga ggtcacgaca ttacctcttt ttattctttt tcaaaggtga
     3961 agccttctcc taggatttca tgggcttctg taatagttat aaaggcttga ggatcgattc
     4021 gatgaatcat ttccttcgtt ttcacaattt catttcttcc gacaatacag tagatgattt
     4081 tcaaattttc tttactatag tagccttgac cagaaataaa agtaacacct cttccgaggt
     4141 catcattaat cgccttagca agttggtcag gacgttttgt gataatcata aagcctttgc
     4201 cggcatatcc tccttcacca atcaaatcaa taacacgaga aacaataaaa tcaaacaaaa
     4261 gcgtgtagga aaccaatctc aaatccttga agattaggag aatcaacatg agaatacaaa
     4321 aatctaagat aaagagcagt tttcctatgg atatatgagt gtatttgttg agaatacgag
     4381 ctagaatatc agttccgcca gttgtacctc cagcattaaa aataattcca aggccaattc
     4441 ccaataggat tcccgctata agggctgtga ttagtaaatc accttgaaga tcaatatgaa
     4501 ggggaatatg ctcaaaaaaa gctaaccagg cggacaaagc taaggttcct agtaaactag
     4561 aatagaggga tttggctcca aagatcttcc aagctaggat gaaaagggga atattaatca
     4621 gcaggttcat gagggaaaca gggattttaa aaagataaaa ggtgatgagg gtaatgcctg
     4681 tcgcccctcc ttcaaagaga tgatggggaa ctacaaaata agtcagtcca aaagcataaa
     4741 tagcagcacc tagtaaaatg gtaaaaatgg ggtaaatttt tttaatcata ggacacctct
     4801 tttcttataa gttgattata tcaaatt
//
LOCUS       DAVGJJ010000105         4610 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000105 DAVGJJ010000000
VERSION     DAVGJJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.105"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(485..1036)
                     /locus_tag="VVE35_001864"
     CDS             complement(485..1036)
                     /locus_tag="VVE35_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3061994.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1386..2210)
                     /gene="nadE"
                     /locus_tag="VVE35_001865"
     CDS             complement(1386..2210)
                     /gene="nadE"
                     /locus_tag="VVE35_001865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU3061995.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTVAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2207..3667)
                     /locus_tag="VVE35_001866"
     CDS             complement(2207..3667)
                     /locus_tag="VVE35_001866"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU3061996.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGSLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3783..4271)
                     /locus_tag="VVE35_001867"
     CDS             complement(3783..4271)
                     /locus_tag="VVE35_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3061997.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4261..4470)
                     /locus_tag="VVE35_001868"
     CDS             complement(4261..4470)
                     /locus_tag="VVE35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3061998.1"
                     /translation="MVKNLKLKLARVELDLTQGQLADAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1340 a         1061 c          842 g         1367 t
ORIGIN      
        1 aaaggattgc agacatctta tctaaataca tgctaatata tttagataca aacattccaa
       61 cttgataatt ttcactcatc tttcatcatt ccttatacaa ctatgcagta taaatagaat
      121 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
      181 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
      241 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
      301 tagctccctc ttagttacaa gcttaaagcg attttttaac taacactaaa ccagattaaa
      361 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
      421 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
      481 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
      541 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
      601 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
      661 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
      721 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acaatgctga
      781 tatctcctat cacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
      841 gattagtata ggaagcaacc caattgcaaa tcgagtttcg agtgatttcg acatcaggat
      901 ggggatccca ggtaacatag gtcagattct cagcggatga agcccaattt tgaaacatgg
      961 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
     1021 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
     1081 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
     1141 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
     1201 gatgaaatcg aactcttcga gttactgaat ctgcctcatt ttcttatttc taaactcggg
     1261 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
     1321 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtcccccgga
     1381 cctttttact cccaaaagtc atcaaatacg gtgatgggta agtggcgttt gtgttggcct
     1441 ttgtgccacc agttttcaat ggtcgcttga gcttctgggc tgattgtttt gccttctagg
     1501 tagtcgtcaa tctctgcgta ggtgactcca agtgcgactt cgtcagctag gcctggttta
     1561 tcttcttcta ggtctgccgt tgggattttt tcataaaggg ctggctctgc gccaagtttc
     1621 tgcaagagct gttttccttg gcgtttattg aggcggtaaa gagggagaat atccgcaccg
     1681 ccgtcaccaa acttggtaaa gaaacctgtg atattttccg cggcgtggtc tgttccaatg
     1741 accgctccgc tatgggaacc agcaagggca tactgagcaa tcatacggca acgtgccttg
     1801 atattcccct tgttgaagtc tgaaacagga ctacctgtcg cttcaactgc aactgtcatg
     1861 gcatcagctg attccttgat attcacaacc aagctgacat ctggctgaat gaaggctagg
     1921 gctttttgag catctgcttc atcagcttgc actccgtatg gcaggcggac agcgataaat
     1981 ttgtagctgt cgtctcccgt ttcagctcgc agttcttcca tagctaattg cgccaaacgc
     2041 cctgccaagg ttgagtcttg tcccccagaa atccctagta caaaggtttt taggaaggga
     2101 tgttttttca gatatctttt taagaaatca atagaacgac ggatttcttc ctgggcatca
     2161 atcactggtt tgacacccag ctcttggata atcgtttctt gcaaactcat tcttcttctc
     2221 cttcaccaag ggcttccttg cgcatcttat caatcaagtc catcttatct tgccatacat
     2281 cacgcgccaa atccactgga tagtgctgcg gattgagcac acgcttatac tcatcccaca
     2341 acttgtcaaa ttccttacgg gcataatcct gaatgtcagt caaactaggc aagttgtaaa
     2401 ctaatattcc ttctttgaag atatccacca agagaggaac ggcatcaaaa ttacgaaccg
     2461 tcttcttgat gtatgtatag gtcggatgga acatcttgat ttctgtcatg tcgctaatat
     2521 ccacaccatc ataagtgata tagtcgcctt ctgacttgcc tttttcacga ctggtaatgc
     2581 gccacacctg cttcttacct ggcgtagaga ctttttccgc attattagac agcttaatcg
     2641 tattgcgcat ctgaccagtt tcatcttcga ttgcaacaat cttgtaaacc gccccaagag
     2701 ctggctggtc ataggctgta atcagcttgg tacccacgcc ccagacatca atcttggcct
     2761 tttgcatctt gaggttgagg atagtatttt cgtccaaatc attagaagca taaatcttag
     2821 cctctgtaaa tccagcctcg tccagttgct gacggacttt cttagaaatg taggcaatat
     2881 ccccagagtc aatccgcaca cccataaagt taatctgatc acccagctca cgcgccacct
     2941 gaatggcagc tggtacaccg atgcgaaggg tgtcataggt atccacaaga aagacacaat
     3001 ttttgtgggt cgcagcgtaa gccttgaaag cttcatagtc attgccataa acctgtacca
     3061 aggcatgggc atgggttccc aaaacaggaa tgtcaaagag cttacccgca cgcacgttgc
     3121 tggttccatt ggcgccacca atcacagctg cgcgtgttcc ccagatggcc gcatccattt
     3181 cttgagcccg acgtgtccca aactccatca agggttcatc ttcgataacc gaacgaatac
     3241 gagctgcctt cgtcgccacc aaggtctggt agttgacgat gttcaaaaga gccgtttcga
     3301 ccaactgaca ttgggctaga gatccttcca cctgcacaat cggttcatta gcaaaaacca
     3361 aatccccttc ttgggcagaa cgaacggtca actccaactt gaaattgcga aggtaatcca
     3421 agaacgcccc atgataacca agcgactcca aataggctat atcactatct gaaaaacgca
     3481 agtcttcaag atagttcaca attctttcca aacctgcaaa aaccgcatag ccgttcttaa
     3541 aaggctgttg gcggaaatac acctcaaaga ccgccttctt attgtgaatc ccttggtcaa
     3601 agtaaacctg catcatgttg atctggtaca agtccgtgtg caatgtcaaa ctatcatctg
     3661 gatacatact tttcctactt ccttagctag aaacccatga aaattttcaa gaactttcat
     3721 gtattccaat aaattagtac tattatatca cattttagct ggattgagaa aagagtaaca
     3781 agctattctc cactctccaa ttcatccata tcttgttcaa attttttctg agcccattcg
     3841 ccatagctct taagaccaag attgccaata aagacccacg gaaggtaaat gacataagta
     3901 atgacccaag cagacaggta tttaaaattc aaaggattgt gctgataaat ttctatgttg
     3961 aattgataat tctgcaacat caaaagagcc gtaatagcca aggttaggaa aaaacaaccc
     4021 aaaatcgtaa aatgaaaacg actatagtag gtcactccca gataacgggc gcgattgaaa
     4081 aagtaaaatg tccctatgat gataacgatt agcagcatat tagaattaaa aaggcttggt
     4141 gctaatactg aaatgatata agataggagc gacaaagcaa tgcagatata gaaactttca
     4201 gagcccgctt tattgaacag ttgttcttct ctttcgtcta gtaattgata ataataaaat
     4261 ctatttttca tcttcttcct cccaaaatag ttggtctagg gttttcccta aacatctgca
     4321 aatggactgg cagagcgaga gactgggatt gtattttccc gcttctatca aaccaatagt
     4381 ctggcgcgtc accccgacag catctgctag ttgaccttga gttaaatcaa gctctacccg
     4441 agctaatttt aattttaaat ttttaaccac cttcgtcctc cttataattt taatattcat
     4501 ctactcttaa aaaattccaa actaacacaa gctgtcagct atttattata actccattaa
     4561 tttgcactcc agtattatct tcagccgaaa atcagtattt taggttgttt
//
LOCUS       DAVGJJ010000106         4350 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000106 DAVGJJ010000000
VERSION     DAVGJJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.106"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1252
                     /locus_tag="VVE35_001869"
     CDS             <1..1252
                     /locus_tag="VVE35_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3061999.1"
                     /translation="LQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMVIASF
                     PIPGWEKYLGDIGVADYLWKGVDSSFELLGLVASFGIAYFMARQYKVDGIPAGIVSLS
                     SFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMPDGVP
                     PAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVIGLL
                     ILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFMDNFV
                     FIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVLNIL
                     LLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQIVL
                     IVLDVLLYLPFVIAIEKRFKLLED"
     gene            1255..2151
                     /locus_tag="VVE35_001870"
     CDS             1255..2151
                     /locus_tag="VVE35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU3062000.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTREAFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHGKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            2166..2483
                     /locus_tag="VVE35_001871"
     CDS             2166..2483
                     /locus_tag="VVE35_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3062001.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            2500..2817
                     /locus_tag="VVE35_001872"
     CDS             2500..2817
                     /locus_tag="VVE35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3062002.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2827..4182
                     /locus_tag="VVE35_001873"
     CDS             2827..4182
                     /locus_tag="VVE35_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3062003.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
BASE COUNT         1262 a          618 c          982 g         1488 t
ORIGIN      
        1 attacaagaa aagttacttc cagtagcggc tagattgggt aataataaag cgttggtttc
       61 tattcgagac ggtattactc taactatacc tttattgttg ataggttcat tactaatggt
      121 tattgcaagt tttcctattc caggatggga aaaatatctg ggtgatattg gtgttgctga
      181 ctatttatgg aagggtgttg atagtagttt tgaattactt ggtcttgtag ctagttttgg
      241 tatcgcttac tttatggcta gacaatacaa agttgatggt attcctgctg gtattgtgtc
      301 attatcatct tttattacag ttacgccttt cattagagga gaggctggag caggaatgcc
      361 taccgctttc atggcatcaa aaggtttatt tgttgctatg attttaggat tgattaatgg
      421 ctatatctac caatggttta taaatcataa tatacagata aaaatgccgg atggtgttcc
      481 accagcagta tctaaaagct ttagtgccat tattcctggt gcagtgacta ttgttggttg
      541 gttgattgtt tatgcaactt tagataaatt aagtttacct aatcttcatg aaattgctca
      601 agtagctttg ggaggtccac ttggactttt aggaaataat gttattggtc ttcttatctt
      661 aattttcctt aatagtagtt tttggtttgt aggattacac ggaggaaatg ttgttaatgc
      721 agttatgaaa ccgttatggt tagctaatct agatgcaaat aaagtagctt atcaaactgg
      781 agaaactctt ccaaatattt ttactagcgt atttatggat aattttgtat tcataggtgg
      841 cggtggtgca accataggtt tagttcttgc gttaggatat ttagcacata agaagaaagc
      901 tagtaaacaa ctaaaaactt tggcacctat aactgttata cctggattat ttaatataaa
      961 tgaaccagca atgtttggcg ttcctattgt tttgaacatt ttgttactag ttccatttat
     1021 cttagctcca atgtttaact tactagtagc gtggggagca atggcatcag gtttggttcc
     1081 gctaacttat acagatccag gttggactat gccacctgtt ataagtggtt tacttgctac
     1141 aggaagtatt tctggttcat tattacaaat tgtattgatt gttttggatg tcttacttta
     1201 tttaccattt gtaatagcta ttgaaaaacg gtttaagtta ttggaggatt gagaatgaaa
     1261 cgtttaatta gtgcaaatcc atctgagata ttacagatga atgctgaaga attaaaacaa
     1321 agtattttag caagtgaagg tagagttgtt ctatctgaaa atgtagttac tcgtgaggca
     1381 tttgttgggg atataactaa ttctgaaatt gctagagctt ttggagctga tatgatttta
     1441 ttgaattgtg ttgatgtttt tgagcctaaa atttatgctt tggatagttc aggtgatgat
     1501 gttattcatc gcttacacca gcttgttgct tgtccaattg gtgtaaattt ggaaccgatt
     1561 gacccatctg caaagatgct agaggaaaca caggaaattg ttgcaggtcg tgttgctagt
     1621 gttgaaacat tgaagcgaat agaggagtta ggttttgact ttgtctgttt gactggaaat
     1681 cctggaacag gagttagcaa tcgagaaatc attaaggctg ttcaaactgc taaggaaaac
     1741 ttttctggtt tgattattgc aggtaagatg cacggggcag gagtgaatga gcctgtggca
     1801 gagctttctg tcgcagagca attgttggaa gcaggtgcgg atgtgatact tgttccagca
     1861 gttggaaccg ttccagcctt tcatgaccaa gagttgcgtg aagtcgttga tctcgttcat
     1921 ggtaagggtg gtcttgtatt gagtgctatt ggtactagcc aagaaacatc tgatacagat
     1981 actattaagg aaattgcact tagaaataaa atttgtggag ttgatattca acatataggt
     2041 gacgcaggat atgggggact ggcaacagtc gataatattt atgcattgag caaggcaatt
     2101 agaggagtga gacatacagt atctcgcttg gctaggtcag taaataggtg ataaaggagt
     2161 aagctatggc taaagtaaca attatgttag catgtgcagc aggtatgagt acaagtctgc
     2221 tagtgacaaa gatgcaaaag gcagcagaag ataaggggtt ggatgcagaa atttttgcag
     2281 ttccagctcc tgaagcagaa gaaattgtag caacaaagga agtaaatgtg ttgcttttag
     2341 gccctcaagt tcgctattta ctaggggact ttcaagaaaa actaaaagat agacagattc
     2401 ctgtggcggt tattccgatg acagattatg gaatgatgaa tggttctaaa gtcttagatt
     2461 tagctgaaag tttattagac taagattgag gagaatgcta tgaatgaaag taatttagaa
     2521 tctgcaatgg ggctaattat gtatggtggg gaagccaaaa gtaatgctat ggaggctatt
     2581 caggcagcaa aaaaagggga tttctctaaa gccaatcgaa gattagctga tgcgaatgct
     2641 gccttattac aggcgcataa ggctcaaaca gaaatgttga caagagaggc acagggggaa
     2701 gaaacatcaa ttagcctctt gatggtacac gcgcaagatc atttaatgac gagtctgact
     2761 tttgttgact tggcgaaaga agtggtagaa gtatacgaac gatttgaaaa aaattaggag
     2821 tttagtatga atacaatgtt ggataaaatg caagaaaaac tttctccaat tgcaatgaaa
     2881 gttgggaatc agaagttttt agttgcttta cgtgattcat ttgtggggac tatgcctgtt
     2941 attatgacag gttccattgc tttgttatta aatgcttttc tagtggattt accacaacaa
     3001 tttcacctag aaagtattac gaaaaccttt cagtggcttg ttgacatcaa taatttggta
     3061 ttcaagggaa gtataccaat tgtttcctta ttgttcatct attgcttggg agtgaatatc
     3121 gctaagattt ataaggttga tacagtttct gctggtttgg tatcgcttgc ttcgtttgtc
     3181 atttcgattg gaagtacagt tacaaagagt ttccctttgg caaatgtagg agatgtgaaa
     3241 ttagatcaaa tcttacaagg aattgataat ctagcatttg atggtaaaaa tctgatggta
     3301 actattggga atgtgattcc aggaaaccat attaatgcca gaggctactt tacagccatg
     3361 atgattggat ttttagcctc tattattttc tgtaaagtaa tgaaaaagaa ctgggtaatc
     3421 aagttaccag attcagttcc ccctgcgatt gctaagccgt tcacttctat cattccagga
     3481 tttatggcga tgtatatagt agccatcttg acttatgttt tccatttact tagcaatgac
     3541 ttattgattg attgggttta caaggtgtta caaacgccat tactaggttt gtctcaaagt
     3601 ttctttgcag ttattctgat gatcttttta aataagttat tctggttttt tggtctccat
     3661 ggaggaaatg tattggctcc catcatggaa gggctgtttg gagttgctat gttagcgaat
     3721 ctggatgctt tccaaaaagg tgaaccgatt ccttatatat ggacaagtgg gtcttttggg
     3781 gcgtttgtct ggtttggagg attaggattg gtacttgcta ttttaatttt ttcaagaaac
     3841 agtcattatc gaaaagttgc taaacttggt ctagcaccag ttctgtttaa tattggtgag
     3901 ccggtaaatt atggtttacc agttgtcttg aatcctttgc tatttatacc atttgtactt
     3961 agcccggttt tcatggcaac ggtagcttac tgggcaacaa gctggggtct tgtttcacct
     4021 gttacacaaa atgttacttg ggtaatgcct ccaattttat atggtttctt ctctacagca
     4081 tttgattggc gtgccatcat cttatcagtt gtttgtttga ttattagtgt cttgacttat
     4141 ttcccatttg tgaaaatggc agataaaact gaattgagtt aatggtctta cttgcgtttt
     4201 ttgtgataaa atagaaatag attacgtgta agattgagtt aggtgtagga tttcttatct
     4261 ccactaaaat tgttattaga caaaggttta tagtttattg aagttggaat agtacactgt
     4321 aacttctaaa acagtactag aaaatgattt
//
LOCUS       DAVGJJ010000107         4095 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000107 DAVGJJ010000000
VERSION     DAVGJJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.107"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            93..1502
                     /locus_tag="VVE35_001874"
     CDS             93..1502
                     /locus_tag="VVE35_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU3062004.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1520..2815
                     /locus_tag="VVE35_001875"
     CDS             1520..2815
                     /locus_tag="VVE35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU3062005.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2820..3128
                     /locus_tag="VVE35_001876"
     CDS             2820..3128
                     /locus_tag="VVE35_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU3062006.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3125..3433
                     /locus_tag="VVE35_001877"
     CDS             3125..3433
                     /locus_tag="VVE35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU3062007.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1380 a          678 c          775 g         1262 t
ORIGIN      
        1 caagatatgt atcaacaatt tttaaaaatg tctagacaaa ttttaaaatt atgctatact
       61 ttatgtatag aataatagaa aggaggttga gcatgaaaaa aaagttagta tttcctaatc
      121 tgttttggtg gggagctgct tctagcggac ctcagacaga aggtcaatat ggaaaagtac
      181 atgaaaatgt gatggactac tggttcaaaa cgcatccaga agattttttc gataatgtcg
      241 gacctcttgt agccagtaac ttttttcata cttacaccga agatttccac ttgatgaagg
      301 aaattggagt taattctttc cgcacttcca tccaatggag tcgactcatc aagaatttag
      361 agacaggtga gcctgatcca aaaggtattg ctttctacaa tgccatcatt gaagaagcta
      421 aaaagaacca gatggatctt gtgatgaatt tacatcattt tgatttacca gtggaacttc
      481 ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga gttattcgtg aagtttgcca
      541 agactgcttt cacatgcttt ggagataagg ttcattactg gacaactttc aatgagccaa
      601 tggtcattcc agaagcagga tacttatatg ctttccatta tccaaatcta aaaggaaagg
      661 gaaaagaggc cgtacaagtc atctataatc taaaccttgc tagtgcaaaa gtgattcaac
      721 tatatcgctc attaggactt gatggaaaga ttgggattat tttaaacttg acacctgctt
      781 atccaagaag taattctcca gaagacttag aagcaagtcg atttacagat gacttcttta
      841 acaaagtttt cttgaatcca gctgttaaag gaactttccc agaaagattg gtaaaacagc
      901 tagagagaga tggcgtgtta tggagtcata ccgaaaaaga gcttcaactg atgaaatcaa
      961 atacggttga ttttcttgga gtaaactact accatccaaa acgtgttcaa gcacaagcaa
     1021 atcctgagga atatcagacg ccctggatgc cagaccaata cttcaaagag tatgaatggc
     1081 cggagcgtcg catgaatcca tatcgtggtt gggaaatttt tccgaaagcc atttatgata
     1141 ttgctatgat tgtgaaggaa gaatatgata atattccatg gtttatcagt gaaaacggaa
     1201 tgggtgttga aaacgaagca cggtttatcg gtgaaaatgg agttatcgat gacgtgtatc
     1261 gtattgaatt ttatgaagaa catttaagat ggctacataa agccattgaa gagggaagtc
     1321 actgttttgg ataccacgct tggaccgcat ttgattgctg gtcttggaat aatgcatata
     1381 agaatcgtta cggatttatc tccgttgatt tagaaacgca aaagagaacc atcaagagct
     1441 caggaagatg gtatcgcaaa gtaagtgaca ataacggttt tgaagtagaa attgaggagt
     1501 aaagagagga gatattcaga tgaaaaactc aaaatttata gatcaatttg ccacctttgc
     1561 tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga gatgcattcg taacagtaat
     1621 gccattatat attttggcag gtttaatcgt tcttttgaac aacacggtat ttaagtggat
     1681 tttccaaggg gatacattaa caagattcca atattgggga ataacaattg caaacggtac
     1741 tttaagtatt tcaggtatga ttattgctgt aatggttggt tatttcttag ctaaaaacag
     1801 agatttcgaa aacccgttag cagcatcaat gctatcatta gtttctttaa ttgtgatgat
     1861 gccaaataca gtttctgtag ttcctgacgg agcaaaagat gcggtaaaca tttcaggtgt
     1921 tctttcattc aacaacacag gtacaggcgc aatgttcgcc ggggttatcg tagcgattat
     1981 tgcaacagaa ttattcattg aattatcaaa cgttaaagct ttacaaatga accttggtga
     2041 aaatattcca ccagctgtta gtagatcatt tagcgtatta cttccagtca tgaccgtcat
     2101 ctccttattt ggggttgttt cagcattatt attcaatata actggaatga acttaatctc
     2161 aatcattaca atctttattc aagaaccaat tcgtcatatt ggtacaagct taatcggggt
     2221 cattattatt tactctttag gaaatatgtt atggctattt ggtattcacc aagcagttat
     2281 ttacagtgcc atcctagaac cattactatt aattaacatt actgaaaaca tcactgcagc
     2341 aaataatgga caagccattc cacacatcat caacctatca caaatacaaa cattcgcttt
     2401 aatgggtggt agtggatcta cattatgttt attaatagca acattcttag tgagtcgcaa
     2461 tgctgtctct aaaaacgtgg ctaaattatc ttttggacct ggtatcttca atatcaatga
     2521 accagtatta ttcggttacc caatcgttta taacatttca ttagctattc catttatcac
     2581 agttccagtc cttggtattt taatcagcta cttagcaaca gttacaggat tcatgagtcc
     2641 tgcatttata caagttcctt ggactacacc agtattctta aatgcatggt tagcaacagc
     2701 aggggacgtg agagcagttc tagttcaatt catcatcttt gcacttggag ttcttctata
     2761 cattccattt atcaaagtta atgacaaagt tgttgaacaa gaaatggaag gttaagatta
     2821 tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac aagtatgctt gttaaaaaaa
     2881 tgcaacaaag tgcgactgaa cgtgggattg aaatttctat ccaagcaaaa tcaatgacag
     2941 aagcgaaaaa gaatatccat gaagcagatg tcattctaat tgggccacaa attcgttatg
     3001 agttacttgc tgtgaaagaa attgcaggaa atatccctgt agataccatt gatatgcggg
     3061 attacggaat gatgaatgga gcaaaagttc tggaacaagc acttgcatgg ataggagaaa
     3121 taagatgaca gaagaaatgg aatatatctg ttttcagctc attgccaata gtggagcagc
     3181 caaatcatcc tttattgaag ccatccaact agctaaggca ggaaatttaa aagaagcgaa
     3241 aattaaagtt gaagaagcgg aagactcctt agtagaagcg cacaagattc actctaatct
     3301 catacaaaaa gaagctactg gagaaaaaat aggattctcc ttgctattta tgcatgcaga
     3361 agaccaaatg gcttcaacag agattattca gttgctatct aaagaattca tcgatctcta
     3421 tcaaaataaa taggaacact gtcgttccca tctgtcttta ttaatgacgg ctacattgta
     3481 ggaacctcca aaagttggac aaaatgattg aaaggattgg atcctgcatt acacaagatc
     3541 caatcctttt cttttactct agtttttata atgtgttgaa tttagaatag tccactgcag
     3601 tttctaaaaa attgctggaa atagatgtta agctccaatt tatttgttta tatcttattt
     3661 cagtttacta tactagctta ttattctagt aaaaaattga atgagccact atttatttaa
     3721 tatgtatgat gagggaatga ctcaaaaact tcttgtcgat ataagaacat ctgagatagt
     3781 attttaaaac gaaaggacta taaaagctca tttccttcgc acttatttta tagagatcta
     3841 tcattatact ctttagaaat caaaaagcaa cacaccttgt catcgtttta tcgtaaacct
     3901 gttaatgagg ggctagtctt atctgcaatc tcaaatcagt gttttgagct gaatggcact
     3961 cgttttctag tttgctcttt gattttaatt gggccttagt aaatactact cttaaattaa
     4021 actatagtga attgaaataa gatgtgaaca actctatcag gaaagtaaaa ttaatttcta
     4081 taaatgtttt agcaa
//
LOCUS       DAVGJJ010000108         4058 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000108 DAVGJJ010000000
VERSION     DAVGJJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.108"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            275..1561
                     /locus_tag="VVE35_001878"
     CDS             275..1561
                     /locus_tag="VVE35_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU3062008.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1576..1887
                     /locus_tag="VVE35_001879"
     CDS             1576..1887
                     /locus_tag="VVE35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062009.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2133..2861
                     /locus_tag="VVE35_001880"
                     /pseudo
     CDS             2133..2861
                     /locus_tag="VVE35_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            3297..>4058
                     /locus_tag="VVE35_001881"
     CDS             3297..>4058
                     /locus_tag="VVE35_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062010.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLANLYGVDYNQERFGSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNCLFEIAFLSATDRDIFITKQEFWDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETL"
BASE COUNT         1320 a          640 c          851 g         1247 t
ORIGIN      
        1 tttccattat aggagcaata gagtgggagt agtcatctaa ggactaattt atgtattttt
       61 acgagtcagt atctttggat actggttttt acttttctag actttttgac tacttgttaa
      121 aactggggta attttcgact gtttaacagt tattatgcaa agtctaaaag attagaattg
      181 tcaaaacaat ccgtctaggc ttgattttat cctttattta ctataaaatg agaaggaaaa
      241 atgtcaaact tttatattgc aaataggaga aatcatgaca aaaacattaa aacgtcctga
      301 ggttttatca cctgcaggga ctttagagaa gctaaaggta gctgttcagt atggagcaga
      361 tgctgtcttt atcggtggtc aggcctatgg tcttcgtagc cgtgccggaa actttacttt
      421 cgaacagatg gaagaaggcg tgcagtttgc ggccaagtat ggtgccaagg tctatgtagc
      481 ggctaatatg gttatgcacg aaggaaatga agctggtgct ggtgagtggt tccgtaaact
      541 gcgtgatatc gggattgcag cagttatcgt atctgaccca gccttgatta tgattgcagc
      601 tactgaagca ccaggccttg aaatccacct ttctacccaa gccagtgcca ctaactatga
      661 aacccttgag ttctggaaag agctaggctt gactcgtgtc gttttagcgc gtgaggtttc
      721 aatggaagaa ttagctgaaa tccgcaaacg tacagatgtt gaaattgaag cctttgtcca
      781 tggagctatg tgtatttcat actctggacg ttgtactctt tcaaaccaca tgagtatgcg
      841 tgatgccaac cgtggtggat gttctcagtc atgccgttgg aaatacgacc tttacgatat
      901 gccatttggg aaagaacgta agagtttgca gggtgagatt ccagaagaat tttcaatgtc
      961 agccgttgac atgtctatga ttgaccacat tccagatatg attgaaaatg gtgtggacag
     1021 tctaaaaatc gaaggacgta tgaagtctat tcactacgta tcaacagtaa ccaactgcta
     1081 caaggcggct gtggatgcct atcttgaaag tcctgaaaag tttgaagcta tcaaacaaga
     1141 cttggtggac gagatgtgga aggttgccca acgtgaattg gcaacaggtt tctactacgg
     1201 tataccatct gaaaatgagc agttgtttgg tgctcgccgt aaaattcctg agtacaagtt
     1261 tgtcgctgaa gtggtttctt atgatgatgc ggtacaaaca gcaactattc gtcaacgaaa
     1321 cgtcattaac gaaggggacc aagttgagtt ttatggtcca ggtttccgtc attttgaaac
     1381 ctatattgaa gatttgcatg atgccaaagg caataaaatc gaccgcgctc caaatccaat
     1441 ggaactattg actattaaag tcccacaacc cgttcaatca ggagacatgg ttcgtgcatt
     1501 aaaagaagga ctcatcaatt tttataagga agatggaacc agcgtcacag ttcgagctta
     1561 agaaaggaaa aggaaatgat agaggcacag ggtttcttag tggataagca aacaagatgc
     1621 attcattacc atagcaagct ggatattatt gctttacaat gctatgattg taaaaagtat
     1681 tatgcttgtt atcggtgtca tgattcatta gaaaatcacc cttttgagcc gtatccctta
     1741 tctttgatac aggataagcc tattttatgt ggtgtttgtc taaaactact aacatataag
     1801 caatataaag aaagtttaag ttgccccttt tgtttttctc gctttaatcc aggttgccaa
     1861 aatcataagg aacgctattt taaatagcaa atcatctagt tttgaagtag gagaaaactc
     1921 aattccaaga gaaaatgaag taaatcttcc cacaataaaa cgcataatat caagattgtt
     1981 caatacctga tactatgcgt ttttaagatt ttaaagactt ttttccttta tctggtattt
     2041 tgactacttg ttaaaactgg gttaattttc aactgtttaa cagttattat gcaaagtcta
     2101 aaagattaga attgtcaaaa caatccgtct agagtatgcg tgatgccaac cgtggtggat
     2161 gttctcagtc atgccgttgg aagtacgacc tttacgatat gccatttggg aaagaacgta
     2221 agagtttgca gggtgagatt ccagaagaat tttcaatgtc agccgttgac atgtctatga
     2281 ttgaccacat tccagatatg attgaaaatg gtgtggacag tctaaaaatc gaagaacgta
     2341 tggagtctat tcactatgta ttaacagtaa ccaactgcta caaggcggct gtggatgcct
     2401 atcttgaaag tcctgaaaag tttgaagcta tcaaacaaga cttggtggac gagatgtgga
     2461 aggttgccca acgtgaactg gctacaggat tttactatgg tacaccatct gaaaatgagc
     2521 agttgtttgg tgctcgtcgt aaaatccctg agtacaagtt tgtcgctgaa gtggtttctt
     2581 atgatgatgc ggcacaaaca gcaactattc gtcaacgaaa cgtcattaac gaaggggacc
     2641 aagttgagtt ttatggtcca ggtttccgtc attttgaaac ctatattgaa gatttgcatg
     2701 atgccaaagg caataaaatc gaccgcgctc caaatccaat ggaactattg actattaaag
     2761 tcccacaacc cgttcaatca ggagacatgg ttcgagctct taaagagggg cttatcaatc
     2821 tttataagga agatggaacc agcgtcacag ttcgtgctta atgtagttgt ttagttttaa
     2881 aaaattatgc aaagctccat atacaacact taaacgagat taaagaatgg cgaaatccct
     2941 tgatgcgcaa gagattagct gtcctttttt attttttaag tgataaagtc ggagcttagg
     3001 tatcaaagcc tatcaaatta aacaaagaag tgatgtctta gatattttga aaaaaattaa
     3061 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattttacaa
     3121 aatgacattt atatattgca ttaagttaga tatatgatat aatgttgtta aaaagaggcg
     3181 caacttttta aactcaataa gaatcaaaga gcaaaccatg aatattaatg gaggaataaa
     3241 aaatgtaagt tataaatgta agtaagcatt atggtcattc aatcattctc aaagatataa
     3301 attttgcact taacaagggt gaaattgttg gtctagtagg gagaaatgga gttggtaaga
     3361 gtacattgat gaaaattctt gttcagaata atcaaccgac ttcagggaat attataagta
     3421 gtgataatgt tgggtattta attgaagaac caaaattatt tttatctaaa acaggtttag
     3481 agaatttaaa atatctggca aacttatatg gtgttgacta caatcaagaa agatttggga
     3541 gtttgattca agaattagat ttgactcagt ctattaacaa aaaagtaaag acctattctt
     3601 tgggtacaaa acaaaaatta gctttgcttc taactctcgt tacggaacct gatatattga
     3661 ttttagatga accgactaat ggtttagata ttgaatcatc acaaatagtt ttagcggttc
     3721 taaaaaattt agctttacat gaaaatgtgg gaattttaat atcgagtcat aaattagaag
     3781 atattgaaga aatttgtgag agggttcttt tcttggagaa cgggcttttg acatttcaaa
     3841 aagtagggaa agatagccat aattgcttgt ttgagatagc ttttttatca gctacagata
     3901 gagacatttt cattaccaaa caagaatttt gggatattgt tcaggaagag gggctgagaa
     3961 ttactatgtc tgggaatatt caaagtagtg agctttttaa attttttaac gaaaactcta
     4021 ttaaagtagt tgattttgaa actaaaaaag agacgctt
//
LOCUS       DAVGJJ010000109         3972 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000109 DAVGJJ010000000
VERSION     DAVGJJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.109"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            171..1616
                     /gene="cps4A"
                     /locus_tag="VVE35_001882"
     CDS             171..1616
                     /gene="cps4A"
                     /locus_tag="VVE35_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU3062011.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFILFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVIAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVSIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGIDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1618..2349
                     /locus_tag="VVE35_001883"
     CDS             1618..2349
                     /locus_tag="VVE35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU3062012.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYNALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2358..3050
                     /gene="cpsC"
                     /locus_tag="VVE35_001884"
     CDS             2358..3050
                     /gene="cpsC"
                     /locus_tag="VVE35_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU3062013.1"
                     /translation="MKEQNTLEIDVLQLFRVLWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPADTRIVSVSVNDRVPEEASRIANSLREVAAKKIISITRVSDVT
                     TLEEARPAISPSSPNLKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIEDTLQMT
                     LLGVVPNLNKLK"
     gene            3060..3743
                     /gene="cpsD"
                     /locus_tag="VVE35_001885"
     CDS             3060..3743
                     /gene="cpsD"
                     /locus_tag="VVE35_001885"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU3062014.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVS
                     PGEGKTTTSVNIAWSFARAGYKTLLIDGDIRNSVISGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITRKCDASILVTATGEANKRDVQKAQQQLKQTGKLFLGVVFNKLDISVDKYGVY
                     GFYGNYGKK"
BASE COUNT         1269 a          638 c          826 g         1239 t
ORIGIN      
        1 gatttgactg tcctgatcga tttgtcatat tcttatttca ttttactata tttttgtttc
       61 gcgggaagtc tactaagata cttaaagatg cagatagtga aaataaaggt gtagacatta
      121 ccgtaaaaaa gtgatataat cgtatgatgt tcaaggtata ggtgttaatc atgagtagac
      181 gttttaaaaa atcacgttca cagaaagtga agcgaagtgt taatatcgtt ttgctgacta
      241 tttatttatt gttagtttgt tttatattgt tcttaatctt taagtacaat atccttgctt
      301 ttagatatct taatctagtg gtaactgcgt tagtcctact agttgccttg gtagggctac
      361 tcttgattat ctataaaaaa gctgaaaaat ttactatttt tctgttggtg ttctctatcc
      421 ttgtcagctc tgtgtcgctc tttgcagtac agcagtttgt tggactgacc aatcgtttaa
      481 atgcgacttc taattactca gaatattcaa tcagtgtcgc tgttttagca gatagtgaga
      541 tcgaaaatgt tacgcaactg acgagtgtga tagcaccgac tgggactgat aatgaaaata
      601 ttcaaaaact actagctgat atcaagtcaa gtcagaatac cgatttgacg gtcaaccaga
      661 gttcgtctta cttggcagct tacaagagtt tgattgcagg ggagactaag gccattgtcc
      721 taaatagtgt ctttgaaaac atcatcgagt cagagtatcc agactacgca tcgaagataa
      781 agaagattta taccaaggga ttcactaaaa aagtagaagc tcctaagacg tctaagaatc
      841 agtctttcaa tatctatgtt agtggaattg acacctatgg tcctattagt tcggtgtcgc
      901 gatcagatgt caatatcctg atgactgtca atcgagatac caagaaaatc ctcttgacca
      961 caacaccacg tgatgcctat gtatcaatcg cagatggtgg aaataatcaa aaagataaat
     1021 taacccatgc gggcatttat ggagttgatt cgtccattca caccttagaa aatctctatg
     1081 gagtggatat caattactat gtgcgattga acttcacttc tttcttgaaa atgattgact
     1141 tattgggagg gatagatgtt cataatgatc aagagttttc agctctacat gggaagttcc
     1201 atttcccagt agggaatgtc catctagact ctgagcaagc tttaggtttt gttcgtgagc
     1261 gctactcctt agcagatggc gatcgtgacc gtggtcgcaa ccaacaaaag gtgattgtgg
     1321 ctatccttca aaaattaacg tcaaccgaag cgctgaaaaa ttatagtacg atcattgata
     1381 gcttgcaaga ttctatccaa acaaatatgc cacttgagac catgataaat ttggtcaatg
     1441 ctcagttaga aagtggtgga acttacaaag tgaattcgca agacttgaaa ggtacaggtc
     1501 ggatggatct tccttcttat gcaatgccag acagtaacct ctatgtgatg gaaatagatg
     1561 atagtagttt agctgtagtt aaagcagcta tacaggatgt gatggagggt agatgaaatg
     1621 atagacatcc attcgcatat cgtttttgat gtagatgacg gtcccaaatc aagagaggaa
     1681 agcaaggctc tcttggcaga agcctacaga cagggggtgc gaaccattgt ctctacctct
     1741 caccgtcgca agggcatgtt tgaaactccg gaagagaaga tagcagaaaa ctttcttcag
     1801 gttcgggaaa ttgcaaaaga agtagcagat gatttagtca ttgcttatgg cgcagagata
     1861 tactatactc tggatgctct agaaaagcta gaaaaaaaag aaattcctac ccttaatgat
     1921 agtcgttatg ccttgattga gtttagcatg catacttcct atcgtcagat tcatacggga
     1981 ttgagcaata ttttgatgtt gggaatcacg ccagtaattg ctcatattga acgttataat
     2041 gctttagaga ataacgaaaa acgtgttcgt gaactgattg atatggggtg ctatactcag
     2101 ataaatagtt atcatgtttc aaaacctaag ttctttggtg aaaaatataa attcatgaaa
     2161 aagagagctc ggtatttttt ggaacgtgat ttagttcatg tagttgcaag tgacatgcac
     2221 aatttagaca gtagacctcc atatatgcaa caggcatatg atatcattgc taagaaatat
     2281 ggagcgaaaa aagcgaaaga actttttgta gataatccca gaaaaattat aatggatcaa
     2341 ttaatttagg agaaaatatg aaggaacaaa acactttgga aatcgatgta ttgcaactat
     2401 tcagagtttt atggaaaaga aagttggtca ttttattagt ggcaattata acttcttcag
     2461 ttgcttttgc ctacagtact tttgttatca aacctgagtt tactagtacg actcggattt
     2521 atgtagttaa ccgtaatcag ggagagaagt ctggtttaac caatcaagac ttgcaggcag
     2581 gatcgtactt ggttaaagac tatcgtgaaa ttatcctatc gcaggatgtt ttggaggaag
     2641 ttgtttctga tttgaaacta gatttgacgc caaaaggttt ggctaataaa atcaaagtaa
     2701 cagtaccagc tgatacccgt attgtctctg tttcagttaa tgatcgagtt cctgaagagg
     2761 caagccgtat cgctaactcc ttgagagaag tagctgctaa aaaaattatc agtattactc
     2821 gtgtttctga tgtgacaaca ctggaggagg caagaccggc gatatcaccg tcttcgccaa
     2881 atcttaaacg caatacacta attggttttt tggcaggggt gattggaact agtgttatag
     2941 ttcttcttct tgaacttttg gacactcgtg tgaaacgtcc ggaagatatc gaagatacac
     3001 tacagatgac acttttggga gttgtaccaa acttgaataa gttgaaatag gagagagaga
     3061 tgccgacatt agaaatagca caaaaaaaac tggagttcat taagaaggca gaagaatatt
     3121 acaatgcctt gtgtacaaat atacagttga gcggagataa actaaaagta atttccgtta
     3181 cttctgttag cccgggggaa ggaaaaacaa ctacttccgt aaatatagca tggtcgtttg
     3241 cgcgtgcagg ctataaaact cttttgatcg atggcgatat tcgaaattca gtcatatcag
     3301 gattttttaa atctcgtgaa aaaattacag ggctaacaga atttttatct gggacagctg
     3361 atttatctca cggtttatgt gatacaaata ttgaaaattt atttgtagtt caatcgggct
     3421 ctgtatcacc aaaccctaca gccttgttac aaagtaaaaa ttttaatgat atgattgaaa
     3481 cattgcgtaa atattttgat tatatcattg ttgatacagc acctattgga attgttattg
     3541 atgcggcaat tatcactcga aagtgtgatg cgtccatctt ggtaacagca acaggtgagg
     3601 cgaataaacg tgatgtccaa aaagcgcaac aacaattaaa acaaacaggg aaactgttcc
     3661 taggagttgt ttttaataaa ttagatatct cggttgataa gtatggagtt tacggtttct
     3721 atggaaatta tggtaaaaaa taacttagga aagattttat ggatgaaaaa ggattgaaaa
     3781 tttttctggc agtattacag agtattattg tcattttatt ggtttatttt cttagctttg
     3841 ttagagagac agaacttgaa cgttcttcga tggttatact ataccttctc cacttttttg
     3901 tattctattt tagttcctat ggtaacaatt tttttaaaag agggtaccta gttgagttta
     3961 atagtactat aa
//
LOCUS       DAVGJJ010000110         3940 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000110 DAVGJJ010000000
VERSION     DAVGJJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.110"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            77..304
                     /locus_tag="VVE35_001886"
     CDS             77..304
                     /locus_tag="VVE35_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062015.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            592..3537
                     /gene="nanA"
                     /locus_tag="VVE35_001887"
     CDS             592..3537
                     /gene="nanA"
                     /locus_tag="VVE35_001887"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU3062016.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESG
                     RNGNPNKYGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DRVTITNLRDNPKASDPSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQK
                     EEAYKKIDGKTYQILYREGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGD
                     LYKGNQLLGNIYFTTNKTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKF
                     LGVGPGTGIVLRNGPHKGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGPTAFFLGL
                     FTLGKKREQ"
BASE COUNT         1340 a          658 c          881 g         1061 t
ORIGIN      
        1 ttgctgtaaa aatagtagta gctatgcgaa ataacagata gagagaaggg attgaagctt
       61 agaaaagggg aataatatga tatttaaggc attcaagaca aaaaagcaga gaaaaagaca
      121 agttgaacta cttttgacag tttttttcga cagttttctg attgatttat ttcttcactt
      181 atttgggatt gtccccttta agctggataa gattctgatt gtgagcttga ttatatttcc
      241 cattatttct acaagtattt atgcttatga aaagctattt gaaaaagtgt tcgataagga
      301 ttgagcagga agtatggtgt aaatagcata agctgatgtc catcatttgc ttataaagag
      361 atattttagt ttaattgcag tggtgtcctg gtagataaac tagattggca ggagtctgat
      421 tggagaaagg agaggggaaa attggcacca atttgagata gtttgtttag ttcatttttg
      481 tcatttaaat gaactgtagt aaaagaaagt taataaaaga caaactaagt gcattttctg
      541 gagtaaatgt cttatttcag aaatcgggat atagatatag agaggatcag tatgaatcgg
      601 agtgttcaag aacgtaagtg tcgttatagc attaggaaac tatcggtagg agcggtttct
      661 atgattgtag gagcagtggt atttggaacg tctcctgttt tagctcaaga aggggcaagt
      721 gagcaacctc tggcaaatga aactcaactt tcggggggga gctcaaccct aactgataca
      781 gaaaagagcc agccttctga ggaaggagaa aatataactc ttcctgcaga gcatgtagag
      841 tctgtttcag agactgaact ttctggcaat aagcaagaac aagaaaggaa agataagcaa
      901 gaagaaaaga ttccaagaga ttactatgca cgagatttgg aaaatgtcga aacagtgata
      961 gaaaaagaag atgttgaaac caatgcttca aatggtcaga gagttgattt atcaagtgaa
     1021 ctagataaac taaagaaact tgaaaacgca acagttcaca tggagtttaa gccagatgcc
     1081 aaggccccag cattctataa tctcttttct gtgtcaagtg ctactaaaaa agatgagtac
     1141 ttcactatgg cagtttacaa taatactgct actctagagg ggcgtggttc ggatgggaaa
     1201 cagttttaca ataattacaa cgatgcaccc ttaaaagtta aaccaggtca gtggaattct
     1261 gtgactttca cagttgaaaa accgacagca gaactaccta aaggccgagt gcgcctctac
     1321 gtaaacgggg tattatctcg aacaagtctg agatctggca atttcattaa agatatgcca
     1381 gatgtaacgc atgtgcaaat cggagcaacc aagcgtgcca acaatacggt ttgggggtca
     1441 aatctacaga ttcggaatct cactgtgtat aatcgtgctt taacaccaga agaggtacaa
     1501 aaacgtagtc aactttttaa acgctcagat ttagaaaaaa aactacctga aggagcggct
     1561 ttaacagaga aaacggacat attcgaaagc gggcgtaacg gtaacccaaa taaatatgga
     1621 atcaagagtt atcgtattcc agcacttctc aagacagata aaggaacttt gatcgcaggt
     1681 gcagatgaac gccgtctcca ttcgagtgac tggggtgata tcggtatggt catcagacgt
     1741 agtgaagata atggtaaaac ttggggtgac cgagtaacta ttaccaactt acgtgacaat
     1801 ccaaaagctt ctgacccatc gatcggttca ccagtgaata tcgatatggt gttggttcaa
     1861 gatcctgaaa ccaaacgaat cttttctatc tatgacatgt tcccagaagg gaagggaatc
     1921 tttggaatgt cttcacaaaa agaagaagcc tacaaaaaaa tcgatggaaa aacctatcaa
     1981 atcctctatc gtgaaggaga aaagggagct tataccattc gagaaaatgg tactgtctat
     2041 acaccagatg gtaaggcgac agactatcgc gttgttgtag atcctgttaa accagcctat
     2101 agcgacaagg gggatctata caagggtaac caattactag gcaatatcta cttcacaaca
     2161 aacaaaactt ctccatttag aattgccaag gatagctatc tatggatgtc ctacagtgat
     2221 gacgacggga agacatggtc agcgcctcaa gatattactc cgatggtcaa agccgattgg
     2281 atgaaattct tgggtgtagg tcctggaaca ggaattgtac ttcggaatgg gcctcacaag
     2341 ggacggattt tgataccggt ttatacgact aataatgtat ctcacttaaa tggctcgcaa
     2401 tcttctcgtg tcatctattc agatgatcat ggaaaaactt ggcatgctgg agaagcggtc
     2461 aacgataacc gtcaggtaga cggtcaaaag atccactctt ctacgatgaa caataaacgt
     2521 gcgcaaaata cagaatcaac ggtggtacaa ctaaacaatg gagatgttaa actctttatg
     2581 cgtggtttga ctggagatct tcaggttgct acaagtaaag acggaggagt gacttgggag
     2641 aaggatatca aacgttatcc acaggttaaa gatgtctatg ttcaaatgtc tgctatccat
     2701 acgatgcacg aaggaaaaga atacatcatc ctcagcaatg caggtggacc gaaacgtgaa
     2761 aatgggatgg tccacttggc acgtgtcgaa gaaaatggtg agttgacttg gctcaaacac
     2821 aatccaattc aaaaaggaga gtttgcctat aattcgctcc aagaattagg aaatggggag
     2881 tatggcatct tgtatgaaca tactgaaaaa ggacaaaatg cctataccct atcatttaga
     2941 aaatttaatt gggaattttt gagcaaaaat ctgatttctc ctaccgaagc gaaagtgaag
     3001 cgaactagag agatgggcaa aggagagatg ggcaaaggag ttattggctt ggagttcgac
     3061 tcagaagtat tggtcaacaa ggctccaacc cttcaattgg caaatggtaa aacagcgact
     3121 ttcctaaccc agtatgatag caagaccttg ttgtttgcag tagataagga agatatcgga
     3181 caggaaatta ttggtatagc taaaggaagc atcgaaagta tgcataatct tcctgtaaat
     3241 ctagcaggtg ccagagttcc tggcggagta aatggtagca aagcagcggt gcatgaagtt
     3301 ccagaattta cagggggagt taatggtaca gagccagctg ttcatgaaat cgcagagtat
     3361 aagggatctg attcgcttgt aactcttact acaaaagaag attatactta caaagctcct
     3421 cttgctcagc aggcacttcc tgaaacaggg aacaaggaga gtgacctcct agcttcacta
     3481 ggaccaacag ctttcttcct tggtctgttt acgctaggga aaaagagaga acaataagag
     3541 aagaattcta aacatttgat tttgtaaaaa tagaaggaga tagcaggttt tcaagcctgc
     3601 tatctttttt tgatgacatt caggctgata cgaaatcata agaggtctga aactactttc
     3661 agagtagtct gttctataaa atatagtaga ttgaaataag atgtgaacaa ctctatcagg
     3721 aaagtcaaat taatttatag aattatttta gcagtcaagg tgtactgtta tagattcaat
     3781 atattatgtg actattaacc ttgtcttctc ctaagattga ctttcttgtt ttcttatctt
     3841 gtccactcga aacaagtatt gtaagaattt gattattttt gaaagtactt ttaatatact
     3901 tgatatagtt aaaaaagatt tgaaactaaa ttccaaatta
//
LOCUS       DAVGJJ010000111         3925 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000111 DAVGJJ010000000
VERSION     DAVGJJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.111"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            187..633
                     /gene="rplM"
                     /locus_tag="VVE35_001888"
     CDS             187..633
                     /gene="rplM"
                     /locus_tag="VVE35_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU3062017.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            652..1044
                     /gene="rpsI"
                     /locus_tag="VVE35_001889"
     CDS             652..1044
                     /gene="rpsI"
                     /locus_tag="VVE35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU3062018.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            1354..1563
                     /locus_tag="VVE35_001890"
                     /pseudo
     CDS             1354..1563
                     /locus_tag="VVE35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1551..1643
                     /locus_tag="VVE35_001891"
     CDS             1551..1643
                     /locus_tag="VVE35_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU3062019.1"
                     /translation="MFQLIINRYKKKSFYWYKEVIESNGETLYD"
     gene            1719..2057
                     /locus_tag="VVE35_001892"
     CDS             1719..2057
                     /locus_tag="VVE35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU3062020.1"
                     /translation="MTLTIHVYYKGDGDCAINFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            2247..3683
                     /locus_tag="VVE35_001893"
     CDS             2247..3683
                     /locus_tag="VVE35_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU3062021.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFAAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLDAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            3735..>3925
                     /locus_tag="VVE35_001894"
     CDS             3735..>3925
                     /locus_tag="VVE35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU3062022.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVS"
BASE COUNT         1276 a          600 c          808 g         1241 t
ORIGIN      
        1 cagttacttt gagaagttac cgaaaaatat ttttcatatc tattgacttt taggggtgaa
       61 atttggtatg atagtaggcg gtattgttta ccccatttga aaggccccgg aaccttccaa
      121 atacttttcg atgggaagga acacccatca ccgtaaacaa aaatcgaact atatatagga
      181 gaaatcatga acaaaacaac atttatggct aaaccaggcc aagttgaacg taaatggtac
      241 gtagttgacg caactgatgt accacttgga cgtctttctg cagtagttgc tagcgtactt
      301 cgcggaaaaa acaaaccaac atttacacca cacactgata caggtgactt cgtgattgtt
      361 atcaatgctg aaaaagttaa attgactggt aaaaaagcaa ctgataaaat ctactacact
      421 cactcaaacc acccaggtgg attgaaacaa atctctgcag gtgaacttcg ttctaaaaat
      481 gcagtacgtt tgattgagaa atcagttaaa ggtatgcttc cacacaatac acttggacgc
      541 gctcaaggta tgaagttgaa agtatttgtt ggagctgagc acactcacgc tgcacaacaa
      601 ccagaagttc ttgacatttc aggacttatc taaggaaagg aacaataaag tatgtcacaa
      661 gcacaatatg caggtactgg acgtcgtaaa aacgctgttg cacgcgttcg ccttgttcca
      721 ggaactggta aaatcactgt taacaaaaaa gatgttgaag agtacatccc acacgctgac
      781 cttcgtcttg tcatcaacca accattcgca gttacttcaa ctgtaggttc atacgacgtt
      841 ttcgttaacg ttgtaggtgg tggatacgct ggtcaatcag gagctatccg tcatggtatc
      901 gctcgtgccc ttcttcaagt agacccagac tttcgcgatt cattgaaacg cgcaggactt
      961 cttacacgtg actcacgtaa agttgaacgt aagaaaccag gtcttaagaa agctcgtaaa
     1021 gcatcacaat ttagtaaacg ttaattcgaa agaattacta tacttataca gagcaccttt
     1081 cggggtgttc tttttttata ctttcttact aaattggtgc aattgacaca gttgttggga
     1141 ctttagtcgc ttacaaatgt ggctgcaacc tgacaaggtc agttgcctca aaacgttaat
     1201 caatacgatt atatcaacgt ttcaaagcac tcaaaagttt accttatttt tatagtaagg
     1261 tgaaataaga ataggacaaa ccgaggatag tcaaatcgaa ttctaacaat gttttagaag
     1321 tagaggtgta ctattctagt ttcaatctac tatattatgg ataatgcaag attccataga
     1381 atgggtaagt tagaactctt gtgtgaagag tttgggtata aacttttacc tcttcctccc
     1441 tactcacctg agtacaatcc tattgagaaa acatgggctc atatcaaaaa gcacctcaaa
     1501 aaggtattac caagttgcaa taccttttac gaggctattt tgtcctgctc ttgtttcaat
     1561 tgattataaa tcgctataag aaaaaatcat tttattggta taaagaagtg attgaaagta
     1621 atggcgaaac cttatatgat tagttcataa aaaacaagtt tagattctta tctaagcttg
     1681 tttttgaaaa ctttagggat aattttggag gatatgagat gacattgact attcatgtat
     1741 attataaagg cgatggagat tgtgcaataa attttgcaag agaaatgctt gaatctgggc
     1801 ttgtcgcaga aattagatgt caaaaaggta atttgaaata cgaatatttt ttaccaattg
     1861 aaaaagaggg aactattctc cttattgatc agtggataaa tcaagaagct ttagatgaac
     1921 atcatcaatc aaagacaatg cagaaaatct tagatttaag aaaaaaatat cacttgcaga
     1981 tgcaagttga acgttatata gaagatgatt ctgggatgcc agaaagcgat aagtcgttta
     2041 tagacacagg gaattagaat tattgtgaga gtttttgtag tttgaaagta ttgaaaatcg
     2101 taaagatatc attacgagaa tacaagaaga tattgttcta tttccattaa aaattacttc
     2161 tttccttaac aataaggaaa aagaatgcgt tttcatcttt tggtttaagg tttataataa
     2221 gagtatcaaa gtcaggagga aaaaagatga ctacttttaa agatggattt ttatggggtg
     2281 gtgctgttgc tgctcatcaa cttgaaggtg gatggcaaga aggtggcaag ggaattagtg
     2341 ttgctgatgt tatgactgct ggtcgtcatg gagtagctcg tgaaattact ttgggagttt
     2401 tagagggtaa atattatcca aatcatgagg cgatagattt ttatcaccgt tataaagaag
     2461 acatagcact ttttgctgaa atgggattca agtgcttccg tacctctatt gcatggacac
     2521 gtatctttcc aaaaggtgat gagttagagc cgaatgaaga aggattacag ttttatgata
     2581 atctttttga tgaatgctta aagaatggta ttgaacctgt catcactcta tctcattttg
     2641 aaatgcctta tcacttagtg accgaatatg gtggttggaa aaataggaaa ttgattgatt
     2701 tctttgctcg ttttgcagaa gtcgtattta aacgttacaa agataaggtt aaatattgga
     2761 tgactttcaa tgaaatcaat aatcaagcga attatcagga agattttgca ccatttacta
     2821 actcaggtat tgtatatgag gaaggtgata atagagaagc aattatgtat caagcagcac
     2881 attacgaatt agttgcttct gcacgagctg taaaaattgg tcatgagatt aatccagatt
     2941 ttcaaatagg ttgtatgatt gcgatgtgtc caatttatcc agttacttgc aatcctaaag
     3001 atatcttaat ggcaatgaaa gctatgcaga agcgttatta ttttgctgat gtgcatgttt
     3061 taggtaaata tcctgagcat attttcaagt attgggaacg aaaaggtatt tcagttgatt
     3121 ttgctgccca ggataaagaa gatttacttg gtgggactgt agattacatt ggtttcagtt
     3181 actatatgtc ctttgctatc gactctcatc gtgaaaataa tccttatttt gattatcttg
     3241 aaacagaaga tttagtgaaa aataattatg ttaaggcttc tgaatgggag tggcaaattg
     3301 atccagaagg tttgcgttat gcgttaaatt ggtttacaga ccactatcac ttaccactct
     3361 ttattgttga aaatggtttt ggagctatag atcaagttgc agcagatggt atggtacatg
     3421 atgattatag aattgaatat ctagatgccc atattcgtga aatgaaaaag gctgtagttg
     3481 aagatggtgt tgatttaatg ggttatactc catggggatg tattgatttg gtttcagctg
     3541 gtaccggtga aatgcggaaa cgttatggct ttatttatgt agataaagat gataatggga
     3601 agggaagtta taatcgttcc ccgaaaaaat cttttggctg gtataaggaa gttatttcat
     3661 ctaacggtga atcagtagaa tagtataaac aaactgagat tattctcagt ttgtttagca
     3721 tattgaggtg acatatgatt ggaagtattg cagcaatttt aacaacattt gcatttttac
     3781 cacaagtttt tcgtgtagtt aagacaaagg acacaggatc tattgctcta ggtatgtatg
     3841 ttatgcaagt tattggtatt gcattgtggc ttgctcatgg tattcgtata ggagacttgc
     3901 ctttgatttt agcaaatagt gtttc
//
LOCUS       DAVGJJ010000112         3644 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000112 DAVGJJ010000000
VERSION     DAVGJJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.112"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(159..1574)
                     /locus_tag="VVE35_001895"
     CDS             complement(159..1574)
                     /locus_tag="VVE35_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU3062023.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAKLLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1587..3425)
                     /locus_tag="VVE35_001896"
     CDS             complement(1587..3425)
                     /locus_tag="VVE35_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU3062024.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3443..>3644)
                     /locus_tag="VVE35_001897"
     CDS             complement(3443..>3644)
                     /locus_tag="VVE35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU3062025.1"
                     /translation="RLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNY
                     LLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1216 a          742 c          486 g         1200 t
ORIGIN      
        1 ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatcc ccctataaaa
       61 taaagaggtt tagaatttga tgtcttccaa acctctttat ttcatattca atgagatgtt
      121 gccttaaccg tgataatatc taatcatcaa taaacaaact atttgaataa ggattctcca
      181 tttgattcaa tcactccttt ataccaagta aaggacattt tcttatatcg attgaatgtg
      241 ccatttccat catcatttcg atcaacataa atgagaccgt acctcttaga aagttgtgca
      301 gtggacatag aaacgcagtc aatacatccc caagacgtat agcccataat ttccacacca
      361 tcttgtagag cttcagctac ttgcaataaa tgttctttca tatactgaat tctataatca
      421 tcttggactg ttaagttatt aaattcatct tttattagtt gatctttagc acctaatccg
      481 ttttccacta taaataatgg aatttgataa cggtcataat atctatttaa aattatacgt
      541 agtccaattg gatcaatttg ccatccccac tcagaagact ctaaataagg atttactaag
      601 ccaccaataa tattcccttc tcctgaatta tactgtgttg gaagagcaga ttgagtcaca
      661 ctcatgtaat agctaaagga taaaaaatct acggtataat tttttaataa ctttgcatct
      721 tcagctgcaa actctatgtt aatgtcatgt tccttaaaat atctttttgc ataattcgga
      781 taataacctc taacatgcac atctgaaaat agataattta gattctcata ctcatgagtc
      841 gcccatacat cttttggatt tggagtcatt ggataagctg gcatagctaa taccatacac
      901 cccaccttaa actctgaatt aatctcacga gcaatttttg taaccaaact cgaggcgact
      961 aattcatgat gtatagcttg atataattct tgtttcgaaa gattctcctt aggtatatct
     1021 attcctccac tagtaaacgg taattccaaa acagagttta cttcgttaaa tgtaagccaa
     1081 tatttaactt tatctttata cctttctaaa actgttcgag caaatttttc ataaaaatga
     1141 atcattctcc tatcaatcca tccatgatat tttcttgcta aatataatgg agtctcatag
     1201 tgtgaaagag ttacaagtgg ctctatcccg tgagcatgta gttcatcaaa caattcatca
     1261 taatatttca acccagcttc gttaggttct tcctcatctc cttttggaaa aattctactc
     1321 catgcaatag aagtacgaaa aacattaaag cccatttcag aaaacaaggt tatatcttcc
     1381 ttatatttat gataaaaatc aatacctatc aattttaagt tatcttctgt aggattttct
     1441 gttgcttctc ctaatccacc tttgggtaac acatcctgaa ctgataagcc cttaccatct
     1501 tcattatatg ctccctctac ttgattagct gcaacagctc caccccaaag aaaatcatct
     1561 ggaaaaatgg tcataacttt cctccattat aatattacca gtaattcctt agaatgctcg
     1621 attgtctgat tattaggtaa tactaataca tctagaaaat cattggtatt cgttacaatt
     1681 actggtgtaa ctgtttcgta gcctttagtc ttgattaaat tcaagtccat ttcaaaaatc
     1741 aactgatttt tgaaaactct gtctccttct tctacatgac taataaaacc ttgacctttt
     1801 agctcaacag tatctaatcc aatatgaatt agtaactcaa caccctcatc actcttcaat
     1861 ccaattgcgt gcttagtcgg aaaaatattt gtaattttcc catcaaatgg tgcataaacc
     1921 ttaccttcac ttgggataat cgctaccccg tctccaatta gtttatctga aaatgtttta
     1981 tcctggacat cgcttaacgg aatgatttct cctgatatag gagaaaatat cattttttta
     2041 tttgaaactc cagctttaac ttctaaattg ctagaactct cttcttcatc gattccaaat
     2101 atataagcta atacaaaggt aataacaacc gaaataaccg ccacaattaa agcatttaca
     2161 atatttgatg gtacatcaga ataaataaat tgaggcaacg ctatcaaaga tgggacagca
     2221 aatagatacg ctttaacact agtaagacct gcaaataatc ccgctaatcc accaccaatc
     2281 atagctgcat aaagcggttt tttatatttt aaagtcacac catatactgc aggttcggta
     2341 atccctgcaa gtaaggctga gaaacctgct gcaaaagcaa tttgttttgt attattattt
     2401 ttactcttta atgcaacagc catcgaagca gccccttgag ctaagtttga ccctaacatt
     2461 gctggaagaa ttaatacgtc tggagtagca atagatgccg ccaaaaaaat aggtgcaaaa
     2521 gcccaatgca ttccagtcat aacaataaat ggcataatag caccaagaat agctaatgta
     2581 agccatccag ctacaccata catttgccca actagatttg ataatccttc accaacaatt
     2641 actccaatag gtccgactac aactaaggca atacagcttg atactaataa tactagcgta
     2701 ggttgcaaaa aactcttagt aacagctggt gttaatttag caattatttt ttcaatatat
     2761 ttcatcaacc aaaccataat aagaattgga acgactgatg aaccataact agctggtgtc
     2821 acaggtgcac caaataaact aagaggattc cctgattgca ccatttgaac aaaatttgga
     2881 tggagaagta cacctgctac agacatagct aatgtagatg ttacttttaa tttttgtgat
     2941 gcagaataag ctaataacag cggtaagaaa taatatggag catccccaaa aaatgtcaaa
     3001 aaagcaatag tctgagaatc tgattgcaat ataccaagca ttggtaaaat gattaccaag
     3061 actttcagca tacctccccc taacattgct ggaatgattg gagtcatgga accagcgata
     3121 tactcaatga ttctttctaa aatattccct ttgtgccctt gaacaactga atcggattca
     3181 aaattgccaa gtttaacgaa ttctttataa taattagcta catcattacc aagtataatt
     3241 tgatattgtc cattcttttt cataatacct attacacctg gtatcttctt cacatcatca
     3301 tcattgacta aattttcatc ttttaattct aatcttaaac gtgttacaca atgggtaact
     3361 ctattgacat ttttttcacc tccaattaca tcgaggattt tttgtacctt atctttataa
     3421 ctcatggtat tctcctattc tattaatcta aattttttgt taagcgacga atatgagcca
     3481 tcaaataaac taattcacta gaagtcagca aataattgta ctcagtttgt ataaacattg
     3541 ccacctgttc accacattca tattctctag gatatttatt tttcattaat gctaacaagt
     3601 cttcatcatc atcgtcacaa taagttgtat tttcaaccaa gcga
//
LOCUS       DAVGJJ010000113         3615 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000113 DAVGJJ010000000
VERSION     DAVGJJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.113"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..963
                     /locus_tag="VVE35_001898"
     CDS             <1..963
                     /locus_tag="VVE35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062026.1"
                     /translation="LAVVIFVGVNLIINKEINSLYLIGYLLAAMKIKDSLDAYKEGLM
                     EIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQ
                     GEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGKDIKEISTESLFDKVSIVFQDV
                     VLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAELSGGE
                     RQRLSIARAFLKDAPILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHRMKSIE
                     NADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTKMAEEFIY"
     gene            974..1558
                     /locus_tag="VVE35_001899"
     CDS             974..1558
                     /locus_tag="VVE35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU3062027.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1558..2253
                     /locus_tag="VVE35_001900"
     CDS             1558..2253
                     /locus_tag="VVE35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU3062028.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            2371..3606
                     /locus_tag="VVE35_001901"
     CDS             2371..3606
                     /locus_tag="VVE35_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062029.1"
                     /translation="MNSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQ
                     NPKTYFFNVNTTLELLFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEK
                     QILCIAASYIAGTKIIVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLM
                     DIVDRVFLIDKGKLKKTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIK
                     NLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGE
                     KLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGIKNFDEEKAKIILKDLGLDEFIER
                     HPMSLSGGQKQRLAIASVMCKTSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIF
                     IVSHDIEFLNEVADEIFEL"
BASE COUNT         1326 a          449 c          688 g         1152 t
ORIGIN      
        1 cttgctgttg tgatatttgt cggcgtaaat ctaattatta ataaagagat aaattctctc
       61 taccttatag gatatttact agctgctatg aagataaaag actctttaga tgcatataaa
      121 gagggcttga tggaaatatt ttatttatcg cccaaaatag aaagattaaa agaaattcaa
      181 aatcaagatt tacaagaagg cgatgactat agcttaaaaa aatttgatat tgatctaaaa
      241 gatgttgagt ttgcctacaa taaagacgca aaagttttaa atggtgtcag ttttaaagct
      301 aagcagggag aggtcactgc tttggtaggt gcaagtggct gcggtaaaac aactatcttg
      361 aaacttatat caagacttta tgattatgac aagggacaaa tcttaatcga tggcaaagat
      421 ataaaggaaa tatcaacaga atcccttttt gataaggtgt ctattgtttt ccaagatgtg
      481 gttctcttta atcaaagcgt tatggaaaat attagaatcg gtaagcaaga tgcaagtgac
      541 gaagaggtta aaagagcagc aaaacttgca aattgcacag attttataga aaaaatggat
      601 aaaggtttcg atacagttat tggtgaaaac ggagctgagc tatcaggagg agaaagacaa
      661 agattatcaa tagccagagc cttcttaaaa gatgcgccga tattgatctt agatgagata
      721 acagcaagcc ttgatgttaa caacgagaaa aagattcaag agtctttaaa taatttagtt
      781 aaagataaaa ctgttgtaat catttcacat agaatgaaat ccatagaaaa tgcagacaag
      841 atagtagttc ttcaaaacgg aagagtagaa agcgaaggta agcatgaaga gcttttacaa
      901 aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa tggcagaaga atttatttat
      961 taggaggact acaatggata ataaaaaatt aaaagtaaaa gatttagtaa gtatcggtgt
     1021 ttttggcgta atttattttg ccttcatgtt tggagttggt atgatgggct tgattccaat
     1081 attgttctta atatacccga cagtattagc catagttgca ggaactgttg ttatgttatt
     1141 tatggctaag gttcaaaagc catgggcact atttatattt ggtatgatat caccacttgt
     1201 gatgtttgca gctggtcata cctacgtagt tgtggtttta tcacttatag taatgataat
     1261 agcagaatta attagaaaga ttggtaatta taattcattt aaatacaata tgctttctta
     1321 tgcaatcttc agcacatgga tatgtagctc tttaatgcaa atgcttttag caaaagaaaa
     1381 atatatggag tggtctttga tgacgatggg aaaagattat gttgatgtat tagaaaagtt
     1441 aataacttat cctcacatgg ctttagtagc cttaggtgct ttcttaggag gaattcttgg
     1501 agcatatata ggcaaggctc tattgaaaaa acactttgaa aaagcaggca tcgtataatg
     1561 cctacttttt cttcaaatgc tccctttaat cccaatccga tctctaagat attagtcgta
     1621 tttatcacag gcttaactgt tatgcatagc ataaacatcc gttttgagct ggcaattgtt
     1681 tgtcttattg gtattttgct ttatttgaat ggatacaaaa aaacactttt caaatggatt
     1741 atattgtgtg ggatacttta ctccttgcct aattttatgg tgttatctga attaaaccct
     1801 atagttaaga tgtttttgag tatacctatt gttattagaa tgtttatttt accatttatg
     1861 gcagcaagct ttatgataaa gacctcggat gtaggcgcaa taatttcatc gatggataag
     1921 cttaagattt caaagaatgt atccatacct attgcggtta tgtttagatt cttcccatct
     1981 tttaaggagg agaagaaaaa catcaaaata gctatgagag taagagggat aaattttaaa
     2041 aacccagtca aatatcttga atatgtttct gtgccactac tcattatatc atctaatata
     2101 tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag aaaatccaat tgccaagacc
     2161 agatacattc gcgtaaagat acagctaatt gattttgttt atgttttagc ggttgttgga
     2221 cttattgtgg gaggcttaat atggttgaaa taaaaaattt aagtcttgat tatggtgaag
     2281 agcatatatt agatgatata tcactatcca tagccgaggg agagtgcgtg ctatttacag
     2341 gaaaaaagtg gaagtggtaa gtcatcttta ataaattcaa tcaatggact agctgtaagg
     2401 tatgataacg caaagacaaa gggcgaaata attattgatg gtaagaatat aaaaaatttg
     2461 gaactttatc aaatctcaat gcttgtttca actgtttttc aaaatcctaa gacatatttt
     2521 tttaatgtca atacgacatt agaattatta ttttatttgg aaaatatcgg tcttgcaaga
     2581 gaagagatgg acaggcgttt gaaggatatg cttgagatat tcccgataaa aaatcttttg
     2641 aacagaaata tatttaatct atccggcggt gaaaaacaaa ttctttgcat tgcagcttct
     2701 tatatagcag gtacaaagat tatagttatg gatgagtctt catcgaattt agatattaaa
     2761 agcataagtg ttttggcaaa gatgctaaag atattaaaag agaaaggcat aagcataatt
     2821 gttgcagagc atagaattta ttatttgatg gacatagttg accgtgtatt tttaatagat
     2881 aaaggaaagc ttaaaaaaac ttatactaga agtgaatttt taaagctaga taaaaatgaa
     2941 ttaaatgctt taagtttaag agataaagaa ttaagtaaat taaaagttcc ttatttaaaa
     3001 gaaggtggag agtatcagat aaaaaatctt agctacaaat ttactgatga tgagtgttta
     3061 agcttaaaag atatttcatt caagcttggg aaaatttatg gcataatagg atccaacgga
     3121 cgaggaaaat caacgctttt aagatgttta ataggtcttg agaaaaaatc aaaagaagaa
     3181 atttatttta agggagagaa gctatctaaa aaagaaagac tcaaaaactc ttcacttgtt
     3241 atgcaagatg taaatcatca attattcaca gatgaggtat tcaacgagct tagattagga
     3301 ataaagaatt ttgatgaaga aaaggcgaaa atcattttaa aagatttagg cctggacgaa
     3361 tttattgaaa ggcatccgat gagtttatca ggagggcaaa agcaaaggct tgcaatagca
     3421 tctgttatgt gcaagacttc tccatttatc ttttttgacg aaccttcaag tggtatggat
     3481 tattccaata tgataaaaat atctgaactg attaataagt ataaaaccat ggataaaata
     3541 atttttattg tttcccatga tatagaattt ttaaatgaag tggcagatga aatttttgaa
     3601 ttataaaata gagtc
//
LOCUS       DAVGJJ010000114         3516 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000114 DAVGJJ010000000
VERSION     DAVGJJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.114"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            24..1034
                     /locus_tag="VVE35_001902"
     CDS             24..1034
                     /locus_tag="VVE35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062030.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            1046..2737
                     /locus_tag="VVE35_001903"
     CDS             1046..2737
                     /locus_tag="VVE35_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU3062031.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            2740..3450
                     /locus_tag="VVE35_001904"
     CDS             2740..3450
                     /locus_tag="VVE35_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU3062032.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1078 a          511 c          670 g         1257 t
ORIGIN      
        1 tagtaataga gggagttagt gagatgatta aatttgataa tattcaaatt aaatatggtg
       61 attttgttgc aattgataat ctgaatttag atatacatga aggggaattc tttacatttc
      121 ttgggccttc aggatgtggt aaatcaacta ctttgagagc attggtaggt tttttagatc
      181 catcatcagg aagtattgaa gttaatggaa cagatgtcac tcatttggaa cctgaaaagc
      241 gtggaattgg tattgtattt caatcttatg cgctatttcc aactatgact gtttttgata
      301 atattgcatt tggtttaaaa gttaagaagg tagctccaga tgttattaaa gctaaagtat
      361 cagcagtggc agcaaaaatt aagatctctg atcaacagtt acagcgtaat gtatcagaat
      421 tatctggggg tcaacaacaa agggtagcat tggctcgtgc tctggttctt gaacctaaaa
      481 ttctttgtct agatgaacca ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa
      541 aagagttgaa aagacttcaa aaagagttag gtattactac tttatatgtt actcatgatc
      601 aagaggaagc cttgacttta tctgatagaa ttgcagtctt taacaatgga tacatcgaac
      661 aggtcggtac accagtagag atttatcata attctcaaac tgaatttgta tgtgatttta
      721 ttggagatat taatgttttg accgatgaaa cagtccacga agtattactg aaaaatacaa
      781 gcgttttctt agaggataaa aaaggttaca ttcgattaga gaaagttcga ttcaatcgtg
      841 aaactgaaca agattttatt ctaaaaggga caattattga tgttgagttt tctggagtta
      901 caattcacta tacaataaaa gtttctgaaa gtcagattct taatgtaaca agtattgata
      961 gtcaggctgc tattagatct gtcggagaaa gtgtggaatt atttatcaca ccatcagacg
     1021 ttctgcaatt ttaaggaggt gcagtatgcg tcataaatta aatttaaaag attggcttat
     1081 tcgtttaggg ttaatctggt tcttagtaac atttattatt tatccaaact ttgatctagt
     1141 agtgaatgta tttgtaaaag gaggagaatt ttcccttgat gctgtacatc gtgttctaaa
     1201 atctcagagg gcacttcaga gtattatgaa cagttttaag ttagcatttt cacttattat
     1261 tacagttaat gtcgtaggta ttctttgtgt tctatttaca gagtactttg atattaaagg
     1321 tgctaaaatt ttaaaattag gttatatgac ctctttaatt tatggaggag tggttttagc
     1381 gactggatat aaatttgtct atggtcctta tggattgatt acaaaatttt tacaaaatgt
     1441 tatcccttct ttagactcta actggtttat tgggtatggt gcagtcttat tcattatgac
     1501 attttcagga actgctaatc atacattgtt tttaacaaat acaattcgaa gcgttgacta
     1561 tcacactatt gaggctgctc gaaatatggg agcaaaacca tttactgttt tccgaaaagt
     1621 agtgttacca accttaattc caactctatt tgcacttact attatggttt ttcttagtgg
     1681 tttatctgca gtagcagcac ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc
     1741 aatgattatt acatttgcag ggatggggaa ttctcgtgat ttagctgcct tacttgcaat
     1801 tattttaggt attgcaacta caattttgct tactatcatg aataagatag aaaaaggtgg
     1861 aaattatatt tctatctcta agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa
     1921 gccttggaat atcattgctc acattgtagc atatggattg ttcacagttt tcatgcttcc
     1981 actaattttt atagtattat actcatttac agatccagtt gcaattcaaa caggtaactt
     2041 aacattatca aactttactt tagaaaatta tcgcttattc tttagtaata gtgcggcatt
     2101 ctctccattc ttggtcagct ttatttattc tattattgct gcgacaacag caacaattct
     2161 cgcagttgta tttgctcgtg ttgtcagaaa acataaatct cgttttgatt tcttatttga
     2221 atatggtgct ctacttcctt ggttactacc aagtacactt ttagcagtaa gtttattatt
     2281 tacttttaat cagccacaat ttcttgtctt gaatcagatt ttggtaggta gtttggtaat
     2341 tctacttatt gcatatatag ttgtaaaaat cccattttct tatagaatgg tacgtgctat
     2401 tttatttagt gttgatgatg agatggaaga tgcagcaaga agtatgggtg cttcaccttt
     2461 ttatactatg atgaaggtta tcattccatt tattttaccg gttgttctct ctgttattgc
     2521 tttaaacttt aactctttat taactgactt cgacttatct gtattccttt accatcccct
     2581 agctcaacca ttaggtatta cgattcgatc tgcaggtgat gaaacagcaa catctaatgc
     2641 acaagctctg gtatttgttt atacaattgt tctgatgatt atttctggaa cggtattata
     2701 cttcacacaa agaccggggc gtaaagtaag gaaataatca tgacagccac tagtcttggg
     2761 ttatcaaata ttgaaatagt tgtcaggatt gttttatcag tagtcattgg tagtataatt
     2821 ggtttagaga gagggagcaa atcccagcct gcaggcatcc gaacttatag tattgtttgt
     2881 ctagctgcat gtttgattat gatgacgaat gaatacgtat cttataaatt tgggacagga
     2941 gatcctacac gattaggagc tcaagttata tcaggtgtgg gttttctagg cgctggaacg
     3001 attcttatta cagataaaaa gaaaattaca ggtctgacaa ctgcagcagg catttgggct
     3061 tcggcaggaa ttggattagc tattggagta ggtttttatg agggagctct tttagtagcc
     3121 atttctgttt ggggtgtgat atccatgttc caaccactaa aaaaatatct gcaaaatcgt
     3181 tctaaaatga ttgaattgta tatagtagtt aaatctacag aagcctataa tcgtgtattg
     3241 gtttattgtg cagaaaatgg tatcagaatg accgattcaa gaacagcgtt tggagatgtt
     3301 aattctgaca gaattgaata ctttgatgtt ccggacaaaa aaatagcgtc atttattact
     3361 ctggaattat caggtagatt tgaacatctt cgactgatgg aagaaattgc taatattgta
     3421 ggtgttattt atgtcgaaga aattagctga aatttagtaa acaaagggac agatgttttt
     3481 gtccttttgt tttttagatg ttttaatcat ctaatt
//
LOCUS       DAVGJJ010000115         3355 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000115 DAVGJJ010000000
VERSION     DAVGJJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.115"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            207..401
                     /locus_tag="VVE35_001905"
     CDS             207..401
                     /locus_tag="VVE35_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062033.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNDTGKSTLL"
     gene            complement(753..2063)
                     /locus_tag="VVE35_001906"
     CDS             complement(753..2063)
                     /locus_tag="VVE35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU3062034.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2300..3172
                     /locus_tag="VVE35_001907"
     CDS             2300..3172
                     /locus_tag="VVE35_001907"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU3062035.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         1045 a          545 c          740 g         1025 t
ORIGIN      
        1 actgacgaga agtttttgag tcttttcttg taacacatat agtatactgt ggttagaata
       61 gtagactgtg acttctaaca aattgctaga aatgaatttc aatctcccaa tttatttaat
      121 atattatttt aatatattaa ataaattctg gatcataatc atttaaaaaa attttatttt
      181 tttatttttc attacgaata atatagatga aggggaaaga gtatgaaaac agaactgttt
      241 cttttgctat tagttcaaaa ggagaaaaaa tgaaagtaga aaatatttcg tatagggtgg
      301 atcatcgtat attgtttgat aatatttctt ttgatacttc gagttcaggc gtgacattaa
      361 ttactggtaa aaatgataca ggaaagtcaa ctttactata gtagattgaa actagaatag
      421 tacacctcta cttctaaaat attgttagaa atcgatttga ttgtcctgtt cttatttcat
      481 tttactacat cttaaattga gtatgacgaa gtgcgctccc atgttctggg aacgcacttt
      541 cttcatattt ttcatattct tgaatccatc gataaagact attgggatga atttttaaag
      601 ttgaactaat catttttaca ggatgaggtt tacagcagag agtttgaagg ctttatcaaa
      661 ggtttttctt ggcataatga cttttcctcg tttccactta attttgtgtc tactttattg
      721 taccaagtcc acccttaagt tagataataa atctaactta aggaagctaa aaggatgaga
      781 atccaggtgg tcaagagtcc caaacttaag ctgatgggga cacccagaat aatttgcttt
      841 ttgaaggcaa ggccgcgttc ctctatattg ggaagtgaga gttgaatgag agaaccagct
      901 gatgaaaagg gtgagatatt agtagataga gcgccaataa cggtggctgt tgtgagtaag
      961 tgaatatcaa tctgaggatt ttgagcactg atgatagcaa tgatgggaaa gagggctgga
     1021 gctacaacgg atagggtgga actaaagagt gacatcactc cggctatcac acaaaagaac
     1081 agaggtaacc agaaatgagg aatggttgtt gtcatgaggt gccctatcag tgtgactaaa
     1141 cctgacttga ccgctagaga cattagtaag ctcatgccgc agagcatgat aattgtagcc
     1201 cagggaacct tagctaaaat ggcttcttgc ttccctaatt tgagccttaa ggcgaggcag
     1261 accatgagta ttgagacaaa gccaatatca aatgtttttt gataagtagc tatccaggcg
     1321 atgtttggga aaatgagatg caacaaggga aaaagccaaa ccaaaaccat gctgctgatc
     1381 atgagcaagg tggtttgtct ttgaaccttg ctgaggagtg gtggttggtc aatagtcaag
     1441 gatgagtttg ttcttccctt actatagtga ctgtaacagg ataataaaag caagacgatg
     1501 agtgggtaga taatgctgac gataaagata tgattgccaa gtgaaaaagc ttgctcttcc
     1561 catcccattt gcttaaacag gccttgaaag acaatgcctg agctactggt tatcaaatta
     1621 gcccctcctg aagctcccca attgacggct tgagctccaa tcaaagggtg tttgtccgct
     1681 ttttgacaga gggtaatcgc tagaggacag caaacggcca tagtagtgaa aaatccagca
     1741 cctaaagcag acaaaagggt tgccatcagg tataaaatca tgtagagggc gttagggtag
     1801 gtgcgtgtgc ggtagagaat gtgttgagcc aaaacatcaa gagtaccgtt agttattgca
     1861 acgttataaa agagagagac gctaaaaatg gtaaaaaaga gtgaggttgg ccaaaaatga
     1921 agaagttctt tggggcttaa tcccatgaga gtggttgcga tgaggtaaga aaaagcaata
     1981 gccagcaggc caatattgat tttggtgcgg taaccaattc caatggctag agcaatggcg
     2041 ctaatcatta ttaaatgaat cattgaattg tcctttcagt tagaatatgg aaataggttg
     2101 gcaattttgg cgctcaaaag accacctgtt tcctattggt acttgactat tatacacaaa
     2161 aaaaatacaa ttgtcttgac aattgtattg acacttgtta gaattaaaaa tgaggtattc
     2221 aggcttgtga tttcctatgg aagttaatag tgattgcctc taatgcttac aagtgatatt
     2281 aaaaatagag gacctagcga tgtcaatcat ttcaactgat ttaacccctt ttcaaataga
     2341 tgatacattg aaagcagcct tgcgagaaga tgttcattcc gaagattaca gtaccaatgc
     2401 catttttgat catcatggcc aagccaaggt gtcgcttttt gccaaggaag ctggtgtttt
     2461 agcggggcta accgtttttc aaagggtttt taccctattt gatgccgagg tgaccttcca
     2521 gaatcctcat caatttaagg atggggatcg tttgactagt ggcgatttgg ttttagaaat
     2581 cataggctcg gtgagaagtc tcttaacatg tgaacgcgtt gccttgaatt ttttacaaca
     2641 tttatcaggg attgcttcga tgacagctgc ttatgtagaa gccttaggcg atgattgcat
     2701 taaggtattt gatactcgaa aaactactcc taatttacgt ctttttgaga aatatgccgt
     2761 gagagttggc ggtggctata atcatcgctt taatttatca gatgctatca tgctaaaaga
     2821 caatcacatt gcggcagtag gtagtgttca aaaggcaatt gctcaagcgc gtgcctatgc
     2881 tccttttgtg aaaatggtcg aggtggaagt ggaaagcctt gctgctgccg aagaagctgc
     2941 ggcggcgggt gctgatatta tcatgttgga taatatgtca ttggaacaga ttgaacaggc
     3001 cattacccta attgcaggac gttctcggat tgaatgttct ggaaatattg atatgaccac
     3061 tattagccgt tttcgtggtt tagcgattga ttacgtctcc agtggtagtt taacccatag
     3121 tgctaagagt cttgattttt ccatgaaggg tttaacctac cttgatgtct aagttgtaaa
     3181 ataaactaac tttttaaagg atgtctttcc tctagaacga gttttatgtc agatagttta
     3241 aacgcctctt caaatatagt aaaatgaacc aaaaatagta cacaatgtgg tataatcttc
     3301 ttatggcata ttcaatagat tttcgtaaaa aagttctctc ttattgtgag cgaac
//
LOCUS       DAVGJJ010000116         3352 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000116 DAVGJJ010000000
VERSION     DAVGJJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.116"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            11..3130
                     /gene="phtE"
                     /locus_tag="VVE35_001908"
     CDS             11..3130
                     /gene="phtE"
                     /locus_tag="VVE35_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU3062036.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1250 a          546 c          647 g          909 t
ORIGIN      
        1 tgagggattt atgaaattta gtaaaaaata tatagcagct ggatcagctg ttatcgtatc
       61 cttgagtcta tgtgcctatg cactaaacca gcatcgttcg caggaaaata aggacaataa
      121 tcgtgtctct tatgtggatg gcagccagtc aagtcagaaa agtgaaaact tgacaccaga
      181 ccaggttagc cagaaagaag gaattcaggc tgagcaaatt gtaatcaaaa ttacagatca
      241 gggctatgta acgtcacacg gtgaccacta tcattactat aatgggaaag ttccttatga
      301 tgccctcttt agtgaagaac tcttgatgaa ggatccaaac tatcaactta aagacgctga
      361 tattgtcaat gaagtcaagg gtggttatat catcaaggtc gatggaaaat attatgtcta
      421 cctgaaagat gcagctcatg ctgataatgt tcgaactaaa gatgaaatca atcgtcaaaa
      481 acaagaacat gtcaaagata atgagaaggt taactctaat gttgctgtag caaggtctca
      541 gggacgatat acgacaaatg atggttatgt ctttaatcca gctgatatta tcgaagatac
      601 gggtaatgct tatatcgttc ctcatggagg tcactatcac tacattccca aaagcgattt
      661 atctgctagt gaattagcag cagctaaagc acatctggct ggaaaaaata tgcaaccgag
      721 tcagttaagc tattcttcaa cagctagtga caataacacg caatctgtag caaaaggatc
      781 aactagcaag ccagcaaata aatctgaaaa tctccagagt cttttgaagg aactctatga
      841 ttcacctagc gcccaacgtt acagtgaatc agatggcctg gtctttgacc ctgctaagat
      901 tatcagtcgt acaccaaatg gagttgcgat tccgcatggc gaccattacc actttattcc
      961 ttacagcaag ctttctgcct tagaagaaaa gattgccaga atggtgccta tcagtggaac
     1021 tggttctaca gtttctacaa atgcaaaacc taatgaagta gtgtctagtc taggcagtct
     1081 ttcaagcaat ccttcttctt taacgacaag taaggagctc tcttcagcat ctgatggtta
     1141 tatttttaat ccaaaagata tcgttgaaga aacggctaca gcttatattg taagacatgg
     1201 tgatcatttc cattacattc caaaatcaaa tcaaattggg caaccgactc ttccaaacaa
     1261 tagtctagca acaccttctc catctcttcc aatcaatcca ggaacttcac atgagaaaca
     1321 tgaagaagat ggatacggat ttgatgctaa tcgtattatc gctgaagatg aatcaggttt
     1381 tgtcatgagt cacggagacc acaatcatta tttcttcaag aaggacttga cagaagagca
     1441 aattaaggct gcgcaaaaac atttagagga agttaaaact agtcataatg gattagattc
     1501 tttgtcatct catgaacagg attatccaag taatgccaaa gaaatgaaag atttagataa
     1561 aaaaatcgaa gaaaaaattg ctggcattat gaaacaatat ggtgtcaaac gtgaaagtat
     1621 tgtcgtgaat aaagaaaaaa atgcgattat ttatccgcat ggagatcacc atcatgcaga
     1681 tccgattgat gaacataaac cggttggaat tggtcattct cacagtaact atgaactgtt
     1741 taaacccgaa gaaggagttg ctaaaaaaga agggaataaa gtttatactg gagaagaatt
     1801 aacgaatgtt gttaatttgt taaaaaatag tacgtttaat aatcaaaact ttactctagc
     1861 caatggtcaa aaacgcgttt cttttagttt tccgcctgaa ttggagaaaa aattaggtat
     1921 caatatgcta gtaaaattaa taacaccaga tggaaaagta ttggagaaag tatctggtaa
     1981 agtatttgga gaaggagtag ggaatattgc aaactttgaa ttagatcaac cttatttacc
     2041 aggacaaaca tttaagtata ctatcgcttc aaaagattat ccagaagtaa gttatgatgg
     2101 tacatttaca gttccaacct ctttagctta caaaatggcc agtcaaacga ttttctatcc
     2161 tttccatgca ggggatactt atttaagagt gaaccctcaa tttgcagtgc ctaaaggaac
     2221 tgatgcttta gtcagagtgt ttgatgaatt tcatggaaat gcttatttag aaaataacta
     2281 taaagttggt gaaatcaaat taccgattcc gaaattaaac caaggaacaa ccagaacggc
     2341 cggaaataaa attcctgtaa ccttcatggc aaatgcttat ttggacaatc aatcgactta
     2401 tattgtggaa gtacctatct tggaaaaaga aaatcaaact gataaaccaa gtattctacc
     2461 acaatttaaa aggaataaag cacaagaaaa ctcaaaactt gatgaaaagg tagaagaacc
     2521 aaagactagt gagaaggtag aaaaagaaaa actttctgaa actgggaata gtactagtaa
     2581 ttcaacgtta gaagaagttc ctacagtgga tcctgtacaa gaaaaagtag caaaatttgc
     2641 tgaaagttat gggatgaagc tagaaaatgt cttgtttaat atggacggaa caattgaatt
     2701 atatttacca tcgggagaag tcattaaaaa gaatatggca gattttacag gagaagcacc
     2761 tcaaggaaat ggtgaaaata aaccatctga aaatggaaaa gtatctactg gaacagttga
     2821 gaaccaacca acagaaaata aaccagcaga ttctttacca gaggcaccaa acgaaaaacc
     2881 tgtaaaacca gaaaactcaa cggataatgg aatgttgaat ccagaaggga atgtggggag
     2941 tgaccctatg ttagatccag cattagagga agctccagca gtagatcctg tacaagaaaa
     3001 attagaaaaa tttacagcta gttacggatt aggcttagat agtgttatat tcaatatgga
     3061 tggaacgatt gaattaagat tgccaagtgg agaagtgata aaaaagaatt tatctgatct
     3121 catagcgtaa ggaatagcag tagaaaaagt ctgaatcaaa aatgaagttc tctcaaaagt
     3181 tagaaataaa actctgactt tgggagaatt tcattttatt attaatatat aaaatttctt
     3241 gacatacaac ttaaaaagag gtggaatatt tactagttaa ttaattggtt aattaatttc
     3301 tataatcatt taataatgaa gacatcatat aaataattat agaaatgagg ta
//
LOCUS       DAVGJJ010000117         3282 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000117 DAVGJJ010000000
VERSION     DAVGJJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.117"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>3282
                     /locus_tag="VVE35_001909"
     CDS             <1..>3282
                     /locus_tag="VVE35_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEU3062037.1"
                     /translation="EETTVNGTHVYKVTAIADNLVSRTADNKFEEEYVHYIEKPKVHE
                     DNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTGKLLSSEGKQFA
                     ITELEHPLFDKIKAGTVKNINFENIDINVSNQDNIATIANEINGTTRIENIKATGTIT
                     ARNNVAGFVNNIKENAALENVAFIGNINSVGNNSTVGGIAGSNYMGFVNKAYVDANIT
                     AANASASMLVPLVTYMLNSYQYGTKSKVTNSVAKGTLDVKNTRFVGGVVAKTWPWGVI
                     KDIVTYAKVIKGEEVYGSNEINDGDARQFLSDIYRVNGASSGANNFTNLQKIKQLTKE
                     QADAKVATFNITADKLESELSPLAKLNEEKAYSSIQDYNAEYNQAYKNLEKLIPFYNK
                     DYIVYQGNKLNKEHPLNTKEVLSVTAMNNNEFITNLDEANKIIVHYADGTKDYFNLSS
                     SSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILEPVDLQSQTMYQHLNRLG
                     DYRVNAIKDLYLEESFTAVKKNLTNLITKLVQNEEHQLNDSPAARQMLRDKVEKNKAA
                     LLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNIKGSRTF
                     DAFGEVLAKYTKSGNLDVFLNYNRQLFTNIDNMNDWFIDATKDHVYIAERASEVEEIK
                     NAKYRAYANLNVAQHRKMILPLLNITKSHMFIASNYNAIAIGSAEKLGKKSLEDIKAS
                     VDYAADGYRNYYDFWYRLVPDNVKQYLLRDTVIPIWEGYNAPGGWVEKYGRYNTDNVY
                     TPLREFFGPIEKYYGYNGTGAYAALYDNPMDPRSDVSYVALNMIGDYGISVYTHETTH
                     INDRKIYLGGFGRREGTDAEAFAQGMLQLPVPGSGFNEYGSLGLNTVFKKPNDGNQWY
                     DTDPKSLTTRDDIDKYMRGYNDALMLVDHLEADAVINQNNKELNGAWFKKIDKKFRDA
                     SQLNQWDEIRNLTTEEKDNLNITSIDSLIDQQLMTNRQIGNRVYKPEVVSQYDQSPYV
                     GVEMTAGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYLNGFLGYTSNKYKQQSTKDGES
                     VLSDEY"
BASE COUNT         1238 a          500 c          577 g          967 t
ORIGIN      
        1 gaagaaacta cggttaacgg aacacatgta tataaagtta cagcaatcgc agacaattta
       61 gtctctagaa ctgctgataa taaatttgaa gaagaatacg ttcactatat tgaaaaacct
      121 aaagtccacg aagataatgt atattataat ttcaaagaat tagtggaagc tattcaaaac
      181 gatccttcaa aagaatatcg tctgggacaa tcaatgagcg ctagaaatgt tgttcctaat
      241 ggaaaatcat atatcactaa agaattcaca ggaaaacttt taagttctga aggaaaacaa
      301 tttgctatta ctgaattgga acatccatta tttgacaaga ttaaagctgg aactgttaaa
      361 aatataaatt ttgaaaatat tgatattaat gtgtctaatc aagataatat cgcgacaatt
      421 gcgaatgaaa tcaacggaac cacacgtata gaaaacatca aagcaactgg aacgataaca
      481 gccagaaata acgttgctgg atttgtaaac aatatcaaag aaaatgctgc tctagaaaac
      541 gtagcattta taggtaatat caattctgtt ggtaataatt ctacagtcgg aggaattgct
      601 ggttcgaact acatgggatt tgttaataaa gcttatgttg atgctaacat tactgccgct
      661 aacgcaagcg caagtatgct agttcctttg gttacatata tgttaaatag ctatcagtat
      721 ggaactaagt caaaagttac caattcggtt gctaaaggaa ctttagatgt taaaaatact
      781 cgttttgttg gtggtgttgt agctaaaact tggccttggg gagttataaa agatattgtt
      841 acctatgcta aagtaattaa aggtgaagaa gtatatggtt caaacgaaat taatgacgga
      901 gatgcacgcc aattcttatc tgatatttat agagtcaacg gcgctagcag tggagctaat
      961 aattttacca atctgcaaaa aataaaacaa ttaactaaag aacaagctga tgctaaagtt
     1021 gctactttca atattactgc tgataaatta gaaagtgagc tatctcctct tgcaaaactt
     1081 aatgaagaaa aagcttattc tagtattcaa gattacaacg ctgaatataa tcaagcctat
     1141 aaaaaccttg aaaaattaat accattctac aataaagatt atattgtata ccaaggtaat
     1201 aaattaaata aagaacaccc tctaaatact aaagaagttc tttctgttac agcgatgaac
     1261 aacaatgagt ttatcacaaa cctagatgaa gctaataaaa ttattgttca ctatgcagac
     1321 ggtacaaaag attactttaa cttatcttct agcagtgaag gtttaagtaa tgtaaaagaa
     1381 tatactataa ctgacctagg aattaaatat acacccaata tcgtgcaaaa agataacact
     1441 actcttgtta atgacataaa atctatttta gaaccagtag atcttcaatc tcaaacaatg
     1501 tatcaacacc taaatcgatt aggtgactat agagttaatg caatcaaaga tttatattta
     1561 gaagaaagct tcacagccgt taaaaagaac ttaacaaacc taatcacaaa attagttcaa
     1621 aatgaagaac accagttaaa tgattctcca gctgctcgtc aaatgcttcg tgataaagtc
     1681 gagaaaaaca aagctgccct attattaggt ttaacatact taaaccgtta ctacggtgtt
     1741 aaattcggtg atgttaatat taaagaatta atgttattca aaccagactt ctatggtgaa
     1801 aaagttagcg tattagacag attaattgaa atcggctcta aagagaacaa cattaaaggt
     1861 tcacgtacat tcgacgcatt cggtgaagta ttagctaaat atactaaatc aggtaattta
     1921 gatgtattct taaattataa tagacaatta ttcacaaata tagataatat gaatgattgg
     1981 tttattgatg ctacaaaaga ccatgtctac atcgcagaac gcgcttctga ggtcgaagaa
     2041 attaaaaatg ctaaatatag agcatatgct aacttaaacg tagctcagca cagaaaaatg
     2101 attttaccat tattgaatat aactaaatca cacatgttta ttgcctctaa ctacaacgca
     2161 attgcaattg gtagtgctga gaaattagga aaaaaatcat tagaagacat taaagcatct
     2221 gttgattatg ctgctgatgg gtacagaaac tactatgatt tttggtatcg tctagttcca
     2281 gataatgtta aacaatatct actaagagat actgtgattc ctatctggga aggttataat
     2341 gcgccaggtg gctgggttga aaaatacggt cgatataata ctgacaatgt atatactcct
     2401 cttagagaat tctttggacc tatagaaaaa tattatggtt ataatggaac aggagcatat
     2461 gctgcacttt atgataatcc aatggatcct cgttctgatg taagttatgt tgctctaaat
     2521 atgattgggg attatggtat ttctgtatat actcatgaaa caacacatat aaacgatcgt
     2581 aaaatctatt tgggtggttt tggacgtcgt gaaggtactg atgctgaagc atttgctcaa
     2641 ggtatgttac aactaccagt cccaggaagt ggatttaacg aatatggttc attaggtctg
     2701 aataccgtat tcaaaaaacc taatgatggt aatcaatggt acgatacaga tcctaaatct
     2761 cttacaacac gtgatgatat cgataaatat atgcgtggtt acaatgatgc tttaatgctt
     2821 gtcgatcatt tagaggcaga tgctgttatt aatcaaaata ataaagagtt aaatggagca
     2881 tggttcaaga aaatagataa gaaattccga gatgccagcc aattaaatca gtgggacgaa
     2941 attcgaaatc taacaactga agaaaaagat aacttaaata ttacatcaat tgatagttta
     3001 atagatcaac agttaatgac taatagacaa attggaaacc gtgtttataa acctgaagtc
     3061 gttagccaat acgatcaatc accttatgtg ggggttgaaa tgacagccgg tatctacgga
     3121 ggtaatacta gtaaaggtgc tcctggagct gtttcattca aacataacgc ctttagatta
     3181 tggggttact acggatacct taacggattc ctaggctata cttcgaataa atataaacaa
     3241 caatctacaa aagatggcga gtcagttcta agtgatgaat at
//
LOCUS       DAVGJJ010000118         3230 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000118 DAVGJJ010000000
VERSION     DAVGJJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.118"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(134..388)
                     /locus_tag="VVE35_001910"
     CDS             complement(134..388)
                     /locus_tag="VVE35_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU3062038.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(408..2249)
                     /gene="typA"
                     /locus_tag="VVE35_001911"
     CDS             complement(408..2249)
                     /gene="typA"
                     /locus_tag="VVE35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU3062039.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2411..3136
                     /locus_tag="VVE35_001912"
     CDS             2411..3136
                     /locus_tag="VVE35_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU3062040.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          954 a          715 c          615 g          946 t
ORIGIN      
        1 aatagatgcc attaacgaat tttattcaag tttttcccat tcatactata caagcaaaag
       61 agatggtgtt aactaaaaag caattcaaac tattataaaa ttcctagcaa aaagagagcc
      121 gaaactctct tttttatctt cttttacttt ttttgactgg catgagtgtg atgtctctca
      181 cactaaagta agctaggatc aacatggcta ttgctaggaa tatttctgtt ggtaattgaa
      241 aaattttcag aaaagataga accaataaaa tcaagagtgc cactaaaata cataccatag
      301 cgacgatatt gactgtccct ttaatgcttt ctggtgtcgc aaatacatag agtaggagca
      361 gtaaaattcc taggactaaa tagaccatct ttctctcttt ctagctctta ttcagctgat
      421 tttttcttct tgttagcttt ctcacgctct gctttgttaa ggatttgttt acgcaaacgg
      481 atagactcag gcgttacttc catgtactca tcgtcgttca agaactcaag agactcttca
      541 agtgtcaaga tacgaggcgt cttgataaca gctgtttggt ccttagtagc tgaacgaacg
      601 ttggtcattt gttttgcctt cgtgatgtta actgtcaagt cattttcacg agagttttcg
      661 ccgatgatca ttccttcata aacctcagta cctgggttga caaagatcgt accacgttct
      721 tcgatagaca tgattgagta agttgtagcc ttaccagcat cgatagaaac aagggcacca
      781 cggtgacgtc caccaatttc ccctggaatc aatggcaagt actggtcgaa ggtatggttc
      841 atgataccgt aaccacgagt cattgacaag aactcagttg agtatccaat caaaccacgc
      901 gctggaacaa ggaagaccaa acgagtttga ccattaccag ttgaaatcat atccaacatt
      961 tcacctttac gttcagaaag gctttggata acagaccctt ggtattcttc tggagtgtcg
     1021 atttgtacac gttcaaatgg ttcacattta acaccgtcga tttcttttac gataacttct
     1081 ggacgagata cttgaagttc atagccctca cgacgcattg tttcgataag gattgacaag
     1141 tgcaattctc cacgtcctga aacagtccat ttatctggtg aatcagttgg gtcaacacga
     1201 agggaaacgt ctgtttgcaa ttctgcctgc aagcgttctt ccaccttacg agaagttacc
     1261 catttacctt ctttaccagc aaatggtgag ttgttgacca agaaagtcat ttgaagagtt
     1321 ggctcatcga tgtgtaggat tggaagagct tctactgcat ctgtcggagt gatggtttca
     1381 ccgacaaaga tgtcttccat acctgaaacg gcaatcaagt cacccgcttt ggcttcttgg
     1441 atttcacgac gttccaaacc aaagaaaccg aagagttttg taacacggaa gtttttagtt
     1501 gtaccgtcaa gtttagaaag ggtaacctgg tccccaacct taactgtacc acggaagaca
     1561 cgaccgatac cgatacgtcc aacaaagtca ttgtagtcca aaagtgacac ttggaactgc
     1621 aaaggctcat ctgagttatc tactggagct gggatatggt cgataatcgt gtcaaagatt
     1681 ggtgccatag tcgcttcttg gtcagctgga tcatctgaca atgaagaagt tccgttgatc
     1741 gctgaagcat aaaccactgg gaaatcaagc tggtcgtcat ctgcaccaag ctcgatgaaa
     1801 agttccaaga cttcatccac tacttctgct ggacgagctg atggcttatc gattttgtta
     1861 acaaccacga ttgggacaag atcttgttcc aaggcttttt tcaatacgaa acgagtttgt
     1921 ggcatggttc cttcataggc atctacgacc aagacaacac cgtcaaccat tttcatgata
     1981 cgctcaactt ctccaccaaa gtccgcgtgt cctggtgtgt ccataatgtt gatacgagtt
     2041 ccgttgtaag caacggcagt gtttttagca aggatggtaa tcccacgctc tttttcgata
     2101 tcgtttgagt ccatagcacg ctctgccaat tcagtacgtg catcaagcgt ttctgattgt
     2161 ttcaataatt cgtcaaccag ggttgtttta ccgtggtcaa cgtgggcgat aatcgcaatg
     2221 ttacggatat cttctcttaa ttttgtcatg atttcctcta taatattcaa aatttatttt
     2281 ctaactgaac gattatacca taatttcaaa taaataacat aactcaagca agtgtaaatg
     2341 ttttcactcc gcttttcttt tcacgtcaag ccttttcaaa gcaagcgact tatgataaga
     2401 taggcacagt atgcgtttag ataatttatt agcccaagaa aaagttagcc gaaaggccat
     2461 gaagcaagca ctcctcagag gggaaattct agtcgatggt tgcccagccc gctccctagc
     2521 tcaaaatatc gatacaggac tacaagaact cctttttcag gatcgaatca ttcaaggcta
     2581 tgaacacacc tatcttatgc ttcataaacc tgctggtgtc gttacagcca acaaagacaa
     2641 ggaacttccg accgtcatgg acttgcttcc atctgacatc cagtctgaca agctctatgc
     2701 cgtcggccga ctggaccgag atacaacagg actccttctc ttgaccgata acggtccctt
     2761 gggcttccaa ctcctccatc cccaatatca tgtcgataag acttatcaag ttgaggttaa
     2821 tggacttcta acacctgacc atatccaaac ctttcaaaaa ggaattgtct ttttagatgg
     2881 cactatctgt aaacctgcaa gactagagat tctatcagca agtccttccc tcagtcaagc
     2941 ctctatcacc atttcagaag gaaaatttca tcaaatcaag aaaatgttcc tctcggttgg
     3001 tgttaaggtg actagcctca agagaatcca atttggggac ttcacattga acccagattt
     3061 agcagaaggt aactaccgcc ctttgaacca aaaagagtta caaatcatta aaaactattt
     3121 agagatgagt cgataaaaca aaaaaagctt taaaactaaa gcttttttct tttatttatc
     3181 gaaaaattaa ggcgattgct acaatccagt taactacaga aatcacaatt
//
LOCUS       DAVGJJ010000119         3085 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000119 DAVGJJ010000000
VERSION     DAVGJJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.119"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..927
                     /locus_tag="VVE35_001913"
                     /pseudo
     CDS             1..927
                     /locus_tag="VVE35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1317..2180
                     /locus_tag="VVE35_001914"
     CDS             1317..2180
                     /locus_tag="VVE35_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU3062041.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYVPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            2646..>3085
                     /locus_tag="VVE35_001915"
     CDS             2646..>3085
                     /locus_tag="VVE35_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3062042.1"
                     /translation="MDRLQTMLNKIQVDTYHKNGWLFVKYSNNKLTQGWKLHVSSQLK
                     DACNIFYIVAQELEKERCNYKVLDCLDELKKLNSPREVSPTANKFITIYPSSRKQAKR
                     IILNLKEKLEKYKAPRILSDFQCGEYSPIHYRYGAFKAIKNYNFS"
BASE COUNT         1096 a          453 c          557 g          979 t
ORIGIN      
        1 gacagattat gatgagaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagattctg agggtaaaac ctaatggaaa gattatttta aagtcaacag ttccgatcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc ttttttaatg aactagattc ttttgctgag
      421 aggagaagtt taaatgctga agttgtaata aaaggtgttt gtttagatcc aagaattgga
      481 aatttttata ataatccttc ttttggattt ggaggatatt gtcttcccaa agatactaaa
      541 caattaaaaa aagaatttat agaaataaat gccccagtga tagaagcgat tgatatttca
      601 aacacaaata gaaaacagtt catagttaaa caaatattgg aacgaaagcc aaagatagta
      661 ggaatatata aattggggat gaaatataat tcagataatt acaaagagtc agctatttta
      721 agtataatta atgaactatt gattgtcggt ataaaaattt tagtatatga accaaattta
      781 aacgttagta tagataacgt aatttttgaa aagaattttg aactatttac aaaacaaagt
      841 gatctaattg ttgcaaatag atgggatagg ggccttgaag cttataaaga taaagtttac
      901 acaaggggga tctggataag agattaacta ttgatattaa aaaggcaata atttataaaa
      961 tttgtacaaa aaaatgttaa attttcacat tttcatagta tactgtttaa tagaaagtag
     1021 gttctttgta accgctccat tataaaatcc tttcactctt ccggtatact attaggaaaa
     1081 acataactgt attttgagct cacacctcat cgttccagtg gctgttcccc aagctcgtgt
     1141 actgcccaaa aaagttagac attctattta gaacgaggat tgagttctgt attgtacaag
     1201 gctcggtcct tttagagtca gcttaaggct ggcttttttg ccctcttaaa tatcagaata
     1261 ttttgacaaa tgattgtaaa taatgatata atatgcaaaa gctaggaggt ggtcagatga
     1321 ttgagagaat ggaattggga gaattttaca aggaattgcg cttggcgaga aaacttaagc
     1381 agtcagatgt ggcctgtgag ggtctaacag cttctcagtt gtccaagttt gaactaggac
     1441 agtctatgtt atctgcagat aagctcatat tagccatcca agggatcaat atgaattttg
     1501 atgagtttgg gcacaagctc aacaactatc aagaatctcc acatatgcga atcggtcgaa
     1561 aggttgtgga tcgctttgcc catcaggata tagctgcttt agagcagcta ttggaggaag
     1621 tcgatcaaga acagatggcg cagacctatc gtcgtttgaa tgccattgtg attaaaaatg
     1681 cactccattc cttagataaa agctacccgc taacagagga ggatagcgag tttttgacta
     1741 cctacctcta cgctattgag tcttggacct ggtttgaact ctatcttttt tgcaatacta
     1801 tgcccttctt gagcaatcaa gatctgatct ttttatcaac ctccttactc gaaaaatcca
     1861 aggaatttaa agagttagta cacaatcgat ggtatatgaa aagtggttat cttaatatca
     1921 tctcagaact tatggagcgc aagctatttt cttatgttcc aatctttgaa gctgagctgg
     1981 agcgcatgct ccgtccatac gatgtttttg aaaaagtatt gtggcagttt ttaaagaaaa
     2041 tgcagatttt tcttcaaacc aaaggaagca atcaaaaaga gattgaacac tttatccaat
     2101 ctctgcaagt attagaaaat ccacaattga cagccctttt tgaattgcgt ttgcagcaat
     2161 acaaagagct tatcgattag ttagaaatgg gaaaaagata ataatatcag caacgatcgt
     2221 caataatgtt aaaacttgtt tcttcatatt acttccctcc tcattagtaa taactttatt
     2281 ataccaggga aataatcaaa tctaccaaaa tcgcaaatct gaaatttcta taagaaaaaa
     2341 tatcaaatat gcgattttct aaaataagcc aatttccgtg ttatactgtc cttgtaaaac
     2401 atttgaagca aatcctaggt cgcagaagtg gtttacaaaa aaataatcaa aggagaaaag
     2461 aaatgcataa tctaatggaa cttgaattgt tacctgaaga gccagtaatt actgtatcac
     2521 gggaagtttg taagtggact tgctcattta ctgctcttta agaataactg gttaaaatta
     2581 aggatagttg attactaatt aactatcctt aatttcattt cagagataat gtatttatta
     2641 tatttatgga tagattacaa acaatgctga ataaaataca agttgatact taccataaaa
     2701 atggatggtt atttgtgaag tatagtaaca ataaacttac acagggatgg aaacttcatg
     2761 taagcagtca actaaaagac gcttgtaata ttttttatat tgttgcacaa gagctggaaa
     2821 aggaaagatg taactacaaa gttctagatt gtttggatga gttaaaaaaa ttaaattctc
     2881 ctagggaagt ttctcctact gcaaataagt ttatcactat atatccgtct agtaggaagc
     2941 aggccaaacg aataatatta aatctaaaag aaaagttaga aaaatataaa gctccaagga
     3001 ttttatcaga ttttcaatgt ggtgagtatt ctccaataca ttatagatat ggtgcgttta
     3061 aagcaattaa aaattacaat ttttc
//
LOCUS       DAVGJJ010000120         2978 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000120 DAVGJJ010000000
VERSION     DAVGJJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.121"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVE35_001916"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VVE35_001916"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU3062043.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIIDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1400..2176
                     /gene="recX"
                     /locus_tag="VVE35_001917"
     CDS             1400..2176
                     /gene="recX"
                     /locus_tag="VVE35_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU3062044.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2265..2798
                     /locus_tag="VVE35_001918"
     CDS             2265..2798
                     /locus_tag="VVE35_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU3062045.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          959 a          538 c          535 g          946 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaataatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggtcttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctcgag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtgataa gtaagtaatt ctgctaagcg
      841 attagcaatc acttgggttt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atacccctat cttatcacat
     1381 tttgagttat aatagagcta tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct
     1441 gatggagctt gataatggcg acaaatgcta tatcaccgaa gatacaattg ttcgttttat
     1501 gttatcgaga gataaggtga taagcgaaga ggaattgaaa gagattcaag actttgctca
     1561 attttcttat ggtaagaatc tggccctcta ccacttatcc tttaaagcac gcactgaaaa
     1621 agaagtcaga gaatatctga aaaaatacga tattgataaa aacatcgttt ctcaagttat
     1681 tgctaatctt aaagaagata agtggattaa tgatggtcag tacgcttatg ctatcatcaa
     1741 taccaatcaa ctttcaggag acaagggtcc ttatgtgctg actcagaaac tagctcaaaa
     1801 agggatttca aaatctacta tagaagagaa cttgaaagaa tttgattttt ctgaagttgc
     1861 tcaacgtgta gctaataaac tattgaaaaa atatgaggga aaacttccag ctcgtgcctt
     1921 gcaagataag attatccaga acttgactaa caagggcttc tcttattctg atgctaaaat
     1981 tgcctttgac gacttagata gccaagttga ccaagaaacg actcaagaac tcatcttcaa
     2041 ggaacttgat aagcaatata ctaagtatgc tcgaaagtat gaaggatacg aactgaagca
     2101 gcgtttaact caagttttag cacgaaaggg ctacgatttt tcggatatag caagcgctct
     2161 tagagaatat ctttaatatt tccaagtgaa attcacagat tttaggtaat tttatggtac
     2221 aatagtaaat gataaactta taaattgtag aaagttggtt agttatgaag cttccaaaag
     2281 aaggcgactt tattacaatt caaagttata agcatgatgg gagtctccac cgaacttggc
     2341 gggacaccat ggtactaaaa acaacagaaa acgccattat tggtgtcaac gatcatacac
     2401 ttgttaccga aagtgatggt cgtcgttggg tcactcgaga accggctatt gtttactttc
     2461 acaagaaata ttggtttaat atcattgcca tgattcgtga taatggaact tcttactatt
     2521 gcaatatggc tagcccctac tatctggatg aagaagcact gaagtatatt gattacgatt
     2581 tggatgttaa aatttttaca gatggggaaa aacgtctctt ggacgttgaa gagtacgagc
     2641 gtcacaaacg caaaatgaat tattctgatg acttggacta tattttaaaa gaacatgtca
     2701 aaattcttgt tgattggatt aacaatggac gtggtccttt ctcagaagcc tatgtaaaca
     2761 tttggtacaa gcgctatgta gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa
     2821 aatcctgacc tttttttggt agtataaaat aaattgtgta aacacaaaaa ggaataaatc
     2881 cattatagca gagttacaac acattactag aaagagattt atgcagaatt cgtaaaatat
     2941 ataaaacaaa tagatatact gttatttttt aatgtctt
//
LOCUS       DAVGJJ010000121         2939 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000121 DAVGJJ010000000
VERSION     DAVGJJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.122"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            175..1914
                     /locus_tag="VVE35_001919"
     CDS             175..1914
                     /locus_tag="VVE35_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU3062046.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWAANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            2189..2491
                     /locus_tag="VVE35_001920"
     CDS             2189..2491
                     /locus_tag="VVE35_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062047.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSRNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            2547..>2939
                     /locus_tag="VVE35_001921"
     CDS             2547..>2939
                     /locus_tag="VVE35_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3062048.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQE"
BASE COUNT          982 a          443 c          621 g          893 t
ORIGIN      
        1 tctaatcaat agtagtaatt caaagccttt tttaaggctt tttgtatact ttaaaagata
       61 gttcctttaa caacggacat tccttgcaaa tagtttacaa aaatagtata ctggattcat
      121 tgagtttgaa aacgtttgcg taaaatttga atgaatactt taggagacaa attgatggaa
      181 ttgagtgcta tttaccatag gcctgagtcg gagtatgcct atctttataa ggataagaaa
      241 ctccatattc gaattcgaac taagaaaggg gacattgaaa gcatcaactt gcactatggg
      301 gaccctttta tctttatgga ggagttttat caggatacaa aagaaatggt caagataact
      361 tctggtacct tatttgacca ttggcaggtt gaagtgtcag ttgactttgc acgtatccag
      421 tatctctttg agctcagaga tacagaaggt caaaatattt tgtatggcga taaagggtgt
      481 gtggaaaatt ctctagaaaa tcttcatgca attgggaatg gatttaagtt gccttatctt
      541 catgagattg atgcctgcaa ggttcctgac tgggtttcaa atacggtatg gtatcagata
      601 tttcctgaaa gatttgccaa tggcaatgct ctattaaacc cagaagggac tttagactgg
      661 gattcatctg tcacacctaa gagcgatgat ttctttggtg gtgatttaca ggggattatt
      721 gatcatatgg attacttgca agacttgggt attactggac tatatctttg tcccatcttt
      781 gaatctacaa gcaatcacaa gtacaatacg acagattact ttgaaattga ccgtcatttt
      841 ggagacaagg agacctttcg ggaactggtg gatcaagcgc atcatcgtgg catgaaagtc
      901 atgctggatg cggtatttaa tcatattggt tcgcaatctc ttcaatggaa aaatgtcgtc
      961 aaaaatggtg aacagtctgc ttataaggat tggttccata ttcaacaatt cccagtgaca
     1021 actgaaaagc tagttaataa gagagactta ccctatcatg tttttggttt cgaggactat
     1081 atgcctaagc taaatacagc caatccagag gtcaagaatt atcttttaaa ggttgcgact
     1141 tattggattg aagagtttaa tatcgatgct tggcgtttgg atgtggctaa tgagattgac
     1201 catcagttct ggaaggattt tcgtaaggca gttttagcta aaaatcctga tctttatatc
     1261 ctaggagaag tctggcatac atctcagcct tggctaaatg gagatgagtt ccatgccgtc
     1321 atgaattatc ctttatctga tagtatcaag gactatttct tacgaggaat taagaagaca
     1381 gaccagttca tcgatgaaat caatggagag tctatgtatt acaagcagca gatttcagag
     1441 gtcatgttta atctcttgga ttcacatgat acagagcgaa tcctgtgggc ggccaatgaa
     1501 gatgttcaac tggttaaatc agccttagcc tttctctttt tacaaaaagg aacaccgtgc
     1561 atttattacg gaaccgagct agccttgact ggaggaccag atccagattg tcgtcgttgt
     1621 atgccttggg aacgtgtatc aagtgacaat gatatgctga actttatgaa gaggctgatt
     1681 aaaattcgga aatacgcgtc agtaatcatt tcgcatggca agtatagcct tcaagaaatc
     1741 aaatctgatc tagtagctct ggaatggaaa tacgaaggac ggatcctcaa agcaatattc
     1801 aaccaatcaa cagaagatta tcttttagag aaagaagcag tagcactagc aagcaattgc
     1861 caagaattgg agaatcagct tgtcatctct ccagatggat ttgtgatttt ctaaaaacta
     1921 gttgatgaag attatggtac atttcatatc ttatatagta taataaggtt agttactaaa
     1981 cttgtaaagg agaacttaaa tgaattgtag aggacatgaa acaagacaaa gaattgttag
     2041 agattttgaa gtttagccta aagcacatat taagctgtta gcaaatcaac aaaaacatag
     2101 tgatgcagga gcaactattg aagatgaata ttatgtattt atcgctgaga gtaaaattga
     2161 tggcaagaag gaagttattc agtgttgcat gggtgcggca agggattttt tagaactaat
     2221 taatcacaaa gggctacctc tttttaatcc gcttgtaggt gattctcatg taaataatag
     2281 acaagaatat gacaatacag ggagtagaaa tttacaacct gaaaagtgga atgaaactgc
     2341 aaagcagctt tataatgcta taatgtggtt gattatttta tggaatgcta agccggatac
     2401 acctttattt aattttaaag acgaagtaat taagtataaa acatatgagc cttttgaaag
     2461 cagtataaaa ttggtatcaa tcaatgagta acaaagtatc agcatgattc gaaacatttg
     2521 cttgtaattt gcggaggaac atcaatatgg atggaataac aaaagatttt ataaaaacgg
     2581 ctaaactaat gaaacaatta tggccccaat tgaccgataa agaagctatt gatgaagtaa
     2641 aaaaatatac gaatggcaaa aatactgcaa tctttactga agttgaaggt gacacaattg
     2701 taggtctagc actatgttca ctcagatttg attatgttga aggttgtaaa tatagtcctg
     2761 ttggattctt agaagggatt attgttgacg aggaatatcg tttaaaggat attgctaaaa
     2821 atctctgtac aaaatgtgag gaatgggcga aaaataaagg atgtaaggaa tttgcaagtg
     2881 actgtacttt aacgaatacg gattctataa gatttcatct caatattgga tttcaggag
//
LOCUS       DAVGJJ010000122         2886 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000122 DAVGJJ010000000
VERSION     DAVGJJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.123"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..956
                     /locus_tag="VVE35_001922"
     CDS             <1..956
                     /locus_tag="VVE35_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU3062049.1"
                     /translation="VTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHDKIPVIEKE
                     KIIEFATHEVVDEVFVNLPGESYDIGEIISRFEKMGMDVTVNLKAFDKNLGRNKQIHE
                     MVGLNVVTFSTNFYKTSHVISKRILDICGATIGLILFAIVSLVLVPLIRKDGGPAIFA
                     QTRIGKNGRHFAFYKFRSMQIDAEAIKEQLMDQNTMQGGMFKIDNDPRVTKIGRFIRK
                     TSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPEQKRRLSFKPGITGLWQVSGRSK
                     ITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
     gene            987..2159
                     /locus_tag="VVE35_001923"
     CDS             987..2159
                     /locus_tag="VVE35_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU3062050.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKTIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQEIGGTLLVNPDGHEWLREKWSLPVRKYWKYSERLMVKYA
                     DLLVCDSKNIEQYIQEDYKQFQPKTTYIAYGTDTTRSTLNSSDEKVRAWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASSSKKDFVLITNVEQNKFYNQLLASTGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDDLSQVIEKVEQFDAKIIDELDRQSNQRIVDFFTWEKIVTDYEKLFKK"
     gene            2156..>2886
                     /locus_tag="VVE35_001924"
     CDS             2156..>2886
                     /locus_tag="VVE35_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3062051.1"
                     /translation="MKILHYTLGFQPQRTGGLVKYAEDLMLEQIAQGYQVAALYPGRI
                     KFFSKKIEIIKATSRQFECYELLNSLPMALFGGISDPTAFLTPCDKNVYRTFLEKVQP
                     DIIHIHSFMGLHKEFLEIAKSLNIRVVFTSHDYYGLAPVPHFYFNGVDYSDKSTNLTW
                     NIMSSNALSVKKLRLFQVSFYPTIRKLLKLLGKNPKSKKNLAIRDVIEEQDYSELRYY
                     YNEMFHLIDGYLFNSRLAKKVYEIN"
BASE COUNT          977 a          413 c          587 g          909 t
ORIGIN      
        1 cagtaacgaa aaatatggaa aaagttcttg ataaattgct agaatctgat gaactttcat
       61 ggaaattggt agcagtaagt gttttggata aatctgattt tcaacatgat aaaatacctg
      121 taattgaaaa ggaaaaaatt attgaatttg caacgcatga agttgtggat gaggtgtttg
      181 tcaatcttcc aggagagagc tacgatattg gagaaattat ctctaggttt gagaaaatgg
      241 ggatggatgt aactgtaaat cttaaagcat ttgataagaa tttgggtcgc aataaacaaa
      301 ttcatgagat ggtaggattg aatgtagtca ctttctctac aaatttttat aaaactagtc
      361 atgtgatttc aaagagaatt ctcgatattt gtggtgccac tattggcctt attctttttg
      421 ctatagttag tctagtttta gttccattga ttcgtaaaga tggcggacca gctatttttg
      481 ctcaaactcg tatagggaaa aatggtcgac attttgcctt ttataaattc cgttcgatgc
      541 agatcgatgc tgaagctatc aaagaacagt tgatggatca aaatacgatg caaggtggta
      601 tgtttaagat agacaatgat cctcgtgtta caaaaattgg tcgctttatt cgtaaaacca
      661 gtttagatga gttaccccag ttttggaatg tctttatagg agatatgagt ttggtgggaa
      721 cacgtccacc tacagtagac gagtatgatc agtatactcc agaacagaaa cgtcgactca
      781 gctttaaacc tggtattaca ggtttatggc aggttagcgg ccgtagtaaa ataaccgatt
      841 ttgacgatgt tgtaaaatta gatgtggctt atattgataa ttggacaatc tggaaagata
      901 ttgaaatttt gcttaaaact gttaaagttg tatttatgag agatggagcg aagtaatttc
      961 tgtatatcca tcatattagg agagaaatga aaaagtcagt ttatatcatt ggttcaaaag
     1021 gaatccctgc taagtatgga ggatttgaaa cttttgttga aaaattaaca gccttccaac
     1081 aagataagac tatccaatat tatgtggctt gtatgcgtga aaactctgca aaatcaggga
     1141 ctactgagga tgtttttgaa cataatggtg ctatctgtta taacgtcgat gttcctaata
     1201 ttggtccagc gcgagccata gtatatgata tcgcggcaat taacagagct attaaaattg
     1261 ccaaagaaaa taaggatgaa gatccaatct tctatattct agcttgtcga atcggtccgt
     1321 tcatccatgg aattaagaaa aaaatccagg agataggtgg gactcttcta gttaatccag
     1381 atggtcatga gtggctacga gaaaaatgga gtttacctgt tcgcaaatat tggaaatact
     1441 ctgaaagact tatggtcaaa tatgcagatt tattggtttg tgacagtaaa aatatcgaac
     1501 agtatatcca agaagattat aaacagttcc aacctaagac aacctatatc gcttatggga
     1561 cagatacaac acgctccaca ttaaatagca gtgatgaaaa agtgcgtgct tggttcaagg
     1621 agaaaaatgt ttctgaaaac gagtattacc tagttgtagg acgttttgtt ccagaaaata
     1681 attatgagtc tatgatacgt ggtttcttgg cgtctagctc taagaaggat tttgtcctaa
     1741 tcacaaatgt agaacagaat aaattttata atcagctatt agcaagtact ggatttgata
     1801 aagatccacg agtgaaattt gttggaacag tctacgacca agaacttctg aagtatattc
     1861 gagaaaatgc ttttgcttac ttccacggac atgaagttgg tggaacaaat ccttcgttac
     1921 ttgaagcatt agcatccaca aagttaaact tactactcga tgttggtttt aaccgcgaag
     1981 ttggggagca aagtgcgatc tattggaaaa aagatgatct ttcccaagta atcgagaaag
     2041 ttgaacaatt tgatgcaaaa attattgatg agttagatag acaatcaaat cagagaattg
     2101 tggatttttt cacttgggaa aagattgtca cagactacga gaaattattt aaaaaatgaa
     2161 aatattacat tatactttag gatttcaacc ccagcgaaca ggtggtttgg ttaaatatgc
     2221 tgaggattta atgctagagc aaattgctca aggttatcaa gtggctgccc tatatccagg
     2281 aagaattaag ttcttttcaa aaaaaataga aataattaaa gcgacgtcaa gacagtttga
     2341 gtgttatgag ctgcttaata gcttacctat ggctttattt ggagggatat ccgatccaac
     2401 tgctttcctg acaccttgtg acaaaaatgt ttatcgtact tttttagaaa aagtacaacc
     2461 cgacattata catattcact cgtttatggg attgcataaa gaattcctcg aaattgctaa
     2521 aagcttgaat attagagtgg tttttacgag ccatgattat tatggattag cacccgtacc
     2581 tcatttctat tttaatgggg tggattatag tgataaaagt acaaacttaa catggaatat
     2641 tatgtcttcc aatgctttaa gtgtgaaaaa actaagactt tttcaggttt cattttatcc
     2701 tactattcgt aaacttttga aactattagg gaaaaatcca aaatctaaaa agaatttagc
     2761 tattcgagat gttattgagg agcaagatta tagtgagctt cgatactatt ataatgagat
     2821 gtttcactta atagatggtt atttgtttaa tagtagactt gcaaagaagg tatatgagat
     2881 aaatga
//
LOCUS       DAVGJJ010000123         2859 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000123 DAVGJJ010000000
VERSION     DAVGJJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.124"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..677
                     /locus_tag="VVE35_001925"
                     /pseudo
     CDS             1..677
                     /locus_tag="VVE35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            674..892
                     /locus_tag="VVE35_001926"
     CDS             674..892
                     /locus_tag="VVE35_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062052.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(949..1233)
                     /locus_tag="VVE35_001927"
     CDS             complement(949..1233)
                     /locus_tag="VVE35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU3062053.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1287..2222)
                     /locus_tag="VVE35_001928"
     CDS             complement(1287..2222)
                     /locus_tag="VVE35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU3062054.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2326..2769
                     /locus_tag="VVE35_001929"
     CDS             2326..2769
                     /locus_tag="VVE35_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU3062055.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
BASE COUNT          848 a          525 c          633 g          853 t
ORIGIN      
        1 tagtaaaacg aaacaagaac agggcaaatc gatcaggaca gtcaaatcga tttctaacaa
       61 tgttttagaa gtagaggtgt actattctag tttcaatcta ctatatttcg tctgatgggc
      121 aaatcttata aagagattat agatctttta gactgtaatc aaacaacgat ttggcgaaat
      181 gtaaaaaaat atgaggagtt cggactcgac tctctccttc aagaaacacg tggtggtcgt
      241 aaccatgcat atatgacagt tgaggaagag aaagcctttc ttgcccgtca tttgaaggct
      301 acagaggcag gagaatttgt tacaattgat gccttatttc aggcttataa aaaggagtgt
      361 ggatgaacgc ctttttagaa gagctttcac aagcttatcc ttttattcgt tatggacaat
      421 gctatatggc ataaatcaag taccttaaat attccgacta atattgattt tacctttatt
      481 cctccataca caccagagat gaacccattg aacaagtgtg gaaagagatt cgtaaacgtg
      541 gatttaagaa taaagccttt cgaactttgg aagatatcat gaatcaactt caagatgtca
      601 tacaaggact ggagaaggag gtgataaagt ccatcgttaa tcggagatgg actagaatgc
      661 tttgtgaaag cagatgagta taagtcctcg ttttgaaacc ttagagcagg ccattgcctc
      721 caaagatttg gaaaaagtcc gagaagcttt taagaaaatg aattccactt ggaccatcaa
      781 tgaaagtgtg gttcgtgaca atagtacagc tcattatgga cgtgttgaaa cagctatttc
      841 cttcctgcct agtagcatgg aaaccgaacc tacagatgag tctggaacat gaaaacagca
      901 gttgatttca agaaacaaaa aagaacagcc gaaactgttc tcttatcatt atttgagacc
      961 gtattttttg ttgaaacgat ccacgcgtcc atctgcttga gtgaactttt gacgtccagt
     1021 gtagaatggg tgtgagtctg atgaaatttc cacacggatc aatgggtaag tttcgccttc
     1081 gaactcaact gtttcgttag agcgttttgt tgaaccgcta aggaattggt aaccagtagt
     1141 tgtgtccatg aagacaactg ggcgatattc tggatggata tcttttttca ttttgcaata
     1201 tttcctttct gccatggtct ctttgcgagc catagattgt taccatacta gtctatcaga
     1261 ttctgaaaag tttggcaagt attttatcag tttttaagca agttttttaa cttttggtag
     1321 atgatttcgt tttcttctag gctataggaa ttagcaccac ttgctagagg gtggcctcca
     1381 ccatcatgct ccttggcgat ttcattgata ggatggattt tactgcgtaa gcgaactcgg
     1441 tagtggccat cagcctgttc gacaaaaatt ccccagagac tcactctgtc aatgcgtcca
     1501 ggtgcaccga caatggctgc agtttcagca tcggttacat tgtattgttt caagacttct
     1561 tgactcagga taacgcgagc agcaccattt tcatccactt ccagatggtc gtagatgtag
     1621 ccttgcagtt tagcaatttt gtagctcata gtgtccattt tgcgagtgag agccgcaaag
     1681 tcaaagttat gttctctcaa ataagcagcc aggcgaagag tccgtgcagt ggtagaaggg
     1741 tagaggaagc gacctgtatc accgacaatt cctgcaaaga gcaactcagc atcgcgatct
     1801 gccaaggcta gttgggttgt ttgggcaaat agggtaatca tctcgctagc gctacttgaa
     1861 ctagtatcga cccaagacag gtcaccgtat acatcatcat ttggatggtg gtcaatctta
     1921 atgagaaaat caccttgact atagcgctta tcatcgatac gagcagtatt agctgtatca
     1981 cagacgatga caagtgcgcc ttggtaggct ctatcttcaa caagatccat ctcagccatc
     2041 caagtaagag ttggttcatc aaaaccgacg gctttgatgg ttttttctgg gaaatgatgt
     2101 tccagcaagg ctttcaatcc cacctgactt cccaaggcat cagggtctgg tttcatatga
     2161 cgatgaatga taatggtgtc gtattcttta attttttcta aaatttgttg gcaaatctcc
     2221 ataaataacc tcaattcttt ctcatttcat tgtagcacaa gaatttttat ttttaaaatg
     2281 aaaaagatgt gatataatgg agaaagataa attgaggagg acgatatggc attagcaaaa
     2341 attgtatttg ccagtatgac cggtaatacc gaagaaattg cagatattgt agcagacaaa
     2401 ttacgtgact tgggcttgga tgttgatgtt gatgaatgta caactgttga cgcttcagac
     2461 ttcttggaag cagacatcgc tatcgttgcg acctatactt atggtgatgg tgaattgcca
     2521 gatgagatga tggacttcta cgaagaccta gcagatctca acttgaatgg caaaatctac
     2581 ggagtggtcg gctcaggaga taccttctac gatgaattct gtaaggctgt tgatgacttt
     2641 gaccgtgtct ttgtgtcaac aggagcagaa aaaggttcag agtgtgttaa agttgatctt
     2701 tctgccgagg aagaagatat tgaacgcttg gaacaattcg cagaagaatt ggctgctaaa
     2761 gtaggataag cataaaagtg cgctgatgta atcaatcagt gcatttttct tatcgagagt
     2821 tgggatttta ctagcctaat tgatggcttt ttgatacaa
//
LOCUS       DAVGJJ010000124         2852 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000124 DAVGJJ010000000
VERSION     DAVGJJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.125"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(35..2293)
                     /locus_tag="VVE35_001930"
     CDS             complement(35..2293)
                     /locus_tag="VVE35_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU3062056.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRSSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2522..2752)
                     /locus_tag="VVE35_001931"
     CDS             complement(2522..2752)
                     /locus_tag="VVE35_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU3062057.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          756 a          596 c          592 g          908 t
ORIGIN      
        1 aaaaatttca ttttctactc cttttctata ggatttattt ttctttttca gaacttttaa
       61 cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata actgctttga
      121 gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca atatagtctt
      181 gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg tcaaccaact
      241 tttccttgac cttatcagtt acatccaaac gaatgttgtt gctagagagg cgcttgttaa
      301 catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga gccttaaatt
      361 cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg agttcaccga
      421 ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct tcggtctttc
      481 ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg gtgcgtcctt
      541 gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata acatctgggt
      601 gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga actttttcag
      661 ttaattgacc agcctcatca tagccaacat aacctggagg ggcgccgacc aacttagcca
      721 cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca gcagaaccaa
      781 aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt ggcccaacga
      841 agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta cggcgaatag
      901 ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg agatcttcgg
      961 ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg atattggttt
     1021 tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc tggtctgtga
     1081 tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag gccgccttct
     1141 caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc aagcgctgat
     1201 caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca gcttcatcta
     1261 ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg gaaagagttg
     1321 cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg tatttctttt
     1381 gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact ttaacaggct
     1441 gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg agggtagtag
     1501 caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt cctgcgtcca
     1561 tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg ataaagagga
     1621 tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct tcaaattgtc
     1681 ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact tgtttacctt
     1741 ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg acaacggccg
     1801 tttttccgac accaggttca ccgataagga caggattatt cttggttcta cgattgagaa
     1861 tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg tctccacgac
     1921 gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct tggctaggtg
     1981 gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca ccgtaacctc
     2041 cacctgattg ggttggggga ataggaggag tattgctaga agatctgaaa ttgtttagat
     2101 cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc atacctttga
     2161 agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag aggtcaattt
     2221 gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta attttacagt
     2281 tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt aaaggtcaaa
     2341 aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa gtaaaaagat
     2401 tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat aaaaaaatcc
     2461 tatttgtgag acaaaaagga ttttacttag acatacaggg acaatcccag cttgttttag
     2521 attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca aacgtatacg
     2581 tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg tcgtggctgt
     2641 acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa tttccgccat
     2701 cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc attttgattc
     2761 tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac tagaaaaaac
     2821 taagatttct cgctaattct gctagtttaa ag
//
LOCUS       DAVGJJ010000125         2771 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000125 DAVGJJ010000000
VERSION     DAVGJJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.126"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(16..2607)
                     /locus_tag="VVE35_001932"
                     /pseudo
     CDS             complement(16..2607)
                     /locus_tag="VVE35_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          868 a          650 c          417 g          836 t
ORIGIN      
        1 ttttaaaaat tttctctatc gtaaacctgc cactagttca gaagctatac tagctgattc
       61 caaacgtaat atggtcatct catctacctt ccctaataat ggcattgcac cataccaaac
      121 aatagtctta ttcaccaaaa gaataggcgt tactattttt gattgagtaa aagacacttt
      181 aattttctgg ctcttcaact gatctagcca tgctttattt acaaccgtct ctggtataca
      241 tatctcaatt tgactaatcc taaattcttt tagaaaattc atcagttttg tctggtgcac
      301 ataaggtaaa ataagcatac attcctgtct ttcccctgct aagtcctctc tcgacacctt
      361 ctcatatcta ctatcaacat aaacgaattg tttctcctca ccctcaatgg cacgataatc
      421 catctttcga taagctactt gtcgtttctg aaacatcttt tctaaataag gaacatgaat
      481 atccacataa tcgaaaatac gcaccaaaga cttatccttg tagtttctat gaatccaacc
      541 tgcgtactga atcaaattat ttttccaaga aaagggtgct gccaagataa gcgtgtccat
      601 ctgaggtaag tcaaaacctt cgccaatgta ttttccagta aacaacaaaa taaacccttt
      661 atctaactgt tctaacgtct ccagtaaact cgttctcttt ctcggacttt ggtttttccg
      721 ctaataatgt aacagtcatc aacctctttc tctttcaata acttttcaaa gacatctatc
      781 tgttgaattc gattaactaa aaccaatata ttccgtcctt ctgccacttg ggctagaata
      841 tccttgagaa tcaactgatt cctcgctgag tctgtagcaa tccaatcact gagctgtata
      901 aaattacttg ctttggtctt ttcaatttct ctgaaaaaca ataggttcat gaccattctt
      961 acgctcaggc gtagccgtca aaccgtaaag atacttccct ctaaactgag caacaacttt
     1021 ttcaaacatc aagacagaga tatggtgaca ctcatccaca atcatcatct catactcatc
     1081 caaaagactt tgactatttt ccaacttaaa tagagattga atcataacaa catcaaccag
     1141 tttactcagc catttcttag tcccaccgta ctgtccaaca tagccgatta ccttttcacg
     1201 acctgatgcc gtataacgga tagcctcctc ctcttcgaaa gtcaaaaagc agtttaagcg
     1261 atctagccat tggtctaaga gttgcttatt atggactaga ataattgttt ttgttttccg
     1321 ttcagagata agagcagcac ctaaaacggt cttcccaaaa ccagtctccg catgaagtaa
     1381 accattttct ttagaagtca tatctgacag ggctaactct tgctcaaaag tgagttctcc
     1441 cttaaattcc acgctaatag acctttgtac ctttctccta tcttccacag atacctgctt
     1501 aaatttatct tgcaatggat acagcaaacc tcttggcaac cataaataat gatcggattc
     1561 tccaaataaa tacattctct caggaatttg ataggttggc tgtcgcatag cctgctttaa
     1621 ataaaattcg ggattagaaa atgaagccat atttttcaag aaaaacagtg tcttgggtgt
     1681 cacagacgat ttttcaagtt ggatcatatt ggataagacg atcttcaact ccttgtccaa
     1741 ttcttgcttg cctaactctt cttggattaa cagtgccact ttagcagttg aaaccctctg
     1801 aatttcttgt agatacctcc attggtcttc ataaggctga aactgttcat ccacaaagac
     1861 cgttcgccct tgatggtaag cttctccttg aaaaggcaag gcaatcaaat ttccaaatcc
     1921 ccccttagga aggacatcct gatttggaaa catgcgatca aaagaatcaa aggacagttg
     1981 catactttcc tgcattgcca attctaatag tttctttcca aacaagcgag cctctcgact
     2041 cggaatagct tcctcaaaga agaaccaaat atggagtccg tgacccgaac gagaaatctc
     2101 tagatgggct tccatctggc gttccctggc tattcttcga atagttaaac cagcttcttt
     2161 ccaatctcct tcatcaaaat ccagtaccaa aaaataacag ctatcatcta agtgcatagg
     2221 aaagataccg atagctgtct ccccacgaaa atgagatttc aagacggagt ccgtcaatgt
     2281 ctggaaactc cgtttttcag gtggcaactg cttccaacca taatcatagg atggaaagta
     2341 ctgaattttc ccttgatcat tgataaaact cttagcataa acatcataac ggccagcaaa
     2401 cacggaggta aacaattgta atttttcttg cgtcgtcaag tgactacttt gaagttggaa
     2461 catctcctcg aaccaagagc gttccatcac aaactgactc ttgtctttaa gacgtttaca
     2521 caagaaaaac tcactatccc catcaaaaga agaaaagaca tcaagtactt ctaccacaat
     2581 tccatccaaa gacacaaaaa cagccataaa agtcgcctcc ttgattccta taggctgatt
     2641 ataacaagaa tagctgaaat tgtacacgaa aataaaatcc taatagtact cattttgtat
     2701 gtggctaata ttccgtctcg ctccggaatg tacgaggtaa atagagttta aacgagcagt
     2761 ctataaaatt c
//
LOCUS       DAVGJJ010000126         2740 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000126 DAVGJJ010000000
VERSION     DAVGJJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.127"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            58..600
                     /locus_tag="VVE35_001933"
     CDS             58..600
                     /locus_tag="VVE35_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU3062058.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(641..714)
                     /locus_tag="VVE35_001934"
     tRNA            complement(641..714)
                     /locus_tag="VVE35_001934"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Asn,seq:gtt)
     gene            complement(720..791)
                     /locus_tag="VVE35_001935"
     tRNA            complement(720..791)
                     /locus_tag="VVE35_001935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(756..758),aa:Glu,seq:ttc)
     gene            complement(843..1595)
                     /locus_tag="VVE35_001936"
     CDS             complement(843..1595)
                     /locus_tag="VVE35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU3062059.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1592..2533)
                     /locus_tag="VVE35_001937"
     CDS             complement(1592..2533)
                     /locus_tag="VVE35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU3062060.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
BASE COUNT          936 a          508 c          401 g          895 t
ORIGIN      
        1 acaagataaa aaatctattt ttagacatat agtctaattt gtttacaagg aggaaatatg
       61 caagaaagta acaaacgctt aaaaacaaag cgaactattg aaaatgctat ggtacaatta
      121 ctgatggaac agccatttga taaaatttct actgtcaaat tagtagaaaa agccggaatt
      181 agtcgttcca gcttctatac tcactataag gataagtatg atatgattga gcactatcaa
      241 agcaagctat tccatacatt tgaatatatt tttcaaaaac atgctcatca caaaagagac
      301 gctattttag aagtttttga atatctagag tcagaaccac ttctggctgc ccttctttct
      361 gaaaatggga ctaaagaaat ccaaaatttc ttacgaaata aacttcatat catgcttagt
      421 acagatttac aaaagcgatt tatgcaactg aatctaaata ccactgaatt agaatatagt
      481 agcatctatc taactcatgc actttttggt gtctgccaaa cttggattgc acatggaaaa
      541 aaagaaagtt ctcaagaaat aacagacttc cttatgaaaa tgcttggtga tacaaattga
      601 tacaaaaaga ggaacacagc tgtgttccct tttatctata ctccgccagt aggactcgaa
      661 cctacgacat catgattaac agtcatgcgc tactaccaac tgagctatgg cggataaaat
      721 agtccgtacg ggattcgaac ccgtgttacc gccgtgaaaa ggcggtgtct taaccccttg
      781 accaacggac cttctatctg tagcagatat aaccattata tcaatttctt gctaattgtc
      841 aatcactttt gagatttttt ctctaaaata tcttttaatt ttctaatttt taatcttgaa
      901 ataggacaac gatggtcttc atagaaaaca atttctaagt tttttcgatc aatttctctg
      961 atattgccta tatttaccaa aaatgacttg tgaggagaat aaaatcgctg agtatgtttg
     1021 tccttttcct gaatatctgt catggtacca taaaactctt ttgcaaaatt cttaccaata
     1081 atgcgcaatt tatgagagac ccctgttgtt tcaatataca aaatatcatg gtaaggaatt
     1141 tttaaatcat ttcctttgta attgtagtcg aaataatcta caacatcttc attttcaagt
     1201 aacatactct tcgtgtagaa gatattttgc tcaattctct tcttaaacat ctcatcattg
     1261 atatccttat caacaaaatc tagggctgat acctggtatt tataggttag agtcgcaaac
     1321 tctgatcgac tagtgataaa gacgataata gcgtaaggat tgtaatgacg aatgagctga
     1381 gccacttcaa atcccttttt ctcaattcca tgaatatcga tatctaggaa ataaagttga
     1441 tttacttcat cattttcaat gtattcttca aattcacgga cttttcccgt tgtcttgtat
     1501 gatattggaa tattcgattc tttcgaaatt tcatccaata ttctctctag tctcacttga
     1561 tgttcaataa catcttctaa aattaaaact ttcattcaaa ttccctctta aatctaatga
     1621 tttgtctaaa tgtactgcct tccatctctg tttctaaaat aatattgttg tacttatcta
     1681 gtagttcttt cacattattt aatccgactc cgcgatttct tcccttagtg gagaatccta
     1741 aggcaaatag atctcctgaa ggagtcatcg tcattttaca tgaattctga atcacaataa
     1801 ctgtttcagt ttccatctta ataactgcta cttccatctg ctttttatag ctatcagccg
     1861 atccttcgac agcattattc aataaaacgc tcatgatacg aaccaaatcc aatagttcaa
     1921 ttggaagctt ggtaatcgta tcttttactt ccagtgtaaa ctctacacca ttatttcgag
     1981 catagacaat tgactgagca accaaacttc gtaaagctga gtcttctatg ttgttcaaat
     2041 caaagtaagt gtacttatct gaacgcaatt tatgatttgc tttgactaaa acttcattgt
     2101 aaattctgtc aatttcctgt aaattaccac tgtcaattgc catctgcatg ctgacaagca
     2161 ttccagcata atcatgtcga aaaccacgga tttcattata cagaccaaca atttcatctg
     2221 tgtaattctg taaatgtttc tgttcaaatt tcttctgctt caaagcaatc tctttctcca
     2281 tttgaacttt atgagaattc attgcaaaga aggtcaaaag gagagagata aagacaatag
     2341 atgacaaaat acttccaaaa ctattcaaat gtttaatcgt acttaccata tctgaaacga
     2401 aagatacaat atgtagcaat agtaaagcaa aaaatacttt tttcaagaaa ggataaaggt
     2461 agtcctcgtc aaaataggct agttccaaat ggaaatagta aatgattttt aatgtaacaa
     2521 aataggttaa caccgtcaca acgaaaaaga atgggaaatg atattgtaaa acaaaattat
     2581 ctcctgttat agaggagaaa gttacggaca gaaagttatg agtgctctca tataaaagag
     2641 atagtagtaa acttaggaat agtcctctat ccctctcata ctgtttcatc catcgaaaat
     2701 aggaatataa tcccaaagga aataaaaatc tttcaatccc
//
LOCUS       DAVGJJ010000127         2703 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000127 DAVGJJ010000000
VERSION     DAVGJJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.128"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..353)
                     /locus_tag="VVE35_001938"
     CDS             complement(<1..353)
                     /locus_tag="VVE35_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3062061.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEEGLQDAVKHTHGS"
     gene            complement(387..1352)
                     /locus_tag="VVE35_001939"
     CDS             complement(387..1352)
                     /locus_tag="VVE35_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU3062062.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1470..1883)
                     /locus_tag="VVE35_001940"
     CDS             complement(1470..1883)
                     /locus_tag="VVE35_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU3062063.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1880..2116)
                     /locus_tag="VVE35_001941"
     CDS             complement(1880..2116)
                     /locus_tag="VVE35_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU3062064.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(2176..>2703)
                     /locus_tag="VVE35_001942"
     CDS             complement(2176..>2703)
                     /locus_tag="VVE35_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3062065.1"
                     /translation="FGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSG
                     GIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYF
                     CQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVALVDKSQLAIQKSLE
                     ELETLKKSLMQEYFG"
BASE COUNT          806 a          539 c          380 g          978 t
ORIGIN      
        1 gaaccatgag tatgttttac agcatcttgc aaaccttctt caacaacata tttaaaatac
       61 gatttatcta tatctatctt atcaaaaacc actttaaaaa tgtgctgatt taacactgcg
      121 gatcttccac accactgaaa aacccctaat gtacccgacc acgaaataag aatatcacca
      181 gcttcaacaa tgtatttttt atcaatcgta cccgaataat aatttatccc ttttgaagtt
      241 ttagtcagat tctgtattct gataatctct ttcccttcag atgaccaatc ttgaggttta
      301 aaagcatagc cattgataaa agtagccact tgccctaatt tcactttctt catgcaacca
      361 cctcctcgga atatcgggat ttgcttttaa gagatgaatt tttggtgtga atgcttgaca
      421 ttattttgat tgaccatggc ataggccagg gttgtgtcaa tcttgctatg acctagcagt
      481 ttttggactt gttcgatagg cataccttta tcaattgcct tagtcgctaa agttcttctg
      541 aacttatgtg ggtgaacctt ttgtatgccg agtttgtctc ctaactttct taagcgaatc
      601 tctacaccag caattccaag tctctggact tttccaacta gcgttacaaa aagagcaggg
      661 tgactatctt ttctgtcgtt aagataattt cttaaatgaa ttttcgtccg agcatcaaaa
      721 tatactggtc tctccttctt tcctttacca aagacaacac actctctgtt ttcaaaatca
      781 atatctgaac gattcaactg tacaagctcc cctacacgca tacctgtcga tgctagtagg
      841 tctattattg ccaaatctct caaattttca cagttatcac gcataatttc caaatgttca
      901 tcagtataag tttccttcac attttgctca gtcttaattt tctttatccg tcgaatggga
      961 tttttaatga tatattcctc ttgctcaagc caagcaaaaa aagaagacaa tatacgccta
     1021 atattgtcta aatttgcctt actacaatcc ttttcagact ggtaatttgc taaataactc
     1081 cttaaatcat ctgttgtgag ttgtgtcaca gactctttaa tagcattaaa caagttttca
     1141 atcgtcctct ggtaataacg aattgttttt gagctacagc cctctacctg cttggcggta
     1201 ataaatagtt gcaacaggtt attattcgaa tacttctgct gcttttttcc gtctactaga
     1261 tgatgttcta aactctctct caacgctagt aattgttcat tatttaaaag aggtaacatt
     1321 tcttgcatga taattgtaac ttttgattcc ataatttctc actttcttat tcatcaataa
     1381 aaagagacaa tatcagtttc tgcattacag aattctaata ttgtctgttc atttattttc
     1441 ttgtgctcat tcaaatccag catactaggt tactttttga ccttttctct aacagaatgt
     1501 tcagaaaccc atttggagta gcttgtcatt ttttcatcag ttaaagcttc tactgcgatg
     1561 gttacacaca tttttactgc ttcttctacc gttacagaaa aacgatagcc gtttaattgt
     1621 aaaaatttga ccaaaacgaa gaaagcagtt cttttattag cattagcaaa aacatgcttc
     1681 tttatcaatt ggacaaatag tatcgttgct ttatcaaaaa ttgttggata aagagacttc
     1741 ccaaagacaa attgttctgg taagttcaca atcatattta aggcagaaga actaactgtt
     1801 tgaattttct catttggaga ataccgttga attgctaaag catttatttt ttcaatttgc
     1861 ttttctgtca gatagattgt catttttcca ccaaagcttt gaaaacatcg tcgtattcgt
     1921 caaatatttt atctaaattt aagtcaaagg catcatcaga tacataagaa acctgctgcc
     1981 ccaattcttc aggagtaaag ataatctcac cacttggtaa caatttcgct tcgtatttaa
     2041 caccacttgg aatattaaat tcacttggaa tcgtaatggt gattgaattt ccttgttttc
     2101 ttgtttttac taccatctga tttcctcctt tgaagtatta taacaaatca ttaccgtaat
     2161 tacaatggca gaatatcagc caaaatactc ctgcatcaga gatttcttca aagtttcaag
     2221 ttcttccaga gatttttgga ttgccaattg tgatttgtcg accagggcta caaagtctgc
     2281 gaactcattt tggagggcta gtggggggag ggggatggaa atatttaata aatctgattt
     2341 ggttaaacta ggtattgtta cagccttatt taacttctca aaattgtata attgacaaaa
     2401 atagaataaa tattctgagt ttattttttc taaaacaggc tctaatccaa atgctgtatc
     2461 tacattccaa aaattttctc taactaaaat aggtttattt atatttcctt tacgtccaat
     2521 aataactgaa tttttcttca ctatccaatc tttagcatat cccataattc ccccacttcc
     2581 ataaatagga aatttcccat cagcatcttc aacctgtttt tgatttttac catttcttat
     2641 tgttagaatc tcgttccatt tgctaacttt ccattctttt tcatttagaa taacatcccc
     2701 aaa
//
LOCUS       DAVGJJ010000128         2423 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000128 DAVGJJ010000000
VERSION     DAVGJJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.129"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            156..440
                     /locus_tag="VVE35_001943"
                     /pseudo
     CDS             156..440
                     /locus_tag="VVE35_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            650..1309
                     /locus_tag="VVE35_001944"
     CDS             650..1309
                     /locus_tag="VVE35_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU3062066.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVSYHEKF
                     K"
     gene            1334..2401
                     /locus_tag="VVE35_001945"
     CDS             1334..2401
                     /locus_tag="VVE35_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU3062067.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
BASE COUNT          911 a          318 c          468 g          726 t
ORIGIN      
        1 tcgcttacca acatttgctt ttatatttgc taaactttca gaaaaaaagt tggagtatat
       61 gatgctaggt gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga
      121 taacttattt gaaaaaggga aaaatgagat aaaagatcca tgtagtagat ttagcaatag
      181 gacatataaa agctttagaa aaagcatctg aaaaaacaga tgtttatatc tataacctat
      241 gctcgggaga aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga
      301 tccctattcc ttataaaata gttccaagac gttcaggaga cgttgcaact tgttatgcaa
      361 atgcagacaa agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt
      421 gtagagatac atggaattag caatcaaaaa atcccaatgg ctataattga tgatactgtc
      481 tatatgagtg ctgtattttg gaaaaattac ttaacctttt aatgaaatgc atatttaatg
      541 aaagataaat taaaaaaaat gtttgacagc gcttgacaaa ggaaacggtt tcattgtata
      601 atgtaaacac ggtgacaccg atgtcaaata taaaaaagaa ggtctttata tgaaaaactt
      661 aacaaaaatt aagttcaaag aaaatgggga atttaatcat ttccctggga atacagttgt
      721 agcaaatctt tatactaaac aagatttgat ggaagttgtt gatattattc aatcacgtta
      781 tagagaatta ccatttatcg ataagtttac tttaactcca aggaattcta ttcacatgac
      841 tgtaattgaa ttgttgtgcc atgaaaatcg tgaaacggag ttttggagca gtaatcttcc
      901 tctagataca cctttacagg aaatacatga ttactttgct aaacaacttg aaatttttcc
      961 attgttggat gaagaaattc atatgcgtgt aactgaaatg ggaaaacaaa acatactagt
     1021 tgaacctgca gatgaagctt ctgcaaagag attagaagaa attcgtactt atgtctcaga
     1081 aaaagcaggt gttcgtttcc ctaatcatga tagatatcaa ttccatattt caattgggta
     1141 tcttcggatt cctctaaccg aagaggagga agaagagttt actaaagtca gagcagaatt
     1201 aactgaaatt ttattagaga agattccaac tattactgta aaccgtattg actatactgt
     1261 atttgaagat atgagacaat ttgtttcata tcacgaaaaa tttaaataaa ataataaaaa
     1321 aggagaactt atcatgaaaa aaatgaaagt ttggtctact gtacttgcaa cgggagttgc
     1381 tcttactaca cttgctgctt gctctggagg ttcaaattct acgactgctt cttcatctga
     1441 agaaaaagct gataaaagtc aagaattagt aatctattcg aactcagtct caaatggtcg
     1501 tggtgattgg ttaactgcta aagcaaaaga agctggtttt aatataaaaa tggttgatat
     1561 tgctggcgct caattagcag accgtgttat tgctgagaag aataatgcag ttgcagatat
     1621 ggtatttgga attggtgctg ttgactcaaa taaaattaga gatcaaaaat tactagtaca
     1681 gtacaagcct aaatggttag ataaaattga tcaatcttta tcagataaag ataattatta
     1741 taatcctgtg attgttcaac cattagtttt aattggggcg cctgatgtaa aagaaatgcc
     1801 taaagattgg actgaattag gtagtaagta taaaggtaaa tattcaattt atggtcttca
     1861 aggtggtaca ggacgggcaa ttctagcaag tatcttagtt cgttaccttg atgaaaaagg
     1921 agatttaggc gtatcagaaa aaggatggga agttgcaaaa gaatactttg caaatgccta
     1981 tactcttcaa aaaggtgaaa gctcagttgt taaagcgtta gataaagaaa gcccaattca
     2041 atacggtatg atctggggtt ctggagcgtt aattggacaa aaagaacaaa atgttgaatt
     2101 caaagttatg actcctgaga ttggtgtacc atttgtaact gaacaaacta tggttttaag
     2161 cactagtaaa aaacaagcgt tagctaaaga atttattgat tggtttggtc aatcagaaat
     2221 tcaagtagaa tatagtaaaa aatttggatc tattcctgca aataaagatg ccctaaaaga
     2281 gttacctgaa gatacgaaaa aatttgttga tcaagtgaaa ccacaaaata ttgactggga
     2341 agctgttgga aagcatttgg atgaatgggt agaaaaagct gaattagaat acgtaaaata
     2401 agaaataaga gttatttttt atg
//
LOCUS       DAVGJJ010000129         2408 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000129 DAVGJJ010000000
VERSION     DAVGJJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.130"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            54..1808
                     /locus_tag="VVE35_001946"
     CDS             54..1808
                     /locus_tag="VVE35_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062068.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1774..>2408
                     /locus_tag="VVE35_001947"
     CDS             1774..>2408
                     /locus_tag="VVE35_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062069.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQ"
BASE COUNT          854 a          327 c          442 g          785 t
ORIGIN      
        1 tcaattaagc gaatttatat ctaaaaggga tattaaagaa aggagatatg cttatgaaga
       61 tttacaaaaa actatttgct tatgtccaag ataagaaata tcttggggtt ttggccataa
      121 ttttttctgc tatatctgct ctacttacag tatatggata ttatttaatc tacaaatttc
      181 tagataagtt aataattaat tcaaatttat ccggtgcaga gagcatagca ttaaaatctg
      241 ttattacact aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacatatct
      301 tgggattcag gcttgaaaca aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa
      361 gttttaggtt ctttgactta aatccatctg gtcaaataag aaagattata gatgacaatg
      421 ctgcacaaac tcatcaggtg gtagcacaca tgattcccga tagttctcag gcaataatca
      481 cacccgtact tgtacttgca cttggcttta tagtaagtat aagagttggc ataattttgc
      541 ttgctcttac tataattggt ggcttaattt taggggcaat gatgggcgag caagaattta
      601 tgaagatata ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag
      661 gaatgcaagt tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg
      721 cgataaaaga ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg
      781 ttttgtatca atggttattt tttggactga ttgcaatttt aattattcct atagtttatt
      841 ttatgactag cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt
      901 tatcaggagt tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc
      961 aaggaaatta tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat
     1021 tagtgcatgg taatatcaat aattttaaaa actataatat agaatttgag aatgttagct
     1081 ttgcttataa tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt
     1141 cctacgcact tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag
     1201 gtttttataa tgttaataaa ggaagcataa agataggcgg gatagcaata agtgaatatt
     1261 ctgacgaagc cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga
     1321 agagcattta tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga
     1381 gagccttaaa attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata
     1441 caatcatagg ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg
     1501 ctagagcaat tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg
     1561 acccagataa cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag
     1621 ttatcatgat tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg
     1681 atagtggaaa aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt
     1741 ataagagcct gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag
     1801 ttttataaaa aaagatttgc tcttacagat ggaggagcaa gaaatttaag taaagcaaca
     1861 ctggcttcat ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt
     1921 gctcaggaag ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt
     1981 ttgactttga tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca
     2041 acctatcaag aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct
     2101 ctatcttact tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt
     2161 gaaggcatag agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt
     2221 ttcccattaa tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt
     2281 attccatcta ttttaagctt tatatttata cctttatcta aaaaatatca ggttaatgga
     2341 cagaatagat attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa
     2401 atgcaaat
//
LOCUS       DAVGJJ010000130         2398 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000130 DAVGJJ010000000
VERSION     DAVGJJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.131"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..246
                     /locus_tag="VVE35_001948"
     CDS             <1..246
                     /locus_tag="VVE35_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062070.1"
                     /translation="GIIYDNEALGDLSKQYLNRNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCMPSQYDRFFYKDRPYIFSRKSPIKR"
     gene            561..1539
                     /locus_tag="VVE35_001949"
                     /pseudo
     CDS             561..1539
                     /locus_tag="VVE35_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1916..2290
                     /locus_tag="VVE35_001950"
     CDS             1916..2290
                     /locus_tag="VVE35_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU3062071.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          809 a          316 c          431 g          842 t
ORIGIN      
        1 ggaatcattt acgataatga agcattagga gatttgtcaa aacaatattt aaacagaaat
       61 atttcggaat tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcctctaaa
      121 caaatagtag ttataaatga atttatagct tgtttaaatt ccagatgcat gccatcccaa
      181 tatgatagat ttttttataa agatagacct tatatttttt ccagaaagag tcctattaag
      241 aggtgaaaat ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt
      301 atagctagat atttttgagt aactagaaat atcattaggc tacccatatg attgccagaa
      361 aagatggctg atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc
      421 tatcttgatg ggggagattg gaataatttt agtagaaaca ttaaaggatt atgggcaagt
      481 agcacaggat taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt
      541 aacaatggaa agtagactaa atgaaaaata aacgttattt ttttgacaca attttaataa
      601 ttttactgct tataagcacg atattttgtg tcagcccagt atttataaaa ttggacatct
      661 taggaacccc ctctcatgcc atcttaacct ttgtattggc gataccgtta ttctatattt
      721 tgtctcaatg cttacatact ttgctattac tagtttcctc aatcttttgt aaattaagac
      781 caatttattt ttattttata tttgtgatca ttataggagc taggaaatac tatcgaattc
      841 tctttcacca actaatggga tttagtccag gaatagcagt gttttataag gagagtcaaa
      901 caactaagaa tctgtttaaa ttttactact tcttgtactt tacaacacta atttcatact
      961 attttttctt tacttttgtg tatgacaaac cgttgctttt gcctcttatt ccattttcta
     1021 ttatcatagc attagtacaa aaactttata gaattgagaa tcaacaatta tttctgttaa
     1081 aatcaaaggt actaacaata ctggaatcta agagggattg tgagtttaat cttcaggatt
     1141 atcatgaaat ttggaaattg caatcaaaat ctgagttgcc atgcgtggct ttatcttata
     1201 tcagtttgat caagccatat ctttcagaga gcgtgcgtga acaaattgat ttattagaag
     1261 ttaagcgatt taaaaaaaat aaatcatcca atatcacttt atggtatgtt ggatgttata
     1321 aaattaaatc tatatttacg gcattataat gaaaaaaata aatatgaatc aatgttaaag
     1381 aaaattttag aggtaagacc agactttgtt ttaatcgagc agaatattga tgatagttta
     1441 aatagctcgc aaccattatc tttatcttta gccatttcag aaatccaact tttgcttgaa
     1501 gtgtatatgg ggatcaaaca tgtttcaatt aggagataaa accagaataa tatttgccat
     1561 tataggggca agttgcatat tagtcagtat gtatcttata cctctcatta acacggataa
     1621 taaattttgg gaaatacttt tatttatatt aggtgtattg ctaattggaa ttgcgttact
     1681 aagtcaaaaa aaataaattt tgaaatagta ttagtgagtt cgcaatcatt tattgctagt
     1741 ggtttagaac tgttaagttt caaacaataa ttgattttaa accttaggta acctataaaa
     1801 acaaataagg tagagaaagg atatcttatc taggtagcta agtgataaaa acacagtcgt
     1861 ctatcacttg ttaggaaact atcctaacta tgcttaataa aaaaggagaa aataaatgga
     1921 actcgtatta ccaaataatt atgttgttct tgagcaagaa gagatgatgt atcttgatgg
     1981 gggattttct attccgagat ggcctgttgc aacagccatt aatatagctt ttaatggtgt
     2041 tttaggtgga ggagcaatca gtctagttag aaattatatt cgtaattatg gtttgcggcg
     2101 agttacaagc gcaattgctg gagcagctgc aagatatgtt ggggtacgag ttgcaaatag
     2161 agtggcagga tttgcactgt ctgctattaa tggatttgca gcttggatgt caattggcaa
     2221 tgctattaca acaatctggg ccaataatga tgtaaatagg agagacccaa atttaaacgc
     2281 cttgtggtaa ctagatatgg gtattaaaat ggaaaaaatt tttgttatta ttttttttgt
     2341 atgtctattt atatcatcca taactttttt agcctatgat tttgttagcg aagaaata
//
LOCUS       DAVGJJ010000131         2335 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000131 DAVGJJ010000000
VERSION     DAVGJJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.132"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            132..944
                     /locus_tag="VVE35_001951"
     CDS             132..944
                     /locus_tag="VVE35_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU3062072.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKILQDLFL"
     gene            1283..2302
                     /gene="adhP"
                     /locus_tag="VVE35_001952"
     CDS             1283..2302
                     /gene="adhP"
                     /locus_tag="VVE35_001952"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU3062073.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINSLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT          689 a          493 c          492 g          661 t
ORIGIN      
        1 tttttcaatg tctttgtttg atttgtttaa gaaattaatc caatttcatt ttttgtttgg
       61 aatctgcttt tctgcctgct ttctgttata atagaagaca tcttatctga aaacacacta
      121 gaaaggtcca tatgacgaaa aaaattattg cagttgacct ggatggaacc ctgctcaact
      181 cagacagtca aatttctgac tttaccaaaa aaaccatcaa aaaagttgct gaaaaaggcc
      241 atcaggttat tattacgaca ggtcgccctt accgtatgtc aaaagatttt taccgtgaac
      301 tgggcttaga cactcctatg attaacttca acggatccct tactcattta ccagaccaag
      361 tttgggattt tgaaaagtgt ttgactgtag acaaaaaata tctgctagat atggttcaac
      421 gttcagagga cattcaagcc gattttatcg ctggagaata tcgtaaaaaa ttctacatta
      481 caaatcccaa tgaagaaatt gccaatccca aactatttgg tgtagaagct ttccagcctg
      541 aagatcaatt ccagcctgaa ttggtgacca aggaccctaa ctgtatcctc ttgcagacca
      601 gagccagtga caaatatgcc ttggcaaaag acatgaacgc cttctaccag catcaacttt
      661 ctatcaatac ctggggaggt ccgctcaata tccttgaatg taccccaaaa ggtgtcaaca
      721 aggcctttgc tttggactac ttgctcaaga taatgaatcg tgacaaaaaa gatttgattg
      781 cctttggaga tgaacacaat gataccgaaa tgctcgcttt tgctgggaag ggttatgcca
      841 tgaaaaatgc caatccagag ctactccctt atgcagatga gcaaatttcc cttaccaacg
      901 accaagatgg ggttgccaaa atcctacaag acttattctt ataacctata ctgatactca
      961 atgagaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca ctgctttgag
     1021 gttgtagatg aagctgacgt ggtttgaaga gattttcgaa gagtatgacc agcttgcaag
     1081 ctggtctttg tgctcttttc acagtctcat caaccagtta atcgattgtt ctactttttg
     1141 tctccaaaac cttggaaaag gctttctttt gtgatatact tcacatataa atacaagaga
     1201 gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag tcccgttgtc cctgttctcg
     1261 gtcaatatca aggaggatag ctatgaaagc tgttgttgta aatccagaaa gcactggtgt
     1321 tgctattgaa gaaaaagtac tccgtccact tgaaactggg gaagcacttg tagaagttga
     1381 atactgtggc gtttgccaca ccgacctcca cgttgctcat ggtgactttg gtcaagtccc
     1441 aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa gagattgctc cagatgtgaa
     1501 aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc tttgaaggat gtggcacttg
     1561 cgaatactgt acaactggtc gcgaaaccct ttgccgtaca gtgaaaaatg ctggctactc
     1621 agtagacggt ggtatggctg aacaatgtat cgtaactgct gactatgctg tcaaagttcc
     1681 tgacggactt gatccagccc aagcttcttc tatcacatgt gctggagtaa caacctataa
     1741 agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt gttctttacg gtgctggtgg
     1801 acttggtaac ctcgctgttc aatacgctaa aaaagtattc aatgctcatg ttatcgcagt
     1861 cgatatcaac aatgacaaac ttgcccttgc aaaagaagta ggcgctgaca ttgtgattaa
     1921 cagcctcgaa gttgaagatg tagctggact cattaaagaa aaaactgatg gaggagctca
     1981 ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac caggctgttg actccattcg
     2041 tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa atgatggaac tcagcatcgt
     2101 taaaacagtc ctcgatggaa tccaagtcat cggttctctt gtcggaactc gtaaagactt
     2161 agaagaagcc ttccaatttg gtgcagaagg tctggtagtc ccagttgttc aaaaacgtcc
     2221 agtagaagat gctgttgcca ttttcgacga aatggaaaaa ggccaaatcc aaggacgtat
     2281 ggtactcgac ttcacccact aatctaatag aaacctattt taaaagttgg aagct
//
LOCUS       DAVGJJ010000132         2191 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000132 DAVGJJ010000000
VERSION     DAVGJJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.133"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..811
                     /locus_tag="VVE35_001953"
     CDS             <1..811
                     /locus_tag="VVE35_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU3062074.1"
                     /translation="RQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTV
                     LETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGS
                     VSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGM
                     LDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYI
                     KPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDNESDENDDADY"
     gene            798..2150
                     /locus_tag="VVE35_001954"
     CDS             798..2150
                     /locus_tag="VVE35_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU3062075.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
BASE COUNT          744 a          346 c          428 g          673 t
ORIGIN      
        1 tagacaaaat ttgtcagtaa attatgatag tggtgttcca ggatggtcac tacctagcaa
       61 atatgtttac gagttgtata aagataaaaa ttataataag caagaatact acaaaagtgg
      121 tggaaaaact gtcctagaaa cattgagaaa gagatttggt ttgaaagact atgtttcccc
      181 aacagaaatt gatactgata agaaattcgt cttcatcatc gatgaaatca atcgtgggga
      241 gatttctaag atttttggcg aactcttttt ctctatcgac cctggctatc gtggtgaaaa
      301 aggaagtgtt tctacccaat atgcaaatct acacgaaact gatgaaaagt tctatatccc
      361 cgaaaatgtt tacatcatcg gaactatgaa tgatattgat cgttcagtgg atacctttga
      421 ttttgctatg cgtcgtcgtt ttcgttttgt tgaagttact gccgagggtc aagttggcat
      481 gttggataaa gagctgaata tccatgcaga agaagcaaaa attcgtctaa gaaacttgaa
      541 cgctgctatc gaaaatgttc aggaattaaa cagtcattat catattggac caagttattt
      601 tcttaagttg aaggatgtag attttgacta tgaattactc tggtccgatt atattaagcc
      661 tctcctagaa gactacttgc gaggttctta tgatgaggtt gaaactttgg aaactttgaa
      721 aaaagcattt gaactgacaa ataatgagca aaaagatcag gcagtagctg atgacaatga
      781 aagcgatgaa aacgatgatg cggattactg ataatcaaca caagattatt aaagaaaaat
      841 ttgttgaaga atatcctaaa ctaagcaatc ttcttttaga cagaaccttg gaaagtctat
      901 cccaagatga acgtattttc atttttccaa atgatttgac tcatactcca gatttggata
      961 aaaaacaaaa gattttggaa acgatcaatc aggaaatcaa gacaggaaat gtgattggtt
     1021 ttcttggata tggtcaggaa agattaacga tttcctcacg attttctgat gagagtaatg
     1081 accatttttt gcattatctc ttaaacaagg ttcttcatat caatctcact agtttagatg
     1141 ttgctttgtc tcgtgaagag aggctttatc aacttttgat gtatctcttc cccaagtatc
     1201 tacaagctgc tattcgaaaa ggtctttata aggaatatca tcgattttct cataacgaca
     1261 gtcatgttaa gggagtgatt gatgtaagaa accatctcaa gaaaaatctt cctttcacgg
     1321 gaaatattgc ctacacaacg agagagttca cctatgataa tcccctcatg cagttggtcc
     1381 gtcacactat tgaatacatt aagaatcaga aaagcattgg tcaaggggta ctagataatc
     1441 tctcaactag tcgtgaaaac gtatctgaaa tcgtgcgtgt aacgccctct tataaactag
     1501 ctgatcgtgc taagattatt cggggaaatc aatctaaacc tatacgtcat gcatactttc
     1561 acgagtacag aaacttacaa gaactttgtc tgatgatcct aaaccaagaa aagcacggtt
     1621 tagggtatca agatcaaaaa atctatggta ttctctttga tgttgcctgg ctttgggaag
     1681 agtatgttta caccttgttg ccaaaaggtt ttgtacatcc cagaaataag gataagacgg
     1741 atggaatttc agtattttct gttgggaaac gaaaagtata tccagatttt tatgacagag
     1801 aacgaaagat tgttctagat gcaaaatata aaaaactgga attgactgaa aaaggaatca
     1861 accgtgagga cttattccag ctgatttcct attcttatat tttaaaagct gagaaggctg
     1921 gacttgtttt tccgagcaag gacaaggtta ttgataatga gatgggaaac ctagcaggtt
     1981 atggagcttt gttgaagaaa tggtctatcc aaattcctca gaagtcttca tcttatagtg
     2041 cattttgtaa aatgatggaa aattcagatt atagtgcatt ttgtaaaatg atggaaaatt
     2101 cagaagaaaa ttttaaagcg attattgatg aagaagtggg gagaaaataa tttcccctct
     2161 ttcttttatt tttaacacaa gtaaaacccg a
//
LOCUS       DAVGJJ010000133         2017 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000133 DAVGJJ010000000
VERSION     DAVGJJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.134"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            19..1044
                     /locus_tag="VVE35_001955"
     CDS             19..1044
                     /locus_tag="VVE35_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU3062076.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1034..>2017
                     /locus_tag="VVE35_001956"
     CDS             1034..>2017
                     /locus_tag="VVE35_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU3062077.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTM"
BASE COUNT          702 a          288 c          361 g          666 t
ORIGIN      
        1 tatgtttaag gagtttttat gaaaaacaaa ttttttctaa tagctatttt agctatgtgt
       61 atagttttta gcgcttgttc ttctaattct gttaaaaatg aagaaaatac ttctaaagag
      121 catgcgcctg ataaaatagt tttagatcat gctttcggtc aaactatatt agataaaaaa
      181 cctgaaagag ttgcaactat tgcttgggga aatcatgatg tagcattagc tttaggaata
      241 gttcctgttg gattttcaaa agcaaattac ggtgtaagtg ctgataaagg agttttacca
      301 tggacagaag aaaaaatcaa agaactaaat ggtaaagcta acctatttga cgatttggat
      361 ggacttaact ttgaagcaat atcaaattct aaaccagatg ttatcttagc aggttattct
      421 ggtataacta aagaagatta tgacactcta tcaaaaattg ctcctgtagc agcatacaaa
      481 tctaaacctt ggcaaacttt atggagagat atgattaaaa ttgattcaaa agccttaggt
      541 atggaaaaag aaggtgatga gttaatcaaa aatactgaag ctcgtatatc caaagaatta
      601 gaaaaacatc cagaaatcaa aggaaaaatc aaaggaaaaa aagtattatt tactatgatt
      661 aatgctgcag atacatcaaa attctggatt tatactagca aagatccaag agcaaattat
      721 ttaacagatt taggtctagt tttccctgaa tcattaaaag aatttgagag tgaagatagt
      781 tttgcaaagg aaatttctgc agaagaagca aataagataa atgatgctga tgtaatcata
      841 acttatggtg atgataaaac tcttgaagct ttacaaaaag atcctctttt aggtaaaata
      901 aatgcaatta aaaatggtgc cgttgctgta attccagata atacaccgtt agcagcctca
      961 tgcactccaa caccactttc aataaactat actattgaag aatacctaaa tcttttagga
     1021 aatgcatgca aaaatgcgaa ataaaaaaca aataaaccta ggcataattt ttgtaatctg
     1081 cctaggtctt cttattacaa tatttttgtc attaaagctt ggaacaaaag aaattaatat
     1141 cagagatttt ttagcagctt ttggaatggg taatacaaat gatgatttta ttaaatcaat
     1201 tatatataaa agaataccta gaactatttt tgcaatttta gcaggttcta gtcttgccat
     1261 aagcggtgta ttgatgcaat cagttactag aaacccaata gctgatccag gtatactcgg
     1321 tataaacaca ggagcaagtc ttagtgtagt aattggtctt tcttttttag gaatttcatc
     1381 aagcataagc catataagtt ttgcaatcat tggtggctta gtaagtgcaa tttttgtata
     1441 cgcgattgct gtaagcggaa aagcaggcct tacccctata aaacttgcct tatcaggaac
     1501 ttgtgttagt atggctttaa gcagttttgt aagtttttta attttaccga ataataacgt
     1561 cttagacaaa tttagatttt ggcaaatagg tagccttgga gcagctacat tatcttctat
     1621 atctacacta ctacctttta taattttagg tcacttgata gctatattta tttcatcaga
     1681 tttaaacgct ttagctatgg gtgatgaaat ggctgttggt cttggagtta atgttaatag
     1741 gataagatca cttgcaataa ttgcaagtgt gcttttatgt tcaagtatta ctgcaattgg
     1801 tggacctatt ggcttcgtag gtcttatagt tcctcacttt tgtggcttat ttataagcaa
     1861 agatatacgc acaatgacca tttcttcatc ttttataggt gcagagctct tgcttatatg
     1921 tgatataatc ggccgtatgt taggtaaacc aggtgaaatt gaagtaggga taattactgc
     1981 aataatcggg ggtccagtac ttatttatgt aactatg
//
LOCUS       DAVGJJ010000134         2014 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000134 DAVGJJ010000000
VERSION     DAVGJJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.135"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            62..1243
                     /locus_tag="VVE35_001957"
     CDS             62..1243
                     /locus_tag="VVE35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU3062078.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1301..>2014
                     /locus_tag="VVE35_001958"
     CDS             1301..>2014
                     /locus_tag="VVE35_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU3062079.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISF"
BASE COUNT          734 a          353 c          369 g          558 t
ORIGIN      
        1 ttaagttata attaagctag tcagtttata ctttcagtaa ttcaaacata tggaggcaaa
       61 tatgaaacat ctaaaaacat tttacaaaaa atggtttcaa ttattagtcg ttatcgtcat
      121 tagctttttt agtggagcct tgggtagttt ttcaataact caactaactc aaaaaagtag
      181 tgtaaacaac tctaacaaca atagtactat tacacaaact gcctataaga acgaaaattc
      241 aacaacacag gctgttaaca aagtaaaaga tgctgttgtt tctgttatta cttattcggc
      301 aaacagacaa aatagcgtat ttggcaatga tgatactgac acagattctc agcgaatctc
      361 tagtgaagga tctggagtta tttataaaaa gaatgataaa gaagcttaca tcgtcaccaa
      421 caatcacgtt ataaatggcg ccagcaaagt agatattaga ttgtcagatg ggactaaagt
      481 acctggagaa attgtcggag ctgacacttt ctctgatatt gctgtcgtca aaatctcttc
      541 agaaaaagtg acaacagtag ctgagtttgg tgattctagt aagttaactg taggagaaac
      601 tgctattgcc atcggtagcc cgttaggttc tgaatatgca aatactgtca ctcaaggtat
      661 cgtatccagt ctcaatagaa atgtatcctt aagatcggaa gatggacaag ctatttctac
      721 aaaagccatc caaactgata ctgctattaa cccaggtaac tctggcggcc cactgatcaa
      781 tattcaaggg caggttatcg gaattacctc aagtaaaatt gctacaaatg gaggaacatc
      841 tgtagaaggt cttggtttcg caattcctgc aaatgatgct atcaatatta ttgaacagtt
      901 agaaaaaaac ggaaaagtga cgcgtccagc tttgggaatc cagatggtta atttatctaa
      961 tgtgagtaca agcgacatca gaagactcaa tattccaagt aatgttacat ctggtgtagt
     1021 tgttcgttcg gtacaaagta atatgcctgc caatggtcac cttgaaaaat acgatgtaat
     1081 tacaaaagta gatgacaaag agattgcttc atcaacagac ttacaaagtg ctctttacaa
     1141 ccattctatc ggagacacca ttaagataac ctactatcgt aacgggaaag aagaaactac
     1201 ctctatcaaa cttaacaaga gttcaggtga tttagaatct tgattgacat ctatgtaaag
     1261 aaagctttac ataagagaaa agatgtgtta gtgtagaatc atggaaaaat ttgaaatgat
     1321 ttctatcaca gatatacaaa aaaatcccta tcaaccccga aaagaatttg atagagaaaa
     1381 actagatgaa ctagcacagt ctatcaaaga aaatggggtc attcaaccga ttattgttcg
     1441 tcaatctcct gttattggtt atgaaatcct tgcaggagag agacgctatc gggcttcact
     1501 tttagctggt ctacggtcta tcccagctgt tgttaaacag atttcagacc aagagatgat
     1561 ggtccagtcc attattgaaa atttacagag agaaaattta aacccaatag aagaagcacg
     1621 cgcctatgaa tctctcgtag agaaaggatt cacccatgct gaaattgcag ataagatggg
     1681 caagtctcgt ccatatatca gcaactccat tcgtttactt tccttgccag aacagattct
     1741 ctcagaagta gaaaatggca aactatcaca agcccatgcg cgttccctag ttgggttaaa
     1801 taaggaacaa caagactatt tctttcaacg gattatagaa gaagatattt ctgtaaggaa
     1861 gttagaaact cttctgacag agaaaaaaca aaagaaacag caaaaaacta atcatttcat
     1921 acaaaatgaa gaaaaacagt taagaaaact actcggttta gatgtagaaa ttaaactatc
     1981 taaaaaagac agtggaaaaa tcattatttc tttt
//
LOCUS       DAVGJJ010000135         1861 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000135 DAVGJJ010000000
VERSION     DAVGJJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.136"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            249..1052
                     /locus_tag="VVE35_001959"
     CDS             249..1052
                     /locus_tag="VVE35_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU3062080.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHERNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVQINRPKKQLANDSGKKKCHAMKAQAIVTSQERIVSLNIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGFMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKDRSKVENILAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            1069..1647
                     /locus_tag="VVE35_001960"
                     /pseudo
     CDS             1069..1647
                     /locus_tag="VVE35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1660..1830
                     /locus_tag="VVE35_001961"
     CDS             1660..1830
                     /locus_tag="VVE35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062081.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
BASE COUNT          668 a          275 c          373 g          545 t
ORIGIN      
        1 ttgtataatg ctttttgatt ttagtctagt ataaatagcg agaaatatct atcctatctt
       61 ctagaatgtc ttccaaacga ggaaactctc gtaaacaaag aggttttaga ggcctattta
      121 ccgtggacta aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc
      181 cgtgagttct ctagtctgga gatttttcaa tagacttcct gcgaaacaaa atatggtata
      241 atagttctat gaatgatgaa gcaagtaaac aactaaccga tgcacgattt aagcgtcttg
      301 ttggtgttca acgcacgact tttgaagaga tgttagctgt attaaaaaca gcttatcaac
      361 ttaaacacgc aaaaggtgga cgaaaaccta aattaagcct agaagacttt cttatggcca
      421 ctcttcaata tgtgcgagaa taccgcactt atgaagaaat tgcggctgat tttggtatcc
      481 acgaaagaaa cttactccgt cggagccaat gggttgaagt aactcttgtt caaagtggtt
      541 ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat gcgacggaag
      601 tacaaatcaa tcgccctaaa aaacaattag cgaatgattc tggtaaaaag aaatgccacg
      661 ctatgaaggc tcaagcgatt gtcaccagcc aagaaagaat tgtttctttg aatattgcgg
      721 tgaactattg ccacgatatg aagttgttca aaatgagtcg cagaaatatc ggacaagctg
      781 gtaaaatctt ggctgacagt ggttatcaag ggttcatgaa gatatatcct caagcacaaa
      841 ctccacgtaa atccagcaaa ctcaagccgc taatagctga agataaagcc tataaccatg
      901 cactatctaa ggatagaagc aaggttgaga acatccttgc caaagtaaaa acgtttaaaa
      961 tgttttcaac aacctatcga aatcatcgta aacgctttgg attacgaatg aatttgagtg
     1021 ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctatt atcaaaaata
     1081 ccatcaggat tatataagat tgatacagga aaagttttat ttgatggtgt aaatattaat
     1141 caaatagata aaaaaatatt aagtcaaaat ttaggagtag ttccacagga ttcgttttta
     1201 ttgaacagaa gtattcttga taacataact ttaaagcacg aagttacttc acaaaagata
     1261 gaggaagttt gtaaagcagt tcaaatctat gatgaaatca tggctatgcc gatgaaattt
     1321 aatactatca tctcagagat gggttcaaat atttcaggtg gtcaaagaca acggatagca
     1381 ctggcacgtg cattaataaa taatcctagt attgtaattt tagatgaagc aactagtgct
     1441 ttagacacta ttaatgagga aagaataaca aagtatataa aaagtcaggg ctgtactcaa
     1501 ataattgtag ctcatagatt gtcaacgatt aaggatgcgg atattattgt tgtaatgaaa
     1561 ggtggtaaga ttgttgagtc aggaaatcat aagtacttaa tggatcttgg tggagagtac
     1621 tacagcttat atacaaaaag gaaatgaggt gtaaagaaaa tgaagaaaga aaatgaatat
     1681 gtaattttaa caacagcctc actaggggtg atgattggaa tagtgtttgc aattttttta
     1741 gattttccag ttgaatatgg tatttcttta ggattgttga atggaatagt attgggttcg
     1801 ctgattgttt acaaaaacaa taagaattaa gcataatttt ttgctgtaaa ctaaggagta
     1861 g
//
LOCUS       DAVGJJ010000136         1852 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000136 DAVGJJ010000000
VERSION     DAVGJJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.137"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            19..951
                     /locus_tag="VVE35_001962"
     CDS             19..951
                     /locus_tag="VVE35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3062082.1"
                     /translation="MSGAVVKNLNSDKVASILIPLPPLSEQQRIIETIESALEKVDEY
                     AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEE
                     GKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPI
                     YPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYH
                     ENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLT
                     KTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1008..1805
                     /gene="psrA"
                     /locus_tag="VVE35_001963"
     CDS             1008..1805
                     /gene="psrA"
                     /locus_tag="VVE35_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU3062083.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          665 a          271 c          327 g          589 t
ORIGIN      
        1 tctcaatttc tatctctaat tagtggagct gttgtgaaaa acttgaatag tgataaagtt
       61 gcttctattc ttatccctct ccccccacta tccgaacaac aacgcataat agaaacaatc
      121 gaatcagctt tagaaaaagt agatgaatat gctgaaagtt ataatagact agaacagcta
      181 gataaagaat ttccagataa actaaaaaaa tctattcttc aatatgctat gcaaggaaaa
      241 ttagttgaac aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa aatacgagca
      301 gaaaaacaaa aactctttga agaaggcaag attaaaaaga aagatttgga catttctatt
      361 gtttcccaag gagatgataa ctcttattat gaggaagtac cttgtgaaat accagaaagt
      421 tgggaatggg tgaggttaaa cgatataacc tcctacattc aaagagggaa gtctccaaaa
      481 tattcaaata ttcctatata ccctgtgatt gctcagaaat gtaatcaatg gtctggcttt
      541 tctattgact tagcaagatt tattgatccc gaaacagttc atagttacca gaaagaaaga
      601 ttactaagag atggtgattt aatgtggaat tcaacaggtc taggtacgtt aggacgatta
      661 gctatttacc atgaaaataa aaatccttat ggctgggctg ttgcagatag ccatgttaca
      721 gttataagag tcttatcggg agttattaat tgccatttca tctataattt tctttcctct
      781 cctattgttc agtcagttat tgaagaaaaa gcgtcgggta gtacaaaaca aaaagaatta
      841 ttaacgaaaa ctataaaaga atatttaatc cccctcccac cccttcctga acaatccaga
      901 atcgttgata aaatcgaaca atttttcgcc catattgacg cactaattta gtataatagt
      961 tttacgtgcg gtggaatttc tatcaaacat aagataggag ttttacgttg aaagattttg
     1021 aattacattt gaagaaagct ggtttagctg aaaatactgt gcgttcgtac ctttatggtg
     1081 ttcgattttt cttggagaat tacgaactca aaatggaaga tttatttgaa tacaaaggat
     1141 atttattaga caattttaaa cctaaaactg tcaatttgcg actacaagga gtaaataaat
     1201 atctggcttt tattggtcat gatgatttga aattaaaatt tgtcaaagta cagcaaaaac
     1261 cgtttttaga agatgtcatt agccatgctg attacctttt tcttaaacgt agtttgaaaa
     1321 aagatggtat tttaaaatgg cattttgtcg tttggttttt aggtgcaact ggggctcgtg
     1381 taagtgaact tattaaacta aaagtagagc atgtagaaat cggttatttt gacatttact
     1441 caaaaggtgg taaaattcgt agactgtaca ttcctaaaaa attaagaaat agttgtctta
     1501 gttggttaga atcagaaaat cgccgaagtg gttatttatt tttgaataaa tttaacgaac
     1561 caattacagc aagaggagtt gctcaacagt taaaaaatta tgctgataaa tacaaaatga
     1621 atcctaaagt aatttaccct cattctttta ggcatttatt tgctaagaat tttttagcga
     1681 agtataatga tattgccttg cttgcagatt tgatgggaca cgaaagtata gaaactactc
     1741 gaatttatct aaggaaaaca gctactgaac aacaaaatat tgtagataaa attgttaatt
     1801 ggtaaaaaat aacaggtggt caaactgact acctgctatt tttgtgatta tg
//
LOCUS       DAVGJJ010000137         1822 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000137 DAVGJJ010000000
VERSION     DAVGJJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.138"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..702
                     /locus_tag="VVE35_001964"
     CDS             <1..702
                     /locus_tag="VVE35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062084.1"
                     /translation="HQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSGKSTLLKCI
                     SSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVIENIALPLR
                     LSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSDIIFADEPT
                     GALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIFKELHRPSG
                     EELYKILEVQSTTEE"
     gene            705..>1822
                     /locus_tag="VVE35_001965"
     CDS             705..>1822
                     /locus_tag="VVE35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062085.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQMSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKVQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLT"
BASE COUNT          585 a          240 c          338 g          659 t
ORIGIN      
        1 catcaatttt tactaggaaa taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct
       61 gcttcgagag gagaatttat aagtattcta ggaattagtg gttctggaaa gtcaacttta
      121 ttaaaatgta tttctagttt gcttgaacca acaagtgggg aagtaatttt aaatggaatc
      181 aacccctata aaatcagaaa tgcaaaattg tcaagtataa gacgtaacga agtatctttt
      241 atatttcaag catacaattt aataccttcc ctgccggtaa tagaaaatat agcacttcct
      301 ttgcgattat cacaaaaaaa attaactatt aaaaatgtag aaaacttact caaaagaatg
      361 aagtttaatg ctggcttaaa cgattttgtt ggaactctgt ctggtggaga acaacagaag
      421 gttgctatag ctagagcggt tattgctgat agtgatataa tatttgctga tgagccaact
      481 ggggctttag acagcgtttc tcgtgaagta atttttgaat tattgagaga gttagtaggg
      541 gcgggtaagt gtgtaattat ggtaacgcac gatatagaat tggcctcgaa aactgatcgt
      601 gcattaatat tgaaagatgg aaaaattttc aaagaacttc atagacctag cggggaagag
      661 ttgtataaaa tcttagaggt acaatcaact acggaggaat agagatgctc agattagttt
      721 actatcaatt tttacataat aagaaacaat ggttaggcgt ttcaccagta atttttgttt
      781 cgagtttagt tatgggattg gctgttaatg gggtaattaa tgttgagaat aattcgcaag
      841 tatttgtcgg acttccagat cctaaaccaa tttttatgtt tccgatagta ttcggaggag
      901 taacattatt ctttgtgctc tctaacataa taaatatgtt ggttgaaatt tttagagatg
      961 attacgaact tctggaggtg ttgggagcaa gtcgccttca actatcattt cttgtggggg
     1021 gacagatttt tatcatttcc tcgattatta gttttattgc atatctatgc tctatatttg
     1081 ttacgtcaaa ttattattat tttttacaat actttttcgg ggaaaatata ttacctgata
     1141 tccaatttca aatgagtgct gttggatgta tcattacagt agtgttgatt tcatttttag
     1201 cctttctaag tggttgcttt tatacgttca agaagattag aaataggaag agttctaaaa
     1261 taaggcatgt tctttcaata gtaaaaagaa tactgctatt ggctggtttt agtgttatct
     1321 ggttgctttc tttgcaacaa attttccaag atagcacaat tttagcgaag gtccagataa
     1381 tattcaatat tgtaatctta gatattgtga ttatttatca attatctcct tttatacagt
     1441 catgttttat caaactactt tctataatta tatttagaaa taattttatg ttcattgttt
     1501 cgaagtggaa tttactatat cgtaagcctt atataaaatc tatcagtgct gcaattacag
     1561 gagctatatt actaatttct agttttcaaa tgatttctca gaatatacta tctcaatttc
     1621 aagatgacag tgatttagag ctaaaagtgg catttattgt ctatgtagga gcaccaattt
     1681 tgatagtact tgcaaatatt atttcaattg catttttgtc atcgcatcaa gaaaggattg
     1741 agattcagca atttgaaatt ttaggaactt ctaattacca gatggttaag attaaagttg
     1801 gtgaggctat atttttgaca tt
//
LOCUS       DAVGJJ010000138         1796 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000138 DAVGJJ010000000
VERSION     DAVGJJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.139"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            33..905
                     /locus_tag="VVE35_001966"
     CDS             33..905
                     /locus_tag="VVE35_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU3062086.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIKMNRRNI"
     gene            905..1699
                     /locus_tag="VVE35_001967"
     CDS             905..1699
                     /locus_tag="VVE35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062087.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          620 a          280 c          305 g          591 t
ORIGIN      
        1 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
       61 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
      121 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
      181 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
      241 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
      301 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
      361 taatattagg cattggtttt caagctgtaa caagagcagg cacctcattt ttattgttga
      421 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
      481 caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt
      541 tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg
      601 gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt
      661 tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct
      721 taaatattgg caagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt
      781 tgttatcaga catattctct caaaatattt taccagctag atatccagta ggtgttgtaa
      841 ctggcttgtt aggttcacca tacttaatat atttactaat aaaaatgaac aggaggaata
      901 tataatgaaa ggtctgtggt caaataatct tacctgcggt tatgatgaaa aaataatctt
      961 ggaaaatata aatataaaaa tacctgaaga aaaaatatca gttattattg ggtcaaatgg
     1021 ttgtgggaaa tcaacactca ttaaaacctt gtctcgactc ataaagccat tagagggaga
     1081 agtattgctt gataataaat caattaattc ttataaagaa aaagatttag caaaacacat
     1141 agctatatta cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag
     1201 ccgtggtcgt ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat
     1261 aataaacaga tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga
     1321 actttctggg ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag
     1381 tatcctactt ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt
     1441 agacctcttg actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga
     1501 tataaatcta acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt
     1561 tgcagaggga aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct
     1621 tgaagcaaaa attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa
     1681 gcaccatgtt agctcttaat aattgaatat tggaattcat ttaaaagtcg caaattttga
     1741 actgaccctc aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatg
//
LOCUS       DAVGJJ010000139         1712 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000139 DAVGJJ010000000
VERSION     DAVGJJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.140"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..413)
                     /locus_tag="VVE35_001968"
     CDS             complement(<1..413)
                     /locus_tag="VVE35_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062088.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDF"
     gene            complement(413..1177)
                     /locus_tag="VVE35_001969"
     CDS             complement(413..1177)
                     /locus_tag="VVE35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062089.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1273..1676)
                     /locus_tag="VVE35_001970"
                     /pseudo
     CDS             complement(1273..1676)
                     /locus_tag="VVE35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          669 a          338 c          257 g          448 t
ORIGIN      
        1 aaaaaatcaa cctcctttat ctccaaagag aagctaaaac cattactaaa tgcaattaga
       61 aaaatcaata aaaataaagt cgccgtccaa atccccgtac taagagctgc taatttgaaa
      121 ctaaaactgg taaagtgctt aattgatttc agacgaatgc gacactccaa cctattaaaa
      181 tagttattca tcaaataaaa aaagaacaat atatatgtga acggaaagca gtatactcca
      241 gtcgtcatat cttgaagtaa gactaagatc cattctaata catttggatg gattgaatat
      301 tggcgacagc gcaataaata tactgtacta gataaaacac aggatagcag taatataaaa
      361 taaaccaata ctgctaagaa atctttttta taaaattgaa caaattgttt cattatacat
      421 agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca aataaaataa
      481 aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc aaagctaatg
      541 ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca ccaaccatca
      601 tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa gcactagcta
      661 gtccaaaaat agagaacaca gcgccccaaa ctccatacca gagagtcgtc acaagactat
      721 agagcaactg attggaatca aataattctt ttaaggcacc actataatct ccaatataaa
      781 tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag gggagaaaaa
      841 agactagaaa agaagaaata attgcagtac tacccacaat agccagatac ttctttttag
      901 aaattcgaca caattgtgct gttagaaaat gactctcagc atcctctatt atctgactag
      961 aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat cctcgaatag
     1021 aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg aaagcaagga
     1081 aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat cgaggtgatt
     1141 gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct tataataaaa
     1201 atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt aaggcttact
     1261 ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa ttcttacatg
     1321 atttctaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat tgtcaccacc
     1381 tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg gggtaatatc
     1441 tgacccacta taggcgctac gaataggggt atttaacctt ttaccgccac cagtgctgtc
     1501 attattatta attccagcag aggcgttttc tttctcaagc tcactagttg tatccgaacc
     1561 ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg tagggagaaa
     1621 caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct tttcatgaca
     1681 tcgtcctcct aagaagtggt aaacaagata gt
//
LOCUS       DAVGJJ010000140         1652 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000140 DAVGJJ010000000
VERSION     DAVGJJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.141"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(92..1072)
                     /locus_tag="VVE35_001971"
     CDS             complement(92..1072)
                     /locus_tag="VVE35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU3062090.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(1085..>1652)
                     /locus_tag="VVE35_001972"
     CDS             complement(1085..>1652)
                     /locus_tag="VVE35_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU3062091.1"
                     /translation="LNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAEEI
                     CKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHVCQ
                     AFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALSQV
                     YEVITDEKLSKQNIATLENAGIMVKVVS"
BASE COUNT          548 a          327 c          179 g          598 t
ORIGIN      
        1 aaaaacaaca gataaactat ttttgtttat ataaacacaa atgtgcaatc aactaaaaag
       61 aaaaaacgat aaactgatca gtctatcatt ttcactcacc tagaatctct ctaaaagtca
      121 aatcaccatc ttcttccaaa acttttaaaa ttgtattttt atctgtaatc aaatgattta
      181 ctaaattagc acgcaagaca gaaagaatcg aagatacttt cgtatcaccg taagcaacag
      241 ctaaactttg aggaatattt tttaaatctt ccaaagagat tgctatcgtt ctttcctgta
      301 agttttcata gacttcttta ccttttgaat caaaaaatcg acaacaaatt tctcctacag
      361 ttttaacttt ggtcaattct ttaaagtcat cctctgtaag catgtctaac cattgatgtt
      421 ttcctttatt gctaaaatca ccaattccga ctacagctat atctaaatct ttccaactat
      481 ttttcaaatt ttcaaaatat cttgattgca aaataccatc tgctaacaat ttattttctt
      541 gcacaatcgt tgcattcata aatgtacact ctccatgaaa ttttctagac atttcataaa
      601 tcagtgtatt cacatggtat ttagcgtgta tgtgactagg accacctgct agaggataga
      661 agtgaacatt tcggacactt ttactgtgaa ttaaatctac taaattactt aaacttttcc
      721 cccaagaaaa gccaattttc atattatcat caattaaatt cctaaggaca cctgctgcaa
      781 cttgagaaat tctttcagat aaaattgttg gattatcatc aaattcattt ggaataattt
      841 ctaaactttc cagaccatat ttttctttta cataattttc caacttaaac atattggtat
      901 caaaattctc tatttcaatt ttaacaattc ctgcattcct tgcttctgtt aacattctac
      961 taatagaggt tctataaatt cctaattttg ctgctatttg tgactgattt aagttttcaa
     1021 tataatacag ataagcaatt ttagaaagca gtttattcct atcttgattc atacacttaa
     1081 cctcttacga aactacctta accattatcc cagcattttc taatgtagct atattttgtt
     1141 tagaaagttt ttcgtctgtt attacttcat agacttgact taaagcaaat cttcttactg
     1201 tacctctttt atcaaattta cttgagtcag ttaggacaat gactttatcc gacactgctg
     1261 aaatatattg aactacctca ctgcgcatta aatcttttcc tgtaaagccc atctctttat
     1321 cgtagccatc tgtcccaaca aaagcttgac acacatgaaa agtctgtatc atttctttta
     1381 ataaaggtcc tacagtcacc tgtgaatctt tctgaaactc accaccaaga acaataatac
     1441 gacatgaatc ataagctctc acaaaatttg ctataaaaaa tgaatttgtt acaatcgtaa
     1501 catttctttt ttgcttgcaa atttcctcag caagtaaagc acaggtcgat ccagattcta
     1561 tcattattgt ttcattatct gacaccaatt ttactgcttc ctgaacaatt tttctcttaa
     1621 tttcataatt aattgacaaa cgtacattta ag
//
LOCUS       DAVGJJ010000141         1612 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000141 DAVGJJ010000000
VERSION     DAVGJJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.142"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1129
                     /locus_tag="VVE35_001973"
                     /pseudo
     CDS             1..1129
                     /locus_tag="VVE35_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1164..1373)
                     /locus_tag="VVE35_001974"
                     /pseudo
     CDS             complement(1164..1373)
                     /locus_tag="VVE35_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1539..1612)
                     /locus_tag="VVE35_001975"
     tRNA            complement(1539..1612)
                     /locus_tag="VVE35_001975"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1576..1578),aa:Asn,seq:gtt)
BASE COUNT          516 a          329 c          275 g          492 t
ORIGIN      
        1 agtataaaaa aaattcaaac tagaattctt cttagaaaag gccgttttaa gtgctatcat
       61 tgctcaaaaa tggaggttgc tgagacttct atcgtcaaga agaatcatca aattccttgt
      121 atcatcaacc aaaagattgc tcagaagcta attgaaaaga cttctatgac cgacattgat
      181 catcagttgt ctatttcaac ttcaactgtc attcgcaagc tcaatgactt ccactttgag
      241 tgtaatttta gaaatctgcc tgagattatg tcttgggagg ttgaaacagt ccggggagtg
      301 actgtttcaa tcgggagatg gagatgagct tcattgcgca agattttgat aatcttaata
      361 ttatcactgt tcttgaaggc agaacacaag ctgtcatccg aaatcacttt cttcgctacg
      421 atagagccgt tcgttgtcaa gtgaaaatca ttacgatgga tatgtttagt ccttactatg
      481 acttggctaa acagcttcgc tttcaaattt ctaggctcag gctgaaacag tctcccagac
      541 tgtttcactc ccgaatgcta aaatcgttct tgatcgcttt cacattgtac aacatcttag
      601 ccgtgctatg agtcgtgtgc atgtccaaat catgaatcag tttcatcgaa aatcccatga
      661 atacaaggct atcaagcgct actggaaact cattcaacag gatagccgta aactgagtga
      721 taagcgattt tatcgtccta tttttcgtat gcacttaacc aataaagaga ttttaaacaa
      781 gcttttgagt tattcggaag acttgaaaca ccactatcag ctctatcaac tcttgctgtt
      841 tcactttcag aataaggaac cggagaaatt tttcggactt attgaggaca atcttaagca
      901 ggttcatcct atttttcaga ctgtctttaa aaccttcctc aaagataaag aaaagattgt
      961 caacgccctt caattacctt attccaacgc caaactggaa gccaccaata atctcatcaa
     1021 acttatcaag cacaatgcct ttggttttag gaactttgaa aacttcaaaa aagaaaggac
     1081 gaaatttgtc ctttctaaat cttcgctttc ttcaacccac tacagttgac aaagaaccga
     1141 aaaatgtctc gcctctaata tagtcaattg aaacaagaac aagacaaaag agcctcgtaa
     1201 aaggtattgc aacttggtaa tacctttttg aggtgctttt tgatatgagc ccatgttttc
     1261 ttaataggat tgtactcagg tgagtaggga ggaagaggta aaagttcatg cccaaactcc
     1321 tcgcataaaa gttctagctt acccattcta tggaatcttg cattgtccat aatatagtag
     1381 attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg attttactgt
     1441 cctgatcgat ttgtcctatt cttatttcat tttactataa cacgaaatag attattatta
     1501 cataacaaaa aagaggtcta aacctcttaa ctaaattact ccgccagtag gactcgaacc
     1561 tacgacatca tgattaacag tcatgcgcta ctaccaactg agctatggcg ga
//
LOCUS       DAVGJJ010000142         1598 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000142 DAVGJJ010000000
VERSION     DAVGJJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.143"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            100..1320
                     /locus_tag="VVE35_001976"
     CDS             100..1320
                     /locus_tag="VVE35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU3062092.1"
                     /translation="MQFIPYDDELEVINAIHKYNSNDYTVIRLTQTMLSKSIIDANGF
                     LRKMLLDNDLLDFEKLTDKVYLMANLVLEDQQHEVKISFYKANKRGDERFWISGLGEF
                     IRSSQINVNDLIYITVDNRKELTLFNVTRSIPQNNTIIQLFGQDKVEDSLNRLIPRIR
                     LIAKQGFHRNSKGVGKIAPKDAGDTLESLLGIKTNNSQEADFEGIIELKSKSSKTLDT
                     LFTLRPDFEGTPVAEFETNDRSRVSAFARLYGYDSDKHLGYKSLYITIGSEENPQNNQ
                     GFYLEVNDRVNIVELRKQENKKTILAAFWTFKALEEELHKKHPATLWVKVESKMDGEV
                     GQFKYIEAELTRSPQFMTFLSLIKSGAITYDWRGYTTPSGKYAGKNHGNAWRIKNKQR
                     SLLFGSTEIIDLNI"
     gene            1338..>1598
                     /locus_tag="VVE35_001977"
     CDS             1338..>1598
                     /locus_tag="VVE35_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HEU3062093.1"
                     /translation="MVKDNGGSIAQKGFNYQNCVISLVAIRNYNKSNFSIYVETDEDF
                     EVTYDDSYHAYIQVKGQKNMSLKKLLQETKERPSIFEKNLSSG"
BASE COUNT          580 a          211 c          318 g          489 t
ORIGIN      
        1 aaatggtaaa tattcagata tttatttttc aacaatagaa agtcttgcag atgaaatggg
       61 agtttcactg gctgatttat ttaagagagg agaaaactaa tgcaatttat tccatatgac
      121 gacgaattag aagttatcaa cgctatacat aagtataatt ctaatgatta tactgttatc
      181 aggctgacgc aaactatgtt aagtaaaagt attatcgatg caaatggttt tttaaggaaa
      241 atgttgttag ataatgattt attggatttc gaaaaattga ctgacaaagt ttatttaatg
      301 gctaatcttg tattggaaga ccagcaacat gaagtgaaga taagcttcta taaagccaat
      361 aaaagaggcg atgagcgatt ttggatatct ggattgggag agttcattag gtcatctcaa
      421 atcaatgtta acgatttaat ctatattact gtagataatc gaaaagaact tactctattt
      481 aatgtgacga gaagtattcc tcaaaacaat actatcatac agctatttgg acaagataag
      541 gtcgaagatt cattaaatag attgattcca cggatcagac tcatagcaaa acaaggtttt
      601 catagaaatt caaaaggtgt aggtaagatt gcaccgaagg atgccgggga tactctagag
      661 tcacttttgg gaattaaaac caataacagt caagaagctg attttgaagg aattattgag
      721 ctaaaatcaa aatcctccaa aacgttagat accttattta ccctacgccc agattttgaa
      781 ggaacacctg ttgcagagtt tgaaacgaat gatagaagta gagtgtcagc atttgctcgc
      841 ttatatggtt atgattcaga taagcattta ggatataaga gtttatatat tacaataggt
      901 tcagaagaaa atcctcaaaa taatcagggt ttttatctgg aagttaatga tagagttaat
      961 attgttgaat tgcgaaaaca agaaaataag aagactatct tagctgcttt ttggacattc
     1021 aaagcattag aggaagaatt acataaaaaa cacccggcta ctttgtgggt gaaagttgaa
     1081 tctaaaatgg acggtgaagt aggtcaattc aagtatattg aagcggagtt aactcgctca
     1141 ccacaattta tgacttttct ttctttgata aaatcaggcg caattacata tgactggcgt
     1201 ggctatacga ctccatcggg caagtatgct ggtaagaatc atggtaatgc ttggcggata
     1261 aaaaataagc aaagaagttt gttgtttggt agtacggaaa ttattgactt aaatatttag
     1321 taagttggag aaattttatg gtaaaagata atgggggatc aattgcgcaa aaaggcttta
     1381 attatcaaaa ttgcgttata tctttagttg cgattagaaa ctataataag tccaattttt
     1441 caatatatgt tgagacggat gaggattttg aagttactta tgatgacagc tatcatgctt
     1501 atatacaggt caaaggtcag aaaaacatga gtctcaaaaa gttattacaa gaaacaaagg
     1561 aacgaccttc tatctttgaa aagaatcttt cttcaggg
//
LOCUS       DAVGJJ010000143         1585 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000143 DAVGJJ010000000
VERSION     DAVGJJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.144"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..406)
                     /locus_tag="VVE35_001978"
     CDS             complement(<1..406)
                     /locus_tag="VVE35_001978"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU3062094.1"
                     /translation="MSADERLQKRQEELQPLMEDFFAWCRRQSVLAGSKLGRAIEYSL
                     KYKETFKTILKDGHLVLSNNLAERAIKSLVMGRKNWLFSQSFEGAKARAIIMNLLETA
                     KRHQLNSEKYLSYLLECLPNEETLVNKEVLEAY"
     gene            760..1585
                     /locus_tag="VVE35_001979"
                     /pseudo
     CDS             760..1585
                     /locus_tag="VVE35_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530077.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          485 a          346 c          274 g          480 t
ORIGIN      
        1 aataagcctc taaaacctct ttgtttacga gagtttcctc gtttggaaga cattctagaa
       61 gataggatag atatttctca ctatttagtt gatgacgttt agctgtttcc aataagttca
      121 taataatagc tcttgcttta gctccttcaa aactttgaga aaacaaccaa tttttacgtc
      181 ccataaccaa tgatttaatg gcgcgttcag ctagattatt ggaaaggacc agatgtccgt
      241 cttttaaaat agtcttaaag gtttctttat acttgaggct gtattcaatt gcccttccta
      301 gttttgaacc tgctaaaact gactgacgac ggcaccaagc aaagaagtct tccattaggg
      361 gttggagctc ttcttgacgt ttctgtagtc gttcatcagc tgacaatgtc ctcccagtct
      421 ctttccaagg caaataactg atcacaataa gctaaacctt tagctcctaa cgatgattta
      481 tccgcttgct acggttcgta gtttaccaag cttagcgtcg ccttgctcct aaaccttgga
      541 ctgctatcgc ataggcagaa ctagaggact aaagtcctaa gttactgccg caacatatca
      601 caatgcacat agccagaata atctcctagg aattctggct ctataatatt tgtagtgggt
      661 aaatccacta tagatattat ggagcctatt ttgtgtagaa aaaaagtccc atatgaccta
      721 taatgaaaag cgacaaaaca actcattaga aagattcata tgggacaatt acattttatc
      781 acaaaactac tcgatatcaa agacacaaat acccaaatta tagatgtcgt caatagggat
      841 tcacacaaag aaatcatcgc caaactggac tacgacgccc catcttgccc tgattgtgga
      901 agtctaatga agaaatatga ctttcaaaaa ccgtctaaga tcccttacct cgaaacaact
      961 ggtatgcctt ctagaattct ccttagaaaa cgccgtttca agtgctatca ctgttcaaaa
     1021 atgatggttg ctgagactcc cctggtaaag aaaaatcacc aaatccctcg tatcatcaac
     1081 caaaaaattg ctcaaaagtt aattgaaaaa atttctatga ctgatattgc ccatcagctg
     1141 gccatttcaa cttcaactgt cattcgcaag ctcaatgact ttcactttga gcatgatttt
     1201 tctcggcttc caaagattat gtcttgggat gagtatgcct tcactaaggg aaagatgagt
     1261 ttcatggcgc aagattttga taatcttaat attatcactg ttcttgaagg cagaacacaa
     1321 gctgtcatcc gaaatcactt tcttcgctac gatagagccg ttcgttgtca agtgaaaatc
     1381 attacgatgg atatgtttag cccttactat gacttggcta aacagcttcg ctttcaaatt
     1441 tctaggctca ggctgaaaca gtctcccaga ctgtttcact cccgaatgct aaaatcgttc
     1501 ttgatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg cgtgttcaaa
     1561 tcatgaatca gtttcatcga aaatc
//
LOCUS       DAVGJJ010000144         1581 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000144 DAVGJJ010000000
VERSION     DAVGJJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.145"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            245..>1581
                     /locus_tag="VVE35_001980"
     CDS             245..>1581
                     /locus_tag="VVE35_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU3062095.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQKLESALKQYNGP
                     YILSDKRWDEVPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYLYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAASGFRSWRETFPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHE"
BASE COUNT          563 a          199 c          320 g          499 t
ORIGIN      
        1 tcgttccaat atgtgcacgt tggaatgtta gtgcttatat ggggggaatc caaaggattc
       61 tcccttttta ttttaataag tctgtctagc tatagatata ttaagtttga tagaatttct
      121 tggagatttt gttatggatt ataatttcaa tttggagcat cctttctttt tcactaataa
      181 tgattatagt acagatacta gcataaagta ctaagtaagt ctgcctttta attggcacga
      241 agtaatgaat aatgatgagt gggtttatca ataccctatt ggaaaatttg ttgaaagaca
      301 agggtggaaa atacacatta gttcagagta taatagttca cacgaattgt tacaagatgt
      361 agcaaaaatt tgccatgaaa tgcgaattcc atttaaacat cttagtacag aagacaaatt
      421 tatcatgcga aacgggaaat tggttagtcg aggattttct ggtaaattta ttacatgtta
      481 tccaaatcaa aatgaattag agtcggtttt gcaaaagtta gaaagtgcct taaagcaata
      541 taatgggcca tatattttaa gcgataaacg ttgggatgaa gtaccaatat atctgaggta
      601 tggtgtgttt aggccgtcaa gggatgatga aaagaaggtt gctattgatg aattaattgt
      661 tggcgatgaa gttgttaaag atgaacgttt accagtattt aaaattccaa aaggtatagt
      721 gccccctgac tttttgaata aatggcttga caagaaagat aaaaaacagg gagactttcc
      781 ctttattata gataatgcaa ttagattttc aaacagtggt gggatatata atgctagact
      841 aaaagaagat gggaaaaaaa taattttaaa agaggctaga ccgtatacag gtctaggatt
      901 tgatggtact tattcttcag agagattagc ttcggagtgc aaagcactaa aaattctaaa
      961 tgaatggagc gagatgccta aaatatactt gtacggtaag atctgggagc acaccttctt
     1021 aggaattgaa catatgaagg gcgttccact aaatagatgg gttaccaata attttccttt
     1081 atatgaagtt gttgataaga ccaaagatta tctattgaga gtttcaaaga tagtagaaaa
     1141 actcatcgat ttaacgaata agtttcattc tgaaaatgta tatcatcaag atttacattt
     1201 aggaaatatc ttggttaagg atgaagatga gattagtata atagactggg aacaggctgt
     1261 cttttcgaat gatgaaaaag ttgtacataa agttgcagcc tctggtttta gatcatggcg
     1321 agaaacattt cccagtgaga tagattggta tggtattaga caaatagctc attatttata
     1381 catgccacta gtaactacat cagatttgac atacaactat gtaagtcaaa ccaggataga
     1441 aggaaaaaaa ctatttgagt ctttgggtta tacaagagaa catatagatt atgtagaaag
     1501 tttattgtca tatctagatt ctaaatgtcc acagattgag aatatttcca ggaaaaaagt
     1561 tttaaaaccc atgcatgaga t
//
LOCUS       DAVGJJ010000145         1580 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000145 DAVGJJ010000000
VERSION     DAVGJJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.146"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            125..1471
                     /gene="gdhA"
                     /locus_tag="VVE35_001981"
     CDS             125..1471
                     /gene="gdhA"
                     /locus_tag="VVE35_001981"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU3062096.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKKKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          489 a          299 c          334 g          458 t
ORIGIN      
        1 ttcaaaaaaa accaaaaact gaattgaaag aatttttaga aaatttctgt tttttcttta
       61 cagtgaaaaa aaattctgct ataatgtctt atgtaataaa atatgacaat aaaaggagaa
      121 cgatatgaca tctgctaaag aatatatcca aagcgtgttt gaaactgtga aagctcgtaa
      181 cgggcacgag gctgaattcc tccaagctgt tgaagaattt ttcaacactt tagaacctgt
      241 atttgaaaaa caccctgagt atatcgaaga aaatatcttg gcacgtatta ctgagcctga
      301 gcgcgtgatt tctttccgtg ttccttgggt tgaccgtgat ggaaaagttc aagtaaaccg
      361 tggttaccgt gttcaattca actcagctgt tggaccatac aaaggtggac ttcgtttcca
      421 cccaactgta aaccaaggga tcttgaaatt cctcggattt gaacaaatct ttaaaaatgt
      481 cttgactgga cttcctatcg gtggaggtaa aggtggatca gacttcgatc ctaaaggtaa
      541 aacagatgct gaagtgatgc gcttctgcca aagcttcatg actgaattgc aaaaacacat
      601 cggaccatca cttgacgtac ctgctggtga tatcggtgtt ggtggacgtg agattggtta
      661 cctttacggt caatacaaac gtcttaacca atttgatgct ggtgtcttga ctggtaaacc
      721 tcttggattt ggtggtagct tgattcgtcc agaagcaact ggttacggtt tggtttacta
      781 tactgaagaa atgctcaaag ctaacggtaa cagctttgct ggtaagaaag tcgttatttc
      841 aggttctggt aacgttgctc aatatgctct tcaaaaagca actgaactcg gtgcaactgt
      901 tatttctgtt tctgactcaa atggttatgt catcgatgaa aatggtatcg acttcgatct
      961 tttggttgat gttaaaaaaa aacgtcgtgc tcgtttgact gagtatgcag ctgaaaaagc
     1021 aactgcaacc taccacgaag gtactgtatg gacttacgct ggaaactatg acattgctct
     1081 tccatgtgcc actcaaaacg aaatcaacgg tgaagcagct aaacgtttgg ttgctcaagg
     1141 cgttatctgt gtatctgaag gtgccaacat gccgagcgac cttgatgcca tcaaagttta
     1201 caaagaaaat ggtatcttat acggacctgc aaaagctgcc aacgctggtg gtgtagccgt
     1261 ttcagctctt gaaatgagcc aaaacagtct tcgcctctca tggactcgtg aagaagttga
     1321 tgaccgtctc aaagacatca tgacaaacat cttcaacaca gctaaaacga cttcagaaac
     1381 atacggtctt gataaagact accttgcagg agctaacatt gctgcctttg aaaatgtagc
     1441 aaacgctatg attgcccaag gtattgttta agatttattt gcttaatcct caatcactac
     1501 gattggggat ttttagttga gttagacagt tgggaaattc taattaaatg aaataaaaac
     1561 tgaacaaatt gattgcggaa
//
LOCUS       DAVGJJ010000146         1576 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000146 DAVGJJ010000000
VERSION     DAVGJJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.147"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..396)
                     /locus_tag="VVE35_001982"
     CDS             complement(<1..396)
                     /locus_tag="VVE35_001982"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3062097.1"
                     /translation="MGKEIKAVYAILNYNTWEDTARLAQKVATFQHIQSVIIVDNLST
                     DDSYHYLKRLEGEKISVYQTQRNGGYSVGNNFAARKAYNMGVDILFISNPDVDIDEKD
                     SLMIAQNLYKNNSYALLSGIEYNAMKEIDL"
     gene            complement(408..1094)
                     /locus_tag="VVE35_001983"
     CDS             complement(408..1094)
                     /locus_tag="VVE35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU3062098.1"
                     /translation="MIVKSFIKKITGKTVDIHPDVPLSYILQRGINYSFGLFRGVVRG
                     IGFGQNDKRLFIGQGVSILAKRKLFVGKNVRIGKKVSIDALSKEGIHFADNVKIGDYS
                     QIIGTGSIKNMGIGLKIGKNSSFSEYSLFGSAGGITIGDNVIAGQNVRFHAENHNYSD
                     LDKLIVEQGVSRKGISVGDNCWIGAGSVFLDGSSIGSGCVVAANSVITKNFPDNVIVA
                     GVPAKIIKMK"
     gene            complement(1084..>1576)
                     /locus_tag="VVE35_001984"
     CDS             complement(1084..>1576)
                     /locus_tag="VVE35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU3062099.1"
                     /translation="NHHQRLTTTNNKIRVAYIGPDEEYKGYFDFVDFVETLDRESYEV
                     ATYGHLPNEECPSFIEQKGYFTKEMIDSVYENIDILIVPSKWKETFGLITVEALSYGV
                     NVFVSENVGSKDLLPESHVFKNQNDLIVKFLKNDIENTKLKTLDEHSIEVIQYYERVI
                     NDS"
BASE COUNT          515 a          321 c          181 g          559 t
ORIGIN      
        1 caaatctatc tccttcatag cattatattc tatacctgat agcagtgcat atgaattatt
       61 tttatacaaa ttttgtgcaa tcatcaagga atctttttca tcaatatcaa cgtctggatt
      121 tgaaatgaaa agaatatcaa ctcccatatt ataagctttt cgtgctgcaa aattatttcc
      181 aacagaatat ccaccatttc tttgtgtttg ataaactgaa attttctctc cctcaagtct
      241 ttttaaataa tggtatgaat catctgttga tagattatcc actattatca cagattgaat
      301 atgctgaaat gttgcaactt tctgtgctaa acgcgcagta tcttcccaag tattgtaatt
      361 taatatcgca taaacagctt taatttcttt ccccatttaa catcctatca tttcattttt
      421 ataattttag ctggtactcc agcaacaata acgttatctg gaaagttttt cgtaattaca
      481 gaatttgcag ctacaacaca acctgaacca atgctggacc catccaaaaa aacagatcca
      541 gcaccaatcc aacaattatc acctactgaa attccctttc gagatacccc ttgttcaaca
      601 atgagtttat ctaaatcact ataattatga ttttcagcat gaaaacgaac attctgacct
      661 gcgataacat tatcaccaat tgtgatacct ccagcagaac caaataaact atattctgaa
      721 aaagatgagt tcttaccgat tttcaagcca atccccatat tttttataga acctgtgcca
      781 ataatttgag aataatctcc aatctttaca ttatctgcaa aatggattcc ttcttttgac
      841 agtgcatcta tagacacctt tttaccaatt cgaacatttt ttccaacaaa taatttacgc
      901 tttgctaaga tagaaactcc ttgtccaatg aataaacgtt tgtcgttttg accaaatccg
      961 attccacgta caaccccacg aaagagtccg aaactatagt ttatacctct ttgtagtata
     1021 tacgacaaag gtacatctgg atgaatgtct actgtttttc cagtaatctt ttttatgaaa
     1081 ctcttaacta tcatttatca ctctttcata atactgaatc acttctatcg agtgttcatc
     1141 taaagttttc aattttgtat tttctatatc attttttaaa aatttaacta ttaaatcatt
     1201 ctgattttta aaaacatgcg attctggaag taagtctttt gacccaacat tttcactcac
     1261 gaaaacattt accccatagg ataatgcttc tactgttatc aatccaaatg tttccttcca
     1321 cttacttgga acaataagaa tatcaatatt ttcatagaca ctgtcaatca tttccttagt
     1381 aaaatatcct ttttgttcaa tgaatgaagg acactcttca tttggtaaat ggccataagt
     1441 tgccacttca taggactctc gatctaaagt ttcaacaaag tcaacaaaat caaaatatcc
     1501 tttatattct tcatctggac caatataagc aactctaatt ttattatttg tagttgtcaa
     1561 tctctgatga tgatta
//
LOCUS       DAVGJJ010000147         1528 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000147 DAVGJJ010000000
VERSION     DAVGJJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.148"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..231)
                     /locus_tag="VVE35_001985"
     CDS             complement(<1..231)
                     /locus_tag="VVE35_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062100.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINK"
     gene            complement(228..581)
                     /locus_tag="VVE35_001986"
     CDS             complement(228..581)
                     /locus_tag="VVE35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU3062101.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAEKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(637..1509)
                     /locus_tag="VVE35_001987"
     CDS             complement(637..1509)
                     /locus_tag="VVE35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062102.1"
                     /translation="MLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSLTFFVLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWQKEDSFES
                     DLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCT
                     GLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNFDFCMNREYHK
                     MKSRKLLLSNKKIV"
BASE COUNT          557 a          273 c          204 g          494 t
ORIGIN      
        1 tttattgatt aaaatgggga aactgattaa cagaatcgaa tttatggcta gacagataga
       61 tactatttta tttttcacag aatatagagg tagtgatact attgagtaga taaaagctaa
      121 aaaaataagc aaaaaatact ccgtatcatt tataatcgcc ctgaatgata aaaatatata
      181 cacccaacac acgatgatgt gtatttttcg aatcttatct acattactca tcttactgtc
      241 gcagatatga atccatccca aggataaggg ttcccgttaa tatcacaacc tctattaaga
      301 acaccatatc caatacagta agctattttt tctgctgctg tagccaatac acctgctgct
      361 gcaccaataa gccagcctcc aagacctcca aacttaatat agtttattag ttttttggtt
      421 aaaactgcag ctccagctaa tgctataaaa gttcctgaac tcatagctaa agctgcgcaa
      481 attccttgac aagcacgctt gcttaaataa gctcccccat caagatacat cattacttct
      541 tcatcaatca caacataatt atttggtaat acaagttcca tttgttttct cctccttagt
      601 tgaaattgtt tcatttgttt tacaaatagg acgaacttaa actatctttt tattactcag
      661 taataatttc cgagatttca ttttatgata ctctctattc atacagaaat caaaattttg
      721 ccttaaccaa cttgtataat agaaaaaatc taattgatta atcttgataa gctctatcct
      781 aattttatca atcttttctg accaattatg attcttacta atagaaattt catacagcag
      841 taactcgaca agacctgtac acagaagggt gttatgagaa tcttcgattt ttttcaaaat
      901 atcctcaaaa agttcactat ccgttaaaaa ggtggattgg ttcatcgcat catagagaag
      961 aagggacatt aagtcagaat agcttaattc tgatttttgt atcttatcct ttaacgtttt
     1021 cgtcaaatca ctttcaaacg aatcttcttt ttgccatttg ttttgattaa aaagaaattc
     1081 cgtcttatta aaaacgagat gattgatatc cattatagtc cagaaaaata ttgtagtaag
     1141 catgatgatt gcaaaaggaa tttttaaaat aaatactagg ataatagcaa tttcaagaat
     1201 aaaactccat ataatcctag atcttaaatc atcaggcgac aaacttttac gatggaatga
     1261 aaaatagggc gagaacctca catttacttt cttcctgcct ataaaaataa aaggaaaaat
     1321 cgtaataaga gcttctttct tgtacaaatc tttcaatgcg tatagtatgt ctaaaactat
     1381 ttttgataat atcaaaacaa aaaaagtaag actcgtacta accacaaaat caaagggaat
     1441 ttctagattc aattgcaagt tactcactaa caaatgcaac tctagccaca agacaaaaag
     1501 agggagcatt aataaaaaaa tgattagt
//
LOCUS       DAVGJJ010000148         1518 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000148 DAVGJJ010000000
VERSION     DAVGJJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.149"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(233..790)
                     /gene="sdbB"
                     /locus_tag="VVE35_001988"
     CDS             complement(233..790)
                     /gene="sdbB"
                     /locus_tag="VVE35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU3062103.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(801..1508)
                     /gene="ccdA2"
                     /locus_tag="VVE35_001989"
     CDS             complement(801..1508)
                     /gene="ccdA2"
                     /locus_tag="VVE35_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU3062104.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
BASE COUNT          506 a          309 c          256 g          447 t
ORIGIN      
        1 tactctaaga cgactctgtt ttagggctat ttgtcgtgca ctttttctga gtcttttcgc
       61 ttttgaaccc ttatatatca agaaaaagaa aaccagtgga agttagtcta agacggattt
      121 ccactgatgt cacgcgtttt tctcatacta aaagctaggt tgtgggaaac tggaagttag
      181 ttttagaagt taccaaaatc tgactacatc ttgtaatcag attttatcga ttctagttca
      241 tttctttaaa tgctgcttct gcatccgcat tactgatagc accaaattga atctttccaa
      301 tctttccttg gctatcaatt aaatattctg taggaatgct tcgaatttga taagcttgga
      361 aggtggttgc tttggtatca taaagaactg ggatatcctt atatccttgt tcctggaacc
      421 attgtgggaa ttgctcaaca gttttttcac cttgaattcc tggtgcaatg acagtaagaa
      481 tttcgaaatc acgatctggt ttcgccgcta gttccatcaa ctctggcata cttttcttgc
      541 atggaccaca ccatgaagcc caaaacttca agtaaacctt tttaccctta aaatcagata
      601 acttaacttc tttgccatcc atggattgca atgtgaagtc tggagcatct tttccaacag
      661 caatttgttg tacagtcgtt tgttgttttg gctgttgtgc tgcttgagtc tttttagttt
      721 cttcctcacc acaggccatc aatacaacta atgacaagag acttaagcca gcaaacatta
      781 cttttttcat ttgtcctcct ttattcaaaa attccagcta gaacatttac ttgtcctaat
      841 agtaacaaaa ttcccattaa aacaatgagg aaaccaccaa ttttctttag tagcatcata
      901 tgacgcttga ttttactaaa atatggcatg actagacctg aagctagtgc caataccaag
      961 aaaggaaggg ccatgcccag agtgtaaatg agagtataaa tcgctccttg ccaagcgcca
     1021 ttgcctccag aagccgcaag tgctaaaaca gaacttaaaa ctggaccaat acaaggtgtc
     1081 caaccaaagc taaaggtaat accaagtaaa aaagctgacc aataacgatt agaatctgat
     1141 tttttaaagg taaaactttt ttgaacttct aatttcttca aatgaaaaat ttccatctgg
     1201 tgaagaccca aaatgataat aatagctccc atgccatatc gaaaccaatt tgcatagaga
     1261 atatgaccaa agtaaccagc accaaagcct agaataaaga aaatgagaga gataccagcg
     1321 ataaagcaaa gtgttcgaat caagcctgac cagagaacct ttctcccaaa caaagaaaag
     1381 ctttttgcac tttcttgatc atccaataaa atcccagcat agactggcag aagaggaaaa
     1441 atacaaggag aaaaaaagga taaaacacct gctagaaaaa cagagattaa aaatactatc
     1501 gtttccaata aagaacca
//
LOCUS       DAVGJJ010000149         1438 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000149 DAVGJJ010000000
VERSION     DAVGJJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.150"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1211)
                     /locus_tag="VVE35_001990"
     CDS             complement(<1..1211)
                     /locus_tag="VVE35_001990"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU3062105.1"
                     /translation="MKKILNKYYSLSNPVKASIWFTISNVLQKGISMITVPVFTRVLT
                     TEQYGVYSVYQSWYSIIGVLATLNLYYGVFNNGMIKYEKDKNVFTSSMQGLTTTVTAI
                     FLLIYLIGIDFWNSLLGLPTLLILVMFFDLFFTPAYSFWVARQRFEYKYRNLVFITFI
                     IAIGSPIIGISAVVLSTYKAEARVISFVLVQSCIGLYFYILNLYRGKHFFCKKYWLYA
                     LNFNLPLIPHYLSQSVLNQSDRIMINSMVGMGEAAIYSVAYSISILMVLVTSAINSSF
                     IPYTYKCIRDKKYTELGKSANLLITLVGIGSILTISLGPEIIQLFAPKQYYEAIWIIP
                     PVALSVYFMFLYPIFGNIEFYFEANHFVMWASIGGAITNIFLNFIFLKHFGYIAAGYT
                     TLFCYILFALG"
     gene            complement(1229..>1438)
                     /locus_tag="VVE35_001991"
     CDS             complement(1229..>1438)
                     /locus_tag="VVE35_001991"
                     /inference="COORDINATES: protein motif:HMM:NF016357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU3062106.1"
                     /translation="VDRSLYFPYENVTPGEKCQNFDELLVTLQNLYVGKDDYREEREN
                     IKTFFYSSETQKSVSEKIINHVLNL"
BASE COUNT          555 a          235 c          196 g          452 t
ORIGIN      
        1 tgtcccaacg caaataaaat ataacagaat aaagttgtat agcctgccgc aatatatcca
       61 aagtgtttta aaaaaataaa attcaaaaaa atattcgtaa ttgctccacc gattgaagcc
      121 cacattacaa aatgatttgc ttcaaagtaa aactcaatgt taccaaaaat gggatataag
      181 aacatgaaat atactgacag agcaacagga ggaataatcc atattgcttc gtaatattgt
      241 tttggtgcaa ataattgaat gatttctggt ccaagagaaa tagttaaaat cgaaccaatg
      301 cctaccagag taattaaaag atttgcactt ttccctagtt cagtatattt cttatctcga
      361 atacacttat atgtgtaagg aataaaagag ctgtttatag cacttgtcac taaaaccatc
      421 aaaattgata tgctgtaagc aacactatat attgctgctt ctcccattcc taccattgaa
      481 ttaatcatta ttctatccga ttggtttaaa actgattgcg ataaataatg aggaataaga
      541 ggtaaattaa aattcaaagc atagagccaa tattttttac aaaaaaaatg tttacctcgg
      601 taaaggttca aaatatagaa atataaccct atacaagatt gaactaacac aaatgagata
      661 actctagcct ccgctttata agtcgataaa acaactgcag aaataccaat aataggactt
      721 ccaatcgcta taataaatgt gataaatact aagtttctat atttatattc aaatctctga
      781 cgagcaaccc aaaaagaata agcaggggta aaaaacaaat caaagaacat cactaagatt
      841 agtagtgtag gtaagcctaa caacgagttc caaaaatcaa tccctattaa atatatcagt
      901 aaaaatatag ctgtaactgt tgttgttagt ccctgcatag aggaagtaaa gacattttta
      961 tctttctcgt atttaatcat tccattatta aagactccat aatatagatt tagtgttgcc
     1021 aaaactccaa ttattgaata ccatgattga tatacagagt atacaccata ttgttccgtt
     1081 gtcaatactc tagtaaaaac tggaacagta atcattgaaa ttcctttttg aagaacattg
     1141 ctaatagtaa accaaattga agcttttaca ggattagata aagaataata cttatttaat
     1201 atttttttca tgacagattc caataaagtt atagatttag tacgtgatta attatttttt
     1261 cagaaactga cttctgagtt tcactagaat aaaagaaagt tttgatattt tcacgttctt
     1321 ctctataatc atcttttcct acatatagat tttgcaaagt cactaataat tcatcaaagt
     1381 tttgacattt ctcccctgga gtaacattct catatggaaa atatagcgaa cgatccac
//
LOCUS       DAVGJJ010000150         1429 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000150 DAVGJJ010000000
VERSION     DAVGJJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.151"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(409..1302)
                     /locus_tag="VVE35_001992"
     CDS             complement(409..1302)
                     /locus_tag="VVE35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3062107.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNVAEAESFYEKAISLAEL
                     LDDQVLVQKILAEKQIDFPTT"
BASE COUNT          479 a          293 c          156 g          501 t
ORIGIN      
        1 tactacattt tcctagatta tcaaattact tttatatcac taagactcaa taaaagataa
       61 tgtctattta acttttatac gattactacc tcctttttag tttacacttc ctacatcata
      121 aataaacata ctcaaccctc acaatctcca aataactctt ctatatcatt gaaaattgag
      181 tttcatcctc tatactaatg taggaagcct attaaaacat tcctttaatc caatatagga
      241 ataccttatg ccattaatct ctacctgata ttatagatag aaccagactc ctatacttaa
      301 aaggacaaac aacgttccaa ccataataaa cttcctttct ttttggaatc gttttcttta
      361 tgagtttagt ataacaaaaa catgaaaaaa aacaacatga cttagaagtc aggttgttgg
      421 gaaatctatt tgtttttctg ctaaaatttt ctgaactaat acctgatcat ctagcaattc
      481 tgctagagaa atcgcctttt catagaaact ttctgcctct gccacattct tatctacaaa
      541 taaagcatat ttcgccttta acaaaaatag atgaggtcgg taaaaaacat gtcttgtttt
      601 agcaataatt ttctcaaatg tttgaatata tatcagacta tcttctgtct gaatttggat
      661 aaataggctc agaagaactt tttccatctg aactaggcta tccttatctt catattctga
      721 acatagcaat actttttgag ctaactcctc aaaatgttct ttatcttcta atcccatagc
      781 acaacaaaag aaatacaatt caataatcaa caaatcattt acagaaaaat atttcttttt
      841 taataactgc tggaaatatt cttccactaa ccctaaaccg taggtcacat ctgaactacc
      901 ataaatatca aaacgagctt gtgtcacatc cacaattaac tgctcttcct caggcaaatt
      961 ctcataatag ttttcatata tttcctcaag tatagcttct tttcgttcga ttcttccctt
     1021 atcagcgtag gtttgagaat gaatcagctc atctttgagt tttaggaatc cttttggaat
     1081 ctctataacc ttaacatcca caatattctg taatgatact ccaagtttat ccgcaatcaa
     1141 taataattta ggaagtgttg ctaaagcttg cccattttca atacgtgcta gctgacgaat
     1201 cgtcaactct gattcatcac cacaaatatc tatctgagtt tttccacaag atcgcctaat
     1261 agttttgatt ttataaccaa tttcacgttt caactcatcc atagtaatac tccttaagtt
     1321 gattacaaag atttcttttt cttcctaatt cttttgtcaa tcatatataa aacgataagc
     1381 gaaaacagcg gtagaacaat ataactcttt tcactcatta tcagctgaa
//
LOCUS       DAVGJJ010000151         1423 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000151 DAVGJJ010000000
VERSION     DAVGJJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.152"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..686)
                     /locus_tag="VVE35_001993"
     CDS             complement(<1..686)
                     /locus_tag="VVE35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062108.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLL"
BASE COUNT          542 a          253 c          183 g          445 t
ORIGIN      
        1 tgaagcaagt aggagtcaag taaattttca aaaatagcta acaatatgat tggtgtaagg
       61 tttttgtatt cacatgtcaa gtattccgag tatcgtgcag atgatatttc ttttatataa
      121 ctttttgaaa gcaagatttt ttcaaattca tcatctatta ttaatcttct atttccaatc
      181 caagcagtat taccacccaa ataagattgt gcaaatagca atatgtaaga aaacatgaac
      241 gtaaatactg aaagaggtag aattctatgc gtaaaaaaat agacgataat ggtagccagg
      301 agtaaagcta tctctcgtga caatcgaatt cttttcagca tcttttttaa cagtccgtaa
      361 ggttgtcctt ggcaatattc ttgaactaaa ttaatcggaa tgacatctct caccatttct
      421 ggaaagtaga gtaagtttat aggttgaaat ctccagctcc catcataagt aaatggatat
      481 agaaccaaat acttaaaaga tttttttctt ataaacttaa ttatcacaat atgaatgact
      541 gttgcaatga agtatattaa atataaaagt aaaaccgcca tcaatatact tacaaaaatc
      601 cccttggact caatcaatgg aaaataaaat attgcaacta gaagcgaata aagtcccgct
      661 aataaatatt tctgatcatc tttcattcaa aacaccatat tatttactaa gaatacatca
      721 atacaataga gcaactctgt aagtcaatat ttgataaagc aaagctttaa ccccttcccc
      781 agacgttagc ttttcttcaa cccactacag ttgacaagga gcccaaatta ttgataaaaa
      841 ctttagtcat catcgtctct ataaaaatat tttctataca ctcttgataa taccaaaatt
      901 aatgccaata tcataccaag agctataata atagaattta ttagatttat tttagtatat
      961 ccaatcacta gtccaataat tggactggta gacatgctta gatataataa aaataacaaa
     1021 aaatgaattt caccaattcc tgcaaaaaaa tgtttcatag ctatagtttt acgcccataa
     1081 atacccatca aatttaccgt ctacgatatc taaaccatat gcaatggctc ctcctaatga
     1141 gaagacttca gcaacagtag ccgcaatatt aagagcatta gtaatagttg ccatactaac
     1201 tccaccaata tatcttgtta tagcacttag ttacctagat aagatatcct ttagcttgct
     1261 tccaaaattt aagtctacac ctataatatg agttcatatt agtactgcta aatcctatct
     1321 atagtaatgt caaacaccta aataaagtca tctctccaat tcattcaaga aatgaagaaa
     1381 ggcaaaaaat ctttaaaata agaaaaatgc atattatcag gtg
//
LOCUS       DAVGJJ010000152         1382 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000152 DAVGJJ010000000
VERSION     DAVGJJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.153"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            3..1337
                     /locus_tag="VVE35_001994"
     CDS             3..1337
                     /locus_tag="VVE35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU3062109.1"
                     /translation="MKLWDLILLGIGAMVGTGVFTITGTAAATLAGPALVISIVISAL
                     CVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAGWLTMMEFMTAISGVASGWA
                     AYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLVLVTSLVLLNAKAALRFNSI
                     LVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGASTGIMAGASLMFFGFLGFE
                     SISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLTGVVHYSHLNVDDAVAFALR
                     SVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLARDGLVPAAFKELTKTSKIPK
                     NATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAYGLIRLRKEKGMPKAGEFKT
                     PLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSIIYFTYGYKHSTIEE"
BASE COUNT          321 a          265 c          304 g          492 t
ORIGIN      
        1 atttgaagtt atgggatttg attttgctgg gtatcggagc catggtaggg acaggcgtct
       61 ttacaatcac aggtactgca gctgcaacac ttgctggccc agccctagtg atttcaatcg
      121 ttatttctgc cttgtgtgtg ggattatcag ccctcttttt tgcagaattt gcctcgcgag
      181 tacccgctac aggaggtgcc tatagttacc tctatgctat cttaggagaa ttccctgcct
      241 ggttggctgg ttggttaacc atgatggagt tcatgacagc catatcaggc gtagcttcgg
      301 gttgggcagc ttattttaaa ggcttgctct ctcaatacgg gatagccttt cctcaggctt
      361 taaatggtac ctttaatcct caagcaggga catttgttga tttattgcct attcttgtct
      421 tggtcttggt gacttcgctt gttttactaa atgccaaggc agccttacgc tttaattcga
      481 ttctagttat tttgaaattt tccgctttag ctctctttgt tttagtagga atttggaata
      541 ttaagtttga taattggagc aattttgctc cttacggttt tggacaaatc tatggagcta
      601 gtactggtat tatggctggt gcgtctttga tgttcttcgg ttttttggga tttgaatcca
      661 tctctatggc tgtagatgaa gtcaagactc ctcaaaaaaa tattcctaga gggattgtct
      721 tatcgctttc tatcgtaacc attctctatg ccttggtgac ccttgttttg actggtgtgg
      781 ttcactatag tcatttaaat gtcgatgatg ccgttgcctt tgcccttcgt agtgttggga
      841 ttagttgggc agccaactat gtgtcattag tggctatctt gaccttgatt acagtttgta
      901 tttcgatgac ctatgcccta tcgcgtatga tttacagttt agcacgtgac ggcttagtgc
      961 ctgctgcctt taaggaactg acgaagacta gcaagatacc aaagaatgct actatcttga
     1021 caggtctagc ttcagcagta gcagcaggaa tgttcccact agccagtatt gcagcctttt
     1081 taaatatttg taccttagcc tacttgatca tgttggctta tggtctgatt cgcttacgga
     1141 aagaaaaagg aatgcccaaa gctggggaat ttaaaacacc attggtacct ctgttaccaa
     1201 ttttatcgat catcatttgt ttatccttca tgttacagta taatatgaat acctggattg
     1261 ctttcctagt tgcgttgtta gtaggaagta ttatttactt cacttatggc tataagcatt
     1321 ctaccataga agaataaagt gaaagtctat tgagttgttg acactcagta gatttttata
     1381 tg
//
LOCUS       DAVGJJ010000153         1362 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000153 DAVGJJ010000000
VERSION     DAVGJJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.154"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            30..869
                     /locus_tag="VVE35_001995"
     CDS             30..869
                     /locus_tag="VVE35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU3062110.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            976..1251
                     /locus_tag="VVE35_001996"
     CDS             976..1251
                     /locus_tag="VVE35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU3062111.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          452 a          211 c          291 g          408 t
ORIGIN      
        1 ctgaatccta aaatagaaag attgatacta tgacaaaaat taagattgta accgattcat
       61 ctgttactat tgaacctgaa ctagtaaagc aattagatat tacaattgtt ccattatctg
      121 taatgattga taatgttgtt tattctgatg cggatttgaa agaagaaggt aaatttcttc
      181 agttgatgca agaaagtaag aatcttccga aaacaagtca gccacctgta ggtgtctttg
      241 ctgagatttt tgaagaccta tgcaaagatg gtggccagat tcttgctatt catatgtccc
      301 atgctctttc gggtacggta gaagcagcac gccaaggtgc tagcctatct actgcagatg
      361 tgactgttgt tgatagttcc ttcactgacc aagccctgaa attccaagtt gttgaggctg
      421 cgaagttagc tcaagaaggt aaagatatgg aggcaatttt atctcatgta gaagaggtta
      481 aaaaccatac agagctttat attggtgttt caactttgga aaatcttgtc aaaggtggac
      541 gaattagccg tgtaactggc ttgttgagct ctcttctcaa tatccgtgtt gtcatgcaaa
      601 tgaaagacca tgaattgcag ccaatggtta aaggtcgtgg aactaaaaca tttaaaaaat
      661 ggttagatga gttgataaca tcgctttctg aacgtgctgt agcagagatt ggaatttcat
      721 attctggtag tgatgattgg gcaaaagaga tgaaagaaag cttacaagct tatgttgaaa
      781 agccaatttc tgttttggaa acgggttcca ttattcaaac tcacacgggt gagaacgctt
      841 gggctatttt gatacgttac cactcctaaa aaaataaata aaatgggaag aaatagcgtt
      901 tttaacttga cctaaaaggg attttaggat atgattatat ttgttaatta gaaattattt
      961 ggaggaatca ttaacatggc aaacaaacaa gatttgatcg ctaaagtagc agaagctaca
     1021 gaattgacta agaaagactc agcagcagca gttgaagctg tatttgcagc agtagctgac
     1081 tatcttgcag ctggtgaaaa agttcagttg atcggttttg gtaactttga agttcgtgag
     1141 cgcgcagaac gtaaaggtcg caacccacaa actggtaaag aaatgacaat tgcagcttct
     1201 aaagtaccag cattcaaagc tggtaaagct cttaaagacg ctgttaaata atcagtcttt
     1261 aaaaagccta ttgtatcaag ctttctagct tggtcagtag gctttttaat tatgaaatct
     1321 agtaataaat agagttaaaa agttgaatgt tccgaaaata tt
//
LOCUS       DAVGJJ010000154         1340 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000154 DAVGJJ010000000
VERSION     DAVGJJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.155"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..620
                     /locus_tag="VVE35_001997"
     CDS             <1..620
                     /locus_tag="VVE35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4297 domain-containing protein"
                     /protein_id="HEU3062112.1"
                     /translation="EMQEQIDTEELFSSSWLLSDNQKKAVNNPKVDNFALVKTAFDNN
                     IINAKTFLKGEMANQRVSIDNRDDVIINELLQQIVQKSEKEVHTIADKELKKITSEEL
                     NLILQKVTAKARFDKELDKFGFTEIKNEKIKKEEKKIILEYITAKKSVVNFLNSDKNS
                     LENESVTTLIPKTFMLSEMGTLSENSRYAVGISAYCDILEGIANE"
     gene            613..>1340
                     /locus_tag="VVE35_001998"
     CDS             613..>1340
                     /locus_tag="VVE35_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEU3062113.1"
                     /translation="MSRMTIKSLLIADYENEKANKFVFSDSANLIVSSTNGEGKSSLV
                     KSIYYALGANLKSFPKGWNADNFIFQLEVFIDGNEFLIKRHNKVISVLDNNEVKLFEN
                     FAEYYLWFQEKLKMRLELVNKSSDKASLASVEALFSPMYIDQDKAWDGKLFKDSFESL
                     GRYKSNDFPKNVFDYYLGISDSQIVDKESKKNEYLRQLELVNGRINQVQSVYKTYRTK
                     NKITETVPKDFKDLQKELDFYITET"
BASE COUNT          542 a          149 c          239 g          410 t
ORIGIN      
        1 aagaaatgca agaacagata gatacagaag aacttttttc tagttcatgg ctgttatctg
       61 ataatcaaaa gaaggcagtt aataatccga aggtagataa ttttgctttg gtaaaaactg
      121 cttttgataa taacattatc aatgctaaaa catttttaaa gggagagatg gcaaatcaaa
      181 gggtttctat tgataataga gatgatgtaa ttattaacga attattgcag caaattgtcc
      241 aaaaatctga aaaagaagtg catacgattg cagataaaga actcaaaaag attacatcag
      301 aagagctaaa tttaatctta caaaaagtaa ctgcaaaggc acgatttgat aaagaacttg
      361 ataaatttgg ttttactgaa attaagaatg agaaaataaa gaaagaagaa aaaaagatta
      421 tattagaata tataactgct aaaaaatctg tcgttaattt tttgaacagt gataaaaata
      481 gtttagaaaa tgaatcggta acaacgttaa tacctaaaac atttatgtta tctgaaatgg
      541 gaacattatc agaaaacagt agatatgctg taggaatatc ggcgtattgc gatattttgg
      601 agggaattgc taatgagtag gatgaccata aaaagtcttt taatagcaga ttacgagaac
      661 gaaaaagcga acaaatttgt attttctgat tcagcaaacc taatagttag tagtacgaat
      721 ggtgaaggga aatctagtct agttaaatca atttactatg ccttaggagc aaacctcaaa
      781 tcatttccca agggttggaa tgcggacaat tttatttttc aattagaagt ttttattgat
      841 ggtaatgaat ttttgatcaa gagacataat aaagttattt ctgttttaga taataatgaa
      901 gtcaaactgt ttgaaaactt tgcagaatac tatttgtggt ttcaagagaa acttaaaatg
      961 aggctggaat tagtcaacaa aagcagtgat aaagcaagtt tagcttctgt tgaagccctt
     1021 tttagtccaa tgtatattga tcaggataaa gcatgggatg gtaaattatt taaagattca
     1081 tttgaaagtt taggacggta taaatcaaat gattttccca aaaatgtttt tgattattat
     1141 ctaggcatat ctgatagtca aatcgttgat aaagaaagta aaaaaaatga gtatttaagg
     1201 caattagaat tagtaaatgg aagaatcaat caggttcaat cagtatataa aacatatcgt
     1261 actaaaaata aaattactga gacagttcct aaagatttca aagacctaca aaaagaatta
     1321 gatttttata ttaccgaaac
//
LOCUS       DAVGJJ010000155         1324 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000155 DAVGJJ010000000
VERSION     DAVGJJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.156"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            292..759
                     /locus_tag="VVE35_001999"
                     /pseudo
     CDS             292..759
                     /locus_tag="VVE35_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            753..1283
                     /locus_tag="VVE35_002000"
     CDS             753..1283
                     /locus_tag="VVE35_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEU3062114.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKIEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
BASE COUNT          511 a          173 c          230 g          410 t
ORIGIN      
        1 atttgtattt gaaggtggtg ttcagataag aaatttagtc agacgaacca cgaatttgct
       61 ctatgctttc tggaatttat cataacagga ggatacagtc atggaacaaa cattgtttga
      121 attagaacta cttccagagg aagatgtcat tgtcacaggt ctccctaagt attgttcttt
      181 tacttgttta attacaggtc gctagttata ttttatataa aataagtagc tttacttacg
      241 gaataggcta gtgctgtgtc tctagcctat tttaataatt aggagtttgt tatggattta
      301 ttagagaaag aatgtttaaa atgtgataaa aatttccaac agggtgatat ttggaattac
      361 tattatttat cagataaggt gcctgcacaa gggtggaaaa tacacataag ctcccaaata
      421 aaagacgctg taaatatttt taagattgtg tataaactat cccaactaaa taattgtagc
      481 tttaaagttg ttaaaaattt agaggaatta aaaaaattaa ttcccctagg gaaatgagcc
      541 ctactgctaa caaatttata actctatatc ctaagtcaga atctgaagct aagagtatga
      601 tttgtaatct tacgaataaa ctgtcagaat ttaaggctcc aaaaatacta tctgactatc
      661 aatgtggaat gcattctcca gttcattata gatatggggc ttttttaaaa aaacaagctt
      721 atgatgaaaa aaataaaaaa gtcatctatt tattgctaga tgaaaaaagg aagaactatg
      781 tagaagataa gagacaaaat ttccctagtc ttcctagctg gaaaatggat ctattttcag
      841 aagaagaaaa aaggatttat tttcaaacca catgtgaagt ttcaagtaag gactctgcaa
      901 taaataaata caaaatagaa aaaattatta agagatcaaa taaaggaaat gtttatcggg
      961 caattagaaa atcggacggg caaaaagtaa tcatcaagca gtcaagacct tttgtgaatt
     1021 atgatgctga aggagaatgg acagctcttg atgatataaa aaatgaagct cacatgctaa
     1081 aaaaattagc cgataaaagt tatactacta atttaactga tgaattttat atcgtggatg
     1141 attattttct tgttcaggaa caagttgacg ggttaaactt tgaagaattt attcgagaaa
     1201 cagaacactc cttaaatatt agagaaaaaa cactggataa cattgtgaat atagtaagtg
     1261 acatccataa actgggaata taaaattgta gatatagctc ctacaaattt tatatatacc
     1321 aaaa
//
LOCUS       DAVGJJ010000156         1259 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000156 DAVGJJ010000000
VERSION     DAVGJJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.157"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>1259
                     /locus_tag="VVE35_002001"
     CDS             <1..>1259
                     /locus_tag="VVE35_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU3062115.1"
                     /translation="VEDLRLSYPYLPEGVKDLFTDMEKNKLFKPSPEIPENHPLNHYN
                     IECILKKSNRGNVYRAIEKSSNQKVIIKHARPYVYSWNDGFSAIDELKSEAANLKVFQ
                     DKSYTTDLIEEFYVNEDYFVVQKFIPGYNFFDLKNIDIEKNVIINNIIDIINDIHAEG
                     YLAVDISPTNFILDDFGRVRLIDLENFTNKNNTVRRAATKFMINTDCPSKICTIQQDY
                     FALAMLSFAIMREHVLIFEGEDVLLNITALDKIKFCMYSEKEIGNITKSQFNWLMYLL
                     NLSEQEKLDRFEKMKYINDFEDKNIEFHLKKVDSSFNLQIENETLINYLLSLNMNRES
                     RILNSNEFGEFVSPISFQHGLSGYLFYLSHMRDISENGRIIEWIISEGNQNKGNVYLN
                     GCSILFGSSGYLWVLLEFYKKITSNNP"
BASE COUNT          474 a          147 c          217 g          421 t
ORIGIN      
        1 tagtagaaga tctgagatta agttatccat atttgccaga aggggttaag gatttattta
       61 ctgatatgga aaaaaataaa ttatttaaac cctccccgga aatcccagag aatcaccctt
      121 taaatcacta taatatcgag tgtattctaa aaaaatccaa tagaggaaat gtttacagag
      181 ctatagaaaa aagtagtaat cagaaagtga taataaaaca cgcacggcca tatgtttata
      241 gttggaatga tggtttctct gcaattgatg aattaaagag tgaagcagca aatttaaaag
      301 tatttcagga taagtcctat acaacagatt taattgaaga gttttatgtc aatgaagatt
      361 acttcgtagt acagaagttt ataccaggat ataatttttt tgatttaaaa aatattgata
      421 tcgaaaaaaa tgtaatcatt aataatataa ttgatattat taatgatatt catgctgagg
      481 gatatttagc agtggatatt tctccaacaa actttatatt ggatgatttt ggtcgtgtta
      541 ggttaataga cttagaaaat tttacaaaca aaaacaatac tgttagaaga gctgctacaa
      601 aattcatgat aaatactgat tgtccctcaa aaatctgtac cattcaacag gattattttg
      661 cacttgcaat gttgagtttt gctattatgc gcgagcatgt tttaatattt gaaggagagg
      721 acgtcttatt aaatataact gctttagata aaatcaagtt ttgtatgtat agcgagaaag
      781 aaattgggaa cataactaaa tcgcaattta attggttgat gtatttattg aatttaagtg
      841 aacaagagaa gttagaccga ttcgagaaga tgaagtatat aaacgatttt gaagataaaa
      901 atatagaatt tcatttaaag aaagttgatt cctcattcaa tcttcaaatt gaaaatgaaa
      961 cgcttattaa ctatcttctt tcgttaaata tgaatagaga aagcaggata ctaaattcaa
     1021 atgaatttgg agaatttgtt agtccaatat cgtttcaaca tggcttatca ggatatttgt
     1081 tttatttatc acatatgaga gacatttctg agaatggtag aattatcgag tggattatct
     1141 ctgaaggaaa tcaaaataaa ggaaatgtgt atctaaatgg ttgttcaatt ttatttggtt
     1201 cttccggata tctttgggta ttattagagt tctataaaaa aattaccagc aacaatcca
//
LOCUS       DAVGJJ010000157         1255 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000157 DAVGJJ010000000
VERSION     DAVGJJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.158"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            123..629
                     /locus_tag="VVE35_002002"
     CDS             123..629
                     /locus_tag="VVE35_002002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062116.1"
                     /translation="MTLVIFPLIFLIVLVIFYLYPENVIINMLYSSYSSTLNLTDSSA
                     VKHLEDLWLPLLKVIDYPLGLGFGNNGPIVLSLYHSANLVESSIYLLAYDFGILGMFI
                     YLFPYFYTIFVYKKYLLSGAICCLVLITYLFLPNVENFEIIFFIYLFIGMDELALYTK
                     GMNVHEID"
     gene            616..>1255
                     /locus_tag="VVE35_002003"
     CDS             616..>1255
                     /locus_tag="VVE35_002003"
                     /inference="COORDINATES: protein motif:HMM:NF016357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU3062117.1"
                     /translation="MKLIKLLKKYINLYISSSVPKTNKLLIFGSWLGEKYADNPRYLF
                     EYVIKNRPDLKAIWITSNQDVFKELQKKQYLVMMAEAPETRKIVKKAKYIFTATGIFD
                     IGEQNANFVGGAYLINLWHGIPLKKIMYDDKHSALHKRSKLVTWVEKIPLRNYFVIST
                     STAITQIYQSAFRVKKSNILELGQPRNDYFYDKSNPVSSLIQELKSKNIILYM"
BASE COUNT          408 a          171 c          199 g          477 t
ORIGIN      
        1 tattgttttc aattgtagca gcttttgttc ttacatttag ccgtagtgct attataagta
       61 ctcttgtagg aatcgtattc tttcaacgta agaaattgtt ttcaacgacg aagattaatg
      121 ttatgacctt ggtaatcttc cccttaattt ttttgattgt tttagttata ttttatcttt
      181 atcctgagaa tgttattata aatatgctct atagcagtta ttcttcaaca cttaatttga
      241 cagattcttc agcggttaag catttggaag acttatggct tcctcttcta aaagttattg
      301 attatccttt gggacttgga tttggtaata atggcccaat tgttctgtca ctctatcact
      361 cagcaaattt agtagaatct tctatttatc ttcttgcata tgattttggt attttaggaa
      421 tgttcatata tttgttccct tatttttata cgatttttgt atataaaaaa tatttgctta
      481 gcggtgctat atgctgctta gttcttataa cttatctatt tcttccaaat gtagaaaatt
      541 ttgaaatcat attttttatt taccttttca ttgggatgga tgaactagca ctatatacaa
      601 aaggaatgaa tgtacatgaa attgattaaa ttattgaaaa aatatataaa tttatacatt
      661 agtagttcgg tgcctaagac aaataaattg ttaatttttg gatcatggtt aggtgaaaaa
      721 tatgcagaca atccaagata tttgtttgag tatgtgatta agaatcgtcc cgatttaaaa
      781 gctatatgga ttacaagtaa tcaagatgtt tttaaagaat tacagaagaa acaatatctc
      841 gtgatgatgg cagaggcacc tgaaactcgg aaaattgtta aaaaagcaaa gtatattttt
      901 acagcaactg gtatttttga tattggagaa caaaatgcta acttcgttgg aggtgcctat
      961 ctaattaatc tatggcatgg cattcctttg aaaaaaatca tgtatgatga taaacattca
     1021 gcacttcaca aacgtagtaa actagtgact tgggtagaaa aaattcctct tcgaaactat
     1081 tttgttattt ctactagtac ggcaataact caaatttatc agtctgcttt tcgcgtaaag
     1141 aaatctaata tattagaact tggtcaacct aggaatgact atttttatga taagtctaat
     1201 ccagtgagtt cattaataca ggaattaaaa agtaaaaata ttatcttata tatgc
//
LOCUS       DAVGJJ010000158         1206 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000158 DAVGJJ010000000
VERSION     DAVGJJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.159"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..237
                     /locus_tag="VVE35_002004"
                     /pseudo
     CDS             1..237
                     /locus_tag="VVE35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            392..604
                     /locus_tag="VVE35_002005"
     CDS             392..604
                     /locus_tag="VVE35_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062118.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATIDATKAVLLDAQKAPEDSALTE"
     gene            646..1050
                     /locus_tag="VVE35_002006"
     CDS             646..1050
                     /locus_tag="VVE35_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062119.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
BASE COUNT          401 a          243 c          267 g          295 t
ORIGIN      
        1 gatacttatt tttatcgaga atatggtcgc tcattaaaag gtcagttaat aagaggcaaa
       61 gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa tggtgagtta
      121 atcgctccaa tgacttacga agagacgatg acgagcgact tttttgaagc ttggtttcag
      181 aagtttctct taccaacatt aaccacacca tcagttatta tagtaaaacg aaataagaat
      241 aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa gtccatatgt
      301 actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc ctgtcccagt
      361 tccaagctca gctccgactc aagatcctag agtggtagta agcgcttcgg ctagctcaac
      421 gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga gtcaagagtt
      481 agaccaacgc ttgaaagctt tggcaacagt atctgatccg aaaatcgatg caaccatcga
      541 tgcaaccaag gctgtcctcc tagatgctca aaaagctccg gaagatagtg ccttgacaga
      601 gtaaggtctg catactgcag tagagtctgt gaaggctggg ctgccttgga ctctctcaaa
      661 gatgtgaaag caaatgctag cgatagcaag cctgcacagg acaagaagga tgcaaaacaa
      721 ggaacggaag atagtaagga ttcagataag atgactgaaa caaactcagt tccggcagga
      781 gtgattgtgg tcagtctact tgccctccta ggcgtgattg ccttctggct gattcgccgt
      841 aagaaagagt cagaaatcca gcaattaagc acggaattga tcaaggttct aggacagcta
      901 gatgcagaaa aagcggataa aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc
      961 cttgatttcg tgaaagaaga aaatggctca gcagagacag aaactaaact agtagaggag
     1021 cttaaagcaa tccttgacaa actcaagtaa aacatgattt atgaaatgtc agacctaaaa
     1081 gacattttcg ttcacaaaag cagcgatgag taatccttgt cgttgctttt tggctatttt
     1141 gggggataag atccgccttg actagcaaaa gaaaagattg gtccaatagt gcccgatacg
     1201 attttg
//
LOCUS       DAVGJJ010000159         1197 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000159 DAVGJJ010000000
VERSION     DAVGJJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.160"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            106..1023
                     /gene="adcAII"
                     /locus_tag="VVE35_002007"
     CDS             106..1023
                     /gene="adcAII"
                     /locus_tag="VVE35_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU3062120.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            1031..>1197
                     /locus_tag="VVE35_002008"
     CDS             1031..>1197
                     /locus_tag="VVE35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU3062121.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTP"
BASE COUNT          412 a          193 c          253 g          339 t
ORIGIN      
        1 tttatatgtg aaagaaattt caaatttttt ctaaaaatat cttgacagat taaattttca
       61 ggagtagaat atttattagt taattaaatg gttaaggagt tgttcatgaa gaaacaaaat
      121 ttatttttag tcctgttaag tgtctttctt ttgtgcttgg gggcttgtgg tcaaaaggaa
      181 agtcagacag gaaaggggat gaaaattgtg accagttttt atcctatcta cgctatggtt
      241 aaggaagtat ctggtgactt gaatgatgtt cggatgattc agtcaagtag tggtattcac
      301 tcctttgaac cttcggcaaa tgatatcgca gccatctatg atgcagatgt ctttgtttac
      361 cattctcata cactcgaatc ttgggcagga agtctggatc caaatctaaa aaaatccaaa
      421 gtgaaggtct tagaggcttc tgagggaatg accttggaac gtgtccctgg actagaggat
      481 gtggaagcag gggatggagt tgatgaaaaa acgctctatg accctcacac atggctagat
      541 cctgaaaaag ctggagaaga agcccaaatt atcgctgata aactttcaga ggtggatagt
      601 gagcataaag agacttatca aaaaaatgcg caagccttta tcaaaaaagc tcaggaattg
      661 actaagaaat tccaaccaaa atttgaaaaa gcgactcaga aaacatttgt aacacaacat
      721 acagcctttt cttatctagc gaagagattt gggcttaatc aacttggtat tgcaggtatc
      781 tctcctgaac aagaaccaag tccacgacaa ctaacagaaa ttcaggaatt tgttaagacc
      841 tataaggtta aaacgatttt tacagaaagt aatgcttctt caaaagtagc tgaaactctt
      901 gtcaaatcaa caggtgtggg tcttaaaact ctgaatcctt tagagtcaga cccacaaaat
      961 gacaagacct atttagaaaa tcttgaagaa aatatgagta ttctagcaga agaattaaag
     1021 tgaggaaaga atgaaaatta ataaaaaata tctagcaggt tcagtggcag tccttgccct
     1081 aagtgtttgt tcctatgaac ttggtcgtca ccaagctggt caggttaaga aagagtctaa
     1141 tcgagttgct tatatagatg gtgatcaggc tggtcaaaag gcagaaaact tgacacc
//
LOCUS       DAVGJJ010000160         1098 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000160 DAVGJJ010000000
VERSION     DAVGJJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.161"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..1079)
                     /locus_tag="VVE35_002009"
     CDS             complement(<1..1079)
                     /locus_tag="VVE35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU3062122.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILG"
BASE COUNT          390 a          189 c          136 g          383 t
ORIGIN      
        1 cctaaaatta aatacttttc attacgttga aaacaaaaat ttaagttttt taatatttct
       61 ttatctccat acttatagca aatatttttt gcttcatata acggaaaatc tctattcacc
      121 tcatttggtt cgatatcatt cattttattt gactcaattg gattaattga atacaatttt
      181 aaaaaaatag gcttcgtacc aataatagag gataattgac ctcctaattc acctagcgct
      241 gtaaaaataa cacctgttag tgctcctatt gcttcaatag taccaatttt cactattcct
      301 tttattgcaa gatagcctgt taaaaaaacg agagatatct gaaaaaaaat attgagaaag
      361 aagctaatag cgcctgctaa cgtttctaca gttgtctttc tttgtataac catctttaat
      421 aaaattcctg cttctttaat tttcttaggc aatacatata aaagattcaa ggacgctaac
      481 acatcaaatc cattcaatat agtctcacta gattttaaaa aagcttcatt ttggttagtt
      541 aaatttagac taacttctcg cattttcgat gcaaagattt ttggtacaag tagcataatc
      601 attaatgaaa acaaggtggc tacagtcaat gaccaatgat agtgattaag agtcacaact
      661 gcaaatatag taccagaaat tccttttatt actaaaaaaa gttgtttaaa agcctgatca
      721 tttaaagtct gaacatcatt atttagccac gaaagatatg ttcctgatga tttactatga
      781 aattcttgat aggtagagtt agagatgtct gtggcaactc tatttcgaat ctctagatta
      841 aactcttgga tcacttcaac ctgataattt ttcactaccc agtcaaggaa tattatccca
      901 caccagacaa tcatttggta gattgacaat ttcaaaaacc gctctaaatt catcgcaatt
      961 aattcattca acaccagagc attaatagtt gctgcataaa ttagcaataa ttgaccagca
     1021 acaataaata tcgttaataa actaaatttt tttatatttg attttataat agtatacaca
     1081 atagtttctc actttcta
//
LOCUS       DAVGJJ010000161         1074 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000161 DAVGJJ010000000
VERSION     DAVGJJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.162"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..1014
                     /locus_tag="VVE35_002010"
     CDS             <1..1014
                     /locus_tag="VVE35_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEU3062123.1"
                     /translation="TSKTSDIEKKSTQEAVLNKNFRQAINFAFDRTSYGAQSEGKEGA
                     TKILRNLVVPPNFVSIKGKDFGEVVASKMVNYGKEWQGINFADGQDPYYNPEKAKAKF
                     AEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGVSSIKQSIESVLGSDNVVIDIQQLTS
                     DEFGSSGYFAQTAAQKDYDLYHGGWGPDYQDPSTYLDIFNTNSGGFLQNLGLEPGEAN
                     DKAKAVGLDVYTQMLEEANKEQDPAKRYEKYADIQAWLIDSSLVLPSVSRGGTPSLRR
                     TVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDEYAKAREKWLKEKEESNKKVQEELAK
                     HVK"
BASE COUNT          374 a          178 c          237 g          285 t
ORIGIN      
        1 acttctaaaa caagtgacat tgaaaagaaa tcgactcagg aagcagttct caataaaaac
       61 ttccgtcagg ctatcaattt tgcttttgac agaacatctt atggggctca gtctgaaggg
      121 aaagaaggtg caacaaagat tttgcgtaac ctagtggttc ctccaaactt tgtcagtatc
      181 aagggaaaag actttggtga agttgtagcc tctaagatgg tcaactatgg taaggaatgg
      241 caaggtatca actttgcgga tggtcaagac ccttactaca atcctgagaa agccaaggct
      301 aagtttgcgg aagctaagaa agaactcgaa gcaaagggtg ttcaattccc aatccacttg
      361 gataagactg tggaagtaac agataaagta ggcatacaag gagttagttc tatcaaacaa
      421 tcaattgaat ctgttttagg ttctgataat gtagtgattg acattcagca attaacatca
      481 gatgagtttg gcagttcagg ctactttgct caaacagctg ctcagaaaga ttatgattta
      541 tatcatggcg gttggggacc tgattatcaa gacccgtcaa cctatctcga tatttttaat
      601 actaatagtg gaggatttct gcaaaatctt ggactagagc ctggtgaggc caatgacaag
      661 gctaaggcag ttggactgga tgtctatact caaatgttgg aagaagctaa taaagagcaa
      721 gatccagcca aacgttatga gaaatatgct gatattcaag cttggttgat tgatagttct
      781 ttagttcttc caagtgtttc gcgtggggga acaccatcat tgagaagaac cgtaccattt
      841 gctgctgcct atggtttaac cggtacaaaa ggggttgaat catataaata cctcaaagta
      901 caagataaga ttgtcacaac agacgaatat gcaaaagcca gagaaaaatg gttgaaagaa
      961 aaagaagaat ccaataaaaa agtccaagaa gaattggcaa aacatgtcaa ataaaatcta
     1021 attgtagata agtttgtgta agaagtcttt gtgcttaaaa acgcataata tcag
//
LOCUS       DAVGJJ010000162         1072 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000162 DAVGJJ010000000
VERSION     DAVGJJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.163"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..143)
                     /locus_tag="VVE35_002011"
     CDS             complement(<1..143)
                     /locus_tag="VVE35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU3062124.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGL"
     gene            complement(155..721)
                     /gene="amaP"
                     /locus_tag="VVE35_002012"
     CDS             complement(155..721)
                     /gene="amaP"
                     /locus_tag="VVE35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU3062125.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(805..969)
                     /locus_tag="VVE35_002013"
     CDS             complement(805..969)
                     /locus_tag="VVE35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU3062126.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          362 a          214 c          152 g          344 t
ORIGIN      
        1 tataatccag ctgcaactcc cagtgctcct aaaatcaata caaatattgt tttgaagaag
       61 ccaaaggaga caatcaaaca agccagaaat acgcctatga gaccagcgat aattggatat
      121 cgatattgtt taagccattc cattttttac ttccttactt cacacgacta acagtctttt
      181 tgttttgagg ttttggttgg taatttttta ctgcaacttc aagttttact tgacgctcaa
      241 taccaaaaaa ctgcttcaat ccattagtta tttcattttg aattatttgg catctgtcag
      301 cgatgttgtc tgaaggaaga attttacctt ctacatgaac gaaacattta ttttttcgta
      361 aatttacatg aacagttggg ttcttgatca atctatgatc actcaccaaa cttctaacaa
      421 aaccttcgat tgccgaattc tttaatttta atgtatcgtt tttagtttca agttggattt
      481 ccaagtaacg tttaggataa aacatcacaa ctaaaatgga taataaaact aaagttgata
      541 gaaccactgt cccccaaaag acatatctag caagatagaa ttcaactacg gagttctgtc
      601 tccagctaag tagatgaata cctagatcac taacttgatg ataatctatc aaaacaggaa
      661 ggaaaattgt caagattaaa atacagaaaa taatgaaaca tattttcttt gcttttgaca
      721 tatacaatcc tttcgattta acatttaaac tatttttccc gtcattgatt taaaaatagt
      781 ttgctaactt tactggcgaa aagtttactt tctacctgta aagaatgaca ctacagtaat
      841 cacaatcgct gcacctacaa tagatgggag caaagccatt ccagcgattg atggacccca
      901 actacctaaa agagattgtc ctgcatatgc cccgactaaa ccagcgaata catttgcgat
      961 gattcccata gaactacttt tttagtgatt ttacctgcca agagaccaat aagacctcca
     1021 gcaataattg atcacaacac tgcctaccct ccttttttat tttaatatca aa
//
LOCUS       DAVGJJ010000163         1052 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000163 DAVGJJ010000000
VERSION     DAVGJJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.164"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(28..1020)
                     /locus_tag="VVE35_002014"
     CDS             complement(28..1020)
                     /locus_tag="VVE35_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3062127.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     ILNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          345 a          174 c          130 g          403 t
ORIGIN      
        1 taaaaacatg gcttgtaaaa aattacttta atcacaataa tcgcatttaa aattgtgatg
       61 tttgcaagct aaattacgga cttcacttgg aagttttccc ttgtatcttt tataatagat
      121 agaaaatttg ctggcagatg aatatccaac agattctgct atctctttta taggtagttc
      181 agtgtttaaa ataagagttt cagctacatt cattcttttt ctttgagtgt actctgtaat
      241 gctttgacaa tatttttcct taaataaatt ttttaattta gtaccactca ttttagatat
      301 tttttcaagc gtgccttgat ttacattcgt tgcaaaatga tcatctaaga atcttgctac
      361 atcttcaagt gctttatcat catcaatttc aattttatat ttttttctat ttaagtatgt
      421 gtcaattact atacttatcc attcatttgc ctttgcttta aagaaaaaat cagcggcagg
      481 agcgtccatc ttacaattta atatttccat tgccactctt tctaaggcct ttgtaagtat
      541 tatttgattc ggttgaagca aggttgaata aaaagattct ggattaatgt taatagatgc
      601 taaatgtttt tctattagct cttttttaaa accaatggaa acagcaagat aacaacaatt
      661 ctcgtgtaat aaaaaaacaa aatcatcttt tatattatca aaatcaaaag tacataaaga
      721 gtttgcggta atagtttgat acggattaaa cttttctccg tttgcactga caatgtaact
      781 tgaataaatt gaaacatagt ctgacatact ataagtgcta ttttgaacta cttcctcttt
      841 gatataaaaa tcatgtatat cgataatgaa gctgcctcct tcataaaacc aatataggcc
      901 ttctgcataa gttgaattct cttttttcca acaaaatgta tgacctgcac ttgaatactt
      961 ctcattgttt ttacattcat ctactttcat ataatctttt acaaaatcat aatatgacat
     1021 aacacactat cccttttaga caatattcca at
//
LOCUS       DAVGJJ010000164         1032 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000164 DAVGJJ010000000
VERSION     DAVGJJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.165"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..480
                     /gene="rlmH"
                     /locus_tag="VVE35_002015"
     CDS             1..480
                     /gene="rlmH"
                     /locus_tag="VVE35_002015"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU3062128.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            524..597
                     /locus_tag="VVE35_002016"
     tRNA            524..597
                     /locus_tag="VVE35_002016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:558..560,aa:Arg,seq:tct)
     gene            762..887
                     /gene="comC"
                     /locus_tag="VVE35_002017"
     CDS             762..887
                     /gene="comC"
                     /locus_tag="VVE35_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU3062129.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            908..>1032
                     /locus_tag="VVE35_002018"
     CDS             908..>1032
                     /locus_tag="VVE35_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComD"
                     /protein_id="HEU3062130.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLL"
BASE COUNT          363 a          128 c          201 g          340 t
ORIGIN      
        1 atgaaaatta aagttgtaac agttgggaaa ctgaaagaaa agtatttaaa agatggtatc
       61 gcagagtatt caaaacgaat ttctagattt gctaagtttg aaatgattga gttatcagat
      121 gaaaaaacac cagataaggc cagtgaatca gaaaatcaaa agattttaga aatagaaggt
      181 cagagaattt tatcaaaaat tgctgaccgt gatttcgtta ttgtgttagc cattgaaggg
      241 aaaactttct tctcagaaga atttagtaag cagttagaag aaacttctat aaaaggattt
      301 tctactctta cttttattat tgggggaagt ttaggattgt catcatctgt aaaaaataga
      361 gccaatcttt ctgtcagttt tggtcgccta accttacccc atcagttaat gagactagtt
      421 cttgttgaac aaatctatcg cgcttttacg attcagcagg gattccccta ccataaatag
      481 aaaattgact tttaattgaa tttttggtag aataattgta ttaggtctca tagctcagct
      541 ggatagagca ttcgccttct aagcgaacgg tcgcaggttc gaatcctgct gggatcaata
      601 taatagcaaa gctgggaatt ttcccggctt ttttcttaaa aaagtacact ttgggagaaa
      661 aaaatgacag ttgagagaat tttatctaaa acgaaattcc attttgtata atggtttttg
      721 taagttagct tacaagaaaa aacattttag gagattttat tatgaaaaac acagttaaat
      781 tggaacagtt tgtagctttg aaggaaaaag acttacaaaa gattaaaggt ggggagatga
      841 ggttgtcaaa attcttccgt gattttattt tacaaagaaa aaagtaatga aataagggga
      901 aagagtaatg gatttatttg gatttgggac ggttattgtt cattttttaa ttattagtca
      961 cagttaccat tttatttgta aaggtcaaat aaatagaaaa gaattattcg tttttggtgc
     1021 ttatacatta ct
//
LOCUS       DAVGJJ010000165         1016 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000165 DAVGJJ010000000
VERSION     DAVGJJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.166"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            1..1016
                     /locus_tag="VVE35_002019"
                     /pseudo
     CDS             1..1016
                     /locus_tag="VVE35_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          377 a          180 c          205 g          254 t
ORIGIN      
        1 tcctgattta aaacaagaaa tgattcataa agtctgacat gaaggctgga ctaaagatag
       61 agtttctctt gaatacggtc tcccaagtcg tacgatactt cttaactggc tagcacaata
      121 cagaaaaaaa cgggtatact attgttgaga aaacaagagg gagagtacct aaaatgggac
      181 gtaagccaca aaagagacct gaagagagga cagaattaga acgtcttcaa gcagaaaatg
      241 agtacctgag agcggagaat gccatcctaa aaaagttaag agaactccga ttgaaggagg
      301 aaaaagagaa agaagaaaga cagaaattgt tcaagaatta atgactgagt ttttgttagt
      361 tcttcttcta aaatccatta aactagctcg ttggacctac tactatcact tgaaacagct
      421 agacaaaaca gataaggacc aagagcttaa agctgaaatt caatccatct ttatcgaaca
      481 caaaggaaat tatgcttatc gccgggttca tttagaacta agaaatcgtg cttatctggt
      541 aaatcataaa agagttcaag gcttgataaa agtactcaat ttacaagcta aaatgcgaca
      601 gaaacgaaaa tattcttctc ataaaggaga cgttggcaag aaggcagaga atctcattca
      661 aggccaattt gaaggctcta aaacaatgga aaagtgctac acagatgtga ctgaatttgc
      721 cattccagca agtactcaaa agctttactt accaccagtt ttagatggct ttaacagtga
      781 aattattgct tttaatcttt cttgttcgcc taatttagaa caagtacaaa caatgttgga
      841 acaggcattc aaagagaagc actacgagaa tacgattctc catagtgacc aaggctggca
      901 atatcaacac gattcttatc atcggttcct agagagtaag ggaattcaag catccatgtc
      961 acgtaagggt aacagcccag acaatggtat gatggagtcc ttctttggga ttctga
//
LOCUS       DAVGJJ010000166         1000 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000166 DAVGJJ010000000
VERSION     DAVGJJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.167"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(53..136)
                     /locus_tag="VVE35_002020"
     tRNA            complement(53..136)
                     /locus_tag="VVE35_002020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Leu,seq:caa)
     gene            complement(146..217)
                     /locus_tag="VVE35_002021"
     tRNA            complement(146..217)
                     /locus_tag="VVE35_002021"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(183..185),aa:Gln,seq:ttg)
     gene            complement(228..300)
                     /locus_tag="VVE35_002022"
     tRNA            complement(228..300)
                     /locus_tag="VVE35_002022"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:His,seq:gtg)
     gene            complement(315..385)
                     /locus_tag="VVE35_002023"
     tRNA            complement(315..385)
                     /locus_tag="VVE35_002023"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Trp,seq:cca)
     gene            complement(390..470)
                     /locus_tag="VVE35_002024"
     tRNA            complement(390..470)
                     /locus_tag="VVE35_002024"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Tyr,seq:gta)
     gene            complement(483..555)
                     /locus_tag="VVE35_002025"
     tRNA            complement(483..555)
                     /locus_tag="VVE35_002025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Phe,seq:gaa)
     gene            complement(559..632)
                     /locus_tag="VVE35_002026"
     tRNA            complement(559..632)
                     /locus_tag="VVE35_002026"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Met,seq:cat)
     gene            complement(646..735)
                     /locus_tag="VVE35_002027"
     tRNA            complement(646..735)
                     /locus_tag="VVE35_002027"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Ser,seq:tga)
     gene            complement(818..891)
                     /locus_tag="VVE35_002028"
     tRNA            complement(818..891)
                     /locus_tag="VVE35_002028"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(855..857),aa:Ile,seq:gat)
     gene            complement(926..996)
                     /locus_tag="VVE35_002029"
     tRNA            complement(926..996)
                     /locus_tag="VVE35_002029"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Gly,seq:tcc)
BASE COUNT          289 a          270 c          228 g          213 t
ORIGIN      
        1 aaaataatat aaaaaaggaa tgaagaataa caacgttcct aacactatac tataccggcg
       61 gccggggtcg aaccggcacg tccttgcgga cactggattt tgagtccagc gcgtctgcca
      121 attccgccac gccggcaaat agtaactggg gtagctggat tcgaaccaac gcatgaggga
      181 gtcaaagtcc cttgccttac cgcttggcta taccccaata taaaataggc gagtgatggg
      241 gatcgaaccc acgcatgcca gagccacaat ctggtgtgtt aaccacttca ccacacccgc
      301 cataattcta ttaacacggg cagtaggaat tgaacccaca ctgaaggttt tggagacctt
      361 agttctacct ttaaactatg cccgtaaaat ggaaggggag ggattcgaac ccccgaaccc
      421 gaaggagcgg atttacagtc cgccgcgttt agcctcttcg ctacccttcc aaaatatata
      481 aatggcgcga gacggaatcg aaccgccgac acatggagct tcaatccatt gctctaccaa
      541 ctgagctacc gagccttatt gcgggagcag gatttgaacc tacgaccttc gggttatgag
      601 cccgacgagc taccgagctg ctccatcccg cgttaataat ataaaaggag gatgtgggat
      661 tcgaacccac gcacgttttt acacgcctga cggttttcaa gaccgttccc ttcagccgga
      721 cttgggtaat cctccaaaat agtttatacg ggaacaagcc cgtgtcctct tagtgaacag
      781 ataatattcc aatcccctag taatggacta aacactatgg gcacgagtgg actcgaacca
      841 ccgacctcac gcttatcagg cgtgcgctct aaccacctga gctacgcgcc caagtcaaaa
      901 acttggtaca aagaacaaag ttcaaagcgg gtgacgagaa tcgaactcgc gacaacagct
      961 tggaaggctg tagttttacc actaaactac acccgctaaa
//
LOCUS       DAVGJJ010000167          931 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000167 DAVGJJ010000000
VERSION     DAVGJJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.168"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..722
                     /locus_tag="VVE35_002030"
     CDS             <1..722
                     /locus_tag="VVE35_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU3062131.1"
                     /translation="KYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTIKKA
                     LDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHF
                     KLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKS
                     IYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEY
                     SISRHRYDLFEFNSFALRHSS"
BASE COUNT          307 a          151 c          169 g          304 t
ORIGIN      
        1 caaaatattt atttatttac aatgagattc gtgaaaagat tctttgtaat aaatatacca
       61 tgaacgaaca attgcctgat gaaatgacat tagctaaaca gtttgcctgt agtcgaatga
      121 cgatcaaaaa agctttagac ttgttagttt ctgagggctt aatttttaga aaacgtgggc
      181 agggaacctt tgttctctct cgtggcagct caaaaagaaa attaatcgtt ccagaaagag
      241 atatccgggg actgacaaaa atatctgaag atgctcattc tacaattgac tcgaggatta
      301 ttcacttcaa attagaattt gcaaatgaat ttttagcaga aaaactacag gtcgctttgc
      361 agagtccagt ttataatatt taccgtctgc gtattattga cggtaaacct tatgttctgg
      421 aacaaactta tatgagtact gatgttattc caggtattac tgaagatatt ttacaaaaat
      481 cgatttacaa ttacattgaa ggaaagttag gattgcatat tgccagtgct acaaaaatct
      541 tgcgagcttc ttctagttca gaaaatgagc aacattactt gcagctcctt ccaacggaac
      601 cggtatttga agtagaacaa gtggcttatt tggataacgg aactccgttt gagtactcga
      661 ttagtcgtca tcgctatgat ttatttgaat ttaattcttt tgcattacga cattcctcct
      721 aggagaaaat gtgaaaatga agccaatctt ttacagactc tagtttaaga aaaatttaaa
      781 acagggcaag aaggtcccat ctatgcttaa atggtttctc ttttctaaat aagatggctt
      841 taaaagagtg atcgttttat ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta
      901 ggtactgaaa ttgttcacct gatctacttc t
//
LOCUS       DAVGJJ010000168          837 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000168 DAVGJJ010000000
VERSION     DAVGJJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.169"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..149)
                     /locus_tag="VVE35_002031"
     CDS             complement(<1..149)
                     /locus_tag="VVE35_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3062132.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFE"
     gene            complement(162..>837)
                     /locus_tag="VVE35_002032"
     CDS             complement(162..>837)
                     /locus_tag="VVE35_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU3062133.1"
                     /translation="NWIFSNYQITDEVKVLELGCGTGELWKSNSDSIDKMKQLIVTDF
                     SKDMVKSTKSVIGNRNNVNYEIMDIQKISFENETFDIVIANMLLHHVNDIPKALSEVN
                     RVLKTGGIFYCATFGENGVVNYLASLFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDT
                     LLYDDELQVTSIDDLVKYIQSFKGISEIGSLEEEIIRKRLESEFSNGMLIIPKEYGMF
                     IARKES"
BASE COUNT          280 a          168 c           86 g          303 t
ORIGIN      
        1 aattcaaaaa atgttgaaag agaaatttca ttttctcccc tttcaaatct agaaagttga
       61 gattttgata taaaatcagc tgcagcatac tctaaagaat atccgtttaa ttttctaaaa
      121 tatcgaaaag cttctccgta atttttcaca ctttcattcc cttaactttc ttttcgagcg
      181 ataaacatac catattcttt aggaatgatc aacataccat tactaaactc actttccaat
      241 cttttacgta ttatttcctc ttctagtgaa cctatttctg aaattccttt aaacgattgg
      301 atgtatttaa ctaggtcatc tatactagtt acctgtaact catcatcata gagtaatgta
      361 tcgacagagt taaaatacct actcaggtac cttttaccat tttgtaaagt aaacgttcta
      421 ttttctaaat cttgattaac ctcatcttta aataaacttg ccaaataatt tacaactcca
      481 ttttcaccaa atgtagcaca gtaaaaaatg cctccggttt ttaagaccct attcacctca
      541 gagagagctt taggaatatc attcacatga tgtagaagca tattagcaat aacaatatca
      601 aaagtttcat tctcaaaaga aatcttttga atatccatta tctcataatt gacattgttt
      661 ctatttccaa tcactgactt cgttgatttt accatatctt tagaaaaatc tgtaacaatc
      721 aactgcttca ttttatctat agaatcacta ttacttttcc acaattctcc tgtaccacat
      781 cccaattcta gaacctttac ttcatcagtg atttggtagt tagaaaatat ccaatta
//
LOCUS       DAVGJJ010000169          814 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000169 DAVGJJ010000000
VERSION     DAVGJJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.170"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            310..561
                     /locus_tag="VVE35_002033"
                     /pseudo
     CDS             310..561
                     /locus_tag="VVE35_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          270 a          147 c          130 g          267 t
ORIGIN      
        1 gttggaatta gtgcatcaaa aattaaggta tgagtcggag taacaacaac taaaagtaaa
       61 attataacaa ataaaaaaga atttaatttc aagttcaata ttaatttact cacctatttc
      121 tccttatatt atatttagtg tagttcgcta ctgctaaagt cactgtggat tagttcattt
      181 cctccaatcg cttgacgatt tctgaaaggc tctaaccaca gctttttgtt tcaattggta
      241 atgagtttga tagcgcataa acgttttata ttatgtaagg taacaaggca caaagttcgt
      301 ggataaccaa gtgagcttgg gaatcgttta ggtgacgtta tcttagttga gattcgaagt
      361 attcatatct tcgataagcc tcatcaatta caagcttttg ctggaccgaa tccttctatt
      421 taccagtcag gtcaaatcga tttagcaggg aaaatggtca aactgggttc ccctcatcta
      481 cgttgggcac tcatacaagc taccaaagca tatgctcgct tttcgcctgt ttttaaagcc
      541 tatcttagga ctaagttgta acaaagaaag cattacaatg tagccaccat tcaccttgcc
      601 aaaaaactta ttagaaatct gttttatctc ctaaaaaaca agctaccatt tgacgaaaaa
      661 aagtgttcta actcctgaat ttgccacaag ataacctttg aaaaaataaa ctttttcaag
      721 gacattttgc cgtgctctaa aatcatccac tttgaaacaa gaagtatttc agattaaact
      781 attgaatttt cttatagtta gtctttgtat ataa
//
LOCUS       DAVGJJ010000170          788 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000170 DAVGJJ010000000
VERSION     DAVGJJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.171"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..318
                     /locus_tag="VVE35_002034"
     CDS             <1..318
                     /locus_tag="VVE35_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU3062134.1"
                     /translation="QSLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLARGLI
                     RNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQFPK
                     DFI"
BASE COUNT          279 a           97 c          144 g          268 t
ORIGIN      
        1 cagtctttag agcaagttgg tttgagtgta aaagattttc ctaataacat tttagattat
       61 tatgttggtg atgatgggag attactgtca ggagggcaga aacaaaaaat tactttagct
      121 agagggctaa ttagaaataa gaaaatagta ttaattgacg agggaacttc tgctatcgat
      181 aggagaactt cgttagcgat tgaacgtaag atattagata gagaggattt gactgtcatt
      241 attgttaccc atgctccgca tccggaactt aaacaatatt ttactaagat atatcaattt
      301 ccaaaggatt ttatttaaga atatagggga gggaaacatg agattctatt gtgaagctta
      361 tgaggtaatt actgaagaga aaaaagtatc atttttttat gtagatgaga aacccttaag
      421 gtatctgtta tttgactata tatttctact agttctagct aaaaaaaata tcccaatctt
      481 atgaactgca ccccaaaagt tagatttatt ctgtctaact tttggggtgc agttcatttt
      541 gcatagtttt ggcattcatc ctagatatcc atataaaatg tctgagattg ttgaaatgtc
      601 taaaaaaata tcagatttaa acgataaaaa agaatttcac aaaaagaact gtgagttgag
      661 gtttcaagat agtgttcttg ataacataaa tagtgtcccg ggtacaactc atttatttag
      721 ttgtttaaat attcaaacat tagtaaaaat tattttatat atttttcgat acaacagttg
      781 tatcgaaa
//
LOCUS       DAVGJJ010000171          736 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000171 DAVGJJ010000000
VERSION     DAVGJJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.173"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..>736)
                     /locus_tag="VVE35_002035"
     CDS             complement(<1..>736)
                     /locus_tag="VVE35_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU3062135.1"
                     /translation="ELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSA
                     MVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELE
                     PSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYL
                     AKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQ
                     KAKETQLIGGQDNFDEAWDSYLEYINV"
BASE COUNT          239 a          139 c          106 g          252 t
ORIGIN      
        1 caacatttat atattctaag taagaatccc aagcctcatc aaaattatct tgtcctccaa
       61 tcaattgggt ttcttttgct ttctgacaaa aatctttaaa aataccgtcc tgtagcctaa
      121 actcaatagc tccatcccca tttgatactg gtcttaaacc ttctacaaaa tccgtataat
      181 catatgatgg gtgaaattgt acaaatccga tttggtcttc gttgccatcc gttaattctt
      241 tggcaatttc tttagcaaga taagtctttc ctgtgccagg agcaccacga aggataaggt
      301 ttggagactt taatagagat tgagttaatt gatgacgaaa attgtttgat gaatccattg
      361 tactattgtc ttccttattt tccataaaaa gcttagaata ctcaaaaagc atagttccta
      421 atttcgatgg ctccaactca ggataagttt tggacaccag ttcaattatt ttatgatttt
      481 gcagataagg tccaccttga tttgtctgag aaacaaagtg gttccaaaat tctttctgac
      541 tattaatatt tacaccaaag aaaggatctc cctcagaata gatacaaagt aattttgtca
      601 ccatagcaga acgaccaata aattcatttg ttgatctttt catatcaaaa ataggatttt
      661 caaagttaaa acgaatacct tctttgataa tttcatacaa atctgatttt aattttgaaa
      721 atcgctgatc caactc
//
LOCUS       DAVGJJ010000172          727 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000172 DAVGJJ010000000
VERSION     DAVGJJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.174"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..648
                     /locus_tag="VVE35_002036"
     CDS             <1..648
                     /locus_tag="VVE35_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU3062136.1"
                     /translation="IVAINNGINVSNSDLDVVGVQDFKKEFCIPNNKKIICYVGRLDP
                     EKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPAT
                     IVYQISDLLYIPSDTEGIPMCVLESMSQGTLVLASNVGGLSEIIEHRVDGFLFEKEDV
                     EGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG"
     gene            641..>727
                     /locus_tag="VVE35_002037"
     CDS             641..>727
                     /locus_tag="VVE35_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3062137.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKS"
BASE COUNT          260 a           67 c          157 g          243 t
ORIGIN      
        1 atagttgcaa taaataacgg aattaatgta tcaaatagcg atttagatgt agtaggtgtt
       61 caagacttta aaaaagaatt ttgtattcca aataacaaaa aaatcatttg ttatgttgga
      121 aggttggatc cagaaaaaag gccggataga ttccttgaat ttgcagaaaa attattttta
      181 gtcagagaag atgtgatttt tattatggct ggaaatggta gtatgtgggc tgctttgaaa
      241 gaaaaaattt gccatttgaa atgtagagat aatttcaggt tacttggtga aatctatcca
      301 gctactatag tttaccaaat atcggattta ttgtatattc cttctgatac agaaggaata
      361 cctatgtgtg ttttagagtc aatgtcacaa ggaactctgg ttttagctag taatgttggc
      421 gggttaagtg aaattattga acatagggtt gatggatttt tatttgagaa ggaagatgtt
      481 gagggagtgt gtgcttgtgc taatttttta ctcaatgatt ctgagtattt gaaatatata
      541 ggtgagaata gtaaatcaaa aataagaaaa catttttctg tgcaaaaaat gtttgtagaa
      601 accatgagag tatatgatga attattagag aagagtagtc atggatagga atatagatca
      661 agaattagta agtattataa taccaacaca taataggtat gaatctctga ttagagctgt
      721 gaaaagt
//
LOCUS       DAVGJJ010000173          722 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000173 DAVGJJ010000000
VERSION     DAVGJJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.175"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..478
                     /locus_tag="VVE35_002038"
     CDS             <1..478
                     /locus_tag="VVE35_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU3062138.1"
                     /translation="DLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLK
                     HNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPL
                     FYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(426..>722)
                     /locus_tag="VVE35_002039"
     CDS             complement(426..>722)
                     /locus_tag="VVE35_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU3062139.1"
                     /translation="SFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLP
                     LISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          251 a          107 c          110 g          254 t
ORIGIN      
        1 cgatttaagc attaataata aaggtgttag aattatacgt ggtggtaata ttaagccttt
       61 agaattttct ctgttggata acgattacta cattgataca caattcatct cctctgagca
      121 agtttattta aaacataatc agctaataac acctgtatca acctctttag aacatattgg
      181 aaagtttgca agaatcgata aagactatga tggtgttgtg gctggtggat ttattttcca
      241 attaacacca ttcgaaagtt cagagattat ttcaaaattt ctattattta acttgtcctc
      301 tccgttattt tataaacaat tgaaagcaat aactaaacta tcaggtcaag ctttatataa
      361 tattcctaaa actacactaa gcgagctatt aattccgtta gctccttttg aggaacagga
      421 acttattact caaaaagttg agaaactttt tgaaaaagta aatcaacttt ggaaatgatt
      481 cttttcatct cttcatgatt agaaataggg attaataatt cggagatact ggtactattt
      541 aatgttttcc ctttgatagc atcttttgaa tcacctaaag tagagataag tggcaaaaat
      601 atcattaagt aatctctgat aatattttct ttattagcat aggggaatat cgatataatg
      661 gcttcattat gagtggcagg aatatccaat atggcaactt ttccaataga taatttaaaa
      721 ct
//
LOCUS       DAVGJJ010000174          674 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000174 DAVGJJ010000000
VERSION     DAVGJJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.176"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            134..>674
                     /locus_tag="VVE35_002040"
     CDS             134..>674
                     /locus_tag="VVE35_002040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062140.1"
                     /translation="MKLNEVRVKDFNFGDVDGSDESLEPKFQDLFYDEDSFYEKELSK
                     KKKFIIYGRKGTGKTLLASYLNEKMRRNANQSSKIVSYDEYIYEKLKEFKYDSVKKEE
                     QSLFWRYVFLQQFVDLIKKDYEYARFWQFYRKRKIKKLIESVTRQFLEVQQFINEDST
                     EIKNSGKFNSKISDLGAELQ"
BASE COUNT          259 a           64 c          131 g          220 t
ORIGIN      
        1 taagtagaat gtagttacat tctaagaatt aatttaagaa actggtgtgg taattttagt
       61 gtcagtaatc gagttatcga tgaagttatg tcactaggca caccagtttt cttattatgt
      121 ggtaggagtt attatgaaat taaatgaagt aagagtaaaa gattttaatt ttggagatgt
      181 tgatggaagt gatgaatcgt tagaacctaa atttcaagat ttattttatg atgaagattc
      241 tttttatgaa aaagagctaa gcaaaaaaaa gaaatttatc atttatggca gaaaaggtac
      301 tggtaagact ttattagctt catacctaaa tgaaaaaatg agacgtaatg ctaatcagtc
      361 tagtaaaata gtttcctatg atgaatatat ctatgagaaa ctgaaagagt tcaagtatga
      421 ttctgttaag aaggaagaac aatcattgtt ttggagatac gtatttttgc aacagtttgt
      481 tgacttaata aaaaaagatt atgagtatgc ccgtttttgg caattttata ggaagcggaa
      541 aataaaaaaa ctaattgaat cagtgacacg acaattttta gaggttcaac aatttataaa
      601 tgaagattct acggaaatta aaaatagcgg gaaatttaac tcaaaaatat ctgatttagg
      661 tgcggaatta cagg
//
LOCUS       DAVGJJ010000175          657 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000175 DAVGJJ010000000
VERSION     DAVGJJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.177"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..597)
                     /locus_tag="VVE35_002041"
     CDS             complement(<1..597)
                     /locus_tag="VVE35_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU3062141.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCSDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDE"
BASE COUNT          223 a          130 c           80 g          224 t
ORIGIN      
        1 ttcatcaagt tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat
       61 actactatca cttacatatg ggaaatcctc ataatatatt actttataac gcataaattc
      121 aagcgccctt ccaatactat tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg
      181 acaatatacg acacatttat cgtctaaatg cattaacagc tcttttatga tatcattctt
      241 taatgtgtcc tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct
      301 atctttccta tagagacatt catagtacga taagtgtcta aaatcacatt gtagacgttc
      361 acaagctaac ctgtcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa
      421 cttatgaaat tgcttagcag agggctgtag ctgttggctc aaagggtaac cagaaaatat
      481 agtaataaca agtacaattt ctccttctga agttaatttt gaaatataat cactacagga
      541 aaaaattgcg tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac
      601 cattccctct acaatttatc taaaaactca ctaagtgtct gattaaattc cacatca
//
LOCUS       DAVGJJ010000176          583 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000176 DAVGJJ010000000
VERSION     DAVGJJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.178"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(5..484)
                     /locus_tag="VVE35_002042"
     CDS             complement(5..484)
                     /locus_tag="VVE35_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU3062142.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          157 a          138 c           76 g          212 t
ORIGIN      
        1 cttactagtg ggtacggata gtaaactcct taaacacaat gcgtaagtct cttaatactc
       61 tttgacgccc tcgaaatcgt tcattgctta agacgcgttc tagttcttct tgtttctctt
      121 tactttgttt gtttctataa tctcttagtg tttcatgaaa gagataataa tcatctagcc
      181 agagaccccc ctcacttata cgatgactga tctcacccac ttcttcatag ggttctttat
      241 cgtatttacg cttctgactt tcctgcttac ggatatagtc taaaattcga tttcgaaact
      301 tggttttgaa atatttcctt aaacgtggaa tatcgtctac cagtccttct tctctactaa
      361 tcaattcatg taagcagagc atgccttctt ggtcccaatc cgacaattcc cataaatgaa
      421 ggtaatattc atttctacac ttatacacag tcccttggac ttcttcatac aattctttaa
      481 tcataatttt cccctttcta gatacagtct aacagattag aaaacacatt tgttccattt
      541 tctccattct gttcccttta gctcctaaac tgcacaaaaa aga
//
LOCUS       DAVGJJ010000177          577 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000177 DAVGJJ010000000
VERSION     DAVGJJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.179"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          221 a           75 c          113 g          168 t
ORIGIN      
        1 gaaaggcact attaaagact ttaagtctaa aaaggtatca aagtatgcca gagataaaaa
       61 aatataaata tgacacttta aaggcgatta gaaaattaaa gaaatgctag aagactttta
      121 gatgacagtt accaagaggg tctaattttc actgctactt atgcagtggg aattggacct
      181 ttttatataa gcaaattcta tttcaaaatt ggttaccaag aaagacttct tttcactgtt
      241 aagtataaga gggtaattta tagaattttg ttacatcaag ataaatattt tctgatggtc
      301 aaattttaaa aagattaata actctcgata aattttgaac cttaacaatt gaatagacca
      361 agacaagacg ttaattattt ttggagcgta ataacctatc cgtcaagatc tttcggctga
      421 ataattcggc aaaaaaagtt ctgctaagca aaaagaaagt gagcgataag taagagtttg
      481 aatataggga gggataccct atccgctatg aagaaaatga ggataatgat acttctaagg
      541 ttgaagtcgg ctcatccgga atagaggcgg aggtgag
//
LOCUS       DAVGJJ010000178          567 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000178 DAVGJJ010000000
VERSION     DAVGJJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.180"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(29..307)
                     /locus_tag="VVE35_002043"
     CDS             complement(29..307)
                     /locus_tag="VVE35_002043"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU3062143.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSA"
     gene            complement(317..565)
                     /locus_tag="VVE35_002044"
     CDS             complement(317..565)
                     /locus_tag="VVE35_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU3062144.1"
                     /translation="MEKLAKKYSDNLNIKVQERVREMANDNKSHYLIYRVLGISFEEG
                     ENIDLYQNKGRFLYKYAGSFLEEAAVLSFNEKFGTENT"
BASE COUNT          164 a           99 c           73 g          231 t
ORIGIN      
        1 caataccggt cactgctctt aaatgttctc aggcagaatc tccataataa tatttccctc
       61 caataccgtt atacaatgtt tctaaagttt gctgaatttt tatagcttga gttctatttg
      121 gatagtagaa cattaaccga attggtatat atcctttgtt atgaataact tttattctag
      181 tgtgttcttt agttatatgg tctccatctg tagttgcatc ccaccatttt atttcgtatg
      241 cgtgtttttc tccgactaaa caatcaattt caaaagtctt aggttttgta ctttcagaat
      301 taggaatgtt aactttttaa gtattttctg taccaaattt ttcgttaaag gatagtactg
      361 cagcttcttc taaaaatgaa ccagcatatt tgtataaaaa acgaccttta ttttgataca
      421 aatcgatatt ttctccttct tcaaatgaaa tacctaaaac tctgtatatc aaataatggc
      481 tcttattatc atttgccatt tcacgaactc tctcttgaac tttgatgttt aagttatcag
      541 aatatttctt agctaatttt tcgattt
//
LOCUS       DAVGJJ010000179          525 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000179 DAVGJJ010000000
VERSION     DAVGJJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.181"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            24..>525
                     /locus_tag="VVE35_002045"
     CDS             24..>525
                     /locus_tag="VVE35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU3062145.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRR"
BASE COUNT          186 a           65 c          110 g          164 t
ORIGIN      
        1 gaaaacatac gttttaggag ggaatgaatg tgttaaacat taaagagaaa gatatccttc
       61 aatttttaat aaaaaataag gaacgttttg ttacaagcaa agaactggca gaatatttat
      121 cctgttcaga tagaactgtc cgtaatgtac tgaaattaat tgagaagaca atgattattc
      181 aaggagtccg gttgatttca aaacaaggtc aagggtatca aatatttttt gagaatcaag
      241 gcgcttacca ggagtttaga caaacttatg aactggaaga agactatacg aaaactgcgg
      301 tttcaaaagg tgatgatcgt ttagtattta tcttaaataa attattattt gagcaagttc
      361 cagttctttt tgatgattta gctgatgaac tttacgttag tcgttcaaca ttatctcatg
      421 atttcagaaa gatacgagtg atgttatcag agtataattt gtccattgag agtagggcga
      481 ataaaggagt ttatgtctca ggcgaggaac gtgataaacg acgtt
//
LOCUS       DAVGJJ010000180          520 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000180 DAVGJJ010000000
VERSION     DAVGJJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.182"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..418
                     /locus_tag="VVE35_002046"
     CDS             <1..418
                     /locus_tag="VVE35_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU3062146.1"
                     /translation="KNKVDQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT          200 a           81 c          106 g          133 t
ORIGIN      
        1 taaaaataag gtagaccaag atagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag gctgaaattc ctcaagtaga gcattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta
//
LOCUS       DAVGJJ010000181          519 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000181 DAVGJJ010000000
VERSION     DAVGJJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.183"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          221 a           75 c           64 g          159 t
ORIGIN      
        1 ggaatcaata acaaattcaa caaaatactt tttatatcat tactataact tgtcaccgga
       61 atcaaacata acaacaatga aaattgtaag aaagagatta aatgcgtcaa catcattagt
      121 tgtgcattct taaaaaagaa attcatttta gaatgattaa gagcagataa ttgaagtaaa
      181 gctagatcta ccccaattga ggatttatga ttccctatta ttgcctccat taatgtaata
      241 tatattgagg tataaagaaa taatattggt atttttgtgt catcaattga taacatcgtc
      301 atcatatacc aaaatagata ggaaagaaga atactaatca aaataaaaga aaaagttaac
      361 cctttattta accaacaaaa acgaagcaat ataaatagac tgctacgaaa tcttttttga
      421 ctcattcgac tgcttttaaa atagaaatta ctcgcatgat tatctaatat ccccatgact
      481 aaaataaaat aattgagtag tatgacataa attacaact
//
LOCUS       DAVGJJ010000182          493 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000182 DAVGJJ010000000
VERSION     DAVGJJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.184"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          155 a           70 c           90 g          178 t
ORIGIN      
        1 aaaggcagtc cgaagaccgc caatatttat ctctctgcac ctttgctatg gtctttctta
       61 tttgacttag atttgtcatc tgtcttaaag ctttttattt gccctaatat ggacttttta
      121 tccttgtctt gactctttac ttgttctttt gcttttaaga gcttagaaga caaaaagaga
      181 tattatatta aaacaatatt tctccggtac tgggactggg acgggactaa aaatctgaaa
      241 acgtcctata attaggtgga tttagtgata ttataaaaaa tagaatctag tattttgaat
      301 ggttttggat atattgtacg tttggaaaag aagaatggga gtaattttaa actaacaaaa
      361 aatgctttga aatcagtgtt tcagagcatt tttgggctct ataatatttg tagtgggtaa
      421 atcccctata gatattatgg agcctatttt tgtgtagaaa aaaagtccca tatgacctat
      481 aatgaaaagc gac
//
LOCUS       DAVGJJ010000183          492 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000183 DAVGJJ010000000
VERSION     DAVGJJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.185"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            122..340
                     /locus_tag="VVE35_002047"
     CDS             122..340
                     /locus_tag="VVE35_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU3062147.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
BASE COUNT          214 a           56 c           89 g          133 t
ORIGIN      
        1 ataaggacgg aataacacaa caaattctct aaaacaaatc actaaatcaa tgtaagattg
       61 aatgaaatca atatttatgc tataattaaa taaatttaat gaagaaaaaa agagggatat
      121 tatggcactt aactataaac cattatggat acagttagca aaaaaaggac taaagaaaac
      181 agatgtaata gctatggcag gacttacaac aaatgttatg gcacaaatgg gaaaggataa
      241 accaattaca tttaagaatt tagaaagaat atgtaaggct ttatcttgca ctcctaatga
      301 tattattagt tttgaagata attttagtga cgaggaatag aaaatgactt taaggacaga
      361 agatcaagtt agggattatg caagagaagt ataggcttta atgaagttga agaaaacatc
      421 aatcaaggta ctggtcaaat aactactttt aatcaattag gcttcaaggg atattcagat
      481 aagccagatg gt
//
LOCUS       DAVGJJ010000184          490 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000184 DAVGJJ010000000
VERSION     DAVGJJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.186"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            113..>490
                     /locus_tag="VVE35_002048"
     CDS             113..>490
                     /locus_tag="VVE35_002048"
                     /inference="COORDINATES: protein motif:HMM:NF016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU3062148.1"
                     /translation="MTETNKQLHETLIEILDFVKEICEKHELTYFLVFGTALGAKRHC
                     GFIPWDDDVDIALPREHYNIFIDALSETDQSIFSLQNEDNEPNYFLPFAKLRKNNTIF
                     IEKILDVEYENNGIYIDIFPLDFV"
BASE COUNT          175 a           65 c           84 g          166 t
ORIGIN      
        1 atttatactg attcgttatc ttactatctc actcatccta ggaggatgtt ttttaaagag
       61 gaaaaagata atgagttcga tagctatcat taaacaacag tgaggtacaa gaatgacaga
      121 aacaaataaa cagttacatg aaacattaat cgaaatttta gactttgtaa aagaaatttg
      181 tgaaaaacat gaattaactt attttttggt ctttgggaca gcacttggag caaaacgcca
      241 ttgtggattt attccatggg atgatgatgt tgatattgct ttgccacgag agcactacaa
      301 tatatttatc gatgcattat ctgagacgga tcaatctata tttagtcttc aaaatgaaga
      361 taacgagccg aattattttt taccatttgc taaattaaga aaaaacaata cgatttttat
      421 tgagaagatt cttgatgttg aatatgagaa taacggtatt tacattgata tttttccact
      481 agatttcgta
//
LOCUS       DAVGJJ010000185          485 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000185 DAVGJJ010000000
VERSION     DAVGJJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.187"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>485
                     /locus_tag="VVE35_002049"
     CDS             <1..>485
                     /locus_tag="VVE35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU3062149.1"
                     /translation="TWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLI
                     LDNIFGADMFQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTN
                     IDQILVERKRDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPT
                     Q"
BASE COUNT          195 a           61 c           78 g          151 t
ORIGIN      
        1 acgtggactt cgattgagga ttacaaagaa tttttgtctg taagattaga agaatgcaaa
       61 agagtgctaa aaaatagtgg cagtattttc gttcattgtg ataaaattgc aaatcatcat
      121 attagattaa ttttagataa tatctttgga gcagatatgt ttcaaagcga aattatatgg
      181 aactataaac ggtggtctaa ttcaaaaaag ggattattga acaatcatca aaacatttac
      241 ttttattcaa agtcaaaaga ttttaaattt aatacaattt ttacagagta ttcttctact
      301 acaaatatcg accaaatact agtggaacga aaacgagatg gaaactctaa aactatatat
      361 aaggttgata ataatggtaa ctatattcta gcaaaagaga aaaatggagt tcccctttca
      421 gatgtttgga atataccatt tcttaatcca aaagctaaag aaagagtagg ttatcctaca
      481 caaaa
//
LOCUS       DAVGJJ010000186          483 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000186 DAVGJJ010000000
VERSION     DAVGJJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.188"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            19..>483
                     /locus_tag="VVE35_002050"
     CDS             19..>483
                     /locus_tag="VVE35_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU3062150.1"
                     /translation="MPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVRE
                     FLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYS"
BASE COUNT          178 a           51 c           98 g          156 t
ORIGIN      
        1 gacgatgatg atgaatatat gccagatcga attttaaaat tgatggcttg ttttaaaaag
       61 agtagaatga agaatttagc tttagtttat agttatggaa taataattta tccaaatggt
      121 acacgagaag aggagaagac cgattttgtt ggaaatccct tgtttgttca aatggttcac
      181 aatatagcag gtacgtcatt ttggttgtgt aaaaaagagg tgctagaatt aattaatggt
      241 tttgagaaaa tagattcaca tcaggacggt gttgttttat taaaactact tgctcaagga
      301 taccaaattg atatagtgcg agaattcttg gtgaattact acgctcacag taaagaaaac
      361 ggtatcactg gagtgacaca aaaaacaatt aatgcagatg aagaatatta taattactgt
      421 aggaaatatt ttaatttatt gagtttcaac gagagaatat tggttacaaa gaaatattat
      481 tct
//
LOCUS       DAVGJJ010000187          460 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000187 DAVGJJ010000000
VERSION     DAVGJJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.189"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          165 a           52 c           76 g          167 t
ORIGIN      
        1 tattaatatc cctgatggat atggagacat taaggatata gtgttttcga ctcatattat
       61 agttaggtat aataatggac attgtggagg tatagatccg catataattg gactttgtaa
      121 aaagcagatc agaaggatgt ctctatatcc tattttgata atcgtatcta gagattcaaa
      181 ggttattgat gattataaaa atttagatat tgcctatgtt gattgtactc aatgttcaaa
      241 taattttgaa actgccttac acgttaaaaa tattttaaaa ttattaaaaa tccaacttat
      301 acactgtcat ggctactcaa ctaattattt tttatatatg ttaaaaaaac ttgacaagaa
      361 tggatttgga aaggttaaga cagtgataac atgccatgga tgggttgaat ataacttaaa
      421 aaagaaattc ttgacttatt ttgatttttg gacatattct
//
LOCUS       DAVGJJ010000188          459 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000188 DAVGJJ010000000
VERSION     DAVGJJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.190"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>459
                     /locus_tag="VVE35_002051"
     CDS             <1..>459
                     /locus_tag="VVE35_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU3062151.1"
                     /translation="PDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLI
                     PNKTAESVNQALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSN
                     ENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          148 a           86 c           93 g          132 t
ORIGIN      
        1 gtcctgacgt tattaatctt cgcttggaaa atggtcatta tgaaattgat accgtcctac
       61 tgactaagat aaaaaattat tgcctgttag tcttaaccga ccggcggagc agacaccaaa
      121 ttataaggtt aattccaaat aaaactgctg aatctgtcaa tcaggcgctt acgttactat
      181 taggggagca tcgtattctg tccattactg cagataatgg ttcggagttc aaacgattgt
      241 ctgaggtatt tcctgaggaa catatctact acgcacatgc ttactcttca tgggagagag
      301 gttcaaatga aaatcataat cgattaattc ggagatggtt acctaaagga accaagaaaa
      361 cgactccgaa agaagtagct tttatcgaaa attggattaa caactaccct aaaaaatgct
      421 tggactacaa gtcgccaagt gaatttcttt tgggtggct
//
LOCUS       DAVGJJ010000189          455 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000189 DAVGJJ010000000
VERSION     DAVGJJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.191"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..339)
                     /locus_tag="VVE35_002052"
     CDS             complement(<1..339)
                     /locus_tag="VVE35_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU3062152.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEH"
BASE COUNT          143 a           82 c           68 g          162 t
ORIGIN      
        1 atgttcttta gctgatttta agatggcttt agcaaattca aactgagcaa atatttcacc
       61 tcctgataaa gttaaacctc caccggattc ttcgtaaaat tctttgtctt ttaatacctc
      121 tgtaataatt tcttctacag tcttttcttc accgactaag gtgaatttct ctcgttgagc
      181 atctttcatt ttttcaggtt tcattctttg agattcagga ttagaacacc atggacagcg
      241 cagaggacat ccttttaaaa aaacagttgt acgaataccc ggaccgtcat gaattgaaaa
      301 gtgttgaata ttaaaaataa ttcctttaga tatttccata agagttcctc ctcttcataa
      361 gtctatccta tcacaaaaac gaaagaaaaa caattacgaa cgaaaggtta atttgttgtt
      421 ttttgctttt tattttggta gaataaaacg gagat
//
LOCUS       DAVGJJ010000190          453 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000190 DAVGJJ010000000
VERSION     DAVGJJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.192"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            183..413
                     /gene="blpU"
                     /locus_tag="VVE35_002053"
     CDS             183..413
                     /gene="blpU"
                     /locus_tag="VVE35_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU3062153.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          161 a           50 c          111 g          131 t
ORIGIN      
        1 taaaaaagtg attagaaaac atctttttta aaaatagaga tgattttgaa acaaaaaagc
       61 taattcaaga cgtttcgatg ccaattcaag atttggatga aaaaaattaa tagatactgt
      121 tatactaaac ttgtcaagtt tgtaacaaga caaatattaa aaataaaaaa gaggtattcg
      181 ttatgaatac aaaaacgatg tcacaatttg aaattatgga tactgagatg cttgcttgcg
      241 ttgaaggtgg cggatgcaat tggggagatt ttgccaaagc aggtgttgga ggaggagcag
      301 cacgaggtct tcagctagga attaaaacaa gaacatggca aggtgcagca actggtgctg
      361 tgggaggagc tatacttgga ggtgtggcct atgcagcgac atgttggtgg taattatgga
      421 ttttaaaagt tttattattg gtttagtagt tgg
//
LOCUS       DAVGJJ010000191          451 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000191 DAVGJJ010000000
VERSION     DAVGJJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.193"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            140..436
                     /locus_tag="VVE35_002054"
     CDS             140..436
                     /locus_tag="VVE35_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062154.1"
                     /translation="MVKINKICSIQGSSVENEDTVGSQNQYFWIIDGATDLYNFKEEI
                     GYSVSEVVHILSKSLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT          187 a           45 c           80 g          139 t
ORIGIN      
        1 gaaaatcata ttaataaatc taaagaaata gtagatgtaa aacaaaaatt accaaagact
       61 ggttcgaaca agattatgga actattttta acagtgacag gaattggttt acttttgaca
      121 ctaaaagggt tgaagtatta tggtaaagat aaataaaatt tgttcgatac aaggaagttc
      181 cgtagaaaat gaggatactg taggttcaca aaatcagtat ttttggatta ttgatggagc
      241 tacagattta tataatttta aagaggagat aggttattca gtctcagaag ttgtacatat
      301 tttatcaaaa agtttgtcgg ttaattgtaa agaatcaaaa actcttaaac aaatatttga
      361 aactgcttta ctcgaggtta aagatgaaat cggtttaaac tcatatgaat taacagagta
      421 tagcaaaatg aaataataat aggacaaatc a
//
LOCUS       DAVGJJ010000192          448 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000192 DAVGJJ010000000
VERSION     DAVGJJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.194"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            189..425
                     /locus_tag="VVE35_002055"
     CDS             189..425
                     /locus_tag="VVE35_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU3062155.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT          132 a           36 c           87 g          193 t
ORIGIN      
        1 aagttttaag gcggtctttt tagaacttta attgtttgaa atttaggtag caaatttgtt
       61 tctattttgt caacttttcc tatttttatc ttgttgaggc tggtatttta acaattcagg
      121 aattgatagt gaatgtgtaa aattttttgt tagaataagt ttataaaaaa gaaaaggagt
      181 atttgattat gttacaaaaa atttatgagc agatggctaa tttctatgat agtattgaag
      241 aagagtatgg tcctacattt ggtgataatt ttgactggga acatgttcat tttaaatttt
      301 taatttatta tttagtgaga tatggcattg gttgtcatag ggattttatc gtttaccatt
      361 atcgtgttgc ttatcgtttg tatcttgaaa aattggtaat gaatcggggt tttatttctt
      421 gttgaggtaa ttttagtaaa tttccgaa
//
LOCUS       DAVGJJ010000193          441 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000193 DAVGJJ010000000
VERSION     DAVGJJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.195"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..428)
                     /locus_tag="VVE35_002056"
     CDS             complement(<1..428)
                     /locus_tag="VVE35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062156.1"
                     /translation="MKLNKLNFLKENIRDLYSSGVIYLGLIISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNT"
BASE COUNT          171 a           77 c           60 g          133 t
ORIGIN      
        1 attgtattga ctacaatagc tactataaaa tctaatccta aatcacctgg aatacccaat
       61 ttacttacta tgactagtaa cacaatgcca ttcaaagcat aaataatgga aactgagatg
      121 gcatatagaa aattagacaa gacgtaaatc atccgattct tctgttgatt ataaaacaaa
      181 cttaccgtat tgtgggaaaa atctctacta atgactcgat tagcatgtat aacagccatt
      241 aacataccga gcatagcata aaagtttgaa atatgcttaa taccaagcga tgtcccttga
      301 gtctttagaa taaagaatgt aagcaatatc ggcggtataa acgagataat caaaccgaga
      361 tatattacgc ctgatgaata taaatccctt atattttcct taagaaaatt caatttattt
      421 aatttcatga ttataacctt c
//
LOCUS       DAVGJJ010000194          441 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000194 DAVGJJ010000000
VERSION     DAVGJJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.196"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            330..>441
                     /locus_tag="VVE35_002057"
     CDS             330..>441
                     /locus_tag="VVE35_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062157.1"
                     /translation="MTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGE"
BASE COUNT          130 a           87 c           89 g          135 t
ORIGIN      
        1 tgaattgtca aattaagtgc ataagtttcc cgtagcatac ttcataaggc gttttccact
       61 taagaaattt tcgtggtcga ttatttagaa cttgtattca caaaaaatgt taaactagag
      121 ttatgacacg caaagcatat gatactgatt taaacgatca agaatgggct aagattgaac
      181 cctatttctc caaacaccgc acctataaat ggccgaaacg agtacttgtt aatgagactt
      241 tgtacgtcac taaaacaggt tgtcaatggc gcatgatccc tcacgacttt cctctttact
      301 taacggtatg gagtttcttt cgtcgttcta tgacgacagg ttggttccag gtcaatggca
      361 gatggtacta cgcttatagc tcaggtgcct tggcagtgaa tacgaccgta gatggctatt
      421 ctgtcaacta taatggcgaa t
//
LOCUS       DAVGJJ010000195          409 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000195 DAVGJJ010000000
VERSION     DAVGJJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.197"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            complement(<1..368)
                     /locus_tag="VVE35_002058"
     CDS             complement(<1..368)
                     /locus_tag="VVE35_002058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062158.1"
                     /translation="MGGIGLVYTIWSDRPFTALNLFRIYMFPAVIYFIIINCNFRKER
                     LLILKQAHVYTACILALWGIFQAWVLKDQFLIKIGYPSQGNFLKSTAFYIGGFFGQQR
                     VTSTFSAPNLAGVYFGISLI"
BASE COUNT          157 a           81 c           50 g          121 t
ORIGIN      
        1 atgatcaaag atattccaaa ataaactcca gctaaattag gagcagaaaa agtgctagta
       61 actcgttgtt gaccgaaaaa accaccaata taaaaagctg tactctttag aaaatttcct
      121 tgggaaggat atcctatttt aatcaaaaac tgatctttta acacccacgc ctgaaaaatt
      181 ccccataaag ctaatatgca agcagtatat acatgcgctt gcttcaatat cagtaatctc
      241 tccttgcgaa aattacaatt tattattata aaatatatta cagcaggaaa catataaatt
      301 ctaaaaagat ttaaagctgt aaatggccta tctgaccaga tagtataaac taaaccaatt
      361 cctcccataa caattattgg gccccctagc tttccaattt taatttttc
//
LOCUS       DAVGJJ010000196          394 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000196 DAVGJJ010000000
VERSION     DAVGJJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.198"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            77..391
                     /locus_tag="VVE35_002059"
     CDS             77..391
                     /locus_tag="VVE35_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU3062159.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
BASE COUNT          146 a           56 c           77 g          115 t
ORIGIN      
        1 ttttataata aaaatagata acaaataagc caacttagca cagctttgat tggatttaag
       61 aaaaggagaa aatattatgg caaaagcttt aattatttgt gcagcaggaa tgtcttcatc
      121 acttatggca cagaaaacaa cagagtttct aaaaggaaaa gggaaagata ttgtagttga
      181 tgctattagt gctactgaag gagggaaagc tatcgcagca gcagcttttg atttgtatct
      241 tgtcagtcct caaacaaaaa tgtactttaa gcaatttgaa gaggctggtt ctaaagcagg
      301 aaaaccagtt gtacaaatac ctcctcaagc ctatattcct attccaatgg gaattgaaaa
      361 aatggctaat ttgattcttg agaatatttg atga
//
LOCUS       DAVGJJ010000197          362 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000197 DAVGJJ010000000
VERSION     DAVGJJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.199"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          152 a           58 c           50 g          102 t
ORIGIN      
        1 ccctcaataa tcggtataaa agatactgac tcataccttg ataccaaata aaattcggta
       61 tttcaataag agcatgaggt aatattcctt ttattgtatc tactattcca atcatctgac
      121 cagatatacc caattgtatt cctaaagata gcaaatcaaa aagttgcaat aagggcgaaa
      181 tcggaaaaca gaatagatat agcaaaaaat ttgtagaatt tgcctgtaaa actgtcaatg
      241 taaattgtaa ttccagtttc ggaggtatag tagcaaaagt ctcatttttt aatataaagc
      301 ttaaaagtaa acctagtaaa aaacagacta tacaaaataa cacatagtaa atcagaaaac
      361 ca
//
LOCUS       DAVGJJ010000198          358 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000198 DAVGJJ010000000
VERSION     DAVGJJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.202"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            239..>358
                     /locus_tag="VVE35_002060"
     CDS             239..>358
                     /locus_tag="VVE35_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU3062160.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKA"
BASE COUNT          121 a           58 c           82 g           97 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcg
//
LOCUS       DAVGJJ010000199          358 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000199 DAVGJJ010000000
VERSION     DAVGJJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.203"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          136 a           62 c           59 g          101 t
ORIGIN      
        1 ttcttttata taataatgtc cttgaaagta gtttcagctg aacatttata gtccataaaa
       61 agaaaacaga aactcatctg tttttatact caatgaaaat caaagagcaa actaagaaac
      121 tagccgcatg atgctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
      181 accataccta cggcaaggca aagctgacgt tgtttgaaga gatttttgaa aagtattaac
      241 acctacagac aagcctgacg atagcaagaa ctaccctact cgataggtat cggcttttgc
      301 tttctgaaaa aaattatttt aagcaatttg acaaatctag caacaaaaaa ttctataa
//
LOCUS       DAVGJJ010000200          353 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000200 DAVGJJ010000000
VERSION     DAVGJJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.204"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          120 a           70 c           45 g          118 t
ORIGIN      
        1 cctcttaagg tgcgaaatta ttcaagttct taaaaaatgc ttctctattt ccgtaagata
       61 gcttatttct tcgatttaag aactgaagat gatagttcta acctttagaa atccaagcct
      121 tcactagctc aaaaactcct atctttcttc ctcaataaat catctgagag ataggtattc
      181 cttatgtcct tcaaattcat cactcctttg gatattgagg tgctagccta gaagcagaca
      241 cccgattatc taaaccatcc ttaataaaga tgacaagaca gataagctca gttaattact
      301 tatatatctt tttaatatgg tatatttgtc aacaaaaaga aatcaaatca tac
//
LOCUS       DAVGJJ010000201          338 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000201 DAVGJJ010000000
VERSION     DAVGJJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.206"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            <1..>338
                     /locus_tag="VVE35_002061"
     CDS             <1..>338
                     /locus_tag="VVE35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU3062161.1"
                     /translation="MLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQLA
                     LDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLI
                     SKETVQKAVA"
BASE COUNT          122 a           76 c           66 g           74 t
ORIGIN      
        1 atgttgactc gtctcttgct caaggcatct gacaaggcac catggtcaga taacggtgac
       61 gctaaaaacc cagccctatc tccactaggt gaaaacgtga agaccaaagg tcaatacttc
      121 tatcaattag ccttggacgg aaatgtagct ggcaaagaaa aacaagcgct cattgaccag
      181 ttccgagcaa atggtactca aacttacagc gctacagtca atgtctatgg taacaaagac
      241 ggtaaaccag acttggacaa catcgtagca actaaaaaag tcactattaa cataaacggt
      301 ttaatttcta aagaaacagt tcaaaaagcc gttgcaga
//
LOCUS       DAVGJJ010000202          321 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000202 DAVGJJ010000000
VERSION     DAVGJJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.denovo.207"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
BASE COUNT          107 a           67 c           41 g          106 t
ORIGIN      
        1 cttcgactgc actttttttg gcaaaaaata gacagaacaa acatcctacc tactaaaaat
       61 atgatatgaa aggaaaatga ttttttactg acaccctagg caagcttggg acaaataaat
      121 cattgatagc aacatttacg acatcctatt taaagttcct tcaatttcta atgtccccgc
      181 tatttctaag ccacctctgg tatgatctat tcctagtcag aatagataac accgttctaa
      241 attacgattc atagttactc cttttttgac tcgttactat aagactccta tttacatgcg
      301 caaaataatt ttgattcaga t
//
LOCUS       DAVGJJ010000203         3001 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain SP3472
            SAMD00182594-rid19693183.guided.120, whole genome shotgun sequence.
ACCESSION   DAVGJJ010000203 DAVGJJ010000000
VERSION     DAVGJJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00182594
            Sequence Read Archive: DRR189357
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001925885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001925885.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2023 17:55:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,064
            CDSs (total)                      :: 2,017
            Genes (coding)                    :: 1,878
            CDSs (with protein)               :: 1,878
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 66 of 139
            Pseudo Genes (incomplete)         :: 61 of 139
            Pseudo Genes (internal stop)      :: 35 of 139
            Pseudo Genes (multiple problems)  :: 21 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00182594-rid19693183.guided.120"
                     /strain="SP3472"
                     /db_xref="taxon:1313"
                     /country="Japan"
                     /collection_date="2018"
     gene            206..1945
                     /locus_tag="VVE35_002062"
     CDS             206..1945
                     /locus_tag="VVE35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU3062162.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWAANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            2220..2522
                     /locus_tag="VVE35_002063"
     CDS             2220..2522
                     /locus_tag="VVE35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU3062163.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSRNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKLVSINE"
     gene            2578..>3001
                     /locus_tag="VVE35_002064"
     CDS             2578..>3001
                     /locus_tag="VVE35_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU3062164.1"
                     /translation="MDGITKDFIKTAKLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFT
                     EVEGDTIVGLALCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAK
                     NKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHFKKK"
BASE COUNT         1007 a          451 c          627 g          916 t
ORIGIN      
        1 gattgtacca tacttgtaaa ctttatatcc ttctaatcaa tagtagtaat tcaaagcctt
       61 ttttaaggct ttttgtatac tttaaaagat agttccttta acaacggaca ttccttgcaa
      121 atagtttaca aaaatagtat actggattca ttgagtttga aaacgtttgc gtaaaatttg
      181 aatgaatact ttaggagaca aattgatgga attgagtgct atttaccata ggcctgagtc
      241 ggagtatgcc tatctttata aggataagaa actccatatt cgaattcgaa ctaagaaagg
      301 ggacattgaa agcatcaact tgcactatgg ggaccctttt atctttatgg aggagtttta
      361 tcaggataca aaagaaatgg tcaagataac ttctggtacc ttatttgacc attggcaggt
      421 tgaagtgtca gttgactttg cacgtatcca gtatctcttt gagctcagag atacagaagg
      481 tcaaaatatt ttgtatggcg ataaagggtg tgtggaaaat tctctagaaa atcttcatgc
      541 aattgggaat ggatttaagt tgccttatct tcatgagatt gatgcctgca aggttcctga
      601 ctgggtttca aatacggtat ggtatcagat atttcctgaa agatttgcca atggcaatgc
      661 tctattaaac ccagaaggga ctttagactg ggattcatct gtcacaccta agagcgatga
      721 tttctttggt ggtgatttac aggggattat tgatcatatg gattacttgc aagacttggg
      781 tattactgga ctatatcttt gtcccatctt tgaatctaca agcaatcaca agtacaatac
      841 gacagattac tttgaaattg accgtcattt tggagacaag gagacctttc gggaactggt
      901 ggatcaagcg catcatcgtg gcatgaaagt catgctggat gcggtattta atcatattgg
      961 ttcgcaatct cttcaatgga aaaatgtcgt caaaaatggt gaacagtctg cttataagga
     1021 ttggttccat attcaacaat tcccagtgac aactgaaaag ctagttaata agagagactt
     1081 accctatcat gtttttggtt tcgaggacta tatgcctaag ctaaatacag ccaatccaga
     1141 ggtcaagaat tatcttttaa aggttgcgac ttattggatt gaagagttta atatcgatgc
     1201 ttggcgtttg gatgtggcta atgagattga ccatcagttc tggaaggatt ttcgtaaggc
     1261 agttttagct aaaaatcctg atctttatat cctaggagaa gtctggcata catctcagcc
     1321 ttggctaaat ggagatgagt tccatgccgt catgaattat cctttatctg atagtatcaa
     1381 ggactatttc ttacgaggaa ttaagaagac agaccagttc atcgatgaaa tcaatggaga
     1441 gtctatgtat tacaagcagc agatttcaga ggtcatgttt aatctcttgg attcacatga
     1501 tacagagcga atcctgtggg cggccaatga agatgttcaa ctggttaaat cagccttagc
     1561 ctttctcttt ttacaaaaag gaacaccgtg catttattac ggaaccgagc tagccttgac
     1621 tggaggacca gatccagatt gtcgtcgttg tatgccttgg gaacgtgtat caagtgacaa
     1681 tgatatgctg aactttatga agaggctgat taaaattcgg aaatacgcgt cagtaatcat
     1741 ttcgcatggc aagtatagcc ttcaagaaat caaatctgat ctagtagctc tggaatggaa
     1801 atacgaagga cggatcctca aagcaatatt caaccaatca acagaagatt atcttttaga
     1861 gaaagaagca gtagcactag caagcaattg ccaagaattg gagaatcagc ttgtcatctc
     1921 tccagatgga tttgtgattt tctaaaaact agttgatgaa gattatggta catttcatat
     1981 cttatatagt ataataaggt tagttactaa acttgtaaag gagaacttaa atgaattgta
     2041 gaggacatga aacaagacaa agaattgtta gagattttga agtttagcct aaagcacata
     2101 ttaagctgtt agcaaatcaa caaaaacata gtgatgcagg agcaactatt gaagatgaat
     2161 attatgtatt tatcgctgag agtaaaattg atggcaagaa ggaagttatt cagtgttgca
     2221 tgggtgcggc aagggatttt ttagaactaa ttaatcacaa agggctacct ctttttaatc
     2281 cgcttgtagg tgattctcat gtaaataata gacaagaata tgacaataca gggagtagaa
     2341 atttacaacc tgaaaagtgg aatgaaactg caaagcagct ttataatgct ataatgtggt
     2401 tgattatttt atggaatgct aagccggata cacctttatt taattttaaa gacgaagtaa
     2461 ttaagtataa aacatatgag ccttttgaaa gcagtataaa attggtatca atcaatgagt
     2521 aacaaagtat cagcatgatt cgaaacattt gcttgtaatt tgcggaggaa catcaatatg
     2581 gatggaataa caaaagattt tataaaaacg gctaaactaa tgaaacaatt atggccccaa
     2641 ttgaccgata aagaagctat tgatgaagta aaaaaatata cgaatggcaa aaatactgca
     2701 atctttactg aagttgaagg tgacacaatt gtaggtctag cactatgttc actcagattt
     2761 gattatgttg aaggttgtaa atatagtcct gttggattct tagaagggat tattgttgac
     2821 gaggaatatc gtttaaagga tattgctaaa aatctctgta caaaatgtga ggaatgggcg
     2881 aaaaataaag gatgtaagga atttgcaagt gactgtactt taacgaatac ggattctata
     2941 agatttcatc tcaatattgg atttcaggag gcaaatagaa ttattcattt taagaaaaaa
     3001 t
//
